LOCUS       DACGLH010000001       105184 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.001, whole genome shotgun sequence.
ACCESSION   DACGLH010000001 DACGLH010000000
VERSION     DACGLH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 105184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 105184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..105184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.001"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            331..1290
                     /locus_tag="IXT21_000001"
                     /pseudo
     CDS             331..1290
                     /locus_tag="IXT21_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1821..2231
                     /locus_tag="IXT21_000002"
     CDS             1821..2231
                     /locus_tag="IXT21_000002"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4867450.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            2224..2679
                     /locus_tag="IXT21_000003"
     CDS             2224..2679
                     /locus_tag="IXT21_000003"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4867451.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            2696..3427
                     /locus_tag="IXT21_000004"
     CDS             2696..3427
                     /locus_tag="IXT21_000004"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4867452.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            3444..4256
                     /locus_tag="IXT21_000005"
     CDS             3444..4256
                     /locus_tag="IXT21_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4867453.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(4472..5879)
                     /locus_tag="IXT21_000006"
                     /pseudo
     CDS             complement(4472..5879)
                     /locus_tag="IXT21_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5968..6051)
                     /locus_tag="IXT21_000007"
                     /pseudo
     CDS             complement(5968..6051)
                     /locus_tag="IXT21_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6568..7818
                     /locus_tag="IXT21_000008"
                     /pseudo
     CDS             6568..7818
                     /locus_tag="IXT21_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(8392..9246)
                     /locus_tag="IXT21_000009"
     CDS             complement(8392..9246)
                     /locus_tag="IXT21_000009"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867454.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(9263..10261)
                     /locus_tag="IXT21_000010"
     CDS             complement(9263..10261)
                     /locus_tag="IXT21_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4867455.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            complement(10296..11120)
                     /locus_tag="IXT21_000011"
     CDS             complement(10296..11120)
                     /locus_tag="IXT21_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4867456.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            complement(11110..11892)
                     /locus_tag="IXT21_000012"
     CDS             complement(11110..11892)
                     /locus_tag="IXT21_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4867457.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFVLVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            complement(11916..12386)
                     /locus_tag="IXT21_000013"
     CDS             complement(11916..12386)
                     /locus_tag="IXT21_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4867458.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            complement(12404..12826)
                     /locus_tag="IXT21_000014"
     CDS             complement(12404..12826)
                     /locus_tag="IXT21_000014"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAQ4867459.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            complement(12954..15677)
                     /locus_tag="IXT21_000015"
     CDS             complement(12954..15677)
                     /locus_tag="IXT21_000015"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4867460.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FNDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKYNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            15867..16883
                     /locus_tag="IXT21_000016"
     CDS             15867..16883
                     /locus_tag="IXT21_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4867461.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            16991..17860
                     /locus_tag="IXT21_000017"
     CDS             16991..17860
                     /locus_tag="IXT21_000017"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867462.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSAPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            17875..18579
                     /locus_tag="IXT21_000018"
     CDS             17875..18579
                     /locus_tag="IXT21_000018"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4867463.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLELEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            19799..20412
                     /locus_tag="IXT21_000019"
                     /pseudo
     CDS             19799..20412
                     /locus_tag="IXT21_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(21159..24308)
                     /locus_tag="IXT21_000020"
     CDS             complement(21159..24308)
                     /locus_tag="IXT21_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ4867464.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGIKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(24321..25538)
                     /locus_tag="IXT21_000021"
     CDS             complement(24321..25538)
                     /locus_tag="IXT21_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ4867465.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(25528..27123)
                     /locus_tag="IXT21_000022"
     CDS             complement(25528..27123)
                     /locus_tag="IXT21_000022"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ4867466.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            27491..28783
                     /locus_tag="IXT21_000023"
     CDS             27491..28783
                     /locus_tag="IXT21_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4867467.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(29215..29922)
                     /locus_tag="IXT21_000024"
     CDS             complement(29215..29922)
                     /locus_tag="IXT21_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867468.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(29987..30823)
                     /locus_tag="IXT21_000025"
     CDS             complement(29987..30823)
                     /locus_tag="IXT21_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4867469.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(30841..32250)
                     /locus_tag="IXT21_000026"
     CDS             complement(30841..32250)
                     /locus_tag="IXT21_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4867470.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(32264..34087)
                     /locus_tag="IXT21_000027"
     CDS             complement(32264..34087)
                     /locus_tag="IXT21_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4867471.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(34930..35256)
                     /locus_tag="IXT21_000028"
     CDS             complement(34930..35256)
                     /locus_tag="IXT21_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867472.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(35302..35664)
                     /locus_tag="IXT21_000029"
     CDS             complement(35302..35664)
                     /locus_tag="IXT21_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ4867473.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(35701..35915)
                     /locus_tag="IXT21_000030"
                     /pseudo
     CDS             complement(35701..35915)
                     /locus_tag="IXT21_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            36042..36377
                     /locus_tag="IXT21_000031"
     CDS             36042..36377
                     /locus_tag="IXT21_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4867474.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(36453..38450)
                     /locus_tag="IXT21_000032"
     CDS             complement(36453..38450)
                     /locus_tag="IXT21_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867475.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(38548..38823)
                     /locus_tag="IXT21_000033"
     CDS             complement(38548..38823)
                     /locus_tag="IXT21_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4867476.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            39055..39789
                     /locus_tag="IXT21_000034"
     CDS             39055..39789
                     /locus_tag="IXT21_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4867477.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCSNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            39828..40970
                     /locus_tag="IXT21_000035"
     CDS             39828..40970
                     /locus_tag="IXT21_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4867478.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(41065..42456)
                     /gene="sufB"
                     /locus_tag="IXT21_000036"
     CDS             complement(41065..42456)
                     /gene="sufB"
                     /locus_tag="IXT21_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ4867479.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(42461..42931)
                     /locus_tag="IXT21_000037"
     CDS             complement(42461..42931)
                     /locus_tag="IXT21_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ4867480.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(42918..44153)
                     /locus_tag="IXT21_000038"
     CDS             complement(42918..44153)
                     /locus_tag="IXT21_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ4867481.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(44150..45436)
                     /gene="sufD"
                     /locus_tag="IXT21_000039"
     CDS             complement(44150..45436)
                     /gene="sufD"
                     /locus_tag="IXT21_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ4867482.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(45449..46222)
                     /gene="sufC"
                     /locus_tag="IXT21_000040"
     CDS             complement(45449..46222)
                     /gene="sufC"
                     /locus_tag="IXT21_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ4867483.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(46419..47267)
                     /locus_tag="IXT21_000041"
     CDS             complement(46419..47267)
                     /locus_tag="IXT21_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4867484.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(47291..47977)
                     /locus_tag="IXT21_000042"
     CDS             complement(47291..47977)
                     /locus_tag="IXT21_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4867485.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(47970..49004)
                     /locus_tag="IXT21_000043"
     CDS             complement(47970..49004)
                     /locus_tag="IXT21_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4867486.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(49399..49737)
                     /locus_tag="IXT21_000044"
     CDS             complement(49399..49737)
                     /locus_tag="IXT21_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ4867487.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(49739..50095)
                     /locus_tag="IXT21_000045"
     CDS             complement(49739..50095)
                     /locus_tag="IXT21_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ4867488.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(50101..51288)
                     /locus_tag="IXT21_000046"
     CDS             complement(50101..51288)
                     /locus_tag="IXT21_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ4867489.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(51479..52324)
                     /locus_tag="IXT21_000047"
     CDS             complement(51479..52324)
                     /locus_tag="IXT21_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4867490.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            52558..53523
                     /locus_tag="IXT21_000048"
     CDS             52558..53523
                     /locus_tag="IXT21_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ4867491.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            53900..54943
                     /locus_tag="IXT21_000049"
     CDS             53900..54943
                     /locus_tag="IXT21_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ4867492.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(55005..55469)
                     /locus_tag="IXT21_000050"
     CDS             complement(55005..55469)
                     /locus_tag="IXT21_000050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ4867493.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRT
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            55715..56662
                     /locus_tag="IXT21_000051"
     CDS             55715..56662
                     /locus_tag="IXT21_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4867494.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(56873..58945)
                     /locus_tag="IXT21_000052"
     CDS             complement(56873..58945)
                     /locus_tag="IXT21_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4867495.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(58878..59255)
                     /locus_tag="IXT21_000053"
     CDS             complement(58878..59255)
                     /locus_tag="IXT21_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ4867496.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(59384..59944)
                     /locus_tag="IXT21_000054"
     CDS             complement(59384..59944)
                     /locus_tag="IXT21_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4867497.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            60094..61386
                     /locus_tag="IXT21_000055"
     CDS             60094..61386
                     /locus_tag="IXT21_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4867498.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(61446..62564)
                     /locus_tag="IXT21_000056"
     CDS             complement(61446..62564)
                     /locus_tag="IXT21_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ4867499.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(62578..63999)
                     /gene="arcD"
                     /locus_tag="IXT21_000057"
     CDS             complement(62578..63999)
                     /gene="arcD"
                     /locus_tag="IXT21_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ4867500.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(64245..65366)
                     /gene="mnmA"
                     /locus_tag="IXT21_000058"
     CDS             complement(64245..65366)
                     /gene="mnmA"
                     /locus_tag="IXT21_000058"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ4867501.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            65770..66369
                     /locus_tag="IXT21_000059"
     CDS             65770..66369
                     /locus_tag="IXT21_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ4867502.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(66507..67793)
                     /gene="efmA"
                     /locus_tag="IXT21_000060"
     CDS             complement(66507..67793)
                     /gene="efmA"
                     /locus_tag="IXT21_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ4867503.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(68064..68408)
                     /locus_tag="IXT21_000061"
     CDS             complement(68064..68408)
                     /locus_tag="IXT21_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ4867504.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(68545..69681)
                     /locus_tag="IXT21_000062"
     CDS             complement(68545..69681)
                     /locus_tag="IXT21_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ4867505.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(69870..70844)
                     /locus_tag="IXT21_000063"
     CDS             complement(69870..70844)
                     /locus_tag="IXT21_000063"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ4867506.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(70924..71799)
                     /locus_tag="IXT21_000064"
     CDS             complement(70924..71799)
                     /locus_tag="IXT21_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ4867507.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            71929..73836
                     /locus_tag="IXT21_000065"
     CDS             71929..73836
                     /locus_tag="IXT21_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ4867508.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(74377..77655)
                     /locus_tag="IXT21_000066"
     CDS             complement(74377..77655)
                     /locus_tag="IXT21_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ4867509.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(77926..78471)
                     /locus_tag="IXT21_000067"
     CDS             complement(77926..78471)
                     /locus_tag="IXT21_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4867510.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(78483..79175)
                     /locus_tag="IXT21_000068"
     CDS             complement(78483..79175)
                     /locus_tag="IXT21_000068"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ4867511.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(79194..79478)
                     /locus_tag="IXT21_000069"
     CDS             complement(79194..79478)
                     /locus_tag="IXT21_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867512.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(79491..80054)
                     /locus_tag="IXT21_000070"
     CDS             complement(79491..80054)
                     /locus_tag="IXT21_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ4867513.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            80282..80944
                     /locus_tag="IXT21_000071"
     CDS             80282..80944
                     /locus_tag="IXT21_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ4867514.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            81068..82264
                     /locus_tag="IXT21_000072"
     CDS             81068..82264
                     /locus_tag="IXT21_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ4867515.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(82365..83741)
                     /locus_tag="IXT21_000073"
     CDS             complement(82365..83741)
                     /locus_tag="IXT21_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4867516.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(83778..84644)
                     /locus_tag="IXT21_000074"
     CDS             complement(83778..84644)
                     /locus_tag="IXT21_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ4867517.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(84733..85758)
                     /locus_tag="IXT21_000075"
     CDS             complement(84733..85758)
                     /locus_tag="IXT21_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4867518.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(85776..86450)
                     /locus_tag="IXT21_000076"
     CDS             complement(85776..86450)
                     /locus_tag="IXT21_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4867519.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(86634..87527)
                     /locus_tag="IXT21_000077"
     CDS             complement(86634..87527)
                     /locus_tag="IXT21_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4867520.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(87601..89298)
                     /locus_tag="IXT21_000078"
     CDS             complement(87601..89298)
                     /locus_tag="IXT21_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4867521.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(89291..90604)
                     /locus_tag="IXT21_000079"
     CDS             complement(89291..90604)
                     /locus_tag="IXT21_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4867522.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(90604..94122)
                     /locus_tag="IXT21_000080"
     CDS             complement(90604..94122)
                     /locus_tag="IXT21_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ4867523.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPECGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(94415..94873)
                     /locus_tag="IXT21_000081"
     CDS             complement(94415..94873)
                     /locus_tag="IXT21_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4867524.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(95171..95689)
                     /locus_tag="IXT21_000082"
     CDS             complement(95171..95689)
                     /locus_tag="IXT21_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ4867525.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(95691..96308)
                     /locus_tag="IXT21_000083"
     CDS             complement(95691..96308)
                     /locus_tag="IXT21_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ4867526.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(96305..97159)
                     /locus_tag="IXT21_000084"
     CDS             complement(96305..97159)
                     /locus_tag="IXT21_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4867527.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(97156..98067)
                     /locus_tag="IXT21_000085"
     CDS             complement(97156..98067)
                     /locus_tag="IXT21_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4867528.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(98108..99442)
                     /locus_tag="IXT21_000086"
     CDS             complement(98108..99442)
                     /locus_tag="IXT21_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4867529.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            99650..100480
                     /locus_tag="IXT21_000087"
     CDS             99650..100480
                     /locus_tag="IXT21_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ4867530.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(100724..100973)
                     /locus_tag="IXT21_000088"
                     /pseudo
     CDS             complement(100724..100973)
                     /locus_tag="IXT21_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(101164..101358)
                     /locus_tag="IXT21_000089"
     CDS             complement(101164..101358)
                     /locus_tag="IXT21_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867531.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(101863..102048)
                     /locus_tag="IXT21_000090"
     CDS             complement(101863..102048)
                     /locus_tag="IXT21_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867532.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(102898..103239)
                     /locus_tag="IXT21_000091"
     CDS             complement(102898..103239)
                     /locus_tag="IXT21_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4867533.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(103239..103469)
                     /locus_tag="IXT21_000092"
     CDS             complement(103239..103469)
                     /locus_tag="IXT21_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4867534.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            103756..104094
                     /locus_tag="IXT21_000093"
     CDS             103756..104094
                     /locus_tag="IXT21_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867535.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(104299..104538)
                     /locus_tag="IXT21_000094"
     CDS             complement(104299..104538)
                     /locus_tag="IXT21_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ4867536.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(104514..104861)
                     /locus_tag="IXT21_000095"
     CDS             complement(104514..104861)
                     /locus_tag="IXT21_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ4867537.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        31316 a        21680 c        18049 g        34139 t
ORIGIN      
        1 ggaactttga gatattcttt aggatattaa aattaactag caccacgggt atattctata
       61 tcttttttta ttggttttat tcccttgttc gttatagact aaaatccttc tttcagcatt
      121 attcaaatca aatggcaaca taatagagaa atttatagta tgtataatca tctgaattca
      181 atcaaatcaa ccggagtcat atacggagtc aaactatccc actgagagtt tattaaacgc
      241 agattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc aaaatagttc
      301 cgaccaaaga attataagga gtgaagacaa atgacctata cccatcttac aacaaatgaa
      361 ctcgtgatga tagaagccta tttcaatatg catcaatctg ttaccgtagt cgctaagacc
      421 tcaaatcgtt ccagacagac catctacaat gtgtacaact tcctgaaaca aggccaatct
      481 atccatgact attaccaaca atatcaaaaa aataaaacac ggtgcggccg acgacccatt
      541 tgtttgccag cagaacaaga tgcgtatatc cgaaacaagg tggcgcaagg atggacaccc
      601 gatgtgatta tcggtcgtgc tgagttttca atttcatgtt ctgttcgaac cctttatcgc
      661 aagtttaaaa caaactaatt tgattgctcg aaactaccca tgaaaggaaa acggaaaccc
      721 aacgggcata aagaacgtcg agggaaacaa gccttcaaac ggaatatttc agaacgtctc
      781 acggactatc ctgaatttca acaggaattt ggacatttgg aaggcgacac tattgtgggt
      841 gtccatcata aaagtgctgt catcacgctg gttgagcgct tgtcaaaagt gattattact
      901 ttaaaacccg aaggacggaa agcactagat attgaaaaag tgacaactga ctggctacaa
      961 aagcttcccc gaaacttgtt caaatccatt acctttgatt gcggaaaaga attttcgaat
     1021 tggaaaaaca tgagtaatgc taacgatatt tctatttatt ttgcagatcc tggaacgctg
     1081 tcacaacggg gattaaatga acattctaat ggattgttac gtaaagacgg acttcctaat
     1141 acaatggatt tcaatcaggt tgaccaaaaa tttgtggcgg cggttgcctc caaaagaaat
     1201 catataccaa ggaaatcttt aaattatcga acatcgttag aagtattttt gagttacata
     1261 agtgaggagc aattgtctaa cttaatttga catttagaaa ataataaacc atatcccatc
     1321 tattcaaata atattttacc gattgaaaga cttgctaatc aaagcagaat agtttatata
     1381 gataaattat tttctttaaa cttatactca tttttagata ttttataaga atacttcatg
     1441 tcttcgccag taatataaag aacatctgtc tctccatttg gacttgggtt atctattttg
     1501 ggtgtaacat actatacata caaccgatac ataaaagatt tgacatctgg acaaaacaca
     1561 atgctttact tcatttttat aggctttgtt tacaccatca caaaaatcac ctcctaattt
     1621 acaaattcat taaacaaaca gaaataaaaa atcaccacga tagccgcgtt caaaaaaatt
     1681 tacaaaagag aattttagtt aaagttttta gattcgcccc actaattacc aaaggtattt
     1741 tatttattct taatacgaaa taatttattg acatttagat taccaatggt atattattta
     1801 taaataaatg gaggtggatt atgtacacaa ttattcttgc aagccacaaa aatttagcga
     1861 aaggaatgaa agagacagtc gaatatatcc ttggccctgc accattcata aaagtaatga
     1921 ctgcatacac agacgataac tatgatattg aaaaagatac gaatattctt ctcgatggaa
     1981 ctgataaagt ccttgtcatc actgatttac ttggtggtag cgtaaataat cattggatga
     2041 attatgttta cgaaaaaaaa ttaacaaaaa aaattacagt aattgcaggt atgactttgt
     2101 ccttaattat ggaattaagt atgaacattg aagattataa attgagagaa aaaatttcca
     2161 tgattattgc tgaaagccaa aagagcatta taaactgttc agaattaatg gaggtagaag
     2221 ataatgatta aactattaag aattgacgat cgcctgatcc acggtcaagt tgcaatggct
     2281 tggactaagt ccgtatctgc tgactacatt attgtagttg atgacgcctc tgctaatgac
     2341 aaaatgaaac aaatgatttt atcattagca aaacctgtat caacgggact agatattatt
     2401 acttcagcag attttttaag tacctttgaa aaaaggaaaa atcaaaattt gatgatagtc
     2461 actagttccc ctaaaagcgc atttgaaatt gtaaacgcta tcccagggga agtcgattca
     2521 attaatatcg gtggtttacg gtacgccgag ggaaaagaga aagttaacga gtatgtggct
     2581 atcacatcag aagacaaaaa ctacttagca aaattaaagg agtccggctt caaattaatt
     2641 atgcaggcta ctccaactgg aaaagcgaag atttattaga aggtggggaa taagaatgat
     2701 tgaatgcatt ttaattggca tagttgggta tttaacgaca atagatgaaa gatattttgg
     2761 ggcatctatg atgaatcgac cgataatagt tggacctgtt gttggactta tcttagggga
     2821 tctccatcaa ggcatcttga taggagcagc attagaagca atgtttattg gcattgtaac
     2881 gattggtgct gcactaccac cagatgtagg tgtagctggt actattgcaa cggctctagc
     2941 aataaaaagt ggagcaggtg ctgatgtagc tgttacattg gcgatgcctt tcgctgtatt
     3001 ggcacaagga ttaaacatgt tggcttttac gattaactcc tttaccgttt cacaaggtag
     3061 aaaagcaatt gaaaaccgca aattatcatc ttttcagaag tggcattttt ttccattaat
     3121 cgttcgctta ccttccgcag tacttacatt tttagtagta tattttggtg ccaatgctac
     3181 agaaagtttt gtaaatatta tgccagaaaa attaatgacc ggtttcaccg tagcttctgg
     3241 tttactcccc gctgttggat ttgccatgct aattcaagga atgattgaca aaaaatcagt
     3301 tccttatttt ttcattggat accttttagc ggcatattta ggagtccccg tagttggtgt
     3361 agctgttgct gcaattatgg ttgctttatt agttatccaa actaaacgca atcctgtaaa
     3421 tatttaaagg gagagtgata attatgggag attctaaatt aactaaaaaa gaaataaaca
     3481 aggttttctg gcgttcattt gctttacagg gatccgagtc atacactgat atgcaaggaa
     3541 tgggatatag tttttctata ttacctgctt taaacaaaat atatcaaggg aatgatgaaa
     3601 aatattatga agctgtttac cgaaatacgg aattttttaa tacaacgcct catatttcaa
     3661 cctttattat gggtatgtca ttagcacttg aagaagaaaa tgcaaaaaca gatcaatttg
     3721 aaactgaaac aatttcatcg ataaaagctg cattgatggg gcctgtagct ggtattggtg
     3781 atagttttta ctggggaaca tttcgcgtta ttgctgctgg catcggctta tctttagctg
     3841 cccaaggaaa cattttaggt ccaatattgt atttttcaat ttatacgctg ttacattttc
     3901 ttggcagata tggctgctta cgtattggat atcggatggg agcaaacgcc attgaaaaag
     3961 catttgagaa caattacctt gaaagtttct cgttagcagc atctatcgta ggtttaatgt
     4021 caattggagc catgacctca tctctggtac gctttactat tcccgccgta tttaaatcaa
     4081 aaggtggaga aataaatgtt cagcaaatgc tcgatgatat ttttccagga attctctcat
     4141 tggggctaac attctttgtt tactttctat taaagaaaaa agtaaaggtt ctttggatta
     4201 tacttgctat cttcgtcttt agtattgttg gagtttatat tggattatta ggttaattaa
     4261 tccagaataa aactagatca gacatcaact taaaccataa aatacccaaa gataaatgta
     4321 aatatgagta aaaagagcat ggtataaatt attaggatct ataatttatg ggtctgatga
     4381 atcagaattg attctgtagt gacctaaaaa agatagatgt tttcggagtg gagaaaattc
     4441 actccgtttt tctttgcttt tcttgatgat tttagacagc tttgaatgct attactctaa
     4501 attgcaccgg actctgccag tttagtttcc ttttcatacg ctcatggttg tagtaatgaa
     4561 tatagccatc aatggcttgc ttcaattctt caaagatacg atagtctctt ccataataaa
     4621 tttcctgctt taatagacta aggaagttct caatgatcga attatctaag caggtctcct
     4681 tacgggacat actttggaag atcttatgtt ctttcaactt atgactataa gctttcattt
     4741 ggtaggtcca tccttattgg agtgaaaagt tcgacgatag atacattccg ttgcgcagat
     4801 agcttcctct aaagcttcca tgatagccaa cgcacttggc ttctcagaaa tgcgataaaa
     4861 taaaatttca ctgttgtaca tatctaaaaa tggatcaaga tacagcttct tttcacgatt
     4921 ggcaccgtat ttatccattt caaaatattt tagttctgtc gtatctgttg taaccttttg
     4981 atgacagttg cttgtgtaaa agcgtcgatg aattcgattt tttgtaatca tgccgattct
     5041 tccacgataa gtattatagc ggcgatattt tctcgtaaat gatcgtactt caatactcag
     5101 ctttcgaatc aaacgttgta cttttttctt gttaacatgg aacccacggt cccacaattc
     5161 ctttgtcata cgtaaatatc cataatcttt atattgactt cttattttaa gcatttcctc
     5221 ttcgatttct tgatctgaat ttcttcgatc aaatcgtctt taccaataca taaaggttaa
     5281 ttcaggaaat ttcaaggttt caagaagctc aactaatttg aatgatcctc ggaggttgtc
     5341 gatgattcgt gcgattgatt cgttcgtctt tgttagactt cctgttttct aagcttcctc
     5401 aattctttta aaaaggtatt ctcaatttga agggatcgga cttgcttttc taatgccttg
     5461 atacgatcgc gttcttcagt tgttgtattt tcttttttgt tttctttttt tctagtcaaa
     5521 gtaagtggtc gccctttctc ttttgagagc ccctcaatac ctgcttctct gaaattacgc
     5581 atccaattgg caataagcgc agggttgttc atttctaaag tgctggcaac ttctcgatag
     5641 gacatctcac ttgtttgata taactctagc gcatttaact taaattgaac agaatatttc
     5701 tgattttgat gctttttcaa tagaccttcc tctccgtatt cacgataggc attcacccaa
     5761 tcacttactt gagacttgct tgaaataccg tatttgttgg caagatgccg atattcgccc
     5821 acgccactaa gatattcttt aacgattttt agttttaatt caaaaggata ttttgccata
     5881 caaataacga ccccaaaaag ttagattttt tggtctaact tttgattgat ccatccgtgt
     5941 accttttttt gatctcactc cttgtatttt tgctaaatat cctaatccac cttctccttc
     6001 aagatactct tgaacgattg ttgacttgaa ttcaaagcta tattttccca taaaaatacc
     6061 atcccccctg aagttagatt ttggtctaac tttttagggt ttggacatca ccagtcccat
     6121 tttcattcac gtaatgcaag aaactgactg aagtcattaa acaaaaatct gagctagaat
     6181 gaagttcatt ctgactcaga tttgattttg cacaactcat cagtcttcgt aaaatatatt
     6241 ttgcctaata agtttctttt aaagtatcta taaaacacaa atttttttga ataacatatt
     6301 ccttccggaa tttatttcgt tattgtgtga tttctcagag tacaatggat gtatggaaag
     6361 gattcaacga gaatttgcta atcagtcctg tagctttgtc taccttcata agtctgttat
     6421 ccaaagcgct gtgtttacta tcaactcatt agctctttta ttaccgtatt ctattaggcg
     6481 aggtcgtgac cacacagtga gtttctgtgg aatcccacag tatcgaagca ttttctagaa
     6541 tttatatcga aaataggttt ttattccatg aaattatttg ttggtaatga cgttagttct
     6601 gaaaaattag acgtttgctt cctaacagac gacgatcagc tatctatatt gactgaaaat
     6661 tctgttgcta atgacattga aagggctcca ttgacacgag aactgattct tgtgttcaat
     6721 gaaaacaatc acttcggtca aatcgttatt gagatggaat taaacgtcca tgtacagctt
     6781 tcatccttcg atgtttttcc atgaagatgc agagctaaaa aaactcaatg cacttgtgag
     6841 gattgaaaat ccttttcgga tgaaacaatt cagtcgcatg tttgaggaag atacgactga
     6901 tcgaaacaac gcgttaagaa ttgctgattt attgcgtatt caacggttca caacttctcc
     6961 tatcaaagaa gagaagtacg tggttttaca gcgtttaaca cgcactcgct atcgactcat
     7021 taagcaactt cactcgtaca aaacaacact ttctggaaaa tctaacgtac aaatgcaata
     7081 cgcttactcg ataaatgcgc gatgaatcga caagtctcta tagtgccact gtcattactc
     7141 ttgtgacaga agacttcacg ctagatgaat tggctgtgct tccttttgaa gctttctgtg
     7201 acttactaaa agaaaaagga aatgtcgctt taaagatcca gaaaaaaatg ccaaatcgat
     7261 tcaacgtgca attgccgtga gttatcggtt agatagtctt gcataagaat ccatcaatgt
     7321 cgttctaaat gttcttgtat gtgaaattcg tgcgcttgaa aagaacatca aagacttgga
     7381 taaagcaacc gagcaatttg ttgtcgttat tccagaatat caatatttga ctagtgttcc
     7441 aggtattgga aaggtacacg ccgctggatt aatcgctgaa atcggtcaaa ttgaacgatt
     7501 tgaagatcaa acaaaattgg caaaatacgc aggcttatgt tggaaaggca aacaatctgt
     7561 taactatcgg tcacaaaata cacctctgac aaaacaaagg agtcgctatt ttagatacta
     7621 cttagttgaa gcttccaact ctgtaaaaaa atatcttcct caatataaag ctttttatca
     7681 aagcaaatgt aaagaggtcc caaaaataaa cacaaacgag cacacgtctt aaccgcaaga
     7741 aaatttacgc gtctggtaga tacgctacta cgtaaccatc aactctacac gccaccaagg
     7801 aacgtgatag ataaataaca ttctgttatt gacgcaaacc cttgatatac cccgaaaaaa
     7861 tttgattttg gccggggcta gttaagtacg cttcaaaaca ggttacacaa taaaaatatt
     7921 ctcaatctcc aacttgtctt attaccatta gacttattga caaagagaca attaccatgc
     7981 tcttttttac tatttagtct actatatatt ggtttagaca gccttccaag tcttatttca
     8041 tcacgcagcc taccaagtcc tttaaaaata aattgaagct cgcaataatt tcagcaaata
     8101 atcaacagca atatttttgg gtcgtacata acatgaagtg cttgtaccta ctagtctcgt
     8161 taagtaacag aagattatct ttgcttgact aatcaataaa ttaagtaaat aacaatataa
     8221 tattattgag atatttattc atactatcaa tgacgcttta acaacgatat gctccctcat
     8281 gcaattagcc agtgttattt tcatcatagc ctcttgcctt ttcacataca aaaatatagt
     8341 tatgatgagt ctactataca aattccagca ataaaagccc aaagcacaaa gctaagggtt
     8401 aataggtgta gcatgaccaa aggagccatc aatcatcaat gtttcatatg caaggtttgc
     8461 acctttgtga aaacaatcaa taacatgttc tatcattgat ttttcacgtt tagataagac
     8521 aaaattactt gcaaaagctg caaagtatgc atcacctgcg cccattgtat catgaggtga
     8581 ctcaactttg cgaatgaatc cttcataagt cttattctct cgagtgtcaa caaaaacagg
     8641 gctcttacta ccacgcgtaa aaagaagata cggaacctca aagtccttgc attcactaat
     8701 taagcgatta atttcctctt gatcatctgt agcgactgaa aactgtgcaa tataaattga
     8761 cggacaaaca tctgacaagt attcatgcgt tctatattta tctaagtcag aaaaatcaaa
     8821 agaaacttta acatcatatt tttttaattc tttaattttt gtttctgttc gactatggca
     8881 actagtatga actaatgaac tgttcttcat caaatcaata agttctgctg agagagcagg
     8941 tgtcaatcgg gagccacgca cgccacctat aaactcccta tctccatctt taatagttac
     9001 atgtgcttgc tcagtaggct cgtcaataac ttccgagaaa gagatatcaa caccttcaga
     9061 actgatggcg tgttgtaata acacgccatc aggatcagat gatagactac cgacatagta
     9121 tgctttaaca ccactctttt ttgcattcac agcaaaatta actgcatttc cccctggaaa
     9181 tttttgattc aaattctcat agtaatcaac aacattatcg ccaaatccaa tcacatacgc
     9241 tgtcataatt aatcacctac ttttagtatt caactactcg gtaataacga cgtttatcca
     9301 aagagtggtt ccgaatatct tcaagatgaa cactgacgcg acctagggct gcccacataa
     9361 ctggagcaca aaagtacttt cgaaattcgt cactaatacc tggtagctca taatccttag
     9421 tatcaaaaac attaaattta tctgtatatt ggaccgcaaa tttttcaact cgagcatcta
     9481 aaagccgtgt ttccccctca gttttcaaaa gtgttacaca aactcctttt tcgactaact
     9541 cgagggtacc atggaaaaac tctggtgatg agacagactt tgtacgcaac cattgagatt
     9601 cctcaagcac acacattgaa taagagtagg tgattcccca taaattcccg ctaccaatcc
     9661 aaatttgata aggatcttct ttaaattctt cggcatactt cttccctttt tcatcagcct
     9721 gttctctaac tgaattcaga gccgttggta agtttttcag ttcatcagca aaacgagtgt
     9781 attcaggaaa ttccccattc aaattcatga tagacataat taagaaataa agtggcattt
     9841 cttgagggcg gccgtctagg taattgattg cataagttga tagagcttct aacggtgtat
     9901 cttctttact tacaatacca acggttggca cgtttcgttc ttttaaatat tcagctgcct
     9961 taatagtttc tttcgtatct ccagattttg agtataagat tactaaagat ttttcattca
    10021 actgtttatg tcccattaag acaagctcag ctgcagtcat agagtaaaca ggaatacttg
    10081 attcagtcat caatacatac tcaaatggtt gcattacggc taccgatcca ccagaagcag
    10141 taaagaaaat attactaatt ccagaatcat aagcttgctt tgcaattttc tctacatctt
    10201 cacgaatacc gtaaatccgt ttgccttttt ttttcatttc ttttgcatta tagtttaaca
    10261 ttagttctct ccttacaatt atttagatta ttcaattaag ttgtcaaaaa tccgaagaag
    10321 gctgctatga taccaaagat aaagattccg aataacacgt aagttgtttt aaagcctttt
    10381 ttaagcatcc agtaaatcag aaatacacat aatactgaca gcagatttgg taaaatacca
    10441 tcaatgatac tttgaatttt tgttgcttca tcaccttgtc caatagaacc agctactttg
    10501 attgatacca tacttccaga catgccccca attaccataa gacccaaaat tgaggcagca
    10561 tgagtcaacg ttttcatgac gccagaggat tcaatgttct ctaatagctt tgtaccaaag
    10621 ttatatccct gcataactcc aaagtaacgg agaagaaaat tagggacatt gaagattagt
    10681 aaaaataaaa atggacccaa aatattgcct tcttttgcta atgaagcacc tacaccgata
    10741 ccgataacta ataaggtacc ccaaaacatt gaatcaccaa taccagctaa tggtcccatc
    10801 aatgcagttt taatagaagt gattgtagaa gtatcaaagt catcaggatt tttggcattt
    10861 tcttcttcca tagaagccac aattcccatt atgaatgttg ataatggcgg agctgtgtta
    10921 aaaaactcta gatgtctttg agctgctttt tgcttttctt cttcattgcc actataaatt
    10981 ttttctaacg ctggcatcat agcaaaggca aagcccatat gttgttggcg ctcaaaattc
    11041 catgatgctt ctaatgcaca tgaccgaata caaatttttg ttaaatcatc tttagttaaa
    11101 gctttcccac tagaagtcat cttcaaccat ctcctcccct cttgaaccca ttgcagcttt
    11161 ggtttccatg tttactataa tataagctag acatgcgccg aagattgcaa tccccagaat
    11221 cggcacatgt aagtagctaa agagtgcaaa accaattacg aaatatacaa agacatcttt
    11281 cttaagaagc atttgcaata aaatagcaaa acctagtgct ggcataatgc ctgcagcaat
    11341 gttaatccca tcttggacaa aagttggaat agcatttaag attcgttcca taacaggact
    11401 tcctaagtaa aatgagaatc cgacaaataa agcccgtggt aaacttttta ctaagacaaa
    11461 acctaaaaca tgcattttcg acacattttt tagttcgccc ttttcagcat atctatctgc
    11521 ctttctagca atcattggta gaacaagcaa tgttagtaga ttatcaacta ataagaatag
    11581 cgcagcgatt gggaatgcca aggtcaacgc aaattctgct cccttgccag ttgcaatagc
    11641 aaacgcagtc cctaaaatcc caccagatac cgtatcaggc ggacgtgccg ctccgattgt
    11701 tattgctccg ataaatgcta actccagtgt tgcccccatc ataatcccca tcttaagatc
    11761 gcccataatt aaaccaacaa agacggaaga tacgattggt cttgataagt tattcgtacc
    11821 tgtcatccag tcaattacga tgaacgcagt caagagacca agcaaaattg cttgtactaa
    11881 gagactatcc atgaaattac ccccttattt ttttattaca aagatttttt taaatctatt
    11941 gccttatctg ttggaacttg ttgagcgaac acttcaatgc cctctgagac cattccttta
    12001 agttctgctc gttcctcttc tgttacaaaa attgtatgcg tcaaagcgct actgccacta
    12061 gtttccttaa cactaccgat atttacagat ttaatttgtt caaccccatt tgccaaccgt
    12121 ttagcatcgg aaatggtttt ggtaactaca aatagtttgt atttatctgt cttcccacta
    12181 ttaattgcat caatcgaatc ttgaattgat ttgataacaa ccttcacgtt atgtggtgcg
    12241 gctaatttca aagttgtctt ttgaattgga tcattcaaaa tttcatcgtt cgctactaaa
    12301 atgcaatccg cactgattgc gttcgtccaa gttaaagcaa cttgaccgtg tatcaaacga
    12361 tgatcgatcc tcactaattt aatcattctc ttccctcctt tatttaaaag tcatcaaccg
    12421 tttgctcata atctagctga ttacaaaact taatcgagcc agaagatgct tgaattgcat
    12481 gatcaataat ttcttcaatc tgatcagatg aaactaattg gaattttaaa tataaatcca
    12541 ctaaaagact taaattcatt ccagaaatca aatagacttt agaaagatct ggcaaccgtc
    12601 ttagccattc attattaatg cttccaccaa aaatgtcggt tacaacaatc gtctcatttc
    12661 ccatagattc ctttagataa tcatcgatga tcatttctac gtcattattt ccatcgacat
    12721 aggcacatcg aaaatcaata ttatccaaat ctcctacgat aatactcaaa gtttcaatca
    12781 ttccttttgc tagaggaccg tggctcatac ataatatttg tctcataccc gaactccttt
    12841 cgctttgtta atagcaaata ctgtgccaat tactgtcacc acttaaatga aagggcgtac
    12901 aaaaaataaa gtgacactat ttccatcgct gtgaatagtg tcactttatt ttatcaataa
    12961 cttacaatga tgttgcacac atatgcaatc tcttccatag gaatcttaat attataaagt
    13021 ttctcaatct tactaagtgc cttttgaata gaagcaacct cagtttcgta tcgcttaaga
    13081 aactgatcta ttgctggata actacttact ccctgattac gaattagtcg ctcaatcata
    13141 cagctgatat gaacataaag attaattttg gacaatgatg ataacgtttt ccccaccctc
    13201 atttgataat cttttaaact ttcctcaatt tgattgacaa ctttttcggc atctaaaata
    13261 gtaagactgt caatcaagcg gtttaatgag ctatttttaa caatattttg attaattgcc
    13321 tcagcaatct cttttccata gactctcaaa agaactttat agagtttgcc aatttcacca
    13381 tcaaataatt cttcaagaga aatataggga atgttcgaca cacgcggatc atctgttccg
    13441 ataatggcaa tgatatcctt tgttttaatg agaaactcta attgctgagt atctctaaga
    13501 gtttcatttt cgtatggcag aaattcaatg cccaagtctt tcgacaaatt attttccatc
    13561 atttctttaa tttttatgga ggtacccaaa ccagtattgc aggaaacaac gattgtacgt
    13621 tttctaattt tgatgggata tagaacttgc ttttcaaact gagaatacgg caattttttt
    13681 acaacctttt ggatatcact cgtactttga atcagttgtg cggtttcaat agccatttcg
    13741 gttgtaacat tattaataat cattgttgga acttcgatta cttgttcaat ttcagtctta
    13801 atttgagcta aacttcccat atcaacgaaa atcactagtc catctttagg ctttcgatac
    13861 tctatatagt gaagtaatct gtcaattact tcactaaagc tgatatctag aggcatatca
    13921 atagaatcta tgacaaactg attaaccaaa cgattggcaa cattagcaat actactagct
    13981 gtcgcatatc catgggctaa aataattgcg ttaatgtctt tcgcttcatt tccggtctca
    14041 ctaataacac acaaactgat atacagttta tcccaaatag tcaatgttgt atccagggcg
    14101 ttctcgaaga tcagtaagaa tacatttaaa tccgtcaaat agttatactt taggttttca
    14161 tataactttt caatttcatt cgctgaagca ttctctttcc cataccccca ttgatcccta
    14221 atagcgagat aggtagctat agcttggacc gtattgccag aaaggtgaac aaaaagactc
    14281 ggagagattt tttgaacaat attttggatt tccggtaaat aatacttaac taagacttct
    14341 gaattttttt ggtaaataaa acgatcatta aattctgcca tttggtcctt cacaaaacgt
    14401 cgaaactctt cactatttct attcacatca tgacactctg aataatattt agaaacacga
    14461 tgaatcaaac ctcgtaaatt tgtagttaca gttgaagtct cgtcacaatc atgaacatag
    14521 ttaccattac taaagatata gaaccgttgg ctcgcaattt ggttttttaa ctcaatattt
    14581 tctgttagca agtacctgct aggtaaatca gaaatatgaa tttcgatata ttttttattg
    14641 cttccactaa atagtttatt cgcaatagtt tgttttatta gtccatccag ctcaccaatg
    14701 tttccataca aatctaagcg aatcaggaaa ctaagggcgg cttcagaaac taatatttct
    14761 ttattgatca gagtagactc tttctctaat aaatggaaga caatctcttt tcgttcgcta
    14821 atttctctat cttggtaatt aggcaaagta caaacaatcg gaattcttcg tcgaaaagtt
    14881 tctagaaaaa aattttcttc ctgttccgtt gtcgcaaaaa tatatctaac atgtgataaa
    14941 cgccaaggac cactatctcc aactcttcga aaaacgcctt tatccatatg aataaaaagt
    15001 ttctcttgtc cttctggtgg taaacgatga atttcgtcaa gaaagacgat tccaccgtct
    15061 gctgcttcta ttataccaat atggtcagaa tcggcgccgg taaacgatcc cttcatatgg
    15121 ccaaacaact gacttgttaa taactctgga ttattaaaat actgagcgca gtttaactcg
    15181 atgaacggag cattctcatg aataatacca gtttgtcggc aatactcagc tagtttttcc
    15241 actaagtaac tcttcccaac tccggacgat ccaaaataca ttattggtaa tccacctgga
    15301 tatttcattg ctgatatccc ttgttgaacc gcttctttta aactttgttt tgcaccaatt
    15361 aaatcattaa aaaaatgatc tgcttttttc tttgaaattt tttctctaaa cgaatcaatt
    15421 gaagaaaact caaaactatc taatgaatgt gatatatacc gttcaattgt atggactgga
    15481 aaaaaaagaa caggacgacc attgattttc acaacttttt tctgttccat tagttcgttt
    15541 agatgcttac tagccgtatt tctttttatt tgaaattttt gggcaatata ttgcgcgtta
    15601 cattcttctg gaaaatattg tggaaaatca tagctgttaa gtagatcatt taattctttt
    15661 aaaattttcg ttttcaaatg aacccacctc ctttacttaa taataaatga ttttcactat
    15721 aaaattcaat attctttata aacatctttc cactttaaca atacctatgg tattttttct
    15781 aaaaaatatt tgacactttt tattacctgt ggtattttat ttatgcaaac aaaatattac
    15841 cattggtaat caaaggagag atttttatgt ttaattttga tcaagaaaga tttttaaaga
    15901 ctcaagctgg tgccgttgca acaaatgaac aaatttccaa tattttagaa tccatcgcat
    15961 ctgacttaga agaaatttac tttattgggt ctggaggagt tggtattctt tttgattcaa
    16021 caatcgaatt cgcgagaact cgtagcactt tcccttttca tcgtgttatc gctgccgaat
    16081 ttatgattca tcccaataaa acattttcta acaaatcttt ggttgttatc ccatctttat
    16141 ctggtacaac aaaagaaaca cttgagttag tcgaatactg taatgaaatg aacgtcaaaa
    16201 cattagcttt ggtaggaagt cctaaaacgc ccctagaaaa agctgcaaca tattctatct
    16261 ataacgacgt tttagatgat acatcttcag aaaattatta ccttcaagga ctgctgattg
    16321 ctctcttctt aatgaaacaa agaaatgaaa tcgatatcaa tcaatacaat acctatattg
    16381 aggatttaaa aaaaatacct gaagccttag tgcacgctaa agagcaagcg gaaccaatgg
    16441 caaaatcttt tgcaaaaaaa cacaaagata cttcatatca tattttaacc ggtgctggtg
    16501 atagctttgc ggaagcatat tactatggca tgtgtatttt agaagagatg caatggatta
    16561 agactcgtcc tgttcatgca gcagacttct tccatggaac tcttgaatta attgaagcgg
    16621 gtgtaagtgt catcattaca gaaggagaag atgattctcg acctctcaca gaacgcgtga
    16681 agaatttcgt tcctaagttt acagatgttc acgtaacggt tgatacaaag gactatgact
    16741 tacctggcat ttcggaagac acgcgaaaaa ttatttcccc agtcgtctta gctactgtct
    16801 ttgaacgaat tagtgctttt ctagaagaag aacgtaacca ccctttaact actcgccgtt
    16861 attataagca aatggaatac taatcataaa aatatgaaaa aggtgaccgc gactccattg
    16921 cccatttgat tgcttcattt tctctaatgg gagaatagaa tcgctgtcac cttttttagg
    16981 aggatctaaa atgaaaatcg cagttatctg tagtggtggc gactctcccg gaatgaattc
    17041 agctttatta gcaataaaaa aaacaattga tagcgaacca gattcggaag tagtcttttt
    17101 taagaatggt tatgccggaa tatttaagga atgtattgcc tcagtttctc taaaagaagt
    17161 tgaggtattc aaagctggaa cacttattgg aacagccaga tttgagaaaa tgagagatct
    17221 cgaaagtcgg aaaattatta ttgaaaacct aaataagatg gagatcgaca acttaattgt
    17281 tattggaggt aacggttcct tgaaaggagc tgaactattg aaaaatgaat cagaattaca
    17341 agtatctgtc atcccaggga ctattgataa cgatttcccc aataccgatt atacacttgg
    17401 cttcgatagc gccttaaaca ctataatccg atcaatcgat gatttaactt ctactgctaa
    17461 tgctcatgat cgtattatta taatggaagt catgggccga ggctgctttg ctcttccaga
    17521 gtacgcttct attgcaaata atacttattg ggttgagaga gacgacaagg aatacttaca
    17581 cttaatatgt aaaaaatttg agtcggagtt ctactcgcct atattgcttt taccagaagg
    17641 tatatctaac aaacaaaaaa tcattgaatt tattaccaaa aatttaccaa aagaaaccaa
    17701 atacttaaat cttgcgtatc ttcagcgtgg atcagctcca actttttatg accgatatat
    17761 tggctatttg tggggtcaat tagcggcgaa ggaattaatt gaaggaaaat ctgggatatt
    17821 aagtcaaaaa aatggtaata taatgttaat accttggtaa aaggatggat tactatgatt
    17881 tttgacaaag taaaagaaca aattcgaaaa gtgtatatca ccactgatcg gaaatctgaa
    17941 ttattgcctt cggaaagaga tttggcccag gaattttcgg tgagtcgtcc aacaattcgg
    18001 aaagctttag cttctttaga agacgaagga attatttcta aaaatgaagg tcgcgggtat
    18061 ctagtgaaac cagcttcaga aaaatatgtt gatcacgaac tcgactcttt tatcggattc
    18121 ttccaagatg cggttcagca aggaaagaaa atcagttcta agattatcca acaaaccatc
    18181 atacctgctg attcaacaat tagtgaatgt ttggagattg agcagaatac tttagtgctt
    18241 gtcttggaaa gaattcgata catcgatgac tatccaactt gtattgcaaa aagttgcatt
    18301 cccctatctg ttttacctga aattgttgaa catgattttg ccaaagaatc cctgttctca
    18361 gtattagaaa gtaggggaat tagattagct tatgccaaac gctcaattga agttacaccg
    18421 ccaacagatt tagaaaacct ttatcttgag ttagaaaaaa atgagccagt ctttgtcttt
    18481 acaagtattg gatttgctaa aaatcagaaa ccgtttgaat atgaaatatc taagtaccct
    18541 gcttataaga caaagtttga aagtactgtt tccaaataat ttttaaatat gcttaaatgt
    18601 ataaagagcc tcgcggataa cagataaagt gtctttccgc aaggctcttt ttctacttcc
    18661 tctttatctt aatccgctaa gtagcctaca gtccaaacat tatcatctaa ttaaggtgtg
    18721 tggaggtact cctctaccct aagtcaggat agtcgacata gttgttaata agtcttcaaa
    18781 gcaagcctga acctttttca ttaggtgtgc ttacttcaca tatgacgcta tcaaaaatga
    18841 tagcacccgt tgaatcaact aattttctct aattcatatc cccattcaaa caactatatt
    18901 ttaaaaactt ttttaatctt tgattatgtg tagcaaattt gtctaatttt tatttctttg
    18961 caaaattttt ccaaatttac cagataaaag taattcatat tcatgattga gtcctttacc
    19021 aataaaacct atttcgcaaa aattatttgt aagcattaag gattttctgc tgactttcac
    19081 tggaaggata acccaagaat tctgaccaaa gtgaaagatt aagaaccatc tttgccagtc
    19141 tatctatgtg ctattgtaac tgtcaacatc atgcgggcag ttattattat ttatttgaat
    19201 aatattttta ttataaatca gttttttaga ttgtgcataa tcactaattg ctgtgattaa
    19261 ttccagaata tggctctctg ggaaaatgtg cgcgtcatgt attcaatttt aaattctaat
    19321 ttgtacaaaa tgaatcgtca cgactcgatc tgacgcaact ccaaatgggg gcgccgacct
    19381 gtaccaagta cagtggcatg ttctttaaac atttgaagca aaatctgtcc atcaaaaacc
    19441 tgtattctca aagtgaacaa ggtgcgctaa atcaggaaat acttacactg atcatttctt
    19501 aatttaccta cttaactaaa atttaaatca atatgaaagc gacacttttc aaactaaaga
    19561 ggtcttttca ccgcttgatg tttgcttcga taaaaaagtt gatgtcggat tagttttcat
    19621 tttaataaaa cgtactattc ctgaaaaaaa tttatccact actaagtatt cggatttttc
    19681 taatttttaa caaaatttat ttcgaaattt tctgatatta tattcgcaac attagtgaaa
    19741 attcttagat aaattcattc tttcaatctc cgacttttag atatatgtga aatatctgtt
    19801 ttttgaatgt ttgcataagt taaaagatga gcgtgcttta gtattcttta gaatagcttc
    19861 ttatacaggg gaacgaaaag gagaaatact cggactgaca tgtagagaca tagatttcga
    19921 taatcaaact atctgcttca acaaaacgtt agtagaacta tccgagggct ctctatgtat
    19981 taatcctaca aaaacagaca aatcaaaggg agtaatttca attgataatg gaaccttacg
    20041 tattctaaaa aatggaaacg ttctgtaaca taaaagaacc tttctagagg gattcggata
    20101 gataatatag atgatatacc tgtaatcagc actatcaaag ggaataaaaa aagggacttc
    20161 ctttacaaag gctaccctaa ccatgtcatg gataaagtag cacggaagtt cccagatatg
    20221 aagataatca aagtccatgg cttcagaaag actcatgcta cccttctctt tgaagcaggt
    20281 gctcaagtaa aagatgtaca agataggcta ggacattctt cttctaaaac aatcatggac
    20341 atttatatta aagtatcaaa aaatcggcaa aatgaaacag tcgaattatt tgattcttac
    20401 atgaacggat gataagcgag gtcaaaaacg gagtcagagg atattatggg gatacctttt
    20461 tacctcttaa tcctttaata tcaatccttt aagcttcgac ttttgcaatc ttcccttgtt
    20521 cgatatagac tcaaatcact atatcactag atatcaaacg gcgtcatacg gagtcaaaaa
    20581 catcaaattt gacctgttaa atggtcattg aataacattt aaaatcaata aaacggagtc
    20641 agacacggag tcaaaaacgc tgagagtaaa aaaatatata cagccctata ggtaaagtac
    20701 cgcagggctg tatatttttt atttcagatt acctgaaatg attccattta atttttatct
    20761 cattttttcc tactagttag tatcaaatac tcccctattt tctcaataaa ctattttgga
    20821 ttacatggta tctgaaacag caaaaacctt gatattacag taaaaaatca cgttattttc
    20881 gtttcatttc agatacattt cacttaagcg gattccaaaa aaacaaaaga aaaaggaacc
    20941 cctagttagc ctatgctttt tattggttcc tctcaaaaca cggtcatata acaacggggt
    21001 ggggtaaatg aatgaaaaag gtggaggaaa cattattcaa tagctgtctt aaatacttcg
    21061 ttataaaatg tgaacaaata aaagacatct atccctattt tctacacttc cacttgacta
    21121 ccatctttga atcgtacaat tttcttaact cttctcgttt acctcaattc attctcaagt
    21181 ggaatcaact catcatctaa cattttacgc catgctcgct tcatttcttg tgggtattta
    21241 aatggcttcg cttctggatg tttggcttta tatccttcgt aatccttact gttgataatt
    21301 gctttcatat taggaattgg atcttcacct ggtagatatt gcagattaga agcatgtaat
    21361 tctttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc actcgcttgt
    21421 gtaaaatagc gacgtttaat gacaaagata tcttcattac tatctaaagt ccccacatca
    21481 aatgcattta aaatttgtcg gtaaacttct ttgattcctt ctgttttatt caatttagcc
    21541 aaagcttttt caatttcttc tcgtgcatct gaactattcg cagcataagt tcctagtaat
    21601 tgatcaatat aagttgaatc aatatcatac aacttagtgg tattttcact ataaaattcg
    21661 atcgttgaaa aatcttctcc ttctttttct ggttcttcct ctattaatga acccttcacg
    21721 gtctcgtaca cgccaatttg ttcttccaaa agttgaattt gattgttcaa agttggagat
    21781 ttcaccatgt catcattgta atcgtcataa gtcactaacg attcataagc acgattcaat
    21841 tcttgaaatg ctttgacgta ctcgatacgg atttcaagtg gcgtatgttc atcaatggat
    21901 tccatggatg aaacctcttt taacttacca tatgcttgtt tgaatctttt tttggattcc
    21961 tgataacttg gataaatcaa acctgattct tcttttgctt ttgaatacag ttttgtcgca
    22021 tcctcgacat tcttctccat tgtcgctggc ttacgaaacg tcacaatcaa tccttttgtc
    22081 ttatcaggat acgtacgatt cgtgcgtgaa aaagcttgga tcaatcctgc atactctaga
    22141 ttacgatcca caaatagggt ttgaatcgta ggggcgtcaa aacctgttaa tagacgatcc
    22201 actacaatcg ctaaatcgat ttgtttccca aattgtttga attccgctct ttttcgtgcc
    22261 aaacgattat tgatgtctcc gttataacgc tcaatgtctg ctaatgacca ggaagtatca
    22321 taatactgat tatattcctc aataattttt tgcatttctt cttgttgggc acttgaatct
    22381 cggctatttt cttctaatga ataggtaata gcgattcgtg gaaaatcagg atcctccatc
    22441 gttcttcctt cacgaatggg ctgattagga aactcttttg ttaaccagtc aggttctttc
    22501 gtcatttctt tgatcgctcg ataatacttt ttggccatgt caatggaact cgtggttaag
    22561 attgcagatt tcgttggacg gccatttcga aaatcaaatt ttgtataggc attatctgga
    22621 cggaacattt tatgaatgac tttttggata tgttcatctg cttcaaaaac agtagggtcc
    22681 aaataacttt cttttttgac aggctccatt tgatcgatca tacgattgat ctgttctgat
    22741 gaatactctg catatgtttc tacttctttt aatttccgat agattttatt ctctaatgaa
    22801 gtcggctcaa cggtgttttc atgctctact tgaaatccta agacagagcc atcttctagt
    22861 gcattcttga tggtataggt gtgcaagact tctccatact gatcatgggt cgtacgtgcc
    22921 aattgccctt tggcttgttt ccgattctca gggaaaatcg gtgtacctgt aaagccaaac
    22981 caagtggact tcggaaacat ttttttgatt tcttccatat tttcagcact taacgctcga
    23041 tgacactcat ctactacaaa cacgatatgt tgccccatta atttttggaa gcgattggtt
    23101 ccttttttct cttcttgttt ctttgcatat ttcaacgccg catctagctt ttgacgggtt
    23161 gtaataatca caacatttgc attggcatct gccaacaggg tttcacttaa ttctttggca
    23221 cttcctgttc ccacgatcaa gctattcgct ttggcgttcc cagaagaaat ccccgtatta
    23281 aattccgaag caaatttcgt aaactctgtg gtggtctgat tatctaaatc tttgcggtcc
    23341 actaacatga tcgttcgatc gatccccgat tttcgcgcta aaagcttagt agatacaaaa
    23401 ctagtcaacg tctttcctga accagtcgca tgccacacat accctgattg atgtttattt
    23461 gcggcaataa acaaggcttg aatcgcatga acttgataag gatgtaacac catgagggtt
    23521 ttattgtctt gatcttcact gacaatcgta taatccgcaa tcaagcgatg agcatccgga
    23581 atatttagca cttgtttgca aaactcatat aagttttcta ctttttcatt gtctttcgtt
    23641 cgccaactaa ataaaaactt agggtgcaag tctttcggta aggcatttgc aaaataccga
    23701 gtggtttgtt cattggaaac cacaaacaac tgtaaggtcg aaaagatatt gttccgaaac
    23761 ataccttctt ctgcatattt tttgatttgg ttaaatgcct gataaacacc atcttttgca
    23821 gttacttgtt tcagctcgat ttggacaatc ggtaatccat tgattaataa ggtgacgtca
    23881 aaacgtcgat cccgtgcttc tatgttactt ccacgttttg caatttgatg gaccacttca
    23941 taagtagaga ttcctccacc aatgtcttga ttggaataaa ggataagtga gacagaaccc
    24001 aattgcgaat cttcccgttc aatcgtgatc cgagccatcc cattttcacc ttttaaccat
    24061 ttcgcagcat caaatggggt tttcgttcgc agcagtaatt ccgttttgat cgtttcaaat
    24121 tctttatcgg tcaaaggaaa atcattcagt tctgcttgat tatttgagat aattttttgg
    24181 cgtaaattga cccaaaggtc ttcttctgat tttagatccg gacgatacgt ccactgatta
    24241 tgcccttctc caagtacacg aatcaactgt tcttcgactt ctgcttcctc acggtgacta
    24301 attttcattc ttcttccctc ctagacaaac atcttttgta agaagctttt tttcgtttct
    24361 tttagtaaat ctaacttacg ttgatgaaga gcgatcgtat catctagctg tttgaagaaa
    24421 gtaccgattt tttgttgttc atcctttaca ggaaccttaa cactgatatt ctcaatatca
    24481 gaactgttat aagaaggcaa tgcccctact tgggagtatc ttggcaaatt tatagtttca
    24541 aacaatattt taccaaaata gatatcccaa gtattattaa ataaataagc catggtattg
    24601 ttatctatta agaaagggct tgtgaccaaa cgttttctat taagcattat tgctgcgcca
    24661 actttagcaa atataatcgc tggcacatct tttattggct tccatttttt acgctcaatt
    24721 tgctcattag atacataatg atttgcattt ctcatttcat actcattacc aatgttattc
    24781 atgtcggaaa ctttaaaaaa tggagtacct tcagaaccac cttgctcggc atcaggaaat
    24841 ccaattcccg attgagtttt acctaattct cccaacttac gctcttccca atcttccgta
    24901 aaaccaggaa accggatttc tggcactttt gctccgtttt cagggaacat tttttgtaag
    24961 aatccttttt tcgtttcctt cagcaaatct aacttacgct gatgaagagc gatcgtgtcg
    25021 tcaagttgtt tgaagaaggt gccgattttt tgttgttctt cctcactagg aaacattgct
    25081 actatatttt taaacttttt tagagacaga acatgttgac ctgttccctc tgcttgaata
    25141 gataattgtc gttggattcc attgttaata cgtcttaaga agaagtcgga atcagcagat
    25201 tttaaagtaa atacaggata aaaatagctt ataatccctc tatcaataat gtcattacgg
    25261 ttaaacacaa acacatcatt atcacttcga ctacggaacg tgaaatatcc tctaggtagt
    25321 acaaagtaac caatattatt ttctgtagtt acttgccgat ttgcaaaata gtcttcttgc
    25381 aaaactatcc ctttttgttg agatgaagtt agcataggaa attgattttg tacacttgtc
    25441 ttttcaacat aatctacaac gaagtccccc aacttcctct gttcccaatc atctgtaaat
    25501 cctgggaaac ggatttctgg ttgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
    25561 ttgtaaccac gcttcatttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttt
    25621 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
    25681 atcaattggt tcttcttctt cgaaagtatc tacatagcgt gggatattta agttatagtc
    25741 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
    25801 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat agtttatttt gatttttccc
    25861 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
    25921 caacacaatc accgtcgttg gaatcgatgt cccaaagaac aagttcgcag gcataccgat
    25981 cacagcataa atactgccat cttctagtaa tttttgacgg atgacccctt ctgcagcgcc
    26041 acggaataaa actccgtgtg gtaagacgat agccatggtc cctgtttcct tcaaatgata
    26101 gaaaccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt ttccatagcg
    26161 attaaaccga gaatcatcta agaaggtcgt atctgctgac caattagccg aataaggtgg
    26221 gttcatcact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
    26281 atccccattg cggagattca tttcttcggc gtcgacacca tgtaaaatca agttcatttt
    26341 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctgggtg
    26401 tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa cccatcgttg gatcaaaaac
    26461 actaaagaat cgtcgttctt tttgatccaa cgtcacgatt tgtgccatca tatcagaaac
    26521 catatgaggc gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
    26581 caaaaattca tacgcatccc cgatcacgtc cccatcgtga cctaaaactt ccacgtcatt
    26641 caatttctta atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
    26701 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
    26761 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
    26821 taagacgttg aaaagatact ctggtgcaat tgcatatccc aaaatatcaa cgattgtggc
    26881 aatcagatct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
    26941 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
    27001 tcggtcggat aaatatttat agaaaatcag ccccaataaa taatttttat attctgacgc
    27061 atccatttta ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
    27121 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
    27181 ccccctttta taaaaagcaa aaaaaaaaag aaagaaactt attcttgcta ataaactacg
    27241 cgaaaggagt ctaacgttgt ttgatataac ttagactgat cgttaactta ttttttatat
    27301 ttctacatag ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt
    27361 ttcaagctct atactttttg atgttggtgg atgacaccag catctttttt attttccaaa
    27421 acatatagaa aagacacctt gaaatccttt agaattaatt tggcgaacca acccaaaagg
    27481 aggaatcaag atgtcttaca ctcaccttat caaagaaaca ttagatattc tagacttatc
    27541 agttactttt aacgaaaatt gtctcacaaa agaaaaatac aaagggcaaa tctgtcatat
    27601 ttatcgtgga aacttgattt atacagccca agaatgcatc cactgtaaac accagattgc
    27661 ttcagatatt gtgcgttggg gtacaacaac cgtccggttg ttaatgaacg atgtttctga
    27721 atgccgtacc tacctcgaat tgaaaaagca acgcttcaaa tgcaaagctt gtcagcggac
    27781 ctttgtcgca gatacttctg ttgcgaaaaa acactgtttc atcagtgaga aagtccgctg
    27841 gtctgtcgtg actcgtctga aaaaaaatac ctcaatgaca gagatcgccg ctcagaaaaa
    27901 tctatcggtc tcttctgttt accgtattat gaagcgattt tatcgtccgt tgaatccttt
    27961 tcggacaccg ttacctaagg tactttgttt tgatgaattc aaatcagttc ggggtgtttc
    28021 aggtgctatg agtttcatca tgatggatgg acaaacacag cgcttgttgg acattgtcga
    28081 gaaccgacaa ctgccttttt taaaacgtta ttttagtcat ttttcacgag aaattcgtga
    28141 agcggtcgaa tggatcgtga ttgatatgta cgctccttat gtatcgctcg ttaaaaagct
    28201 gtttccaaaa gcacagctga tcattgaccg gtttcacatc gtccaacata ttggtcgtac
    28261 ctttcgcaat catcgaatca aagaaacgaa ccaactatta aaaagcaaag aacaaaaaca
    28321 ctatcaatta ggtaagcaat taaaaagata ctggaaactt cttcaaaaag atgaacgcaa
    28381 gcttgattat actcgacgcc tttggcgacc tggcttcaaa gctcatttaa cagagaccga
    28441 tattgtggat cgcttactga aagggagccc tgctttgcgt gtaggctatc agttatatca
    28501 agactttctt tacgctgtaa aggaacgaga ctacgtatcg tttgaagaac tacttaccaa
    28561 taatatcatg cttcccgaag gctatcagac gaccttgaga acgttccaga aatttctgcc
    28621 acaaatcaaa aatgctttac aacaaagtta ttcaaatggc ccacttgaat gtctgaacaa
    28681 ccatatcaaa gtgttaaaac gaaatgcata tggctttcgt agcttttata atttcaaatt
    28741 gcgcatcatg attcgtcatg gcaacgcatt gattttcaac taaaaaagat caagaggaac
    28801 tgatccactt gatctccaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
    28861 gccttttttc ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa
    28921 attttttcac taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat
    28981 gaaactatct ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa
    29041 aaaatacccc cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca
    29101 acggggtagg gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa
    29161 tacttcgttg taatcagtta cctgtccgtt cttaaatacc acagaataat ctttttaccc
    29221 attttcaagt aaccgaagtg gaatgtcttt accaacttgc tctgtcactg cattactgat
    29281 acctgttaga ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc
    29341 agtatttatc gtgattcctt tatcttcatt tacaagcttc caagtaggtg gtatagctgt
    29401 gtatgtttcg atgaatgaat caattctttc atctatcgtc ttcccagtat ctacttctgt
    29461 ctcattactc ttagatgaat cgattctttc atccatcgtc tgctccgtaa ctgcttctgt
    29521 ctcattattc tcaccggaaa ctctcttcac acccaaactt atatattgtc catctttagt
    29581 gtgcatagtt aaaattccca cgcttttact aataaattca ctataaccag ttctttcaat
    29641 agagacttca atgtatttca cactatcata ctcaaaagaa aaatagacat gtaaaagcat
    29701 tttgttgttt acatctacat aataatcctt tgactctact tgagaccttc ctatacggaa
    29761 tacagcttta ctgtctgata tctctatacc agcatcctta ttattaatct gccaatctcc
    29821 aagtagctct tctggcaagc tagtagtttc ttgtgaacac tttaccactt cactggtaga
    29881 ggtcgaaaca ttcacctccg cactgttaga agaattttcc aaggaatcag gagccttagc
    29941 acaaccaaca agtctttata tattgtgcag ccgatatttt cttcattcag tacacccttg
    30001 aaatatgcag tgttagataa agcagttcgt cctgatttgc tgaggcaccc atctcttctt
    30061 gaacgtactc cttgattttc aaggccgccc gatactcttt cggaaaacgc tgcttcactg
    30121 aagccagcaa aaaatcatcc ttctccggca actcttcctt cttaatcatt cgggcaataa
    30181 aaaactgtaa atgcgtcaca aatcttaagt aaagcatcga atctttatca accttttttt
    30241 cgggaaacag atatcctagc atagccaaga tatcctttag tgctcttacc gagagcatgg
    30301 tttcttccat atttgcttgt tcactttggg catttacaat gtgaaacgca atgtttccag
    30361 cctcctcttc tggcaactga acattcattt ttttgttgat caattggata gcatagcagc
    30421 cgatctcaaa ttcttttggg taatactttt gaatttcaat cagcaggcga ttttgaaaaa
    30481 caatgccttt ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag gtaaaaaata
    30541 gacttttatt cagcttaaag tcaaacttgg cgttggcata atcgataatt cgcttgacgc
    30601 aaataattag atcatccgga gcagcctcaa acagtttaat gtaatccttt tttagagcat
    30661 cagaaagtga atctaattta aatacttttt ggattttgtc agccggaacc aaatcgcctt
    30721 tctttagttg aaatccaatc cctttcccca tcaaaattac ttctttgcct tgcttatcta
    30781 agcaaagaat taaactgttg tttaacgttt taattactct cattagattc accgttctct
    30841 ctaagctaag ccatttcttg cgattacttt ttgataccaa aagaagcttt ctttttttat
    30901 tcgttttaat tctttaagat tctcatcggt tcggtcaaca tatataaaac cataacgctt
    30961 attgaagcct tgatgggtac taacaacatc aatcgctgac caaggtaaat aaccaaaaac
    31021 atctacacca tctgttaaag ctgcttgaat agccagaata tgtttggcta gataatcaat
    31081 tcgatcctgg tcattaattc gtctatcttc ctcaactatg tcgggtgccc catagccatt
    31141 ttcagtaatt aaaataggta aatcatagcg atcgtacaat ttcctcaatg ttaatcgtag
    31201 tccagtaggg tcaactaccc agccatatgg cgttttatct acataaggat tttcagctgc
    31261 ccggtagaca ccttcttctc ccaacatgat ctgttgatcc cccgctctag cggaaacatc
    31321 tgatccgtca tttttgctgg cagcgatggt ggcagttgaa taataattaa tcgcaataaa
    31381 atcgggttta ccggaggata aaattgcctg atcctctgcc tcaattttag gatataatcc
    31441 acgatcattc aaatagtttt caaacaaata attgtatttt ccccgaactg ctacatctaa
    31501 gaaagaccag cctctcaatg tttcccaatt atgggctgca attgcatctt ctggactgga
    31561 ggtagcttga tacatagccg ttaaattgat tgctggaccg atttttcctt taagattcaa
    31621 ctgatgatac aagtgaatta ctctagcttg agctaataaa acatggtgat taatttgaaa
    31681 gagttctttt tttgaaggca gctctccact tggtggcaaa ccaatcgctc caggatgaag
    31741 gatcatcgtg ttttgctcat taattgtcag ccaatagttc accttgtcac catactcagt
    31801 aagcaatacc tgtacatagt ccacaaaata atcgatagtt gctcgattta accaaccgcc
    31861 ctgctcctct agaaaataag gtaaatcgaa atggtacatg gtgacaatcg gttcgattcc
    31921 ctgattcagg cattcatcaa ttacttgatg ataaaaagaa attccctttt gattggtttt
    31981 attattttca atatccataa ttcgcgtcca agcgatggaa aagcgaaaag cttttaagcc
    32041 taattcttta aacagctgta tatcttcttt atagtgatgg taaaaatccg aggcaacttc
    32101 aaaattagct accccttctg tatgctgata cttgtcaata atagacggtt gtttcccatc
    32161 ctcctgatag gcaccctctg cctggtaggc actaatggag gcaccccata aaaacttctc
    32221 tggaaatgct gaaagctgtt tgtattgcac aaaaatcctc tccttaacta atttgcatca
    32281 atggttcgtg ttcctttaag cttccgcttg cttcactaat tcctgcgtaa ttttcactgt
    32341 tggtcactac cacaatcact gtatcatcgt agccagcgtt tctaatggca gcgaaatcgg
    32401 cgttgattaa taattgtcct gctgacacct gatctcctgc ttttacaaat acttcaaagg
    32461 gttccccttt taaattcacg gtgtcgatcc ccacatggat caataattca ataccttctt
    32521 tagaacgtag accaatcgca tgtttagatt ctaaaatagt aatgacttct ccctcaacag
    32581 gtgaataaat gctgttttct tctggatgaa tggctacgcc ctttcccaat agtcccttag
    32641 aaaagacagc atcgttcact tcagaaatag gacacacggt tccttttacc ggattttcaa
    32701 caatgactgc ttcggctatt gccgtttgct ctgattcatc agaagtatcg tcaaatccaa
    32761 ttagataagc aatgataaag gttgcaacaa aggcgattac acacgtaata attgcgtgaa
    32821 caatattcat tggattttct ccaataaagg ctggtaaggc tgctaagcca ggggaaacaa
    32881 aggcatatct gactaaaccg ctcaagccag catacaaacc agcaattgca ccaccaatca
    32941 tggaagcaac taatggtttt ttcagcttca aggttactcc atagagtgaa ggctcagtaa
    33001 ttcccattaa agcagtgatc ccagtggaca cagctagctg cttcaactct ttgtttttag
    33061 ttttcgcggc tacagcaaag gtagcacctg attgagcaat attagaagcc agcattcctg
    33121 gcccattgat catttcagaa cccgtactgc tcaattgcat agtagcgatt ggcgtcattg
    33181 cccaagctgt acccgtaacc accaaaaatg gctgcaaacc acccatcaaa aatggaatta
    33241 accaagctgc tttgccgtta atccattcag cccccattgc tataagatcg ttcagcaacg
    33301 ttccaaacgg tccaatgaca accaacgcta aaggccccat aattaaaaat accagcatcg
    33361 gcttagtaaa gaatttaata aacgaaggcg aaattcgatc cgcaaagggc tctacatagg
    33421 acatgatcca aacagtaaag ataatcggta aaactgatcc cgcataatct gcagctaata
    33481 ccggaattcc tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa atatttggat
    33541 gaagcaaaat gccagcaaca gtaatcgcca aaattgggtt gacatcaaac tttatggctg
    33601 ccccataggc taatagtact ggcataaaat aaaaggctgc atcagcaata gtattcaaaa
    33661 tgatgtaagt ttgactactg tctgatagta caccagtcaa agtcaagacg gccaaaatgg
    33721 cttttaccat cccggcacct gttatagctg gaatcatcgg tgtaaaggtg gtagaaatga
    33781 cactaatcaa tcttgaaata atactttctt gattgtctga agaggggccc tttttaattg
    33841 aagttcccac ttccttttga atttccttga aggctgtttg gacttcattg ccaatcacca
    33901 cttggtattg tccaccgcta tccaccacac taattacacc aagtgttttt tctaaggctt
    33961 tcgtatcggc tttcctttta tctttgagtt caaaacgcag tctggtggca caatgggtta
    34021 agctgaccac attttctttt cccccaacct gttgcaatac atttcttcct acttctctgt
    34081 aatccattat tctctctcct taaagcagta aaaaagacca agtcaaacca cgccccaatc
    34141 aacattgaag tgttagtttg tcttggtctt gcctgcataa tcagtaacat gccaataacg
    34201 ttattttgct gtaatccaat aatagcacat tatgatagcg caatcaacag ggaatttaat
    34261 tttttatctt ttcgtttgac cgtatagggt aatataacag ttagatcaca tcaactaggg
    34321 tatatctgcg tatacttact gctaaaataa gtatatctaa tattaactat accagtaaaa
    34381 ccattcctac ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa aatgcatata
    34441 acggatcaca caacttcaac aattctgctc ttgggaaaaa ttgatgaatt tggtgagtaa
    34501 aggatggaat aaacagcatt ccaacaatat aggcaacaat agtgatactt gtttttttgg
    34561 taaaaatcag taagcaatag ccaatttcaa tcattccgag atataaaggt atcgatagaa
    34621 tgatttgggc tagtattctt aaaaattaac tagcaaatgt ttaaactgat ttatttctaa
    34681 tctacatgta gtaagtattt caattccacc aattatcaat aaaaatacca aacatatcca
    34741 taataatttt ttctttttca tttcttttat attgttataa cagtgaataa cataaaaatt
    34801 cccgacaatc attgtaatat ttttttgtca cctgtattag tatttaattt ataggaaact
    34861 ataaaaactc cctagtgaat ttacaccttt gggagttcct gtttggacgg tatgcataat
    34921 acatttaaat caatttaaca ttaacacctc agcatatggt atctccacat atatacggtc
    34981 tttctttgga tagtactcaa tatttatgtt gatagcactt ccattgtcta atatagcttt
    35041 agcccggtat gtgttatctc cattttcatg agtaatcgtc acatcatcac atgatcgatt
    35101 taagttgtac tgttcttgta aaatctgtgt taccaatggt tttgatgact tctctaatcc
    35161 agatgtacct gtcatttttg aaaaaataat acctataata gccaagacaa caatacctat
    35221 aatgacaaac ttcttgttca tatttacttt attcaacttt ttatctccta tctccaaaat
    35281 aatttgttct tatccctctt atcagtacat tgacatccct aaaagtacac caaagattgc
    35341 tccagctacc cctgctatca tcaaaatcgt acccattttc tcatcttttt ctcgatataa
    35401 gtagcccata ataacagcaa cagcaccaaa tacaacagga ataaatgcta acgatactac
    35461 catagaaatc caaccaataa tcaaatacag cttccctgtc tcttgtccat gtacgatttg
    35521 ttccttcatt tcattaattg tctgctcagt acttttcttt actgtgtctg ttccggttgt
    35581 ctcatctgtt tcttgtagtg ctccacaaaa ctggcaatac ttactttcat ctgttatttc
    35641 tttgccacat tttctacaat acatgtcttc tcctcctaaa atcattcaat tgcttattag
    35701 ttaactgttt actttattaa atacatcaga aaacagctca ttcattggta cacttaatat
    35761 ctcagaaagc atcttcattt gatagggttt aggaatcgtt ctccctgttt gccacgaact
    35821 tacacattgc tgtgtaacat tcattttttt gctaactccg tttgagaaag tcctttttct
    35881 tttctgtatc ggctgagatt acttactttt ttcatgattc tcccctcctg ttttattata
    35941 tacaacaata tggtgtattt caactcataa aatataaaaa acttcaaata ccattacaaa
    36001 tacaacaact tgttgtacta tactgagatg ggggtgtatt aatgaaacag aaacaatatg
    36061 caaaggtcat tagagaaaag cgaaaagctc tcgggtggac acaaaaagaa ttagctgaaa
    36121 aaatcttctc tacacaacag gcagtagcaa gatgggaaaa ttctgtaaca gagcctaacc
    36181 tcgatagctt aacagctctt tctagagccc taggaacccc tgtaagccat tttctagaca
    36241 atgttgtcgt agattatgag gaagagtttt tggctctcta tcgttctctt agcactgaag
    36301 atgcagttcg aacaattgac tatatgaagc tattaaaaag gcaagaaaat gaacgcaatc
    36361 agctattgaa agattgaaaa ataaataacc caccgaactt taacggtggg tgtattgaga
    36421 tgaaataaga ttaagagcta tggataacct gtctagttta atacaattgg gtcttttctc
    36481 ttccttactc tccgtccatc atctcttgtt acacttgtaa attgatacag aggaactatc
    36541 ccagctcctg ataatatcaa ttcttttatt gtttcatcta tccctgcccc tttcgctaaa
    36601 tatattaaaa aactactact tgctttcaca ttgtatagtt tcttaatatc cttagaagct
    36661 cgttcaagag tgaatccttg tttaacaaag tagcctctgt tttttattaa agcatttttc
    36721 acgctgttat ccccaacatt acgtaggcgt tcacaaacag tgttaataag ttctttctct
    36781 gtcattagat atgtcccttg ccacgataag ccctgtaaaa cttcatgtgc ttcatctaaa
    36841 atcaattgtt tttccttgct gagttctgat cgggaatttt gaggaaatac tcttttagcc
    36901 tctttttcgt taaaacatat cttaacccat tcataagaaa aatattgata tgtaaaatga
    36961 attttttcca gaagtggaag gatatcttca ttcaattttt gaatcacaga agttgcatta
    37021 aaaacgtcta atcgatagac attgcttctc ttatgattaa agtttcctaa cgctttctct
    37081 tcctttaacc tatttaggta ttcgagcatc ttcaaaggaa catcttcatc atctaatttt
    37141 tttatcaatc ctataaaagt cataagtgat agtaacttat tacatttatc tatgtcataa
    37201 gttttaccat agtaagaact cattttttta gctaattgtt cccccgacaa aatacaagtc
    37261 acactttcct tactatcatc aaaaaaatta acatcagaaa gcaagacatc atagacacgt
    37321 cgcatttcct ctagataatt ccttattgtc ctgaaaagtt tgtctttcat tgattcaaga
    37381 gtgtcaaaaa gtacttttga ttcctctttc aattttcgaa aatgtgtagg atacaagtct
    37441 aggttctcta atagccaatt aaatgtttct acccgactgt ctttctttaa aattagcatc
    37501 acaactccaa taaaattttc actgatatca caattactgg aatgacaata aaaaatggaa
    37561 tgcccagttt tactggtaaa aatactagca gagggattat gatcgttgtg aaacagacat
    37621 cttaagtttt tacttgactt aagtaattta ttcatatcaa tggttaacaa tctttcataa
    37681 atctcattaa aagacatgtc ggaggaatca aacaggcagt ctttaaacca ctgtttcatt
    37741 tcttctttgt cgtcattttt tatcaactta acaaaatcgg tttcattgac tagttcatac
    37801 tctctccctg aatcactttg acaattttta gaacatgtca tggtaaaatc aatcggaagc
    37861 tcagtaatat ctaacatgtt gtcttcatta attattatca ctgcatctga cttatatcct
    37921 ccaaaaaaca ttctattcga agcatttaca ttgtcatcac acccggggat gttctttttc
    37981 aagtattcgt aaacagcaac cattttacca tagtcagtaa ttgcttcttt taatagaaag
    38041 acaatcttgt atttttctag attttcagag taccgaatac ttttttgtat cgcaaacgca
    38101 gaattatgaa ctaaattgat agataaaacc ttttctatgc ttaagtatct actatcatct
    38161 ttactcaaat ctactacctt ccccttctca tctttaacag tgttatcgac atccagaata
    38221 attactctgg tttccacaaa actctccttg tcattgcctt tattctttgt cttgcttgga
    38281 ataaatgatt gtgatgaatt tagtatctct acaaactcat ctcttgtaac ttctctatag
    38341 ttatctaaac acctatctct tacatcagtt ttttcttttt ttgtaactgg cttctctaat
    38401 aatttttctt gatctacata aattggaata ttaatcattg atgtttccat ttgtgtttcc
    38461 tccttataaa attctgttaa agactaaaac atacagataa taaaaacaaa ctctttatta
    38521 ctatatgttt tagtcttatt tgtttactca acctttatac cgttccatga aactatcaat
    38581 atcagatttt ttaaatctaa tgaccgtatc aatttttata acaggtaacc cgtattgaga
    38641 aacatatttt ctaatcgttc ctactgatac acctagatat tcacttgcct gctgatagtc
    38701 catatactct agagtgactc cagaaataat gtcttctagc ataggcttta gcactgtaat
    38761 tatctctttc acttgttgtt caacttgtat agaaacaatg ctatcaatga tttgtagaga
    38821 catatttccc cctcctttcg tgattgcttg aacaaatttt ctaagaatta tcttcgtatt
    38881 ttttgctcaa aataaaagta ttttgttatt aaccgattaa tcaaacaaaa aatgattacc
    38941 agttcatcga catacacata atacctcgat aaaaaagggg tgtcaacagc tttgtaaaat
    39001 ttatttacaa cacccctttt ttgaggtata ctaattttag gaggtgtggt caatatgata
    39061 aaacgtccac caataaatta tttagaacga aaaaagatac tcggaacaaa aataaaagct
    39121 ataagaaaga gtaaaaaatt aacacaacca gcatttggtc ttatgattaa caacggtcaa
    39181 ttaattgaca aaaaaactat ctacgaatgg gagaagggaa cctatttacc aataccagag
    39241 cgattatccc gaattgctga tttgggaaat atgagtatag aggagttagt ttgtagcaat
    39301 gtagaagaat acattttggg aattattctt tatagagata gcattgtact agatggtata
    39361 acatttccag ataaaaacct gttccagcat cttcgtcaac agttcccacc agtacactct
    39421 aatttagata catggctgga tagatattcc aaattagaac cagagatgca agaatttatt
    39481 gctaacaaga cttgtaataa agttaaaaat gaaaaaatta gtcttttcaa tatcttaaaa
    39541 atagaagaat tattcatcaa tgctattgta gaagaatttg acaataacat tcttttcctc
    39601 actagcagta tagaagaact actagaaaga atggtagatg aatggctacc tattcaattg
    39661 aaagacatgt cttatcccga agaagctgtg agagaaatta ctgataatat aaacaagcta
    39721 gaacaaacga tttcatcaat tgggaaaaaa tacactaaga aaaaaatgaa gggaggtgat
    39781 accatctagt ggtaagtact ccttgtaaac taagaggaga tgatttaatg gcaaaaatat
    39841 caaagtacaa aaagaaaaac ggtcaagtgg cgtggatgta taaaggacac attgccaccg
    39901 atccacgaac tggtgaaaaa ataaatacta cgagaagagg ttttagtagc aaacaagaag
    39961 ctcaaaaaga ttatgacgag tacagacatc aaatattata cggtgttaaa aagaaagcac
    40021 ctgatatgac atttgaagac ctgtataatg aatggataac tcatcagcgt accagtgtta
    40081 aagcttcaac aatcgcaatc tcagtaagat acgcaaataa tcaaatactt ccagcatttg
    40141 gtaagcttaa actatcgaat atctctgtcc catattgtca aaaagtggta gatgaatggc
    40201 acagtaaata tgaaagttac gattatatga gaaaacagac tgcacaaatt ttacgctacg
    40261 gggttgcaat gcaatatatt gacaataatc ctatggaaaa aacacttctt ccacgaaaaa
    40321 aagagtatga gaaaaatcgt aaattttact caaaagagga gcttaataat ttactggatg
    40381 cttttaaaga ttttggtaac atgaaacaat acgccttctt cagattattg gcatacacag
    40441 gtatgagaaa gagtgaagta ctcgctttac agtggaaaga catagacacg tttaataaag
    40501 aactacatgt caataaaact cttgctgtag atgaatttgg taaagttata attcaatctc
    40561 cgaaaacaag agcttctaga cgagttatct ctttagatac tgagactctt tctatcctca
    40621 acaactggaa attacagcaa aaagaagagt atttaaaact tggatacaat actagctcta
    40681 aagagcaaca tgtttttaca acagttaaaa acactctata cattccaaac actgttaatg
    40741 attggttaag atatattttg aaaaaatata acttgcctcg tattacacct catgggttta
    40801 gacatacaca tgctagttta ttattagaag caggagaatc tgtcaaggta gttcaacaac
    40861 gattagggca tgaaaatagt aaagtgacat tagacatcta tgcacatatt acaaataatg
    40921 cacctaagaa aacaggtcaa gactttgctg atatgatggc tcatcagtaa aagagctggt
    40981 caaaatctgg tcatttctag tatacaggca aaacaaaaaa gctagaaccc ttatatatca
    41041 agggttctag ctcctataac aggtttaccc aacactgcct tccatctcat aactgatcaa
    41101 tcgattgagt tcgactgcat attccattgg aagttctttg gtgaatggtt caacaaagcc
    41161 catgacgatc atttcagttg cttcagattc ggtcaggcct cggctcatca gatagtagag
    41221 ttgttcttct gagatttttg agactttggc ttcgtgttct aaagagactt ggctgttgtg
    41281 gatctcattg aatgggatcg tatcggattt cgaccattcg tccatgatga tcgtgtcaca
    41341 ttcgatatga gagatcgaac ccgcactgtt tttggcaaag gttacttgtc ctcgatagtt
    41401 tacttctccg ccgtcttttg aaattgattt agagacgata gagctagatg tatttggtgc
    41461 attgtggatc atttttgcac ccgtatcttg gatttggttt gctcctgcaa aggcgatgga
    41521 gagcatggtg cctcgtgcac cttttccatc taagtaaaca cttgggtatt tcatcgttgt
    41581 ttttgcacct aggtttccgt cgatccattc aacggttgcg ccttcgtatg cttttgctcg
    41641 tttggttact aagttgtaca cattgtctga ccagttttgg atcgttgtgt aacggcaata
    41701 cgcatctttt cttgtaaaga tttctacgat ggcggcatgc aggctgttac ttgaataagt
    41761 aggtgccgta caaccttcga catagtggat gcttgctcct tcgtcaacaa tgatcagcgt
    41821 acgttcgaat tgccccgtgt tttccgcatt gatccggaag tacgtttgta atggaacatc
    41881 gactttgacg ccttttggca cgtaaataaa ggtcccacct gaccatacgg ctgaattcag
    41941 cgctgctaat ttgttgtcgg ttggcggtac aagttttgaa aagtactctt tgaataattc
    42001 agggtattct ttcaaagcag agtcagtgtc agtaaagacg attcctaact tttcgaattc
    42061 atctttcata ttatggtaca cgacttctga ttcatattgt gcggaagcac cggctagata
    42121 agctcgttca gcttctggta taccaatctt ttcaaatgta tctttgattt tttctggtac
    42181 gtcttcccag tcacgagcgg gtttgtcact tggtttttgg taatatttga tggcatcgaa
    42241 atcgatgtct gaaaggtcgg ggccccatgt ttgcattggc attttgttaa atgtttctag
    42301 agatttcaga cggaattcaa gcatccattc tggttcgcct ttgattcttg acatctcacg
    42361 gatcacttct tctgtcaagc cttctcctgt actgaaaacc ggttcgacat catcgtgaaa
    42421 cccgaattta tattcttcta attctggtac gcccatgttc tcactcctct tccttgtggt
    42481 aatgatggaa agctgcggta ccatcgtttt ccattgcttt tcctagtgct ttccaggcaa
    42541 gtgtcgcaca tttgatccga gcgggaaatt tagctacacc accaagcata gcagcatcgc
    42601 ctaattggtc gggatctttg acttcttttc cttggacaag ctgtagaaag tctacagtta
    42661 aatgttctgc ttcttctatt gattttccga tgacggcgtc tgtcatcatc gaggcacttg
    42721 ccgtactgat ggagcagcca ctgccgtcaa aggcgatatt tttgatgatt ccgtctttta
    42781 catcgacttg caattcgatc acatctccac atgtggggtt gttcaattcg atcttgcttg
    42841 tcgagtcagt caagcttcca cgatgatgcg gatgggaaga atgatccaag atcacttgac
    42901 ggtataaatt gtctaattta gatagtccca tgttggaaaa actcctttgt tgccatgatt
    42961 gcttcaacta aacggtcggc gtcttgcgtt gtgttgtaga aataaaagct cgcgcgagca
    43021 gtcgctgata cttcaaggta cttcaatagc ggttgcgcac aatgatgacc tgctctgacg
    43081 gctacccctt ccatgtctaa agctgtcgcg acatcatggg gatgtaaccc atccaaattg
    43141 aatgcaacga ctcctgtccg aaaattcggg tcttgtggac cataaacagt caatccatca
    43201 attgcttgta attttggcaa cacataagcg gctagttctt cttcatatcg atggatattg
    43261 tccataccga gtttttgcaa ataatcgatt gcatggccta atgcgatagc gccggcgata
    43321 tttggtgtcc ctgcttcgaa tttccaaggt agttctttcc atgtactgtc gtataattcg
    43381 acaaaatcga tcatttcgcc accaaattca acaggttcca tttcttctaa ccattttcgt
    43441 ttaccatata aaacaccaat acctgttggt ccgcacattt tgtgtccgct gaaagcaaag
    43501 aaatcacagt ctagtgcttg gacatcaatg gccatgtgcg gggcagattg agcaccatct
    43561 actacgatga ccccgccgtt ttcatggaca agttcagcta attcttgaac aggattgaag
    43621 acacctaaga cattggatac atgagcaatg gagacgattt tggttttgtc cgtaatcttt
    43681 ttcgctgcat cctccatatc caggaagcct tcttctgtta gttcgatata tttcaagaca
    43741 gcacctgttc gttttgccaa ctgctgccac gggataacat tagaatgatg ttccatgtaa
    43801 gagagcacta tctcgtctcc tgctttgata tagcggtcgc cgaaggttct ggctacccaa
    43861 ttcaagcttg tcgtcgtacc acgagtaaac agcacttctg ctgtttcttt tgcatggatg
    43921 aacgcccgta ctttttctct tgcttgttca taatcatggg tcgcgcgttc tgctaacgta
    43981 tggacaccgc gatgaacatt ggcgttatcg tgttcgtaat agtacgctaa tgcctggagt
    44041 acttgttttg gcttttgggt cgtcgctgca ttatccagat aaactaaagg ttcatcattg
    44101 acgtcttgaa ataagatagg aaaatcattg cgtagctttt tagcatcgat catccattca
    44161 acttcccttc gattacttcg actaattctt tccgaacttg ttctacaggg attgctgtta
    44221 agacagaacc gaggaatcct cgaatcacta agcgctctgc ttcttcttta tgcaatcctc
    44281 tactcatcag atagtacatt tcttctggat caactcgtcc tacactggca gcatgtccag
    44341 cagttacttc gttttcatcg atcaaaagaa taggattcgc atcaccacgt gctttatcag
    44401 aaagcatcaa gacacgactt tcttgttggg catctgcacc ttttgcccct ttcaagatgt
    44461 ggccgatacc attaaaggtc aatgttcctt tttctcggat cactccgtgt tgcaaaatat
    44521 gcccgatcgt atgaggggct ttattggtta ctcgtgtatc gattccttgg atttgtctgc
    44581 ccgagctgat tgccacgatt ttgacttctg cgtgtgcacc ttctcctgct aaatctgagt
    44641 caaagtcagc gatcacatga ccgtcattca tgacaccaat tgcccagtcg acagaagcat
    44701 ctcgtaaaat gtgcccacgg cgattcatat aagaagtaat attttctcct aattgatcta
    44761 cggctgaata tttgattttt gaaccagctt tagcgatgac ttccacgatg atattacctg
    44821 atgattttgc tacttgttct ttggttgtct ggaaacgttc cagataagaa aattcactat
    44881 gttcatctgc cacgatcaac acatgcttga agaagtgctc atcggatgcg ccatcttgaa
    44941 tgaataagga ctcgagtggt tcatcgatca cgacattttt aggcacatac aggaaaatac
    45001 cgctgttcat aaaagctaca tggagtgcag tcaattggtc ttcgttcatg tttactgctt
    45061 ttttcatata atattcttgg actagttctg gatattcgat cattgcagtg aacaagtcgg
    45121 taaaaatgac tcctttttct gctaattcca caggcagttg ttcaaaaatc gtccatgaac
    45181 cttgttggac aagcacagga ttgtctttca ttgcatcaaa tgccggaatc gtgcctgttt
    45241 cactgatggt ttgtgtttct ttcacatcaa ataacgacca gcgatggaat ttcacccgat
    45301 cgatatgcgg caatggcagt tcatctgctt tcgctagtgc gtcttgacgg aaggttctca
    45361 tccaatcagg ttcgccttta cttaaagaaa aatcatttac cgcgtcaaga taatcgagcc
    45421 agtttttttc tttcattctt gctccccctt acgcttcttc ttctttatag tcgattccta
    45481 attctttact gataccagcg tagccttctg cttccagacg tttagctagg tcagcattcc
    45541 ctgtcatgac tactcggcct tccatcatga tgtgaacgac atcaggtgtg atatagttca
    45601 ataatcgttg atagtgggtg atgatcaatg caccgaaatt gtctccgcgc atttcattta
    45661 ctcctttagc aacgactttc aaagcatcga tatccagacc tgaatcgatt tcgtctaaaa
    45721 ttgcaaatgt aggttctaac atcaacaatt gcaggatctc attccgtttc ttttctccgc
    45781 cagaaaaacc ttcgttcaga taacgttcag ccatttcttc tggcatattt agtaaagcca
    45841 ttttttcatc taactttttc aaaaatgcca taacagaaat tttgtcgttt tcatcacgtt
    45901 tcgcattgat cgctgcacgc atgaattccg cattcgtgat acctggtatc tcacttggat
    45961 attgcatagc taaaaataag ccaagtcgcg cacgttcatc tacttccaag tctaaaacat
    46021 ttttcccgtc aaacaaaatt tcgccttctg ttacttcata gtttggattg cccatgatag
    46081 cggcagataa ggtagatttt cctgtgccgt taggtcccat gatggcatgg atttctccgg
    46141 ttttcattgt taggtttacg cctttaagga tttctttttc ctcgatcgac acatgtagat
    46201 tttttatttc taagacagac atgcattata cccccaaatt ctctttcgtt ctcactcatt
    46261 ttagactaat tgagaatgaa gcgcaatgac cttccttact aaaatcaaat atcttttatt
    46321 tattattaaa caattttgat ttctaaatta gaataggtat aaaaaagatg agaaataagc
    46381 cattacagac ttatttctca tcttttttgt tccatacctt attttgcatc agcctttact
    46441 ggtttaacag agccgcccca tttgtctgtg atccattctt ggacttcttt gcggtgaagc
    46501 acttcaatca gttttttgat tttgtcactg tcttcatcac ctttacgagt cgcaataata
    46561 ttcacgtaag gagagttgtc cttttcaagc aatacggcat cttctaatgg attcaaccct
    46621 gcgccgtaag cgaagtttgc gttgattgca actaggtcac cttcatcatt ttggtacgta
    46681 gaagctaaaa gtgatgggtc gatcgtatgg ttgaacttca agttcttagg gttttcttca
    46741 atatcatcga atgttgctgt ttccagatct actccatctt tgactttgac taagccagca
    46801 tcttgtagaa ttgtgatgat gcgtccccag tctgattcag aatttgaagt aatgattgtt
    46861 gcaccatctt ttaattcgga aacatctttg acttttttag agtacaagcc cattggttcg
    46921 atgtggacag caccggcatt gacaaagtca tatccttttt ctttgatttc tttgttcaaa
    46981 taaggaatat gctggaagta attggcatca atgtcgcctt cttccaaaga ttggttcggt
    47041 aatacatagt catcgaattt gacgatttca agatcgattc cttcatcttt caataatggt
    47101 ttcacatgtt ccaaaatctc tgcgtgcggt gtcggtgaag cgccgacttt caatgtcgtg
    47161 tcgtctgctt tatttgctga atctttgctg tcgctgttgt taccgcatgc tgctaatcct
    47221 acagtcagca aaagcgctgc tgcaaatcca aaaattttct ttttcatatc ctacatcccc
    47281 ctatatccta ttatcttttg tctgttttct tagttaagaa atcaccgatt ccttgaacga
    47341 taaatacgat tagtaaaata attaaagtcg caacgaatgt cactgttaga ttaccacgct
    47401 ggtagccttc ctgccaagca agacctccaa gtccacctgc gccgatcgcg ccggccatcg
    47461 cagtaaatcc gatcatcgag atcgttgtaa ctgttaatcc agaaatcaat gcgggaatac
    47521 tttcaggtat caagaccttc cagatgattt cccagtaact tgctcccatt gcatctgctg
    47581 cttccagtac accagggctg acttcacgaa aagcaatctc tactagacgg gcataaaatg
    47641 gtgctgcgga taaaacaaga gctggaatgg ctgcttgcgc acctagcata gtgcctacga
    47701 ttgctttagt aaatggaatg atcaaaacca tcaggatcat aaatggcgtt gaccggaaaa
    47761 tattactgat gatcgatacg attccataaa tgattcgagc atacggtttg tttttgcgtc
    47821 caatagagta aaggatcaat cccaaaacta atccgatgat cgtcactaag accattgaga
    47881 ttaatgtcat atatagtgta tcaaggatag cctgattcat cgtttccggg ttgatttgtt
    47941 taaaatctag atatgtttct gaaaagcttt tatccacgat ggatcacctc aattcctact
    48001 ccttgttctt tgatgaacac ttctgcttga tctaattctt tggtttcacc agtcatcaag
    48061 acagtcaatg aaccaaagga accttctttc acttgtttga tctttccata cacaacgttg
    48121 acatcgatgt tatagcgtcg aatcgcttgg gaaatgactg gctcgttggc atttctttcg
    48181 ctaaaggtca aagttgccaa acggccgctt ggattttctt tgatgagttc agctaatagt
    48241 tctgtcgaat cagcttctgg ttcgatgtct tgttgtacaa agcgtttggt tacttctttt
    48301 ttcggttgtc tgaagacagt caatacgtct ccttcttcta cgactttccc caactccatc
    48361 acagcgactt tgttgcagat tttgcggatc acattcattt catgtgtgat caagacgatg
    48421 gtcaaattca atttcttatt gatcgctaac aataagtcta atacttcttc tgttgtttgc
    48481 gggtccaatg cgctagtagc ctcatcacaa agcaggatct caggatcatt tgccaatgct
    48541 cgtgcgatac cgacgcgttg tttctgtccg ccggaaagtt gtgaaggata tgcttgtccg
    48601 cgtccttcta acccgactaa acgcaacagt tcttctgccc gttctttacg tttgctcttt
    48661 ggtacgccag ctaattccag cggcagctgg atattttcaa gtacagttct tgaccataac
    48721 aggttgaaat gctggaagat cataccgatt ttctttcgga atgtacgtaa ttctttgttt
    48781 tttaattttg taatatcttg atcgtttacg atcacttcgc cgctcgtggg aagctcaagt
    48841 ccattcagga gacggactaa tgtacttttt cctgcgccag aatagccgac gattccataa
    48901 atatcccctt tttcaacgtt tagtgaaacg tcattgacgg cttggatgtt tccgtttttc
    48961 ccattgaatg tctttttgac attcgttagt tggattaatg acatgccatt cacatctttc
    49021 tcttatgatt tttcaaacaa aaaagccttc gccctcttgc cttaagcaaa aggacgaaag
    49081 cttgaacttc cgtgttacca ccttgattta ccgttacttc gcaataacag cctcttccag
    49141 tacttggact tagcttcttt ccgtatactg tcgcgctgta acgggcactc ccgtagtaag
    49201 tttgcttacg ctcgcttact ctgctctaag accatcttcc atctcatttt cgttacccat
    49261 tttcagcaaa cagggctctc ttcaaacgtc cgtgacatgt actcttcttt tcaaagcttt
    49321 ctcgtttcat ataggattgc ttatgattat agcaactgga agaatcagtg tcaacttttt
    49381 tcaagtgatt ttctgatttt ataattcgtc aaattctttt ggatcaacat ctttgaaact
    49441 taagatccac gcattcattt catctgaatc attcaagata ttgatatctt caccgatttt
    49501 atcattaaca gaagaaactg tacccgttag cgggctggca aattcattga ccgctttttc
    49561 cgcttcaact tcgatcagag gttcatcttt ttttagcgat tgtcctgctt ttggcagatt
    49621 ggcaaacgtg atgtctccaa gttcttcttg cgcttcattt gtcaaaccaa cacagtattc
    49681 ttttccatta aataagatcc aaaggttgtc ctttttcttc aaatgcttgc ctgccatttt
    49741 atatcagttc tccttcataa attttttcat tgaatccaat tgcaacaagc tggtcctctc
    49801 gtaagatcag tggtcgcttg atcagcatac catctgtcga taagacttgg ctcgcttttg
    49861 aagccgtaaa ctcgcctact tgatctttca gtccaagttc ccgatatttc atcccgcttg
    49921 tgttgaacag gcgacggatt ggtacttgac tcgtttctag ccaagtctca atcgttgagg
    49981 ccttcggtac ttctttgatc atatcaacgg tttgataact aacgccatgc tggtctaacc
    50041 acgctttggc ttttttgcat gttgaacatt tcgggtacca gtaaaaggtg tacaattttc
    50101 ttacctcctt gaagatcttg caggtgcttc attttggtat ctcatcgata atatcaaccc
    50161 gataccgatc atgttcccta aaatcgagga tcctccctga ctaatgaaag gcaatgggat
    50221 ccctgttaaa ggaagtaatc cgatatttgc tccaatattc tcaaagacat ggaacagcag
    50281 catcatgatc aaaccgcttg cgatataggc gtaaaattca ttattcgtat cgaaacacac
    50341 ccgaatcata cgatagatta aaataaaata caagaaaatg acgaatgcac caccgataaa
    50401 gccgaagttt tcaccgataa cggtaaaaat catatcggac tcacgaacag gcacatagat
    50461 attgcttacg ttgaatcctt taccaaacaa gccaccggta ccaattgcca aaagtccttg
    50521 ggccggctga taactcattc ctgacgtatc atggaaaggg tccagccatg aatcaatacg
    50581 tgcaaactga taagatttga atccaacttt ataaagcaag tctcttccgc cttcagtcgt
    50641 aaccaagaag atcgttcctg ctccaacgac tactgccaag attactacag gtgcgatgat
    50701 gcgccaagag attccagaca tcaggaaaat cccgccaaag atcgcaagaa agacaagcat
    50761 cgttccaaag tcatcttggg ccatgatcaa tgctaaaacc ggtattgtca cgatcaagag
    50821 cttcccaatc agccaaaagt ccgatttcat cgtccgttct tttacttgcg tattatgttt
    50881 agttacgatc agtgccatca tcaagatgta cgcaatcttc atcaactctg ctggctggaa
    50941 agtaaacgca ccgaatcgaa accagttttt agaccctgtt gaagcagcta aattctgatc
    51001 atagaatttg agcaagagtc ccattaccac taaccccaaa ccataaaggt aaggagtcag
    51061 tttccagatc catttggaat tcaaatgcat gatgacaacg attgctgccg ctccaactaa
    51121 ataccagaca acttgcatcc ctacgccttt caagactgct ggattgttcg gatcatggct
    51181 aagtgctaca tataatgaga gcagaccaat caaacataac agaaagactg gtaaaatcac
    51241 gccataatca atacgattat cgttcgaagc ttttaatttt ttttccatgt ttcatccctt
    51301 ttcttaaaaa ccatactgct gattattata gcggtccagt tagataatgg aaagtctttc
    51361 catagacatt tgggaaaaat taactttccg tttgtgcagt tgcttgagca aatgcagtac
    51421 aaaagcctac tgcttatctt tagtgtatca tagaactttt tttgatacat gcaattatct
    51481 accagctgag attctcctcg ctgtgagaag tttctgtgct ttcattgtat cgaaggatct
    51541 cttcggcaat ctctagcagc tgatcagaaa ccagattggt tcgttggatg aaataaaaat
    51601 atcgatggaa attcaacgga gtcattggca cattttctgg aacagagcgc ttagacaaaa
    51661 tcgagcgacc gattccttcc ttcagcaacg caacaattac atcgttactt ttcacgatca
    51721 tcttcttatc ttggatattc atctcttcaa aataacgctg cgtgtaatga aaaacaccag
    51781 agtttgcttc tcttacaagc cacaatttgc tttttgggtc cccagcgatg actagctggt
    51841 cttccatcaa agagtatctt tgtacgccgg aagtctctaa tggcttttcg ataaaaccaa
    51901 aatcggattc atgtttcgct acttgttcta atgattcatg agaattcgtg atttcgatct
    51961 caaaatgcac tttaggaaac cttttgatca gatgtttcat caactgaggc aaaagataga
    52021 cagcaaaggt gtgggaagca acgatccgac atacttcttc ttcattttgt tcattgagca
    52081 atttttgctg agtttcttcc cattcttcta acaaaaccag tgtccgttca tataataagt
    52141 cggcttgctt agttggaagc atctcttttc ttccgttacg ttgaaaaaga tcgatattca
    52201 attcccgctc caactgtttg atttgattcg agactgctgg ttgagataaa aacagtactt
    52261 ctgctgctct ggaaaaattt cttgtttcat atactgctcg aaaagtcgtt aataacttaa
    52321 acatggcacc acttccataa ctttttatga taattactat aaaaactatt tgatttaaat
    52381 atacaaaaaa ccatctataa tagaaatgta caacaaaaca gttgctgatc caactagtaa
    52441 cttagatgaa gacaatcagc tgtctgcttg ctcgtaatca tacattccta ttcattttac
    52501 aatgtattcg ttcatcttgg tactaaaatc tattaaataa aaaggagaat attatatatg
    52561 aagaactttt tgaaaattat tccgatcccg atctgcgggt tgatccttgg gttagcttcc
    52621 cttggaaatc tgtttaaaga ctatcaccat gttgctttag gcaatctaac tggcggtatc
    52681 tctatgattc ttatgatatt catttttgtt aaattgttta tcttattcga acacacgaaa
    52741 caaacgttgc atgatccaat catcgcctcc gtttcaccaa cttttacgat gtcactcatg
    52801 gttatttgta cttattttgt ttcatatgaa agtattgcac caattgtcaa attcatttgg
    52861 ctgtgcgctg ttatcttcca agtgatcctt gtcttttatt tcaattatca ccatgttatc
    52921 aaagctgatc tttcaattga agcaatttat ccaagctggt ttatcattta cgttggattt
    52981 ggggtcatca ctgtaactgc cgggaacttc ttcccactta ttggaaaaat tttcttttgg
    53041 atttcattga tctgctatgc gttcttattg ccgatcatca tcaaacgtgt ctttttcgta
    53101 agaaatatgg ctcatccgac acgtccgctg attacgatcg ttgcagcacc tggttctctt
    53161 tgcctaacag gttatcttaa aaactttgct gaacctaatt tttatcttgt cgtagcattg
    53221 ttcgctttat ctcaagtatt gtatttcgtt gtgctgtcta tgcttccacg tttgttcaag
    53281 cttcgttttt atccaagcta tgcagccttt acattcccgc ttgttatctc tgcaacagct
    53341 atatttacta ctgttcatta cttcaacagt ttaggtatct atagtagcgt attggatttc
    53401 ttaaaagttg cggaatgtct gtttgcaagt gtagttgtct tctacgttct tgctcattac
    53461 gtacgtttct taattaaaga acatagcaaa catggaaaat ttgtttctaa aaaaatcgct
    53521 taataatcga ttcaataggt tgtgaaaaag ctgttctgca tcaagtaata agaacggcca
    53581 aacaaaaaca gctgttcatg tttttgtact ggttgggctt attaccgcag atgcaagctt
    53641 ttgaacaccg tttaccaaga ggtcgtaaaa gaagtggtca gcttcaagaa ataagaagga
    53701 atttacgaaa attgcttctc aaatttttgt gaaatttcgg cttatttccg tagaagctac
    53761 ttctggaaca ccgtttattc gaaacaagag gtcgtacaag acttttgtca cgacctcttg
    53821 ttcttttgtc aaaatactgg tttagtgcta ctatgttcga gtatgctttt agtatttatt
    53881 gacgaaagga accgatcaaa tgaaatcatt gtcacatcca ataaaatctg taaaggattc
    53941 agcggtccgc gaaatcttac ccggtttact gctttctttt gccatcgctg ccttcagcag
    54001 gcttcttgct atttatctgc cacagttagg tggagcgact attgctattt tattgggtat
    54061 tttattagga aatacttttt ttaaacagcc tgttctagca atcggcacaa aatttgctga
    54121 aagcaaacta ttagaattct ctgttttttt actaggtgca actgtcacag ctcaaacgat
    54181 tgctcaaatt ggtgtaaaag gaattgtttt tgtgttactg caaatgtcag tcactatcat
    54241 cggtgcttac ttgattggta aaaaactgca attcagcgat acttcttctt tgatgatggc
    54301 tggaggaaac gctgtttgtg gttcttctgc tattgcttca atcgcccctt ccatccatgc
    54361 gaaagaggaa gaaaaaggac agatcatcac actagtcaat ctactgggta ctgtgatgat
    54421 gttgactctc ccttttcttg gattacagtt gtttggtgga aatctcattg aaaaaagtgc
    54481 acttttgggt ggtacactcc aatctgttgg tcaagtagtc gctggggcaa gcttacttga
    54541 caattctgtt gtcgaattcg ccatgctatt taagattcta cgtattatga tgctcgttgt
    54601 tgttgtctat ctgtttgaaa aattcatgca caagaaacaa ccagctctgt ttgaaacgcc
    54661 aactgatacg cagaaaaaac gaaaattacc gcttccttgg tatgtattag ggtttgtgat
    54721 cttttgtttg gtcaacagct tgttttcttt acctgtagtt attgaccaat cggcacattt
    54781 tatcagcagc tggtttgaga tcactgcttt agcggcaatt ggtctgcgtt tagactttgc
    54841 aaaattcatg aaagaaggta cacgcttctt atcttatgga ttagctgtcg ggcttctcca
    54901 aacagtgatg gctttactat ttatttggct gatcaaagtt tagaatgaac atgaaaaaac
    54961 agttgtgatt ctcgcatgga acaatgtccg aatcacaact gtttttaatt ttgcagatat
    55021 ccttctaaca acaacaattg gcgtttgatt tcaaccccat ctattgcgtt tcccatatat
    55081 cctgtcagtt ttccattttg cccgatcact cgatgacaag gaataacgat tggcaatgga
    55141 ttggttcgat ttgcctgccc aatcgttcgc acagctttcg gactatcaac agcaacggct
    55201 acttctttat acgttcggat ttctccataa ggaatggtcg acaaagcttt ccagacattt
    55261 tgctgaaaag gcgtacctct ctttatagat aaaggaacat caaattcttt tctttttccc
    55321 gcaaaatatt cttcaaactg ttcttgtgtg cgcgttaaaa ttcgctgatt cgctgattcg
    55381 ctttctgaaa ttggctgaaa agatacagca gtcaatccgg attcatctgc atctaaccat
    55441 aaaaagccga taggtgtgtg tattttcatt ttgtttgctc ctagtcaata gactggaact
    55501 tcctcctttt tctctatcca aaactacttg ccaactaata aaaattgttc ttaaaagttt
    55561 gataaaccga ggaaacatgg taaactaaat acgttagttt acaaaaagaa tacctgttct
    55621 tttacgtaaa atcaaatttt aaaggtgtta tttaaggttt attaaatttt gaatgtaaac
    55681 catatataaa catacgattg aaaggataga aacgatgaaa gtatcaattg ttgtcccatg
    55741 ttttaatgaa gaaaaaactg ttcctctttt ttttgatgag gttgaaaaat tcaaaggtgc
    55801 ccatgagttc gaatatttgt ttattgacga cggatcaaaa gacggtacgt tagcagcaat
    55861 caagaagcta gcctcagctt atccttatgt ccgctttgtc tcattttcca gaaattttgg
    55921 gaaagaagcg gctctgtacg ctggattgca agcttctaca ggagatttag tgaccgtgat
    55981 ggatgttgat ctgcaagatc cgcccgaact gcttcctgag atgatcaacc ggatcactac
    56041 ttctgatatc gattgtgtcg gcacccggcg ttcgactcgt gaaggcgagc ctgcgatacg
    56101 cagtttcttt gcgaaaaaat tctaccagtt gatcaataaa atcagcgata cagaaatggt
    56161 cgatggcgct cgtgatttcc gcttgatgac tcgtcaaatg gtggatgccg tcctagaatt
    56221 aacagaatac aaccgatttt caaaaggatt atttagctgg gtcggattta aaacagaata
    56281 tatttcttat gaaaaccgcg aacgtgtggc tggcgaaaca tcttggtcct tctggaaatt
    56341 attcaactac tcgattgatg gtatcgtgaa cttctcggat gcaccgttaa cgattgcttc
    56401 atttatcggc gctttgtctt gtgtcggttc tggtattgca attttgttca ttattttgcg
    56461 ggcattgttg tttggtgatc ctacatcagg atggccttcc atggtctcga tcttcctgtt
    56521 cattggtgga atccaattgt tatgtcttgg aatcatcggg aaatatattg ggaaaatctt
    56581 cttagaaacg aaacagcgtc ctgtttatat cataagagaa acagaaaaag tgaagccaaa
    56641 tgaattgaag cctcaccaat aatatacgtc aaatgaagta aacatttttt taaatgaaaa
    56701 aggactgtga caaaagtctt gtcacagtcc ttttttccga ataaatggtg ttccagaagt
    56761 agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc
    56821 cttcttattt cttgaagctg accacttcta tcacaacctc tttcatatct tattacttct
    56881 tcattgctgc ttttagtgct gctgctaatg ggctttcttc tggttcttct gcttgtgcat
    56941 attttttcag caagcgtcgt tcttcatgtt tggtcatttt cttcttctgc tcttttttat
    57001 caagcaattt ttctgtcgtt ccatcgtatt tacaacggaa ataagcaccg tttttacctt
    57061 cgatgatttc cattttccgg tgacattgcg ggcatcgatg attagagact tttggatctt
    57121 ttcttctgcg gtagttgcaa tctgaattgg tgcagacata gattttgccg tcacgtgtgt
    57181 ttttctcgcg cagattggcc ccacattctg ggcatttctt ttgggtaatc gagaaatctt
    57241 gatattcttg tttgcttgat ttgatctcat tcaccaagcg ttttgtttct ttttcgattc
    57301 cattcaagaa ggttcctgct tgtttctttc ctgcagcaat agcttcaagg tctttttccc
    57361 agctttctgt caaatctggt gttactaaag agggatttac caatactaat aattgcttac
    57421 ctttaggaga tacttgcagt ccttgagggg tacgttccat cagttcagat ttgattagtt
    57481 tctcgataat ctctgcacgc gtagctgggg ttcccagact gtatttttcc atttgaccaa
    57541 gcaagcttgc ttctgacaat ggtttaggcg gtgcagatag ttcttttttg atggcgaaat
    57601 cgggattgat tctcattcct ttttcccatt tgactgtttc cgtctcagtt tcagtttcgc
    57661 ctttccatcc tgcttggacg acacgatttt gccgaaaaat gaatcgttct ttaccaaatt
    57721 caaccgtaac ttttgtttgg ctgactttat ggggttgagc aaacagtcct aaaaaacgag
    57781 agacaatcat ttgatagatt tttcgctctt cattacttaa tttttcataa cgtgctctgt
    57841 tttcagttgg caaaagtgca tggtgatcgg tcactttact gtcttggaat acttttgtct
    57901 gttggacgat ggctccgttt ttcaaatact gcttgacctc aggagcaaag tcgctgactg
    57961 cttgcaatcg ttctttcatc gtacttttca tgtcatttgt cagatatttg ctgtctgtac
    58021 gcggataagt aacgatctta tgggtctcat acaaactttg tacaagactt aatgttttct
    58081 ttgcagaata gccatagcgt tgattggctt ctctttggat ctctgttaaa tcataaggca
    58141 atggcgcaga ttccgttttt aatttttctt ggacttctgt gacagttcct ttgcttgtac
    58201 tgatcaattg aacaagtttt tgtgcttctt cttgtgtttt taaagcaaat tgattttttt
    58261 gaagcatttt ggcttgttct tttccagctt gcaacgtgat cgtgaaatac gtttgtggac
    58321 ggaactgctc gattttcttt tcttggtctc tcactaatgc tagtgtcggt gtctgcacac
    58381 ggcctgctga taaattatcc gcgtacttca cagttaaggc tctggtcaca ttcaatccta
    58441 ccagccagtc cgcttttgcc cgggcaagag cagattcata caatgcatca tattctttcg
    58501 caggtcttag ttgcttgaat ccagttttga ttgctttatc tgtttgagaa gagatccata
    58561 accgcttcac tggtttattg aaatgcacat actctaagat ccagcgagca acaagttccc
    58621 cttctcttcc ggcatctgtc gcaatgactg cttctgagac atcttttcgc tgagccagct
    58681 gcttgattgc ttttaattga tgtcctgttt taggaagtgg tttgattcct agttttttag
    58741 ggatcattgg caacgtgtcc atttgccagc tctgccattc cttattcaga tcttccggca
    58801 ttttcaatcc taataaatga ccaagggccc aagtaacgat caccttgggt ccttcataat
    58861 aatttttgtg cttttgatta gcaccaagca ccttgcttag gtctttcgca acgcttggtt
    58921 tttccgctag aattagttgt ttcataaaat tcctttctgc tttctactgt gatgggctga
    58981 tgatttttca acaacgcaag tcccttgtat tgctgtaaat catcgtcaca ctcttcacac
    59041 cagcgctcat ctccgtcatt ccaaaaggcg ctgagaaagt tactttttgc attgatatat
    59101 tcataatttt gatggatttc gttccattgt ttttcgtacg cttgtcgtgc ttcttcgaaa
    59161 gactcgaatg tttcttcttc ttcaatatct tcttcccagt cttcaaaaaa ccaccaaggc
    59221 tcattgtccc cgaacatggt aattacttga tacatactcg tgctccttct ttcagctttt
    59281 ctagactcat cttagtcact tttcaagaaa gttgcaagaa ttttctctta taaaagggtc
    59341 atttaatgaa catcttaaaa ttgttacaaa tgaatctgat tgtttaaggc aaagtagcga
    59401 taaagttatt gatcatccaa aaatagctgt cttctggatc gattgttggt tcttttgtaa
    59461 agtatcctaa aaaacgtaag gaaagataac cgtggatcat actacgaagt tccctgcttc
    59521 gatgaacttt ttcttctttc gttaattgaa aagcctcaaa tatctgcaaa atgatctgat
    59581 tcgtttcatg gattccatct aataaaacag cttcattggt atgcggtaca ctgatcagta
    59641 actcatacac tgcttgattt gaaaaagcaa aggatcgata agtttctgca tagatctgca
    59701 gtgcttcctg accacttttt cccacaagag cccgtcgcag ctggtcatta agttcctgca
    59761 gaagtttcgc tgttagcgct gttttgacat cacgaatatt tttgaaatgg ttatacagcg
    59821 agggagcctt gatatccaag cgtttggcta aatgctgaaa ggttatttgc tgaacatcca
    59881 tctcttctgc tagttctcga aagcagtcga tgattttctc ttgagacaga tttctttttt
    59941 tcatttccat gtttcctctc ataaaataaa tccttcactc tttcagttta ccacgaaaaa
    60001 gaaagagaaa aaagagactt tcattgataa actacatatt gtagtttata ataaagagct
    60061 gtagatgaaa attttcagaa agaaggagtc taattggctg caagaataaa ggaaaaatta
    60121 tttaggaaaa gagaaacttg ggaacaaaac ttgtttgtct tatggtttgg aacatttatg
    60181 gctggtatcg gatttagttt agtcatgcct tttatgtcac tttacataga tacgttaggt
    60241 aattatagta cgtcacaatt gaacttttgg agcggattga ctttttcatc tactttcttg
    60301 gtgactacgc ttatctctcc ttggtgggga aaattagccg accaaaaagg aagaaaattg
    60361 atgctgttgc gtgcttcatt aggaatggca atcgtcattt ctttgatggg ttgcgtgact
    60421 agtgtgtatc aattagtcgc attgcgtctg cttcaaggga ttttttctgg ttatatcagt
    60481 aatgccactg cccttgtcgc aactgggacg ccaaaagaaa aaagtggtca agttcttgga
    60541 accttagcaa ctggttctgt tacaggaaca ttacttggac cgcttcttgg cgggatctct
    60601 gcttcagcgt ttggctatcg ccctaccttt ttcattacag ggatcatctt atttttcgtc
    60661 ttcttgttga gtctgttttt cgtccatgaa aaatttgtcc cagtgaaaaa agaaaacatg
    60721 gtttctgcca aacagatttt caaagacttg aaatatccac acgtcgtcat cggtatgttc
    60781 gtcacaacga tgatcatcca agcatccaac aattccatta gtccaatcat cagtctgtac
    60841 attcgccaat tgctgcatgg acatgggaat gttacgttga tcagcggaat cattgcttcg
    60901 atacctggga ttgccacttt gatagccgct ccaagatttg gtcgcttagg tgacaagatt
    60961 gggagtgaaa gaatacttgc gattggtttg ggttttgcga ttctggttta tatcccaatg
    61021 gcattcgtca cgaatgtttg gcaattagct gccttacgtt ttttgattgg tatctctgat
    61081 gcttgtctgc tccctgctgt ccaagcattg atcacaaaat attcgcctca tcaagctgct
    61141 ggacggatct tcagttataa ccaatctttc caagcaactg gtaacgtagt agggcctatg
    61201 atcggctcca gtgtttccag tgcatttggt tatcgtggcg tctttctttc gacttctctt
    61261 cttgttttgg tcaacttctt gcttgtacga cacaatacac aagagatcca tcagcaagaa
    61321 actgcagaag aaaaagcttc aggagtctcc atagcttcag ggcactctcc ttctcataga
    61381 caataagcgt aaatgaaaaa cactgcttga acagttgtca cagactgtcc ttgcagtgtt
    61441 tttgtttagg cctgtttttt caagaactca gataagagga ataatccctt ttccaaggtt
    61501 tcttttttac aacagtaacc tagtcgggca tgcttcggta aatcaaaggc acttcctggt
    61561 actaatagta ctcctgtttc ttctaataaa ttgatacaaa atgtctggtc atcttcttcg
    61621 atggctaatt taatgaaaga agtagagacg taattgggag cgacaagttc aactttcggt
    61681 tcattggcta cccattgttt taaaatcaca aggttctctg taatgatctt tttgtttcgc
    61741 gccaatattt tttctttatt cttcaatgca tgtacagcaa gatcatctga caggactccg
    61801 ccgcaaatca tcgtgtagtc tcgatacttt cgaaaaactt ctgccaactc aggtccagta
    61861 gctgtccagc caatacgaat acctggaatg gagtaggttt tggataggga attggttgct
    61921 ataccttttt catagacatc tacgatcgac aagaattcac ctttatccgt taaaggcgca
    61981 tatacttcat ctatcaatac atatgcatct actgtacgag caatttctgc gattttttct
    62041 aaagttttcc gatctagcaa tgtgcctgta ggattgtttg cactattcaa acagatgatt
    62101 tttgtttcag gtttgactaa ggcttctagc tgttctaaat caaactgcca atcttcttct
    62161 tccttcaaat gaacaaagtc aacagcggca cctaaagatt tggggatgtc atataattgc
    62221 tgataagtag gaagcatcga aacaacatgg tctcctgctt ccactaacgc gtatagagct
    62281 aaaagattag caccagtcgc tccattcgtt tgtaagatgt tttccggatc gacggtttga
    62341 taaagagaag cgacttcttg tttgaaaaca tctgatcctt cgatccagcc atagtccaat
    62401 ggttcgtttt tgcgtttttc gaaaaattta cttacgctag tcccatctaa gccaacgagt
    62461 tcgtctaatg tcaatgcttc gatcgagctt tgtgcaatat catataccgc tttcttttcc
    62521 catttattta gccattcttc tacaccgaaa tttgcgattt tcatgtttct ttccccctta
    62581 tatcttgatc cagcctacag caagcaaccc gattgaaagc agtgcggcca cacaaatcag
    62641 cccaatcatt actttttctt ttcccgtaat ttgatgtttg gtttcacgac aagcgatcca
    62701 gtagaagcaa aaaccaggaa gataggcaat actacataaa aggacataag aaaatcctgc
    62761 taaaatgatg cccatcaatt caaaaactac agcaatccca ccgatcacta cttgtttttt
    62821 gattccttct tttaaagaaa actggacttg atagatcccg acaagtagat aacaaatcaa
    62881 aattgccgct gtacataaag aataggcaaa ttgataggca taatcagtaa ctaagaaagt
    62941 caataagaat agattagtca agattgcagt aacaactaac gaataagttg gggcattttt
    63001 tttattcgtt tttccccaac ctttcggcaa cagaccgtct cgagccatca acatcgttgt
    63061 ttctgctggg agcatcgtcc aagacaacca agaaccaatg atcgaaataa tcaagccgat
    63121 attgatcagc gctgctcccc aatttcccac gattcctttt aaaatatttg ccatagcagg
    63181 ttgtgaaata gttgctaact cttcttgaga taagacgccg tatggcaaga tcgaagcaag
    63241 gatatagata aacaataaac aaagcagacc aatgatcgat gcttgctggg cttctgaacg
    63301 tttcttcgcg cggttggcta aaacacttgc gccttcgatc cctacaaaga cccacatcat
    63361 aaccatcaaa caacctttaa tctgcaacca gatcgttcct tgctgccctg ttccattcac
    63421 tagattatca gatacgttac cccaaaaatc agcagtgaat atattcactt taaatgcgat
    63481 aaatacaaat attaaaaata ggaataatgg cactaattta cagatcgtca cgattgtatt
    63541 gatgacgctc gctccttcaa ctccattgtt tactaataaa gtcagacccc aaataaaaat
    63601 acttgcaaat aaaatagaag gaacattttg tccacctttg aatatcggga aaaattctcc
    63661 catggcgctc atcagcatcg ttgcaaaagc aacatttccc agccacgcag acaaccagta
    63721 accccaacca ctgatgaagc cacctaattt accaaatgaa gcttccgcgt aaccgaaaat
    63781 accaccatct agatctggac gcttttctcc taaatgattc agcgataata ccaaagctaa
    63841 aataccaatc ccaacgatta tccatgaaag gatcgctggc cctggagctg cagaactagc
    63901 cagatcactt gtgatcccaa acacaccgct tccgattgac gaactgacaa ccagagcaac
    63961 cagtccccat ttgctaatac ccgtcgattt cttttccatt tccatcaccc tcacttataa
    64021 aattgaacaa atcacttgca aaagattccc ttctattttg caactgctct tgcttaacaa
    64081 aaagcaactt tattcatatt attggatatt atgaactttt tacacaagta tttcaagtat
    64141 aaaatgaata aaaattaatt ttattgcaaa aaaacagcta tgctatccaa aagaaaaggt
    64201 gtttcttttg ttagccatag ctgttttgat tcatttaata tcgcttaaac gtattgtaaa
    64261 acttttgttt cttgatatgc gtggtcgatc aaaccaccac ccagacattc cattccatca
    64321 taaaatacaa ctgcttgtcc aggagtgatt gcacgtacag gttcgtcgaa tacgacttct
    64381 gcacgattat catccaataa gcgaactgtc acaggaacgt cttgctgacg ataacggaat
    64441 ttcgctgtac acttgaattc ttttggcatt ggttcattcg tcgtaaagtg gatttcactt
    64501 gcatccaagc ttgtggcata caatgcagga tgatggaacc cttgccccac atataatgtg
    64561 tttgtcgcta gatcttttcc tacaacaaac cagggttctt gcgaatctcc tccgccaccg
    64621 atccctagtc cttgacgttg accaatcgta taatacatca agccagcatg ttgtcctttg
    64681 acttcaccat caagagtgac catatttcct ttttttgctg gcagatagtt gctcaaaaat
    64741 tgtttgaagt tcttttcacc aataaagcag attcccgttg aatctttttt cttagcagtc
    64801 gcaaggcctg cgcgttcagc aattgcccgt acttctgatt tttccatgcc gcctagaggg
    64861 aacatcgttt ttgaaagctg ttcttgtgat aattggctaa ggaagtaagt ttgatcttta
    64921 ttattgtcta ctccacgaag catgtggacc acgccgtttt catcgcgagt gacttgcgca
    64981 taatgtccag tagctacata atcggcgcca agatccattg cataatccaa gaatgctttg
    65041 aatttgattt ctttgttgca catcacgtct gggttaggtg tacgtcctgc gcggtattcg
    65101 gctaggaaat attcaaatac acgatcccaa tattcttttt caaaattcac agagtaataa
    65161 ggaatcccga tttgagcagc tactttcgct acatccttat aatcttccgt ggccgtgcat
    65221 acaccgtttt cgtccgtgtc atcccaattt ttcatgaata ttccgatcac gtcgtatcct
    65281 tgttccttta acaaaagtgc tgttacagat gagtcgacac cgccgctcat ccctacgaca
    65341 acgcgtgttt tgctgttgtc tgtcatgata tcaccatcat ttcaattaga ttctgaaaca
    65401 tctacgagtt gaaggtcgca acttttcagt attttacatt atacgtcata tttctgaaaa
    65461 tttcatccct taattatcaa gtcgtaaaaa tactgttcta ctggacctat tcagtacaca
    65521 aacatgatag tcaaccaaaa aatcactcat cttttgagca ttcgatataa aaaattggca
    65581 tactcataaa aagggccagc ctttcggcaa taaggaataa ttttataaat tgtgaaaaac
    65641 tatttttcaa aattgcttct tatcgcttaa agctgcacgt ctcatttaca accttttctt
    65701 tttagatgtt atgagttgct cagataagtg attttgttta ctctaagaaa aaagggaggc
    65761 aaaagataca tgaattactt tatcagtgat atgcactttt tccatgaaca cttattagga
    65821 aaaaacgact ttgctccgcg gctctttgca aatgtggatg aaatgaacca acagttgatc
    65881 actagctgga atgcagtaat aaaagaaacc gaccatgttt atcatctagg cgaccttgct
    65941 atgcatccgc aatatgaaaa agcaaatgca gaaattttgg aactggtcac tcaactgcaa
    66001 ggaacaatcc acttgatcaa agggaaccat gacagtcgcg cttttttccg ttatttagaa
    66061 aaaaatgatc ctaggcttac tgacggaagt cccaaatttt atttttatga tgtaggtgcc
    66121 atcgttaaat tcgaccatca acaatattac ttgactcatt atccgatgtt gctcgggcct
    66181 aacgacacta tccgcaatct tcatggacat atccatcatt acagcgtacc tatcgagaac
    66241 gatgtcaatg taggcgtaga cgcaccggaa agagaactct taaaggaaaa actaccattt
    66301 ggcactccgt tatctgagca acaaatcaaa gaaatctatg aagcaaagag gaagtacgat
    66361 aggtcgtgaa aaaactgccc tatatcaagt attaaacaaa taaagataat tactcatatt
    66421 tttcatttga gtaggcttat tgccacagga acattaggat cagaaatgga gttgtaacaa
    66481 gacttctgtc acaactccat tccatactat tcgtcttttt gctcatggtg ttcttttacc
    66541 agttttttct gtaattcttt atcataattt cgggcagctg acgttaaaaa taaaacgatt
    66601 ccgcataata aaatcccgat tccggaaaac aagaacattt tttctacccc taaacgatca
    66661 gctaatggtc ctgcaaataa taatccaatt ggacctgcca ggttcatcaa agagttgaag
    66721 ttccctaaga cacgacctaa aacattcgat tcataactct gctggatcat tgccatcagc
    66781 agtgtattga aatatggcgt tgcacaacct gctcctgcat tcaagataag aaagtacaag
    66841 aacccttgac tatcgtttgg aaccaatccg cttgcaccga tcgtcaggcc cataatgatg
    66901 taggctataa taataggttt cattcggtct ttccatgttc ctataaaact gatcagtgca
    66961 ccgcctaaga gcattccgac tgcgtagact acttccactg ctcccgcctg tccaacggaa
    67021 ccaccaaagt aatctagcgt catcaatgga tacagactga ttgctggcat gaacaacagc
    67081 atcacaaatg caccattgat cgttatgtac cataaccctt tattatccat caattgctgc
    67141 agcccaaatt ttgcatcttt aaaaatagta agcggaactt ccaatatttc aggagaaact
    67201 tttgggatct tcacaaaaat caataagccg acaccaaaaa ctgcccccaa tacatctaat
    67261 aaaataaggt aattgacagg aacgactgaa aacaaagccg cacctagagc tggcgcaatg
    67321 atgaaattcg ctgattggac cattcctaac tgtccattcg tccttgttat gtgagaagat
    67381 ggaacgattg tcggcataat cgactggatc gttggcattt ggaatgtttg tgcgaccgaa
    67441 cgcataaaca aagacacaaa aactagccaa atcgggaagg attctgaaat cgttccgact
    67501 actgctaaaa taagcgcgaa aatcgctaca ataatatccg tgacaatcaa aagagctttt
    67561 ttatcccagc gatccactag cggtcctaca aatgggctta gtaaaaccat gggaatcatt
    67621 cctagtaaag tagcaaaact caaaattgtc gccgaaccgg tttctcttgt caaataccag
    67681 atgatcgcat attggacgac catactagtg attcctgaca aaaactgtcc cgataaaaag
    67741 aacaaataat ttctcttcca attcgttaaa acgaccgact gttcattttc cataaacatc
    67801 cctcttttct ttttcatttt gtatagcgtt tgcacaacaa tcattatacg ggattctttt
    67861 ttcaaaaaat acctttttcc ctaaataaca atcgtttcaa tttaaatttc tttacttaga
    67921 aacaataaat cagcaaaata ttaaaaataa caacatgtgt taaatttaaa aataaaaaag
    67981 acaactaatt tgcgtcgtcc ttacaaagtt agtcatctaa caataggccg tttcaaaact
    68041 agttgtcttt ttatatccac agatcattcg actttttcga ataacccacg gctaatcatc
    68101 ccatccatca gaaaataaaa tttatcttgg ctcattttct gttttggatc tttgaaaata
    68161 ttgactgctt tcaatccatt tgctgtagtg atcgacattt tcaatgtttg aatatctttt
    68221 gatacagtga tttttaattt gaatccttct ttgctttgcg gcgtggcatc taatggtcgg
    68281 atcaattgat aattgcctcc gtttgtttct gtaaatccat tatcgcgcaa tgtatatctt
    68341 tttgcatcag gatgcaaacg atagctaatt ggtgcgccca gtaccgttgc tgttgtttca
    68401 aatgccatga ttttcacctc gataaaatta gtttctcttt attataaaag tttatcctga
    68461 tttttactag gtttttcagt taagttccct aaaaaatgaa ggcgttctct cagacatctg
    68521 ttcatataag aatgacttca ctagttattt tttgacagca cgaactaatt gatcaaggaa
    68581 atactggatg tcgtcttcgg tatttcccaa accaaagctg atacggatgg attcttttag
    68641 tatcggtgta tcttctccat acattgcttc taatacgtgt gaaggctcga tatttcctgc
    68701 tgtacaagct gaaccagtag aaatagcgac cccattaaga tcaagcttca tcagtagcaa
    68761 atcatttgct tcgccaatta agcgaagatt caacacatga gggagtttat tcgttaaatc
    68821 accatttact tgataaggga tttcgtttgc ttctaatgta tcaagaatca attgctggaa
    68881 tcctttatat ttgttgttac gtgcttcacg ttcttcaggt gacagcagtt caactgcttt
    68941 tgccataccc cagatccctg ctaaattttc tgtacctgca cgccgctttt cttcctgctc
    69001 cccgcctaaa agcaatgcag ggatatttac tccgtctcgt ttatataaga aaccgacgcc
    69061 ttttggccca ttgattttat gagcagagat acttaataaa tcgattccta attcttctgg
    69121 atggatcgtt tggtttccgt acgcttgtac cgcatctgta tggaaataag ctggatgatc
    69181 cgctaaaagt tcaccgattt ctttgattgg catcaaattt ccgatttcat tgttcccgta
    69241 catcacagag accaagatcg tatcttctcg taacgctttt ttgacctctt cgatcgacag
    69301 attccctttt tcatctacag gcagatatgt aacgtcaaac ccctttgtct ctaaatattt
    69361 catggtatta agaacagcag gatgttcgat agctgtagtg attagatgtc tgccttcttt
    69421 ttgacgcgag aacgctgttt ctaagatagc tgtattatcc ccttctgttc ccccactgtt
    69481 aaagatgatc tcgtgaggtt ttgcttgcag actgtctgca atgatttgtc ttgctgtttc
    69541 caatttttca tgcgcgtggc gaccaaatcc atgtatgctt gaaggatttc caaacgtgtc
    69601 ttgcatgatt gccgtcatcg cttcaattac ttgtggatgc atgggggtag ttgccgcatg
    69661 atccagataa atagttttca cttcattcat cccttccaaa ttattgctag gcacattcta
    69721 gcataaaata gcgaacaaac caaagaagag ctcatttttg tctagaggac gtttcatggg
    69781 catagaaaaa aggagctgtg acaaaagtct tgtcgcagct ccttgaagct gctcacttct
    69841 gtcacaactt ctttccctct tttttttgtt tattcgaatt ctttttcttc cagacggaat
    69901 aacggactca tcggtgtatt ttcatggatg cgtttgaccg cttcacccat taattctgaa
    69961 catgtaacga ttgttaagtt ttctggatga cgattttctt ctgtgaaaac agaatcagtg
    70021 atacaaatat ctttgattgg tgcattgtct aaaatacttt tcgcatgatc ggacaataat
    70081 ccatgtgacg cacaggcata tacttctttt gcgccgtttt cttttaatac atttgctgct
    70141 cgtgcaagcg ttgctcctgt gttcaagata tcatctacca tgatacatgt ctttccttga
    70201 acatcaccga tcacataacc accctcagtt gaagcatcat ctgcatgatc tacgatggct
    70261 aatgtgctgt tcaaatattc tgaaagacta cgtgcacgct gaacaccgct gttttttggc
    70321 gaaacaacta cgatgtcatc tcccgtcaga cctaattgac ggtaataatg agcaaacaac
    70381 ggcatcgtaa ataaattatc tacaggaata tcaaagaatc cttgtacttg aaccgtgtgc
    70441 agatctaaag tcagtactcg agttgcccct gcttcttcga tcaagttagc gatcaatttc
    70501 gcagtgatcg gttcatgagg tttcgccgtg cggtcttgcc tagcataccc atagtaaggc
    70561 aatacgacat tgatcgtttt cgcacttgct cgcttcattg catcgatcat gatcagtagt
    70621 tccatatagt aatcattgac aggtgcattc gttgattgaa cgatatacac atgatcccct
    70681 cgaatacttt cttcgatatt gatggaaatc tcgccatcac taaattgctt cacgacgctt
    70741 ttacctaatt ctgttccgaa cactttcgcg attttttcag ctaatggacg gttgccattc
    70801 aaactaaaaa tcttcaacgt gtcatcctga tacttattac tcatgtagtc ctccccaaaa
    70861 cttctctatt cttcgatagt ttaacatatt tttccaagat tttctgttaa tttcagcaaa
    70921 ttcttaagga agtgaaactg ttccgttact ccaactggca ggaatgactt tatgataatc
    70981 gccccagttg tcgtgtactt tgtaataacg ttctcctgtt tcagtttcaa aataagcgta
    71041 cgctgtcacc caatgattgc catatgtact gccaagtact ttcaaaatac caatcatcac
    71101 aggtttgcct tcacgtatac gttttgtagc tcgctgccat gaacctagaa gagttgctcg
    71161 tgcacgctca tgattgccat tcttctttaa aaaactagaa atccctgtac taacctgaaa
    71221 aggaatcgta ggaagtccta gtggttggat ctggctacgc aatgccttcg tcaaaaccaa
    71281 cgaatcagcc gtatttttct tgcgaatctc atttggaagc atccacccct tacgatagtc
    71341 ttgataatag gcaaggagaa ctgctgccgc gtaagtacca caaagatagc ctgagggggt
    71401 agcccatttc ttatacctca aaaaacgttc ttctggcaat cccacccagc aatttgcgag
    71461 ttcctttcct gacctttttt cttcgatcaa aacgggaata tcttcttttc tgttgatcca
    71521 tctagataac tcgattggct cttcattgat cagcttccat tttttacaag aaagaccctc
    71581 ttgataagta tattttgctg gagaatattc taccggatgt cctgattgat cgataatcac
    71641 ataactttga tcttcaaaaa ccagagctaa aaaaagcaat ttactttttc ttgactgcag
    71701 ctgctgagtg ctggctaatt ttttaatttt tttatctact ttgaaatata aaggtttatc
    71761 cactacttga ttcagttggg atcgcctcct ttttttcatt ataagtgaag gattgaaaaa
    71821 aacaaatcag tttgattgtt cgaattttca attttttatg ttctatttgt taattccttc
    71881 attttgtaag ataatgggca cacgaaacaa aaaaggagcg atcgatgaat gaacaaagaa
    71941 cgattgaaac aagatttcta tgaagcagtc aatgaagaat ggcaaaaaac agcaaaaatc
    72001 cctgcagata aaccagctac aggcggtttc caagatttag ttgacgggat cgaccaactt
    72061 ttgatgggag aaacagacga tctgcttgcc catccagaaa aagtgtcttc agaaaaaatg
    72121 aaacatttcc ttgcatatta tcaactggca aacgattatg accgaagaaa tgagttgcat
    72181 gctcaaccgc ttcaacctat aatcgaaaaa gttgaatcat tgcagtcttt cgacgaattg
    72241 aataagcaat ttccagaatg gctgttggat acattaccat tgccatttaa tctggatgta
    72301 gatgcagata tgaagaatgc gcaaacgaac gccttctttg cttatccgcc ttctttattc
    72361 cttccagata agacgtatta tgcatcagac catcccaacg gtgcacaact aataggcgtt
    72421 ttctttgata tggctgttca gttattagag cttgccggca aagaaaaaag tcaggcagaa
    72481 gcaatcacgg aacaagcagt gcaatttgat aaattgattg ctcctcatgt aaaaagtgca
    72541 gaagaaaacg cagattacag caaaatgtat aatccgcaat cttttgatac atttattaaa
    72601 tacacagata aactggactt gaaaaaactt gtcacgggtt tgattgggac aactccagat
    72661 aaagtgatcg taactgatcc agtgtacttc gatcatttga atgacatcct gacagaagct
    72721 cattttccgt tattcaaaag ctggatgatc attaaaacag tacgttctct tagtggttat
    72781 ctatcagaag aattccgcca agtcagcgga atctattctc gcgtattgtc tggaacagac
    72841 gaagcaatgc ctcagaaaaa agcagcgtat taccttgcct ctggacaatt cgatcaagta
    72901 gttggcgatt actacggaaa aaaatatttt ggcgaagctg ctaaaaaaga cgtagaacaa
    72961 atggtgaaaa aaatgatcgc cgtttataaa aaacgtctag aagaaaatga ctggcttggg
    73021 gatgcaacaa aagaaaaagc cgtcataaaa ttaaataact taggtatcca agtaggctac
    73081 cccgataaga tcccagcatt attcgaccag ttcaaaacag tacctgcaga tcagggtggt
    73141 actttgcttt caaatactct tgttttcagc aaactcacat taaaagatcg tttcagcaaa
    73201 tggaacaaac cagtggatcg ttcagaatgg gaaatgagtg ctgacacggt caatgcttat
    73261 tatcatccat ttcggaatgt gatcgtcttt ccggcagcga tccttcaagc accattttac
    73321 agtctagaac aatcaagctc tgctaattat ggtggaatcg gagctgttat cgctcatgag
    73381 atttcccatg catttgataa taacggcgct ttatttgacg aatatggcaa cttgaacaac
    73441 tggtggacag aagaagatct tacacaattt caaaacaaag cacaagcaat gatcgaagaa
    73501 ttcgatggtc tgccttttgc tgacggaaaa gtaaacggaa aattaaccgt ttcggaaaat
    73561 attgctgatg caggcggtct aagctgtgca ttggaagcag caaaaacgga atcagatctt
    73621 tctatcgagg aattctttgt caattgggca acgatttggc gtacaaaagc aaaaaaagaa
    73681 taccaacaac ttttactgca aatcgatgtc catgcccctg ctaaactccg agcaaacgtg
    73741 caaccaaaaa accttgacga attctatgaa acatttggta tcacggaaca agacgctatg
    73801 tatctagcgc cagaaaaaag agtgcgaatc tggtaagagg ttgtgccaga agtggtcagc
    73861 ttcaagaaat aagaaggaat ttgggaaaat cgcttctcaa atttttgtga catttcggct
    73921 tatttttcga agaagttact tctggaatac catgtattag aggttgtgaa aaagctgctt
    73981 agtttcaagt agtaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg
    74041 acttactacc gagaaactgg cttttgaaca ccgtttatcg agaggttgtg aaatcagcgt
    74101 cctgacctga gtattagaga aaaaaacgga aaaatagttc ctcatatttt gccgtttgtt
    74161 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagagattg tgaaaaagct
    74221 gtcctgcatc aagtaataag aacagccaat caaaagcagc aaggagaacc tcatctataa
    74281 aaagatagaa ggaagtgaga catctattgg tctcatttcc ttctattttt actttcctca
    74341 gtcaattgtt ttacatcata aaaggagaaa ttccacttat ttttttcgct gtttcatgac
    74401 ataaacaact acaccgatta acaaaattcc tccagctaat gctagcaacg ggttattaaa
    74461 ttctcccgtt tttgggagtc cttgagtgcc atttttttca ctttcttttg ctgtcctatc
    74521 cggatgagaa ggttttccat ttggttttcc atttggtgat tcatttcctg gattttctct
    74581 ttctggtttg tcatttcccg ggggcacatc ggcagtttct cgcacagtga tcgctttttg
    74641 ctgggtaaca gattctgtat gagcagacca tttttcccca tcaaatattt ccgaacgata
    74701 agtgacttct agcgtatatt ctccttttga aggtgctgaa aaaatagcgg tatgattaga
    74761 aaattgagca cttgactgtg tttcaacgat ttggatcttt tctgggaaat aacgcatatc
    74821 ttcaaagaca ggcgttcctt gatttggtgt gaaagttgac acaaaaactg taaaaggctt
    74881 cagttcaatg atatttcctt ctgcattctc cgccacacca tttgcttctt gtataataat
    74941 cggattattt tgttcatttg gttttgtctg atttggttct tgtacgattg catactctgg
    75001 atattccgaa gacggtgagg tttctccgtc aaaaatagca cctacagata atggaagacc
    75061 atccactctt tccttttctg tttgacgatt ccccggttta atctgcatca gtccatgaac
    75121 atggatactt ccattttcat agcgcgtaac aggctgctga ccttctttcg aacatgtatt
    75181 tagagactcg atccaatctt ttgatatggt atgtccaaga gtattttctg attgtttctc
    75241 tcgttcattc caatagtagt tcaacgagtg gtagatttga tcttcgtttt gattcaatgg
    75301 gcggatctgc tctctcatct caagattctc tgttcggaaa gataagatac cagaagcatg
    75361 gtagtctgtg tttcctgctg aagaggtata aagagccaca ttatatgaac cgttattaaa
    75421 acttgtactg ctattgacga taatatctgt tccgctattg gaatcgattc ctttcgcaag
    75481 attttcgaaa gacaaactat tgatcaattc atgagggccc gttaaagaag aaccgcccat
    75541 tttgaatcca ttaccgttac cttctccctc aattcctggt acccagccgt ttcgataagc
    75601 aactgagttt ttgatgatta cttttccgat cgaacctgtt tcatttttag caaaaaggtc
    75661 ccaaccgtca tctgcattat gatacgcgat acaaccgtca aatacatttc cttctcctat
    75721 tgtcagtttg gccgcaaatc catcggcatc ttcaaatcct ggatcggcat tttcaaaaga
    75781 tgtacagttc ttgactaagt tatgcgcggg ccatgcttcg aaaggatcat ttgagctacc
    75841 gctgatttgg attcctgtat ttccattccg ataagcatga atctcttcga tcaaattatg
    75901 atttccagaa atctgtaatc ctttttgcat agctgcacta tttgtaatgt caaaccctcg
    75961 taaaccccag tagtgactga acagtgtcat cccatttcca gttcctttaa aatcaagtac
    76021 aggacgcttg ttttctggat ttttctctgc aatcaactga atcgggtttt ctcttgtccc
    76081 gttaacgcct tttggaatcg tcaatcctct ttcgaaggaa taagttccag gcgccatgac
    76141 gattgtctgc ccagcatatg cgtatctcaa tgcttgtgtc agagataatg gattttgtct
    76201 gcttccgttt cctgcatctg acccttctgg tgttacataa actgtcgaat atttaggttt
    76261 acggtagtcc actgtatgaa gaatctcttt tgtttcataa ctagtcatct ctacgtattc
    76321 ttctggcgga tagtttttat ctggcgtaaa actgaaagta aattcgttcg ttccttccat
    76381 cagttttgtc tgataatcaa attcccgatt cgcagtgata gccaagtttt ttgcgttctt
    76441 aagcacctct cctgttgctt tatttttaat tgtcaataat ccgtcagcat ttgctctaaa
    76501 ggacatagta tgatcttggt ttcctgaatg attactagag gtcacagtgt aaatcggatc
    76561 aatcaagcga gtaggtcttt cttgcccagg aggatctgtc gcaggatctg agtaagcaac
    76621 ttcgattcct tctaccttga tcgtcatatt ccttgctgca gcaaatcctg cataaataac
    76681 cgattcatct gaagcgtata atttttccca gtcatacatg atgtcagaag ccatttcttc
    76741 tcctgtttca ggatcgtaat aatatgcctc taaaccagta ttcgtttttt tcatctctac
    76801 gtccagttta gtaatcgagt tcgtggccgg aaacagattc ccatgaccgt ttccgaatat
    76861 attataagaa cctgtttctt tttccaagaa ccctgctgaa gtctctaaag gtgttgtttc
    76921 cactgagaca ctcccaggag ctggcgcagc ttgcggatct tcagatgata ttcctgtgat
    76981 tgcccgtgtc cccaatccaa gtcgcatcgt atatttgctg ccgctcgtgt tgaccacttc
    77041 ttgtctatcc gagtcccaga catattcgat ccgactgcct agtatggcaa atgaatttga
    77101 ataaaatgcc tgcccaaaca aatgattttt tggtactgcg tctcttacca ttaaagcaaa
    77161 tccttcttgg gcattcgtat aggtccaaga ttctatttcg atcgtcgccc gcatcctaaa
    77221 gttgatgtct ttggggattg cttgataata ataggtcatt ccatctgatc cgcttgactg
    77281 gaattttccg ccgttattcg tggaagagag ccgcacgccg tcttctaatg gttcaatggc
    77341 atacctacta gcacttgtgc tttctccgaa aacaacccct ttccactctt catttgcttc
    77401 ttcagattgc ttttttgtct tttcattttc tgattctttc atcataggat catttccttc
    77461 ttccgtatcc atatcttcac tagttcttgg tatttcctcc attgttgaca tcccttcaat
    77521 gttcacgtgt gcattttctt cgttcacaga ctgttgttcg ttagcagcaa cttcaacgac
    77581 agaaattaaa accggggtac tgagcataag gagattcatc attagcgcac tgtattttat
    77641 acgttttttt aacaagtgta ttcctcccgt aacaaattag aaagcgttta cttaaagtat
    77701 tacctaattt atttttactg tcaataaaaa acaaaattat aataagtgta ttttataaaa
    77761 agaaaaaata taacttaagt tgttgataat aaagagattt attaacgtaa aaagaataag
    77821 tttttttaaa ataacgtata aaaaagagca taaaacaaag tcgtttgttt tatgctcttc
    77881 ttttattatt ttgtgctttt tattttatca aatcactttg tttttttata aaatttctgc
    77941 gcctagtgtg gactcaaaat gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc
    78001 cacggcatct ttgaaaatat ggagtttgta tcctagatta tacgcatcga ctgctgtatg
    78061 caacacacag atatctgtac atacgccggt caaataaata tctgtgatct ggcgttctcg
    78121 caaacggatg tctagatctg ttccactaaa agcggaataa tgccgtttgt ctatccaata
    78181 aacattttcc tcttgctcat agtcttggta taaacttttt aaagagccgt acaattcccg
    78241 tccacccgtt cctatcacat tatgcggagg aaataagcgg ttctccggat gaaaggaatc
    78301 ttttggatca tgcgcatcaa tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt
    78361 tgtatgagca gtcagctttt tttcaattac ttgtcctgct gtcccagtag tcagctttcc
    78421 atcctctgca acaaaatcat atgtgtagtc aatcgagatc aatgcgttca tcttagtcct
    78481 ccttatacga gatgtttgac aaattcgatc accatttctg ctgatcgttt gcctgcgtct
    78541 tcgataaatt cgtcaaagct ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt
    78601 acgatcaaaa atgggatatt gaactgacga gctgtttgtc caacagcggc tccttccatt
    78661 tcgcaagcta atgcttcagg gaaattcgtt aagatttctt tgattttatc tggcgagtcg
    78721 acaaatgtat cacctgtaac gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct
    78781 gctttaacca tttcactgcg caaatatgtg cttgcttcat aatatagagg cattcctggt
    78841 aattgtcctg ggttgtagcc aaatcctgtt gcatctgcat caaaataagc tactttgtct
    78901 gatataacga tgtcacctac ttgtaagcct tcaccgattc cgccagctga acctgtattg
    78961 atcaccatgt tcacgccgta ttgttggatc aacagacttg tggtaataga tgctaagact
    79021 tttcctatac cggaacgtac gacgatcact tcgtggttac ctaatgaacc tgaaataaat
    79081 aaagcgcctg cgcgttccca tgataatggt tcgcttaggt tctctctcaa aatttttact
    79141 tcttcttcca ttgcgccgat aataccgatt ttcatctttt taattccttc tttctatata
    79201 aatgcgattg ctaaaaaaac cacacataac attaggataa caataataag agatttcatc
    79261 aaaaaagagt tccatttggt cgtcttttcc cgttcgctga tccttgtttt cgtgattggt
    79321 ttattctttt tgctttcttt acgataaaag ctggcaatct ttttttcttc ttgctgataa
    79381 gctgtttctg cttttcgctg tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg
    79441 cgaagttcgc ttcgcgtaat aagcggtcct ttggccataa gccgatccct ctactttcct
    79501 ttggtcaata gttcccagta ttgaatcgca taaatcgtct ttgcatcaca aatcaactga
    79561 tctttcatcg cttgttgcgc ctcttcaaga gttagatgat acaactccag tacttcgtct
    79621 tcatcttgtg ctaaaggatt ctcgaccttc tctacccctt gtgcatgata aatatgaagg
    79681 acttcatttg caaaacctgg tgaaagatac atcgaagtaa gatggctcag cgacttcgca
    79741 cgatagccag tttcttcttc taattctcta gctgctgtca tttcgggatt ttgtccttct
    79801 cctggatcga tttttccagc tggaatctcc aaaatcactt tttctaaagg ttttcgaaat
    79861 tgtttgacaa gaagcagacg atcttgttcg tcaaaagcaa tgatcccgac accgccagga
    79921 tgaaatacca gttctctctt cgaagttcct ccatctggca aacgaacttc gtctacagca
    79981 acatcaataa tttttccgct atagatttct ttgcgactga ttgttttttc ttcaaattgc
    80041 tcattgttca gcatatcttc tcctgctttc tttgtaatca taactgcttt tttttcattt
    80101 ttcaattttt ctcaaaaact ccttattagt ttaccataga taaaccttag attttctcta
    80161 aatatagaaa atccttcttt agtccgatac tctttatagt agtccgtatg ctaaaataag
    80221 tatgtaatcg aataacgaaa agaattcaat actcaacgat aaatgacatg gagggtttat
    80281 tatgataaaa cgaaattggc cgtggcttct agcattggtc ttgctatttc tctttgtccg
    80341 aaacaatagt ttttcttttg ttctaggact tttattgata ggtgtagttt tagttttgtg
    80401 gggactcttt ggttcgcgca ggaaaaaggg aaaacaagaa gaaattcctg cactgactga
    80461 cgaacttgaa actcactatg aaaaaagcgg tatgacagcg aatgaaattt cattttttcg
    80521 acaaacaatg aatcaaacaa aaaatgagat caatcaattg caaatgaata tgcagcagac
    80581 agccaaactt aaagcaatcg atctccgcca tgatccagtc aaagcagcga aagctttatt
    80641 caaagaattg gtgaaagaac caaaccgact gcatgaagca agtcagtttc tttatacgca
    80701 tctgccgaat ctagttgatt tgacaaataa gtataatgag atcaatgacc atgagatcaa
    80761 aaacaaacag acctatgaaa aactagaaga aagcgcgcag atcatcgacc agctgtcagc
    80821 tttaatcgca caagattatc aaaaattcgt tgctgaggat ctagatgata tcgacgtaga
    80881 gatatctgtt gcgaaacaga gcttaaagcg tgataataaa tatgaaccag aacaaaagga
    80941 ttaactcttt tgttcataaa agaagactcg gaacattcag atgatgtctg gctgtttcga
    81001 gtactttttc taaagtagag acattcatgt aagaatcgag aacagattca acaaggagga
    81061 attttttatg gatcataaac cgactgaaac aaaagatgtt acaccagtta acgatacact
    81121 tgaagattta ttgaacaacc cgttttcaac tccaacagat acgctgacca cttcacaaca
    81181 agcggagatt gatcagctcc aagagcaaca gacagctgct cgtttaattg acaaactacc
    81241 ggcggaacgc caagaacaag ctaaacagct agcagcaaaa atcgatgcta atgatgcaca
    81301 gtctgtcatc agctacggat cggctgccca agcaaaatta agtgaattct cacaatcgat
    81361 gttgaatcat gtgcaagcac aagatattgg tcctgtagga gattccttaa ctgaactgat
    81421 gtatcgtctc caagaagcca atcctgacga actgcgtgcc ggagaaggga acatcttcca
    81481 gcgtgtcttc gggaaggtca aacaatcgat ctatgaagtg acagcaaaat atcaaaagat
    81541 tggtgcacaa attgacaaga tttcagtgaa attagataaa gaaaaagacg gtctgctaaa
    81601 agataatctg atgttagaac agctttacca gaaaaacaaa gactactttg atgcactgaa
    81661 tatttatatc gctgctggag aattgaaaat ggaagaactt caaacaacga tcattccaga
    81721 agcgatgaaa agagcagaag aaactggcga tcagatggat gttcagattg ccaatgatta
    81781 cacgcaattc ctagatcgtc tggataaacg tacgcatgat cttcgcttag ctagacagat
    81841 cacgatccag caagcaccac aaatccgttt gatccaaaac acaaatcagg ccttggctga
    81901 aaagatccaa gcatctatcg ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc
    81961 attgaccttg cttcgtcaaa aagatgcagt aactgctcaa cgccaagtct ctgaaacaac
    82021 aaacgactta ttgaagaaaa attcagaaat gctgaaaatc tcagccatcg aaacagcgaa
    82081 agaaaatgaa cgtggaatcg tggatatcga gacccttcaa accactcaaa atgatttgat
    82141 cgaaacaatc caagagacat tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc
    82201 agaagtagag cttggacaca tggaagaaga tctgaaacaa aaactgttgg atttaaccca
    82261 atagatcacc tttcgcttgt tactaaaaca aacgcccaac aacagtttat tacaactgtt
    82321 gatgggcgtt tttcttatct ttcttctaac ttctccattg gagtctattt ctcattcatc
    82381 ttgctgtgtc catagccata gccaaaataa atgatcagtc caataaccat tacgataccg
    82441 aacacgatcc atgtcacagt ttgcagacga agcatcaata caatacaagt caagatggag
    82501 acgattggca atagcggaac gaatggcact ttgaattctc cagcttttgg tttgccaaac
    82561 atttttctca acttcagtaa gcctagacta acaagcatca aataggcgag cgccatgata
    82621 ttcgttagtt ccgctaatac attcaatggg aaaaatccgg caaagaatac agctgccaca
    82681 ccagcggcat aagtcgcttt tttcggtgta cgatgctttt gatcgatttc tgtcataaac
    82741 ttaggaagta agccatcctt gctgacagca aacagtaaac gagcaagtga atacatcatc
    82801 gagatcgtta ccgtcagtag cgttaaaatt gcccctacag cgatcaccgc gcccactgtt
    82861 ccatgtccta catacctcat tgcaaaagcc actgggtcat tgactcctag ttcagtatac
    82921 ggaacgattc ctgtcaagat cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc
    82981 gaaccaatga ttcctcttgg cacatctttt tgcgggtttt tgacttcctc ggctgccatt
    83041 gcaacggcat caaatcccaa gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg
    83101 gaaaaaccaa aaggcatgaa tggctgccag ttatcaggtt taacgaaaaa tattcctaca
    83161 atgataaaca acgcgatgat accaaatttg acatatacca tggcattatt caaacgtaat
    83221 gctttcttgg cttcaagcga cacgatgtaa gtgacgaata tcactacgag caccgcaatc
    83281 aagtcaatgt atgtaccatt ttccggattg tacccgccag ttaatgcttg tggaagttcg
    83341 gcccctaaac ttttcaaaaa accttgcata tagccagacc atccggaagc aacagaggaa
    83401 accgctagta aaaattcccc gatcaacaac caccctgtca gccaggcagc aaattcacca
    83461 aatacaacat acagataggc atatggccca ccaatcactg gcactcgcga agcaaattct
    83521 gcgaaactta gtccagacaa aatgacaacg attgcagcta agacaaatga aagtgacagt
    83581 gctggaccag cattttgatt ggccgcaacc cctgtcacca caaatattcc cgtaccgatg
    83641 atcgcaccaa ttccaagcat gatcaaatca gacgttttta gttctttttt catctcactc
    83701 gtatgattga gattgatctc tttcttacga aacaaatcca ttgaagttcc tccccatttt
    83761 aggttggaaa acagcgatta aactgttttc atatacccta aaaggatacc acctaaaatg
    83821 actaaaaggc aaccaacaat aacataaatg agctcttttt tcgtttttct ttcgccaagg
    83881 atgaaaatcc cacctaaagt agagataatg atccccattt gtgaaagtga gaagccaaca
    83941 gctaaaccaa gtgatttgac tgttagaagc atgcagatgt ttcctaagcc ccataataat
    84001 ccacagatca tgtttttcca cacaaactga tcgactttaa ctttacggaa agcaaagaag
    84061 cttgcgccta agatcattcc gatactttga ggtaagataa ttgacatcgc atccaaattc
    84121 gcccagttga cgatgatggt atatacacca taaccaatcg tagagaagat caatgctcta
    84181 aaacccttac caaaatcaag cattttatta tctgtatcta tttttccttc gtcatcttgt
    84241 cgggcagtca aatacgcacc gcttacaaga agagctaaag caataatacc gtatacataa
    84301 tctttcgttt gtgtccattc atggaagaaa acggctcccg caatcgtatt caaaattaat
    84361 tgaaaaccag tagacaatgg tagccctaca gaaacaccca tgtacttcat tccatggaac
    84421 tgtccatttt gtccaacact ccaagctaag ccggataaaa atccgatcaa gaaaatccaa
    84481 ggggtgatta caggactaac aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc
    84541 gtcattccta gtgtttgttg attggcatcg ccaccaattt taccacttac taaaccaata
    84601 ctaccccatg ccaacatagg gactaatgct attaaaattt ccataaccaa tctcctttta
    84661 tttcacatga taagaaactt atcagcgaaa acgttaacaa cacaagcact ttttttcgtt
    84721 tctaacgatt ttctaacgtt tttcattata aaaatttaag attgggaaaa ataaagtcat
    84781 tgttgtccct tttccttcag ttgattcagc cgtcaaatgg atacctaatt tttcggccag
    84841 attttttgct aaataaagtc ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct
    84901 cccagtaaat cccttatcaa agatccgttt caagtcttcc gctgggatac cgatccctga
    84961 atctttaact gaaaggctga ctccttgcgc ttccttttct atgctcacaa tgatgtttcc
    85021 gtgatccggc gtatatttga tggcattact aagcaattgg ttgaagataa aagcgatcca
    85081 tttagcatcg gtcaaaacag tatgatcctc accaacgatc tcgtagcgaa tatttttttg
    85141 gataaaataa tttgcctgac tgcgtatcac aggctgaatg attcctttta gtccatattc
    85201 ttggatgagg tagtcccgcg agaaactgtc gagtcttgca taatacaaaa cttgttctac
    85261 gtattcatcg atttttgata attcattttc tagtaatgta tacttttgat cgtcaatatc
    85321 gaactcgatc gaacgaacta gcaatttaga agcggaaagt ggcactttga tttcatggac
    85381 ccatgagtca atataatctt tttggtcttg ttgattactg attgctgatt gcattacttc
    85441 ttggtgttca cggatcagct gattgatata ttcttctacc agtttttctt cttctgtcct
    85501 cccaccactc aaataatttt gcaaagggga agttttttgt atgtcggcta attttcccca
    85561 ccatcttctc ttgctcaagt aaccacccag cagataaaaa atcagaaaga cgccctcaat
    85621 caaagctaaa taagcaattg tgctcaaatc aactttgatg tttggcgcca gccacatcac
    85681 aaagatcgtc aatgcaataa aaaacagcca tcccaagatc aatgtccact gatctttcaa
    85741 atatttaaaa aaattcatct taaccgctca tcctttcagg gaacgatata tccctgtcct
    85801 acttttgttt cgatatatcc ttggatacct gcctgttcga tttttcggcg taaccgattg
    85861 atgttgactg tcaatgtatt gtcatctaca aatcgttcgt catcccacag tgctcgcagc
    85921 aatttttccc gacttaagat ctttccattg tgcttcatca aaataaataa tagacgatat
    85981 tcatttttgc tcaaatcaat gatctctccg ttgatttcca tacttccgtt gtcaacattc
    86041 aacgtaatgc cattgtggct catcatttca ctagataatt ctgtgtagtt gtaagaacgg
    86101 cgaagtaatg cattgatttt tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa
    86161 tcatctgccc ccatgttcat agccatgatc atatccatat tcgtattcct gctggagata
    86221 aaaatgatcg gtactttcga tacttcacgt attttttgac accaataata gccatccaaa
    86281 acgggaagat taatatctag taatacaagc tgtggttctt ctctttcaaa gtctgagaac
    86341 acttgattga aatcggtcgt tccaaaggtt tcaaattgcc atttctcaag ttcctccatg
    86401 atcaattgtc gaatgatctc ttcgtcttcg actaccatga tttttgccat aaaaaatccc
    86461 tccatctttc ttcttgctac tacaatatca aagaagccct tgtgaaacaa gttggcttac
    86521 ctggaaaatg tcattttagc aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt
    86581 ttgtctcttc caagcagctt aaatttatta tgttattctt aataattggt ctattatctg
    86641 ttatttttct ttcgttgttt ttgtctttta aattttcgat aacaccaata accgaaaagc
    86701 gttagcacta gcaatatacc tagaaggagt ataagtaatc gataaagatg gtattttttt
    86761 gtctgttcta tctttgaaga gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt
    86821 aatcggtgag tgttcacggt gtaaggtgta caagtgataa gtgtgactag atcctgcttt
    86881 tctctaattg ctaatgtatt tgtttttgtt ggaaggacaa caattttctc aacgacttca
    86941 taagcgagca tccgaccata cacttcaaga tagaatcggt catcttgctc catttcttca
    87001 agatccgtaa agagtttttt ctcagcaata ccgctatggg cggtaatcac ggaatgtgta
    87061 ctctctcctc cagttggaaa agaggtgcct tgcaggactg tcgctcctct atctaacaaa
    87121 agatcattcg tttcatcaaa taaaggcaag ctaacattga ttttcggaat atagatggct
    87181 ccgatcatat gttgttcgta atattctttt cctggatttt ggacatttct aaccgcttgc
    87241 tcaaaaggat ctttgacttg tcccattcct gggattactg atgtatgagc aagcgtctga
    87301 tttttctttt catgcactgc taattgttgc tcatgttttt cttctttatc gcgttcaagc
    87361 tgcttttgat aattctcgat ccttctttga tccaaataat tgctgagagc atctgctaaa
    87421 aatggatatg taaaaactgc agctccggca agaaacatca ctgccataaa taattgtaat
    87481 aaaagactga tttgggtttg aggtctatta cctattttct ttttcatttt cttcaccttc
    87541 ttatttaaaa aaactgcagc aacctttata aaaccgattg ctgcagctta tttttgttgc
    87601 ttatacttca gacttcattt tagatttacg gtaccagctg tacgctccaa tcatcaaact
    87661 tattccgatt agtaagaatg ccaagagccc gttcccacca gtagcaggaa gtgacccgcg
    87721 tttcatattc ggtactcttt ctggccctgg attttctact cctacgatct gaatttcttc
    87781 attatagctt ccataagaga tgataaattc agtaaaggct gcctcacctg ttggaagtac
    87841 ataaccttct ggcgccatgg tctctttcat ttgataggtt ccataatcta acccttgaac
    87901 ctcaaagtat ccatttccat ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc
    87961 aattctatct gctcctgatt cataaacagc taaaggcgac tcccctttat aaaatattgc
    88021 gtattcagaa cggtcttcat tccacaattc gaatcttgca tctttaagca attcactgct
    88081 tgatgcatca tatttttcaa actttcttcc acctgttgtg actttctctg gcggctctac
    88141 tccttcttca taatcaaatt catcatttcg tccgatttca ataaccgctg tgttgttatg
    88201 gaaatcatcc gcttctagtt ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc
    88261 tccacgtgca tctgctaatc tacctaaagt atctactttg gctactccat tttcttctgt
    88321 caaaatcgtc acgacaaacc cgttgcttac tacttctact atataatctt gtccttttgt
    88381 taaaatcgtc tcgccggctc gcacttctat ttcctgatca taataagtta gaccaactgt
    88441 aggcgtatct gtgattttgt aatttttgat aacagcgctt ccgttttcaa ggacagaatc
    88501 gatgaaataa ggaataggtg ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc
    88561 aatataagaa atcgtttctt gttctccagc ttcattaata atcgttactt gtctaaggtc
    88621 gctttcctct acattcatct gtttatctgc ttctctgatt tcgtttttcg gatacaaatg
    88681 cacatctgta ttgtatgtgt ctttccatgt agaaccatcc cacacgccat ccaccatatc
    88741 aggcatcatc actggcatca taacaaccat tggagacgcg attctggaaa tatgagctgg
    88801 cgaataagtt tctaagaaaa gatagacttt atctctagaa gaaggtgctt cttccgtcac
    88861 ttggacggta gagaataccg cttctccagc ttcattcgtt gtttgtttgt caatacttgt
    88921 cagtcgatat tttaaaaacc aactttctgt gtgccgtttt tgaatccatt caatcgcttc
    88981 ggcagaatta agaccgtctt ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta
    89041 atatacttcc gttacgtcaa acaaatcaaa atcgactcct ggaatatttt cttcaccaaa
    89101 atccgtagaa accaatccgc tattcggtcg ttcttccgga acagatgaga aacctttttt
    89161 gtgtaatgta atcgtcactt cacttggttt ttgtgcgact tcttcggctt tgactagttt
    89221 tccgttcccg aaaatcccta ttcccgcagt aacgagtaac agtacactca ttcccgtgac
    89281 aatcattttt ttattcattt ttttctcccc gttctttttt tattaataaa acagcaccta
    89341 acaataaaag aattcctagt aaagaaatca acgttcttgc ttcattcgtt tgcgggaata
    89401 cggaccattt tcctgattga cctggattgc ttggaccact cggctctttc ggtttgtccg
    89461 ttgtttcttt tacggtctcg ttataaacac gtggtgttgc ggattcatag gcagagactg
    89521 gaactttccc ggattctaat tcagccatct cctgtccgtt aactactaca tattgtggtt
    89581 ggccttttgc atccacccaa aattcaggga tgatgacctc gattctctta cttgcttcat
    89641 ccatctcgta accttccaca ccttttaatt cttcaaaata ataggtgcca gaaggcaaga
    89701 aacgttgtcc tgtcgtaaca aggccctctt tatccgaaat gaatttgctt acatttttgt
    89761 cattcaaagg atcattgtct gctggtttga tccattgatt tttcaagtca ttagcaggag
    89821 ccatatctaa ataatatttc cccccttcta tcatctgata tagagcaaaa acagcacctt
    89881 ctaatggttt tcctttttca gttgttcctc tttgacgtcc atacttgaag aagtatggat
    89941 ctcttagata accgacattt ttaggataga tatgaatctc ttgcagttcc tcggttgatt
    90001 ctgtcgggtg ataaattggc aaaatagcag caattgggat agctgtctga tcgatatcaa
    90061 catccaatcc aacttctgga tcaatcgatt cttcgaaaat caaataaact gcatcccgat
    90121 cttggttttt cttaggtgct ttgatacgtg caatcccgtc tcctaatccg ccaaccgtat
    90181 ctttcccagt tgttaccgaa gtccctattt ggacaagcct ttcttctgtg attagctgac
    90241 ggatattgtt tctgttcgtt tcagcgattt gctggacgaa ttctttagga gtcatgtttt
    90301 cagcagcttt tttttcatga taataatcgg tcatgtcgta aatggcaaat gtcgctccat
    90361 ttagtggcac cgtctgtgaa atgatatctg ctgtttcccc tgcttcctca gagatagcca
    90421 agccatcatt ttcatggtat tcaaattgat cgttataatc gatatctcga atcatccgtt
    90481 tatgcaacac gaattccacg acatcttccc ctgattcttc tgccgacatc gcatgtggaa
    90541 gaaggactgc tgcaagcggt agtaaaacaa gtaattgaag catcaaacac aacagctttt
    90601 tcattacctt gctccctcct ttttagattg tctgacaaac aaaccatagg ctgctcctgc
    90661 aagcgccatg aagaatgcag caagcaggat atagaataat cgtccggatc ctcctgtagc
    90721 aggcaacgga ttttcaggat cgttcccgac actaagctgt aatctgattc gatttgtctc
    90781 caaaagttca tattctaact cttgctgtag atcaggcgtc tctgcttctt cgtttggata
    90841 atacgaaatc tcaactttta gtgtacccgc ttcttttcct ggaactaccc gatcattttc
    90901 tatgtcaggt tcttctcgat aactaatttc taaaataaag taacctggaa gttttctata
    90961 gctgtcaggt gacttcactt ccttgatccg atagacccct tccaacaagt ctgaagcatt
    91021 gaatagggaa gaatcgtttg gatctggtgc caagcctccc tcgatgattt catactctcc
    91081 tgcactattt tgtttctcaa ttgaaaattc tgcgccttca agacgtcggc tattcatact
    91141 gtcaattttc aaaagttcca aatcgattgg cgctagttca tcttctaagg ttacttgtag
    91201 tacaccatcg atgatttcat agccttccgg caagggagca gtttcgtctt cgatgaccgt
    91261 atcttcaatg ataaacttac cttcttcatt cagtgcaaac tggaacactt gatcagatag
    91321 ccgataccca tttggtgcga cagattccga gacctcatat tcaccaatag acagatgcag
    91381 aggttggtcg taatttaaat cgacatactt ttctactcct acggcacgag aatagaagta
    91441 tccgatccca ggcaaacgtt tcgattcttc tggttctaac ggattaccag tgtatgctcg
    91501 atacagcggg aaggaagaat cccccacttt cctgatatcg aaacctgcac catctaatgt
    91561 atggccttca tggtctaatt ttctaactcg gatctcccga taatcaaacg tattgtaagc
    91621 agtgaatgct acccataaac gatcctcttc atctgttgta tacgtgagat attgccaatt
    91681 ttcttcacct gtttgcttga gttggatagt ccctatagca ttatcgtaaa gtcgcaagat
    91741 ccatgtgtca ttttcatcag gttctttgaa accttctggg ttctggctct caatgatttt
    91801 gtattctgca tagttggtag tttcttcact atccttataa acaaatgaat ctggctctcc
    91861 gattggatga ataactttct caccatccac gattacatat ttcccgtctt catctactga
    91921 atagaacgtg agtctccctt tctcttcctc tacttcgttc acaccagaaa gccctttcgc
    91981 tactggatat ccgatttcca gtccgttacc atctgtttca taaagttcaa actctgcgcc
    92041 attgattggc atattactgc cttcaattag tttattgata ttcatgtgga ttggcttgaa
    92101 ttcatttcgc atttccagtt tgacatacaa ctcatcatac tctgtgcctt ctatgttagg
    92161 ctcttgtaca acagaaagct tgtgatattc tccttcataa taggaattcg gatctttacc
    92221 ttccggtccg ccgactctcg aaccagatgt atattcatct acaagctcgt agtgacctaa
    92281 catctcatct tcgttggctg catatttttt gatttggaat tgatacgtct catctactaa
    92341 ctgataacgt ggtggtccag cgatctctct gatccaaaat atgtcgctga ctgctccagt
    92401 ttcatctcct aaaatcggca tcttcgtttc aaagatcgct attccgtccg tggtagtttt
    92461 acttgtttcg attggttcgg cttccccaca ctctggattt tggtcatata atgcaaattc
    92521 gactccagcc aacggctcgc cattttgacc cttcttgctg acttggaaat tacggccttt
    92581 atgttcgtaa cgaatggaag aagatgggaa atacatccgt ccagtagtga tatcattctc
    92641 ataatcctcc aggttctgaa tataggccat attgttcact gtgcaaaaat ttccgtcttg
    92701 agctcccgca tcaagtttta cttggtactt gatcctgacc atttcccctt cacctaaaga
    92761 aatcggagaa aatttataag cttggttttc ccgtccgaca tacgttgtcg gagtcgccac
    92821 tacttctggt ggggcttgtc catgacgttc ttcatcccat atttctggct cttggtactt
    92881 catgccgttt tctcctggat ctagatggta aaactgaata tctgccgatc tgccagctcc
    92941 aaatggtgag acattaaaac cagtttcatc ataaagtgtg gcttgctgga tcgttctctt
    93001 gaaggtagaa gagatatcct ccaaggctgt aacaatgtca ttcttgttgt tcgcattgta
    93061 atatcgcggt gttacgccat cttcttctaa atcagcaata tttttcaata catttcgtcc
    93121 aatagcatct agtcgttgtc tcccgctaac gtttccatcg attcctaaac caatggagaa
    93181 gatacttggc gggttctctt gatacttgtt tttcagccaa tgatgataag caactgtgtt
    93241 gatcgcagat gacttttctt gcgaagtagg actgccattc gtattgttat tagaagaacc
    93301 atcaccaacc cagtgagtcg ctttattatc tccttctttt acctctccat aacgatagtg
    93361 tacatttact tcatcttctg gacggttaaa gtcagatgca taggtaactg gatgagtaaa
    93421 ccctgcatta gaagtattcc cagaaaactc ctcaaaattt ctgaacaaaa cgtcattatc
    93481 ttcagcaatc ttatcttcca tcacagtcca gttgctccaa gctccattag tgctcgtacg
    93541 ataacttgac tgaatcggtg cataactgaa ggtcggtgtc ccatctccca caacgattag
    93601 gattttttct ggatttctat tttctccgcc attgtcttta tacaatgttt catagccatt
    93661 tttaagccct agtgtaagcg gagttcctcc gattggtgta cgatttaatg ctgtattctc
    93721 acttcccgtc aaacgattga tatcatttgt caatggaaag atgtccgaac tgatttgctc
    93781 atggttattg atagctgtac tattatggta aaaattcacc ataccgattc gaatatccag
    93841 actcgggtca gagagcagat ctttactcat ttcgtttact gcctcaatca acgcagcatt
    93901 tttagtttga ctattggtcc cagcggttaa ttcactcata ctcgctgact tatctagaac
    93961 aaaaacgata tcgattggac tgatttcctt tttctcgccc ccgatgacat ctagatagac
    94021 attgtaaagg gttgttgcat cttcaggatg tagatcagat gctgtgctgc ttggcagaac
    94081 aggttctacc cattttttga tgtacgcccc gttataatcc aagtaactgt tttcaaaatt
    94141 ccgttcattt ccccaaagct gaacggtttg agctggaacg tttctatctt cttcgcctgc
    94201 agttgatcca ttattagtag catagtcgta atttcgccag ttcgctcccc cgtttgtata
    94261 atcactatca tcgatcaagt tctctccgtc atatggcgag cctttgtctc cttgatagcg
    94321 ttcattgctt tgtgtccaat ttcctaacac ttttgtatag tcattcgttg taaacttcat
    94381 ctattctttc atcaaccacc acttgaagag gtagttaccc ttcgttcaca tcggggatct
    94441 tttctgtttc aaaaatcaat gtgccggttt ctgcttcttt tgaataaact tttccgacat
    94501 accattcttg accatcgatc gtctcgactt ttaggtcttc tccatcttca tcataggagg
    94561 atagacctgt tccacgaacg ttttttacgg taccaatccc ggcggctgcc gcatcgattg
    94621 ctaaacgtat tctaccttca ttggcacttt cctttttttc aaaagccaga tgccattcaa
    94681 gggtttcttc tttttcaata tacttccctt caatttttgc atagtcctca tctaagaata
    94741 cctcatcctc ctttgtttct gttgactcag cagctacatc cagggtcgtc atatgataga
    94801 ctgactggaa atatggtcca aatagcaaac tgacagttac taactgcaaa agcttcaatt
    94861 ttaacccttt cacatcctta tactcctttc aaaaatattt gtcttctttc acaacaatca
    94921 acaaaacaat gcccatctaa ttataattta atttaatttt aattttaaat caaatgatat
    94981 cctttttagt taaaaaacac caattataca acatttatta ttaaaattaa attataaaat
    95041 tttataacgt tctcatttaa caaaacttaa gttattaaga caaatgaata aacaactacg
    95101 ctttcctcga aacaaaaaaa taaagcacaa atgcgcattt acgcactcat gctttattcg
    95161 tacaattaat tcagataacg gcagcgcata ggatgccaat aacacttcct aaaatagctc
    95221 ctcctaaaat gtcagtagga taatgtaaaa gtaaaaaaat cctcgaataa gcgatcaaac
    95281 aagcaaataa aaaggctaat attcctgcaa ctggacttaa ttcgaacaac gcagctactg
    95341 ataaaaaggc tagcgctgta tgtcctgaag gaaaagaaga agtcttcggt ttacttgtgt
    95401 aaaggacaag ccgacgattt ttttcataag gtcttttccg tcttgtcgtt tttttgataa
    95461 aaagcaaacc aacaaaaaaa tagatcaaac tgccgataag aatggctaga ccatattctc
    95521 tagtatcagg tatcaagact aaaacagcag caaggagcaa ccaaataaaa ccaccattag
    95581 agatattact gataaatttc aaaaaattga tttttcgact atccgttttc cgttgcttgg
    95641 cgaacttgtt gataatacta tcatcgattt tagcaatcaa acgcaacatg ttattcctcc
    95701 tttttaagaa ccttttcttg cgtacgatgg ataaaaagca ggacttgaaa ataaaacaag
    95761 aaactgaaac ctaataaagc aaaatagatt ggtcctgcaa accagaatgc gacaagaagg
    95821 atcaacgtgc ctgccaaata gaaaagcgcg cttttgaaat cattaaaaaa caagaaaaat
    95881 gcagtaatcc aagaagcttc tttttgttcc agttgaagca agattttgta actaaagaga
    95941 agcgcgctta atagaagcaa aaacaacgta gtatacatca atgtatagcc aattccccca
    96001 ccccaatagc ggaaaatctg ataatcgatc aatagaagaa gaaaataaac acttgctaaa
    96061 ctatcttgct ttccataaag agagaagttc tcacgaaact ttttccagaa tagtgaaccg
    96121 agctttttct cttcattttt tttgtaatga taaatcatat acagaactgt tcgacttgca
    96181 gttcctaatg tgaaaacagg caagacgcac aggaaccata gtagctgtaa atggataaga
    96241 cgtggaatcc attcaatgat ttctaagaaa taatcattgg cttttttacc aatcgatcga
    96301 ttcatcatcc ttttgaacct cctgtcaaat ccatcccttc aatgaaataa cgttgtgcaa
    96361 aaaagaacag caaaatcgtc gggatcaaga caagtgttgc tcctgccatc agataattcc
    96421 attgtgtcgt tgcttggttc tgaaaaactt gcaagccaat ctgtaacgtg tatttttctt
    96481 gatcttggat atataacaga ggacctaaga aatcattcca agctgcgttg aaggaattga
    96541 tcgcaatggt aatcaaggca ggtttcgtca atggaagcat caaatgactc caaatataca
    96601 aatgattcgc tccgtcgatt tcagcagctt cgatcaattc attattgatc gataagtaaa
    96661 attgacgcat cagaaatata ttgaacgcat tgccgaagaa cgatggaacg atcaggggca
    96721 aataagtacc tacccagccg atcttggaga ataatacata ttgagggatc atcgttacaa
    96781 accccgggat catcatcgta gaaagaacca aagcaaacat cagctttttc ccaggaaaat
    96841 caagcttcgc aaatccataa gcaataaaag aatttgaaag gacattaccg atcactacta
    96901 gcacagtgat gaataatgta tttcttgcat agcgccaaaa aggaaaggcg gcaatcgtat
    96961 ccagatagtt ttcccatacg attttttctg ggaaaaaggt cggaggataa cgaacgattt
    97021 cttccattgg tttcaatgaa gtaaacacca tccaaagcaa tggaatgatc aaaatgattc
    97081 ctattactgt catgaggaca taacttagta ttttttttgt ttgccgattc acactggtct
    97141 ttttgaaata atccatcatt tgccgtcacc tccagaataa tagacccact taggtgcaag
    97201 cttcaaattg acaaacgtca gaaccaacac gatcagaaac aggatcattg acatagccat
    97261 cgcataaccc atatcaaaag ctacgaacgc cttgttccac atatgcagat tataaaaaag
    97321 caaagaattt gcaggtccgc ctgttccatt tttcgtcatg acataagctt cttgaaaaat
    97381 ttggaaagaa ccgatcgtag aggtgatcac atcgtaaaaa ataataggag tgattaatgg
    97441 caaagtaata cttttgaact ttccccaaac gcctgctcca tcgatttctg ctgattcata
    97501 taaagatcta ggaacatttt gtaatgtagc taagtaaagt agcattgctc ctccagaact
    97561 ccatattttc atcaaaatca acgcaggttt cgtccattgt ggatcaaaca gccaagctgg
    97621 tccagggata tggaaccagc ccagaaatgt gttgatcaat cctgcagatg gagaaagcag
    97681 ctgcatccat aagatgtaga ctgctactcc tgataagact gccggtaaat agaaagctgt
    97741 acggaataca ctgattcctt tgattttttg attgagtact gtggctaaaa acacactgaa
    97801 aatcgtcgtc agcggtacat tgaagatgac gaaataaacg gtgttgtata acgatttcca
    97861 aaataaatca tcgtgaacga acattctttt gaaatttgct agaccaatga aattcatctt
    97921 ggatgtcaaa ttataatcgg tgaaactacc aataactgaa aaaagcatag gccctagcat
    97981 aaataccaag aacccgataa taaaaggcga gataaataaa taggcccatc ttgcttcttt
    98041 tttctttcgg atagataaac gcttcaaatt ttcttgcctc ctctgtacct agacagtcaa
    98101 aaatggatta ttgattttgc ttgactaagt tttctactgc tttttgtgca tctgccaatc
    98161 cttcttttgg tgattttgtt cctaaaagaa tctggtcaat ctgttcattc actaatgtcg
    98221 tgtaatccgg agcatctaaa ggaactggtg tgatcaccgt atttttaaag ttttcgtccg
    98281 ccatttgata gatcattttt cctttatccg ttaggttgtc attattcact aaattctcat
    98341 tggctgtctt gctcgccatg atatcaaaac ttttttcgcc gaatttttct tgtacttctg
    98401 gagaagaaag atatttgatg aattcataag aggcctcagg atcttttgct ccgtatggta
    98461 cttccaagac gaatccgcca ccccacgacc aatggcctga accttcttct ttttctggga
    98521 gctgcatcac gccaaaatca aaatcatccg gtgcattttc catcaaacta gagtaataat
    98581 tgatattttg ggcacgcatg gctactaatc cggaaataaa tggatcagat acaccagacc
    98641 caaattcggc ttctaattta ttgatcgtat ttttcccata ataatcttgc cagtcttgga
    98701 tccactccaa ggcttccact ttatttttag tatcaatctt gactttttca tctttgtcaa
    98761 accaactagt cccgttatct gcgtttagcg cccatacatc agcccctaaa ttccataatg
    98821 gataaaaacc gatgcgtgta aattcttttc cttctttttt atctagttta cgtgctgttt
    98881 cttctaattc atcccaagtt gtcggtagat cttgttcact gattccagct tctttaaata
    98941 actttttatt ataaaaaatg acttgtgtat ctgtattgaa tgggatcgca taaggatcac
    99001 cttcatattc tactgtatcc caaagctgcg gataaaaatc cttagaagtg tctttttcta
    99061 catatttgga gagattagtt gcttgctgtg cttctgctct ttgggcaacg gaatttatgt
    99121 cctgtacaat aacatcgggt gcatttccag ctgttattgc agataaagat ttagtccaga
    99181 tgtcacccca tggctgatac ttataattta catggatctc gttttgtgat tgattgaaat
    99241 cctcaatgat ttcttcgata gcttcttttc gtgcaccgga tccccaaaag gaccagaaat
    99301 caatattttt tcttccatca cttgtttttt cagaagaatt tgctgcactt gaactggcat
    99361 cgcttgcatt acttgatccg catcctccta ataaaaacaa tgctgaactt gccagcaacc
    99421 ctgtaaacaa taattttttc atattttacc tccaaatttt aatgtaaccg atgccggttt
    99481 taatataacg atatcatttt gttctgaaaa taacaattta taaggtatga ttctaaaaaa
    99541 attattaatg ataccaatac tttatataat tcgcgagcgc tttctttgaa ttttttaatc
    99601 tttcgtagca gaatgagaat gtattaaaaa tagattagga ggaattttga tgtttagaca
    99661 ccagaaagaa ttgcagtttg aagcaaaacc agatcgtccg aatccattaa tcgcgaaata
    99721 tctgcaggag ctgatcggcg gccaatatgg cgagatgagt gtagctatgc agtacctgtt
    99781 ccaaggctgg agctgccgag gagaagaaaa atataaagat atgctgatgg acattgcaac
    99841 agaagaaatc ggccacgtgg aaatgttggc gacaatggtt gctcgtttat tagagggtgc
    99901 gcctgttgaa gatcaagaag cagcgatgca aaaagatcca acaatcggtg ccattatctc
    99961 tggaatgaat ccacagcacg cgatcgtagc tggcttagga cctcgtcctg ctgacagcgt
   100021 gggcaatcca tggagcggtg cctatatcat tgcgagtggc aacttgttag cagactttag
   100081 agctaattta aatgccgaat cacaaggaag acttcaagta gcacgtattt acgaaatgat
   100141 cgatgatcgt ggcgtaaaag atatgctgtc tgttctcctt gcaagagata gctatcatca
   100201 aaatctatgg gaatgtgcag tgaaagaatt agaagaaaaa gaaggttcaa tcttagtccc
   100261 aagtactttc ccgcgtgaca aagaacgaaa agacattgct tatgacttct ataacttctc
   100321 agagggagac caaagtagtg aaggttcttg ggctagtggc aaagcactgg atggcgaagg
   100381 cgaatatcaa tggttgaaag aacctaagat cgaaggcgct gcaccaaaat tagaaccagt
   100441 aacaccaaaa atgtatggga cacctccatt caagaaataa atatctaccc gtagaaacac
   100501 ccgttttttg ttccacacgc tttgtgagca aaactgacta aagtcattaa tcataaccac
   100561 ccttcaaaag ggtggttttc tctagagcta taagctcaaa tcaccggcca gcgcctaaag
   100621 acgctggctt tcatatttta tgttcaagcc atattgcctt tgccagtttt gcccacctct
   100681 taaaaggttt atgtactact ccttagccct taaaaggatc gtcttactct cgtacactca
   100741 gcttatctaa cgcactatca tgtttttctt gttcttgaat gtactttttg atggtcgctt
   100801 cattttgtcc tacttttgtc acatagtatc cttccaccca aaagtgtcga tacccaaatt
   100861 tatattttag atttgcacaa tctcttgatg atctcctgaa tattggctcg atattgatta
   100921 taaattattt ttcttctata ctttggtgtg aatacaatgt ggtatttaca catctacttt
   100981 gtacgggcta aactatttcc ctttattgct ccacccgcat agcaagtggt tttgtatttc
   101041 aatcgctcgg cgcgctcaat taggtctaaa gacaataaat ctaaaaaccc gctgcttcac
   101101 tgacagaaac aacgggtttt cagaaaaaaa tgaaatgaaa aagatctctt acggcaatct
   101161 attttatttt tttgattttt tataaatgga aaatgcttca agaagtccaa gaaataaagc
   101221 cgtataaaaa caagaaccta tgaaatcttt atttattaga tattcaacag aaatcccgat
   101281 aacactagcc aacaatatat ttatcagaag tgtaaaataa ggatgctttt tatagaatag
   101341 aactagtgtt gttttcatag actctccttt cttcatattt ccaattaatc acaaattcac
   101401 ggcattacta gaaaaattaa aggggcgcag aagtggaaaa atactcttcg actttcccta
   101461 caaaccaggc catcttgtgt ttaataacta taccaaatct aaggtacctc taaatctaaa
   101521 ttccatttgt ggcggtgacc tccaattttt aactcttcta tctcccatcc agtatacata
   101581 accttgatac ttcgaccact tatatatcga acacacaaat acctatccat tgcaggagta
   101641 ctacctaact tagttgctac ttgcatctaa ataacagctg aaaagatagg acttactctt
   101701 agtaaagtat ctgaactatt aatttttaaa ctatcatttt tatcctcaac tacatgcaca
   101761 tctgttgcaa cttcactttc tgtctcatcc agataaatga ttaaggaatc gggaattgca
   101821 atatcttcag ctgacgctgg aatagttgag ctaaattagt catcatatct ttattataaa
   101881 ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa
   101941 tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca acatataccc
   102001 tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt
   102061 ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc
   102121 aaaataaaaa aaaacttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc
   102181 tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat
   102241 caattagaga ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga
   102301 ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg
   102361 tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg
   102421 tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg
   102481 cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa
   102541 attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt
   102601 agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc
   102661 gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa
   102721 cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt
   102781 aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct
   102841 ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta
   102901 aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat
   102961 ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc
   103021 ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt
   103081 aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag
   103141 tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat
   103201 atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca
   103261 ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat
   103321 ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat
   103381 tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt
   103441 ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat
   103501 ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg ttttgtttaa
   103561 agtatatctt attggatata aatagaaata aattgtagcc agttcatttt ttctttaact
   103621 aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa atactatagc
   103681 taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca
   103741 acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt ttgctctgac
   103801 aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac tgttcggatc
   103861 aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag acaagacttt
   103921 gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt acatgatcca
   103981 actcatatca atgtggataa atggcaacct cttatctata aattcagaga atatacaact
   104041 gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc ataatgataa
   104101 acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa tagaagctat
   104161 acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat gagcatttct
   104221 tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg ccgctctttc
   104281 tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct gctgagtaat
   104341 gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt cagtcaagac
   104401 aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag tcattatgat
   104461 tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat acactatttt
   104521 ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcattgg gaaaacacaa
   104581 cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca
   104641 ttaatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg
   104701 aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca
   104761 gctgctaatt ctgctgcctg cttcggttgt ttcgtctggg cttctcgata ataattctga
   104821 aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaatctt
   104881 tttttctatc atacactgca acaagaagtt cgcaccaaca aaacgctttc aatttttgta
   104941 gaactaaaaa agttgtgaca agtgacaaac tctccagtga atggtattct cctgccaact
   105001 cctcggtcac aagtcacaat attcaacaaa catgagaagc tgctttttga aattgttcct
   105061 tgtgactcgg agtgaaacgg caggcttacg acctctgggt aaacggtgtt caaaagccag
   105121 tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gctattttca caaaattttt
   105181 ctta
//
LOCUS       DACGLH010000002        93453 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.002, whole genome shotgun sequence.
ACCESSION   DACGLH010000002 DACGLH010000000
VERSION     DACGLH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.002"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            85..972
                     /locus_tag="IXT21_000096"
     CDS             85..972
                     /locus_tag="IXT21_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4867538.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IXT21_000097"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IXT21_000097"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ4867539.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IXT21_000098"
     CDS             2274..2720
                     /locus_tag="IXT21_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4867540.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IXT21_000099"
     CDS             2993..4345
                     /locus_tag="IXT21_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ4867541.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IXT21_000100"
     CDS             4516..4803
                     /locus_tag="IXT21_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867542.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="IXT21_000101"
     CDS             5029..5472
                     /locus_tag="IXT21_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4867543.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="IXT21_000102"
     CDS             complement(5478..6662)
                     /locus_tag="IXT21_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HAQ4867544.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFVGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="IXT21_000103"
     CDS             6930..7628
                     /locus_tag="IXT21_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ4867545.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="IXT21_000104"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="IXT21_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ4867546.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="IXT21_000105"
     CDS             complement(8438..8971)
                     /locus_tag="IXT21_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867547.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="IXT21_000106"
     CDS             complement(9268..11649)
                     /locus_tag="IXT21_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ4867548.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLTRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IXT21_000107"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IXT21_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ4867549.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="IXT21_000108"
     CDS             12350..12889
                     /locus_tag="IXT21_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ4867550.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="IXT21_000109"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="IXT21_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ4867551.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="IXT21_000110"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXT21_000110"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="IXT21_000111"
     CDS             13996..15162
                     /locus_tag="IXT21_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ4867552.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="IXT21_000112"
     CDS             15208..16704
                     /locus_tag="IXT21_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ4867553.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="IXT21_000113"
     CDS             16785..17468
                     /locus_tag="IXT21_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4867554.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="IXT21_000114"
     CDS             18113..19303
                     /locus_tag="IXT21_000114"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ4867555.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="IXT21_000115"
     CDS             19405..20358
                     /locus_tag="IXT21_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ4867556.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="IXT21_000116"
     CDS             20427..21776
                     /locus_tag="IXT21_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4867557.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="IXT21_000117"
     CDS             complement(21959..22282)
                     /locus_tag="IXT21_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867558.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="IXT21_000118"
     CDS             22411..23574
                     /locus_tag="IXT21_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867559.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="IXT21_000119"
     CDS             23644..24993
                     /locus_tag="IXT21_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4867560.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="IXT21_000120"
     CDS             complement(25038..25469)
                     /locus_tag="IXT21_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4867561.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="IXT21_000121"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="IXT21_000121"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ4867562.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="IXT21_000122"
     CDS             28699..29295
                     /locus_tag="IXT21_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4867563.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="IXT21_000123"
     CDS             29417..30565
                     /locus_tag="IXT21_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ4867564.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="IXT21_000124"
     CDS             complement(30603..31016)
                     /locus_tag="IXT21_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ4867565.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="IXT21_000125"
     CDS             31095..31526
                     /locus_tag="IXT21_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ4867566.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="IXT21_000126"
     CDS             complement(31625..31858)
                     /locus_tag="IXT21_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ4867567.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="IXT21_000127"
     CDS             32041..33711
                     /locus_tag="IXT21_000127"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ4867568.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="IXT21_000128"
     CDS             33858..34244
                     /locus_tag="IXT21_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867569.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="IXT21_000129"
     CDS             34231..34929
                     /locus_tag="IXT21_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4867570.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="IXT21_000130"
     CDS             34934..35398
                     /locus_tag="IXT21_000130"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ4867571.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="IXT21_000131"
     CDS             35395..36306
                     /locus_tag="IXT21_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ4867572.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="IXT21_000132"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="IXT21_000132"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ4867573.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="IXT21_000133"
     CDS             37094..38377
                     /locus_tag="IXT21_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ4867574.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="IXT21_000134"
     CDS             38390..39316
                     /locus_tag="IXT21_000134"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ4867575.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="IXT21_000135"
     CDS             39335..40615
                     /locus_tag="IXT21_000135"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ4867576.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="IXT21_000136"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="IXT21_000136"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ4867577.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="IXT21_000137"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="IXT21_000137"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ4867578.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="IXT21_000138"
     CDS             44940..45716
                     /locus_tag="IXT21_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ4867579.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="IXT21_000139"
     CDS             45713..46645
                     /locus_tag="IXT21_000139"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ4867580.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="IXT21_000140"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="IXT21_000140"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ4867581.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="IXT21_000141"
     CDS             47365..47994
                     /locus_tag="IXT21_000141"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ4867582.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..48296
                     /locus_tag="IXT21_000142"
     CDS             47982..48296
                     /locus_tag="IXT21_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867583.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48334..48975
                     /locus_tag="IXT21_000143"
     CDS             48334..48975
                     /locus_tag="IXT21_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4867584.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49006..49710
                     /locus_tag="IXT21_000144"
     CDS             49006..49710
                     /locus_tag="IXT21_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ4867585.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49707..50609
                     /locus_tag="IXT21_000145"
     CDS             49707..50609
                     /locus_tag="IXT21_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4867586.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50767..52473)
                     /locus_tag="IXT21_000146"
     CDS             complement(50767..52473)
                     /locus_tag="IXT21_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ4867587.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52663..53286
                     /locus_tag="IXT21_000147"
     CDS             52663..53286
                     /locus_tag="IXT21_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867588.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53736..54785
                     /locus_tag="IXT21_000148"
     CDS             53736..54785
                     /locus_tag="IXT21_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4867589.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54782..55663
                     /locus_tag="IXT21_000149"
     CDS             54782..55663
                     /locus_tag="IXT21_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4867590.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55666..56436
                     /locus_tag="IXT21_000150"
     CDS             55666..56436
                     /locus_tag="IXT21_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4867591.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56715..57569
                     /gene="aroE"
                     /locus_tag="IXT21_000151"
     CDS             56715..57569
                     /gene="aroE"
                     /locus_tag="IXT21_000151"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ4867592.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57747..58910
                     /locus_tag="IXT21_000152"
     CDS             57747..58910
                     /locus_tag="IXT21_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ4867593.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59027..59437
                     /locus_tag="IXT21_000153"
     CDS             59027..59437
                     /locus_tag="IXT21_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ4867594.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59611..61839
                     /gene="topB"
                     /locus_tag="IXT21_000154"
     CDS             59611..61839
                     /gene="topB"
                     /locus_tag="IXT21_000154"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4867595.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61977..62294
                     /locus_tag="IXT21_000155"
     CDS             61977..62294
                     /locus_tag="IXT21_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867596.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62454..62690
                     /locus_tag="IXT21_000156"
     CDS             62454..62690
                     /locus_tag="IXT21_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4867597.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62687..63085
                     /locus_tag="IXT21_000157"
     CDS             62687..63085
                     /locus_tag="IXT21_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ4867598.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63752..64696)
                     /locus_tag="IXT21_000158"
     CDS             complement(63752..64696)
                     /locus_tag="IXT21_000158"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ4867599.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64992..65936)
                     /locus_tag="IXT21_000159"
     CDS             complement(64992..65936)
                     /locus_tag="IXT21_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4867600.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66157..67371
                     /locus_tag="IXT21_000160"
     CDS             66157..67371
                     /locus_tag="IXT21_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4867601.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67393..69408
                     /locus_tag="IXT21_000161"
     CDS             67393..69408
                     /locus_tag="IXT21_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ4867602.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69491..70864)
                     /locus_tag="IXT21_000162"
     CDS             complement(69491..70864)
                     /locus_tag="IXT21_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ4867603.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70890..71174)
                     /locus_tag="IXT21_000163"
     CDS             complement(70890..71174)
                     /locus_tag="IXT21_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4867604.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71176..73191)
                     /locus_tag="IXT21_000164"
     CDS             complement(71176..73191)
                     /locus_tag="IXT21_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4867605.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73370..73675)
                     /locus_tag="IXT21_000165"
     CDS             complement(73370..73675)
                     /locus_tag="IXT21_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ4867606.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73764..74795)
                     /locus_tag="IXT21_000166"
     CDS             complement(73764..74795)
                     /locus_tag="IXT21_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ4867607.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75002..75877
                     /locus_tag="IXT21_000167"
     CDS             75002..75877
                     /locus_tag="IXT21_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4867608.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76029..76871)
                     /locus_tag="IXT21_000168"
     CDS             complement(76029..76871)
                     /locus_tag="IXT21_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4867609.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76963..78840)
                     /locus_tag="IXT21_000169"
     CDS             complement(76963..78840)
                     /locus_tag="IXT21_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4867610.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78837..80258)
                     /locus_tag="IXT21_000170"
     CDS             complement(78837..80258)
                     /locus_tag="IXT21_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4867611.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80261..83650)
                     /locus_tag="IXT21_000171"
     CDS             complement(80261..83650)
                     /locus_tag="IXT21_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ4867612.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83907..85298
                     /locus_tag="IXT21_000172"
     CDS             83907..85298
                     /locus_tag="IXT21_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4867613.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85371..86018)
                     /locus_tag="IXT21_000173"
     CDS             complement(85371..86018)
                     /locus_tag="IXT21_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4867614.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86026..86711)
                     /locus_tag="IXT21_000174"
                     /pseudo
     CDS             complement(86026..86711)
                     /locus_tag="IXT21_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(86717..87790)
                     /locus_tag="IXT21_000175"
     CDS             complement(86717..87790)
                     /locus_tag="IXT21_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ4867615.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88012..88686
                     /locus_tag="IXT21_000176"
     CDS             88012..88686
                     /locus_tag="IXT21_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4867616.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88691..90031
                     /locus_tag="IXT21_000177"
     CDS             88691..90031
                     /locus_tag="IXT21_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4867617.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90180..91166)
                     /locus_tag="IXT21_000178"
     CDS             complement(90180..91166)
                     /locus_tag="IXT21_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ4867618.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91890..92921
                     /locus_tag="IXT21_000179"
     CDS             91890..92921
                     /locus_tag="IXT21_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4867619.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93030..>93453
                     /locus_tag="IXT21_000180"
     CDS             93030..>93453
                     /locus_tag="IXT21_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ4867620.1"
                     /translation="MMETITYSLLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        29895 a        16574 c        18964 g        28020 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaacaa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggtta agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaatt aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48241 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48301 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48361 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48421 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48481 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48541 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48601 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48661 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48721 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48781 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48841 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48901 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48961 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    49021 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    49081 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    49141 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49201 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49261 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49321 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49381 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49441 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49501 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49561 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49621 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49681 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49741 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49801 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49861 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49921 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49981 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    50041 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    50101 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    50161 gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50221 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50281 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50341 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50401 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50461 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50521 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50581 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50641 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50701 aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc tagaaacact
    50761 cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg tcacgataat
    50821 cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt ttcggatatg
    50881 ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg aaaaataggc
    50941 agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt taatgatgac
    51001 gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat
    51061 tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc cgtcagaaga
    51121 aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt tcttcttttt
    51181 caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg ggccggcaat
    51241 ttccagttga gataagacag attcaagata ttggatttca tctttggctt cttggatttg
    51301 ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat atttctgtgc
    51361 attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat cttcttcata
    51421 ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag
    51481 tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg tttgttcacg
    51541 ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa tcagttctcc
    51601 gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat ctaataattg
    51661 actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct gataagcaat
    51721 cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct gatggctgat
    51781 ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca gtaatctttt
    51841 ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg
    51901 atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc taaatgggtt
    51961 caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag aacgataagt
    52021 gttttgagag cttccaatgt gtttgatcgc atccagtatt tttccagttt ctcgattaac
    52081 taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat tttgtagatc
    52141 acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt cgatttgttc
    52201 gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga agttaggcgg
    52261 attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac ttgggtgcgc
    52321 agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga tttcattttc
    52381 ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca attcgttgac
    52441 catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc tttttataag
    52501 tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag
    52561 tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg aaatccaaat
    52621 ttttaggtat aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa
    52681 gtagttaaat cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat
    52741 ccaagtaagt attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt
    52801 gcaatgtttt tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg
    52861 tccataccaa ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa
    52921 caaagccggg cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg
    52981 cttgatgttt ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat
    53041 ccgatcgttt atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg
    53101 acatttattt tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga
    53161 ataactgatt ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc
    53221 aaacaattag aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct
    53281 aattgagaca ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga
    53341 acaagtgatg tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa
    53401 ttcaaaaatt cgattagact taaaacaatt cttaaggaaa gggtgacctg taatgaactt
    53461 atcaaaacgt atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt
    53521 attaatggtg agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat
    53581 ctatgctaga catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta
    53641 aagacaatga ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata
    53701 ataaataaaa agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag
    53761 ttgtagtaat tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg
    53821 aaaaagaaag tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat
    53881 acgtgagcca tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag
    53941 aaggattaaa agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga
    54001 gcaagctagc cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa
    54061 tcgcaacacc tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag
    54121 gtgctgtaac ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta
    54181 atgtaacagg tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt
    54241 tgttgccaaa tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat
    54301 accaagtagc acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg
    54361 cggtgccttc cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt
    54421 ttatctatgt tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag
    54481 caaacaaagc aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac
    54541 tagcaacaat cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag
    54601 aacttgcgag tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg
    54661 atacagtgat caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga
    54721 aggaaaaagc caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca
    54781 aatgatcgta tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt
    54841 catgacttat cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg
    54901 tgctgctgtc tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt
    54961 aggcgtaggt gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa
    55021 gataccggtt attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt
    55081 tgtgatgcga acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca
    55141 tcgctttggg ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat
    55201 cttgattgct gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac
    55261 aggagacaac gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt
    55321 aggtttaact ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga
    55381 cggatatgca gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat
    55441 catcggtgaa gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt
    55501 cggcagtatc atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac
    55561 ttatctgaag ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa
    55621 gaaagcactg aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg
    55681 acaatcaaaa atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg
    55741 gatgaaatta accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt
    55801 gctggaaaaa gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg
    55861 atctctttta aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt
    55921 tctcgagttt tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat
    55981 ttgctacttg cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat
    56041 caaaaagaag attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg
    56101 gatactccag cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg
    56161 acgatcacag ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa
    56221 acagctaaag cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt
    56281 ctcatgatca cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc
    56341 caaaaaggga aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc
    56401 gaccttttgc agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag
    56461 tagaaggact tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata
    56521 aacaattaaa catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa
    56581 atagaatatt ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc
    56641 tagacgtttc gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac
    56701 aaaggaggaa taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg
    56761 cttctcacag tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg
    56821 cggtgtattt agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc
    56881 gaacttttga tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt
    56941 atctggatga ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg
    57001 aaaagggtcg tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag
    57061 aagctgggat ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa
    57121 aggcagtcac tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa
    57181 acaaccaaac attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt
    57241 gtaagataaa agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa
    57301 gctgtcttct agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc
    57361 tcttagatat aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta
    57421 gagaaacacg tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg
    57481 gcatgttgat ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga
    57541 tccatgtgat tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct
    57601 cgcataaacg gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt
    57661 tttattaaat tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta
    57721 tcagtaagaa acgaggagga accacaatga gtcggatgga tcgctataag gacatccata
    57781 agaaggcgaa acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac
    57841 gaacgcgtat ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat
    57901 ttgaacattt gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac
    57961 aacaaaaaaa taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa
    58021 gaaagcgatc gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag
    58081 gtatctcatt ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga
    58141 cttcagaaac ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg
    58201 gaagtgatac acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg
    58261 atggtccttc ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc
    58321 caggggtagg acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc
    58381 gtcaaacttt agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca
    58441 aatcgtttga aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa
    58501 aagatctgaa tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg
    58561 ttcttttgca gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac
    58621 gccaacagca agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc
    58681 gtgcacctta tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt
    58741 tcttgacgaa aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg
    58801 tgccagtaga cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa
    58861 ttgataatca aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa
    58921 gatcaagaag aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa
    58981 aaacgctttc ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct
    59041 gaacttcaaa aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga
    59101 agcatcaggt atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga
    59161 agaagcaaaa aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt
    59221 tgcagatacg ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt
    59281 catccagaca agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg
    59341 taagattgag atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga
    59401 tcgatttggc atcaactggc agattaacca agagtgacag ctaaagatga atattttctg
    59461 gaaacatcaa gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg
    59521 actatacggt atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc
    59581 cgcaaaaaat taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct
    59641 tcacaagcaa aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat
    59701 gaaatcagcg atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg
    59761 gtagatatgg ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat
    59821 ttgcctattt tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt
    59881 tcgatcgtaa aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat
    59941 cgagaaggtg aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa
    60001 aagaactatt tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt
    60061 aagaatctcc aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag
    60121 aacgctgatt ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga
    60181 aaagggattg atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt
    60241 tatcagctgc aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt
    60301 ttaattacga caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa
    60361 acccaagaag agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac
    60421 ggacggattc ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg
    60481 ctatctagtt tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt
    60541 gatgctgctc aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt
    60601 tacattacag aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt
    60661 ttaggaatag aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg
    60721 aaaaaagttc aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc
    60781 tttgcgaagc tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg
    60841 gctatgtttg ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa
    60901 ttaaagctaa aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc
    60961 atcaagtcaa aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca
    61021 gaagtagaac tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga
    61081 acgttgatca cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa
    61141 attctcaaag aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat
    61201 cttaagcaga agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga
    61261 atcactcttt gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg
    61321 cgctgggaag ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta
    61381 gagaatatca aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag
    61441 gtagactttt cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac
    61501 gatcaattag gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc
    61561 gctacatgct tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag
    61621 ctgactaaaa cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa
    61681 ggttttactg gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca
    61741 atcggttttt cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa
    61801 gaaacatcaa gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg
    61861 aatatttatt ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat
    61921 caagcaaaaa cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga
    61981 aaaaagtgca ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc
    62041 aagcatttct aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa
    62101 cagcatattc accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt
    62161 atttttttca taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg
    62221 tggtggtagc ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc
    62281 aatattttgg ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt
    62341 tgtcattttt tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac
    62401 ttggatataa tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa
    62461 aaatgaaaaa gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag
    62521 aagtaaaaga attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg
    62581 atggttctgt acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt
    62641 cgatcatgaa tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc
    62701 gactaaagaa attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat
    62761 gatcggtgta aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt
    62821 attcaatcaa gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt
    62881 agctaaaaaa catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt
    62941 gtttcttcaa ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat
    63001 cataatgatt accactagtt cattggattt cgattcacta aagtccaaga taaccagtta
    63061 tttacgacaa accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc
    63121 atgtcattaa ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata
    63181 aaaatatgga aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt
    63241 gaatagtagg aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac
    63301 ataatatata ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt
    63361 atcaaaaatc gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc
    63421 agtttttctt actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt
    63481 taatcaaata tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt
    63541 taattaacca cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt
    63601 catatgttca tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt
    63661 tgcttaccta ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt
    63721 tttaggattt ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta
    63781 aagcacttgt tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa
    63841 cttcacgaac accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt
    63901 cgtattcacc agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag
    63961 cttttacgat acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa
    64021 tgatctcata agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa
    64081 tacgatggtc tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc
    64141 atgcagcaac ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt
    64201 caacttttaa tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag
    64261 taccgataac acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat
    64321 ctactgggtt tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta
    64381 cgatttggcg catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt
    64441 tttggttgat accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac
    64501 ctgaccatac agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt
    64561 ccccttctgc tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt
    64621 tgatcattgc ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac
    64681 gacttgtttt tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa
    64741 agtatagtct tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt
    64801 tttgatggaa acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat
    64861 atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc
    64921 aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa
    64981 agattttctt cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg
    65041 aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac
    65101 gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt
    65161 tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac cggattgtag
    65221 ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct
    65281 aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct
    65341 aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta
    65401 tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt
    65461 gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt
    65521 atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat
    65581 aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt
    65641 ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct
    65701 ccattaataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt
    65761 gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga
    65821 aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca
    65881 ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa
    65941 aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct
    66001 tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt
    66061 ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact
    66121 atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg
    66181 aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca
    66241 attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg
    66301 actgctccct cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa
    66361 atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc
    66421 cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc
    66481 tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa
    66541 cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca
    66601 tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc
    66661 agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt
    66721 caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt
    66781 tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc
    66841 acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc
    66901 atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat
    66961 cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact
    67021 gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta
    67081 ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc
    67141 aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg
    67201 ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt
    67261 atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag
    67321 acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg
    67381 aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac
    67441 tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg
    67501 ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt
    67561 cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat
    67621 ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt
    67681 atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa
    67741 tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg
    67801 ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat
    67861 ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat
    67921 ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga
    67981 gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca
    68041 agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact
    68101 ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt
    68161 tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta
    68221 tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat
    68281 gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga
    68341 acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac
    68401 tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa
    68461 tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg
    68521 aatgaattag ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa
    68581 atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc
    68641 atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact
    68701 catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat
    68761 gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat
    68821 gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta
    68881 aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt caaattctat
    68941 gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat
    69001 tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact
    69061 caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat
    69121 gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca
    69181 aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat
    69241 tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg
    69301 attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta
    69361 gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg
    69421 cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt
    69481 tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa
    69541 agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac
    69601 cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg
    69661 tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa
    69721 tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc
    69781 cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg
    69841 tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa
    69901 agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt
    69961 tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca
    70021 ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca
    70081 gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag
    70141 ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat
    70201 ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc
    70261 caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg
    70321 caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag
    70381 ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc
    70441 ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa
    70501 gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta
    70561 gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat
    70621 tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta
    70681 gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga
    70741 tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa
    70801 tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat
    70861 ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt
    70921 tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata
    70981 gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat
    71041 acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca
    71101 agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact
    71161 gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt
    71221 tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc
    71281 caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg
    71341 atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac
    71401 ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat
    71461 aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt
    71521 attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg
    71581 atccgataat tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc
    71641 cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc
    71701 tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg
    71761 cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc
    71821 caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag
    71881 aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc
    71941 tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat
    72001 caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc
    72061 ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc
    72121 ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc
    72181 gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga
    72241 ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg
    72301 aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca
    72361 ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca
    72421 aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac
    72481 ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc
    72541 catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat
    72601 tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac
    72661 tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc
    72721 aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga
    72781 ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa
    72841 ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc
    72901 ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc
    72961 tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc
    73021 ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa
    73081 aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc
    73141 tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt
    73201 cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac
    73261 ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa
    73321 aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat
    73381 catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt
    73441 cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg
    73501 aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt
    73561 cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt
    73621 taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg
    73681 ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa
    73741 caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt
    73801 ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg
    73861 ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa
    73921 attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt
    73981 tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct
    74041 tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt
    74101 ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa
    74161 aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag
    74221 ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt
    74281 ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg
    74341 aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact
    74401 ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga
    74461 cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt
    74521 ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat
    74581 tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc
    74641 tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt
    74701 tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta
    74761 ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt
    74821 tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt
    74881 tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa
    74941 atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat
    75001 catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat
    75061 tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc
    75121 aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag
    75181 cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt
    75241 caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc
    75301 aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca
    75361 tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc
    75421 aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa
    75481 gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag
    75541 cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat
    75601 gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga
    75661 aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa
    75721 agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt
    75781 gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat
    75841 gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata
    75901 agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc
    75961 tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg
    76021 aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg
    76081 aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat
    76141 actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca
    76201 agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc
    76261 cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact
    76321 tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc
    76381 ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt
    76441 gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc
    76501 agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa
    76561 ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta
    76621 gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc
    76681 atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa
    76741 tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct
    76801 agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc
    76861 tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat
    76921 ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt
    76981 gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac
    77041 atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc
    77101 aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg
    77161 gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata
    77221 ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc
    77281 tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt
    77341 gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac
    77401 gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt
    77461 ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc
    77521 tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga
    77581 aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata
    77641 atttactggg tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga
    77701 atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt
    77761 gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg
    77821 aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt
    77881 agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa
    77941 aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa
    78001 attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc
    78061 ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt
    78121 ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct
    78181 tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc
    78241 agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag
    78301 atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat
    78361 ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc
    78421 ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac
    78481 atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac
    78541 tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt
    78601 gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat
    78661 ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag
    78721 gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag
    78781 taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat
    78841 tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata
    78901 ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta
    78961 ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa
    79021 gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc
    79081 tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg
    79141 gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc
    79201 tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct
    79261 ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt
    79321 gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt
    79381 gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc
    79441 aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct
    79501 tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct
    79561 tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc
    79621 gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat
    79681 accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata
    79741 gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc
    79801 ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt
    79861 tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta
    79921 gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt
    79981 gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa
    80041 acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg
    80101 tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc
    80161 tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac
    80221 aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt
    80281 ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact agatagattc
    80341 ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta
    80401 aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca
    80461 cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc
    80521 cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa
    80581 aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc
    80641 tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa
    80701 acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt
    80761 ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag
    80821 tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat
    80881 caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat
    80941 ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc
    81001 cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca
    81061 actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag
    81121 aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg
    81181 tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg
    81241 ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag
    81301 cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg
    81361 tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta
    81421 caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta
    81481 gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac
    81541 gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc
    81601 aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat
    81661 cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag
    81721 ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag
    81781 ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga
    81841 ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct
    81901 tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac
    81961 tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt
    82021 gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat
    82081 ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt
    82141 gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt
    82201 ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg
    82261 ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga
    82321 ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt
    82381 aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac
    82441 catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg
    82501 catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt
    82561 ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat
    82621 atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg
    82681 ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt
    82741 tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta
    82801 catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat
    82861 actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg
    82921 tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt
    82981 tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat
    83041 aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca
    83101 cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg
    83161 atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt
    83221 ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt
    83281 ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga
    83341 tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt
    83401 ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg
    83461 tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt
    83521 cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg
    83581 gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg
    83641 tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt
    83701 attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa
    83761 gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt
    83821 ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca
    83881 ggttgttgga acgaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg
    83941 acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg
    84001 taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac
    84061 aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg
    84121 aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta
    84181 aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt
    84241 tgaaacaatg caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt
    84301 ttcagttaac tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt
    84361 taagttacta cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa
    84421 ttatggtcaa gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat
    84481 ctttctttat tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg
    84541 aatggaaagc tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa
    84601 tcttgaaaaa tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt
    84661 ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga
    84721 tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag
    84781 aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata
    84841 cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac
    84901 gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc
    84961 tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc
    85021 attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat
    85081 atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg
    85141 ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga
    85201 atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg
    85261 caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta
    85321 taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt
    85381 ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa
    85441 ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa
    85501 ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat
    85561 ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt
    85621 cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac
    85681 cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt
    85741 aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc
    85801 aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct
    85861 gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt
    85921 ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct
    85981 ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt
    86041 ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat
    86101 acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag
    86161 taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa
    86221 gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc
    86281 agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga
    86341 atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc
    86401 ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa
    86461 gcgtaaatct gctcgttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt
    86521 tctccggaag tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa
    86581 ccagaaggac caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc
    86641 aaagctgaga cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat
    86701 atttttttca tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac
    86761 gtatcgaaaa caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc
    86821 attgtgacca aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag
    86881 caaacaaaag agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag
    86941 atttagcgat gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct
    87001 tctgaagtgt catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa
    87061 agtatatcat cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga
    87121 atgtcccaat agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg
    87181 aatcctttat tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc
    87241 catacttcaa agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat
    87301 aagtttcagg aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg
    87361 tatctccaat tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg
    87421 ttttaaattc atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga
    87481 aaactgtaac attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg
    87541 ctttctctcc tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata
    87601 cttgatttgc atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt
    87661 taaactcttc tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca
    87721 agaacataat accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag
    87781 ctagaaacat ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg
    87841 aatagtgtaa ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat
    87901 tgtctaagac atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt
    87961 atttatgctt tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa
    88021 ctattagtag tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca
    88081 atcggcgaga taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga
    88141 gtttatgatc ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta
    88201 gcaaaaatac ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt
    88261 ttggaagata agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc
    88321 tatcgagaag aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt
    88381 gatgaaaatg ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg
    88441 tataaagaac aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta
    88501 caaaataagg gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat
    88561 tctgatacga cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga
    88621 agtactggat tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg
    88681 gtgtgatgca atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa
    88741 tgtagcagca tttgctttta tcttttttct tttgggattc attactttac agatattaaa
    88801 ccaatctgca tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac
    88861 agtacaatta gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac
    88921 ggaacaaaac agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa
    88981 cactcaagtg atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg
    89041 gcgtttcaac caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa
    89101 tattgaatta aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa
    89161 agcggagacg aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat
    89221 caagaattca ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat
    89281 tctctctatt ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc
    89341 ttggagaaga caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac
    89401 gattattcaa aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga
    89461 atccgaaagc atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga
    89521 tttgctgaca atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa
    89581 tacacaagaa tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg
    89641 aaaagagttt attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat
    89701 ccatcaagtt ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa
    89761 gatcattttg gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg
    89821 cccttctatc agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca
    89881 atctcgggcg aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat
    89941 cgaacagcat catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa
    90001 agtccaattg ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa
    90061 aaatcttgtc acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg
    90121 gaaataagcc gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct
    90181 tacgattttt tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct
    90241 tctcggctaa catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca
    90301 acttcttcac gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata
    90361 gtaatcgtgt catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt
    90421 ttgcgttcga tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg
    90481 accacatgtt tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat
    90541 ttttcttctt ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca
    90601 gtcttgcttg tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt
    90661 gtcatattca atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga
    90721 tctgttccag tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct
    90781 tgtgtatcca ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca
    90841 aattttccat cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc
    90901 gtctcagaat cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg
    90961 tcttcttcac catccatccg attttcatct gtttcaacat ctataccaga caatccgtca
    91021 aatcctagtc tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac
    91081 ccttcgttac gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg
    91141 atgaccgtta aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg
    91201 tatattgtta cttataaatt tattctatag gagacgattg ttttctacaa atatttagat
    91261 tggaatttga ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa tgaaagtcat
    91321 attctaataa aatgtcattt gtttctatat gaatttttat gaaaaatttc atataatttc
    91381 cactcgttgt gatggtttaa tttatccaat ccatcaataa gcaaacaact tatataaaaa
    91441 aggattaatt ctcaatataa ttgctcctac catagaataa atcttcatgt aaagtaatga
    91501 agtaccaact atttctaata cttctttaag aattatccca acaatatcgt tagacaaacc
    91561 attattgaat aaatggagag ccaatcgatt ctgaaaaaat gttttctaat ttttcggaaa
    91621 taaaagaaag agcctcctgc cattagaacg aaccaaatcc atttcattgc tcatgaagta
    91681 accacatagc taaaaagagg ataaatcaat taaaagatca aaagaatgac tgcactaata
    91741 aaataactaa aaatgtatct tcttacttca tatcttttag ctagtactta gttaaaaatt
    91801 tgcttcctaa gatcaagaca aactgtctat cttcatttga tgaaatgcgg taaaatatag
    91861 aaaatgaaca ctattggagg gaatttgtaa tggacagcaa aactagaaaa caactgcaag
    91921 aaaagttaaa gcaacgtaca cacttgctcc cttccgtctt tcaacagttt ttagaagaaa
    91981 atcgttcttc cctttcatta ggcagtcgct atgaatacgc aaaagatttt catctgttta
    92041 ctgattattt gagaaaagta ttttctgaag aagtggatga tcagctgatt atccatttag
    92101 aaaaacaaga ttatcagaat ttcttagcta aaatctatcg acatactcgt tcgttccaaa
    92161 ctacagcgaa ccaagaaacg gagcaattgt ttgaaaatag ctattacggg atttcaagga
    92221 agcaatattc attaaatcat tttttacgat ttttgtatca aaaaggactg attgaagaag
    92281 agatttctct tttgggtaca agtttaccgg aaacaaccaa agcagctccg cgatatatcg
    92341 atgcagaact tttcaaagat tttgagcatt tgtttatccc gattgaaggc tttcaaacaa
    92401 gcagagaagc tagctttgaa gaaaagaata gaccgaggaa ccttgcaatt actgccattc
    92461 ttctgtattg cggattaaag atccatgaag tagtagaact taaacgtaaa gatgtatctt
    92521 tgaccaagca gattcttaat ctgaatagaa aagaaaacag actgaataaa gtaccaatac
    92581 caaaagaagc gatgccattt ctggagaact attttaagtt aacagcacaa aatacggacg
    92641 aaaattcttt tgtttttcgt tcctcacatg atcagcaaat ttctcctcgg actattcgcc
    92701 aaattttgag caaaattaca gtatataaaa acgtatcgcc agaactatgc agaaaaacat
    92761 atcgttatta tgcagaacta tatttagatg atgctgacgc ggtaaattat ttatgtggta
    92821 atcgatacct ctcatcccat tcttttcaag atatctggga aaagatggtt gatttttctt
    92881 atcacgaatt agcggcattc gttcgggttt cttccatata aaacaaatgt atgatctttt
    92941 tttcacaata aactatgtta ttatataaag gattatgtta cgattatctt gaatttgtat
    93001 attacttaac aaaagataat ggagtgtgaa tgatggagac cataacgtat tcccttttga
    93061 acttatttac gaattttcaa gaagctgcag ctaacttttc cgagaccccg attatctttg
    93121 atgagccact ttcagctttc ccagaattag gacttgaaac aacttacaaa aaatgctgcg
    93181 aaagtgtgtt gaaacgggct tatcaattgg ctcatcttgg agtgaaatcc ggagacaaag
    93241 ttattattta taagagttcc gcttttgaca cataccttct agctgtttct gtgtcttacc
    93301 ttggtgcagt gcctgtcatg acttcttatc atctaccaac aacaacaatg gaagtattta
    93361 tcgatcgttt agaagaccct ttcattttat ttgatgatga gacaaaaaag cgagtgagag
    93421 aaatttcaaa tggaacaaaa tcaaaacaga ttc
//
LOCUS       DACGLH010000003        91311 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.003, whole genome shotgun sequence.
ACCESSION   DACGLH010000003 DACGLH010000000
VERSION     DACGLH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.003"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(178..1488)
                     /locus_tag="IXT21_000181"
     CDS             complement(178..1488)
                     /locus_tag="IXT21_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ4867621.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1500..2918)
                     /locus_tag="IXT21_000182"
     CDS             complement(1500..2918)
                     /locus_tag="IXT21_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4867622.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3158..4726)
                     /locus_tag="IXT21_000183"
     CDS             complement(3158..4726)
                     /locus_tag="IXT21_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ4867623.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4730..5140)
                     /locus_tag="IXT21_000184"
     CDS             complement(4730..5140)
                     /locus_tag="IXT21_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ4867624.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5137..5505)
                     /locus_tag="IXT21_000185"
                     /pseudo
     CDS             complement(5137..5505)
                     /locus_tag="IXT21_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
     gene            complement(5591..6334)
                     /locus_tag="IXT21_000186"
     CDS             complement(5591..6334)
                     /locus_tag="IXT21_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867625.1"
                     /translation="MTEIQIKNLIKEYEKEYIEFMEIEKLPQYKIDFFEINVEESDAA
                     GFASAAQAYYNTKTDEHILRICKSSEIPRYIVFHEFTHILDTEMYAKQDSWKYMALSG
                     YTEYHAAQVELMIMLGADSIQTQDFSFTVDVEIGNSTVRNYLNSRHQLVVNMMNRTDF
                     PRDIEALKTTVGVLYNYFGVRSICKMYAKDYTEEVDNTIIIQKLSKVLFEEINSFMVG
                     WFNEAQVELSFVSYMKIMWPMLQSYFGKE"
     gene            complement(6475..6840)
                     /locus_tag="IXT21_000187"
     CDS             complement(6475..6840)
                     /locus_tag="IXT21_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867626.1"
                     /translation="MIEENQRKSKEKIELALQAIQDMLANKERISVPKLMKKTGLSRG
                     FFYKNPTVRDTLNQAVEQQAGMIDPRREILNMAMEKQIELLNQKVAALSRENKELKRK
                     NEKLQKALRKQDLNFIKNL"
     gene            complement(6833..8332)
                     /locus_tag="IXT21_000188"
     CDS             complement(6833..8332)
                     /locus_tag="IXT21_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAQ4867627.1"
                     /translation="MENDIIYFSDFPNLQETGTRKDNGKFDLTLLPTQELKEEFRGYI
                     MYRCKNGTFRALIQDRTAYNHIAKFLNSRINRRIKSLGDRNPEKWISLLKGWMLEQGI
                     TIVKEKKSVYGTVSYGEAVTILYFRNVLKFLGPEDLRDEIEKDVWELKNLDIKIRSNP
                     IYNVKTLDFRKIYQPDIREECKKAVYMNLQYEAIGTVQGELTIMRIFSEYLQKEYSKI
                     KSCSEIDREVLEEFLIHLSTKDTSHSANSSYVISLRRQLETIGKIYSYERLEHLFINT
                     DIPPEVNAEFRVYSDDEMKRLNAEITQMDVQIARCLLIHQMLGTRISDTLTLRPDCLT
                     RENGQDMIEIYQVKTKRYKKPISKELAKLLQSSIEYTQEKFGDTEYIFVNEKEPDRPM
                     QYMAIKTKVMSMIQEKQLKDDHGELFGFGTHMFRHYYGVKLTEMHLDDWTIARLLGHK
                     RLNNVQHYRKMSNQRMADETREVRQRMSDIIYMSLARWGEEYEQIRQDD"
     gene            complement(8322..10067)
                     /locus_tag="IXT21_000189"
     CDS             complement(8322..10067)
                     /locus_tag="IXT21_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4867628.1"
                     /translation="MPIYLLQQAEHITESEVDIEKKKQELYSEIDALGIKSDSRINKL
                     KKFLADRKIWHLEEMDYPLRNSYEQYLRGQIRSQIVSFYLKIYDTVKQQHIYQQMQTL
                     NGKRIYEWKYQNKIYFLKYYPEKEIAETFETSYKTNLLVWDFTQNCSEVLKKQIFYTL
                     SRIIANTSMSKAYRNVRLKSLKLLYDSCVQLNITDIGLLEMEQVETILKNFPETSQRS
                     ILGECRRDAFMQQEQIQWEANVWYLERLHLGKHRIDESKSLISISFMEVKEIQNREIL
                     QAYMKYELGITGQAVSTIVRRFVCIRNFIELLEQEKILAIHATVAEVKKYADGLRERG
                     IQAKGFNERIFGIGHFYKFMEVKQYITRMPFRIEYFQQKEVIVHHDRSVEETVYMEIL
                     KKLYLFPERLRCMFLHLWCLGLRASEVCTLKGNAYYQQGEDYWIQVYQVKMKNYKRIP
                     IPQALYQIMKVYLKKHEIQPEEFIFKNSRGGACLYGTFRTQMIEACRENKIANGEYLF
                     QSHDYRHTVATMFYDSHVSLQSIRDYLGHTYEEMTRQYIDYMPQRIAKANDEFFEMQG
                     SSLASWLKKEEKDGK"
     gene            complement(10081..11205)
                     /locus_tag="IXT21_000190"
     CDS             complement(10081..11205)
                     /locus_tag="IXT21_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4867629.1"
                     /translation="MIYKVETIKDGTEKYFFIRNLETMSIEELPSKYLMHKIKCKRSP
                     NTVKRTAFSICYYMKYMAEKEMELTEVYQLDYEKQTEHFVEFLYWLKAGNHTEQTAGE
                     KKCPNEGTCNAYLKDVFRFYLFIEAEYEQYGSLKTLSYNQIIAVNQVGVKKVLRNHSF
                     KAYLKEEEHRGRTAKENQIITILQACTNRRDQLLLLMLAETGYRIGELLGVKYVKDID
                     YRNHMIRVCFREDNENEARAKNAEYRSAKISNDTFEFLMDYLAKYRKILQHQDYLFVN
                     IMGENIGKPLRVDSVYDMLDRMEKKTGVKITPHMLRHYFANMRKQAGWELEMISQALG
                     HKHLDTTIKYLDWLDDELIEASNEFYAQHTAIYGAERLLE"
     gene            complement(11361..13328)
                     /locus_tag="IXT21_000191"
     CDS             complement(11361..13328)
                     /locus_tag="IXT21_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ4867630.1"
                     /translation="MYYTREYLNRAHREIDTIFRVLFPEHGMAVREEQIMLCHKMLDN
                     LLGRNIALCDAGVGIGKTYAYLVACVLMRKYSLLAEGCSPYEQRPVVISTSSIALQKA
                     ILTEYIPFLSRILQENGTIQAPIKAVIRKGKEHFVCDERLEHRIVAIEEKNKNALQKE
                     ALLSLKEHYDMDEVSNLSGFDRRMVSVPKFCSGDCPKRGSCRYQQYLERSRDHEMFIQ
                     ICNHNYLLADGYHRLQDYRPLLKDYRALIVDEAHKLPDAAKQMFGKSLCYDDIREICF
                     YLGNEYQGPEIRKLSGTIRMVLDIIGENHRTRYGIKEEFHMTEECAMYLYEGIQTMNK
                     IIEKLEKKIPKWIRNKLEETKSVLECFFHQDKKYVLHLKQDHDHRIILCASSRRIPQY
                     LDQMLWSRGMGAILTSGTLKTGQGFSHIRKMTGLQRVRRVREYVADSPFEYQKNCLLY
                     LPKNLKTCRRESQEEAEMIAGHIHSLICSTYGHTLVLFTSYHLMGNVYQMLRDEIPFP
                     MIEVWRHSPEEITRFKQMDNAVLFAAGSCWEGVDFPGDMVSSLIIVKLPFAVPDPISE
                     AQKKEYASLKDYIQAVIVPDMQKKLRQGFGRALRTETDTCVVSILDERAGEGGRYHEE
                     VMCALPSCKMAKDIKDVQHFIRNRKGVEYYL"
     gene            complement(13863..14297)
                     /locus_tag="IXT21_000192"
     CDS             complement(13863..14297)
                     /locus_tag="IXT21_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4867631.1"
                     /translation="MAYNKTSEERKWNYWKEQEEKKLRELGMEEEKIQKLRQMDREDF
                     LEERRYREHLDETMQDMDSIKEKDSELFARDVKELLDHIGDKRIYELLKETEHQTLEI
                     LLLSTWGYSGREVAKIMGMAEQTIYTKRNRLRKKLKNLSKIE"
     gene            complement(14625..15251)
                     /locus_tag="IXT21_000193"
     CDS             complement(14625..15251)
                     /locus_tag="IXT21_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867632.1"
                     /translation="MQKKNCVLCISIGIIVCILLSACSRQPDFDAKSYVQSSLDAHYH
                     GEYKDYANLLEISEKDAKKEIEEDFNESIQQQFDDSDNITDKGIADYAEKLTEVKKLA
                     KYKVQDVKEEDGVYTVSVQVEPSNVFQTLQQSSTEVSNEKIKQGLDGNDPEVFAAVLT
                     ESVQKSLEKNRYGKAVTVNVSVEKDNSGKYGLLDTEMNKLEAAMFPTE"
     gene            complement(15704..16129)
                     /locus_tag="IXT21_000194"
                     /pseudo
     CDS             complement(15704..16129)
                     /locus_tag="IXT21_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
     gene            complement(16682..25552)
                     /locus_tag="IXT21_000195"
                     /pseudo
     CDS             complement(16682..25552)
                     /locus_tag="IXT21_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rhs family protein"
     gene            complement(25744..27018)
                     /locus_tag="IXT21_000196"
     CDS             complement(25744..27018)
                     /locus_tag="IXT21_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4867633.1"
                     /translation="MNIHLITYLTSKLFWIYTVFRYNTILYPKNEDTQKLFAYFCAYL
                     LSCWGSMQWGTMYGDLIFELIALLIITVAYQADWQHHVWNTLMVYIMGILCDGIPIVF
                     FQRYAVARNPVHHHMGPGTIVMGNFIFFLLEVIIERKVGVKRSYYIKNRHWLAMLSVP
                     VISIIVCIRILLPDRRGVRSTIVLFLLFINVFIFYLHDEVQKSYIETAKQEEMKIQMS
                     QYAKELEMMMKSQDRLNKIHHDYKHHLTTIGAMAKTGGNEEILSYLKQMENIAGTALC
                     HHLYTENRNMNNLLNYILEDSKSVIENPEISVEIPNHIGEELFDISIIVGNLMDNAIR
                     GTAASDERRLSFQMYYGKGIMNIQIENSIKDTPKVRNGIYLTTKSRKEGHGIGLQNVK
                     LVVEKYHGQMEICHTEKSFQVKILLYMKLDEK"
     gene            complement(27015..27728)
                     /locus_tag="IXT21_000197"
     CDS             complement(27015..27728)
                     /locus_tag="IXT21_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4867634.1"
                     /translation="MLELAICDDDIILCNWLEQKLLHYGKANDCQISIEIYYSAEQLL
                     NRLEEESYDMLFLDICLEHENGIDIGTEIRKKNYGKELRITYISTYQEYAMKLFRIHP
                     FDFLVKPIREEELFRVIGELRELLKERKKIFEYTDTKGIHKISYDKILYFYSLGKRVH
                     IVTSEKEYEYRGKLKDVADVTEEYFLLIYKSYLINMEHVEHYEYDNVVMKDKTRLSIS
                     KANRKKVRERLLQTEKKRL"
     gene            complement(28132..28692)
                     /locus_tag="IXT21_000198"
     CDS             complement(28132..28692)
                     /locus_tag="IXT21_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867635.1"
                     /translation="MHIKSIVSDEDREELERMLSLGFIIPNQLFKTRQKSRMIIYIFT
                     PVEFLLSINCYLSESYLMFGIGIGTCVACVAWIIWYQMIGKNFEKNVHEFVWGRSGYE
                     TVDIDENAIKIDEDQIYEFKNIDRMFLYKNFFFFITNEKKIFIFKTTDMESDELIKII
                     KTTGILFEKKEKPFNIYKYCKKGKSF"
     gene            complement(28773..31034)
                     /locus_tag="IXT21_000199"
     CDS             complement(28773..31034)
                     /locus_tag="IXT21_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ4867636.1"
                     /translation="MSDQNVKAAQKYLNAMFGGHKDWVKLDEDGKTGTAVMQGIIRAF
                     QIQNGISTITGTVGPLTINTMKKLAIITKMDPNDTPQVNVCLIQCALFCKGYAAGGIT
                     GIYYTSGVNAVKKMQENAGLEVTGKIDWKVWSGLLSLNWFTKVSGGDSNIVLIQQQLN
                     SDWSDVIGVGPCDGIASRQTILSLVGALQAAEGVTTELITDLNSVNFGDATTNAFPGT
                     LQNGQNSTKYVPFNKIAQYGLYFNGYNPGRFDGVFDSTTESKVSEFQEFYGLTGIGLV
                     TKGKVNVSTMKSLLTSKGDTNRAAKACDCATVLNKQQALDIKNAGYTHVGRYLTGSVG
                     KEHTPKYLTSTEVKNIENAGLSVFPIYQDGGYELNYFKDPSQGSVDAQTAILAAERIG
                     IPSGTTIYFAVDFDCYSYQIDTFIIPYFEQIHMIFFSSTNDKNYKVGIYAPRYVCTKV
                     YEAGLASKSFVADMSTGFSCNLGYSMPKNWAFDQFCELNSFSSSPSFPLDKDAYSGRD
                     TGFKKFDAVSTKTDEEIAQENLRAKVKIARNQYVYNVMEPLGYLNKIMDVGVEYDKEI
                     SLGTMMSPQGAIDISTKISTSLESSTGKIYNIKVDIGNDGELTQTCKNQIMEISSNLS
                     DTGIEGADNFGNTIEKIALSVKSGNIAFEINNVFANSVEFSIVFSTSDLLPEEEKEWT
                     ISVALIFTMTLNSNSGLEFNVVEFTKEHSNILAGAVILVLAGALVVNAIPSIIALFSA
                     GAGTVFGLLIQAL"
     gene            complement(31205..32896)
                     /locus_tag="IXT21_000200"
     CDS             complement(31205..32896)
                     /locus_tag="IXT21_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ4867637.1"
                     /translation="MKDIKERVRDKNPKIRNPAARLPKELVRSAVLEAKEKPRELHEK
                     SSGQSESPTQYGTEKIESVQYRAASVAGKSIGKTTYQGGKKLAGVTYRKIKERKSRQE
                     EAKAAEEAMEQGAESGKKLIKLKPEQAALAKENGKRQVKAAPRVVKVSGLSQEKIKTQ
                     ASMQKQQMEKSLQAMQKARAAQMARKSAQASAESGKAVLQVTGKGSKLSVQGITAAIQ
                     KGVVALEKMGKWIAAGGSAFLLVFILIVGIIAGAAFSSNSESSESLSEEVLAYTSVIQ
                     QYASQYGIPEYVSAIQAIMMQESGGRGTDPMQCSESPYNTRFPHTPGSITDPDYSIEV
                     GVQTFADCIRQAGCSSPQDLDKLKLAWQGYNYGNGYIGWALQRGGYTEANALQFSQEQ
                     AASHGWSSYGDPQYVPHVMRYYSGGSLFAGLFGNQQIVSVAMGQLGNSGGQKFWSWYG
                     FDSRVEWCACFVSWCADQSGLIASGNVPKFSLCSDGVSWFQGKNKWQSGGTTPTAGMI
                     IFFDWDHDGTSDHVGIVEKCEGGRVYTIEGNSSDQVRQRNYAVDYSSIMGYGVIN"
     gene            complement(32926..33276)
                     /locus_tag="IXT21_000201"
     CDS             complement(32926..33276)
                     /locus_tag="IXT21_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867638.1"
                     /translation="MKKKEEVTITFYAAECGEFHDLGEYTKCRTLEEAYKKYQKYCRT
                     SANMCPAIEFSIHDPESIYSDMEYPLPLSSKDRGDLELVPYYNEHPLVNEAIRQVEQL
                     QKQQEKKKHRDVAR"
     gene            complement(33276..35612)
                     /locus_tag="IXT21_000202"
     CDS             complement(33276..35612)
                     /locus_tag="IXT21_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ4867639.1"
                     /translation="MPLIKTLSQALRMDKERFKVPKSVQQAIPIQRIWPDGIFQQGTK
                     FSKTYRFTDINYYIASKDNKTEMFLDYSELLNSLDSGISAKITINNRRINKEEFEKSI
                     LLPMKEDGLDHYREEYNEMLLSKITGTNNSIYQERYLTVSVHKRSIDDARTYFARIGT
                     DIVTHLAKLSSTAEGLDAESRLQIFRDFFKGDVPQAFPFDLKQFAKKGTSFKDWMCPD
                     SMEFERDHFKIGDRYGRVLYMQDYASYVKDDMISELCDFSRNLILSIDILPVPTDEAV
                     REIQNRLLGVETNVTNWQRRQNANNNFSAIVPYDMELQRKETKEMLDDLTTRDQRMMF
                     GILTMVHLADSKKQLDSDTELLLSIARKHLCQMATLKWQQVDGLNTVLPYGLRKINAL
                     RTLTTESTAVLIPFHTQEILQPGGIYYGQNAVSKNLLVADRKKLMNGNSFRLGVSGSG
                     KSFSAKEEIVHLALSTDDDILILDPESEFTKLVEALGGQVVKVSATSDNHLNAMDMDA
                     AYGNEKNPLIEKSEFILSVFEQLVGAGNLSAKEKSILDRCAADVYRDYIRSGYTGEVP
                     TLKDMYRQLMLQPEEEARGLALSSELFINGSLNTFAQPTNVNKKNRIMDYDIRELGEQ
                     LMPLGMLVTLDSIFNRVIQNWKEGKTTWIFADEFYLLFRYEYSANFFYRLYKRIRKYN
                     GFVTGLTQNVEELLKSDTARLMLANSEFLILLNQASTDREELAALLNISENQLSYITN
                     VSVGSGLIRCSGNIVPFENTFPKNTDLYKLMTTKPGES"
     gene            complement(35599..36039)
                     /locus_tag="IXT21_000203"
     CDS             complement(35599..36039)
                     /locus_tag="IXT21_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ4867640.1"
                     /translation="MEVKMNKEIRDYQESMFMGLDLRQCVCSVLAILTAIGIYFGMRE
                     ITGQEITGWLCVLGATPFAACGFFKYHGMTAEQFLWAVIKSELLYPKKLVFQSEDIHY
                     SCMQETISLGEKNQKDGAEIIKEREQRAEAKQRKKRKGRSDAFD"
     gene            complement(36043..36474)
                     /locus_tag="IXT21_000204"
     CDS             complement(36043..36474)
                     /locus_tag="IXT21_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4313 domain-containing protein"
                     /protein_id="HAQ4867641.1"
                     /translation="MDIKKTLRYNWEFGRETVAIRVSSYRNNGNLYVGLCHKEGREWE
                     DFGDVTINLPYQFLEPNEAFITGDFTKDMLHFIKEHKLGKVLNETGRSGYATYQKVAF
                     DLARLAEFDPEGVAEHCRFAGIEVPKEKPQKTKKQSRGKER"
     gene            complement(36479..37261)
                     /locus_tag="IXT21_000205"
     CDS             complement(36479..37261)
                     /locus_tag="IXT21_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867642.1"
                     /translation="MAELWTLLTENPATFKGGGIWRIMVNINDALTAIAYGLLVLFFA
                     AGIIKTCGSFTDLKKPEHVLKAFIRFALAQGAITYGMELMQALFSIVQGIVTTVMSGS
                     SMAGSVTELPTEIVDKIESVGMLESIPLWIVTLLGSLLITVLSFVMILTVYGRMFKIY
                     MYTAIAPIPISSFAGEPTQSIGKNFIRSYVGVCLEGAIIALACIIFSAYAASPPAVGD
                     TELSAVTIVWNYVGELVFNLLVLVGAIKASDRIVREMMGLGG"
     gene            complement(37409..37726)
                     /locus_tag="IXT21_000206"
     CDS             complement(37409..37726)
                     /locus_tag="IXT21_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HAQ4867643.1"
                     /translation="MITPMPMGRKQKKWMSLYMALVLTVTVGATTAFAAGDPLSVINN
                     LSTFIFSLIRAIGLILLGFGVVQVGLSLKSHDPSQRANGFLTLAGGVIITFAKEILDL
                     IMA"
     gene            complement(37814..39541)
                     /locus_tag="IXT21_000207"
     CDS             complement(37814..39541)
                     /locus_tag="IXT21_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ4867644.1"
                     /translation="MLYLVFLPFIWWAAAITACAITPDKNFIQILETLSEKLEQPFFI
                     TYTPYTFKCILIFTAAYFLGIGIYESQKRNYRRGVEHGSAKWGNVSEICRRYCEKQYT
                     NNLLLTQHFRMGLDGYKHKRNLNVLVVGGSGAGKSRTYAIPNIMQCNCSMVITDPKAE
                     LLRKTGGVLERNGYEVRVFDLINPETSWCYNPFAYVRDDKDVLKLINNLIRNTTPKGA
                     QSSDPFWEKSETALLQALMLYLLHEAPPEEQNFPMIMEMLGSAQVKEDDEDYQSPLDI
                     LFERLEMRDPESIAVKQYAIYKQAAGKTAKSILISVGVRLAAFNLKQIANLTCTDELD
                     LYSIGEKKVALFCCIPDADTSMNYLVGMIYSNLFQTLYYVADRKYGGRLPIPVHCIMD
                     EWPNVALPDDFDKILATMRSRGISCSIIIQNIAQMKALFKDSYESLIGNCDEFLYLGG
                     NEKEGHKYVSELLGKETLDTNTYGQTKGRSGSYSVNYQQTGRELLTPDEIRLLDNRKA
                     ILFIRGERPIMDDKYDLKKHVNFRYTEDGGASPYDYAKTPLAHDDLKIDINRLDDYEL
                     LSTEDILGE"
     gene            complement(39562..39816)
                     /locus_tag="IXT21_000208"
     CDS             complement(39562..39816)
                     /locus_tag="IXT21_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HAQ4867645.1"
                     /translation="MARLREQYPAGTKICLNSMDGEAGMPQGLEGTVQYVDDAGQLGV
                     KWDNGRSLSLIPGVASFHKKEGQGHPEEKPRKKRESDLQR"
     gene            complement(39857..40168)
                     /locus_tag="IXT21_000209"
     CDS             complement(39857..40168)
                     /locus_tag="IXT21_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867646.1"
                     /translation="MTKYYLKMDNIMKNMFVVALNDELQAQSEQDECLEETKALLKKV
                     SEYDLAQKRLQITAGEQRQIRKALNRLRSKYLAAGRYSDGIDKVIIQVAKPNTSRHMF
                     W"
     gene            complement(40252..41244)
                     /locus_tag="IXT21_000210"
     CDS             complement(40252..41244)
                     /locus_tag="IXT21_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867647.1"
                     /translation="MYMDLTDMISGGTEEQQIPEQKLTKEEYAAKKQQEREEVWAEVD
                     SQAQGVFQDGEKLKGFLDFMAECNTQRTPNLLLIYGQHPDFKVVRSYERWREEHRSVK
                     AGEHGITYMISTEYEKDGEMRRGYTIGKGFDISQMSGKPLEERPQRSLTELIGTVLKD
                     QSVRVQIEDDLPDKVQAQYNPRYRTIYIRNGMSEVTTFHALNRELACAALDQHTGTYS
                     RQKVSAQAYCAAYVIAKRYGVDVTGFNLEYIAQRNECGKKDPQELRDFLNDVRQASYS
                     IRNHIERNFGAKEQEYITDEFAIGDPVKIPETKDEKPSEKASGKEKKAKSQPER"
     gene            complement(41244..42050)
                     /locus_tag="IXT21_000211"
     CDS             complement(41244..42050)
                     /locus_tag="IXT21_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ4867648.1"
                     /translation="MNYGAEPADQVVRYSLEGMEYSLKITGNVAKHVAVFIAAALKDQ
                     KKTRGKTRLTRMLKEQRPMKFFTVPGDRLKEFAKEAKARGILYVVIKNKVNPDQTEVM
                     VFADDAAKMNRVLDRMNLDFVKAESGSMVTEAVEKENAPEKAENQMEKETGENVRTET
                     VVLPEGKIEFELDDAEEMFQIGDEAFPEGNFTQAQEGEVKNPSEPFLRNKNIFSGRET
                     SWEEKNEKPSVRKELGDIRKEQEEKRKRPDRRKNARESRKKRKNMKTKGR"
     gene            complement(42053..43342)
                     /locus_tag="IXT21_000212"
     CDS             complement(42053..43342)
                     /locus_tag="IXT21_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ4867649.1"
                     /translation="MAYTSVIPVHRLDRSIEYVKDKEKTTQKADSLEAAIDYAMNRTK
                     TEQAVFEDTIGCTNENAYEDMLATKKRFHKMGGVEGYHLVQSFAEGEVTPELAHLIGQ
                     ELADRLLKGQFEVVITTHLNTRHYHNHIVWNSVSMVDGHKYHSNAKSYYTEIRKISDD
                     LCRKYGLSIIESNQKKSIPYVQWKAEQEGKPTWRTAIRLDIRESVQESYSWSQFLKEM
                     EKRGYTWKLNRKYISLKAPGMERYIRLRSLGKNYSEEEIRGQILQPKVKRVYQKAVQI
                     HPKKKLTGIQALYYSYLYQMGVLPKRPKRSPYAVREDIRKLDRRIEQIEFLMKHDIIT
                     REQLAAYREPLQKQIAELMKERRRLYRNGGSKTGEERLSEINEELKRLRKEVRMTVQI
                     EKHSLEIEARLQEAEEQSQDEKRVEDKERMQKSQEVR"
     gene            complement(43349..43657)
                     /gene="mobC"
                     /locus_tag="IXT21_000213"
     CDS             complement(43349..43657)
                     /gene="mobC"
                     /locus_tag="IXT21_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ4867650.1"
                     /translation="MRKRNHVIPVRLNAREVRELNEQVERSGLSREEFMRSLILGAQV
                     HAKPCEHHPELLRKIAGLCNNANQLAHVANASGMASEQSIQEMLRLTKETWHLVKEEW
                     "
     gene            complement(43636..43812)
                     /locus_tag="IXT21_000214"
     CDS             complement(43636..43812)
                     /locus_tag="IXT21_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ4867651.1"
                     /translation="MLRNGVMIVIALMSGGFIGVIVTCCMVAAKKSDEDLGGMMKEEE
                     KKEGTVMQCENETM"
     gene            complement(43951..44283)
                     /locus_tag="IXT21_000215"
     CDS             complement(43951..44283)
                     /locus_tag="IXT21_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867652.1"
                     /translation="MERMEQALAYVCCSAEESRVKVQKYCRKIYELGYVPICPRFVFS
                     PFLEESDAEDMQGYGRMSHILLRRCRMVVVCGKEVTGTMNTEISMADRLHIICTTLDG
                     LVQIKESK"
     gene            complement(44356..45168)
                     /locus_tag="IXT21_000216"
     CDS             complement(44356..45168)
                     /locus_tag="IXT21_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAQ4867653.1"
                     /translation="MMPMTNGLSLEELMKEGTYPEEYTEGENWKILHGDTLKLVKAFQ
                     PGIFDAVITDPPYASGGTKQNERNRTTNQKYSSMSAANALPDFDGDNKDQRSWTHWMA
                     EWLYDVRKACKKGAPICLFIDWRQYPSITDALQWAGWIWRGTAVWDKGNSRPQKGRFR
                     QQAEYIVWGSNGPMPINRPVSCLPGVFRYGNPQNRIHVTEKPLQLMKDVIQICEPRGL
                     ILDPFVGAGTTVLAAVMTGYRAVGIEVTDAYYKLGSDRVKIALEAEQKEDEK"
     gene            complement(45180..45926)
                     /locus_tag="IXT21_000217"
     CDS             complement(45180..45926)
                     /locus_tag="IXT21_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867654.1"
                     /translation="MEEKGTMHVHLLTELTGNLYSEESEWEEEWSESDEYGMPLDGTE
                     LADYEEVIREELERYGEDDLMQYFDGSESIQEKIQSAVVTIENKDGILYGCTKLELNE
                     FLSQEELQEFTEYITGQYSDGWGEGFEQRDIKVDGGTMNVHFWHPNMEQPKMYEKKTE
                     QIPTKPEKQRPKLQLLGHDGNIFSIMGDASTLLKKNKQTAEAKEMRKRVLESGDYYKA
                     LLIISEYVETELSPPPKENKKTKKKNQPER"
     gene            complement(45933..46910)
                     /locus_tag="IXT21_000218"
     CDS             complement(45933..46910)
                     /locus_tag="IXT21_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ4867655.1"
                     /translation="MPWVTEEEIQAAKNMTAYEYLRTHQAQRLQKTRTRNEWQLTDHD
                     SFKINELSSKWHWKSRDIGGVSALRFLIEVDGMKFTDAVKLLCEESPSFIPTQSAKKE
                     KPPFKLPERYCNCRRIRAYLNHRGISDEVITYCISHEILYESVPYHNAVFVGKDESGK
                     ARYAFLRGIYEKNGKGFKMEQTGSEKKYSFCIPPERETKRVVVYEACIDALAHMTLEE
                     GKRDKYRLSLGGIAAPKENTQIQSERFKKLPDALEEFLKNHPEIKEIEICTDNDFAGR
                     WAKEQMKKHYEGTYQIICNLPEKEGADYADLAKEKKQTNKCRDRPLERR"
     gene            complement(46910..48073)
                     /locus_tag="IXT21_000219"
     CDS             complement(46910..48073)
                     /locus_tag="IXT21_000219"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ4867656.1"
                     /translation="MMRRFYTAESVTEGHPDKVCDQIADAILDECLRYDPSSRVACEV
                     LATRGNVFVAGEITSGYEPPVFDVIRKVLEECGYCTDGIEMDTFVHQQSPDIAKAVSV
                     SKEFRDNIGAKLRDRSRGAGDQGVMVGYACDDTPQLMPMPTVLAHRITRELSACRRSG
                     YIKDIFSDGKAQVTVEYEDGKPVRLESVVVSCQHGAEKSLKKLDAEIREKVLRSALRL
                     LPPDEDTKILINPSGKFVCGGFDADTGLTGRKLMVDTYGSMVPHGGGAFSGKDCSKVD
                     RSAAYMARYIAKNIVAAEFASKCQVSLAYAIGVAEPVMIEVDTFDTGKVCADDCLAAA
                     IPLVFGVTPVQIMESLRLNRPIFRQTAVFGHFGRKEFPWERTDKVLDLQDAIL"
     gene            complement(48159..52535)
                     /locus_tag="IXT21_000220"
     CDS             complement(48159..52535)
                     /locus_tag="IXT21_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867657.1"
                     /translation="MKVRKLKTRIAAFGLAVLMGISTLSSANAFAAEQTGSEQQTEVQ
                     ASEQAVTSQKDTSVTADDITKAVSDDTFAVETSMEGIHYDAEKEDVTLVSIKDENGGE
                     YHSDKAGTYIATYMVVPKDKSDSYTITRKVTLTDTEGQAHSEENGGEKQKSDTESEDD
                     SDSPVQNYTDVEIETSEEDASAQAIKELKEDIEEGNVMVLSAAERATSSGSTVTLTKG
                     RTIYYPSYIGNYLTCLFTVNGKIAYCLQSQKASPPSGSYVAQVLDSNKNLQKVLYYGY
                     GGAGDLTGSYLSGKTEDEKYVYTHIAASYAYAGEAGFTGCNYNDLVNAGVIAYINYLF
                     GQEEPPKGELSLSSTKLNAVRDGNIQKTPNITLSGDHRNYVTLSVPENVTAHNLSKGT
                     SVTNGKIQIYGGDTFYLSADLLLTGSYASGNLYGSVGKTWRTLVLTTGDSKQDIGVFE
                     SETAAPVSFSVQWLNMTRIELMKKDVNTQNPLSGAVYGIYTDKKCENLLMTMSATGTD
                     GKAVSDYFDSALKTVYVKEITAPTGYKLNTEVYKVAVTAGKTMTVTATDERVTGKVKI
                     AKIDKETLAFKAQGDSVLRGAVYGLYAKEDIVHPDGTTGVLYKQDSLIAQGVIGDDGT
                     LEFSELYLGEMYVKEITPPEGYTLDTTKYEVSVTYEGQDVVEVTRDLTVKEQVKKQAF
                     QLIKISEDGEQTETDLVAGAGFKVYLISDLTQVKNGKLKPANGESYTASDFKNYDFSK
                     EQVAVTYENGTAVPVPELITDTKGYAVSPELPYGSYVVVESTTPENLKTIDPFVVNVE
                     NDSREPMQWRVFDDRPFEFLLKIVKKDAQTGNTVLKAGASYKIYDVTNKKYVEQVVQY
                     PKKEKISVFETNEEGYLITPQELKCSTYRIEEVKAPEGFVRQGSEESLYDGTTIISPL
                     EQTTKGTYKENPQSGIVITVSSNTAHQIDPDTGAAIVEVEQKNDEQVGSLLLTKKGEQ
                     LTEVTGDSVLAKVKALVSKVRNAVSGKEETGIYKGFKYEETGVEGAEFEIYAKDTIYS
                     PDGAKDEAGNPVVRYEKDDLVAKLTTDENGTAVINNLPLGTYYLKEVVAGENFVLNTE
                     QKEFTLTAEDDTQAVVYEGVTYKNERQKVFVSVEKKDSVTGEKLEGVIFGLYAAEDIL
                     SNQGEVLVEKDTLLEKKATDAKGTLTFDSDLPHGKYYVKEEVRKAGYLPNEEVWNVDA
                     TYENQNLAKIELNKEVENQPTETRITKTDATTGNELEGAKLQVIDKDGNVVEEWVSSK
                     EEHVIYGLPEGSYTLHEELAPYEDGYVSASDVMFEVKEDGSVTKVEMKDEYSKVEISK
                     TDLTTGKELEGAKLQIIRKDGTVLEEWITDGKPHSVEKLPVNEELTLREITAPDGYEI
                     AEDVTFTLKDTMEVQKVEMKDARTPEKTTEKTNAPKTGDNQKIWAFVLLALASAGTAT
                     GVTVYRRKKSKMTDNKKETEEK"
     gene            complement(52609..53091)
                     /locus_tag="IXT21_000221"
     CDS             complement(52609..53091)
                     /locus_tag="IXT21_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAQ4867658.1"
                     /translation="MKDRYYFAYGSNMNLEQMKYRCPAAEVVENVRLENYRLAFRGRA
                     PGNGVATVLPEKGSYVEGVLWKITEACEKSLDFYEGFPSFYGKESIRVKNQDGVEKEV
                     FVYMMNAPHKDVPAKPSKFYLDGILEGCKDNQIPTESVMEAVKRTRQEVKKEKNRYAR
                     "
     gene            complement(53104..54060)
                     /locus_tag="IXT21_000222"
     CDS             complement(53104..54060)
                     /locus_tag="IXT21_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase enzyme"
                     /protein_id="HAQ4867659.1"
                     /translation="MIGIKDQYFGTEIEMTGITRQRAAEVVAEMFGTEAYYDGTTYGV
                     WSVIDLEGKKWKFMSDGSIYTQRKVYGRIVDAGGEYSTEMVSPKLSYDEMGKLQEVVR
                     CLRQHGGFVNESCGQHVHVDASNHTPQSLKNALTIMYAKEDILFKALKVQERRANSYC
                     QRVRPEVLEKIRKIPNKSITMDRVRNVWYGGRDGSHTHYDHTRYYALNLHAVFSKGTL
                     EWRCFESTLHAGKVRANITLALAISAQAINQRSTQMKKTLISENPAFTFRTFLLRLGL
                     IGDEYKNVRKHLLANLDGDLAWRYDKSTYECLKKNQRTEGVR"
     gene            complement(54063..55358)
                     /gene="dcm"
                     /locus_tag="IXT21_000223"
     CDS             complement(54063..55358)
                     /gene="dcm"
                     /locus_tag="IXT21_000223"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ4867660.1"
                     /translation="MIAATIQFFDLFSGIGGFREGLRRAGNFVCVGHCEVDTYADKNY
                     RLLFDTEGEWYCNDARTIEPERMPDFDLLCAGFPCQAFSIAGKREGFADARGTLFFEI
                     ARILEAKRPSYFILENVPGLLSHDKGRTFCTILSTLSELGYHVEWKVLNSKDFGVPQA
                     RKRVYIVGYFDFRCAGKVLPEPETNGAALVQVRAGSQGKRVYSPKGLSCTLTSQAGGM
                     GGKTGLYDVGVPIKENTKLGYKLAREGDSIDLGYANLNSRRGRVGHQIAHTLTTGVQQ
                     GTLHFVDLSPPPVVTEECRSLNTRQCGVHKYKGECSGVLAEDGARAVLTPAKENVRQN
                     GRRMKEPEEPMFTITATDRHGILYHGRIRRLVPRECLRLQGYYDWQIDKIIDSTSDAQ
                     LYKQAGNGVTVNVIEAIGRLLQKADSELNTQKVSEKGIH"
     gene            complement(55429..55980)
                     /locus_tag="IXT21_000224"
     CDS             complement(55429..55980)
                     /locus_tag="IXT21_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867661.1"
                     /translation="MAEMKNICGKIPMDLHEKVREEIELTESSTQKFLQQVIEEHFNR
                     VEGKGEISMGAVRTVAVQVSEELFQRLKVVIGKKHMKQKDFLIQIIEEALEKVEVEWQ
                     SKEQPEESEQTTGELGTEETQPEERTEPERESEPEEEAEPDGESEPKEENKPEGESEP
                     EEETEPEGETDPEESKAEEPTEW"
     gene            complement(56135..56425)
                     /locus_tag="IXT21_000225"
     CDS             complement(56135..56425)
                     /locus_tag="IXT21_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation centromere-binding protein
                     ParR"
                     /protein_id="HAQ4867662.1"
                     /translation="MTRPVYSFRPNLKNPEHQRAWEKFQSVPEGQKSQYLVQAILEKE
                     DRKWMEEVIQKTIKESVRELGICGTTEQLKTKPSSEEIPDQMMDFLSQMENL"
     gene            complement(56422..57330)
                     /locus_tag="IXT21_000226"
     CDS             complement(56422..57330)
                     /locus_tag="IXT21_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ4867663.1"
                     /translation="MIIGIDHGYYAIKTRQVSFPSGIIGYDYEPYTMQNVLQYQGKYY
                     VCGTGRQTLVKNKTSNDNYYLLTLAAIAEEIKHRKAERKTEVILAVGLPLSSFGREKQ
                     GFREYLLRKEQPVRFLYESELYEITIKDVKLFPQGYSALALHPEYLKNEPSVLLVDIG
                     GWTVDLMRLDNAVPNAATCRSLELGVIRCIDETAEQVRRNTGLSVTETQIERVLRRES
                     CSMAEEARRIIQENGRKYIERILSAVTESGFDLRAVPTVFMGGGSAILKRHVTAQDAI
                     CRPVFIEDVHANATGYERIVEQMWTR"
     gene            complement(57518..57850)
                     /locus_tag="IXT21_000227"
     CDS             complement(57518..57850)
                     /locus_tag="IXT21_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867664.1"
                     /translation="MEVSIYELLAAARESAKSDYIKGDSILCEKRFHPDTHYMVEIEL
                     LKNDNKLGKKGNYIRKFLTEPEYLPILQKQEKHLIKIKRQAIVQKGNLRYIPPPDRLD
                     RRRERDLL"
     gene            complement(57891..58331)
                     /locus_tag="IXT21_000228"
     CDS             complement(57891..58331)
                     /locus_tag="IXT21_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867665.1"
                     /translation="MFRNKELFLTGRGKSIMDVEMEKRKFLNLCGKRDRALLSDEKRM
                     TLGDMERLTYLTEFFELENYGIALWKEFGDDVKEPFEAMMKMLDEPECIEDRWLDDEA
                     KGEKWLLEFQELALTEEYREWIRNYIDKKYEERGLPYPTGADFD"
     gene            58413..58649
                     /locus_tag="IXT21_000229"
     CDS             58413..58649
                     /locus_tag="IXT21_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4867666.1"
                     /translation="MQKIRPDMDIGKNIQAIRYKNNMTQDQVVAKLNLMGISITKSTY
                     AKLETNRMNIKASELVALAKLFHTDINAFFSGLL"
     gene            complement(58977..59534)
                     /locus_tag="IXT21_000230"
                     /pseudo
     CDS             complement(58977..59534)
                     /locus_tag="IXT21_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
     gene            complement(59531..60409)
                     /locus_tag="IXT21_000231"
     CDS             complement(59531..60409)
                     /locus_tag="IXT21_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ4867667.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(60396..61733)
                     /locus_tag="IXT21_000232"
     CDS             complement(60396..61733)
                     /locus_tag="IXT21_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ4867668.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLRAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(61736..62839)
                     /locus_tag="IXT21_000233"
     CDS             complement(61736..62839)
                     /locus_tag="IXT21_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ4867669.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(62913..64421)
                     /locus_tag="IXT21_000234"
     CDS             complement(62913..64421)
                     /locus_tag="IXT21_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4867670.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(64387..65079)
                     /locus_tag="IXT21_000235"
     CDS             complement(64387..65079)
                     /locus_tag="IXT21_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4867671.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(65101..66075)
                     /locus_tag="IXT21_000236"
     CDS             complement(65101..66075)
                     /locus_tag="IXT21_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4867672.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            66375..67247
                     /locus_tag="IXT21_000237"
     CDS             66375..67247
                     /locus_tag="IXT21_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4867673.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(67705..69903)
                     /locus_tag="IXT21_000238"
     CDS             complement(67705..69903)
                     /locus_tag="IXT21_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4867674.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            70135..71127
                     /locus_tag="IXT21_000239"
     CDS             70135..71127
                     /locus_tag="IXT21_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4867675.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            71187..72608
                     /locus_tag="IXT21_000240"
     CDS             71187..72608
                     /locus_tag="IXT21_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ4867676.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            72601..74100
                     /locus_tag="IXT21_000241"
     CDS             72601..74100
                     /locus_tag="IXT21_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ4867677.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            74139..75533
                     /gene="uxaC"
                     /locus_tag="IXT21_000242"
     CDS             74139..75533
                     /gene="uxaC"
                     /locus_tag="IXT21_000242"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ4867678.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            75692..76375
                     /locus_tag="IXT21_000243"
     CDS             75692..76375
                     /locus_tag="IXT21_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ4867679.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            76377..77213
                     /locus_tag="IXT21_000244"
     CDS             76377..77213
                     /locus_tag="IXT21_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ4867680.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            77224..78978
                     /locus_tag="IXT21_000245"
     CDS             77224..78978
                     /locus_tag="IXT21_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867681.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(79254..79595)
                     /locus_tag="IXT21_000246"
     CDS             complement(79254..79595)
                     /locus_tag="IXT21_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ4867682.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(79663..81834)
                     /locus_tag="IXT21_000247"
     CDS             complement(79663..81834)
                     /locus_tag="IXT21_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ4867683.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            82045..82902
                     /locus_tag="IXT21_000248"
     CDS             82045..82902
                     /locus_tag="IXT21_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ4867684.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(82973..83830)
                     /locus_tag="IXT21_000249"
     CDS             complement(82973..83830)
                     /locus_tag="IXT21_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4867685.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(83815..86517)
                     /locus_tag="IXT21_000250"
     CDS             complement(83815..86517)
                     /locus_tag="IXT21_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ4867686.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(86530..87819)
                     /locus_tag="IXT21_000251"
     CDS             complement(86530..87819)
                     /locus_tag="IXT21_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ4867687.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            87969..89015
                     /locus_tag="IXT21_000252"
     CDS             87969..89015
                     /locus_tag="IXT21_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4867688.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            89017..91170
                     /locus_tag="IXT21_000253"
     CDS             89017..91170
                     /locus_tag="IXT21_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ4867689.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        24133 a        20853 c        15490 g        30835 t
ORIGIN      
        1 tcgcaagtgg ttctttttaa cgacttagtt atcttatcac gtctaaaaac atgtgtcaac
       61 tcttttttgg aattttcata aaatgtttca atgaagcgcg acaacgttgt ttaatatatc
      121 attaaaaaaa ataaaggtca agctaaaata ttatttaagc ttgaccttat tataatttca
      181 acgattgtca aaagtattgt ttgcagctgt gttgccagtt tctatagata aatcacagct
      241 tttgaaacat aagttttgta ctgcttcgat tcgcccgaat tcttttgcag ttatttggca
      301 atgagaaaag cagacatctt ctataggctt ttcaggaaat gctactaaat caaaagctcg
      361 ggaaggaagt gagtaacctg gagaaaggca tgcctgtacg tttttgatgt gtatgtcttt
      421 gactttcgtc atttgctttg attcaggtat ttcttcagcc actgcttgcc aactttctgg
      481 catttgagca aggtcattgc tattttttct attgtattca ttatgccagt ccattatcca
      541 agaaaaagga aattgtacat caatcatatt cagattttct accctgatat tttcgatcac
      601 tccccctctt tcttttgaag atttcattcg aataccacag tctgactgaa aaaaatcgat
      661 atcgtgaata tatacgtcac taactccagc actaacttcg ctaccaatag ttacaccgta
      721 tcctgaacgg attttacatc tagcaacctc gattcttgat gattttcgat tgactcttgc
      781 accattgcca tctctgccag acttaatagc aatacaatca tcaccacagg ccaaatcaca
      841 ttcatagaca cgcacatttg ttgaagaatc aatatcaatc ccatctgtac ttggtccatc
      901 attgttgcgg atgattactt tttctactaa tacatcgtta gaataagtga tttgcaagtt
      961 ccaaaaacct gatttctgaa aaatcaaatc acgaacaaca acatgttctg catgatacag
     1021 taaacaagcg cgtggacgtt taatagcata atccgcaatc caacgaagtc ccttttgatc
     1081 gtattcagct cttgtcccgc ttttttgatc ttttccccaa tacagctccc accaatggtc
     1141 tccttgaccg tcgattattc cttttccttc tataagaata tctttcgccg agagaacatt
     1201 caaaatcgct gctggccatt tcatttctac gccggctact cgtgtatcga tttctggaaa
     1261 tgcttcgata tctgttctcc ccttgaggac tgcaccttct tcaaagataa gatgactctt
     1321 atcttttaaa aaaagggctc cgactaaata aatgcctttt ggaataaata caggtaagtg
     1381 ttctttcgca ccttgatcga ttgctttttg cagtattatt gtatcatctt gttgagtgtc
     1441 gtttgtttta aagttcaaag cattgataat cccttttttt gtccgcaata cgtttctccc
     1501 tatctttcat tgattttttt caattcttca ccaataaata cagtttttga acgattcagt
     1561 gggtaaccta ctagcaaaat gattgcggat acaaataagc agaatgctgg taacaagttt
     1621 gccatcgtat aaatcctttc ttgtactaaa actgattgga tggctccacc tgttgtagac
     1681 gattggtaac caatgagtgc cagcataaat ccgcctaccg ctcctgcaca tgcttgtcct
     1741 acttttctcg caaaagaatt tactccatat actgttccat cttctcttaa gcctgtctta
     1801 tattgatgat aatcaatcac gtcagtaatg aatgcccaga ccatcaaatt gaataaacca
     1861 gatcctaaag tagcgataaa taaaagaatc agatataccc atgcatttgt tatatgaagg
     1921 aaatacatca gcaagtacat acatgaagaa gcaagtaacg caaatacgct tgcttccttt
     1981 ttaccaaata attttgtcag ctttgtagca aatggtgcta aaactactac tgttccgtaa
     2041 gtaaataaaa gagcaacaga cattgcttgt ttattatgga agtaatcatt gaataaataa
     2101 gtcatattcg tcccagctaa aatctgattg atcacaatga aaatgtcaac aatgactaaa
     2161 ataatcagtg ctcgattagc taacaaacct tttaccaact gagaagcaga tactttttcg
     2221 tttttgacaa ctcttactct ttcagtagtc aatttacaag ttaacatata tgcgacaaat
     2281 gctattgcag cacatccgat tgaaatccaa aagaaatgat tccctgaaac gacttgttgt
     2341 ccactcttgt tatgggtata cattaaaatc ggaatgaaga atcctgtagt tgatccgcca
     2401 atcgcagaac caatgcttcg gaacgtggac aacgaggtgc ggtcattagg atttgagcta
     2461 attgctgctg ccattgctcc atacgggata ttgattgttg agagaaaaat cccataaaca
     2521 atgtatgaaa caaaaatata agcaatcttc accgtcattg gaagatgttg gacgaatggc
     2581 aaaaacaaaa tgacagtcac taagcaaaat ggatacttca tttttctgat ccaaggaata
     2641 aatcggccag tttctgttaa ctctgagaca tcacataatc ttccaaccat cacatcagca
     2701 aacgcatcca aaattctcga aatcaagaat aacgtaccta cgattgcccc tgaaacgcca
     2761 agaacattcg tataataaat catcaagaaa ctattgacta aacctaaaat aaaacagtta
     2821 cctagatcac cgaacatgta gcctatctta tcggaaatcc caaattcctt tttttctttt
     2881 tttattgtat ttgctgtaac agcattttgt gaaagcattt gaattcctcc tacctcttct
     2941 agtaaacgtt ttcaaaatta taactctttt tttcttgttt ttatattcac aatgttggct
     3001 ttttattgca ttatcttgac agataaaaaa caaaaaataa tctaaaaaca ttgatgcata
     3061 agattgataa ctttttttac ataacaatat atacaaacaa aaaaattcac taactagtga
     3121 aaataagtta gtgaacctca tttattaaag aattaaatta cagatgatct tttccatcat
     3181 acttgaaaaa ctcaaaatca gcataacttc ctcttttttt atttagatca tgcactgcta
     3241 tcccgataaa atttccagta aaccctcctg agagtataca catgtttttt tgcggaccta
     3301 ctttcttcca ttcttcagtt gatacgtctt tcaaatgaaa ctgtgctgtc tctttcttta
     3361 cagtgacttt caaaaaagtc ttcttttctg tgagtggaat cgtccaatct tctatttgtg
     3421 ctactccgtt ttctgattct atcatacgta ggcattttcc cttgtcctca ttccaagtaa
     3481 cataacaata aatataattg gattcattta agtacagtac cagtccagcc atctgattaa
     3541 aattattagg catgtattca aatgctgtct ccgcatcaaa agtaaaatcc gtttgccgaa
     3601 tagctaacaa atgatgttcg aaggtagatt gaagagattc accggaaatc agacgtaaaa
     3661 aacctggtct acattttgta tcacaccaat cagcatttgg caaaatgcgt ctggcattcc
     3721 agttcttctt tagctctcct tcaaatgtat ccataaactg atgatctttt tcttgttgta
     3781 cctcttcatt tgtctcaatc attatttctt ccatcggagt tgtctggcta tctttaagtc
     3841 ttaaccaatc gtcggacgtc caatacactt cttgaatagc ggtttctctt cccaaaatag
     3901 ccgcaccgtt taatggtctg gtacaaagat atgtcatgta ccatcttcct gacggggttt
     3961 gtactaagct cccatgtcct gtacattgca atgtagaagc aggtttgtca ctagctgtca
     4021 gcataggaaa accaggatca agctcgtaag gaccagtaat attctttgct cggcaaactg
     4081 taactgaatg tcctcttcct gtaccgcctt ctgccgtcat caaataataa tacccgttac
     4141 gaaaatacaa atgtggtgcc tccgttttcg ctaattctgt acctgcaaat attttataga
     4201 tgggaccatc aagcttcttc ttctctgcat catattcttg taatacgata ccgctggatt
     4261 tattcggtgt gtccagacga taatcccata gttcattgac aagccatttt tttcccgttt
     4321 caagatcatg gaacaaagaa ggatcaaagc cgttactatt taaataaatt ggttcggacc
     4381 aaggacctgt gatctcagat gcagtgatca aataattgtg gacatcttta aacggacggc
     4441 ctgcactttt gacatctgtg taaacaagat aaaacaatcc atcagcataa ctgatctgtg
     4501 gcgcccatat acttccattt gtaggatttc cttgcaaaga tacttgagtg gtcaaaatat
     4561 ccgttgtatg tgaccagttg actaaatttt tcgactcata tactcggatt cctggaagcc
     4621 attcaaaaga ggagacaata atgtaatacc gctctttcac tcttaatata ttaggatctg
     4681 gattgaatcc tcttaaaata ggatttttca ctttgatagt ctgcatgatt caggctcctt
     4741 ttctcattct tactcgatat aaactgaatg ccccagctgc gaaataaaaa aatgcaaaag
     4801 gaaacgcaat aaactgagtg ccaatcaaca atatgaatcc tgttataaaa tacaaaccag
     4861 tggcagttgt taagtatttt cctttagcag tcaaaaacaa tgctactgct aaaaaagcaa
     4921 gtgaaataaa caacacaacg gcaaacagac cagtcatgtc ctgtaataat ccaaatgtat
     4981 tttctaaaga caaagttgaa tgatctacag caagcgactg ataacttttt ttaaactctg
     5041 acgcagacat attacttaaa gtaactgcta acccgccaaa gaaaatcaaa gatagactgc
     5101 caccgaccat tcctagcaat agttcccatt ttcttttcat ttctgaaccc cctgcttttc
     5161 taaatgtatc cattcaggtc ttttgatcga tgtttcataa ttgttattac gttcagagaa
     5221 tttgacagtc tgttcatttt tcttgtcatc ccaattatcc caaccagttg ggtgaacatg
     5281 cccacctatc ttacacgctt caaataagac atttgcatac ggacgccacg gtcttcctaa
     5341 atagacgttt ctagtatcag gttctgcagt caaggtacag ctttcaaaac gataccctgt
     5401 tttttgattt ttcggcgtag atgctgctgt gatgtatcca ggatttttac tggctaagct
     5461 atggatatga cactgatgaa aaacggcatc tgccccacca aatagggaac taaaatgtag
     5521 ccagttcccc tcatgcgttg attttactgg gtttaactgt atacaaaact gtgtttactg
     5581 ttactatctt tcattctttt ccaaaatagc tttgaagcat aggccacatt attttcatat
     5641 atgacacaaa gctcagctct acttgtgctt cattaaacca tccaaccata aagctattta
     5701 tctcttcaaa taatactttt gaaagtttct gaataataat tgtgttatcc acttcttcag
     5761 tataatcttt cgcatacatt ttacatatag aacgcactcc aaaataatta tatagcacac
     5821 ctaccgttgt ttttaatgct tcaatatctc ttggaaaatc tgttctattc atcatattca
     5881 ctactaattg atgtcttgag tttaggtaat tcctaactgt actattacct atttcaacat
     5941 caactgtaaa tgaaaaatct tgagtttgta tactatctgc tcctaacatt atcatcaatt
     6001 caacttgtgc tgcatgatat tctgtataac cagacaaagc catatatttc caagaatctt
     6061 gctttgcata catttccgta tctaaaatat gcgtaaattc atgaaacaca atataccttg
     6121 gtatttccga acttttgcat atccttaata tatgttcatc tgttttcgta ttatagtaag
     6181 cttgggcggc tgaagcgaat cctgctgcat cgctttcctc aacattaatt tcaaaaaaat
     6241 ctatcttata ctgtggtaat ttctcaattt ccataaactc aatatattct ttttcatatt
     6301 ctttaattaa atttttaatt tgtatttctg tcatattgtt ttatacctca tatcaaagtt
     6361 agcatttcac ttttagtata ccataaaaca atcccattct cattctatat attttatcct
     6421 caacgatgaa aagatgtgtc tgcacacatc tcttccatct tattattttc aatgttataa
     6481 gttcttaata aaattcaaat cctgtttccg taatgccttc tgtaactttt cgttctttct
     6541 cttcagttcc ttattttctc tgctcagagc tgccaccttc tggttcaaca gttcaatctg
     6601 tttttccatc gccatgttca gaatttctct tcttggatct atcattccgg cctgttgctc
     6661 tactgcttgg ttcaatgtat cccggacggt tggattttta taaaagaatc ctctcgacaa
     6721 tccagttttc ttcatcagct ttggaacgct gatacgttct ttatttgcca gcatatcttg
     6781 tattgcttgc aatgccagtt caattttctc tttacttttt ctctgatttt cctcaatcat
     6841 cttgtcgtat ttgctcatat tcttcacccc atcttgccag actcatatat atgatgtcac
     6901 tcatcctctg tcttacttct ctggtttcgt ctgccattct ttgattgctc attttccggt
     6961 aatgctgtac attattcagt cttttatgcc ccaatagtct cgctatcgtc cagtcatcca
     7021 gatgcatttc tgtaagtttg acgccatagt agtgccggaa catgtgcgtt ccaaacccga
     7081 ataattcgcc atgatcatct ttcaactgct tttcctgtat catggacatg acttttgttt
     7141 ttatagccat atactgcatc gggcggtcag gttctttttc attaacaaat atgtattctg
     7201 tatcaccgaa cttttcctgt gtatattcaa tagaactctg taacagtttt gcaagttctt
     7261 tactgatcgg ttttttatat cttttagttt taacttgata tatttcaatc atatcttggc
     7321 cattttctct cgtcagacaa tctggtctca gtgtaagggt atctgatatc ctagttccaa
     7381 gcatctgatg tatcagaaga catcttgcaa tctgtacatc catctgtgta atttccgcat
     7441 tcagtcgttt catttcatca tcagaataca ctctaaattc tgcatttact tccggtggaa
     7501 tatccgtatt aataaataaa tgctccaatc tttcatagct gtaaattttt ccgattgtct
     7561 ctaactgtcg tcttaatgaa attacataag aactattcgc actatgggaa gtatcttttg
     7621 ttgacagatg tatcaaaaat tcttccaaca cttctctgtc tatttcactg cagcttttta
     7681 ttttggaata ctctttttgg agatattctg agaatatcct cattattgtc aattctccct
     7741 gtacagttcc tattgcttca tactgcagat tcatataaac agctttctta cattcttccc
     7801 tgatatctgg ctgatatatt tttctgaaat ccagtgtttt tacattataa attggattgg
     7861 atctgatttt aatatccaga ttttttagtt cccaaacatc tttttctatc tcatcccgta
     7921 aatcttctgg ccctaaaaat ttcagtacgt ttctgaaata taatatcgtc actgcttcgc
     7981 catagcttac tgtaccatac acactcttct tttcttttac aatagttatt ccctgttcta
     8041 acatccagcc ttttaaaagt gatatccatt tttctggatt tctatctcca aggcttttga
     8101 ttctccgatt tattctgctg ttcaaaaact ttgcaatatg attataagct gttcgatcct
     8161 gaatcaatgc tctaaacgtt ccgtttttgc agcgatacat aatataacct cgaaactctt
     8221 cctttaattc ttgcgttgga agcaacgtta aatcaaattt tccattatcc ttacgtgtcc
     8281 ctgtttcttg taaattgggg aaatcgctga agtatataat atcattttcc atctttttcc
     8341 tctttcttta gccatgatgc taaactactt ccttgcattt caaagaactc atcatttgct
     8401 tttgcaatcc tttgcggcat ataatcaata tactgtcttg tcatctcttc ataagtatgt
     8461 ccaagataat ccctgatact ttgcagtgat acatgactgt cataaaacat agttgctact
     8521 gtatgtcgat aatcgtgtga ttgaaataaa tattctccat ttgcaatctt attttcccgg
     8581 cacgcttcta tcatctgagt tcggaatgta ccatatagac aagcaccacc tctgctgttt
     8641 ttaaaaatga attcttcagg ctgaatttca tgttttttca agtatacttt cataatctga
     8701 taaagtgcct gtgggattgg aatcctttta taattcttca ttttgacctg atacacctga
     8761 atccaataat cctcgccctg ctgataataa gcatttcctt tcaatgtaca cacttcactc
     8821 gcccgtaatc ctagacacca cagatgtagg aacatacatc gtaatcgctc tggaaaaaga
     8881 tataattttt tcaaaatttc catatatact gtttcctcaa cgctacgatc atgatgtact
     8941 attacttctt tttgttggaa atattcaatt cggaatggca ttcttgtaat atactgttta
     9001 acttccatga atttataaaa atgaccgata ccgaaaattc tttcattaaa tccctttgcc
     9061 tgtattcccc gttctcgcaa gccgtctgca tactttttta cttctgcgac agttgcatga
     9121 atcgccagta tcttttcttg ttccagcagt tcaataaaat ttctaataca tacaaatctt
     9181 cgtacaatcg tgctgactgc ctgtccagta attcccagtt catatttcat gtatgcctgt
     9241 aaaatttctc gattctgtat ttccttgact tccataaaag aaatgctaat taaggattta
     9301 ctttcgtcta tccgatgttt tccaagatgt aatcgttcca gataccatac atttgcttcc
     9361 cattggatct gttcctgctg cataaacgca tctcttctgc attcacctaa aatacttcgt
     9421 tgactcgttt cgggaaaatt ctttaaaata gtttccactt gttccatttc cagcaatcca
     9481 atatccgtaa tatttaactg aacacaagaa tcataaagaa gttttaagga ttttaacctg
     9541 acatttctat aagccttaga cattgaagta ttggcaatga tacgactcaa cgtataaaaa
     9601 atttgtttct ttaaaacttc tgaacaattt tgtgtaaaat cccacaccaa taggtttgtt
     9661 ttataagaag tttcaaaggt ctcagctatt tccttttccg gataatactt cagaaaataa
     9721 attttatttt gatatttcca ttcatatatc cgctttccat ttagcgtctg catttgttga
     9781 tagatatgtt gttgttttac cgtatcatat attttcaaat agaaagatac aatttgtgat
     9841 cgtatctgtc ctcttaaata ttgttcatac gaatttcgca aaggataatc catttcttcc
     9901 agatgccaga tttttctatc tgccaagaac tttttcaatt tgttaattct tgaatcgctt
     9961 tttattccaa gtgcatcaat ttctgaatat agttcctgct ttttcttctc aatatctact
    10021 tcagattctg ttatgtgttc tgcctgttgc aataaatata taggtatttc tcacacctcc
    10081 ctactccagc agacgttccg ccccataaat agcagtgtgt tgtgcataaa actcattact
    10141 tgcttctatc agttcatcat ccagccagtc cagatacttt atagtcgtat ccaaatgttt
    10201 atgccccaat gcctgactga tcatttccag ttcccatcct gcctgctttc gcatatttgc
    10261 aaaatagtga cgtaacatgt ggggtgtgat tttaactccg gttttctttt ccattcggtc
    10321 caacatatca tatacactgt ccactcttaa tggctttcca atgttctcac ccatgatatt
    10381 cacaaacagg taatcctgat gttgcaggat tttccgatat tttgccagat aatccatgag
    10441 aaattcaaac gtatcattac ttatcttggc acttctgtat tccgcatttt tggctctcgc
    10501 ttcattttca ttatcttccc ggaagcatac ccttatcatg tgattcctat aatcaatatc
    10561 tttgacatat tttactccca gtagttcccc aattctgtat ccggtttctg ctaacataag
    10621 taacagaagc tgatctcttc ggttcgtaca ggcctgtaaa attgtaataa tctgattttc
    10681 ttttgctgtt ctcccacgat gttcctcttc cttcagatag gctttaaaag aatgattccg
    10741 cagtaccttt tttactccca cctgattaac cgctataatc tggttatacg ataaggtttt
    10801 gagacttccg tactgttcat attctgcctc tataaataaa tagaacctga acacatcttt
    10861 taagtatgcg ttgcaggttc cttcattcgg acacttcttt tctccagccg tttgctctgt
    10921 atggtttccg gctttaagcc agtataaaaa ttccacaaag tgttctgtct gtttttcata
    10981 atctaactga tagacttctg tgagttccat ttctttctct gccatatatt tcatataata
    11041 gcagattgaa aatgctgtgc gtttcactgt atttggtgaa cgtttacatt taatcttatg
    11101 cattagatat ttacttggca gttcttcaat actcattgtt tccagatttc tgatgaaaaa
    11161 atatttttct gttccatctt ttatcgtttc caccttgtat atcataagct gcctcctttc
    11221 ctctaacgct atacacagta tacccacttc atgccgaaat agctattcat caataccaac
    11281 aatcccaccg aaaacgccct tgaaatacca ccaaactttt cgtgcaaaat ctgctttttc
    11341 tgtagcagaa ccacgaacgg ttacaggtag tattccacgc ctttccggtt tcgaataaag
    11401 tgctggacat ccttgatatc ttttgccatc ttgcagctcg gaagcgcaca cattacttcc
    11461 tcatgatacc tgccaccttc tcctgcacgc tcgtctaaaa tggacacgac acaagtatct
    11521 gtctcggtgc ggagagcacg tccgaatccc tgtcttagtt ttttctgcat gtctggaaca
    11581 atcactgcct gaatataatc tttcaggctg gcatattcct tcttctgtgc ttcactgatg
    11641 ggatcaggaa cagcaaaggg cagttttact ataatcagcg aagataccat atctccaggg
    11701 aagtctactc cttcccaaca ggagccggct gcaaaaagga cagcattgtc catctgttta
    11761 aatcttgtga tctcttccgg tgaatgtctc cacacctcta tcatcgggaa cggaatttca
    11821 tcccttaaca tctggtaaac gtttcccatc aaatgataag atgtaaacag cactagcgta
    11881 tgcccgtaag tcgaacagat cagtgaatgg atatggcctg ctatcatctc tgcctcttcc
    11941 tgactttctc ttctgcatgt cttcaaattc tttggcagat ataacagaca gttcttctga
    12001 tattcaaacg gggagtctgc cacatattcc cggactctcc gcactctctg taatcctgtc
    12061 attttccgaa tgtgggaaaa tccctgtccg gtttttaaag ttccgcttgt cagaatcgct
    12121 cccattcctc tgctccataa catctggtcc agatactgtg gaatccgtct gctggatgca
    12181 cacaatataa tccggtggtc atggtcctgc ttcagatgca gcacatactt cttatcctga
    12241 tggaaaaagc attccagcac acttttggtt tcttccagct tgtttctgat ccactttgga
    12301 atcttctttt ctaatttttc tatgatctta ttcatggtct gtatcccctc atataaatac
    12361 attgcacact cttctgtcat gtggaattct tcttttatcc catatctggt tctgtggttt
    12421 tctcctatga tgtccagcac catccggatc gtcccggaca gttttcttat ctccgggcct
    12481 tggtactcat tcccaagata aaagcagatt tctcggatat cgtcataaca gaggctcttc
    12541 ccaaacatct gctttgccgc atccggcagc ttatgtgctt catcgacaat caatgctcgg
    12601 tagtctttta ataacgggcg gtaatcctgc agcctgtgat aaccatcagc cagcagataa
    12661 ttgtgattac agatctgaat gaacatttca tgatctctgg aacgctccag atactgctga
    12721 tacctgcatg agcctctttt cgggcagtct cccgaacaga acttcggtac actcaccatc
    12781 cttctgtcaa acccacttaa gttggacact tcgtccatat cataatgttc tttcaacgac
    12841 agcagagctt ctttctgcaa cgcatttttg tttttttctt cgattgcaac gattcggtgc
    12901 tccaacctct catcgcagac aaaatgctct ttcccttttc gtatcacagc tttaataggg
    12961 gcctgaatcg tcccgttctc ctgaagaatc cgggagagaa aagggatata ttctgtaaga
    13021 attgctttct ggagtgcaat gctggaagtg gatatcacga ccggacgctg ctcataaggg
    13081 gaacatcctt ctgcaaggag ggaatatttt ctcatcagaa cacaggccac cagataggca
    13141 tacgtcttgc cgataccgac acccgcgtca cacagtgcta tgtttcttcc cagcaggtta
    13201 tccagcattt tgtggcataa cataatctgt tcttccctta ctgccattcc atgttccggg
    13261 aacagcactc ggaaaatagt atcaatttct cgatgcgctc tattgagata ttctcttgtg
    13321 taatacataa tttgttacct gatatgcgtg ctgatatcac atcaatacgg ctgtaaagga
    13381 tttctcccta cagccgtatg tatctgatat caacgacaca ttcttccttg atttcttata
    13441 cttttgattt ttggcgttgt atttgtcctc gactccccca ttgccttacg ggaacattac
    13501 aggcggatgc ttggcattgc accgctcacg gattttctaa aagtcttgct gattacaacc
    13561 caaagtccat cctctgctca ctggcagttc ctatgtatca ttatcccctc gtaagtgatc
    13621 ctcgcaaaag accgccacgg tcctgtttca gttaaagatg attcgctcga aacgaaaacg
    13681 ccacaaaaga actctgcctc tcactttctg gtctaatttc ttccatgccc ttttcatctg
    13741 tcctcgcgtc ttcctgccac gcttctactt acgggaacgt acctgtaatg ctggtatgag
    13801 gttgtcaaag tacactggca aaatttacct gcctattaaa tagccgacga aaaacgccat
    13861 aattattcaa tttttgataa attttttaat ttttttctca aacggtttct tttggtataa
    13921 atcgtctgtt ctgccattcc cataatctta gcaacttctc ttccagaata tccccatgta
    13981 ctgagtaaga ggatttccag tgtctgatgc tccgtttctt tcaatagttc atagattcgc
    14041 ttatcaccta tgtgatccag aagctccttt acatcccttg caaacagctc gctgtccttt
    14101 tcttttatgg aatccatatc ctgcatcgtt tcatccagat gttcccggta tctgcgctct
    14161 tccagaaagt cttccctgtc catctggcgc agcttttgga tcttctcttc ttccattccc
    14221 agttcacgca atttcttttc ttcctgttct ttccaataat tccactttcg ttcttcactt
    14281 gtcttgttat aagccattta tctttcctcc gatctgattt ttgatataga aatcaaatcg
    14341 gagcatggcg gtgaccggat aggtacgtta taatgttcga aaaatgtcga agaaaaaaat
    14401 gctgcaacct cttcgattac agcacttttt tccatcttac gtgaatattt cttgttggtt
    14461 cataggagat tccttctttt tctcataatg aataaaagac tcttgtcatc cattatttat
    14521 tataattttt ctactattac aatagctttt ttaacaagtt ttcttgtctt ggagtttaaa
    14581 agaaagctac cagttttttt cctctgatag ctttccattc attcttattc tgttgggaac
    14641 atcgctgctt ccaacttatt catctctgta tccaaaagac catattttcc tgagttatct
    14701 ttttccacgg atacgtttac agtaacagct tttccatatc tgttcttttc aagactcttc
    14761 tggacacttt ctgttaatac agctgcaaag acttccggat catttccatc caatccctgc
    14821 tttatttttt cattgcttac ctctgtagag ctttgttgca atgtctgaaa tacattggat
    14881 ggttcaactt gtacacttac tgtatagact ccatcctctt ctttcacatc ttgtactttg
    14941 tattttgcca gtttctttac ttctgtcagt ttttccgcat aatcagcaat tcctttatcc
    15001 gtaatgttat cggaatcatc aaattgctgt tggatactct cattaaaatc ctcttcgatt
    15061 tctttcttgg catctttttc cgagatttct aacagattcg catagtcctt atattctcca
    15121 tgatagtgtg catcaagaga actttggacg taggatttgg catcaaagtc tggttgtctg
    15181 gagcaagctg ataacaatat acatactata attcctatac ttatgcacaa aacacaattc
    15241 ttcttttgca tattctctcc ttcacgcttt atttatcttc aatttttatc gaaaataaca
    15301 tttttaaata ctgttctatt gactcctttt gaacttttct ttcaatatta tatggaatgc
    15361 acataaaaaa ctccacataa ccattatcaa taaaactcag caatacttct ccattttctg
    15421 ttaaaaaatg tacatatttt ttattacatt tactttcaaa tttaatattc gacatttgct
    15481 tgtatatttt tttgtaatga ttttttatgc ttacttcatc tgtacaatca gttccatcac
    15541 ctatcaaaaa agaaatatac aacttatcat aatcttcatt tctatatgtc atataagaat
    15601 ctgtatgacc atcaccaata tttttataat gctcatttgg aatcgaataa ctaattattt
    15661 tcccctctaa tgtttcaaat ctttctgttt catcaattct aatttcacat ggttcaaaca
    15721 ttttttttat aaacaagccc aattcataat ccgacatttc tataaaatta atataatctt
    15781 tttcttccgt ttttcgcgaa tatccccctc tattattccg ttcccatctt ctaatttcta
    15841 attcatctcc attttgtttc aaatcaattc tttgataatt cttggaaaat gttggaaaac
    15901 ttttatattt tgtttgtttc caaaaattgt ttaattgaga ttcgtctact tttggggaac
    15961 tttctgcaat ttttattcct tttctaattg cacagcctat cttatctatt gtttcttcag
    16021 ccataatttc aattgtaggt tcgatagaaa tataacatcc cataaacctt tcaatggcat
    16081 taggcacaat taagaaacca tatttttgat tataatatac agaaatcata tccgttttct
    16141 cctctctatt acttaagggg aataaaatat tccccttaag ctatttactt tgctattatt
    16201 ttcctactgt aataatgagt ttaacacctt tatcaatacc atgttttgta gcattattaa
    16261 ctgcatttat ctgagcttcg cttaatgtta catttggaac aactacttgt aattgtctac
    16321 ccactatttc ttccgcatta acaaatttac cattaaatgt ttctcctata aatcctgaaa
    16381 ctttattaat gtccttgaca attacctttt ccaatttatt accattttga taagtctttg
    16441 cacctaagtc tcggctttta acactagtca caacaccatt ttctacttta tctatggtcg
    16501 ggaaattgtg tcccaagtta tttcccatag ctttgtcaat aatatctcct cgctctaatg
    16561 gtggtttttc ccaagtgcct tctggcaatt ttgtttcggt tacttctgca actaattttt
    16621 taaacgattt atcaatccca tcattatggg tacttcctgc actccttact acattcaaaa
    16681 gtttcccagc cccataacta aggccaccca taacgccacc tgtcactgct cctgcaatac
    16741 caccgactaa cgtatctttt gctacttgtt tcagactatt tccagacaaa cctgaattca
    16801 ctgcactata tgaagctcca ccaatagctc caataactgc tcctgaaacc atcgccgttc
    16861 ctaagccacc cattgctggc aatgctgcca gaactgctcc tgcagccaaa ccgcctgttg
    16921 cagcaactac aagaccagaa actgtaaccg cagttatacc aatggctact tttttcaccg
    16981 cattccagtc tacttttcca agcttatctg taatacgatt tgttgtcgta cacatatgtc
    17041 gtaccacttc tgagcggatt tctgccgttc tttctgaaac ataggatttg aaagatgtat
    17101 aagtttttcc aattttctgt accccattgc ttacagaacg aataaactga cttcctctgg
    17161 atgtgatctt ttgatatgta ctgctactgg ttacacgatt tttcacagat tgatatgtct
    17221 gttttgcatg tttagctgca tttttggcag tttttgctac ccccttcact gtgttaacaa
    17281 tagtttttgc tgctttctta gctgtattta ctactgtttt gactaccttt ttcgcagtat
    17341 taacgatttt cttgccgaca ctcttgactg ctttcgctgc ttttttagct gtactcttaa
    17401 tcctgctcca caatgaatgt cctgtcggat cactgtaatt cagcggatta ttggtcgtat
    17461 aatcgtagcg gtttctggtc aatggatttt ctgtcgttcc aaggtcacta tcttcagttg
    17521 taaaggttcc gttctctgcg ttatagtatc ttgcccgcag atactgaagt cctgttttta
    17581 catttgtgga ctccccattg taaccgtagt aattgacacc gtctgctgtt cctgatgtaa
    17641 ggttaccgta tgaatcatac tgataactgt tcgtcagatt agctgtttcg gtcaggattc
    17701 cggtcacgct gtttcttccg tcatataaat aataactgct ttcttccgat ttatcaacgc
    17761 ttacccgttc tccgattcca ctttctccat aagtataagc ctgacgtact ttctcgtctg
    17821 ctccatactc tgcaaggact tctgtattct cgcgattgat atcgttgata tactctgtca
    17881 gtgtgtatcc tttggcattg gaaccgcctt ttaccagaga ggcaagctgc tctttcgggc
    17941 tgtttccatc ttcggtacgc tggttcgccg ggatcaggac ttcatcgcca tagcagtctt
    18001 cccatttata ggtgttatca agttcgaata cccggttgtt atctccatcg tacatggctg
    18061 ccatcaggac tgttccaccc tgttttacta ctgccaaacg attctctgct gtgtattcat
    18121 aagtaaccgg atctgcactg tttgtcttat cacattcctg gatcagattg ccatctttat
    18181 catagctgta ggttgttccc ggatcgcccc agatctttgc attggtgcgt ttgatgagct
    18241 ggttggaatc gttgtactta tagttatatt ttgctttgct gacaccatcc tcgatcttct
    18301 cgtaagaggt ccggtttcca atcttgtcat aagtataatt atggactgtc tttttaccgc
    18361 cctctgcttt ttctgtacag cgtgtcagtt cccagttgtc atcgtattca taggtacgtg
    18421 tcgtctggat ctctttttcc tgatgctcac agtctgcttt gtctgtctct ttctgtgtat
    18481 cgccccagtt gtaccagtct tcccagcttg gcgtctttct tgttccagct tccagttccg
    18541 tagctgtttc tccaactaca tatccctgat cattgtactt gtactcataa gtactgatta
    18601 ctttgccgca ggaaccacag gtattcacaa tctttgtgat atgatcttct gcatcgtaag
    18661 atacttccgt ctttgttcca ttacttctag taacttctgt cacacggttc agtgcatcat
    18721 gcttgtaagt ggttactttt ctattacggt ctgtcagttt taccagatta tcatttaagt
    18781 cgtattcata gctgatcttt gtcccatccg ggtatacgat tgcctgcaga ttatctgcgt
    18841 catcataaac gtagctgaca tcctttttcg aaccgcttgt cactttggtg attcttccaa
    18901 gtgcatcgta ttcgtagctg ctcttgcctg tctgatcctt catggatacc cgttccccgg
    18961 cactgttata tgcgtaggtc acgtcctttt cggatgtctc gccttttgcg tcctgataag
    19021 atttctccaa caggtcattc agtttgtcgt aatcatacgt ggttttcttg ccgcttgcct
    19081 gtgtatgtcc ggtcagtctg cctttttcat catagtcgaa tttctccgtt cttcctgccg
    19141 gatctttgat ggatgtcagg ttgccttcta agtcataagt ataattcgtt gtcttatttg
    19201 ctgcattggt gaaggtcacc aggttatcca tgttgtcgta accataagtt gttacaccgc
    19261 caagggcatc agttactttg gtcaggttgt cattcttgtc atattccaga tggctcttta
    19321 atccggtctt atctgtaaca acctgaatat tcccatgtgc atcgtaatca aatcccgttg
    19381 tgaatccacg cttgtctgta atagaagtta ccctgctgtc cttatcatag gtataggatt
    19441 ccttctgccc cagtgcatcg gtctcttctg ttacacggtt gtctttatcg tagactgtct
    19501 ttgcaacata gcccatcgga tctgttacag atgtaacatt ataattctta tcatatccat
    19561 agctggtctt tcttccaccc ggtttggtga tctcagtaat atttcctgcc gggtcgtaag
    19621 tgtaagcagt tatcttacca agcggatcag ctgcagaagt catctgacct gccaggtcat
    19681 aactgtaggt attgctccgt ccaagcggat ctgtctcctt caccagattg ccatacttat
    19741 cataagaaaa gctggttgtt ccatccagtg cattggtaga agacttcatg ttatggagct
    19801 tatcgtaagt ataagtagtg gtactcttta agctgtcgct ttcttttacc acgttgtcca
    19861 ctttatcata agagaaattt ttagtataac ccagtggact ggtcatactc ttcagacgtc
    19921 cggctttatc ataactatag gtatactctc caccattcgc ctgtgtcatc tttgtgacat
    19981 tgtcattcag gtcaacactg tactgtgtct catttccctt tgcatccgtt acagaaatga
    20041 cattgccatg cttgtcgtaa tcatatttgg taactgctcc ctttggagaa atctgttccg
    20101 tcatgttact caggctgtca tatttgtatt cggtaatgag tcccatcgga tcttttgatt
    20161 tggtaagatt tccaatggca tcataacggt attcatttgt ttcttttaac ggagaagtct
    20221 caccgatctt gcggtccagt tcatcatact ggtaggttgt cgtatttcca tttccgtctg
    20281 tctgggatac gagattgtca tttttatcgt aagcatactt ggtaaccgta ccgttttcat
    20341 cgacagattc cgtcacatta tcatttccgt catacttaaa ggtgctggat gctcccagtg
    20401 gatttgtctg agaaataacc cggtctttct tatcgtaagc gtatttatat gtggcttcct
    20461 cttcttctgt catggataac tggttgccga ctttatcgta ggtaaaggtt gttttatgac
    20521 cttccgcatc cgttaccgct gtaatgcggt ccataacatc atattcataa gatgtgatac
    20581 ttccgtctgc ttcagtaagt ttcaggagtc tgccgtactg atcgtacttg tatgttgctg
    20641 tcctgccaag ttcatcggtc tgtgtcagta catttccggc accatcataa gtataatcca
    20701 tactgtttcc accattgtcg gaggaatgga tcaggttgcc tgctccgtca tattgatagg
    20761 taattacgag tccctgtgca tccgtacatt ttacaagctg tccgatggca tcgtactcca
    20821 tcagcacctt gtcccctgtc ggcagtatta tggatgtgtt atttccctct ttatcataca
    20881 cataactata cgcattgccg agggcatctg tcatctttgt gctgttgccc tgtgaatcgt
    20941 attcgtaagt ggtctttcct ttcagggcat ccaccgtctg ttccagattg tcggtggaat
    21001 catatttcat tgctgtaatg ttgccctttg catcgcttac cgatgtcatg tttccattac
    21061 catcgtaagt catggtactg gtatttccat tttcatcggt agaggatacc agatttccat
    21121 ttaagtcata cttcatggat acggtatatc caagggcatc tttggatgta gtcagatttc
    21181 ctgccttgtc gtaagcataa gtatctgttc ttccctcttc atcggtagaa gataccagat
    21241 ttcctgcttt atcataagta tatgtggtag ttccaccttc tgggttggtt tcacttaagc
    21301 ggtttcctgt attatcgtag gtataagtcc aggttcttcc actgtcatct gattttgata
    21361 ccagattgtc tgccgcatca taagtataag tggtaactgc acctaattca tctgtctctt
    21421 tagcgagtct tccgcagtta tcatattcct tggaatggct gcttccatct ccataagtaa
    21481 tcttggtttc atttcctaat gcgtcatatt catatttggt tatattacca actgcatctt
    21541 tggactgaag cacctgtcca agagaatcaa acttggatac ggttgcattt ccatttccat
    21601 cggtatattt tttcagattt cctgctttgt catattcata gagctttgtt ccaaaatctt
    21661 tattactctc ttctatgaca cgtcccatgc tgtcatactt ataagtaatt gtatttcctt
    21721 ttgcatcggt tgctttgacc tgctgataat tcttatcata aacgtagctg atctgatttc
    21781 ctttcggatc tgtaccactg gtcatgttgt acatcttatc ataagtataa gtggtggtat
    21841 tccccagtgc gtcagtactt gctgtaatat ttcccacacc atccagcgta taagcggtca
    21901 caccgccatc ggctgctgtc ttggaagtca gattgccatt cgcatcgtaa ctgttcgagg
    21961 ttacatttcc aagtggatct gtcatggtaa ctgcacgatt catcgcgtca tacgtgaatc
    22021 cccatgctgc tccatttcca tccacatatc cggtcatgtt cggtccgtca tacgaatacg
    22081 ttgttgtgac acctctgccg tctgtctggg ataccatacg atgtttgtca tcgtaagtat
    22141 aggtaatggc tgttccatcc ggttttgtag atgtgaccat atttccatta ccgtcataac
    22201 tgtaagtaac cgttgctccg ttatatccag ttgcactggt cagcttattc tgctcattat
    22261 aagtataaga ggctgtcttt ccatcctctc ttgtctccgt tgctacattg ccaaatgtat
    22321 cataagtgta agtttttgtt ccggctgctg tcgtttcgga agcaagctga ttctctgcat
    22381 tataagtctt ttcacaggtt gttccatccg gatattcaat ggatgttgtg cggtactgat
    22441 catcataatg ataaacggtc ttatttcctt cgttatccgt tgttgtagta gaactctttc
    22501 cgtacttgaa agttgccgtt cctttttctg catcagtctg ctctacaact cttccttctt
    22561 tatcgtaagt attctttaca accgtattgc cattctcatc tttccagctt gtcatacggc
    22621 tgtcatcatc atatttgtac tcttttgttg ttccattcgg atctgttaca gatatcagat
    22681 ttccctgctc atcatatttg taagaaactt ttccaccatc tggaagagac agttccttga
    22741 tatgcccgaa tttatcctgt tttaccccga atgtttttcc ggatggtgtt gtaatcttac
    22801 tcagattata atcatcgtca taatctaata ctgtcttaaa tccattcaca tccgttacat
    22861 aacgtaaaat tccatattta tcaaatgacc agacgctctg atccgcatct gtcagttccc
    22921 atccgatgta gtcatggtct gtatctttat agctgactgc ttttaaatca tatacataac
    22981 catccggagt agtgtagctt ccatcttcat tcttatcaaa gatcagataa cttccgtccc
    23041 ctctcatata cagaagactt ccgtcctcca tttgagaaag tgactggtca tagttaaagc
    23101 tccatccacg tccgaacatg gaatgctggt ctgttccttt ggaattgtag cttcttgtaa
    23161 tggagaattc tccattcagt tcattcatcg tcgcatcgga ctgatccatg tagaagttac
    23221 cagtatttaa gttgactggt tcaaatccaa attcacagtg caggcctctt cccatcaatg
    23281 ctccgtcaat ccgcatctta tcctgatcgg taagcggtgc aggactatat ggaacatttg
    23341 tctgtgggtt acggataaag atcgtgttat ttgcgactac cagtgcgtcc ataacctgat
    23401 tgtctttcat aatgttctta agcggaacac cataatattt tgcaatcttc gggaatgtat
    23461 caaactgctt cacttcatag atcacaaagc tgtcactggt cacttctttt cctgtttcca
    23521 gctttccatc aatctctttc gatgcttttg ctttgatctt ataaagcttg tccttcgtca
    23581 gccctgaata cagtgcactc tgccagttgg aatctttgct gtaatactta ttcgcctccg
    23641 ggaactgctt attgtattca gtactgtctg gataggaata caatggtttt gcatctgttt
    23701 cgtgatgttc ttcattttcc tcatctggaa gcagataata ttctactgta gatttgctct
    23761 ttgcaattcc gtctgcaaat acggcatcaa acttcagctt tccatttgta ttcttttctg
    23821 tcatcgggcg aagcaggatc gttgtttcat ccagtgacat atctcgcaga tatggatctt
    23881 cagccggtga ccactggatg ctgagtttcg gtccataaac ggatgccctg ttattcagta
    23941 cctcacactg catagaagct ccaaggttat ttgcctcggc aatggctacc agtgcaaatc
    24001 cgttattcgc atgagttccc tgcacccagt cattgaccag atctttgacg tcataattga
    24061 tatagctgtt cctgctggta cttgcattct gtacatcctg aaacgtaaga tttactggtt
    24121 ttgttttcca tgtgatggag gtgttccacg actgatctac acgataaagt ccaaactggg
    24181 atgcaccacc actgtatgca gttttctgac tgactgcaaa tgttgcactg tcaattttgg
    24241 aatctttcgg aatctgacta aaatttgaat ttactttgat ataagtccgg cagttctggt
    24301 gtgcagtacc aaatcctgca aggttcccag acttaatacc atcatcaaat ccgacatacg
    24361 gataattgtt gtcaccaatc tgactggtag gacttccctc ttccacaccg atcatgttaa
    24421 aggaactctt ctgaatctct actggtgtcg gatcgatcct gaccggatac tgacgttctt
    24481 ctgccgaaag ccaatcccta tctggtttta cggtcaggat tgtttttcca tcttcttccc
    24541 gaagttccaa tgtgatcagg aaactgattt ccccggcagc atcttccatt gatggcgtct
    24601 ccagaagata cttcgcatct ttgattgtct tcccttccgg atagatatag acctgattgt
    24661 cttttacttc tgcctgatac cccggaatct gcagctcgta ttcatagact tccctgtctg
    24721 ttggctgctg aagtacgata tcttccttaa tgctggaatc cagtactgta tactgaacat
    24781 ctgcgccctc gtaaacttca ttataaagga tcgcattgtc tttaataacg gaatgtgtat
    24841 aatctccatc taccggaatg atcccgattc tggtctttcc attttcaatc gttacaccat
    24901 tctcttctga catctgttcc ggaagaagga tagcataatc gtttgccttg ttctgataaa
    24961 cttctgtttt ctcttccttt tcttcagttg ccactacact gctggcttcc tgtgcttctt
    25021 cggatgccgg agtatctgct tcttctggtt ttacaagtgt attatcaacc agctcggtat
    25081 ctccatcctc attcttataa gttccgacat aacctccata aacggttgta tactgcttgt
    25141 cccctgtctt ataagtcttg gaaatggcat cataatcaat cagttcacct tccggttctt
    25201 ctggaatcgg atagtaatcc tgtggttctt tcaactccac ttctttttcc tgcggatttt
    25261 cttctgtttc ttccgctgga gttgtctgtt cttcctgcgt ctctgtatct tctgatggcg
    25321 tcgtttcatt ttgtacgtcc gcatcagact gctgactctt atcttgttct ccagatgtgg
    25381 aatctgtaat ccctgcttcc tcttccgtca aagcctctgg tctggagaaa tctgcattct
    25441 tctgtacatc ctcaatggcc tcttttgcct gtacaaaaat aggctctgtt ccctgtagtg
    25501 ccatcaggag tgcaagtact aaggctatag ccgactttcg aaatctcttc atagtctctt
    25561 tctccctctt gtaatctttt tctataattc ctttacaaaa taaccgctac cctccttttt
    25621 tctgactttg gcactactat aatctaaaaa atcacaaaat tttcataaat ggtaaaaatg
    25681 ccccttgagg tggcaaaaat agacgaaatc ggtcgaaaaa aatagtaaga caccatcgcc
    25741 ttactatttt tcatctaatt tcatatataa taatattttc acttgaaagg atttctccgt
    25801 atggcagatc tccatctgac catgatattt ttctactacg agtttcacat tttgtaatcc
    25861 tattccatgt ccttctttcc tgcttttcgt tgtcagataa attccattcc gcacttttgg
    25921 tgtatctttt atgctattct caatctggat attcatgatt ccttttccat aatacatctg
    25981 aaacgataat ctcctttcat ctgaagctgc tgtcccacga atagcattat ccataagatt
    26041 tcccactatt atacttatat caaataattc ttcccctata tgattcggta tttctacaga
    26101 aatctccgga ttttcaatta ctgatttcga atcttccaaa atataattca gcagattatt
    26161 catattacgg ttttctgtat ataaatgatg acataaggct gttccggcta tattctccat
    26221 ttgtttcaga tacgatagaa tctcttcatt tcctcccgtt tttgccattg cccctattgt
    26281 tgtaagatgg tgcttgtaat catgatgtat cttattcagc ctgtcctgtg atttcatcat
    26341 catctccagt tcttttgcat actggctcat ctgaattttc atctcttcct gttttgctgt
    26401 ttcaatataa cttttctgaa cctcatcgtg cagatagaaa ataaacacat tgataaacag
    26461 taagaataat acaatcgtag accgcacacc tcttctgtct ggcagcaata tcctgataca
    26521 gactataatg ctgatcaccg ggacagaaag catagcaagc caatgacggt tctttatata
    26581 ataacttctc tttacaccta cttttctttc aataatgact tccagtaaaa agaatataaa
    26641 atttcccatt acaatagttc ccggtcccat gtgatgatgc accggatttc ttgctaccgc
    26701 ataccgttga aagaacacga ttggaatccc atcacataaa attcccataa tatataccat
    26761 aagtgtattc cagacatgat gttgccagtc tgcctgatat gccactgtta taattaacag
    26821 agctatcagc tcaaagatca gatctccgta catcgttccc cactgcatgc ttccccagca
    26881 tgaaagcaga tatgcacaaa aatatgcaaa tagcttttgt gtatcctcat tttttggata
    26941 caggatggta ttgtaacgga acacagtata aatccagaat aatttacttg tcagataagt
    27001 gatcaaatgg atattcataa tcttttcttc tccgtttgta atagtctttc cctgaccttc
    27061 ttgcgatttg ctttactgat acttagacgt gtcttgtctt tcataaccac gttgtcatat
    27121 tcgtaatgct ccacgtgttc catattgatg agataggact tgtaaatcag taagaaatat
    27181 tcttctgtta catccgctac atcctttaat tttcctcgat attcatattc tttttccgat
    27241 gtcacgatat gtactctttt tcccagacta taaaaataca ggatcttgtc ataagaaatc
    27301 ttgtggattc cttttgtatc cgtatattca aatattttct tccgttcttt taacagttcc
    27361 cgtaattcac ctatcacccg gaaaagttct tcctctcgga ttggctttac aagaaaatca
    27421 aatggatgaa tacggaataa tttcatagca tattcctgat atgttgaaat ataggtaatc
    27481 cgcagttctt ttccataatt tttctttcgt atctccgttc cgatatcaat tccattctca
    27541 tgttccagac aaatatcaag gaataacata tcgtaggatt cttcttccag cctgttaaga
    27601 agctgctccg cagaatagta tatttctata gatatctgac agtcattcgc tttcccataa
    27661 tggagcagct tctgttctaa ccaattacat aggatgatgt catcatcaca gattgcaagt
    27721 tctagcatat tttcttaccc tcagtctttc gtaacacgac aaatggggaa aagaatcccc
    27781 ttcaattctt ttcccgaatg gacatcacta attttgtgtc atttttccga tgcaacagca
    27841 ccacttcctt tgctgtctta ttttgtcctc tatataataa acacttgaac aagaaaaaac
    27901 tgtctccatt ttataattta tttatatttt ttatgtttta tttatatttt atccaaatat
    27961 tacagcgata actaataatg tcaccccctg cattattctt tttattttta gcgtcatttg
    28021 ataaaacatc aacgtttttg atggcaaaaa gggaacctgc aaaaccagat tccctttaaa
    28081 atacatgctc ttctctaaaa ggatgataaa tccaccatcc tctgcttatt tttaaaaact
    28141 ttttcctttt ttacaatact tataaatatt aaatggtttt tctttttttt caaataaaat
    28201 ccctgttgtt ttaataattt ttataagttc atccgactcc atatctgtcg ttttaaatat
    28261 aaatattttc ttttcatttg ttatgaaaaa aaagaagttt ttatataaga acattctatc
    28321 aatattttta aattcataaa tttgatcttc atctatttta attgcgtttt catcgatatc
    28381 caccgtttca tatcctgatc taccccagac aaattcatga acattttttt caaaattttt
    28441 accaatcatt tgataccaaa taatccaagc cacacaagcc acacaagttc caatccctat
    28501 tccaaacatc aaataacttt ccgacagata acaattaatc gacagtaaaa attctacagg
    28561 tgtaaaaata tagataatca ttctactttt ctgtcttgtt ttaaataatt gattaggaat
    28621 tataaaccca agcgaaagca ttctttctaa ttcttcccta tcctcatctg acacaattga
    28681 tttaatatgc attcttctta atcccccttt tgccagaggt cctctttata aaatcgaacc
    28741 tctggccatt gtctctttta ttcttttaca atttacagcg cttgaatcaa caaaccaaaa
    28801 acagtacctg ctccagctga aaataaagca ataattgacg gaattgcatt tacaactaat
    28861 gcacccgcaa gaactaatat tacggctcct gccagtatat tactatgctc ttttgtgaat
    28921 tctacaacat taaattcaag accactatta gaatttaatg tcattgtaaa aatcaaagca
    28981 accgatattg tccactcttt ttcctcttct ggcagcaaat ctgatgtact aaatacaatg
    29041 gaaaactcta cactattggc aaaaacatta tttatttcaa aagcaatatt accagattta
    29101 accgataaag caattttttc tatcgtattt ccaaagttat ctgctccctc tatacctgta
    29161 tctgataaat ttgatgaaat ctccataatt tgatttttgc aagtttgtgt caattctcca
    29221 tcatttccta tatccacttt tatattataa attttaccag ttgaactttc aagcgatgtg
    29281 gatattttag tagaaatatc aattgctcct tgaggactca tcattgttcc cagtgaaatt
    29341 tccttatcat actccacacc cacatccata attttattca gataaccaag cggctccatt
    29401 acattataga catattgatt tcgtgctatt tttacctttg cccgaagatt ttcttgcgct
    29461 atttcctcat ctgtttttgt cgatacggca tcgaactttt taaatccagt atctctccct
    29521 gaatatgcat ctttatccag tgggaacgaa ggtgatgaag aaaatgagtt caactcacaa
    29581 aattgatcaa atgcccagtt cttcggcatc gaatatccca aattacagct aaatccagtt
    29641 gacatatctg ccacaaatga tttacttgcc aatcctgctt cataaacttt agtacataca
    29701 tatcttggtg cataaatgcc gactttataa tttttatcat ttgtcgaact gaaaaagatc
    29761 atgtgaatct gttcaaaata aggtataata aatgtatcaa tctgatatga ataacaatca
    29821 aaatccactg caaagtatat agtcgttcca cttggtatac cgattctctc ggctgctaaa
    29881 attgctgtct gtgcatcaac acttccctgg gacggatcct taaaatagtt taattcatat
    29941 cctccatcct gatatatcgg gaataccgat aatcctgcat tttcaatgtt tttcacttct
    30001 gttgatgtca aatatttcgg tgtatgttct tttccaacag aacctgtcag ataacgtcct
    30061 acatgagtat accctgcatt ttttatatct aatgcctgtt gtttatttaa tactgttgca
    30121 cagtcacacg cttttgctgc cctgtttgta tcgcctttac tcgtaagcaa tgatttcata
    30181 gtagaaacat ttacttttcc ttttgttaca agtccaattc ctgtcagacc ataaaactcc
    30241 tgaaattctg acacctttga ctctgtagtc gaatcaaaca cgccatcgaa tcttcccgga
    30301 ttatatccgt taaaatatag tccgtattga gcaatcttat taaatggaac atatttcgtc
    30361 gagttctgtc cgttctgtaa tgtacccgga aacgcatttg ttgttgcatc gccaaaatta
    30421 actgaattca aatctgtaat taattcagtt gtaactcctt cagccgcctg caatgcacca
    30481 actaaagaca aaattgtctg tcttgaggca attccatcac aaggtccaac accaataaca
    30541 tctgaccagt cactatttaa ttgctgttga ataagaacaa tattggaatc cccaccagaa
    30601 acctttgtaa accagtttag ggataacaat ccagaccaaa ctttccaatc aattttgccc
    30661 gtaacttcca gtcctgcatt ttcctgcatc tttttaactg catttactcc tgatgtataa
    30721 taaattccag taattcctcc agcagcatat cctttgcaaa acagagcaca ctgaatcaag
    30781 catacattaa cctgcggagt atcattcgga tccatttttg taataatggc taacttcttc
    30841 atggtattaa tagtaagtgg tccaaccgtt cctgtaatcg ttgagattcc attctgaatc
    30901 tgaaatgcac gaatgattcc ctgcatcacg gctgttccgg tctttccatc ttcatccagt
    30961 tttacccaat ccttatgtcc accaaacatc gcatttaaat atttttgtgc tgcttttaca
    31021 ttctgatcac tcatattcac tacctcctaa ttcaagtcaa ttataaagat ttctaactct
    31081 ttatatgtct atcactaaaa gttacaataa caatttatca atcaactgtg cctattttgt
    31141 gactattttc caaaggattc attaactccc ttataaaatc cttaacaaat tatgaaagaa
    31201 accatcaatt tatcacccca tatcccatta tagaactgta atctactgca taatttctct
    31261 gccgtacctg atcactggaa tttccctcaa tcgtgtaaac tcttccacct tcacactttt
    31321 ctactattcc tacatgatcc gatgttccat catgatccca gtcaaaaaag atgatcattc
    31381 cagctgtcgg agttgttccg ccactctgcc acttattctt tccctgaaac caggacactc
    31441 catcactgca caatgagaat ttaggaacat tcccacttgc aatcagaccg ctctgatccg
    31501 cacaccagct tacaaaacag gcacaccatt ctacccgact gtcaaatccg taccagctcc
    31561 agaacttctg accgccactg tttccaagct gccccattgc aaccgacaca atctgctgat
    31621 taccaaacaa accagcaaac aggctgccac cggaataata ccgcatcaca tggggaacgt
    31681 attggggatc tccgtaactg ctccatccat gcgaagctgc ctgttcctgt gaaaactgca
    31741 atgcattggc ttctgtataa ccgcctcttt gcaatgccca tcctatgtag ccatttccat
    31801 agttatatcc ctgccatgca agtttcagct tgtccagatc ctgtggactg ctgcatcctg
    31861 cctgtctgat acagtctgca aaggtctgta ctccaacctc tatcgaataa tccggatcag
    31921 taatagaacc aggagtgtgt gggaatcttg tattatatgg actttccgaa cactgcatcg
    31981 ggtctgttcc cctgcctcca gattcctgca tcatgatggc ctgtatcgca gacacatatt
    32041 ccggaatgcc gtattgggat gcatactgct gtatcacaga agtatacgca agcacttctt
    32101 cacttaggga ttcggagctt tccgaattac tgctgaaagc tgctcctgca atgattccca
    32161 caatcagaat aaataccaga agaaatgcac tacctccggc agcaatccat tttcccattt
    32221 tttcaagagc tactacccct ttttgaatcg ctgctgtaat tccctgtacg gataactttg
    32281 aaccctttcc agttacctga aggactgctt ttccactttc tgccgatgcc tgtgctgact
    32341 ttcttgccat ctgggctgct cttgctttct gcatcgcctg aagacttttt tccatctgct
    32401 gtttctgcat ggatgcctgt gtctttattt tttcttgaga cagaccggat acttttacca
    32461 ctctcggtgc tgctttcacc tgccgtttac cattctcctt ggcaagtgct gcctgctccg
    32521 gtttcaactt gattaatttc tttccgcttt ctgccccctg ctccattgct tcttcggcag
    32581 ctttcgcttc ttcctgtctg ctttttcttt cctttatctt cctgtaggtc actccagcaa
    32641 gtttttttcc accttgatat gtggttttcc ctatactttt tcctgccaca ctcgccgctc
    32701 tgtactgtac ggattctatt ttttcagttc catactgtgt tggggattct gactgcccac
    32761 tgcttttttc atgtagttcc cttggctttt cttttgcttc cagaaccgct gagcgaacca
    32821 gttctttcgg aagtcgggca gccggatttc gaatcttcgg attcttatcc cttacccgtt
    32881 ccttgatatc tttcatccgg tcaccctctt tccgtatctc ttatctcatc gggcaacatc
    32941 ccgatgtttc tttttttcct gctgtttctg caactgctcc acctgtctga ttgcctcatt
    33001 taccagtgga tgttcgttgt aatatggcac aagttccaag tctcccctgt cttttgatga
    33061 caacggtaat ggatattcca tgtctgaata aattgattct ggatcatgaa tagaaaactc
    33121 aatagccgga cacatattgg cagatgttct acaatatttc tggtactttt tatatgcctc
    33181 ttccagcgtt ctgcattttg tatattctcc taaatcatgg aactcaccgc actctgcagc
    33241 ataaaaagtt atcgttactt cttctttttt cttcattatg attctcccgg ttttgttgtc
    33301 attagtttat aaaggtctgt attctttggg aacgtattct caaatggtac gatattccca
    33361 gagcaacgga ttaggccact gccaaccgat acatttgtaa tataggacag ttggttttcg
    33421 gaaatattca aaagagctgc caattcttcc ctgtctgtac ttgcctgatt caaaagaatc
    33481 aaaaactcac tgtttgccaa catcagtctt gcagtgtccg atttcagaag ttcctcaaca
    33541 ttttgggtta aacctgtcac gaatccattg tattttctga ttctcttgta cagtcggtag
    33601 aagaaatttg cactgtattc atagcggaat aacagataga actcatcggc aaatatccat
    33661 gtcgtttttc cttctttcca gttctgaatt accctgttga aaatactgtc taaggtaacc
    33721 agcattccca gtggcataag ctgttccccc agttcccgga tatcataatc catgatcctg
    33781 ttcttcttat tcacattggt cggctgtgca aaggtgttca ggcttccatt gataaaaagt
    33841 tcggatgaca gtgcaagtcc ccttgcctct tcttccggct gtaacatcaa ctgtcggtac
    33901 atatctttca atgtcggaac ttctccggta taaccacttc ggatataatc ccggtacaca
    33961 tctgcagcac agcggtccag gattgacttt tcttttgctg acaggtttcc tgctccaacc
    34021 aactgttcaa atacagacag aataaactcc gatttttcaa tcagtggatt cttttcattg
    34081 ccgtaggctg cgtccatatc cattgcattc aggtgattgt ctgatgtcgc agatactttt
    34141 acaacctgtc cacccagagc ctctactaat tttgtgaatt cggactccgg atcaagaatc
    34201 aggatatcgt catcggtact caatgccaga tgcacaatct cttcttttgc cgaaaagctc
    34261 tttcctgatc cactgacgcc caaccggaaa ctgtttccat tcatcagttt cttccggtcg
    34321 gctaccagaa ggttcttgct gactgcattc tgcccataat agataccgcc cggctgcagg
    34381 atttcctgtg tatggaacgg gattaaaaca gccgttgatt ccgttgtcaa tgtacgcagg
    34441 gcattgatct ttcgcagtcc gtaaggaagc actgtattga gtccgtccac ttgctgccac
    34501 tttaatgttg ccatctggca cagatgcttt cttgctatcg acaataataa ttccgtatcg
    34561 gaatccaact gttttttact gtctgccaga tgtaccattg tcaaaattcc gaacatcata
    34621 cgctggtctc tggttgtcag atcatcgagc atttccttgg tttcttttct ctgaagttcc
    34681 atatcgtatg gtacgattgc cgagaagtta ttattagcgt tctgccttct ctgccagttg
    34741 gtcacgttgg tttccacacc aagcagtcgg ttctggattt cccttaccgc ttcatccgta
    34801 gggactggca ggatatcaat cgacaatatc agattccggc tgaagtcaca cagttctgaa
    34861 atcatgtcat ctttaacata acttgcataa tcctgcatat acaggactct tccgtacctg
    34921 tcaccgattt taaaatggtc acgttcaaat tccatagaat ccggacacat ccagtccttg
    34981 aaacttgttc ccttcttggc aaattgtttc agatcaaacg gaaatgcctg tgggacatct
    35041 cccttgaaga agtccctgaa aatctggagc cggctctctg cgtccagtcc ttctgctgtg
    35101 gacgataact ttgccagatg tgttacgatg tctgttccga tacgtgcaaa ataggttctt
    35161 gcatcgtcaa tcgatctttt atgtacactg acagtcagat accgttcctg atagatgctg
    35221 ttgtttgttc ctgtaatctt agacaggagc atttcgttgt attcttcccg atagtggtcc
    35281 agtccatctt ccttcattgg aagcaggatt gatttttcaa attcttcttt attgattctc
    35341 cggttattga tggtaatttt ggcagaaatg ccggaatcca gtgaatttaa gagttcggaa
    35401 taatccagaa acatttctgt cttattgtcc ttactcgcaa tataatagtt gatatctgta
    35461 aaacgatatg tttttgagaa tttcgttccc tgttgaaaaa ttccatccgg ccagattcgc
    35521 tggatgggaa ttgcctgttg gactgatttc ggaactttga agcgttcctt atccatccga
    35581 agtgcctgac ttaaggtttt aatcaaaggc atcactcctt cccttgcgtt tctttctctg
    35641 ttttgcttct gctctttgct cccgctcttt tatgatttcc gctccatcct tttgattctt
    35701 ttctcccagc gagatggtct cctgcatgca ggagtagtgg atatcttccg actgaaacac
    35761 cagtttcttc ggatacagca attcggattt gatcactgcc cataaaaact gttcggctgt
    35821 cataccatga tatttgaaaa agccacaggc agcaaatgga gtagcaccaa gcacacacag
    35881 ccatccggtt atttcctgtc cggtaatttc acgcattcca aagtaaattc cgattgctgt
    35941 aagaatcgca agaacagaac acacacattg tctcaggtcc agtcccataa acatggattc
    36001 ctgatagtcc cggatttctt tattcatctt gacttccata acttatcgct cctttcctct
    36061 gctttgtttc ttcgttttct gtggtttttc tttcggtact tctatcccgg caaatctaca
    36121 gtgttctgca acgccttccg gatcaaattc cgcaagtctt gctagatcaa acgctacctt
    36181 ctgataggtt gcatatcctg atcttcctgt ttcattcaac acttttccta gtttatgttc
    36241 cttgataaaa tgaagcatat ccttggtaaa atctcctgtt ataaaagcct catttggctc
    36301 taagaactga tagggcagat taatggttac atctccaaaa tcttcccatt ctctcccttc
    36361 cttatggcag agtcctacat ataaatttcc attgttccga taagagctca ctcgtattgc
    36421 tactgtctca cgtccaaatt cccagttata tcttaatgtt ttctttatat ccatattttt
    36481 aacctccaag tcccatcatt tctctgacaa tacggtcaga tgccttaatt gctccaacca
    36541 gcaccagtaa attaaatacc agttctccga catagttcca cacgattgta actgcactca
    36601 actccgtatc tccgacagcc ggagggcttg ctgcataagc agaaaagata atacatgcca
    36661 gtgcaataat tgccccttcc agacatacgc ctacataaga acggataaag ttctttccaa
    36721 tggactgcgt gggttctcct gcaaatgagg aaatcggaat cggggcgatt gccgtataca
    36781 tatagatttt gaacattctg ccatacactg tcaggatcat tacaaatgac aatacagtga
    36841 tcagcagact gccaagcagt gtaacaatcc acaacggaat ggattccagc attcccacgc
    36901 tctcaatctt atctacaatc tccgttggca gttccgtcac gcttcctgcc atactggagc
    36961 cactcattac ggttgtcacg attccctgca caatggaaaa cagtgcctgc ataagttcca
    37021 tcccgtatgt aattgctccc tgagcaagtg caaaccggat aaatgctttt agcacatgtt
    37081 ccggcttttt cagatctgta aaactgccac acgttttaat gattccggct gcgaaaaata
    37141 ataccagtaa tccataagca atggctgtca gtgcatcatt aatatttacc ataatccgcc
    37201 agatgccgcc gcccttaaaa gttgccggat tttcggtcag cagagtccac agttctgcca
    37261 tcttttcact ccatgtggat agtgctgagt ttagattctg tacaatccag ttatcactca
    37321 aaaactctca cctccttaaa catatttttg aatacaggag taccttccgg cactcctgct
    37381 gtttcctgat atattttttc aaagaggctt acgccataat caaatcgagg atttctttgg
    37441 cgaacgtaat aattacccct ccggccagtg tcagaaatcc atttgccctc tggcttggat
    37501 catggctttt taaagacaga ccaacctgca caacaccaaa tccaagcaaa atcaaaccga
    37561 tcgcacgaat cagagaaaaa ataaaggtag acaggttatt gataacggac aacggatctc
    37621 cggctgcgaa tgcggtcgtt gctccaaccg ttacggtcag taccagtgcc atataaaggc
    37681 tcatccactt tttctgtttt ctccccatcg gcatcggggt tatcattttt tcagttgtat
    37741 tcttttttgt tctatttttt tctcctgtga gaagtttctt caatacacat cactccaatc
    37801 catcaaaaaa ctgctattca ccaaggatgt cctcggtaga cagcagttcg taatcgtcta
    37861 atctgttaat gtcaatcttt aggtcgtcgt gagccagtgg tgttttcgca taatcataag
    37921 gcgatgcacc accgtcctct gtgtatctga aattcacatg cttttttaaa tcatatttat
    37981 cgtccatgat cggacgctcc ccacgaataa acaaaatcgc tttgcggtta tctaaaagcc
    38041 gtatttcatc cggtgttaaa agttcccttc ctgtctgctg ataattcacc gaatagctgc
    38101 cgctacgccc ttttgtctgt ccgtaggtat tggtatccaa tgtctctttt cccaataatt
    38161 ccgagacata cttatgtcct tctttttcat ttccacccag atataaaaat tcatcgcagt
    38221 tgccgatcag actctcatac gagtctttaa ataaggcttt catctgtgca atgttctgga
    38281 tgatgatact gcaggaaatc cctctggaac gcatggttgc cagaatcttg tcaaaatcat
    38341 ccggcaaggc tacgtttggc cattcatcca taatgcagtg taccggaatc ggaagccttc
    38401 ctccgtactt ccgatctgcc acataataca gtgtctgaaa cagattactg taaatcatgc
    38461 cgaccagata attcatgctg gtgtctgcat ccggaataca gcaaaacagt gctacttttt
    38521 tctccccaat actgtataag tccagttcgt ccgtacaggt caggttggcg atttgtttca
    38581 gattgaacgc tgccagacgg acaccaaccg atatcaaaat agactttgcg gtctttccgg
    38641 ccgcctgctt ataaatggca tactgcttga ccgcaatact ctctgggtcc cgcatttcca
    38701 gtctttcaaa caaaatgtcc aatggtgact ggtaatcctc atcatcttct ttgacctgtg
    38761 cgcttccaag catttccata atcatcggga agttctgttc ttccggtgga gcttcatgca
    38821 gtaaatacag cataagtgcc tgtaaaagtg cagtctctga cttttcccag aacgggtccg
    38881 agctttgtgc acctttcggt gtcgtgttgc ggatcaggtt gttaattagt ttcagcacat
    38941 ccttgtcatc ccggacatac gcaaacggat tataacacca ggaagtctcc ggattgatca
    39001 aatcaaaaac ccggacttca tatccattcc gttccagaac acctcctgtt ttgcgcagaa
    39061 gttccgcttt cggatctgtg atcaccatcg agcagttgca ctgcatgatg ttcggaatcg
    39121 cataggtccg gctctttcct gccccggaac ctccgactac cagcacattt aaatttcgtt
    39181 tatgcttata tccatccagt cccatgcgga aatgctgtgt cagaagcaga ttattcgtgt
    39241 actgcttttc gcagtaacgt ctgcagattt cggacacgtt gccccatttt gcggaaccat
    39301 gttccacgcc tcttctataa tttctttttt gggattcata aataccaatg cctaagaaat
    39361 aggctgctgt gaatatcaga atacatttga acgtatacgg tgtatacgtg ataaaaaacg
    39421 gctgttcgag tttttcgctc agcgtttcta agatttgaat aaaattctta tccggtgtaa
    39481 tggcacaagc ggtaatcgct gccgcccacc agataaatgg cagaaagaca agatacagaa
    39541 tccagtcctg cttttttgta cttatctctg caagtcagat tctcttttct tgcgtggctt
    39601 ttcttctgga tgtccctgtc cttctttctt atgaaaggaa gccactcccg gtatcaatga
    39661 gagacttctg ccgttgtccc acttaacacc aagctggcct gcatcatcca catactgcac
    39721 agttccttcc agtccctgcg gcatacccgc ttctccatcc atcgaattca gacagatttt
    39781 tgttcctgcc ggatactgtt ctctaagtct tgccactttt tctcttgaat aaatgaccaa
    39841 tcaaccactc ctttctctac cagaacatgt gtctgctagt attcggtttt gcaacctgga
    39901 taataacttt atctattcca tcgctgtatc ttccggctgc aagatattta cttctgagtc
    39961 tgttcagtgc cttacggatc tgcctttgtt ccccggctgt aatctgcagt cgtttctgag
    40021 cgaggtcgta ttcacttact tttttcagaa gagctttcgt ttcttccaaa cattcatcct
    40081 gctcactctg tgcctgtagc tcatcattca gtgccaccac gaacatattt ttcataatgt
    40141 tatccatctt caaatagtat tttgtcatct ggctgacctc ctttttttgt tacaccaacc
    40201 atatcacaag acctccgtaa tagctactcc aaccgtccaa ctttttcact tttatcgttc
    40261 cggctggctc tttgcttttt tctcttttcc ggatgctttt tcagatggtt tttcatcctt
    40321 ggtttccggt atttttacag ggtctccgat tgcgaactca tcggttatat actcctgttc
    40381 ctttgcacca aaattgcgtt cgatatggtt tcgtatactg taggaagcct gacggacatc
    40441 atttaaaaag tctctcagtt cctgcggatc tttctttccg cattcattcc tctgtgcaat
    40501 atactccaga ttgaatccgg taacatccac accataacgt ttcgcaatca cataggctgc
    40561 acaataagcc tgtgcactga ctttctgtcg gctgtaggtt ccagtatgct ggtccagtgc
    40621 tgcacacgcc agttctctgt tcagtgcatg gaatgtcgtt acttcactca ttccgttgcg
    40681 gatataaatg gtacggtatc tcggattgta ctgggcctgc actttgtctg gcagatcatc
    40741 ctcaatctgc acacgtaccg actggtcttt taagacagtt cctatcagtt ctgtcagact
    40801 tcgctgaggg cgttcttcca atggctttcc tgacatctga ctgatgtcaa atccttttcc
    40861 gatcgtatac cctctccgca tttcaccgtc tttttcgtat tcagtggaaa tcatataagt
    40921 aatgccatgc tcccctgctt ttactgaacg gtgttcttct ctccagcgtt cataagacct
    40981 tactacctta aaatctggat gctgtccgta aatcaaaagc agattcggag ttctctgtgt
    41041 attacactct gccataaaat ccagaaagcc tttcagtttt tctccatcct gaaaaactcc
    41101 ctgtgcctgt gaatctactt cagcccatac ctcttctctt tcctgctgct tcttggctgc
    41161 atattcttcc ttggtcaatt tctgttcagg aatctgctgt tcttccgttc cacctgaaat
    41221 catatctgtt aaatccatat acattacctg cctttcgttt tcatattctt acgtttcttc
    41281 cggctctctc tggcattttt ccgtctgtct ggacgcttgc gtttctcttc ctgttcctta
    41341 cggatatctc cgagttcttt ccgcacagat ggtttttcat tcttttcttc ccagctagtt
    41401 tcccgaccgg aaaaaatatt tttgttgcgc aagaaaggtt cggatggatt ctttacttcc
    41461 ccctcctgcg cctgtgtaaa atttccttcc ggaaaagcct catccccgat ctgaaacatt
    41521 tcttccgcat catccagttc aaattctatc ttgccttccg gcaggacaac ggtctctgtc
    41581 cgtacattct ccccggtttc tttttccatc tggttctctg ccttctccgg tgcattctct
    41641 ttttccactg cttctgtaac catagagcct gattctgctt ttacaaaatc cagattcatc
    41701 cggtctaata cccggttcat ctttgctgca tcatctgcaa ataccatgac ctctgtctga
    41761 tccggattca ccttattctt gataactaca tataagatgc cccttgcctt ggcctctttt
    41821 gcaaattctt tcagacggtc acccggaaca gtaaaaaatt tcatcgggcg ctgttccttt
    41881 aacattctgg taagtcttgt tttccctctt gtctttttct ggtccttcaa agcggcagca
    41941 ataaagactg ccacatgctt tgctacattc ccggtgatct tcagcgaata ctccatccct
    42001 tccagactgt accgtaccac ctgatctgcc ggttctgctc cgtaattcat acttatctca
    42061 cctcctgact tttctgcatt ctttctttat cttccacacg tttttcatcc tgactctgtt
    42121 cttctgcttc ctgaagtctc gcttcgattt ccaaggaatg tttctcaatc tgaacggtca
    42181 tacgaacttc cttacggagt cgcttcaatt cctcattgat ttcggacaat cgttcttccc
    42241 ccgtcttgct gcccccattc cgatacaatc ttctgcgttc tttcataagt tccgcaatct
    42301 gcttttgcaa tggctctcgg tatgcggcaa gctgctctct ggttatgatg tcatgtttca
    42361 tcagaaattc aatctgttca atcctgcggt ccagtttccg gatatcctct ctgacagcgt
    42421 atggactcct tttcggtctt tttggcagta cccccatttg atataaatag gaataataca
    42481 gtgcctgaat cccggtcagt ttctttttgg gatgaatctg tactgccttt tgatagactc
    42541 gtttcacctt gggctgtagt atctgcccac gtatttcctc ttcagaataa ttttttccaa
    42601 gggaacgcag acgaatatac cgctccattc ccggagcttt taaagaaatg tatttcctgt
    42661 tcagtttcca tgtataaccc cgcttttcca tctcttttag gaattgtgac caggagtagc
    42721 tttcctgtac cgattccctg atatccaaac ggatcgccgt tctccatgtc ggctttcctt
    42781 cctgttctgc tttccactgt acataaggga ttgatttttt ctgattggat tcaatgatag
    42841 acagaccata ttttctgcac aggtcatcgg aaatcttacg gatctccgta taataactct
    42901 tggcattact atggtactta tgtccgtcca ccatgcttac agaattccag acaatatgat
    42961 tgtgatagtg tctggtatta agatgtgttg ttatgacgac ttcaaattgt ccttttaaga
    43021 gccggtctgc caattcctgc ccgatgagat gtgccagttc cggtgtcact tcaccttctg
    43081 caaaactctg taccagatgg tatccttcta caccgcccat cttatggaat ctctttttcg
    43141 ttgccagcat atcttcatac gcattttcat tggtacatcc gatggtatct tcgaagaccg
    43201 cctgttccgt cttggtccga ttcatggcat aatcgatggc agcctccaga gagtccgctt
    43261 tctgtgtggt tttttcttta tctttcacat attcgataga acggtccagg cggtggacgg
    43321 ggataacact tgtataagcc atccggcatt accactcttc ctttaccaga tgccatgttt
    43381 cttttgtaag ccgcagcatt tcctgaatac tctgctcgct tgccatacca gaagcattgg
    43441 caacatgtgc aagctgattg gcattgttgc ataagcctgc aatctttcgg agcaattcgg
    43501 gatgatgctc acagggtttt gcatgaacct gcgctcctaa aatcaacgaa cgcataaact
    43561 cttcccggct cagaccgctt ctttcgacct gctcattcag ttccctgact tcccttgcat
    43621 taagccgtac cgggatcaca tggtttcgtt ttcgcattgc atcaccgtcc cttctttttt
    43681 ctcttcctct ttcatcatac cgccaagatc ctcatcggat tttttcgctg caaccatgca
    43741 acaggtcact atcactccta taaatcctcc gctcatcaat gcgattacaa tcattactcc
    43801 atttcttaac atgacttgat ccttcctttc catttccgtc tgtgtccggg ggattggggt
    43861 tctccccaaa gtgcgtttgg atatccaaac gctatgcttg caaaacgagt accgccagtt
    43921 tctgtagcac cggattgact cgattctgat ttattttgat tcttttatct gtaccagtcc
    43981 atcaagtgtc gtacaaatga tatggagtct gtctgccata ctgatttcgg tattcattgt
    44041 tcctgtcact tctttcccac atacgactac catccggcat ctccgtaaaa ggatatggga
    44101 cattcttcca tatccctgca tatcttctgc atcagactct tccaagaatg gtgagaatac
    44161 aaatctcgga cagattggta catacccaag ttcatagatt tttctgcaat atttctgcac
    44221 ttttacccgg ctttcttctg ctgaacagca cacataagcc agcgcctgtt ccatacgctc
    44281 catctccttc ctgcatccag ttggatgcca ctcattttct ctataaaaaa caagatgtcc
    44341 aattggacat cccggctact tttcatcctc tttctgctct gcttctaatg caattttcac
    44401 tctgtcactt cccagtttgt agtaggcatc tgttacctca attcccactg cacgataacc
    44461 tgtcataacc gctgcaagga cagttgttcc tgctccgaca aacgggtcca ggatcagccc
    44521 tctcggttca caaatctgga taacatcttt cataagctgc aacggtttct ctgtcacatg
    44581 aatccggttc tgcggattgc catatcggaa gacgcccggc agacaagata ccggacggtt
    44641 gatcggcatc ggtccgttac tgccccacac aatatattca gcctgctggc ggaaacggcc
    44701 tttctgtgga cgggagtttc ccttatccca caccgctgtt cctcgccaga tccatcctgc
    44761 ccactgcaat gcatcggtaa tcgatggata ctgacgccag tcaatgaaca gacagatcgg
    44821 agcacctttc ttgcaggctt tccgcacatc atagagccat tcagccatcc agtgcgtcca
    44881 agatctctgg tctttgttat ccccatcgaa atccggaagt gcattggcag cactcatact
    44941 gctgtatttc tgatttgtag tacgatttct ttcattctgt tttgttccac cggatgcata
    45001 cggcggatcg gtaataactg catcaaagat tcccggctgg aatgctttga ccagttttaa
    45061 tgtatctcca tgtaatattt tccagttttc cccttcagta tattcctccg ggtatgttcc
    45121 ctctttcata agttcttcca aactcaggcc atttgtcata ggcatcatcc tttcttttct
    45181 cagcgttccg gctgattttt cttctttgtc tttttatttt ctttgggcgg cggactcaac
    45241 tcagtttcta catattcgct gataatcaaa agtgctttgt aatagtcccc actttccaat
    45301 acccttttac gcatttcttt tgcttctgct gtctgtttat tctttttcaa aagagttgag
    45361 gcatcgccca taatggaaaa aatattccca tcatgtccaa ggagctgtaa cttgggacgt
    45421 tgtttctcag gctttgtcgg aatctgctct gttttctttt catacatttt cggctgttcc
    45481 atattaggat gccaaaaatg cacattcatc gttccaccat ctaccttgat atccctctgt
    45541 tcaaatcctt caccccaccc gtcactgtac tgccctgtaa tgtattcggt gaattcctgt
    45601 agttcttcct gacttaaaaa ttcatttaat tccagtttgg tacagccgta caggattccg
    45661 tctttgtttt ctatcgtaac aactgcactt tgtatctttt cctgaatgga ctcacttccg
    45721 tcaaaatact gcatgagatc atcttcccca tagcgttcca gttcttctcg gatgacttct
    45781 tcataatcgg caagttctgt tccatctaat ggcattccat attcatcact ctccgaccac
    45841 tcttcttccc attcggattc ttctgaataa agatttccag tcagctccgt caacagatgt
    45901 acgtgcatcg ttcctttttc ttccattctc tatcaccttc tttctaatgg tctgtccctg
    45961 catttatttg tctgcttttt ttctttcgca aggtctgcat aatctgcccc ttctttttcc
    46021 ggcagattgc aaataatctg atacgttccc tcgtaatgtt ttttcatctg ttctttcgcc
    46081 catctcccgg caaagtcatt atcggtgcag atttcaattt ctttgatttc cggatggttc
    46141 ttcagaaact cttccagtgc atccggcagc tttttaaaac gttcactctg tatctgtgtg
    46201 ttttctttcg gagcagcgat tcctccaagg gaaagacgat atttatccct ctttccttct
    46261 tccagtgtca tgtgggccag tgcatcaata cacgcttcat atacgacaac tcgttttgtt
    46321 tctctttcag gtggaataca aaagctgtac tttttttcac tcccggtctg ctccatcttg
    46381 aatcctttcc cgtttttttc atagattcct cgcaggaatg catatcttgc ttttccggat
    46441 tcatcttttc caacaaaaac agcgttatgg tacgggacac tttcataaag gatttcatgc
    46501 gaaatgcaat acgtgatcac ttcatcgctg attcctctgt ggtttaaata agccctgatt
    46561 cgtctgcaat tacaatatct ctccggcagc ttaaagggtg gtttttcctt tttcgcagac
    46621 tgtgtcggaa taaaagacgg actctcttcg cacaacagtt ttacggcatc tgtaaatttc
    46681 attccatcta cttcaatcag gaaccgcagt gcggacacgc caccgatatc tctggatttc
    46741 caatgccatt tgctggacag ttcattgatt ttaaaactgt catgatccgt caactgccat
    46801 tcatttctgg ttcttgtctt ttgcaaccgc tgtgcctgat gcgtccgcaa atactcatac
    46861 gcagtcatat tttttgctgc ctgaatctct tcttccgtta cccagggcat tacagaattg
    46921 catcctgaag atccagtacc ttatccgtcc gctcccaagg aaactccttt cttccaaaat
    46981 gaccaaaaac tgccgtctgc cggaaaatcg gacggttcag tcgaagggat tccatgatct
    47041 gaacaggtgt cactccaaac accagtggga ttgctgccgc cagacaatca tccgcacaca
    47101 ctttccctgt atcaaaggta tccacctcaa tcataaccgg ctctgctact ccgattgcat
    47161 aagccagaga cacctgacac ttgcttgcaa actcagcagc tactatattt tttgcgatat
    47221 accttgccat ataagctgcc gaacggtcta ctttcgaaca gtcctttcct gaaaatgcac
    47281 cgccgccatg cggaaccata cttccataag tatccaccat cagctttctt ccggtcagtc
    47341 cggtatctgc atcaaaccct ccacacacaa attttcctga cggattgatc agaatcttcg
    47401 tatcctcatc cggcggcagt aatctgagtg ctgagcgaag cactttctct ctgatttcgg
    47461 catctaactt cttcaggctc ttttctgcac catgctggca ggaaacaaca acactttcca
    47521 gtctcactgg ctttccatct tcatattcta cggttacctg tgcttttcca tccgaaaaga
    47581 tatctttgat atatccgctt cttctgcaag ccgaaagttc acgtgtgatc cggtgtgcca
    47641 gaacagtcgg cattggcata agctgcggag tatcatcaca cgcatatccg accataacac
    47701 cctgatctcc agctcccctg cttctatctc ttaattttgc gccgatatta tcccgaaatt
    47761 ctttcgacac actcactgct ttcgcaatat ctggactctg ctggtgtaca aacgtatcca
    47821 tttctatccc atctgtacaa tatccacact cttctaaaac ttttcggatc acatcaaaca
    47881 ctggtggttc atatccgctg gtgatttctc ctgctacaaa cacatttcct cttgtagcaa
    47941 ggacttcaca cgccactctc gaagatggat cgtaacgcag acattcatct aagattgcat
    48001 ctgctatctg atcgcatact ttgtctgggt gtccttctgt tactgattct gctgtataaa
    48061 aacgtctcat cattcttctc cttatataca gacagggcag aagctgcttt atgcacatct
    48121 cctgccctgt cagattggtt tatcctgtaa ttcttatttt atttttcttc tgtttctttt
    48181 ttgttgtctg tcatttttga cttctttctg cggtatacag tcactcctgt agctgttcca
    48241 gcagatgcca gcgcaagcag aacaaacgcc catattttct ggttatcacc tgtttttggt
    48301 gcatttgttt tttctgttgt tttctccggt gttctggcat ctttcatttc tactttctgg
    48361 acttccattg tatctttcaa tgtaaatgtg acatcttccg caatctcgta accatccggt
    48421 gctgtgatct cacgcagagt aagttcttca ttgactggga gtttttcaac ggaatgtggt
    48481 tttccatctg taatccactc ttccagaaca gttccgtctt tgcggataat ctgaagcttt
    48541 gccccttcca gttcttttcc ggttgtaagg tctgtttttg aaatttccac tttggaatat
    48601 tcatctttca tctcaacctt tgtcacactg ccatcttctt tgacttcaaa catcacatcg
    48661 ctggcagaca catagccatc ttcataagga gccagttcct catgtaaggt ataacttcct
    48721 tccggcagtc cgtaaatgac atgttcttcc ttactgctta cccattcttc cacaacattt
    48781 ccgtctttat caatgacctg cagtttggct ccttccagct catttccggt agttgcatct
    48841 gtttttgtaa tgcgtgtttc tgtcggctga ttctcaactt ctttgttcag ttcaatcttt
    48901 gcaagattct gattttcgta agttgcatct acattccata cttcctcatt tggaagatat
    48961 ccagcttttc tgacttcctc tttgacatag tattttccat gtggaagatc actgtcgaaa
    49021 gtaagtgttc cctttgcatc cgttgctttc ttttccagca aggtatcttt ctctacgaga
    49081 acctctccct gattagaaag aatatcttct gcagcataca gtccaaaaat aacaccttcc
    49141 agcttttcgc ctgtgacaga atctttcttt tctactgata cgaaaacttt ctgtctttca
    49201 tttttatagg taacgccttc ataaacaact gcctgtgtat catcctctgc tgtcagggta
    49261 aattctttct gttctgtatt taggacaaaa ttctctcctg ctacaacttc tttcagataa
    49321 tacgttccca gtggcagatt gttgataact gctgttccat tttcatctgt agtcagtttg
    49381 gctaccaggt catctttctc atatcggaca actggatttc ctgcttcatc ctttgctccg
    49441 tccggtgaat agatggtatc tttggcatat atttcgaatt cagcgccttc tactccggtt
    49501 tcttcatatt taaagccttt ataaatacct gtttcttctt ttccagatac ggcatttctt
    49561 acttttgaaa ccagtgcttt tacttttgcc agaacagaat ctccggttac ttcggtaagc
    49621 tgttctccct ttttcgtcag gagcaggctt cctacctgtt catcgttttt ctgttctacc
    49681 tctacaatcg cagctcctgt atctggatca atctgatgtg cagtgtttga ggatactgta
    49741 attacaatac cactctgtgg attttcttta tatgttcctt ttgtagtctg ttctaatggt
    49801 gaaataatcg ttgttccatc atacagggac tcctcgcttc cctgtcttac aaatccttcc
    49861 ggtgctttca cttcttcgat tcggtaggta gagcacttca gttcctgcgg tgtgatcaga
    49921 tatccttcct cgtttgtttc gaatacactg atcttttctt tcttcggata ctgaacaacc
    49981 tgttccacat atttcttatt tgtcacatcg taaatcttat aagaagcacc agcctttaaa
    50041 acagtatttc cagtctgtgc atccttcttt acaattttca gcaaaaattc aaacgggcgg
    50101 tcatcaaata ctctccactg cattggctct ctgctgtcat tttccacatt tacaacaaat
    50161 ggatcaatgg ttttcaaatt ctcaggggta gtactttcca ctacaacata acttccatac
    50221 ggcagttccg ggctgactgc atatcctttt gtatcagtga tcagttctgg tactggtacg
    50281 gctgttccat tctcgtaagt aactgcaacc tgctctttgc tgaagtcata atttttgaaa
    50341 tcgcttgctg tatagctttc tccattggct ggcttcagtt ttccattttt tacctgtgtc
    50401 aggtcgctga tcagatatac cttaaatcct gctcctgcaa ccaggtctgt ttctgtctgc
    50461 tctccatctt cactgatttt gattaactgg aatgcctgtt tctttacctg ctcttttacc
    50521 gtaaggtctc ttgtcacttc gacaacatcc tgaccttcat aagttactga tacctcgtat
    50581 ttggtagtat ccagtgtata tccttccggt ggagtgatct cttttacata catttctcca
    50641 aggtataatt ctgaaaattc cagtgttccg tcatctccga tgactccctg tgcaatcagg
    50701 ctgtcctgtt tatacagaac ccctgttgtg ccatccggat gcacgatgtc ctcttttgca
    50761 tacagaccat acacagcacc acgaagaaca ctgtccccct gcgctttaaa tgcaagtgtt
    50821 tctttatcga tctttgcgat tttcaccttt ccggtgactc tctcatctgt tgctgttaca
    50881 gtcattgtct ttccggcagt aacagccact ttatatactt ctgtattcag tttgtatcct
    50941 gtcggagcag tgatctcttt cacgtacact gttttcagtg cagaatcaaa ataatcgctg
    51001 actgcttttc cgtctgttcc ggttgctgac attgtcatca gcagattttc acacttctta
    51061 tcagtataaa taccataaac tgctcctgac agtggattct gtgtattgac atctttcttc
    51121 ataagttcaa tgcgtgtcat attcagccac tgtacactga agcttaccgg cgctgctgtt
    51181 tcgctttcaa atacaccgat atcctgcttg gaatctccgg ttgtcaacac cagtgttctc
    51241 catgtctttc cgacagaacc atataagttg ccggaagcat aactgcctgt cagtaataaa
    51301 tctgcggaca gatagaatgt atctccacca taaatctgga tttttccatt tgtaacactt
    51361 gttcctttgg aaagattatg tgctgtcaca ttttctggta cactcagtgt cacatagttt
    51421 ctgtgatctc cagacagtgt gatatttggt gttttctgga tgtttccatc ccgtactgca
    51481 ttcagctttg tgcttgaaag gctcagttca ccttttggcg gttcttcctg tccaaacaga
    51541 taattgatat atgcgataac ccctgcattt acaaggtcat tataattaca ccctgtaaat
    51601 ccggcttctc ctgcataagc atagctggct gcaatgtgtg tatacacata cttttcatct
    51661 tccgtttttc cagacagata acttcctgtc agatctcctg ccccgccata accataataa
    51721 aggactttct gcagattttt attgctgtcc agtacctgtg ctacataact tccactcggt
    51781 ggggatgctt tctgggactg aaggcagtat gcaatttttc cattgactgt aaacaggcaa
    51841 gtaagataat ttccaatata acttggataa taaatagttc tcccctttgt caatgtaact
    51901 gtggaaccgc tgcttgtagc tctttcagcc gcagacaata ccatcacatt tccctcttcg
    51961 atgtcctctt tcagttcttt gattgcctgt gcagatgcat cttcttcgga tgtttcaatt
    52021 tctacatctg tgtagttctg cacaggcgaa tccgaatcat cttcagattc tgtatctgac
    52081 ttctgtttct ctccaccatt ttcttcggaa tgtgcctgtc cttcggtatc cgtcagagtc
    52141 actttacggg tgatggtata gctgtcactc ttatcttttg gaacgaccat ataagtggca
    52201 atatatgttc ctgctttgtc tgaatgatac tctccaccat tctcatcttt gatacttaca
    52261 agcgtaacat cttctttttc tgcatcataa tggattcctt ccatactggt ttccactgca
    52321 aatgtatcat ctgaaactgc ttttgtgata tcatctgctg taactgatgt atctttctgg
    52381 cttgttaccg cctgttcaga tgcctgtact tccgtctgct gttccgagcc tgtctgttcg
    52441 gccgcaaacg cattcgcact gctcaaggta ctgatgccca tcaggacagc cagtccaaaa
    52501 gcagcaattc tggtctttaa ttttctaacc ttcatgtgct tttccttcct attataaata
    52561 gattttttca gtaaaatacc gcagaagatg tttcctctgc ggtacgtttt atcttgcata
    52621 tcgatttttt tcttttttta cttcctgtct ggtacgtttt accgcttcca tgacagactc
    52681 cgttggaatc tggttatcct tacatccttc cagaattcca tccagataaa atttcgatgg
    52741 ctttgccgga acatctttgt gcggagcgtt catcatatat acaaacactt ctttctccac
    52801 accatcctga ttttttaccc gaatcgattc tttcccataa aaacttggga atccttcata
    52861 aaaatccaga ctcttctcac acgcttctgt gattttccat aagactcctt cgacatagct
    52921 tcctttttcc ggcagaaccg ttgctacccc attgcccgga gcccttcccc gaaatgccag
    52981 ccgataattt tcaagccgga cgttttccac aacttccgca gccggacacc tgtacttcat
    53041 ctgctccaga ttcatgttgc tgccataagc gaagtaatat ctatctttca ttttttctca
    53101 cctctatctg actccttctg tcctttgatt tttcttcaga cactcatacg tgcttttatc
    53161 atatctccac gcaagatcac catccagatt tgccagcaga tgttttcgaa catttttata
    53221 ttcgtctcca attaacccaa gtctcaataa aaatgtacga aatgtaaatg ccgggttttc
    53281 tgatatcagt gtctttttca tctgtgtcga tctctgattg attgcctgtg cggaaatggc
    53341 taatgccagt gtaatatttg ctctcacctt tcctgcatgt agtgtgcttt caaaacaacg
    53401 ccattccaaa gttcccttgg aaaacacagc atgaagattc agagcataat aacgggtatg
    53461 atcataatgg gtatgacttc catccctgcc tccataccag acatttctta ctcggtccat
    53521 tgtgattgat ttatttggta tttttcggat tttctccaat acctccggtc gcaccctctg
    53581 acaataacta tttgctcttc tctcctgcac tttcagtgct ttgaacagga tatcttcctt
    53641 tgcatacata attgtcagtg catttttcag actctgcggt gtatgattcg atgcatctac
    53701 atgaacatgc tgaccacagg actcatttac aaaacctcca tgctgtcgca gacatcgtac
    53761 tacttcctgt aactttccca tttcatcata ggataatttc ggtgatacca tttctgttga
    53821 atattctcca cctgcgtcta caattcgacc gtatactttc cgttgtgtat aaatgctccc
    53881 atcagacata aatttccact tttttccttc cagatctatc actgaccaga ctccataagt
    53941 agtgccgtca taatatgctt ctgttccaaa catttcagca acaacttctg cagcacgttg
    54001 tctggtaatc cctgtcattt cgatttctgt gccaaagtat tggtctttta ttccgatcat
    54061 gcctaatgga ttcctttctc agacactttc tgtgtgttaa gttcggagtc tgccttttgt
    54121 aacaatctgc caattgcctc gataacattg acagttactc cgtttccagc ctgtttataa
    54181 agctgtgcgt cagaagtgct gtctatgatc ttatcaatct gccagtcgta ataaccctgc
    54241 agtcgcagac attccctcgg aactaacctt cttattctgc cgtgatacaa gatgccatga
    54301 cgatcggttg cagtaattgt aaacatcggt tcttcgggtt ctttcatacg tctgccgttc
    54361 tgacgaacat tctcttttgc tggtgttaag actgctcttg ctccatcctc tgctaataca
    54421 ccagaacatt ctcctttgta tttgtggaca ccgcattgtc tggtattcag gcttctgcat
    54481 tcttccgtta ccaccggcgg aggtgaaaga tcgacaaagt gcagcgttcc ctgttgaaca
    54541 cctgttgtca gggtatgtgc gatctggtgt ccgactcttc ctctcctgct gttgagattc
    54601 gcataaccca gatcgatgct gtccccttct cttgcaagtt tgtagccgag ctttgtattc
    54661 tctttgattg gaacacctac atcatataat cctgtcttgc cgcccattcc acctgcctgt
    54721 gatgtcaggg tacagcttag tcctttggga ctgtagactc ttttcccctg acttcctgcc
    54781 cggacttgaa caagagctgc tccatttgtt tctggttcag gtaatacttt tccggcacat
    54841 cggaaatcaa aatatccgac aatatacacc cgttttcttg cttgggggac tccgaaatcc
    54901 ttgctgttaa gcactttcca ttcgacatgg taccccagtt cagaaagcgt actgaggatg
    54961 gtgcaaaacg tccggccttt gtcatgcgat aacaaaccgg gaacattttc aaggataaaa
    55021 tacgagggtc ttttagcttc aagaatccgg gcaatttcaa aaaagagagt tcctcttgca
    55081 tctgcaaatc cttctcgctt tccggcgata gagaatgcct ggcaaggaaa tcctgcacag
    55141 agtaaatcaa aatctggcat tcgttctggt tcgattgttc ttgcgtcatt acaataccac
    55201 tccccttccg tatcaaacag caatcggtag ttcttatccg cataagtatc cacctcgcaa
    55261 tgtccgacac agacaaagtt tcctgcccgt cttaagcctt cacgaaatcc cccgattccg
    55321 ctaaagagat caaaaaattg aatggttgcg gctatcaacc tcctttttgc tgataaaaac
    55381 aggcggcatg atctctcata ccgcccttcg ttattctgta tcaattggtt accattctgt
    55441 tggttcttct gctttggatt cttccggatc agtctctcct tctggttcag tttcctcttc
    55501 cggttcactt tccccttctg gtttgttttc ctctttcggt tcactttctc catccggttc
    55561 ggcttcctct tctggttcac tttccctttc cggctcagtt ctttcttccg gttgagtttc
    55621 ttctgttccc aattctccag ttgtctgttc ggattcttca ggctgctctt tgctttgcca
    55681 ttcgacctct accttttcca gtgcttcttc tataatctga attaagaagt ctttctgttt
    55741 catgtgtttc tttccgatga ctacttttaa tctctggaat aattcctctg atacctgtac
    55801 tgctactgtt cttactgctc ccatgcttat ttctcctttt ccttccactc tgttgaaatg
    55861 ttcttcaatg acctgttgta aaaatttttg tgtgctgctt tctgtgagtt caatctcttc
    55921 tcttactttc tcatgtaagt ccatcgggat ctttccgcag atatttttca tctctgccat
    55981 aacctttctc ctttcacttt tgctacacac aacatactcc aaagccgcat ggaaagctat
    56041 tcacaatacc caccaaagat atcgatgcat ttcttgcctt acccctagca gatccaacaa
    56101 tgtatgtatc gtgcaatcat aggcctcacc ccctttacaa attttccatt tgagatagaa
    56161 aatccatcat ctgatctggt atttcttccg atgatggttt cgttttcaat tgttccgtag
    56221 tgccacatat tcctaattcc cgaacacttt cttttatcgt cttttgaatg acttcttcca
    56281 tccatttccg atcctctttt tccagaattg cctgaaccag atactggctt ttctgccctt
    56341 ctggaacact ttgaaatttc tcccatgccc tctggtgttc tggatttttc aggttcgggc
    56401 ggaacgaata taccgggcgt gtcatctcgt ccacatctgc tctacaatcc gttcataccc
    56461 tgttgcgttg gcatggacgt cctctataaa aacggggcgg caaatcgcgt cctgtgcggt
    56521 cacgtggcgt tttaaaattg cggagcctcc ccccataaat acggtgggta ctgcccggag
    56581 atcaaacccg gattctgtca ccgcagacag tattctttct atatatttcc gtccgttttc
    56641 ctgtatgatc cgtctggcct cctctgccat actgcaagat tccctccgaa gcacacgctc
    56701 aatttgagtt tctgtcacag acagtccggt atttctacgc acttgttctg ctgtctcatc
    56761 aatacatcgg attactccaa gttccagact tctgcacgtt gctgcattcg gaacagcatt
    56821 gtccagcctc ataaggtcaa cggtccagcc accaatatca acaagcagaa cagacggttc
    56881 attcttcaga tactctggat gcagagcaag cgcagagtat ccctgtggga acagtttcac
    56941 atcttttatt gttatctcat ataactcact ttcatataaa aatcgcactg gctgctcttt
    57001 ccgcaacaga tactccctga atccctgttt ctctcttcca aaacttgaaa gcggaagtcc
    57061 aacagccaga attacctctg tctttcgttc cgctttccga tgcttgattt cttctgcaat
    57121 ggctgccaac gtcagcagat aataattatc attggacgtt ttatttttta ccagcgtctg
    57181 tcttcccgta ccgcagacat aatactttcc ttgatattgc agaacattct gcatggtata
    57241 cggctcgtaa tcatacccaa taattccact tgggaaagac acttgtcttg ttttaatggc
    57301 ataatagcca tgatcaattc ctatgattat cgcaatcaca tcctttcatt ttttctgttt
    57361 aacgtaaggt caaaaatgtt gtcactgctg caacggatcg ttgcaaaagt gccattcgtg
    57421 aacacttttc tatgttccag cgtcccaaaa cctgcggaaa atctggcttt tttataccac
    57481 tatcgccaga ggaacactac tgttccttcc cagccggtta taaaagatcc ctctcccggc
    57541 ggcggtccag cctgtccggg ggagggatat atcgcaagtt tcctttttga acaattgcct
    57601 gtctttttat ttttatgaga tgtttttcct gcttttgtaa aatgggaaga tactccggtt
    57661 ctgtcaaaaa cttccgtata taattgccct tttttcccag cttgttatca tttttcagca
    57721 gttctatttc taccatgtaa tgggtatctg gatgaaatct cttctcacac aaaatgctgt
    57781 ccccttttat ataatcagat ttagccgatt ctcttgctgc cgccaagagt tcataaatac
    57841 ttacttccac ctcttatcac ctcccaaaag accagaaaaa agcagacctc ttaatcgaag
    57901 tctgctcctg tcggatatgg taatcctctt tcttcatatt ttttatcgat ataatttcga
    57961 atccattccc tgtattcttc cgttaatgcc agttcctgaa attctaacaa ccacttttcc
    58021 ccttttgctt cgtcatcaag ccatcgatct tctatgcact ccggctcatc taacattttc
    58081 atcatcgctt caaatggctc ttttacatca tcaccaaatt ctttccataa ggcgattcca
    58141 tagttctcca gttcaaaaaa ttctgtcaga tatgtcaatc gttccatatc ccccaatgtc
    58201 attctttttt catctgacaa taatgctcga tctcttttgc cacatagatt taaaaatttt
    58261 ctcttttcca tctccacatc cattatactt ttacctcttc ctgttaaaaa taattcctta
    58321 ttacgaaaca ttataaaatt taaattcttt agaatcaata tatatgtttc tgtattacga
    58381 aactttttat tttataaggg gagttcatca ttatgcaaaa gattagacca gatatggata
    58441 ttggtaaaaa tatccaagca attcgatata aaaacaatat gactcaagac caagtagttg
    58501 caaaattaaa tcttatgggt atctccatca ccaaaagtac ctatgccaaa ctggaaacaa
    58561 atcgcatgaa cattaaggca tctgaattag ttgcacttgc aaaactcttt cataccgata
    58621 tcaatgcttt cttttccggc ttgctatagt tcgacattcc ccgactccct gtgtatataa
    58681 tagcatatta aaaataagaa ccagtctata ttcttatcgg atataaacca gttctttttt
    58741 cttatcaaat aatataaaag tgacagggaa ttcagattct tatcttctgt cactcatgta
    58801 attattattc tattttaaat ttaatgaatt tacccattct tgaatttctt tttcaatgtt
    58861 attttctata taaacgtcat acacattttt ccacattatc tgtgtatact gttattttca
    58921 accccataat tataacttgg tacacgctat acactactat aaactgtact ccattttatg
    58981 aaatccaccg ttccttcaat ccagcaatat tcataggtct gacgacaata ctcaggattg
    59041 gcagaagccg gtgtacaaaa aggagtacca tctttttgca aatcaggcaa cggacccgtg
    59101 cataaggtat cctgataacc agatagtcga cagtttttta aagatacgcg atctcctgaa
    59161 ttgaataaag caacagcctg tcctaccttt tctcctggac ctgctgtatt ttcgatgtga
    59221 agattttcta atacgacatt cgatccttct aagaaaaaag tagcacttcg gaaagtccct
    59281 aactggctac cgtcaagata ctcctgtttt gcaaaacgat taccggagat aactactgtt
    59341 cctgctccca caagtttaaa attggataat ctgctttcta tatattcatc atatcttcct
    59401 tcttttacta tcaagacctt tggatggcta tccggcaggc ctgccaaata atcgattcct
    59461 tcttggatag tttcaaaatc cgtcggggta tctatagcat tctttccaat tatcagtttg
    59521 tctttcatcc tcataaatgc tcccttatga attgtttcca aatatttctc atttcttgtg
    59581 tttccatatg accgcctgtt aaagaaaaag aagaaaagtt ttctggacta cccattgatt
    59641 gatacttttt gtccagccct tttctcagct tcagtacgcc ttcgtccaag cacatcgtat
    59701 cgttttttcc aactaagcta agacgagggc gcggagcgat tttttcttgg attgctaacg
    59761 ttgtataagc tgtcaataga tttggcacat agtaataaaa cccatgatga tctaggcggc
    59821 gatgtagcaa aagtatttcg atgtccacct gtgccgctag atcaatacaa actttgacac
    59881 gagaatccaa tgctgtcacc caccagctta ataagcctcc catagaaaga ccaatcgttg
    59941 cgagccgttg agtatctgtg tcttcccttg tctccaaata atcgatcaag ctgatcacat
    60001 cataaatacg cattccccat aacgtttttc ctgttaataa aaattgtttg aacaactcgc
    60061 tttcagaaat acctccgcgt tcttcaaatc cccaagcatc aatcgaccaa acagcatatc
    60121 ccattcctgt cagtacttct gcaaaagatg gagaagctaa gtaaggagca ccatggatca
    60181 attcactttt cccgactgaa aaatctccgc cgtgagaatg caggtaaagg accggcggaa
    60241 acggtccttc tttttctgga taagcataga gggctggtac tgtttccaat tcatttaagt
    60301 acaattgata gtattcaagc ataaaaccgt tttgtttttc tcggtataat cgttttccac
    60361 taggggtatg tctgctagat ggagcaccta aaaaatcatt caactgcata agcaaagttt
    60421 ttacgaacaa ccagttcatc catattttct gattgatttg attggaattt ctcaatactg
    60481 atttctttag tattttctaa tagaaatgct ggtccctcgt gattttcaat cgacatctgg
    60541 ttaaaggaaa ccttacccgc aaagccaata taaaatcctt gtctgctcat ttcctctatt
    60601 cctgtcatca tagcaggaac accgggaact gcatcttggg ccatcgagac atcgatattg
    60661 tcaaatgtga tttcttgtat gaattgttcc gctaatccat aaataaaacc tgcagaagca
    60721 tggacattcc ttgccgtgat attcgaaaaa tggatccttc tgaatgctgg tgtccgttca
    60781 tctatcggat aagccttttt ttcccataca tacggttctt tccctcgcgg accacagaaa
    60841 taataaagat tcagtataaa tggacacatc acattatcca tgactatatt ggatactcga
    60901 atatcttcaa cgatcccgcc tcgacctcgt ctagatttca atcgtattcc tcgatcagtt
    60961 tcttggaaga tacaatttga aatggtaata ttacgaatac taccactcat ttcacttcct
    61021 aatacgacac cgccatgtcc atggaccatc gtgcaatttg tgatcgtgat attttcgcaa
    61081 gcgatccttt cgtacgtatc ttcagtccct gccttgattg caatacagtc atctcccacg
    61141 tcaatatggc aattactaat acgcacattt ttgcatgatt ctggatcaat tccgtctgta
    61201 tttggtgaat ccgctgggtt caagatcgtc aagttatcaa acgttgcatt actgcaaaga
    61261 atcgggttga tcgtccaact tggtgactgg atcaatttaa tatctttcac agtaattcga
    61321 tgacagttat ggaaactcat caatttagga cgaggataag cgagattatc tggttcattt
    61381 ctaaaagtat gccaccattt ttttccattt ccatctaatg taccgaaacc agtgacagag
    61441 atgttctcta cattttgagc atagatacag gaagcataaa cttttcgatg gactccttcc
    61501 caacgtgaat gaacaacagg ataatctttt ggatcgtcag agaatttcag aaccgctcct
    61561 gctcttaaat gaagttcgac attgcttttt aaaaataaag ctcctgttag aaattcacct
    61621 gctggaacaa ctacggtccc gcctccgtca cttgctgcag catcaatggc ttgttgtatt
    61681 gcttcagtat tcaattcatc aattgatgcg ccaaatgtca aaatatcata catatttata
    61741 atgcctccac ttcaatcaat gcttgcaaga atgcaccaac gcctttttga tcattacaaa
    61801 taattggttc actgatgtag taagcatatg aaccatctcg ctgatctgct ccgcctaacc
    61861 ctgctacttg acagttttta ttcaagttca gccagccttc ttttgtttcc agaacaaatt
    61921 catcaagtaa tccttgatgt gatttcttca cttgtggaag ccattcttct ttgttcagca
    61981 gtcccaaacg tattccttta gccattgcgt aaacaaacat actactgccg gaagcttcca
    62041 gataatttcc tttccgtttt gtttctgttg ttacttgata ccatgtcccg ctgttttctt
    62101 cttgatatgg aagtaatgca gccagtgtct tttctagaat ttctttcagc aaagaacaat
    62161 ctgtttcttc atgaagtaat tcataggtat ctgctgcagc catcaaatac cagccaatcg
    62221 atcggctcca aaaatgatgg gatagacctg tttttggatc agcccatggt tggaccttct
    62281 tttcatccca tgcatgaatc agcaaatttg tttttggatc gaatagatgt ttataactga
    62341 tttcaaactg tctcaaaaca tcttggtaat ctttttcttc actaaaattc tggataaatt
    62401 cagcataaaa aggagaaccc atatataagc catccaacca aatttgataa ggataaattt
    62461 ctttatgcca aaaagctcct tcggatgtgc gtggatggtt tgccaactgc tgggaaagga
    62521 gttctgctgc ttttttatat ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct
    62581 ttcctgtatt tacatggtcg atattgtatt cttcaacgct gtagccacgg atcgttccat
    62641 cttcttgcac gaaatagtcg atattgtctt ttatgaaatc aaaataaatt ttttcttggg
    62701 ttcgttccca aagtaattgg aaccctttaa gtacgacgcc atagtcataa gaccattttc
    62761 ctttatacca cttttcttca tataagcgag gtgttctttc catgatactt tttgctaatt
    62821 tctttgacca gttttccatt atttgctctc ctttaaaaat taaaatagga tttgggacaa
    62881 ataccaagaa gtatttgtcc caatcgtcta tattactgtg ccgctgcttt gtatgcttcc
    62941 tcgtactctt gtgtgattcg atcgcctcct tgtgctttcc agtcttctac agcttttttg
    63001 aatccatctt cgtcaagctg tcccatgatg tattgcaccg tagcatcaat gacgattttt
    63061 tcaatttctg atcctacttc attgttcgtc ggtgaatcca atggcacaga aggatccatt
    63121 acagcaaatt cttcattttc tttgatcaat tgattagaga attctttttc agggtctgta
    63181 tctttgaaag agaacgtcac ttcactagga cgagagcttg ataacggttg cacctcttgt
    63241 tgccaaagat ccatatcgat aaattcgata gaaccatcat cattcaattc ataatgcgtt
    63301 ccttcgatac cgccagtcat caatttatac acatcttctt ctaaaagatc atcaacaaat
    63361 tgaagtaatt tcttcaattg tgcttcgtct tttacttgag atttgggaaa tgctagcaag
    63421 ccgccaatac catttccttc tgaccaaaca tgataaccgc cgccttcacc atctgagatc
    63481 ttgtttacag gcactaattc catatcatct tgaatacctt gacccatcgt cttcagattc
    63541 ttgatatcca ccattccagt gtagattcca gttcttcctt gtgcaaattg ttcttgctga
    63601 tcagtctttt gggtaactgc aaagtcttgt gccaaatagc cattctcaaa tagatctctt
    63661 gaaaaattca aagctttcat atactcttct gtatcaaatt ctggagtgaa tttgccatta
    63721 tcgtctacag cccagccatt tggtgctcca aaataagaag tgaaaagttt aaagctggta
    63781 taacgaatat cactaggcgc acgatcgcca aaaccaactg tgtcgtccac accatttcca
    63841 tcaggatcgt tttctgtaaa ttgtttagct acttcataaa gctcatccat ggtagttgga
    63901 acatctagtc ctagattatc caaccagtct tttcgaatga ctaagccagc acgagcgtag
    63961 tctttttgta tcggtacgcc gtacaaggca ccgtcgatac tcgcagcttc gattctatct
    64021 tcagaaattt gacttaaatt tttatagtct tttaaatatt ctgatacatc ccagaatacg
    64081 cctgatttta acgcattgcg gacagaagag tttttcaaca tcgcaatttg aagtgtaacg
    64141 atatctccta gttcatcaga agccaatgca gttgttaatc tttcttcttt tgaagcatca
    64201 ggaatccagt tgaatgtcag ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg
    64261 tctgtaggcg gtgaagcatg atgcaaaata tttaaccaag tgattttagc ttttccatct
    64321 tctgtctctg aagaactgtt acttgaactg ctgccgcatg cggcaagtgc tagaattcct
    64381 gcaaaactaa gacctaattt tacgagcgtt gcttttttca taaattttct ccttcactgt
    64441 tttaataata tttttgtttt cttctgtttt ttctgtatat gagttcttcc attccaaatg
    64501 gcaaaatata gaatttgctg tccacgtgac tgcggaaaag aagagactgc caccgataaa
    64561 ggttaaaaca cctggcattg tttggatgat catcagataa atccctacca tacttaatat
    64621 caacactaat gagtaatgaa gatgtgagaa tccgaataaa aatgccattt tcattttgaa
    64681 aaaatatcct ttccaatcat aatgagccat gattgggaaa atatagagtg ctgagagcac
    64741 atatatgaac gcagcaatat acaaagcgat cctaagatac caattgtgta tgaaagaaag
    64801 atccacaagt aaaacaatcc cgatggcgcc atatatccag ctcatcagca aggtttcacg
    64861 atagttttct ttaaaatatg tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg
    64921 cagccatttt ctaatcactg caaccatcgc atacgatgca ggaccgaaag taaaaactcc
    64981 caaacctgcg atgactaaac cgccccacag aaaattcaaa taaaccaact gtaaaatacg
    65041 atttagcctt ccgtttattt tatctaatga ctgcagcata tctttccctc tctttctatc
    65101 ttagtaaacg ccgtcttctc ctagcttttt ggcaatctta ttagctaaaa ttaccataaa
    65161 caatcctacg acccctttaa acaatcccat tgctgtactg aaacttaact gtccattctt
    65221 caatccagct gtataaatat aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt
    65281 caataacaag tacatatgct cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag
    65341 caaggtaatg attactggac gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg
    65401 tcctgctcca tccatttctg cagcttcata caactgtgta tcgactgcag ttatcgctga
    65461 aaggtagatg atcgtagacc agcccaactc tttccagatc acttgtccga tatatactgt
    65521 gcgtgtccat tcaggggatg ttagaaaact gattttttca aatcccattg ttgcaaggaa
    65581 ttcattcaat acccctccgt caacattcaa aaatacataa gtaatcgaaa caatgattac
    65641 ccaagacatg aagtgaggga tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt
    65701 cgttacttca tttaatagta aagacaaaat gattggcatc ggaaaaaaca cgaagatatt
    65761 taatccgaac aagatcaacg tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa
    65821 acgaatgaaa tgtttgaaac cgacccaagg acttcccatt attcctaaaa atggttgata
    65881 atcttgaaaa gcaataacga gtccccccat aggaagatac ctaaaaatca agaaataaag
    65941 gactcccggc agaatcatca catataaaaa tttattcgtt ttgattcctg caattatttt
    66001 ttttcgtctt ctgagctttt cttctttttt cgatagcgtc agttgaaatg gttcttgttt
    66061 ctttacactt tccataagcc cctccttagt tttgtgaatg tttgctgctt atgacttcat
    66121 gatagataac tatctgaatt acgtatataa tcatttttac cgtaagtaaa aacgcttaca
    66181 tagcaatgat ttttgtgcta tacaaaacga taaagtgtat tttttcacaa ataaactacg
    66241 catagcaaac cttctatttt tttctatctg acacttagca taggaaatga ttatatacgt
    66301 aatctttcac cgatgataaa ttgaacatat agaaacagaa acatttaatt tattttgagg
    66361 aggagcctat ctatatgaaa aagaaagtct ctataggaga aagaagtttt acacttttca
    66421 acaatacgtt tctaattttg ctagctttga tctgtatcgt tcctttttta aatatcatcg
    66481 ctacatcttt tgcttctaca caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta
    66541 ccttttcttt agacgcgtat cgctacattc tttctacgcc gaccattttt agagcacttg
    66601 ctgtttcgat tggtgttaca ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga
    66661 tggcctacgg tctttccaga aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg
    66721 tattttctat gttgttcagc ggggggatga ttccaacgtt cttagttgtt cgttctctag
    66781 ggctggtcaa ttcttactgg gccatgatcc tgcctgtggc tgttaatgca atgaatatga
    66841 tcattatgag aaactttttc caagccttgc cggatagttt ggaagaatca gcaaaaatgg
    66901 atggttgcac agattttggt gtatttttca aaattatgct gccattagcg ttaccgtcta
    66961 ttgctactat ctcgcttttt tatgctgtta cttactggaa cacttatatg actgctattt
    67021 tgtatatcaa cgattcatct aaatggccga tacagattct tttacgccaa atcgtcattg
    67081 tatcaagtgg gatgcaagca gaaagtagtg ccgttgatgt gattccacca gcacaaacag
    67141 ttaaaatggc tgttatcgtt attgcaacag tgcctatgct gattgtttat ccttttgttc
    67201 agaaatattt tgtcaaagga gcactcgtag gttctgtcaa agggtgatta tgtactgaac
    67261 gcaaaatggt agttgtgtca gaagtggtca gcttcaagaa ataagaaggc atttacgaaa
    67321 attgcttctt aaatttttgt gaaatttcgg cttatttttc gaagaatcta ctcctggaac
    67381 accgtgtatc agaggttgtg aaagagacgt tcagccttga gcgatgataa aaactctcac
    67441 aaacattgcg acgagtaacc cacaggagca ataagaagcg attttgaaaa atagcttccc
    67501 atatttcaca aaattatgcc ttattgtcga tagcctgtct cttgaacgcc atttattcgt
    67561 aaaaaagaac tgtgacaaga cttttgtcac agttcttttt gcgtgcttag ccttgaaatt
    67621 taactatcca taaatacaaa tactagggtt attctaactt cttttattgc ttgaatcagt
    67681 ccacaagaaa aacagcctct tgattcacaa atattttttt cgatattctc ccggcgtaat
    67741 cgattccttc tttttaaaga aacgaataaa attttgcgga tttcgatatt gcaatctttc
    67801 tgcaatctct tttacggaga ggttagtctc tttcaaccat ttttttgcta cctctaaacg
    67861 atgattttga acaaaatcac caaatttttc tcctgtttcc tttttaaata cattacttaa
    67921 atagttaggg ttataatgca acctatcagc gatcacatct aaagacagat cacgatcata
    67981 ttctgtgtag acgatatgca taatattctc cgacaaattt ttaaattcat gatcagtgat
    68041 tgcttgagag cattctgtga ttggcagaac taattgttct aaaacaagtt tttctagttc
    68101 tttgggatga taattgttca gtgcttttac ataaatttgt ttcatgttat caaagttttt
    68161 tgaatctact cccaataact gtcctaattg aacgagttca ttgatcaagc ggaccgaaac
    68221 tacttcccga ctcaaaggat ttttattttg tgcaaacaat gcatcaatca attcatgtac
    68281 taaattcttc acttcctgat ttccagaacg aatggcttca aacaatcgat tttgttcttc
    68341 tatcggatat ctaataagtg ttttttcatg agcagcagga acgatttcct gataaaaaat
    68401 aattgaactt ggaccagtat ttacacgata atacagggct tcttttgccc gatcaaccga
    68461 ttgcttgctt tcattcagcg tttcgaaccg gttactgatg cctatactga tagttaaatt
    68521 caagtattct tttacacgtt tttgaatgag tcggcaatac tccatgactt ttttgcttgc
    68581 ttctgcatca tctttatcca ttcgataaat agtggcttgc atttcttcat tcaaaacgat
    68641 cggcagcatt cgattttcct ttggcacaat ttccgtgatc atgttgttga tcgctaaaag
    68701 tagcaaatca cgctccccac cgtgactatc ttctaacaga tcgatttgaa ccaaagctgt
    68761 ataatagcag tcattttgag aactataacc aaattgctgc aaccgctgat ctagttctcg
    68821 cctttctact cgatttctaa ataaactaag tacaaatagc gtttccagct gtggcttttg
    68881 ggtaactaag tgtgcactta acgcttcttt ttctcctatg atccgactga ctgattgagc
    68941 gaccaaatct agttctttcc cactgaatcc ttttgattta agattcagac gttcctgtat
    69001 ttgagaaatc ggacgggcaa atctgtccga aaataagtag gaaagaagac caatgagtcc
    69061 taccagtgtt aaagacactg caactagccc gattctcaga ttgcggatca ttgcgccaat
    69121 tgcactagag tcaacttcta atctgtaaat ccaattatta taatctgatt tcagtactat
    69181 atacgtttta tctttttctc ttatcacttt cagctgattg taatctagat ccacgtccgt
    69241 taatacagtt ggttgctctt tattttttac tgtctgagta gtaaaaaggt tgccaacttc
    69301 gttttcaata gaaagtatgt cttctccact ttcaccaaca acttgttcta ttgtatgttt
    69361 ctctatcgta gcaattccca atgcatattt ttctttttca aacattggca aagaaatcac
    69421 catttcgatt cctttgtttt tcttttgcca aaataaatta tttttgttat ttatgtactt
    69481 ctgttgatac ttttcaattt cctcttcggt cagttgactc aacgatccat tgattactcc
    69541 ccatttacca gccaaactga ctaaatcata attattatca tccataacaa tcgtttctat
    69601 aaaatttagt tctttcctga tttcagaata agtggagtaa tctgtatatg tcagcggatg
    69661 cttgaatatg tccgagaaat ttttggtcga accatacgta gaaaacgaat attcaatcgt
    69721 tctaattttc cattcaacgt tagttttaac ttgcctgagc aaattctttt tgtctgttaa
    69781 ataactcgat tcgatcgatt cataggtatt ctggtaaatg aataaactaa atcctatgac
    69841 aagtacggct cctaataaaa acatcggtaa aaatatttta taaaatactg tacttttctt
    69901 cattttcttc ctctcctcct tttaaaataa aaggctttca tttttctgta aaattgatac
    69961 tcatctaatt aaatgagcat tccccgcttt caagaatagc ataattaaaa ataaaaatcc
    70021 aagtaatttt taacgtgtgc aatcttttca tatcaaagat ctattgactt atcgtagtta
    70081 tcgtaatatg atgaatgcgt taacaaaaag tgaaaaagta aggaaaatga tggaatggta
    70141 acgatcaaag atgtcgctaa agcagctggc gtttctcata cgacagtttc tcgtgctttg
    70201 aataacagca gtttaataaa aaatgaaaca aaacaaaaaa tacgacaact agctaaggaa
    70261 atgaattatt cgccaaacta tagtgcaaaa ggtttagtga atcaaaaaaa atatctaatc
    70321 ggactatttt tctcaagttt gcataaagga acttcatcaa atttcctagc agattccatt
    70381 gcaggcatcc attcagtatt agacacaaat tacagtttat ctgttgaaag tatcgatgtt
    70441 gttgatttaa cagaaatcaa cctgcaacgc tacgatggac tgatcgttat gagtcaaagt
    70501 gatgatgatc agcctttcat cgactatctc aaagaaaaag aatttccttt cgttgttgtc
    70561 aatcgtcaca tcaatgatcc agcggttatc aacgtcactg ccgatgatgc ggccggtgtt
    70621 atgcaggcag tagattacgg tattggatgc ggacatacga agatcgctta tattggcgga
    70681 aagaaaaatt ttcgttcgac aaaagaacgg gaaaaagggg tattgaaaag tctaaaaaaa
    70741 gcgagcatcc ctgtcaatcc agcctatttt ttaacaggag attacagctt agaaagcggc
    70801 ctccaaaaca tggaatacct attatcactt tctgacctgc ctactctggt tttttgcggc
    70861 aacgacgaca tagcgattgg tgcattgaga gctgtacatt tagctggcct ccgtgttccc
    70921 cagaacattt ctctgatcgg gttcgatgat tctccagtcg tctcctatct caatccgccg
    70981 cttaccaccg tacgtaagcc tttgaaagaa atgtgtcaag aaggaacaaa gcttttacta
    71041 gatttgatcg aaggcaggac gatcgaacaa tctaaaattg agctctccac cttgttgatc
    71101 cttcgtgagt cgatcaatta cttgtaaact acaagtaatt ttatataaaa atgttaacgt
    71161 gtgcataaca cagaaaggaa gtttatatga aaaaactgac aaaagaatta gtgaaaaaaa
    71221 acgaagcacc tgtaaaagtt cttcagtttg gtgaaggcaa ttttatgcgt ggatttattg
    71281 attggcagat tcagcagctg aataaccaac aattgtttaa aggtaatgtt gcaatcattc
    71341 aacctttaga acaaggtctt ggaaatatga tgaaccagca agataattta tatacggtca
    71401 ttttacaagg actcagcaat ggcgaaatcg ttgatacttc cgagattatc accgtagtag
    71461 aacaaatgat caatccttat gatgaatggg atgcttatct atctctagca gaaaatgacg
    71521 acttggccta tatcgtctcc aatacaacag aggcaggaat ccaattcgtg gagactgata
    71581 atttaacaga tactccccca cagagcttcc cgggcaaact gacagccttt ttataccgac
    71641 gtttcaaatt aaacaaagct ggctttacca tcattccttg cgaactgatt gacagaaacg
    71701 gcgaacgtct aaaagaaatc attttacgct acgcagaaaa atgggattta gaacctgctt
    71761 ttgttgagtg gttagaaaaa gaaaatgttt tctgttgtag tttagttgat cgcatcgtcc
    71821 caggttatcc tcgcgaagat gcaggtgttt tcaatcaacg gcataactat gaagatcaat
    71881 tgatggtcaa agcagaacca ttcatgcttt gggtgatcga agggcctaaa gaattggaga
    71941 atcaactacc attcaaacag gctggcttga atgtcatcat cacagatgat atgactcctt
    72001 atcgtgaacg taaggtgcat ttgcttaatg gaccgcatac agcgatggtc cctcttgctt
    72061 tgttggcgga attggaaact gtagaagaag taatgaaaga tcctttattc tccgcatatg
    72121 ttgatcagtt attcaatcta gaattgattc ctatgctttc tttacctaaa gatgaattag
    72181 ccatttatgc tgaccaaatc aaagaacgtt tcttgaatcc ttttgcccat cacaaattag
    72241 aagcgatttc attaaatagt gtttcaaagt tcagcacccg tctgcttcca gtgttcaaga
    72301 agtatatcga agaacaaaat caggtaccac cgttaatcac tgtttcacta gctgcgctac
    72361 ttcttatgta tcgaggcgat caagtaaagc cacatgatga tgaaaagacg ataagcgaat
    72421 ttactgatgc ttggtctgat aatggaacag ctattcctcg attattgcaa aacgctgcgc
    72481 tttggggaga agatttgtct caaataccga atgtgacaga tacagttcaa gaaatagctg
    72541 caaagatcga tcaaaaaggt gtccggtcag tattacaaga aatgaaagga acgatggtcc
    72601 atgcttgaaa ccctgcaacc aacaatgaaa ttaaatcgac gagacagtgt ggtcgtcgca
    72661 ttaaccccta ttccaaaaca aacacaactg acgattgatc agcaaacgat caccactctt
    72721 gaagatatcc cgcaaggtca taagattgca ttgggtgatt tgaaagctgg agacaacgtg
    72781 atcaaatatg gctatccaat cggtcatgtg acgacagacg taactgccgg tcaatggctg
    72841 cacactcata atgtaaaaac caatctttct ggtgaattgg attatcgata tgaaaaagat
    72901 gtccatccaa gtcattacct ttttgagaac cgaacatttc aaggttactt gcgaaaaaat
    72961 ggaaaagtag ggattcgtaa tgatttgttt ttagtaccta ccgttgggtg tgtgaatggc
    73021 attgctgaac tgatcgtcaa acagttcaag gaaaaacatc cagatctggg acagtttgac
    73081 catattacga ttttaaagca tccttatggc tgttcgcagc ttggaaaaga tcatcaaaat
    73141 actcgagaaa ttttagcgga cgcagtaaat catcccaatg ccggaggagt tttagtattt
    73201 ggattaggtt gtgagaacaa tacagttcct gaattcaaaa aaattttagg tgaatatgat
    73261 gaagaacgag tgaaatttct agtggctcaa gatgtttacg atgagatcga acaaggtgtg
    73321 gctctattag aagagctttt aacagcggcg gaaaatgacc agcgcatatc agtcccgctc
    73381 tctaagttga atgtcggttt gaaatgcggc ggttctgatg gtctatcggg aataactgct
    73441 aatcctttac ttggtgcatt ttctgactac ttgattgctc aaggaggcag cacgatctta
    73501 acagaagtgc cagaaatgtt tggcgcagaa caagtattga tggcacgagc ggaaaatgaa
    73561 gaagtttttg actcaatcgt tgacttgatc aatgacttca aacagtattt cttgtcttac
    73621 ggggaaccag tttacgaaaa cccatctcct ggtaataaag caggtgggat cactacatta
    73681 gaagataagt ctttaggctg cacgcaaaaa tcaggaagct ctcctgtcgt tgatgtgcta
    73741 caatatggcg aaaaaatcag aaaaccaggg cttagtttat tgcaagctcc cgggaatgat
    73801 cttgtagctg cctctgctct tgcttcatcc gattgccagt tggtcttgtt taccacagga
    73861 agagggactc cgtttggctc atatgtccca acggtcaagg tatctacgaa tacaactctt
    73921 tttgaacgca aaggtcattg gatggatttt aatgcaggcg tcttattaga acaaccaatg
    73981 gaagtaatct tagaatcgtt tgttcaaaaa atcatcgctg tagctagtgg agaagaaacg
    74041 aaaaatgaac aaaatgacgt tcgagaaatt gccattttta aaaacggagt aacactttga
    74101 ttttcataaa acagtataaa aagaaagaag gaagatttat gtttttatct gatgattttt
    74161 tattaaaaga cgattgggcg aaaaagcttt accattccta cgcaaaaaac atgcccatta
    74221 ttgattatca ctgtcactta gatccaaaag aaatttacga aaacaacaat ttcagcaatc
    74281 tgacagaagc ttggttagga ggcgatcatt acaaatggcg cttgatgcga gcttgcggcg
    74341 tgcctgaaga aaaaatcaca ggaaatgctt ctgattttga aaaattttta gcttggtgtc
    74401 aaaccgtacc aaaaattatc ggaaatccgc tttacagttg gtcacatctt gaattgaagc
    74461 gtttcttcgg tattgacttg ttgataaatg aagaaaatgc agagaaaatt tggacacaag
    74521 caaatgaaat tttggcagct cctgattttc gccgtcggga aatcgcaaaa aaatcgaatg
    74581 tagaagtgat ttgtacaaca gatgatcccg cagacgactt gcactatcat cagttgcttg
    74641 ctcatgaaga accagatttg aaggttctac cttcattccg tccagataaa gcactcaata
    74701 ttgaaaaaga aggatttaat gcatgggtca cactactgga agaagcagct gaccaaacga
    74761 tcaccaccta tcaagaactg atagaagtct tagggcagcg gatagaatac ttccatcaac
    74821 atggatgtcg tgtttccgac cacgctttag acatcttacg ctataaagaa gcggatgaaa
    74881 cagtattaga agagattttt caaaaagcac gagctaacaa actattatct tcagatgaaa
    74941 tcgatgctta tcgtacagaa acactgatac atttgaccca cttctatcat tctcataatt
    75001 ggacgatgca gctgcacatc catgcttatc gtaactgtaa tacacaaatg ttggaaaaat
    75061 tagggcccga taccggctat gatggaatga atgatctttc tttgactatt cctctgcaaa
    75121 aattattgaa ccgtgcagaa gaaacggatc agttgccaaa gacgatcctt tattcattga
    75181 atccgaatga ctatcctgcg attttagctt tgatgggctg tttccaaaaa gaacagaatg
    75241 gaaaactgca gttaggttct ggatggtggt acaacgatac tcgagctggt atgcgcgatc
    75301 aattgacaca atttgctgat ggtagtgctt tggggaattt tgtcggcatg ctgacagatt
    75361 caagaagttt cctgtcctac actagacatg aatatttccg tcgtgttctt tgtgaatttt
    75421 taggcgaaat ggtcatgcga ggagaagcac ctgaagatga acaactttta ggaagtctag
    75481 ttcaagcgat tagttataca aatgctaaaa attactttgg cttttttgcc taattgatta
    75541 atagtaacag taacgaatca aaaacaggat agtgaaataa cggaatccgt ttatccacgt
    75601 tttagagaag aagacggacc aaatttgctc cgtcttcttc tcttgctgtt ctgcttcatt
    75661 gcctataaac ctttatgaaa gaagagataa catgcctact atccatattg ccggtgattc
    75721 tactgcggca gaaaaagatg aaaacaagcg tcccgagaca ggttggggag aaaaaatctc
    75781 tgcttatttt tcacccgaga tccagatcaa taaccaagca aagaatggac gcagcaccaa
    75841 atcatttatc caggaaggaa ggttagcccg tctagcttca cagtttcagc cgaatgattt
    75901 cttatttgta caatttggac ataacgacca gaaaatccaa gaagaaagag gtacgcttcc
    75961 cttcggtagc tatttaaaaa atctgcagat attttttgaa tgcgcaaaaa aagcgaacgt
    76021 ccaaatggtc ttactgacat ctgtgacgcg acgagattac ttagaaaacg gaacattgaa
    76081 tccagatata cttggagact atccaaaagc gatgcgtgca tttgctgaga aacaccatat
    76141 tcctctttta gatgtgttct caagatcaca agaattgttc cagaccttct caaaagaaga
    76201 gacaaaaaaa ttctacctgc atcttatacc tgatacttgt aaaaattacc cagaaggact
    76261 aaaagacaat acccacttta gtccagaagg cgctgataaa gttgcccgaa taattgttga
    76321 attgataaaa gaatcccgtc tgcctcttgc taattattta caaaaaggag tttgagatgt
    76381 ttccattaaa tttaggcatc cgtgcccacg atttagatgt tcgaaatcgt gaagaaatcg
    76441 ttcaaaaagt atcacagctt cacttgtccc atatccaatt tgcaccacaa aaagcatttc
    76501 ctgaacaact taaattaact gacatgacac ttgggagtgc tgcctatttt ggtgaatact
    76561 tccaaaaaaa caatatcgaa ctttccattt taggatgcta tatcaaccta tccagcaaaa
    76621 atcaagaagt tcgtgcacaa gcattggcaa ctttcaaaca gcaaattgct ctgtgctctg
    76681 cctatcaagc caagatgatt gctacggaaa ccggtagtgt ctctgtcggt tacacaaaag
    76741 aaaattttac tgaagaagct tatcagatcg ccagaaattc tatcattgaa ttagtcgctc
    76801 atgcagaaaa ttttggcgta actgttgctg ttgaagcagg gatcaatcat ccaatctata
    76861 actatcagtt agctaaacgt ttaatcgatg aagtagcttc accaaattta aaaatcattt
    76921 tagattgtgc gaatttgatg cataaagaaa atcatgctga tcaggaaaaa gtgatccgag
    76981 gtgccttgga caatttacat ggttatatta cagcgcttca tctcaaagac tatatcatgg
    77041 aaaatggaaa gatccgcatg gtacctgttg gcaagggctg tttagatttc cgcccggtac
    77101 tgcattatat caaatatgag cgtcctctaa tgtacgccac tcttgaagct actcctgaga
    77161 tttatgttcc tgaagcaata gaacacttgc agtttattta ccaagattgt taaggagaag
    77221 attatggagg aacgaataca gaaaagttat caaaaatatc atgcaaatgc ttttcagaca
    77281 ttgctgcatc tttatcaggg aaattattgg cgttttgcca tgtcaggact cttttttatt
    77341 atcaaacatc ttccatcttg gttaatgccg atcgctattg ccaatgtaat caatgcagct
    77401 tcatcgaaag attcttctca gttgcaggcc atttatttca acgctttttt gatggcttgt
    77461 ctgattggtc aaaatatatt gacgaattat tttcatgtaa aataccatag cacttcgatc
    77521 cgtcaagtag aagctggact aagagcgacc cttatcaaga aaatccaaga actatctatt
    77581 ccttatcaaa aagaattgca atctggacga atccagtcaa agatcatccg tgatgtagaa
    77641 gctatccaaa cgttgtcctc tcaagtattt gtcagcggat taaatatcgc tgtcaattta
    77701 ttcgtagctt taggtatcac gctttataaa agtcctgttg ttttcatgtt tttcttattg
    77761 actgttcctc tcgccgcatt cgttactctc tttttccgta aaaggatcag acagtccaac
    77821 tcagacttca ggcaagaaat ggaagagact tctgcaaaag tgatcgaagt gatcgaatta
    77881 gcaccgattg ctcgagctca tgggttagaa aaacacgaaa caactcgctt aaataagcat
    77941 tttactcaaa tctatgaaaa aggattccac ttagatatcc tgcaatctgt tttcggatca
    78001 gtcagctggg ctagttttca gatatttcag ctgatttgtt tgattttcac tgggatatta
    78061 gctttacaag ggcgcatcca acccggcgat gtcgtgatgt atcagacgta tttcacgacg
    78121 atcgtcaatt ccgtttctgg aatgatcacg ctgattccta cgatttcaaa aggcatggaa
    78181 tctatcactt ctattggcga actgttgatt gctggcgatg tagaaactta tcacggtaag
    78241 aaaaaaatcc aacatttgga tggaaatttt cgttttgagg atgtttcttt tctttatccg
    78301 gactcactca aaccgattat cgatcatttt tctctagagg tgaaagctgg agaaacaatt
    78361 gcttttgttg ggccatctgg ctctggaaaa tctacactcc ttaacttatt gattggattt
    78421 atccagccga cttcaggaaa aatttttcta gatgatcagc ctttggatga actggatatg
    78481 cgaagtgtgc gaaattattt agccgttgtt cctcaaacca ctttgttgtt ctccgcttcg
    78541 atcaaagaga acatcactta tggcttgaaa aacgtatcaa aagaacgttt agatgaagtc
    78601 atcgaagctg cgcaactttc ttcactgatt gaccagcttc ctgatggtct ggatacctta
    78661 gtaggcgaac atggcaacaa gctgtctggt ggccaaaagc aacgtatctc cattgcacga
    78721 gcgttgatac gtcagccaaa aatcattttg ctagatgaag caacctcagc tttagataac
    78781 cagtcagaga aaaaaatcca gcaagccctg aattacctga cagaaacacc cactactttt
    78841 attgtggctc atcggctttc aacgatcaaa gaggcagata aaatcgttgt gatcgatgaa
    78901 ggaaaaatcg ttgaaatagg tacttacgaa aaactgttag ctcaaaaagg ctatttctac
    78961 cgcttgaaaa atgaatgaca aaaaatagga gctatgacaa gacttttgtc gtagctccta
    79021 tttctgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tagtcatcaa
    79081 acatgagaag ctgttttttg aaattgttcc ttgtaactca gagttaaacg gtaggctcac
    79141 gacctcttgg taaatggtgt tcaagagaca gcctatcggc aataagaagc aattttgtca
    79201 tcgctcaagg ccgaacgtct ctttcacacc ctctgttgtt tcataaaata gttttattcg
    79261 ctgtaaaggt cgcgaactgg tgtcatgatg atacggttca agtcattgat gattgtactg
    79321 aagccttgct ctttttgcat caagtcattg attacttctt ctgattggat tttcacagcc
    79381 atttcttgtg ctttcattgc atcttcgtcg ctgaactctt gtccactcat ttgtttttct
    79441 tgtaattcga tttgcatcgc ttggaagtct ttgaacagtt tgtgtgcttc ttcgtttgct
    79501 tgtactttct cgtacgcttc tttcaacgct ttgaattctg gtaattcacg gatctcacgt
    79561 tcgatttgat tcgcgctatc gtagatgttt gacatgttat gccctctttt cttttagtag
    79621 ttactctcta ttgtaacatg gggttattcg ttttcaaagg ggctactctc cttgatattt
    79681 ccgccagagg tctttagccg cttcaccaat tccctgaacg ccttctttca ccatatcgcc
    79741 gaagtttttc agcccttctt ttgcttgttc accatattga ttcaagttat cttgccaatt
    79801 tccagactct ccattagaat ttcctgtgtt tccaacttga tctgcagcta ccacttgtcc
    79861 tcctgtggca taggcatctg ctacactaaa tcctgttttt gaagtatacg gaaggatgcc
    79921 ttctgccgct gatttgaaga ctttcccgac aacatttcca cttgtccctt ccaaatagtg
    79981 cagcttgctg gattcttcgt aacccatcca tgtcgaaatc actacgtctg gcgtgtaacc
    80041 gatcatccat tggtcatttg cttttgtggc atcgaaatta gtttctgtgg ttcctgtttt
    80101 ccctgctatc gtataacctg caggattcgc agcagccgct gttccatttg aaaatgtacc
    80161 aagaagcatg ctggtcattt catcggctgt ttcctttgat atgacctgtt ttggtttttt
    80221 gtttgtgtta tcgacgatca ccgctccagt cgaatcaacg attttcgtga taagatgcgg
    80281 cgtatacatc tgaccatcat tggcaaatgc actgtaagca cccgccatga tcaatggaga
    80341 tactcctttt tccagtccac caagggctaa gccataatac ttatcagatt ttgctaaagg
    80401 caggccgaat tcttctgctt tatcatatcc tttttgtaaa ccgatctcat gcaaaagcca
    80461 tacagtaggc aaattcaaac tttgtgcaac agcttgatac atcggtactt ctccttggta
    80521 tgtcccgtca aaattatgcg catcgtaata actttgcggt tcgtctttca agatactgga
    80581 aggcttgtat ccagcttcca gtgctggtgt atatacgctt aatggtttga tcgttgatcc
    80641 tggtgaacga cgcatctgtg tagcaaagct gaatcctcta aagacatgat ctcctcttcg
    80701 tcctactagt gcttgtacac cacctgtatt cggatctaaa gctactgaag cagattggac
    80761 catggcacca tctgaagcat ttgctgggaa taatgcactg ttttgataag tagcttctaa
    80821 ttgcttttga taatcctgat ctaaagctgt atagatttta tagcctttat tcatgacatc
    80881 ttcttcatca agaccatact cgttaactgc ttcatcgatc actgcatcaa aataataagg
    80941 ataacgataa ccagaatctg aattatcgta tgtgtcatta agatacgcgc tgatatctgt
    81001 ctggctttct gcttgttctt gttcttttgt gatttttccg ttttcttcca ttacacttag
    81061 tactgtgttt ttccgatcat tcgcattttt tgggtgatcg atcggattat aaataccagg
    81121 acctttcagc attcctgcca aagtagcagc ctcagacagc gtaacattcg ttgcatctac
    81181 tccaaaatac tttctggaag cgtcttggac accccatacc ccgttgccaa aataggcatt
    81241 gttaagatac atggtcaaga tttcttcttt gctgtacttc ttttcgatct cgattgccaa
    81301 aaataattcc ttcgcttttc gatcaaacgt ttggtctaat gtcagatacg catttttggc
    81361 aagctgctga gtgatcgtac ttcctccgcc ccctgatgtt ccgccagaca agaccattcg
    81421 gactgctgca cgggcaatcc ctttgatatc aaacccgtga tgctcgtaaa aattcctatc
    81481 ttctgtcgag attactgcat cttggatata gggagaaatc gcgtttaatt ctacatatgt
    81541 tcctttttgg gcgtacagtg tgccagcttc ttcgtttttc ttatcataaa tgactgtcga
    81601 ttggcttaat cctgatttta acgtttcgac atttgcttgt tttgctaaaa taaagaggta
    81661 gatacttgtc actaagacac agatcaaacc aatcagcaaa atcaatttgt tcacatgata
    81721 ttttttccag atgcgctttc gccatcgatg gaattgtccg agatatggtc tgatccaacg
    81781 ccaaaaactg atcaaagcag cttttatctt ttctaaaatg ctttgaaaat ccatttgctt
    81841 cttctcctgt ctatttgatt caatcgtctt attttaccat agtgaaagag tgttttcttc
    81901 ctgcttaagt tgtagttcaa tatgtttttt tacgttagtg caagtagagt aacataagaa
    81961 tgaacagaaa aatgtaagtt ttttcttaaa tatgttttaa aaatggtaca attagtctgc
    82021 gattgcactg aaaggagtac acttgtgaaa atcacgatca ctttacctat gaaccatcct
    82081 gaaacaacaa ttcgcgactt gcttgaaaag gaatggttag ttcctagaaa ggttcgacac
    82141 tttttgcgta tacgtaaaaa cgtttggatc aatcaacaac ctgctttgtt ccatcatgaa
    82201 gtaaagggca atgaccagat cacattgtta ttcgaagaaa cagattataa ttatcaagaa
    82261 gttcaattag gagatgcgaa gcacatccgt gttttatatg aagatgagca tttgattata
    82321 gtggataagc ctgcaggaat gaagactcat ccaaatgaac cgacagaaaa cagtacctta
    82381 ctcaatcatt tagctgctta tctcgacact aaacagcaac gaccttacgt tgtccaccgc
    82441 ttggacaaag aaacaagcgg tgccgtttta tttgcaaaaa atccatttgt cttgccgata
    82501 cttggaagaa tgttggaaca aaaaatgatc tatcggagat atcaagccat cgtatgggga
    82561 gaaattgcca aagatatgac catcaccaat aaaattggac gagatcgcca tgatcggaga
    82621 aaaagggtcg tagatgataa aaatggtcag agcgccttga cacatgtcac tgtcaacaaa
    82681 gtactttctg aacaaacaca agtctattgt gttttggaaa ccggacgtac acaccagatc
    82741 agagttcatt tagctcataa gggacatccg atcgttggag accctctcta ccaaaataga
    82801 acagctgaac gactgatgct ccatgcatta gaactgcaca tgatccatcc atttactcaa
    82861 gaaaaaatag tcggtaaagc tttacctgga ttatgggaat agaaataaaa aagaacagag
    82921 tcttatcagc agataatgat aagactctgt tctttttgtg taatttgtct gcttactttt
    82981 gatgttcgtg ttctgctaac agcaaactac cgataatccc actttcattc ggcagtcccc
    83041 aacgaacgat atattcactt acttgaggtg tttccatata accacccatc aattcaacaa
    83101 attgctgacg gattttttga aggagatgat cctgattcat taccccaccg cctaaaataa
    83161 tcttttcagg tgctaacgtc aacgtatagt tgatcagtgc ttgagcaata taataagcct
    83221 gaatagccca aacaggatga tcttgcggca gatcttgccc tttgatccct gtgcgtgcct
    83281 caatagacgg tcctgctgct aatccttcta aacaatcttt atgataagga caagtacctt
    83341 cataatggtc atctggatgg cgtttcaccc aaatatgtcc catttcagga tgtgcgatcc
    83401 ctgaaaaaat ttcttcattg atcacgacac ctgcaccgat tcctgttccc acggtaaggt
    83461 aaatacaact attttttcct tgtgctgctc cttgcttcaa ttccccatat gcagcagcat
    83521 tgacatccgt tgtccaagca gcaggaatat tgtagcgttc cttgattgtg ccaagaaaat
    83581 cgaattgtgc ccaccctttt ttgggagttg ccaaaacgta tccgtaatta tcttttgttt
    83641 tgtcgatacc aatcgggccg aatgagccaa tcccgattcc cttcaattca taacgatcaa
    83701 aaaattcaaa tacctgatcc aatgtttctt ctggtgttgt ggtagggata cttaattttt
    83761 ctaaaatttc tccaccatct gaaaccgcac acacaaattt tgttccgcct gcttctattc
    83821 cgccatacat tcgttttcct cctcgaatcc gattgtccga atctcataag gttggattat
    83881 tgggacaacc gcttgcttcg ttggttcttc aagcagattc aatatgacag tttttccatt
    83941 gtctttagtt atttccaatt tttctaaatg actagacata ttgaaacctc ttactactac
    84001 tttatcacta aacttgctgc gtttcaaagc tgtaattgca aacgtttcac tttttatcgt
    84061 taaatattgt tgttttgaaa tcaatgttcc ttcatgatgt ttgatttgtt gagtggaaaa
    84121 aggaatctgt gctgcgtatg catgcaaata agtagagaat ttttcttctg gtccatgtag
    84181 ttcaatgctg taattgaacc gatgctcacc tagacactgt gcttctggtg ttggaaaata
    84241 tccccaatcg cctagttctc ccacactgcg aagtagcgtc actgcgattt gtccgtcttc
    84301 tgttacttca tattcattca aaccaaagtt tccgactgtt acaccgtact cttcatcatg
    84361 aatattgaca aatgcttgct gatgttgcgg attcgtgggg ttttcccaac ttttagacac
    84421 atggtttggg cgcttcacca cttcaaaaat actatctgct tcgtgatgtt ccacatgtaa
    84481 ctttgtagga aacaagacac gtaagcgatg atctttcatc tgattgtcga gagtcgtttc
    84541 aaagtcgatt tttttactat cttttctgat tgtcatgatt gtttttattt gaaggattcg
    84601 tttttctttg gatcgttctg cttttcgata acgaaattcc acaaccatct gctgttcttt
    84661 ttctaaacgc tcgtctgcag aaacagggat ttccatttcc tgtagaatac ttatttttgc
    84721 tatttccggt gtatctacta atacttttac ttcagagttt tctttgttac tggataggat
    84781 tggctgatca cagaaaggtt gtttaaagat atactcattt cctatatcgc cgatatcttc
    84841 aaaaatatgc agattattca actttcgttt gtttttgcga tcttccattg taatggtacc
    84901 atttttttca atcgtcaaat gtagagggcc gttttcgatg attcgtccac tctgatgaat
    84961 cattgtttcc ttttcttctg ctttcgcttc cccttctaca agcgcgaatg tttcccaaga
    85021 aaatgctggc atgttttcta atggtaaagt cactttgatc attcttgcca tatatggcac
    85081 acggaaacgg tctttcggca aatcataacc aaattggaca gtttcttcag ataaaatagc
    85141 tggtatagtg gccccacttt ttgtttctac atgatactta cgtttaggca aattttctaa
    85201 ctcttgatat agttgttcag gtattccttc agcgaatctt tttctttcta attcgatggt
    85261 tatttctgct tcaccagttt tatccattcc tgcagtattt acaatgacaa atggaaagct
    85321 gtcttttggg aatccggtcg tatcgattgc tgcggtcagc tgttctaagg aatcttgtgc
    85381 aagatacttt ccaacttcat ctgctttttc aaaacgaggt atcatttccc gatggacaga
    85441 atcgactgaa caaccgcaaa tactgtcgtg aggatgattt tgcattaatg tcttccaagc
    85501 ataatctaat tgatcatggg gatagtttcc actaacttca taagccatcg tcgcaagtgg
    85561 ttctgtaata ttttctagct gtcgctggac cttggtattc cattgtttta agtaaacccg
    85621 agcagatgct gtattggcta aagtatacca gccatccgtt tcttgacttg tcaattctcc
    85681 ttctactgag cctaagtctt ctggcacatc tttttgaact gcttccagat agtcagtaaa
    85741 attagaatga ataaactcgt aatctggaaa cagttcattt gcgagatgga ttgcttttga
    85801 tatatctttt tgtaccggtt gatgatcaac accattcatc atcaataagt ggttcgtaga
    85861 ggcatattgt tctgcatccg ctaatttttg cttccaaaag gcaagagctg cttctttttc
    85921 tactggaatc tcattcccgt tgctatacca attggcaaat agcagaccaa agattgctgt
    85981 ttgatcaggt cctttccacc acatctctga gtattgggaa gaatagtttt ctgcttccaa
    86041 aacttgatta tcaaagccaa ttggttttac accacggcca aacgctgctg ctgaaatacc
    86101 agcttgtttc attaattgag gcgtctgtcc catatttcca aatgtatcgg gaaaatagcc
    86161 taacatgaca ggggttcccc atttacggct ttcttccatg ccaatcagca tatttctcac
    86221 gttcgattcc gagctgataa gaaaatcatc ttgcaagata taaaatggtc caattctcaa
    86281 ttttccttca ttgattgctt gttggattgc ctgtcttttt tctggcctta cttgaagata
    86341 gtcatctaaa ataatcgttt gcccatccaa atgaaaactg ttgaaggacg gatcaacttc
    86401 aaatagctct agtagatcgt ctatcaattc aatcagtcgc atgtgatgct gttcgtaagc
    86461 catgtaccat tcacgatccc agtggctatg agaaatgata tatactttct ttttcattta
    86521 cgatacctcc tacttttcta ctctaatatc aaagtaatcc atgactaatt cgcaaaacat
    86581 catatttgcc caagaaaacc attctctcgt ataattttca ggattgttca catcgaatcc
    86641 ttcgtgcata aggtgtgttc ctgcgtcagt tgctacaagt aaattcaata tccgctcttt
    86701 ttcagatttg tctgacgttg tcattccttc catagctaat gcaatcggcc aaacgtattt
    86761 ttctggcgta tgggaggatc caattccttt tgcgtacttt ccttcataga aatatggatt
    86821 ttctttactc aacaatgttc ttcttgtttg cagataatct tcctcttgtt cagaacaata
    86881 tcccaagtaa ggtgcggcga tcagattggg cacattactg tcatccatga ctgtggcgtt
    86941 tcccattcca tctacttcat atgcataaat cgtttgccct ttttgattaa ccgttcgtcc
    87001 gtgattctga atcccctctt ggatctcttt cttcaatttc tgtgtttgct gtacaatttc
    87061 tttatcatcg agtatcgttt gaaaaatttc ttccacataa cctaacacga ccaccgcaaa
    87121 catattggat ggtaccagat atccataacg gcaagtatca tcgctaggac ggaaaccaga
    87181 ccatgtcatt cctgtaaaag ctgtttttgc acctcgcccg tcattgatca gcgtatcttc
    87241 ttttcttgtc gtgtctcgta caaatgtata aggagagtgt tcatgatctt gctctgtttt
    87301 gaacacgtcc aagatttttt tgataccttc aataaaagat tgattgaatt gatcagtttt
    87361 tcctgtattt ttgtaaagaa gataggccaa ctgtattggg tagcataaag aatcaatttc
    87421 atattttctt tcccagatcc aatcgttcat ttttgtatga tctgtttggt ggccagcacc
    87481 gttagcttct tcgttgaagg cattcgcata tggatcgata ttgatataaa aaaattgctt
    87541 tttgactaac ccgctgatca tgtcagctaa ttcctcatct tcttttgcaa tcactaaata
    87601 aggacgaact tgtgcagtag agtctcttaa ccacatcgct ggaatgtctc ccgtcaataa
    87661 aaaggtggtt ccgtcatttt gctttttgac tgttgtcaac agagtattgg caaatgctgc
    87721 tgtaaaattc tttgcccagt ccgcatggat atccccacat ttttgtgtga tatcttccat
    87781 aaatttctgc accgattttg gtatatcttt ataagccact aataaaacct tctttctatt
    87841 tgatatatct ttaaagatta tacttcatac agccaattat gtctacgctt tcaaaaaata
    87901 tctttttatt tactttttca tccattatgg tattatttga atatgatata tcattaagag
    87961 gtgtttatat gaatcaacca ttgtaccaga caatatttaa tgatttgcaa acggctattt
    88021 taactggtga attacctgta gacagccaac taccaactga aaaagaactt tcacgtacct
    88081 ataaagtaag cagaatcact tccaaacgtg cattgactga attagagcaa ttaggactta
    88141 tttatcgcgt tcgcgggaaa ggcagtttcg ttaaatccgc ttctccggaa cagattcttg
    88201 cttctcctac aaaaacacga cggattctgt ttcttcttcc atatgtcgct gatttgtctg
    88261 ttggtgattt tagcaaggga ttgaaccctg tgatgcaaga taaacagatt gatgtcatga
    88321 tgacgacgct tgactttcta caaaataaaa cagcaaaaga gctaatcaat gaatttgacg
    88381 gattgattta ttatgctctt gatacagaac aacatcttga cttacttttt gagctttcat
    88441 tgaaagaatt ccctgtcgtc atattagata aaaaaatcta tgagttacct tttcctaccg
    88501 ttctttccga taatttccaa ggcggtgcgc ttgctgcctc ttatttgatc gaacagggac
    88561 attcaaaaat cgcttatttg tttggtacgc agactcatcc acaatccgtt cgacagcgtt
    88621 accttggtta ccttgaagca ttggacaaag ctggtttgac tttccacact tcactggatg
    88681 acaaaagcgc gacattagat gaattgcttt cttatatcac taagtatgaa gtaactgctt
    88741 ttgtttgtga aaacgatgtt gtcgctatcc agaccatgaa tctcttgaga cagcatggtt
    88801 atcatatccc tgaagatttt tcaattatcg gatttgatga tatccaagca gcagctcttg
    88861 ttgacccgcc actgacaacc atcgcacaag attttgaaca gttaggtaag cttgcgggag
    88921 atgctttggt cacttggtta gagacaaaag atcgaccatc cgatagtaaa tttccagttt
    88981 cattcatcaa aagacaatca acaaaggaga aataaaatga atatcggtca aatagatact
    89041 agacatgcaa cagcgaatca gccaaatttc tctaatggga actgccagcc ttatactggc
    89101 gttccttttg ggatgaatta ttttgcaccg caaactacgg atcaaaacgg cagctggtgg
    89161 tttcatcctg atgaccgcgt ttttcaaggc taccgtctta cccatcaacc aagtccttgg
    89221 atgggtgatt tcagtcatat gctgtttacg ccgatcaatg gaaaactaca ggagaacacc
    89281 cttttccatg ctcaaagttc ttaccgccca gaagaaagta tattttgccc gactcatctg
    89341 tcgatctgtc aattacgtta tggcatctgt tctacattga tacctagcat gtatggcgga
    89401 atcttatcta ttgattacag caaaaatgac agcggtttac ttttatcctt tccgggacgc
    89461 catcagctct ttgttataga tccttacaca gtggaaggaa aaatcacaca ttttgctggt
    89521 tcagaagatc ctgatttttc tttttatttt atattgagat ttgaatatcc tttaacaatt
    89581 gatgagttag ctgtttccaa caaaaatagc gactcgatcc cgttgtattt tgaacagaca
    89641 aaaaaacaaa ctatccgatt tgggacatct tttatcagct cggaacaagc agcttataac
    89701 ctggaactag aacaaaacca aagcaaagag acttaccttg ctcacagtca agaacaatgg
    89761 cagtcctatt ttgatcgtat acagctcaca caccatgatc aacaacaatt gcggacgttt
    89821 tatcataatt tatatcgggt gtttttattt ccacaaacat tttatgaaat tgatcctagt
    89881 gggcaaaaaa tccactatga tacgactagt cgatctataa aacctggtgt tctctataca
    89941 aataatgggt tttgggatac gtataaaacg gtttatcctc tttattcttt gattgctgta
    90001 gaaaaatacg aagaaatgct ggaaggtttt ttgaacagct atagagaaag tggctatttg
    90061 ccaaaatggc tgtctcctga tgaacgtgga cttatgcctg ggacattgat cgatgcggtc
    90121 attgcagatg ctgctgtaaa agatatccga cgagacttga tgcctgaatt tctaacggca
    90181 atggaaaaag gtgctacggt tcaaagcaaa catccaaatt atggacgcca aggaacagaa
    90241 gattacttga catatggcta tgttcctaat cactaccacg aatcagtcaa tcacacatta
    90301 gactatgctt atagcgattt ttgtattagc caagttgcca aggtactaga agaagaggaa
    90361 atgacgaaga aatatcagca gcaatcattg aactatcaaa atatctttga ctatgagtca
    90421 ggatttatgc gttcaaaaga taaagatggc aagttcagag aaccattttc agctaatcgt
    90481 tggggacaag attatgcaga aggaagtgct tggcaaagca gctttgctgt ttatcacgat
    90541 ttttcaggac tgatcggaaa atatggtggc agtgagagat ataaagaaaa actcattgag
    90601 ctgtgcaatc agcccccaac gtttgaccca ggaggatatg gttttgagat ccacgaaatg
    90661 agtgagatgg ccgcgataga atttggacaa ttagctattt caaaccagcc aagttttcac
    90721 tatccttact tgttcagtta tataggacaa cctgaaatgg cacagccact gcttaaacag
    90781 ttgatgactc agaccttcga cgattctcca atgggttacc ctggagatga ggacaatgga
    90841 agtatggccg cttggtatat cttcaatagt ctgggatttt atcctgtcac gcctggaacc
    90901 ggtgaatatg ttatcggtat gccattaatt gacaaggctg ttttgctttt gtcaaatgga
    90961 aaggaattga cgattgaagc aacgcctaat cggccgcagc aacaatttat ccataaaatc
    91021 aaacggatgg acaaagaata tacgaaatta tattttaccc atcaagattt gctgaaaggc
    91081 ggcacgatct cttaccaatt agggatcatc cctcacccta agaaatatca agcaaaagat
    91141 ttgccttttt ccttatcaaa ctataattaa caagaggttg tgaaaaagct gtcctgcatc
    91201 aagtaataag aacagccaag caaaaataga tcctcatatt tttcgtttgg ttggtcttat
    91261 taccgcagat gcaagctttt gaacaccgtt tattgagagg ttgtgaaatc a
//
LOCUS       DACGLH010000004        83426 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.004, whole genome shotgun sequence.
ACCESSION   DACGLH010000004 DACGLH010000000
VERSION     DACGLH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.004"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..420
                     /locus_tag="IXT21_000254"
     CDS             <1..420
                     /locus_tag="IXT21_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ4867690.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="IXT21_000255"
     CDS             complement(504..1226)
                     /locus_tag="IXT21_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ4867691.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="IXT21_000256"
     CDS             complement(1216..1953)
                     /locus_tag="IXT21_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867692.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="IXT21_000257"
     CDS             complement(1965..2957)
                     /locus_tag="IXT21_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4867693.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="IXT21_000258"
     CDS             complement(2972..3715)
                     /locus_tag="IXT21_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4867694.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="IXT21_000259"
     CDS             complement(3956..5278)
                     /locus_tag="IXT21_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4867695.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="IXT21_000260"
     CDS             complement(5265..6230)
                     /locus_tag="IXT21_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867696.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="IXT21_000261"
     CDS             complement(6227..7765)
                     /locus_tag="IXT21_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ4867697.1"
                     /translation="MNSFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="IXT21_000262"
     CDS             complement(8181..8468)
                     /locus_tag="IXT21_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867698.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="IXT21_000263"
     CDS             8602..9270
                     /locus_tag="IXT21_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4867699.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="IXT21_000264"
     CDS             complement(9561..10301)
                     /locus_tag="IXT21_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867700.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="IXT21_000265"
     CDS             complement(10431..11378)
                     /locus_tag="IXT21_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4867701.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="IXT21_000266"
     CDS             complement(11507..12271)
                     /locus_tag="IXT21_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4867702.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="IXT21_000267"
     CDS             complement(12275..12664)
                     /locus_tag="IXT21_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867703.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="IXT21_000268"
     CDS             12799..13341
                     /locus_tag="IXT21_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ4867704.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="IXT21_000269"
     CDS             13489..14121
                     /locus_tag="IXT21_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867705.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="IXT21_000270"
     CDS             complement(14255..14380)
                     /locus_tag="IXT21_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4867706.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="IXT21_000271"
     CDS             complement(14514..15407)
                     /locus_tag="IXT21_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ4867707.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="IXT21_000272"
     CDS             complement(15589..16515)
                     /locus_tag="IXT21_000272"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ4867708.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="IXT21_000273"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="IXT21_000273"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ4867709.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="IXT21_000274"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="IXT21_000274"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ4867710.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="IXT21_000275"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="IXT21_000275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ4867711.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="IXT21_000276"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="IXT21_000276"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ4867712.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="IXT21_000277"
     CDS             complement(24786..25220)
                     /locus_tag="IXT21_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ4867713.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="IXT21_000278"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="IXT21_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ4867714.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="IXT21_000279"
     CDS             complement(26117..26992)
                     /locus_tag="IXT21_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ4867715.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="IXT21_000280"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="IXT21_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ4867716.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="IXT21_000281"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="IXT21_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ4867717.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="IXT21_000282"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="IXT21_000282"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ4867718.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="IXT21_000283"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="IXT21_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ4867719.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="IXT21_000284"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="IXT21_000284"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ4867720.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="IXT21_000285"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="IXT21_000285"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ4867721.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="IXT21_000286"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="IXT21_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ4867722.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="IXT21_000287"
     CDS             complement(35443..36210)
                     /locus_tag="IXT21_000287"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ4867723.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="IXT21_000288"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="IXT21_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ4867724.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="IXT21_000289"
     CDS             37323..37721
                     /locus_tag="IXT21_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4867725.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37958..38500
                     /locus_tag="IXT21_000290"
     CDS             37958..38500
                     /locus_tag="IXT21_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ4867726.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="IXT21_000291"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="IXT21_000291"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4867727.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="IXT21_000292"
     CDS             complement(39286..39513)
                     /locus_tag="IXT21_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ4867728.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="IXT21_000293"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="IXT21_000293"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4867729.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="IXT21_000294"
     CDS             complement(40048..40761)
                     /locus_tag="IXT21_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ4867730.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="IXT21_000295"
     CDS             complement(40764..41660)
                     /locus_tag="IXT21_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4867731.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="IXT21_000296"
     CDS             complement(41818..42660)
                     /locus_tag="IXT21_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4867732.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="IXT21_000297"
     CDS             42792..43445
                     /locus_tag="IXT21_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ4867733.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="IXT21_000298"
     CDS             complement(43508..44035)
                     /locus_tag="IXT21_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4867734.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="IXT21_000299"
     CDS             complement(44055..45002)
                     /locus_tag="IXT21_000299"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ4867735.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46900)
                     /locus_tag="IXT21_000300"
     CDS             complement(45014..46900)
                     /locus_tag="IXT21_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4867736.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47060..49138)
                     /locus_tag="IXT21_000301"
     CDS             complement(47060..49138)
                     /locus_tag="IXT21_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ4867737.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="IXT21_000302"
     CDS             complement(49151..49426)
                     /locus_tag="IXT21_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867738.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="IXT21_000303"
     CDS             complement(49788..50243)
                     /locus_tag="IXT21_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867739.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="IXT21_000304"
     CDS             complement(50397..51605)
                     /locus_tag="IXT21_000304"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ4867740.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="IXT21_000305"
     CDS             complement(51717..52049)
                     /locus_tag="IXT21_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ4867741.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="IXT21_000306"
     CDS             52197..53072
                     /locus_tag="IXT21_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4867742.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="IXT21_000307"
     CDS             complement(53154..53735)
                     /locus_tag="IXT21_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ4867743.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="IXT21_000308"
     CDS             complement(53741..55000)
                     /locus_tag="IXT21_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ4867744.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="IXT21_000309"
     CDS             complement(55002..56048)
                     /locus_tag="IXT21_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867745.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="IXT21_000310"
     CDS             complement(56283..56558)
                     /locus_tag="IXT21_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ4867746.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /locus_tag="IXT21_000311"
     CDS             complement(56801..58111)
                     /locus_tag="IXT21_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ4867747.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="IXT21_000312"
     CDS             complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="IXT21_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ4867748.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59657..60337)
                     /locus_tag="IXT21_000313"
     CDS             complement(59657..60337)
                     /locus_tag="IXT21_000313"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ4867749.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="IXT21_000314"
     CDS             complement(60386..61021)
                     /locus_tag="IXT21_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4867750.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="IXT21_000315"
     CDS             complement(61087..62490)
                     /locus_tag="IXT21_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ4867751.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="IXT21_000316"
     CDS             complement(62477..63508)
                     /locus_tag="IXT21_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867752.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="IXT21_000317"
     CDS             63539..63760
                     /locus_tag="IXT21_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ4867753.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="IXT21_000318"
     CDS             complement(63925..64662)
                     /locus_tag="IXT21_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ4867754.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64815..66155)
                     /locus_tag="IXT21_000319"
     CDS             complement(64815..66155)
                     /locus_tag="IXT21_000319"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ4867755.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66392..67915)
                     /locus_tag="IXT21_000320"
     CDS             complement(66392..67915)
                     /locus_tag="IXT21_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ4867756.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68293..68904)
                     /locus_tag="IXT21_000321"
     CDS             complement(68293..68904)
                     /locus_tag="IXT21_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4867757.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69027..69152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69234..69950)
                     /locus_tag="IXT21_000322"
     CDS             complement(69234..69950)
                     /locus_tag="IXT21_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ4867758.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="IXT21_000323"
     CDS             complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="IXT21_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ4867759.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70509..71306)
                     /locus_tag="IXT21_000324"
     CDS             complement(70509..71306)
                     /locus_tag="IXT21_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ4867760.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71276..71647)
                     /locus_tag="IXT21_000325"
     CDS             complement(71276..71647)
                     /locus_tag="IXT21_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ4867761.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="IXT21_000326"
     CDS             complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="IXT21_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ4867762.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72649..73122)
                     /locus_tag="IXT21_000327"
     CDS             complement(72649..73122)
                     /locus_tag="IXT21_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4867763.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73227..74090)
                     /locus_tag="IXT21_000328"
     CDS             complement(73227..74090)
                     /locus_tag="IXT21_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4867764.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74286..76385)
                     /locus_tag="IXT21_000329"
     CDS             complement(74286..76385)
                     /locus_tag="IXT21_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ4867765.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="IXT21_000330"
     CDS             complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="IXT21_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ4867766.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(77738..79579)
                     /locus_tag="IXT21_000331"
     CDS             complement(77738..79579)
                     /locus_tag="IXT21_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ4867767.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(79761..82154)
                     /locus_tag="IXT21_000332"
     CDS             complement(79761..82154)
                     /locus_tag="IXT21_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4867768.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(82261..83121)
                     /locus_tag="IXT21_000333"
     CDS             complement(82261..83121)
                     /locus_tag="IXT21_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ4867769.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(83180..>83426)
                     /locus_tag="IXT21_000334"
     CDS             complement(83180..>83426)
                     /locus_tag="IXT21_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HAQ4867770.1"
                     /translation="DYIAYVKNHGGENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQ
                     SLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
BASE COUNT        22930 a        17666 c        14209 g        28621 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtcggctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaacta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaagccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    58261 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    58321 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    58381 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    58441 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    58501 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    58561 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    58621 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    58681 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    58741 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    58801 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    58861 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    58921 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    58981 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    59041 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    59101 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    59161 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    59221 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    59281 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    59341 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    59401 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    59461 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    59521 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    59581 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    59641 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    59701 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    59761 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    59821 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    59881 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    59941 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    60001 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    60061 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    60121 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    60181 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    60241 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    60301 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttt cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    64741 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    64801 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    64861 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    64921 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    64981 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    65041 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    65101 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    65161 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    65221 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    65281 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    65341 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    65401 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    65461 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    65521 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    65581 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    65641 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    65701 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    65761 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    65821 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    65881 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    65941 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    66001 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    66061 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    66121 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    66181 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    66241 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    66301 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    66361 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    66421 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    66481 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    66541 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    66601 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    66661 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    66721 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    66781 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    66841 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    66901 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    66961 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    67021 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    67081 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    67141 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    67201 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    67261 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    67321 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    67381 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    67441 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    67501 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    67561 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    67621 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    67681 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    67741 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    67801 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    67861 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    67921 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    67981 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    68041 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    68101 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    68161 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    68221 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    68281 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    68341 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    68401 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    68461 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    68521 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    68581 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    68641 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    68701 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    68761 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    68821 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    68881 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    68941 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    69001 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    69061 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    69121 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    69181 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    69241 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    69301 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    69361 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    69421 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    69481 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    69541 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    69601 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    69661 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    69721 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    69781 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    69841 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    69901 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    69961 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    70021 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    70081 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    70141 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    70201 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    70261 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    70321 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    70381 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    70441 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    70501 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    70561 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    70621 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    70681 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    70741 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    70801 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    70861 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    70921 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    70981 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg
    71041 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    71101 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    71161 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    71221 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    71281 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    71341 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    71401 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    71461 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    71521 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    71581 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    71641 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    71701 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    71761 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    71821 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    71881 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    71941 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    72001 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    72061 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    72121 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    72181 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    72241 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    72301 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    72361 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    72421 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    72481 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    72541 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    72601 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    72661 gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc
    72721 ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc
    72781 ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc
    72841 gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat
    72901 tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact
    72961 cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa
    73021 aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg
    73081 ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat
    73141 ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct
    73201 agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg
    73261 ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct
    73321 ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt
    73381 tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct
    73441 tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact
    73501 cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac
    73561 ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt
    73621 cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt
    73681 ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc
    73741 aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg
    73801 acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca
    73861 gtaacgattc caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga
    73921 ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca
    73981 ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca
    74041 ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc
    74101 ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac
    74161 ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca
    74221 tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc
    74281 taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg
    74341 taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca
    74401 tatagattat aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct
    74461 tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc
    74521 cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc
    74581 tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt
    74641 gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct
    74701 tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc
    74761 tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga
    74821 tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct
    74881 gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat
    74941 tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg
    75001 ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga
    75061 tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc
    75121 ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga
    75181 ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat
    75241 tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc
    75301 tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc
    75361 cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat
    75421 tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt
    75481 tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc
    75541 tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca
    75601 gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt
    75661 atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca
    75721 gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct
    75781 ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct
    75841 actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct
    75901 tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca
    75961 ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct
    76021 tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt
    76081 ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt
    76141 tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga
    76201 tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca
    76261 tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga
    76321 ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt
    76381 ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt
    76441 ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc
    76501 ttcctataga aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa
    76561 accaaacagt tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct
    76621 tttaattgtt ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga
    76681 cgaatacgtt cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg
    76741 ccatcatcta aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct
    76801 agtacatctt ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt
    76861 gcttcctggt cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt
    76921 tcaagggaaa caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga
    76981 tccatttccg caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga
    77041 cgttggatac gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta
    77101 cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt
    77161 gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt
    77221 ccttcttgga tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcgatactt
    77281 actactaaac gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg
    77341 attttcaatg ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct
    77401 ttcagataca tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct
    77461 gtttttgctt gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca
    77521 ttttcatcca ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca
    77581 tctgcattca aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt
    77641 cgtttgggtt tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct
    77701 gttccatttg ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt
    77761 tcgttaaatt gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc
    77821 tggcttcttg ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga
    77881 tcacatctag caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt
    77941 cgatttccac caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa
    78001 ggttgaattc ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg
    78061 catcatgaat aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa
    78121 atgcacgata taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc
    78181 gtgcagtttc ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt
    78241 gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa
    78301 actctgttga cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa
    78361 gttcaactag taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc
    78421 cttgttttaa ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt
    78481 cttggaaaga tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa
    78541 cgactacgac agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg
    78601 ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt
    78661 ccatttgttg gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga
    78721 taccagcctg ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt
    78781 ttttccggat atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca
    78841 gttctgtttc tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg
    78901 caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca
    78961 ctctttgata aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc
    79021 gttctaacaa ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct
    79081 cattaggtgc aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt
    79141 gcaaatattc taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt
    79201 ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc agattcagga ttttggacag
    79261 ctatagtacg ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta
    79321 ccactgcttc tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc
    79381 ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg
    79441 tacgttcttc atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga
    79501 catattgtcc aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt
    79561 gaggaatccg ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca
    79621 gaagatattt caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt
    79681 aaaggacaca attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca
    79741 catttctatc tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca
    79801 gccttccagt acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct
    79861 acgctctctc ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca
    79921 tatatcccta cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa
    79981 gcttttacaa ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt
    80041 tcagcatttc cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact
    80101 acaagcaagg catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt
    80161 tttcttttat cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca
    80221 atcagcgtga tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc
    80281 gataaaataa atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg
    80341 ttgaccactc ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac
    80401 aataccagat tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa
    80461 tctgcttctc ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg
    80521 ccgctttctt tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa
    80581 accgtatagc gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg
    80641 acatacgcat cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga
    80701 ttgtctccag aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct
    80761 tgagcgtttt ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct
    80821 ggtttttctt cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca
    80881 ttcgcttggg ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc
    80941 gctcctacgt acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa
    81001 gaaaatgcga ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata
    81061 gcccgcatag tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg
    81121 atggctttct cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca
    81181 gtaatggtcc ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa
    81241 tacatgtctt gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca
    81301 atacttatta gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca
    81361 ataacagaaa ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc
    81421 cccaaaggaa taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt
    81481 tcagaacgaa tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct
    81541 gcacctactc ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc
    81601 aattcagctc cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt
    81661 gcttctgccc gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt
    81721 tttaccaaat ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg
    81781 atcactagta cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt
    81841 atttcttgat aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg
    81901 gaatacgcac ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac
    81961 gtcaatatat tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca
    82021 ttttgctggc cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc
    82081 tttttctcca ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc
    82141 aagattaaaa acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa
    82201 aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct
    82261 ttacttatat tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa
    82321 aagaacagga caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat
    82381 cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt
    82441 tgagaaaatg taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc
    82501 gatcggcaca atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa
    82561 tcccatatac accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat
    82621 caatccgccg gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg
    82681 agcgccgtat aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc
    82741 agaagtatct cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat
    82801 acatccgaca aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa
    82861 aaaagaaagt agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac
    82921 attttctttg aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc
    82981 aaagacagct gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa
    83041 cattcccttc acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc
    83101 cattttttgc cttttttcca ctatttgcgc actccttctt tcatttaatt ttattcatga
    83161 ttgaacattt cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat
    83221 tcattttgac aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat
    83281 tgtaaagcaa ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa
    83341 gtgggctcag cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat
    83401 gattttttac ataggcaata taatct
//
LOCUS       DACGLH010000005        80225 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.006, whole genome shotgun sequence.
ACCESSION   DACGLH010000005 DACGLH010000000
VERSION     DACGLH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.006"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            171..443
                     /locus_tag="IXT21_000335"
     CDS             171..443
                     /locus_tag="IXT21_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867771.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            458..1381
                     /locus_tag="IXT21_000336"
     CDS             458..1381
                     /locus_tag="IXT21_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4867772.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1521..2273)
                     /locus_tag="IXT21_000337"
     CDS             complement(1521..2273)
                     /locus_tag="IXT21_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4867773.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            2496..2858
                     /locus_tag="IXT21_000338"
     CDS             2496..2858
                     /locus_tag="IXT21_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ4867774.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3495..4967
                     /locus_tag="IXT21_000339"
     CDS             3495..4967
                     /locus_tag="IXT21_000339"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ4867775.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            4979..5803
                     /gene="nadE"
                     /locus_tag="IXT21_000340"
     CDS             4979..5803
                     /gene="nadE"
                     /locus_tag="IXT21_000340"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ4867776.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(5876..6766)
                     /locus_tag="IXT21_000341"
     CDS             complement(5876..6766)
                     /locus_tag="IXT21_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ4867777.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            6964..8481
                     /locus_tag="IXT21_000342"
     CDS             6964..8481
                     /locus_tag="IXT21_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ4867778.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8471..9742
                     /locus_tag="IXT21_000343"
     CDS             8471..9742
                     /locus_tag="IXT21_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4867779.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            9917..10813
                     /locus_tag="IXT21_000344"
     CDS             9917..10813
                     /locus_tag="IXT21_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4867780.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            10827..11690
                     /locus_tag="IXT21_000345"
     CDS             10827..11690
                     /locus_tag="IXT21_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4867781.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            11696..12259
                     /locus_tag="IXT21_000346"
     CDS             11696..12259
                     /locus_tag="IXT21_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ4867782.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            12269..13792
                     /locus_tag="IXT21_000347"
     CDS             12269..13792
                     /locus_tag="IXT21_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ4867783.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            13804..14763
                     /locus_tag="IXT21_000348"
     CDS             13804..14763
                     /locus_tag="IXT21_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ4867784.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            14849..15928
                     /locus_tag="IXT21_000349"
     CDS             14849..15928
                     /locus_tag="IXT21_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4867785.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(16180..17580)
                     /locus_tag="IXT21_000350"
     CDS             complement(16180..17580)
                     /locus_tag="IXT21_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ4867786.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            17798..19459
                     /locus_tag="IXT21_000351"
     CDS             17798..19459
                     /locus_tag="IXT21_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ4867787.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            19416..20114
                     /locus_tag="IXT21_000352"
     CDS             19416..20114
                     /locus_tag="IXT21_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ4867788.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            20130..21554
                     /gene="araA"
                     /locus_tag="IXT21_000353"
     CDS             20130..21554
                     /gene="araA"
                     /locus_tag="IXT21_000353"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ4867789.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            21861..23021
                     /locus_tag="IXT21_000354"
     CDS             21861..23021
                     /locus_tag="IXT21_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ4867790.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            23139..24998
                     /locus_tag="IXT21_000355"
     CDS             23139..24998
                     /locus_tag="IXT21_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ4867791.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25108..25542
                     /locus_tag="IXT21_000356"
     CDS             25108..25542
                     /locus_tag="IXT21_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867792.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(25981..27003)
                     /locus_tag="IXT21_000357"
     CDS             complement(25981..27003)
                     /locus_tag="IXT21_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ4867793.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            27131..27484
                     /locus_tag="IXT21_000358"
     CDS             27131..27484
                     /locus_tag="IXT21_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ4867794.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            27496..28662
                     /locus_tag="IXT21_000359"
     CDS             27496..28662
                     /locus_tag="IXT21_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4867795.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(28754..29374)
                     /locus_tag="IXT21_000360"
     CDS             complement(28754..29374)
                     /locus_tag="IXT21_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ4867796.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(29566..29862)
                     /locus_tag="IXT21_000361"
     CDS             complement(29566..29862)
                     /locus_tag="IXT21_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ4867797.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            30066..30716
                     /locus_tag="IXT21_000362"
     CDS             30066..30716
                     /locus_tag="IXT21_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4867798.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            30732..32459
                     /locus_tag="IXT21_000363"
     CDS             30732..32459
                     /locus_tag="IXT21_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867799.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            32456..34348
                     /locus_tag="IXT21_000364"
     CDS             32456..34348
                     /locus_tag="IXT21_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867800.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            35052..35921
                     /locus_tag="IXT21_000365"
     CDS             35052..35921
                     /locus_tag="IXT21_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ4867801.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            36130..37155
                     /locus_tag="IXT21_000366"
     CDS             36130..37155
                     /locus_tag="IXT21_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ4867802.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            37194..38144
                     /gene="manA"
                     /locus_tag="IXT21_000367"
     CDS             37194..38144
                     /gene="manA"
                     /locus_tag="IXT21_000367"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ4867803.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            38238..39941
                     /locus_tag="IXT21_000368"
     CDS             38238..39941
                     /locus_tag="IXT21_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4867804.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            39943..41976
                     /locus_tag="IXT21_000369"
     CDS             39943..41976
                     /locus_tag="IXT21_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4867805.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            41987..42427
                     /locus_tag="IXT21_000370"
     CDS             41987..42427
                     /locus_tag="IXT21_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4867806.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(42627..43754)
                     /locus_tag="IXT21_000371"
     CDS             complement(42627..43754)
                     /locus_tag="IXT21_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4867807.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(43757..44434)
                     /locus_tag="IXT21_000372"
     CDS             complement(43757..44434)
                     /locus_tag="IXT21_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867808.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            44698..46464
                     /locus_tag="IXT21_000373"
     CDS             44698..46464
                     /locus_tag="IXT21_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ4867809.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            46479..46916
                     /locus_tag="IXT21_000374"
     CDS             46479..46916
                     /locus_tag="IXT21_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4867810.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            46929..48080
                     /locus_tag="IXT21_000375"
     CDS             46929..48080
                     /locus_tag="IXT21_000375"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ4867811.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(48142..48840)
                     /locus_tag="IXT21_000376"
     CDS             complement(48142..48840)
                     /locus_tag="IXT21_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ4867812.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(48833..50185)
                     /locus_tag="IXT21_000377"
     CDS             complement(48833..50185)
                     /locus_tag="IXT21_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ4867813.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHGYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(50408..51808)
                     /locus_tag="IXT21_000378"
     CDS             complement(50408..51808)
                     /locus_tag="IXT21_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ4867814.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            51969..52706
                     /locus_tag="IXT21_000379"
     CDS             51969..52706
                     /locus_tag="IXT21_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ4867815.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            52831..53310
                     /locus_tag="IXT21_000380"
     CDS             52831..53310
                     /locus_tag="IXT21_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ4867816.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            53307..54074
                     /locus_tag="IXT21_000381"
     CDS             53307..54074
                     /locus_tag="IXT21_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867817.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            54304..54873
                     /gene="lepB"
                     /locus_tag="IXT21_000382"
     CDS             54304..54873
                     /gene="lepB"
                     /locus_tag="IXT21_000382"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4867818.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(54977..55579)
                     /locus_tag="IXT21_000383"
     CDS             complement(54977..55579)
                     /locus_tag="IXT21_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4867819.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            55685..56154
                     /locus_tag="IXT21_000384"
                     /pseudo
     CDS             55685..56154
                     /locus_tag="IXT21_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            56260..56844
                     /locus_tag="IXT21_000385"
     CDS             56260..56844
                     /locus_tag="IXT21_000385"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ4867820.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            56847..57920
                     /gene="prfA"
                     /locus_tag="IXT21_000386"
     CDS             56847..57920
                     /gene="prfA"
                     /locus_tag="IXT21_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ4867821.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            57913..58752
                     /gene="prmC"
                     /locus_tag="IXT21_000387"
     CDS             57913..58752
                     /gene="prmC"
                     /locus_tag="IXT21_000387"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ4867822.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            58745..58924
                     /locus_tag="IXT21_000388"
     CDS             58745..58924
                     /locus_tag="IXT21_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867823.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            58946..59971
                     /locus_tag="IXT21_000389"
     CDS             58946..59971
                     /locus_tag="IXT21_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ4867824.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            60146..61390
                     /locus_tag="IXT21_000390"
     CDS             60146..61390
                     /locus_tag="IXT21_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ4867825.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            61543..62490
                     /locus_tag="IXT21_000391"
     CDS             61543..62490
                     /locus_tag="IXT21_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4867826.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            62624..63253
                     /gene="upp"
                     /locus_tag="IXT21_000392"
     CDS             62624..63253
                     /gene="upp"
                     /locus_tag="IXT21_000392"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ4867827.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(63392..63634)
                     /locus_tag="IXT21_000393"
     CDS             complement(63392..63634)
                     /locus_tag="IXT21_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867828.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            63755..63827
                     /locus_tag="IXT21_000394"
     tRNA            63755..63827
                     /locus_tag="IXT21_000394"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63788..63790,aa:Thr,seq:cgt)
     gene            complement(63946..64584)
                     /locus_tag="IXT21_000395"
     CDS             complement(63946..64584)
                     /locus_tag="IXT21_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ4867829.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(64707..65339)
                     /locus_tag="IXT21_000396"
     CDS             complement(64707..65339)
                     /locus_tag="IXT21_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ4867830.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            65690..65989
                     /locus_tag="IXT21_000397"
     CDS             65690..65989
                     /locus_tag="IXT21_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4867831.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            66484..68649
                     /gene="acm"
                     /locus_tag="IXT21_000398"
     CDS             66484..68649
                     /gene="acm"
                     /locus_tag="IXT21_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ4867832.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(68921..69406)
                     /locus_tag="IXT21_000399"
     CDS             complement(68921..69406)
                     /locus_tag="IXT21_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ4867833.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            69886..70527
                     /locus_tag="IXT21_000400"
     CDS             69886..70527
                     /locus_tag="IXT21_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4867834.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            70546..71181
                     /locus_tag="IXT21_000401"
     CDS             70546..71181
                     /locus_tag="IXT21_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867835.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            71199..72014
                     /locus_tag="IXT21_000402"
     CDS             71199..72014
                     /locus_tag="IXT21_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4867836.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            72333..73769
                     /locus_tag="IXT21_000403"
     CDS             72333..73769
                     /locus_tag="IXT21_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4867837.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            73958..76405
                     /locus_tag="IXT21_000404"
     CDS             73958..76405
                     /locus_tag="IXT21_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ4867838.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            76635..77702
                     /gene="rlmN"
                     /locus_tag="IXT21_000405"
     CDS             76635..77702
                     /gene="rlmN"
                     /locus_tag="IXT21_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ4867839.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(77895..78998)
                     /locus_tag="IXT21_000406"
     CDS             complement(77895..78998)
                     /locus_tag="IXT21_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4867840.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            79551..80099
                     /gene="aac(6')"
                     /locus_tag="IXT21_000407"
     CDS             79551..80099
                     /gene="aac(6')"
                     /locus_tag="IXT21_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ4867841.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        26820 a        13796 c        16508 g        23101 t
ORIGIN      
        1 ttcactaata ataaagatat atttacagta tcattataat gtattcaatt taaaataaga
       61 gactttggta ataatgaaag aggaataaaa cttttctttg gcaaaacaaa ctccttgcta
      121 tgagacgcgt ttcatacact atactcaaag tatcaaaagg agggcttgca atgaatactt
      181 atgtagagat aacagatggc acaacgaaca attattttta tgcgtttgtc gagttcaagg
      241 gatccgtatt aacgttgtac agttttcaag ggttgaatag gaatatagta aaagaaatac
      301 caatgaatga aatcgaaaat ttgacaaaag atatatattg gggcgggcag cggatcagtt
      361 tttcgcatga aggaaaaatg tatcagtttt ttgaatgcgg tccagctgtg gtagactatt
      421 tacaagaaaa tctttttgta taggtggcga agcagcagtg gcaaacattc gagatatcgc
      481 aaagctcaca ggttattcag tttctactat ttcacgtgta atcaatggac atccatatgt
      541 agatgaggaa aaaaggaaag aaatacttgc ggtcatgaaa gaagtcgatt atatccctaa
      601 tgcaaatgcc agacagttaa gttatggaaa aacgaaaagc attggagtga ttctgcctta
      661 taccaatcat ccgtattttg atcagttgat cagtgggatc attgaagcag cgtttgatta
      721 tgattacaaa gtgacacttt taccgacggg ctatcataaa gaaaaagagc gaaggtatct
      781 tgaagaattt gcagctaaag ctttcgatgg attgatcatt acgtctcgtg ccaatacact
      841 agatgattta ttggcatatc aaaaatatgg acctctagtt ttttgtgaag aaatcaaggg
      901 aaaacaggca agctgtgtct ttattgatcg agaacaatca attacagaag gtcttactta
      961 tttgaagcag caaggtataa agcaattggg catcacgttg gggcgaagtg gcaggctaag
     1021 ttataactca aaaatcactt tgcagctatg tcatcaactt tttccatctt ttgatgaatc
     1081 acttgttttt tgggattgta tagatgcaga gaaaggaaaa caagcaggct tcttttttaa
     1141 agagcttgga gttgatggtg tctttacaaa ttcggatatg gttgcagcag gcatcttgca
     1201 aagctatctg caagataaga caccagtggt gattggacgc gataatttgc tgatcagcga
     1261 gcttcttggt ttcccaacca tcgaccacca tctggaagct tgcggagaaa tggcatttca
     1321 attgtttctt acagaaaaaa aggataaagt gaaaatacct tatacgttta ttcaacgatg
     1381 aatatattta aagtaggatg aaaaaacttg tgaaagaatg gagcagacac caaaagttag
     1441 cataaaaatc taacttgggt gtctgctgtt ttgttattag gtcattttac gtgtactttt
     1501 ttgttggatc atgtaaaaga ttacgccttt ttttggataa tttcatttaa agcttttgct
     1561 gcaccatgac cacttcgctc gtcataatat gcagtgaacc gttcatcggc ttcgtacatc
     1621 attcctagtc ctttatgcgc ttttggctga tattcaggcc agctgtaaga gaaccacttc
     1681 ttatgagctt ggaagatacg gtctgctaag ctttcatctt ctggttcttt taagtaaaga
     1741 gtgagatcaa ataacatctg cttttcaaca gtttgcattt cttcatacgt ttctttatcc
     1801 atattcatcc atttttcatt ggatgctgta accttttttt ttccgtattt ttgtcgaatc
     1861 tcttgtccat aggattcttc attttctttc aacttttgtt gtttaaaccc ttcaaatttt
     1921 tctttgtcac tcatttttct tcctccttcg tacatatcaa gtgtctgttg gacagttgtc
     1981 aagagggtat cgatttgtgc ccgtttttcc agcaacattt gctgatgctt gattaaagct
     2041 ttctggtggt caaaagatgg attgtctaag acctcttgga tatcatccaa cttgaatgcc
     2101 attgttcgat aaaataagat gtgttgcaga cgatcgactt cttttttgcc ataaatccga
     2161 taacctgaag aattgatcct agctggtttt agcagtccaa tctcatcata aaagcgcaag
     2221 gttctcggac tgactcctga tatctctgac attttcttta ttgtgtactc cattattttt
     2281 cctcctgaca aatgtaagca taatccttta cgtaacgaga tggtcaagaa aaaattgtat
     2341 ttttttctca cgaaattatg ttatactatt aagtgataaa aggtaaatcc ttttgtcttt
     2401 aaaggttcaa ttgtaaccga ttccttaatg ctaggaaaga tggtaacata acttaagatt
     2461 ggatctaaat cgactatata gaaaagagga atgagatgaa gctaatcgaa acacctgtga
     2521 atagtaattt aaatattaag acattttatc ctaaagtagt ggaattcttt tttgggaata
     2581 cggctattaa ttattacaaa ttattttcgt tagatcgaac acaactgcta ttggtagaca
     2641 catatgataa aaagcaagtc gtgatgatca atacgaagaa aaaaatcaca aggcaagaaa
     2701 tcgactatgc gattcatcac gttttaaaaa tgacacgaga agatgtcaaa gtccatgtag
     2761 gagtgaaaca ggagctagaa cgtgccggaa tacaattcaa acgtccaaat aaagatattg
     2821 tagtgataga acaaaaaaat acgaaggatt ctatttaata acgactaata atcatcttta
     2881 tggtaacctg ctccatatct aatgtataca ataaaaaaca aggcgagact gattgatgtc
     2941 tcgccatttt tgtttccatt ctattgacag ctgctttctt actttgtatc tttttaaaaa
     3001 cgtgtgatat tttttccgcg agttttttca acgcaaggta tatcttgtca taattcgcct
     3061 ttccaaaaaa attcaataca taataagtta tatccgtttc tttggggata agaagcaaaa
     3121 aaggaaagac gtattattgc ttcaaaaact tttgccattg attatgttgt agagacattt
     3181 ggttatattt gttcaatcat cggtatctta acggctacga ttgtagtagg aaaatctact
     3241 tattttatca agtaagaaaa atagaaaacg ctaatttctt cgtgtctcgg atacattgaa
     3301 taaaataagt attctttctc gtttaggtta aataccaata agaaaggata gttttagcag
     3361 atggagttca tttttagcaa gaaatatccg taaaacatgt gctttttcag aaaaaatgga
     3421 agatttcttt tcctattaat tttttttgtt ataatgggta ggaaatgaaa acttgccaag
     3481 aggagtaata caagatgaat aatacttacc cagacgacag tttgacgctg catacggatc
     3541 tatatcaaat caatatgatg cagacatact gggaattagg tcgagctgat ttacatgctg
     3601 tttttgaatg ttactttcga gaaatgcctt ttaaccacgg atacgcggtt tttgcaggat
     3661 tggaacggtt agtcaattat ttagagaatc tgacgttcaa tgattccgat attgcttact
     3721 tgcgtggatt agaagtctat cctgaaggtt ttttggagta tttacaaaat tttgaattta
     3781 aagcaacagt tcgttctgct cgtgaaggag aactagtttt tgcaaatgaa ccattaatcc
     3841 aagtggaagg gcctttagcg cactgccaat tagtagaaac agcactgttg aatatggtca
     3901 atttccaaac gttgatcgcg acaaaagcag ctcgtatcaa atctgtcatc ggagaagatc
     3961 cactattgga atttggaact agacgggcac aagaactgga tgcagcagtc tggggaacta
     4021 gagcggcata tatcggcgga gcagatgcaa caagcaatgt acgtgctggg aagatatttg
     4081 ggataccagc tagcgggacc catgctcatt cgttagtcca atcttatgga aatgattatg
     4141 aagcatttat ggcctacgct aaaacccata aagactgcgt gttcttagtc gacacttatg
     4201 atacgttgaa atcaggtgtg ccaagtgcga ttcgcgtggc gaaagaatta ggtgacaaaa
     4261 tcaacttcca aggcgtacgc atcgacagtg gagatatggc ctatatatct aagcgtgttc
     4321 gggaacaatt agatgcagcc ggatttacgg aagcaaaaat ctatgcttcg aatgacttgg
     4381 atgaagcaac gattttgaat ttaaagatgc aaaaagctaa aattgatgta tggggtgttg
     4441 ggacaaaatt gatcacagcc tatgatcagc cagcccttgg tgcagtattc aagctagtat
     4501 cgatcgaaga tgatgaagga aaaatgatgg atacgatcaa gttatcaagc aatgcagaaa
     4561 aagtaactac tccagggaaa aaacaagtgt ggcgcatcac tcgaaatttt gatgggaaat
     4621 ctgaaggaga ctacgtgaca ttatgggatg aagaccctcg agaagaagaa gctatcttca
     4681 tgttccatcc agtgcatacg tttatcaata aaactgttcg agactttaca gctcgcccag
     4741 ttttacaaga tatctttatc gaaggaaaaa gagtttacga attacctgca ctaaacgaaa
     4801 tcaaagaata tacaaaagaa aatctagatt cactatggga agagtataaa cgagacttga
     4861 atccgcaaaa atacccagtc gatctgtcta ctgaatgctg gaaccataaa atggctatca
     4921 tggaacgaat gaaaaaaagc gtagcagaac ttcatacaga agcataggag gagaataaat
     4981 gagcctgcag acagaaatta tcaatgaatt aggtgtcaaa ccaacaattg atccaaaaga
     5041 agaaatacgt aaaagtattg actttatgaa agcatatttg aaaaaatatc cgtttcttaa
     5101 aacttttgta ctaggtatca gcggcggcca agattctact ttagcaggcc gtttggcgca
     5161 actaacgatg gaagaaatgc gtgaagaaac aaaagatgaa aactatcaat ttatcgctgt
     5221 tcgtcttcct tatggtgaac aagcagatga agaagatgca aaagctgctc ttgactttat
     5281 ccagcccgat gttagtctgc gagtaaatat caaaccagct gttgatgcac aagtacaagt
     5341 attaagcgaa gcaggtgtgg gaatcagcga ttttaacaaa ggaaatatca aagcacgtca
     5401 acgtatgatt acgcaatatg ccgtagctgg agaaagagca ggagccgtat tagggacaga
     5461 tcatgctgca gaaaacatta ctggattctt cacgaaattt ggtgatggtg gagccgacat
     5521 tttgccatta ttccgtttgg acaaacgaca agggaaacaa ttgcttcaag cattgaatgc
     5581 accggaaaaa ttatacacaa aaattccgac tgcagatctt gaagatggga aaccaatgat
     5641 tgcagatgaa gtcgcactag gtgtcacata taatgaaatt gatgattatc tagaaggaaa
     5701 agaagttccc gctcaagcac aagaaaaaat cgaagcatgg tggaacaaga cacaacataa
     5761 acgacatttg ccaatttccg ttttggatga tttctggaaa taacaataaa gcaaaaacag
     5821 ccgtcccttc atagggattg gctgtttttg ttttataaaa tagaaaagaa aaatatcagc
     5881 tgtagtaaac ttttaatttg atatcctgat cgtaattacc gaatccttta ccaaataaag
     5941 tacagccccc cacatgtttg gcatcgtctt tcacttcgat tcgcaaatgc caagtgtctt
     6001 gctctttagg gatgtcgttg atcgtgatgt ttgacaaggg ttctccattc atattcgtca
     6061 agtgtttggt aattcggatc gtcttcagca agccgtattg attaagatta tctggatacc
     6121 aatcaggcgt atatttaccg cggatatcag caaaatctcc agggctcgtc catgttccta
     6181 gctccacacc atttaggctg aaagtgatat ctgaaggcca attgtcattt gaaaaaggaa
     6241 attcagaaga aatttccaca gaaacttcca acatctccaa tgtatcctct gtatttaaaa
     6301 aattaggtgc ctgatactcg acaaatccgc tggtaaacca gagaatgcgg gcatccatcc
     6361 ttcgaggatc cataaaatag cgtggttcat ccactttccc gataaaatca tggatcgtag
     6421 ctaaaccgca tgttggttca attgcataat ttgtatagtg accaataggt atcgatgttt
     6481 ctttcgtatc aaaagcattg aatatttttt ctggaaaact gatatcgatt ttatcgacac
     6541 gaaggctcga aattttttgt tgtcctactt tttctgtttt gatgatgttt gcttcctcta
     6601 gctttttcac gtgcatcgtc gtgatagcac tgctcactcc aagttctttt gccaaatctt
     6661 tgacattcat cttttttttg gaaagtagct ggatgatctt gatccttgtt ttactagcta
     6721 atgcttcata taccggtaag gattcttcgt ttatttgtag ttgcatatat atcacctctt
     6781 aaaaggtttt tcttatatat taacatattt gttaataaaa gttatattaa attatttgtt
     6841 ttaatcatta ttaatatttt ggttaattaa aatcgataaa aaataagtaa gtttaacatt
     6901 tatattattg acaacgcttt caacgaggtg tattattcat gtataaatta aaggaggtca
     6961 gttatggaag ccacaatgtt gattagtagt gtcaagcgtg taggcaaagt tgatccgcgg
     7021 ttgtacagtt catttattga acatatggga cgagcagtat atgaagggat ttatcagcca
     7081 gatcatccat cagctaataa aagtggattt cgccaagacg tcattcactt ggtaaaagaa
     7141 ttgaacattc ccatgattag atatcctggt ggaaattttg tgtcaggatt tcgttgggaa
     7201 gacagtattg ggcccatagc aaatagacct agaaggttgg atttagcttg gcggacaatt
     7261 gaaacgaatg aagttgggat tcatgaattt tataactggt gtcagcatgt gggggccgaa
     7321 atcaatatgg ctgtcaatct gggtactcgt ggagtagatg cagctcgtaa tttagtcgaa
     7381 tattgtaatt ttcctaaagg aacttattgg agtgatttaa ggcgttcgaa tggtcaagag
     7441 agcccttttg gaattaagac atggtgttta gggaatgaaa tggacggtcc ttggcagatt
     7501 ggacataaaa cagcggaaga atatggacgg ttagcagagg aaacggctaa ggcgatgaaa
     7561 ctcgtagatg attcaattga ggtggtttta tgcggaagtt cgaatcggca aatgcctaca
     7621 tttggtgatt gggagttaac agttcttgat caagcgtacg atcaaataga ttatttgtct
     7681 ctgcatcaat attatggcaa ccaaaaaaat gatctttctc attatcttgc acgttcctta
     7741 gacatggatc ggttcatttc tgaagtgaca gcgatgtgcg atacagtcaa ggcgaagaaa
     7801 catagtaaga agcagatcaa cctgtccttt gatgaatgga atatttggta tcattcagcg
     7861 gaacaagatg agcaagcagc gccttggcag attgctcctc ctttactaga agataactat
     7921 aattttgaag atgcattact attaggctgt ctattaatca ctttattgaa acatgcagat
     7981 agagtaaaga ttgcttgtct tgctcaattg gtaaatgtta ttgcaccaat caggacagaa
     8041 acaaacggag aagcttggag acagacgatc tattatccat ttatgcaagc tgctgtgtat
     8101 ggacaaggag aggtgttaag cccacaaatt cgttcagcaa actatgcgac ggaagatttt
     8161 gaagaagtgc cgtatttaga aagtatagcc gtaataaatg acaaagaact tgtcatattt
     8221 gcagttaacc gtggagaaga agaaatgaat ctaactgttc acttgtctga attatcatta
     8281 gagggagtga ttgatttttc tgaaatgagt ggatttgata caaaagcaac taatcttcct
     8341 aaaagtgaac aagtaaaacc aagtcattca acaaatgtaa aagctgaaga tgggaaaatc
     8401 aattgtttgt tgaaaccgct atcttggaat gtggtcagat gtcgtttatc agatgaagga
     8461 ggaacaaaaa atgaaaggta aaaaatggtt aattggctct ttgttggtag ctgcaacagg
     8521 tattttagga gcttgcggtg gcggaagttc agcagaatct gattctgata aagaagtgac
     8581 tttgactttt tggaatgggt ttactgcatc tgatggtgaa atattgcaag aaatcgttaa
     8641 tgaatttaat aaaacaaatg ataaaaacat caaaattgaa atggatatca tgacttgggc
     8701 caatttgaat gaaaaacttc ctacagcgat ttcttctaaa aaagcacctg actttattgc
     8761 attaaattat ccagattttg cacagtatgt agaaaacgga gcggtacaac cattagacga
     8821 tttctgggaa tatgatggag tagataaatc tgattttaaa gaaacggcta ttgaattagg
     8881 tgaagtagaa ggtaaacagt attttgtccc catgcaggta caaggcatgt atatgtattg
     8941 gaataatgat ttgtttaaac aagctggatt agatactgaa caaccaccta ctacttggga
     9001 agaattgaca gaaatggcac caaaattaac tgatagtagt aaaaatgtat caggttttgt
     9061 cttcaataag gatggaactg caccgttata taactggatg ttagcaaatg gcggtaaatt
     9121 agtgaacgat gattatacta agtctgaatt tgcgtctcct gagaacctta aaacgttaaa
     9181 agcgatccaa aaaatgatcc atgtagacaa gactggtcca gaatcgattt ctggcgccga
     9241 aatggacaat ttaatgaatg ctggtcaact agccattgaa atcaatgggc catggttaaa
     9301 taacggattg aaagcaaacg agattgatta taatgtgact accttaccac aagggagtga
     9361 ttcagacaaa aaaacagcta ttttagatgg cgtaggatat gccattccgg caagtacaga
     9421 taataataaa aaagaagctg tctatgagtt tttgaaatat tggaatacaa ctgaaattgg
     9481 taaaaaatgg agtatcgaaa atggttttcc tgcctacttg gattcagtag caaaagatga
     9541 agaagtaaaa aatgatccta ttgtatctga attatcgaaa caaatggact atgcagagcc
     9601 atatttacca ggttttaccc aaatctctgc gatcaataac gatatcatca atccactgat
     9661 tgaaaaatta ttagcaggag aagatcctga gacactcatg aacgatgcag atgatgccat
     9721 caatcaatta ctgcaaaaat agtacagaac tttatacagg atagactgtt ggcaagatac
     9781 agatatctga atgatcgacc tagtatttgc agataggtgt tattgcaaaa atcacagcga
     9841 ttgttcggat atcttattca gattcgttta aaccaagatt gtatatttta tcaaaatgaa
     9901 agatgaggtg gactatatga acggattcac caaatattgg aatcgtcctg atcgtgctgc
     9961 gtatttgttt ttaactccat cttttttagt tttattatta tttactgtca taccgctttt
    10021 aggaacattt gctattagtt ttacagattt gaatattttc tttacatcaa gaaattttgt
    10081 aagttttgaa aatttcacac gtatatttag tgatgaacga gcagttaatt cgttgattca
    10141 ttctttatat ttcaccgtac tcgaaacgcc aacacaaatt atcgttggtt tattagcagc
    10201 agtagcatta tctaaaaata ccagattcaa tcgtttttgt cgttcgacat tttacattcc
    10261 agttatttgt tcattgacgt caattgcgat tattttttct atgttgcttg atccgaatgt
    10321 aggggcgatt ccttacttgt tttcacaagt cggcttgccg atccctcaat tttttcgcga
    10381 tccaaccctt gctatgccga cggtagccat catgactgtt tggaagaatt ttggtgttac
    10441 tatgacgatt ctcttgactg ctattcaggg aatctctccc tctttgtatg aatctgctca
    10501 aatggatggt gcaacaaatc gacagcagtt ttttaacatt acgctacctc agatcgttcc
    10561 ctccttaggt ttttgtattt taacgaatct gattggttcg atgcaagtgt ttgaccaagt
    10621 atatgttgca accgctggtg gcccacaatt taagacagaa acagctgttc aatatattta
    10681 cagtcgagga tttacagcac cttatgaatt gggctatgca tcagcactgt cttcggtttt
    10741 gtttatcatt gttgcaacta tagctatctc cactaatgtg tacatgtcac ggaaagaaaa
    10801 acaaatgaaa taggagggaa acctgtatgg caccagaaag ctcgattaca gttactgtga
    10861 atagtcgaaa agcagttagt ggtaaaaaaa tcattcgaac gattagtatg ctagttatat
    10921 tgttggttat tgcgtttcct tttttgtggc tgattatttc ttcttttaaa cacgaaaaag
    10981 acattatctc atttccgcct agaatatttg cagatagcta taccctagat aactatatta
    11041 aggtttggac aacgatccct ttattagatt acatcaaaaa tacagtgatt tttgctggag
    11101 ggacagtgat cacttctgta ttctttgatt ctcttgcggg gtatgcattt gctcgtatgc
    11161 gtttcaaagg aaaatcagtt ttgttctatt tcgttttgct gacaatgatg atcccattcc
    11221 aagtcttcat gattcctcta tttattgaag tcaatttgct tggcatgctt gatacttatg
    11281 caggtttgat cattcctcgt atgacaactg catttggtat cttcatgatg cgttcctttt
    11341 ttattacttt accagattct ttggaagagg cagcacgcat tgatggatta agtgagttta
    11401 atattttttt gaaaatcatg ttgccattga gcaaacctac tcttttatca ttagggattt
    11461 tcacgctgat gaatagttgg aatgacttgc tttatccatt gatattgaca agtagttcaa
    11521 aaatgagaac tttaccagca gggttagctt tatttacagg gcagaacatt tctttttatg
    11581 gacctgtaat ggctggaacg gttatttcaa tgctgccatt actagtggtt tatatttttg
    11641 cacagaaata ttttgttcaa ggaacagcta tgtctggaat gaaagagtga ggaagatgaa
    11701 aatagttgag actaaacttt atggacagtt gattgtggtt tctgattaca tcttattagg
    11761 tattctctgg gtaatcgtgt gtttgcctgt tattactatc gtgcctgcta catgtgctgt
    11821 tttttatgta atggcgcaat ggaaaggtga gggatcagga aatatcattt attttttctt
    11881 tcaaggaata aagcaacatt ttggtaagaa cttgttcctt agtctgttaa cgatcattat
    11941 ttattttctt gttaatcaat tattagaaga acagacaatc gtactttcaa ttagtggata
    12001 tgtagtttta cttatttatt ccatgtttct tttatcctgg atatatgtga tttctgaaaa
    12061 caaagatatc acatttttac aaatttttga acaaagtgca tggaaagtat tttttgattt
    12121 tttgagaaat attctttgtt gcgtattgtt attaggattt gtactgctca tatttctttt
    12181 ccctcctttt atttttatct ttgctggtgg cgtatggaaa cttgtatcta ttatcttgac
    12241 tataggaaat gaaaggtagg gaaagctgtt ggacgaagaa gtgaaagtaa aaatcagaga
    12301 ggattttacg gtaggagaga tcagttctcg gctgtttggc tcatttgttg agcatatggg
    12361 aagtgtcgtc tatacaggga tttatgaacc gggccatgaa acagctgatg gtgacgggtt
    12421 tagacaagat gtcctctctt atgtaaagga attagaatta ggtgtcattc gctatcctgg
    12481 aggaaacttt acatctggtt atgattgggt ggatactgta ggtaggaaag aacaacgacc
    12541 aaaaaaaatt gatttagcat ggcgaggtat agaaccaaat tcttttggac tccacgaatt
    12601 ttttaaatgg atcagtcatg tacaagcgaa accgatattc acggtgaatt taggaacaaa
    12661 aggaattgat gaagcaaaaa atttagtaga atattgtaat tttccaaaag gaaccttctg
    12721 gagtgattta agaataaaaa atgggcaaaa aaagcctttt ggcgttaaat tatggtgttt
    12781 agggaatgag ctagacggtc catggcaagt gggggctaag agtgcttatg aatatggtag
    12841 gttagcaaat gaagcctcaa aagtcatgaa gttagtggat gattcaattg aaacaatctt
    12901 agttggaagc tctacgccac gtttaccatc gtaccctgaa tgggatcgta tcgcattaga
    12961 agaagcttat gagaatgtag attatttagc tttgcataac tatattgata agtatgatga
    13021 agaagacttg actaaacctc ctaaaaatga gcgtgatgat acagcgacat accttgcaaa
    13081 agcttatcga ttcgatcgtc aaattgaaga aatcgttgct acttgtgatt atgtaaaagg
    13141 atatttgaga agtgagaaaa caatgtatct agcgtttgat gaatggaatg tacatgccca
    13201 accggaaaaa agacataggg attttgaagt cggtagtccc atcgattggt gttactttac
    13261 tatggaagat acattattat ttggttcatt agggctggct attttacgac acgcagatcg
    13321 cgtaaagatt agttgtcaat cattgttagt aaacacaata cctttaatac tgacagatgc
    13381 taaaggacaa gcatggagga atccaactta ttatgtgatg caacacttat cgaaatatgc
    13441 taaaggtcaa gtattgaaac aacaaatcat ttgccctgtt tatgattgtg aaaagataaa
    13501 aaatgttcct tatgtggaca gtgtgatcgt agataacggc aatcagctga ctattttctt
    13561 gatcaatcgc acaaatgaac aacaaaagat aacgattgaa catgatttca atttgctaga
    13621 tcaaggagag cactacacat taaccagtag cgatctgttg gacaaaaata cacgtgaaga
    13681 accagaaaaa ataaaacctg aaataaacta tttttcaata gaaaaacatg acgaattaaa
    13741 tgtagggata gaaaaatact cttggaatgt aattagaata aagaaaatgt gaggaaattt
    13801 gttatgatga agacacttta taataatcct atcgttcggc aacgggcaga tccttgggta
    13861 tataaacata cagatggtta ttattatttt acaggatcgg ttccaggcta ccaagtgatt
    13921 gaattaagaa gggcaaagtc actgaatgaa ttagaacatg ctgcaagcct gattgtatgg
    13981 caggctcatg aagaaggacc gatgagtgaa ttgatctggg cacctgaagt ccattatatc
    14041 aatggaaaat ggtatattta ctttgctgca agcaatcata aaactatacg tgatgaatct
    14101 catcatcacc gcatgttcgt acttgaaaat cagcatatag atccaatgaa tacagattgg
    14161 atagaaaagg gacaaataaa aacgaaaagg gaaactttca gtttagacgg aacaactttc
    14221 tcttctcaag gaaagctata ttatgtgtgg gcccaacaag atcctgaaat tactgggaat
    14281 tctaatcttt atctatccga aatggataat ccttggacgt taaaggggaa gcagatcctc
    14341 ctttctatac ctgagtatga atgggaaaaa cggggttttt cagtaaatga aggtccagca
    14401 gtccttactc gcaatgggaa aatattcatt acgtattctg gtagtgcaac agatgaaaac
    14461 tatgccatgg gcctgctgtg ggctgatgag cacaagaatc ttttattaaa agaatcatgg
    14521 cataaattat cggagccagt attcgtttct tctgaaaaaa ataagcaatt tggtcctggt
    14581 cacaactcgt ttacggtttc agaagacggg tgctttgata ttttgatcta tcatgctcga
    14641 ccagagaaga accaaaaagg ggatccgtta gacaatccta atcggcacgc gaacgcgcaa
    14701 tgctttacgt ggaatgagca aggatttcca gaatttggtg aacctgttcc gtatgcctta
    14761 tgagcacaag aaaccttgat ttttgcttga ataaaccttg cagactcgtt atgatatatg
    14821 tacgtacaaa taaaaagggg caaggcaaat ggcaaaatat caagatattg cagaagagat
    14881 aaaacaaaag atccttcgtg aggaatacga agtaaataca accattccct cagaattcaa
    14941 gctgcaagaa atatatgacg tcagcagaca tacgattcgg caagcaatcg ctgtgttggt
    15001 aaatgagggc tatctgcgta aagaaaaagg gtcgggcaca tatgtagatg acagctataa
    15061 gaaagaaaaa acaccattga acagaacaaa aacgatcggt gtcatcacca catatctctc
    15121 tgattatatt tttccgtcga ttatccgagg aatcgaaaat gaattacgag aatctgggta
    15181 ttcattgctt ttagccagta cgaataacga tcacgaattg gagaaagagt gtttgaagaa
    15241 aatgattgct tatggggtag atggtttgat cgttgagcct acgaaaagta atcagtataa
    15301 cccaaactta gcactttatg ttgctttgag agaacaaggc attccgatgg tgatgatcaa
    15361 tgctgcttat gaagagttaa atgcctctta tatttgttta gatgatgtga aagtcggttt
    15421 tatggcagca gaatatttga taaaaaaagg acatcagcgt ctgttgttca tcacaaaaat
    15481 cgacgatctt caaggaaaat acaggatgaa aggttttatc agcgcgtgtg aacagtataa
    15541 tgtgcgtttt ttgacggaag atatcattac tttcaccacg gaaacacgag aaacaatcgc
    15601 tgaaaaaaca gtacaacggt tgaaagaaga acaagcttta gatggcatca tttgctataa
    15661 cgatcaaata gctagtttaa tgctagataa gttgacagaa aatggctatc atattccaga
    15721 tgacctatcg atcgtaggaa cagacgattc ttctttaagc catttaggga ataaaaaatt
    15781 gacaacattg acgcatccaa aagaaaagtt gggacaagat gccgcacggt ggatcgtcac
    15841 aacgattgaa aaaggacagg cacagccaaa tattctctat gaacctgtac tagttgaacg
    15901 ggaaacagtg aaagaaaacg aaaaataaga gacgggtttt taccgcctct tattgctcct
    15961 acggttactc gtcgcaatgt ttgtgagagc ttttgtcatc gctcaaggct gaacgtttct
    16021 ttcaccacct ctccactaaa cggtgttctc ctaccaactg gtcacaagtc acaatattca
    16081 tcaaacatga gaagctgttt tttgaaattg ttccttgtga ctggaaacta agcggttcct
    16141 tcttatttct tgaagctgac cacttctgtc acaacctctt taagaatgat ggatagaaat
    16201 acgtttagga taagtttttt tagatgcact ttgttcgatt tcctctaaag atttcccacg
    16261 agtttctggt acgatctctt gaataaataa aacccctaaa aagcagatga tcccaaaaat
    16321 gccgaaaacg atttcttgag acatagaagc agtcatcaca ggaaaaagca agccaactag
    16381 gaaagaacca atccaattaa aagaagaggc taggccagat gctcgcccgc ggacagctag
    16441 tgggaatatt tcgcctacga ttacccatgt cagtggagcc catgtgcaag aataaaatgc
    16501 gacataaata cataaaaaca agagaatcaa caacgaattt gtatgagcat ccagtacagt
    16561 tcctaaaaca gctggtagga tgaaagaaag tcccatcacg ctaccgccta ttttcaataa
    16621 agttcgccga ttgaacttgt ctgcaataac caaaaagagc aaagaaccag caactaaaat
    16681 gattccttgg atgatcggcc acatcaatgc atcgctcgct gcatgcccag tcgctttttc
    16741 tacgatcagc ggaatatagt aaaaaatagc attagctcct tgaaattgct ggaatactgc
    16801 tacaccaata ccggcaatga ctaaggaacg atatttttct aaaaacaaag tatgccaaga
    16861 agttccggtt gtcttttctt gtttgacatt cagctggatt tgttcgaatt cctgttcagc
    16921 ttcttttggt gtccgaatat agttcaatac tctttttgcc tcttccaagc ggccagattg
    16981 cataagaaaa cgcggtgact ctggtagagc taaaacaccg aaaaataaaa tcagcgcggg
    17041 gacagcagcg agccctaaca tcacgcgcca agccatcgtt tcaggcaacc cttttagcaa
    17101 ataatctgca acataggaaa ggagcatgcc agaagcaatc atcacttgat tgatccctga
    17161 caatcttccc cttagtctag caggagccat ttcagacata taagcaggaa ctagggcaga
    17221 tgcagcgcca acagctaaac ctagaagaat ccttgctgca atcagaaaat aagaaccatt
    17281 gtgaggggca gatgcagaaa ggacagaacc agccataaaa ataagtgcag aaagaagaat
    17341 catttttcgt cgtccaagct tatcagaaag aagaccagat aaagagccgc caaggatcgc
    17401 acctaacatg accgaagacg tgatccatcc tatcaaagaa gctttacctg ccaaacccca
    17461 atcatgctgt aaaaaaggta aggcgccggt cataacacca atatcatatc caaatagaat
    17521 gccgccaaat gcgccaaaaa aataaataaa cgatgaggaa actttttttt cttttttcat
    17581 aagtattctc tccttctaga atttcgagaa ggcggttgat cagattttgt aagcaatttc
    17641 attttataac ttatgtacgt acaaaacaag tgttttttat tgtattcttg taaataactg
    17701 cttgacatgt acgtacaaaa gtatacaata atcattgtaa gcgctactaa aaagacggtt
    17761 tgatcaggtt tgacgagaag gagggagtta aatcattatg aaggaagtaa atgcgcaaag
    17821 tatgttagat gtcatagatg gtcgtacatc tttagggatt gagtttggat caactaggat
    17881 caaagctgta ttgattgatt catcttttac tcctattgcc gctggtagtt atgagtggga
    17941 aaacaaacta gaaaatggta tctggaccta ttcattagaa gatatttgga aaggactgcg
    18001 cgtcagttac cgaaaaatgg cagcagaagt attcgaaatc tatggcgaga atcttacgac
    18061 aatcgggtcg attgggttca gtgcaatgat gcacggttat ctagcatttg atagcaagga
    18121 acagttacta gtaccatttc gtacttggcg aaatacaatc actgaagaag cagaacgaat
    18181 attaacggaa aaatttcaat acaatattcc gcagagatgg agtatcgctc atttgtatca
    18241 atctattttg aataaagaag aacatgtgaa agaaattaat tttttgacaa cgcttgcagg
    18301 ttatgtccat tggcagctta ctggtgagaa agtactaggc gtaggagatg catcaggcat
    18361 gtttcctatc gatccttcaa cgaaaaccta ccatcaagaa atgaaaaata tttttggtca
    18421 gttaatcgaa aaagcgcacg tagcgattga cttggatcac attttgccta aagttctagt
    18481 agcaggagaa gaagctggca agttaagcga atcaggggct tatctgatcg accctacggg
    18541 aaatttgaga ccaggaatcc cggtttgtcc gccagaaggg gatgcaggaa ccggtatggt
    18601 cgctacgaac agtgtagcca aagggacagg gaacatttcg gctggtactt ccgccttcgc
    18661 tatgatcgta ttagaaaaag aactagaaaa tgtccatccg gaaatcgaca tggtcactac
    18721 accttcagga gaactagtag gcatggttca tacgaataac tgctcttcgg atatcaatgc
    18781 ttgggtcagt atttttgaag aatttaccaa ttctttgggt atggagataa gcagagataa
    18841 attattcacg gtattattca acaaagcgct agaaggcgat acggattgcg ggggattact
    18901 ttcttatggt tatttttccg gtgaaaacat tacaggagta aatgaaggaa ggccattatt
    18961 tgtacgtaca ccaggtagcc gttttgattt agcgaatttc atgcggatcc atttagccag
    19021 tgcttttggt gcaatgagaa tcggaatgga tatattgaaa tctgagaatg tccaaattga
    19081 tcgtttagtg gggcatgggg ggatttttaa aacaccagaa gttggacagc ggattctagc
    19141 atctgctatg gaggcaccag ttacagtgat ggacacagcc ggagaaggtg gagcgtgggg
    19201 aatcgcgtta ttagcagcct atatgatgga tcgacatggg aagtctttag aaaacttctt
    19261 ggcagaagac gtgtttggac aagaccaagg aatcaccatc acaccttcag cagaagaaat
    19321 agcaggctat cagatattta tgagacgtta ccgtgagggg atcgacattg aaaaaacagc
    19381 gattcatcaa ttgcaagaaa aaggagtgat agaaaatgct ggaacaactg aaagaagaag
    19441 tataccaagc aaatcttgac ttgcctaaac acggactcgt caaatataca tggggaaatg
    19501 tgagcgcttt tgatcctgaa acacgatatt tcgttatcaa acctagcgga gtcagttatg
    19561 aagagatgac agcagacgac atggtcgttg tggacttgga caatcatatc attgaaggta
    19621 aactcaatcc ctcttcggat acgcctactc atgctgtttt atatcgttca tttcctcaaa
    19681 tcggaggaat cgtccatact cactcgacat gggcgacgat ctgggcacaa gcaggattgg
    19741 atgttcctgc gatggggacg acgcatgcag atacttttta tggatcggta ccttgtgctc
    19801 gatttttgac acaacaggaa atcgacagcg ggtacgaata tgaaacagga aaagttatca
    19861 tagaaacatt caaagaacgc aaaattgatc ccttagcaat tccaggggta cttttacacg
    19921 gacacggtcc atttacttgg gggaaagatg cgcaaagtgc ggtaatgaac gcagtcgtac
    19981 tagatgaagt atgcaagatg aatttattta ccagacagct caattcattt tcagaagagc
    20041 tgcctcaacg gattttagat aaacattatt tacgaaaaca cggaaaaaat gcgtattacg
    20101 gacaaaacaa ataaaaggag tggctaatta tgttgaatat tggagaaaaa gaattttggt
    20161 ttgttgtagg atcacagcat ttatatgggg aagaagcact gcgagaagta aagaaacatg
    20221 cacaagaaat ggtcgatgaa ctgaatgaaa atggccaatt gccttatcca atccgccttc
    20281 aagaattagc agttactgct gatacgatca caaaaatcat gaaagaggtc aattatcgag
    20341 aagaagtagc aggtgtgatc acttggatgc atactttctc accagcaaaa atgtggatcc
    20401 gcgggacaaa gcttctacaa aaaccattgc ttcatttagc tacgcaatat aacgaaagca
    20461 ttccttggaa aacgatcgat atggatttca tgaatttaaa tcaatccgct catggtgacc
    20521 gcgaatatgg gttcatcaat gctcgattaa ataaacaaaa taaaatcgtc gttggctact
    20581 ggaaacgacc tgagatccaa aaagagattg ctgattggat ggatgttgca gtagcctata
    20641 atgaaagttt tggaataaaa gtggctcgtt ttggtgacaa catgagaaac gtgggtgtca
    20701 cagaaggaga caaagtggaa gcacagattc aatttggttg gactgttgat tattttggta
    20761 tcggagatct tgtacaagta atcgatcgcg tatcagacga agaagtcgat caattatttg
    20821 aagagtacaa ggaattgtat accttcgact atggtgatta tgaagaaaag acatgggagg
    20881 aacatgtaaa agtacaagct cagcaggaaa tcggtattcg ccgtttctta gaagaaggag
    20941 gctataacgc ttttacgact aattttgaag acctgtatgg aatgaaacaa ctacctggac
    21001 tagctgtaca acgtttgatg gcagaaggat acggttttgc tggggaaggc gactggaaaa
    21061 ctgcagcgat tgatcggttg ctgaaaatca tggctcgagg gaaagataca ggattcatgg
    21121 aagattatac ttacgagcta gctagtggcc aagaagcaat cttggaatcc catatgatgg
    21181 aagtagatcc gacgcttgct gcgacgaaac caagaatcgt tgtttctcct ttatcaatgg
    21241 gagatagaga agatccggcg cgtcttgtat ttgatgggaa agcaggagaa ggggtagtcg
    21301 tatcgatggc agatttcggt acgcattata aactgctgat caatgaagta gaagcctttg
    21361 aaccaacgac tgaagcacct aatttacctg ttgcacgtgt gttatggaaa actaaaccga
    21421 atttccatga aggtgtccat tcatggattc aggcaggtgg cggtcatcat accgttgtct
    21481 cgctaaatct aacaacagat caaattgaaa cgtgggcaaa attagttgag ttggaaacag
    21541 tggttattag ataaaaagag gttgtgaaaa agccacttag ttttaagaaa taagaagaga
    21601 ttgacgaaat ttgcttttca acttttttga aatttagtct tattatcaat gtcagctaac
    21661 ggaacaccat ttatcaagat atctcaccct caatattttg ctctcgaaaa taaggcttat
    21721 ggcagtaact tgtcatagcc ttatttttat cactcagcct actctgctct aactgctctt
    21781 tttgcttcta ggaagtcaaa tggtaaacag ttgtctaatt gcagaaattt cgagatactt
    21841 gttagtaagg agggggtttt atgtatcacg ttcatttgat tgatgctatt ccagaacaag
    21901 agatagaaaa gttacaaggg gaatatttca aaataataga tgatttgcca gccgaaggaa
    21961 tagttattcg tagtacgaaa gtgaaagatg aatggcttac accagatttg ctagcaattt
    22021 caagagctgg tgttggggtg aacacgatca acgttgaaaa agcttctgaa aacgggacga
    22081 tcgtcatgaa tacaccaggt gtaaatgcta atgcagtaaa agagcttgtt ttgtgctgtt
    22141 tgctcttatc ttcccgtccg ataatcgaag caagtcgtat ggtacaaaca ctaacaggtc
    22201 ctaatatttt agaacaggca gaaaacaaaa gaagtgctta tgtgggacga gaattgcaag
    22261 gcaaaaccat cggtcttctt ggtcttggag cgataggcac aaaagtagcg ctaagttgct
    22321 atagcttagg gatggatgtc ttgggttatt ctatccgcga tgcccagttg gattatgttc
    22381 ggcaagcaga tttagaaaca gttctttcta cctcggatta catcgtagtc atgctgccgt
    22441 tgacagaaga tacaaaagga ttgatcgatc aggcgaatat agaaaaaatg aaaaaagatg
    22501 ctgtacttat caatgtagga agaagtgaaa ttgttgataa atatgctgtt atgcaagcat
    22561 tggaaaaaca acatctggct aaatacttga ctgattttcc agaagaagag ttcttagaaa
    22621 atgaccgtat acttatgttg cctcatctag ggggatcgac tcaggaagct ttggcagaca
    22681 gcggacgttt ggcagttgaa gccttaaaag attatctttt gttcggtacg gttagagaag
    22741 cggtcaatta tccatccgca cgaatgtttt tccaagcgcc gtttcgattc actatatttt
    22801 atcaaaagaa aaccaatata ttagcagaga tattttctct gctgaatgag aacgagctag
    22861 cgatcgcaga tatatcgcgt aaccataaga atgagcatgt ctacatattg attgacatag
    22921 acagtactga ttttgaacag ctcaatcaag tgaaacaaca gctagaaaaa atttctggag
    22981 tacgaaaggt ccgcttatta aagaaaccag aaaggccata aagaaagaga atatttggat
    23041 ttcattaatt cttctttccg ttctttactt tcgaatttcg tacaactaaa ataagagaag
    23101 caacattctt ttattgatag aaatgaagga gcaagcattt gaaaagatat ataagcagac
    23161 tttcgaccaa acagaaaaag attttaaaag gaacagcagc aatcatcagt atcttttttc
    23221 ttgttgtttt tagtaattta tttttgcagt ggtgtcaaaa tgatctatcc gtagaccttg
    23281 cgttgaaatt tgctttttct tggcatacgg aaaaattctt cttagcctgt ctcgtcttgc
    23341 tgattattct tatttttctg attgcactag caggatctgt ccctttaggt tcccttacgt
    23401 atgtcgtagc catcggtgta ttaggttttg caaattatat gaaaatgagt tatcggcaag
    23461 aaccgattta tccagacgat ttgaagatga tcactgaaat cggattgctg aaagatatga
    23521 ccggcactat gctgtttaca gtgatcttag ctgccgcagg aaccgtgata ggattgtttt
    23581 gctggtacat gtttcgaagc ctgaaaaaag gaaggcgatt ccaattgatc cgattaacga
    23641 cactgcttgt ggcaattgga ctgctagggt atatcagtaa tttcaacaat cccgataatt
    23701 tgttgcgaaa agcatataat aaaacggcac tatggatacc ttacagccag aaaatgaact
    23761 actataacac tggttttatc ggtgggtttt tgtacaatct gaaggtagaa ccaatggatg
    23821 aacctgaagg atattcgaaa gaaaagatca aagaaatcac tgaaaaatac cagaagctag
    23881 ccgatgaaaa aaataaaact gtagaggaag agtcgccgaa catcgtgttc gtgatgagtg
    23941 aaagtttctc agatcctagt cgattaaacg gcgtagaagt atctggtgag ccattggcag
    24001 attattatga agtagctgat caaacatata gtggcaatat gctttctcaa aactatggcg
    24061 gtggaacagc aaatatcgaa tttgaagcat tgactggttt ttctatggcg ttatttaatg
    24121 cgcaactcac caccccttac acgatgcttg taccaaaaat ggatcagctt ccttctatcg
    24181 tttcagcgtt aaacgcacaa tcataccaga cgacagcgat ccatccatac aatacttcga
    24241 tgtataaaag agaggatgtt tatcagacat taggatttga ccagttcatc agcgagcgta
    24301 cgatgaccta tacggatacg atagagaata atccatatat ttctgatgag tcagcttaca
    24361 aagaagtaat gacacttctc aaggaagaaa aaacacctca gttcattcat ttagtcacga
    24421 tgcagacaca tatgccctac gatggaaagt atgataatct agactatcct gcaaaaactt
    24481 cagatggaag tgggacactt gacttagaga attatctgca agatatttcc tatagcagca
    24541 cggcactgaa gcagttcacg gaagaattga agaatctttc tagaaggact ttagttgttt
    24601 tttgggggga tcatcttcca gggatctatt cagatacgat ccaagcaaaa aatgacaaac
    24661 aaacattgca tgaaacacaa tttcttatgt tcgacagtaa aggagaatta gaaaagcaaa
    24721 cgactcacga tgcaatcacc agtccgtttt attttgcagc gaatctaatg gaacagacga
    24781 atcagacaac aaatggcttt tatcagctgc ttttatcatt agaacaggaa cttccggcgt
    24841 ttgaacgaga actgtactat caaaacggac agtggtataa agaagcgcaa ttcaatcgct
    24901 cgcaacaaga gatttacgac gaatatcaat tgatccaata tgacattgtc gcagggaaac
    24961 aatatagttt agcagatggt ttctttgagc acgaataaga atggcgagct gtatagaaac
    25021 cattaaattt tttcaagtac ttgtcatttg aggataaaaa caagtagaat aaataataac
    25081 aacaatggag aaagtaggaa aacgaacatg ttaccgaata ttggctatta tgtgcagtcc
    25141 atcgatcaaa tcgtcaagga aacagaagca atcggagaag gaatgcatcc gaaatatgaa
    25201 gtgatcagaa acgcaattga tgaaggaaaa acaagtgaac tggacaaatc gactcttgaa
    25261 gagatcatcc tgttatttga agaaggaaca aaaaaatatc gggcaatgct tatgaaagtc
    25321 agtactttgc gtccaccagc aaaagcaatg ggcattcata aaaattttga aaaagcttat
    25381 ctttcatatg tagcaggctg tgaagaaatg atccaaagta tcgatcttga atcaggggta
    25441 gatgaagatc tattcaatac atctgaagca aaacaagacc aagcgacaga ccaattgaca
    25501 gctgccatcc aaaaaatgac taatttgttg ttgaaaaaat aaaagaaatc tttgataaca
    25561 ataaaaacta gagttatcta atataaatgt aatattacct ttatgataga gtgatcaaaa
    25621 tcctttatga taatgacata cctataacca acaacaacct tgtacttact caaaatcgag
    25681 taaatactcc ttttccgtcg agtctaacca tctcggcggt tttttatttg tagaaaagaa
    25741 catcaggata gacggaaaag tcttgtcctg atgttctttt cagatagtat ggaaagctga
    25801 ttagttttct acattctgta catattgttc aaagtagttc tgtaaatcgg ctttgagttt
    25861 ctgatattcc tcttcgctat actctttatc ttcgattttc ccatcaaaga aaggaatatt
    25921 tagttcttcc cgtagtctgt ctaatacgtc ttgctgattc atttcattct ccttttctta
    25981 ctaacgatca aaataaacac agacacattc tggtacatag acatcttttt tcagtaattc
    26041 cagtaatcct gtttcaggat gacggcgata taatgtcaga ttatcggtgt tctgattggc
    26101 acacacgaca aaattattgc ttggatctag tgcgaaatcg cggggaaaat ccccttctgt
    26161 tgagatgatt tgcttgtttt gaatgtggga accatcttca aaaactgcga atacagcaat
    26221 cgagttatgt cctcggttag aagcataaag gaacttgcca tcatttgaaa tgcggatagc
    26281 tgcccctcca ttttcaccgt cgaattcttc tggcaaggtc gatattttct gtaattcttc
    26341 aaaagcacct gtttctttat tataagaaag cacgcggact gtgctgtcta attcaccaaa
    26401 taaataagcg aattgtccat ttggatgaaa gacaagatgg cgagggcctg ttcctggctc
    26461 tgcagtgaag gtagttacaa gattcaattt gccgttttcg gacacatcat aagtatacac
    26521 acgatctgta ccaagatcac atacgactaa tcgttggtca ggagtcagat ccgtatagtg
    26581 gacatgcgca tgatcttggt ttttatgagg acctgtcgct tcttgatgga atattgaatc
    26641 tgctgctttc agttcgccat ctcctaatat atggtagaca tttacttcac ctttatgata
    26701 gttcgccccg tatagtaatt gacgtgcttc atcaaccgct acataacaca aaggagcgcc
    26761 agactctgtt accgcattca aaaaatgaaa atccttatcg tacgcaccgg caccacctaa
    26821 tccatccaca ctagtaactg tgtaaagatt gccagcttta ctttttgcta cataggtagg
    26881 gctattttct tcaacagcta atgaaaggtt ggctaattct tctttttctg tatcaaggtc
    26941 gatcgtatag attcctttac tttcgcgacg cgtataggtt cctaaaatta tcttttcgat
    27001 catatgagct gattcctcct aattgtaatg aaatagtttt cgtttttatt ctatcacata
    27061 attattccac acggctaaag aatgagaatg tgttatactg tttttgaaag caattaaagg
    27121 agagagtgca atgattcagg caaagattac agaaattggg acagaagcaa taaacaagga
    27181 agaacccatg ctgattttgt tcgataaaac agcaacagct gccttacgaa aatattcggt
    27241 gattcaagaa gtatcaaaag acgaaccttt ttctttgaaa gcaggcggaa caattgcttt
    27301 tgatgatcag gtatatacga tcgattatgt cggtccgatg gccaatggga atttaaagac
    27361 agtagctcac gtatcattga tttttgatgc ttgtccagaa gaaggacaaa ttgcaaatgg
    27421 aatctatctt catccgcata gactgcctgc tattggtttg ggaagccaaa ttttttataa
    27481 ctaacaggag gaattatgga aggaaaaaat aagaagaaca gaccggtatc gtggttttgg
    27541 cgctggtttt taaataatca agtagtcaca gccttacttg ttgtattgtt aatattgctg
    27601 atcgtctttt tatttacaaa agtttcatat ttgtttgcac caatctggca atttttagca
    27661 atcgtaggtc tgcctatcat tttagcagga atcttgtatt acttgatgaa tccggtagtt
    27721 gattactttg aaaagcgaaa agtaccaagg ctttacagta tcattgggct gtttgttttg
    27781 gttgttgcac taatcgcatg ggggagcgta gtgatcatcc ctaagattca ggaacaaaca
    27841 gtgagcttca tcagtaactt ccctcaatat gtagatacga ttgataataa attaaccgag
    27901 attttgcggg acccgctgtt caaccaattt cgcgaacagt tggaaacagc aggtgataag
    27961 ttcatgaatt cggcaggcga tatgatccaa gatatatcta agagtacagt tcagagtctt
    28021 ggaagttttg tcggcgcggt agcgactatt ttagtggctc tgctgacgat gccgtttatc
    28081 ttattttatc tattgaaaga cggtaaacaa ttagcacctt attttgtcaa atttttaccg
    28141 acacgcatgc aaaaaccgac gttgaatgtg ctgagtgaaa tgaatagtca agtttcttct
    28201 tatataagag gacaactaac tgtggctttt gctgtggcaa tcatgtttat gattggtttt
    28261 gcggtcattg gtttagagta tgctgtcacg ttggggatta ttgcgggttt tttaaacctg
    28321 atcccttact taggatcatt tttagcgatg attccggcta tctttttagc cattgtagca
    28381 ggtccattca tgatcatcaa agtactgatc gtttttgcag tagagcagac aatagaagga
    28441 cgcttgatct ctccattagt actagggaat cagttagcaa tccatccagt aacgatttta
    28501 ttagttctgc tgacttctgg gaaattgttc ggaatcatcg gtgtgatctt ggggattcct
    28561 gtttatgcag cagctaaagt agtgattact catgtttttg aatggtacac aacaatctct
    28621 tctctttatg aagaaaatcc gttgaaaaag cagaaagaat aacaaaaaga gacagatggg
    28681 aaaatatcca aggcacaaat gctgctttga agattttttc ctctgtctct tttgtatgca
    28741 tttatctcaa tttttaaaat gatttaccta aagcagcagc tttatctaat gcattttgca
    28801 tcaactcatc tgcacggtca gggtcataat cgattccttc gatgaatagc tgatctactt
    28861 gatcgatgcc tacaaagttt aaaatacctt ttacatattg agaggcaaag tcttgtccgt
    28921 tgtatacacc gccattagat tggatgtgca gtgctttttt acctgtcact aatccttctg
    28981 gcccgttttc tgtatagcgg aacgtttttc cagctacatt gacggtatcg aaccaagctt
    29041 ttaattttgt tgggatattt agattccata aggcgtttgc gataacgatt ttatcagcag
    29101 caaggaactg atcagtcaat tcattgaaac gagcaatttt ttgttgttga ctttctgata
    29161 aggaagtgaa ttctgcacca gcacgtaatg cttcccaacc tgataataat tcttcatcga
    29221 tttccggaat atcatcgcga taaagttcca aaacagtcac ttcatcatct ggatgatttt
    29281 ctttataact tgttaaaaaa gacgtcaatg ccttaactgt gcgcgattct tctgcagtta
    29341 ggggatgacc tttgactact aaaacagatg ccatatgctt accttctcct tttattgcta
    29401 tgattgttat aactatgact aacaaaatca gtttactcga aaaagaaaag gctatcaagt
    29461 catattgata aggttgaaga ataaataact aaaaaaagac agggtataaa agaggctgtg
    29521 acacgatttt ttgtcacagc ctcttttgaa tctaacaagc aatattcaag caacactcga
    29581 ctgaagagct tccatcccac caatgatata aacagcacga aaaccattag cagtaagttg
    29641 atcggctaat gcttctgatc gcctgcctgt atagcttaga atataataaa gtttctttgg
    29701 atctagattt tttaattgtc ttggcaagat agcagcgggg atagaaagcg agtggcttaa
    29761 gtggtttttt gtgtaatcta aggtatttct tgtatctaaa acgatgctgt cagcatcgag
    29821 aggcaattta ttgaagtctt ggacactaat tgattgggcc atttatctca tcctttgttt
    29881 agtatgtggc tattttaatg ggcaactctc attaattcaa atggaaggat tcctcttatc
    29941 acttttttaa tgtatcggat gataatgaaa aaaactatgc aaatgtcttg caaaacaggt
    30001 aggaggtgtt ataatgacat agcgtcatat gacaatgtgt catcttgaaa gaaaggagaa
    30061 tccatatgcc gacacagaca tttttccatt tgcctaaaga aaaacaaaaa agactaatcg
    30121 aggctgcgag aatcgagttt tctagagtac ccttaaaaga agcttcgatt gctaatatcg
    30181 tcaaattagc ggatatccct agaggcagtt tctatcagta ttttgaagat aaagaggatc
    30241 tttattttta ttactttgag acaatgagac gtgacagctc taaagatatg ttacaactaa
    30301 tgaaagaatc cgatggtgat ctattcaaag gattcgaatc ctatttttct aaaatgattt
    30361 atgagatttt ggaaggcgag aatgcttctt tttatcgtaa cttgtttatg aacatggatt
    30421 accgtgcctc aagaaaagtc gctcctcata cgagtcgcca aaaaacgcct cacgatccac
    30481 ataaagaaca caaacaagat gcaaaagagt tgcttgaatt gatcgatcgg acgaagctga
    30541 aagtaaaaaa tgatcatgaa ttggaattac tgatccaatt agtgatgaat actgtattta
    30601 caacaattgc gcattcgtat cgcttatttt caacatctga agattactca gtagagcaag
    30661 caatcagtga attcaatttg aaactttctt ggctgaaaaa cggagtatat aaatagaaag
    30721 aagggaacta gatgctaaaa atcgtaaaac ggatctcctt gacttcagca attgcagctg
    30781 cagttttcat ggtgatccaa gtactggcag atttatattt gccgacactt acctccaata
    30841 ttattgataa aggggtagcg caaggagatg tcgattatat ttggcataca ggttttgtga
    30901 tgatcggctt ttctttgatc agtatcttag ctgctatcgc aaatacattt tttgctactc
    30961 gtgaatcaca gaaactgggt aagcaattgc gtacggatgt ctataaaaaa tcagagagtc
    31021 tgacaaaaga tgcgtttgat aaatatggaa cggcctcttt gatcacaaga acgaccaatg
    31081 atgtgactca aatccaaatg gttacacaaa tgtttttacg tatgatgatc aatgcaccaa
    31141 tcacgttgat tggcgcgagc atacttgctt atcaaaaaga tcatcagttg acaaaaatct
    31201 tcttagttgt catcccagta atgattattt tgatcggcgg aatcatgtac tttgctgttc
    31261 cattattcaa aagtatgcaa aagaaaactg accgtttaaa cctggtattt cgagaaggat
    31321 tgacaggggt cagagtgatt cgagcattcg ataagacacg ctttgaagaa aatcgttttg
    31381 atttagcgaa taaagattat acaaatacgg caatcaaagt taatacgatc gtcgcattga
    31441 tgatgccaat gatgacattg atcatgagcg gtacaaacgt agcaatcact tggtttggtg
    31501 gacactacat agcagatatg actttggaag tcggtaatct gattgcattt atgacttatg
    31561 cgatgcagat cttgatcagc tttatgatgc tttccgctat ttttatcatg gtgcctcgtg
    31621 cacaagcttc agctgaccgt attaatgaag tgttggatga aaaaatcggt atccatgacc
    31681 ctgaaaatcc taaaacagtt tcttttgctg gaaaaaacgc aacattggcc ttcaatcatg
    31741 tcaattatcg atatcatgga gcagaaaaac ttgcattgga agatattgat ttccaagcca
    31801 agtcgggcga gatcgtcgca atcatagggg ggactggttc aggtaaaacg accttagtca
    31861 atttgatccc tcgattatat gacatcgaat ctggttcgat ccaaataaat ggaacggaca
    31921 ttagtgacat gactcaatat aatttgcgag aattaatggg ctttgttcct caaaaagcag
    31981 ttctgttctc tggaacgatc cgagacaata tgcagtatgg taagccggat gcaacagatg
    32041 aaatgatttg gaaagcatta gaaatcgcac aagctaaaga ttttgtttca gagatggaag
    32101 atggcttaga tagccatgtg gaacaaggag gaggaaactt ctctggtgga caaagacagc
    32161 gtctggcaat tgcacgggca ttagtcaaaa cagcagatat ctatgtgttt gatgattcct
    32221 tctcagcgtt ggatttcaaa acggatgcca atcttcgcca agcattgaaa acgaatatga
    32281 aagaaagtat caccgtttta gttgctcaac gagtaagtac agtaatggat gcagatatga
    32341 tccttgtgct agatgaagga aaactggttg gcaaaggaac acatgaagaa ctattagcaa
    32401 caaacgaaac ttatcaagaa atcgttcatt cacaactaag agaggaggac ttagcatgag
    32461 taaaacaaac acacgtccct ctcatggacc agcaggcggt ccaggacatg gtggacctgg
    32521 cagaaatctc ggtgtaaaag gtgaaaagcc taaaaatttc tggggaacgg tacgccgatt
    32581 gtttggctat atgtctaaac gtatggttgc gatcatcgct gtatttgtac tagcaatagc
    32641 agccgtgatc ttccaaatcc aaacaccaaa ggtcttgggg aaagcaacca cagaaatatt
    32701 caaaggtgta atggctggcg ctcaacagat gcaacaaggc caacaagtaa caaaatttcc
    32761 gatagacttt gataaaatcg cgcagatcat tgcgacagta atcgtgatgt atttgatttc
    32821 tgctgtattc aactttttac agcaatttat tatgacacgt gtttcccaac gaacagttta
    32881 tgaattacgt catgatttgg aagccaaaat gaatcgtgta ccgatttcct attatgatac
    32941 acatacaaat ggggatatca tgtctcgagc aatcaatgat atggataaca tcgcaagcac
    33001 gctgcagcaa aatttgactc aatttgtaac tagtgcagtc acattgattg gggtcatcgt
    33061 gatgatgctt tcaatcagct ggcaattgac gttagtcgcg ttattgatga tccctttaag
    33121 cttgatcatc gtgatgatcg tagcgccgaa atcacaagtg tttttcgcag atcagcaaaa
    33181 aagtttaggt ttattgaata accaagtaga agaaacatat ggtggtcata ccattgttaa
    33241 aacattcaat catactgaaa aagaccagga agtatttgaa aaagaaaatc agaatctgta
    33301 tgccgctggt tggaaagctc aatttatttc ggcaatcatc atgccgttga tgaatttcgt
    33361 taaaaatcta ggctatgtgt tcgtcgctgt actaggcgga gtaaaagttg caaatgggaa
    33421 catgacatta ggggatgttc aagcgttctt gcaatataca acacagttct ctcaaccaat
    33481 cactcaactt gcaagtttga tgaacactat ccaatcaaca gttgcttcag ccgaacgggt
    33541 atttgaagtg ttggatgaag aagagatgtc caatgaaaaa tctggccttc cagaagaaaa
    33601 agatacacca tacaaagttc gttttgaaaa tgttcaattc ggctacacac cagataactt
    33661 gctaatgact gattttaact tggatgtcaa acctggtgag atggtcgcta ttgtaggtcc
    33721 gactggtgca gggaagacga cactgatcaa ccttcttgaa cggttctacg atgtttctgg
    33781 aggtagtatt cgttacgatg gtgtagatac ccgtgatctt tcacgtgatg aattgcgcgc
    33841 acagttttca atggtactgc aagatacttg gctgtttaca ggaagtatct atgacaatat
    33901 caaatatggg aacgacgatg caacagacga acaaatcatt gaggctgcta aagctgctca
    33961 cgtagacgat tttgttcgta aattgccaga aggatataac actgtattaa acgaggatgc
    34021 aagcaatatt tcgcaagggc agcgtcagct aatcacaatt gcgcgggcat tcttagcaaa
    34081 tccagatgtc ctgatccttg atgaagcaac atctagtgta gatacacgaa cagaaatact
    34141 gattcaaaaa gcgatgaacc gcctattgga aaaccggaca agtttcgttg ttgctcaccg
    34201 cttgtctaca atacgcgatg cagacaacat tattgtgatg aatcatggtt cgatcgtaga
    34261 aacaggaaac cacgatacat tgatggaaaa agacggattc tatgcagatc tttataacag
    34321 tcagttttct gaagaagaag cttcatagaa tagtacaaat ttattatttt ttttaggggc
    34381 tgtgacaaaa gtcgtgtcac agtccctttt tccgaataaa cggtgttcaa tcagctgtcc
    34441 ttcggtatta gctcaaaaaa cggcaaaata tgagaagcta tttttccgtt ttgtatctct
    34501 aatactcagg tcaaaccgct gattttacaa cctcttcctt aaacggtgtt ctcctgccaa
    34561 ctcctcggtc acaagtcaca atagtcaaca aacctgagaa gctgtttttt gaaattgctc
    34621 cttgtgactc ggagttaaac ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc
    34681 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
    34741 ttcttactac tcgaaactaa gcgacttttt cacaacctct tccttaaacg gtgttcaaaa
    34801 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg
    34861 gctgttctta ttacttgatg cagaacagct ttttcacaac ctctttttat aagagttttt
    34921 actcattaat ttacaaaaaa caagcaaaat gcgaattttc tcacattttt tttaacaaaa
    34981 tcaaagttat tgcttgaaag aagaagaatc catcgttaaa ataaagcttg acgtagaggg
    35041 gagcaagaaa tatgaaagat tttcaattgg gggctgccac ctatttaaaa agtgtagcca
    35101 ttcgggtgaa agaccgagat aaaatgattg atttttacaa gaacgttgtt ggctttgatt
    35161 tgaaaagaga agaaaacgaa ttagccattt taggaacaga agaagctgga agtgaaatgt
    35221 tgctgctgga agaaagtcct agagcgaatg atcacatggg ggtgatcaaa aagctgaacc
    35281 gtttttcatt gattatccct agcacagaag aaatggcaga tatcctttgt cgtatccgta
    35341 aaatgaatta tccaatcaaa gaagctttag aagatcatgg acgtatgggg attttgctcg
    35401 tagacccaga agagaatgaa ttggaaattt accatgaaga gaataagaac gaccaaagag
    35461 aaattccaga aacaatggat caagaagcat tattaagaaa atctgaagaa aaattcagta
    35521 aactatcaac aggttcttat tttgaaaaaa tccatttaaa tgttactaac ttaacaaaag
    35581 aacgccaatt tcttaatcat gtacttggtt tgaaggtaca taatgaagaa gagggcatcc
    35641 atgtattgaa tgaaggagat ttccatgttg gtttgacaga agctagcgga ggcgcaattg
    35701 atttgcctac agatgaagtg attggacttg attttctgaa attcatgatc gatactgaaa
    35761 gcatggaagg actgattgct cacttgcagc aactatcagt tgatttcttc ttagacaaaa
    35821 agcacacagt actcacaatc tatgatgcta taggaatcga atggtggttc gtcagtgagc
    35881 caaaagaaaa gaataaaact gacattcgat gcgacgctta actttttctt tcttaacgat
    35941 tgtcgcatgt ctcgtcattt tatgaacggt catactatcg cttattctag agatagaaag
    36001 agaaaatagt ataccggggg aaactgcgcg gtttttcgcg cagttttttt gtgtaaaaaa
    36061 ggttgattcc tcatagatta agctttaagc ttggactgta agaatctgat agaaaagcag
    36121 gagaaaaaga tgtatccaca cacgaaacag cttattttga aaaagatgga agaaggattt
    36181 tatccaggtg tagcagtcgc cttcataaaa gaaaatcaag tagaaacatt tacagccggc
    36241 tttgcccaag tcgttcctga aaagcagctg atgacagaag atctgttgtt tgatgcagcc
    36301 tcactgacga aagtcgttgc aaccacttca ttagttttac gtctattaga agatggaaag
    36361 atagaactcg atcaaccgct tcataaatat cttcctgaat ttgaaaatca gacaatcact
    36421 ttgcgtcatt tactgacaca tacttctgat atcaaaacat ggataccaaa cagagataag
    36481 ctatctcagg aagaattgat cgccgcttat ttgaagctag aaccaggtga gcaactagga
    36541 aaagaagtaa aatatacaga tgccggtacg attcttcttg ggttgatgct agaggaaatt
    36601 taccaagaaa ctgcagtgga aatctttcaa gaacaagtat tggaaccatt aggaatgatg
    36661 aacagcttat ttcttccttt tccttccaaa aaaatcgttc ccacagaaca actgccttcg
    36721 ggtgaggttt tgcgtggaca aactcatgat ccgaaagcaa gagttttggc tgaacatgca
    36781 ggtaacgcgg gtctctttat tgatttgaaa gacagcatta cttttgtcca aatgtatttg
    36841 aacaagggaa aaatccgtaa tggtcgtttt ttagaagaag agacgattga ttctttactg
    36901 caagatcaaa caccgaaaaa aaatggctat cgttctttag gatgggactt aaaaaaggat
    36961 gtagaagacg gtcatgcgat tctttttcat actggttata caggtacgtt tttcttgatt
    37021 gatataaaaa cacaagaagc gttgatattt ttgtcaaatc gcgtacatcc tgtcgatcat
    37081 cgtgcggagt atctcatatg tcgagatgaa attgttgaaa actttttgaa agagaaagcg
    37141 gtattaccga aataatgcta taatagctat gaaaaatgag aggagtttga attatgcagg
    37201 aacctatgtt tttaaaacca gtttttcaag aaaaaatttg gggaggaagt cgtttacgtt
    37261 cagtctttgg ttttgatatc cctaacgata aaatcggaga agattgggcg atcagtgcgc
    37321 atcctcatgg tgtcagtgta gtagaaaatg gcgaattcaa agggaaaaga ttagatgaac
    37381 tatggtcaga acacaaggaa ttgtttggcc atccttcaga accagtgttt ccattattga
    37441 tcaagatctt agacgcagaa gatgatctat ctgtacaagt acatccagat gatgcctatg
    37501 ggatgaaaca tgaaggtgaa ttaggaaaaa ctgaatgctg gtatatcatc gatgctgaac
    37561 ctggtgcaga aatcatctat ggtcatcatg caaagacaag agaagaacta gctgagatga
    37621 tcaaagatgg gcgctgggat gaccttttga agaaagtacc agttaaaaaa ggtgatttct
    37681 tttatgtacc aagcggaacg atccatgcta ttggtaaagg aatcatgatt ttagaaacac
    37741 agcaaagttc tgacacgact taccgcgtgt atgactacga cagaaaagat gcgcaaggac
    37801 aaacaagaga gctgcatatc caacaatcga tcgatgtaac aactgtccct gcgaaaacac
    37861 cagaattaca aatcaaagaa gtccgaaaag ggaattcatc gatcgtcact tatttagaaa
    37921 ctgagttttt caatgtatat gagtgggata ttaaaggaat cacatctttc aaaaaacaag
    37981 ccccttatac attgatgacg gtaatcgaag gagcaggaga ccttgtcgtt gacggaaaaa
    38041 catatccatt agaaaaagga acaagctgta ttattccaaa tggcgtttct gagtgggcgg
    38101 tccaaggtga actagcaatc attgcgtctg aaccaggcaa atagttccca gatagctgaa
    38161 agcagcagac ggtttttcgt ttgctgcttt ttctacataa aatccgtcca tcaataaata
    38221 gaagtctggt gaaaaagatg gaatacaaat ggaaagcaaa caacagcagt cgcgtgtcta
    38281 tgaaacaact gagaaaagct ggacatcact taaatgaaat gatcatgcca aatctaagtc
    38341 tgtttatcgc ttggggaatc ttgtcattag caacaaaagg tgtgtctggc gatttgcaca
    38401 ccacattaga agaggtatgc cattggatga tccagctgct tttacccatc ttgatcagtt
    38461 attcaggcgg gaaatcggta gggggacaaa aaggggcagt cgccggtgca attgcttctt
    38521 tagggttgat cgctcgttcc aatgctccac aaatcgtagg agcgatgatt atcgggccgc
    38581 ttgccggcat cgtatatgaa caatttgttt ctagattcca tgtgaaattc aaggctggat
    38641 atgaaatgct tgtcagcaac ctactagtag gactgactgg tagcgttatt tgtataattg
    38701 gaatcgtttt tattgaacca gttttaaata gtgttcactt gttgctcgcc tccgttgtgt
    38761 cttggctagt cacaatgaat ctgttaccac ttgtcagtct gatcttagaa cctctaaaag
    38821 tactattttt caataatgca atcaatcatg gtgtattgac acctttagga atcgaattct
    38881 cacaaacagt cgggtactct tccttatttt tgatggaggc aaatcctgga cctggtttag
    38941 ggatcttact ttctatcctt tgttttgcag aaaaacagga aaaagtaaat gcgagtggag
    39001 cattgatgat ccatgcgatt ggcgggattc acgaaatcta ttttccattt gtcttgctgc
    39061 gaccgtatct atttttagct gtaatggctg gtggggcaag cggcacgttg atatttcaat
    39121 ggttagatac gggattagct gcacctgtat cgcctgggtc actactcgtg atttttgcca
    39181 atgcgcctta tccacaacta gctggggtaa gtatagggat agcaatcagt acatttgtga
    39241 ccttcatttg tgcagcgata ctattgaaat taccaaataa agaaaaagag aacaaaacgg
    39301 tcaaagaaag gggagaaatc ttggaaccat ctatcattca ttccgtcatt tttgcttgcg
    39361 actcaggtat gggatcaagt gctatgggag ccgccctgct gaaaaagaaa ttagcagcag
    39421 aaaaaatcga tctttctgtc ggatacagct ctatctatga attgaataac caagtaaatc
    39481 agctggttat cgtgcaacat gaactagcgg gtcttgcaag agaaaaagta cctcaaagtc
    39541 aaatctatgt actggatcac tttttggaaa cagatatctt gctgtcacga ataaaagaag
    39601 cgatccaatt tccaaagcgg gaagaaaaaa aagagatagc agatatcaca gaacaaaaaa
    39661 cgtcgcctgc taatattgta tttttgtata aaaacaacag acgcggctca caaactatgg
    39721 gcatgacgat tttaagtcaa ttagcaaaaa aagcgaaaaa acagtttgtt atctcaaaag
    39781 aatcaataga aaaaatcaaa atccaagaag aaacgattta tatcgcacaa agagaactag
    39841 ctgtaactga acagctagaa gaaaagatcc ctcggctagt tctagttgaa cattgggtag
    39901 ctacgaatga atacgaaaaa ctcgtgaagg gagaaatata agatgtattt atcgcccaga
    39961 gaaaaacaat tattagcaga atttctcagt agcccgtcac ctgtatctat ccaaaaaatg
    40021 atgaatttat tgaaggtcag taaacggact atttaccgcg aattagataa tcttgaagtc
    40081 tcattgaaat cggtaaatgc ttctttgaaa aaagcagcta gaggctcatt tgtcattgag
    40141 gcatcagaag aagtattaga gcaattaaaa gaaaatacag atgggaaaag taatgactta
    40201 tccactgcta aacgacaaca cgcgatttta gccgagttgt tactgacgac agagccgttg
    40261 agtcttgctc atttcatgga atcatatcgc atcagtaata ccacttttta tgcagatatc
    40321 aaacagttgg aagaaagtat tcgccaactg cctttggaaa tcattcggaa ccagggatac
    40381 gagatcgaag gaccggaaaa ataccgccgg cttctgacag cgaatgtatt agaattagaa
    40441 atcaatgaat atgaactttt tcatagtatt tctttcgatg catcactgaa ttattttttt
    40501 cgattcgttg atccgcaaca tctatctctt gcacgcaaag taatcgggga agaattgatc
    40561 caaaaaaaga ccaatctaag tgatagaaag cttgaacatt tggttttgat gctgacattg
    40621 acgatggagc gtgtgacgaa aaatcattta ctgacaaatg agacatataa tagtctggca
    40681 aacaaagaat tactacagac agctaaacga ttgttttcac ggatcgcttc tgagacaaaa
    40741 caactttatc cagtaaatga gatcgtcttt tttgcaggat tattaaatga cttcacgaac
    40801 tcatttgatc agaatttctt tgaagagacg ttcgatacac agcttgctta tttagtaaaa
    40861 caactgattc aagaagtaag tgaagagacg aatgtccatt tttatgaaga tgaaactttg
    40921 tacaaaatgc tgttgactca tttatctggt gtgttttcaa gagccgtttt acaagaagag
    40981 acactgacca atccgatttt agaaaaaatc atggttcaat acgaggaagt tgcgaatgca
    41041 atccgtaaag cagtagagcg gatttttcaa gaaaaaaaat tatccgaaga agagatcgct
    41101 tatatggtcc ttcattttgc gaattcattg gaacgaagtc cgaaagaaag caatatcagt
    41161 attgcaggat tttcgccaag tggattagct tctactagca tgttggaact acgattgaaa
    41221 aggtattttc ccttcatcca acaaattcat ttctatcgga ttgctgattt aaaaaaaatc
    41281 gatttacaag aagaatatga tgttgttatt tctacatcta ttttaccagg atacggtgga
    41341 aaatatattt tagtctcccc gctactgcta gaagatgaag tcaagcagct aaaagaagct
    41401 ttttcgcata ttgagaagaa aaaaaggcaa aaaattacgt ataacagcaa taacgagtgg
    41461 atgatagaag atacttatga ggaaacaatg catttcatgg atcaaatcaa tcaattactc
    41521 ggtcgttttt ttatccaaga actggataat cctgagacga tttccgaaac gattcgcagc
    41581 ttatttcctt attttcctga aggactagta acggatgaag aggtggttca tcgccggctg
    41641 cttaagcgat atgaacaagc acctatcggt attcctcata caaatatggg gttattccat
    41701 acatccagct cattgatttc taaaccgatc ttttgtattt tcaatttgaa acatccgttg
    41761 accattgaag ggatggatag acagccagtc gatttgtccc gtatgttagt tatgctggcg
    41821 ccttcgccaa tccaagaaaa agacgggatt cttttaggaa agatcagtgg agcaatcatc
    41881 atgaatgact tgaacaccga gatcttccgc tcaggaaata aagaaatcgt ttatcagttg
    41941 ttagcgaaaa tcctgattga agaaataaag aaatgaaggt gaaaagatga tacagctacc
    42001 agcagaaaaa atattgctcg atcaatccta tcaaaattgg aaagaagcgt tgaccgagat
    42061 cggccagtgg atgacccaac aagggggaat caaaccggca tatatccaat caatgatcga
    42121 aagacaagaa aaagcgagtg tctatatcgg gaatttcgta gccttgccac atgggactga
    42181 caaagatgaa gaagtagtag aagaaggaat ctatttagtc caagtgcctg atggtgtgga
    42241 ctttggaaca aaagaggaac caaaaatcgc gacactctta tttgtagtgg ctttaaaaaa
    42301 agaacatcag ttagcggtgc tccaagattt agcctttttc tgttcagaca tcgaccaagt
    42361 aatggcgctc agtgatgcgg aaaccattga agaagtccaa atgatttttg aacatggaca
    42421 tatataacca atgttcacag taaaaaaagc tgccttgcat caagtaataa cttcaaccaa
    42481 gataaaaata agaggagcta tttttgttgg gaggttctta ttactgcgga tgcaagcttt
    42541 tgaaaactat ttattctaga aaaccgttta tttgaaatag gggacggtga caaggttttt
    42601 gtcaccgccc ctattatttt tgtgttttaa tagctctttt ttatatagat agtgcccata
    42661 cctatgaata ataaaacgat actccatata ccaagtgtca agataccatt catgaaagta
    42721 acctgttctg aagctagtgg cgagaaaata tctcctccta tgatcacatt ttgaaaatca
    42781 acataagaag ttggcaagta atgactgatt gattctatcc atccaatcga agaaaacaag
    42841 cgatactctg ctaagaaaag acaaagaagt gtacctaaaa gattgactag agaatgttca
    42901 aataaagcag atagaagaaa actgattgta cttaaaaaga gcacccacaa caacaggaac
    42961 agaaagctct ggaaaagata ctcatacatc ggcaggattc gtatctcctg accaatgatc
    43021 gttcctacag gataacggaa gtctcccaaa ccatttttga ttattactag tatagagacg
    43081 atcaaaaatg gcatcacgag acttagcata gctgcaccat aataaatggt aaaacggatg
    43141 ctatgtttct ttaccatact atccggtaat agattgacta gagagattac ctgctttctt
    43201 ttttcataag agatgatgga agaaagaaac aaggcggtta tacataaaat caaaaaacta
    43261 gaaatcattc ctgaaaaaat attttcgtag taatttgcta aagagagatg cgctggcaag
    43321 tcaaaaatag aatatctttg gatctgatgt tctttcatat aacggagcaa ctcgacacgt
    43381 ttttgttgtt caatgattgg aattccttgt ctttgaccag aaatcagtcg atccagtata
    43441 tctttttcat aatgataagt tgcttcgacc atttgctgga cattccctgt gttttttgca
    43501 tgtaagattg cttcgatcag tttgttgctt ttctctaaac tttccacaaa ttctttctca
    43561 ttctcatctt ctttcaacgt ttcaatcaaa cgttgcatct cttcatagtt atgattcagt
    43621 tcatcgaatt ctgtataagc aagtttatga tcaaaaaaaa gtgggaatgc aaagagcacg
    43681 accaacaata gaggaaataa aaagtttact ttgtttttta gtaagagatt gcgttcaaat
    43741 gtaagtaaag acatagttac acctcatata atttacgata aatagctaaa atctcttcca
    43801 tatttgagga ttctgtgacg atttctccat tttttagaaa acatgcttta gaacaaatcg
    43861 attggatatt cgcgatagcg tgggaagaaa gaagaatcgt cttcccctct ttctgccatt
    43921 gtctgatgag gctattcaag atatcaatac ttcctgggtc taatccgttc aacggttcat
    43981 cgaataacat gacagatgaa tcactgatca agtaagcagc aagcaagaca tgttgtttca
    44041 ttcctagaga catttttcca atcggcaaag taaggtactc agtcatcttc aatcgttgga
    44101 aaacagtttg gcattctact ttgctctgcc aatagtattt gatcaaagtc aaataatcaa
    44161 cagccgatag atagtcggat aactgattcg ttgattctag aaaaaagaaa gaccggcgat
    44221 aatctgtata agcagtagga gagatgttgt gaagggacaa ttgatcataa gttgttggca
    44281 gcagttgggc aatgccattc agcaaagttg ttttgccaaa tccgttcggg gcaatcaaag
    44341 cagctacttc tcctgattct actaaaagat tcaattgtcg aatcacacgt tttttaccac
    44401 gtttcatgtt catgtttttg atcagtaatg tcatagatag gcctccaata taaaatttaa
    44461 taacataata gaatggaaaa catctaaatt aaatgctaac aaaagttatt gtttgcatat
    44521 tataaaaaaa ttaaaataac agttttattt aaaattgaag ttttctgata ccgaaatctt
    44581 tttattttgc tgacatactc aaacctttgg cacaaagttt cgtgccaaag tacgactatt
    44641 tctcttttta gtaagcggat acaattagag tatcaaaagg agagagggag agacaaaatg
    44701 gaacggacga ttcccaaaaa caacatttcg ctaaaagcga gggtacaaaa attaggcagt
    44761 actttatcaa gcatggtcat gccaaatatt ggagcactga ttgcctgggg tgtattaacg
    44821 gctctattta tcccagatgg ttatttacca aatgaggctt tcgctaccat ggtaagccca
    44881 atgctaactt atctgattcc tttattgatc ggttatactg gcggaaaagt aattgctggt
    44941 gaccgcggat cagttgttgg ggcaattgca acgatgggtg tgattgtcgg aacagatatt
    45001 ccaatgatgc taggcgcgat gatcatggga cctcttggtg gatatgccat caagaaattt
    45061 gatcagctat tccaaaaacg gatcaaatcc ggttttgaaa tgctagtcaa taatttttca
    45121 gcaggtctta tcggttttgg attagcgtta ttaggttttt ctgcgattgg tccagttgtc
    45181 gatgcattga ctcaagcaat ggcaaaaggt gtagagatca ttttatctgc ccatttgatt
    45241 ccattgacaa gtatttttat tgaaccagca aaaatcttat ttttgaataa tgcaatcaat
    45301 catgggattt tgacaccatt gggaactgaa caagtagcag aggctggaaa atctattcta
    45361 ttcttgcttg aagcaaatcc tggtccagga cttggcgtat tacttgcttt tactttgttt
    45421 ggcaaaggag cagcaaaatc ttcagcacca ggagccatga tcattcactt tttaggcggg
    45481 atccatgaaa tctattttcc ttacgttatg atgaaaccgt tattattctt atcagttata
    45541 ggcggaggaa tgtcaggaag tttcattttc caattgatgg atgctggact aagagcgcca
    45601 gcttctccag ggtcgatcat tgcgattcta gcgatgacac cgttcaattc ttaccttcct
    45661 gttattttgg gcgtgattgt tgccgcagct gtttcgtttg gtatttctgc agtaattttg
    45721 aaagcggatg cgaaagatac aaatgaagac ttcgaaaaat ctgttcaaga gacaaaggca
    45781 gctaagcaag cttccaaagg tgcccaaggt attcctcaag gagctacatt ctctggcgta
    45841 caaaaaatca tttttgcttg tgatgcagga atgggatcaa gtgcgatggg cgcttcgatc
    45901 ttgagaaaaa aagtacagga agcagacttg cctcaaacgg tgaccaatca agcaatcagc
    45961 caactgacag atgactcgaa tacattgatc gttactcagg tcgaactaca agaaagagca
    46021 aaacaaaaag caccgagcgc gcgttttgtc tcagtagaaa actttttgaa ttcacctcga
    46081 tatgatgaaa tcattcaaga attaagcagc aagcagaaaa catcagtttc tgaaattgag
    46141 aatgagccag cagaagagtt tctttcagca aacatcaaac aaatcaaaga gattctactt
    46201 gtccacgatg atcgttccgg atcagcaaca atggggatga ccgtgctcaa agatatatta
    46261 gacaagaacc atttaactat cccgttaaga aaagtcaata tccaagattt aaaagatgaa
    46321 cctgattcac tgatcgtagc caaagaaagt ttagcggaaa aagtacagat ggctgcacct
    46381 caatccattc atttatcagt caacagcatg gtcaatccgc aaaaatacga atcgattgca
    46441 caatatttga aacagacagc ttaagggagg aaaacagcat gcaaagtttg ccattcgaca
    46501 tgatcgagct caatcgatct tttaaaacca aagaagaagc gatccgctat tgcggtcaga
    46561 tgcttgtaga agcagggtgc gttgatgagg aatatatcga tgccatggta gatagagaca
    46621 aaatgttatc tgtttatatg gggaatttta tcgcgattcc tcatggaaca gatgaagcaa
    46681 aaaaatatgt gaaaaaatcc ggcatctgtg tcgttcaagt accagatggt gtagattttg
    46741 gtaccgaaga agaagaaaag attgcgactg ttttgttcgg tattgcagga gtcggagatg
    46801 agcatttgca acttgtacag caaatcgcac tttattgcag tgatatggac aatgttgtcc
    46861 agctagcaga cgcgctcagc aaagaagaag taacggaaaa tctagcaatt gcttaaggag
    46921 agagaaaaat gagagcagta cattttggtg caggaaatat cggtcgagga tttattggag
    46981 aggttttggc agctaatgga tttgagatta ctttcgtaga tgtgaatcac acgatcattg
    47041 atgctttaaa tgaaagaaaa gaatacgaaa tcgaattagc agatgaatca aaagaaaaaa
    47101 tcactgtaaa aaatgtcaaa ggaatcaaca atcaaacaaa tccagaaaaa gtaatccaag
    47161 aaatcgctga agcagattta gttactaccg ccattggccc gaatatctta ccttttattg
    47221 ctgagttgat tgcaaaaggt atccaagaaa gagaaaagga aggaagcaca acatctttag
    47281 atatcattgc ctgtgaaaat atgattggcg gttcagcatt tctagaaaaa gaagtgtata
    47341 aatatcttcc ggaaacatca tttactgaca aatatatcgg ctttccaaat gcagcagtcg
    47401 atcgaatcgt cccattacaa caccatgaag atcctttatt cgtccaagtg gaacctttca
    47461 aagaatgggt cattgacgat agccaaagaa aaaataaaaa aatccaatta aaaggggttt
    47521 tatatgtaga tgatctagaa ccatatatcg aacgcaaact atttagtgtc aatactggtc
    47581 atgcgactgt cgcttataca ggtgccttgt taggttacca gaccattgat gaagcaatgc
    47641 aagatgcgtt agttgtggca caattaaaat cagttttgca ggaaacagga agtttgctga
    47701 ttgccaaatg ggggtttgat gcagagcagc atcatgcgta tattgaaaaa atcattcatc
    47761 gcttccaaaa caaaaatatc tctgatgcga tcacacgtgt cgctcggaca ccattacgta
    47821 agttaggtta tcaagaaaga tttacacgtc cagtgagaga actacaagaa cataacttga
    47881 cttgcccaca tttgacggca acaatgggga ttatttttaa ttactatgat cctgaagatg
    47941 aacaaagccg tcaattgcat gaaatgaaga tccatgaaaa cttggaacaa ttgattcaag
    48001 aagtcacagg aatcaatgat ccgaaaacaa tcgggaatat caagcaaaac gtgaaccgtt
    48061 atgccaagca agtcgcttaa aataggatac gaaaaagggg aatgtgacga aatttttgtc
    48121 acattcccct ttttcgaata actaggctgt ttgtccttca agatagggca gatccaattc
    48181 tttaccgtaa cgttgttcta aagctaggcg gatcaaacga gctgccaggt tctcatttct
    48241 tgcaaggata ggtccatgga aataagaacc gaagacattt cggtaaatga caccttcaga
    48301 ttggtcttca ccattatttc cttgaccttg taccactttc cccaaaggtc gttcaccttc
    48361 acctaagaaa gtccgaccat tgtggttttc aaatccataa taagtttcat tgaattcttc
    48421 gttatggata acgatatcac cgataaaccg gttattttcc tgacttaacg tgtagtgatc
    48481 cagtgcactg attccttgga tcttttctcc attagctccc atgtagtagt gacctagcaa
    48541 ttggtagcca ccacaaatgg ctaacatgac gccatcattt tcgatatagt tgatcaaaga
    48601 atctttcttt ttctggatgt cttttgaaat gattaattgc tcaaagtctt gaccaccgcc
    48661 aaagaaaacc aagtcatatt tatcagcatc aaaaggttca tgtatactaa cgatctccgt
    48721 atggcaagaa acccccattt tttctgctat atatttcaac atcaaaaggt ttccgttatc
    48781 accatatgtg ttcatcaaat ttccataaag gtgggcgata ttgagttcat aattagccac
    48841 ggttcattcc tcctttgata aatccttgag cagctagttc tttacgaagc tgcaatacag
    48901 cagtgtaagt agctaaaata tagccatgct cagttggcaa ttctttgatt ttttcgatta
    48961 cttctggtaa ttcttttgtt tctgtcaatt tattttctgg aatcccggca acttttagac
    49021 gtaaagtcat atctgtatga cggtctcctc cagaaataac agctgggata tccatatcgg
    49081 caaagttttc aaaattacca tcccaaatcc agctgacatc gattccgtct gcgtaattgg
    49141 catttaataa agccactaaa gaaaatggat aaggagctag accaatcata tcgattactt
    49201 gattgatacc tacaggattt ttgacaagta ctaatgtaca ttttttccca ttgatattga
    49261 tgacttcttg tcggccgaat actttctcgt catatcctaa accttgtttg attttatcag
    49321 gatcgacatt gaagtactct gccactgcag tagctgctaa agcattgtag acattataca
    49381 taccgccaac ttcgattccg tattcatgac catcgatcac gaaatctgct gacacattgt
    49441 ccatccgtac catatcagtt aatcgataat ccaattcagg acgtttgaag tcacaatttg
    49501 gacagtaata tttccctaag tttgcataag tgatcatttt ataatgcaag atgtgatgac
    49561 aatttggaca taagacaccg tctgtattgt aatgagccat ttgttcacga tcttcttcat
    49621 ggtcaaagcc ataatatttt ctaggattga ctgtttcgat agaattgaag attggtgaat
    49681 caccgttact gataatcgtt gcatttggtg actttgcggc accgtctaca atcattttat
    49741 aggtcgtata gatttctcca taccgatcca tttgatcacg gaaaatattt gtaaataaga
    49801 acagttctgg tttcatatat tctgtgactt tgctcaaact tgcttcatct atttctagga
    49861 cagcaaactt tttctgtcct ttttttggtt ttgcctgcaa gaaagtcgat acaattccct
    49921 gtaccatatt cgcaccagta gggttggtta gaacttcatc gaattgctgg cgcaaaatat
    49981 tgactgttaa agcggtagtc aatgttttac cgtttgttcc ggtgacaacg acgacttggt
    50041 agtcttttgc caaactatct agtatatgtg gatcgacttt cagcgctagt ttacctggta
    50101 aacttgatcc gcctttaaaa aaagttttta atgcccattg cgacgttttt ccaactgcaa
    50161 tcgcaaaatg actgcgtatt cccattatgt aacctccaaa aacgatttgc tgacgaataa
    50221 attcaactaa tcataccata tgttgaaaaa aaggttaaat tgcttttctt attcttaaga
    50281 aaaggttacg gctgaaagtg catgaaatcg gacaaaatcg catgatttaa caaaaaaaca
    50341 gcttgaaaca aaatgattgt ttcaagctgc tgtgatttta ttattccctc ttacctatat
    50401 tcccatttta accgataata attttctcgc ttggatattc atagcctaga tctcgtgttt
    50461 cttttggcac gaataacatc aatgtatata gcattccgat ccgcccaata aacatcagtg
    50521 tagcaatcgt gattttgccc acaacagaca agtctccggt aatccctaaa gataaaccag
    50581 ttgttccaaa cgcagaagtt acttcgatga tgatcgagat cagcgtttgt tcttctgtag
    50641 cagataaaaa aacgatacaa aagaaacaca taccaagaga aagcataaag acaacgactg
    50701 attttcgcac atcatcttga tcgattctcc gtccaaaaat attgatgtta tcttcacttt
    50761 tgaggaagga atatagatac aaaccgatga ttgctacagt agttgttcga atcccgccgc
    50821 ctacagaact aggactgcat ccgataaaca tcagtaaaga aaaaatgatc agtgttgtga
    50881 tttgaaaatc acccaaatcg tgaatctgca gaccagcatt acgagtcgta atcgaataaa
    50941 acatcgaatt gatccatttg acactcatat tggaatcttg gaataaatgg tccttttcca
    51001 atagataaat caagacagtt cccgaaacaa acaagataac aaaagctaac acagctaatt
    51061 tggtaaacaa ggaaaaacga aacggaagct tcgcattgga acgtttgtat aatagccatt
    51121 cccggcactc catcaataca ggaaaaccaa ttcctccgac aaaaatcaaa aacatgatca
    51181 ggatcaagaa aaaatagtca tgtgcaaagg gcttgatgga atccccagtg acgtcaaacc
    51241 cagagttcgt gacagccgat atcgcctgat aaaaaccgta aaaaacggca tcacgccaac
    51301 gatcaaaata gcctctgtaa taaaaataaa tagaaaagaa tgtcccaaaa agtaattgaa
    51361 accagattaa aatagcaaaa gtgatccgaa tcagtcggac gataccagac aaacgcggtt
    51421 gattcatatc cgtcatgatc agctgtcgct gtttcagtgt gatccgtcgt ttagaaaaaa
    51481 tgacaaaagc agtagaaatc atcatgatcc ccaaaccacc aacttgaaat aatatttcca
    51541 gcagtataat accgttatca ttgaacacag aattgatatc aaaagtcgat agtccagtca
    51601 cactcactgt actgattgcc atgaacagca tgtccacaaa agaaacatgg ctatctggtt
    51661 cgcgaaaaat cggcatataa aatagaacga gtgataagac cgtcatcaat atataataaa
    51721 aaacgatgat ttggatcgat gagaaatgat tggcagcaaa cttctgtcct cgccgctgca
    51781 gccttcgaaa gaataaatca acatgcacag tactatcctc ctgacaatag tcgtttctac
    51841 cagtatacaa ggggaattag gaatactcaa agaaattatc aaacagataa caatacagct
    51901 ttcgattaaa atagaaatgg cttataataa ggaaaatcct agaaaaaaga aggaggagga
    51961 ataacaaatt gattaatgct caacaaatcg tagaaaaaat gaaaaatcaa aaaataaatt
    52021 atgatacagt tctgaaaaaa atgatcatgc aatgggagaa agaagaagta aagcctaaga
    52081 tattaataca tacatgctgt gctccttgca gtacgcacac attggaattc atgtctcaat
    52141 atgcagagat cacgttattt ttttcaaatt cgaatattca tcctaaaagt gagtatttgc
    52201 gaagattaca tgaacaaaaa cgatttgtag accaatttaa tgagaaaact gaccatcatg
    52261 ttgcgctaat cgttgatgaa taccgtccaa atgaattcac caaaatggtt ttaacaaacc
    52321 atcttgaaaa cgaaaaagaa ggtggtgctc gctgcagtgc atgcttcaat atgcgattag
    52381 atcttgttgc gcaaaaagcg caagaactag gatacgatta ctttggaagt gccctgacca
    52441 tctcgccaaa gaaaaatgca gctttgatca atcagatcgg aatggatatc caaaagattt
    52501 acgggacaca atacttgcca agtgatttca agaaaaacaa aggctatgaa cgctctttgc
    52561 aaatgtgcaa agagtatgat atttatcgcc aatgttattg tggttgcgtg ttcgctgcca
    52621 agcagcaagg atgcgatctg aaagaaatca atcgggaagc aaaagcttac ttaaaaacat
    52681 caaccgtagt ttttgaggaa aattaaagaa atctttcgga aaaacgtaag aaaaaaataa
    52741 agaatttatg gtagaaataa ggtagactgt ataatttgtc cgtagtaaag taaggaaaca
    52801 agaataaatt tcaatgaagg aagagataag atgcgaaaag tagcacctat tttgaagcca
    52861 atcgctgtat taacaatctc gacgattgtc gcgactacta caggtattgt gaatagttcc
    52921 agctaccgtg caccaagttc aagttctagt actgtagaag aaacaacacc aactacttca
    52981 gtctcgacat ctgaaccaac aacaacggaa agtttgaatg aaaccactac gacaagtaca
    53041 tgggacgata caacaactag ttcgtcgtat gatgagacat atacaactga cagctcaagc
    53101 gaaacaagta atacggtacc agatggcact ccatcggatt caaccaatcc aagtacaagt
    53161 ccggatacaa gtgctagctc cgaaacgata ccaagcagta cgactgtaga tgataacacg
    53221 caaaatgatg atgcagcacg tacagcacag caaaaccaag attcaggaaa tacccaaacg
    53281 tataaccagc aagcaggtga ttctcaatga catttttact agaccttatc aacggggtgc
    53341 ataaattcct tggctattta gacatcagtc ctaaatatct taaccgtgga tatacgattt
    53401 taagtgtgat tcccacgctt tatttattga gaattgttta tggactttgg caaaaccaaa
    53461 actatcttca atttttctta tatggacttg catttctagt gcttgtttat tttactgttt
    53521 taaatgtatt ttattacttt ttagataaaa acacgaaagc cgatgtcact cagctttttg
    53581 tgaagtattt acctgatgaa gcgttcaaca tccagacaga agaatcgata gataatggaa
    53641 attcgatcga taaagtgaat acacaagagg tcatggtttc ttatgacgaa gattatcaat
    53701 tgaaattagc cgagaatatg cggtatttga ttggaaatgg tgagatcaaa acaaatgaac
    53761 tcggtcgcat ggatggattt ctggtcgata gaaatacatt gtatccttat tactatgtca
    53821 agaaaacagg agagaaagag tatgttctaa atattggacg aagctactcg gacatggaaa
    53881 aagtagggac cattcgactt gactcaacag aagacacatt ggaacctgtc ggattgttca
    53941 tcgtcggcgg tgattttgtc aaagacgggt tacgctatca tgagccttat cgtctgaaat
    54001 tgattgcaaa aaaggcacag tcgcaggaag aaccagtggt ttatagtcgt agccaaagaa
    54061 gaagacattc ataatgtgat gaaacgccgt gtatcagtgg ttgcgataaa aacgtttatt
    54121 cggaataagg gggatgtgac acgtcttttg tcacgttcct tttttatata acgagaaaga
    54181 taagacgaac acatcactta ttctttcctt tcatttataa acattgtaga ataaagaaag
    54241 tacaacgaag tattgtgaaa aaacgtaata aataacgagg aaaatacaag taggaggtaa
    54301 gctgtgaaag aatttttgaa aaattggggg attttgatcc ttgtattagc ggctatttta
    54361 ttagctagag tgtacgtgtt tacaccagtt actgtaaacg ggcattcgat ggaccctaca
    54421 ttaagtgatg gacaacgatt gatttcatct aagatctcaa actatgaacg aatggatatt
    54481 attactacaa aggaaccggg ggatgaagaa cggatgattg ttaaacggat catcggtatg
    54541 cctggagata cagtcaagat ggaaaatgac cagttgacca ttaatggcaa aaagtatgat
    54601 gaaccttatc tggatgagtt caaaaaagaa ttttcagaag ataagttaca aggagaatat
    54661 gcttatagct caggattcca agcacaagca gaaagttcat ccacctttac gagtgacttt
    54721 gaatatactg tgccaaaagg aaagtatctt gttctaggcg ataaccgctt gatttcaaaa
    54781 gacagccgaa tgtttgggtt agtcgacaaa gatatgatcc aaggaaaagt tgttttccgt
    54841 tattggcctc tttcagaaat taaaatcatg tgatgaaaaa ataaaaagaa gctgtgacat
    54901 gttttttgtc acagcttctt tttccgaaaa ttccttctta tttcttggag ttgctcactt
    54961 tttttgcaat ctcattttaa gctttttggc tgatggataa gaaaagcgaa gcgtttttgt
    55021 tcataaatag cttttcccca cgatggaagg tatgactagt agcagagaca aagcccgttt
    55081 tttccaatcg attattcaat tcttcttggc taaagccatt atggactttc ggatgactaa
    55141 tttgttcatt cttgtcaaag tctacgatga tcaattgacc gccaggagct aaaatttcat
    55201 aaagttttgt aagaatattc tccgtatcag gaatgtgtaa aagtacaagt gatagaagga
    55261 tgagattagc ttggacagca ggaaattcga cagaagcatc agcgtggatc gttcggacat
    55321 ttttcagatc tgctgcttgg atcttttctt ctgccatttt cagcatagtt tctgaagcat
    55381 cggcaatgat cagttctttg aatctttccg caagcggtaa actaacaagc ccagttccgc
    55441 ctccgtaatc cagcaggact ttttggtggc tgtcgcgcgg tagataagta gttagttctt
    55501 cgcgaataat cttggctaat gcttgacggt caggggaatc ataatggtta gcgatggaat
    55561 caaaaatatt ttttgtcatc aaatagctcc ttacgtaaaa ctttgaaatt ttaacttatt
    55621 atagcacgtg aaaagaaaga aagcatgcta tactcaattc aaaaagggag aatctagaaa
    55681 gaatatgccg atatcgtttg taaaagatag agaggaaaaa ggaaagtgtg tccgagaaat
    55741 attactagac ttgcctgagt ggtttgggct tcctgaatca acggaaaaat atatcgagga
    55801 atcaagtaaa ctacctttat ggtgtgaaaa aagaaaagaa gaatatttag gctttatcac
    55861 tctttcacag acaagtgaag atactgccga aatctattct attgtatggg aataaaaaaa
    55921 gactatcatc accaaggatt aggaacaaaa ctttttaaag aagcagaagg ttacgcagca
    55981 aaacattaca aatatttgca agtgaagact gtagacgagg ggcactactc tatatatgat
    56041 caaactattt gtttctacga atcattagga ttttcaaggt tggaagtttt tccaaattta
    56101 tgggatgaat ggaacccatg tttagttctt gtcaaaaaat tggaacaaaa gtgagcaata
    56161 gatttaatac taatctgtgc ttgatgaaaa ttctcactgt aaaaaatgct gaaaatcaag
    56221 tataatacca taggaacaaa ctggaaggtg aaaaagagca tggcacaact attttttaag
    56281 tatggcgcaa tgaatagcgg caagacgatc gagatcctaa aagtcgcaca taattatgaa
    56341 gaacaaaata agccagttgt cttaatgaca agcggtattg atacaagaga tggtttaggc
    56401 gttgtttcaa gccgaatcgg actgaaacgt aatgcgatgc cgatatttga agaaacagac
    56461 gtatttgacg taattcagaa aatggaaagc aaaccctatt gtgtgctgat cgatgaagcg
    56521 caattcttaa aaaagaaaca tgtcattgct tttacgagaa ttgtggacga attggatatt
    56581 ccggttatgg catttggttt gaaaaatgat tttcgaaacg agttatttga aggatcaaaa
    56641 tatttattgc tttatgcaga caaaatcgaa gaaatgaaaa caatctgttg gttctgccac
    56701 aaaaaagcaa tcatgaattt acattatatt gatggaaacc ctgtttatga aggcgatcaa
    56761 gtgcaaattg gcggaaatga agcttattac cccgtttgtc gaaaacatta ctttcaccca
    56821 tcaatgatta cagaaggaga ataagcatgt acgatcaatt acaagcaatt gaagaccgtt
    56881 atgaagagct tggagaactt ttaagtgacc cagaagtgat ttctgataca aagcgtttta
    56941 tgcagctttc aaaagaagaa gcaaacacgc gcgaaacagt cgaagtttat cgtcgttata
    57001 aagaagtagt acaaggaatc accgatacag aagaactgct tggtgaaaaa ctggatgatg
    57061 aaatggctga actagcaaaa gaagaattat ctgaattaaa aaaagaaaaa gaagtcttag
    57121 aagaaaaaat caagatcttg cttttaccaa aagatccaaa tgacgataaa aatatcatca
    57181 tggaaatccg tggagcagca ggcggagacg aagcagcatt attcgctggc gatcttttca
    57241 atatgtacca aaagtatgct gaagcacaag gctggaaaac agaagtgatg gaagctaacg
    57301 ttaccggaat cggtggatat aaagaagtga tcatgatgat caccggagac aacgtatatt
    57361 cgaaattaaa atatgaaagt ggtgcgcacc gcgtccagcg tgttccttca actgaatcgc
    57421 aagggcgtat ccatacttcc accgcaacag ttgtcgtgat gccagaagct gaagaagtag
    57481 agatcgatat tgaagacaaa gatatccgta cagatatcta tcatgcttca ggtgctggcg
    57541 gacagcacgt caataaaaca gcatctgctg tacgtttgac tcacttacca acagggatcg
    57601 ttgttgctat gcaagatgaa cgttctcaaa tcaagaaccg tgaaaaagca atgaaaatct
    57661 tgcgtgctcg tgtgtacgat aagatccagc aagaagcaca aagcgagtat gatgccaatc
    57721 gaaaatcagc agtcggaact ggggatcgtt cagaacggat ccggacgtat aatttcccgc
    57781 aaaatcgtgt gactgatcat cgtatcggat taactatcca aaaattagat cagattttag
    57841 cagggaaatt agatgagatc gttgatgcat tgatcatgta tgatcaaaca tcaaaactgg
    57901 aagctatgca aaatgggtaa aatgacctat cgagaagtcc tgtcacgggc ttcttctttt
    57961 ttagaggatc atgggaaaga agcatatagt atccaatttt tatttttgga acgaaaacaa
    58021 tggaaaaaac ttgattggct ccttcatatg aacgaagaga tttcagaaga agaacagcgg
    58081 ttgatcgaaa ccgatctgcg acttctgctt gcagatcatc cacctcagta tttgctagga
    58141 tatgcagatt tttatgatca tcggttaaag gtaactgaag caacactgat ccctcgacca
    58201 gaaacagagg agttagtcga atggtgttta gacgagacgc ctggtgtacc gttagaagtg
    58261 atagatattg ggacagggac tggagcaatt gctattagtt tgaaagcagc aagaaaaaat
    58321 tggcatatat ctgctgtgga tctttcagaa gaagcattag aagtagcgaa agaaaatgct
    58381 cagaaagaag gaacgaaaat cagtttttat catggagata cattaggacc agtaatggat
    58441 cagcaatttg atgtgattat ttctaatccg ccgtacatca gtcggaatga atgggaactg
    58501 atggatgaaa gtgtccggtc attcgaacct aaaatggccc tttttgctga gaacgatgga
    58561 ttagctattt atgaaaaaat agcagttgaa gcaccaagcg tactaaaatc aaatggaaag
    58621 atttttttag aaatcggatt tcgccaaggt gaagcagtaa aaaaaatatt tcagcaagca
    58681 tttccagata aaaaagtggc gataaaaaag gatctttttg gcaatgaacg aatgatccaa
    58741 gtttatggtt gaagttgtgg atgaaaaatt ttttatttgt ttttttatta taaaaaaaca
    58801 cttatttgac aaggatgtaa acgacttttt ggtttatcaa caagtttatc cactattgac
    58861 aaatagatta tccacaaaca gtggaaaact tgaggataaa ctcagacaaa ataagaatgt
    58921 atgaaaaagg ggttgtggat aaactgtgga aacgaaacga tatgatgaaa cagaattgca
    58981 agaggcagcc gaagcactga aagaaggaga acttgttgct tttcctaccg aaacagtata
    59041 cggattgggc gccaacgctc ttttaccaaa cgctgtcaag aaagtgtttt ctgtaaaagg
    59101 gagaccgcaa gataatccat taatcgtcca tgtcgcctct tttgaacaag taaaagagta
    59161 tgtggacaac tttcatccgg cagcagagaa gattgtgaag aacttctggc ctggaccatt
    59221 aactttgata tttaagatca aaaaagacac tttgccctca gttgtgacgg gaggattatc
    59281 cacagctgca tttcgtatac cggataacaa aaaaacacta gaagtaatcg aactttccgg
    59341 tgttccgctt gtcggtccaa gtgcaaatac gtcgggaaaa ccaagtccaa cgactgcaga
    59401 tcatgtatat catgatctac aaggaaaaat taccgggatt attgatgatg gagctacgcg
    59461 aattggtgta gaatccactg ttcttgattt aagtgatcca acagccatgc ctatgatttt
    59521 acgaccaggt gcagtaacga aagaacaaat cgaagcagtc atcgaatctc ctgttgctat
    59581 tgatcagcat cttgtaaaag aaaatgaaac accaaaagcc cctgggatga aatacaaaca
    59641 ctactcaccc gatacacgtg ttctgatggt cagagatggc gattggtcaa ctgcagttca
    59701 atgggcaaaa aataagaaga tccgtgcagg tgtcatagca agccccgaaa ttgcggatca
    59761 agtgcggaca gatactgcag ctgtttatat gtataacgac aactcagtag aagcggcggc
    59821 taaaggattg tttgcaggtc taagaggatt agatgaacca acacttggat tagatttgat
    59881 ttttgtacaa gtgtatccag aaactggatt ggggaatgct tacatgaatc gcttgaaaaa
    59941 agcagcagga caaaattatt ttgaaaaatg atcaattggt tattgtcgca ttcttttagt
    60001 atgatacaat gctatcaagt aaatcttcta tcagtgaacg aaaaactaag cgtaactaat
    60061 cgtaatagaa gagatacgaa tatttggcaa tttgtttttg atgggaaaaa taaaattgta
    60121 ggaaaataaa aaggagctgt tgtttgtggt agattacaaa acgtttgacc ctgatctttg
    60181 ggctgcgatc gccaaagaag aagaaagaca agaacataat ttggagctga ttgcctctga
    60241 aaactttgtc tccgaagcag tgatggctgc acaaggaagt atcttgacaa ataaatacgc
    60301 agaaggttat ccagggcatc gctattatgg tgggtgtgaa tttgtcgata tcgttgaaaa
    60361 tttagcgatt gaccgtgcaa aagaattatt cggagcgaaa tttgcgaacg tccagcctca
    60421 ctccggctct caagccaaca cggctgctta tctcgctttg gtagaaccag gggacacgat
    60481 cttaggaatg gatctttccg caggcggaca tctaacacat ggatcaccag tcaacttcag
    60541 tggcaaaacc tatcactttg ttgcttacgg tgttgatccg acaacagaag ttattgatta
    60601 taatgtggta cgtattttag ctagaaaaca tcaaccaaaa ttgatcgtag caggtgccag
    60661 tgcttacgga cgaacgatcg actttgcgaa attcagagaa atcgcagatg aagtaggcgc
    60721 aaaactgatg gtagatatgg cacatatcgc aggattagta gcagcaggac ttcatcctaa
    60781 tccagttcct tatgcagata tcacaacgac taccactcat aaaacattgc gtggccctcg
    60841 aggcggcatg attttgacaa acgatgaagc attagcaaaa aaaataaata gtgcggtatt
    60901 tcctggtatt caaggcggcc ctctagagca tgtgattgcg ggtaaagcag tagcattcaa
    60961 agaagcatta gatcctgcat ttaaagaata tagtgaacaa atcatcgcca atgcaaaagc
    61021 aatggtcaaa gttttcaatc aagcaattgg aaccagagtc atttcagggg cgacagataa
    61081 tcatctgatg ttgatcgatg ttcgcgagtt aggtataaat ggaaaagaag cagagagcat
    61141 tttggacagt gtgaatatca ctgtaaataa aaactcgatt ccttttgaaa cactaagtcc
    61201 attcaaaaca agcggtattc gaatcggcac gccagcaatc actactcgcg ggttcaaaga
    61261 agaagatgct gtaaaagtgg ctgaactagt tgtcaaagca ttacaagcaa aagatgataa
    61321 cgctcaacta gacgaagtca aaacaggtgt acgcgaatta actgaaaaat ttccgttgca
    61381 taaaaaataa aaatttaaat tccattagta aacagaaaag ttcaatgttg ggcttttctg
    61441 tttatttttt gggaaaacgg attctttgac aatatcggtg atgctctcta gtatatgagg
    61501 tgtaatttta gtcttcacac taaatctcag ggaggaaaag tcatgactga tcgtatctcg
    61561 ttagaaaagg aagcagtagc attcagtgat gcaaatgcgc ctcatccgcg tatctatgaa
    61621 ctagcaccgg aagaaggaag agttttacta gaagaagtac aagaatcacc tgtgaacaaa
    61681 tattccgtcg atattgaaga cttgatggtg gatactggcg aatggggaaa actcaatatt
    61741 cgtctcgtac gaccagaagg gaatatggat aaattaccag ttatttttta tattcatgga
    61801 gcaggctggg tttttggcag tgctcaaacc catgataaat tgatcagaga acttgcggtt
    61861 cgtacgaatt ccgtcgttgt ctttccagaa tatagtcgat caccagaagc aaaatatcca
    61921 acagcaattg aacaaaatta tgctgtgttg caacagctga ataaattagc caagtcaaaa
    61981 aatctggatc tcgagaatct aatcgttgca ggagattcag taggtggtaa catggctaca
    62041 gtcatgacga tcttaacaaa agaacgaaag ggcctcccaa tatccaagca attgctgtat
    62101 tatccagtca caaacgctga ttttgataca gattcctatc atgaattcgg agaaaactat
    62161 tttttgacaa gagaaggaat gatgtggttt tgggaccaat atacgacgaa tgaagaagag
    62221 cgtgctgaaa tcacagcttc gcctttgaga gcaaatataa atgaactaaa tgatcttcct
    62281 tcagcaatga ttttaacggg agaagcggat gtattaagag atgaaggtga ggcttatgcg
    62341 cgcaaacttc gtgatgcagg ggttcctgtt acacaagtac gcttccaagg aatcatccat
    62401 gattttgtca tggtaaatgc tatggatcag acaaaggcaa cgcgtgcagc aatggcatta
    62461 tctacttctt ggatcaagaa aaaagaataa gctttgcttg tccgcaggaa aacttctttt
    62521 caaaaacgga ttttcttagg aaaaatggcg agtttgacta gtcaatcact tgattttgtt
    62581 ttacaattaa aaagaaacga cagaacaaag gagagcttaa aaaatgggca aatttcaagt
    62641 gatcgatcat ccattgattc aacacaaact aactattatt cgggacaaaa actgcggaac
    62701 aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg atggcatatg aagtatcacg
    62761 ggatatgcca ttggaagaca tcgtgatcga aacaccgatc actgaaacaa cacaaaaaac
    62821 gttgtctggt aaaaaagtag cgattattcc aatcttgcgc gcaggtattg gaatggttga
    62881 cggtatcttg gaattgattc cagctgctaa agtgggacat atcggtcttt atcgagatga
    62941 agaaacattg gaaccacatg aatatttcgt caaacttcca gaagacattg atgcacgtca
    63001 gttgtttgta gtagatccaa tgttggcaac aggcggatca gcaatcatgg caatcgatgc
    63061 attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt ttggtagcag cacctgaggg
    63121 agtaaaagct ttgcaagaag cgcatcctga tattgacatc tatacagctt ctctagatga
    63181 aaaattgaat gaacatggtt atatcgttcc tggactaggg gatgcgggtg accgtttgtt
    63241 tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa cttttgtcac atcctttttt
    63301 ggtcattttt aacgtttgat gcagaacagc tttttcacag ctttcgtttg catataaaaa
    63361 aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt ttttagttct ttgtagttaa
    63421 cgattactac acccttactt tgcgtcatca agttcgcatt ttctgcagtt tcatcaatct
    63481 caacaacagc ggaatttttc aattgctttg aaatcgttcc agtttgcgac ttgccgtgaa
    63541 ttgtgaacat tacgtgagta ttgatcttga atttttcacc aatttccgat ggtacgatag
    63601 ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc tgcctttcac atcaattata
    63661 ccacgtttta tgaaaaatat catgaaatta aactaatttt ccatcggact ctttacaatc
    63721 agccaaatag agtatataat ttcttttgga taaggccagc ttagctcagt tggtagagca
    63781 acgcactcgt aacgcgtagg tcgtaggttc gatccctata gctggcatta aaagagaaga
    63841 cagatatatc aaggattttt aatccattga tacatctgtc tttttgttta aaggaaataa
    63901 agtatgcttt cttagcaata gagcatgtat tatttctata aaaatttagg agaataaaac
    63961 gactagcaat atagctgtga aatcaaatat cacatgaact gcccaacttg taatgatcga
    64021 gttgctttta atggcagctt ggttgaataa caatcttgcc accccttgta tcaaaaggat
    64081 gtgtgctaca gtgtgtaaaa tatttccatt atcataagta ctgtaatgga taaatccaaa
    64141 gatgattgct gacaagaaac tagcgacagg aatgctgaac ttactagaaa aaatagcgag
    64201 aaaatacact gagaacaatt cttctccaag tatcatgata ggcaaaataa taaataataa
    64261 cgggctgaaa gcctcattta ttggattgcc tgttaaaccc catttcaaaa tttgttgcaa
    64321 aaataccgag actgcggcac tgattactac attgataaaa aaatatttgg ctatattttt
    64381 gacaggagcg acaggtttcg agaacatttt tttcgtatct tttttaccaa aaacaagtgt
    64441 aaatgctata aatccagtga tagttaaaga aaaaatgcta ataatggatg gcgcaaatag
    64501 caaccctaac aaaccgagta caacgacaat agtgcccaat atcccttttt taagttctat
    64561 tttttttcct aaaatgttat tcatgataat aactcctact attcagtttt tgaagataga
    64621 gtcagtatac atgaagagtt aaaataggca atgttttttt atggatattt gtattgctaa
    64681 aaatttatct tatatattca ttttgattat actactttta tttgtaaaaa ttttctagca
    64741 aaaccaagtg cttgcaactg attaagcgta caatttttta gatgttccga gcgtacttca
    64801 atcatttcaa aggagttgct acttaaatcg atatttttta aatcagtatc aaaaaagcta
    64861 gcattttcta aatcattagt agaaaacatt attttagtga gggaacagtc tacaaaatct
    64921 gcgcttgata agacagagtg ctcaaaggta ctttctttta tgtttgaacc agataaattt
    64981 acatagtcca ttttacaatt ctcgaataac gtgttcccaa tataactttc catcaattta
    65041 gtacctgtta atttacaatt gacaaatttc acgcgatgca acatgctttt tgtcaatata
    65101 cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa tctccattct ttgaaaatca
    65161 ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa taattggatg gcagattatt
    65221 tggttttcga tgacctcttt ttgtgaaagg atattataca gttcaaaatc ttcgttttca
    65281 tagatatgaa aaaaggatcc tttattcaag cttgcttcat taactttagg aaaaatcata
    65341 tttaattacc tccattttca taaagtcata ttaacaaaag ctaaaccaac ttacattagc
    65401 agaaaatgag ataggttttc gcctttttcg ataacgatag tgtcgctatc tcttgaaacg
    65461 ttcttagaat tgccctgaga gacacaaatt aggcgtaatt ctaataaaaa tcttagaata
    65521 aaaattttta ctgatttaat ttagaatgtt tttaaaataa aaatcgttgc cataacagtg
    65581 tattgaaagg ttttaacaat ccagtaatga tttaaaaaaa tctagatgta gcgtatactt
    65641 tgtttataga atgtagtggg aagaaatata tttttgggag gaaatcgtga tgtctaaaag
    65701 tgaagaaata gaaaacatgg tactaaaatt atatgatctc gttttaaatc cagagatcaa
    65761 agaaaaggaa cgtatacttt tgatagacgc aaagacaggc ttagaaaaag gtcagtatta
    65821 tcctaaagtc ataaataatt tggaacgtag tttacgtccg ttagcaatca ggggagaatt
    65881 gtctaaacca gtatcaccgt tttatatgga gatatcaaca ataggaaaat ttgaaaaaga
    65941 attaggtcga ggaatggctt cggcaccgat tacttttggg catttataaa aacgagttat
    66001 tcatctcaat gaattaaaaa gtatagtcaa tctcataatg caggctaggt tcatttattt
    66061 gaacagagtc tgtatttttt tattctaaat aaagaaacag atgtttcttt atattgtatt
    66121 ttatccttta attaacacaa aaacagctag tataaactaa taatgttaat taaaagtgta
    66181 ttttatgaaa ctatcgttat ttattaatgt tgcatttttg taagttaaaa attggaaata
    66241 tagattgaaa aaatgaaagc aataagtaaa ataaaatgaa tatatgcgaa aaaataattt
    66301 acattaggaa aaaccttgta aaaacagttt tttggcatat ttatttttta taaatatttg
    66361 taaaataact atcaattttt tagttaaaag attggaaatg agtgggggag gaattgaaga
    66421 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
    66481 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
    66541 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
    66601 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
    66661 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
    66721 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
    66781 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
    66841 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
    66901 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
    66961 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
    67021 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
    67081 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
    67141 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
    67201 gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact
    67261 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
    67321 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
    67381 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
    67441 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
    67501 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
    67561 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
    67621 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
    67681 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
    67741 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
    67801 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
    67861 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact
    67921 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
    67981 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
    68041 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
    68101 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
    68161 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
    68221 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
    68281 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
    68341 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
    68401 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
    68461 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
    68521 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
    68581 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
    68641 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat
    68701 tccgatttat aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa
    68761 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
    68821 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
    68881 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
    68941 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
    69001 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
    69061 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
    69121 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
    69181 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
    69241 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
    69301 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
    69361 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
    69421 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
    69481 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
    69541 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
    69601 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
    69661 ccaaataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
    69721 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
    69781 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
    69841 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
    69901 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
    69961 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
    70021 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
    70081 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
    70141 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
    70201 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
    70261 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
    70321 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
    70381 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
    70441 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat gaaacaagtt
    70501 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
    70561 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
    70621 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
    70681 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
    70741 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
    70801 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
    70861 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
    70921 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
    70981 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
    71041 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
    71101 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
    71161 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
    71221 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
    71281 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
    71341 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
    71401 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
    71461 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
    71521 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
    71581 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
    71641 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
    71701 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
    71761 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
    71821 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
    71881 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
    71941 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
    72001 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
    72061 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
    72121 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
    72181 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
    72241 acctccttta cataatttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
    72301 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
    72361 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
    72421 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
    72481 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
    72541 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
    72601 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
    72661 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
    72721 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
    72781 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
    72841 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
    72901 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
    72961 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
    73021 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
    73081 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
    73141 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
    73201 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
    73261 cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag
    73321 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt
    73381 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
    73441 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
    73501 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
    73561 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
    73621 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
    73681 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
    73741 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
    73801 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
    73861 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
    73921 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
    73981 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
    74041 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
    74101 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
    74161 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
    74221 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
    74281 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
    74341 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
    74401 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
    74461 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
    74521 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
    74581 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
    74641 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata
    74701 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
    74761 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
    74821 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
    74881 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
    74941 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
    75001 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
    75061 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
    75121 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
    75181 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
    75241 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
    75301 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
    75361 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
    75421 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
    75481 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
    75541 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
    75601 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
    75661 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
    75721 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
    75781 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
    75841 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
    75901 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
    75961 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
    76021 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
    76081 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
    76141 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
    76201 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
    76261 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
    76321 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
    76381 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
    76441 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
    76501 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
    76561 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
    76621 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
    76681 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
    76741 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
    76801 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
    76861 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
    76921 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
    76981 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
    77041 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
    77101 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
    77161 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
    77221 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
    77281 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
    77341 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
    77401 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
    77461 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
    77521 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
    77581 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
    77641 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
    77701 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
    77761 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
    77821 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
    77881 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
    77941 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
    78001 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
    78061 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
    78121 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
    78181 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
    78241 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
    78301 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
    78361 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
    78421 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
    78481 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
    78541 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
    78601 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
    78661 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
    78721 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
    78781 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
    78841 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
    78901 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
    78961 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
    79021 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
    79081 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
    79141 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
    79201 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
    79261 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
    79321 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
    79381 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
    79441 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
    79501 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
    79561 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
    79621 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
    79681 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
    79741 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
    79801 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
    79861 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
    79921 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
    79981 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
    80041 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
    80101 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
    80161 acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg
    80221 tgtac
//
LOCUS       DACGLH010000006        77984 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.007, whole genome shotgun sequence.
ACCESSION   DACGLH010000006 DACGLH010000000
VERSION     DACGLH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.007"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..171
                     /locus_tag="IXT21_000408"
     CDS             <1..171
                     /locus_tag="IXT21_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4867842.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            346..642
                     /locus_tag="IXT21_000409"
     CDS             346..642
                     /locus_tag="IXT21_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867843.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="IXT21_000410"
     CDS             649..2601
                     /locus_tag="IXT21_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ4867844.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IXT21_000411"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IXT21_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ4867845.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3532..4572
                     /locus_tag="IXT21_000412"
     CDS             3532..4572
                     /locus_tag="IXT21_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4867846.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4670..4858
                     /locus_tag="IXT21_000413"
     CDS             4670..4858
                     /locus_tag="IXT21_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4867847.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4916..5269)
                     /locus_tag="IXT21_000414"
     CDS             complement(4916..5269)
                     /locus_tag="IXT21_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4867848.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5800..6222
                     /locus_tag="IXT21_000415"
     CDS             5800..6222
                     /locus_tag="IXT21_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4867849.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6219..6449
                     /locus_tag="IXT21_000416"
     CDS             6219..6449
                     /locus_tag="IXT21_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4867850.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6947..7147
                     /locus_tag="IXT21_000417"
     CDS             6947..7147
                     /locus_tag="IXT21_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ4867851.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7174..8367
                     /locus_tag="IXT21_000418"
     CDS             7174..8367
                     /locus_tag="IXT21_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4867852.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="IXT21_000419"
     CDS             complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="IXT21_000419"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ4867853.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10059..10982)
                     /locus_tag="IXT21_000420"
     CDS             complement(10059..10982)
                     /locus_tag="IXT21_000420"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ4867854.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11178..11783
                     /locus_tag="IXT21_000421"
     CDS             11178..11783
                     /locus_tag="IXT21_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4867855.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12091..13107
                     /locus_tag="IXT21_000422"
     CDS             12091..13107
                     /locus_tag="IXT21_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4867856.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13248..14549
                     /locus_tag="IXT21_000423"
     CDS             13248..14549
                     /locus_tag="IXT21_000423"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ4867857.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14595..15257
                     /gene="deoC"
                     /locus_tag="IXT21_000424"
     CDS             14595..15257
                     /gene="deoC"
                     /locus_tag="IXT21_000424"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ4867858.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15278..15676
                     /locus_tag="IXT21_000425"
     CDS             15278..15676
                     /locus_tag="IXT21_000425"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ4867859.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15742..16824
                     /locus_tag="IXT21_000426"
     CDS             15742..16824
                     /locus_tag="IXT21_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4867860.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17036..18598
                     /locus_tag="IXT21_000427"
     CDS             17036..18598
                     /locus_tag="IXT21_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867861.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18591..19724
                     /locus_tag="IXT21_000428"
     CDS             18591..19724
                     /locus_tag="IXT21_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4867862.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19724..20680
                     /locus_tag="IXT21_000429"
     CDS             19724..20680
                     /locus_tag="IXT21_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4867863.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20779..21945
                     /gene="deoB"
                     /locus_tag="IXT21_000430"
     CDS             20779..21945
                     /gene="deoB"
                     /locus_tag="IXT21_000430"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ4867864.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21969..22787
                     /locus_tag="IXT21_000431"
     CDS             21969..22787
                     /locus_tag="IXT21_000431"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4867865.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22804..23511
                     /gene="deoD"
                     /locus_tag="IXT21_000432"
     CDS             22804..23511
                     /gene="deoD"
                     /locus_tag="IXT21_000432"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4867866.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23809..24495)
                     /gene="gpmA"
                     /locus_tag="IXT21_000433"
     CDS             complement(23809..24495)
                     /gene="gpmA"
                     /locus_tag="IXT21_000433"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ4867867.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24667..25248)
                     /locus_tag="IXT21_000434"
     CDS             complement(24667..25248)
                     /locus_tag="IXT21_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4867868.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25381..26064)
                     /gene="rpiA"
                     /locus_tag="IXT21_000435"
     CDS             complement(25381..26064)
                     /gene="rpiA"
                     /locus_tag="IXT21_000435"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ4867869.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26383..26796
                     /gene="rpsL"
                     /locus_tag="IXT21_000436"
     CDS             26383..26796
                     /gene="rpsL"
                     /locus_tag="IXT21_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ4867870.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26880..27350
                     /gene="rpsG"
                     /locus_tag="IXT21_000437"
     CDS             26880..27350
                     /gene="rpsG"
                     /locus_tag="IXT21_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ4867871.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27444..29531
                     /gene="fusA"
                     /locus_tag="IXT21_000438"
     CDS             27444..29531
                     /gene="fusA"
                     /locus_tag="IXT21_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4867872.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29703..30890
                     /gene="tuf"
                     /locus_tag="IXT21_000439"
     CDS             29703..30890
                     /gene="tuf"
                     /locus_tag="IXT21_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ4867873.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31297..31605
                     /gene="rpsJ"
                     /locus_tag="IXT21_000440"
     CDS             31297..31605
                     /gene="rpsJ"
                     /locus_tag="IXT21_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ4867874.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31636..32265
                     /gene="rplC"
                     /locus_tag="IXT21_000441"
     CDS             31636..32265
                     /gene="rplC"
                     /locus_tag="IXT21_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ4867875.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32293..32916
                     /gene="rplD"
                     /locus_tag="IXT21_000442"
     CDS             32293..32916
                     /gene="rplD"
                     /locus_tag="IXT21_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ4867876.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32916..33206
                     /gene="rplW"
                     /locus_tag="IXT21_000443"
     CDS             32916..33206
                     /gene="rplW"
                     /locus_tag="IXT21_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ4867877.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33245..34078
                     /gene="rplB"
                     /locus_tag="IXT21_000444"
     CDS             33245..34078
                     /gene="rplB"
                     /locus_tag="IXT21_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ4867878.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34121..34399
                     /gene="rpsS"
                     /locus_tag="IXT21_000445"
     CDS             34121..34399
                     /gene="rpsS"
                     /locus_tag="IXT21_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ4867879.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34422..34769
                     /gene="rplV"
                     /locus_tag="IXT21_000446"
     CDS             34422..34769
                     /gene="rplV"
                     /locus_tag="IXT21_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ4867880.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34783..35439
                     /gene="rpsC"
                     /locus_tag="IXT21_000447"
     CDS             34783..35439
                     /gene="rpsC"
                     /locus_tag="IXT21_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ4867881.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35442..35876
                     /gene="rplP"
                     /locus_tag="IXT21_000448"
     CDS             35442..35876
                     /gene="rplP"
                     /locus_tag="IXT21_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ4867882.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35866..36054
                     /gene="rpmC"
                     /locus_tag="IXT21_000449"
     CDS             35866..36054
                     /gene="rpmC"
                     /locus_tag="IXT21_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ4867883.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36079..36345
                     /gene="rpsQ"
                     /locus_tag="IXT21_000450"
     CDS             36079..36345
                     /gene="rpsQ"
                     /locus_tag="IXT21_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ4867884.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36403..36771
                     /gene="rplN"
                     /locus_tag="IXT21_000451"
     CDS             36403..36771
                     /gene="rplN"
                     /locus_tag="IXT21_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ4867885.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36808..37116
                     /gene="rplX"
                     /locus_tag="IXT21_000452"
     CDS             36808..37116
                     /gene="rplX"
                     /locus_tag="IXT21_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ4867886.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37144..37683
                     /gene="rplE"
                     /locus_tag="IXT21_000453"
     CDS             37144..37683
                     /gene="rplE"
                     /locus_tag="IXT21_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ4867887.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37702..37887
                     /locus_tag="IXT21_000454"
     CDS             37702..37887
                     /locus_tag="IXT21_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ4867888.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37923..38321
                     /gene="rpsH"
                     /locus_tag="IXT21_000455"
     CDS             37923..38321
                     /gene="rpsH"
                     /locus_tag="IXT21_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ4867889.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38353..38889
                     /gene="rplF"
                     /locus_tag="IXT21_000456"
     CDS             38353..38889
                     /gene="rplF"
                     /locus_tag="IXT21_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ4867890.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39050..39406
                     /gene="rplR"
                     /locus_tag="IXT21_000457"
     CDS             39050..39406
                     /gene="rplR"
                     /locus_tag="IXT21_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ4867891.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39427..39927
                     /gene="rpsE"
                     /locus_tag="IXT21_000458"
     CDS             39427..39927
                     /gene="rpsE"
                     /locus_tag="IXT21_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ4867892.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39942..40121
                     /gene="rpmD"
                     /locus_tag="IXT21_000459"
     CDS             39942..40121
                     /gene="rpmD"
                     /locus_tag="IXT21_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ4867893.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            40165..40605
                     /gene="rplO"
                     /locus_tag="IXT21_000460"
     CDS             40165..40605
                     /gene="rplO"
                     /locus_tag="IXT21_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ4867894.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40605..41900
                     /gene="secY"
                     /locus_tag="IXT21_000461"
     CDS             40605..41900
                     /gene="secY"
                     /locus_tag="IXT21_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ4867895.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41962..42609
                     /locus_tag="IXT21_000462"
     CDS             41962..42609
                     /locus_tag="IXT21_000462"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ4867896.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42800..43018
                     /gene="infA"
                     /locus_tag="IXT21_000463"
     CDS             42800..43018
                     /gene="infA"
                     /locus_tag="IXT21_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ4867897.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43051..43167
                     /gene="rpmJ"
                     /locus_tag="IXT21_000464"
     CDS             43051..43167
                     /gene="rpmJ"
                     /locus_tag="IXT21_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ4867898.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43185..43550
                     /gene="rpsM"
                     /locus_tag="IXT21_000465"
     CDS             43185..43550
                     /gene="rpsM"
                     /locus_tag="IXT21_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ4867899.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43578..43967
                     /gene="rpsK"
                     /locus_tag="IXT21_000466"
     CDS             43578..43967
                     /gene="rpsK"
                     /locus_tag="IXT21_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ4867900.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44047..44985
                     /locus_tag="IXT21_000467"
     CDS             44047..44985
                     /locus_tag="IXT21_000467"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ4867901.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45013..45393
                     /gene="rplQ"
                     /locus_tag="IXT21_000468"
     CDS             45013..45393
                     /gene="rplQ"
                     /locus_tag="IXT21_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ4867902.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45665..46504
                     /locus_tag="IXT21_000469"
     CDS             45665..46504
                     /locus_tag="IXT21_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4867903.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46480..47349
                     /locus_tag="IXT21_000470"
     CDS             46480..47349
                     /locus_tag="IXT21_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4867904.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47339..48136
                     /locus_tag="IXT21_000471"
     CDS             47339..48136
                     /locus_tag="IXT21_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ4867905.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48339..49085
                     /gene="truA"
                     /locus_tag="IXT21_000472"
     CDS             48339..49085
                     /gene="truA"
                     /locus_tag="IXT21_000472"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ4867906.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49418..52087)
                     /locus_tag="IXT21_000473"
     CDS             complement(49418..52087)
                     /locus_tag="IXT21_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ4867907.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52325..52513)
                     /locus_tag="IXT21_000474"
     CDS             complement(52325..52513)
                     /locus_tag="IXT21_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ4867908.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52783..53145
                     /locus_tag="IXT21_000475"
     CDS             52783..53145
                     /locus_tag="IXT21_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4867909.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53197..54879
                     /locus_tag="IXT21_000476"
     CDS             53197..54879
                     /locus_tag="IXT21_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ4867910.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54996..57032
                     /gene="recG"
                     /locus_tag="IXT21_000477"
     CDS             54996..57032
                     /gene="recG"
                     /locus_tag="IXT21_000477"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ4867911.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            57081..58082
                     /gene="plsX"
                     /locus_tag="IXT21_000478"
     CDS             57081..58082
                     /gene="plsX"
                     /locus_tag="IXT21_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ4867912.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58204..58446
                     /gene="acpP"
                     /locus_tag="IXT21_000479"
     CDS             58204..58446
                     /gene="acpP"
                     /locus_tag="IXT21_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ4867913.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58714..59718
                     /locus_tag="IXT21_000480"
     CDS             58714..59718
                     /locus_tag="IXT21_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867914.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59719..60663
                     /locus_tag="IXT21_000481"
     CDS             59719..60663
                     /locus_tag="IXT21_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867915.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60663..61625
                     /locus_tag="IXT21_000482"
     CDS             60663..61625
                     /locus_tag="IXT21_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4867916.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61652..62566
                     /locus_tag="IXT21_000483"
     CDS             61652..62566
                     /locus_tag="IXT21_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4867917.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62592..64373
                     /locus_tag="IXT21_000484"
     CDS             62592..64373
                     /locus_tag="IXT21_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4867918.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64721..65407
                     /locus_tag="IXT21_000485"
     CDS             64721..65407
                     /locus_tag="IXT21_000485"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ4867919.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65427..69008
                     /gene="smc"
                     /locus_tag="IXT21_000486"
     CDS             65427..69008
                     /gene="smc"
                     /locus_tag="IXT21_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ4867920.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            69017..69826
                     /locus_tag="IXT21_000487"
     CDS             69017..69826
                     /locus_tag="IXT21_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4867921.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69837..70835
                     /gene="ftsY"
                     /locus_tag="IXT21_000488"
     CDS             69837..70835
                     /gene="ftsY"
                     /locus_tag="IXT21_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ4867922.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            71022..73289
                     /gene="gshAB"
                     /locus_tag="IXT21_000489"
     CDS             71022..73289
                     /gene="gshAB"
                     /locus_tag="IXT21_000489"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ4867923.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73378..73830)
                     /locus_tag="IXT21_000490"
     CDS             complement(73378..73830)
                     /locus_tag="IXT21_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ4867924.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            73980..74930
                     /locus_tag="IXT21_000491"
     CDS             73980..74930
                     /locus_tag="IXT21_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ4867925.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74923..75882
                     /locus_tag="IXT21_000492"
     CDS             74923..75882
                     /locus_tag="IXT21_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ4867926.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75879..76634
                     /locus_tag="IXT21_000493"
     CDS             75879..76634
                     /locus_tag="IXT21_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4867927.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76657..77610
                     /locus_tag="IXT21_000494"
     CDS             76657..77610
                     /locus_tag="IXT21_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4867928.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        25517 a        14035 c        16634 g        21798 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg
     3841 gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct
     3901 acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg
     3961 ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt
     4021 ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca
     4081 gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat
     4141 tgtaaaatct gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt
     4201 cggatgctta ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta
     4261 gagccatatc ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac
     4321 gatgctccca aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt
     4381 attattggag aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt
     4441 acaaatgggc ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa
     4501 cctgtttgcc agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat
     4561 aaaataactt agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga
     4621 aatattcttt aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta
     4681 caagtggata ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac
     4741 tatacttaaa aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt
     4801 tcaaaaaatg aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt
     4861 tgagaaatga aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc
     4921 cttcaatatt tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt
     4981 tgtctgtaaa gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat
     5041 tatctgtagg taagaaaaat tcatcaatag atacatggag taatgataca agatcataca
     5101 gcacttgtgt acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa
     5161 tttcaatcat tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa
     5221 tcgctaaacc aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc
     5281 tctatacatt ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag
     5341 tttatgagtt cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata
     5401 gaaaggtcgc tgattttatt tacctataca actttataaa atttagtcat actcctagaa
     5461 agtagataaa attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca
     5521 tctagttctc ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat
     5581 gaccatcaat gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga
     5641 aaacaatctg tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc
     5701 atttatgcaa caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca
     5761 catgccactc cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa
     5821 gaaaccatag aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa
     5881 gattacttca aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat
     5941 aactaccttg tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt
     6001 aaattatttg gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat
     6061 ctttcagaga aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg
     6121 gagattgcgg agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga
     6181 ctagccatga ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg
     6241 ttcccttccc cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg
     6301 ttaaacatta tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg
     6361 gaaattctca tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct
     6421 ctaagatttt atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg
     6481 tatccacgct tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt
     6541 aggttttgat aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata
     6601 cgtttcggta agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg
     6661 attcatgtta gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg
     6721 tgataatgat actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt
     6781 acggctacat gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga
     6841 ttgctaagat attcttctct tgtcagtgta ttattataaa actatttgat ataattatag
     6901 gtgaatattg gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac
     6961 aaaaaaggat ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa
     7021 agcaaaagaa ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag
     7081 agtccctcgt gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc
     7141 tgagtagttt ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca
     7201 aagaatggaa cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt
     7261 caaaaatatc gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg
     7321 ctattgatga aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat
     7381 atctttgaaa ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat
     7441 cgcttaccaa ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt
     7501 gagcctattg atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat
     7561 gtaagagctg ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata
     7621 accaccacaa atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa
     7681 ttttggacaa aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat
     7741 caacactttt tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa
     7801 gctactgcca ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa
     7861 acactctatt acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc
     7921 gttcgtcata ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta
     7981 caacaaaatc taattcagac agactttgta atgagctata acggtattcc tacccaaaaa
     8041 catacactat catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga
     8101 atccatgggt tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt
     8161 atcattaaag atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat
     8221 ctttatccta acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa
     8281 gaagcaaaac aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga
     8341 aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac
     8401 ccttggtata agaggatttc tagctctcct acaactattc ccactccact gttgctggtg
     8461 gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta
     8521 cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga
     8581 ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac
     8641 cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag
     8701 cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt
     8761 cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa
     8821 cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg
     8881 tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt
     8941 gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc
     9001 cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa
     9061 catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca
     9121 acttgtctaa gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc
     9181 ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga
     9241 tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa
     9301 cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta
     9361 cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg
     9421 caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga
     9481 taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc
     9541 cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg
     9601 tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg
     9661 ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca
     9721 attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga
     9781 tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg
     9841 gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc
     9901 caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga
     9961 taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt
    10021 taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc
    10081 gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc
    10141 aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta
    10201 aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa
    10261 gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg
    10321 gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt
    10381 aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac
    10441 ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga
    10501 ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat
    10561 cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca
    10621 gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt
    10681 ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt
    10741 acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc
    10801 atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa
    10861 atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca
    10921 ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc
    10981 atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac
    11041 cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg
    11101 agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa
    11161 tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg
    11221 attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg
    11281 gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt
    11341 gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg
    11401 ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag
    11461 cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc
    11521 aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc
    11581 cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga
    11641 agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa
    11701 agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata
    11761 tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac
    11821 tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa
    11881 agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa
    11941 ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt
    12001 atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat
    12061 agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa
    12121 atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt
    12181 agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat
    12241 tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat
    12301 gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg
    12361 ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta
    12421 caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct
    12481 gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc
    12541 ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa
    12601 gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact
    12661 ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc
    12721 ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact
    12781 gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc
    12841 atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc
    12901 tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca
    12961 gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc
    13021 gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag
    13081 agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca
    13141 aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt
    13201 cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg
    13261 atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg
    13321 tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa
    13381 ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt
    13441 ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag
    13501 gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga
    13561 cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag
    13621 aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg
    13681 aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt
    13741 atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta
    13801 tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg
    13861 gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg
    13921 ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg
    13981 aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc
    14041 cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca
    14101 aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag
    14161 cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac
    14221 atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag
    14281 ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc
    14341 gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg
    14401 gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca
    14461 cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa
    14521 cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga
    14581 aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg
    14641 catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg
    14701 tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag
    14761 ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg
    14821 aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg
    14881 ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag
    14941 caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa
    15001 tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg
    15061 gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac
    15121 cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga
    15181 tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa
    15241 ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg
    15301 gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt
    15361 aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc
    15421 atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg
    15481 agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg
    15541 tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt
    15601 aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac
    15661 tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt
    15721 attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca
    15781 ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt
    15841 ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac
    15901 gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga
    15961 ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc
    16021 ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa
    16081 ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc
    16141 gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct
    16201 tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc
    16261 ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat
    16321 gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc
    16381 tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc
    16441 atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac
    16501 gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc
    16561 gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt
    16621 ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa
    16681 cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac
    16741 gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt
    16801 ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac
    16861 aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt
    16921 tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc
    16981 gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt
    17041 tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc
    17101 aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa
    17161 cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg
    17221 gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt
    17281 agggattggg atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa
    17341 catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa
    17401 agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg
    17461 tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc
    17521 agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt
    17581 aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa
    17641 gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat
    17701 cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag
    17761 aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat
    17821 tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga
    17881 ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt
    17941 gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga
    18001 agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga
    18061 agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca
    18121 aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga
    18181 acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc
    18241 aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga
    18301 tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt
    18361 tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt
    18421 cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat
    18481 tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg
    18541 ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc
    18601 gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct
    18661 taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc
    18721 tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt
    18781 tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc
    18841 tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag
    18901 atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga
    18961 tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa
    19021 caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc
    19081 ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc
    19141 ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag
    19201 caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa
    19261 tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta
    19321 cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag
    19381 gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg
    19441 gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct
    19501 tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg
    19561 agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc
    19621 gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag
    19681 aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt
    19741 aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact
    19801 aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt
    19861 catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat
    19921 gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca
    19981 cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct
    20041 gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga
    20101 taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat
    20161 tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt
    20221 cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga
    20281 aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt
    20341 actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg
    20401 taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt
    20461 tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag
    20521 cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca
    20581 aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg
    20641 tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg
    20701 gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa
    20761 aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga
    20821 attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac
    20881 attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc
    20941 cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa
    21001 gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc
    21061 aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa
    21121 gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac
    21181 gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct
    21241 gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc
    21301 caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt
    21361 ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg
    21421 aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct
    21481 gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa
    21541 agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc
    21601 agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc
    21661 tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa
    21721 gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc
    21781 gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg
    21841 gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt
    21901 tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga
    21961 gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata
    22021 aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag
    22081 atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga
    22141 cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag
    22201 ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag
    22261 tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg
    22321 tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg
    22381 gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga
    22441 gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt
    22501 tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg
    22561 agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag
    22621 tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag
    22681 cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca
    22741 aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat
    22801 aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg
    22861 ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac
    22921 aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt
    22981 caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg
    23041 tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat
    23101 gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat
    23161 ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag
    23221 atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc
    23281 tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa
    23341 atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg
    23401 atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct
    23461 acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat
    23521 gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt
    23581 ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt
    23641 tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg
    23701 tttgatgaat attgtgactt gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa
    23761 aaagggactg tgacaaatcg tgtcacagtc ccttttttat agtgtttttt ataggtagta
    23821 ttttttagca actgttaagt catcatttaa ttcataaaca agtggttgac ctgttggaat
    23881 ttcaagatcc atgatgtctt cgtcagagat accttcgata tgtttagcta gggcgcgtaa
    23941 tgagttacca tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg gagcaatttc
    24001 atcttgccag aatggcaaag cgcgttctaa tgtcactttc aagttttctc caccaggaat
    24061 atcgcgtttg tctaacattg cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc
    24121 ttccattaat ggaggaagag tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc
    24181 gtatttttca gctgtttctt ttttgttcaa accttgtaat ttaccatagt gacgttcgtt
    24241 taagcgccat gatttgattt gtggtaccca aagttgatct gaatattcta agatcaagtt
    24301 acatgttttg attgcacgag ttaaaacaga agtgtaagca acatcaaatt cgattccagc
    24361 ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg ctagatttac
    24421 atcagcccaa ccagtaaatt ggttcaatgc attccattca ctaagaccgt gacgagaaaa
    24481 aactaatttt ggcatgttta gctctccttt taagataggt ttatatttct gaaattttca
    24541 gttctgtttt attgtacaat tttttggaag agatttccat cgaaaaatta gataagattc
    24601 tatgagaaaa caagtagaga atataaaaaa acgaacagtg cccacgcaac tgttcgtttt
    24661 taggtattag atgcataatt gctcgatttc tttttcaggt aaattgatat atttataaca
    24721 tgttccaact gaaacaccgc attttgaaga aataaattgg atcgtttctt ttttttcata
    24781 atagagacgt cggatttttt tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt
    24841 tcctttttta cgagcttcat ctaatccaac taatgtacgt tctttgatca atgcgcactc
    24901 catgtttgct aggttttcca ttaattggaa atagattttt cccatttcgc gcgtatcgat
    24961 tccttcatca agactaatca agtgcagccc gcgatctttg aataattgcg tgagttctgt
    25021 taattgccgc gtagatttgc ctaatcgatg aagttggcaa atgactagtg catcgccttg
    25081 ttgcatgtca gcgatcactg actccaaatg aacagcagta gaatcatttt gttccacttc
    25141 atatgtctca tggaaaattt cttgacaacc aaagtcagat aattgatcaa cctgagttgt
    25201 taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata attttcatgg tgattccttt
    25261 ctagcagtga ttttctaact ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa
    25321 gatagttttt tcgcaaattt tccgctgtct ttttttattg gatgatcaag cagtttttct
    25381 ttatttacga cggattgcgt caagaatctc aacgcctttt tctgagccga caactacttt
    25441 agaaaccatg tttaggaaaa gcccatgttc tactacccca acaagttcat ctaaataggt
    25501 agctaagctt tctggctgtt cgatgacttc tagatgcaga tcaatgatgt aatgaccacc
    25561 atctgtggtt aagacttctc cgttttcgtc tgtacgcaag acgggttgga atcttttttc
    25621 ttcaaaaata tgaagtagct gttgggagcc ataagggatc acttcaactg ggagtgggaa
    25681 cttcccaagt ttatcgacta atttagaaga atcaacgatc caaatcgttt cttttgagta
    25741 cgtagcgact attttctcga aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc
    25801 agcactgatt tcatcagcac cgtctattgt caaatcgact acggggactt catcaatact
    25861 ctttaacggt attcctaatt tttccgcttg ttcttttgtc gcatttgaag tagtaactcc
    25921 tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc tcttctacca tataataggc
    25981 ggttgaaccg gtcccgagac ctacgatcat cccgtccttt acgaatttcg cggcttcaat
    26041 gccagctaat tgttttaaat tcatctttgc gtccaccttt ttttagtttg agcatcgtgc
    26101 tttcctcact attttagcgt gatttacggt aaaaagatac cattcttttt tattccaata
    26161 aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt ttttcattcg tgatattcta
    26221 acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat
    26281 atttgataag gatgcagaca ttgcatttat tttttatcac aaaaaaagaa ccacctggat
    26341 gtgtggaact agacgcaaat cgtgggaagg aggaaacaag gaatgcctac aattaaccaa
    26401 ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa
    26461 ggatataaca gctttaagaa aactcaaact aacgtaaact cacctcaaaa acgtggggta
    26521 gcaactcgtg tgggtacaat gacacctaaa aaaccgaact cagctttacg taaatatgca
    26581 cgtgttcgtt tgtcaaactt aattgaagtt actgcttata tcccagggat tggtcataac
    26641 ttacaagaac acagtgttgt attattacgc ggtggtcgtg taaaagactt accaggggta
    26701 cgttaccata tcgttcgcgg tgctttagat actgctggtg ttaacgaccg taaacaaagc
    26761 cgttctaaat acggaactaa aaaacctaaa gcttaatttt aattattaaa caaatgaaac
    26821 ttgcttctac tatatagaag taattactat gaacaatatt tcggaaggag gagttacgga
    26881 tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc agatcctatt tataactcaa
    26941 aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg aaaacgcggg attgcagcta
    27001 acattatcta taatgccttt gacttgatca aagaatctac aggtaacgat ccattagaag
    27061 tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga agttaaagca cgtcgtgttg
    27121 ggggttcaaa ctaccaagta ccagttgaag ttcgccctga acgccgcgtt actctaggtc
    27181 ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag
    27241 caaaagaaat catggatgct gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca
    27301 ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta tcgctggtaa gattctctct
    27361 gtttggtcgg cttttataag tcgacctcag atagtgtcct tatttttagc aactagatag
    27421 taaagagagg agaaacaagg agaatgacca gagaattttc tttacaaaac actcgtaata
    27481 tcggtatcat ggcccacatc gatgctggta aaacgactgc tactgaacgt atcttgtact
    27541 atactggtaa aatccataaa attggtgaaa ctcatgaagg agcttcacaa atggactgga
    27601 tggaacaaga acaagaacgt ggtattacga tcacttctgc tgctactaca gcacaatgga
    27661 agggtcaccg aatcaacatc atcgacacac caggacacgt agacttcaca gttgaagttg
    27721 aacgttcatt gcgtgtactt gatggtgctg taactgttct tgatgcacaa tcaggtgttg
    27781 aacctcaaac tgaaacagtt tggcgtcaag caacaactta tggtgtacct cgtatcgtat
    27841 ttgcaaacaa aatggacaag atcggtgctg acttcttata ttcagtatca actttacatg
    27901 accgtttgca agcaaacgct cacccaatcc aattaccaat tggtgcggaa gacgacttca
    27961 caggaattat cgaccttgta acaatgaagg ctgaaatgta tacaaacgat cttggaacag
    28021 agatcgaaga aacagaaatt cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa
    28081 aattggttga agctgtagct gaaactgatg aagaacttac tttgaaatac ttagaaggtg
    28141 aagaaatcac tgaagctgag ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat
    28201 tctacccagt actatgtggt tcagcattta agaacaaagg ggttcaatta ctattggacg
    28261 ctgttcttga ttacttgcca tcaccgttag atatcccagc tatcaaaggg attgacccta
    28321 aaactgatga agaagtagaa cgtcctgcag acgacagcgc tcctttctca gctttagcat
    28381 ttaaagttat gactgaccca ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg
    28441 ttttacaatc aggttcatac gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc
    28501 gtatcctaca gatgcatgcc aactctcgtt cagaaatctc tgaagtttac gctggagata
    28561 tcgctgctgc tgttggtttg aaagacacaa ctactgggga cacactatgt gatgaaaaga
    28621 gtctagttat tcttgaatcc atggaattcc cagaaccagt tatccaagtc gctattgaac
    28681 ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt acaaaaacta tctgaagaag
    28741 atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga aacaatcatc gctggtatgg
    28801 gtgaacttca cttagatatc atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta
    28861 acgtaggtgc gcctcaagta tcttaccgtg aaactttccg tgctggcact caagccgaag
    28921 gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg tcacgtatgg attgaattta
    28981 caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc
    29041 ctcgtgaata cattccagct gttgaaactg gtttgaaaga tgctatggaa aatggtgtct
    29101 tagctggcta tccattagta gatatcaaag ctaaacttta cgatggttca taccatgatg
    29161 tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat ggcattacgt gcagcagcta
    29221 agaaagctaa tccagtaatc cttgaaccaa tcatggctgt agaagttgtt atccctgaag
    29281 attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg
    29341 aagctcgcgc tggcggccaa caagttgttc gtgcgatggt tccacttgct gaaatgtttg
    29401 gttatgctac aactcttcgt tctgctactc aaggtcgtgg tacatttaca atgacatttg
    29461 accactacga agatgtacca aaatcagtac aagaagaaat cattaagaaa aacggcggaa
    29521 aagctgaata atttcttaaa accggatagt cctgcaaaga gattatccaa agagactata
    29581 tttttaatat agtttcgtgt aaaataatta tcgatgatat gggcatgtgg tgtaccttgt
    29641 gtacatcata tacctatcag acaaataaaa aatatattgt atttttagga ggacatttta
    29701 aaatggcaaa agaaaaattt gaccgttcta aaccacacgt taacattggt actatcggac
    29761 acgttgacca tggtaaaact acattaacag ctgcaatcac aactgtacta tctaagaaaa
    29821 acggcggcca agctatggct tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg
    29881 gaatcacaat ctcaacagct cacgttgagt atgaaacaga tacacgccac tacgctcacg
    29941 ttgactgccc aggacacgcg gattacgtta aaaacatgat cactggtgct gctcaaatgg
    30001 acggagctat cttggtagtt tctgctgctg acggcccaat gcctcaaact cgtgaacaca
    30061 tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac aaagtagaca
    30121 tggttgatga cgaagaatta ctagaattag ttgaaatgga agttcgtgac ctattaacag
    30181 aatacgaatt ccctggtgac gatgttcctg tagttgctgg atcagctttg aaagctctag
    30241 aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt gacgaataca
    30301 tcccaactcc agaacgtgac aacgacaaac cattcatgat gccagttgaa gacgtgttct
    30361 caattactgg acgtggtact gttgctacag gtcgtgttga acgtggacaa gttcgcgttg
    30421 gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca gttactggtg
    30481 ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg agacaacatt ggtgctttac
    30541 tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt tttagctaaa ccaggtacaa
    30601 tcacacctca tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac
    30661 gtcatactcc attcttcact aactaccgtc cacaattcta cttccgtaca actgacgtaa
    30721 caggtgttgt tgaattacca gaaggaactg aaatggttat gcctggcgac aacgtaacaa
    30781 tggaagttga attaatccac ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg
    30841 aaggcggacg tacagttggt gccggtgtcg ttacagaaat caaagcttaa ttcacttaag
    30901 ttttaaagta aagcaacatt tattcggacg taatcgctcg gaacgaaggt cttaggatct
    30961 ttgttccgag cttttttatt tattgcagga tcaggaaaaa agacaggagt attataaata
    31021 gaagaaacac gaaataaaag agtcagtatt ttctaaatga aactttatga aaacaaaatg
    31081 ttttttacaa gaaaaagcct tgctttgtga gtttatatat attataatga caaaggtgcg
    31141 tttactttag tgaattgcac agacgtaatc agaatgattt cgtcggctaa gaaacgagag
    31201 gttgcgacac gcccggacgc tttgccacga acgagcgtga cggaaatttt cgtggagcta
    31261 tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg caaaacaaaa aattcgtatc
    31321 cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag cggataaaat cgtggaaact
    31381 gcaaaaagaa ctggagctag cgtgtctggt ccaattccat taccaactga acgcagtctt
    31441 tacacaatca tccgtgcgac tcataaatac aaagattcac gcgaacaatt cgaaatgcgt
    31501 acacacaaac gtcttatcga cattgtgaac ccaacaccaa aaactgttga tgctttgatg
    31561 aagctagact taccatctgg tgtaaacatc gaaatcaaat tataaaacta aaacaaaaat
    31621 aatggaggtg tactcatgac caaaggaatc ttagggaaaa aagtgggaat gacacaaatc
    31681 ttcactgaat ctggtgaatt aattccagta actgttgtag aagctactcc aaacgtagta
    31741 ctacaagtga aaacaatgga aacggatggc tacgaagcta ttcaagttgg ttaccaagat
    31801 atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg ttgcaaaagc aaacacggct
    31861 cctaagcgct tcattcgtga attcaaaaat gttgagctag gagaatatga agtaggaaaa
    31921 gaaattacag tagatgtttt ccaagcagga gacattattg atgtaacagg tactacgaaa
    31981 ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa gccgcggacc aatggctcac
    32041 ggttctcgtt accatcgtcg tcctgggtca atgggtccag ttgcacctaa ccgtgtattt
    32101 aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg taactatcca aaacttggaa
    32161 gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca aaggtaatgt tcctggagct
    32221 aaaaaatctt taattactat caaatcagct gtgaaagcta aataattgta ggaagaaagg
    32281 aggaactaag gaatgccgaa tgtagcatta ttcaaacaag atggaagcca aaacggtgaa
    32341 atcacactta acgaagaaat cttcggaatt gaacctaatg aaagtgttgt ttacgatgca
    32401 atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg cggttaaaaa ccgtagcgct
    32461 gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa caggtcgtgc acgtcaagga
    32521 tctatccgct caccacaatg gcgtggaggt ggcgtagtct ttggaccaac accacgttct
    32581 tacagctaca aacttcctaa aaaagttcgt cgtttagcaa tgaaatctgt attgtcagaa
    32641 aaagttgctg aaaacaactt ggtagcaatc gaaggcttga acttcgacgc accaaaaaca
    32701 aaagaattca aacaagttct tgctaactta tctatcgact ctaaagtatt ggttgttcta
    32761 gaaaaaggta acgactttgc tgctttatca gcacgtaact taccaaacgt ttctgtagta
    32821 acttctgata atgtgagtgt gctagacgtt gtatcaaata caaaagtgtt agcaacacaa
    32881 actgctctta ctcaaattga ggaggtgctt gcataatgaa cttactagac gtaatcaaac
    32941 gcccagtgat cactgaaaaa tcaatgcttg ccatggatga caagaaatac acttttgaag
    33001 tggacactcg cgcaaacaaa actttagtaa aacaagcggt agaagctgct tttgacgtta
    33061 aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt caaacgtatg ggcaaatatg
    33121 caggatatac aaaaaaacgt cgcaaagcga tcgttacatt gactgaagat tcaaaagaaa
    33181 tccaattatt cgaagctgct gaataagcac taatagataa ctcattaaat taggagggaa
    33241 aaacgtggcg attaaaaagt acaaacctac ctcaaatggt cgtcgtaaca tgacaggttc
    33301 tgattttgct gaaatcacaa catcaacacc tgaaaaatca ttgttacaac cattgaaaaa
    33361 ccatgctgga cgtaacaaca acggccgcat cactgtacgt catcaaggtg gcggacacaa
    33421 acgccaatat cgcgtgatcg acttcaaacg taacaaagat aacgttgttg caactgttca
    33481 aacgatcgaa tatgatccaa accgttcagc taacatcgcg ttagtacact atgaagatgg
    33541 agtgaaagct tacatcttag cacctaaagg actagaagtg ggtatgaaac ttgtttcagg
    33601 tccagaagca gatatcaaag tcggaaatgc tcttccatta gaaaacattc cagttggtac
    33661 tgttatccac aacattgaaa tgaaacctgg taaaggtgga caattgatcc gttctgctgg
    33721 tacaagtgct caagtacttg gtaaagaagg caaatacgta ttgatccgct tgaactctgg
    33781 tgaagttcgt atgatcttag ctgcatgccg tgctacaatc ggttctgtag gtaacgaaca
    33841 acacgaatta atcaacatcg gtaaagctgg acgctctcgt tggatgcgta aacgcccaac
    33901 tgtacgtggt agcgtaatga accctaatga tcacccacac ggtggtggtg aaggtaaagc
    33961 tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa ccagctcttg gttacaaaac
    34021 tcgtaacaaa aaagctaaat cagacaaact tattgttcgt cgtcgcaaaa ataaataatt
    34081 actttgacca tgtgtcgcac attttgagag gaggttcacc atgggtcgta gtttaaagaa
    34141 aggacctttt gtcgatgagc atttaatgaa aaaagttgaa gctcaagcag gggttgaaaa
    34201 gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt ttcccaagtt ttgttggata
    34261 taccatcgca gtttatgatg gacgtaaaca tgtaccagtt tacatccaag aagacatggt
    34321 aggacacaaa ttaggtgaat ttgcacctac aagaacttat cgtggacatg ctgctgacga
    34381 taagaaaacg aaacgctaat ttgaggggag gataaatcca aatggcagaa caaatcacat
    34441 cagctaaagc aactgcaaaa acagttcgca cttcacctcg aaaagcccgt ttagtaattg
    34501 accttatcag aggtaaaagt gtcgcagatg caatttcaat cttgaaattc acaccaaaca
    34561 aatctgctgg aatcattgaa aaagtattga tgtcagcagt tgcgaatgca gaaaataact
    34621 ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt tgttaacgaa ggacctacaa
    34681 tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc aatcaacaaa cgtacaagtc
    34741 atatcacggt agtcgtatca gaaaaataag gagggataat cagtgggtca aaaagtacat
    34801 ccaattggaa tgcgtgtagg catcatccgc gactgggatg ctaaatggta tgctgaaaaa
    34861 gagtatgctg agttcttaca cgaagattta agaatccgta aatttatttc aactaaactt
    34921 gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa caggtcgcgt aaacatttca
    34981 attcacacag ctaaaccagg tatggttatt ggtaaaggcg gttctgaagt cgaaagccta
    35041 agaaaagaat taaacaaatt aactggtaaa agaatccata tcaatatcgt ggaaatcaaa
    35101 aaaccagatc tagatgcaaa attagtaggt gaaggaatcg cacgtcaatt agaaaaccgt
    35161 gttgctttcc gtcgcgctca aaaacaagcc atccaacgct caatgcgtgc tggcgctcaa
    35221 gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag atatcgctcg ttcagaagga
    35281 tactcagaag gaactgttcc tcttcatact ttacgtgcgg acattgatta cgcatgggaa
    35341 gaagcagata caacatacgg aaaactagga gttaaagtgt ggatctatcg tggagaaatt
    35401 cttccaacta aaaaaaacac tgagaaagga gggaaataat catgttagta cctaaacgtg
    35461 taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga agctaaaggc ggaaaagaag
    35521 tagcattcgg tgaatacggt ttgcaagctg ttgattcaca ttggatcaca aaccgccaaa
    35581 tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg tggtgggaaa gtatggatta
    35641 aaattttccc tcacaaatct tatactgcca aagcaattgg ggtacgtatg ggttctggta
    35701 aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg taaaatcatg tttgaaatcg
    35761 caggcgttcc tgaagaagta gctcgtgaag cgttacgtct agcttctcac aaattaccaa
    35821 tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga atcgaatgaa ggttaaagaa
    35881 atcagagaat taaccactgc cgaaatgctt gatcaagaaa aacaattaaa agaagaattg
    35941 tttaatctta gattccaatt agcaacaggt caattagaaa acactgcacg tattaaagaa
    36001 gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg aacaagctaa gtaattatgg
    36061 aaggaggcca ttaagcgtat gactgaagga agaaatcaac gcaaagttta tcaaggtcgc
    36121 gtggtttcag acaaaatgga taaaacaatt accgttgctg tcgaaacaaa gaaaaaccac
    36181 cctatctatg gcaaacgcat gaactattct aagaaataca aagtgcatga tgaaaacaac
    36241 acagcaaaag ttggcgacat cgtgagaatt atggaaactc gtccattatc agctacaaaa
    36301 cgtttccgtt tgttggaagt agtcgaagaa gcagttatta tctaataaca cgagattttc
    36361 ctatatagat tgaatgcaaa atctgaaagg aggatacaca acgtgatcca agcagaaagt
    36421 cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt tgacgatcaa agtccttggt
    36481 ggatctggtc gtaaaactgc taacatcggt gatgtgatcg ttgctacggt taaacaagca
    36541 acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag ccgttatcgt tcgaacaaaa
    36601 tcaggagctc gtcgtacaga cggttcatat attaaatttg atgaaaatgc tgcagtgatt
    36661 attcgtgatg ataaaagccc tcgtggaaca cgtatctttg ggccagttgc acgtgaacta
    36721 cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag aagttttata atcttgagac
    36781 acttatcaaa ggaggtgcga aacagttatg tttgttaaaa aaggcgataa agttaaagtt
    36841 atcactggaa aagacaaaaa caaagaaggc gttgtactag cagcgttccc gaaaaaagat
    36901 aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc aaaaaccttc tcaagctgct
    36961 cctcaaggtg gaattgttga gatggaagcg ccaattcatg tttctaacgt gatggtggtt
    37021 gactctactg gtgtagctgg ccgagttggc tacaaagaag tagacggcaa aaaagtccgt
    37081 gtttctaaaa aaaccggtga agttttagat aaataaaagt ttaaggaagg agggtcttac
    37141 tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa ctccatcatt gatggaaaaa
    37201 tttaactata gctctgttat gcaaacacct aaagttgaaa aaatcgtcat caacatgggt
    37261 gttggtgatg cggtatcaaa cgctaaaaac ttagataaag cagttgaaga attaactttg
    37321 atcactggtc aaaaaccaat gattacaaaa gctaaaaaat caatcgctgg attccgttta
    37381 cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg gagaaagaat gtacgaattc
    37441 ttagacaaat tggtttctgt ttcactacca cgtgtacgtg acttccatgg tgtaagtaaa
    37501 aaagcctttg acggtcgtgg aaactatact ttaggtatta aagaacaatt gatcttccca
    37561 gaagtagatt atgatttagt agataaagta cgtggtatgg acatcgttat tgtaacaaca
    37621 gcaaactctg atgaagaatc tcgtgagttg ttgacacaat taggcatgcc attccaaaaa
    37681 taattaaagg aggcgaacta cgtggctaaa aaatcaatga ttgctaaaaa caaacgtcct
    37741 gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt gcggacgtcc acattcagtt
    37801 tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac ttgcctataa aggtcaaatt
    37861 cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc ataagaagga ggtaaaagat
    37921 caatggtcat gacagatcca attgcagatt ttctaactcg tatccgtaac gcaaacatgg
    37981 ttaaacatga gtctttagaa gtacctgcat caaaaattaa acgtgacatc gctgaaatct
    38041 tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga agatgacaaa caaggcgtga
    38101 tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt tatcacaaac ttaaaacgta
    38161 tctctaaacc aggtttgcgt gcttatgtca aagctgacga agtaccaaaa gtattgaacg
    38221 gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt cactgataaa gaagctcgtg
    38281 aaaaaaatat cggcggcgaa gttatcgctt acgtatggta atcaagtaga atctacaagg
    38341 aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg ttatccctga aggagtaaca
    38401 gttactcaag acggaaacaa cgttacagtt aaaggaccaa aaggtgaatt gactcgtact
    38461 ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag taactttcac tcgtccaaac
    38521 gacagtaaag aaatgaaaac aattcatgga acaactcgtg cgaacttcaa caacatggtt
    38581 gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa tcggggttgg gtaccgtgca
    38641 caattacaag gatcaaaact tgtattgaac gttggttact cacatccagt agaaatcaca
    38701 ccaccagctg gagtaactgt tgaagtacca gctaacacac aagtaattgt taaaggtgcg
    38761 aacaaagaag aagttggcga attagctgca aacattcgtg gaactcgtcc tccagaacct
    38821 tataaaggta aaggtattcg ttatgttggc gaatatgtac gccgtaaaga aggtaaaact
    38881 ggtaaataat cgtttagaaa aattgcatag aagccagcag caggaaaaag gcatttggct
    38941 gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc agcttctatt tttcgaaacg
    39001 tatttactgc ttgaaatagt agtacaataa aaataaagag gtgacaattg tgattacaaa
    39061 accagataag aacaaaacac gtcaaaagag acatcgtcgt gtacgtaaca atatctctgg
    39121 tactgctgag cgcccacgct tgaacgtttt tcgttctaac aaaaacatct acgctcaaat
    39181 tattgatgac gtagcgggtg taacgctagc aagtgcctct accttggata aagaaatttc
    39241 aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa ctagttgctg aacgtgcagc
    39301 tgaaaaaggc attaaagtag tagtctttga ccgtggtgga tacctttacc atggccgtgt
    39361 gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa ttttagaaga aggaggaata
    39421 ccattcatgg tttatatcga tccaaaacat ttggaattag aagaccgcgt tgttgcaatt
    39481 aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac gttttgctgc tttagttgtt
    39541 gtcggtgaca aaaacggaca cgtagggttt ggtacaggta aagctcaaga agttcctgaa
    39601 gcaatccgta aagcaattga agatgcgaag aaaaacttag ttgaagtacc tatggttggt
    39661 tcaacaatcc cacacgaagt aatcggtgct tttagtggtg gtagaatcct aatgaaacct
    39721 gctgtagaag gttctggggt tgctgctggt ggaccagttc gtgccgtatt agaattagct
    39781 ggggtagcag atatcacttc taaatcttta ggctcaaata caccaatcaa cgttgttcgc
    39841 gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag aagttgcaga acttcgcggc
    39901 aaatcagttg aagaattaat cggataagga ggacaacaat aatggctgaa ttaaaagtta
    39961 ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg cgatacagtt aaagcgttag
    40021 gtcttacaaa gattaacagt tcagtggtta agccttctaa tgaagcaatt aaaggcatga
    40081 tcaatactgt ttctcaccta gtggacgttg aagaaattta atttagaaaa atcataacta
    40141 gaacattaag gaggtgccaa acctatgaaa cttcatgaat taaaacctgc tgaaggttca
    40201 cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg gtaacggaaa aactgccgga
    40261 cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg tgcgtttagg atttgaaggg
    40321 ggacaaacac cattgttccg tcgtttacca aaacgaggat tcacaaacgt gaaccgtaaa
    40381 gactatgcag tcattaatct tgatgtattg aaccgctttg aagacggagc tgaagtaact
    40441 cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa aagctggaat caaagtatta
    40501 gctaatgggg aactaaacaa gaaattaact gtgaaagcag caaaattctc taaagcagct
    40561 caagaagcaa ttgaagcggc tggtggatca atcgaggtga tctaatgttt agactattaa
    40621 aagatgcttt taaagtaaaa gacattagat cgaaaatcct atttactgta tttgtgttgt
    40681 ttgtatttcg cctaggtgca catattactg taccaggcgt aaatgctgct tcattgcaaa
    40741 acctttcttc tttgccattt ttcaacatgt tgaacttggt aagtggtagt gcgatgcaaa
    40801 acttttccat tttctcaatg ggggtttcac catacattac tgcttctatc gtcattcagc
    40861 ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc gaaacaaggg gaagttggtc
    40921 gtaaaaaact gaatcaagcg actcgatatc tgacattagt attggcattt atccaatctg
    40981 tcggtatcac tgctggattc aatcaatacg ctcaattagg tttcgtgaat aatcctaacg
    41041 tagcaactta cgtaacgatc ggtttgattc taacagccgg tacgatgttt gttacttggc
    41101 taggagaaca gatcacagaa aaaggtatcg gaaatggcgt atcaatgatc atttttgcag
    41161 gtatcatttc tagattgcct gaaagtatca gagaaattta tgaagattac tttataaata
    41221 ttgatccttc agacatatgg aaatctgcta tttttgtggc cattttacta gtagcggtct
    41281 tgttgatcgt gacattcgta acatttttcc aacaagctga acgcaagatc ccgatccaat
    41341 atacgaaacg cgtagctggc gcgcctacaa gcagctatct gccgttgaaa gtaaatgcag
    41401 ctggagttat tccagttatc tttgctagtt cgtttattgc tacgccaaat gctgttttac
    41461 aagcattgag cggtagattc ggttctgaaa actggtacaa agtgatgatg gagatcttta
    41521 actataatac agcacctggt gctacgattt atacgattct gatcgttgcc ttcactttct
    41581 tctacgcctt tgttcaagta aaccctgaga aattagcgga aaacttgcaa aagcaaggaa
    41641 gttatatacc aagcgtacgc cctggtaagg ggactgaaga gtatgtctca agattactga
    41701 tgcgtttaag tacagttggt gcagtcttcc ttggtttagt ggcattgctt ccaatcattg
    41761 ctcaaatggt ttgggatctt ccacaatcaa tcggattagg cggtacaagc ttactgatcg
    41821 ttatcggtgt agctttagaa actgcgaaac agttagaagg cttaatgctg aaacgtaaat
    41881 atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc cctcaacaca cgttctcaca
    41941 catttattag gagggaaaac aatgaacctc attttaatgg ggctgccagg cgcaggtaaa
    42001 ggaacacaag ctgaaaaaat cattgatgct tatggtattc cgcacatctc aacaggagat
    42061 atgttccgcg cagcgatgca aaacgaaaca gctcttggcc tggaagcaaa gtcttatatg
    42121 gataaaggtg cattagttcc tgatgaagta acaaatggaa tcgtgaaaga gcgactagct
    42181 gaaccagata cagagaaagg cttcttatta gatggctttc cacgtacgct agatcaagca
    42241 aaagctctgg acgcaatgct aaaagatttg aataaaaaaa ttgatgctgt catcgatatc
    42301 catgttggtg aagaaatctt ggtcgaacgt ctagccggcc gatttatctg cagcaattgt
    42361 ggagcaactt accataaaat tttcaaccct acaaaagttg aagacacatg tgatcgttgc
    42421 ggtgggcatg aattctatca aagagaagat gataaacctg agacggttaa aaatcgtctg
    42481 gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt ataaagaaca aggtttgcta
    42541 aacactattg atggtgctcg tgaaatcgat gctgtatttg cagatgtaca aaaaatcatt
    42601 gaaaaatagc acgattgccc gtagccgact tgcattatgc agttactctt gtcaagttca
    42661 ataaattatg atagaatatg tcagtccgac ttctagagaa tcctctagat gatccatttt
    42721 gagaattttg aggtgggaac tgcagtttcc gccattttat cgtgtgaaaa tgcgaaacaa
    42781 gtacaaggag gtactgtgcg tggcaaaaga agatatgatt gaagtcgaag gtacagtcgt
    42841 cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa aacggacacc aagttcttgc
    42901 tactgtttca ggtaaaattc gtatgcatta catccgtatc ttacctggtg acaaagtaac
    42961 ggttgaattg tctccatacg atcttaaccg tggccgtatt acttatcgct ttaaataatt
    43021 gtactccgta aaactacagg aggtattatc atgaaagtaa gaccatcagt aaaaccaatg
    43081 tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta tggtgatttg cccagcaaat
    43141 ccaaaacata aacaacgtca aggataattg gaggtgctat aaatatggct cgtattgcag
    43201 gagtagatat tcctcgtgat aaacgtgtag tagtttcact tacttatatc tatggtattg
    43261 gtaacactac tgctaaaaag atcttagctg atgctggtgt atctgaagat gttcgcgttc
    43321 gtgaattaac gaatgaacaa acagatgcta tccgtgcaga agtagataaa ttaaaagttg
    43381 aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg tttgatggaa atcggttctt
    43441 accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg acaaaacacg aaaaacaatg
    43501 cacgtactcg taaaggccca gctcgcgcta tcgctggcaa gaaaaaataa ttcttaagtg
    43561 aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg taaacgccgt gtgaagaaga
    43621 atatagaagc aggtattgca catatccatt ctacattcaa taacactatc atcatgatca
    43681 ctgatactca cggaaatgca ttagcgtggt catcagctgg ttcattaggt ttcaaaggaa
    43741 gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga aactgctaca aaagcagcaa
    43801 tggaacacgg attaaagact gttgaagtaa cagttaaagg acctggttct ggacgtgaag
    43861 cagcaattcg ttcattacaa gctacaggtt tagaagtgac tgcaattcgt gacgtgactc
    43921 cagttcctca taatggtgcc cgccctccaa aacgccgtcg tgtttaatga gtgccgctca
    43981 ttttgagttt tatcagtaac gtcattgaac aagacacttt tcgttttgaa aggggtaaag
    44041 ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa ttgatgaaga aaaagattat
    44101 ggcaagttca tcgttgaacc tcttgaaaga gggtatggga ctactttagg gaattcccta
    44161 cgtcgtattt tattatcttc tttgccaggt gctgccatca ctaatattca aatcgatggt
    44221 gtactgcatg aattctctac catcaaaggt gttagagaag acgtcactca aatcatcttg
    44281 aacattaaag gcttagcgct gaaaatgtat ggccaagaag aaaaaaccct tgaaatcgat
    44341 attaccggac ctgctacagt aactgctggc gatattatcg ttgatagtga tgtagaaatt
    44401 cttaacaaag atatgtacat ctgtagtgtc tctgaaggag ctacgttcca tgctcgctta
    44461 acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa ataaaaaaga agatatgcca
    44521 attggcgttc ttccagtcga ttcaatctac acacctgttc gtcgtgtgaa ctatcaagtt
    44581 gagaacacac gtgttggaca ccgtgaagat tttgataaat tgacgatgga gatttggaca
    44641 gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg cgaaaatcat gactgaacat
    44701 ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg ctgaaatcat ggtcgaaaaa
    44761 gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg aagagttaga cttgtctgtt
    44821 cgctcttaca attgtttgaa acgtgcagga atcaatactg tacaagaact tacaaataaa
    44881 tctgagcctg aaatgatcaa agtccgtaac ttaggacgta aatcattaga agaagttaaa
    44941 gcaaaattgc acgacttagg tttaggttta cgtaaagaag attaaaacct tttacgaagg
    45001 agggaaacca acgtgagtta tcgtaaacta ggacgcacat ctagccaacg taaagcgatg
    45061 ttgcgtgatt taacaactga tttattaatc aacgaacgta ttgtgactac tgaagctcgt
    45121 gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt taggtaaacg cggagatttg
    45181 catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag ttgccagcgt gcgtgaagaa
    45241 aatgaggaca tcgtcattga atcagcttta caaaaactgt tcaacgatat tgcacctcgt
    45301 tacgcagaac gtcaaggcgg atacacacgc attttgaaga cagaaccaag acgcggtgat
    45361 ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca ctaataacat cactttatag
    45421 atgattcttt tagagtgtta tgatgttgga agatattttt ccgagtctag ctcagcgctg
    45481 ctcctcaagc gaatatcgct tggggagtaa gcactcatca taaagtgttt ttttatacct
    45541 tcaattacct gtcatgacag atatgtttga aggcataaaa aaacatttta agcgagaaat
    45601 aattgaaaat tttgtctttt tttgatagtg ttagtaggaa ctttaggatg aagaaggagt
    45661 aatcatggag ccaattattg aattagaaaa gataaattat aaataccagc cagatgatct
    45721 tcgccccgca ttgaaagatg tctcctttac aattgataaa ggtgagtgga tcgctattat
    45781 tggacacaat ggttcaggaa aatcgacact tgccaaaacg atcaatggat tgcttctgcc
    45841 agaatcaggg atcgtgaaag tgggaaatca aatattggat gaagaaaata tttggacgat
    45901 ccgacagatg gttggaatga tttttcaaaa tccagacaat cagtttgttg gttcgacagt
    45961 agaagatgac gttgcttttg gtcttgaaaa tcaagggatt cccagagaag aaatgctagt
    46021 gcgtgtgaaa gatgcacttg agaaagttag aatggctgag tttgcttcac gcgagcctgc
    46081 tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc
    46141 tgatatcatt attttagatg aagcaaccag tatgctagat ccagaaggtc gtgaagaagt
    46201 gatctctacg atcaagaaga tcaaagaaga aagtcaattg accgttatat ctatcactca
    46261 tgatattgat gaagcagcta atgccaatcg gatactagtg atgagacaag gcgaacttgt
    46321 ccgtgaaggt acaccaaaag aaattttttc tgctggtcct gaactgatcg acttaggtct
    46381 agatctacca tttcctgaaa aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc
    46441 cagtgaatat atgacagaag aaaggatggt ggattggtta tggacatccg ttttgaacaa
    46501 gtagacttca cttatcagcc gaatacacca tttgaacaaa gagccttatt tgacatcaat
    46561 atgacgatca aagaaaacag ttatactgca ttagtagggc ataccggaag cgggaaatca
    46621 accttacttc agcatttgaa tgcgcttgtt aagccaacaa gcggtacagt ccatattgga
    46681 gaacgagata ttcagccgga tacggataat aagaatttaa agcccatccg aaaaaaagtt
    46741 ggcatcgtct ttcagtttcc agaagcacag ctattcgaag aaacggtagc aaaagacatt
    46801 gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag cactagtcct agcaaaagaa
    46861 acattagaac aagttgggct ggatgaaagc tatttggaac gttcgccatt tgaactttca
    46921 ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt
    46981 gtattggatg aaccaacggc aggacttgat ccccagggca gaaaagaaat gatggagatg
    47041 ttttggcgtc ttcataagga acagcagatc actattgtat tagttacgca tttaatggat
    47101 gatgtagcca attttgcaga ttatgtctat gtgttggaaa agggacgaat cgttaatagc
    47161 ggggagccgc aagaggtctt tcaaaacatt gaatggctaa aagaaaaaca actaggcgta
    47221 cctactgcca ccgagtttgc agaagaatta atggctaaag ggatgaactt tgctaccttg
    47281 cctttgacag ctgaagaatt agcagacgct atcgtccatt atgctggagg aaatgcccat
    47341 gatgaataaa ttgatatttg gtcgttatat gcctggagat tccttgatcc atcgattgga
    47401 tccccgagct aaattattag ccagttttta ttttatcgga atcatttttt tagcaaataa
    47461 ttgggaaacg taccttttgt tggcattttt cacattactt tctgtcttct tgtcaaaagt
    47521 agatttgaaa ttttttatcc gaggcattcg cccattgatc tggctaatcc tcttcactgt
    47581 agctttgcag gtcttgttta caagtggcgg agaaacttat tggagttggg gtgttttcca
    47641 tatcactgaa tttggtgtga tgaacggcct ctttattttc tgtcgattcg tgttgatcat
    47701 ttttatgtcg actctactca cgcttactac accgcctttg gaattatcgg atgcgattga
    47761 gtacttgctt cgtccattga aagccgtacg gttcccagtc cacgaaattt cattgatgct
    47821 gtcgattgct cttcgttttg tgcctacatt gatggacgaa acagaaaaga tcatgaacgc
    47881 tcagcgcgct cgcggtgttg attttggtga aggaaaccta gttcagaaaa tgaaagcagt
    47941 cattcctttg ctgattcctt tatttgtcag cagcttcaac cgtgcggaag atcttgcgac
    48001 tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca
    48061 ttggcataat agagacacga tggttatcat tgcttttgta ttattaactg tgctactgat
    48121 ctttttaaga ggataaacgt tcagtgggcc gctatctatt cttttcttgc tgatctgttt
    48181 gcaagaaagg atgcaagcag tgtatgacaa gaaatattag ggtctgtggc agactaaggt
    48241 cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa aattttatag aaaaatcatt
    48301 attccagata accttttagg aacaaaggag agaaaaacat ggtacgttac aaagcaatca
    48361 ttgcgtacga cggaacaaat tttaatggat tccaaaaaca gccaaatggg cgtactgttc
    48421 aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg aaaagaaatc acggtctttg
    48481 gttcgggaag gacagatgcc ggagtacatg cgatcgggca agtaatccat tttgattatc
    48541 cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga tacccagtca ccagaggata
    48601 ttgctgtaag acaagtagaa attgtttcag aagacttcca cgcaagatat ttggtcaaag
    48661 aaaagacgta tcaatttcga gtggatatag ggaaaccacg cagtcctttt cgacgccatt
    48721 atgcaagtta ttttccttac ccacttgatt tggaaaaaat tcagcgggcg cttcctgatt
    48781 tattagggac tcacgatttc acctcttttt gtgcatcagg aagctcgata gaggataaaa
    48841 tacgtacgat ctacgaggct aagatggagg taaatgaaac aaaagacgaa ttgttgttta
    48901 catttagggg aaatggcttc ttgtataaga tgatacggat cttagtagga acgcttttga
    48961 aaattggcaa tggccgattg cctgaagata gtatcccaga tattattgcg aaaaaagatc
    49021 gtaatgcagc aggacctaca gcacaccctg aaggacttta tttgtatgaa gtgaaatatg
    49081 aataagaggt tatgatagaa gtggtcagct tcaagaaata agaaggtatt tacgaaaatt
    49141 acttctcaaa attttgcaaa atttcggctt attttcgaag aagctacttc tggaacaccg
    49201 tttaagaaag aggtcgtgag cctgtcattt aactctgagt cacaaagagc aatttcaaaa
    49261 aacagcttct catctttgat gaatatcgtg acttgtggac gaggcgttga taagtgaatc
    49321 atctttttgt tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc
    49381 cttggtgcgg acagtctgaa caggttccat cgtttcttta ttttctacgt cttcttctgt
    49441 gtgtgtttcg tggttgttct tctattgctg gatcaatcgt gccataaaag gggcgattgc
    49501 ggtcaaggta ccagacataa agaataaaca atgcaataca taggacaaaa aggattgcac
    49561 ctatcgtaaa tccttcaggc aagtaactga aagtgacagt atgtttacct gctggaactt
    49621 ttatcatcaa gaatgcgtct ttgaaattgc tgaccttgac tggtttccca tcaattttag
    49681 ctttccatcc tttgtcataa ggaatagttg tcaccaacat ggaatcttca gcggtattta
    49741 cagtcccaga agcttttctg ccagtggctt ccatttcgac acctttttct ttgatctgat
    49801 tcattgcggc ttgataggct acaacatcta gaccaacgac tttaggctcc atgaaactta
    49861 tttctttcgt cccataaaaa cttgcagtaa atgtcactgt tgtctcttga ggataatagc
    49921 caagatcata gtattgacca gaaatgttga tttgcgtttt tcgcgatgta ccattgacag
    49981 tgatcgtcgc actagaactt tctaattgcg caaaattggt tgggaacaaa cttaagtaag
    50041 cttgtgtatt tgcaggcaca tggacttccc aagtgagctc ttttggctgg ttatctgctg
    50101 tttctgagta agtgacgcca gtcgttgtct ttgtgatcgt cgtattcaac tgattcttca
    50161 aagtaggtgt atagaatgta aaatactgtt catttagacc agacaatgta ttgatcaagt
    50221 ttgtctgact ggtcaaatta tctgtcactg gctgctcaac atcattaacg gacaacggtg
    50281 cagtaaatgc tagtggcaat gcattgacat tctgatacag tgtatagtcg ccgctttgac
    50341 cagcttggcg gaagccatat ttactgaacg tgctttcgtt tttagaaatg ttatatttga
    50401 ttcctgtaaa cccatccatc agcaaagtat tattgttata acggatattc aaatttgtcc
    50461 ctcttgaacg gaaacctaat ttatccaggt aagaggaaga attacgattt cttatagaag
    50521 aaaacaaact gataccactg taaccatagt tgatcgaatc gtttgcagat actggatcca
    50581 gattttccaa gcggtaaaac gagtcggaat ctttttttgt ttcatttaca agagctttca
    50641 agtcaggata aggatcagta tataaactgc gggaagcata attccaatca tcaagaatgc
    50701 catggatcat actgttcgta ttgaccagtg cttcagcaga catgatcaat aataacaata
    50761 tactcagatg attgaatgaa agttttttat gctgataaaa tcccacagca aaagcataaa
    50821 tccctaagaa aaccactgtc aatacaaagg aagtcatcgt cacataggtg tagcttcctt
    50881 caggtttgat gccccatgcc accgcaaaga gagcagctac gaccaaaatg attcctaaca
    50941 acagcccacc atcgtcttgt tctatctttt cccatccttt tcctgcaagc aggagcacta
    51001 agaaagagaa cagataggca taacggaata aaaacatatt cggtgcatgc ataccatgcc
    51061 agaacaaatt caatggaaca agataaaaac ttgcgataag tatggcgaaa agactgccgt
    51121 acaaaaattt atttttcaac ggtactttcc gactggcaaa ataaaacaga cagaggacta
    51181 atggtagcag acctgcgtaa acgaacggaa tcgatccgta ttttgtcgtg tcataaacac
    51241 caatcatatt tttcatgatt agatccagat aagaagtcgc ttctgttttc aacgtagtga
    51301 tctgactcag tgcctcgcca tttgaccgaa gatccagcac tgctggcaat acaatgatca
    51361 ttgaagcgcc gcctgccagt aaagaagtga taccataagg aacaatcgtt tttttatatt
    51421 ctgaccagtt tgtcaataga cgggcgataa aatataagaa agaaaatacg ccaatcataa
    51481 aacccatata aaaactggat aaaaacaaga gtaaataact gataaataaa agtttcggcc
    51541 gtttaagatc cataacacga tgaatcccca aaatcactaa tggaagataa acaaatgcat
    51601 ccagccacat gatgatctcc gaatgtgcag tgataaatga gatcaatgcg taactgacgc
    51661 taagtgcaac atgcccccag cgaggaatct tgaacgtacg tgcaagaaac caaaaagcca
    51721 atccggcaca gccgacctta agcaaagtca gaacatacag tgcatctggc atcatctggt
    51781 ttggaaaaaa gaacaccagt ggcgtgaaga tcccgcctag atagtaagaa attaaagata
    51841 aataattcaa tcctagagaa gcattccatg tataaaaaat actttgcttt ccaagaagaa
    51901 catttcggaa gctggcatga aaattagaaa actgtgaaaa ggcatcactt gccaaaatac
    51961 ttctgctgct tccaggatag attccgatac ttagataaac aagtgctaaa atcaaaaacg
    52021 gaatcaggaa actagctgct gcgtagcgtc cgttttctct aaagaatgac tttaaatttt
    52081 ttttcatatt tattttccac caaatttcta tgaagttgta ttcagtttat catatccttg
    52141 tttttctctg cttaaaaaga gtctagtttt cacaaaaaaa taaaatttag aataaataac
    52201 ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc
    52261 acccttaaaa atttttatta aataaaaaac cgttcactat ctgaacggtc caagtatcat
    52321 tcaattaaac gcgttcaact tttcctgatt tcaaagcacg agttgacacc caaacttttt
    52381 taggttttcc gtcgaccata acgcgaactt tttgtaagtt aggtttaaca gtacgttttg
    52441 ttgagttcat agcgtgtgaa cggttgtttc cactttttgt cttgcgacct gtaaagtaac
    52501 aaacttttgc catggtaatt tcctcctttg ccttgatttg gagcaataga ataattcagc
    52561 tcataccaga ctaacatatc atatagaatg ggaatttgca agtacatttt catagataca
    52621 attgacaatt gagaagaata attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg
    52681 tttttgagta ctttgggtaa ctgcctttta tttcaagttg cgctatgata aaatatgata
    52741 gaagaaaaat gggagttttt tacccgttaa ggaggctcat tcatggctgt aaaaattcaa
    52801 acgtcagctg gaaccattga aattaccaat gatgtaattg caacagttgt tggtggcgcc
    52861 gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa agataatatt
    52921 actgagattt tgcgtaaaga aaattactca agaggagtag ttgtccgcca agaagataat
    52981 ggtattgctg tagatgttta tacgattatc agctatggta cgaaaatctc tgaagtttcc
    53041 cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat tattaggcgt aactgccaat
    53101 tcagttaatg tctttgtaca aggtgttcgt gtactgccag actaggcagg agactggctg
    53161 gtaactcagc agtcgatctg aaggaggatt atctaggtga gtgtaacaga aataagtgca
    53221 agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac
    53281 gtcaattcat taaatgtatt ccctgtgcca gatggcgata cgggaacaaa tatgaatcta
    53341 tctatgacta gtggagctaa ggcagtagta gattcaacct cagaaaaagt aggagaatta
    53401 gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg cgttattttg
    53461 tctcaattgt ttcgtggatt ttctaagcaa attccagatg ttgtctcatt aaatgcttct
    53521 gatcttgctg cagccttcac tcatggagtt gaaactgcgt ataaagcagt aatgaaacca
    53581 gtagaaggaa cgattttgac agtagctaga gaagcagcga aagctggaga aaagaaagca
    53641 aaaagtactg atgatgtcgt tgaagtaatg acagctgttg taaaaggagc aaagcgtgct
    53701 ttagctaaaa ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga
    53761 gggcaaggac tgctgtttgt ttatgagggc tttttaagtg cattgaatgg agaattccaa
    53821 gcggatgaaa catatgaacc ttctccagca gaaatggatg atatggtcaa tgcagaacat
    53881 catcgcagtg tccaaggtca aatggcgaca gaagatatcc attttggtta ctgtacagaa
    53941 atcatggtaa aaatcggaga aggacctaca gtcgatagta cattcgatta cgaagaattc
    54001 cgtaactatt tagatggtat aggagattct ttacttgtgg tcaatgatga cgagatcatc
    54061 aaagtccacg tgcatacaga aaacccagga gaagtcttga attatggaca aaaattcggt
    54121 tcattagtca aaatcaaagt cgacaacatg cgcttgcagc atgagacgat tttggaacac
    54181 gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta
    54241 gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt
    54301 atcagtggtg gacaaacaat gaatccaagt acggaagaca ttgttaaagc gattcaagaa
    54361 gtaaacgcag ataaagtgat tgtcttgcca aacaataaga acatcttcat ggcagctgat
    54421 caagcagcag aagtagcaga tatcccagta gcagttgttc cttcaagaac gatttcccaa
    54481 ggattgacag ctatgctttc attcaatgat caagcttctt tagaagaaaa caaagaaacg
    54541 atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc
    54601 tcaattgacg gtgtagagat caaagaagga gactttttgg gcatgatcga tggaaaaatc
    54661 ctgatctcta acccagatat cacagcaaca gcgttaagta cactagaaca aatgatcaat
    54721 gaggacacag agatcgtaac gatcttggca ggtgatgaag gaacagttga acaagcaaat
    54781 gaatttgctg agaaactaac agaaaaacat cctgatttgg aaatcgagat ccaccaagga
    54841 gatcagcctg tctatcctta tctattttct gctgaataat ttctgtgtca gaatatcttt
    54901 ctaacacagt attcattcat aaaaggcctt cggttagtga tcaggtatga tcgctgacca
    54961 agtccttttt ttgttaaagg agggaggaaa aatttatgcg ttctttagat gatccaatca
    55021 ctatgctggc aggagtcgga gaaaaaagag cagctagtct tgccagcttg ggcatccaaa
    55081 cgattgaaga cttactgact tattatcctt ttcgctacga agatattcag gaaagaaatc
    55141 taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg
    55201 tgatgaatcg atttggttat aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg
    55261 atgtgttcaa cgtttctttt tttaatcaac cttatctaaa agaaaaagtc attttatcgg
    55321 aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa agccttgaac gggatgaaga
    55381 ttttaggttc tcaaacaatg gatgactttt cgccgatata tcacgtcaac aaatcaattc
    55441 gtcaaacgac attagttgat ctaatcaaaa aagggtttca agaatacggc acattgattg
    55501 aagaaaatct gccaaacaac cttgtggaga aatatcgact gcttgaccga gcttctgctg
    55561 taagaagtat gcattttcca aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg
    55621 tttttgaaga gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag
    55681 cagaaacgaa tggtttggag atcctttatg atgtccaaaa attgaaacaa tttactcaaa
    55741 agctaccttt tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc
    55801 ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag
    55861 tggttgccgc gattgcgtta tacgctaccg ttacagcagg tttccaaggt gcgttgatgg
    55921 tgcctacaga aatcttggca caacaacata tggaaagctt gcaacagctg tttgatccca
    55981 atgaagtaac catcgcattg attacggggt caacgaaagc aaaagaacga cgtgagctcc
    56041 ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg cacacatgca ttgatccaag
    56101 atggagtcga atttcagaat ttaggtctag ttattacgga tgagcagcac cgttttggcg
    56161 tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc agatgtctta tttatgacag
    56221 cgactccgat tcctagaaca ttagctatta cagcatatgg tgagatggat gtatctatca
    56281 ttgatgaact tccagctgga cgtatcccga tcgaaacacg ctgggtaaga acacctcaat
    56341 tggacagtgt actggattgg acgaaaaaag aactggcaag aggacatcaa atgtatgtca
    56401 tctgtccgct gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg
    56461 aaaaacttcg tgaactattt gcaccaaaat atgaagttgg cttgttacat ggtaaaatga
    56521 agaatcagga aaaagaagta atcatggaag ccttcaaaga caaccagatg cagatattag
    56581 tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca
    56641 tggatgcgga tcgatttgga ttggcacagc tgcaccaact tcgtggacgt gttggacgcg
    56701 gaagtgatgc ttcctactgt atcttagttg ctaatcctaa aaatgaacta ggtgtggaac
    56761 ggatgaaaat catgacagag accaccaatg gttttgtact aagcgaaaaa gatctggaat
    56821 tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg
    56881 ctgacttggt gacggatgga aatgttttgg aggtagccag agaagaggct gcagcccttt
    56941 ggcaaataaa ggattggcag atgcttcctg agtatcagcc tttagcgcaa agcctccaaa
    57001 tagaaaatca ggataatcgt tatttcgatt agcagactgt gctatacttt atactgatga
    57061 cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc aatgggcgga gatcacgcac
    57121 ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga ttttccagaa gtagaattct
    57181 tactttacgg aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa aacatcacga
    57241 ttatccatac agacgaaaaa atcaatagtg atgatgagcc agtaaaagca attcgacgaa
    57301 aaaagactgc atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg
    57361 tattttcagc agggaataca ggcgcgctac tagcagcagg gttgttcatc gtaggacgta
    57421 tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg
    57481 ccggtttcga tatgctggat cttggcgcta acgcagaaaa caaaccagaa catttgttgc
    57541 aatacggtat tttaggttct ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg
    57601 tcgcactctt aaacaacgga acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct
    57661 atgaactcct tcagaatgaa acaagcttga attttattgg aaacgtagaa gctcgtgagc
    57721 tactcaatgg tgtggctgat gtagtggtca cagatggatt tacaggcaac gctgttttga
    57781 aatctattga aggaacagca atgaatatga tgaatctatt aaaatctgct attctaaatg
    57841 aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag
    57901 cagaaatgga ttattccaaa catggcggtg ccgttttgtt tggcttgaaa gcaccagtca
    57961 ttaaaactca tggagcaaca ggccccgatg ctgttcgcta tacgatccgc caaatccaca
    58021 caatgcttga gacagatgta gttggacaat tagtcgaaca atttgaaaaa agcgaagaat
    58081 agtatttgga tatctgaagc gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa
    58141 tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa
    58201 aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag
    58261 atagataccg ataaagtgac ggatgaattg aacatcaaag atgatttaaa agctgattca
    58321 attagtatta tggaattcgt tttagagctt gaagatgaat tcggtacgga aatttcagat
    58381 gaagacgctg aacaaattga aacagtcggt ggcgcagtag attatatcag cagccatctt
    58441 aaataatttc aaataaaagc aaagaaggat aaaaagctgc atgcagcttt ttatccttct
    58501 ttttttaatc tttttgacac gtaaaagtca tttttctgat aattctggcg ataaacagaa
    58561 attatgttgc aaagtaaagc ggttttctat ataattatta ataattcatt acacaaagat
    58621 aagaataacc tttgttataa aacgatttat tctgtgtaat gtcctttttt attaagaaaa
    58681 acttccattt aggaagcaga gaggggtatt cgattgtccg ataaccaatt attagaagtt
    58741 cagaacctac atacaggatt ccgactcaaa gatgactatt acgatgcagt agatgacgtt
    58801 tcatttactt tagacaaaaa cgagatttta gcaatcgttg gggaatctgg gtgcgggaaa
    58861 agtaccttgg ctactacgat tatgggatta cttgatccta ataacacaaa aattacaggt
    58921 gagattttat ataacgatct gaatttaatc gacctgaacg aaacgctcta taataaaatc
    58981 cgcggaaatg atatcggaat gattttccaa gatccgttgt ctgcattaaa tcctctcatg
    59041 agaatcgaag accagatcaa agaaggactt tcctatcaca caaagatgaa tgcagaacag
    59101 cgtcaagctc gtgcactaga attgctagaa caagtaggga ttcctaatcc tgcgagagtt
    59161 ggacgccaat atccccatga actatcaggc ggtatgagac aacgtgtcat catcgcaatc
    59221 gcaatcgcct gcaaacctcc aatcgtcatt gcagatgaac cgacgacagc cctagatgta
    59281 accatccaag cacagattct tgatttactg aaagaccttc aggaagaaac aaaaacaggg
    59341 atcatcttga tcactcatga cttaggagtc gtagcagaaa tggctgatcg agtggcagtg
    59401 atgtatggcg gacagttcgt tgaagtagca ggtgtaaaag aactgtttga tcatcctaag
    59461 catccttata cacgctcttt actgaactct ataccacaag aaggaaatca tgatgcagaa
    59521 ctccatgtga tcgaaggtgt cgttccttct ctgaaaaaca tgcctcgtaa aggttgtcgg
    59581 tttgcaccac gtattccatg gataccagca agcgatcatg aagaagagcc tatccttcat
    59641 gaagtcgcac cgaaccactt ggtacgttgc tcctgctata agcatttcca ctttagagac
    59701 gaaaaaggag aggtgtagat gacagaatta atcacaatca aagacctgaa agttcattac
    59761 cctattcgca gtggattctt caatcgagta acggatcatg tatatgctgt agatggtgtg
    59821 gattttatta tcgaaaaagg aaagacatat ggtttagtgg gagagtctgg ctctggaaaa
    59881 tctactacgg gaaaagccgt tgtgggatta gaaaaggtaa cagccggaca aatcatgtat
    59941 gaaggaaaag atgtaacgaa gcgaagcaac cgcaaaaaaa tgggttataa caaggatgtg
    60001 cagatgatct tccaagattc aatgtccagt ttgaatccta aaaagcgagt gttggatatc
    60061 attgctgaac ccatccgaaa ttttgaacgg ttaagcgatc aagaagaaaa gaaaaaggta
    60121 aaaggactgc tagatatcgt tgggatgccg gaagatgcgc tatataaata tcctcatgaa
    60181 ttttcaggcg gacaacgtca acgtctagga gtagcccgtg ctgttgctac caatccaaaa
    60241 ctgatcgtag ccgatgaacc tgtatctgct ctggatctat ctgttcaagc gcaagtattg
    60301 aatttcatga aacgaatcca gcaagaatac ggcctcagct atttattcat ttcacatgat
    60361 ttaggtgtag taaaacacat gtgcgacaat atcgcaatta tgtacaaagg tcgatttgta
    60421 gaaatcggta caagagaaga catttataat gacccgcgac atatctatac gaaacgtctg
    60481 ctttcagcta ttccgcaaat cgatgtagaa aatagggaaa gccacaaaga aaatcgacga
    60541 agagtagaac aagagtatat ccaaaaccaa aaagaatact atgatgcgac tggtcgagtc
    60601 tacgatttgc gtacgatcac aactacacac aaagtggcgt tgaaagatgg aggtgcaagc
    60661 taatgtggaa aacgattctg cgacgtgttt tattgatgat cccgcaagtt attattttaa
    60721 gtgttctgat cttcttatta gcccaagcga tgccggggga tccatttacc ggtttgatca
    60781 atccaaatca ggatccggca gtgatcgaac agatgcgaca agcagctggc ttgaatgacc
    60841 catggtatgt ccagtacttc cgttggatcg gaaatgcttt gcaaggcgat tttggtcaaa
    60901 gtttcttata caaacgctct gttgctgact tgatcggcga acggatcatc aacactttat
    60961 atctatcgat cttgacagtt gtccttactt atttgattgc tgtgcctctg gggatgcttg
    61021 ctggccggta tcaaaattca atcttggata agattgtcgt aatctacaac ttctttagtt
    61081 ttgctgttcc tttatttatc ttcggcttga tcatgctatt tgtttttggt tatcgtttag
    61141 gctggttccc aacaagtgga tcacaaacac taggtttgca aggtggattt atcactcaat
    61201 ggatagaccg tttgaaatat attctgttac catcaattac ccaagcattt ttggcgactg
    61261 ccgtcacaat ccaatatctg cgaagtgaag taatcgattc gaaatcgctc gattttgtca
    61321 gaacagcccg ttccaaaggg gtaccgacga ataaagtatt cagtcgacat attttccgaa
    61381 atgcagcgtt gccgatggct tctcaaatgg ggtatgaaat cacttcgttg attgctggtt
    61441 ctgtagttat cgaaaagatt tttggttatc cgggagtcgg aaaactattt attgattcca
    61501 tcggccaacg ggactatacc gtgatcacat ccttagtatt gattttagga atcgcgacgc
    61561 tagtggggac attactttca gatatcatca tgagtatcgt tgacccgcgt attcggattc
    61621 aataaaacca atcagaaagg agaatggatc gatgagtgaa aaaaacaata cagcagttca
    61681 agaaagtatt cctcctatgg ggatacgaat gattgctcga gaatttaaaa aagataaaat
    61741 tgctattttt tcattaggtt tgctagtggt tttattatta gtgatcttta tcggtgcgat
    61801 gctgaccgat caagacaaag tgatgtatgt tagtatcttc gacaaatacg ctgcaccagg
    61861 aacagtttcg acacaaggac aaaagttcat tctgggagca gatgaaggcg gacgtgatgt
    61921 attaggacaa ttgatcatag gtgctagaaa ctctgttacg atcggatttg cgatcacgat
    61981 ccttacttcg atcatcgggg ttggattagg gatcatctct ggctactacg gagggatcat
    62041 tgacaacatc ttgatgcgga tcgttgattt tatcatgatt ctaccgatca tgctgatcat
    62101 tatcgtcttc gtatcgatta tttccagata caatgtctgg tcctttatat tcatcatgag
    62161 tgcattttat tgggtagcta aagcccgatt attccgaagc aagacattat ctgaagcaag
    62221 aagagattat gtcagtgcct caaaaacaat gggaaccaat gattttaaaa tcatgttccg
    62281 cgaaatcatg ccgaatctaa gttcattaat cattactaat ttgacgatga actttgcggc
    62341 aaacatcggt atcgagacaa ctttgacttt cttaggtttc ggcttgccgg caaatgtacc
    62401 tagtttaggt acattgatcg gttatgcaag taacggagaa gtcttagtga atcggcaatg
    62461 gatctggtta cccgcatcaa tccttatttt agttttgatg ttgagtatca attatgttgg
    62521 acaagcattc aagcgttctg cagatgcgcg ccaacggtta ggctagtata gaagcgaagg
    62581 gggaaatagg aatgaagaaa acgatttttg gatttgcagc gctgatgtct gctgtcgttc
    62641 ttgcagcatg cgggggcgga ggtacagata cctctggaaa caactctgga ggcaacggca
    62701 ataacaaaga ggaattaact tttccaattg caacaaaaaa taatgatgat gcaatcgaag
    62761 acggcgaatt agaagctgcg gtcgcaacgg acagtcagtt taaaggattg ttccagtggg
    62821 agttttacca agattcatat gatgcagctt ttatggctcc ttctcatgaa ccgctatttg
    62881 caaacgatga cgatttcaga atcacagacg acggagctgc aacgcttgat ctagatgaag
    62941 atacgaaaaa agcgacaatc aaagtaaaag acaacgtcaa atggtcagat ggtgaagatg
    63001 taacggcgga cgatgtgatt ttcccttacg aaatcattgg gaataaagat tacactggta
    63061 tccgttatga cgacactttc cgtaacatcg taggtatgga agaatatcat gatggaagtg
    63121 ccgacacaat ctccggaatc aaaaaaatag acgacaaaac agttgaaatc gaatataaag
    63181 aagtaaaccc aagcatgctt caatcgggtg gtggtatctg gtcatatgca gcaccaaaac
    63241 atacgttaga aggtgtagca atcaaagata tggaatcaag cgatcaagta cgtaaaaatc
    63301 cagtgacttt tggcccttat tacatgagta atatcgttgc aggagaatca gtagaatact
    63361 tgccaaacga gcactactgg aatggtacgc ctaaattgaa aaagatcacg atgaaatctg
    63421 taccgatggc aagtgcaact gaagcattga actctaaatt atatgatatg attttccaaa
    63481 tgccaacaga tacttacgat acctataaag aaattgctgg ctatacaaac ttaggccgtg
    63541 aacaaacttc ttatacttat ctaggattca agttaggtaa atggaatgca gatacaaaga
    63601 gtgtggaata caatccagat tcaaaaatgg ctgataaatc attacgtcaa gcaatgggct
    63661 atgcattaga taataatgca gtaggtgaac gtttctatgc aggtttgaga agcaatgcga
    63721 cttcattgat tcctccagta ttccagtcat accatgatga tgagatgaaa ggattcacac
    63781 aagatctaga caaagcgaaa aaactattag atgatgctgg ctacaaagat gtggatgacg
    63841 atggtttaag agaagacaaa gatggaaata aactaacaat caactttgct tcgatgtccg
    63901 gtggggaaac agcgcaacca ttggcagatt actatatcca acaatggaaa gaaattggtt
    63961 tggatgtcca atacacaaca ggtcgtctga tcgaattcaa ttctttctat gataaattag
    64021 aaaacgatga tcctgaaatt gatgtttacc aagctgcttg gaatacagga actgatccaa
    64081 atccaagtgg actatgggga ccaaatgctg cgttcaacta cactcgttat gaatcagaag
    64141 aaaatacaaa attgatcaac gatatcagtt caagcaaagc atttgatgaa gagtaccaaa
    64201 aagaagcata tcaaaaatgg caacaatatg catttgaaga agcatttgcg attccaacgt
    64261 tgttccgtaa tgaagtattg ccagtcaacg accgtgtcaa agactgggat tggtcttatg
    64321 atgtaccaaa cccatgggca aatgtttctg ttacagcaga ttcacgttca taatcactaa
    64381 aaatgaccga aaaagggcga tgtccttttt cggttttttg atatttttct gtttatttct
    64441 ctaatattca ggtcaaggcg ctgatttcat gacttctcca ataaacggtg ttcaaagtcc
    64501 agtttctcgg tattaagtca aatttatgca aaaatatgga aagccatttt cacaaaattt
    64561 tttcttcata ctcgaaacta aacgaatttt tcacaacttc tcgtcaatca ctaggattat
    64621 taaagcttta cttacctttt gaccgaacca tttcaaattt gacaaaaaac cagtataata
    64681 aggaagtatc gaaaaaatat ccataaaagg aatgtggaaa atggacaacc agctgacaaa
    64741 agaactaaaa gaaaactata acatcgtttt tcacgatcta aatttgcttg aacaagcttt
    64801 tacccattca tcctatgtga atgagcatcg caatctgcaa ttatcggata atgagcgact
    64861 tgaattttta ggagatgccg ttttagaact gatggtttct cagtatttat tccgtttata
    64921 tcctcatttg ccagaaggaa aattgacaaa aacacgagct gcgatcgttc gcgaagacag
    64981 cttgtcgaaa tttgccaaag aatgtcactt tgatcaatat gttttgttgg gcaagggaga
    65041 agagaattca ggaggacgta cgcgaccagc attgctttgc gacctattcg aagctttttt
    65101 aggcgcgtta tatcttgacc agggattcga cacgacccat gcattcatcg aaaaagtgat
    65161 ttttcctaaa gtcaaagcag gtgctttttc acatgagatg gatcataaaa caaaactaca
    65221 agaagtcttg caaaaatcag gtgatgtatc aatcgaatac cgattgatca atgaagaagg
    65281 tccagctcat gaacgagtat tttggatcga agtttatgta gatgatcaat tgattggcac
    65341 tggacaaggg aaatcgaaaa aattagcaga acaagctgcg gcggaaaatg cattggcagc
    65401 actgtaaaag taagtgaggg aaagctttgt atttgaaacg aattgaaata gccggtttta
    65461 aatcatttgc agacaagaca gtcattgatt ttgaaaatag cgtcacagca gtggttggac
    65521 caaatggcag cggaaaaagc aatattacag aggctatccg ctgggtatta ggagaacaat
    65581 ctgctaaaag tctgcgaggc ggaaaaatgc cggatatcat ctttgccggt tccgataccc
    65641 gtaagcagct gaatattgca gaagtcacag tgattttaga caatagtgat cattatctgc
    65701 cattggagta caatgagatc agtgtaaccc gccgttatcg acggactggg gaaagcgagt
    65761 ttttcatcaa taaacaaagc tgcagattaa aagatatcca agaattgttc ttagattctg
    65821 gattaggaaa agaatcgttc tcaatcattt ctcaaggaaa agtagaagcg attttcagta
    65881 gtaaaccgga agaccgtcgt gggatctttg aagaagcagc tggtgtcttg aaatacaagc
    65941 agcggaaaaa gaaagcagaa cagaagcttt ttgaaacaga agacaacctc agccgcgtcc
    66001 aagacatcat ccacgaattg gaagaacagt taactccttt ggccgcccag agtgaggcag
    66061 caaaggagtt tttacggcta aaagaaacac tgactcaaac cgatgtctct ttgatggttg
    66121 ctgaaatcaa gacagcaaaa aaagattggg acaacaaaca agcacagctt gcgaaattca
    66181 atctagaatt aggcaaacta tctgagtcga tccaagaaca agaatccatt ttggcgaagc
    66241 agcgtaaaga aaatgcgcaa gccgatcgtc tgatcgaaaa aaatcagcaa gtattattag
    66301 atctttcgga aaaactgaag cagacagaag gacaaaaaga tgttttgcag gagcggacaa
    66361 agcatacaca aaaaagctcg caagaatatc aaacttcatt agcagaagca caaaaaaagg
    66421 ttaaacattt tgaaaagctt caagaaagct tgatgaaagc agcagcagaa aaagaaacag
    66481 agatccaaaa agctgaagca aatctcataa aaacacagca agaattagaa aaataccaaa
    66541 aatcaacgaa agaattattg gcagaattaa gagatcaata tgttgatttg atgcaagaac
    66601 aagcagctgt aggaaacgaa ctgaagtatc tagaacgtca atatatacaa gaaacagcaa
    66661 aaagcaagca gacgttagcc aagcaatcgg aagtagaagc atcagtagac cgattgatct
    66721 tacaaaaaga agaattgaca caaaagcagg cgcagctaaa atcatcttta actgaaacaa
    66781 aagagaagtt agaaatgatc caacaaaacg ggaaaaagtt ccaagaagca ttagcaaaag
    66841 aacagcctaa aatgtatcaa ttaatgaatc aagtccagca gctacgtgct cgtaagaaaa
    66901 gccttcaaga aatccaagaa aactattttg gtttttacca aggggttcga ttggtcttgc
    66961 agcataaaca gcagctttcg ggtattgtcg gagcagtggc tgaactgatc gatgttcctg
    67021 cagatttcac gttagctatc gaaacagctt taggtggggc agcccagcat gtcatcgttg
    67081 aaaatgaaaa agatgctcgg caagccatca cgtatttgaa acagcaaaga ggaggaagag
    67141 caacattcct tccactgacg acaatcaaac caagacaatt accggctcat atattgacac
    67201 aagcagcagc tgtagaaggg tttataggga ttgccagtga acaagtcagt tatcccgatc
    67261 aaatccaaac tgtggttcaa aacctgctag gaacgatcct tttagccaaa gacttaacta
    67321 gtgccaatgc aatcgcgcaa acgatccgct atcaatatcg agtggtttcc ttagaaggtg
    67381 atgtgatgaa tgccggagga tccatgacag gtggagcgaa taaacgagga aaccaaggca
    67441 gtttgtttgt acaaaatcaa gaattgaaac agttgactag tgaatttgaa gaagcagata
    67501 aacaactcca agcacaagaa aaaaaagttc aggagctgca acaagaaaca gctcgtttag
    67561 ccgaagaaca agaagttttg cgtacacgag gcgaacaatt acgttttgaa gaacaagaag
    67621 cgacaaatca gctacaaaac atcataaatg aacttgaacg ttttgaaaaa gaaaagcaaa
    67681 tctcaacttt tgagacacgt gaactgcagc agtttatcga agattacgaa aagcaaacga
    67741 acgaattgaa agacaaacaa acagatcttg aatcgcaacg acaaaaaatc gacgaagaaa
    67801 tcaaatcgtt aagtcaagaa agcgaccaga tggaagccag aagagcacaa gtacaaagtc
    67861 aaaaagcaca agaacaagca gatcttgctg tcttgaaaga gcagtttaat catctgcaga
    67921 tccagctccg cggtgcccga gtccaaaaag cagaggcaac cgaacgtcaa gaagcgatag
    67981 aaaagcagtt ggcgacattg acagcagact tttctgatca tgaagtcaca gaagaaagtt
    68041 tggaaaaaca aattaacgaa ttatctgctc agcgagaaac attaaaagcc gagttggcaa
    68101 aagccaaaga gcagcgagat agaaggcaaa aggagatcga taagcttgaa gcagtcttgg
    68161 cagagcgtaa tcgtgagcaa aaagcacgac tgtcggaaca atccaaatta gaagtccaaa
    68221 aagaccgagc tgaaatgatt ttagataatc atttgaccta tctgcagtca gaatatcagc
    68281 tgacatttga gaaagcttcc caagactatc aagaaacaac cgatatcgaa gacagtagaa
    68341 caaaggtttc atccttaaaa gaacagattg aaaaactggg accagtaaac ttaaattcaa
    68401 tcgaacaata cgaacaagtc agtgaaagac atacattctt agcaacacag cgagatgatc
    68461 tgttagcagc gaaaaaccaa ttgtttgaaa cgatggatga gatggatgat gaagtgcgga
    68521 caagattcaa agaagtattt gaagctatcc gtcaagaatt caaaattgtc tttccaaata
    68581 tgtttggcgg cggtaaagct gaattagtac tgacggaacc aactgatttg ctgaaaacag
    68641 gtattgagat tgaagtacaa cctcctggca aaaaactgca aagtctaagt ttgctttctg
    68701 gaggagaacg agcacttaca gccatcgcgt tgctcttttc cattatccgt gtctgtcctg
    68761 ttccgttttg tatcttagat gaggtagaag cagcgttaga tgaagccaac gtgaaacgtt
    68821 ttggtcgtta cttgagcgat ttccaagatg acacacaatt tatcgtagtc acacaccgca
    68881 aaggaacgat ggtcgcagca gatgtactgt acggagtgac gatgcaggaa tccggtgtat
    68941 caaaaatcgt ttctgtacgg atggaagata tcaatgaaga ggcgaaaatc aacgaaacga
    69001 aagtgtagga acgaaaatga tcaaattagt agcaatcgat ttggacggta cattattaaa
    69061 tgaacaaaag ctgatttcag atgagaataa acaagcatta gcacaagcaa aacagcaagg
    69121 ggtcaagatc gttctatgta caggacgccc cttagctgcg atggcgcatt atctgcaaga
    69181 attaggactg gtagatgaag gtgactacag catcactttc aacggcggtt tggtacaaaa
    69241 aaatgatact ggtgaaatca tggaaaagaa agtgatggaa gtatcagata tccaacgctt
    69301 atacaaactg gctcagcaat tggatctgcc actagatgtt ttgtctgaca atgtggtttt
    69361 acagctgcct agtgccccaa ggaagcaatc cttgtacaat gtgttgaata agctgctgca
    69421 attccaacca gcggatataa gcgatattac agatgaattt gtgcttaata aggcagtaat
    69481 cgcttatgac caagaagaat tagatcctaa aatcaaagaa ataccagcag aattccatga
    69541 aatgtatgag atcatcaaaa caagaagtat gcttttggaa tttatgccaa aaggtgttac
    69601 aaaagcttat ggtatttctt tattggcaaa agatcttggt ttgaaacaag aagaaatcat
    69661 ggcgatcggt gatgaggaaa atgatttacc gatgatccaa tatgctggac taggcgtagc
    69721 gatggagaat gctgtgcctt tcgtcaaaga agcagcagat ttcgtgacag ccagcaatga
    69781 agaaaatggc gtagccaaag tgatccaaaa atttgtatta gagtaggggg agtcaaatgg
    69841 gattttttga taaaatcaag cgtgctttta ctggcgaaga gaaaacagaa gaagttgaag
    69901 tacaggagaa gtacgacaag ggtttagaaa agacgcgtaa aacatttggt gaacggatga
    69961 atgagttgtt tgccaatttt cgaacagtcg atgaagactt ttttgaagaa ttagaagaaa
    70021 cacttatcgg tgcagatgta ggttttgaaa cagcgatacg aattaccgaa gcattacgtc
    70081 aagaagtgaa actcagaaat gcgaaaaaat cggcagaagt ccaaaatgcg atcatcgaga
    70141 aacttgtgga tctctatgaa gcggaaggaa tcaatgaagt aaatgaacta aatatccaag
    70201 aagacggact aacagttatt ttgtttgtcg gtgttaatgg tgtagggaaa acaacaagta
    70261 ttggtaaact tgcccatcag ttcaaaaacg aaggtaaaaa agtattgatg gcagcagcag
    70321 ataccttccg cgctggagca atcgaccagt tagttgtctg gggcgaacgc gctggtgttg
    70381 aagttgttcg tggcaatgca ggaggcgatc cagcagctgt cgtattcgat gctttggaaa
    70441 gagcaaagac agagcaggca gatgttttac tagttgatac tgcaggccga ctgcaaaata
    70501 aagtgaactt gatgaacgaa ttagaaaaaa taaaacgagt aatccagcgg gaattaccag
    70561 aagcacccca agaaacactt cttgtattag atgcaaccac tggtcaaaac gcgatggttc
    70621 aagcaaaaca attcaaagaa acaacagatg tgaccggtct tgttttgaca aaattagatg
    70681 gtacagcaaa gggcggtatc gtacttgcta tccgtaatga attgcattta ccagtgaaat
    70741 tggtcggtct tggtgaagga atcgatgact tggagccgtt tgatccaaat gattttgtcg
    70801 ttggattgtt caaaggatta ctaaaagaag aataacccat gtctaaaaag caggagctgt
    70861 gacaaaagtt gcagctcctt gtttgaatga ttgtttttaa catagctgtg tttttgatgc
    70921 cttcattttt atacttattt gtaagcggtt ctcaaacaat ttcgttcttt ttcttagact
    70981 ttttgattat aattagattc atatgaattg gaggtcactt aatgatgaat tttaagcaat
    71041 tattattgca tgtcaatgcg cgccctttta tcgaccaagc tcgatttggt atagaacggg
    71101 aaggacagcg ggttgatctt gcaggaaatc tagcaaaaac cgatcatcca gcaatctttg
    71161 gcgatcgatc ctatcacccc tatatccaaa cagattttag cgaaacacaa acagagatga
    71221 tcacccctgt taccgattct attcccgaat tatttcagta tctcgctgct gtttatgatg
    71281 tgactgctcg ttctataccg aaagaagaga tgatctggcc attaagtatg ccacctgcct
    71341 taccggaaaa agacgaagag atcattattg caaaattaaa aaatttcgaa gatgtcttgt
    71401 atcgacgtta tttagcaaaa gaatacggga agcgaaaaca gatggtaagc ggtattcatt
    71461 tcaattttga atttggcgat gagttgctaa gaacattgtt cagccatcag gaagaatttc
    71521 aagatttttc cgaattcaaa acagaacttt atttgaaaac agcgagaaac tttatgcgct
    71581 atcgctggat gatcacttat ttattcggtg cttcaccaat gagtgagaag aactactttt
    71641 tagatgaatc acatccgcaa gaacctgttc gcagtattcg caacagtgcg ctgggctata
    71701 cgaatcatcc aaatgtgaaa gtttcctatg cttctatgaa acagtattta gcagatatcg
    71761 agcgaatgat cgaagaaggg aaactttcgg aggagaaaga attttataca ccgcttcgtt
    71821 tccgaggcgg aaagaaagtc gcagatttag caacaacagg tgttcgttat attgaattgc
    71881 ggaatatcga tttgaatcct tatgcaagat tgggtatcaa tccagagcaa gttcggttct
    71941 tacaattatt cctgatgtac atgttatgga cagaagaaaa agaagactgt gaccaatggg
    72001 tagcagaagg tacgactcga aataacaaag tagcactaga acaaccatca gatcaaacag
    72061 aatttcatca agaaggtaga gaaattcttg aaggaatgaa gcaaatgctg gttgaattgg
    72121 attggttgga ttctctttat ttagtagagg aagcattgac tcaaatggat catcctgaac
    72181 aaacgttagc agcgaaactc tatcaagaag cacagctatc gagtcagcaa gaagttgctg
    72241 tggcattggg acatcaatat tataaagaaa gccatgaacg tccctatcag ttggccggtt
    72301 ttcgcgaaat ggaactttct actcagatat ttatgttcga tgccatccaa aaaggtgtcc
    72361 aagtcaaagt attagatgag tcggatcaat ttttgcgtct gcaattccaa gaccatgtag
    72421 aatatgtgaa aaatgcaaac atgacaagta aggacagcta tatcgtccca cttatcatgg
    72481 aaaacaaaac agtaacgaaa aaagtcttaa aagaagctgg gttccgggta ccaggaggcg
    72541 cagaattttc atccatggaa gaagcggtaa aagcttatcc gagatttgcc gagcaggcat
    72601 ttgtgatcaa acctaaatca accaattacg ggttaggtat cacgattttc aaggaaggag
    72661 ctagtctgga agattatcaa gcaggtttag ccattgcttt tcgtgaagac agttcggttt
    72721 tagtagaaga atttatgccg ggaacggaat atcgtttctt tgtgatcgac ggagaagtcc
    72781 aagccatcat gttgcgagtc cctgctaatg ttattggtga cagtatccga acagtgaaag
    72841 aacttgttga agaaaaaaat agtgaccctt tgcgtggaac gaatcatcgt gcacctttag
    72901 aattgatcca attaggtgaa ttagaacaat tgatgttgaa agaacaaggg ttgacgatcg
    72961 aatcagttcc ccaagccaat caaattgttt atctaaggga gaactcaaat atcagtacag
    73021 gtggtgattc gatcgatatg actgatgaat tttctgaagc ttacaaaaaa atcgccgttt
    73081 ctgcggtaga agcactagga gctaagatta gtggtatcga tttgattata ccagataaag
    73141 aaatcgatcc tacaactgat aaaaaagcat atgggatcat cgaagcaaat ttcaatccag
    73201 ccatgcatat gcatgtttat ccttttgcag gaaaaggcag aagattgacg atgaatgtat
    73261 tgaaattgtt atatcctgaa gtattttaaa agaaaaagct tttgacagat tgtcctattt
    73321 gatgtcaatc tgatcaaaag ctttttttgc atgtaaaaat ttatgtgttt tttcgtttta
    73381 aaataaacga tcccagaagc tttttttctt ttttggtttt tctttttcag ctggcgaaga
    73441 gttttcttct gggtattttt tttcgatatc aatcacaggt tcatttactg cttcttttgc
    73501 tgttaagagc aatccgaatt cctcaggatt tgcagtgacg taatcgttga ctacagtgaa
    73561 acggctattg tttttcgttg ctagctggat atattggttt tggacagaaa ggggtagagc
    73621 cccattcaag agtatggaag catctggatg ttttttgagt tcttttaaaa agttttcttt
    73681 gttttttggt tggatcattt cagcaacagt catactaaga tagcagcgtt ccctgaatgt
    73741 ccctaaatat ttatgctgtt cttcagggtt cactaaaggt gtaccataaa gtcccttatc
    73801 caaatggctt tttacatcat cttgtgccat aaaaaatacc ctccttcttc tctttgtctt
    73861 tcttacgatt atagcatatt cccaataata cctaaaacat atgaaaaagc ggttgctttt
    73921 gttcaacgat tcacttaaaa taagaatgaa aatcattctc aattagaaag gtggattgga
    73981 tgcgctacgg acgactagta gcattattag ttattttaag tagtatttca ttgttcatcg
    74041 gagtatcgaa tgtacaattg tcagaactgc tccagttaga tgggcaaagc ttacaaatct
    74101 tgctagtgac tcgtttgcca cgaacgattt gtttgatcct tgtgggagcg accagtagta
    74161 tttgtgggtt gatcatgcaa catttgaccc aaaataaatt tgtttcacca acgactgcgg
    74221 gcacgatgga tagtgcgcgt ttaggtattt tagtggctat gattttccta cctggtgcat
    74281 ccttgattgt ccgatccttg acagcatttt gctttgcttt tgcaggaaca ttgctttttt
    74341 tatcccttac gcgattgttc ctccagaaaa atcaattgat gcttccgttg attggagtca
    74401 tgttagggaa tatcattggt tcagttgcta ctttttttgc ttatcaattc cagttggtac
    74461 aaaatatgac ttcatggctt caagggaatt ttgcgactgt gatacgagga aattatgagt
    74521 tattgtatgc tgttgttcca ttgttgctct tggtttggct atttgcttac cgatttacga
    74581 tcattggtct gggcgaatcg tttgcagtaa atctgggaat agattaccag aagatgcagt
    74641 ttttaggcat tggcttagta tcattggcta gtgcagtcat gttgatcatg gtaggaagca
    74701 ttccttttat cggagtgatc attccgaatc ttgtctctag aatatatgga gatcaagtgc
    74761 acaaaacaat tggcgtaact gctttagcag gtagtttgtt cttagtattt tgcgatcttg
    74821 tcgctcgcgt gttgattttt ccttatgaaa taccagtcag tgtgatcgtt ggaatcatcg
    74881 gtggggctat cttcctttat cttctgataa ggggacgacg atatgcttaa aaaaaagcca
    74941 tctattttta ttggtatgct tgttcttgtt ttatttgtta caggaatgta cttgacgatc
    75001 catacatatg gaaactggca ttttgcatgt gcattgagga gtaagaaaat ttttgcattt
    75061 atccttgtct cgctagctgt cagcagtgcc acaatcagct ttcagacaat tacgcataac
    75121 cagtttttga ctccttcagt attgggactt gagtcgttat atgtccttgt ccaaacggtc
    75181 cttttcttct ttatcggagg agtgacgatg cttcagcaga caggtacagt ttttttctta
    75241 ttgaacgttg ggctgatggt cgggctgagt ctattgttat ttttctttct tttgaaaaaa
    75301 atgagagcga atcttttttt attactgatg tcagggatga ttttaggtac gctttttggt
    75361 agtgtcagta cctttttaca agtcatcatg gatccgaatg aatatgattt gcttcaaggc
    75421 agattatttg caagctttgg caatatccag agtcagtatc ttttgcctgc ttctttgatc
    75481 attatcggct gctggtacct gttatttcga tatcgacatg tgctagacgt tttccatctc
    75541 ggaaatgaac aagcagtaaa cctcgggata gacacagatc gttttgaatt tttgatttta
    75601 tgttgtgtca gtcttttgac agggacatca acggctcttg tgggtccagt tactttttta
    75661 gggtttattg tcgcaaatat cagttatcgt gtcttttcta cttatcgtca tggtcagcta
    75721 tttatcggaa gctttctggt agctatgctg tttctgacag gaggacagct cgcagtagag
    75781 cagattttcg gctggaatac aacagtgagt gtcgtcgttg aatttgtcgg aggaatttat
    75841 tttgtcacaa aattgattcg cgaaaggaag agtatcctat gattgattta caaaaggtca
    75901 ataaactgta ccatggaaaa tcagtggtta aagatgtaca gttaacgatt cctgaaaaga
    75961 aattgactgc ttttatcggt ccaaatggtg ctggaaagtc aacggtgttg tcgatgatca
    76021 gtcgtttgat accaaaagat acaggtgaaa tctatttaga ccataacgaa gtaaaggctt
    76081 ggaaatccaa tgaattagcc aaaaaattat cgatcctacg acaatcgaat gaaataaatg
    76141 tccaaatcac cgtgaaagaa ttagtagaat ttggacgatt tccttatacg aaaggaaaat
    76201 tgacgacaaa agacgaggaa atcgttcagg aatcactgga acaccttggg ttagaagaaa
    76261 tagcggatca atcgattgac actctttcag gcggtcaact ccaacgtgcg tatattgcaa
    76321 tggtgctagc gcaagacacg gattatattt tactggatga acctttgaac aatctagata
    76381 tgaattttgc ggttcaaatc atgcagattt taaaaaattt agtacaagaa atcgggaaaa
    76441 caattgttat tgttcttcat gatatcaact ttgctgcaag ttatgcagac gaaattgttg
    76501 caatgaaaga tggacagtta tttacgcaag gaacgaccca agaaatgata aaaaaagatg
    76561 tattggatga attatacgaa atggacatac ggatttgtga gattgaaggc cgcagattct
    76621 gtctttattt ctaaaactaa cattaggaga gaagagatga aaaaattagt agtaggtatc
    76681 ttgattctag catcatttgg tttagcagca tgtggaaata gcaacaacac cagtcaggca
    76741 gacacaaaaa aaagcagcac acaaactgaa actacattga cgataacaga tagtaatgga
    76801 gaccaaatcg aggtgccaaa taatccagaa aaagtcgtcg tattcgacaa tggttcttta
    76861 gatacgatgg atgctcttgg tgttggagat aaggtagttg gagcagctac aagcagcctt
    76921 cctgagtatc tttcatccta taaaaaagta gaatcagcag gtggaataaa agaaccagac
    76981 ttagaaaaaa ttaatcagct tcagccagat ctaatcatca tttctggccg acaaagagat
    77041 tttcaaaaag atctaagtca aattgctccg accattttcc tttctttaga tgcgaaaaat
    77101 ccatgggaat ctttccagca aaatgtcact gctttagggg agatctttgg taagcaagaa
    77161 gaagctaaaa ctcaattaga ggaattatct agtgcaatcg atcagacgaa aaagaaagca
    77221 gaagctactg acaaaaaagc actagtcaca cttgtgaatg aagggcaatt atcagcctac
    77281 ggatctggtt ctagattcgg tttcatccat gatttatttg gctttgaaca agcagatgat
    77341 caaatcgaag catccactca cggtcaaagt gtttcttatg aatatgtgct ggaaaaaaat
    77401 ccggatatct tatttgtagt agacagaaca aaagctattg gtggagacga cagcaaagat
    77461 gatatttctg ccaatgaact ggtagcacaa acgaatgctg gtaaaaatca gcagatcatc
    77521 tcccttgaac cagatgtctg gtatttaagc gggggcggtt tagaatcaat gaaactgatg
    77581 atcgaagatg tgaatcaagc atttaaatag cataaataac agaggattaa actctcatac
    77641 tatgtaacaa taatgtttct tacattctta aatagcaaga gcctatcctc aaagaagtta
    77701 tcaaaaacca tccgtaaaat cgtattgatg attttacgga tggtttttat ttatttgaga
    77761 aatctttttt caacgtgaat tcattatcta catcatacgg gttgattcta gaatggggag
    77821 tggcaaaaaa tagtgtgatc cattttttca gcatcaagat cgcctccttt gatttgaatc
    77881 aaaggtacaa agcgaaactt agataaaact taaatttaga tatttttatt ttgtaccgat
    77941 ctcttcttta aacgatgttc catctgctgc ttctcggcaa taag
//
LOCUS       DACGLH010000007        71797 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.008, whole genome shotgun sequence.
ACCESSION   DACGLH010000007 DACGLH010000000
VERSION     DACGLH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.008"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(55..1068)
                     /locus_tag="IXT21_000495"
     CDS             complement(55..1068)
                     /locus_tag="IXT21_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4867929.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1146..1334)
                     /locus_tag="IXT21_000496"
     CDS             complement(1146..1334)
                     /locus_tag="IXT21_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ4867930.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(1774..1902)
                     /locus_tag="IXT21_000497"
     CDS             complement(1774..1902)
                     /locus_tag="IXT21_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ4867931.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(1905..3278)
                     /gene="rlmD"
                     /locus_tag="IXT21_000498"
     CDS             complement(1905..3278)
                     /gene="rlmD"
                     /locus_tag="IXT21_000498"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ4867932.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(3389..4444)
                     /locus_tag="IXT21_000499"
     CDS             complement(3389..4444)
                     /locus_tag="IXT21_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ4867933.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(4454..5884)
                     /gene="gatB"
                     /locus_tag="IXT21_000500"
     CDS             complement(4454..5884)
                     /gene="gatB"
                     /locus_tag="IXT21_000500"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ4867934.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(5881..7353)
                     /gene="gatA"
                     /locus_tag="IXT21_000501"
     CDS             complement(5881..7353)
                     /gene="gatA"
                     /locus_tag="IXT21_000501"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ4867935.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(7353..7658)
                     /gene="gatC"
                     /locus_tag="IXT21_000502"
     CDS             complement(7353..7658)
                     /gene="gatC"
                     /locus_tag="IXT21_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ4867936.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(7764..9800)
                     /gene="ligA"
                     /locus_tag="IXT21_000503"
     CDS             complement(7764..9800)
                     /gene="ligA"
                     /locus_tag="IXT21_000503"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ4867937.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(9817..12051)
                     /gene="pcrA"
                     /locus_tag="IXT21_000504"
     CDS             complement(9817..12051)
                     /gene="pcrA"
                     /locus_tag="IXT21_000504"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ4867938.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            12206..13765
                     /locus_tag="IXT21_000505"
     CDS             12206..13765
                     /locus_tag="IXT21_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ4867939.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(13876..15807)
                     /locus_tag="IXT21_000506"
     CDS             complement(13876..15807)
                     /locus_tag="IXT21_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4867940.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(15825..16739)
                     /gene="pfkB"
                     /locus_tag="IXT21_000507"
     CDS             complement(15825..16739)
                     /gene="pfkB"
                     /locus_tag="IXT21_000507"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ4867941.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(16736..17488)
                     /locus_tag="IXT21_000508"
     CDS             complement(16736..17488)
                     /locus_tag="IXT21_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4867942.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(17648..17857)
                     /locus_tag="IXT21_000509"
     CDS             complement(17648..17857)
                     /locus_tag="IXT21_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867943.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(17868..19196)
                     /locus_tag="IXT21_000510"
     CDS             complement(17868..19196)
                     /locus_tag="IXT21_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867944.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYQDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(19561..20148)
                     /locus_tag="IXT21_000511"
     CDS             complement(19561..20148)
                     /locus_tag="IXT21_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ4867945.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(20231..21481)
                     /gene="clpX"
                     /locus_tag="IXT21_000512"
     CDS             complement(20231..21481)
                     /gene="clpX"
                     /locus_tag="IXT21_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ4867946.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(21728..23017)
                     /locus_tag="IXT21_000513"
     CDS             complement(21728..23017)
                     /locus_tag="IXT21_000513"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ4867947.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(23171..24103)
                     /locus_tag="IXT21_000514"
     CDS             complement(23171..24103)
                     /locus_tag="IXT21_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867948.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(24260..24919)
                     /locus_tag="IXT21_000515"
     CDS             complement(24260..24919)
                     /locus_tag="IXT21_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ4867949.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(24922..25947)
                     /gene="sppA"
                     /locus_tag="IXT21_000516"
     CDS             complement(24922..25947)
                     /gene="sppA"
                     /locus_tag="IXT21_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ4867950.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(25960..26250)
                     /locus_tag="IXT21_000517"
     CDS             complement(25960..26250)
                     /locus_tag="IXT21_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867951.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            26659..28464
                     /gene="glmS"
                     /locus_tag="IXT21_000518"
     CDS             26659..28464
                     /gene="glmS"
                     /locus_tag="IXT21_000518"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ4867952.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(28536..29891)
                     /locus_tag="IXT21_000519"
     CDS             complement(28536..29891)
                     /locus_tag="IXT21_000519"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ4867953.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(29915..31084)
                     /locus_tag="IXT21_000520"
     CDS             complement(29915..31084)
                     /locus_tag="IXT21_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867954.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(31081..31968)
                     /locus_tag="IXT21_000521"
     CDS             complement(31081..31968)
                     /locus_tag="IXT21_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ4867955.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(32157..35837)
                     /gene="nifJ"
                     /locus_tag="IXT21_000522"
     CDS             complement(32157..35837)
                     /gene="nifJ"
                     /locus_tag="IXT21_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ4867956.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELQKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHAPAYLHSYNLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVIPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYVQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            36035..36556
                     /locus_tag="IXT21_000523"
     CDS             36035..36556
                     /locus_tag="IXT21_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ4867957.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(37255..37820)
                     /locus_tag="IXT21_000524"
                     /pseudo
     CDS             complement(37255..37820)
                     /locus_tag="IXT21_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37999..38655)
                     /locus_tag="IXT21_000525"
     CDS             complement(37999..38655)
                     /locus_tag="IXT21_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867958.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(39223..40158)
                     /gene="whiA"
                     /locus_tag="IXT21_000526"
     CDS             complement(39223..40158)
                     /gene="whiA"
                     /locus_tag="IXT21_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ4867959.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(40192..41190)
                     /locus_tag="IXT21_000527"
     CDS             complement(40192..41190)
                     /locus_tag="IXT21_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ4867960.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(41187..42071)
                     /gene="rapZ"
                     /locus_tag="IXT21_000528"
     CDS             complement(41187..42071)
                     /gene="rapZ"
                     /locus_tag="IXT21_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ4867961.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(42206..42937)
                     /locus_tag="IXT21_000529"
     CDS             complement(42206..42937)
                     /locus_tag="IXT21_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ4867962.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(42941..44206)
                     /locus_tag="IXT21_000530"
     CDS             complement(42941..44206)
                     /locus_tag="IXT21_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ4867963.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(44469..47285)
                     /gene="uvrA"
                     /locus_tag="IXT21_000531"
     CDS             complement(44469..47285)
                     /gene="uvrA"
                     /locus_tag="IXT21_000531"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ4867964.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(47295..49289)
                     /gene="uvrB"
                     /locus_tag="IXT21_000532"
     CDS             complement(47295..49289)
                     /gene="uvrB"
                     /locus_tag="IXT21_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ4867965.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            49548..51050
                     /locus_tag="IXT21_000533"
     CDS             49548..51050
                     /locus_tag="IXT21_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4867966.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            51052..51789
                     /locus_tag="IXT21_000534"
     CDS             51052..51789
                     /locus_tag="IXT21_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867967.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(51834..52007)
                     /locus_tag="IXT21_000535"
     CDS             complement(51834..52007)
                     /locus_tag="IXT21_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ4867968.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            51983..52648
                     /locus_tag="IXT21_000536"
     CDS             51983..52648
                     /locus_tag="IXT21_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ4867969.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(52730..53896)
                     /gene="dnaJ"
                     /locus_tag="IXT21_000537"
     CDS             complement(52730..53896)
                     /gene="dnaJ"
                     /locus_tag="IXT21_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ4867970.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(54047..55876)
                     /gene="dnaK"
                     /locus_tag="IXT21_000538"
     CDS             complement(54047..55876)
                     /gene="dnaK"
                     /locus_tag="IXT21_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ4867971.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(55927..56490)
                     /gene="grpE"
                     /locus_tag="IXT21_000539"
     CDS             complement(55927..56490)
                     /gene="grpE"
                     /locus_tag="IXT21_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ4867972.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(56584..57627)
                     /gene="hrcA"
                     /locus_tag="IXT21_000540"
     CDS             complement(56584..57627)
                     /gene="hrcA"
                     /locus_tag="IXT21_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ4867973.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(57861..59027)
                     /locus_tag="IXT21_000541"
     CDS             complement(57861..59027)
                     /locus_tag="IXT21_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ4867974.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(59043..59270)
                     /locus_tag="IXT21_000542"
     CDS             complement(59043..59270)
                     /locus_tag="IXT21_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ4867975.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            59754..60344
                     /locus_tag="IXT21_000543"
     CDS             59754..60344
                     /locus_tag="IXT21_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ4867976.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            60534..61247
                     /locus_tag="IXT21_000544"
     CDS             60534..61247
                     /locus_tag="IXT21_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867977.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(61362..62372)
                     /locus_tag="IXT21_000545"
     CDS             complement(61362..62372)
                     /locus_tag="IXT21_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ4867978.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(62409..62891)
                     /locus_tag="IXT21_000546"
     CDS             complement(62409..62891)
                     /locus_tag="IXT21_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ4867979.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            63041..63409
                     /locus_tag="IXT21_000547"
     CDS             63041..63409
                     /locus_tag="IXT21_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ4867980.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            63874..64326
                     /locus_tag="IXT21_000548"
     CDS             63874..64326
                     /locus_tag="IXT21_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4867981.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(64450..65301)
                     /locus_tag="IXT21_000549"
     CDS             complement(64450..65301)
                     /locus_tag="IXT21_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ4867982.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(65314..66099)
                     /locus_tag="IXT21_000550"
     CDS             complement(65314..66099)
                     /locus_tag="IXT21_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4867983.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(66451..67392)
                     /gene="ribF"
                     /locus_tag="IXT21_000551"
     CDS             complement(66451..67392)
                     /gene="ribF"
                     /locus_tag="IXT21_000551"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ4867984.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(67396..68319)
                     /gene="truB"
                     /locus_tag="IXT21_000552"
     CDS             complement(67396..68319)
                     /gene="truB"
                     /locus_tag="IXT21_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ4867985.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(68687..71049)
                     /locus_tag="IXT21_000553"
                     /pseudo
     CDS             complement(68687..71049)
                     /locus_tag="IXT21_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            71497..71670
                     /locus_tag="IXT21_000554"
     CDS             71497..71670
                     /locus_tag="IXT21_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4867986.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
BASE COUNT        19971 a        15434 c        12487 g        23905 t
ORIGIN      
        1 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
       61 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
      121 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
      181 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
      241 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
      301 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
      361 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
      421 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
      481 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
      541 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
      601 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
      661 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
      721 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
      781 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
      841 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
      901 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
      961 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
     1021 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
     1081 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
     1141 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
     1201 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
     1261 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
     1321 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
     1381 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
     1441 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
     1501 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
     1561 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
     1621 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
     1681 cttattggca aatcgccaaa ataatgtaga ctaactaaaa agactaagaa acaataacaa
     1741 aagttaacac ttcttagtct ttctttagca cgtctattta ttaaagattt tagggccttt
     1801 tctcatgttt ttacgatcat tcttaggtgt ttccatttgt ttggatcggc ctccaccttg
     1861 tttctttttg atcaattctt tcattttttc tttttggtcc atttttattc tcctttaacg
     1921 aatgaagcga ctaattcgat atgatgggtt tgcgggaaaa gatctacagg ctgaactttc
     1981 ttcacggcat aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt tgccggatta
     2041 catgagatat agacgattcg ttctggtgac atttcgattg ctgattcaat aaaatgatga
     2101 tctaatcctt ttcgtggagg gtcaactacg atcacagatg gtttgatccc tttttcttgc
     2161 cattttttca taactttttc tgcttttccg acttcataat gaacattttc gattttatta
     2221 tttaaagcat tgaactgtgc atcttcaatt gcttctggaa taacttccat cccatacacc
     2281 ttatccactc tgtctgctaa agacaagcca atcgtaccta ttcctgaata agcgtcgatc
     2341 acaatgtctt ttttatctaa ctcggcaaac tcgatggctg tttggtacag atactctgct
     2401 tgttgagtat ttacttgata aaatgacttt gctgagattc gataggtttt tcctaatagc
     2461 tgatcttcga tatagtctct gccatataaa actttttctt tgtctcctaa aatcacattt
     2521 gttttttctt cattgatatt ttggatgacc gaaaccactt ctggcaattc ttcatgaata
     2581 gcttcagcaa tttccttgga tttaaagaat ttttctttac gagtaactaa aacgaccatc
     2641 atttcatggg aataatgacc acgacggata atgatgtgac gaagctgtcc ttcatttttt
     2701 tcttcattat atcctcgtac ttggaaccgt tgcaagatat ctctgactac tttgattgct
     2761 tgatcgatga caggatcttg gatataaaaa tcttcgatag ggactagatc atggctattc
     2821 tttttgtaaa aaccggtggc taactgtcca tcgatacggc gaacagggat ctgtgcttta
     2881 ttacggtagc cgaatggttc tgccattccg accgttggaa gtacaggaac ttctggcatc
     2941 ttagcaatct tcgccattac atgctctact tgtttttgtt tgaagacaag ctgttgttca
     3001 tacttcaaat gggctaaagg tgcgatacct atgcgaagta atgcattgtt ttctacaggc
     3061 tgacgatcag gtgattgtgt aataaatgtt tcgacttttc catatccaaa cttattgcct
     3121 gcttttacta ctagtatttc tacttcttct ccagggagtg cattttcgat aaataatgga
     3181 taaccatcga tttttgctac ccccattcct tcataactca agtcgacgat ttcgactgta
     3241 tatcgttcgt ttttctttac tatttgctgt tctgtcatat tttgctcctt taagcctatt
     3301 tttcaatcct actgaactct actataaatc agctaattac acaactaaaa ccggcactga
     3361 ccttactacg ttcagcaccg gcttttgatt aattcttttg attaggtgtt tcgtctgcta
     3421 tttttccgtc accgtcgata tcttcatccg ttaaacgttc gacttctttt acgaattctt
     3481 tgctgacaac ctctaattct tcttcatctg tacctgtgat tgcttcatca ggaatctgat
     3541 cggtattggc aaagaactca atgtgttgat gaagattttg gaaatgcatc ggtgcttccc
     3601 cgccgtattc accatctaag ttgatcatta ctggtcgatt gatatcatct tctgtttcaa
     3661 cgaacaaact gcttgttttg gtatagatta ttcttggatc atcgacatgt cgaccgccat
     3721 tcagcatgag tgctgcaata tgcaagatct caaagatatt tgctgtcttc acaatgatta
     3781 aagaaaactt cccatcatcc aatttggcat ctggcgcgat ctgttcgaat cctccaacag
     3841 agttcgtcag ccctaggaaa aacatcgaag catttccgat atattcccct tcatcatact
     3901 tcaagcgcat tttgattggc tttactcgag ggagcatctc tgctcctttc gcaagataag
     3961 caagataacc aaaaatactc tttagttctg atggaacttc ataagtcaat tctgtcaaat
     4021 agccacctgc agcgatattg atgaagtaac tgttatcaga aaggccgatg tccattttta
     4081 cggtttggtg tttttttatc acttctgctg ctgctttaat attatctcta ggaattttta
     4141 atgctcgagc atagtcgttc gttgtaccag ctgggatgat ggccatttta ggacggtgtt
     4201 caagcggagc gattccatta accacttcgt tgattgtgcc atcgccacca gctgccacta
     4261 ctagatcaaa acctagttct gcgactctac atgcttcatt cttggctgaa tctggttctg
     4321 gtgtagttgc ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt aggatatccg
     4381 ccaaattttt tttcaataat tcttttcccg aggtggggtt ataaattacg cgtgcttttt
     4441 tcattttcag cctctatcgt ttcgctaatt catctttaag tagcttattc acgacaccag
     4501 gatttgcttg tcctttagtt gctttcatga tttgaccgac taggaagcca accgcacgat
     4561 cttttccatt tttgaaatca tcgacagatt gttggttgtt atccaaaatt tcattaatga
     4621 ttggcagtag tttgttcgga tcagataatt gcacccaacc atttttttca actacttctt
     4681 gggcttctcc gccatttgtg atcaattcac ggaagacttt tttggcgatt tttgagctga
     4741 tcgttccatc ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc aattttgtct
     4801 cagctaattc cagtttctca ctgttcaagt aagcagaaac ttcacccatc aaccagttag
     4861 atgcttgctt ggcatctgca ccattttcca aaacagcttc aaaaaaatca gacatttctt
     4921 ttgtaagtgt caataccatt gcgtcatact ctggaagtcc aaactcacgg atatagcgtt
     4981 cgcgacgaga agctggcatc tcaggcaaac tagcttttac tttttgaatc cattcgtcat
     5041 cgatcacgaa tttcgggata tcaggctctg ggaagtaacg ataatcacta gacccttctt
     5101 tgacacgcat caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt tcttgttgga
     5161 tttctcctcc tgaaagaaga acttttgctt gacgtttttc ttcaaaagct aaaccttttt
     5221 tcacgaaaga taaagagttc aagtttttca attctgcttt cgtaccgaac tcttcttgtc
     5281 cataagggcg aagagaaata ttggcatcac agcgcataga gccttcttcc attttcacat
     5341 cgctgacttc tgtaaattgg ataattgatc gcaatgcttc tagataagcg tatgcttctt
     5401 ctggtgaacg catatcagct tcagaaacga tttcgatcaa aggcgtgcct tgtcggttca
     5461 aatcaacgta agaataaccg ccaataccat gcatgttttt tccagcatct tcttctaaat
     5521 ggacacgttc gatccggatt tttttctttt taccttctac ttcgatttcg atccatccgt
     5581 catgaccgat tggttgatca aattgggaaa tttgatatgc ttttggatta tctgggtaaa
     5641 agtaattttt ccggtcaaaa tgagtatctt tagagatttc acagtttaat gccaaagctg
     5701 ctttcatccc gaattccagc gcagctttat tcattactgg cagcacgcct ggataacccc
     5761 agtcgatcac gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc gctggagaga
     5821 aaatttttga gttggttttt aactcgacgt ggacttctag tccaatgact gtttcaaaat
     5881 tcattcgtct ttccctccta aaatagctgg ttgttgttta tggaattctg ttgcttgttc
     5941 aaacgcatat gccgcttgat acatcgtttt ttcatcaaaa taattaccga tgatctgcaa
     6001 tccaactggt aatccttctg ataaacctgc cggtacagac atacctggaa gtccagccaa
     6061 gttgacaggg atcgttaaga tgtcactcat gtacattgtg attggatcgt taatgttctc
     6121 accgattccg aatgcaactg ttggtgaaga tggcccgatg atcaagtcat aatctgcaaa
     6181 gactttttca aaatctcgtt tgatcaatgt acggacttgt cctgcttttt tgaagtaagc
     6241 gtcataataa cccgcgctta aggagaatgt acctaacatg atacggcgtt ttacttcttc
     6301 accgaatcct tctgaacgtg agttgacata tacatcatct aatgatttca cattttcaga
     6361 gcgataaccg tagcggatac catcgaatcg ttgcaaattg gaacttgctt ctgatgaggc
     6421 aatgatataa taaacagcca caccgtattt tgaatgaggc aagctgactt cttccacaac
     6481 agcacctaat gaacggaatg tttctgctgc tttcaataca gcttctttta cgccaggggc
     6541 cacaccttcg cctaagtatt ctttaggtaa agcaatcttc attcctttga tgtcgcctgt
     6601 cagtccctct gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt tttcgtcata
     6661 accgctgata gctgtcaatg ctaatgcatt atctttcacc gtacgggtca ttggtccgat
     6721 ttggtcaaga gatgaagcaa aggcaatcaa accaaaacgt gagacacggc cataagtagg
     6781 tttcatccct acgatgccgt taaaagctgc tggttggcgg atacttcccc cagtatcact
     6841 acctaaagaa actggaattt gtccagctgc aactgctgct gcagaaccac cagaagagcc
     6901 accaggtact tttgttgggt cccaagcatt ttttgttttc ttgaaataag aagtttctgt
     6961 acttccaccc atagcaaatt catccatgtt tagcttacca acagggatca tatctgcttg
     7021 atagatttta tccatgaccg ttgcatcata aattggttca aaattgtaaa ggatcttaga
     7081 tgcagcggtt gtcaagatat cttttgttac gatattatcc ttgataccaa ttggaatacc
     7141 agcaaggacg ttgctttcat caatcccttt ttcatctaaa gcttttgcct gttccaatgc
     7201 tttttcttcg ttcaatgtaa tgaatgcctc gacctcaggc tctgtttctt tgattcgttc
     7261 aaatgttgtt tttgtcaaat cttgtacagt gatttctttc gatacaagca tatgatgcag
     7321 ttcttctaga gaacgattgt gtaattctgt cattatgcgc cagcctcccc attatcgatg
     7381 attgcgggta ctttgataaa gccgtcttct gtttccggca catttttcaa taattcatcg
     7441 cggttccatc cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc aaccacattc
     7501 gacgtaaatg gaacaccttc cgtatctact tcttccagca tttcaaccat atcgatgatt
     7561 ttacctaatt ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag ttttgctaat
     7621 ttggcaacgt gtttcacttg ttcttcacta atcgccatac taccccttct ttctaaacga
     7681 gttaaaaact ttgctctcta ttttaacaca agagtgccgt gtctgaaatt gtttattgaa
     7741 atttgtctga aaaaactcat ttttcattct tctactgagc gacttttagc caatgcatct
     7801 gccatttgat cttctgtcca aacttcgatt cctaattctt gtgctttggt cagcttgctg
     7861 ccagcatctt ctcctgctac cacgatatcc gtttttttcg atacgctacc agtcactttc
     7921 ccgccaagat tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt tagtttacca
     7981 gtaaggacta ctgttttttc tttaaaagga gactcgactt cttgcaattg ggcatttcga
     8041 agccctttgt attcgaaatt gacgcctgct tgttttaatt cattcatcaa ttcatggact
     8101 tcttcgttac tgaaataagt gactacactg tcggcaatga tatcaccgat cgtatcaagc
     8161 gcaataatcg actcatagtc acttttactc aatgtttcta gatcaccgaa atgttctgct
     8221 aaaatttttg ctgctttcgc tccgacatgt cggattccta acccgaaaat caaccgctcg
     8281 accgaattat ctttgctgtt gtcgattgct gtcaaaatat tatttgctga tttttctttt
     8341 attttgtcca acgtcaataa ctcttcttct gtcaatttgt aaagatcagc aacatctgct
     8401 actagttttt tatcatacat ctgttctagt acgcgcggcc ccaacccatc gatattcatc
     8461 gcatttcttg aaacaaagtg gttcaaacct tctttcattt gagcagggca tttaggattg
     8521 atgcaccgta aggctacttc ttcatccaaa tggacaagtt cacttccaca aactggacaa
     8581 tgtgtaggca attggtattc ttcgctgtct ttaggtcgtt tatctaacac gacttgcgaa
     8641 acttctggga tgatgtctcc cgctttataa atcagaactg tatctttcaa gcggatatcc
     8701 ttcaacttaa tataatcacc gttatgcagg ctggcacggc ttactgtcgt tcctgctact
     8761 cggactggtg tcatgatagc tgttggcgtt actacccctg ttcgaccgac tgtccattcg
     8821 atgttttcga ttagcgtttc aacttcttct ggcggaaact tgtaagctgc tgcccatctc
     8881 ggtgctttga tagtaaaacc aagttgatct tgtagcgaga attcattcac tttgatgacg
     8941 attccatcga tttcataggg caaatccaca cgtttgtcat gatattcttc aatgtatgac
     9001 cacacttcat caatcgaatg acatagacgt ttttcccgat tcgtgtggaa tccaattttt
     9061 tctagctctt ctagcgcatc atactgggtt tttgcttgca taggaccgaa atcagctact
     9121 gtgtataaaa aagtgtccag atttcttttc gctgtgattt tagaatctaa ctgacgcaaa
     9181 gaaccagctg cggcgttccg aggattcgca aagatatctt ttccctctgc ttctctgtct
     9241 tggttcaatt ggacaaatga ccgttttggc atgaaacatt cgcctcttac ttcgatgttc
     9301 attggttctt ttaatttgat tgggaccgac cggacagttt ttaggttttc agtgatgttt
     9361 tctcctacag ttccatcccc gcgagtcgca cctctcacga aatttccatc ttcataacgc
     9421 aaagaaacgg aaagtccatc tatttttagt tcacagcaat agtcaactac acgtcccact
     9481 gctttttgta cacgttgatc aaaagctatc agctcttctt tgctgaaaac gtcgttcaaa
     9541 ctgtaaagcg gaatatcatg agtgactttt tcaaaacctt ctaatacttt tccgcctact
     9601 cgttgagtgg gagaatcaga cgtgatcaaa tcaggatatt gttcctcgat tgctactaat
     9661 tctgcgtatt ctttatcata aacatagtct tccactgttg gtttatcttc tacgtagtat
     9721 tcgcgagacc attggttcag tcttgtacga agttcttctg ctcgtttagc agcttcgtca
     9781 atcgtcattt catgttccat catttaccct ccactactaa gctttttcaa ttggcgcaaa
     9841 ggcagccaaa agacgtttta ctccttgact tgggaaagcc acatctagtt ctagatcctt
     9901 cgacgtcccg cttactcgga cgactgttcc tagaccccat tttttgtgtt tgactttatc
     9961 tccaggtttc cagctgttat tttcccctcc agtagcactt ttagagggaa caaccgctgt
    10021 ctctggctgt ttatagctat atgctgtttt tttagcgaat acaggcgttt ttttaggcgt
    10081 aggacgcatg ccttcgattt ccagtaactc ctgatcgatt tcttctacaa aacgagaagg
    10141 tcggttatat tgcgtccgtc catataatgt tctggagaaa gcatttgtta aatacaacgc
    10201 ttcttctgct cgcgtgatcc ccacatatgc gagacggcgt tcttcctcca gttcgctttc
    10261 ttccatcaat gctcgagata aaggaaaaac cccctcttct agaccaatca ggaatacaac
    10321 tggaaattct aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg aagcatcttc
    10381 ttctaaattg tcaatgtcgg aaacaagtgc cagatcattt aagaagaccg ttagtttttc
    10441 ttctggtgca tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga attcttgtgt
    10501 gaccgttaaa aattcctcca agttttcgat gcgtgcttgt gcttctaatg tattttggat
    10561 ttttaagtct tctagatatc cgctacgatc caaaacctct ttcgtcaatt ctgtcactgt
    10621 caagtatggg atcatctgag tgacttcttg gatcatctct ccaaatgcac ctaattgctg
    10681 tcctgctttt ccaccgatat ttgccagatc cacattttgc gctgcttcaa gcagtggcca
    10741 ttcatgaaga gaagcaaaac tgcgtagttt ttcgatagat cctggtccga tgcctcgttt
    10801 gggcgaatta acgatacgct caaaactgat tgaatcttga ggatttacca gcacattcaa
    10861 atacgctaaa atatctttga tttctttacg atcgtagaac ttgtgccctc caaccatttt
    10921 ataaggaata tttgctttca acagagtttc ttccattaca cgagattgtg cgttcgtccg
    10981 gtacaagatg gcaaagtcgc cgtaattccg atgattgctt cgaatctctt cttgcatccg
    11041 gtctacgata aaacgggttt cgtctcgttc attatctgca cggtagtaag tgatcttgtt
    11101 tccttctttg ttttctgtcc acaagttttt tggttttcga ttgctattgt tttcaatgac
    11161 ttggtttgct gcgctaagga tgtttttcgt ggagcgatag ttttgttcta gcaaaattac
    11221 tgcagcatct ggataatctt tttcaaaatc caagatgttc tgcatatctg caccgcgcca
    11281 gccgtaaata ctttgatctg catctcccac gacgcacagg ttcctaaatc tacctgccaa
    11341 taaattgact agcgtatact gtgcgtgatt tgtatcttgg tactcatcta catgaatata
    11401 atggaatttg ttttgataat acgtgagtga atcaggatgt tcttcaaata atcgaatcgt
    11461 attcatgatc aggtcgtcaa agtccataca ttgattatat tgaagctctt tttggtaagc
    11521 agcataacat tttgccgcaa tctcttcgta ataactgcct tgcatttttg caaaatcctg
    11581 aggggtctgc aggctgtttt ttgcttgaga gattgttcct aatatacttc gaggatcata
    11641 ttttttcgga tcaatgttca gttcttttag tatccgtttc atcaaggtca gctgttcaga
    11701 agaatcgatg attgtaaagt tccggttata cccgataaaa tcaacatctc ttcttaaaat
    11761 acgcacacac atcgagtgaa aagtcgatac ccagacatct tcgcctccag atgcgagaat
    11821 cgcattgaca cgttctttca tttcttttgc agccttattg gtaaaagtaa tcgctaggat
    11881 gttccaagga ttgacttctt tttcctcaat caaataggca atgcggtgtg tcagcactct
    11941 cgtctttcca cttcctgcac ctgccattag cagtaatggt ccgtcagtgt gaagaacagc
    12001 ttctttctga cgggggttca tcccgtttaa taattcagct ttggatgaca ttaacaacat
    12061 cctttctttt atcaatcttg ttaattatag cattttcttg gctttcatta aactagctag
    12121 ttcaaaatca tttgtttctt tttatggatt tttccttgtc aattcgttga tttcgcgcta
    12181 aattaaaaga aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga aattagataa
    12241 gactcagata tattttattc taaaaggaat atttgttgga ctgctgacag gaatcgtcgt
    12301 tagcctgttt cgattatcta tcgaaaagct aagcgattta gttcgaagta tctatgaaat
    12361 gagtcgggaa cagccgattt acttaattgg cctagctatt tgctgcatcc ttgcagcatt
    12421 tttcgtgggt tatctggtca aaaatgaacc ggatatcaag ggaagcggga ttccacaagt
    12481 agagggacag cttcgcgggg aattatcaat gaactggttc tctgttttat ggaaaaagtt
    12541 cattggcggt gtactttcag ttggtgcggg cctttttcta ggtagagaag gaccttctat
    12601 acagcttggc gcatccattg gacaaggagc tggacaattg tttcggagtc catcttcaga
    12661 agaaaagatt ttaatttcta gcggtgcaag tagcggactt gcagcagcat tcaatgctcc
    12721 tatcgcagga ttactctttg ttttagaaga aatccatcac tccttttctc cacttgtttg
    12781 gctgacatca tttgcttcag caatcactgc taacttcgtc tctctttact ttttcggctt
    12841 gagacctgtt ttatatctag gtgaccttcc ttctttgccg ttgaaatatt atggttcctt
    12901 agtgatctta ggagtacttc taggcatctt aggttttatt tatcagaatg tcttgctggc
    12961 tttgccaaaa ttttacaacc gattgccttt accagcttat tttcacgggt tcgttccttt
    13021 cttgctgatt ttgccaattg gttatttatg gcctagtact ctaggcggag gcaatcaaat
    13081 cgttttagct tttggacatg cttccttgcc aatcattatg attatcggtt tgtttgttct
    13141 tcgattcgtt ttttccatga tttcttacgg tgggggtctt ccagggggga ttttccttcc
    13201 gatattgaca ttgggggctt taatcggtag cttttatgga aacattatgg ttgatttcgg
    13261 gatggatcca atctacatca aggattttgt cgttattgcc atggctggtt atttcacagc
    13321 gattggaaaa gcaccgctga cagcaattat tttagtgaca gaaatggttg gctctcttaa
    13381 tcatctgatg cctttaggat tggctgctct tgttgcttat atcgtcaatg accttctcgg
    13441 cggtaatcca atttacgaat cattattgga acgtctactt ggaaatactc agaccgcctc
    13501 actaaaaggt gcaaggacaa cttttgattt tccagtaacg gcagagagtt ctttagacgg
    13561 attaatggta cgagacttca attggcctaa aaatatgctg ctaatctcta tacgcagagg
    13621 aaatcaggaa atcttgactc acggtgatac gatcatgaat gtaggagata ttttggttat
    13681 cttgactgat gaagcaaact tgccaagaat caagcaggaa atcgaggcta aatcttctat
    13741 cacaaatttg aagcaatcat tttaacgaac atggaaaaac tattttacaa caactattca
    13801 tctacactac ttgattctat atgattctat aaaaaaagaa gaggctggga aaacccagcc
    13861 tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata aaccataaat
    13921 cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg ggtgactcag
    13981 taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga aagctccgac
    14041 taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa tcgggtcagc
    14101 tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga tgttcgtcaa
    14161 accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag caacgaaaat
    14221 tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac ttccgccagt
    14281 tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt tgattggacc
    14341 gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag cattcgaacc
    14401 actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag ggacattgac
    14461 cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga ggatcgtttt
    14521 aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata cgatgacata
    14581 accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg tattagctaa
    14641 tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg aagcaataaa
    14701 tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt tgaaccagct
    14761 agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc caataaattg
    14821 gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag gtaacatgtg
    14881 tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat ccgcatttcc
    14941 ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac cactaagggc
    15001 tagattgatc aactcttctg gtttacggat accatcactg acaggacgat tgatcagttc
    15061 ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta caccttctgc
    15121 acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt tcgtttcgac
    15181 tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg ccatgtaagt
    15241 gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt ctggatgagc
    15301 agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt tttgctcttc
    15361 agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta attttcctac
    15421 gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat gtgtgtcgtt
    15481 tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt ctaaggcttc
    15541 ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa ctgcactgtt
    15601 tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg cttcgcgggc
    15661 tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa acattttttg
    15721 gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga tcatcgcttc
    15781 tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc aatttttgtg
    15841 atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc atcagaaaaa
    15901 gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc tttcgtacgg
    15961 ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga atttttcaat
    16021 gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa agcaccgtct
    16081 ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt cccatacgga
    16141 atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc gtgattgttt
    16201 ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc tgttgtatca
    16261 atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata aaatcctttt
    16321 cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc tactgttcct
    16381 aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa gccattgatc
    16441 tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt tacggttgtg
    16501 aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc agtgaaccct
    16561 cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg ggaaacattg
    16621 atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc gcctagtttt
    16681 aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt gtaaatcatt
    16741 gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct aatggacaac
    16801 gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac aactggttga
    16861 atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct aaacgtttaa
    16921 cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca ccgttcattc
    16981 ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta gatcctaaaa
    17041 cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc tccaaatcaa
    17101 ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt ccttgcagaa
    17161 aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct tcgtcattaa
    17221 tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg agtgattttt
    17281 cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca tgaactcttt
    17341 ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat tcagaagcat
    17401 tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc aagtctagga
    17461 ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa ataatagcat
    17521 attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt ataaatcagt
    17581 caaaaaaaaa ataaaaaacc agtcagaaag actggttttt tatttttttg tctatcatca
    17641 agccgtttta tcgtttttta ttttcgcttc ttgttttttt catataaaca gaagcaggtt
    17701 ttctttcctt atgaagaaga taataataaa gtcccatccc gccaaacatg accaaaaaac
    17761 cgatgactga tcgaatagga aaattaggaa caaaaaagag acagacggat aataatgatg
    17821 ccggaaccat tattcgataa tagagttgtt tatccatttt cttcacctca tattttttgt
    17881 aaagtaatca attgttctaa ttctcggtaa tctaaaaatt gcacacgcag atgctgctca
    17941 cctttcttga catcgaattg gatacttcgc cgctttttct ttattcgttt gaaggtaaca
    18001 tcagagagtc tatgtttgat ttcctttaac ggtaattctg acagggattc tgaaggaaca
    18061 tagattctta aggataaaag atactcttga tatttttcat ctgttacctc tacttctaga
    18121 tattccctaa tataataaca ataagcttcg attccattat ccaaagcaac agtcggatcg
    18181 ataactttat agcacatcat gcgtgatcga ttctcgtcaa cccaaggaaa atacgcctga
    18241 gcatttagtg ttccgcgaac acgcctttct ttataatctc cttgttggat atcataactt
    18301 tttgttacaa aaaatgaagc attcttttta ggaacaggtt gcatttgtgc attctgatac
    18361 tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa cgaatggcgg tttcaataaa
    18421 ccgacaccat tgtcccaagc aagatgatca aacccaagct ggtccctaaa ataggattga
    18481 aacatttgat tgtttggctt ttgccagtta aaggggtctt gataaagctg gatacatatg
    18541 acttcgtttt ctaattcctt cactttttct acttgttgga tgtcgagaaa aatcggttta
    18601 gggatgattt gatgataata gcgactataa tacatttccc aacacgaagt gaaacataaa
    18661 ccttggtaaa aaagatcgta tcctggaaat tggttgcaat cgactactac ttccccgttt
    18721 tgttgtttca ttttaggtaa atgttcgatt tcatcctttt tctcaaacaa tagccgttga
    18781 gagatctgtt ttgtattgtg atagataaac tcgctataag ctcgcatata ggcgaacacc
    18841 ccatgagcaa tcattttttt ctgaatgaag tctcgaatca aaaattgctt ttctttgaaa
    18901 acagagatag acaatacatt ttttatcaat aattgattat cttccaagcg aaaactacat
    18961 tcgttctcgt catttcctac aactaaccaa ctttcgtctc ttaccgatgc tagcattatt
    19021 ttttcttgaa gctttcgaag atcatacgga tccgcttgtt gtgtgaaaga gcggataaat
    19081 gttggctgga tcaacagacg tgtcaaaata gcccaatcgc ttatttcctg ttttttttct
    19141 tcttcattta agtctgtgag ctggatagtc atcaataatt tatcttcgcc aatcatttcc
    19201 tcacctcttt caaatctttt tataatttaa ttataaccaa tgataaagaa aaactaaaac
    19261 tttatgtctt agaaaccaat ttggacgatt acatgataga ctaagtaaaa agaaacaaag
    19321 aggtgtttac aatggggttg aaatttgaac gtacagataa tttagataaa cttttcgaat
    19381 catttgctgt cgatccggaa aaaacagttg caccaaaaga agagatcgat cgatttctaa
    19441 aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca aaagtaagtt aagctcaaaa
    19501 aaagacttac cagccaaact tttatgaaag agtttgactg gtaagtcttt tttattcttt
    19561 ttatgattca attgcttgtt cgattgctgc ccacgcttct tcttttccct gttttgtaac
    19621 ggaagaaaag agaataaacg tgtcattgcg atcaaaatcc aacgcttttt tgatttcgct
    19681 ctcatgcttg ttccatttac cacgagggat cttatctgcc ttagtggcaa caacaattac
    19741 agggatatca taatacttca aaaattgata catctggata tcttcttgag aaggcttatg
    19801 acgcaaatct actaaagaaa cgaccgcacg tagctggtct ctagtcgtta aatatgtctc
    19861 gatcatcttt ccccattttg ctcgctcagt tttggatact tttgcatacc catatcccgg
    19921 aacatcgaca aaatgtaatg cgttctcgat caaataaaaa ttcaatgtct gggtctttcc
    19981 tggcttaccg gaagtacggg ctaaattctt tcgatcaatc aatgtgttga taaaagaaga
    20041 tttccctaca tttgatcgtc ctgccagagc tatttctggc aattgtgtat ctggatattg
    20101 ttttggtgcc actgcactta tgataatttc agcttgatgg actttcatgg tctctctcct
    20161 taacaaggtg atgaataaag caagcggctg tcgcaaaagc caaaatgact tttacggcag
    20221 cctcttttca tcaacccgct tttttctctg aatctttata gattactgtt ggctcaccag
    20281 acaattcagc tgcttctttt gtgataatca ctttttcgat gttttcattt gaagggatat
    20341 caaacatcac atccatcatg atttcttcaa tgatggaacg aagtcctcga gcaccggtat
    20401 tgcgttcgat tgcttttttc gcaatggctc tcaacgcttc aggttcgaat tcaagttcgg
    20461 tattatctag agaaagaagt ttttgatatt gtttgactag cgcatttttc ggctcagtca
    20521 agatccgtac cagatcatct gttgtcaatt tctctaaagc tgccataaca ggtaatcgtc
    20581 cgataaactc agggatcaat ccgaatttta gtaaatcttc aggaatgata tgctgcatga
    20641 cactttcgtc ttcagaaagt ttttggtttt gtgtaccaaa tccaatcgtc ttttcgccca
    20701 tacgattctt cacaatcgtt tcgatgccat caaatgcacc acctacgatg aacaatatgt
    20761 tcgtcgtatc aatctgaata aattcttgat gtggatgttt acgtccgccc tgaggtggca
    20821 cacttgcaac agttccttct agaattttca gcaaggcttg ttggacacct tcgccggaaa
    20881 cgtcacgagt aatagataca ttttcgcttt tacgcgcaat tttatcgatc tcatcaatat
    20941 agatgatccc tttttctgca agttccacat tgtaatcagc agattgaagc aatttaagca
    21001 aaatgttttc tacatcttcc cccacataac cagcttctgt caaacttgtc gcatctgcga
    21061 ttgcaaaagg tacattcaat gtttttgcta atgtttgtgc taaaaatgtt ttaccagacc
    21121 ctgtaggtcc gatcaagcag atattgcttt tttgcagttc aacatcatct gtgttttctt
    21181 gtgcttgctg attgacacgt ttatagtgat tgtatacggc aacagctaac gagcgttttg
    21241 cgcgatcttg tccaatcacg tagttattca atacttcaag aatttctagt ggtttaggga
    21301 cttcagtgaa ttcgcgtaca gcttcttcat aaaattcttc atcaatgatt tctttacata
    21361 ggtcgataca ttcattacag atgtagaccc ctggaccagc tacgatcttt ttcacttctt
    21421 cttgggtttt cccgcaaaaa gaacagcgta cggtctcatt gctacttgga ttgtcataca
    21481 tggtcttcac cccttaactt gcaaaaagta tgcaagaaac tatcaaagac tttacagtct
    21541 tttactatca taacatagtt tagtaaaaag aaaaagtgtt taacaaagga tcaactgacc
    21601 tatacctttg cattttttac tcttaacaaa aaataaggaa ctgtgacaaa atcgtgtcac
    21661 agtcccttat ttcgaataaa caattttcta gaagtagctt cttcagaaat gatttattac
    21721 ttgatttcta ttctttcgct tctgtcgctg tacctgtgat caattcgatt gcttttttca
    21781 ttgtcacatc atgttccagc atatcttctg tcaacacgcg tttgatttgt tcgattggca
    21841 tgttgtatgt ttctgataat tcttggattt ctgcattgat ttcttcttct gttgcttgaa
    21901 gattttcagc agctgcaatt gcttcgatca caagatttgt tcttgtgcgc atttctgctt
    21961 caccttcgaa ttgtttatgc aagtcttctt ctgtagaacc agttaattga tagtacattt
    22021 caggagagat accttgacgt tgcatgttgt tcaagaattc gtccattgaa cgatgtactt
    22081 catcatgaac catcacgtgt ggcaattcaa cgatttctgc gttttcaaca gccatacgga
    22141 tcgctgcttc atcttttgca tcgtccgctg ctttttcttt tgtttctact aattctttgc
    22201 gatatttttc tttcaattcg tctaatgttg atacttcgtc atcaacatct tttgcgaatt
    22261 cgtcatctaa ttcaggcact tcttttgctt ttacttcatg gattgtcact ttgaagactg
    22321 cttcttttcc agcaagatct tctgcttgat aatcttctgg gaaagttaca tttacatcta
    22381 gactttcacc tgatttatga ccaactaatt gatcttcaaa acctggaatg aatgagcctg
    22441 aacctaattc aagtgagtaa ttttcgcctt ttccgccatc aaacgcttcg ccatctacaa
    22501 agccttcgaa gtcgattact actgtatcgc cattttcagc tgcagcatct tctttgataa
    22561 ctaattcagc ttgtgcttct tgttcacgtt tgatacgtgc atccacgtca gcatctgtta
    22621 cttcacggtc ttgtttttca acaactagat ctttatattg tcctaatttt acttctggtt
    22681 tcactgttac ttcagcagtg attacccagt cttgaccttt ttccatgctt tcaacatcga
    22741 tttttggttg agaaacagga tcgatgccag cttctttgac agcagcttca taagcttctg
    22801 gtaatacagc attcaatgca tcttcatata aagcttcttc accgtacata cggttgaata
    22861 cttgacgaga aactttccct ttacggaatc caggtacgtt caagcttttt ttcactttat
    22921 taaacgcagt tgttaatcct ttttggattt cgtcttgcgc aatcgtaaat tttaaaacac
    22981 catcattcgt gccttttttt tcaaatgttg cagtcattta attccctccg agtttagaaa
    23041 ttcattttat acatcaaaaa cgtttcatgc atacctttaa atagtacact aaccctttca
    23101 aattgtaaac atattgaatg gatagaatcc ttgtttttca taacattttt atctttttca
    23161 ctgataaaat ctaatggaag aagatcattt gctgcaaaat tttttctctt ttttttaaaa
    23221 aatcttgttt ttcatcttct gtaccttcat acatcgtttt tcctgtacac gcctctacag
    23281 tcagttgaaa ccagcgcttc gggtcttcga tgtatgtatc atagacagga taaacaattg
    23341 cagattgcaa cgtgaattct tcttttaaca cggaactaag cagcgcatct tcattcccgt
    23401 aacgatctga aaatacacat aattgctggt aacttgcctg ctgctgcggc tttggcagat
    23461 cttttggtat gactttgact aacttttctt caatcgtcaa atagaaaaca ggatggcatt
    23521 cgttcaattg cctaagtgtc tccagtaatt ttgcccttgc taaaatatgg acctctggcg
    23581 atacaataag tttactcgct gtttggataa actcatgata aggaagctgt tcgatctcat
    23641 caatagacgc aagctgacgt gcaggttcca acgaaggaag ttcctccgat ttattgtgga
    23701 gtgccttgat cttctgcctc tccatttgtc cggataattc ttctgcaaat tggatttttt
    23761 caatgacccg ctgctcgaaa gcttcataaa aatctgtact tgctatcaat tttctagcat
    23821 ataaaaaatc ttgtgtactg attaacaagt caaaataaaa ttcagcaaac tcctcatttt
    23881 gaagatactg tttttcatgc aagagtgctt gcttcaatgc ttcttttttc tcgtccaact
    23941 cgtaaagaca aaaaacaagc aggctattag cctcaaagct ttctttcaac tcgtaagctc
    24001 gttgaaataa ttcttttgcc tgctgatatt gctggtttgc tagtgcttgt tgtgccactc
    24061 gcattaaatt ctcatatttt tctggaaaat caatcggctt cacgcagtgt cctcctcttg
    24121 ctcatattca gctcatgttc aatagtagca aaacaaaaaa agcatgacaa ttaaaaataa
    24181 ctgaagcacc ctctaaaagc tagattttag atctgacttt tagaaggtgc tttcaactgt
    24241 tactgctttt tattgctcat cagctgttga cttcctggtt gaacgctttg atcagattga
    24301 tcgtaacaac acttgtatta gtgaaaaaat cgcccagatg ttgctttttc tgattaaaga
    24361 ctgctggaag ataccctaac atcagcaaag gaaattttag aatgaaccgg caagcacctt
    24421 ctctaactaa aacggttgac cagctaagtt cttcttcatc aaagctgatg actcgaatac
    24481 caaagatcat tttcccaatc gtttggccat gattgaattt cgtcaataaa ataaaatacg
    24541 ctagataaat aagtaaggct gtgaatccgt agagactcag ataagaatct gaaaaattaa
    24601 gccaacctag atttttggca ataccaagtg ttaaacgcgt aatagctccg atacacaaaa
    24661 gatcgaccaa gaaggcaaac attcggataa aaaaacctgc atagaaatac ttaggaaaat
    24721 catttgcttt tggcggttca tttcttgtgt ccgtatactt tttccattta cgttgtttct
    24781 ttttgatttc ttcgggcgtc aaaggctctc tttcaaaaga gctcagtacc ttttgggaaa
    24841 agttggagtc aggtcctaca gcaggctgct gttgcttatc tttttcttgt tccttatttt
    24901 catttgaaag ctctgacatg cttactcacc tccgtaataa tacattgctt ttggtgattc
    24961 cgctgtccct agattctcaa gaacggataa gatctgacta gtctcagaag gcttcaatcc
    25021 ctggaattct gctagtttac ttcctaacca tgtactagca aacccagatg gcgtggaaga
    25081 atattctact agttccgcat cttcaagctg ttgttccttt cgtatagctt ttaaagcatc
    25141 ttcaggaaat cccaattcat cgactaaacc tacttctttt gcttggacac catcataaat
    25201 ccttccatca gcaatttttt tgacttcttc ttcagattta ttccgtccct cgctgacgat
    25261 tgctacaaaa cgactatatg cgctgtcgat atatgcttga agcaccgctt cttcttcttt
    25321 tgtctctggt cgcgttcctg aacccatatc tttcaacgct ccgcttttaa ctgttgtatc
    25381 ttccacaccc agtttttcta gaagtcctga ataattcaat cctgacatga tcacaccaat
    25441 tgagccggtc actgtttctt ctgttgcaaa gattttatct gcttgagcag aaatatagta
    25501 gccaccgcta gctgccatgt ttttcatgct gacatacatt gggatatcat gttctttacg
    25561 aatcgtatcc aatttcttag cgatttccgc actttcgtag acaccgccac caggagaatt
    25621 tacttctaaa aagatacctt tgactgttgg atcttcttca atcgccttca gttgttccat
    25681 aaaattttta tgattgtaac cttcagaagc aaataaacct gaagaccctc ccgcagcaat
    25741 cgttccatct actgataatt ttacgatttt ttgcgtactg tctccctctt ccaacacatt
    25801 tggagctagt tcttcatccc catataacca actgttcaat ccctcaattt gatcgttttc
    25861 ctgaggctta gtcaaactag ctgaaactaa cgaaaccaca aataatcctg cagcaatcag
    25921 aactgctgcc caacgtcttt tattcattct ttctcctttc taatacaagt tttttatgac
    25981 gatcacagtt tctgactgct cagaagtgtc gaaccgctct gctttttctt cccgcgcata
    26041 cccggaaaac gactcttttg ttaattcatc ttctaccgta aactcacgag cttgagcaac
    26101 ttccaaggca tcaggtgcga tttcttggat gctatcccag ccagtatcta aaaatatttt
    26161 cgtatcatct gatacaccag ctgatgcttc gaatttggac aattgttttt ttaacattcc
    26221 cagtgtcatg atctcaattt ttgaatccat ttttactcct cctctatccg attactttct
    26281 attgtaccga actcctgaaa aagatgatag ataaaaaccc attatttttt gacataaaaa
    26341 ggacatttga caaagaattt gatatatacc aatttatttt ttattctttt tttgttgaca
    26401 agaacattcg caaaaggtta tgattttaaa tgtttccaga taaaaaacaa aaaggaggat
    26461 agttatacct tttaggatag cgccaggtct gtgtaaacag atgacgagga gagggtttat
    26521 cgaaagattc ggcggatggc cctagggact gcactctaca gaaaatccac aaaaaatgtc
    26581 tgcaaagata aaacaaaagg attttcctgc agctggaagc attcatacga attcattata
    26641 gaaagtagga tgttttttat gtgcggaatt gttggaatga ttggattaaa aaatgtaaca
    26701 ccaggattga ttgacggtct agaaaaatta gaataccgtg gttatgattc agctggtatc
    26761 tttgtctcag atggcacaac tgattatctg gtcaaagcac aaggcagaat tcaaaatttg
    26821 aaaaacaaaa taaatattga tactacagga aatataggta ttggtcatac acgatgggca
    26881 actcacgggc aaccttctga agaaaacgcc catccacata cttcccaaag tggacgcttc
    26941 gtgctcgttc acaacggcgt aatcgaaaac tttgaagaac ttaaaattgc ttatttagca
    27001 aatgaccact ttattggtga gaccgatact gagattatcg ctcatctaat tgaaactttc
    27061 gcgaaggata ctacaacgca agaggcgttt ttaaaagctt tgcgggtcat caaaggttct
    27121 tacgctttcg ctttgattga tcggacagca cctgatgtta tttacgtcgc taaaaataaa
    27181 agtccactat taatcggact cggcgatgga ttcaatgtga ttgccagcga tgcaatggct
    27241 atgcttgctc atacgaagga attcgttgag attgaagatg aagagatggt taccgtcaca
    27301 tcagaaaaag ttattattca aaattttgca ggtgacatcg tagaacgttc ttcattcgaa
    27361 gcacaagtag atgcttcaga tatcgagaaa ggtacctatc ctttttatat gctgaaagaa
    27421 atcgacgaac agcctattat catgcgtcgt atcgctcaaa aatacgtaac agaagacaat
    27481 cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg atagcgatcg aatctatatc
    27541 gttgcttgcg gaactagtta ccatgcgggg ttagcaggta aacagacttt ggaaaaattg
    27601 acacagattc ctgttgaggt tcatttagcc agtgaatttg gctataacac acctcttctt
    27661 tcaaaaaaac cattctttat tttcttgagt caaagtggcg aaacagcaga cagccgccaa
    27721 gtgctggtaa aaatcaaccg tctcggctac ccttcactta cgattacaaa tgttgctggt
    27781 tctactcttt cgcgggaagc atcctttaca ctgctattgc atgctggacc cgaaatcgca
    27841 gtagcatcaa cgaaagctta tactgcacaa atagctgtac taactttatt agccaaagct
    27901 atcggtgata aaaaagaatt tgaaacttct cttgtattcg atatcactca tgaattgagt
    27961 ttagttgcta atgcgatgga atcggtcatc gcccaaaaag attatttaga agagttggca
    28021 gaagtatatc tcagcgatac aagaaatgcc ttttacattg gacgtggtgc tgactacaac
    28081 gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt atatccaggc agaaggtttt
    28141 gccgctggcg aattgaaaca tggaacgatt gctttgatcg aagaaggaac accagtgatc
    28201 ggaatcatca gtgaagaagt gactggtgcg catactcgag gaaacctgaa agaagtcgaa
    28261 agccgtggcg ctaaaacact agttattgtc tcagagggat tagaaaagga aagtgaccag
    28321 ctggtgctac caactgtcca cccttatcta actactctaa ccacagtcgt accgactcaa
    28381 ttacttgcat attacgcaac gctattacgg ggattcgacg ttgacaaacc acgtaattta
    28441 gcgaaatcag tgacagtaga ataataaaag aacaaggacc agtgtcagac atctgacact
    28501 ggtccttgtt cttttatttt ttatgaaaat atctcttaat ccagtccgat ttcttctttg
    28561 actactgccg cgattttatc gacatagtaa ttaactttct catcagtagg cgcttctgcc
    28621 atcactcgaa gtaatggttc tgtaccggaa ggacgaacta ggattcttcc ttctgatccc
    28681 atttcagctt ctgcctcttc aatcactttt ttgattgccg gaacatccat cgcaccgttt
    28741 ttatcactta cgcggatatt cactaatttt tgcggataga ttgttacctc tgctgcaagc
    28801 tctgataatt tcttacctgt ctgtttcatg atattcagta actgaattcc tgaaagcatc
    28861 ccatctccag ttgtgttaaa atctaagaaa atcatgtgcc cagactgctc gccgccaaag
    28921 ttataatcgt ttttgcgcat ttcttctaca acgtaacgat caccaacttg tgtaacgaca
    28981 tctttcaaac cgattccttc caccgctttg tggaatccta agttactcat gacagtcgta
    29041 acgatcgtat cttttttcaa tcgatttttt tctgccaagt atttagcaca gataaacatg
    29101 attttatcac cgtcgacaat gtttccaagt tcatccaccg caataatacg atcgccatcg
    29161 ccatcgaaag ctagtccagc atctgctcct ttttcaacga ccatctcagc taaacgttct
    29221 ggatgggtag agcctacgcc gtcattgata tttaacccgt ctggatttgt gcccattgta
    29281 taaaaatctg tttcaagatc ggcaaacaaa cgattaacac ttgttgaagt tgctccgttt
    29341 gctgcatcga cacaaatggt caaaccggaa aggtcaccgt tgatcgtttg tacaaggaat
    29401 tgagaatatt tcaataagcc ttctggaaat tcttctacag ttcccaatcc ttcagctgat
    29461 ggacgaggca attcatccac ttcagcatct aataatgctt caatttctag ctcttggtca
    29521 tctactagtt taaaaccatc tgcaccgaaa aatttgattc cattatcttg tgctggatta
    29581 tgagaagcag aaatcatgac ccctgcgctt gctttttgca aacgtgtcag ataagcaaca
    29641 cctggtgtag agatcacacc tagctggaac acttcgatac ccaccgataa aagtcctgaa
    29701 atcagcgctt cttctaacat ttgacctgag attcttgtat cccgccctac taacacgcgt
    29761 gggcgttttc cttcttcttc atgctggctt aggacataac ctccgcaccg tcccagctta
    29821 aatgcaagtt ctggcgtaag ttccttgttg gcttcaccgc gtacgccgtc tgttccaaaa
    29881 tatttaccca tagttgtaaa attactcctt taacttaatt ttctgaggaa tttgttctgc
    29941 tcatttgtgt tgattcgctt gtttctgatg tttcttctgt acttgtggtc gagctgcttg
    30001 tcccagattc cgaactggaa gaaatggttg ttgtcgatgg ttccgttcct gaatcagcag
    30061 atgaaatact tctactcgta cttgtatttt cttgtgtccg actgctcgtc gaagcagagc
    30121 tactgcttga cgtttgcaac acgggtgtaa tctccacact aactgattcg ggcttgatac
    30181 ttaacccctc ttctactggt atttcaattt tacgagttgt tcgatgattg atacctgaga
    30241 cattgatcgg tacagcaata ctgtcaccga tcgatgcaag caaatcgcta gagccgctta
    30301 cttctgcttc cagttttgaa aaactgaaat catagctttc taccccggca gctggtgttc
    30361 cttgctgtat cccataaagt cgtaccgact tgcttggctt gatcgaatca gctctcactg
    30421 ttacttgcac tggatcagag acgatactta atacttcccc tgctgcattc agcgcctgta
    30481 ttgggacctc actattaatc ccatcatctg tgaccatcga agcgtccaca tcagctacta
    30541 ctcgtttgat ttctgccaat gttttgtcac ctgtagtgat agagactttt ttaggactaa
    30601 cggaagtatc atcaagttca tatccagatg gtgtgaacgt tgaaggaatc aacgtttcca
    30661 catcgaaatc ttttattacc tttttctcaa ccgttactgt aatagtcgat ggttcgatct
    30721 ttgctgtgac agcactgctc aaattttgaa tttttagcgg aacttcatgt gttccttcgc
    30781 ccagcttagt gatatctgct gtgacgcgga acatccgtgt atcttcgttt gcctctgcat
    30841 tcaactgaac tcggtttgca ctggaaagtt tgacagtcac tgcattttca tatccatgga
    30901 tatagtagcg ctttgaatca taagaaatct tgatcggcac ccccgtaact gtttgattat
    30961 acgcttcatt tccagaaagc gtatttggaa cggactggcc atttgcattg aagtacagcg
    31021 caatactgaa cagcaatgca ataaaactgt aaagaatgct tgtccgtttc tcttttgaga
    31081 tcatttgcga tcccctccct tagtcatacc gtctaggaaa gattgaatca cgtttttctt
    31141 gttttttgtt ttctcttcag caattagttc tctacgcaag attgccaaat attcttgctg
    31201 ggataagcca gccatcagtt cattatctaa cgtgatgctg acgtccccgg tttcttcaga
    31261 gacaacgatg gtaatcgcgt cactgacttc gcttataccg acagctgccc ggtgtcgagt
    31321 cccgaattct ttagggatca acatactttc tgatagagga agataggcgc ttgctactgc
    31381 gatctttcct tcttttacaa taactgcacc gtcatgcagc ggtgtattag gaataaagat
    31441 attgatcagc agctccccag tgatatctgc atctaaagca atccctgtct caatatactc
    31501 atctaatcca gtatgacgtt cgatagtgat caacgcaccg atttttcgct tggacatata
    31561 ttggatggct ttgtcaaaag acaagatcat cttctcatct tcctgctgtt cgctttttga
    31621 ttgtttgaaa agcgaactgc gtccaagatg ttccagtcct ctccggattt ccggctgaaa
    31681 aatcacgata gcagctatca ccccgtacgt aatgacttgg ttcatcagcc aagacaaggt
    31741 gtgcagacca atgatttccg ctacgattcg gataacaata aaaatagcta cacctttcag
    31801 aagctgaatc gcttttgtgc ctcgcagcag ttggatcagt ttgtatacaa gataccagac
    31861 aaccagaata tcaagtatgt tcaccaaaaa attcagagag aacaagtcac cggaaaacat
    31921 ttgctgccaa tagtccaaat taaaaagttc cccgatttga aatgacataa aaagcctctc
    31981 ttctcttttg agatttcctc tctagtatat catatgtttt aattttagag gctgatatgc
    32041 tcgtattttt cttgagaaaa acgcataatt ttcaagaaaa ttgcaagtat gcgttttatt
    32101 tttctctgca ataaatattt ttaaacttca gagaattatt cattatattt tttctgttat
    32161 ttttcctttt tttcttttgt agcttgttcc atttttttac gtcgttctgc tctggcagct
    32221 cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc tagtcttaac gggtacatcg
    32281 atagattcct ctggttcaag tcgtttcttg attttttcta attctcccgt cagtgcagca
    32341 tagttgtaaa aacgtgtttt agcgtcttgg atagtctttt caaataaagc ttttgcaaga
    32401 tttggatttg cgttctctag cgaggaaaag cgaacttgtt tcctcatgaa atcaggcata
    32461 gcagagaaat caggtttttt gtagtccaaa gtcattgggt tcttgttttt ttcgaccatt
    32521 gcagggttat atcgatataa tgaccaatag ccagaagaaa cagcttcttt tgcttcttta
    32581 agcgattctt tcattccacc aagcaaacca tgtgtgatac aaggcgtata ggcaatgatt
    32641 aaagaaggtc caggatactt ttctgcttct tcaaaagcct ttatcgtctg catttgattg
    32701 gcacctgaag caatttgcgc cacataaaca tttccatatg tcatcgccat cattcctaga
    32761 tctttctttg aagcatactt tccactagcc gaaaatttag cgattgccga agctggtgtt
    32821 gcttttgagg tttgaccgcc agtattggaa tagacttcgt tatccaaaac aagaatattc
    32881 acgtctgctc cgctggcaag tacatgatcg atcccgccaa aaccaatgtc gtaagcccat
    32941 ccgtctccac caatcatcca ttggctgttt tttacgaata gatcctttct cttgtatata
    33001 tcctccagtt cagggacaat tgatcgttcg ctttcgatcg cagcttgcag ttttgctgct
    33061 ctttgctgcg ttccttcgct ctcaaacatg tgtaccaacc agtcttccat caattctcgt
    33121 agttcttctg atacttttgg caatacttct ttgattgaac ttgccaaagc ctcgcgacga
    33181 gtctgtgccg ctagaagcat accataacca aattcagcat tatcttccaa taaagaattt
    33241 gaccatgcag gcccttgacc acattcattt gtcgtataag gagaagcagg agaagcaccg
    33301 ccccaaatag aagaacagcc tgtcgcattc gcgatcatca ttcgatcacc gaacatttgt
    33361 gtcaataatt ttacataagg tgtttcgcca cagcctgagc aagcgcccga gaattcaaga
    33421 agcggttgat tgaattgtgt agacaagaca gtattctttc ctttgactgg atttgctttt
    33481 tgtcttaatg tcattgcaaa agcccaattc acagcttgtt ctttctgttc ttcataaggc
    33541 ttcatcacta aagctttttc ttttgcggga caagcctcta cacacagacc acaacctgta
    33601 cagtcttcca ctgaaacttg gatacggtat tttacgccgt ctgcgcctcg catttctctc
    33661 acgataaatc cttctggcgc ttcttccatt tcctcttcat ctgctaaaaa tggacggatg
    33721 gcagcatgag gacaaacaaa ggcacattca ttacacatcg tacagcgatc actaatccac
    33781 tcaggtactt ctaaagcgac gccccgcttt tccatagaag ccgttcctaa aggcatttcg
    33841 ccagttgtca ttccatttgc tacaagtgta ccaacagaga gttgatttcc ttcctgtgca
    33901 ttcactggtt caagtatttc ttgtacataa ccagtgttcc ttttattgat tttttcaggc
    33961 atctccaacg ttctccaatg gtctgggatc gcgacttgat gaagcagttc ggtcgtccgg
    34021 tcgattgctt gtatgttttt ttctacgata gccattgatt tatgtccata gcttttcagg
    34081 gcttcttctt ttagcagcgg aatcacttca tcaaaaggaa tgatgtcagc taatttaaaa
    34141 aatgccgttt ccattgccgt attgatccgg cgtcccaaac cgacttcttg ggccaatctc
    34201 atggcattga tcgtatagaa tctaatgtta ttttcagcca agtaacgttt taattttaag
    34261 ggcagatgcg tttctaattg ttcgtcgctc caaagagtat tcaataaaaa aatgccacct
    34321 ggcttcaatc ctttgaccaa gttatagcta tgcaggtaag ctggtgcgtg acaggcaaca
    34381 aggtccgcat gctcgacaag atacgcagaa cgtatcggcg tatctccaaa acgaagatga
    34441 gagacagtca gaccaccaga ttttttcgaa tcataataaa aatagccttg cgcatattta
    34501 tcggtgtgat ctccaataat cttgatagct gatttatttg ctccgactgt tccgtcagat
    34561 ccaaaccccc aaaacttggc ctggaatgtt tgcggttctg tcaaatccag actttccttt
    34621 ggttccaaag attgataagt cacatcatca acgataccta tggtgaaacg ttttttgcgt
    34681 atgcttgggt ctttttgcaa ttcatcaaaa actgctgaga tttggtcagg agtcacatct
    34741 ttagaaccca atccgtagcg accaccaata acagccgggc gaagttcaga atcatataac
    34801 gcactttgaa cgtctaataa gagaggttct ccacctgccc ctggttcttt acttcgatca
    34861 agaacagcaa ttgtttttac tgtatgaggc aacttttgca cgaatgtttc cactggaaaa
    34921 ggacgataaa gatggatatt caaaaaacct gttttccgtc cgtttgcatt caagtaatca
    34981 atcgtttgtt caatcgtctg tgcaactgat cccatagaaa caatgacttc ttccgcatcg
    35041 tctgctccgt aataagtcac caagtcataa gaagttcctc gtaatcggtt gatctctttc
    35101 atatattttt gaacgatttc aggtaattta tcataatagg ggttgatcgt ttcccgttgt
    35161 tgaaaatgga tgtctggatt ctggttcgta ccactaacag tcggatgatt gggattcatc
    35221 cctcgtttac gaaatgaagc taatttctcc tgattgatca gaggagctaa ttcttcatat
    35281 tccaaaactt cgattttttg aatctcgtga ctcgtacgga atccatcaaa gaaattcaaa
    35341 aaaggtactt ctccttcaat agaagcaaga tgggcaacag gtgctaaatc catcacttct
    35401 tgtacactgc tttcagctaa catagcaaat cctgtttgtc ttgcagccat cacgtctccg
    35461 tgatctccga aaatattcaa cgcatttgtc gtcactgccc gactagcaac atggaaaaca
    35521 cttggcagta gttccccagc aatcttgtac atattcggta tcatcaaaag cagcccttga
    35581 gaagcggtat atgtagttgt cagtgcacca gttttcaatg atccatggac cacaccagct
    35641 gctcctgctt cggattgcat ttctactact tttactggtt gaccgaatac attttctttt
    35701 cctgctgctg accattgatc tactaattca gccatggtcg aactgggtgt tattggatat
    35761 attgcagcaa tctcggtaaa tgcataagaa atatatgcgg ctgccgtatt gccatccatt
    35821 gttttcattt ttcgcatgat tcatcatcct tgttcatatt ctctcttatt atctgttcca
    35881 ttataacgta aaacattcca ttttttcttt tatttataaa aatatttcac atatgtctat
    35941 ttgaattggt catctaattt ttagacaaac agtctaaatc gttgcctttt tttgtcagac
    36001 atgtataaac tataagcgaa agaggtgttt taacatggat atatcagtga ttgatgcaac
    36061 gaaaacaaat acacaaaaag gaatccttta tggttcatcg aatgctccta aaaaaatggt
    36121 cgaattcatc aacctatctt gtccgtattg ccgacaatgg tttgaagaat cttatgagct
    36181 attagaagaa gcagtccaaa gcgggcaatt gcagcgagtg atcaaattgt tcgataaaga
    36241 aaaagaaagc ttgcagcgag gaaacgtgat gcaccggtat ctcacaatca gcgatggtca
    36301 aaaagcgatc aaagagatca aacaaatatt cgatactcaa gacgagtgga aacatctttc
    36361 tctccaagaa gtagcggatt ttgcagtaga taaactaaag ctgacagaac aaaaagacga
    36421 acagctgagc caagctgtca tcaacgaagc tgagcaagca catattcgat ttgttccgac
    36481 tgtcatcttg ggtaaagaaa tctttgatga atctatttca atagaggaat taaaagaatt
    36541 gatccaaaca aaataaaaaa ataaggagcg taggacaaaa gtaaagaaca cttttgtctc
    36601 acgctctaaa cacctataaa cggcggaagc agaagcaact ccttcttatt tcttggagtt
    36661 aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc ttgcatctgc
    36721 cagattatct catttcttat gacaagctga gcaaattatc tccaccaaat aatttattca
    36781 tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt tctcaaattc
    36841 ccttaacgag taatatttat gtacggtgat tcacactagt atcccaatca ctttttaagt
    36901 tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt tagttgatta
    36961 gatggtcgtt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt agcttatacc
    37021 tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg tatttgccac
    37081 tttgattcgt ataaagctaa caactcctaa ctcctctcat ttaatagttg ctctcttttt
    37141 tattcagata catttttgct gatcacagcc tagtatttca atggttttga acactaaacc
    37201 aaattgttcc attacaacat tcactggctg atgaaatgga ttaatagatt tcgattattc
    37261 aaagcgattt tctattggtg gattgtgttc ccaactaaca agacggattc caaagtcatt
    37321 atataactct tcaagagtta accctttcct gttcccaata gatgtatctc tcccacgctc
    37381 gatataaaat atggagtttc gaatgccaag acctggcaaa tctttccttg ttacagttac
    37441 actgctacta acttccccat ataaaatcca gctcctccaa aatgataata tatttttcga
    37501 ccatcatcta actcatgtaa caataattgt tgtgccctct tgtcactcgc caaatgcaaa
    37561 cgccacgtgc gataccatgc catgctacct gcatctgcag aaaatccatc aaataatgca
    37621 acaattgtat cttctatcac aatcctttcc ccatctactt catattctaa tctaaaatca
    37681 aactcccctc gggtaatcgc aggtcttggt ggcttaggac ccaatattat tctacttaaa
    37741 accgcattcg ttcccagatg aaacacagct ataacaacaa taatgattaa tattttgttt
    37801 ctatttgata aatttctcat tattcgcaaa tcacctctac attctcacat actagttcgt
    37861 aatactcctt taatatcaaa atatctactg atcgtaataa tattcgctat gaattcctac
    37921 tactcgtata agaaaggcca aatcatcagc ttttacttgc tgatgatttg gcttgtttct
    37981 atttatcgta attgttttct aatcaccaaa attgttaaca atcggttcag atatttccca
    38041 attcactagc cttatattat attgatttaa caattcttct tcatttattg tattttgatc
    38101 aagcggacca aattgttcta ataattcttt gtcatgtaaa atatcccttc tagtaatgcc
    38161 tctttccctt tcaatgaaag aagcactagg aaaagtatga ttataaccat tcaacctatc
    38221 tcccatgtaa taattagcag gtcctggaaa ataaaatatt tgcttattat cactaacttc
    38281 taacagcaac actaaatctt ctccgctggc taaacgactt gtccatctac gatatctccc
    38341 tatgccttcg ttcattccaa atccatcaaa ttcaactatt aacgtgtctt ctatcacatg
    38401 taattcttca ttcaattcat actcaagtct aaaaggaaat tctccatatg taatctctgg
    38461 caatggtggt gctggagttc tccaaataaa tacaataact gtgaatagaa aaaatccaac
    38521 gatcgatata aatgttatga gacctgctcg aagtaaaacc cgatataata aaaatgctgt
    38581 atttcgcttt gctatatcta gaaaaataaa aagtactgca atcataatga tagtaaaagt
    38641 tgtaaaaaaa cgcatttttg ccctcccttt ataaacatcg attctcaaat tctaaaccaa
    38701 atcttggtag ttcaagttca ggtataacgt attccaacca tacatctcaa ttactattta
    38761 tgaacttatg attatcactg aataaatcac tccatcccct tgttaaaact tctacattaa
    38821 ttacacctgg ttgttttcga ctagctaatc caatgaactt atcaaccatc cttatccacc
    38881 aaccacgaga atcttgacca gaagtaagaa gagcagttct caaggatgat cgagcagttc
    38941 cttccagttt tttaattaat caggccagtt atagataaat acttgatctt atctatatca
    39001 ggacttctat attaagcaaa aatttatgta caaaactaaa atcttcattc agttgtctcc
    39061 ccgcttttta tacgattgta ttaaaaacaa tagaagcctt tccaaattat gttactccag
    39121 taaagatgga attatagttt gacggaagaa aagcaagtat aacttttaaa aaaaactgat
    39181 acattcgcat ctcctgcttc atgtatcagt tttttctttg gattaaactg cttcttcacg
    39241 cagtttttcc gcaaattcat ttattttgcg aattcggtga ttaattcccg attttgatat
    39301 cgcacctgag ggaatcattt cccctaattc tttcaagctg acctctggat gttgaagccg
    39361 aagttctgcg atttcctgaa gtttttcagg aagggcatgt agaccgacct ttgcttcgat
    39421 caattcgatg ttttcgattt gtttagaagc cgcatcaatc gtcttgttca gatttgctgt
    39481 ctcacaattg actagacgat tgaccgaatt tctcatatcc cgtacgattc gaacatcttc
    39541 aaatcggagc attgagttgg ttgcaccaat caatgttaag aaatcagcaa ttttttctgc
    39601 tccttttaaa taagaaatat agccattgcg ccgttcaagc gttcgagcat gtagaccgta
    39661 ataatttaac atatcgcaaa tgtcttggtt atgctgttca taaatagaaa atatttccaa
    39721 atgataacgg ctcgtttccg gattattgac tgaacctgag gccataaaag caccacgtaa
    39781 gtaagaacgc attttttgcg aatttcccat aatctcgtcc gaaacgtgtc cattgaacat
    39841 cataccatcc atgatatcaa gatccatcaa cacatctttt gtgccttgct tcaaccggac
    39901 gatatagaca ttgttctttt ttaatttcat ttttttacga acgagtagct cacttcgtac
    39961 ctgataatga tctttcaaaa gagaatagat acgtctggca attgcagcat tttctgtttg
    40021 gacgttcaaa ataaactgct ggttcacaat acctaaagag ccgttcattc gcagcaaagc
    40081 agctaattcc gctttcgcat gttctcgatg aacttccaaa ccagttagtt ctttcttcac
    40141 atctgcagca aatgacatcc tattctcctc ctttctccaa caataaattg cttaatattt
    40201 tgaaccgaaa actaaacgga acaattcttc tactactttg tctccatcgt ggaagacacc
    40261 accatctttc aattccaaaa agtcggtgga aatcactcga catccttcat ttctcaatcc
    40321 gttgaagtcg tgtgcgactt gtactagata ctcatcatag atctctgggt ccatataatc
    40381 ttcaggcact ttttccgtat tgaccaatac tgtatcgata aaaggctgat tcaaatgctt
    40441 gtgtaaaaca cgtatatgat cggcatctgt gaaatgttcc gtttcccctt tttgggtcat
    40501 gatattacag atatagacgg tttcagcagt tgtcttgagc attgcttcgc cgatttctgg
    40561 tatcactaaa tttggaagga tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc
    40621 ggcgtccata attgctgaaa cgacttttcg agccgctttg gcttcacttt tatcatttgt
    40681 gttgcagaca tacacacggt caatggtttt acggtcgatc gcgattttag attctcctac
    40741 tgcagtcgtt ccgtctttaa agacagcatg caaggtcaaa ggagtttctg aagaaggata
    40801 aacatgtcca tctacatgca tcatttttgc taataattga atcgcctcat aagtgcttcc
    40861 ccgcatctcg gaaactgcgg caatgatcag attaccgatc gtatgattgg ctaaaaactg
    40921 atcttccttt ttgaaacggt attggaaaat atcttcatag aattgtggca tatccgataa
    40981 cgccactaag acatttctca agtcaccagg aggagccata gcgatcgatt ctctgattgc
    41041 accgctgctt cccccatcat ctgcaacagt cactactgct gtgatatctg ctccttgatt
    41101 tcgcaaactt ttcaggataa ctggcagacc agtgccaccg ccgaccacta cgatctttgg
    41161 ttttcttatc cggtaggttt tcattttcac gaccggttca ccgtctcttt ccttttatcc
    41221 ttgtcccgat gtgtcacatt gactttatag tttttcccta aagcatttgc caaacgttca
    41281 gtcaatgcca cagaacggtg ttgaccgcct gtacagccga ttgcaactgt caagctgctt
    41341 tttccttctt taatgtatcc aggcaataca gtttccagaa gatccaaaaa tttatggtag
    41401 aatgattgcg tttcatcaaa ggacatcaca taatcataga ctggctgatc ttttccggtc
    41461 aatggtctta gttccgggat ataatgaggg ttgggcaaaa aacgcacatc catcacaata
    41521 tccgaatcga tcggcaaccc gtatttaaaa ccaaatgata caaattcgat ccgaaatcct
    41581 tggtctccct ttgatttgaa tgactcattg attttttcac gtaattgtcg tggtgtcaat
    41641 gtcgtcgtat caatgatcac ggaagcttgg gctttcaagt cgtctagtat cgcccgttct
    41701 ttacgtatcc cttcagtgat caaaccgtcc atcgccattg gatgtgctcg tcttgtttct
    41761 ttgtagcgag ccactagttc ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg
    41821 aagttcgtat tttcgatttc gatcagcatg ttttggattt cttcaaagaa ggaacgagaa
    41881 cgtaaatcca ctcctaaagc catttttgtg actttgcctg attctttgat taattcccag
    41941 aattttggaa tcaatcgagg cggcatatta tcgatacaaa aatagcccat atcttcaaaa
    42001 ctttggatag ctactgtttt ccctgcaccg ctcatacctg ttatgatgac taattgcaaa
    42061 ttatctgtca taattacgaa ctcctctcac attgtgcata gtaaaattat atcattccgg
    42121 gcgtatcatt accagttttc tttttctttt acaaaaagat ttggaccaaa tatcttttag
    42181 gatatttgcc ccaaatccaa tttttttact tttccgatgc tgtatccaat gccttgcttc
    42241 tttcagctag cgctcgttca atcgtccgat ctgctaaaat agattgggca tcgatcactg
    42301 gataatggtt atcttcagca aattcatgca tcaatgacaa ttctgtgcaa cctaagataa
    42361 ctttttcaca gtttaggcgc tctaccgctt cttctaagat ttcgtaatat agctcttgat
    42421 tcaaataatc cgattccttg atctcatgat agatcagata attgattttt tcttgaagaa
    42481 cagtatcagg aatcactgtt tcgaatccaa gatttttgac ttcgcgttca tagattccag
    42541 ctttcatact tccttctgtt cctagaatcg ctactctccc tgtcgtatgc tgacggacaa
    42601 gttcatttgc tgcttctctt ggcatatgaa ggataggaat atccgttgcc gcttgtaatt
    42661 cttcaaaaaa gtagtgtgcg gtgttacaag tcaacacaat gaagttcggc tgcaataaat
    42721 tttgtttctc aatatcatcc agcagaaacg gcattggatt ttcttctgat cgatctaaaa
    42781 tataagctgt acgatcagga actgttgcat gattgaataa gacataattc aaatattctt
    42841 gatcttttgt agctttcgtt cggtggttga tcaagcgaac aaaactttct gtcgccatcg
    42901 tgcccattcc gcctaaaata ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa
    42961 aatacttttt gaatcttgga atatagttat ggaacatata tttcatcaac aaccagcgct
    43021 tgattgaccg gtctttttca taaaacacag tagtaccgta acgattttct ttgatcattt
    43081 gctcagctag ttttttgtct tcattttccc gagcatattc taaaaagatt tttttgggca
    43141 cacccatcca taatcttgct ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg
    43201 gtttgttaaa cactcgatct tctgtgacga aacgagccag attcaagccg ttcaatgtca
    43261 caaagaaact gctacgacct tgtctcaaat tgatttcaaa taatttatat tctccatctc
    43321 taggatcata cttcatatcg aaattggcaa agcctgtata ttcgattttt tccaaaaatg
    43381 ccttaatggt ttgatagatt ttttcgttat aatctggcat gatcacaacg taattaccaa
    43441 tcgatgctgg tgtaggatct tctaacagtg gatgaccaag acacatcatc cgaacttgat
    43501 gatcttcatc tacatacgca tttagcacac gcatattact atcatcaccc gggataaaat
    43561 cttgtacaat catttcactt ttataaccgg cttcgtatat ccggcctaaa attaaatcga
    43621 actcttctct agtctctaaa ataaaagctt tttttcttcc ttcaaattgg acagaaagat
    43681 actccacact gtttgcaggt ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca
    43741 aatgtccatt tactaacatt tcttctctga cgatcaatgt tttaggatac ggaagatcat
    43801 attcttcaca aacttcataa aagcttactt tattgatcag tctttcaaat aaagaataat
    43861 caatatatgg acagataaaa tattctgaca gctcttgttt atgttgggag atcaattctg
    43921 catatccatc accacaagca atcaaaagat attttttact cttatcagaa tatctttctt
    43981 ttgccaaacg aagcatcgtc tcgataaaca cagggtcttt atcaaatcct ggaataactt
    44041 ctacattgac aattttactg tagcgggtag gggctaactg atccgaagca tatgcttggc
    44101 agattttgcc gtatgcttca ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc
    44161 ctaataatat agggataaat tcttcatttt caatactgtt catcattatc tctcacttac
    44221 tcataatctc ttgtttttct tttccaacta cacaaggaat atcataccat aaagaaaaaa
    44281 gcaatcaaag gaaactcctc cgattgctca attctttttt gaacggattg aacttatagg
    44341 aagtttttgt ctataccaat cagtgtagaa agaactacca tgtaatgatt ctagaacact
    44401 gatattaata aaacaagcaa aaattcattt ttttatgttt ttttaactat ctcagctatc
    44461 tatcctcttt ataccattat ttttttcaaa tagtgacctg tataactttc ttttacgtta
    44521 atgatttctt ctggtgttcc agtcgcaagg atcgttcctc caccttctcc accttctggt
    44581 cctaaatcaa tgatataatc tgctgttttg attacatcta gattgtgttc aatcactaaa
    44641 actgtgttac cagcatctac taatctttgt aatacatgca acaagcgggc gatgtcatcg
    44701 ctatgaagtc ctgtcgttgg ttcatctagt atatagaaat tctttccatt agagattttg
    44761 tgcaattcac tggcaagttt catccgttgt gcctcaccac cggacaatgt cgttgctggt
    44821 tgccccatcg tcacataacc taagccaaca tcaacaatcg tttgcagttt gcgatgaatc
    44881 tttggaatgt gcttgaagaa ttctacagca tcttctactg tcatttccaa aatatcagca
    44941 atgctttttc ctttgtaatg cacttctaat gtttcagagt tatatcgttt gccatgacat
    45001 acttcacaag gaacatagac atcaggcaaa aagtgcattt cgatcttaat aattccatcc
    45061 ccgcgacaag cttcacaacg accgcctttt acgttgaaac taaaacgccc tttcttataa
    45121 ccccgcattt ttgcctcgtt cgtttgagca aataacccac ggatatcatc aaatacactt
    45181 gtataagtcg ctggattact tctcggcgtc cggccgattg ggctttgatc gatatcgatg
    45241 atcttttcga tactttcgta gccggaaatt gtcttgaatt tacctggctt agcagaattc
    45301 ttatttaatt tttgcgctaa cgatttcttt aagatactat tgaccaatgt actctttcca
    45361 gaaccagaca cacctgtgac tgctacaaat tctcctaatg gaaattcaac actaatattt
    45421 ttcagattgt tttctgaagc accagtgatc ttgattgctt tcccattacc tttacggcgt
    45481 tcttttggta cagggattga ttttttccct gaaagatatt tccctgtcaa agaattttca
    45541 tcattagcta cttcttcagg agtgcctgca gccacgattt gtccgccttg ttctccggcc
    45601 ccaggtccga cgtcaatcag ataatctgct gcccacatcg tatcttcatc atgttcgact
    45661 acaacgaggg tgttgccaag atcacgcatt ttctttaatg agccaatcag tcgatcgttg
    45721 tcgcgttgat gcaatccgat cgatggttca tctaaaatat acaaaactcc agaaagattc
    45781 gatccaattt gtgtagccaa tctgattcgc tgcgcttctc ccccagataa tgttccggaa
    45841 gcgcgactca aagttagata gtctagtccc acattttcca agaatgaaag tctgtcattt
    45901 acttctttta aaatcggttt ggcaattact ttctcttgtt cagataacgt taagtttgag
    45961 aagaaatcta ctgctttttg aatagacaac tcatttgttt gcccgatatt cgttccattg
    46021 atttgaacag atagagcttg gggattcaat cgataacctt tacatgtctg acaagttaat
    46081 tctgtcatat aaagacgcat ttgctcacgc gtgaaatcac tgttcgtatc atgataacga
    46141 cgctcgatat tcttcaatac cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa
    46201 tcattttcat aatggaaatg gaaaagatca tctgtcccat tcaagatgat ctcctgatgc
    46261 tcttccggta attcttcgaa tggtgtatct aaatcgatac cgaaactttc acaagcctgc
    46321 gctaacattt gaggataata ctgagagctg attggattcc atggaacgat agcaccttga
    46381 ctaagtgtct tcgttctatc tggaatcact aaatcttgat cgacttctaa tttgacacct
    46441 aaaccatcac agtctggaca agctccaaaa ggcgcattga atgagaacag tcttggttct
    46501 agttcgccaa cagtaaatcc gcaataagga caggcatagt gttcgctaaa caacatttct
    46561 tcttcaccga tcacgtcaac cagagcatag ccgtctgcta atcgcagggc cgcctctaaa
    46621 gaatcaaata ggcgagaacg aatcccttct ttcacaatga tccggtcgat gacgatcgcg
    46681 atatcatgtt ttttgttttt ctccagctca ggcacttcac ttaaatcata gatctcacca
    46741 tcgactcgaa cgcggacgta tccctctttt tggattcgtt cgaatatttt tttatgttgt
    46801 ccttttttct ttaccacgat cggtgccaac acttggatct ttgtgcgctc aggcaaagcc
    46861 attacttgat ctgccatttg atctactgat tgactggtaa tctcgatatg atcgttggga
    46921 caaatcggat gtcctacacg tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca
    46981 gtacctactg tcgaccgagg atttttactt gttgttttct gatcaataga aatcgccgga
    47041 ctcaatccgt cgatgctatc tacgtccggc ttatccattt gtcctaaaaa ctgacgagca
    47101 taagcagaaa ggctctctac gtatctccgc tgcccttcag cgtacagcgt atcaaatgct
    47161 agggaacttt ttccagaacc ggacaaacct gtaacgacca cgaatttatc tcgtggaatc
    47221 gttacatcta tatttttcaa gttatgggca cgtgcaccat gaatcgtaat tttatcgtta
    47281 gccatgttgt cctcctactt tgaagctttt aattctaaaa tcatatctcg cacattcgcg
    47341 gctgtttcaa aatccaaagc ttttgccgca tctttcattt ctttttctaa tttcatcaat
    47401 aacgtgtctt tttcttcctt cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta
    47461 tcatcttccg attctttcgt aatagaaatc aaatctcgga tttctttaat gatcgttttt
    47521 ggaacgatcc catgttcttc attgtacttc tgctgaatcg tacgacgtct tgacgtttca
    47581 tccatcgcta gacgcataga atctgttact ttgtctgcgt acatgattac tttaccttct
    47641 tcattccgag cagctcgtcc aatcgtttgg actaaggaac gttcactacg taaaaagcct
    47701 tctttatccg catccaagat agcaacaagg gaaacttcag gtacgtctaa tccttcacga
    47761 agcaaattga ttccaactaa cacatcaaat tcacctaatc gaagatctcg aatgatttct
    47821 gttcgttcca gcgttttgat atcactatgc aaatatttga ctttgatccc taattctttg
    47881 aaataatctg tgaggtcttc tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg
    47941 tctttttcga ctcgttcatt tatttcgcct actagatcat cgatttgtcc catgattgga
    48001 cgaacttcga tcactgggtc aagcagccct gtcggacgga tgatttgttg gataacggta
    48061 tctgtttgtt catgttcata tggtcctggc gtcgcagaga cataaatgat ctgattgaca
    48121 tgtttttcaa actcttctaa ccgaagtggc cgattgtcta aagcagatgg caaacggaaa
    48181 ccgtaatcta ctagcatctg ttttctcgct cggtcaccat tatacattcc tctgatttga
    48241 ggcatggtta cgtgcgattc gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat
    48301 agcgtataag gcggttcacc ttctttacgt ccgtccatat gccgagaata attttcaatt
    48361 cccgacgtgt agcccatttc taacatcatt tcaatatcgt aattggttcg ctgttctaaa
    48421 cgttgagctt ccagaagttt gttttcattt cgcaagacag ttaaacgctg ttctaattcc
    48481 gctttgatat tcgctacggc atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc
    48541 gggaagattg ctacgtgttc cgtttctcct aaaacttcac ctgtcagtgc attgacttct
    48601 cggatacgtt cgacctcatc tccaaaaaac tccacacgta aggcacgttc atctcttgaa
    48661 gccggaaaaa tctccacgac atcaccgcgc actcgaaaac gaccgcgctg aaaatctata
    48721 tcatttcttt cgaattggat actgactaag tcacgaatca actgattgcg atctagttct
    48781 gctccctgtc gaatcgaaac tacttgtttt tggtactcaa aaggcgagcc taatccaaaa
    48841 atacatgaaa cggatgcaat gacgatcaca tcatttcttt ctaataatga actagtagcc
    48901 gaatgtcgta atttgtcgat ctcatcattt acacttgaat ctttttcgat atacgtatcg
    48961 cttgaaggca cataagcttc aggctgataa taatcataat aagaaacaaa atattcaaca
    49021 gcattgttag ggaaaaattc cttgaattcc ccgtacagct gaccagccaa tgttttgtta
    49081 tgggcaataa tcagtgttgg tttattcact ttctcaataa gattggaaat cgtatatgtt
    49141 ttccctgttc cagtcgcacc taataagatt tgtgcttttt tcccgccgac tacgccatcc
    49201 actaattggt taatcgcttc aggctgatca ccagccggtt ggtatttcga gacaagctca
    49261 aagtgtcggg aggtatctct ctcaatcatt cttcgtcctc cttttcattc attccttttc
    49321 attttacgac caataaaaag aaaaggaaag caaaggcatt ccttactaaa aaattatacc
    49381 acaccgaaca tactttcgcc acttttttat cttattatac atgttatgtt ttataacata
    49441 aaatgttaga aactcgcatt tttttcattt tttcgagctt acagtcttgt ctgattcagc
    49501 acttttcgcc tataataata aaatattgga aatttaggag gttgattatg aaaagaaagc
    49561 atttatttca ctcatttgtt ttcttaatga caggacttgt gacacttgcg tttggtcatg
    49621 ttgcacaagc tgacaacact acaacggcag agtcaagcac aagcgagaca atgcaaaaaa
    49681 tagagcccaa aaaagacgta tacgtaattg ccagtgactc tgcttttgct ccatttgaat
    49741 ttcaaaacgc aaagggcgag tacgaaggca tcgatgtaaa attgatggaa aacatcgcca
    49801 aactgcaagg atttaacatc cgaatggact accgtggttt ctctgctgct ttgcaagctt
    49861 tggaatcagg acaagcagac ggaatgattg ccggaatgac tgtaacagat gaacgaaaga
    49921 aaagctatga tttttctgat tcttactttg atagcgggat ccaattagct gtcaaaaaag
    49981 gagatacttc gatcaagtct tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg
    50041 gtaccgaaag tgctgaattt ttaagcgacc atgaatctga atatggctat tctatcaaat
    50101 attttgatgc tgctgatcaa ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga
    50161 tggatgatta tccggtaatc ggttatgcga tcaaacaagg acaagaattg gaaacaccga
    50221 tcaaaaaaga atctggcgga aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc
    50281 ttcttaatat gttcaatgaa ggattgaaag aattaaaaag aacaggcgaa tacgataaga
    50341 ttattaaaga atatgtagcg gatggttcag aaacttccag tgagtcttcc actgctgatg
    50401 aatcaacatt tcttggactt atccaaaaca actatcaagc attgttgaaa ggactttgga
    50461 gaacaattct cctaactttg atctcctttg ctttggctat agtaatcggt gtgatttttg
    50521 gattattcag tgtagcacca atacgcgctc ttcgcacaat tgctagtatt tatgtcgata
    50581 tcatccgagg aattccaatg atggttcttg cgttctttat tttctttggg ttgcctggta
    50641 tcgttggatt tacgattcct gacttccttg ctggtgttat caccttgact cttaacgcaa
    50701 gtgcttatat cgcagagatt gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg
    50761 aagcttccag aagtttaggt ctttcttata atcggacaat gcaaaaaatc attttgccgc
    50821 aagcgatacg catcatgatc ccatcattcg tcaatcagtt cgttatctca ttaaaagaca
    50881 caacgatcat ttccgcaatc ggtgtagtcg aattgctgca aactggtaag atcattgttg
    50941 cacgtacaac acaaagtacg tatgtttact tgatcattgc gatcatgtac ttgattttga
    51001 tcacagcatt gacaaaatta gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa
    51061 aaaatcttag ttgagcattt agtcaaaaaa tacggcgata acacagtatt aaacgacatc
    51121 aatgtatcca tcaacgaagg agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa
    51181 agtacattct tgcgttgctt gaatcgttta gaagaacctt ctgacggtga aattattatt
    51241 gacggcgccc acttgatgga taaaagcaca gatatcaact tggtgagaca acatatcgga
    51301 atggtattcc aacactttaa cctattccct catctttcta ttttggaaaa catcatttta
    51361 gcaccgatgg acctgaagaa agaatctaga gaagaagcag agaaaaaagc aatcaaatta
    51421 ctgcagacag ttggtctaga agataaaaaa gatatgtatc ctgaaatgct ttctggtgga
    51481 caaaaacagc gtgtggctat tgctcgtgct ctagcgatga accctgacat catgcttttt
    51541 gatgagccta cttctgcact cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa
    51601 aagttagcag aacaaggtat gacgatggtt atcgttacgc acgaaatggg gttcgctaaa
    51661 gaagtggcca atcgcgtcat gttcattgac gacggaaact ttttagaaga tggaacgcct
    51721 caacaaatct ttgaaaatcc gcaaaatgaa cggacaaaag atttcttgaa taaagtatta
    51781 aatatttaat aaaaaccaaa aatgcacggt cgccatgaag gtaccgtgta tttttatctt
    51841 cttattttct cacctaatcg tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat
    51901 cgctctacct gttccatcgc ataagaaagt accatcggtt cattgatgat atcaagtaca
    51961 tagtcgatac caccctcact taattgagct tgatttttct tctccatatt atgtagttgt
    52021 gggacaactt tctgacaatc agcaattgtg tcaagaacgg atcaatcctt tattggagtt
    52081 gctcgaaagg gtcttctctt attacggaca ggctttgctc actgtacata gacaacaaat
    52141 cattatccta gtcaatcgga tttccagagc ctcattgcta tcattgcttg ataaaatcca
    52201 gacaaagttt aataaaatgt atcagttaca gctaaacttt ggaattgggt cgctttgtta
    52261 tacagagcaa gagacaccgc aatctttcct tcatgccaaa caagtttgcg aatggatagc
    52321 tttccatcaa tctgtgaacg agatccgctt ttttgaagat ttagacctcg ggatcgtttt
    52381 accagcaatc ccgtcagatc aacgaacatt atatgtaaaa cgtattttga aaagcctgac
    52441 agaagaagaa gtacatttgt tcaagaagac tttggcatgc ttcagtaaaa ataacggaag
    52501 catcaaaaat tgctcagaag aattatttat tcataaaaac accttgcaat atcgattgaa
    52561 caagttccat tctctcactg gttatacccc tagaaactat gacgattatc atattctaaa
    52621 gctggctttt ttgttggtac aaacatgaca actacaaaaa aaccaggcga acatatgatt
    52681 gttcgtctgg tttttaatta ttaatgactt agcgttttta ctagaaaaac tacttccgct
    52741 ttttcccgcc aaaagcatct ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa
    52801 ctttgatgcc gccagcttca gcgaatgcac gtaatgcttc tctttgttgt tcatttagat
    52861 ttttcggtgt gatcagtttt actttgacat gttggtcgcc tgttgcacca ccacgtaact
    52921 tcggagcgcc ttttccacgt aaacggaagt tcgtacctgt ctgtgttcca gcagggatct
    52981 tcaatttcac atctccatgg acagttggta cttggacttc atcccccaaa gctgcttgta
    53041 caaaacttaa aggcaattca tagtaaattt cagaaccttc acgatcaaag atatcacttt
    53101 cttctacacg gaagaccaca tacaaatcac caaatggtcc gccgttttct ccagcttctc
    53161 cttgaccagc taaacgcatt tgttgtcctt cttctacacc agctggtaca ttcactttaa
    53221 ctgaatgtgc tttcttctca tgtcctgagc catgacaggt tggacaaggt tctttgattt
    53281 ctttccctgt cccatgacaa acatcacaag tttgacggct catcactcgt ccaagtggtg
    53341 tttggcgttc tacattaatc gtacctgcac catgacattt atgacaagta gttggttgtg
    53401 tgcctggttt tgcaccgttc ccaccacaag tatgacagat ttcttcccga ttatacttga
    53461 tttctttttc tacaccaaaa atcgcttctt caaaagacaa gtcgaccgta tattgcaaat
    53521 cagcaccttg acgaggcgca tttggatcga ctgaccgacc gcctcctcca aagaaagagt
    53581 cgaagatatc ttcaaaacca ccgaagccgc caccagagaa gccgccgaat ccaccaaatc
    53641 cgccaccagc tccaccagca ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat
    53701 atgcagcgcg tttttgcggg tcacttaaaa tctcatatgc ttctgatact tctttgaatt
    53761 tttcttccgc atcaggttct ttattgatgt caggatggta ttttttcgaa agcttacgat
    53821 aagctttctt tatttcatct tctgatgctc cttttgagag ccctaagact tcataataat
    53881 cacgttttgt tgccataggc tttcctccta ttggttctca tcattttcac gtcgcttgtt
    53941 atcatatcat attttgtttg aaacaaaaac cgatacttca aactattttt caaaagaaaa
    54001 agccaaagta gcaactttgg ccttctctcg aaattcagtg aactgattat ttatcgtcgc
    54061 catcgacttc ttcaaaatca gcatcaacga catcatctgc tccgccttga gctgcttctg
    54121 ggttttcttg agcttgttgt tgtgccgctt gttcgtatag tttaacagtc aagttttgaa
    54181 cgatttcgtt caatgcatcg cgtttagttt tcatttcttc gatgttgttt gcttcaacag
    54241 cggctttcaa ttcatcgcga gctgtttctg cttttttcac ttcttcttcg tccactttgc
    54301 cttctaattc tttcaatgtt ttgtccacag agaatagcaa tgcatctaca tcattacgta
    54361 ggtcaacttc ctctttacga gctttatctg cttctgcatt tgcttctgca tcttttacca
    54421 tacgttcgat ttcttcgtct gttagacctg aagaagattt gatcgtgatt ttttgttctt
    54481 tttgtgtacc tagatctttc gcagatacat ttacaatccc gtttttatca atatcgaatg
    54541 ttacttcgat ttgtggaata ccacgaggag ctgctggaat atctgtcaat tggaatcttc
    54601 ctagagtttt gttgtcagca gccattgggc gttcaccttg taatacatgg atgtctactg
    54661 ctggttgatt atctgccgca gttgagaata cttgtgattt acttgttgga atcgttgtat
    54721 tgcggtcaat caatttagtg aacactccac ccattgtttc gatacctaat gacaatggtg
    54781 taacgtcaag taaaacaaca tctttcacat caccagtgat cacgccacct tggatagcag
    54841 cacccatagc aactacttca tcagggttaa ctgatttgtt tggttctttt cctgtttctt
    54901 tacgtactgc ttctacaact gcagggatac gagtagatcc accgactaaa ataacttcgt
    54961 cgatttctga ttgtgataaa cctgcatctt ttagcgcttg acgtactggt acttttgtac
    55021 gttctactaa gtcagctgtt aattcatcaa attttgcacg tgtcaatgtc atttccaaat
    55081 gcaatggtcc agattctcct gctgtgataa atggcaagct gatttgtgtg cttgatacgc
    55141 cagataaatc ttttttcgct ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt
    55201 cttgagacaa gtcaatgctg ttttcttttt tgaattctgc taccatgtag tcgatgattt
    55261 tgttatcaaa gtcgtcccca cctagatggt tgtcgccagc tgttgacaat acatcaaaga
    55321 caccgtctcc tagttcaagg atagatacgt caaaagtacc gccaccaagg tcgaatacta
    55381 agattttttc gtcgcgatct gttttatcta aaccataggc aagagcagct gcagttggtt
    55441 cattgacgat acgttctact tctagaccag cgatcttacc agcatctttt gttgcttgac
    55501 gttgtgcatc attaaagtaa gctggcacag tgataactgc tttttcgact ttttcaccta
    55561 aatagtcttc tgcgaatcct ttgatatatt gaaggatcat tgctgagatt tcttgtggtg
    55621 tatatgattt accttctaca tctactttgt aacctgcttc accgatatgg cgtttgattg
    55681 atgaaatcgt atttgggttt gtcacagctt gacgttttgc aacttcccct acttggattt
    55741 ctccattttt gaatgataca acagaaggtg ttgtacggtt tccttctggg ttagcgatga
    55801 tttttgcttc gtttccttcc aatactgcta ctgcagagtt agtcgtacct aagtcaatac
    55861 cgataatttt actcataatt aacgtctcct tctttaattg aaagtgtaag ttttatatcc
    55921 gcacaattat tgtgcaacaa tgaccatggt tggtcttaac acgcgatcat gtaatttgta
    55981 gccttcttgt agtacttcta caatcgtatc ggctggtgta tcttcagtcg ctggaacagt
    56041 ttgtactgct tgatgaagat taggatcaaa ggcttctcct ttagcaggga ttttttcaat
    56101 tccttcttct tccagtgcgt tacgcaaact ttccaagacc atttccacgc cttttttcaa
    56161 acctgcccct tgatcatctg acacttctgt ggctaatgca cgttccaggt tgtcaattga
    56221 tggaagtaat tttttggcta aatcttgtga acggtatttt tgtaattgct cacgctcatt
    56281 ttttcctcga tttgccatat ttgcgatttc agcacgagcc cgcaagtatt tatcttccat
    56341 ttcttctaac tcagctttta atttcgctgt ttcgaattct tccgcttcag cttctgaaat
    56401 acccgaatct tctgcagctt cgacatcgat ttccggttct ggctgagcat ctgtcatttc
    56461 tttatctagt tcttcttgat tttctttcac gatacagtct tcctttcatt attcagtcag
    56521 ctgtttgatt gctcttacta agaaagagag aacgatcagc tgcaaatagc ttcgttattc
    56581 acattacgag tttgacaagt caagcgaacg gtagtaatca gcaagtttag ctgctagttc
    56641 tcttcgataa acatcgacta gaccgaacat ccgagaataa ggcatactag taggccctaa
    56701 caaggctatt gtacctcttc catgaccatc tatctcgtaa cttgcttgaa tcatactcat
    56761 attctgcaat aaatcattgc ctaattcagc accaatcttg atatgaatat tgctatccat
    56821 cggagtgatc atttgtgtaa gtccgtctga gtctttcata aaagaaaaca tcgatttgaa
    56881 ctgatcaaga tcttgacggg gctcataatc caagagattc atttgtccac tgacataaac
    56941 cttatcttca aaaacatgac caagaacggt gtcaaataac tgagacatcc cttctgtcgt
    57001 ttggaaatat ttatgaagaa tcatcggaat ctccgtacga agtttatggt acaccgtcat
    57061 cagaggatct ccgactagac ggtcgtttac aatctgtacc attttttcta gttcctgact
    57121 tcccatatat tcgggaagag tgaacacttg gctttctaca ttccccatat ctgttacgac
    57181 gatagcaata atctgtcgat tattcaaggg tacgatacga aagccagtca gtcggcggtc
    57241 ttttatttca ggtcccaatg aaaaagtcgt atagcttgtc agctcagata atatctcagc
    57301 agattgctta ataatctcat tgatttcatg aaattctctg cctagagact gacgaatcag
    57361 cagcaaatca tcttctgcta cgcgagcagg ttttaacaaa tgatccacgt aataacgata
    57421 gcctaaagca gatggaatcc gcccagaaga agagtgagtc ttcaacagca atccttcatc
    57481 ttctaatgct ttcatatcat tgcgaatcgt tgctgagctg gccttgactc cctcagacat
    57541 caatgttttg gaaccaacgg gcacgccact actcgtatag tgttggatga tcagacgtaa
    57601 aatgtcgttt tgtcgctttg ttaacatctt cctcacctct cattagcact catatattct
    57661 aagtgctaat ccacttataa atatagcaag aagcccttct cctgtcaaga gaaagactcc
    57721 attttttagc actctaaaca caagagtgct aattctaaat aattgatgta aatccagtta
    57781 tatcaacgtt tcaatagtcc tttattgtat ttttgaactt tttttattta aattcagata
    57841 aaaaacaaat attctttaaa ctatttttca aacaaaaagc gctcaaacac attgttcccc
    57901 aaaaaggtcc ctcttggtgt caaataaaca cggtcttctt cattaacaag taacccttct
    57961 tgctctaatt cccttagcac atcaccgtac accgattcaa ttgttttttg atagcgatca
    58021 tagaaatgtt ttttagtaat ccctgttttt tttcttagtc ctaagaacat ttcttcttct
    58081 atttgatttt ttcgtgtcaa tttttcttct tccaataccg ggcgttcatt tgctcgtaat
    58141 gggcgtaaat agtgttggat tggtcctttg ttgcggtaac gaatatctcc aagataaccg
    58201 ctagcaccag ctccaaaccc aaaatagtgg tcattgttcc agtacatgag gttatgttga
    58261 gattcatgtc cggagagccc aaagttactt atttcatatt ggtgacgtcc atgcttttcc
    58321 attgcttcaa tcgtctcttc aaacattctg gtttccgttt cctgatcagg cagttcgagt
    58381 cttccttgtc gtacccagtt catgaacatt gttttgtttt cgaggatcaa tgagtacaaa
    58441 gaatagtgag gcagatccaa agccaaagct ttttccaaag tatccagata tccttctagt
    58501 gtttgtcccg gtaatgcata aatcaaatcg atgcttacat tggtaaaatt ttctgcttct
    58561 aaaaattgca tagtttgata cacatcttct gcggtgtgtt tacgtccaat ttttttcaat
    58621 aagcgattat caaatgtctg cactcccatg gataaacgat tgacgccata gtttttcatt
    58681 acttgaagtt tttcacgggt caaatcacct ggattcgctt ctacagtaaa ctcattacca
    58741 ttttgaaaag gcaggatctc tctagcacct gataacagtc gatcaagttg tttagcagat
    58801 aaagaagttg gtgtcccgcc ccctacgtaa agtgtttcta aattcgctgt cggatgactt
    58861 tctagcatta ttttcatttc attcagcagc atttccacat actcatctac tggctgccct
    58921 tctaaaaaaa ctttattaaa atcacagtaa tagcagatat gctcgcaaaa tgggatatga
    58981 atgtacgcag aaaccttgtt ttctgcggga tttattgttt ctatcattga aattctttct
    59041 ccttacgtcg aaaagtcgtg cctgttttcg accttcttaa aatcgcgttc aagcaccaac
    59101 tcattgaaat aagccgcata tttagaatca aggaaaccta gctctaaaaa tgcttgctta
    59161 ttgtcatcgg agctacacgg tgcttttaca gcctctcgat aaacggtaat cgcaaagctg
    59221 accttcggca ttaagtcaaa atctgcaaaa atatgaaaag ctattttcac agattcctcc
    59281 ttaagtgctc agaccacaac gctttgctta caacctctca tatacgcata ctaaacgtat
    59341 ttggcgaaca aatccactaa aaaatccaag aatcgacttc ttggatgatt ttttctttca
    59401 ttaaactggt tacctagtta tatatctcta gggttgtttt catgtctgag tgcccgatat
    59461 gatttatttt tcgtttctat aattactttt tgccttccag tttcttttaa tattgtactc
    59521 aagcaagtgg gaattggttc ttttgtcatc catgtatgca aaatgttata atgctggtaa
    59581 acaaaaatga ttacctgtca tttttattta gttcgtattt aattaagttg actatctgta
    59641 tactctgtct aaaacttaag aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg
    59701 tctgtaggtt aaaactgttg tccttgttat agatagtaaa ggagagcttg aaaatgaaaa
    59761 ctgcgattac gtttctatta gaggaatatg ccgattggga aggtgcttat attactagca
    59821 cactaaacca aaatgagaaa tggcaaaaca aaactgcttc tatccaatca gaggtcactt
    59881 ccattggagg attaaaaaca aaaatcgatt acttaatcga acaaatacct gaaaaagttg
    59941 acctttttat tatgatcggt ggcaattcat gggacataaa aaatcatcat ttatatcaaa
    60001 taatcgataa attgttaaaa acagacgtac cagtagctgc tatttgtggg gcagttgatt
    60061 atttagctca gagcggatta ttgcaaggtt acaaacacac cggaaatgcg caatttcctt
    60121 ggttagaatc ggaaaattat cagaataaag aagattttat agaacaacaa gctgtgaaag
    60181 atcgtaattt gattactgcg aatggaacag ctcctataga attcacagac ttgattcttc
    60241 aagcagtcca ttttgaaaca gaagaaataa gagaaaaaac gattcgtcta tacaaacttg
    60301 gattctatga ttactataaa aagtacggag atcctttctc ctaatcattc tcttcaatta
    60361 aatactcctt atcaatagtg tgacaagtaa ttttactgat gattatttgt cacacttttt
    60421 ctgacattta tcaaagaaga tacactctat ttatattcat agacaaggtt ttacggattc
    60481 aaaaattcag gattatgcta tactggtatt aaaaaaataa tgaggtattt tatatgaaaa
    60541 agttaggttt tattttattg ttggtctctt tattccttgt aggatgtacg tctcctagta
    60601 aagaaaatag tgatccatct gcgaccacag acaaaagttc aatcagacaa accagttcag
    60661 ctgaaaaatc aagtactcag aaatcagctt ctactgaaac gaatacatct tcaacaagca
    60721 gtaaagtctc agagcccaat acttcttcta catctagtag tacagttgag acaaatattt
    60781 cttcttcaac aatagacaca acctcctcac agtcttctca gtcaatagat ccgttagcag
    60841 gatattctga tctacaaatt gagtacgcac gtgtttggct agctattaga ggtaatactt
    60901 ttagagatac atcgggtgat ttcgaattac atgttattca ttatcctgca ggaacaccta
    60961 tcgcccctta tgatgcaggg agtgcagtct atccgaaaga tgtggttatg ctcactggga
    61021 aatacagtta ccagggagtt ttagtgtatt caagtaacca tgacgggaca atcactagtt
    61081 atccagttcc ttcacattgg caaatgcctg cagatcaaac atcttatcca gcgttcatta
    61141 gagaaacaac tcaagaaatc attgataatg catctgtagt agctattcct acaggaaatc
    61201 ccaatgacgt gaaacagtta atcgatgtga cagttataga taattagact ttctaatatg
    61261 ttatacaaaa agaagctgac agcattttgt cagcttcttt cactttcttt tctatttaga
    61321 tttattggtc tttgatagac tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa
    61381 acagtcgctt ttctatcttc taccgctact tctgcatagg caccattaat caatgtcagt
    61441 tgtggcggta catgaccaca atcaatatca tacaagatcg gtagttttaa ttcctttgac
    61501 agatcttcgt atacatcctt cattttataa tctaacaaat cctctgccgc acttctaccg
    61561 aacatgatcc ccgagctgcc atcaaaccag cctgccagtt tcatctgaac aagtgatctt
    61621 cttaaatcaa ctgacgatag ctcacagttt tctagaaacc acaagatcgg ctttccatca
    61681 ttatactttc ttttaaaaga ttggacatct ccataaggag taccgattaa atgcctgatc
    61741 gtatcaatac aaccacctaa taatcgtccc ttcatcctta ctgcatctcc gctgactgat
    61801 tgccaatttg ttggttcagt gagatggaaa acagaagatg atgaatgctc atggggccac
    61861 actttttgat acttttcaga agattgctgt agaatcgatt ctccttctct tgttttcagt
    61921 acactttccc acattgctgt cgtttcatcc aagtactctc ctcttaaatc aataaggtta
    61981 gttccatgtg cagtggctat ccctgttttt aatgtgacag caagtagcaa cgtactaata
    62041 tctgaataac ctactatcca ttttggttgt agagcatcaa aatcgacaaa atcgagaatc
    62101 tcaatcaata attctcctcc ccaagtcgga aagatcattt ggatattttc atcttgcatc
    62161 atttgattga attcatctgc tcgttttctt gctggacctg atttttcctt atcttgcgtc
    62221 caaagcgtct ttccagtaac tacttggaaa gatttttctt ccattctctt taaagctgat
    62281 tcgagtaacg aatggtgttc ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt
    62341 gcatttcttt ctaataatgg ataacttatc atgttgcttc acctccaagt atgcactatt
    62401 ttacttggtt aattgttttt ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc
    62461 tggatgattt tgtttaagtc aaccgtttgg atgaccatcg tttgatctgg tctacctggt
    62521 gcaaaaacaa gtttttcatt tgataagagc tcttcatcaa taataatcgg aatctgtttt
    62581 tcataaccaa acggagaaac agcgccctgc ttttgtcccg tttgttcttc catgcgttct
    62641 gatgaaacaa tagaaagcct tttaccgacg atcttcccta atgctttgaa atccgtttgt
    62701 ttcgtagtaa aagtcagata tacgtagtta ttatcttttt tatcttttaa gaataagctt
    62761 ttggtctctg tacctgtaaa tccttgttct ttctttatta ccaaagcatc ttcatttgta
    62821 taaatggctc gatgatgata caactcaaaa tatatatttt cgtgtaagaa tttctttgcc
    62881 aagtcaatca tttttatgtc tcttttctat tttattattt gagacatctt atcatataaa
    62941 tatagcggtt acaacattac ttgtgttttg ttcgtttttt atacataact attgtttttc
    63001 tggtaaaata aatcaaacca cttaatggag gcgttttaga atggaaaaat ttcagccgat
    63061 catcaatcaa atcaaagaaa aagaacatca acaaaagatc aaagaccttt ttgctaagat
    63121 aacagaaacg tatccccagc tagacacaac tgtaaaatgg aaccaaccta tgtttactga
    63181 ccatggtacc tttattattg catttagttt tgctaaaaat catttttcta tcgcaccgga
    63241 aaaagcagcg attcgtgccc ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga
    63301 taacgtcatc aaagttcctt ggaaaagtgt aatcaactgg gaattgattg aacaattgat
    63361 ttcatttaat attgaagata aaaaaggtca tgataaattt tggcgataaa gctttatacg
    63421 aataaacctt ttactggcga ggaaactatt catcacctta tacaggtttt gttcaatcct
    63481 atccttttta tcatccctgg ttaaacactt aattgacaaa gtatctgcta tttttataag
    63541 gagtctttct tgtgctgaaa aaaatcactg attgaatggc tttgaatttt ctacttagta
    63601 aaattatttt ttcagcaatt ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa
    63661 tatcagtgat ctttcataat actgcccatt attttcaggt atatttaaat ataatatcac
    63721 tttaggagag aacgaatttc tttagagaaa taaaagttag aattttctga cttgattttt
    63781 acaatgacct taattaaaat cgttctttct acatttcgtg attaagcaaa tatcaactaa
    63841 gtcagtagaa ccattcacat aggagggtga acattgaaag atttttactc agtaaatgaa
    63901 ttagccgaac aattaggtgt tactacacgt tctatcagaa attatcttca tgaaggaaaa
    63961 ctaaaaggaa caaaggttgg cgggcaatgg aaattttctg aacgtaatct ttttgagttc
    64021 ctatacggag atcaagcaga ggaagcagca aaagaaatgc agcgattcat gctaaatgca
    64081 ccaatcacga tgcgtttcaa ccttcaatat cgtgatttta cagcaatcaa tcaattccga
    64141 gaacaattag tccagtacca taacgatgtt tacgcaaata aaaaagaccg tcttttacaa
    64201 tacgatcttt ataaagacaa tcatgcggag atactaatcg gtggtaactt caattatgtc
    64261 gtaaacttta gtcaatggat caatgaaaaa ttattgatcc agacagatat ttcccttgta
    64321 tcttaaacaa ttaaaaagcg ctagaaattc cagagatgtt ggaaattcta gcgctatttt
    64381 tttataagta cttactgttt ggttcaaaca aatatttttt taataaatga aattttacca
    64441 tctttatcct caaataaaga ccgtgaccat gcttcctact acgatcagca ataatccgat
    64501 aatcgttaat tttttttctt ttggtgtctt attttcatgc aggatgtaca taccgcctaa
    64561 tgtagagata actacgttta gctgtgtaat gatatatgca gtagcaaccc cattttcatt
    64621 tgctgaaaaa atataagcca atgctgacac actaaagatg gctccaacga tcgttgcttt
    64681 ccaactcttg tcttcagaaa atgcggttgg tttacggaaa atcacataaa tcactgcccc
    64741 taaaaagaca ccgatcattt gcggaaagaa aatggaaagt ccgcttgcat caactagttt
    64801 aggcaatgca ctgtataccc aataaccaat acttgttgat gctaatagac taattccttt
    64861 tgtgataccg cctttttttc caggatcatc actgattgcc gttaaagcag aaccaaatac
    64921 aagaagaatg attgctgctg ctccaatgat tttccccata cttccagacc attctccaaa
    64981 agcgaagaca ccaatcaacg aggttccgac taactgtaat gcagtcgaaa ttggcatcgt
    65041 tttagaaaca ccaatcaagt tgaaagatgt atactgtcct gtttgtccaa taacccaaaa
    65101 tgcaccagaa agtaaactaa tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa
    65161 taattgaacg agtaagccga caagcaccgc tccaatacca gttcctaata tctcatttgt
    65221 tggtctgcca ccaatctttt tcaaaatcag tggctggatc ccccagccaa ctgccgggat
    65281 caatgcaatc aataaaccca tttttattca cccttatcct tttccacctt ggaaagaagg
    65341 atataaagtc atcccaccat ctgcaaatag tgtaatacct gtaacataac tggattcgtc
    65401 tgaagccaac catgcagctg tcgccgctat ttcttttggt tcacctacgc gtcctagtgg
    65461 gatcatactt tccaagagtt ctttttgttc aggatcatca aattttgccg cattaatagg
    65521 tgtacggata gcaccagggc ctatactatt gattctgatt cccttcggtg catactccat
    65581 tgctaccgtt tcggtaaata attttacccc gccttttgaa gcagcgtaat gagcaaacgt
    65641 tggccatggg atctgttcat gaacagaaga catattgatg atatttcctt ttttattatt
    65701 ctctgtaaag taatttaaag ccgctttcgt tccgataaaa acgccagtca aattgacttt
    65761 taacactctt tcccaatctt caaggctaag ttcatgggtt ggatgctgat tttccatccc
    65821 agcattatta acccaaacgt ccagtgtccc aaaattttct aaagccgtat caagtaaacg
    65881 ctgtattcct tcttcagaac tgacatctgc ttgtacagag actgcttttc cgcctgcctc
    65941 ttcgattgct ttgactgctt tttctgctcc ttctttatcc gagttatagt tgacaactac
    66001 attcattttt tctttcccga atcgttgagc aatcgctgtt ccaatcccct ttgagccacc
    66061 agttactact gcgactttgt tttctaattc cttgtacata gtacccactc ctttttcata
    66121 cctaaacctg ttattacgga taacttttat ttccgcctta aagtgagtgt acaccaagat
    66181 atatagtcta aagcaaatga tacgctctag caatcgtttt catgaattgc tagtgtcctt
    66241 ttatttctat aaagaaagag gttgtgacag aagtggtcag cttcaagaaa tttcggctta
    66301 tttccgaaga agctacttct gaaaaaccat ttatttggaa aaaaaggctg tgacaagact
    66361 tttgtcacag cccctttcct gggcttattt atcacccatt ttattgtaat tgctagttat
    66421 acataaacaa gagattttat gcataatgtt ttattgctga gaaaaatatt cagctgttgc
    66481 ttgttcatcc tccttgagtt gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc
    66541 tcttaaaaat tgattccagc gtaccgcgac atgttcacca tagatatctt gattaaagtc
    66601 caaaatatac aactctacag tcagctcccg tccttcaccg aatgtatcat tatgtccaat
    66661 tgaacccatg gctggatacc atgtttcccc aatttttatt tccgacacat aaacaccttc
    66721 tttaggcaaa tgaaccgtgc tctttacttt gacatttgct gtagggaatc ctagtagtct
    66781 gcctcgtgca tcgccatgga caactacccc ttctacctca tatgtgcaac ctaatagttt
    66841 tgctgcttct tttacttgtc cgctgtctag taatccacga atacgggtag agctgatttt
    66901 ttcaccttgt tcttcttctt tagaaactgt gacgattttg aatcttcctt tggcatattc
    66961 aggaaaatga gcgacatctg caatgtcttt tggtccatac gtataatcaa aaccggaaac
    67021 agcatatttt gcatgtaacc ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg
    67081 agcaaaagaa gaagtaaaat caacttcata taaataatct atacccaaag cggccatttt
    67141 ttcttctttt tgcttaaggc tagtcaaata tttcacgctg ctaggatcga tctttttaaa
    67201 gacaatagat ggatgctgat tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc
    67261 aatttccata cctgtcttta tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc
    67321 cagcactaaa acgacatcgt cttctggaat ttgttctgat tggtaaggat gtctgatctt
    67381 gatgatattc attcgttatt cctcatttct caatacttta ctaggtttta atttgttttt
    67441 ttctttcggg tttggctgat aaatactgac aacttttcct ttataaaaca aagcaatttc
    67501 ttctgacggc atttctggga gcgcaaatac agtataatca agtctcatac cgttttttac
    67561 tttctgccat acttcatcag taatatctac tcgtttgaac cgttccactc ctttttctat
    67621 tggcagcaaa caatcatcga ttgtcccatt tttcacagct tgagctactt cttctaatgt
    67681 cagagcatcc ttctgggaca taccggcact tgctgttcgg gtcagatcag acatgtgcgc
    67741 agggtaacca agtttcttgc ctgtatctac agctaatgtg cggacatagg ttcctttccc
    67801 gcattctact tcgaagcgcc aagaaatggt tcctgtcact tcgtctcttg tcaattcact
    67861 tgtccggata aaacggtaaa tctgagcctt tctaacagga cgctccactg tttcattatt
    67921 gcgggcatat tcatataacc gcttaccgtt gacttttacc gctgagtaca ttggtggtat
    67981 ctgggtgatc tcaccaataa atgaagccat cacttcatct attttttttt cagataaaga
    68041 catatctact ggttttgttt ctacccgttc tccggatcga tcttccgtcg ttgtactgaa
    68101 acctagagtt atctcgccta catagatttt cccagaatct gtcaaatatt cgataacttt
    68161 tgtggctttt ccaatacaaa tgggcaacac accgtctacg tctgggtcaa gcgtaccccc
    68221 atgtccgatt tttttcgtat gtaagatttt tcttaatttg aatacgcaat catgacttgt
    68281 cattccgcgt tctttccata aaggtaataa cccgtccatg tcttttctcc atttcatttt
    68341 tgatttaact gaattattat aacacattgc atcaattact gacgaatttg acatcataat
    68401 gaacggtttt tatttttcgg caataaaaac gtaacaaaat tcatgctcac gtaaatagaa
    68461 atcatgttat gcttttttaa aggagggaat agaatggaag attttttctt tgcaccattt
    68521 gattggattt atgggtggct tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa
    68581 aaaaagactg gccattcttc cagtcttttt tccgtttttt ttatttattt tttcagtttc
    68641 ttaatcaatg taaaaaattc tattttgcgg gatcgatttt atcattttat tggtcaagat
    68701 ttttccattt ccaatccaat acttctggca gatcaatacc attttcacga atatagtttt
    68761 gatgttcttg taatttctga tccatttttt tcgcaaaatc ttctgcttga cttcctttga
    68821 tttttaaagc tgcgtctttt gccaaatgat aacgatcaag ttcatttact acacgcatgt
    68881 caaatggtgt cgtgatatct ccattttctt tgtagccatg aatcattaaa tgatggttat
    68941 gacgatcaaa gaaaatcgtg cggaccaatg tttcataacc gtggaaagca aataagatcg
    69001 gtttgtcttt tgtaaaatat tgatcaaatt cttcatcgct caacccacgc ggatcttgac
    69061 ttggatgacg taatttcaat aaatctacga cattgatgaa ccgaattttt agttttggga
    69121 attcttccag cagcaaagtt acagctgcta atgcttctaa gttagattcc gttcctgcag
    69181 cagcaatgac caattcaggt tcctcctctt ttgctgtcga agcccaatca atgattttca
    69241 agccttcatt cactaagacg gctgcttctt ctgctgagta aaattgagca cgcggatgtt
    69301 tggaagagat gatcaaattg attttttctg acgagcggaa tgctttatcc atgactgcga
    69361 gcaatgtatt tgcatccgca ggcagatatt cacggatgaa ctccgccttc ttttctgcca
    69421 aatgtgtcaa tattcctgga tcttggtggc tatatccatt gtgatcttgc tggaagactg
    69481 ttgagcttgc aatgagattt agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt
    69541 tccgcatcca tttgaagtgc tgagtcaaca tagagtcaac cactcgcaag aatgattcgt
    69601 aacttgcaaa aaaccatgcc gtccagtcag aacgtaccct tctaagaacc cttcatcttg
    69661 gtgttccgat aattgactgt cgatgacgcg accagatggc gaaagccatt catcattttc
    69721 tggttcgatt ttttccatcc attggcgatt tgtcgttttg aaaacttgat tcaatcggtt
    69781 tgattttgtt tcatctggtc caaatatacg gaaattttct ggatttttct caatgatctc
    69841 gcgaacgaat ttaccaagtt cggtcatatc ttctgcttta acggagcctg gtttttcaaa
    69901 ttgcaaagta tattttttcc aatctggcat aataagcggt cttggatcga ttccaccatt
    69961 ggtgatagga ttttttgcca ttctttggtc accagtaggc gcgatttctc tgatttcttc
    70021 taaaatacgt ccttgtgcat caaataattt ttctggttca taggatttca gccaacggag
    70081 taaagcatct gcatgatcca tatgttcttg atctacagga atcggtactt ggtgtgctcg
    70141 gaaggttcct tcaattggtt ccccgtccca ttcttttgga ccggtccatc cttttggtgt
    70201 gcgtacgatc aatactggcc attttggcat cacgacatct gttgctgtct tcaggcgagc
    70261 ttctttttgg atggatttaa ttttctcgat tgcttgatcc attttttcag ccataacagg
    70321 atttaatttt tctgaatcat ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa
    70381 gtaatttgct aattctccat ctgatttacg tgccaagatc gttgggttgg ctatttttgc
    70441 accatttaaa tgaaggatcg gtaaaacagc tccatctatc actggattga taaagacatt
    70501 tgagaaccag cttccagcta aaggaccggt ttctgcttca ccatcaccaa tcacgacagc
    70561 agagattact tcaggattgt ctaatacagc tccgactgca tgtgaaagag agtatccaag
    70621 ctctccgcct tcatggatag aacctggtgt ttctggtgct gcatgagaag caatgcctcc
    70681 aggaaatgaa aatcgtttga ataatttttg cattcctgtc tcatcttccg ttacttctgg
    70741 atagatctct gtgtaacttc catctaaata agcattcgat accatgactt gtccgccgtg
    70801 tcctggccct tcaatataaa acatgttcaa atcatattta ttgatgactc ggttcaaatg
    70861 gacataaata aaattttggc caggaatcgt tccccaatga ccgattggat gttttttgac
    70921 atcttcgggt ttcagtgtac gtctcagtaa ggggttatct ttaagataaa gttgcccaac
    70981 agaaagataa ttagcagcac gccaccaagc cgtcatttta tcaaagtatt ctttactaga
    71041 ataatccata cataacactc cttttcatta ttactcttat tctagcgcag atactcccaa
    71101 ttgaaaccta ataagcactt ctgttcattt gtttggattt tatatctagc caaatgaaca
    71161 ggtataaaaa accacgaaat ttttcctttc gctgacgttt tttacattga tccaaaaaac
    71221 ttgtcgttat cacaatggct gttccgcagt tcatttcgct cctcatcatg cataatttgc
    71281 tgaatgacgc tgggccagtt aacacattgc tggaaaactg gggctggatc gagcaattcc
    71341 tttcctgatt gatccgtttt atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg
    71401 tatcccagct actatgctaa tttctacagg gattatccaa aatttggatc gtgaccaaat
    71461 cgaagcggcg taatcggaaa tatcaataac ttcaacgtga tttttctttt aactgtcaac
    71521 ggaccgacaa actcaaactt ttatggtgca ggttccactg atcttctagt tacttggcta
    71581 tacctgactg tcactaaaat agattacaat ctagcttccg ttatcggtat cttgatcttt
    71641 atctatatgc aaaaatttat atactactag aaaaaagaaa gtcgaatttt ttacttttct
    71701 ttttctcttt tttggttagc tgacacgccg caagactaaa gtcatggaac tcttaggtaa
    71761 tacgtcttaa tgaaaacaaa aaaatcccgc aacccct
//
LOCUS       DACGLH010000008        69537 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.009, whole genome shotgun sequence.
ACCESSION   DACGLH010000008 DACGLH010000000
VERSION     DACGLH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.009"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            261..941
                     /locus_tag="IXT21_000555"
     CDS             261..941
                     /locus_tag="IXT21_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ4867987.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAATGTFTADLTW
                     NLVAGPVA"
     gene            1031..2065
                     /locus_tag="IXT21_000556"
     CDS             1031..2065
                     /locus_tag="IXT21_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ4867988.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            2079..2414
                     /locus_tag="IXT21_000557"
                     /pseudo
     CDS             2079..2414
                     /locus_tag="IXT21_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2398..4878
                     /locus_tag="IXT21_000558"
     CDS             2398..4878
                     /locus_tag="IXT21_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4867989.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4934..6493
                     /locus_tag="IXT21_000559"
     CDS             4934..6493
                     /locus_tag="IXT21_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4867990.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6490..7854
                     /locus_tag="IXT21_000560"
     CDS             6490..7854
                     /locus_tag="IXT21_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4867991.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            8025..9095
                     /locus_tag="IXT21_000561"
     CDS             8025..9095
                     /locus_tag="IXT21_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4867992.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9110..9778
                     /locus_tag="IXT21_000562"
     CDS             9110..9778
                     /locus_tag="IXT21_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4867993.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9798..10376
                     /locus_tag="IXT21_000563"
     CDS             9798..10376
                     /locus_tag="IXT21_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4867994.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10779..11690
                     /locus_tag="IXT21_000564"
     CDS             10779..11690
                     /locus_tag="IXT21_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4867995.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11946..12863)
                     /locus_tag="IXT21_000565"
     CDS             complement(11946..12863)
                     /locus_tag="IXT21_000565"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ4867996.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13065..13502
                     /gene="zapA"
                     /locus_tag="IXT21_000566"
     CDS             13065..13502
                     /gene="zapA"
                     /locus_tag="IXT21_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ4867997.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13560..14108
                     /locus_tag="IXT21_000567"
     CDS             13560..14108
                     /locus_tag="IXT21_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ4867998.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14223..16583
                     /locus_tag="IXT21_000568"
     CDS             14223..16583
                     /locus_tag="IXT21_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ4867999.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16670..16984
                     /gene="trxA"
                     /locus_tag="IXT21_000569"
     CDS             16670..16984
                     /gene="trxA"
                     /locus_tag="IXT21_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ4868000.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17166..18944
                     /gene="uvrC"
                     /locus_tag="IXT21_000570"
     CDS             17166..18944
                     /gene="uvrC"
                     /locus_tag="IXT21_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ4868001.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19089..19988
                     /locus_tag="IXT21_000571"
     CDS             19089..19988
                     /locus_tag="IXT21_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868002.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19981..21243
                     /locus_tag="IXT21_000572"
     CDS             19981..21243
                     /locus_tag="IXT21_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868003.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21406..23304
                     /locus_tag="IXT21_000573"
     CDS             21406..23304
                     /locus_tag="IXT21_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ4868004.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(23603..24322)
                     /locus_tag="IXT21_000574"
     CDS             complement(23603..24322)
                     /locus_tag="IXT21_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ4868005.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            24659..24991
                     /locus_tag="IXT21_000575"
     CDS             24659..24991
                     /locus_tag="IXT21_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868006.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24978..26981
                     /locus_tag="IXT21_000576"
     CDS             24978..26981
                     /locus_tag="IXT21_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ4868007.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27000..27470
                     /locus_tag="IXT21_000577"
     CDS             27000..27470
                     /locus_tag="IXT21_000577"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ4868008.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27491..28075
                     /locus_tag="IXT21_000578"
     CDS             27491..28075
                     /locus_tag="IXT21_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ4868009.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28090..29076
                     /locus_tag="IXT21_000579"
     CDS             28090..29076
                     /locus_tag="IXT21_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ4868010.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            29066..29377
                     /locus_tag="IXT21_000580"
     CDS             29066..29377
                     /locus_tag="IXT21_000580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ4868011.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29399..31180
                     /locus_tag="IXT21_000581"
     CDS             29399..31180
                     /locus_tag="IXT21_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ4868012.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31173..32549
                     /locus_tag="IXT21_000582"
     CDS             31173..32549
                     /locus_tag="IXT21_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ4868013.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32556..33191
                     /locus_tag="IXT21_000583"
     CDS             32556..33191
                     /locus_tag="IXT21_000583"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ4868014.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33221..34576
                     /locus_tag="IXT21_000584"
     CDS             33221..34576
                     /locus_tag="IXT21_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ4868015.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34768..36162)
                     /locus_tag="IXT21_000585"
     CDS             complement(34768..36162)
                     /locus_tag="IXT21_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ4868016.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36254..37225
                     /locus_tag="IXT21_000586"
     CDS             36254..37225
                     /locus_tag="IXT21_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ4868017.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37222..38004
                     /locus_tag="IXT21_000587"
     CDS             37222..38004
                     /locus_tag="IXT21_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ4868018.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38173..38544)
                     /locus_tag="IXT21_000588"
     CDS             complement(38173..38544)
                     /locus_tag="IXT21_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ4868019.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38678..39121
                     /locus_tag="IXT21_000589"
     CDS             38678..39121
                     /locus_tag="IXT21_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ4868020.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39164..39973)
                     /gene="yidA"
                     /locus_tag="IXT21_000590"
     CDS             complement(39164..39973)
                     /gene="yidA"
                     /locus_tag="IXT21_000590"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ4868021.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(39992..41359)
                     /locus_tag="IXT21_000591"
     CDS             complement(39992..41359)
                     /locus_tag="IXT21_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4868022.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41343..42173)
                     /locus_tag="IXT21_000592"
     CDS             complement(41343..42173)
                     /locus_tag="IXT21_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ4868023.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42336..42773
                     /locus_tag="IXT21_000593"
     CDS             42336..42773
                     /locus_tag="IXT21_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ4868024.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42849..43469
                     /locus_tag="IXT21_000594"
     CDS             42849..43469
                     /locus_tag="IXT21_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ4868025.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43658..44164
                     /locus_tag="IXT21_000595"
     CDS             43658..44164
                     /locus_tag="IXT21_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ4868026.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44181..45080
                     /locus_tag="IXT21_000596"
     CDS             44181..45080
                     /locus_tag="IXT21_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ4868027.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45167..46051
                     /locus_tag="IXT21_000597"
     CDS             45167..46051
                     /locus_tag="IXT21_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ4868028.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46243..46314)
                     /locus_tag="IXT21_000598"
     tRNA            complement(46243..46314)
                     /locus_tag="IXT21_000598"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46279..46281),aa:Arg,seq:ccg)
     gene            46374..47708
                     /gene="rpoN"
                     /locus_tag="IXT21_000599"
     CDS             46374..47708
                     /gene="rpoN"
                     /locus_tag="IXT21_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ4868029.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47911..48948
                     /locus_tag="IXT21_000600"
     CDS             47911..48948
                     /locus_tag="IXT21_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ4868030.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            48989..49990
                     /gene="gap"
                     /locus_tag="IXT21_000601"
     CDS             48989..49990
                     /gene="gap"
                     /locus_tag="IXT21_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ4868031.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50100..51290
                     /locus_tag="IXT21_000602"
     CDS             50100..51290
                     /locus_tag="IXT21_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ4868032.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51394..52149
                     /locus_tag="IXT21_000603"
     CDS             51394..52149
                     /locus_tag="IXT21_000603"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ4868033.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52265..53563
                     /gene="eno"
                     /locus_tag="IXT21_000604"
     CDS             52265..53563
                     /gene="eno"
                     /locus_tag="IXT21_000604"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ4868034.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            53963..55102
                     /locus_tag="IXT21_000605"
     CDS             53963..55102
                     /locus_tag="IXT21_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ4868035.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(55169..55744)
                     /locus_tag="IXT21_000606"
     CDS             complement(55169..55744)
                     /locus_tag="IXT21_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4868036.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(55749..56723)
                     /locus_tag="IXT21_000607"
     CDS             complement(55749..56723)
                     /locus_tag="IXT21_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ4868037.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(56747..56791)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            57006..57974
                     /locus_tag="IXT21_000608"
     CDS             57006..57974
                     /locus_tag="IXT21_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4868038.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            58087..58365
                     /locus_tag="IXT21_000609"
     CDS             58087..58365
                     /locus_tag="IXT21_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ4868039.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            58483..58719
                     /gene="secG"
                     /locus_tag="IXT21_000610"
     CDS             58483..58719
                     /gene="secG"
                     /locus_tag="IXT21_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ4868040.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            58831..59577
                     /locus_tag="IXT21_000611"
     CDS             58831..59577
                     /locus_tag="IXT21_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4868041.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            59580..61937
                     /gene="rnr"
                     /locus_tag="IXT21_000612"
     CDS             59580..61937
                     /gene="rnr"
                     /locus_tag="IXT21_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ4868042.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            61959..62423
                     /gene="smpB"
                     /locus_tag="IXT21_000613"
     CDS             61959..62423
                     /gene="smpB"
                     /locus_tag="IXT21_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ4868043.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            62725..63444
                     /gene="atpB"
                     /locus_tag="IXT21_000614"
     CDS             62725..63444
                     /gene="atpB"
                     /locus_tag="IXT21_000614"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ4868044.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            63497..63712
                     /gene="atpE"
                     /locus_tag="IXT21_000615"
     CDS             63497..63712
                     /gene="atpE"
                     /locus_tag="IXT21_000615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ4868045.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            63803..64327
                     /gene="atpF"
                     /locus_tag="IXT21_000616"
     CDS             63803..64327
                     /gene="atpF"
                     /locus_tag="IXT21_000616"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ4868046.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            64314..64856
                     /locus_tag="IXT21_000617"
     CDS             64314..64856
                     /locus_tag="IXT21_000617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ4868047.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            64879..66435
                     /locus_tag="IXT21_000618"
     CDS             64879..66435
                     /locus_tag="IXT21_000618"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ4868048.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            66449..67351
                     /locus_tag="IXT21_000619"
     CDS             66449..67351
                     /locus_tag="IXT21_000619"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ4868049.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            67374..68780
                     /gene="atpD"
                     /locus_tag="IXT21_000620"
     CDS             67374..68780
                     /gene="atpD"
                     /locus_tag="IXT21_000620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ4868050.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            68794..69216
                     /locus_tag="IXT21_000621"
     CDS             68794..69216
                     /locus_tag="IXT21_000621"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ4868051.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        23081 a        12200 c        14762 g        19494 t
ORIGIN      
        1 gagtgcggca gatgatgccg tactcctatt tttatagttc aaggatgtca ctaatagaat
       61 aatttgaaac gaaaaaataa aattgaaata ttttttcata cttaataaaa aaggaagaat
      121 attcatgtaa acgattttat tgtttttttc tcttttatac aaaaagggtc ataatttcaa
      181 gtaagcggat gaattcatga ggattttttg catactgtct ctactatact taggtcacta
      241 agaaaaagga gtgatttatt atgaaaaaga acatgattgg gacattatta ctttcaacac
      301 ttcttctagg cggcttggca acaccagctt ttgctgaggg tcaagcgact tcaaaaggag
      361 atattacctt tactgaacca acaaataccg ttgagcctct taaccctact gatccttcaa
      421 aaccagtaga gccagcagat ccggaaaatc cagcaactgg ccagacaggt tctttaacat
      481 tggatgtagt tcctgaattg ccatttggta cacacgagat tgaaagcgga acaaaaacgt
      541 atcaagtaga cgcttcaaaa aacgatacac cttatctcca agtatcagat cgtcgaggtg
      601 taggtgcaga tggccaagca caagggtgga acgtgacagt ttcggtttca gattttgtaa
      661 acggttcaca agttttgcaa ggcgctgaat tagattttgg aacatccact gttaaaagta
      721 cgtcagataa cgaatccact ggaccaacta gtcaaactgt aactgggttg agtaaagcaa
      781 gtgcagcgac accaatattt acagcagcaa aagatcaagg tctgggaaca tggctttctg
      841 tttatgatcc agcaaatatc acactgaaag tgccgaaagc agcaactgga acattcacgg
      901 ctgatttgac ttggaacctc gtggctggcc cagtagcata gagtatgaaa taaattcaac
      961 aaactgcggt gaaaatacta taggtgaagg attttttacc gcagttgttg ttgaaaggat
     1021 gtggtggaca atgaatcaaa gtaaacgact gtttgtttcg tttttttcta ttttaagtct
     1081 atttttcatt tttccgtcaa tcagccaagc aaaggattca gcaggagact ttggaatcaa
     1141 accggtattt ccagagaacc aaatagataa agcaatcggt tattttgatt tattagtggc
     1201 accggaacaa aatcagacac tagaagtaat tatcagcaat tcttcagatg aagaacgaac
     1261 atttgaggtc tctgtcaatc cggcggtaac gagtgatggt ggaacgatcg attatagtca
     1321 aaaaaaccca acgttagatg agacattgcc gtttgatgtc cgtgacgttt tgctcattgc
     1381 gaaaaaggaa atcaatgtat cagcacatgc agaaacaact atacctatag aagtgaaaat
     1441 cccagcaaaa agctttaagg gacgtgtatt agcgggtatt catgtttcac ctaaagaaga
     1501 agctgaaaca gaaaatgcta cagaaggagc acaaatcaaa aatcgtattg cttataatct
     1561 cgcagtcgtt ttacaagaaa gtcaagagac gatagaaccg gatctgaaac ttttatcagg
     1621 agatttggac gaggtcaacg caaagccaac tgttcagctt cgtttccaaa atcctcagcc
     1681 taggattatc agcaatttga tatttacgag caagatcttt tatgaaaatc agctttatat
     1741 tgaaaatacg tctaatgaat ttttagttgc accaaattcg aattttcact tgaatcttga
     1801 tttagctggt gataaagcaa aagccggcga ttaccgcgca gaaatcatag caaaatcagg
     1861 cgacagtaat gaatggcgat ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa
     1921 agtaaatgaa aattccgttt ttgcagtaca agaacaatct ttcccatggc tttatgcagg
     1981 attggttgct gcagttctag tgttatggtt gattttgctg tttttgtggg tccacaagaa
     2041 aaagaaaaaa ggagaaacaa catgatgagg tgatttcgtt gaaaacatcg atcttattgg
     2101 gagggattat tgcaagtttg ctgttcttca gctggcaaga gccagtccaa gcagatacca
     2161 aaagttcagg tacgattaca ttttatgatc taagtccagt tccgcatcca agtggttcag
     2221 tcaatggcgc atcaaaatag cagacacagc taccgattta taaagaatct aatcaacatg
     2281 gcgtattccc ggagacgaat gaagaaaaga tccgttttct ttatttgatc attggtatag
     2341 aattatggct tcttgcgtgg ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg
     2401 ttgctacaaa ataaattttc attttttcgc tccttctttt ttacttcgct ttttgtttta
     2461 agtatattta tcttttcacc ttcggcagaa gctgttggag caacgcctcc tgccatttct
     2521 tatgatccat acacgtatga aaatatcgta cctccaaatg gtggacgtag tgcagtcaat
     2581 ggcgggaagc ttgtccaact aactggggct aaccaattgc ctaaattgac tgctacttat
     2641 ttgaacgatg gttcaaatga tccttggaaa ggtggcacgg tccgttttca ttgggaaagc
     2701 catgtgaaac taacaaatgt acttgatttg gtgaatatca ctgcacaagg atttgcattt
     2761 gtggcgatca tcaatttgcc ggaacatgta tcaagcagtg atgtattaaa agcaattgac
     2821 tggaacaacg cctacttgta tattgccggt gcccccatca aggttactag cggtagttta
     2881 ttatcagcag gaagccatac cttgcgtttt gggatggggt cgtgggatac aaatactcta
     2941 ttggcagata ttattgctat tattacgcga ggcgaattca acgtggacga tatcccgctt
     3001 caatttgatg ttacagctaa tgtcgcgaag atgacagaaa acggagataa atatgatacg
     3061 agtccaaaca aaattttgac caagggtaaa ttacaacctt ctatgtctaa aaaggccgtt
     3121 ttttctgttg atttttatga ttcgaataat attatccaag atcgtatgtt caattcgatt
     3181 ttagctccag ctgcaggtag attttatcct aaattaactg gcggaaactt gaatccagga
     3241 gcatatgcat tttctgcaag tgcagagata aatacgtgga atagttacat ctcaacaagt
     3301 gattcaacag gaaaatataa tacatttgct gataccacag aatacgtgga tggaaataac
     3361 cagaaccttc caggctctac caatagtcgg acattgacga tggggctgac tgaaggacgt
     3421 tttgatcagt tgccatttag tcgctttgat cgcgtagtca actattttac aggaaaaaac
     3481 gttacacaag gagcaatact gactcatact ccagcaagca caagtggatt gaatcagaca
     3541 gccaatatcg tgtacagtgg gaaagatgcg caaggcggat tgctttctcc tgttgcttta
     3601 aaggtattgg agcagtacaa gctagacgga acggttttac caacaaactt aacagccgat
     3661 agtcagtggg gaaagctcct ttcttctgca tcgtaccaag tcacggccaa ttggtcagca
     3721 actgaactaa aaaagggttc gatccattac aggctttatt cgaaaggaac ccaaaaatta
     3781 gcgacaggat ggacagatca acttttccaa acgatccaat caaataacgg gggtgtaaat
     3841 acagctactt ctacactacc tgcattggct tcagggcaat attattttga ttatcgcttg
     3901 gtggacgatg tacttactaa catgtatccg tcttttgcgt acaaatggca gtctgaacaa
     3961 gctggaatca ccactcttcc tcaaataaca gtggctaatt ttccatctgt gagtgcagat
     4021 agtcagctaa aaaaccttag cagacaacct gagacaggag atcccctgtc tgctctatca
     4081 ggagatacga ttcaagaaaa tgtgacattc acgttaacaa aatctgggga tgcgataacc
     4141 gacaaaaaaa tcacgatctc acttcctgaa aatactactt atatcaatgg ttccttaaaa
     4201 ttaaatggta caactatcgc agataccggc attcaacaag gagtttctgt cccagcagat
     4261 ctattgagca aaataggaga taccattcat cttacttaca actatcaatt aaatactgta
     4321 gatacttctg tacaatccgt cagcatactg acaaaggcag ctgtactttc cagcaatatt
     4381 acattagcag atggtgctaa gcttcctaac ccagttgtcc aaacatctgc taaaacaatt
     4441 cttgtcccca aacaagaatt aacattagtt aatgtaccag atgattttac atttggtaac
     4501 gatctcccca aaccgttgaa gacaagttat tatgaagcaa aaggcgattt ttcttttgat
     4561 gtgcgggata ctcggcttcc aagcacgtct ccttggcaac tgacagggac attgacttct
     4621 ttatttaaga atgatcaagg tcaagaatta agcggaacaa aattatactt caatcattct
     4681 ggatcaaaac aattgattca gcaaggtcaa aacacgttga tttatgagag tgatggaaca
     4741 gctaaaggag aggttctcgt tgactttccc gataccgatg gtttactctt agaggtaaat
     4801 agcagtacga atgctcaacc tggcgcaacg tatcaaggca tggtcacatg ggaactgaca
     4861 gctgggccaa ccagctaagt gattagaatt tgtatggatc tgtgataact gtcacagatc
     4921 ctctttttca aggatgagaa ggaaacttct gctgttaaaa tcagaaatgc ttggaggagt
     4981 ggacttggaa cttttcagtt tgcttgaaaa aaatgaacaa cggcaaatca aacttttcca
     5041 acttttatta gaggaaaaag gagagatttc cgttaataac gtacttaccc gcttatcagt
     5101 tgatcgggct accttgaaag aagatctgtt ttatttacaa gctaacttgg aaaatatcaa
     5161 acaattggaa attgttatcc aagaagggaa tattttatta atcagacatg gaaatatagc
     5221 agcagtcgat gtttattatc attgctactt gcgctattct acaaaatatc agatacttct
     5281 ttatctgttg gaaaatggct catttgatcg gcagaaactt gtcatggaac ttaatatcag
     5341 ttcggctact ttgtctcgca gagtgaagga attaaatgat tctttaaatg agtttcatct
     5401 gaaaataaaa agcgggcggc tgaccggtcc agaaagtcaa atttgttatt tttatttcca
     5461 attattttgg tttggacgtc cttatcttga aaacaaaaaa gaatacagtg atttatcagg
     5521 aaacactttt ttagatctgc tgcaaaaaga gtttagtttt actattacag aagaaggacg
     5581 attgaaattt gaaatatggc tttccattat gaagaagcga atgcgctgta cgaaaaaatc
     5641 caaaccagat tatagcaatc ttcctgttgg attgtttcaa acgacacaat ttttattgga
     5701 cctccataca tttttgaccc gttacttctt tcaatatgcc tttccttgga atcaaaatga
     5761 aacgttggtt ttttatcttt tcatgatcag taactttaca ttagatacac ataactcgca
     5821 tgtccaggag tttttgataa gcgaaaaata caaagacgac gtagtagctc aattgaatcg
     5881 aacgtttaaa caatcgatag aagaatattt ctctttttat gtttttccag aagagatttc
     5941 gcaaaagatt gcgttgacag tgatgcaaaa tcacgctcat tttgcctatt ttagaggatg
     6001 gatgaatatt tttggccaag aaggattaaa acagcggtta actgaagtag aagataaaca
     6061 attcctggtt ttatgtgaaa gattggtcag acaaacattt gctattgttg attttactga
     6121 agtagacgag gaaaatgccc gctttgaatt atttgctcgc tatgcatcta ttttgcggac
     6181 gctgatcaag cagttagaat tacgtctgca aattgcttat gatcttcctt atgaaaggct
     6241 aatggcggat atcgtgaaag aaatcatcca ggaacaattg ctgaatacga tcaaatatga
     6301 tttagtcgaa tacgagaaag aaaagcagta tgatatcatt ttgactagcc aattgaaaga
     6361 gtacccgtca cagaagtccg ctaaagtttt tgtattcact agcaacgaat ataattatga
     6421 tttcccctat ttgaaccaat ttttaaaaga atgttattta gaaaaattga atttaaggat
     6481 gaataaaaca tgaaaatcgt gatcattggt gcttcccatg caggcatcac agctgcccta
     6541 aatctaagaa aacttcaacc gaaagcagaa gttttactga tcgatgaaga tcataaagat
     6601 ggactaggct atgtttcaaa tggaatcaac ctttatttaa aaggaaagat acgtagttta
     6661 gctgaagttg cccataacat gcggactctt caagaaagtg gggcaaaact gatcaccgaa
     6721 tggagagtaa ctgaacttga tccagacaaa catcagctca tcctcgcatc caaagaagga
     6781 aaaaacgaga ccataaccta tgataaatta atcgtagcaa caggttcctc tcctgtcacc
     6841 ttgtataaac aaattgaagc tgaaaatgtc tacacctata aaaatctagt gcaatctaag
     6901 caagtacttg ccgctttaaa agaagcaaaa gaagtagtga tcttcggtgc tggttatatt
     6961 ggattggaat tagcagatgc tttgagaaat aaaggataca ttattcatct agttgattat
     7021 atgccgaatg ttttatcaag atatttcgat aaggatatga tcaacagttt tcaaaatcag
     7081 ctgcaaacta agcaaatcaa tttttatcca aatgaattct tgatcgattg gaaaaaatca
     7141 gaagagaaag tagtatccgt acaacttcta tctcaagcaa taaaggcaga tatggtcatt
     7201 ttttctgccc aaactcgtcc taatacgaca ttgttaaaag aaaaagtggc tttgtatgag
     7261 gatgagactg tgatagtcaa tgaatatctg cagacaagtg atccggacat ctatgcgatt
     7321 ggagatatcg tcccagtcag ttttgacaaa aataaacggc atctcttttt acctttagtt
     7381 actcgagctg tacatatggc aagagcagta gcgctaactt tgagtggtca gccaaccgct
     7441 tacgacctca gacaaaagat aacagcagca gtcattatag actacttttt aggaactgta
     7501 gggctgacag aagatgaagc gccattcttg gaacaaaata ccggttcgtg ttcaggagaa
     7561 tttgatctat ttccacaata cgatgaagaa aacaaaacag taaatgcaaa gctgatctac
     7621 catccggata cattagaaat aattggcgga cagctgataa gccaagaatt cttattatca
     7681 gatttgaacc tgcttgcgga tatcgtgaaa cacaaaacta cgattccaca gctagcagtt
     7741 gaggattttg gatttctgtc tgaacacacc tctcgttttc actacctcaa tgagttagct
     7801 tttaaagtac tagctaagag agcaaatagc aatagagtag ataaaagact gtgagaaata
     7861 tcacggtctt ttattgtgtg gggatataat aaatattaat caatcgagaa taaaaaattc
     7921 atataatgaa atagcggaag aattttttga gcataatatt cgccaaaaat ttatcaagtg
     7981 ataaaattgt tgtataaaat gattatttca ggaggaggag ttaaatgttt ttagcattaa
     8041 atgaaattat gcattcaaag ttacgttatg cgttagttgc aggggtgatg tttttgatcg
     8101 cctacctggt attttttttg actgggttag cttatggctt agctcaagat aatcgaacag
     8161 cagtcgacaa gtgggaggca gattcgattg tgctttcgaa agatgcaaat tcaaatctag
     8221 ggatgtccat gattactaag aagatagcag aagaagtcga aggtgggaaa gtggcgtatc
     8281 ttgcccagac acctggagtc gtgactagta aagacagtac agaagaagga aaaatcaatg
     8341 ttagcttctt cggtatcgat aaaaatcaat tcatcatgcc caatcttgtt gaaggaaaag
     8401 cttttgataa cgatgatgag gcagtaggcg atattagttt gaaagaagaa tatggtctag
     8461 ccgtaggaga tacagttaag ttatctggta gtgacaagac gtttaaacta actggcttta
     8521 cggatcatgc caaattcaat gtctcaccag ttctctatac aaccatcaat gcttaccaag
     8581 agatccgctt cgaaaaagaa gatacaagtg aaaatgcacg gattaacgca atagttgtca
     8641 gaggtaaaat cagtaattta cctgaagatt tagaacagat caaaatttct aaattcatca
     8701 atgaattacc tggatataat gcgcaagtat tgacttttgg atttatgatc ggatttttga
     8761 tcgttattgc ggctattgtg atcgggattt tcatttatgt actgacgatg caaaagatta
     8821 atatctttgg tgtaatgaaa gcacaaggga tcacaggagg atttattgcg cgttctgtag
     8881 tggcacaaac attcatttta tcatttgttg gtattcttct tggtctatta ggaacagtgg
     8941 gaacatccct tgttctacct gatgcggttc cattccaaag caattggctg ttttttggcg
     9001 tcattagctt gttgatgctg gtagtagctg tgttaggcgc actattttct gttcggacaa
     9061 tcgtaaaaat cgatccgttg aaggcaattg gataagaagg aggaagagca tgaaagcaat
     9121 agaatttaaa gaagtagaaa aaagtttcca agatggcgac caagtagtta aagcattgaa
     9181 agaaacaacc atgagtatcg ataaagggga atttgttgca atcatcggtc cttctggttc
     9241 aggtaaaagt acttttttga ctttggcagg cggattgcag acgcctagca gtggctctgt
     9301 atttatcaat gatcaagaat tcagtaatca aaaagaaagt gaacgcgtta aactacgttt
     9361 taaagaaatt ggctttattt tgcaagcttc caatctggtt ccttttctga cagtaaaaaa
     9421 gcagttatta ttagtagata agatcaaaca tgaacatcgt caaaaagaag caaaagaatt
     9481 atttgagcag cttggcgtgg ataagttgat caacaagtat ccagaagaac tatctggtgg
     9541 agaacggcaa agggtcgcaa tcgctcgtgc attgtacaat gatccgtcca ttattctagc
     9601 agatgaacca actgctagtt tggattcaga aaaggcacga gaagtagtcg atatcttggc
     9661 aaaagaatca aaagacaaaa ataaagcaac gatcatggtt actcacgata cacggttgat
     9721 tgacaaatgc gatagaatat ttatgatgga agatggtgta ttgaaagaaa aagaataacc
     9781 aaaaagcagg tggaaaaatg gtccatcgat acaacaaaga tacacagcaa aaaattttga
     9841 aagaagcgac tgacttgttt atgtccaagg gatatctcgg cacttcaaca agagagatcg
     9901 cacaaaaagt tgggatcacc caacccaatc tctatcatta tttcggtgat aaagaaaaac
     9961 tatatacggc agttttagaa aaccacttaa aagatgttgg aaaagctttg agagaaatcg
    10021 ttcaaacaag cgaaacggga tttcaagtaa ccttgacaaa aatggcgcag tttttgattg
    10081 agacacatct ggtcgatttg tttctgatgc ttcacgatct tgaaagcaat ctttcaaagg
    10141 aaacaagaga tcatcttttc tttctatgga agaaaaatta tcgtgaacct tttgaagaaa
    10201 tcttttcagc aaaccaatct gtgctaagaa atgggatttc aaaagaaatc gcagcaaggc
    10261 attttttctt agtgttagca ccttacatca ctcggtcgaa aagacctgtg gaagaaccgc
    10321 taaatgtggc gcaattgatc gatttatatt tgaatggtgt attaggagaa gcgtaaagct
    10381 gttatatagg gagaaaaata gttaggtgaa gaagcaagat atgacgaaga tcgttatatc
    10441 ttgttttttt tatataggta gtttttcata aatacaaatt tgttattctc tcatgattat
    10501 cagggtatat ttttatttat tatgtcggtg ttccaggaag tgttttaatt taataacgaa
    10561 gaaattaatt gtttgtagaa tgaaggaaac tatttattgg gtagatgaat caccacgaat
    10621 agtcaaaagg agatttcagg atatgccaaa aatgaaaaaa atcattttag cagtaatgtt
    10681 attttctacg gttatatctt ttacagcccc ttttttgcgt acttaagctg attttaagtt
    10741 ttgaccctta gtctggatct acgaggtgag agtcaagaat gaaaaaaata attggagctt
    10801 ttgtatttgc aatcattatc attattatct gttttcaaat agatgttttt ggtcaaaaaa
    10861 agatagttgg acaaacggtc agcagtacga acagtacggt tacaaagaca aaggaatcat
    10921 ttgatgatac agaacaagag acattaatca aaatcggaaa aaatgaccgt acactatata
    10981 cagatgactc gctaactacc ccacttgcaa ctataaacgg cggagagctg gcggagtttc
    11041 tttctgaaac aaaaaatgct tatcagatca agacaaatga tggctatact ggatatttag
    11101 ccctcgaaga tggaaccaaa gtcacaaaga atatacaaac aaaaccggaa gagctatctg
    11161 atgcagtgat tgtattagat cctgggcatg gcggaaatga tactggggca ttaagcaatg
    11221 atgaacaaac agaagaaaaa gatataacat taagtacagc cataaaagta aaaaaggcgc
    11281 tagaggatgc tggagcaacg gtttatttga cccatactac agatgaatta gtacagcttg
    11341 gagatatatg cgactacagt gaagaaaaaa aggcagatgt ctttatcagt ttacacgcag
    11401 actctacgga atatgcgaat gaagcaactg gaatcacgac atattattac tatggacaag
    11461 aggaaacatt agcacagaca attgctgact ccttcactga tctgcctctg gattcaagag
    11521 gaatctccac tgggaactat caagttcttc gagaaaatct tcaaccttct attttaatcg
    11581 agatgggtta tatgaataat gatagtgatt taaaggaatt agtaacagac agttatcagc
    11641 agcagattgc tgcaagtttg acacaagcat tgaccactta ttttcagtaa taaataagcc
    11701 cagccaaaca aaaatagctc ctcatatttt tcgtttggcc gggcttatta tcgcagatgc
    11761 aagcttttga acaccgttta aggaagaggt tgtgagcctg ccgtttaact ccgcgtcaca
    11821 aggagcaatt tcaaaaaaca gcttctcatg tttgatgact attgtgactt gtgaccgagg
    11881 agttggcatg agaacaccgt ttattcggaa caagggactg tgacatgact tttgtcacag
    11941 ttccattatt tttttagcag cttcaaagct ttttctgtat tggcaaaatg aagtttggca
    12001 tatttttcaa gcataggcat gccgcctttc tccaatagtt ttgccgctac ttggtcagat
    12061 ttcgttccag ctcctgatgg aagggtgatt ccagcttcag ctgatgcttt gtgtaactct
    12121 tccaaaaagc tggcacgact gatgatagaa gcagcagcaa cagctaaatg atattgttct
    12181 cctttagtaa cgaagtacag tttttcttgt aattgatttt tttctaaacg cgcatatttt
    12241 ttaaaatttg cttccggcgt aaactgatcg atcaaaatcg cttctggttt ttctggctcg
    12301 atttttttta gcagcaaatg gatcgcttga ttgtgcaaag cgactttcat ccgaacggca
    12361 ttgtattttg gttggatctc attgtatttt tctggagata agacaagcag tttatagggg
    12421 atggtttttt ttaagatgtc agcaagctgg atgatttggc tatcttttaa ctcttttgaa
    12481 tctctaacac caagtttacg taattctgca agctgatcac gtttcacata agcagcacat
    12541 actgttagcg gaccaaaata gctgccattt ccgacctcat cactacctaa aacggataat
    12601 tgactgatat tttttggcaa agaagaggct gtttttgctg gcttggcttg tttatcctgt
    12661 gcctcccccc atttatcagc ttctattgct gcattcgtac cttgaaacat cacttttccg
    12721 gatgtataag cggtaatggt cgtcccgtct ttttttgcag caaagctagt ataaggaatc
    12781 tgtttattta acaaataaga gcgatagtat gctttcattt gactaagttg gtcgtttgtt
    12841 gctttgataa tgacgttgct cataaaattc tcctcgtttt tatacataaa actataccgc
    12901 aactcacgac ttttcgtttt ctaagagatt gcgagacata gcattttttt aaagtttatc
    12961 atataatagt taagtatgtg aaaaatattt gtggagaacg gtgaaagaac caacttcatg
    13021 gtacactaaa taaaactttc atcaaatagg aggaaatgat tcgtatggcg cacgaaaaaa
    13081 cacgctacaa agcagtgatt gccaaccaaa catatactat tatcggacgg gaaaccaagc
    13141 atcatatgga tatcgtaacg aagctgatca atgaacagtt agcagaattg aaacaattat
    13201 caccacaaat ggataatgaa caagcagcaa tcttgatggc tgtcaatgcg ctttcggatc
    13261 aattgaagaa acaagaaaga atcttggagc tggaagaaga aacagcagaa ttaaagaaaa
    13321 aaatgatcaa atttacagag cttgaaaatc gtgtgaaacg cattgaagca atcgagaacg
    13381 aagctcggga agtattgaaa gaaaatggtc aagcagatca taccattaaa aatcatgtgg
    13441 aagcacaaca gattttaaac gagaaacgaa aaggacaaat taaacaaaaa tcttctaatt
    13501 aagatccttt tatcaaaata agaaaaattt aaaaaagtta gaagagaaag gaacgtagca
    13561 tgctgagttt gctgatttta tttttattgc taattgcgtt tttttctgga gcacgtcgcg
    13621 ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt gtcatttatt gctgcccagc
    13681 atttttacaa accattagca agtcatttgg aattatacat tccataccca gctgtcaccc
    13741 caacatcaaa gttagcattt tttgatcagg tatttgcttt tcacctagat gaagcttttt
    13801 atgcaggaac tgcttttctg attattttgc taattggctg gctgcttacc cgatttgtgg
    13861 gggtttttgt ccatggattg acgtatgtgc cgatcttgag acaggtagac tggattgcag
    13921 gaggcatcct gtcactaatc atggcttacg ttactatatt tttgattttg cagttacttg
    13981 cctttgttcc attagatgtg gtccaaaatc aattcggcga gcatagtttc gctcgtttta
    14041 tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta acgaatattt
    14101 tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta gtttatttaa
    14161 gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga taaggtgata
    14221 aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag atggtcagac
    14281 aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca gtgagtgaga
    14341 aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc
    14401 gaggcgggat ccctatacct aaacttgaaa atatccgtcc ccatatgaaa cggatcgaga
    14461 tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg tctacgacaa
    14521 gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga aatcgaattt gcacgtttgt
    14581 atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa
    14641 tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc ataagacaga
    14701 acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa
    14761 atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac gttatcccag
    14821 tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt gcttcaggac
    14881 agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc
    14941 aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct gaacttgttc
    15001 cccatcgtca agaaatcatc cacaatgctt atgtgattgg taaaatggat ctgatgaatg
    15061 caaaagcacg atttggtaaa gaagtaaaag ccatcgtgcc gggaatcagt gaagacaatc
    15121 atgtcgtctt gaaacaagca agacatccac tgatcgatca ggaaaaagta gtttctaatg
    15181 acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac acaggtggga
    15241 aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca ggattaccga
    15301 ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg gatataggag
    15361 acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg aacactgtag
    15421 atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa
    15481 cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt
    15541 ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga tacaatcggg
    15601 caaatacgat taatgccagc atggagttcg atgtggatac actaagtcca acgtatcgtt
    15661 tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg
    15721 atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa gatttaaacg
    15781 aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat ctggaaatgc
    15841 gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct tacagctatt
    15901 tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaac gaagtcgtgt
    15961 cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga
    16021 ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc
    16081 tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa
    16141 cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg acgcttttac
    16201 gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag
    16261 acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc catgctgtac
    16321 gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa cgatacgaag
    16381 aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt tatcctcaag
    16441 tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact gattatctta
    16501 aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg
    16561 cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc gctatccaag
    16621 tatagaaatg ctatactaat catagatttt aaaaatggag gtctttgata tgtcacaagt
    16681 aattacagat aagacattta atgaagaaac agataaagga cttgtattga ttgatttttg
    16741 ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt tggaacaaga
    16801 atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc cagaaactcc
    16861 tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg gtcaagtagt
    16921 agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg atcaatattt
    16981 ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc gtcctcggtt
    17041 tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat tacgttgtca
    17101 ggctggattg tttttttgac agacatgcta taataacttt aattgaatat gaaggggagt
    17161 accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag cccggctgtt
    17221 acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa atattaaaaa
    17281 atcgtgtccg ctcatatttt actggcagcc ataatacgaa aacggaacgg cttgttagtg
    17341 aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg ctcttagaga
    17401 tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat gataaaacct
    17461 atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact cgtaaagtac
    17521 taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg aatgagacaa
    17581 agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag acgaaggagc
    17641 cttgtcttta ctatcatttg ggacaatgtc tgtgtcctta ttatttcgac gtagatccga
    17701 aagtctatac gaaaatcgtt gatgaagtga aaaagttttt aaacggagga cacgaaaaaa
    17761 tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag tttgaaaaag
    17821 ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc caaaaaatga
    17881 caaatactga tttactggat cgagatgtat ttggttatgc gattgacaaa ggatggatgt
    17941 gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt tcaatgtttc
    18001 ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt tatcaggaaa
    18061 atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag cccagtgtag
    18121 aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa aaattagttg
    18181 aattggcgaa taagaatgcc tcagttgctt tgaatgaacg gtttgatctg attgccagaa
    18241 aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc
    18301 caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct gtggctgcga
    18361 tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa
    18421 cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc agatattcac
    18481 gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga ggaaaaggac
    18541 aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt cctattgctg
    18601 gcatggcaaa aaacgataaa cataaaacaa atgagttgct attcggtata gatttggctg
    18661 tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc caagatgaag
    18721 tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt tttgcttccc
    18781 gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca
    18841 aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc ggactgccga
    18901 aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag ggcactttac
    18961 aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta cgttttgttg
    19021 gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct tgaagaagga
    19081 gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac acagctgtcg
    19141 atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc caaaacgggg
    19201 ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac caaggagaag
    19261 tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt tatcttccgg
    19321 aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat tttgcagaac
    19381 ttagagggaa atcaagaaaa gagattgaac caaaaatcga tttttggatg gaaaaatttc
    19441 aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat cagcaaaaag
    19501 tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat gagccattca
    19561 gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa ttgaaaaaac
    19621 atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa atctgcgatc
    19681 atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg
    19741 aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag gaagtagaag
    19801 aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag attaccttag
    19861 aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc tacattccta
    19921 tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca ggtgaaatcg
    19981 atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa gtcgatttct
    20041 ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt agcaggaaac
    20101 tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa tgagcaaatc
    20161 agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt tgattctgaa
    20221 gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt agtctcaact
    20281 gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca aagtacacaa
    20341 ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcaag tcaattaggc
    20401 atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg acaaaaagtc
    20461 aattttgatt caaaaggaaa aatgacagtt ggagaagata actcgacagt ccaatatgtc
    20521 gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc gcagatcatt
    20581 gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct gtccagtacg
    20641 actgcacaaa agcattttta cggaaaacta acaggtgttt tacttgttgc attaacgcaa
    20701 atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga tatggaaatg
    20761 gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt cttgtggtat
    20821 tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc tttatgtggc
    20881 tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac ttatctatct
    20941 ctaggcggat atatgcttgg attgatacta ggagcttcag atccaaacaa tatcgttatt
    21001 cggatcactt cgtatattcc attcttatct tcatatatta tgccggttcg cttagcaaat
    21061 gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc cgtaacattc
    21121 ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgtgta tagcgaaggc
    21181 ggattatgga gttcattgaa acaatcgatt tcaattatga gaaatgaacg aaaaaaagca
    21241 taaaatatag aaaaatatag aaggaagctg gacatttatt gttcccagct tcttttttgt
    21301 atagacctat ttctattttt ttattatatt ggatatatat attcattgaa aatagagcat
    21361 aacaacttta taatgttgag tataccaatt tttggaggcg ttttaatgga ccgctattta
    21421 gatttacttt cagagaagtt ccctacaacg gaagatgtca tcacggaaat catcaatctc
    21481 gaagcaattt tgcagcttcc aaaaggaaca gagttatttt tgagcgatat acatggcgaa
    21541 tttcctgctt ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga aaagatcaaa
    21601 gaccttttct ctgaacaaat gacggaaacg gagatcagcc aattcactat ttttacggct
    21661 tatccagaat atgtgttgac gactgattgg tacaaaaaac aagataaatc ccagctgatc
    21721 catcgtttga tcgatctttt acggtttacg acagtcaaat acactcgatc aaaagttcga
    21781 aaaggattgc cgaaagaata tagctatatc attgaggaac tcttgtatat cgatgatcgt
    21841 atcaacggga aaaaagacta tgtaaaaaaa atcattgaac aacttgtctt gatgaaggaa
    21901 gaagaaaaat ttttaacaaa acttgcgcag acgatccaaa aatctgtcat cgatcatttg
    21961 catatcgtgg gtgatatttt tgatcgagga actcaagcag ccaaagtaat ggatcgaatc
    22021 atggattttt cctctgtaga tatccagtgg gggaatcatg atattttgtg gattggtgcg
    22081 tattgcggct ctgaggcatg tttattgacg cttttacgga ttgccgcacg ctacaactat
    22141 ttatttgaat tagagaaaga atatggatta aatttacgtc cattattttc ttttgcccat
    22201 caaacctatc agaagaatcc agtatttacc cctaaaaaca ggaaagatct atctgaaaga
    22261 gagcaagaag aattagaaaa gatccatcaa gcactttcta tcctccaatt caaatcagaa
    22321 catcagttgt tgaatcgacg acctgaattt aaaatggacg atcgtaaatt gttggaaaaa
    22381 atcaactatg aggaatcaac gatcgtttta gaaggtcagc tctatgagtt gaaagggact
    22441 tgttttcaaa cgatccagcc agatgatcct aaaaaattct tggaagagga acaaaaagtg
    22501 atcgatagtt tgatgtattc attccagcac tcgttaagaa tgcaaaaaca catgacgttt
    22561 ttgatcgaaa aagggggaat gtatctcacg aacaatcagc atgtactgtt ccatggctgt
    22621 atacctgtag atgaaaaggg gcagctctta gcatttgaat tagaccaatc ttatcgcgga
    22681 aaagaactgc tgggattttt tgaaaaacag ataactgaaa gcgcaaaaga tttgactgca
    22741 aatgaagatt tggcgaccga tttgatttgg tatgcgtgga gcgggccatt ttcgccttta
    22801 ttcggcaaga gtaaaatggc aacttttgag cgctattttc tcacagagcc tcatacgcac
    22861 gtcgaaaaaa gcaatcccta ttatcacttg cgagatgaag agtggttcag tgaaaaaatt
    22921 ttggcagaat tcgatgcaaa agaagaaggt tcagcaatca tcaatggaca cacgccagta
    22981 aaagtaaaaa aaggagagtc accgatcaaa gcaaacggaa aagcatttgt tattgacgga
    23041 ggtctatcca aagcttatca aaaaacaacc gggattgctg gctactcatt actatgcaat
    23101 tcttacggtt tccaaatcgt gacacattat ccgttcttgc ctattgaaca acaatttgca
    23161 aaaaaatctg atcaaacgaa tgtaaaaaaa gtgatcgagc agcagctccc acgcaaactt
    23221 aatcggaata cgacaaaagg aatggaatta cagcaacaga ttactcaact ccaaaggctg
    23281 ttggattatc gaaaggacga ttgagaaaaa tgctagccaa cattgctcaa tagactaaaa
    23341 agcgactgca acaaaaggcg tgtcgcagtc tccttcggaa ttagctcaaa aaacggtaaa
    23401 atattagaag ctatttttct gtttatttct ctaatactca ggtcagggcg ctgatttcac
    23461 gacctcttcc ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccaa
    23521 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct
    23581 cacgacctaa tcagaaaaac gattaaaatt cattcacaaa gaaatcgaat aaacgctgtt
    23641 cagatgaatc tacacgatga gaaagttccg gatgccattg gacaccaagg attttgtgca
    23701 tattatcttt actttggact gcttcaatga gtccatcgtt ggaaaaggcg atggctttta
    23761 acaaaggaga aagatctttg actgcctgat gatgatagga attcacaaag tatgaatcag
    23821 ggagaaggtc acttaataaa ctgtccgaaa cgattttgac ttcgtgagtt gcaaactgcg
    23881 gggcagtggg ctgttgttcg tgttttacgg accattttgg atataatgaa agatcttgat
    23941 aaagtgttcc ctccaacacg acattcaata gttgaagtcc tcggcagact gcaaaaatcg
    24001 gttttctttg tttcaatgct tccaagatca atgcctgttc gaattggtct cgttgaatat
    24061 ttgtttccgt cagttttgga tgagggtctt ccatgtaaag ttgcggacta acatcctgac
    24121 cgcctgcaag aatgagtttg tcgattttct caattagttg aggcgctgaa tctggagaca
    24181 tgactggcag aataagaggt aatccttgag catctaaaac tgcatccaca aatccctgcg
    24241 gtgtatagct tacttgattt ccttggaaag tatctgtcgc tcgaatcagt tgattcccgg
    24301 caatgccgat gattggtttc atttttattc cccctaaagt agactaattg taacaaggat
    24361 ttaaaaaaat ttaaactatg taagacttgc ccctatactt atctgttctt gctgtgttac
    24421 tatctgtaaa gagaattttc gtgtgaaatt catgacgata gttgaaaata ttgcttaatt
    24481 tgttataata aaaagtaagc agaaataaaa aggttgggcg taagcttcaa ggatctagtt
    24541 cttcaagggg tgagaaggac attttattga aggggcctgc ctatactaag ttatctttag
    24601 aggtacatac tttcaatgaa aaaagaaagc atgtacctct tttttaaatc gaaaggaagt
    24661 gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa atgagaagcg
    24721 tgctcgaacc ctacggcagc agttgaagac ctatgcagaa gaaaaagagc aagaattgga
    24781 gataatccaa acgaacagtc ggcaacagct gaaacagttg gtagaagaaa aagaacaatt
    24841 cgaaaagcaa gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag acaaggtgca
    24901 agcaactttg aaagaccaat atcaaaagca tcagagtcaa gcaatcggtg caatcattga
    24961 aagggtgaaa gaaacttatg gccgtcacta agatggaaaa agtgaccctc atttcagata
    25021 aaagaaatca ggagaggatt ttacaagcag tacaaggaat ccaaaatgtt gaaatccggg
    25081 atctgtatca aaatagaaca aacaaccagt gggtagaaaa atattttcca gataccacga
    25141 ttattgataa agaagcaaaa ttaagcgctt taggcaatcg gctgaaagac atccaagaag
    25201 ctatccaatt catacaacac cacggcgata gccgtcaaaa gaaactccat ttgaaacgtc
    25261 aagaactttc cttacaagac ttggaagcaa attattccga agaaaatttc atgaataaat
    25321 tagaagaagt ccttgattta aaagaaaagt gggaacagct aactgaaaaa cgtgatcgtt
    25381 tagaagaaga ggaagaatgg cttcttcatt ggcaacagct ggatattgct ccaagtatgt
    25441 atgcaagtaa gacgaccatt tttgagatgg ggactgtgag tgcagcaaat tttgaaagtt
    25501 tcaaggaaga actaagtaag cttgatgacg tttatatgga agaaatagat tatacgccaa
    25561 aacaagttta tctttcgtat gttgctttaa aaaaatcagt tgctgctata gaagaaattg
    25621 ctgggaaata cgggttcaat aaagaaacgt atccttatga acaaaatcct aaagaaaaat
    25681 tgcaagaaac caaacagcaa ttatccattg ttcacgagca gcaaaaaaag ataacgacag
    25741 cgttaggtca gtgcagcggc tatatccaag aatttgaatg ggtagaagaa gtcacacttg
    25801 ccgttgcaga acgggaaaaa gtaaaagaac ggtttatcca tgccacttat ctgatcgttt
    25861 tgcaaggttg ggtagatgca gaagaaaaac agaatttgat gcatgtgtta aacgaaactg
    25921 tatcagaaga agatatatac gttagtttcg aaacaccaaa ctcagaagag attcagcagg
    25981 aagtccctac taaattgaaa aaccatccgc ttatcgaacc ttttgaattg ctgactgaaa
    26041 tgtacagtct gccaaaatat gaagaagtag atccaacgcc ttggatgaca ccattttatc
    26101 ttgtcttctt cgggatgatg gtcgcagatg ttggttacgg attgctgatg ctcattggga
    26161 caatactagc tcaaaaacta tttgttttac cccgcgggat gaaacgtttc gtaaaattct
    26221 ttgagattct ctcggtacca tcgatcatct ggggattgat ctacagttca tttttcggac
    26281 aggcattacc aaaagaacta tttggtatcc gcttgccttt cccaatccta tctacgacgg
    26341 atgacgtcaa tacgattttg attttatccg tgattttcgg tctgatacag atcttggttg
    26401 gattattcgt ggcagcaaag gaaaatatca ggaggaaaga ttacttgagt gctgtcagtg
    26461 atggttttgc ctggcaaggg atcctgattg ggatcgttat tgcattgatt ggtgtcatgt
    26521 tgataaaaaa tacagcattt gtttatcttg gcgcggggct atcgatcatt gctgctttat
    26581 gtatcttaat cgtgccgatt atccaatcgc cgtctaaagt caagggtgct gccaaaggag
    26641 cgtataatct ttacggattg actggttata tcggtgattt agtcagttat acacgtttga
    26701 tggcactagg tatttcaggc ggaagtattg gcgcggcatt caatatgcta gttgctttca
    26761 tgccgccagc cgctcgattc agtgtaggga tccttttgat catcgcttta cacgcactga
    26821 atatgttttt gacactgtta agtgcctatg tccatggtgc acgattgcag tatgtcgaat
    26881 tttttggaaa attttataca ggaggcggac gtgcctttga cccgctgaaa acggcggaaa
    26941 aatacgtcaa catcaatcat aagaaaaaaa acaataaata attggaggat ttttatacaa
    27001 tgatggatta cttaattagt caaaatggtg gaatggtatt tgcagtatta gcgatggcaa
    27061 cagcgacgat tttttcaggg attggttcag ctaaaggtgt tggaatgaca ggtgaggcag
    27121 cagcagcgtt aacaacaagt caacccgaaa aattcggaca ggctttgatt ctgcaattgc
    27181 ttccaggtac gcaagggttg tatggattcg ttatcgcctt tttgattttt atcaacttag
    27241 gaaatgatat gtcagttgta caaggattga actttctagg cgcttctctg ccgattgctt
    27301 ttacaggttt gttttcaggt atcgcccaag ggaaagttgc agcagcaggt atccaaattt
    27361 tagcgaaaaa acctgaacat gctacaaaag gaatcatttt tgctgcgatg gttgaaacat
    27421 atgcgatttt aggtttcgtt atttccttcc tattagtatt gaatgcctaa gcataaagga
    27481 ggaaaagcag atgaacgcca ttgaaaaaat catctctcag atgaatgaag cagcagagca
    27541 agaaagagcg gcgttagagc aagaagagcg aatgaagatc gatcaaaact ttgaacaaaa
    27601 acgtacgcaa atcgaaacag aacatcaaaa acaaaaagaa aaacaaatcg aattacttga
    27661 aaaaaaatac cgtcagctta ggaatcgcca acaagtagaa gtacgacaag aaaatttgaa
    27721 tgcgaagcag gaatttttac gacgattgtt tgcagatgca atagctgaaa tggagaattg
    27781 ggatgaatct gaacaaatcc agtttatcaa aaattcattg tattcattac ctttgacagg
    27841 caaagtaaca ttcattgctg gagaaaaatc agcagcttat ctttctcaga cattgctgga
    27901 tgaatggaac aatgaacttc cctttatgat ggttttaagt gatgaaactg ttgctgatca
    27961 agcgggtttt ctgataaacg atcaaggagt tcagtataat tttcttttca gcagccttgt
    28021 acaagatatc caaggaacga tgagttttga gattgccaat caattattcg aataaggagg
    28081 gatcagtgta tggaatacca cgaactaaat cccttgattc gcggaagaga gcttgaactt
    28141 ttgtccaatg agacatttga acggatgatt caaacaaatt ctgtcgaagc acttggtgaa
    28201 atattgaaat ccacgattta cagtccatat atttatgacg gatatgaata tgacttcgaa
    28261 cagaatcttt taaaggagca gagccgtcta tttcgctggt taaaggaatc agcaccggag
    28321 cctgaaatcg tttggatcta tacgatgcgc tatactttcc ataatctgaa ggttttgaca
    28381 aaagccgaga tgacaaatca aaatctggac catttgtata tcgacgatgg attttattca
    28441 ttagaaacct tgaaggatgc cattcatacg caagtttcaa atgaactccc tgaaagcatt
    28501 atggacagta tacgagaagt acgtgattat tttgaagagt ctaccattct tcagggaatt
    28561 gacgtgattt atgatcgaca gtttttgaca gagcagcgac ggcttgccga taagctagga
    28621 tatcaagaat tgctgacaga agtcattgct tttattgact tgaccaatat cacaacgatg
    28681 gctcgaggaa tattgcagaa aagaacatat gggttcatga cgacggtact ttccagctcg
    28741 ggaagtatcg aaaaagaagt atttttgaac tttgtagata aagatatgga agattacacg
    28801 aattttctat tagagacaga ttatgctgat tttctgcgcc ctgtcatccg tgagggaaat
    28861 cttgatctcg taaagcttga acagttgaaa gatgattatc taagttcatt gtatcaagaa
    28921 gcacaaaccc aggctttcgg accactgcca ctactagcct ttttgaacgc aaaagaggta
    28981 gaaagcaaaa accttcgttt actggttatc ggcaaaagaa gccatttcga accagaaaca
    29041 atcagaaagc gggtgagaca agtctatgac gcataagatc ggtgtagttg gcgacaaaga
    29101 ttctgttttg ccattcaaat tgtttggctt tgatgttcgt tatggtacaa ccaagcaaac
    29161 cgttcgtcag gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt atgtcacaga
    29221 gcaatgtgca gcattgagcc cagaaacgat cgaacgttac aaggaacaaa tgatacctgc
    29281 tatcgtattg attcccaatc atcaaggatc attagggata gggctggaag aaattcaaaa
    29341 aaacgtagaa aaagcagtag gtcaaaatat cttatagtat gaaaaggaga tagaaatttt
    29401 gcaaattgga aaaatcataa aagtctccgg acctcttgta atggctgaga acatgtctga
    29461 tgccagtatc caagacatgt gtctagtggg agatctagga gttatcggag aaattatcga
    29521 gatgcgtggc gatgtggcgt ctattcaagt atatgaagag acttctggaa ttggtccagg
    29581 cgaaccggtt cgctctactg gtgaagcttt atctgttgaa ttaggaccag ggatcatttc
    29641 acaaatgttt gacggaatcc aacggccatt agataccttt atggaaatca cacaaagtaa
    29701 tttcttaggg cgcggagttc aattgcccgc attggaccat gagaaaaaat ggtggttcga
    29761 accaacagta gaagccggag aaacggtgag tgctggagat gttatcggga tcgtagaaga
    29821 aacaaaagtt ataaaacata aaatcatggt gccaaatggg ataaaaggaa cgatcaaaac
    29881 aatcgaaaaa ggttcattca ctattgatga aacagtatgt gtcattgaaa ccgaaaatgg
    29941 tgacaaaaat ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc caatcaaaga
    30001 aaaattaaac ccggatgcac ccatgatcac tggacagcga gtgattgata cattcttccc
    30061 agtgacaaaa ggaggagcag cagctgtacc tggtcctttt ggtgcaggga aaacagtcgt
    30121 tcagcatcaa attgcaaaat gggccgatgt ggacatggtt gtctatgtgg gatgtggcga
    30181 gcgtggaaat gagatgacgg atgttttaaa tgaattccct gaattgattg accctaatac
    30241 aggagaatcg ttgatggaac gtacggtatt gattgctaat acatcaaaca tgccagtagc
    30301 agctcgagaa gcttctatct atacgggaat cacaattgcg gaatatttcc gtgacatggg
    30361 ttatgacgtt gctatcatgg cagactccac ttcacgatgg gcagaagcac ttcgtgagat
    30421 gagcggacgt ttggaagaaa tgcctggtga tgaaggttat ccagcttatc ttggttcacg
    30481 gcttgcggaa tactatgaac gatctggaag agtcattgca ttaggatcag aaggtcgcga
    30541 aggaagtata accgcaatca gtgctgtatc cccgtctgga ggggatattt cggaaccagt
    30601 cacacaaaat acattgcggg tcgtaaaagt attctggggt cttgattcca gtttagccca
    30661 aaaaagacat tttccttcga tcgattggat acaaagctat tctctttatt caacagaagt
    30721 agggaagtat atggatcata tccttcaagc tgactgggca tccatggtca ccgagggcat
    30781 gcgtattttg caggaagaac agcagctgaa tgaaatcgtg cgtttggtag ggattgattc
    30841 cctttcagat aatgaccgtc tgacattaga agtggcaaaa tccattcgtg aagattattt
    30901 gcagcaaaat gcttttgatg atgtcgatac attcacttca agagagaaac aattcaaaat
    30961 gttgaagatg atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt taggtgctta
    31021 tttaagtgag atcatggacg gtaccgtatc gattcgtgaa aggatcggac gaagcaaata
    31081 tatcccggaa acagaattag aaaaactaga tcaaattatt ggtgaaatca agcaaacgat
    31141 gcaaacgatc gtttcagaag gaggcatgag caatgattaa agaatatcgt acgatcggtg
    31201 aagtcgtcgg tccattgatg gcggttgaaa aagttgctgg tgtcaaatat gaagaattaa
    31261 tcgaagtccg tatgcaaaat ggcgagattc gtcgtggaca agtattggaa gtccaagaag
    31321 ataaagcaat ggtccaaata ttcgaaggaa caagtggtat caatttacgt gactcttctg
    31381 tacgattttt aggccatcct ttagaactag gtgtatcgga agacatgatc ggtcgagtat
    31441 tcgatggttt gggacgaccg aaagataacg gaccagagat cctgcctgaa aagcatttgg
    31501 atattaacgg agaagtaatc aatccagtag ctcgggatta tccagatgag tttatccaaa
    31561 caggtatctc tgcaatcgat catttgaata cattagttcg tgggcaaaaa ttaccagtat
    31621 tttctggctc tggtcttcct cataaagaat tagctgctca gatcgcgcga caagcaacgg
    31681 tactcgattc atcagatgat ttcgccgtag tctttgctgc tattggaatc acttttgaag
    31741 aagctgaatt tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt tctgtcatgt
    31801 ttatgaattt agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt atggctttga
    31861 ctgctgcaga atatcttgct tatgaaaagg gaatgcatgt tttggttatc atgacagaca
    31921 tgaccaacta tgcagaggca ttgcgagaga tttctgcagc acggcgagaa gttccaggaa
    31981 gaagaggata tcctgggtat ctttacacga acttagctac tttattcgaa cgagcaggaa
    32041 gaatccgcgg actaaaagga tcggttactc aaattccgat tctaacaatg ccagaagacg
    32101 ataaaactca tccaatccct gacttgactg gttatattac agaaggacag atcatcttga
    32161 ctagagagct ttacaagagt gggattcagc caccaattga tgtactcccg tctctttcgc
    32221 ggttaaaaga taagggaact ggacccggaa agacaagaga agaccatgca gctacgatga
    32281 atcagctttt tgcagcctat gcacaaggaa aacaggcaaa agaattagcc gtagttttag
    32341 gtgaatctgc tttatctgat gtagacaaga tctatgcgaa gtttgctgag cggtttgaaa
    32401 aagaatatgt caatcaagga ttctacacga accgttcgat tactgaaaca cttgatctag
    32461 ggtgggaact gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa gacgacatgc
    32521 tggacaaata tctgccagaa gggaagtgag ttctgatggc acgattgaat gtgaatccga
    32581 cccgcatgga attgactcga ttgaaaaaac agttgacaac tgccacaaga gggcataaat
    32641 tattaaaaga taagcaagat gaactgatgc gtcaattcat cctgttgatt cgaaagaaca
    32701 accagctgcg tcaagaaatg gaagagtcta tgacagaagc aatgtctgat tttgtattgg
    32761 caaatgcttc gatcacggaa cctttcatcg aagaattatt tgttttgccg gcggaaaacg
    32821 ttgatttgtc tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg atgaattttg
    32881 aatatgatga acagctgaca gacacacctt tggaatacgg gtatttgaat tccagtgccg
    32941 aactggatcg gtcaatcgat cggtttacta ccctgcttcc aaaacttttg gaactgacag
    33001 aaatcgaaaa aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt cggcgagtca
    33061 atgcattaga atatatgacg atccctcaat tagaagaaac gatctattat attcgtatga
    33121 agctggaaga aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa aatatgggta
    33181 ccactgacta agtggtatct ttttttaaaa gaggaaaacg atgattaaac aaagcaaaca
    33241 aataaagaaa cgtttgccgg ctgttcagct tctcgcatta ggattttttc tcctgatttt
    33301 agttggcggc ggcctactta ccctaccatt ttttagtaat agtgggcatg ggacaaattt
    33361 tatcgatgcg cttttcactg caacttctgc tgtctgtgta acgggattga caacattgaa
    33421 tacagcagag cattggaaca gtgctggtca atttttgatc atgcttttga ttgagatcgg
    33481 cggtcttggt tttatgatgg ttccgattct atgctttgca ttgataaaga agaaagtcag
    33541 tctcagcaca cgtattatat taaaagaagc gctgaatcta gaagaagtat caggtgtgat
    33601 gaagctcatg ctttacatcc taaaattggc ctttgttatc caattgattg gtacggttgc
    33661 tttatctttt gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt acagtttgtt
    33721 ccatgcaatc tctagttttt gtaatgcagg gtttgatttg ttaggagaca gtttagccaa
    33781 tcaccagcaa aatgtttatt tgatcatggt ggtatctgct ttgatcattt ctggtgggtt
    33841 aggcttcatc gtatggcgtg atttactgga atatcatcag aaaaagaaga tgacgattca
    33901 ttcaaaaatc gcattaaccg ttacaggtac ccttttatta ggcggattcg ttgtgttctt
    33961 tttcacagag cgcaatgggg caacgctagt ggaagggaat atgtttcaac gattggcaaa
    34021 cacctttttt atgagtgtga cacctagaac ggctggatat tattcagttg attacctcca
    34081 tatgagccat gcaggtctga ttttaacgat gtttttgatg tacatcggcg gaacatctgg
    34141 gtcgacggcc ggcggattga aaacaactac gttaggtgtg ttgctgatcc agatgcgttc
    34201 gatgttgaaa ggacgtacga gagcggaagc atttggccgt accatccgac aaggcgcagt
    34261 gttgcgggca cttactttat tttttgttac gttgacgttg tgtgtagtcg ccatcatgat
    34321 tttatcggtg actgaaacga tacctaaaac ttctggcatc gaatatattg cttttgaggt
    34381 cttctctgcg tttggtacag taggtcttac gatgggattg acgccagact tgactgcaat
    34441 agggaaattg atcattatag cgcttatgta cattggacga gttggtgtat tgactgtggt
    34501 cttctctctg atgacaaaag cacaaagaac acaatcaaaa tacaaatatc cagaagaaag
    34561 tgtcatgatt ggataatttc atcttctgac agaaacctag tgatttagta aaataagaaa
    34621 ataaaataat ttttcttgct tcttggatac tttaagtgca aaatgttcgt ttattttttg
    34681 aggcactacc cgttgttgaa cctcgggtag tgcctctttt ttattgaaag gaatcaataa
    34741 gatttgattc ctttatttat caataggtta taattcatat ttttttattt tttcttttac
    34801 gatacttagt ccagaagcta aaatttcaag gttattagag gatagggaaa ttcgaacgaa
    34861 tgcatctggt tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga taccgttagc
    34921 aagaaagtca ttctcaatta tcttagggtc aatctcagga tcaactggaa gaattcgata
    34981 gtaagggaca cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa gctgattggc
    35041 tctttttgta agacttaatt tagtttctaa gatcctattt gcttcatttg aatggaggat
    35101 ttgagtgatg acttccacat ctaatccaga agttttcaca tttatattaa aaattgcttg
    35161 ttctatcttg ctttggacat gtactggaac tacaagataa gcaactcgca atccagaaca
    35221 aataaatttt gtcattccag ctaagtagat tgtttggtta ggtaataaat tttggaaagg
    35281 aggaatgatc gtatgttgat agtaagttgt taaaaatgaa tggatatcat cttcaataac
    35341 ccatagacct tctttttcaa tcacttcggc taattgctct cgtcttttgt gagagatttg
    35401 aaaccctata ggattattac aagaaggcat aagaaagaca ccatgaatct tcttttttct
    35461 acattcctga atcaatatat caggtcgcat tccttgctcg tcaaaatcaa ttggaacgat
    35521 ctctaaatga tataactgag ccaattcaat gaaattagcg tatgtatatc ggtctgttgc
    35581 tattctatca cctggagaga aaagtgcaga aagtgtgata gccaatccat tttgaacacc
    35641 ggaaacaata gatagagatt ctaaggacgg aatgactcct tgagttttta accaatttgc
    35701 tgcagtagtc aattgaaaat ttgtgccttt gggatcttgg taattaagca aaccaacaag
    35761 atttgggtta ttactagttg attgaataaa cgggacaacg aatttattac attcttcaaa
    35821 agaactaacc agaccaaaat caatgatatg ttcggttact tcattggtag agatggtgat
    35881 ggactcaaag acattgggag aaataaatgt gccacttcca atattcgtat acagcaaacc
    35941 tttctcaatt ccatatttat aagccttgcc aacggtagta aaatttaaat ctaaataatc
    36001 agctagctct ctttgggagg gtaactgagt gtttttctgt aatttattct ttaaaatatc
    36061 ttgttctaat tgttgaatca acgatcgata ataaggacgc ttcaattttt gtttgtctgg
    36121 tcgccaagtt aacgggtaat tgtcataaga attaataggc attttctcca ctccttaaaa
    36181 ttgtatacca tacaatttta ccattgattg tattgatttt agaagttaat cttttgtgag
    36241 gagggaattg tctatgaaaa ttattaattt tgaggaagta gtagcttctt ttagttttga
    36301 agaagcgatt caagtaatga aaaaatgttt ttttgattat gaaagtggta aaatcagcca
    36361 aaatcctcga ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt ttgcgatgat
    36421 gcctgcttat ttgggggaga acagatattt tggcacaaaa gtcattaccg cttttccgga
    36481 taatcatcag tatcatttac cttctcattt aggagagatt ttcttgtttg atgctcaaaa
    36541 cggactaccc aaagcattga taaacgcaaa tgcagtcacc tggattcgaa cagctgctgt
    36601 atctgcgctg gcaacagatt acttagcaaa tccgcatgca gaaacacttg ctttaattgg
    36661 agctggtcaa caagcattgt cgcatttgac tgcgatttct accattcgtc ccatcactac
    36721 agtgtttgtt tacgatattt tagaagaaag acgaaatact tttatcgcta aagctaaaaa
    36781 acagtatcct aaaataacgt ttataaatgc aatatcagta gaagatgcag tacgtccagc
    36841 ggaaatcatt tgtacgttga cctctagtaa agaggcattt cttcaaaaag agtgggtaag
    36901 ggaagacgca catatcaatg ctgtgggcac atttactcct gatacaagag agataagtac
    36961 atcgttgatg gaaacttctc aaatctatgt agatgattac tcagctgcct taacagaaag
    37021 cggggacctg cttatagcca tggcagaagg agatttagag gaatcttgta ttgttggtac
    37081 attaggcgaa ttagtttctg agaaaaaaac attttataaa ggagaatcga ggccgacgat
    37141 tttcgacgca gttggtctag ctattgaaga tctttgttgt gcagaatata tttataacaa
    37201 attagagaaa ggggctttaa aatgaactta gattattacg tagttgatgc atttgcagat
    37261 gaggtgttca aaggaaatcc agcagcggtt tatgtgctag aagaatggct gccagaaggt
    37321 acgatgcaaa aaattgccat tgaaaacaat ttatctgaaa cagcatttac agtgaagaaa
    37381 aaccaagaat ttgagcttag atggtttacg ccagatcgag aaatcgatct ttgtggtcac
    37441 gcgacattag caacagcatt tgttttattt aactattaca aaatacctga tgaaacgatt
    37501 aaattttcta ctcaaagcgg taatctattt gtgacaagaa ttaaagatta ttattacatg
    37561 gattttccta gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga agaagcgatt
    37621 ggggctaaga ttaaagaagc ctacttagca cgtgatttat ttttcgtatt agaagatgaa
    37681 aaaacggtgg ctaaattgca gccagatttt acagctatca aaaattttga attaggaata
    37741 ggagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac cttctttcca
    37801 aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct tattccttat
    37861 tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc aaggggcggg
    37921 gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc agttttgttt
    37981 tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa ttggtggaga
    38041 gtggcaacct tatagctaca atggttgtta aaaaaagacg caaacttttt caatttaatg
    38101 aaaagtttgc gtctcatttt aattattggt gtttgttttt tattcaatca agagtaaaat
    38161 gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt caatgacaaa
    38221 gtagtaagaa ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa gaccggcagc
    38281 tttatgttgc tcgtgtaatt tttctagatc atcagctgct atagcgatat gaccatagcc
    38341 gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt catagtcatc
    38401 cccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac ggcggcggct
    38461 ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat ctttcactcg
    38521 aacacatgtg tgagccattt tcatagattc atctctcctt ttccttttac tcaatcataa
    38581 caaaaagaag taaggaaata gttgatttgt gcttgttcat gagaaatttc tcatgtaaaa
    38641 tggatagaaa ataaaactgt taaggtggaa actattcatg gataaaccaa tttttggtga
    38701 aaaaaagcaa tttgctgatt atcaaacccg ttatgcagca tatattgtca tagaaagaga
    38761 tcagcagatt gcagtaatcg aggctccaaa tggtgctttt tttcttcctg gcggggaaat
    38821 tgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag aaatgggaat
    38881 tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact cgaattatcg
    38941 tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga agcgtatagg
    39001 agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag cgcttgtgaa
    39061 attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa ataaaaactg
    39121 aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt acatattttt
    39181 tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt gctgcttctt
    39241 tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca atcatggata
    39301 agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca aggtgctgtg
    39361 cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg aagtaatagg
    39421 gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc gcaattgctg
    39481 catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg tctggtgtca
    39541 tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg tggatcgtat
    39601 aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa tggagatgac
    39661 tgccgacacg gcgtgccatc acttcgatct ctaggaaatc ttcatgtgtc agtccgtgac
    39721 gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa gtaataacgt
    39781 aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct ggtaagggac
    39841 ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc gcttctttta
    39901 cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg atagcgacta
    39961 gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat gcgccattac
    40021 ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga tcacgcaagg
    40081 acggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct tgtcccgcta
    40141 atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca tttcgcaaaa
    40201 tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa ggtagatcgt
    40261 agctagaatt cacagcagtg taataaacag gattatatcc gaccttttct actaacaaag
    40321 tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat ttcagtggtt
    40381 tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat tcagaccatt
    40441 gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct aaagtaaagc
    40501 ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg gcatcttctt
    40561 gatacaactc gtgtgcgcga tgaagcagat gctccaaaat tacttccata ccacgggaaa
    40621 caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa tcttccactg
    40681 catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc acacgtaata
    40741 ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc cgaagcaaat
    40801 agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt gggttggggt
    40861 aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact cggttaagaa
    40921 tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct tcatgattcg
    40981 tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca tgcaacagtg
    41041 ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct attttttctt
    41101 ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa acgcctaatg
    41161 aatgagtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg cctaattgtt
    41221 taatgcgtcg tagacgttgg acttctttcg aattgatcaa atctaagatt acttgatgct
    41281 ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc agcatttgat
    41341 atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc cattctctgg
    41401 atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt agaatcctgt
    41461 aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt gacttctttt
    41521 acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg atccactgca
    41581 ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg cttcatgact
    41641 tgcccgcgtt tttcctgatt accgttgaca cttaagtaca tcataacaga aatcccattt
    41701 ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa atcataagtt
    41761 ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg aaaagcgtat
    41821 ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt tgtgtcactt
    41881 tgggggaaaa tcaaagaaat attcaaaatt ccttcatctg atacgatccc taatccacct
    41941 gaatttctga tcactggagc gtaacctgca tctttcagtg tttggatccc gtcacttaga
    42001 aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg ccagaaatgt
    42061 aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa tgcaaaaggg
    42121 gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa tcgcagggtt cattcaaccg
    42181 gctcctttgt ataaattcat attttctaca atattatact ctaaaatcaa agagttgttt
    42241 tggaaaaaag gttttctgta aaaatggaca agttggttga caaaacaaaa gaaactagga
    42301 acaatgtagc tatgagaagt aaaggagaat gaataatgga attatcacaa ggaacaccga
    42361 tttcaatcaa attgcgtaca aaagtaaaac agcaagggga agtgcaagat ttttattttg
    42421 atttgaaagg gcaaatggtg acaattggcg atacgctgta catacgttat aaagagattc
    42481 aggaggagac gggcgatgag attcctgtaa cgatcaaatt agttccagat ggtcatgtac
    42541 agctgatccg tgcaggagaa atgcggatgc gtttgaaatt tggctataaa gaacggttag
    42601 aaacatccta tcgaacacca tacggtatga tccagattgc tacttttacg aaagaactgc
    42661 atgttagttt gaaagaccgt cccacaggag ggaaggttcg aatcgattat gatctgttca
    42721 tgggaccgga aaaaatcggc gaatattatt tgaccttgga ttttacagcg taaatataaa
    42781 agaaaagttt gattttttgt gtctgatttt gtatgataag gagtagactg tgaaaggacg
    42841 tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta tcaatgatcg
    42901 aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat ggatttttct gatttagcta
    42961 accagatcca aaactatctt gggaaatcag acagtgaaat tcgtgattca ctcgcacaat
    43021 tttatactga cttgaatatt gacggcagct ttatctcatt aggggataat cgttggggct
    43081 tgcgttcatg gtatgctatc gattcgatcg atgaagaagt aaaccatgga atggacgaag
    43141 aggatgaaga tactccacgt cgccgtaaac gcaagaaagt caacgccttt atcaatgatg
    43201 atgaagacgc gatcgattac aatgacgatg acccagaaga tacagatctg acagaagaag
    43261 atgacgatga tctgtttgat gacgatgatg atgaagatga agaaatcgct gcttataatt
    43321 ctgacttgca agaaatcgga gcagatgacc cgacagatga tgaagaagac ttgccaggaa
    43381 atatcgaaga agatttaagt atcatagacg aagacgatga tgatgaagat tatgaagagg
    43441 aagaattttc tgactctgaa aaagaataaa cattattttc ttacgagagg ttgtgacaga
    43501 gacgatcagc ctatcagaat aaggaggtgt gacacgattt ttgtcacacc ttcttttttg
    43561 tatataaaaa tcccaagtaa atacataaaa aataaaaaaa ttgaaacttt tagcgcacct
    43621 tgtacgtcat attaagtgag gcagaaagga gtggagaatg atttttactg gatataagaa
    43681 aaaacggcag atcgaaaaat accgtcacct gctgaatgga ctcatcgaaa ctgattatca
    43741 aaaaatgttc cgtatcacga tgaattacgt acatgataaa gaagaggcct tagatgtgat
    43801 gcaagatagt tttcataaag ccctgaacta ttttgaaaaa gaaaaagaga tcgagcattt
    43861 tagtgcatgg ttttatcgga tattgatccg cacggcatta gatagttggc gcagacaaaa
    43921 aaggacgcct gtggacttat ttgaaattca agatatcccc caacagttac ctttagaaac
    43981 ttccgttgca gagctccata tgattttaga taaaatagac agtccggaaa aagagattct
    44041 gatcctctac ttttttgaag gatttcgttt gaaagaaatt gcgactatct tggatatgaa
    44101 tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat gctttacgtc aaatcctaga
    44161 atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa tttaaaaaaa ctaaagaaaa
    44221 cttataaaga acaagcaatt cccaaagggc ttaaagagga gatccgccag cgttttatta
    44281 tcgaagagca atcatttata aaagcaaagc ggagaaagag acgtctccaa gggttcggag
    44341 tatctttggc tatcgtaatg attacaacaa gtagccttct ttttaatagt caagttcgaa
    44401 gctttgcaga agatttaccc atcttaggat cagtcattga attgatctta ggcgaacgct
    44461 ttactgatcg atctgaaaaa atcgatattc aggtaccgaa gatcaataca caaaatgaga
    44521 aagaaaataa aacaattcat ggattaaatc aaaaatactt tcgcgaagga caagcagatt
    44581 ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga tcattaccaa gttttgggag
    44641 attatcagaa aatcgtagac gataaccggt ttctcgtgat tgaaaggcag atcacccaaa
    44701 cagcagcaga tagccatgtt gaaaaaagat acgacacaat cgacaagaaa aacagtgtcc
    44761 agctctcact tccactttta ttcaaagatg acacctatct atctgttttg acaaaagaag
    44821 tcaaacggca gatggccgaa caagtcaagg aagatccatc taagtactat tggactgagc
    44881 aagatattca agaagggacg atagaaaaac caacacttgt tacgcctaca cgaagttttt
    44941 atttgaacaa agagcatcaa ttagttttga ctttttctca atacgaaatt gctcctggtt
    45001 atatgggtac accagaattt gtcattccta agtccgtgac aaaaacgatt ttggcatcgg
    45061 aagattattt ggatcattag acgagaagta gttgcgacta gcagtttcaa ttttcaagta
    45121 agtacaagta aataataaga agagaaaaag agaaagaaga gatatagtga aaagaaatta
    45181 tttagttatc agtattattg ctttatccgt gcttttgttt ttcgaaatta cttcggtggc
    45241 ctccttgatc accgaagtaa aaggacaaac acactcttcg gagcattcgt cacaatcttt
    45301 caaacaatca gaacaatcaa ccattaaaag ccaatcaact gaaacagcta acgtcaaaga
    45361 agccattcaa gaatcagcgg aaaaggtaca aagtcagtca tcacaatcaa cacaagacag
    45421 tacaaaaacg agccgagatc cggctgtcaa aagcgtcgca atcagctttg atgatggacc
    45481 aggagcgacc acaacaccgc agcttctgcg aatcttaaaa gaaaaaaacg tgcatgccac
    45541 ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa atcgtgaagc agacagctga
    45601 agcagggcac gagattggca atcacacata cgatcatcag aatctggcca ctctttctgc
    45661 ccagtcgatg acggaggaag tgacgaaagc agacactgag atcaaaaaag tcactggcaa
    45721 aacccccact tttgttcgtc cgccctatgg ttctgtcacg aatgtaggag caacgatcat
    45781 ccagcgaccg atcatcgaat ggtctgttga ttcagaagat tggaagacaa gaaatcctga
    45841 tttgattttg cagaagatcc aagcaacagt atacgatggg gcaattattt tattccatga
    45901 tatctatcct gaaacgattc gtgccgttcc tcaagtgatc gattatctga aagagcaagg
    45961 gtatcgcatc acgacagttg gggatttatt agggcatcca acggctgtcg agaactatta
    46021 tggcagaaac gatcatcggc cagttcagta agtgagacaa gtatctttaa taaataacac
    46081 attttaataa gtagttgaac acataactac cttacttgca aaggcttaac aatatctata
    46141 caggtacaac aaaaaactgt ccaccaattt tctttggcgg acagttttca cttaaaaata
    46201 aagaagcaac tattcgtaaa ttatgctaaa atagcagtag aacgcgtcct gagggaatcg
    46261 aacccccgtc tcaagaaccg gaatcttacg tgatatccac tacactaagg acgcaagcac
    46321 tgggataatt gtaactaaac agataaaaaa ttacaagggg attggacaaa aaaatgaaat
    46381 ttgagcaaaa tttttcacaa aaacagcagc agacacagaa actagccatg acgcaacagt
    46441 tgcaacagtc catccaagtt ttacaatata acagtgagga gctgttggca ttcgttgaga
    46501 atcaagcaat ggaaaatcca ttggtggaag ttgttgagcc ggaatggcag ccagattaca
    46561 taaaagcttc ttcatcttct tatgaaggag aagagacaaa ttacctaaat cagattcctg
    46621 atacaaaagg gtctttattt gattcattga tcgaacaagt ccatttgaat taccgagata
    46681 cttttttgag gaaaatcgtt ctttatcttg tagaatacat cgatttgaac ggatttttga
    46741 cgattagttt agaagaagcc ggaaaagaga ttggagctac gcctatccaa atgctcgatg
    46801 cgttgactct gattcagcaa ttagaaccag ctggagtagg tgcacgtagt ttgcaagaat
    46861 gtctcatgct tcaaacagaa cgagatgatt atgcgccgga attagcttat atcgtgttgg
    46921 aagaatgttt tgaggaactg gtggagcgaa aatggaaaga aatcgcacaa cgtttttctg
    46981 ttgatctaca tgctgttcag cagatattcg actatttaca aacgttgagt ccaagccctg
    47041 gccggatttt cgaccgtagc agcgaattgt acattattcc tgatgtccgg gtattagtgg
    47101 acgacgataa aaatgtacaa gttatctcaa accgaaaaaa tcagccaaat atacgctttc
    47161 aagagagtta cttcaaacaa atgtcacaac aagcagataa agaaactgaa aattatttaa
    47221 aagaacggaa acaggaattc gaatggttga agaaaacgat cctacaacgt ggggatacaa
    47281 ttttgcgggt tgctcaagtc attgtttccc ggcaaaaagc tttctttatc gacaaggaac
    47341 gtccaatcaa gcctcttacg ttaaaggaag ttgcaactga gatagatgtg catgaatcca
    47401 cggtcagtcg tgcagtcaac gggaaatatt tggaaacaga cttcggtgta tttgaattaa
    47461 aaaaattttt cactacacga attcctacga atagtacaga acagacagaa gacctttcag
    47521 cagatacagc gaagaaaaag ctgcaggaat tagtcgatca agaagacaaa aacaagccgt
    47581 tgtctgacca gaaattggtt gaattgctga aaaaagatga gatagcgatc tcaagacgaa
    47641 cagtagcaaa atatcgtgat ttattaggaa tcccgagttc atcaaaaaga aaacgttatg
    47701 ataactaatc agtcaaaaga ctagaaaaag tatttcactt tttctagtct ttttagttga
    47761 aaaacgagag caaaactgtt agacttacag tcgtgaggtt gagatttccc tttattgatg
    47821 tgaaattcga atagttaatt tcttttttta tcgtatatgg gtcgttttaa gtctacattg
    47881 gacgtaaagt gtccaactaa ggagatcgct atgctggatg aaataaaaat gattgaatct
    47941 gttgcaccag atattatcga agtgttgcaa gaacgctata aaattttacg aaatatctat
    48001 tggatgcagc cgattggacg tcgaagttta tcggaaagta tggggatcac agaacgagta
    48061 ttgcgaacag aaacagatct tttgaaaaat ctgaatttga tcgacacctc aaaaagcggt
    48121 atgacgctga caactaaggg cgaagaagtt tatcaaagcc ttgaaaactt catggatcag
    48181 cttttaggta cgcatcaaac cgaacaacag ttggcagaat attttggtaa ccaacgctgt
    48241 atcgttgttt caggaaacag cgaagagcag accaaagtgg ctgatgcatt cggcgaagcg
    48301 ctgagcgaag ccctagaccg actacttcca gaaggcgaga atatcatcgc tgtgatggga
    48361 ggaactacta tggctgtggt agccgaacaa ttgtctaatc tggaaaataa gaaacggcat
    48421 aacctgtttg tccctgcaag aggcgggatt ggtgaagcga taactgtaca ggcaaattct
    48481 gtcagtgcaa gaatggctgc taaagcaaac ggtaaccacc gtgcacttta tgttccggaa
    48541 caattaagtc tggcaactta caattcttta ctcaatgagc catctatcca agaagtctta
    48601 aacttgatta gtgaagcgaa ttgtgttatt catagtattg ggcgtgcatt acacatggca
    48661 gcacgtcgta agatgactga aaaagaatta gtcatgctga aacaagcaaa tgctgttgcg
    48721 gaatcttttg gctacttttt caatgaaaaa ggtgaagtcg tttataaggt ccctagaatc
    48781 ggcatagaat tgcaagattt ggaaaaagtc ccgatcatca tggcaatcgc gggcggaaaa
    48841 tcaaaagcca aagcaattcg agcttatatg aaaaatgcac caaaacaaac gtggctcatc
    48901 actgatgaag ctgctgcaaa tgagatttta aaaggggtaa ccctttaaaa taaatatttt
    48961 tttgattttc ataaggagga aatcttaaat gacagttaaa gtaggtatta atggttttgg
    49021 acgtattgga cgtctagcat tccgtcgtat ccaagatgta gatggaatcg aagtagtagc
    49081 aatcaacgac ttgacagatg ctaaaatgtt ggcacacttg ttaaaatacg acacaactca
    49141 aggacgtttc aacggaacag ttgaagttca tgaaggttca ttcaacgtaa acggaaaaga
    49201 agttaaagta ttagctaacc gcaacccaga agaattacca tggggcgaac taggcgtaga
    49261 tatcgttcta gaatgtactg gtttcttcac ttctaaatca gcagctgaaa aacatttaac
    49321 agctggtgct aaacgcgttg ttatctctgc tcctggcgga aacgatgttc caacaatcgt
    49381 ttataacaca aaccacgaaa cattaactgg taaagaaaca gttatctcag gtgcttcatg
    49441 tactactaac tgtttagctc ctatggctaa aacattgaac gacaaatttg gtgttgttga
    49501 aggattaatg acaactatcc acgcttacac aggtgaccaa atgactctag acggacctca
    49561 tcctaaaggt gacttccgcc gtgcacgcgc tgctgctgca aacatcgttc ctaactcaac
    49621 aggtgctgct aaagctatcg gtttagtaat tcctgaattg aacggtaaat tagacggagc
    49681 tgctcaacgt gttcctgtac caacaggttc attaacagaa ttagtaacag ttcttgaaaa
    49741 agaagtaact gttgacgaaa tcaatgcagc aatgaaagaa gcttcaaacg aatcttacgg
    49801 atacaacaca gacgaaatcg tttcttcaga tatcgttggt atgacttacg gttcattatt
    49861 tgatgctaca caaactaaag taatgacagt tggcgacaaa caattagtta aaactgttgc
    49921 ttggtacgac aacgaaatgt catacactgc acaattagta cgtactttag aatacttcgc
    49981 taacttataa gatagacaaa cgtacaagtg taaattatag acatgtacat ttaaaagcgg
    50041 ggaagcaatc gcgcttcttc cgcttttttt gataataagt ttattcagga ggaaataaaa
    50101 tggctaagaa aacagtgaaa gatatcgatt taaaagacaa aaaagttctt gtccgtgttg
    50161 acttcaacgt tcctttgaaa gacggcgtga tcactgacga cactcgtatc aaagcagcat
    50221 tgccaacaat caactacgtt ctagaacaag gcggaaaagc aatccttttc tctcaccttg
    50281 gacgtgtaaa aacagaagaa gataaagaag gaaaatcttt agctccagtt gcaaaacgtc
    50341 taggcgaatt attaggtaaa gaagtaacat tcgtacctga aactcgcgga gaacaattag
    50401 aagaagcaat ccgcaacatg aatgacggcg acgttgtcgt attcgaaaat acacgatttg
    50461 aagatattga cggtaaaaaa gaaagcaaaa atgatacaga actaggtaaa tactgggctt
    50521 cattaggcga tgtatttgtt aacgatgctt tcggtacagc tcaccgtgca catgcttcta
    50581 acgtaggtat cgcttcaact ggtatcccaa cagttgctgg attcttgatg gaaaaagaaa
    50641 tcaagttcat cggtgaagca gtagaagaac caaaacgacc aatgattgca atccttggtg
    50701 gcgcaaaagt ttctgataaa atcggcgtaa tcgaaaactt gattcctaaa gcagataaaa
    50761 tcttgatcgg tggcggaatg acttacacat tctacgaagc aaaaggaatc aagatcggta
    50821 actctctagt agaagctgat aaagtggaac tagcaaaaga attaatcgaa aaagcaggcg
    50881 acaaacttgt cttgccaatc gacaatgtct gtgcaccaga attttcaaat gatgttgaaa
    50941 cacaagtaat cgaaggagac attcctgatg gtttgatggc tttagacatc ggcccagcat
    51001 cagtaaaatt atttgctgat acattacaag gtgcaaaaac agttgtttgg aacggaccta
    51061 tgggtgtatt tgaaatgagt aactttgcta aaggtacaat cggtgtttgt gaagcaatcg
    51121 ctaacttaga aggcgcaaca acaatcatcg gtggcggaga ctcagccgca gcagcagaac
    51181 aattaggatt tgctgacaaa ttcacacaca tctcaacagg tggcggcgca agcttggaat
    51241 tgcttgaagg taaagaatta ccaggactag cagcaatcaa cgataaataa acgaccaagt
    51301 ttgctcaatt ctgacgagta atcgatcaaa aggtgaaggg agttttcatg actccctagc
    51361 catttttcaa taaattaagg agtgtgttcc tcaatgcgta aacctattat cgcaggtaac
    51421 tggaaaatga acaaaactgc atcagaagca aaagaatttg ctgaagcagt aaaaaacaaa
    51481 atcccttcaa atgacaaagt tgattcagtc atcggatctc ctgcattatt cttacaagaa
    51541 ttaatgacat cagctgaagg tacagaacta aaaatctcag cacaaaactg ctactgggaa
    51601 aactcaggtg cgttcactgg tgaaacttca ccagcagctc ttgctgatct aggtgttcat
    51661 tatgtgatca tcggacattc agaacgtcgt gaatatttcc atgaaacaga cgaagacatc
    51721 aataaaaaag cgaaagctat ctttgcaaat ggtatgacac caatcctatg ctgtggcgaa
    51781 tctttagaaa cctatgaagc aggcaaaact gctgaatgga tcgaaggaca aattacagct
    51841 ggtttagctg atctttcagc tgaacaagtg tcaaacatgg ttattgctta tgaaccaatc
    51901 tgggcaatcg gtactggtaa atcagctgac gcacaaatcg ctgacgaaat ttgtggagtt
    51961 gtacgtcaaa cagttgaaaa actttatgga aaagaagtat cagaagcagt tcgtatccaa
    52021 tacggcggtt ctgtaaaacc agaaaatatt gctgaataca tggcaaaaga aaatgttgac
    52081 ggtgctttag tcggtggcgc aagcttgcaa gcagattcat tcttgtcatt gttagacgct
    52141 gtaaaataat tgttgaaaat ttgcgaaaaa tgcaagaaat gtttgcacta atgagcaaaa
    52201 ttaactaaaa tcgtatatga gggaaatccc ttaggaaaaa acaaactcaa aggagagaca
    52261 aaacatgtca attattactg atgtttacgc tcgcgagatc ttagactcac gtggtaaccc
    52321 aacaatcgaa gtagaagtat acacagaaag cggagctttt ggccgcggaa tggttccttc
    52381 aggtgcttca actggtgaat acgaagcagt tgaattacgt gacggagaca aagctcgtta
    52441 tggcggtaaa ggtgtaacta aagctgtcga caacgtaaac aacatcattg ctgaagcaat
    52501 cattggctac gatgttcgtg accaaatggc tatcgacaaa gctatgatcg ctttagacgg
    52561 aactcctaac aaaggtaaat taggtgcgaa cgctatttta ggtgtttcta tcgctgttgc
    52621 acgtgcagct gctgactacc tagaagtacc tttataccac tacctaggcg gattcaacac
    52681 aaaagtattg ccaacaccaa tgatgaacat catcaacggc ggatcacatg ctgataactc
    52741 aatcgacttc caagaattca tgatcatgcc tgttggcgct ccaacattca aagaagctct
    52801 acgtatgggt gctgaagtat tccacgcatt agcttcaatc ttgaaagctc gcggattagc
    52861 aacttctgtt ggtgacgaag gtggatttgc tccaaacctt ggttcaaacg aagaaggttt
    52921 tgaagtaatc atcgaagcaa tcgaaaaagc tggctatgta cctggtaaag acgttgttct
    52981 tgctatggat gctgcttctt cagaattcta cgacaaagaa aaaggtgttt acgtactagc
    53041 tgattcaggc gaaggcgaaa aaacaactga cgaaatgatc aaattctacg aagaattagt
    53101 ttctaaatat ccaatcatct caatcgaaga cggattagac gaaaacgact gggacggatt
    53161 caagaaatta actgacgtat taggcgacaa agttcaatta gttggtgacg acttgttcgt
    53221 aacaaacact caaaaattat ctgaaggtat cgaaaaagga attgctaact caatccttat
    53281 caaagtgaac caaatcggta cattaacaga aactttcgaa gctatcgaaa tggctaaaga
    53341 agctggctac acagcagttg tatctcaccg ttctggtgaa acagaagatt caacaatctc
    53401 tgatatcgct gttgcaacaa acgctggcca aatcaaaact ggttctctat cacgtactga
    53461 ccgtattgct aaatacaacc aattactacg tatcgaagac caacttggtg aagttgcaga
    53521 atacaaaggt ttgaaatctt tctacaactt aaaaaacaaa taattctttt ctaatgaaga
    53581 tacatgatcg actattgaaa gatagtcgag tatgatcact tagttaagta atcgaactct
    53641 ctacttgctg aaatatcagc aggtagagag ttttttttgc aactagcttc tgtatatgat
    53701 gtttacaaag aatggtacaa gaaaagatca tccttgaaaa aggaaagcta acaataacta
    53761 gcatcatata agagctttta aggatagaac agtagaatag gaatctatga gcaagtcctc
    53821 tcgtataaat aaaataggag ggaagtagca gcatattttt cataaagtgg ctctctttct
    53881 tcatttttac tttggttgct cctatttgaa aattcgagca cactcaaata caataaaaag
    53941 aaatggcaag ggagaggatt agatgaaaaa agaagaaaaa atcgcgattt tacaagagat
    54001 catccgaatt aaatcagtga atggaaacga aggtgaagta gcagcctatt tgaataagct
    54061 tttggcaaag catgacataa caggagagat tgtttcttac agagatggta gagataattt
    54121 gattgctcgt taccaaaagg gccaatcagg aaaagtgtta ggcctatctg ggcatatgga
    54181 tgtcgtggcg gctggtgatg aatcgtcttg gacctatgca ccctttgcag cagaaatcca
    54241 tgggaaccgt ttgtacggac gtggtgctac agatatgaag agtggcttgg cagcaatggt
    54301 gattgccatg atcgaactga aagagtcagg aaaaccattt aacggaacag tcaaattatt
    54361 agccactgtc ggagaagaag tgggtgaact aggtggggaa cagctcacga aagcaggcta
    54421 tgtagatgac ttagatgcct tgatcatcgg agagccaact aattacagtt tgatgtatac
    54481 gcacatggga tcaatcaact atacagtcac ctcgcacgga aaagaagcgc atagctctat
    54541 gccagaccaa ggttataatg caatcaatca cctaaatgaa ttcattacga aagcaaatgc
    54601 tgaaatgaat cacctcgctg aaacaatcga gaatcctgtc ctaggaaaaa caatccataa
    54661 tgttacattg atcagtggcg ggaatcaagt caacagcatc ccaagccatg cgcagctcca
    54721 agggaacatt cgatcgattc cagaataccc aaatgataaa atcattgcat tgctgcaatc
    54781 aatcgtcaat gaattgaatc aagagacaga ctatcatttg gaattgatga tcgattacaa
    54841 caaaatacct gtcaaagctg atccagattc tcctttgatt cattgcattc agcaacaatt
    54901 cagccagcca ttacctttag ttggagcggc agcaacaacg gatgcagccg aatttacgaa
    54961 agctaaccat tccttcgact ttgtcgtctt tgggcctggt gtcgttactt tgcctcatca
    55021 agtagatgag tatgttgaaa tcgataatta tttggatatg attgaaaaat atcaaggaat
    55081 tattttatcc tacttagcgt aagatttttt taataaatga aaaaacagag ccagattagc
    55141 attattttgc taatccagct ctgtttattc agcctatttt tctttttgaa tatatcaagc
    55201 gttgtggcaa catagtgatg actaatgaaa caacgacgat agacaacaaa cgatcaaggt
    55261 agtctgtacc tgcctgtacc aagatcacac ttgttgtctg acttaaacct aacccatgta
    55321 atagctgaac gatcatactg gatcctgagg aggtgatccc gccaaataac tgaacggtaa
    55381 tgatcgaaga aacgatcgtt ccaggtaccg acaaaatcaa agcgagtaat aacagtttca
    55441 agctgttttt atctttgccg agatatcttg gcaaaatcaa tccagcaagc aagcctgtga
    55501 gaatttggat tggcgaataa tacagagaaa aaatatctgt agtcactcca cttaaaatac
    55561 cgctggacaa tcctgtcagc attccaggta caggcccaag caatgcacca gcaaaaatcg
    55621 ttccgataga atcgagatag attggcaaac gaagccataa agcaatcgtt cctccaatat
    55681 aattgatccc aatacacaaa gccatcactg taagtacgcg agttgccatt ttgtttttat
    55741 tcattttgtt agcccatctt taacctttcc aaatctttca taatcgtttc ttcttgtgca
    55801 tttaaaagaa cggttaaaaa ctttcggaaa aaaagacgag tatctacttt tgtcataatt
    55861 ttactgttag caggtttctg ccaaaattca taacgatcaa ccaatgtttg tccacgactt
    55921 attccttgag tttccacagc agtgaagctt ttgaaacctt cacagatggc cccctcgata
    55981 aaataagcaa cagctaacgg atcattgatg acacatccta aaattctttc ttgtttccag
    56041 tggaagtcaa aataaaaacg agtgatcgct tttagatact ctccctcttc aggattcatt
    56101 tgacagcagt attcgaggat agtcggtgtc aagacgattt ctcttgtcac atctaaccca
    56161 accatttcta tcgtttgttt gagattctcg aatacataag aagctgcttc aggatcacac
    56221 cagtaattgt attcagcaac tggcgaacag ttaccatggc ttttgtaagt tccacccatc
    56281 gagacaaaac gatccatatg tttcccaata tttgggttga ctttcaaagc aaaagcgata
    56341 ttggtcaaag gacccaaagc aatgactgat gtatttgtct tttctttgaa ataatcagcc
    56401 aaaaaatcaa cggcatggag aggttctgcc tgttttctca agatcatcgg gaaatttgtt
    56461 tcccctaaac catccatacc atgagtgtct tgtgcactga tgaaaggttg cttcaaaggt
    56521 ttttccattc cttgataaac aggaatgtct aaacgatcac agcgttccag gcatttcagc
    56581 acattctgcg tacctatatc tgcagggaca ttcccacaaa ctacggtaat agcaactact
    56641 tcgatatcag gggatttgac agctaatgat aaagctaaag tgtcgtcgat ccctggatcg
    56701 caatcaataa ttacttttcg cattatttcc aatctccttt ctttagagat acttagtttt
    56761 ttattctggg aagtttccga accagtccaa gacgagcgtc ttgattataa tgcacgaaat
    56821 tagtatactg gcttttattg cgaatgtaaa tgctctctta caagtaaatc tattgaggag
    56881 aactttaagt aaatgataca atgaaaaaga aatggaattt acgtgtgccg aaaataaaga
    56941 atcctatgga tcggtacatt ggataatagt tagattatag tttggcagaa agcggcgggt
    57001 gtacaatggt tattttttat ttcattctta ttctgataat tatagggatc agttaccatt
    57061 ttctttctta tttttatgca aagaaattag taagagcggg agtacagaga ggatatgact
    57121 ggtatgaaga cactggtcat cgtttgatgg accctgctgc agtcacgtca gtagaaaaaa
    57181 aacgacaaaa attagaagaa gagtgtgaaa gcttttggga gcagggaaca cccaaaacaa
    57241 tcaaaagtta tgacgggttg aaactagctg gtcagatgtt catccatcca aataagcaga
    57301 ataaatgggt catttgcgta catgattacc gaagcacagg gaaaagagac atgtctcata
    57361 taggtaaaag atatgcagag aaaggattca acgttcttgt acctgattta cgtgcgcacg
    57421 gagaaagtga aggagaaatc atcggaatgg gatggctaga tcggctagat ttaattgcct
    57481 ggatccaact catcttagat gaacagccga atgcgtccat tattttacac ggcggatcaa
    57541 tgggagcttc tacgatcatg atggcaagtg gtgaaaaact tccatcagcg gtcaaaggat
    57601 ttattttaga cagcggatat gtttctgttt atgcagaatt ccgatatatg ttaagtaaaa
    57661 tcactgtctt tcctaaaaaa atggtcatga gatatgcgaa tcattatgcc cagaaatacg
    57721 ccggttactc cttaaagcaa gcatcagcta cgagacagct tggcagcaac catctgcctt
    57781 tattggtcat tcatggtgaa agagatcatt ttgtccctac agaagcagcg tacacgattc
    57841 aaaatgctac ggcaggggat aaagcactgc tgcttgttcc agaagcagaa cacttggaag
    57901 catcgatgaa agatccgaaa acctattgga cagtgatttt ttcatttatt gaacaaagaa
    57961 taacgatgaa ttaattttct cttctttttt gtaagtaatt cgaggataaa taattgttta
    58021 tagatcagga gactgctaaa ataaggtagg aataaaaaaa tatcagttat ttgaggagtg
    58081 cggcatatgg aaatcaaaga agaaaaaaat cgtcttgctt tattcaatga tgaacaacaa
    58141 gagatcggag aaatgacatg gtcagatgcg ggcccagaca tcatgatcat tgaccataca
    58201 tttgtagatc cagcatatcg tggtcaaaag ttagctgaaa agctagtgta tactggagta
    58261 gaattagcaa gacgagacgg gaaaaagatc attcctttat gcccttatgc aaaaaaagag
    58321 tttgaaaaga aaccagaata tcaagacgtc ttacgtcagt cataagaata acagctgaaa
    58381 gttttgaact ttcagctgtt attccattta tatagagttg tcacggactt ttatctatga
    58441 taaagttaaa gagacgatat cttttgggaa ggaggctgcg atatgtataa tttaattttg
    58501 ggaatcgtga tcgttttatc gattgttatg gtcattgcga ttatgatgca gccaagcaag
    58561 caaaatagtg cagcgagtgc atttaccggc ggtgcggatc aactattcgg caaacaaaaa
    58621 gctcgcggct ttgaagctgt catgcagcga tcaactgcag ttatgggagc tatctggatg
    58681 atcttattat ttgtcctagc atatttatct tcgaagtagt tgaataagcc tctcgcaaaa
    58741 cggcgggcgg ctttttttat tgcaaagaga acgcttaaga agaagttatg aaagaaacta
    58801 tggtaaaatg aggcgaaaga ggtgagacga gtgagacaat taccaaaacc cctttatgca
    58861 aaacatggaa agcgagcggt tttgctgctt cacgcttact caggaagtcc aaacgatgtt
    58921 cgaatgctcg cacgattttt ggaaaaagca gattatacag tttatgcgcc gctttttaaa
    58981 ggacacggaa caatggagcc atatgatatt ttgcaagaaa aagcagaaag ctggtgggca
    59041 gacactaaaa aagccatcca ttttttacag tctgagcagt tttctgatat tgccgtatta
    59101 ggattatcaa tgggcggcat ctttgctgta cgagcactag aagaagaatc agtgattggc
    59161 ggcggttttt tctgttcgcc tttgtctcct gtaaaaacaa acgtgcctga aaactttgaa
    59221 aaatacgtac gtcaagtgct gaagacagca ggtaaatcag agaaagaaat caatgaaaaa
    59281 gcagttgctt accgctcgtt agctgaacaa caattaatgg atatacaaga tcaggcagca
    59341 atcgtagaaa gtaggttgtc agatatccaa cagccgatat ttttagctca agctgggaaa
    59401 gatgaaatga tcgaccctaa tggcgtattt gaaactgcac gcaagttgag ccgtcagcga
    59461 gtgacacttc gatggtatcc tgaaagtggt catgtgatta cagttggtac agcaagacgc
    59521 gaattagaaa aagacgtctt ggaattttta gaaaaattac cttggaatga ggaataaata
    59581 tgacaaaaga aacgataaaa gagaaaattc tctttttcat ggaaagcagt cgcaaacgca
    59641 gtttttctat ggaagaaatc gctgaaggtt tagggttcca aaaaagcgat gattttaaat
    59701 tactagtaca aacgattgct caaatggaaa gagaacagag tatagttttc aataaaaaag
    59761 gaaaagtgaa acttccttta aaaccaatct tgatcgaagg aacatttcga gcaaatgaac
    59821 gaggatttgg atttgtaacg attgatcccg aagaagatga catctatatc ccaaaagaag
    59881 cgactggttt tgcaatggat ggagacaccg tagcaatcga tatcatgaag cgagcaaata
    59941 cagatatgga tcgcggagca gaggggaaag tagtcgagat tcgagaccgg gcgactactc
    60001 agttagcagg ggaattcgtt gcttattcag aagaagaaat acaagaaacg gatctttacg
    60061 gcgttgttat cccgaaagat agaaagctca accaatttaa agtatatgca gcagcagagg
    60121 gagttcgtcc agttgacgga agtattgtgc tgatcgaact gacccattac cctgaaaaag
    60181 ggtatgcgac tagtttagaa gggataatca agcaagtgat tggtcataag aatgaccctg
    60241 gcatggatat cctctcaatt gtcgtggcta atggtatccc tacgaaattt cctgatgatg
    60301 tattgtctga agcagatgaa gttccagatc aaatcagcga aaaggatata actggacgta
    60361 gagatttaag aaatcagctc attgttacga ttgatggaga agatgcaaaa gatctagatg
    60421 atgcagttac ggttaaaaaa ttagataacg gtaattattt tcttggagta cacattgcag
    60481 atgtttctta ctatgtgaca caaggcagtc aattggacag agaagcatat gaaagaggga
    60541 caagtgtata tttgactgat cgagtgattc cgatgatccc gcaacgttta tcgaatggta
    60601 tctgttcact gaatccccat gttccaaggc tgaccatgag ttgcgaaatg gagattgatc
    60661 caaatggaca agtagtctct catgatattt tcccaagtgt gatccaaaca acagaacgga
    60721 tgacctacac agctgtcaat caaatcttag aagatcaaga cgaagaaacg atgcaacgct
    60781 atgctcagct cgtacctatg ttccaagaaa tgggtgaatt gcatcgaatc cttgaaacga
    60841 tgcgaatcaa acgtggcgcg atttcttttg aggatcgcga agcaaaaatc cttgttgatg
    60901 ctgaaggaca cccaaatgat atccagctaa gaagcagagg aattggtgaa cgcttgatcg
    60961 aatctttcat gcttgcggcc aatgaaacgg tagctgaaca ttataataaa ttaaatctgc
    61021 cgtttattta ccgaatccat gaacagccaa aagaagaaaa aatgcagcgc ttctttgatt
    61081 ttgcttcagc tttgggcatt ctggtccgag gaacgaaagg aacgatcaca ccaaaagatt
    61141 tacagcatgt gatcgaagga gtagaagaca aaccagaagc tgcagttatc aatactatgt
    61201 tgctaagaag catgcaacaa gcacgctatt cagaagacaa ttttgggcat tatggcttag
    61261 cggcagatta ctacacacac tttacatctc ctattcgacg ctatcccgat ctaatcgtcc
    61321 atcgtctgat ccgttcttac agtgaagata aaagtgaaag caatcaaagt tcgtgggcta
    61381 cggaacttcc tgaaattgca gatcatagtt ctaagatgga acgtcgtgca gtagaaacag
    61441 aacgtgaagt agattccatg aagaaagcag aattcatgat ggacaaagtg ggggaagaat
    61501 tcgatgggat catcagctct gttgtgaaat tcggcctgtt tatcgaactg ccgaatacag
    61561 tagagggatt gatccatatc aatgaattga aacaggacta cttccatttt gttgaaaatc
    61621 atttagctct tgtaggtgaa agaactggtc tgacttttaa aatcggacag aaagtgcgag
    61681 taaaagtagt caaagctgat ccagatgaac gagcagtaga ttttgaactt gtttccgcag
    61741 aagaggttac agcgttggaa cggccaaaaa cacgaaatgg aaaatcagga aaacgcgaag
    61801 gaaaacaagc aggaaaacgc ccagaaagaa atggcaagaa gaacaaacga tatagcgata
    61861 gtaagcaaac gttcggtaag aaaaaaggga agaagccatt ttacaaaggt gttaaaaaga
    61921 agaaaaagaa aaaataaaat gagttggagg aaacagctat gccaaaaggc gagggaaaat
    61981 taattgcaca aaacaagaaa gctcgccatg attattcgat catcgacacg atggaagcag
    62041 ggatggtctt gcaaggaacc gagatcaagt cgatacgaaa cagccggatc aatctaaaag
    62101 atggatttat tcgcgtccgc aacggagaag ctttcttgca taatgttcat atcagtcctt
    62161 atgaacaagg aaatattttt aatcatgatc cgttgcgcac gagaaagtta ttattacaca
    62221 aaaaacaaat catccggctt gaaaatgaat tgaaaaatac tggaatcact gttgttcctt
    62281 taaaagtcta tattcgtaac ggctatgcca aggtattgat tggtctggcg aaagggaaaa
    62341 aatcttatga taaacgggaa gatttgaaac gaaaagatat cgatcgacaa attgatcgaa
    62401 cattaaaaaa tttctctaga taatttcatt atcctttttc gcatattcgg tgcttttgct
    62461 tttacttaat gataaaattt gttagcattt aatctgataa aacatgatta tcataggttt
    62521 tttcttagat caggagcacc gtgtataaag aaaaattcgc ttcgtttttg actgaaataa
    62581 aaaagtgtct aattttcatg aaaatgtttt gaattttctg tttcatggtg gtatgatacc
    62641 gtgtggttag aaaccgattg gaaaaaacca gatacgcaca tgaattgatt ttatgtgaat
    62701 ttcggggaaa agaggtgaaa tggattggat gaacgctcgc taacgttcca cattggacca
    62761 atttggtttg atggtacagt ttgtataatg gtgttactga cctgtttgat tgttttcttc
    62821 ttagcttact tttttacaag aaatcttaag ttgaaaccaa caggcaaaca aaatgcgata
    62881 gaatgggtca ttgattttag tcgaggaatc gtaacagata atctgcctag aaaagaattg
    62941 agcaatttcc atttgttggc atttacattg tttttatttg tttttgttgc aaacaacatc
    63001 ggtttagtaa caaaaatcgt tttacctaac gaaacaacac tttggaaaag cccgacagca
    63061 gacccgtttg ttactttaac tttggcattt ataatgatct tacttactca cttatttggg
    63121 gtgaaaaagt taggcttcaa aggatatttc gtaaattcgt tcttgaaacc atatagtttt
    63181 atgtttccga tgaaactgat tgaagaattt acaaacttat taacacttgc tttacgtctt
    63241 tatgggaata tttatgcggg ggaagttttg ctaaccttga ttgcaaaaat ggtaataagc
    63301 ttaggctggt tctcattgcc gttagcaatt ccattagaga tggtctggat cgcattctcc
    63361 ttgtttatcg gaagcatcca agcatttgtc ttcgttactt tatctatggt ttatatgagc
    63421 cataagattg aagtggaaga ataatattcg ttttaaaaac aaactactat ttatctaatt
    63481 taggaggaaa acaattatga attatatcgc agcagcaatc gcaatcatgg gagcagcaat
    63541 cggagccggt tatggtaacg gtcaagttat ctctaaaaca atcgaatcaa tggctcgcca
    63601 acctgaaatg tctggtcaat tccgtacaac aatgtttatc ggggtagctc tagtcgaagc
    63661 tgttcctatc ttaggggtag ttgtcgcctt gattctagtt tttggtgttt aattgaatca
    63721 gtattaaggc agaaacaatt tggtttgtta ctgctttttt acatgaaaac tagaagtact
    63781 tcaaagaaag gaaggctcgc ctatgctgaa tcaattggca attgcagaag ttggcaatgc
    63841 gatgttagga aatgtcatcg ttgtcagcgg ctcatttttg atactactgg ctctcttgaa
    63901 gcattttgct tggggaccaa tcagcgatat tttgaaaaaa cgtgaagaca agatcgccaa
    63961 tgatttggat tctgcagaac aatctcgcat caactcagcg aaaatggaac aagaacgcga
    64021 acaacaattg ttagcctctc gttctgatgc agctgatatc atcaaaaatg cgaaagaaag
    64081 tggagaatta agccgccaaa atattttgaa ggatgctcaa gaagaagcag ctcgtctaaa
    64141 aagtaaagcc caagctgata tcactgtaga acgtgattca gcgctgaact ctgtaaaaga
    64201 cgatgttgca gaactatctc ttcaaatcgc ggaaaaaatc ttgaacaaag aattatctcc
    64261 agaaatgcac gaatcattaa tcaatcaata tatcgaaggt ctaggttctt caaatgaaac
    64321 tagataaata tacagtagga agacgttacg ggaaagcttt atttgaactg gcgatcgatt
    64381 caaataaagc agaagaaatt tatcaagatc tcgtaagctt acgtcaaatc tatgaacaag
    64441 tacctggatt aggtaatatg ttaagtgatg ttcgtctaga acctcatgaa aaacgagaaa
    64501 tcatggacaa gcttgttagt ggctatgatg gaatcgttaa aaacttttta gaagttgttt
    64561 atgcgtataa ccgaatggat gatcttttgt tcatgatcga agaatatgaa cgtcgatatg
    64621 atgaacatca aggtttgctg ttgggaagtg tcacaacagc agttccactt tcagacgaac
    64681 aacgaataca attagaaaag aatgttgcaa aaacaatgaa ctatcaatca gtcgaattaa
    64741 aacaaatcgt tgattcttct attattggtg gcgcgattgt tgaagctaac catcgagtaa
    64801 tcgacggaag cattcgcacg caattagaaa atatgcgtaa tcaattaaat agatagtaca
    64861 aagaggtagg tgaatcgaat ggccatcaaa gcagaagaaa ttagtgcctt gattaaagaa
    64921 caaattgaaa attatcaaaa cgtgctttca gtcgaagaaa tcggtaccgt aacttatgta
    64981 ggtgacggga tcgctcgtgc acatggttta gaaaacgcga tgagtggtga gttgcttgaa
    65041 ttttcaaatg gctcatacgg aatggcgcaa aacttagaat caaatgatgt aggtatcatt
    65101 atccttggcg atttcgagtc cattcgtgaa ggagataaag taaaacgaac aggaaagatc
    65161 atggaagttc cagttggaga ggccttgatt ggacgggtag ttaatccgct aggtcagcca
    65221 attgacggac taggtgaaat cgttactgac aaagctcgtc cagtagaagc aatggcacca
    65281 ggcgttatgc aacgtaaatc tgttaacgaa ccaatgcaaa caggtctaaa agcaatcgac
    65341 gctcttgtac caatcggacg cggccaacgt gaattagtta tcggtgaccg taaaacaggg
    65401 aaaacttcca ttgcgatcga tacgatcatc aaccaaaaag gtcaagatat gatctgtatc
    65461 tatgtagcaa tcggacaaaa agattctaca gttcgtacac aagttgaaac attgaaaaaa
    65521 tatggcgcaa tggattacac aatcgttgtg aatgccggtg cgtctcaacc agcgccgttg
    65581 ctttatatcg caccatatgc tggtactgca atgggtgaag agttcatgta caacgggaaa
    65641 catgtcttga tcatttttga tgatctttca aaacaagctg ttgcttatcg tgaactttcc
    65701 ttgctgcttc gccgtccacc aggtcgtgaa gcttatccag gggatgtttt ctacttacac
    65761 tcacgcttat tggaacgtgc agcaaaatta agtgatgatt taggtggcgg ttcaatgact
    65821 gccttaccat ttgttgaaac acaagctgga gatatctctg cttatatccc aacaaacgtt
    65881 atttcgatca ctgatggaca aatcttctta gaaagcgact tgttctacgc aggtactcgt
    65941 ccagcggttg atgccggact atctgtatct cgtgtagggg gttctgcgca aatcaaagca
    66001 atgaaaaaag ttgctgggac tttacgtcta gacttagcaa gttaccgtga attagaagca
    66061 tttacacaat tcggttctga cttggatgca gcaacacaag caaaactaaa tcgtggacgc
    66121 cgtacagtag aaatcttgaa acaaaaattg catgcaccgc tagctgttga aaaacaagta
    66181 gtgattttgt atgctttgac acatggattc ttagatagta tccctgtaga cagtatttta
    66241 gacttcgaac atgaattatt cgaatatctg gataccaatc attcagatat ctttgaaaca
    66301 atccgtacaa caaaagatct tccagaagaa gaagcgttga acagtgcgat tcaggaatat
    66361 aaagacatgt tcttagctac aaaaggaaat aattcctcta cagaagataa attgaaatcc
    66421 attcaaaatg catagtgagg tgagatgaat gggtgcttca ttaaatgaaa tcaagacacg
    66481 tatcgcttca acgaaaaaaa cgagccaaat cacgcgggca atgcagatgg tgtctgcttc
    66541 taagttgaca aaatctgaag cttcttcaca gaagtttcag gtttatgcaa caaaagtaag
    66601 agaaatcgtt acgcatttga ctgctactca gttaaatgat attgcctcag aaaatcctag
    66661 aggggatatc aattacaaca gcatgttgct cagccgtcct gttaagaaaa cgggttatat
    66721 cgtcatcact gcagatggcg gtttagtcgg tggatataac agctcgatat tgaaacaaac
    66781 gatgtctatt ttagaagaag accaccaatc acccgatgat tacgtgatga tcgcaattgg
    66841 cggaactggt gcagacttct ttaaagcacg cggtatcaat ctagcatatg agctgcgcaa
    66901 tctatctgat cagccaagct ttgatgaagt ccgcaaaatc gtaaatatgg caacaactat
    66961 gtaccaaaac gaagtattcg atgaattata cgtatgttat aaccatcata tcaattcgct
    67021 aactagtcag tttcgtgtag aaaaaatgct gccaatctca gacttagatc cagaagaagc
    67081 aactactttc gaacaagaat atatttttga accttcaaaa gaagaaatct tgtctcagtt
    67141 attgcctcag tatgctgaaa gtttgatcta cggtgcgatc gttgacgcca aaacagcaga
    67201 acatgctgct ggtatgacag caatgaaaac agcaactgat aatgctgcta atatcattga
    67261 tgagctgacg gtttcttaca accgtgcaag acaaggggct attacccaag aaattaccga
    67321 aattgttgcc ggagcttcgg cactagaata gtattgagat tggaggaaaa aagatgagtt
    67381 caggcaagat tgttcaagta atcggtcccg ttgtcgacgt ggaattttct ttagaccaat
    67441 ccttaccaga tatcaacaac gcattagttg tttataaaaa tgacgaaaat aaatcaaaag
    67501 ttgttcttga agcagcgtta gaattagggg acggagtgat ccggaccatt gcgatggaat
    67561 caacagatgg tttacaaaga ggaatggaag tcattgatac aggcaaagca atctctgttc
    67621 ctgtaggtaa agaaacatta ggtcgcgtat tcaacgtact aggagatacg atcgatttag
    67681 aaacaccttt cccagaagat gcggaaagaa gcgaaattca taaaaaagca ccagcctttg
    67741 acgaattaag tacaagtaca gaaattttgg aaacagggat caaagttatc gatttgcttg
    67801 ccccatattt aaaaggtggg aaagtcggac tattcggtgg tgccggtgtt ggtaaaaccg
    67861 tactgatcca agaactgatc cataatatcg cccaagaaca tggtggtatt tctgtattta
    67921 ccggtgtagg tgaacgtact cgtgaaggta atgacttgta ttatgaaatg aaagattcag
    67981 gagttatcga aaaaacagcc atggtgttcg gacaaatgaa cgaaccacca ggtgcacgta
    68041 tgcgtgttgc tttgactggt ttgacgattg cggaatattt ccgtgatgta gaaggtcaag
    68101 atgtactgtt gtttatcgac aacattttcc gtttcactca agctggatct gaagtatcag
    68161 ccttgttagg acggatgcct tctgcggttg gttatcaacc aacattggca acagaaatgg
    68221 gtcaattgca agaacgtatc acatctacga aaaaaggttc gatcacatcg atccaagcaa
    68281 tctatgtgcc agccgatgac tacactgacc cggcgccggc aacagcattc gcccatttgg
    68341 atgcgacaac caacttggaa cgtaaattaa cagaacaagg gatttatcca gcggtagatc
    68401 cattggcttc ttcttcaagt gctttagcac ctgaaatcgt gggagaagaa cattataaag
    68461 tagcaacaga agtccaacac gtattgcaac gatatcgtga attacaagat atcattgcta
    68521 tcttaggtat ggatgaatta tccgatcaag aaaaagtgtt agtttctcgt gcccgcagag
    68581 tccaattctt cttgtcacaa aactttaacg tggctgaaca atttactggt cttccaggtt
    68641 catatgtccc tgtagaagaa acagtgaaag gattccgcga aatcttggaa ggcaaatacg
    68701 atgatcttcc tgaagaagct ttccgaagtg ttggccgaat cgaagacgtc gtagaaaaag
    68761 cgaaaacact aggctactag aaaggggtgc cccatggatc aatatttaac agtaaatgtg
    68821 gtaactcctg ccggtttggt ctatgatcac catgcagcta tcgtcgttgc acgtacgaca
    68881 gctggtgagc ttgggatttt gcctaaacat gcaccaatca tcgttccatt gacgattgat
    68941 gaagttcgtg taaaacgaac agattcagat acccacgtcg attggatcgc tgttaatggc
    69001 ggaatcatgg aagttcgtga taatgttgtt tcaatcgttg ctgatagtgc ggaacgtgaa
    69061 cgtgatatcg atgtatctcg tgcggaacgt gcaaaacaac gcgcagaacg ccaaatcgaa
    69121 gaagcaaaag caaaacaaga tacgaacgaa ttgaaacggg caactgttgc gttgcaccga
    69181 gcaatcaatc gtatcaaagt atctaagcac tcataaaatg agatgcggat gtttatccgt
    69241 gtttaagaac agtaaaaaga ggttatgaca gaagtggtca gcttcaagac ataagaagga
    69301 atttacgaaa attgcttctc aaatttttgt gaaatctcag cttatttttc gaagaagcta
    69361 cttctggaac accgtatatc ggaggttgtg aaatcagcgt tttgtcctga gtattagaga
    69421 aacaaatgga aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcaga
    69481 tgattgaaca ccgtttattt ggaataaggg actgcgacaa gacttttgtc tcagtcc
//
LOCUS       DACGLH010000009        59092 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.010, whole genome shotgun sequence.
ACCESSION   DACGLH010000009 DACGLH010000000
VERSION     DACGLH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.010"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(3..305)
                     /locus_tag="IXT21_000622"
     CDS             complement(3..305)
                     /locus_tag="IXT21_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868052.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIETSLLVNL
                     HYSNKNYFKIPAVKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(401..1195)
                     /locus_tag="IXT21_000623"
     CDS             complement(401..1195)
                     /locus_tag="IXT21_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAQ4868053.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHYFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            1814..4648
                     /locus_tag="IXT21_000624"
     CDS             1814..4648
                     /locus_tag="IXT21_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4868054.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            4839..5330
                     /locus_tag="IXT21_000625"
     CDS             4839..5330
                     /locus_tag="IXT21_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ4868055.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5433..6422
                     /gene="manX"
                     /locus_tag="IXT21_000626"
     CDS             5433..6422
                     /gene="manX"
                     /locus_tag="IXT21_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ4868056.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6455..7258
                     /locus_tag="IXT21_000627"
     CDS             6455..7258
                     /locus_tag="IXT21_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ4868057.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7279..8193
                     /locus_tag="IXT21_000628"
     CDS             7279..8193
                     /locus_tag="IXT21_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ4868058.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8276..8650
                     /locus_tag="IXT21_000629"
     CDS             8276..8650
                     /locus_tag="IXT21_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ4868059.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8789..9034
                     /locus_tag="IXT21_000630"
     CDS             8789..9034
                     /locus_tag="IXT21_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4868060.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            9206..10270
                     /locus_tag="IXT21_000631"
     CDS             9206..10270
                     /locus_tag="IXT21_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ4868061.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            10516..10941
                     /locus_tag="IXT21_000632"
     CDS             10516..10941
                     /locus_tag="IXT21_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4868062.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10934..11383
                     /gene="nusB"
                     /locus_tag="IXT21_000633"
     CDS             10934..11383
                     /gene="nusB"
                     /locus_tag="IXT21_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ4868063.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            11480..12334
                     /locus_tag="IXT21_000634"
     CDS             11480..12334
                     /locus_tag="IXT21_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ4868064.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            12335..13672
                     /locus_tag="IXT21_000635"
     CDS             12335..13672
                     /locus_tag="IXT21_000635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ4868065.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            13677..13901
                     /locus_tag="IXT21_000636"
     CDS             13677..13901
                     /locus_tag="IXT21_000636"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ4868066.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13903..14793
                     /locus_tag="IXT21_000637"
     CDS             13903..14793
                     /locus_tag="IXT21_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ4868067.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14802..15620
                     /locus_tag="IXT21_000638"
     CDS             14802..15620
                     /locus_tag="IXT21_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ4868068.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15727..16176
                     /locus_tag="IXT21_000639"
     CDS             15727..16176
                     /locus_tag="IXT21_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ4868069.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            16194..17876
                     /gene="recN"
                     /locus_tag="IXT21_000640"
     CDS             16194..17876
                     /gene="recN"
                     /locus_tag="IXT21_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ4868070.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17931..18047)
                     /locus_tag="IXT21_000641"
     CDS             complement(17931..18047)
                     /locus_tag="IXT21_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ4868071.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(18120..19076)
                     /locus_tag="IXT21_000642"
     CDS             complement(18120..19076)
                     /locus_tag="IXT21_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ4868072.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            19247..19615
                     /locus_tag="IXT21_000643"
     CDS             19247..19615
                     /locus_tag="IXT21_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ4868073.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19785..20216
                     /gene="mraZ"
                     /locus_tag="IXT21_000644"
     CDS             19785..20216
                     /gene="mraZ"
                     /locus_tag="IXT21_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ4868074.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            20237..21196
                     /gene="rsmH"
                     /locus_tag="IXT21_000645"
     CDS             20237..21196
                     /gene="rsmH"
                     /locus_tag="IXT21_000645"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ4868075.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            21201..21602
                     /gene="ftsL"
                     /locus_tag="IXT21_000646"
     CDS             21201..21602
                     /gene="ftsL"
                     /locus_tag="IXT21_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ4868076.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            21603..23795
                     /locus_tag="IXT21_000647"
     CDS             21603..23795
                     /locus_tag="IXT21_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ4868077.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23821..24783
                     /locus_tag="IXT21_000648"
     CDS             23821..24783
                     /locus_tag="IXT21_000648"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ4868078.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24797..26158
                     /locus_tag="IXT21_000649"
     CDS             24797..26158
                     /locus_tag="IXT21_000649"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ4868079.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            26184..27272
                     /gene="murG"
                     /locus_tag="IXT21_000650"
     CDS             26184..27272
                     /gene="murG"
                     /locus_tag="IXT21_000650"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ4868080.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            27287..28507
                     /locus_tag="IXT21_000651"
     CDS             27287..28507
                     /locus_tag="IXT21_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ4868081.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            28663..29988
                     /gene="ftsA"
                     /locus_tag="IXT21_000652"
     CDS             28663..29988
                     /gene="ftsA"
                     /locus_tag="IXT21_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ4868082.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            30011..31252
                     /gene="ftsZ"
                     /locus_tag="IXT21_000653"
     CDS             30011..31252
                     /gene="ftsZ"
                     /locus_tag="IXT21_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ4868083.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            31269..31946
                     /locus_tag="IXT21_000654"
     CDS             31269..31946
                     /locus_tag="IXT21_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ4868084.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31960..32559
                     /locus_tag="IXT21_000655"
     CDS             31960..32559
                     /locus_tag="IXT21_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ4868085.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            32574..32861
                     /locus_tag="IXT21_000656"
     CDS             32574..32861
                     /locus_tag="IXT21_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ4868086.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32910..33692
                     /locus_tag="IXT21_000657"
     CDS             32910..33692
                     /locus_tag="IXT21_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ4868087.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            33713..34420
                     /locus_tag="IXT21_000658"
     CDS             33713..34420
                     /locus_tag="IXT21_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ4868088.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            34682..37480
                     /gene="ileS"
                     /locus_tag="IXT21_000659"
     CDS             34682..37480
                     /gene="ileS"
                     /locus_tag="IXT21_000659"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ4868089.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(37529..39055)
                     /locus_tag="IXT21_000660"
     CDS             complement(37529..39055)
                     /locus_tag="IXT21_000660"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ4868090.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(39070..39717)
                     /locus_tag="IXT21_000661"
     CDS             complement(39070..39717)
                     /locus_tag="IXT21_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ4868091.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39901..40230)
                     /locus_tag="IXT21_000662"
     CDS             complement(39901..40230)
                     /locus_tag="IXT21_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ4868092.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            40407..41135
                     /gene="sfsA"
                     /locus_tag="IXT21_000663"
     CDS             40407..41135
                     /gene="sfsA"
                     /locus_tag="IXT21_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ4868093.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            41151..42164
                     /locus_tag="IXT21_000664"
     CDS             41151..42164
                     /locus_tag="IXT21_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4868094.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            42164..43441
                     /locus_tag="IXT21_000665"
     CDS             42164..43441
                     /locus_tag="IXT21_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4868095.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            43504..46206
                     /locus_tag="IXT21_000666"
     CDS             43504..46206
                     /locus_tag="IXT21_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4868096.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            46358..46675
                     /locus_tag="IXT21_000667"
     CDS             46358..46675
                     /locus_tag="IXT21_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4868097.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            46705..47025
                     /locus_tag="IXT21_000668"
     CDS             46705..47025
                     /locus_tag="IXT21_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4868098.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            47133..48593
                     /locus_tag="IXT21_000669"
     CDS             47133..48593
                     /locus_tag="IXT21_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4868099.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            48661..48882
                     /locus_tag="IXT21_000670"
     CDS             48661..48882
                     /locus_tag="IXT21_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868100.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48913..49095
                     /locus_tag="IXT21_000671"
     CDS             48913..49095
                     /locus_tag="IXT21_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ4868101.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            49095..49508
                     /locus_tag="IXT21_000672"
     CDS             49095..49508
                     /locus_tag="IXT21_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ4868102.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            49631..50812
                     /locus_tag="IXT21_000673"
     CDS             49631..50812
                     /locus_tag="IXT21_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4868103.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            51201..51274
                     /locus_tag="IXT21_000674"
     tRNA            51201..51274
                     /locus_tag="IXT21_000674"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51235..51237,aa:Arg,seq:cct)
     gene            complement(51343..52482)
                     /locus_tag="IXT21_000675"
     CDS             complement(51343..52482)
                     /locus_tag="IXT21_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4868104.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYIKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(52781..53416)
                     /locus_tag="IXT21_000676"
     CDS             complement(52781..53416)
                     /locus_tag="IXT21_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868105.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(53529..54164)
                     /locus_tag="IXT21_000677"
     CDS             complement(53529..54164)
                     /locus_tag="IXT21_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ4868106.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(54198..54659)
                     /locus_tag="IXT21_000678"
     CDS             complement(54198..54659)
                     /locus_tag="IXT21_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ4868107.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(54789..55220)
                     /locus_tag="IXT21_000679"
     CDS             complement(54789..55220)
                     /locus_tag="IXT21_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ4868108.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(55238..55558)
                     /locus_tag="IXT21_000680"
     CDS             complement(55238..55558)
                     /locus_tag="IXT21_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4868109.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            55857..56633
                     /locus_tag="IXT21_000681"
     CDS             55857..56633
                     /locus_tag="IXT21_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ4868110.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            56648..56851
                     /locus_tag="IXT21_000682"
     CDS             56648..56851
                     /locus_tag="IXT21_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4868111.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            56867..57205
                     /locus_tag="IXT21_000683"
     CDS             56867..57205
                     /locus_tag="IXT21_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ4868112.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            57192..57371
                     /locus_tag="IXT21_000684"
     CDS             57192..57371
                     /locus_tag="IXT21_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868113.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(57414..57884)
                     /locus_tag="IXT21_000685"
     CDS             complement(57414..57884)
                     /locus_tag="IXT21_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868114.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            57971..58669
                     /locus_tag="IXT21_000686"
     CDS             57971..58669
                     /locus_tag="IXT21_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ4868115.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            58847..>59092
                     /locus_tag="IXT21_000687"
     CDS             58847..>59092
                     /locus_tag="IXT21_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868116.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDA"
BASE COUNT        19643 a         9903 c        12255 g        17291 t
ORIGIN      
        1 atttaatctt tgtatctttc tggatcaacg aaagtatact ttatatagtc ataacgccga
       61 tgatcgctac gtgcgtccgg cacgtcagtc acgatatcaa acaaaaagta tacacccttc
      121 ttcattctcg ttttcacagc aggaatttta aagtagttct tattagaata atggagattg
      181 actagcaggc tagtttcaat tgctaaaaag aaaacttctg aatcccacac cttataaaaa
      241 tctttgacaa atctattcga aggatcgaat ttaaaccata attgtgtttt tccttccatt
      301 aacatataaa gttcacctca aaaagagtat acgaacaaat gtttgtattg taaatagaaa
      361 aagatgagct aaattaaatg cccatcttct ttgatagata ttataaatct aataattctt
      421 gtttcttttt attaaattct tcttctgtaa tgattccatc atccaatagt tctttatatt
      481 ttcttatttc tgaagctgag gaaattgttg atgtattgga atttgtagca gaaatatttc
      541 ttctttttaa attataaata atttcgttta aagaatcact tatcctatca acagtatctt
      601 tatcaactgc aacattcaat actttaccaa gagtatcaat agttaatgta cccaacagca
      661 ctcctctttt tttactaata ttatttaaat tgtctaatat aatactttgt acattattgc
      721 ctatcatttt ttgttgagct acaattaaac gtttattagt taatgcgtat gcataattat
      781 tgtcatgctt agttgatgag atataattat gcaatcctac aaagcagaag attacgtatt
      841 cgtcactgtt aagttgatct gatattaagt taaaataatg agttgtccat tttttagtca
      901 ttcctttacc gtagccgtat ttaagacaaa attctaccat ctcttcagtg gttctcattc
      961 tatcattacc tatatcatga ttgttttgtg cagtatgatc agatatgttt aattttcttt
     1021 tttctgatgg atccttatct aaatcttcta aattcaaaga tcttattatt ttataaggag
     1081 tagtcatacc gtaaccagct tttttcatac aattgccgca aacaatttgt ttatctttga
     1141 ttttatatga agttaagcca attttacctc cgcactttgc acattgttta ctcacattag
     1201 ttcctcctag atatcagctt tctactacta ttaacattct atcaatagtg accatacttt
     1261 gaccatactt tttagaaaga atagaaaatt ccaagatatt taataataga agaaaagcac
     1321 taacatcatg ggttcagaag aattgagagc ttatcaggaa acagtataat tgaagtgtct
     1381 gtttatccag tagctcaaga agcttaattg aatcttatga ttaaagaggt tgtgacaaga
     1441 cttttgtcac agcctttttt gtttataaaa ataaaagttg tagtggaggc tacgaagttt
     1501 actttgtctt tttacactta atattttttt gccaatttgg aatcaggatg ttttgataac
     1561 tatcgttttc aattgatgga agctatattg tttgctatca gtgaaataat tatatggtaa
     1621 acagtgttct ttttcttgtt tccattataa aaatcgtatc gggatttttc gttcttttaa
     1681 attgaacatt tctatctatc cgcgtgttat caacaaaaca aaatcaggta aatagttgac
     1741 gaaacagaaa taaatacata gtattatgtt ttgaagccta agtacaatag tctatagaaa
     1801 aggagcgata ccattggcaa aacgcattga ccgaatttat atgtatgtca aagaaaaaac
     1861 cgctcattta acaaaggcag aatatgatca aggagtaaca acacaggaaa ttgctgaagt
     1921 gttagggatt cagcgaacaa atagcagtaa agatctgaat cagctagtgc gcgaaggaaa
     1981 actgataaag acagacggac gaccagtacg ttacatatat caaacactcg ttacgcacgt
     2041 aaagccagtt acaaaacatg tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa
     2101 tacactccaa atagatacta aggatatctt tgcaaaaatc attggagcta acggcagcat
     2161 gaaaaattca gtggaacagg caaaagctgc gattttgtat ccgccaagag gattaaattg
     2221 tttgattaca ggacctaccg gatcaggaaa gacttatttt gcacacgcga tgtatcattt
     2281 tgcaaaagcg catcatgtaa tagatgagga aacggaactg attgttttca attgtgctga
     2341 ttatgccaat aaccctgagc ttttgatgag tcatttattt gggtacgcaa aaggtgcttt
     2401 tacaggcgca gatgaagaaa aaatggggat tatcgatcaa gcggatggcg gtatgctgtt
     2461 tttggatgag attcaccggt tgccgcctga aggtcaagaa atgatctttt actttatgga
     2521 tcatggaact tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa atgtacggat
     2581 cgtaggtgcg accacagaag acccaggttc ttctctatta gaaacttttg ttcggcgtat
     2641 cccaatcaat atcaaacttc cggcttttga aaaacgtcct gcaaatgaga aagtcgattt
     2701 agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga
     2761 agatgtagca aaagcattga tcggcagtgt gacctatgga aatataggac aattgaagtc
     2821 aaatatccag ttagtatgtg cacgaggatt tctaaatcat atgcaatcac ccgaaatctc
     2881 gatcacaatc gatgatttga cagaagggat tcgtagcgga ttgattcaat tagcaagcaa
     2941 ccgcagtgca atggcagagc tttctaaaat attggagcca aagataactg tagcaccaaa
     3001 tgatacgatc atgaaaatcc aatcggactc ctatgagtta ccttataatc tatatgatat
     3061 catcggagat aaagcagctt tactgaagtc ggatggtttg gatcaagaag caatcaatca
     3121 tttcatttct acagatatca acatccatct gaagtctttc tataaagacc atggtttttc
     3181 ttttaatgca gacagcaaat tagcagaatt tgtagatccg aaaatcatag aagtgaccaa
     3241 tcagatttat gtaatggtca aaagatcttt accatatgag tttcagcaaa actttattta
     3301 tgctatgagc ttgcatatca gttctttttt gaaaaaaatc aacttaggag aagaacggca
     3361 tacgaatgat aacatccgtg agatggcttt tgattttcca gatgaatatg ctgttgcgaa
     3421 agaagtgcgt agttatatcg aaacgtattt tcaagtggag attccagaaa gtgaagacta
     3481 ttacttgacc gttctactcg tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt
     3541 agtcgttgca gctcacggaa acagcacagc aagcagtatg gtccaagttg tccaacagtt
     3601 gttagatgca gagaacgtac gagcagtaga catgcctttg gatatggatc cgaaaacagc
     3661 attggaccga attgaacgca atgtacaaga agtagatgaa ggaagcgggg tcatactctt
     3721 agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc
     3781 agtacgtact gttgatatgg taacaacttc tctagttttg gaaactgtac gaaaggtctc
     3841 agtattaggg acagatctag atatgttgca tgaatcattg aaaaattttc gaggttacgc
     3901 agaaatatct tctgtagaat ctgttcagga agcaaaacct attcataaga aaaaagctgt
     3961 tttggctatc tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg
     4021 agcggtaaac aagcgtcaag aaacagaatt agaagttcta gctttgtcga ttgtggaagt
     4081 gaaagaagaa ctgccagtta tccaaaaaga atatcagatc atagctacta ctgggattac
     4141 tgatttgaat atccaagccc catttattcc tttagaacgt tttattgatc aaaatatcga
     4201 agtcattttg gatcagttgc tgatggaatc agaattggag gaaactgaat tcatctccct
     4261 agatgaagag tcagctaaaa atacgtgtgt tgagtttatc tctgataact tcatctttat
     4321 taatggtagt aagttaatcg atccaatgtg gcaattttct acacaaatct ctcagacgac
     4381 gggaatcggc gatgaagaat atggattcaa aatcaaccta gtgatgcaca cagcaggaat
     4441 gatcgaacgt attattcgga atgaaccatt gactgtggaa gaaaacgaat tgacaaatac
     4501 aacgaatgat cctctttata gccaattggc agcctcagtt gttctgttgg aagatcagat
     4561 aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta cgtctagtcc ataatcagtt
     4621 agataaaaaa gaatacactg tgccatgaat acacaaaaac gatacacttt tatagtgtat
     4681 cgtttttgtg tattggttaa gaatccttta aattcaatta gtgtatcgta aaaaagttgg
     4741 cacgcttttt gcttatagta gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg
     4801 ggtttctgtg aagtagtgag gaagaaggag ttttgcagat ggatattcga ttggcacgta
     4861 tagatgatcg tttaatccac gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg
     4921 aacgtatcat tgtgatcagc gatgtagtcg ctcaagataa attgagaaag tttctactaa
     4981 aagaagctgc accgccgggg atcacagcaa atgtaataac cgtgcagaaa atgattcaaa
     5041 tctatcatag tggattatta tcacgagcaa aagtgatgct tttattcacc aatccccaag
     5101 atgttgaagc ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc ggcggtatga
     5161 gttttactga aggcaaatca atgattacca attttgtttc agtaaatcaa caagatattg
     5221 attcctttcg ttacttgaac agtcaagaag tggagttaga gattcgaaaa gtacctgctg
     5281 acaggaaaat ccttttgatg gaactactag cgaaaatcaa aaaggtgtag caagaatggt
     5341 tgttggaaaa ggtcaaataa ttctgttgta atggttagca attattaata tttttgaatt
     5401 tgttacaaca aaagtaaata ggaggtataa atatggtagg gattatccta gcaagtcatg
     5461 gacaatttgc tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa caagaaaacg
     5521 tcaaagccgt gattttgaaa ccaagtgaag gtccagatga cttgagagcc aaattggaag
     5581 aagcggttgc ttcttttgat aatcaagacg aagtgttatt cttagttgat ttatggggcg
     5641 gaacaccgtt caatcaatca aacacattat tcgaagagca caaagataaa tgggcaatcg
     5701 tcagcggttt gaatttacca atgttgattg aagcctacgc atcacgcttt tcaatggaat
     5761 ctgctcatga aattgcagca cacatcattg aaacagcaaa agacggtgta aaagtaaaac
     5821 cggaagaatt agaaccggca gaagcaccga aagcagcagt tgaagacgca caacctaaag
     5881 gagcacttcc agaaggaact gtggtcggag acggaaaaat caaatatgta ttagcacgtg
     5941 ttgactcacg tctgttacat ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa
     6001 accgtattat cgttgtttca gatgcagtat caaaggatga tctgcgtaag agactaatcg
     6061 aacaagctgc tcctccaggt gtaaaagcaa atgttattcc tattagcaaa atgatcgaag
     6121 tagcaaaaga ccctcgtttt ggaaatacca aagcattgct tttatttgaa aatcctgaag
     6181 acgtattgac agctgttgaa ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg
     6241 cgcattcggt cggtaaagtc gttgtaagta aagtattgtc tatgggacaa gaagacgttg
     6301 aagcgtttga gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg
     6361 attcacgcga taacatggat gatattttga aaaaagcaaa agctgaatta gcaaaagcgt
     6421 aataaataat attaaaattt ataggaggct tatcatgtct attatttcaa taattttagt
     6481 cgtattagtt gcctttctag ctggtatgga aggtattctt gatgaatttc aatttcatca
     6541 accactagtg gcatgtacat tgatcggttt agttaccggt aacctcgaag ctggtatcgt
     6601 acttggcggt acgcttcaga tgatcgcttt aggttgggca aatatcggtg ccgctgtagc
     6661 accagatgct gcattagcat cagtagcatc agcaattatt ttagttttag gtggacaagg
     6721 tgttaaaggt gttccttctg cgatcgcgat tgccgttcct ttggctgttg cagggttgtt
     6781 cttaacaatg atcgtacgta caatcgctgt tccaatcgtt catttgatgg atgcggctgc
     6841 tgaagaagga aacatccgta aggttgaaat gtggcacatc attgctgtat gtttacaagg
     6901 ggttcgtatc gcggtcccag cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc
     6961 attccttgaa tcaatgccag catggttaac agatggtatg gctatcggtg ggggaatggt
     7021 cgtagctgta ggttacgctt tggtaatcaa catgatggct acaaaagaag tatggccatt
     7081 cttcgttatt ggtttcgttg tagctgcaat ttctcaatta acattgattg ctctaggtgc
     7141 tttaggtgtt gcattagctc ttatttactt gaacctttct aaaatgggtg gttcatcaaa
     7201 taatggaggc ggaagcaact ctggtgaccc tttaggcgac attttgaatg actattaatc
     7261 ttgaaggagg aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta
     7321 gctgttgcgt ggcgctctac cttcattcaa ggttcatgga actacgaacg tatgcaaaac
     7381 ggtggctggg cattttctat gattccagca atcaaaaaat tatacaaaac aaaagaagaa
     7441 cgttctgcag cattaaaacg tcacttggaa ttctttaata cacacccata cattgcttct
     7501 ccaattttag gggttacatt ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat
     7561 gatgtagcga ttcaaggggt taaagtaggt atgatgggtc ctttagctgg tgttggtgac
     7621 ccagtgttct ggtttacagt acgtccaatg ctaggtgcac taggtgcttc attagctatg
     7681 ggaggaaata tccttggacc aatcatcttc ttcttaggtt ggaacattat tcgttgggca
     7741 ttcatgtggt atacacaaga atttggctac aaagctggtt ctaaaattac tgatgacctt
     7801 tctggcggat tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta
     7861 gctgcccttg tacaaagatg ggtatcaatc aaattcttac cagttgtatc aactgtaaaa
     7921 ttagataaag gtgcttacat cgaatgggat aaattgcctt caggtggtga aggaatcaag
     7981 agtgcatttg aacaagttaa taatggctta gccctttctc ctgaaaaggt aacaacatta
     8041 caagacaact tagaccaatt gattcctggt ttagctgcat taggattaac attgttatgt
     8101 atgtggttgc ttaagaaaaa ggtttctcca atcgttatca tccttggttt attcgtagtc
     8161 ggtgttgttg gacacgtcat cggactttta taatccataa aaatatagct cgggcgttgt
     8221 cccgagcttt ttttatatag tatactgttc ataaacaaat atctggaggg agaaaatggt
     8281 tcaatcaatc aatacaaaag tagacttggt aattgatgct acttcgttta cgggtctgac
     8341 agattacggg caaatcatga taggtgataa tggttttgag ttttaccatg cgcgagatag
     8401 acgcaaatat atccaaattc catgggaaga agtggattat gtactagcat cagtcatgct
     8461 aaagggaaaa tggattcctc gatatgccat tcaaacgaaa aaaaatggta catacacatt
     8521 ttcttccaaa gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag atgaagatag
     8581 aatggtacaa tcattgagtt tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa
     8641 acgcaagtaa aaaataaaga aaacttctaa gaggaggttt tctttatttt tttactttgc
     8701 agaagaaaat atgaaatagc aaaaccaatt atttgtcccc tttaaatccc atatgctaaa
     8761 attgattagc aaagaaggag gttatattat gagttggtta tggacattga ttgttggagc
     8821 cgtgatcgga gcaattgcag gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac
     8881 gaatatcatt gctggactag tcggttcaag tattggacaa gcgattttcg gcgaatgggg
     8941 accaaaagct gcaggtatgg cacttattcc atctatttta ggcgcagtga ttttggtagc
     9001 agttgtctca tttttctttg gaaaacgatc ataagttttt acaaaatagt aaactaaaag
     9061 gagttgcagc gagactgtga ctcctttttg gctttattta tttaaaataa aaagaagttt
     9121 tccggaaatt tttatttaac tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc
     9181 gtgcaaagaa agagggagtt tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat
     9241 gcaagaagaa aatttggatt catttttggt taccagtccg tacaatcttc gttacttaac
     9301 gaactttaca ggtacgacag gattagctgt tattactttg gaaaaagcat tttttattac
     9361 tgattttcgt tataccgagc aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa
     9421 tgtcggacct atttttgatg aggtagcaaa cttagtacgc aaagaagaac tgcactcatt
     9481 agcttttgag gaaacaacag tctcattttt agaatattcg gttttagaag aaatcatcga
     9541 tgcggaatta gtacctgttt cacagatgat cgaagaactc agagaagtga aagatgaaga
     9601 agaaatcgct attatcgaaa aagcatgtca tattgcggat atggcatatg atcacatcct
     9661 gaaaatgatc caaccgggca tgactgagat tgaagtcgca aatcaactag acttttatat
     9721 gcgttctctt ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg gattgcgttc
     9781 ggctatgccg catggtgtag caagcaaaaa aatcatcgaa caaggtgact tgatcacatt
     9841 ggactttggt tgctattacg aagggtacgt ttccgatatg acacgtacat tcgctattgg
     9901 tgatcctggg gaaaaattaa aagaaattta tcaaatagtc ttagatgctc aattagcagt
     9961 tattgatgct gcaaaaccag gaatgactgg tgtccaatta gatgcagttg ctagagatta
    10021 tatttcaaaa catggttacg gggaagcatt tggacattct acaggacatg ggatcggttt
    10081 agagatccac gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa
    10141 tgtgatcact gatgaaccag ggatctactt accaggaatc ggtggagtac gtattgagga
    10201 tgacttgttg atcacagaag atggaaatcg tgtgttgact cgttcaccaa aagaattgat
    10261 catcttatag tttcatcaat agaagaagta agcgtaaaaa tcagtgttca cagactttgt
    10321 tatagaaaat cactcgattt aatgatacac tatagaagaa cttttaagct gtaagagata
    10381 ataaagttgg gtgttttctc caattcatag cagtttgcag ctgatcggcg gcagttttct
    10441 taccacgtga tcactatgcc gcatcataga gaattcggat gtatctcgtg cttaggcaaa
    10501 gggaggaata aataaatggc agatgaaaaa aatttgattt taaacgcaaa ccaagattta
    10561 ggagagatcg tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc agcttctaaa
    10621 gtagaaggcg tatacggaat gcgcggaact tttgccagca atgtcactga attattagga
    10681 cgtgcagctc acggaaaagg tgtttactta gcaaacgagg aagatggctt gaaagtagat
    10741 ctttactgtt atctagaata tggtgtttca gttcctaaag tagcaatgga aatgcaggaa
    10801 cgggtcagcc agcaagtcct tttcatgacg gatatcgatt tatctgaagt caatatccat
    10861 gtagtagctg ttgttcctga aaaattagaa caaccaacaa tcgaagaact ttttgaagac
    10921 gaagaggaag agaatgaata aagaattatc aagacacgag attcgggaaa tggcattgca
    10981 ggctttattt ccattagact tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc
    11041 aatcgaatta gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg ttcctgttta
    11101 tcttgatacg ctagttggcg gtgtctgtgc aaaaaaagat gaattggata aagtgattga
    11161 aaaacattta aaaaacaatt ggcacatcaa tcggatctca aaaatggatt tggtgatctt
    11221 gcgtatcgcg atttttgaaa tgacttatgt gacagatgta ccagcggctg ttgctttaaa
    11281 tgaagcaatc gagttggcaa aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg
    11341 tgttttatcc aatgtattga aagaattaga agctggggcg taagtcctag cttttttcta
    11401 tgaatcatca taaacctgtt ataaatcggg aaaattctat ggtagaatga aaacacaaaa
    11461 aatatctggg agtggttaga tggcagaatt gattaacgga aaagaattag ctgaaaaaat
    11521 gcaggcagag attgctgaaa aaatccaaaa attgaaagat aatggtatcc atcccggatt
    11581 agtcgtttta ttggtagggg aaaatccagc cagccaaatc tatgtaagaa ataaagaacg
    11641 tgtagcaaaa gaaatcggaa tccattcatt ggtagagcgt tatcctgaga cgatttctga
    11701 agacgaacta cttgcagaaa ttgaaaaata caatcaagat cctagttttc atggtatatt
    11761 ggtccaatta ccattgccaa agcatataga tgaagaaaag atcctgttag ctatccgccc
    11821 tgaaaaagac gtagacggtt ttcatccgat gaatttaggc agactccttg cgggtaaacc
    11881 ggacaagatt ccttgtactc catacggcat catgaaaatg tttgaagctt attctatcga
    11941 tccggcgggt aaacgagcag tagttatcgg acgaagcaat atcgtcggta aaccgatggc
    12001 tcagctactc ttgatggcag atgcaactgt tactgttgct cattctaaaa ccgaaaatct
    12061 ggctgaactg gcacaagaag cagatatttt agtcgtagca ataggacgtg gacattttgt
    12121 cacaaaagaa ttcgtaaaac caggtgctgt agtgatagat gtagggatga atcgtgatgc
    12181 taacggtaag ctgatcggtg atgtgaaatt tgatgaagtg gagcctattg caggctggat
    12241 cactcctgtg ccaaagggtg tagggccaat gactatcacg atgctgatgt atcaaaccgt
    12301 aaaatctgcc gaagcttttg gagcaggtga atagatggca caagaatatt taactgtcac
    12361 tacattaacg aagtatttga aaagaaaatt tgatgccgat ccttatttag aacgcgtata
    12421 tttaacaggc gaaatatcga atttccgttt acgtgccaat gcccatcaat atttcagctt
    12481 aaaagatgac catgcaaaaa tatctgctat tatgttcaaa ggagcattcc aaaaattacg
    12541 attccagcca aaagaaggaa tgaaagtcct tgtcgttggg cgtatttcat tatatgaagc
    12601 gggaggttct tatcaaatct atgtagagca catggagcct gatggaatag gtgctttata
    12661 tcaagaattg gaagaacgaa aagaaaagct gatgaaggaa ggcctttttc aagggcctaa
    12721 aaaaatgctg ccacgtttcc caaagcgaat cgcggtcctt accagtccaa gcggagcggt
    12781 tatccgcgat atcatcacga cggtgaaaag aagatatcca attgtccaat tggttttgtt
    12841 cccaacagtt gtacaaggaa accaagcagc ggacaatgtt gtcaggaata tccagagagt
    12901 agaagagatg ggcaatttcg atacaatgat catcggtcgt gggggtggtt cgattgagga
    12961 tttatggccg tttaatgaag agcgggttgc tcgggcgatt tatcaagcta atacgccggt
    13021 gatttcatct gtaggacatg aaacagatac cacgatcgct gatttagtag cagatgttcg
    13081 ggctgccact ccaacagcag ctgcagaatt agctgttcct gttttgtcag aagagttgat
    13141 gcgcattcaa gaacggcaag ctagattgca gcaggcgttt gtacgccaga tccagcgcaa
    13201 acaagaacgg ttcgagcgtg ctcgacgttc ctatgtattt cggcagcctg aaagattgta
    13261 tgaaggtcag accatcaaat tagaccagtt gaaacagcga ttatatcaag ctacacagca
    13321 aatttatcat gcgaaagaaa aacaaacgat cgcattaagc caccaattgg aacaagtggc
    13381 tccgctttat cgagttaaag cagctcgcca agaaactgct tatctagaaa aacgcttaga
    13441 agagaaaatc caacaatata tgaaacaaca gcagcagcgt tttgctcaag ctgttcaatc
    13501 tcttgatttg ttgagtccat tgaaaatcat gggaagaggc tacagctata cgacgttaaa
    13561 tgatcaagta gtaaaaagtg tcaccgagat tcatcaaggg gatttgctga cgatccatta
    13621 tcaagatgga atcgttaaag gacaagtaca agagatagac aaaggagaat aaaaatatgg
    13681 aaaatccaac ttttgaagag tcactgcaag aattagaaaa aattgtcatg cagttagaac
    13741 aaggagacgt tccattagaa tcagctctag attcgtttaa acgaggaatg gaattaagca
    13801 aacattgcca agatacatta gctaaagcag aagagacctt gacgaaaatg atgacagaat
    13861 caaatgaaga agttgatttt gacggaaacg aggaaagctg agatgagatt aactgacttt
    13921 tcagctattc atctacctgc tgtagaaaaa gaaatcttgt cttttttaga tgagcataca
    13981 acggatagag gactctatga tgcgatgagt tattcagtga aagcaggcgg gaaaagaata
    14041 cgtcccttat tacttttaac ggccgttgct tcctttgatg agcctattga tgttcccgtc
    14101 tatcaagtag cagctgcttt agaaatggtg catacgtatt ccttgatcca cgatgatttg
    14161 cctgcgatgg acaatgatga tcttcgtaga ggaaaaccta ccaatcataa agtattcggc
    14221 gaagcattag ctatattggc cggagatggt ttgcttacag gagcattcca gctaatcagt
    14281 atggcacact taggtaattc tcctaagctt ctgctgcttc agcaattagc tgtttgtgcg
    14341 ggatcacaag gtatggttgc tgggcaagct gcagatatcg agggcgaatc gaaaaagctt
    14401 tctttagaag aattagcatt tattcatgaa agaaagacag ggcatttgat tcgttatgcc
    14461 ttgctggctg gtggcatatt agcgaagcaa cctgaagaaa tcttattgct acttcaacga
    14521 ttagccgaac atttaggttt agcttttcag ataagagatg atctactcga tgtgattggt
    14581 acaacgaaaa cattagggaa aacagctggt aaagatgaac gtatggaaaa aaatacttac
    14641 ccgcgtttat taggcctaga aaagacacgt gaggcgttgg agattgagct gttatccgcc
    14701 aacaagatca tagacaaatt agaagaggac gttttttctt ttgatggcgg gctgatgcgc
    14761 cagatgatca agcaatttga tgtggagaaa tgagggaaag aatggaaaaa gaacgcgtag
    14821 atgtattagc ggtcaaacaa ggattatttg aaacaagaga acaagccaaa cgttcagtga
    14881 tggctggttt gatttacaac gaaaaaaacg agcggtttga taaaccggga gaaaaaattc
    14941 cagtgagtag tgaattaaga gtcaaaggta aaaaacttcc ttatgtttct cgagggggat
    15001 taaaattaga aaaagcactc aaagcatttg atctatctgt tgaaggaaaa acattgttag
    15061 atattggtgc atccactggc ggatttactg atgcagcttt gcaaaatggc gcaaaaatga
    15121 gttatgcttt agacgttggc tacaatcagc tagcttggaa aataagacaa gatcctagag
    15181 tcgtcgtaat ggaaagagtg aattttcgtt atgcaaaacc cgaagacttt accttaggaa
    15241 ttccagaagt tgctgttatc gatgtttctt ttatttcact aaaactgatg ctgcctccat
    15301 tacatgctat tttaaaagaa gagggagaag tgattgcttt aatcaaaccc caattcgaag
    15361 caggcagaga agctgtaggg aaaaacggaa ttgttcgtga ccctcagacc catcaaaaag
    15421 ttttagaaga cattttgtca tttgcggcac aaggtggata tgatgtgctg gaattaagtt
    15481 attctccgat cacgggggga gaaggaaata tcgagttctt ggctcacttg aggaaagtac
    15541 ccgaaagcgg aacaatcaat tcagctatta atatggcaga agtggtttca aatgcacacg
    15601 aacagtttga ccataaataa attgtaactg tgaaaatcaa gtgagaagta tgcggattct
    15661 tctttattat accaaggaaa taagctatca ttgattaaga agaatatgct aaggagcgaa
    15721 gacgtaatga gaaaaaaaga tcggcatcgt ttgattactc gattgttgag tgagcaagat
    15781 atccgaaagc aggaagaatt tgttgaaatc ctgaaaagca aaggaatatc tgttactcaa
    15841 gctacgattt ccagagatat aaaggaactg aaactgatca aggtaccggc ttcagatggc
    15901 ggctaccgtt atagcatacc agctgaaaca aacgaagatg taaatactaa attggaaaaa
    15961 ctattaaaag acgctttcgt ggcagttgac caaatggaaa aatttgttat tttgaaaaca
    16021 ttgccgggaa atgcttcggc agcagcaaat ttgattgata aacgttacaa gaaagaatta
    16081 ttttcgatta tcaatgatga cgataatgtt ttgatgatta cacgaactga agaccaggca
    16141 gttttattga agaaagactt cctgcattac ctttaagttg aggagcgaat gtgatgctac
    16201 aagaaatcag tattactaac tttgcgatta ttccggaatt gcggctttct tttcacgaag
    16261 gaatgaccgc gctgaccgga gagactggtg cgggtaaatc cattattatc gatgctttag
    16321 gtctgttagc aggaggaaga ggttcaagcg attatatacg tcaaggagca gaaaaatgtg
    16381 tgcttgaagg gttgtttgaa ctaccaaaac aagaaggatt ctctgaactg atggttgaat
    16441 tagggatcga aacagatgag gacaatctga ttgttcgacg ggatatgtcc ttaactggaa
    16501 aaaatgtttg tcgagtgaat ggtcatatta tcacattagc taatctgaga agaatcggca
    16561 gttatttagt agatatccaa ggacaaaacg aacatcagga actgctccag cctgaatcac
    16621 atcttgcttt attggatcgt tttggagatg tagctttcca acagaagaaa aagagttatc
    16681 agcaagaata tatctcttat cgtgagctgg aaaaaagggt acgaaaagtg cagcaaaatg
    16741 aaaaatccta tgttcagaga atagatatgc tccatttcca gcaagaagaa atagctgctg
    16801 ctgatttgca agtcggagaa gaagagaaat taaaagaaga aagagaaaaa cttagcaatt
    16861 atcaaaaaat tgctgatgga ttagctgcag gttacggagc actgacgaat aatgaacaaa
    16921 acagtgtaga tggtgttggc ttggccgttt cagaactgca agggatcgcc catctagatg
    16981 tggagtatga agcaatttat gaaaatatcc aaagtgctta ctatttgctg caagatgcaa
    17041 tcggcgatat gagtcgtcag attgatttat tggagttaga tgagagtcgt ttagaagaag
    17101 ttacgcaacg tttagaatta atccgacagt tgaaacgtaa atacggcgaa tccattgagt
    17161 caatattggc ttactatgac gaaatcaccg aagagctagc ttcttctgat ttttcagaag
    17221 gtcaattgga caaaatgaaa gaggaactag agcaaaaaga gcttctactt cagcaacaag
    17281 ccgcagatct tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa tccattctgc
    17341 atgaattgaa aagtctgtat atggaaaaca ctgagtttga ggtacgtttc ttaaaagaag
    17401 aaaatagaca attgaatcat gatggatttg accaaatcga attttacatt acgaccaatc
    17461 caggagagcc actgaagcct ttggtgaaag tcgcttctgg tggcgaactg tctcggatgt
    17521 tgcttgcttt gaaaactatt ttttcttctg agcaaggtgt gacaagtatt atttttgatg
    17581 aagtggatac aggtgtgagc ggaagagttg cacaggccat cgcagataaa atttctcaaa
    17641 tatcgaaata ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct gcagtcgccg
    17701 actatcaata ttatattgtg aaagcagtga ttgatggacg tacgcaaact tctgtttcag
    17761 agttgaaaac aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga agtgagatta
    17821 caaaactcac actcgaacat gcaaaagaac tattgaaact cgcaaaaagg acctgagcca
    17881 cctcgcagtg gttcaggtcc ttttcagcag ctgatagcta tattttattt ttacatgaca
    17941 ctcattacag cagtcaatac aacagatcca atcgttaaaa tcagcattgt ccaaatgaca
    18001 aattttgtta ctttagcaaa agtacttgtt tgtttttttt cacgcattgt tttcaacctc
    18061 gtcattaatt agttgattca agtgtacaat cttttttcca tttccacaag agggcttttt
    18121 tataaaaact tccctttttt taaataatag gtagttacga tacaaataaa taatgttaat
    18181 agaactagcc atataaaggc atcatccctt tcagcaatag gtaacttaat gttcattcca
    18241 tagatactgc cgacgattgt cggtatagtt aaaataatcg ttaaagaagt taaaattttc
    18301 ataatattat tcagattatt cgatacgatt gctgaaaaag tctgattcat ttgttctact
    18361 agttttaatt gaatgcgaga agtagtcatt gcctgctctg tttccaccaa tacatcacgt
    18421 aaatgatttt tcaaagcatg attttcttgg aaaagtttat tttgcgctag ttctttcaat
    18481 gcatctaaat tagcagaagt cgcttctctg aaataaacga ggcttttttg gatatccatg
    18541 atttgataaa gttgtttgtt ttcagtggat acttgaagtt tggcttccaa agtatcgcat
    18601 tgagattgaa gcgtagctaa ataactgttg taagagagtg tgacctgcca aatgaattga
    18661 aagaagatat tgagattagg cgaaatatca ctttttggaa aggtctggct aaagactttc
    18721 tttaaaaaca gtggttcatg attcgtaatt gtgataagct tctttttggg cgtcaaaata
    18781 atagagagcg ggtatgtatc cacttgaaaa taaccgctag gacttgtttt agcaaaagga
    18841 tattgtaaca ataataacgc tggctttgtc aactttgatt gattgaattc ttctgtacgg
    18901 gaattttctg catcgtccaa tatggaagtg atgtaatctg acggaatttc aaattgatgt
    18961 gtcagctgat gaatctcttc ttgattagga cgttctacac aataccagat cgtttggtca
    19021 gtttcctttc cggtattacg cagtttctca ttatctaatg taaaatattt gatcatgatt
    19081 ttcctcctgt tgcctgtttc ttttattata acggataggg aattggaata catggaatgt
    19141 gtttgtaaga tatagaatat aactgcataa atttaaataa aatgaaacag ttgtgtaaag
    19201 tatatacagt atacttgaag caacgataaa aaaggagttt acgttcatgg gcacattcac
    19261 accacttatc ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt gccaatttct
    19321 tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt tggcgattcc
    19381 ttttttgtgg ggcggtatgc atgcattgtc tagaaataca gggacaattt ctatcttacc
    19441 gtttttcttt atttctgttt tgctaatggg aatttgtatt gctgttttcc aagcatatta
    19501 ttatgaagaa attatttatc cgcgttattt caagatgact tggcgtttgg tttttctgtt
    19561 gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa
    19621 atagaaaagc tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg
    19681 tttatgaata aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat
    19741 tgtggtagac taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat
    19801 tccaacataa tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac
    19861 ttggcgaaaa gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga
    19921 gcgaatggga aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac
    19981 gtacttttgt gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac
    20041 gaatcaatat acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca
    20101 tcggtgtatc taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag
    20161 cagcagaaga gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat
    20221 aggaagaggc tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac
    20281 ggtagatgga ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc
    20341 cggacatagt gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga
    20401 ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg
    20461 tatggtcaca tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt
    20521 agataaggta gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc
    20581 agaacgtgga tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc
    20641 tcctttatcc gcttatcacg taattaatga atattcctac catgaattag taaagatttt
    20701 cttccgctat ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg
    20761 aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc
    20821 agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat
    20881 tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt
    20941 gaaagtcaat ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa
    21001 aagtatgttt aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc
    21061 tgacgaattc caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga
    21121 agagctaaca gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag
    21181 aatgaaagag gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt
    21241 tacctgaatt ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa
    21301 tcattcttcc acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg
    21361 ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca
    21421 caaatatcag cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa
    21481 agcaagaaaa aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta
    21541 aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt
    21601 aaatgagtct taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga
    21661 acaatcgcaa aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat
    21721 ttgccgtccg tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg
    21781 aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt
    21841 atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac
    21901 tctcaaaaac atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata
    21961 aaatagcaga aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt
    22021 tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata
    22081 tctcccaaga aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc
    22141 tttattttgt cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta
    22201 ttggatacgc agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt
    22261 ctgcttacaa tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt
    22321 ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata
    22381 tctacacgac tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa
    22441 atgaagaata tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa
    22501 tcttggcaat gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa
    22561 aagatgctat ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga
    22621 aagttttcac tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc
    22681 aatctggaaa aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag
    22741 gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg
    22801 aacagcgatt aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta
    22861 ctcattctgg attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc
    22921 gagcaatgag tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt
    22981 tcacttctat tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg
    23041 atccccaaac tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa
    23101 aagaaacaac agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg
    23161 gtagtgctta tggtgtatat agtgtacctg gatacaatgt atcggcaaaa accggaacag
    23221 cccaaatcgc ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga
    23281 ttgtggagat ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc
    23341 ctaagacata tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa
    23401 tggattttaa agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg
    23461 tagccgatta tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac
    23521 tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg
    23581 atcagttgat ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg
    23641 acgtaacagg atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag
    23701 ttagttttga tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca
    23761 ctgaagacaa acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc
    23821 atggaatgga cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca
    23881 atgccgctat ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag
    23941 ggaccaaagt ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg
    24001 atcggctcta ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact
    24061 cttttcatct tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt
    24121 ataaaaatat tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag
    24181 attctagggg gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg
    24241 aatttcttcg gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg
    24301 cttgttggat tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc
    24361 ttaggaagta tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat
    24421 gtattaatca tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga
    24481 aaacctgcca agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg
    24541 gcaatttcta taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt
    24601 atggaaacag caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt
    24661 tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt
    24721 gatacagttt tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg
    24781 taggagatga cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt
    24841 aggactcgca aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt
    24901 caccgttaat gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt
    24961 agggataaaa gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt
    25021 gatggtcaaa aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat
    25081 gggtattccg gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat
    25141 tgggattaca ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa
    25201 tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg
    25261 tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact
    25321 catgggaatc acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca
    25381 tattgattat catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa
    25441 catgacagaa aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc
    25501 gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt
    25561 gtatgctgac gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact
    25621 tggcgtacca ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt
    25681 gtatggtata tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca
    25741 tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac
    25801 caatatttta gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt
    25861 ggctggcgga ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat
    25921 caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc
    25981 aggcatcgag acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga
    26041 aaacagtaca gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata
    26101 cccaaatttt gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa
    26161 aaagtagaag cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac
    26221 atatttatcc agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat
    26281 tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc
    26341 ctttccatac cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga
    26401 cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac
    26461 cagatatcgt tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca
    26521 aaatgggtat tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat
    26581 ttttaagccg atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc
    26641 ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa
    26701 aatcagatat tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg
    26761 gcggtagtca aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata
    26821 ttccttcttc ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa
    26881 ataaaataaa tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag
    26941 aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat
    27001 tcacagctct ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc
    27061 agactaaaaa tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg
    27121 aactaacagg tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc
    27181 aaaagcaaat gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt
    27241 atgacttagt aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa
    27301 agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa
    27361 ggctaaccga aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga
    27421 cataacatta gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca
    27481 gcaagaagaa agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa
    27541 aaacggtgga ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg
    27601 gcacaaagta ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat
    27661 ttttttgatc tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa
    27721 taagacagtc aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt
    27781 ttggggacag tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag
    27841 aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga
    27901 atataaagag attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg
    27961 aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt
    28021 caaagactct gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt
    28081 acaaaaagca atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca
    28141 attgaatatg aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat
    28201 gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt
    28261 tggtatcttc tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga
    28321 agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga
    28381 aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga
    28441 ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga
    28501 aaattgataa aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg
    28561 tagaattgga agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg
    28621 ttcgcttgta tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg
    28681 tatgtaggcc ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac
    28741 agccaaatga atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt
    28801 atcgttgata tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa
    28861 aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta
    28921 gaaaattgcc agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat
    28981 gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg
    29041 tcaatattgc cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga
    29101 atgatcggcg taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc
    29161 cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact
    29221 cctttagcgc tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc
    29281 attgatatgg gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt
    29341 accaatcttg accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat
    29401 acgtcattta acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg
    29461 acatcaccag atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc
    29521 gatgaacgct acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca
    29581 aaagatgcat tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc
    29641 ggagctgcta gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta
    29701 aaattgtatg ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt
    29761 atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg
    29821 ggtgaaacct cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag
    29881 agctatgaag cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa
    29941 aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga
    30001 ggaataagat atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt
    30061 catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa
    30121 aggtgttgaa ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga
    30181 aacagttatc caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga
    30241 agttggacaa aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc
    30301 agatatgatt ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat
    30361 cgttgcaggt atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt
    30421 cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga
    30481 aaatgtggat acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa
    30541 gacaccgatg cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg
    30601 tatctctgat ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac
    30661 agtgatggaa aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg
    30721 cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg
    30781 agctgaacaa gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca
    30841 agatgcttca gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac
    30901 atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga
    30961 tgaatcaaaa aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc
    31021 tgctcaaaag actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa
    31081 cagttttggc gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc
    31141 caattttgac aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc
    31201 tcatggcgat gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg
    31261 aagagcacat gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg
    31321 ctcttgttca tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat
    31381 ccgctcaagc tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag
    31441 ataaattatt agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa
    31501 ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg
    31561 cgttggacag tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct
    31621 gttttattga agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc
    31681 ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga
    31741 ctatggcgcc taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag
    31801 aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta
    31861 tggggatgag tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag
    31921 gaacagcgct attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga
    31981 gcaattgcta attttttcgg attagctgat gaggaagagt acgaatacca agattatcct
    32041 gaaacaacaa aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact
    32101 cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta
    32161 acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct
    32221 acaaaaactg tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat
    32281 cttgctggaa aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct
    32341 aaacgaatca ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa
    32401 gcgagaagaa tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa
    32461 cgtgttggag atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc
    32521 gctcaatctt ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata
    32581 ctattgcact attacttcat ttacttcaac gggcagccta tttctatagc attatcttga
    32641 tggtatatgc attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat
    32701 tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac
    32761 ccttggattt cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag
    32821 cacagatcgt tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt
    32881 attctcagaa tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg
    32941 cagatgaacg tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat
    33001 atgcacccta tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga
    33061 ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa
    33121 aaagatgttt gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat
    33181 tgtatgaaat ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa
    33241 ctttagtggg aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc
    33301 gctggcaagt atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga
    33361 ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag
    33421 atagctggac acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg
    33481 agatttataa tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag
    33541 tgaactggac agaaaataca agacctgatt ttgtacttga actattagat attatttcag
    33601 tcagaggtta tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt
    33661 acagggtact gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt
    33721 aactccatta gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga
    33781 cgaagtagat gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa
    33841 tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt
    33901 aaaagatgct ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac
    33961 aagtgcaagc aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt
    34021 agttgcgaac gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag
    34081 aaaaatcttg acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct
    34141 taagaaaaaa acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga
    34201 acaagtgaaa agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga
    34261 cagccatgaa gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga
    34321 tgtaaactca gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat
    34381 tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta
    34441 ttcttaaagc gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct
    34501 ctacttgaga cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg
    34561 tcacagagag tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg
    34621 tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg
    34681 aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt
    34741 gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag
    34801 acaagagttg aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa
    34861 tggtaatatc catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc
    34921 aaaatcaatg tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc
    34981 gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata
    35041 cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa
    35101 acgcctagga gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga
    35161 agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt
    35221 gaaaccaatt tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata
    35281 taaagatgta aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat
    35341 tcttgatgaa gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa
    35401 ccaaggtatt gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa
    35461 gtttgtcgta gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt
    35521 aacagttcta aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt
    35581 ctatgaacgt gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg
    35641 attagtccat actgctccag gacatgggga agatgactat atcgctggta gcaaatataa
    35701 actatctgta gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt
    35761 tgaaggtatc ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg
    35821 agctttgctg aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa
    35881 gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa
    35941 tattttggat gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta
    36001 taatatgatc cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc
    36061 acttccgatt ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca
    36121 tgtggcaaaa ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga
    36181 acttcttcct gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa
    36241 agatatcatg gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg
    36301 accagaatta tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg
    36361 gttcaactca agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt
    36421 tatttctcaa ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa
    36481 catcattgca ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt
    36541 atctagtgta gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc
    36601 agaagtatat cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt
    36661 cgatcctaaa aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac
    36721 cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt
    36781 catgcatatt taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct
    36841 ggattttgct aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat
    36901 gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc
    36961 gcatacagca gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag
    37021 cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc
    37081 tttcatggac ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt
    37141 gattggtaaa tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat
    37201 gttaaaagct ttggatgcag atattgcaca attattgatc gtttcaccag actactttga
    37261 agttatggca gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat
    37321 tacagtggaa aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt
    37381 aggagtagat gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt
    37441 ctatcctgaa gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac
    37501 atgttctttg tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc
    37561 agttatctgg ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt
    37621 taggtcccat tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt
    37681 ttcgaatgac atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg
    37741 ttccatcacc attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa
    37801 ccatttctgc actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta
    37861 cttctttccc attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa
    37921 cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa
    37981 caggaacttg tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg
    38041 tacgtacata gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga
    38101 cgaatgtttc agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt
    38161 gtacaccatc caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt
    38221 cttcgttata aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt
    38281 cggaaaatgc tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt
    38341 gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga
    38401 ggttttcgcc aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa
    38461 aaatattatt ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat
    38521 gatcaatcct gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa
    38581 aagcagactg gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt
    38641 ttgtcataat attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca
    38701 tagccaaata atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca
    38761 attcttttaa tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat
    38821 ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga
    38881 caacttcacg gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat
    38941 gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg
    39001 ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga
    39061 tacactcctt tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat
    39121 tttctttcta tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc
    39181 atattcttcg ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc
    39241 aagcactcgt gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg
    39301 ttcttcatgg acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg
    39361 atgattcaaa tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac
    39421 ttcagcatca tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc
    39481 ccataagcga tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg
    39541 tacaccttga taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc
    39601 actgacagat gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt
    39661 tgtatctcca cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt
    39721 cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt
    39781 gactattttt ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa
    39841 gatgatagag aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat
    39901 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
    39961 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
    40021 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
    40081 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
    40141 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
    40201 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
    40261 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
    40321 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
    40381 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
    40441 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
    40501 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
    40561 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
    40621 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
    40681 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
    40741 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
    40801 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
    40861 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
    40921 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
    40981 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
    41041 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
    41101 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
    41161 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
    41221 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
    41281 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
    41341 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
    41401 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
    41461 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
    41521 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
    41581 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
    41641 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
    41701 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
    41761 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
    41821 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
    41881 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
    41941 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
    42001 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
    42061 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
    42121 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
    42181 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
    42241 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
    42301 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
    42361 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
    42421 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
    42481 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
    42541 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
    42601 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
    42661 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
    42721 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
    42781 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
    42841 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
    42901 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
    42961 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
    43021 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
    43081 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
    43141 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
    43201 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
    43261 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
    43321 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
    43381 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
    43441 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
    43501 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
    43561 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
    43621 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
    43681 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
    43741 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
    43801 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
    43861 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
    43921 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
    43981 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
    44041 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
    44101 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
    44161 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
    44221 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
    44281 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
    44341 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
    44401 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
    44461 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
    44521 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
    44581 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
    44641 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
    44701 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
    44761 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
    44821 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
    44881 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
    44941 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
    45001 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
    45061 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
    45121 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
    45181 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
    45241 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
    45301 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
    45361 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
    45421 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
    45481 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
    45541 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
    45601 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
    45661 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
    45721 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
    45781 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
    45841 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
    45901 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
    45961 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
    46021 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
    46081 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
    46141 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
    46201 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
    46261 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
    46321 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
    46381 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
    46441 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
    46501 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
    46561 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
    46621 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
    46681 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
    46741 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
    46801 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
    46861 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
    46921 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
    46981 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
    47041 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
    47101 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
    47161 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
    47221 atgcttttat cggcacactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
    47281 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
    47341 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
    47401 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
    47461 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
    47521 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
    47581 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
    47641 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
    47701 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
    47761 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
    47821 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
    47881 tgatcattga tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt
    47941 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
    48001 gaccaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
    48061 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
    48121 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
    48181 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
    48241 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
    48301 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
    48361 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
    48421 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
    48481 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
    48541 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
    48601 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa
    48661 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
    48721 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
    48781 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
    48841 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
    48901 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
    48961 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
    49021 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
    49081 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
    49141 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
    49201 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
    49261 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
    49321 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
    49381 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
    49441 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
    49501 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
    49561 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
    49621 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
    49681 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
    49741 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
    49801 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
    49861 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    49921 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    49981 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    50041 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    50101 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    50161 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    50221 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    50281 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    50341 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    50401 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    50461 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    50521 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    50581 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    50641 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    50701 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    50761 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    50821 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    50881 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    50941 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    51001 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    51061 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    51121 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    51181 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    51241 cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg
    51301 gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata
    51361 taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat
    51421 atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
    51481 aagtatattt actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa
    51541 agtaaattgc ttttcaatcc ctaattcttt taattttttg cgcaaatatt tgctgacggc
    51601 gtttgatgaa actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttgat
    51661 ttcaaaattc tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt
    51721 atgtttatca atggaaattt tcctatttga agtttcgttt tttgtttttc cccattcatt
    51781 taatttataa ttccatgttt tattaatagt gatgatttga tcttcgaaat cgatatcttc
    51841 ccatgttaat cctagaagtt cagcaaatcg cattccagtg gcaccagcaa ctaacacgag
    51901 cataggagaa gagtactgcg cagaaaggtt ttcttctacg agtttcatta aagatttaaa
    51961 ttgatcataa tctaaatatt tatcttcttt tggcttcaat gaatcttttc ctttgattac
    52021 tgcttttcta gttggatcaa acggaattaa tccttcttct acggcatctt tcaacgacgc
    52081 tctaatatgg ttattaaact taataacggt agattttaca tggtctttag catacttatt
    52141 taaaaagcgt tgatatccca ctctatctaa atcagaaatc aataccgctg gcatatattt
    52201 ctttatgttc attaacgtat cttcgtattt tatataagtg ataggagata ctgttccttc
    52261 tttataaagt tgcatccaat cctcaaagta atctgataat aatagatttt tgcgatcccc
    52321 tttaaggcct ttgttcaagt tatattctaa ttcgttggcg gcatcttttg cttctccttt
    52381 tgtcttaaat cctgattttc ttatcttcgt gtattttccg tcatcttttt tgtatgaaat
    52441 ttcgtattgc caactatttc cacgtttgac taatcttgcc ataattgaat cagctctctt
    52501 tctctgatac aataggaact ataaagaagc ccattgtttg ggttttatta ttttttgcac
    52561 atcctttttg cttgccggcg caaggatgtg ctatttgtcg ttttaatggt ataattggat
    52621 gtgtattaga tcatccaatc agaggtgttt attatgatta aagtgatttt taaaagaata
    52681 actgaacttc cagagtttag attatttgtt gattattggt atgtctggtt ttttattgtt
    52741 gcattgctga taggtctaga tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa
    52801 taaattgaat tcctaaatga gatattttgt agcccatatt ttgtttgccg acatcaccta
    52861 atactatgga gctaatcgtt ttttctaaca gaccaaaatc agataatcta tttaagctac
    52921 catctattag ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa ttttcgtttc
    52981 cacgactcat taagcatgct aacaattgaa tctcaatcga tgttaattga ttcagtacaa
    53041 gaagataata ctcctcgtta tcccatgaag cgttgttaat tcgaagtaaa caattcttat
    53101 aaagattttt atacatatct attttatttt gtgcctttga tttttctacc tcgtcattta
    53161 tcccttcgaa tattccaatg agttggtcct tgttctctaa gttgaaaaca tcttctggaa
    53221 tttgatcgcg aatgttttct aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt
    53281 tgaaacgctt ctcgttttta gacccaaaat aaagtgactg cacaacgcca ccaatataag
    53341 gaattgctcc aataccgcct tcaactatta attcaagata ttcttttttg gttagttttt
    53401 cattgctatt atccatagtt acctcctatt ttgtcatttc ctttacaagc attgtgactc
    53461 ctgatggcac tcttttgtat aggttttatt attgtgcaca atctaactct tagtggggcg
    53521 tggttcattt attttttact tactttacta ttaatcatat cccattgagc ataggatttt
    53581 tctatgttcg atcctcttga actaatttct cctgtaaaag ttactttatc gccctttgta
    53641 aaggtatcag cattgtttaa gtccttcgca aaaattacat atgaaacttt aggtgttgca
    53701 gaaatatcat tccaactttt atcatcgtac tctttgtctg ctataatggc tattctagta
    53761 gtcattgatt cgataacagt tccttcaaaa gtatatgttt taccactaat atatttagta
    53821 aaagctgtat tttgctcagt agctggagta atagaataat aactatctaa gaaagcaccc
    53881 cagtcatgat tatttgtctc taaaaattct tcaaattgag ttaggctttt attgctgctg
    53941 ttagatgtag tagtttgagt atcagatgat gagctttcct gtttgttatt gtcatttgcc
    54001 tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa taatcccgat aataaggaga
    54061 atcataggtg ttttcttatt tgtttttttg ataaatctta caattaacat gattaaacct
    54121 ataataaata aaacaatacc agtaaaacca aatataccgt ccattaatag atcctctttc
    54181 tcttttgata ttgaatatta taaaccaagc aattcttttt tcttaaaatc gaattcttct
    54241 tgattaataa tcccttcatc taacaactct ttatactttt taatttcatc tgcacttgaa
    54301 atttgtgttt ctttggaatt attatctaat atagattgtt gcttctctat atattttttt
    54361 aaattttcca tttcattcca atattttttt gtaaacataa cggtgttttc atctttcgtg
    54421 gccgctaata tacctccgcg actttcgttt ccgcctagca aggtaaattg tatataacca
    54481 ttactcatac ctggtttttt taattgtacg gctgatatat ttttaaaggg gatagttttt
    54541 tctcctttaa taccttgatt aactaaattt ataaatcctt ttctaataat tgaaatagaa
    54601 tcatcagtta ctttaatcaa ggtttttcct ggagatttta ttaaaatctc tttttccatt
    54661 ttttcttcct catttctgtg atatgatttt ttttgtagaa gctcatatat gaggaaagag
    54721 cccgtgttgc agcacgggct tttttaatgt tttgctgaaa tcggtttttt taataattct
    54781 tgatgtattc atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag
    54841 acagcataac aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg
    54901 agattaaact gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga
    54961 atagctattt tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac
    55021 aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag
    55081 gcttctgcag tttcaccgta tagattttca ttcaaaaaaa tgattctggg ttttggatag
    55141 tagaaaccag gctctttcat ctccatataa actactttca aattgtattc gctcaacatt
    55201 tctttcaatt tcaaatacat acaaaccatc actccaacta ttcattttcc tctaaagctt
    55261 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
    55321 aaacagtatc gtcttctgat aaatccacat gtttaggggt ttctctttct tctctaccta
    55381 gaaggtagtc tacagagaca tcgaaatagt cagcaatccg acttaattca tctgaattag
    55441 gagtattatt tttccacttt gctagataac cgttagaata tccaaaatta atttctaact
    55501 gccgtataga tatctttttc ctcttcgcca agtcttttat tatttcatag gtattcatcg
    55561 ataaaacaac ctttctaaat gtttacaaaa aaagtttaga aaaatacgca gaaataattt
    55621 gactaattct gagtaatgag ctatactata tcttgtaaac agatttaaca actaaaaaga
    55681 caacaaaaaa cactattgat atgtaaatgc agaccgccaa gaaagcttta aaatcaatgt
    55741 ttttatgtct tatttaatta tgctcttagt atagagtatt actcagatac tgtcaattga
    55801 atttagaaaa aagttgttaa atttgtttac gaatataaaa gaaaggaatg agaaatatga
    55861 acacaccgca aatttttaat ttcgaacaaa acgaagttcg gacaatttta gtaaatgatg
    55921 aaccatattt tgtaggcaaa gacgttgcga gtgttttggg ttattcaaac actaaagatg
    55981 ctttgtcgcg gcatgtagat ttggaagata agatggggtc gcgaatcacg acctcaggtc
    56041 aatcaagaga gatgacaatc atcaacgaat ctggtttgta cagtttaatc ttaaaatcaa
    56101 aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag tgaagtgttg ccagcaatta
    56161 gaaaacatgg aggttatcta actccagaaa aagtagaaga agctttgctt aatccagata
    56221 caatcattca attagcaact caactaaaag aagaaagaac tggaagatta atcgcagaac
    56281 aaaagattgc agagtacgaa cctaaaatct cctatttaga tagcatatta tcttctacag
    56341 attcagtaac aattagtcag attgcagcag attatgggat gtctccacaa cagatgaata
    56401 aattacttca taaactaggt attcagaaaa aagtcggtaa ccaatggtta ttgtgcaaaa
    56461 aacacatgaa ccaaggatac acaaaatctc atacaactga gatcccgaaa gccgatggtg
    56521 gcactaaaat tgtaatgaat accaaatgga cacagaaagg gcgtctattt atctacgaat
    56581 tactaaaaaa agaaggatat taccctcaaa tggatttaga ggaaattggt tagaaaggag
    56641 ttttagtatg actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata
    56701 tgtcgaaagt tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa
    56761 aagctactta tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat
    56821 agattcgatt atcactatgt acgaattgta aagggaggaa cagctaatgc aatatctaga
    56881 agcaaaaatc ccaattccag aaggctatgt aattatctcc caagtggatt atgaggagtt
    56941 aaaaaaagct gatgatactg gtagatggat gacgttgcca gaagtgctag aacggattaa
    57001 cagaaaatat gattggttta cttctagagt tttaaagaac ccaagatata gaaatattat
    57061 cgatatcgag aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata
    57121 tctattttta agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag
    57181 gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt cagcgcaccg ctaatcatct
    57241 acgctttagg actttgggga agcaggcaag cgttgatagg aacgattgtt tacatggttt
    57301 ggatttttat ggggcttgat gaagctgaag ctgagtacag agcgaaaaag ccaaccgagg
    57361 gggctgacta aaaatgaata gaaaagagaa actagaatgt atattactat tactcagttt
    57421 aattctttca ctaatttctc tattgggaag tttgtatttt tgatatcaac aattttgtgg
    57481 atttgggttc tatttagagt aaatatcatt tttgtttggt gattaaggag tttattaaaa
    57541 ttaattttcc ctacttcaaa tgcaaggtaa caagatatag cacttttagg aggaatattt
    57601 agcggtacag cttcagagta tattctatta tcatcgactg atcctccttt agcaattaat
    57661 ttcttaaatg gagtagctga ccatctgcgg tttaagttag cggatgaaag ttctaaatta
    57721 actaacatag ctggttctgt tgaatagtta gaaatgatta cttttgtata aatcatatct
    57781 gcagcgaagt aagaagcatt aagttcaact tctagttgag ggcgcattcc ttttattttt
    57841 atccctgtta aaaacgtact caatataaaa cttattattg acatccattg aaaaattgtt
    57901 aagtgaaaaa aattcaaaat aaacaccacc agtttttaac taaattatac caaaaaggag
    57961 agaagaaata atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagctt
    58021 acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt ttaagagata ttgatgcact
    58081 aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa ggtaatgaac cagattcata
    58141 tggcagaaat cgacgagttt tcttcattag tagagatgga tttttcttgc tggctatggg
    58201 gtttacagga aagaaagcta tctacttcaa acaaaaatac attgaagcat tcaacgaaat
    58261 ggaagatgtt attcgcaaga atactgttcc tcaaacaatt gaagatatga tgatctatca
    58321 attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat
    58381 tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta tgggagttct
    58441 agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg
    58501 gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt
    58561 cgatgatgct gtttcattta ttgaaatgtg gttgccagaa actgcgatcc gtatggaaat
    58621 tgatcaactg aacagacaac agagactttt tggtgatgac aatgaataga gctgaagcgc
    58681 taagaatagg gacggcaatt gctaattgct ggtggaaata ctataaacca atcatcctaa
    58741 gccaacaaca tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc
    58801 aagcaaagag tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact
    58861 ttccactcta gatcaatatt tgattgatcc tgattggggc aagccgaaaa ttgaggaaac
    58921 aagtggtcga aaaatcagac gaaatctttt gacgaatgaa gaactagctt gggatcaaga
    58981 tgatttaggc aaccatgtaa ctatttggga tcatgtttat cttatccatc tatcgaagca
    59041 ttcgaataaa cctgaatata tttacgtcat cgaagatggc ttgattgatg cg
//
LOCUS       DACGLH010000010        56151 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.011, whole genome shotgun sequence.
ACCESSION   DACGLH010000010 DACGLH010000000
VERSION     DACGLH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.011"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            176..1021
                     /locus_tag="IXT21_000688"
     CDS             176..1021
                     /locus_tag="IXT21_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4868117.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1302..2522
                     /locus_tag="IXT21_000689"
     CDS             1302..2522
                     /locus_tag="IXT21_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ4868118.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2519..5239
                     /locus_tag="IXT21_000690"
     CDS             2519..5239
                     /locus_tag="IXT21_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4868119.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRFQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5223..6167
                     /locus_tag="IXT21_000691"
     CDS             5223..6167
                     /locus_tag="IXT21_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4868120.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6236..7246)
                     /locus_tag="IXT21_000692"
     CDS             complement(6236..7246)
                     /locus_tag="IXT21_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4868121.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7426..7770)
                     /locus_tag="IXT21_000693"
     CDS             complement(7426..7770)
                     /locus_tag="IXT21_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4868122.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7763..8197)
                     /locus_tag="IXT21_000694"
     CDS             complement(7763..8197)
                     /locus_tag="IXT21_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ4868123.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8335..9072
                     /locus_tag="IXT21_000695"
     CDS             8335..9072
                     /locus_tag="IXT21_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868124.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9065..10276
                     /locus_tag="IXT21_000696"
     CDS             9065..10276
                     /locus_tag="IXT21_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868125.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10483..11262
                     /locus_tag="IXT21_000697"
     CDS             10483..11262
                     /locus_tag="IXT21_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ4868126.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11332..11994
                     /gene="trmB"
                     /locus_tag="IXT21_000698"
     CDS             11332..11994
                     /gene="trmB"
                     /locus_tag="IXT21_000698"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ4868127.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12092..13846)
                     /gene="ade"
                     /locus_tag="IXT21_000699"
     CDS             complement(12092..13846)
                     /gene="ade"
                     /locus_tag="IXT21_000699"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ4868128.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13978..14907
                     /gene="cpaF"
                     /locus_tag="IXT21_000700"
     CDS             13978..14907
                     /gene="cpaF"
                     /locus_tag="IXT21_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ4868129.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14864..15913
                     /locus_tag="IXT21_000701"
     CDS             14864..15913
                     /locus_tag="IXT21_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ4868130.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15910..16188
                     /locus_tag="IXT21_000702"
     CDS             15910..16188
                     /locus_tag="IXT21_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ4868131.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16163..16633
                     /locus_tag="IXT21_000703"
     CDS             16163..16633
                     /locus_tag="IXT21_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ4868132.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16650..16952
                     /locus_tag="IXT21_000704"
     CDS             16650..16952
                     /locus_tag="IXT21_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868133.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16969..17340
                     /locus_tag="IXT21_000705"
     CDS             16969..17340
                     /locus_tag="IXT21_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ4868134.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17312..17671
                     /locus_tag="IXT21_000706"
     CDS             17312..17671
                     /locus_tag="IXT21_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868135.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17843..18850
                     /locus_tag="IXT21_000707"
     CDS             17843..18850
                     /locus_tag="IXT21_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4868136.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18875..20059
                     /locus_tag="IXT21_000708"
     CDS             18875..20059
                     /locus_tag="IXT21_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ4868137.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20496..22079)
                     /locus_tag="IXT21_000709"
     CDS             complement(20496..22079)
                     /locus_tag="IXT21_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4868138.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22657..22800)
                     /locus_tag="IXT21_000710"
     CDS             complement(22657..22800)
                     /locus_tag="IXT21_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ4868139.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23023..23502)
                     /locus_tag="IXT21_000711"
     CDS             complement(23023..23502)
                     /locus_tag="IXT21_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4868140.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23575..23859)
                     /locus_tag="IXT21_000712"
     CDS             complement(23575..23859)
                     /locus_tag="IXT21_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868141.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23937..24131)
                     /gene="ssrS"
                     /locus_tag="IXT21_000713"
     ncRNA           complement(23937..24131)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXT21_000713"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24203..25489)
                     /locus_tag="IXT21_000714"
     CDS             complement(24203..25489)
                     /locus_tag="IXT21_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ4868142.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25627..26298
                     /locus_tag="IXT21_000715"
     CDS             25627..26298
                     /locus_tag="IXT21_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ4868143.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26264..26752
                     /locus_tag="IXT21_000716"
     CDS             26264..26752
                     /locus_tag="IXT21_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ4868144.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26765..27712
                     /gene="prmA"
                     /locus_tag="IXT21_000717"
     CDS             26765..27712
                     /gene="prmA"
                     /locus_tag="IXT21_000717"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ4868145.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27713..28477
                     /locus_tag="IXT21_000718"
     CDS             27713..28477
                     /locus_tag="IXT21_000718"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ4868146.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28710..30923
                     /locus_tag="IXT21_000719"
     CDS             28710..30923
                     /locus_tag="IXT21_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ4868147.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30934..31380
                     /locus_tag="IXT21_000720"
     CDS             30934..31380
                     /locus_tag="IXT21_000720"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ4868148.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31551..32810
                     /locus_tag="IXT21_000721"
     CDS             31551..32810
                     /locus_tag="IXT21_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ4868149.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32899..33846)
                     /locus_tag="IXT21_000722"
     CDS             complement(32899..33846)
                     /locus_tag="IXT21_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ4868150.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34240..35532
                     /locus_tag="IXT21_000723"
     CDS             34240..35532
                     /locus_tag="IXT21_000723"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ4868151.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35547..37316
                     /gene="aspS"
                     /locus_tag="IXT21_000724"
     CDS             35547..37316
                     /gene="aspS"
                     /locus_tag="IXT21_000724"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ4868152.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37425..38300
                     /locus_tag="IXT21_000725"
     CDS             37425..38300
                     /locus_tag="IXT21_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4868153.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38566..40449
                     /locus_tag="IXT21_000726"
     CDS             38566..40449
                     /locus_tag="IXT21_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ4868154.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40524..40808)
                     /locus_tag="IXT21_000727"
     CDS             complement(40524..40808)
                     /locus_tag="IXT21_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ4868155.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40798..41532)
                     /locus_tag="IXT21_000728"
     CDS             complement(40798..41532)
                     /locus_tag="IXT21_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ4868156.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41661..42284
                     /locus_tag="IXT21_000729"
     CDS             41661..42284
                     /locus_tag="IXT21_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ4868157.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42476..43597
                     /locus_tag="IXT21_000730"
     CDS             42476..43597
                     /locus_tag="IXT21_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ4868158.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43604..46732
                     /locus_tag="IXT21_000731"
     CDS             43604..46732
                     /locus_tag="IXT21_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ4868159.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46751..49945
                     /locus_tag="IXT21_000732"
     CDS             46751..49945
                     /locus_tag="IXT21_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ4868160.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50091..50798)
                     /locus_tag="IXT21_000733"
     CDS             complement(50091..50798)
                     /locus_tag="IXT21_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ4868161.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51563..51961
                     /gene="spxA"
                     /locus_tag="IXT21_000734"
     CDS             51563..51961
                     /gene="spxA"
                     /locus_tag="IXT21_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ4868162.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52293..52946
                     /locus_tag="IXT21_000735"
     CDS             52293..52946
                     /locus_tag="IXT21_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ4868163.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53172..54170
                     /locus_tag="IXT21_000736"
     CDS             53172..54170
                     /locus_tag="IXT21_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ4868164.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54346..>56151
                     /gene="pepF"
                     /locus_tag="IXT21_000737"
     CDS             54346..>56151
                     /gene="pepF"
                     /locus_tag="IXT21_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ4868165.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18480 a         9700 c        11647 g        16324 t
ORIGIN      
        1 tggattcaat tcatatttat ttataaatca aaaatacaga gaatagtata taaatgtcct
       61 ttcctattct taaaatgatg tttgtactta cttaaattag gtataattag tatatgtttt
      121 gtgagaaatt tagacttaca ataagcagtg aatttataat caacgtatag gagacatgga
      181 tgaaatgaaa ctaattggta tagatttaga cggtactctt ttgaattctg accagagcat
      241 tagtcaaaaa aatgcaagaa caatgaaaca tttaccggaa gactgttttc cattcatttg
      301 ttctggaaga gaagtagaag acattaataa tattctcaaa aaatctggtt taaggatacc
      361 agcagtaggg ttaaatggtt ctgttggatt tgatggagaa aaaaagatat ttgagtcttc
      421 cttcgataaa aatgatgtaa aaaaaattga ttctgtccta tcgaaatttc caaccaaaat
      481 atatacaaat ctcggaagtt atgaatctca gaattataaa aataaaatga aaaaaatttt
      541 tcacgaggta gggagtgaat tttctatcaa agagctaaac tatgagcttg agtatgaaaa
      601 atctatttgt tcaatccctt ataataatat tgaggaaatt atagaaaagg atgctatcag
      661 catttacaaa ttttttattt ttattcctaa tcgtcaattg aaagctaata tatttaacca
      721 tttgaaaaag atgctaaatg tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt
      781 acctcaaaat gtatctaaag gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa
      841 gaaacctttg agaatcgcga tcggagatag tttaaatgac ttatctatgt ttgaaagtgc
      901 agatattagt tttgcaatgg gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt
      961 cactgtttca aatgataaag acggagtatc tcaagcattt gaaagaattc ttaccatata
     1021 aactaattaa ggtagtatag aacatagaat tctaaatacg gaaaaatgga gcataaattc
     1081 cagtacaaat agtacttctt cagtgtcaaa cgaagcgaat ataacataga tatttaggat
     1141 tgtgacaaaa atcatgtcac aatctttttt tcgaataaac ttattttttg aagtcggcca
     1201 tttatcgtaa tttctttgca taatacgaac aaattttcgg ttattgatga aaaaaaacat
     1261 tgaaaatggt atgataaaag aaaaaaagga ggaaattttt aatggtgaaa tttatccatg
     1321 cggcagattt acatatggat cggtcatttg aaggactgac aactcttgat aaaactgtac
     1381 aggaaaaact attgaagacg aatctgaccg ttttatcaaa tatcattgaa caggcaataa
     1441 taaataacgt tgattttgtt ttacttgcag gagataattt tcatcaaaat cgtccttcat
     1501 taaaaataca gaaacatttt tcagaacaga tgaaacggtt agaaaagaac catatacctg
     1561 tatttataat attcggaaat catgatttct atcaaaaaga gcggtattgg ttctcctttc
     1621 ctaaaaatgt ccatttattt acatccgaaa cggtagagac aaaaaaaata acgataaaaa
     1681 gtggagaaac tgtctctctc agtggattta gttatcgtca accgtggatt caaaaagaca
     1741 aagtgatgga atttccttct agagaactca cagattacca tattgggttg taccacgggg
     1801 aacctggcgt ctctcaaaaa ggaaactatg ctcctttcca accctcaaag atgcaagaaa
     1861 agggatacga ttattgggct ttagggcata tccatgtgcc aacggtctta aatgaaaagc
     1921 aaacaattgt ttatccaggt gcgcctcaag gtcatactaa aaaggaacaa gcttctacat
     1981 ctattctact agttgaatta agtaaaggaa gttgccaaat acatccgatt aaagtagcag
     2041 aggtctattg gaagacaaag gaaatatcat tgaggaaagc aagaacgaca aaagaggtca
     2101 ttgttcacat tcgtcagcag ctttcaaaag tagaggatgg atttagttta attgagataa
     2161 aattaaaaga ttatgaccat ctcaatacag atgttttgga acggatacaa tcaggagagc
     2221 tattagacta tttgttagag gaattctcag ataggataaa tgatttcttt atttggcgta
     2281 tttctctcat agagaatact ttttatacga agaccccact tgctgcttct gctaaattga
     2341 tggaacagtt atttcaatac tatcagacac ctcaagattt ccaacaaatt ttaaatgaag
     2401 tatatagtca tcaagaagcg gctagaatat tgagcgaact gccggaatat caagaagaaa
     2461 cacttgaaaa agccaagcag ctgcttaatc aagatttttt atttgaggag gatcaggaat
     2521 gaagatttta aggatcgaga ttgctgcttt tggaaaatgg cgtcaaaaaa gttttgactt
     2581 ttattcaggc aatcagctga tctatggggg aaacgaagca ggaaaatcaa cgatttatca
     2641 atttattcaa gcaatattat ttggtttccc ttcaaaaggc cgtaaaaaaa aggattatac
     2701 accaaaagac ggctcagcct atggcggcaa aatatggctt aaacatcctg tatatggtga
     2761 atttgctgtc gagcgctaca agcagcaaaa tcgcggaaaa tcgaaggtgt ggctggggga
     2821 tcaagtggga agtgatgagt tactagaaaa actgatcgca cctttgacga aagagttatt
     2881 ccagcaggta tttactttcc agcaagagca gttgatggaa ctagatcatt tgagagaaaa
     2941 agagctacat gatgcgctga tttctttagg catcacgggc agttctcaag tattccaaaa
     3001 gcggcaagaa tattatcaga aagcacaaca attattcaag aaaaaaggcc aaaaattact
     3061 aatcaatcaa aaattaaatg aatggaaaga gctgcaagaa aaaattcatc aaaaacaaga
     3121 acaggaagct tattttggca agttgatcca acaagaacag gaatacgagt cgcaacagca
     3181 gcagttattg gcacaattaa aagaggcgaa caaacaattt tcattagcca gacagcaaga
     3241 acttaatttt tctttatatg aagaatggca atctctcaaa aaaaacagca ctacaggtct
     3301 tcctgatgag caacttcttt tagatctgga gtctttctcc aaacggtatc agcaaacaaa
     3361 tgagcgtttg gaagaattag aagcaacact ggaaaagcat agcgggatgg atcagcagtc
     3421 agccagatat tatttttatt tagagcagga gcaggaacta aaagatctcc agcaacttca
     3481 atttacagcg gaacaattaa acgaagaatg gcaaagatta caaattgaga ctagagaaac
     3541 aaattcagaa ttgaagattt tggaacaaag gtggcattgg cagaaaaatc cgttaccgga
     3601 agaattttta tatgaagaag aatggaagca tcttctggct gaagaaagaa ggattgctga
     3661 tctagaaagc cagatttctg tgcgtttcca aatagctaaa gaacagcaag agatgattga
     3721 agcagaagtg aatgacttgg aagagaccta tccagcatta ttacaaaatc agtcagagat
     3781 agcaccagtc tcttcatcta aaccgtatat gctgatggct ggcggagcaa taatcattgc
     3841 aggtatattt gtaccaatgc ccttgaaagt gatcctttta ctaattggtg caggcatgat
     3901 tggctatttc ttctacaaaa ggcaaccaaa aattaaaact aaaaatgatt tctctgagat
     3961 aaaaggaatg tggcaagaaa aactaggaca acttgatcta gccttagctc aattagctga
     4021 agcagaagaa aaacaaaaga gaactggaga agaaaaacag attcttcagc aagaagtata
     4081 tcgttttgcg gatcatcggc acttaggaaa gatggatacg ctcaaactca tgaaagaata
     4141 tggacaggaa gtcatagatt atcagaaatt atatggcgta tttgagcgga aacataaaag
     4201 agaacaggaa atacaagaaa aactacgaca aatcgaccaa aaatttgagt ttgttaaaga
     4261 atggttaccg atacaagata agacaatttc agaaaaaatg gtattgcttt cccgttttat
     4321 acaagagatg gaacagatga aatttgccag aagctatcag caaaatactt tattgaagca
     4381 agaaatcaat caattgaaaa agaaacaaaa agtatggata caagaataca gcgaacaatt
     4441 gactcaagca ggcttatctt atccttcgga agtacctgtc tttttacaaa aagccaaaca
     4501 agccgaacag cagatgaaaa gatacaatga attatcggaa atgcttactc cactatttcc
     4561 tgaagagctg acaaaagaga aattatctgt aagactgcgc caactgaaag aaaagcaatc
     4621 ccaactccag caagaagaaa aagaagcaag tgaaaatcgg caacgactcc aacttcaagt
     4681 agaagagttg caaagagacg gcacattgga cgaactttat caacaagcta gccggcaaaa
     4741 agcaaaatta caggaattac tggaggaatg gggagcactt gaaatcgccg ggacattctt
     4801 aggtgatctt tcaacagaga tgagtgaaag acaattaccc cagttgttaa gagcagcagg
     4861 tgattattta ggtattttaa caaaaggaaa ttatcagaag atattactga aaaatgatac
     4921 actgacttta acagacggat atacagaatt tcccatctat gagttatcta caggtacgaa
     4981 agatcagctg attatggcga tgagatttgc ttacctctca ttagaagcta accgttcgat
     5041 atgcccgatc atcattgatg atggctggct tcattatgat catcagcgaa agtatcaatt
     5101 agctgaactt ttagctaaat ttggagaaaa gtatcaaata atttgttttt cttctgataa
     5161 agaaatggta agctattacc atgagatgaa ccaaatcgta aaacaattag aaggagcaaa
     5221 taatgaaaaa aattcgtgat ttagtaatcg atgagcagtt tgaacaattt gtcctgatca
     5281 aaagtgcaga tgtaagaatc gctaaaaacg gaaaaaaatt tattgctttt actttccaag
     5341 atacttcagg tactatcgat ggtaaatact gggatgcttc ggaggaggaa atcaataaat
     5401 ttacagccgg cacagtcgtt tttctaaacg gtaaaagaga ggtttatcaa ggaaaccctc
     5461 aagtaaagat tcttcatatg cgcttaacaa gggtagatga gccaggagag cctagtttat
     5521 acatggaaag agcccctttg aagaaagaag aaatggaaga agaaatcaat caattgttgt
     5581 ttgagatcac caatgcgcat tggaatcgta ttgttcgcta tctacttaat aaatatcaaa
     5641 aacaattttt tgagtttccg gcagcaaaaa gaaaccatca tgcatttgcc agtggattag
     5701 cttatcatac tttgacaatg cttcacttag ggaaatcaat cgccgatgaa tatcctgagc
     5761 tgaatcgagc attgttgtat gctggtatca ttttgcatga tttaggaaag gtgattgaac
     5821 tttccggtcc tatgtcgaca gaatatacgc ttgcaggaaa tttgatcggt catctggtat
     5881 tgattgacga agaaataact aaagcctgtc ttgcgttgaa aatcagcgaa gctgacgaag
     5941 atgttctcgt attacgtcat atggttttag cacaccatgg cttattagaa tatggctcac
     6001 ctgtacgtcc aagaataatg gaagcagaaa tattacatca aatcgacaat atcgatgctt
     6061 cgatgcagat gatgttgact tcgattcgtc aaacagaacc aggaaaatac accgatcgga
     6121 ttttcggtat ggacaaccga agtttctatg taccaaaaga tttatagcaa aaatttattt
     6181 ttttcattca aaacaaaaaa agcgagacat aagtcttcgc tttttttgtt ttcttttatt
     6241 ttgatgaatc agttgtttct tcagttgaag attcttttgt tgaatcagtt gattttgtgt
     6301 cagaactctt tgttgaagct gtagtttcac ttgagtcttt cgtacttgat gaagttgttg
     6361 tgaatgcact caacacattt tcaaaggcat catctttgat ttttacattt gcatctttta
     6421 attcttcacc gattacttta gttgtgaatg cattgtcact tagtttagtt tcagtagcga
     6481 tatcttttag ttgatctttg tatttatcca tgtcattgcc tttattttgg tttttcacca
     6541 ttttgacaac gtagtattct gtcgcataag aagtaggatt tgttgtagta ataacatcgg
     6601 agatctcacc gtctttcaat ttgaaagctg cttcttttac ttctgcagga atagttgttg
     6661 tcgttgagtc gaatttaact ttaccgccat cttcttttgt ctctgtatca gttgatttgt
     6721 ctttggctaa tttagagaaa tcatctccat catccgctga ttttttcaca tcttttgctt
     6781 catcttcact tgataatttg atgatttgtg cttctacttc tggatggaaa gatttccaag
     6841 cagttttcaa atcgtcatct gtaatatcta cgtgagcttt caagccagct tcaaaagcta
     6901 aattgttacg aatatattct ttataagtat ctttcgtata tccagcagct tctaattgtg
     6961 attcaaaagt atcacctaat gatttcgctt gtttgtcgta ttctgcatcc acttgcttat
     7021 cggttacttt atcgccatat ttgttattga atactttata aatgatcatc cgttgtacta
     7081 aggattggtt cgatgattca agttttgctt catcatagaa atcagataca gtgatcgttc
     7141 cgcctttcat cgttgcgata tctttattag tatcgcctga acaagctgct agagttaaaa
     7201 cagccaacgc actagtagct gccaaaataa ttgatttttt tttcattaaa atttgcactc
     7261 ctgtaattag ttattatttt tatatattaa ctataccata tcccatttcc tttaaaaacg
     7321 ttgtcgtggt agtttcaaac aaaattcaaa atttcatcac attcctagga aatacgcaaa
     7381 cttttctcac ttatatagtt aaatctgttg agaaaataat cattattata tttgttcagg
     7441 atactctggc agatcagctt ttagtttttc tacttgttca ttgatttgtg tgatacgtgg
     7501 ttcggcttgg aatttaaaat cttcgatatc ttttttgatt gcttcttgga aagcaggaat
     7561 cacagattca gctgtttctt ttgtttcgat caatgcgctt ttaaaatgat tcaggctgtt
     7621 atttaaatca tttgtcagag ttgttgcttc ttccaattct tcgatcagtt tctttcttgt
     7681 ttcattcccg ctcctgggcg ccaataacag acctccgaca gtgccagcag cagctccaaa
     7741 taacagtcct ttgaaaaagt ttttaatcat cgcaggacac ctgctttacg attgtttctt
     7801 gaatagcttt tagttcttcc gatccataac tatcttgatg attgacaaag tgaatagaaa
     7861 aatcatcctc tttgctgtag cgagggacta aatggatatg tgaatgaaaa acagattgat
     7921 aggctagttc acggttgtta ttcaagatat taagaccttt catttctgga aaagcttttt
     7981 ccaatgcttg agcaatctta ggcacacgcg aaaaaacctc tcctgcaagt tcgggatcat
     8041 attcaaaaat atctgctaca tgacgtttag gcacaactaa tgtgtgtcct ttcgttgctt
     8101 gagagatgtc taaaaaagca tagaccttgt cgtcttcata tattttatag cttgggattt
     8161 cttgattgat gattttgcaa aaaatacagt tttccatata aaacatcctt tctataaaga
     8221 ataaatactt cagcttaact ttaccataag ttaaagctat tgtaaggaaa aaaacaaaat
     8281 accgtcattt tcttcgtggt ataataaata aaaataaatg gcggaggaaa aagaatgagt
     8341 ttaattatcg atcatgtcac aggggggtac ggacatctcc ctgtgttaaa agatatcagt
     8401 ttcgatgtac agcctggaga gatagtaggg ttgattggac tgaacggagc agggaaaagt
     8461 acgaccatca agcacattat cggtctgctg gaagcttcaa aaggaacgat agaaatcaat
     8521 catctatcct taaagaaaaa tacggaagaa tatcgcaaac aaattgggta tattccagag
     8581 actccttctt tatatgaaga attgacattg agagaacata tcgaagtcac tgcgatggcc
     8641 tacgatatac ctatggatat agcaatgaaa agagcagaaa agttgttgga gacttttcgg
     8701 ttatctaatc gtcttgactg gttcccagct aatttttcaa agggaatgaa acagaaagtg
     8761 atgatcttat gcgccttttt gatcgagcct tctttatata tcatcgacga accattttta
     8821 ggcttggatc cactagctat caatgcattg cttgaattga tggtagaaat gaaaaaagaa
     8881 ggagccggta ttttgatgtc tacgcatatt ttagctactg cagaaaaata ttgtgatcgt
     8941 tttgtggtct tacataacgg agagatacga gcagacggtt ctcttgagat gttgagaaaa
     9001 gaatttcagc tgccggattc ttctttggac gaaatctata ttgctttaac aaaggagcag
     9061 gcacatgagt agtttttttc aaaaacgttt agcaagacat caaaagaaaa tgatgaaata
     9121 tatgcgttat gtattgaacg atcattttgt tttagtttgt cttttcttga ttggcggtgt
     9181 gggattatat tattctaatt ggttgaaaac cctgcagcca ccgtttgcaa ttggagggct
     9241 cataatctct gtattttgga tgctctgttt attcgtgggt aagatggcta cttttgcaga
     9301 ggcagcagat attgtttttt tacttcctaa agaaaaagaa atgcaaacct atttggaacg
     9361 agcttttcgc tattcttgca tttttccgtt gatctttttg atattggcat gcggattggc
     9421 catgccatta gtggtggtta gtacggagca atccttttcg atgtttttcg tctatctgat
     9481 catattatgg agtttgaaat tcagtcatct gcaaatcaag agagcagaat tgtttcgact
     9541 agatcaacaa gttattcgtt attggaaatt aggctggttt ttttcttctc tgatcttttt
     9601 gtttctatca ttttattggc taatatgggc aggaatagca ggtgcattag tacaggcaag
     9661 tctatattac tggtttttgt ggaagcaaca agaaaaagga ttggattggg agaaaatggt
     9721 gcatgtagaa gaacataggt tgcatcagat ctatcaattt atcaatttat tcacagatgt
     9781 tcctgaaatt acggcaaaga tcaaaagaag acgctatttg gatcctatat tgactcatat
     9841 taaaagagaa tcaaaaaaca cttatcttta cttgtttgca agaagattgg caagaggttc
     9901 tgattttagt ggtctttatt tccgattagt aattataggt gggttattga ttgccggagt
     9961 agtcgacttt tattttagtc tgattatcgg tttcttattt ttatatttga tcggtttcca
    10021 actgttacct ttagcgaacc agttccgcta tatggttctc gttcagcttt atccaatcca
    10081 taagaatcag aagaagcagg cgattcagta tctactcaca tgggtattaa ttatcgcgtc
    10141 ggttattttt ggtttgattg ctgcagtcgt attagatggg cagcagcgat gggttccgct
    10201 agcggcttat ttgattgtta ctatgctctt tgttaaacta tatgtaccaa ctcgtttgaa
    10261 gaagatgtca gactgaaaac aaagctgtgt agggtaaaaa tgagaatgaa aaatagagcg
    10321 ttgacagtaa gaatgctgtt cgttctattt tttttgagaa aagctggttt tttgaatggc
    10381 aattaaatga aaaagaacgt atatgtgctt gacgtgtagt gttcaggcga ctagaataat
    10441 aagtactgca aaacaaagag taagaggacg ggagaatgca tgatggagtt tcagttagac
    10501 caagagtggc ggttacgtcc catcaaaggc gacacaggaa aaacatacat aggcatgcgt
    10561 gatgaggaca aagtctttat taaacgaaac acgactccga tgttggctgc tctttcaagg
    10621 gaaggaatta caccaaaact tgtttggaca aaacgaacgg ggaatggcga tactttgaca
    10681 gcacaagagt ggctggaagg tcgcttgctg actcctaatg agattggtca aagaaacgac
    10741 gtgattgatg ttttgtacca tttacatcat tcaaattctc ttaaaaccat gctgacccgt
    10801 atcggtggtc gtgtcatgac gccggagaag atgctgcaaa aatatgaaaa agaattaccg
    10861 gaagaactcc agaaaaatca atttctgcaa attgtccatc gttttcttcg tgagcagatc
    10921 ccgtcaatag ataaggaaaa cttatcagta gtacacggag atgtaaacca tcgtaattgg
    10981 ttagtatgta aaaattattt atatctagtc gattgggatt caatcatgtt tgcagaccct
    11041 gcggtggata ttgcaacgat tttaggaaat tatgtgccac tgtctagctg gaatcagtgg
    11101 ctgatcagtt atggcatacg cccgactgac gaaatgcttg aaaaatttca ttggtatgcg
    11161 gccatgaatt tgctccaaga aatcaccaga tattgcaaac gtgaagatca tcgacggatg
    11221 aacgaagaaa ttttacaatt aaaacgaatt ttcagtggct gaggtttggc ccaaactctg
    11281 cggagatttg gccagacctt tttaattaga ggaaaggaat ggagataaat aatgcgtgtc
    11341 cgaaatcgtc cgggagcagc ggaaatgtta gcggctcacc cgaattttgt aattagtgat
    11401 ccaacattgt ggaagggaaa atggaatgaa ttgtttgaaa acgatcatcc aatccacatt
    11461 gagattggta tggggaaagg ccagttcatc actggaatgg caaaagccca tcctgaaatc
    11521 aattatattg gagtagaaat gcaggtcagt gttgtttcga ttgcactaga caagttgatc
    11581 gaacaaccgc ttcctaacct gaaacttcta catgtggatg gatcagcgtt gacagagtat
    11641 ttcgctgaca gcgaagtaga tcaaatctat ctgaattttt ccgatccatg gccaaaaaaa
    11701 cgtcatgaaa aacggcgatt gacatataag acttttcttg ctgtagatga acagattctt
    11761 cgtccaaatg gagaaatcca tttcaaaaca gataatcaag gattgtttga gtattctctg
    11821 gcaagttttt ctcaatatgg catgatcctc aaacaggtat ggttagattt acatcaaagt
    11881 caatttgaag gcaatattat gacggagtat gaagaaaaat tttcttcaaa aggtcaacgt
    11941 atttatcgag tagaagctca atttcaagat aagaccaaaa agaaaagtct gtgaaactaa
    12001 ctttctgctt tcataaaaac attaatatcc ataaataaat cccagaaaat gaacattaca
    12061 gatgtaatgt attttctggg atttatttga tttaaaatgt atttatttca tcggtttttc
    12121 cacaaggtgg aatcaattgg aagcgagaaa atgaaaataa tcctttgtcg gtgatcttca
    12181 attcgggtat gacagataat gttaagaaag aaagtgtcaa aaaaggatca aaggttttag
    12241 ctgctcctag ttgatgtgct ttagttatta attcattcaa ctgttcatag acgatcgaaa
    12301 aaggttcctg tgtcatgatt ccgcctatag gaagagaaac acaagctatt tcctgctgtt
    12361 tgttgacaac ggtcatcccg ccaccttttt gaatcaaaag attagcagca tacagcatat
    12421 cctcgtcgtt tgtcccaacg acaactagat tatgcgagtc atgggcaatc gttgtcgcta
    12481 tcgctccttg tttcagctga aatcctttca ctatgcctaa tccaatgttt cccgtttgat
    12541 ggtgacgctc gatcaccgca atctttaatt ggtctttttc gattgaagga atgaatcgtt
    12601 cgccttgtcg gtccactttt tctaccaaat ctttagtcac tagactattc gggataattt
    12661 cgatcacatg ggcttgtgaa gaagaaagaa gcagctctaa cgaatgtgga tttaatggtt
    12721 gagcgtttag ttttggcaga caacacgtat atgaatttga atgatcagca aataaatggt
    12781 cttggagttg cccagattga acaactaatg ttcccttata gaaaacttca tgaattggca
    12841 gcttggtaag atcgtctgtt aagaagaaat cagcttgata cccaggggca atcgcaccta
    12901 gtgttttcaa atcaaaacat tctgctgcat tcaatgttgc catctgaatg gcttgtaatg
    12961 gatcgattcc aagtgcaata gcttgacgga cgatatgatc gattgatcct tcttttacta
    13021 aatcattgat cagtttatca tcggtgacaa agaagcatct tcttgaattt tcaggtgtga
    13081 tcgccggtag tagcgatgcc agatcttttg caacggtccc ttctcgaacc attaaataca
    13141 taccaagatc caaacgttct ttagcttctt ttgcagttgt agcttcatga tccgtccgaa
    13201 tacctgctgc aggataaaca tttaactctt ccatgccgat accagctgca tgcccgtcaa
    13261 tttttttctt atgctggtgc atcaattgga tcttttctag gatgtctgtt tcattatttg
    13321 ctactgcttg atagttcatg acttcagcta agcctaaaac ttcgggtttt tgtaaaaaag
    13381 gggctaattt ttttgcattt aaatttgcac cattggtctc aaaaggggtt acaggtacac
    13441 aagaaggtag cataaaaaag atattcatga gtgtttgtct tgcatcttct atcataaact
    13501 caagacctgt actcccagca atgtttccta tttcatgggg atctgtcact acagtagtaa
    13561 ctccatgaag caaggatact tttgaaaatt cgcttggtga taaaagtgag ctttcgatat
    13621 gcacatggcc atcgatgaat cctggcacta gatattgccc attagcatta tgtacgcttt
    13681 ttccttcgta ttcaccgatt cctacgatca ttccttcgca gattgctaca tctttttcca
    13741 tgatttcttt cgtaaagacg ttgacgatct tagcatgctt gatcactagg tcagcaggct
    13801 tttttttact tgctacttcg atatatttcc ttaatgttgt ttccaatgga gtgcctccca
    13861 agataaattt ttataagtat aacataaatt cggtttttgc caatattttt ttgtaaattc
    13921 attttatttg aaacaaaaag atcaaaaaga cgtatgtttt ttgtggaggg gagaataatg
    13981 gaaataaaac gattatcgga tcaaatgata agtgaaagtt ttgccaaaca tgcccaagac
    14041 gtatatatat tgccagcttc agaagtattt tacatctatt ttcgctatgg aaaaaaacga
    14101 acattctgga agaaagtctc ttcggcagat ggacagcaac tgatctcaag atttaagtat
    14161 ttgggacaga tggatatcgg agaaaaaaga aaagctcagt taggtgcatt tacttattct
    14221 ttagaaaaac aggaagttcg tctgcggttg tcttctgtag gagattacct tcaacgagag
    14281 agtttagtga ttcggctatt atacggtact tttgaaacac cccatcgttg ctttagttct
    14341 cgagattttg caactatttt acagaaaacc gtcagacgtg gattatacct tttcagcgga
    14401 ccagtcggat caggcaagac ttctctaatg tatcggttgg ctttggaaga aaaattgcaa
    14461 gtgattacta tcgaagatcc tgtagagata gaagaaccgt tttttctgca gctacagatc
    14521 aatccgaaaa tagcacagac ttatgataca cttttgagac tcgcattacg gcatcgtcca
    14581 gacttgctaa tcattggcga gataagagat gaatcaactg cacatgctgc ggtaagagca
    14641 gcactaacag ggcaccgggt ttttgcaacc gtacatgcac gcaatcttga aggtacattg
    14701 gtcagaatcc atgaactgac aaagaaaaaa gaagagctat ctgaatgttt agctggtgtc
    14761 atttatcaag aacttttgga agatgccaat caccatcctt ctgttttatg gagttatcaa
    14821 tttatggagg aagaatcagt aaaaaaatca tgggaggaaa gttatgaaga agctcaggaa
    14881 aatatttggc agtcagaact attctaaagg attaacaaga agccagcaaa accagtttgc
    14941 acaaaccatg ggagaactgc ttttatctgg ttttagtctg caagaatccc tttatttatt
    15001 actcacgatc cgttcgtttc cacctgttat tttggcaaat atccaacatc agctaaagga
    15061 agggcaagcg tttagtgagg tactttcgca aataggatat ggagcagagc aactggtaca
    15121 gatcgagtta gctgaacgtc atgggaatct gccgacaact ttgttggaaa ttgccaaaca
    15181 gtttcggctt gttgaaggat ttcgcaatga tttgaaaaag ctgcttgcat atccgatgct
    15241 attgctcacc tttttgctag gtatcttgtt tagtatgcgg ttgttcgttt tgcctcagct
    15301 tattggttca ggaatggtca tgcaagagca ttggggtatt cagctgatac aagtctttca
    15361 ttggtatgtt ctactattca tcctagcggt tactctattg gctttactag taaggtatcg
    15421 tctgatgaag ttgtctcctg ttgatcagag tgaatggata agcagacaat tattgattgg
    15481 accgatatat tccttgtatc aatcggctta tttgtcacta gagattggaa agctttttca
    15541 tgaaggtttc gaattaaggc aaatcatagt ctgcttaaag gaaacgaaac aagaaagtct
    15601 gattcagcat cttgcctcta agttgattca aggattggag gcaggtatgt cattggctga
    15661 gcagttccag aattatcgtt ttctatcaga agaattcagt aaaattgttt tacagggaga
    15721 ggcaaaggga agtttaggta aagaactgat tttttacagc gaatggacaa gaaaacagct
    15781 tttcaaacgt cttcatcact ggatgcattt tattcagcca atcatttttt tgggcgtagc
    15841 ggctttgatt ttattagttt atgccgctgt tttattacct gtttacggaa gcatagagga
    15901 ggttttgcca tgaaaaagag aaaaatatct tattatagcg gttttacatt gatcgaaatg
    15961 ctaatcgtct tactgatcat cagtgtatta gtgttgttgt tcgtccctaa tttgtcgcgt
    16021 tataggaatc atgtagacca agaaagtcgt gaagcgatca tccagttggt agacacacaa
    16081 aaagagctct atgcgcttca aaataatgga agagttccta ctgtggagga attgttgaat
    16141 gaaggctata tcaaacgaga acatgcagaa atctatcagc ggccttgaag gtaaggtttt
    16201 agcatcagga tatacgttgt ttgaatcact cttactgatt ttattactta caacgatcct
    16261 ttcttttcca gttctcgcat tttctgcatg gaagaatgaa ttggcagtct atcaattttt
    16321 cggccaattt gaaaaacgaa tctattctac gcaaaaaatc gctattgtga atcagatcca
    16381 aacaggcttt tattggaata gtgaagagaa tcaaatcatt tttcatgttc cgaatccaga
    16441 taaaataaat tgggaagtat tagatattcc tgatgagatt accttgaaac gccatgcttc
    16501 tattactttt gctgctagaa ccgggaatga aagttcacta aaagcttatc aattttattg
    16561 ggaagaaaaa gagcggacga ttacgtatca attccagata gggagtggtc gctatacaaa
    16621 aagaattgaa taaaacagga tttgttttgt tggagtccct tacagcgctt atgatcgctt
    16681 ctgtctgtgt ctacttagtg agctttggcc agctgttttt gcttcaggca gaaaagaaaa
    16741 gtggggagga actgctattt attcgaatga tttatgaaga tatcaagacg cagcggatgt
    16801 atcacaaaag accagagcga agggaagaaa tgatcccata cgatatccaa attgtcgata
    16861 ataatggagt aatcgaaaaa gtgtttgtca atatggagaa agaagcgatc agtattgaaa
    16921 aaacaagtaa ttccagcatt tacgctgatt gaatgtctaa ttggactgat gatattgagc
    16981 agtctattgc tcacattcaa tcttttgatt cagcaaacca tccaagtaag tgaatttttg
    17041 cagcgtaaag atcaaaaaga gtggctaata tttttagcac aattagaaga agaactcaag
    17101 agcagtcaca atgtctctgt gaaaaatcag caactgcact acacgatcga ggataaacag
    17161 tatatcatcg aaagatatca ggcaatgata cgtaaaagaa gaacttccgg cggacatcaa
    17221 cctatgttaa caagtatcac tgagcttcaa ttattggaaa aagaacacac aatttctttt
    17281 tatgtacact ttgaaaatgg agaagatgga tatgccatat ggacaaaaaa cgatcagtag
    17341 aaaatgttat aagggctcta ttttattttc tgtcttgctt ttatttcttt tatgtacgtt
    17401 tttattgatg gcagcattag aaagctaccg tttgtcaatg gatcttaata tacgaatcaa
    17461 aaattattat atagcaaaaa taatgaagca gatggcttta tcggaacagt ctacgcttgc
    17521 cgaaagttcg gaaggagttt tttactacac aactggatct gtaacatatc agtgggtaca
    17581 acagtcccta ttcttagagg tggaagtctc accgtttatc tttcggttca cagaagaagt
    17641 aaaaaatgat acagatacta gtacagaatg atggaatgtt cgtgaaaacg ccctcttttc
    17701 tgttataaca ctgtttttaa taaaaaaatg tttgccgttt cacaaataaa aaacagtgaa
    17761 aacgattgaa attttgtcaa ttgttcgata gaattaaaca ggtgaaaaaa ttgttataat
    17821 atatctagaa atgaggtgca ccatgttacc agaaaaaatg gaacaagggt ttaatcaaaa
    17881 cctggaagct attcagcttt tacagaatgc attgggaact tcctttttag aagcctatgt
    17941 cgaaaatgca gaaaatttaa tagatgatta tcaagtacgt gtggtggatg gagtgccgac
    18001 aaaagagaca acgcaacgga tcacagctct ttatgaagag ttgaagaaac tttctttcga
    18061 gccagaagaa tggcgcagac tgtctcaatt acttttattg aagggcagcc agacagaaca
    18121 tttgcaggcg aaccatcaac tcactcctga tagtatcggt tttttatttg tttttcttat
    18181 tgaacaacta tacacaaata aaaaagcacc ggttaagatt ctggatattg cggcaggaat
    18241 gggcaacttg ttattaacag ttcttttaaa cctatcgaat gcggggtacc aaacagaagg
    18301 aattggtgtg gatattgatg atacattgtt ggctgtcgca gcttctacaa gtgatttgac
    18361 acaagcaaat gttcaatact ttcatcaaga cgggttgcag gaattattaa tagatcctgt
    18421 cgattttgca atcagcgatt tgccaatcgg ttattatccg aatgacgaaa aagcaaaaga
    18481 gtttttaaca agtaccgaag aaggccatag ttatgcacat catttattgc tagagcaatc
    18541 aatgaaatat gtgaagccgg atggttttgg tttgtttttg atgccgagtg gatttttaga
    18601 aacggatcaa agtgaagaaa tcaaaaaatg gtttaaagaa gaaggttatc ttcaaggaat
    18661 gattcaattg ccagatgaat tgttccgtaa taaacaatcg caaaaaagta ttttgatctt
    18721 acagaaaaaa ggaccacaag cacaacaagt aaaagaagtc ttgcttgtga aacttgcttc
    18781 attaaaagaa ccagagaagg tgacggaatt tttcaacgag tttaaaaact ggaaatcatc
    18841 tgctctgtaa tcattcaacg agaaggagag tcatatgtct aaaacaattg caatcaatgc
    18901 tgggagttca agtttgaaat ggcaattata tcaaatgcca gaagaaaccg tagttgcgaa
    18961 agggatcgtc gaacgtatcg gtttacaaga ttctattttt accatcaagt acggcgataa
    19021 tcaaaaattc gaacaaatca tggatatcga aaaccacgaa gtagcagtaa aaatgctgtt
    19081 agatcagttg attgaactgc aaattttaag ctcttatgat gaaatcaccg gagtagggca
    19141 ccgtgttgtt cacggaggag aaacttatgg ggattcagta gtgatcgatg agaaagtaat
    19201 ggaccgcatt gctgaattag cagaatttgc tcctttgcat aatcctgcaa acttaatggg
    19261 gatcaaagcg tttaaaaaga ttttacctga cattttgagt gtggctgtat tcgatacatc
    19321 tttccactca acaatgccaa agcaaaatta tttatatagc ttaccggtgt cttattatga
    19381 agatttcaaa gtaagaaaat atggcttcca tggcacaagt catcgttatg tgtcggaacg
    19441 tgcggcaaaa ttattgaaca aaccgatcga agaactaaaa atcatcactt gccacttagg
    19501 aaatggtgta tcgatcacag cagtcgatgg aggtaaatcc atggatactt ctatgggatt
    19561 cacaccatta gctggggtga caatgggtac acgttcaggt gatatcgatc cagctgttct
    19621 cccatattta atggaaaaat tagagattga tattgatgaa atgatcaatg ttttgaacaa
    19681 gaaatctggt ctgctaggcc taacaggatt gtctagtgat atgcgtgatc ttgaaaagaa
    19741 ctatgaaaaa gaacatattc gtttagctta tgacattttc gtggaccgca tccgaaaata
    19801 catcggcggt tatgtgacaa cgatgaatgg tgttgatgca attgtcttta ctgctggtat
    19861 cggcgagaat gacggacatg tacgtagtga aatcatcaaa ggtatgtctt ggtttggttg
    19921 tgaaatcgat ccagcgttga atgaattgcg tggtgaagaa gtagatatct ctacaaaaga
    19981 atcaaaagta agagtattag tgatcccaac agatgaggaa ctaatgattg cccgtgatgt
    20041 ggagcgtctg cgcggctaag ccaaagcatt cgttaggata aaagatgaaa cgcatgcaag
    20101 agggaattct tgcatgtgtt ttttttgcgg agaagaccgc aaaatggtaa tttaacttca
    20161 atttcccttc ttttttacag gatcttctta gggaattttg cttggaaaag tggtcaaaag
    20221 ggaaaagact ggcattttat ttattgattt tcaggaaaat cccaatattt tattttcatt
    20281 catctttata agggacatta catatgtaaa aagaaaaaac atttttagat cagcctcgtg
    20341 ggctgtcgac tttattcttc gctttatgtt tagctagaaa accgatccga aacattcatt
    20401 gatcgaataa taaaaacttg attttccacc tgtactttgc ctgaatcgtg aaagcacagg
    20461 tatgaaaatc aagttttttg ttgcgaaaaa acgtcctagt caatcagttg gccgagcggc
    20521 tggcggctga tctgctcctg atcaataacg agtgcttctt ggaagtgttg ctgcaaaaag
    20581 tagcggtcat ggctgactgc taggagcgtt ccttgatagt cttgcagcat ttcttcaatt
    20641 tcttctcgag catagctgtc gaggtgattg gttggttcat ccaaaatcag gacgttgatt
    20701 ttttctaaaa agagtttcag caacgccaaa cggatttttt caccaccgga taaatctttg
    20761 atccgtttac ccacatcgct ttgataaaag ccacttttgg ctaattgccg tcgagcgact
    20821 tcttcgtttc cagtcaatgt gatggcgtaa gccagcaaac ggctatcggg atcggcaaac
    20881 tgcagctgct gcggcaaata accgatttgc aagctactac ctaatttgat ggtgccaccg
    20941 taaggctttt cctcaccgag taataatttg agtaaggttg attttcctgc accattgtcg
    21001 ccgatgatgg ctaaatgatc tccacgatag actgcaaaac tgctgtcttt gaatagtact
    21061 tgatcagcgt actgctggtg caagttttga atgatgaaaa cttctttgcc agattttcca
    21121 ccattagaca agctttgcag ccgatttttc gggggaggtg gcggtttgac gagtgtcagt
    21181 ttcgccaggc ggcgttccag ttctttggct tttttgaaga aggactcatt gtctccttca
    21241 tgtgcccact gacgaaaacg acgaatcatg actttcaacc gctggacttc tttttgctgc
    21301 agttcgtaat tttttgtcag ctcagccaag cgagcttctt tcaagactcg aaaacggctg
    21361 tagtttccgg gatactcaat caatgcacct tcttccagtt cccatgtttt tgtggtgact
    21421 tgatcgagaa attgccgatc atgagaaacg accagaaccg atttttttgt attctgtaaa
    21481 tagttttcca accagcgaat actgttaaga tctaaatgat tggtcggttc atcgagtaaa
    21541 agtaaatcgg catcggctgc taaaattttg gctaaagcga ctttaactcg ttcgcctccg
    21601 ctaagttctg ttaaccgtgc ttcctgacgg tgtggaatcc ctaagccttg caagatcgct
    21661 gcaatccggt cttctaatac gtaaccgcct agttcttcaa agcgatgctg cagctcaccg
    21721 taacgggcca gacacttctc taagtttgta tctggttctg tcatcaatgc ttcgtagcga
    21781 ctcatttgct gtttgaggtc ctgtatctca gcggcagaat ggagcaagta gtggttgacg
    21841 gtcgcttcgg aaactgtaag ttcttgttcg accgtcccga tctttaagcc ttttttgcga
    21901 ctgataacac cgctgtcgac accttctctg ccggtcaaga tttgtaaaaa ggttgatttg
    21961 ccagttccgt tgataccgac taaggcaatt ttttcaccag cttcaatttg acaggataaa
    22021 tcctcaaata atggattccc gccgattgtt tttttaatag aaattgcttg tatgatcatg
    22081 atctctctcc ttgagaccac tcaaaaaggc catggttatc catggcctgt gttatttaaa
    22141 ctcagattat catgagaaaa ataccttgag atggccattg attttacgtg aattcaactg
    22201 ttttggacaa acttgtcccg tcaacagcaa ctgtaaaacc aattgcatgg ctaaatgcga
    22261 tatggtatcg ttggtaattt tcattacttg ttattttagt catgcgcatc atagtatttt
    22321 ccctccttcg tgtagttgtc ttttccagtg tacctgaaag tgtcagaaag ttcaactagc
    22381 gctgacattt acttaatatt tatcgatgtg taattctgtc agagagcttt ttttatctga
    22441 ctgagggtaa tgtctcgtgg accacatcat tgtattctga gctaaaccag cgttcaggcg
    22501 aaatcatatg gtgtttgata aaaaactttg tctacatgac ttccttttgc catttgctta
    22561 ataccgttgc attaaacagc tcttctcttt ggaaatctca ccaacatcgc gttcaggagt
    22621 atcgttgtgg ttgcaatggc aagcggtagc ctttcatcaa ttcttgaatg atttaccgaa
    22681 tgtagcactt gccttctcgc ggtacccgaa cccgaaataa aattttagtt tggcgataaa
    22741 atcggtatcc gccatcgata cttcatattt tttcgtatca gattcatcgg aaacttccac
    22801 attgacgaac caataagctt tggcatgctt aggtcgtttt aaaccacacc agggcgacta
    22861 atcgccctgg tgttcacttt tttttatttt acttgaactc attgactgtt ttccctttat
    22921 tttaaacatt ataaagaccg atgagtgctt gccgcgtctt ttgtttttat cacggttttt
    22981 tgtgcaaaaa aacctgccga tagttggcag gtcgtctacg tgttaagctt tggtgtggtc
    23041 tttagctggc aattggtttt ctaaatcagt tcggacaaca agcacatcgc aagctgcgtt
    23101 tctaataaca tattcagaaa cagaaccaat aaataaacgt tctacagcat tcaatcctgt
    23161 ggctcctaac ataatcaaat ctacttgatt atcttctggg atttctctgg caataatttg
    23221 ttttggtgag ccatattcta cgacagaggt tacattattc aagccagctt tcttcgcatt
    23281 gctttcataa tctgccaatg tttgctttgc catttcagta gcttgttctg caagcattcc
    23341 atcaaaggat gaaacggttt ggaaagaacg tgtgtcaatg acatgtgcta atagaagttc
    23401 tccgttattt cggatagcta cattgaccgc ctttttaaaa gctaattcag cttcatcaga
    23461 accatctacg gcaaccatga tttttttgta ttgttgtaac ataagcattc actcctcaag
    23521 tcaatcgtat tgtactcttt tattgtagaa cactttaggg aaaaagagaa ttatttactg
    23581 ctttggttca gcaatttttt ttgcaaatcg gatactgaag gtggcagaaa caataattac
    23641 gatcagtaaa aagaccaagg caataaagcg ttgattgaaa aaagctgtta aaatacccat
    23701 tacgcctaaa acagcatatg cagctccgta aaattgtagg aatccttgat ttttatcatt
    23761 tttgttgatc aatacaaaaa agatatcctg tttattaaat aaataatagc taacgaataa
    23821 taatactaaa gtggcgataa tcattaaaat cctaatcatg aacaaatcct ccaaaataag
    23881 caacaaatag aaaaagcaat ttgccaagag caaattgctt tattctttaa aagatggtaa
    23941 tctccgttaa tgccgttgtt tgtcctgtag tattgatccc gcgaattcaa gcaggtgggt
    24001 cccatggtcg tagcttcgaa ctaccaaatg ataaggcatg ttaccagcag tgacacaaga
    24061 cgggtcccaa gatatcaata aaaatgttcg gtcaacacac aaagaggaca attcgaacat
    24121 cacagatttc ccacctctat agtagcatat gaatttaaaa agagcaatga gagagctcgc
    24181 agaaacagaa ccgttttctt gtctagtttt ttggaggatg ctttttccac tcttttatac
    24241 ggtaatactg ttgacccaga gcctgttcat atttccctgt atcttttggc tgataatact
    24301 gagcattctt cagtttatct ggtaaatatt gctgattgac ccaagcctga tcaaaatgat
    24361 gaggatactg ataacccacg ccacggttga gtgatttcgc acctttgtaa tggctatctc
    24421 tcaaatgatc aggaacatct ccagctttgc cctctcgaat atcagcaagt gctgcatcta
    24481 atgccatgta ggcagaattg gattttggcg acaagcaaag atcgacgacg acgtctgcta
    24541 gtgggatacg tgcctcaggc aatcccaatt tttcagcagc taatacagcg ttcactgtac
    24601 gagccgcagc agcaggatta cctaaaccta tatcttcata accgataacc atcagacgcc
    24661 ggcaaatact tgccagatca cctgcctcaa ctaaacgggc gagataatga agtgcagcat
    24721 ccacatcact tccacgaatc gatttttgaa aagccgaaat aacgtcataa tgagcatctc
    24781 catttttgtc atgggtaaga gcttttcttt ggacacattc ttcaatgatc gacagtgtaa
    24841 gatgaatcct tccttcttta tctgaaggag tcgatagtgt agccagttcc agcccattca
    24901 atgcactccg caaatcgccg ttcgttgcac gcgaaaggtg aagaagagca tcttcgtcta
    24961 actggattgc ttgttgtcca aggccgcgtt ctttatccct taaagcatgt tcgactgcca
    25021 actggatgtc ttgttctgtt aatggtttga cttcaaagat ctgtgtacgg ctgcggatcg
    25081 ctggattgat ggtaatataa ggattctcag tagtcgctcc gatcaagatg attcggccac
    25141 tttctaaatg aggaagcaga aaatcctgtt ttgttttatc taatcggtgt acttcatcca
    25201 ataagaggat cactgtacca ctcatttttg cttcttccgc tacaacttgt agatcctttt
    25261 ttgaatctgt agcggcgttt agcatgcgaa atgcataatt agtagagccg gcgatcgcac
    25321 ttgcgatact cgtttttcca gtaccaggag gtccatatag aatcatggaa gagagcatgc
    25381 gagcatcgac catgcggcgt atgatttttc cttccccgac tagatgctgc tgaccaacga
    25441 cttcatcgat cgttcgaggt ctcatgcgat aagccaatgg ctgttgcata ggattttcct
    25501 ccttcttgtt tttacgtccc ttaattatat catatttagc aagatgccta gaaagaacag
    25561 ccttctgttg ttttgttttc atagcttatc caactaatat tgtgtatgat aaagtaggtg
    25621 agaaaaatga cggataaaca aaagatagat gaattttttt ggaataaaac gacgccagaa
    25681 gtggcacggg atttattggg gatgtatttg gagcatgaca caccagaagg aagattagct
    25741 ggatatattg tagacgcgga agcataccta ggtccagaag acgaggccgc ccatagctat
    25801 ggtttaagaa gaacaccgcg tgtgcgggca atgtatgaaa aaccaggtac gatctacctt
    25861 tacacgatgc atactcatcg gattttgaac atcattactc agccagaagg gattcctcaa
    25921 ggagtaatga tacgtgcaat cgaacccgcc gcaatgattg atcaaatgag taaaaatcgt
    25981 ggcggaaaaa ctggtccaga tatcagcaac ggtccgggaa aattagtgga agcattagca
    26041 attcctcaag aactttatgg tcagtctata gcagatagtt cgttacgctt agtttttgag
    26101 aaaaaaaaga caccaaaaaa gatcaatgct ttgcctagaa tcggtatccc aaacaaaggt
    26161 gtatggacag aaaagccgtt gcgttttgtt gtgtcgggaa atccttatat ctctttacaa
    26221 agaaaaaacc aaatcgagaa agattggggt tggaggaaag aaaatgaaaa agaaggatct
    26281 attaacatat ttagatgaaa taattgaaaa acaagtgaca gactatgatg tagctttaga
    26341 ttgggataca aaaaatcata cgatcgagat cgttgttcgc ttgtttgcag aaaataaagc
    26401 acaatttgag attgatgatg cagaagggat cgtttcgcaa gaaccaatta tcgaatttga
    26461 agacggcgta cttcttttta atcctcagaa atctgttttt gatgaacgca attatctagc
    26521 agtaatccct tatgaaggaa aaaaagggtt ggcaaaatca gtagctgatt ctttagtgga
    26581 atatctaaat gaagtattag ctcaagggca aagcgatttg cttgattttt tggatgaaga
    26641 cgatgacgaa gctgtattcg agcttcactg ggataatcag caactagcag aattagtaga
    26701 agtaaaacag caaaatgaag cagatactta tttaccgtac ccaagttatt aaggagtgac
    26761 agatatgaaa tggaatgaag taaaagtaga aactgaaagt gaagcagtcg aagcagttgc
    26821 taatattttg atggaggcag gggcgagtgg tgtcgcgatt gaagattctt tagatgttga
    26881 acattttcag tcggatcaat atggtgagat tttagataaa aatgatttta catcgctgaa
    26941 agaaggcgca ctagtcatgg cttattttcc ggaaacgact ttcttaccgg aaatcttgcc
    27001 ttttatcaaa gatcgtatca cacaattacc tgagtttggt ctagctattg gaaaaaataa
    27061 agtaacagtt agtgaagtag aggaaagcaa ctgggcaaca gcctggaaga aatactatca
    27121 tccggtgcgt attacgcgaa tgctgacggt tgcgccaagc tgggaaaact accagacaga
    27181 agatcctcta gaaaaaatta tcacactaga tcctggtatg gcatttggca caggcactca
    27241 tcccacgact tccttgacat tgacagcact agaaacagtc ttgcgtggtg gtgaaacatt
    27301 gctagatgtg ggaactgggt cgggcgtgct aagtattgca gcaaaatatc ttggtgcaaa
    27361 agatgtctat gcctttgatt tagacgaagt agccgttcgt tcggcgaaag aaaatatgga
    27421 tatgaatgaa atagccaaag atgtccatgt atcagctaac gatcttttga caggaatcga
    27481 gattgaaagt gatgtaatcg ttgcgaacat tttagcagat atcatcttat tgatgatccc
    27541 agatgcttgg cgtttactga agcaaacagg cacgctgatc gtttcaggaa tcattgaaga
    27601 gaagaagcaa atggttctgg atgccatgtt agaacaaggg tttatcgtgg atcaaatttt
    27661 ccagcaaaaa gactggtatg caattatttt gaaaaaacca gaggtggaat agatgcagcg
    27721 gtatttttta aaagaatcat atgaatcaaa agactatttt gttttagaag gggaaaacta
    27781 tcaccacatt gtacgggtta tgaggatgga acccgaggat gaagtttttt tggcattcaa
    27841 tgatcaaata gccattgttg cagaaatcac ggagattact gaagaggctg tttatctgaa
    27901 agaaattaaa aaagaagaaa atcaaaagga actgccaagt tcgattacca tagcgagtgg
    27961 ctatcctaaa ggggaaaagc tggattggat cgttcagaaa tccactgaat taggagcgca
    28021 agcttttctt ggtttcccgg catcagcctc tgtcgttaaa tgggatgcaa aaaaacgtat
    28081 gaaacgacaa caacggcttg aaaagattgc tcaagaggca gcggaacaat cccatcggca
    28141 gatcgtgcca tcagtagagt tattggaaac gaaacaagat ttatttgagc ggctagatga
    28201 ttatgacgtg gtcttggttg cttatgagga atcagcaaag caaggcgaaa aatcaaatgc
    28261 agccaaaatt cttagtgaaa caaaaccaga tagtcgaatc ttagctgtct ttggaccaga
    28321 aggtggatta tcccgcgatg aagtcgatta ttttgtagaa cacaaaggga tactttgtgg
    28381 attaggacca agaatccttc gtacagaaac agcacctctt tatttattaa gtgctgctag
    28441 ctatcaatgg gaattaatga atccttcttt cagctaacgt tcttcattaa taaaagagta
    28501 tcgacagaaa gaattgaaaa aatagtcaac aatttgtata atagaaacaa ttatttatac
    28561 aatggatacg aaaaatcgtt tgaaaaagct tttaaaaagc gaacatttcc gtaaaaaaac
    28621 gtataactgc acggattttg ggacaattat caaacagata tttgtcccga ttcccgtttt
    28681 accgaaaaaa gaaaggggga tcgattcctg tgacaaaaga agaaatttta acgggacccg
    28741 gtgtcataaa gctcgttagt caatacatgg gtcccgaaca tgtagcattt gttgagaaag
    28801 catgcgaata cgcgactgct gcacatgacg gacagtttag aaaatcaggc gaaccctaca
    28861 ttattcatcc tatccaagtc gcaggtatat tagcagattt aaaaatggat ccccatacag
    28921 tggctacagg cttcttacat gatgttgttg aagatacaga aatcacttta gaagatctga
    28981 gagaagaatt tggcgatgac gttgctatgt tagtggacgg cgtaaccaaa ttagggaaaa
    29041 taaaatataa atcccacgaa gagcagctag cagaaaacca ccgaaagatg ctgctagcaa
    29101 tggctcaaga tttacgagtt atcatggtca aattagctga tagactacat aacatgcgta
    29161 cgttgaagca cctgcgagaa gataagcaaa gaaggatcgc tcaggaaact ttagaaatct
    29221 atgcgcctct tgcacatcgt ctagggatca gccggatcaa atgggaatta gaagatacag
    29281 cacttcgtta tctaaatcca aaacagtatt accgtatcgt ccacttgatg cagacgaaga
    29341 gagaagaacg tgaaaaatac gtaagtggta ctgttgaaga tattcgaata gcgacagaag
    29401 agctggggat ttttgcagaa atctatggac ggccaaaaca catttattca atctatcgta
    29461 aaatgaaaga tcagaaaaag cagttcaacg aaatttatga cttgctagct attcgagtga
    29521 tcgtagattc aatcaaggat tgttatgctg tattaggagc aatccacaca aaatggaaac
    29581 caatgcccgg cagatttaag gattatattg ccatgccaaa agctaatatg taccaatctt
    29641 tgcatactac cgtgatcggt ccggcaggaa atccggtaga aattcaaatc cgaacacagg
    29701 aaatgcatga aatcgctgaa ttcggggttg ctgcacactg ggcctataaa gaaggaaaaa
    29761 atgaaaaagt agaaccagat ggtatgacga aacaattaag ctggttccat gagatactcg
    29821 aacttcaaga cgaaagctat gatgcttctg aatttatgga aggcgtaaaa ggagatatct
    29881 ttagtgataa agtctacgtc ttcacaccaa aaggagacgt tactgagtta ccaaaaggat
    29941 ccggaccatt ggactttgca tacagtatcc atacagatat cggtaacaaa accactggtg
    30001 caaaagtaaa tggcaaaatg gtgcagcttg attacaaatt gaaaaacgga gatatcattg
    30061 agatcatgac ttctccaaat tcatttggcc caagtcgcga ctggttgaaa ttagttgcta
    30121 ctagcaaagc aagaaataag atcaaacgtt tcttcaaagc ccaagatcga gaagaaaatg
    30181 tgatcaaagg ccacgaatcc gtggtcaaat gtattacaga tctaggattt acgcctaaag
    30241 atattttgac gaagaacaaa ctgcaagaag cactcgatcg ttttaattat caaacagaag
    30301 atgatctcta tgcagctgta gggtatggag aagttagccc cttgacgatg gccaatcgtc
    30361 tgactgaaaa agaacgtaaa gaacaaaaaa tcgagcagca aaagcaagaa gcagaagaaa
    30421 tcatgaatca gccgaaaaaa gaacctgaca aaatgaaagt acgtcatgaa ggtggcgttg
    30481 tcattcaagg ggtagaaaac ttactaattc gtatcagtcg ctgctgtaat ccgattcctg
    30541 gtgatgatat cgttggttat atcactaaag gcagagggat atccattcat cgtcgagatt
    30601 gtccgaatgt tcagcctgac aaaccaaatg tagcagaacg tttgattgaa gtcgaatggg
    30661 aagatacatc gaatacacga aaagagtatg atgcagattt ggaaatttac ggctataatc
    30721 gttcaggctt attgaatgat gtacttcaaa cagtcaatgc gctaacgaaa aatctcaaca
    30781 gcgttgaagc acggacgaat aaagataaaa tggcgacgat ccatttgacg gttggtatcc
    30841 agaatttatc ccacctaaag agtatcgtgg ataaaatcaa agcagtacct gatgtctaca
    30901 gtgtacgccg gacgaatgga taggaggaca gatatgagag cagttgttca acgggtaagt
    30961 aaagctgctg tgtcagtcga tcagcaagaa attggaaaaa ttggctacgg cctacttgtt
    31021 ttattaggca ttcatgaaac cgatacgcaa aaagatgtgg actatctggt aaaaaaaatt
    31081 gcacaaatgc gtatttttga agatgaacaa ggaaaaatga atattagtgt agaagatgtg
    31141 ggaggacaaa ttttaagtat ctctcaattc acactgtttg cggatacgaa aaaaggaaat
    31201 cgaccaagct ttgtggaagc cgcacgacca gaaacagcta ttcctttata cgaatcattc
    31261 aatgaaggaa tcagaagtcg cggcatcaca gtcgctaccg gacaattcgg agcagatatg
    31321 tcggtttctc tcgtcaatga tggacctgta acgatcatta ttgattctaa aaacaaataa
    31381 agtaatctgg ttttcactag taaaaactat gaaacacgtc gcttaagtta gactgataaa
    31441 ctctaactta agtggctttt ttgtctgcaa aaagcgcttc ttttgtcagc agtcggaaaa
    31501 aaagttaagc ttaaattata caagttattt tttataggag gttgctgaag atgaaagaag
    31561 taccagtagt agatatcaaa atcacagatt taaaaaaatt gtatcaaaaa ggatacaaca
    31621 tgttggagga gttacgccat gaagcggacg ctcccgtcgt caaagcaaag attttcaata
    31681 aagaagctat cacgatctac ggatcatcag cagcaaaagt attttatgat ccaaggaatt
    31741 tcaaacgaaa aggtgctatg ccaaaattag tattgaagac actttttggt caaggtggag
    31801 tacagacttt ggatggagct gctcatcacc atcggaaaaa tatttttatg gatctaatga
    31861 caccggaaag aatggaagat tatcatcgta tcttagataa gaatctgaca caagcacttg
    31921 aagcacagca tggtcaattt gaactatttg atttaagtaa gatggttttc tttacatcta
    31981 tttgcgagtg ggcaggtatc aatttatcag cgatttctaa agatgaggtc gaaaaattag
    32041 cagaatatca aatctctatg atcagcggaa cgtttacttc tccgattgat cacataaaag
    32101 gtgtcgaaaa tcgcaaaaaa tcagaaaaat gggcgcaagg cttgattgaa gaagctcgac
    32161 aaaatcctgt ggctggaaaa gaaaatgtgg cattatatgc ttttgccaat gccacagatt
    32221 tagatggtca gcttttacct ctggaagtgg cagctgttga attattgaac atcatccgtc
    32281 caacggttgc tctaactgtg tgggcggcat tgatgggaca tgcacttttc agtagaccag
    32341 atttgtatca gcaattaaaa aatgactttt cgacgttaca agatccgttt attcaagaaa
    32401 tgagacgcta ttatcctttc ttcccaatgc tacctgcgat ttcgctgaaa gaagtagaag
    32461 tagatggtta tcgtatacca gaaggcagtt gggttatttt agatttatat ggaacagacc
    32521 atgatgaacg aacagttgaa gcgccagatt cctttatgat caaacggtat gtagggaaag
    32581 caaaagatat ttcctataaa gaagaatatg agatgattgc acaaggcggg ggaaattttc
    32641 gacaaatgca tcgctgtgca ggagaatgga tcactcttca tagtctacgt gtgttttccg
    32701 atcagctagt aaataagttt gagttctctg tacctgaaca agattggacg attccattca
    32761 atcagtttcc gacttatccg aatagccgag cgctacttta caagaattag attagcataa
    32821 acttatcgaa aaaaaggaag atcaaacagt gctgatcttc cttttttcat attactataa
    32881 attgataagg tcaattgatc aaaatccacg aaatgcttca tcaatggttt gataatcttc
    32941 attggataaa tgcaaatcta aagcttggac attgtcagat acttgctctg gacgcttagc
    33001 tccaggtata actacagaca ctcgtggatt cttcatgtac caagctaaaa caagttgcgc
    33061 aggagtggct tggtatcttt tagcaatggg gcgaagtaca tcgacagcgg ttaaaatttc
    33121 tctgaaacgt tctccttgga aatctggatt tctttttcga ggatctcctt caccaaattg
    33181 tttttcctca ccaagttcat attttcctgt cagcaaaccg gaagcaagag gaaaataggg
    33241 aacaaaactg attttgtttt tttcaagata agggaataat tctttttcag cctgccgatg
    33301 gatcaaactg tatttatctt ctactacatc gacatatcca tctgcattcg cttctttcaa
    33361 ttgttctaac gtgaagtttg acacaccgac tgctcggatt tttcctgctt cttttaattc
    33421 gtgcaatgta gcaactgatt cattttttgg tgtgctttca tccggaaagt ggatataaaa
    33481 aatgtcgatg taatcagttt gcagacgttt tagtgcatct tccaccgatt gtttgagaaa
    33541 ctcaggagaa ttatcaaatg ttcgcccttt atttggtaca tgagcagctt ttgtagcgat
    33601 gacgacacgt gagcgatcat attctttttc cttcagtacc tcgccaatca gttcttcgga
    33661 acggccgttt ccgtaagcat aagcggtatc gatcaaattg atcccgctat tcaaagctgt
    33721 acgaactact tgttttcctg tctcatcttc caaaccgtca aaaagattat gtcctccaac
    33781 ggcatttgct cctaatccaa gctgttctgc gtagacttgt gaatgtccga ttcttatttc
    33841 tgccatagct gattcctcct ttttgtctag tctaaaacaa aataccaaaa agctgaacta
    33901 aaacgttttt gtgaggggaa atagtcagat atctcttgac tttgagactg gtttttagta
    33961 ggttataagt aatatcgaat gtcaatgaaa gagcgagtaa gtttttgaaa ctttacaaga
    34021 gaggatcatc gaggctgaaa atgatctaaa gtttcattag cctaaagaca ctctggagat
    34081 ggacgagtga aaagccgtcc cgcgtacgag cgttattcgt aagaggaaag agaaatcttt
    34141 caaaattagg tggtaccgtg tatgaatttc attcaccctt gttcatgatg aacaagggtg
    34201 atttttttat ttacatagct aagaaaggga gagaaaacta tgagtttcca aagaccaaaa
    34261 ggaacaaatg atattttgcc aggcgaatca gaaaaatggc agtttgtcga agaaacagca
    34321 cgtttattat tcagagatta tcagtataat gaaatgcgta caccgatttt tgaacactat
    34381 gaagtgatat cccgaagtgt aggggataca acggatatcg tttcaaaaga gatgtatgat
    34441 ttttatgaca aaggggaacg ccatgtgacg cttcgaccag aaggtacagc accaatcgtt
    34501 cgttcttttg ttgagcataa attgttcggc ccagagtatg cgaaaccgtt caaaactttt
    34561 tatatgggac caatgtttcg ctacgaacgc cctcaaaaag gacggttacg tcaattccat
    34621 caaatcggag tagaagcatt tggatcagaa aaccctgcaa ctgatgtgga gagcatggtg
    34681 atggcacttg atttcttcaa acagttgggg atcacgcaaa tccggctagt catcaattca
    34741 ttaggtgata aagaaacgcg tcaagcctat cgtcaagcat tgatcgacta tcttttacct
    34801 tttgaatcag agctgagtga agactctaaa cgccgccttc atgaaaatcc attacgcgta
    34861 ttggacagta aagacaaacg agatcaaaaa tttgtggcag aagctccatc tatcttagat
    34921 tatctaagcg agcctgctca aaaacatttt gataccgttg tttccatgct ggatgcattg
    34981 gctgttcctt atgaaatcga cagtaatatg gttcgcggac tagattatta cacacatacg
    35041 atttttgaaa tcatgagtga cgcaccaaaa atgggggcac aagcaacgat ttgcgcaggc
    35101 ggacgttatg acaatctagt ggaagaacta ggtggtcctt caacacctgg cttcggattt
    35161 gcaatgggta tcgagcgatt actcttgact atggaggcag aggaagtagt tatccctgca
    35221 tttaatgagt tggatgctta cgtggtcgca ctcggagacg aaacaaatat cgaagcattg
    35281 aaagtggtcc aagcgattcg aaactttggt ttttcagctg accgcgactt catgaaccgt
    35341 aaagcaaaag cgcaatttaa gacagcagat aaagcgaatg ccaaattagt attggtgatc
    35401 ggtgaagatg agcttgctag tcaaacgatt aatgtcaaag caatggcaag ccgaaaagaa
    35461 aaaagttttc cattagaaga gatatatgaa cattttgatg aagtctatga tgaaatgact
    35521 ttattcgagt aggaggaaaa aaataaatgg cacaaagaac gatctattgc ggattagttt
    35581 ccgaacaaca attagaacaa acagttacat taaaaggatg ggtacaaaaa agacgagacc
    35641 ttgggggagt aatctttatc gacctacgtg atcgtgaagg aatcgttcaa atcgtcttta
    35701 atcctgaaat caacaaagaa gcatgggaaa tcgcggataa atgtcgcagt gaatacgtga
    35761 tcgaagtgac tggggaagtt agaagacgcg gagaaaatgc agtaaatcct aaaatggcca
    35821 ctggtgaatt cgaagtgatt gcaagtgaaa tcgtgatttt gaatcaagca aaaaccccgc
    35881 catttcaaat tgaagatgat caagcaatca gtgacgaaat acgtttgaaa tatcgttatt
    35941 tggatctgcg tcgtccaaaa atgactaaaa acatcaaatt aagaaatgcg acaacaaaag
    36001 cgattcgtca ttatttagat gataatgact ttctcgatat cgaaacacct tacctaggga
    36061 aatctacacc agaaggcgca agagactacc tagttccttc acgtgtccat gctggacatt
    36121 tctatgcgtt gccgcaatct cctcaattgt ttaaacaatt gctgatgaat gctggattcg
    36181 accgttatta tcaaatcgtt cgttgtttcc gcgatgaaga tttacgtgga gaccgtcagc
    36241 ctgaatttac gcaagtagat attgaaacaa cgttcttaac agcagaagaa atccaaactt
    36301 acacagaggg attgattgcg aaggtaatga aagaagttcg cggtatcgaa gtcacattgc
    36361 catttcctcg tatgacctat gatgaagcaa tggctcgcta cggcagcgac aaaccggata
    36421 cacgttttga tatggaattg atcgatctaa gcgatacagt caaagaagta gaattcaaag
    36481 tcttccaaat ggctcttgaa aacggcggtg tcgtgaaagc attgaatgct aaaggcgctg
    36541 cagatcgcta ctcacgaaaa gacatggatc agttaggtca atatgtagga caattcggag
    36601 ctaaggggct tgcatggctg aaagtagaag aagacggatt gaaaggtcca attgctaaat
    36661 tcatgggaga agcaacagaa gcaatcatta aagcaacaga tgcaaaacca ggtgacttgc
    36721 tgatgtttgg cgcagataaa agtgaaatcg tcgctgctgc attaggtgct atccggacac
    36781 gtttaggaaa agaattagga ctaattgatg aatcgaaatt caatttctta tgggtaactg
    36841 attggccaca atttgaattt tcagaagaag aaggtcgata tgtctctgct caccatccat
    36901 tcacgatgcc aaaagaagaa gatatcccgt tattggctga agaccctgca aaagtctatg
    36961 cagaagctta tgatatcgtc ttgaacggtt atgaactagg cggaggttct atccgtattc
    37021 atactcgtga gcttcaagaa aaagtactag aaactctagg ctttacaaaa gaacaaatgg
    37081 aagaacaatt tggtttcttg ttaacagctt tagattacgg attccctcca catggcggta
    37141 ttgcattagg attggaccgt tttgcaatgt tattagccgg agaagataat attcgtgaag
    37201 tcattgcctt tcctaaaaac ggaaaagcag cagatgtgat gagtgatgca ccaagtatgg
    37261 tttcgccttt acaattgttc gaattgaata ttgatgtaac gaatatagaa gaatagccga
    37321 taaaaaggta aaaaggattt gtcgaatacg cttcttttga caaccttgat ttaattgaaa
    37381 aacactttat aatgaagcca taacatataa agcggggctt ttccatgaaa aaaacattcg
    37441 aaaattgggc ttatcatgat tacacgacca agctttcggt atctatcgtt tatgcgttga
    37501 tcgcatcagt cgcattgaat ttcttttatc agccaggaca tatttattca agtggtatta
    37561 ctggtctagc tcagattttg acgactttgt caaaatcact gataggattc gaagtaccgg
    37621 tatcaacaac tttgtattta ctgaatatac ctttgtttgt attggcgtgg ttaaaaatcg
    37681 ggaagaagtt tacaattttc acttttatca cagtcacttt gacctccctt tgtatccata
    37741 ttttaccgga aacagtccta tctaccgatc caatcatctg tgcgttgttt gggggggctg
    37801 tgatgggact aggtattggg ctagcgttga gaaacggcct atcatcgggc ggtctagata
    37861 tcgtgagtat cacgattcgt aaaaaaacag gaaagtcgat tggttctatt tcgatctact
    37921 tcaatgcatt gatcgttttt gttgccggaa ttctatttgg ctggcaatat atgttttaca
    37981 gtacgttgtc tattttcgtc agtggaaaag taactgatgt cgtttatact aaacaaaaga
    38041 agatgcaggt aatgatcgta acgaaacatc cagaaaaagt agtaaatgaa atccaaaact
    38101 gtatgcgtag agggatcacg atcatcaatg aagcagaagg tgcttataag catgataagc
    38161 agacagtatt gatgacgatc gttacacgat acgaactgcc tctacttaga gaagcaatga
    38221 aagcttctga tccacaagct tttgtaagta ttgcagaaaa cgtccagata cttggtcgtt
    38281 tctatgagga tggcatttag atcgagtttg acaaaaattc atgaaatact gctgtagaca
    38341 gacgttcgtt tgtctgcagt ttttttctac catagtagaa tcagcaaaga aaaactgctt
    38401 ttttatttga aattttctta aagttgaaaa aaagcatgtt tattttttta aatttatggg
    38461 atgatagaaa aaaatagtag agaaaggaca cgattctact tgctattttc acttaatacc
    38521 cgtatattac gaatgaaaaa agtttatgaa aagttggaat attgaatgga aaaacaaaaa
    38581 aggttaaaaa acagccgcta catcactggg tttgatgggc tccgttcatt agctgtggtg
    38641 ggtgttatct tctatcacct tctgccaact agtatgaaag gcggctattt gggcgttccg
    38701 atttttttcg tcgtatctgg ttatttgatc acggatctgc tgcggcagga atgggaacaa
    38761 aatggaaaaa tcaatatttg gcaattttat gtcagaagaa tgaagagact ctatccggca
    38821 cttgttttta tgcttgtgac agcttcggct tatatcactt tgtttcagcg agggctgtta
    38881 aacaatcttc gaggagttgt ggtcagcagc ttactgtatg tcaacaactg gtggcaaatc
    38941 aataacggtt tatcttattt cgatcgattt gctaatgagt caccgtttac gcatatctgg
    39001 tctttagcag tagaaggtca aaactatttg gtttggccga tcttgtttgt attgttgatg
    39061 gtatttgttc gtaaaagaaa atggatcttt tatactgtgt taggtacatc cttcctctcc
    39121 gccatcttga tgatgatctt gtatacacca ggaggcgatc caacccgtgt ttattacggt
    39181 accgacactc gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt ttggccaagt
    39241 acgcgtttaa agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat cgctggagga
    39301 atctcgcttt tggcactgat tctttgcttc ttctttttag atgatcatta caatttcatc
    39361 tattacggag gaatgctcct agtgagtctg ttatgcgtag tgctagttgc agtgacagca
    39421 caccccggag caagtttgaa tcgatggctg acaaatccag tatttacttg gattggcaaa
    39481 cgaagctatg gcatttatct gtatcagttt ccagttatga ttttttatga agcaaaggtg
    39541 actgatattg ccgatcatgt tttgttgcat actttagtgg aaatcacatt gattctaatc
    39601 atcagtgaac tttcttatcg atatgtggag cgtccgctag cacgttttga ttatggtcgt
    39661 acattccaag tgatgaaagg atggttcagc aaaccgatca tcagtaagag caaaccatgg
    39721 gttgttccag gcacattact tgtagcaatt gcactcgttg gtttcgttac ggcaccgaaa
    39781 aacgctgtaa cagccgatca aaaacaactg caggcaaaaa tcgctgaaag taaaaaatta
    39841 gcagaagaaa ccagaaacaa ttcagaaaac accactagtt cggttgacca agcgatcctt
    39901 gataagtacg agttgtccaa ggcacaaggg aataaggcac aaaaattaga actaacggca
    39961 tttggtgatt cagttatgtt ggatgctgca acggatctac aagaacttta tccaaaagta
    40021 gtggtagatg gagatgtcgg acgacaatta tatgccagtg taccgtacat tgaaaaatta
    40081 aaggaacaag atttgttgaa agacactgtt ctgatcggac taggaacgaa tggctcattc
    40141 tcggaagctc agtttgatag tgtgatggat gcaatcggtg atcgaaaagt atactgggtc
    40201 aatgtccgag taccgacgaa gcgctggcaa aatgacgtga atgcgatgct tgaacagatg
    40261 acgaaaaaat acgacaatat gacgctgatt gattggtaca gctacagtaa tgaccatagt
    40321 gactggtttt atgatgatca agtccatcca aatcctgaag ggatgacaca ttacattcac
    40381 ttagtcagcg aagccttgct aggaggaagt tcaagtacga acagcagtga gacaaacgtg
    40441 acagaatgat agctacaaaa aacttcaggc aaaaacattt gtttttgcct gaagtttttt
    40501 tatatttacc gtgtttttaa cattcatata gattcaggaa gcaaaacggt tgagtgtgtc
    40561 tgtaaataac gttctttttg ttttcgggtg agttttttga atgcagcttc tgcttttgtt
    40621 gcttcgcttc ttgaagaaaa tacttctgca tggatcatct taacaggtct tcttttttga
    40681 agacgagtat atttagcacc agtaccattg ttatgttcct ctaatcgtct agaaagatcg
    40741 gttgtatatc caccataaaa tgtctgatct ttacagaata atacgtaaaa ataatgttca
    40801 tttgccatag agcatctcac gaatctctgc agtgtattct ccatccatat tgtaagtgat
    40861 caatggtgga gcgactttga atccgtctaa tttgccttct ttgattcctt caactaacaa
    40921 aatatttgcc tcttttccag gttttgggta aacaaattgg atccttttag gtgcaattcg
    40981 atagtcttcc atgacctgaa tgatttctaa gaaccgatca ggacgatgga ccatagtaaa
    41041 gcgtccattt gtttttagta gcttacttga aatatcgatc acttcttcta acgtcgtatg
    41101 aatctcatga cgggcgattg ccaggtgttt attagggttc gttttatttg ttggcagccc
    41161 tttgaaataa ggtggattac agacgactaa gtcacaagaa ttatgtcgaa cgacagataa
    41221 gctgtctttc agatcgatgg tgtggacagt catttgttca tttaaatggt taagcgcgat
    41281 actacgtctt gccatgtctg ctagacgagg ttgaagttcg atagcgtcga tagccgcctg
    41341 ggttcgttta cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt caacaatctt
    41401 ccctttttta ggtacacggg aaaagtgagc aagcagcaca gcatctaaag aaaaagaaaa
    41461 cactttggaa ctttggataa tcttgatatc ttctgcgtat aattggtcga ttcgttcgtc
    41521 ttcgtgaagc atggattccc tccaaaatga aaaaaatagt cgtctgtatt atactatttt
    41581 tattaaaaag aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac tatgaccata
    41641 agaagggaaa ggaagagact atgttttatc gatttatgcg cggggtagtt cgagtactat
    41701 tagccttagt taatggaaat gcccgctatg agaataaaga agttttaccg caagatgaga
    41761 attacatact agttgctccc catcgtactt ggtgggaccc attatatctg gcggtagctg
    41821 cagcacccaa ggaattcagc tttatggcaa aagaagaact gttcaaaaat ccagttctgc
    41881 gatttatatt aactcgatcc aatgcttttc cagttaaaag agaaaatcca ggaccaagcg
    41941 tcattaaaac acctgtaaag attttgaaat cgacaaattt gagtttgatc atgtttccca
    42001 gtgggacaag acatgctaca gaacttaaag gcggaatggc attgatttcc agaatgtcta
    42061 aagcgaaaat cgtgccagct gtatatcaag gtccgttgac acttggggat ctgttcaagc
    42121 gtcagagagt gacagtgcgt ttcggcgaac caatcgactt atcagatatc aagaaaacgg
    42181 ataaagaagg tatggcggag atcgaacgac gaacacaagc agcttttgac aaattggaca
    42241 aagaagtgaa ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat aagtttatgc
    42301 gtgggcggtc ttttgaatct actgtcctgt tcagttcaga ctttttcaat gaaaacgagg
    42361 gagagacgtc aagtctgccc tctttttgcg ttccaatatg taaaatagaa gacaggtgtc
    42421 atgtaatgga tcaaagtgtt ataataatta gaaggacatt ttttgaggtg attgaatgcg
    42481 ttttttacat acagccgatt ggcatatcgg aaaaaaacta caaggttatg acttgattga
    42541 agaccagcgt catgtactat cttctatact agatatcgcg aaagaagaaa aagtagaggc
    42601 aatcgtgatt gccggagact tatatgatag gagtgtccca gctgttgatg ccgtagaatt
    42661 atacaatgaa ttgatggtcg attggaattt aaaggaaaag atcccgattt ttgctatatc
    42721 aggaaatcac gatagcagta cgagactttc agcgggtacg ccatggttca gttattcgaa
    42781 ctattttttg cgaacacagc tagcagaagc atttgaacca gtcgagttta aaaacgtaca
    42841 atttttcttt cttccttact ttgaaccaat tgcagcgaga ttatattttg aagatgacaa
    42901 tatccgaacg atcaagcaag cggtagccaa ggtgatagat aaaatgcagg agctatttga
    42961 ttcagaaaaa aaacaagtat tgattagcca tttttttgta atgggaagtg taaaatctga
    43021 ttcggaaacg aaaatcgaag taggcgggtt ggattcgatc cctggtgaac tacttacacc
    43081 gtttgattat gtagcactag gacatttgca taatcaagct gctttgagac aggataatgc
    43141 tcgctatagc ggttccccat tgaaattctc tttatctgag atcaaccaaa aaaaaggcgt
    43201 atggatcgtt gacctgacaa caggcctgga gttgaatttt agggagatcg aacccctgca
    43261 tgatattgaa gaaataacag cgagtttcaa agaattgctg gatcctgcct tttatcagtc
    43321 cgtcaatcgg gagaattaca tctatatcta tctcactgat agaacgatca tcccgaatat
    43381 gatgaaccag cttcggcaaa tatatcctcg tattttgggg attgaacggg caaacggtcg
    43441 agcagaacaa acaaagcgca aacgaaaagt actagaaatg aaagatcctc aaaaattagc
    43501 agaagcattt tttagtgaaa tgacggaaga gcagctaact gcaaaccaag aaaaatggct
    43561 cggagaaacg ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac cgaaaaaatt
    43621 acaacttaaa aattttggtc cttttatcaa tgagaccgta gatttttcac gtttgacaga
    43681 agcaccgctt tttttgatca gcgggaagac aggagctggc aaaacgacga tttttgatgg
    43741 gatcaccttc gcactttttg gtgaaacttc cggaagactt cgctctggaa aagagatgcg
    43801 ttctttgttt gctacaccag acgaagaaac aagcgtgact ttttcttttg aacatcagca
    43861 gatgagctat gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa aaggaaacgg
    43921 aacaaaaaaa caggcagcca aagtaacgtt gaccattttt gatgggaaag gaaaagaaat
    43981 aaaacaaatc acaaaacgta cagaagcaga tcagttgatc agagaactga tgaatctcga
    44041 tgcgaaacag ttttctcaaa tcgttttact accccaagga gaattccgta attttttggt
    44101 ttcatcaagc agtgataaag agactgtttt acgtcattta ttcggtactc aatttttcca
    44161 acagttcaat gagcgattaa aagaaaaagc aaaacaacag caacaaaaat tggatcattt
    44221 agagcaagag ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag aggaaccagc
    44281 agcagggttt gaagccgttt tagcccaatg gacaaaccat caggcaatac tcgaaaaaca
    44341 aatcacggaa gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc aactagaaaa
    44401 ttcctactat cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga tattaataaa
    44461 caagcaaaag gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac gatggattgg
    44521 gtattatgaa tttacgcaac gattagtcga accaatccag cgcctacaag aaataaaagt
    44581 tgaaaaaata gaactgtcac aaaaaagtag tgagcaaaca aagaaactag cgatgactac
    44641 gcagaattat gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac aaacaaaaat
    44701 caaagaagct gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg tcgaatcctt
    44761 acgtagtaaa aaacaagacg cagaacaatt aacaatttca ttggataaag aagaattgaa
    44821 tgccgagaag ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa ttcttcaaga
    44881 aaatttactg gatcaggaaa aactgcaaga agatcgtctt gcttttggac agttaaaata
    44941 tctagatgaa aagcagcaag aaatagaaaa acaaaaagca aaagacactg ggtcgctcgc
    45001 acagataaga gaagaactca aaaaagcaac tgaagaaata acaaaaaata gtcagctatt
    45061 gaaagaacag acagaacaag cagaacagct gaaaagccaa tgggcaaagc agcaaattgc
    45121 tcgtcttcag ctttttttgc gtccagggga gccatgtctt gtttgtggat caacggaaca
    45181 ccccatacaa caattaaaac acgaacagcc aaataaggaa gatatcttaa aagtagaaca
    45241 acaactggca gaagctgagt ctgctgtaga aagaactcag caaatcattg ccaaaagtga
    45301 agcacatgaa aataatcttc aacaacaatt atcagattta gaacaaaaaa tcatggcaac
    45361 gaatatacaa ttggctgaac aagaagaaca cttgcaacag gctttgaaaa aggcatttcc
    45421 tgagataaca gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat ctttagatag
    45481 acgaaaaagc aagatggcca atgcaagaat agagctggaa gaaataagac aacaagcaga
    45541 aaagctcgaa caacaattag ctgtctacaa acagcagcag acagaaaaaa ataatcagct
    45601 ccagcaactg caaggagaaa tcaaaagttt agaacaacaa ttgcaagata cggatagtaa
    45661 ctgtttagaa gaggagatac gacgacttga aaaacagtta gaagaagact cgaatcaact
    45721 agctgttgag cagaaagcag gagtgcaatt gttgcaagat cttcatacgc ttcggacaca
    45781 gcttgatctt caacatgaac aaatagaaaa aatagatgaa aaaatagaaa aggaacaaga
    45841 aagaatagct aaaaagttag aaagccagtc gtatttttca acaattgaag aaatctgtgc
    45901 atttgcacaa cagcaagcac aatataaaga aaaccaacgt acagttactt cttatcatga
    45961 agagcaatta gtgacagctg accggctgcg tcaattagaa aatatccagc taactgagac
    46021 attcccagat acagaagaga tcagccaaca gttagaagaa ataagacgta tgatcgaaat
    46081 aaagcaagaa aagatatacg gtttacaaga acaaaaaatt agtaatcaaa aaatctacga
    46141 tgaatgtctt tcgatttatc acacaagcca gacgaaatta gatgagctta cgcaattaca
    46201 gcagctttcg caaacattca acggagaaaa tcctaagaag actagtttgg aacggtatgt
    46261 gcttcaggtt tatcttcaag aagtgttaca agtagcaaat gatcatctcc aacgtttaac
    46321 gaaaaatcgt tatcaatttg agctagcgga taccattggc agttatcgag ggaaaaccgg
    46381 attagaaatc aatatctatg atgatcaggc aggtatgtct cgaagtgcgc ataccctgtc
    46441 tggcggtgag agttttattg cagcactttc tttagcatta gctcttgccg atgtgatcca
    46501 gacacaagct ggtggtgtag caatcgatgc attgtttatt gatgaaggat ttgggtcatt
    46561 agatgaagaa gctttggaaa tggcaatgga agctttagaa acgatcgaaa gtgaaggaag
    46621 aatgatcggc atcattagtc atgtaagaga attgaaagaa cgagtattac aacaaataag
    46681 aattgataca gaaggtagcg gacaaagcaa aatacgctat caacttggat aaaaagggag
    46741 attggtaact atgaaatgga gtattccaga aaaagtcatt gcacgtggac gtcaatactt
    46801 gcaagaaggc cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt ggcatgcaga
    46861 agtcttgggc agtgaattgt atatggttga tttggatggt acagcaaaag agatagatta
    46921 ctgccaatgt ccctattggg aggaacataa atattgtaag catacagttg ctgtggaact
    46981 ttatttacgg tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa tgctagaacc
    47041 ggtaaaagca acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc taaaaacaca
    47101 aaacaatcct caagcggtgc aacctttgat tattgaatac caagtagaaa cgattgagac
    47161 taatcagtat cacccagagc tttctatttt ggcactttac ttgcgtatcg gttcattggc
    47221 atcaccgaaa aaaacatata tcgtaaaaaa catctatgat tttttacaag cttacacaga
    47281 aaaagaaact tacactgtta ataaacagta ccagtttcgt ctagataaac gggcgtttca
    47341 aaaagaagat caaaaaatac tcacgcatct tgcaggaagt gctcagacgc atcaattatt
    47401 aggtacgaac ggcttacaag tcaaaggaaa actagataaa agatatctgc ttctcccagt
    47461 agaacaagcc cgcttcctgt tagagcagat gaatcagact acacgtttgc atctgcagat
    47521 aaatgaacaa aacgatcaag gaatacaatt ttccgatcaa aacaaacctt tatcattcaa
    47581 agtggaaaaa tttggagaag attatttgct gcaagcattg aatgattttg attttttctt
    47641 tatgcattat cagtggggag ttatccaagg gacattttat tctctgacga aatatcaaca
    47701 aacggtttat caaacttgga aacaattgat tcgccgattg gagaaaccag aagtagtgtt
    47761 caagaaaaaa gaattaccgt ctttgtttaa agagattatc cctctgctgt cagagatcgg
    47821 cgacgtagct attgcccctg cagtagaaga ggaagtagca gatatccccg tcgaatttat
    47881 tttctttttc cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg tctatggcga
    47941 agtcatctat tcgactgacc cgaagcatga ggtctcgaca gaacaggctt ttcaggtgtt
    48001 acgtgacaca gtagaagaga aacgagtact tgatctattc agaatgtatc gctaccagaa
    48061 aaatgaaact ggttatgaac gtccattgcc aagcggggaa gaactatatg cctttttccg
    48121 cacagaacta tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa aattgcgaga
    48181 gttgtattta gatgcgcata aacatcaacc gaaaatcgaa gtaaccgaga ccggttcatg
    48241 gctcgatatt cgattcgatg tgacagggat agaggagcaa gaaatcgatc atgtcctgca
    48301 aagcttgctg agaaatgatg ctttttatac attagaaaat ggtcaggttc tttcttttga
    48361 ttcagaagaa tttcaacaaa caagccaagt gttgcaacaa ttacgggaat cgatacgtac
    48421 agaagaagga acgatccatg tacctaaaaa tcaaggattg atcattcaaa atcaattgga
    48481 aaaaagcaat gctactttta gtgaatcgtt ccaaacgatg gtacaagatc tcattcatcc
    48541 agaaagatac caggcacagc ttcccaaagg attgaatgca acgatgagag attatcaaaa
    48601 gcaaggtttt cgatggttga agatgttagg tcattatcag tttggcggta ttttagccga
    48661 tgaaatggga cttgggaaaa cactacagac aattgccttt ttactttcgg aaaaagaaga
    48721 acggaaaagc ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca actggcaggc
    48781 agaggtgaga aaattcgccc cttctttaag tatccaagta atcaatggga ataaaaaaga
    48841 acgtgaagaa ttactggcta aagacacaga tatccgagtg acttcttatg ccagcttaag
    48901 acaggatcta gctaattatc agtcgcaaaa aattgattat ttgattcttg atgaggcaca
    48961 aatggtcaaa aatagcagta cgaaaactgc acaagcattg agagaattag ctgtacctca
    49021 gcggtttgca ctgagtggga caccaatcga gaataattta gaagaattgt ggtcattgtt
    49081 tgcaacgatt atgccaggat ttttcccgac taaaacaaaa ttcagggaac ttgccgcaga
    49141 agaaattgcg caaatgattc gaccgtttat tttacggcga gataaacaaa ctgtgttgaa
    49201 agacttgccg gaaaaaacag agatgaatct atattctgca cttacagaag aacaaaaaac
    49261 tgtttattta gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg attctgaagc
    49321 attcaagaaa aaccggatcg ggatacttgc tggtctgaca agactgcgac aaatttgctg
    49381 cgatcctcgt ctgtttattg aagattacca aggcggttcc ggcaagctcg aacaagtaaa
    49441 agatctactg gtagctgcca aagagaacaa acgaagagtt ttgctgtttt ctcaatttac
    49501 agggatgctg actatcttgc aagaagagtt agccgagcta gggatctcga cattttattt
    49561 aagaggcagt acgaaaccac aagatcggtt aagtatggta gacgcattta atgctggcga
    49621 aaaagatgtt tttctgattt ccttaaaggc tgggggaact ggattgaatc tcacgggagc
    49681 agatacggtc atcttgtatg acctttggtg gaatcctgcc attgaagaac aagctgctgg
    49741 acgtgcacat cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga tctctgaagg
    49801 aacgattgag gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt tccaaaaagt
    49861 gatccaaggt aatgaagaac agttgacgaa actgactgaa gaagacatcc gcatgatttt
    49921 aagtgtagga gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat gggtaagaaa
    49981 gcctttctaa agaagcgatt gtttatttga tttaaaaaat agcaaggagg ctgggattcc
    50041 attttatccc agcttccttg ctatcatttt tttatttcta tttttaaagg ttatacaaag
    50101 attgtcttca ttgtctctaa cagctgatca aaagaagaaa tctcgatatc gcaaattgga
    50161 gtctgttcaa ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt agcacttttt
    50221 gcacctagaa catcattgtc gtaattatcg ccaacgtata aggtctcttc tggaagcata
    50281 tgaaattctt tctcagctag ctgaaaaatc tctttttctg gtttttggaa acctgttgct
    50341 tgagaaatga gcatattgcg ggaaggaatc caattgttca attgaagttg cttcagtttt
    50401 tttgtctgat ggtcagtcgg cccatttgtg ataattccta atgggacatt cttttccttc
    50461 aagaaatcga gggttttttt gacctcctca tgcaagcaga tattatcaag ttcttcctcg
    50521 taaatttttt gaaataagag gccatcttct tccgtaatat gagggtagtc taaatctttt
    50581 agtgattggc tgatccgatg tgctcgcata tattctagtg tccaatctcc tgccattact
    50641 tttgggaagt tttcatcact atgatggcgg aaccggatat atagtggatg catatctgca
    50701 tctgaaacaa gcggaacgac tcttttgacc gcgtttcgaa atggctgctg ctgatcatac
    50761 atcgtatcat caacatcaaa cacaactgat ttaatcaaaa ttttacacgc ctttcggttt
    50821 tcattgattt tttcacaaat ccttgactat tgtaacagaa aaaattgtct aaaaaagctg
    50881 ataaataaag gtttttctta ctatatttct atttccttga cagaataagg tttttatatt
    50941 tcagtttttt tcactaaaaa tcgtgaaaca cgtcaataag tttgatgaac aaattaatca
    51001 ttatggtaga agtcgattga tttattttaa tgcaatctct gtggaatttt cgaaagattt
    51061 atttttttaa tacagtctca atataactat tcaaacataa aataacgtta tttgatttat
    51121 ttgtttatag taaattttat ttagtaatca ttgctttaaa atactacgct tatataacag
    51181 agaaaataag cgttataata aagaacagta ttatttaaaa tgacagaaaa cattactatt
    51241 aaagaaataa aaaacataaa aaatcttaag aaatcggttt catttagctt ttgagataaa
    51301 ataatagtac agttttatac gctgtttcat atgtttagaa aatttgaatt agatttgaaa
    51361 actctaattt ttaaatgata aaaaaagcga gaatgctggt tttactgtat attaacctta
    51421 gataactttc atttttttaa aaaaattatg aaaaaaaggt agctatttaa aagttgatta
    51481 tgtataatag aaacagatgg gagattatta tgatcttctg ttacaaaaaa gtgattctta
    51541 tcacgaaaag gagtggtgca cgttgttaac attatatact tccccaagtt gtacttcctg
    51601 cagaaaggct cgtgcgtggt tgcaagaaca tcagattcca tttgttgaac gtaatatttt
    51661 ttcagaacca ttgaacagtt ctgaattaaa agcgatttta caaatgacag aagacggaac
    51721 agaagaaatc atctcgacac gttccaaagt attccaaaaa ctgaacatgg atttagatga
    51781 tctgccgtta caagaattat tggaacttgt gcaaaacaat cctggattgc ttcgccgtcc
    51841 aatcatgatt gatgataaac gtctgcaagt tggttttaat gaagatgaaa ttcgtcggtt
    51901 cctaccgcgc gatgtacgac aattagaatt acgtcaagca caattgatgg ctggattata
    51961 gaaggataat acgaaacttg gaaaagacta ttacaaagat agtttttcca agtttttttg
    52021 tttttacaaa aagtattctt gattagatgt ataaaaaggt agtagaagat gatgattgca
    52081 cgaaaaatag agagggaaaa ttagctgaaa agtcggctat atagtattcg tctgccaaaa
    52141 atgttatagt taaataagat agcttgaaag gttgaaaaat ccgctggttt tattaaattc
    52201 gcttaacagc gagatttttt ttctttacat ttaagctgat ttcgctacaa tgaatctata
    52261 ttaaacattg tggcaaagtg aggtgtagca aaatggaaat ggaacatatt aatgaaaata
    52321 cgatccgtgt actaattggt catgaagatt tagaggaacg gggcgtttca tttttggatt
    52381 tacttggcaa tcacaaagaa atcgaaaatt tcttttatag tattttggaa gaagttgata
    52441 tcgaagagga gttccgagga agcgaagctg tcactttcca agttttgcca aaaggagacg
    52501 gattagaact gtttatcagt aaaaatcttc cggatgaaga aatttctcaa tcagaagatt
    52561 ctactgttga ttcagaagat gtgagtgact ttcttcgtca gcagattatt ggcagcgatc
    52621 cggaggaaga attatctgga agcgccctag aagaacagga aagacaagca acttttgctt
    52681 ttgaagattt tgaacaagtg atccaacttg caacagatgt cgaactagag tctgcttgga
    52741 ctgatttgta tttattcgaa gagcgctatt acctagtcgt tcacttctgg atggaaaatc
    52801 tcaaccaagc ggatgtagag aatcagattg ctcgaatttt ggagtttgct gaaaagagcg
    52861 atcgtacacc agaaacattg aatgaatatg gacaatgtct gatggcgcgt aatgctatcg
    52921 agcgaacgaa attttatttt gtataaatca gcattatagt aagagactgt gacaaaaatt
    52981 gtgtcgcagt ctttttttcg tataaatgtg ttcaaaagct tgcatctgcg gtaacaagcc
    53041 caaccaaacg aaaaatattg ggaactattt ttgtttagct gttcttatta cttgatgcag
    53101 aatagctttt tcacgccctc ttttttcgtg taaataaatt cttagacgta aataagttca
    53161 gagggagaaa aatgctgctt gcaatgaatg aatataataa gtgctgtttt gctaaacatg
    53221 cagaaaaagg aaagaaatat tattgtcgtg gctgtcgaaa aagagtgatt ttgaaaaagg
    53281 gaaagaaaaa atgtgctcat tttgcccatc aaaaatcaga taactgttcg gtctttagtg
    53341 aaagagaatc aaaagagcac ttacagttaa aggaatgctt catggattgg ctgggacagt
    53401 cagtagaacc agtatttttg gaagcctatt tgccgaggtt gcggcaaagg ccggatattt
    53461 tgttggcgaa tctagcaata gaaatccaat gttctagact gtctcaccag agatttattg
    53521 aaagaaccca aaattatttg aataacagtt accaagtatg gtggatactt ggtaattctt
    53581 ttcttggaca atcgcaattc tcattgatag aaaaaagttg ctgctactat aatagaaaaa
    53641 gaggagtgca ttgctggaaa gctgatctca agaaacaaaa gctttatttg tatcatcata
    53701 ttacagagac tgtatcaggg catatttcct tttgcagtag ctgctggact ttttctagta
    53761 gggaccttaa agaaatattc actagtaacg aaattaaaat gaaccaaatg aaaaaaatag
    53821 aaaaagtgag tgaagatggg aagaaatggc tgacacgcca acttattcac aagcaaaaga
    53881 atacagtatc catacaagaa caatgctact tgcgtcataa acatcttttg catctatcat
    53941 cgtggatcta tcaaaaaagt cggtttttct tttatttaca agaacaggtt tttctttatc
    54001 gaatgttgta cgaagaagcc ttggaaaacc agaaagttcc tgatttcaac tcatggcttt
    54061 gccaaataaa agaacataaa agggaatggc tttttcctat gattgatgaa gagatggtct
    54121 atcagcagtt ttttaacgaa tgcatacact tgtcttcttt gaaaaaatag taagtataaa
    54181 aataggctag gaatcggttt tctatgtaaa acaagattta gttatgctaa aattgatgga
    54241 agcagacaca tgaatgagta gaaaagttag atgctgtgac atgtggatgt tagactatga
    54301 tctattttga tagatagagc taagggaaga aaaggagtgg atttcatgga agtaaagcag
    54361 ttgccaaaac gagaagaatt gcctgaaaat ttaacttggg acttgaccaa gatcttttca
    54421 agcgaccaag agtttgatga gaaatatttg gaattatcag aagagttaaa acaatctgaa
    54481 aaacacaaag gaacacttga tcaaggcgct tctcaatttt taaatgcgat tgaattcgta
    54541 ttgagggttt atcgccaaac tgaagtcatt tatgtatatg cgcaccttaa aaacgatcaa
    54601 gacactggaa atacagatta ccaagcgctt tatgcaagag caagcagtct gttttcgaaa
    54661 gttagtgaag ccgtttcctg gtttgaacca gaaatattgc aattgtcaga tgaccagatt
    54721 tggcaatatt tcaaagaaga accaaaattg gaagtctatc gccattatat ccagcaaata
    54781 gtagataatc gagcccatgt cttatctgct gagcaggaat ctcttcttgc tggagcaggt
    54841 gaaatctttg atgcttcaag tgatacattt gctgttttga ataatgcaga tctagttttt
    54901 ccaacgattg aaggagaaaa tggtgaaata gtccaattat ctcatggcgt gtatggtcag
    54961 ttgctagaaa gcacggatcg aagggtgcgc gaagcagcat ttaaggggtt gtacagtgtt
    55021 tacgaacaat ttagaaatac atttgcttct actttaggca cacatataaa aggacataat
    55081 tttaaagcga aagtccgtaa ttacagctct gccagagaag cgtctttgag caataatcat
    55141 attcctgaaa gtgtatacga tactttggta gacgtggtaa acaagcattt gcctttgtta
    55201 catcgataca tggaattacg gaaacgttta ttagaagtgg aaaaactgca catgtatgat
    55261 ctttatacac cggtcttagg ggaagctcca attaccttta cgtacgaaga agcaaaagaa
    55321 aaagctttag aagcactgaa accaatgggt gaagaataca tggccatcgt agaaaaagca
    55381 ttctctgaac gttggatcga tgttgtcgaa aataaaggga aacgaagcgg tgcttattct
    55441 tcgggaagct atgacacaaa tccatatatt ttattgaatt ggcatgatac gctggatcag
    55501 ctatttacgc ttgtccacga aatgggacat agtgttcata gttatttcac tcgttcgaac
    55561 cagccttatg tgtacggcga ctactccatc tttttagcag aaattgcttc gacaacgaat
    55621 gaaaatatcc taacggagta tttattggaa acagaaaaag accctcgtgt acgggcttat
    55681 gtactcaacc attacttgga tgggtttaaa ggaacagttt tccgccagac acaattcgct
    55741 gaattcgagc atttcatgca tacggaagat gaaaaaggtg tgccattgac tagtgaatac
    55801 ctaagtgata gttatggtaa attgaatgca aaatattatg gtccagcagt cgaagaagac
    55861 ccagaaatta aattcgaatg gtcacggata ccgcattttt attataatta ctatgttttc
    55921 caatactcga ctggcttttc tgctgcttca gcactggcga agaaaatact aaaccaggaa
    55981 ccagaggcac tagagaacta cttggcttac ctaaaagcag gtaacagcga ttatcctgtg
    56041 gaagtaatga aaaaagcagg agttgatatg acacaggctg catatattga agatgcaatg
    56101 tcgatgtttg aacaacgtct aaatgaatta gaagaattga tcgatcgttt a
//
LOCUS       DACGLH010000011        52830 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.012, whole genome shotgun sequence.
ACCESSION   DACGLH010000011 DACGLH010000000
VERSION     DACGLH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.012"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IXT21_000738"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IXT21_000738"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ4868166.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IXT21_000739"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IXT21_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ4868167.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IXT21_000740"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IXT21_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ4868168.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IXT21_000741"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IXT21_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ4868169.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IXT21_000742"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IXT21_000742"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ4868170.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IXT21_000743"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IXT21_000743"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ4868171.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IXT21_000744"
     CDS             complement(8053..8523)
                     /locus_tag="IXT21_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ4868172.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IXT21_000745"
     CDS             complement(8569..8994)
                     /locus_tag="IXT21_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ4868173.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IXT21_000746"
     CDS             complement(9061..9330)
                     /locus_tag="IXT21_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ4868174.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IXT21_000747"
     CDS             complement(9341..10936)
                     /locus_tag="IXT21_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4868175.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IXT21_000748"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IXT21_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ4868176.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IXT21_000749"
     CDS             complement(14489..15049)
                     /locus_tag="IXT21_000749"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ4868177.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IXT21_000750"
     CDS             15402..16376
                     /locus_tag="IXT21_000750"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ4868178.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IXT21_000751"
     CDS             16546..17946
                     /locus_tag="IXT21_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ4868179.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IXT21_000752"
     CDS             complement(18024..18746)
                     /locus_tag="IXT21_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4868180.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IXT21_000753"
     CDS             complement(18825..19331)
                     /locus_tag="IXT21_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ4868181.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IXT21_000754"
     CDS             19454..21253
                     /locus_tag="IXT21_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ4868182.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IXT21_000755"
     CDS             complement(21321..22895)
                     /locus_tag="IXT21_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868183.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IXT21_000756"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IXT21_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ4868184.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IXT21_000757"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IXT21_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ4868185.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IXT21_000758"
     CDS             complement(24672..25310)
                     /locus_tag="IXT21_000758"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ4868186.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IXT21_000759"
     CDS             complement(25311..25961)
                     /locus_tag="IXT21_000759"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ4868187.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IXT21_000760"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IXT21_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ4868188.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IXT21_000761"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IXT21_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ4868189.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IXT21_000762"
     CDS             complement(29104..29844)
                     /locus_tag="IXT21_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ4868190.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IXT21_000763"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IXT21_000763"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ4868191.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IXT21_000764"
     CDS             complement(31215..32153)
                     /locus_tag="IXT21_000764"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ4868192.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IXT21_000765"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IXT21_000765"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ4868193.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IXT21_000766"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IXT21_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ4868194.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IXT21_000767"
     CDS             complement(35313..35618)
                     /locus_tag="IXT21_000767"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ4868195.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IXT21_000768"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IXT21_000768"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ4868196.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IXT21_000769"
     CDS             complement(36724..37008)
                     /locus_tag="IXT21_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868197.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IXT21_000770"
     CDS             complement(37026..37916)
                     /locus_tag="IXT21_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ4868198.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IXT21_000771"
     CDS             complement(37936..38154)
                     /locus_tag="IXT21_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868199.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IXT21_000772"
     CDS             complement(38124..39824)
                     /locus_tag="IXT21_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ4868200.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40090..40863
                     /locus_tag="IXT21_000773"
     CDS             40090..40863
                     /locus_tag="IXT21_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868201.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="IXT21_000774"
     CDS             complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="IXT21_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ4868202.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41579..41681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41687..42658)
                     /locus_tag="IXT21_000775"
     CDS             complement(41687..42658)
                     /locus_tag="IXT21_000775"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ4868203.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="IXT21_000776"
     CDS             complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="IXT21_000776"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ4868204.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44628..45446)
                     /gene="purR"
                     /locus_tag="IXT21_000777"
     CDS             complement(44628..45446)
                     /gene="purR"
                     /locus_tag="IXT21_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ4868205.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45613..46437)
                     /locus_tag="IXT21_000778"
     CDS             complement(45613..46437)
                     /locus_tag="IXT21_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ4868206.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46410..47090)
                     /locus_tag="IXT21_000779"
     CDS             complement(46410..47090)
                     /locus_tag="IXT21_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868207.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47113..48051)
                     /locus_tag="IXT21_000780"
     CDS             complement(47113..48051)
                     /locus_tag="IXT21_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ4868208.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48296..49147)
                     /locus_tag="IXT21_000781"
     CDS             complement(48296..49147)
                     /locus_tag="IXT21_000781"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ4868209.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49301..51874)
                     /gene="mprF"
                     /locus_tag="IXT21_000782"
     CDS             complement(49301..51874)
                     /gene="mprF"
                     /locus_tag="IXT21_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ4868210.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52015..52821
                     /gene="proC"
                     /locus_tag="IXT21_000783"
     CDS             52015..52821
                     /gene="proC"
                     /locus_tag="IXT21_000783"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ4868211.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT        14649 a        11468 c         9494 g        17219 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaagct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttagtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctga ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcgcgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgt gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacatccttt aaataaaaaa tacaaaaatc acaaaaatta
    39961 attgaaaatg agagtgtttt caatgaaaac gtgattttgt aatggaattt taaaataaaa
    40021 gaaacaagct tttttaatgc aaatgttgta aaataaaaga gattaagagg aaataagagg
    40081 aggaacatga tgactgagtc agtttatgta catattgata ccacaagtaa tgctgtttta
    40141 acaaaagggc tgactgctac tgactttgca aacagtatcg tgcattttcc tcaaaacttg
    40201 ctcttgcttg atccatctgc ttctgctgga gaatatgaat cccatacagg gctgaaagta
    40261 atccgtggta cagaaaatat tcagcgtttc ttttcctctt ctcgtaacag gcgtggattc
    40321 gatgaattga agtggatcga ctttactgat ttaacgatgc tgaaagaatt gacaccttta
    40381 gaaatttcag aattattata ttttggtcat atgaaaacac atcttcattc gccgtttttt
    40441 tataaactac agaataattt tgtatacttc gatcttaatg atcagctgaa tcggatttat
    40501 tatcgctatt tagatgaatt ttataggatt tttgctagta aattaagttc gatcttgtca
    40561 gataagatca atgtaaaaaa atcttttttc aaaaaagaaa cacctgttga aaaaatcagt
    40621 gtggaattat tgaaagaaat gcgtaatttg atgcaggaag ggatcatttt ttcttttgat
    40681 caagttggtt tgatcgatgg taaatatcgt atcccaatct atgtgattga agattatatt
    40741 tggaaattaa aaggcacccg gtatcgagaa gaggaaatca tcgctacttt aatctactct
    40801 gtcaccgatc gacactggca attggtagaa gatgccgatc tttcttatct gccattgaag
    40861 tagatctatt tcttatcaaa aaataaaaag gaaacaagaa actaggacat gattctgtct
    40921 caagtttctt gcttcttttt tatttacagc tcctgtcgtt tatctggcac aaaaatcgaa
    40981 ggagtcgttt gttggatccc taataaaacg atgatcgtta caatagctgt actcaacccg
    41041 ctggcaccat tcattacgaa agaaaagagc caagaattca tcccccatag tgcatacgct
    41101 ccccagaaaa ccactcctgc aataaaatgc caaaaatatc tggaaagtgc gccaatcaat
    41161 gcaccaaaca cgatgctttt tctaatttgt ttcgtattat cgtctacata gcctttttgg
    41221 acatttgaag ccatgagccc tgctgcgcct gcaaaagtat aggcaatcgg gtactcaatc
    41281 aacacttgaa cgacacttaa ataataaact tgtcccaacg gaaaatgtag gatgccccaa
    41341 atgaaacctg ccagcatccc tgcctttgtc cctcttctta acgcaaaaag agtcaatgga
    41401 atctgcccta atgaaataga aaaactgctg ccgatctgga tcggaatcaa cgacaacacc
    41461 atcgctaacg ctgcaacaat cgttccttct acccaaactc tattttttcc cataaaaaaa
    41521 cttcctcctt tgaataaaaa agtcacaaag gaggaagcag ctttcgaaaa tagtcaaaca
    41581 gctgtttatt cacaattcct acgctcgcat aaccgataca ggtacgaagg gtttagaaat
    41641 agattcaatc tcagcccaat tggactcccc tttgtgagtt tatttattat ttattttttg
    41701 tttcaaacaa tggactaact ggtttgtttt catggatacg tttgattgca tctcccatta
    41761 gtccgccaac acttacttca tcgattttgt cgattttacg atcttctgga agatagatcg
    41821 agtctgtaac aactagacgc tcgatagctg aatcttctat tctttgtaaa gcagggccgg
    41881 ataatactgg atgtgtacat gaagcataca cactagttgc accagcttct ttcaaagcat
    41941 tggcagccaa tgtgatcgta cctgctgtat cgatcatatc atcgatcagt acacaagttt
    42001 tgcctttgac ttgtccaatg atattcatta cttctgcaac atttgctttt ggacggcgtt
    42061 tgtctatgat cgcaatcgga gatttcaaaa attccgctaa tttacgtgcc cgtgtgacac
    42121 caccatggtc tggtgaaaca acgacaacat catcgccttt gattcctttt tctaagaaat
    42181 aattggcaat aagtggcgca cccatcaaat gatcgactgg aatatcaaag aaaccttgga
    42241 tttggactgc atgaaggtcc aaagtcagca ttcttgttgc accagctttt tgaatcatgt
    42301 ttgctactaa tttcgctgtg attggttccc gtgcacgtgc tttacggtct tgacgcgcat
    42361 agccataata aggcatgact acgttgattg tttttgcact tgctcgtttc agtgcgtcaa
    42421 tcatgatcag caactccatc aagttatcat tgactgggct gctcgttgat tgaataacat
    42481 agacatgcga tccacgaata ctttcttcaa tgtttacttg aatttctcca tcactaaatt
    42541 gagtaacaga tgattttcct aactcgacgc cgactgctgc tgcaattttc tctgctaatg
    42601 ggcgattgga atttaacgca aaaattttta atcttgggtc aaagtaatgt tttgacatgg
    42661 ggacctccac tttctttttc acaactctag aattctacct aataaatagt agtcatattt
    42721 tttaaataaa tcaagtgatt ttgcaaaagt cttagtttag ataagggagt ttttttgcat
    42781 atccttcttt attgacttgt cttgctctcg caattgccag cgcatattct ggaatattgt
    42841 ctgtaattgt tgacccggca gcaatcgaac tgtttttagc tacctcgaca ggtccgatga
    42901 tattcgtgct agaaccaata aagctgtggt cgccgacagt cgtatgatgt ttgttctttc
    42961 catcatagtt gacgaagaca acaccacatc cgacattgat ttcttctccc agcgtagcat
    43021 caccaacata agtcaagtga ccgactttag ttcctttgcc gatttgagca tttttcactt
    43081 ccacaaagtt tccgatatgg acgttttcgc caatttcagc ttttggtcgt aaatgtgcat
    43141 aaggtcccac atctgcatgt ttcttgacat ggctgctttc aatgactgag ttttcaatga
    43201 cgacgtgatc ctcaatccta ctgtctacga tttttgaatg tgacccgatg acacaatcag
    43261 aaccaatgac tgtatttcct tggatttgta cacctgcttc gatcaatgtg tctggaccga
    43321 tttctacgcc tgcatcgatg tatgtcgttg ctgaatcgac aaagcttacg ccattgacca
    43381 tatgcatctg gttaattcgc tttctcatga tctcatttgc tttagctaac gcgatccgat
    43441 cattgactcc cattgattct tcaaaatcat ccgtttgata agcagcaact gtatggcctt
    43501 cttcttttaa gatttcgatg atatctgtta gatagtattc accttgagca ttgtttgttc
    43561 caactttgct taacgcatcg aacaaagctt cgttgtcaaa acagtaagtt cctgtattga
    43621 tttcttgtac aagagcttct tctggtgttg catctttttg ttccacgatt ttttctacga
    43681 tcccaatatg gtcacggatg attcgtccat atcctgttgg gttttctgct tgtgccgtca
    43741 agatcgttgc acttgcattt tttccttggt gatattcaaa aagattgttc agcgtttcag
    43801 tagtcaataa tggtgtatcg ccactgataa ctaacgttgt tccttttttt ccttccaaga
    43861 aagaagcagc ttgcaaaaca gcatgtcctg ttcccaattg ttctgcttgt aacgcatatt
    43921 tactgcgttc tcctagttgt gctttaactt gttctgctcc gtgtccaacg atcgtaatca
    43981 cttcatctgg tttcgtttca ctgacccgat taatgatatg ttctaccata ggtttaccag
    44041 aaacgggatg taaaacttta tataattttg acttcatgcg ggtacctttc cctgccgcca
    44101 aaataattgc ataacgtgcg tccaactgcg tcactcctaa atatagtttg ctgatttcag
    44161 tttatcccag tatacccatt ttttcaacaa aacaagtgat tttcttttat cccagcgttt
    44221 tctggataaa aaaatagata gaccataacg ggcctatcta tttggtaacc ggtgctctcc
    44281 tgccaactcc tcggtcacaa gtcacactac tcataaaaca tgaaaagctg ttttttgaaa
    44341 tcgtttcttg tgacttagag ttaaacggta ggcttacaac ctcttcatta aacgtttttc
    44401 aatcagctga ccttcggtat tagctcaata aacggcaaaa tatgaggagc aatttttccg
    44461 tttattgagc taatactcag gacaggacgc tgatttcaca acctctagat aaacggtgtt
    44521 caaaagccag tttctcggta ttaagccaaa tttatgaaaa aatgtggaaa gccattttca
    44581 caaaattttt cttactactc gaaactaagc ggctttttca caacctctta ttctgaaaaa
    44641 taatttcctg gcacaaccgt aatatttttc gtttgagtat ctacatcttt gacataaagc
    44701 agtgacgtat aatcatcgat ggcgcgatga ccgttgaatt ttgattcggc aaatacagtg
    44761 atgccaacaa gttctgattc gaattcttca atcaagctct tcattccgtt gactgttccg
    44821 ccgcctttca tgaaatcatc cactacaagg acacgtgaac cacgtttcaa acttctctta
    44881 gacagttcca tcttttcaat tctttcagaa gatccagaca catagttgac acttactgtt
    44941 gatccttctg ttatttttga gtcccggcgt acaataacga atggcgcatt cagataatag
    45001 gacacagctt gggcaatagg tacgcctttt gtggctacag tcattacagc atcgattttt
    45061 tcaccaaggt atttagaagc aatgattctt ccaacttgac gcagcagttc aggttctcct
    45121 aacaaatcag agagataaac atatcctcct ggaagcagac ggtcttgttc tgatagacgt
    45181 tctgctagag attcaatata ttttttcgcc tcttcatatg aaatttcagg gatgaacaat
    45241 acaccacctg cagcacccgg gattgtttcc aaggttccat accctcgttc tttaaatgtt
    45301 ttcttgatga ttcccaaatc ttcactgata gaagatttag cagattcata acgttcggca
    45361 aagcttgtta aggaaatcaa ttcatgcggg tgattcaata aatactgtgt catgtcaact
    45421 agacgttcac tgcgacgtac tttcacctat tccaactcct tttaagaaat tttatcgttt
    45481 tacattatat aagtttttct tcaaaaattc gagcatttta tgaaaaaata agaaaatagt
    45541 tcggtattta ttaaaataaa aagaagccta taaaaaattt ttcgttttta taaggcttct
    45601 tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt
    45661 tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag
    45721 ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc
    45781 aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat
    45841 tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat
    45901 cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc
    45961 atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac
    46021 gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag
    46081 ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag
    46141 cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt
    46201 tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg
    46261 attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc
    46321 agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc
    46381 aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga
    46441 gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga
    46501 tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga
    46561 taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag
    46621 tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat
    46681 tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca
    46741 tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa
    46801 cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga
    46861 atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct
    46921 tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt
    46981 tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca
    47041 ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct
    47101 ccattctaaa acttaattga tactttcttt taaagctttc agattttttt ccatgacaga
    47161 aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac
    47221 ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc
    47281 ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg
    47341 acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta
    47401 ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc
    47461 ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc
    47521 attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt
    47581 tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga
    47641 atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc
    47701 gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga
    47761 atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc
    47821 tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag
    47881 tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt
    47941 cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc
    48001 aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct
    48061 cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg tatcaaaata
    48121 aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta
    48181 aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat aaaaaactga
    48241 agaataccat tttaggattc ctcagttttt catcacctat tttacttctt atattttatt
    48301 gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta tatactctct
    48361 gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca ctcatcaatg
    48421 cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact ggatgccgct
    48481 ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga taatcttgct
    48541 ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc gtatctactt
    48601 ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca acccagcact
    48661 gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat gccgtacttc
    48721 cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc agttcttcta
    48781 acgaacagcc cagattccat aattggttga tccctcttag agcagctgca cagtctgtac
    48841 ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc ttgacaccgg
    48901 ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg ttccgttcat
    48961 ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt atttcttcaa
    49021 aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca tgatacccat
    49081 cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct ttttcaatca
    49141 attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca tagggaaaaa
    49201 taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa cagctttcac
    49261 agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct gtgagcactt
    49321 cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt agctgcatgg
    49381 tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc caatgatcga
    49441 catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta ccataattat
    49501 atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca acattagcta
    49561 atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca ataaacagat
    49621 tggctaacag atagtcagct aattcctcag attctttagg taagacccgc aacaaatcat
    49681 atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact ttttgatctt
    49741 tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt cccacagaga
    49801 agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca gcttgatgta
    49861 attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc aaacgttgat
    49921 attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca atccctgctt
    49981 cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag ctttctgata
    50041 tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac tgattcgttg
    50101 ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat ttgtttcctt
    50161 tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa aaataactgt
    50221 aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc aataaacggt
    50281 caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct aaatattgat
    50341 aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag aaccacacat
    50401 catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa aagcgtccgc
    50461 tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta gcatcaaaga
    50521 ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg gataaccaca
    50581 cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta cgtgtgattg
    50641 tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt ttgactttta
    50701 ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc actgtctgat
    50761 ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt gcagacagat
    50821 tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca cttacgagca
    50881 cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat cgattgattt
    50941 tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga acataataaa
    51001 acaaccgata atataatagc caaactactg ctacatcttg ccgaacagac aattgactca
    51061 ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc gtcagcattc
    51121 ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata tctattctca
    51181 tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca aaggatgcca
    51241 gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt ctctttagat
    51301 aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag atccaatagc
    51361 ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga aacgaccata
    51421 cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa accatttttc
    51481 tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca ccaccaaaac
    51541 cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct tttctttcca
    51601 tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac aacaacatcg
    51661 gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg ctttgttgtc
    51721 ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg acttgatttg
    51781 ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta tgttccttta
    51841 tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa cttgtatact
    51901 tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta gacctgttct
    51961 ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga taacatgaaa
    52021 gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt attaaaaaaa
    52081 ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa tgcacaaaaa
    52141 tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc atcacaatca
    52201 gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga agaactaaga
    52261 gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt aacagattta
    52321 gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa tgtcaatgtc
    52381 agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga ggaacagctt
    52441 cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc agaatcagat
    52501 ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat gctgatcgat
    52561 gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac tgagattgct
    52621 gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa tccatgggaa
    52681 cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt tgccttagaa
    52741 gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga gaaagatctt
    52801 gagatgttga ataaaaaata atgatagcaa
//
LOCUS       DACGLH010000012        51933 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.013, whole genome shotgun sequence.
ACCESSION   DACGLH010000012 DACGLH010000000
VERSION     DACGLH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.013"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            564..1253
                     /locus_tag="IXT21_000784"
     CDS             564..1253
                     /locus_tag="IXT21_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4868212.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1240..2448
                     /locus_tag="IXT21_000785"
     CDS             1240..2448
                     /locus_tag="IXT21_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4868213.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2742..4013
                     /gene="serS"
                     /locus_tag="IXT21_000786"
     CDS             2742..4013
                     /gene="serS"
                     /locus_tag="IXT21_000786"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ4868214.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4562..6046)
                     /gene="guaB"
                     /locus_tag="IXT21_000787"
     CDS             complement(4562..6046)
                     /gene="guaB"
                     /locus_tag="IXT21_000787"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ4868215.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6551..7246)
                     /locus_tag="IXT21_000788"
     CDS             complement(6551..7246)
                     /locus_tag="IXT21_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ4868216.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7336..8436)
                     /gene="ychF"
                     /locus_tag="IXT21_000789"
     CDS             complement(7336..8436)
                     /gene="ychF"
                     /locus_tag="IXT21_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ4868217.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8445..8636)
                     /locus_tag="IXT21_000790"
     CDS             complement(8445..8636)
                     /locus_tag="IXT21_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ4868218.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8657..9547)
                     /locus_tag="IXT21_000791"
     CDS             complement(8657..9547)
                     /locus_tag="IXT21_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ4868219.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9534..10301)
                     /locus_tag="IXT21_000792"
     CDS             complement(9534..10301)
                     /locus_tag="IXT21_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4868220.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10313..11026)
                     /gene="rsmG"
                     /locus_tag="IXT21_000793"
     CDS             complement(10313..11026)
                     /gene="rsmG"
                     /locus_tag="IXT21_000793"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ4868221.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11258..13159)
                     /gene="mnmG"
                     /locus_tag="IXT21_000794"
     CDS             complement(11258..13159)
                     /gene="mnmG"
                     /locus_tag="IXT21_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ4868222.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13176..14573)
                     /gene="mnmE"
                     /locus_tag="IXT21_000795"
     CDS             complement(13176..14573)
                     /gene="mnmE"
                     /locus_tag="IXT21_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ4868223.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14917..15255
                     /locus_tag="IXT21_000796"
     CDS             14917..15255
                     /locus_tag="IXT21_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868224.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15423..16181)
                     /locus_tag="IXT21_000797"
     CDS             complement(15423..16181)
                     /locus_tag="IXT21_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ4868225.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16199..17017)
                     /locus_tag="IXT21_000798"
     CDS             complement(16199..17017)
                     /locus_tag="IXT21_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ4868226.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17014..17370)
                     /gene="rnpA"
                     /locus_tag="IXT21_000799"
     CDS             complement(17014..17370)
                     /gene="rnpA"
                     /locus_tag="IXT21_000799"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ4868227.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17488..17622)
                     /gene="rpmH"
                     /locus_tag="IXT21_000800"
     CDS             complement(17488..17622)
                     /gene="rpmH"
                     /locus_tag="IXT21_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ4868228.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18145..19479
                     /gene="dnaA"
                     /locus_tag="IXT21_000801"
     CDS             18145..19479
                     /gene="dnaA"
                     /locus_tag="IXT21_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ4868229.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19677..20807
                     /gene="dnaN"
                     /locus_tag="IXT21_000802"
     CDS             19677..20807
                     /gene="dnaN"
                     /locus_tag="IXT21_000802"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ4868230.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21036..21278
                     /gene="yaaA"
                     /locus_tag="IXT21_000803"
     CDS             21036..21278
                     /gene="yaaA"
                     /locus_tag="IXT21_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ4868231.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21265..22389
                     /gene="recF"
                     /locus_tag="IXT21_000804"
     CDS             21265..22389
                     /gene="recF"
                     /locus_tag="IXT21_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ4868232.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22386..24332
                     /gene="gyrB"
                     /locus_tag="IXT21_000805"
     CDS             22386..24332
                     /gene="gyrB"
                     /locus_tag="IXT21_000805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ4868233.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24352..26823
                     /gene="gyrA"
                     /locus_tag="IXT21_000806"
     CDS             24352..26823
                     /gene="gyrA"
                     /locus_tag="IXT21_000806"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ4868234.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27059..27358
                     /locus_tag="IXT21_000807"
     CDS             27059..27358
                     /locus_tag="IXT21_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ4868235.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27408..27920
                     /gene="ssb"
                     /locus_tag="IXT21_000808"
     CDS             27408..27920
                     /gene="ssb"
                     /locus_tag="IXT21_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ4868236.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27946..28182
                     /gene="rpsR"
                     /locus_tag="IXT21_000809"
     CDS             27946..28182
                     /gene="rpsR"
                     /locus_tag="IXT21_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ4868237.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28325..30298
                     /locus_tag="IXT21_000810"
     CDS             28325..30298
                     /locus_tag="IXT21_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ4868238.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30304..30756
                     /gene="rplI"
                     /locus_tag="IXT21_000811"
     CDS             30304..30756
                     /gene="rplI"
                     /locus_tag="IXT21_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ4868239.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31030..32397
                     /gene="dnaB"
                     /locus_tag="IXT21_000812"
     CDS             31030..32397
                     /gene="dnaB"
                     /locus_tag="IXT21_000812"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ4868240.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33177..34469
                     /locus_tag="IXT21_000813"
     CDS             33177..34469
                     /locus_tag="IXT21_000813"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ4868241.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            complement(34748..35458)
                     /locus_tag="IXT21_000814"
     CDS             complement(34748..35458)
                     /locus_tag="IXT21_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ4868242.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(35455..36927)
                     /locus_tag="IXT21_000815"
     CDS             complement(35455..36927)
                     /locus_tag="IXT21_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4868243.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            37065..37538
                     /locus_tag="IXT21_000816"
     CDS             37065..37538
                     /locus_tag="IXT21_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4868244.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(37691..38242)
                     /locus_tag="IXT21_000817"
     CDS             complement(37691..38242)
                     /locus_tag="IXT21_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4868245.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            38347..38877
                     /locus_tag="IXT21_000818"
     CDS             38347..38877
                     /locus_tag="IXT21_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ4868246.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(39360..39803)
                     /locus_tag="IXT21_000819"
     CDS             complement(39360..39803)
                     /locus_tag="IXT21_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4868247.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(39819..40979)
                     /locus_tag="IXT21_000820"
     CDS             complement(39819..40979)
                     /locus_tag="IXT21_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4868248.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            41486..43186
                     /locus_tag="IXT21_000821"
     CDS             41486..43186
                     /locus_tag="IXT21_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868249.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(43288..44121)
                     /locus_tag="IXT21_000822"
     CDS             complement(43288..44121)
                     /locus_tag="IXT21_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ4868250.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(44425..46590)
                     /locus_tag="IXT21_000823"
     CDS             complement(44425..46590)
                     /locus_tag="IXT21_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4868251.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            46850..49144
                     /locus_tag="IXT21_000824"
     CDS             46850..49144
                     /locus_tag="IXT21_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ4868252.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            49137..49802
                     /gene="pgmB"
                     /locus_tag="IXT21_000825"
     CDS             49137..49802
                     /gene="pgmB"
                     /locus_tag="IXT21_000825"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ4868253.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            49795..50802
                     /locus_tag="IXT21_000826"
     CDS             49795..50802
                     /locus_tag="IXT21_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ4868254.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            50824..51843
                     /locus_tag="IXT21_000827"
     CDS             50824..51843
                     /locus_tag="IXT21_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4868255.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT        15813 a        10288 c         9951 g        15881 t
ORIGIN      
        1 gccgtagcaa cttttatatc atatcagaga gacaagtaat tgtcaactct tttttaaaag
       61 ttttttcgat ttcgtttttc gaaatcttcc agcaactcat ttatcataac ttatcgtttg
      121 ttatttgtca agaacttttt tgaagttttt ttcgatagaa tcaagcttcg caagtggttc
      181 ttttttaacg actcagttat cttatcatgt catttccaac atgtcaacaa cttatttttt
      241 attatttaat aagttattgg tgttcttttg aacaaataat aatatatcaa catcacatgt
      301 gaaagtcaat aagaaaatgc ttatttatca acatttctag cttacatgat cattggttag
      361 ttctggtcct ttttcgttcg tctttataac tccgagagac gtaaaatcct tttgttttca
      421 gtttaatgtt catgttcttg tcttattcac accaactaga tgtctcacct tctaaaattt
      481 tccttaacaa agtgctgctt ttatctattt ctgctttttt ttgctagaat aatagtaaca
      541 atatgactct ggaggattta tttatgaaaa ttttgattgc tgacgatgat aaagaaatcg
      601 ttgaattatt aagtatctat gttcacaatg aggggtatga agcagtaaag gcttatgatg
      661 ggaaagaagc attatctaaa atcagaacaa tccctgatat tgaattactt attttagata
      721 tcatgatgcc tgagatggat gggatgcaag tagtgaaaga gctacgaaaa gaatcccaaa
      781 taccaatcat tatgctgaca gcaaaaacta ctgatatgga taagatcaaa ggactggtcg
      841 ctggtgcaga tgattatgtg accaagccat ttaatccgtt agaagtaatg gctagagtaa
      901 agtctttatt acggcgtaca aaaatgcaga ttgccccaga tcagcctgat gtattagaag
      961 taaatgcatt aatcatcaat aaagactctc atgaagttaa aacaatcgat gggaaagaaa
     1021 tccagttaac agctttggaa ttcggtatcc tctacttgct tgctagtcat cctaatcgag
     1081 tatttagcgc ggatgaaatt tttgagcgtg tttggaaaca agaaagtatt gtctctgcta
     1141 aaactgtcat ggttcacgtt agtcatttaa gagataagat tgaagaagca actggtggtg
     1201 aaaaagtcat tcaaaccgtt tggggagtgg gctataaaat tgacgcaaga tagtaaacga
     1261 gcaactgaaa aacgccgtcg gatcaatttg acttcaaaag aaatcagcga attgttagcg
     1321 gaaggaatca tcacgattat ccttttgctt ctattaaatg tttctattct ggttgttatc
     1381 agttcggtga tcaacagttc accttctttg acgaatgcga tttgggattc aaagaatatt
     1441 tttgcagaac gattgaacac tgatttgttc tggaacggcc ggaattttat tattcccttt
     1501 ttctttctat tagatattgg tgttttgtat tggcgcttga ttcgaagata tcgccaaatg
     1561 caattgcggc atatcatcag cgaacttcac tacatcgcaa acggaaatta tgatcatcgt
     1621 atcccttttg aactgagtgg tgacttaagc cgagtggtaa caagtatcaa tggattggtg
     1681 gatagtaccg tagcagcgat tgaagacgaa cgtaaaatcg aaaaatcaaa agatgaattg
     1741 atcacgaatg tcagtcatga tatccgtacc ccactcacgt ccatcattgg atacctagga
     1801 ctgatagaag atggccagta tcactcagaa gaagatcttt taaagtatac gcatactgct
     1861 tatatcaaag caaagcagat gaaatcttta gtggacgact tatttgaata cactaaggta
     1921 cgccagcctg ctgtcccggt gaatttttct gcctttgaca tgatccagtt gatcgaacag
     1981 ttagcagctg attttgagtt ggaagcttct aaaaaaaata tccaaatact tgttcaatca
     2041 aaagtagact cccttatcat ggatggcgat accgaaaaac ttgtacgtgt cttcaataat
     2101 cttttgacga atgcgttgaa atacggaaaa ggagcaacga agatcgtaat cgaagtagaa
     2161 cgtatcggtt cagaagttgt tgcaacagtc aaaaataacg gagcaatgat cccacaacag
     2221 gcaatcgata atctattcga ccgtttttat cgtgtagaag aatctcgttc acaagcaact
     2281 ggaggaactg gacttggttt agcaatcgct caaagcattg tggctttgca cggcggatac
     2341 atttatgcaa aatcagataa gcaatggact tctttcatca tgcaccttcc aatcaagaaa
     2401 aatgaaaaat tacctattga tacacaggaa gttattgacg aaagctgaaa tattcgctat
     2461 tcttattaca attattagaa taaatgaaaa acacagaacc cagtacactt tattcttttt
     2521 cagagagctg atggcaggtg taaatcagca aaggataatt tggaagttcc agttcgttta
     2581 cgtgcccttt cgaaataaga aaggtcggtt caacagccgt tatgtgttca acaagtgcag
     2641 ctgacgctga acttgggtgg taccgcgaat ttcagactga ttcgtcccaa gaataatggg
     2701 atggatcagt ctttttttat tttcaaaaag gaggaaatca tatgttagat gtaaaaatga
     2761 tgcgacaaaa tattgaagaa gtacaagaaa aattgatgac tcggggcgta aaaaaagaaa
     2821 cattagctga attcatggaa ctagatgaaa atcgtcgtaa gctactagtc aaaagtgaag
     2881 aattaaaaaa atatcgaaat gatgtttctg ctgagatcgc ccaattgaag cgtgcaaaag
     2941 aagatgcttc tgctaaaatc gctgaaatga aagaagtcgg tggcaacatc aaagcattgg
     3001 atactgaaat tgctgaagtg gatgaaaaat tgaaacaaat tgcaactact ttgccaaacc
     3061 ttccccatga ttctgtgcca gttggaacag atgaagagga caatgtagaa atccgtcgtt
     3121 ggagcgaacc acgtacattt gcttttgaac caaaaccaca ctgggaaatt gcagaaaatc
     3181 ttgatatttt agattttgaa cgtggcgcaa aagtagccgg tagccgtttt gtctattaca
     3241 aaggattagg tgctcgatta gaacgtgctg tctacaactt catgcttgat cagcatattt
     3301 acgaacacgg ctatactgaa atgatcactc cttatatcgt caacagtaaa gcgatgtttg
     3361 gtacgggaca atttccaaaa ttcaaagaag atgttttcca attggagaat actgatctaa
     3421 cattaatccc aacagctgaa gtaccattaa ctaactatta taatgatgaa attttagatg
     3481 gtgcagattt acctatttat ttcactgcac taagccctgc ttttcgttct gaagccggaa
     3541 gtgctggtcg ggatacacga ggactgattc gtcttcacca attcaataaa gtagaaatgg
     3601 tcaaattttc ggatgtagag cattcttacg aagaattgga aaaaatgaca gctgatgcag
     3661 aagatatctt gaaaaaattg catttgcctt atcgtgtgat ggccttatca actggagaca
     3721 tgggcttttc tgcagctaaa acttatgact tagaagtatg gatacctgca caagatactt
     3781 atcgtgaaat cagttcttgt tctaactgtg aagatttcca agcacgccgc gcaatgatcc
     3841 gttatcgcga taatgatgga aaaatccact atactcatac tctgaatggt tcaggacttg
     3901 cagttggccg tacagtagct gccattttgg aaaactacca aaatgaagat ggttctgtca
     3961 cagttccaga agcgctcgtt ccttatatgg ggaatctgaa agttattaaa taaagaggct
     4021 gtgaaaaagc cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     4081 ttttgcataa attggactta ataccgagaa actggctttt gaacaccatt tatcgagagg
     4141 ttgtgaaatc agcgaattaa cctgagtatt agagaaaaaa atggaaaaat agtccctcat
     4201 attttaccgt ttgttgaact aataccgagg gtcagctgat tgaacaccgt ttagtggaga
     4261 ggtcgtaagc ctacccccca gctcctagtt acaaggacaa attcaaaaaa cagctcttca
     4321 tattttatga atgttgtact tgtgattcgg aggtggcaag ggaacactgt ttattcaaaa
     4381 agaggctggg acagaagcgt ttaactccaa gaaataagaa gaaattcacg aaaattgctt
     4441 ttcaaatttt tgtgaatttc ttcttatttc cgaaggagtt gcttctactt ccgccgttta
     4501 tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct ctttaatgtt
     4561 tttaagattc aactgaatag tttggtgctt cttttgtgat ttgtacatca tgagggtgtg
     4621 attcttttaa tccactgccg ctcatttgga taaattgcgc ttcatcacgt aattgctgta
     4681 gattggctgc cccaacataa cccattcctg aacgcaaacc gccgatcatt tggaaaataa
     4741 tatctgcaac acttccttta taagcaaccc gaccttcgat tccttctggt actaatttat
     4801 ttgcttcatt gacactacct tggaagtaac ggtcttttga acctttttcc atcgcgccta
     4861 atgagcccat acctcggtat gttttgaatc gacgtccttg gaagatttcg aattcacctg
     4921 gtgattcatc tgtccctgcc aacatacttc ctagcatcac tgcatgtccg ccggcagcta
     4981 atgctttgac gatgtcacca gaatatttga tccctccgtc tgcaataatc gctttgccat
     5041 attgacgagc aactgatgct gcatcataaa tagctgtgag ctgaggtaca cctacaccgg
     5101 ctaccacacg tgtcgtacaa atcgatcctg gtccgatacc aacttttacg acatctacac
     5161 ctgcatcata taaagctttt gtcgcttcag ccgtcgcaac gtttcctgca atcaatgttg
     5221 catctgcaaa agttgaacgg atttcttgaa tttttcggat cacacctgca ctatgtccat
     5281 gagctgtatc gatgatgatt gcatctgccc ctgcttctaa taaagcattc gcacgctcaa
     5341 atgtatcgct tgttacacca acggctgctg ctacaagcaa tcgaccatgt tcatcttttg
     5401 ctgcatttgg aaattcgatc actttctcga tgtctttgat cgtgatcaat ccgcttaaac
     5461 gaccttcgtt atctacgatt ggtaattttt cgattttatg tttttgcaaa atcttttctg
     5521 catcttttag agaggtacct actggggcag tcactaagtt gtcttttgtc atcacatcag
     5581 caatcggcat gtgataatct gttacaaaac gcatgtcgcg gtttgtaatt atccctacca
     5641 attttctgtt ttcaagtgtt tcaacgattg gcacaccact gatacgatat ttgctcatca
     5701 attcttctgc gtctgctaca agatgctgtg gcgttaagaa gaacggatca atgattacgc
     5761 cactttcaga acgtttgact tttcttactt catccgcttg ctggctgatc gtcatgtttt
     5821 tatggatcac acctagtccg ccttgtcttg ccatagcaat ggccatcttg ctgtcggtaa
     5881 cagtatccat acttgcactg atgattggaa tattcaactt aatattttta gctaattgta
     5941 cgctcatatc cacttcgttt ggtaacacgt gactttctgc tggtatcaat aagacatcat
     6001 caaaagtaaa tccttttttc gtgaactttg tttcccagtt ggacattttt agtaccactc
     6061 ctctaattta gtttatctat taaaataatt gattttttca tttaagtcaa tgatctactt
     6121 ggaatcaagt tagctttctc atagaatacc aatataaagc gtttacatct gtgtatatta
     6181 aaagagattg taaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
     6241 ttctcatgtt tttgaattgg ttgggtttat taccgcagat gcaaactttt gaacaccgtt
     6301 tagtggagag gctgtgaaaa agctgcttag tttcgagtag taagaaaaat tttgtgaaaa
     6361 tggctttcca catttttgca taaattggac ttaataccga gaaactggct tttgaacacc
     6421 gtttatcgta aaaagggact gtgacaagac ttttgtcaca gtcccttact attatttaac
     6481 tatggataaa caaaatgagg ttctttacta cccctagata tctagcaaaa tagctgatct
     6541 ttttgctagt ttatcttctt gggacggata aacttccaat aatcccgtat tttttcttca
     6601 agtagtaacg gattgctaaa gcagttgccc cgataatgat aactactact ggatctaata
     6661 ctggattgat aactggtggc aatagtgttg tcgcagaaaa cagtacgacc caaagaagtg
     6721 tcgttccacc taggataagc aatgtcttac ccattcctgg tttcttgctt cgatctgctc
     6781 cgggatattc atattgatag atgtatttgt acatcatata aaagacccag ccaccgattg
     6841 cagaacctag aacgagtgta gaaaagccat aagctggcgt tgttccacgt gaaaacaatc
     6901 cggtaatcgc aatcatgata cttaatagac caagcaacag caacgtatta tctaaccaca
     6961 tcaataaagg ggtggatgaa ttttttactt ctgctggctt attgatgatt gcttccgttc
     7021 tttcagagac tgtcccaaac aattgacggg ctgttcttcc gcctttttgt tctgcaacta
     7081 aagtaggcag aatgtcatga agggctttta ctttttcttc ttcagacaaa ttcgctgctt
     7141 ctaaagattt tttcaaatca aaaatgtact gttgatttct tttggttaat tttgtttcta
     7201 aactattgtt ttcttttacg attgctcgta attgttctgg ttccattatc taatagtcct
     7261 ccttagccgt tccttttctc aacggtcgtc acaaaagaca ttctaccata ttctacagct
     7321 agtttgatag cccttttaca cattaaagcg gaataacatt acgtcgccgt cttgtacgac
     7381 ataatctttt ccttctaatc gaacacgtcc agcttctttt gctgcatgca tgtttccgta
     7441 atgatccaag tcttcaaaag agacagtttc tgcacggata aatccgcgtt cgaaatctga
     7501 atggatgatt cctgctgctt gtggtgcttt gattcctttt ttgaatgtcc aagcacgtac
     7561 ttcttgttcc ccagcagtaa agtaagtagc taagcctaac aaatcataag cagcacggat
     7621 caactgatcc aaacctgatt cttcgattcc taaagcttct aaaaattccg ctttgtcttc
     7681 ttcatctaat tctgcgattt cttcttctgc acgagcactg accacgatca cttctgcatt
     7741 ttcttctgca gcaaagttgc gcacttcttg tacataatga ttcgcgtctg catctgctac
     7801 gtcatcttct gagacattgg ctacataaag aattggtttt gtcgtcaata aaaatagtga
     7861 tttaacgatt ttttgttctt cttcagtaaa ttcgatcgtt cgggcagatt ttccttcttc
     7921 cagtacaggt ttgattttat ccagtacagc tagttctgcc actgcatctt tatctttggt
     7981 tttagcaact tttgctacac gagtatagcg tttgttgata gattctaagt ctgctaacac
     8041 tagttccaag ttgatcgtat caatatcttc tagtggatca acacgtcctt ctacgtgagt
     8101 aatattatca tcatcaaaac agcgaacaac gtgacaaata gcgtctactt ggcggatatg
     8161 gcttaagaat tgatttccca aaccttctcc tttacttgcg ccttttacga ttccagcaat
     8221 atctgtaaat tcaaaagtcg tcggaacagt ttttttcgga tgtacaagtt ctgttaagcg
     8281 ttgcaaacgc cagtcaggta cttctaccat ccctacgttg gggtcgatcg tcgcaaaggg
     8341 atagtttgct gcttctgctc ctgcttttgt gatcgcatta aacaaagttg attttcctac
     8401 gttgggcaac ccaacaattc cagcagtcaa tgccataaat ttattcactc tttctctgca
     8461 tttttttcaa ttactttttt taatttcttt tcaaaatctc gacgggtcat catgacgata
     8521 tgtccgcaat tcatacatct gattttgata tctgcgccca ttcgaatgac ttcccagcga
     8581 ttcgtctggc aagcgtgagg ttttttcatt tcaacgatat cgtgcaagtc gtacataaaa
     8641 atagcctcct ttttgtttat tcttcatcaa attgaatatt caagatatca agaattcgag
     8701 tcaaatcttt ttgagaaaga tattcaatct cgatcttacc tttcccattt ttttcttgga
     8761 tagctacact tgtaccaaat ttatccatca aacgatcttc gctttctcgg atataatatg
     8821 gtttttcctt tgctagcctt gggatttttt tcttttcttt tccatcattt tgatttaact
     8881 cagaaacaag ctgttccagt tggcggacag ttaaattttc agttactgca cgattggcta
     8941 atttcaagat caatgtttta ttttttaaac ctaacagcgt acgggcttgt cccatagata
     9001 aacgctgatc ttgtaccatt tccttgacaa gatccggtaa agaaagcaag cgcaaataat
     9061 tggcgatata aggtctgctc tttcctaaac gactagctac ttctgcttgt gttagtttca
     9121 ggtttttcat cagcatctca tatgcttctg cttcttctaa cggattcaga tcttctcgct
     9181 gcaagttttc cagcactgct acttgcatca tcgcttcttc gtcaaagtct ctaacgatag
     9241 cagggatcgt ttctttttca gctagtttgg acgctcgaaa acgtcgctcc cctgcaataa
     9301 tctcatatcc ttttactgcg gaacggcgta cgatgatggg ttgaaagaca cctgattgtt
     9361 caatagaatt agctagttcc tgcaaagata cttcttcaaa tgttttacgg ggctgatacg
     9421 gatttggtcg cagctcattc aatggtagct ggacaacggt atcatctttt acatccaccg
     9481 tctctaagtt ttctaaatct tgaaacagtg catcgattcc tcggcctaat cctttacttt
     9541 tcttcacgac ttaccacttc ctttgccaat gcttggtaga cttcagctcc tcgagaacgt
     9601 ggatcgtagt cgatgatcgg ttttccatga ctaggtgctt ctgataaacg aatatttctt
     9661 ggaatgatcg tttcgtaaac tttttcgcgg aagtatttac gtacttcttc tacgacttca
     9721 tttcccagat ttgttcgtgc atcatacatt gtcagtaaga ctccctcgat ctctaattct
     9781 ggattaaagt gtttttggac taaacgaacc gtattcagta attggcttaa tccttccaat
     9841 gcgtaatact cacactgaac aggtatcaaa atcgaatcac ttgctgtaaa tgaattgatt
     9901 gtcagatggc caagagaagg cgggcaatcg atcaaaatat aatcatactg actgcttact
     9961 tctgctaaag atccttttaa tcttgattct cttgccatca ttgacgtcaa ttcgatctct
    10021 gctcctgcta attggagagt cgcaggcaca atactcaaat tttcgtgttc agtgatcaaa
    10081 gttgcctcat caattggcaa ttcattgact agaacatcat agatgtctct tgtcacatca
    10141 ggttttctga tcccgacacc actggtagca tttccttgtg catccatatc cactagcaat
    10201 acttttttgc caaggctagc aagacaagca cctagattca ccgtcgtcgt tgttttgccg
    10261 acgccacctt tttggttcgc aacagatatt atttgtgcca tctcttctcc tcctatttga
    10321 ttggctgctt attaggcagt ccgggctttc ttgggtattt ctttggggtt ggtttctttt
    10381 tttgaataac gatgatatgc cgttgatcag agacattcgg caaggtaacc gcctcttctt
    10441 tgaggaattt accacctaat aaagcaatcg cttgttttgc ttctgtcaat tcctcttcac
    10501 tttttgctgc tttcaatgca taaaaatagc cttcttgttt agccaagggc aaacacaatt
    10561 ccgctaacac attcaaacga gcaaccgcac gagcagtgac aaggtcaaat gatgcacgaa
    10621 agtgaggatt ttgcccaaat gtttctgccc ggtcatgata taaggaagca tcgattccta
    10681 ataatcgggt taactcagat aagaaggtta ttcgtttatt cagagaatca acgatcgtta
    10741 tctgcagttc tggaaaggca atttttaaag gtatgctagg aaaccctgcc cccgaaccaa
    10801 catcgcataa agataaaggt tgcgtaaagt ctacttcaaa tgctaatgta agtgaatcgt
    10861 aaaaatgctt cagatatacg tctttttcat ccgtaatagc tgtaaggttc atcttttcgt
    10921 tccattcgac taatgtccga taataaatag cgaattgatt catttgatga tcggtaagct
    10981 ctatcccttt ttctgccaag gattggcgaa attcttctgg tatcataaat attcctttca
    11041 ttgtgaaaca gcaattgttt cacagattct ctccctactt taacgcaaaa tctctagaca
    11101 tgcaatcctt ccatatggat tcttaaaaat ttgaaaagta agaaaagccg atagactttt
    11161 tctttctagt atgactcata aaatatacac agaagagaga actagctttg agcaatttct
    11221 gcaaaaacta gttctctctt ctatttatta ttacgtatta cgctggttca ctgactttcg
    11281 caatcttccc ttgttcgata tagaccatta ggatacttag gtctgcaggg tttacgccac
    11341 tgattcggct agcttgggca attgtttctg gttggatttt ctgcaatttt tgttttgctt
    11401 ctgttgctaa tccgttgatt gctgcgtagt cgatgttttc tggaatcttt ttcgcttcca
    11461 tgcgttttaa tttttctact ttttccatgg cttttttgat gtagccttca tatttgattt
    11521 ggatttctac ttgttcgatt tcttttcgtg tcaattcttc attttctgga ataaaccgta
    11581 gaagttctgc ataagtaatc tcaggacgtc ttaaaaagtc gcttgctagt acaccatctt
    11641 ttaatggtgc cgcattaatc gttgctaaaa agtcttggac ttcttctgtt ggtttgattc
    11701 gtacattcat caaacgtttg atttcttgtt caactgcttc ttttttcttc aaataattct
    11761 tgtattgatc ttcttttact aagcctacac ggtgacccat ttctgttaat cgcaaatctg
    11821 cattatcatg acgtaaaagc agacggtatt cagcacgtga agtcagcaaa cgataaggtt
    11881 cgttcgttcc ttttgttacc aaatcatcga tcatgacacc gatgtaaccg tcacttcgtt
    11941 tgatcactaa aggttctttt ccctgaactt tcaaagctgc attgataccg gcaatcagcc
    12001 cttgtccagc cgcttcttca taaccgcttg taccatttgt ttgtccagct gtataaagat
    12061 tttcaacgac tttagtttct aatgtcggac ggagctggtg tggctctact acgtcatatt
    12121 cgatagcata tcctgtacgc atcatttcta ctttttctaa tccagcaata gaatgaagca
    12181 tgttgacttg aacatcctct ggtaaagaag tagataaacc ttgaacatag acttcttccg
    12241 tatttaagcc ttctggttcc aaaaataatt gatgacgtgg tttgtctgca aaacgcacga
    12301 tcttatcttc gatggatgga caatagcgag cgcctacccc ttcaacgatc cctgtaaaca
    12361 ttggggcacg atgtaagttg tctcgaataa tttggtgagt cgtctcacct gtataggtca
    12421 gccagcaaga ttcctgatct agtttatagg cactatctgg tgtgctgaag ctaaaatgat
    12481 tcggctcttt gtcaccaggc tgctcttctg taacagaata gtcgatcgta cttgatttta
    12541 ctcgaggcgg tgttcctgtt ttgaaccgtt cgatagcaaa gccaagttct tttagatgat
    12601 tcgctagtcc aatagaaggt tgagagttat tcggacctga tgaatatttc aattcaccga
    12661 taatgatttc tccgcgaagt gctgttccgg ctgtaataac aactgctttg gcccggtagc
    12721 tcgctcctgt ggacgtcact actccatgac aaacaccatc ttcgacgatc agttcttcga
    12781 cgattccttg tcttagcgtc aaatttggtt ctttttcgat cgtatgtttc atttctgttg
    12841 aataagcatg tttgtctgct tgtgctcgca atgctcggac agctgggcct tttcctgtgt
    12901 tcaacatacg catttggata taggttttat cgatattctt acccatttct ccgcctaacg
    12961 catcgatttc tcgaacgaca acgcctttag ctggtccgcc tacagaagga ttacatggca
    13021 tgaatgctac catgtccaaa tttattgtca gtaataatgt ttttgatccc atacgtgcag
    13081 cagcaagtgc agcttctgaa ccggcgtgtc ctgcgccaac aacgatcacg tcatacgcat
    13141 ctgcttcata atgttccaat cgtttgttcc tccttttatt ttcctaaaca aaattgactg
    13201 aataattgag tgattagctc atcttgtaca ctgtcgccga caacttctcc tagatagtcc
    13261 cagcaacgcg tcatatctat ttgaacgaga tccacaggca ttcctgaatc aatacctgca
    13321 atgacttctt caagtgagag ggaagctttt tctaacaacg cgatatggcg ggtattagaa
    13381 acataagtag cgtctctttc tcctgtttct ccgctgaaga ataaatcaga aatagctgat
    13441 tccaaacgat ctagtccgtc gtttttagct acggaaattg aaaaaactgg ttcattttcg
    13501 atcaatttct tcaatttttc ttgttccagc tgtgctggca aatctgtttt atttagaaga
    13561 atgatccgtt tcaatccact tgtcgcttcc aataattgtt catcttctgc tgtcagtggt
    13621 tcgctttggt ttagaacaag taagatcaag tctgcttctg ctaatgcttt tcggcttctt
    13681 tcgaccccga tccgttccac cacatcttct gtttcccgaa ttccagctgt atcgattagc
    13741 ttgagcggta ccccccgaac attgacatac tcttcgatta catctcgagt cgttcctgca
    13801 atatccgtaa cgattgcttt ttcttctctt aacaaatggt ttaacaaact tgatttgccg
    13861 acatttggcc ggccgatgat tgccgtactt aacccttccc gcagaacctt cccttgcttg
    13921 gatgtagcaa gtagcgcttg gattctttgt tggatcatct gcgctttttc aagcaataac
    13981 ttcgtagtca attcttcgac atcatcatat tctggataat caatatttac ttctacttgt
    14041 gctaatgtct ctaaaatctc ttgtctcaat gagcggatca aagcagacag attgccgtct
    14101 aactgattaa gagctagtcc cattgctttg tctgtttttg cccgaatcaa atccatcaca
    14161 gcttctgcct gtgaaaggtc aacacgacca ttcaagaatg ctcgttttgt gaattcgcct
    14221 ggctcagcta atcttgcacc ttctcttaac aatagctgca aaatttgatt gactacaacg
    14281 atcccaccgt gacaattgat ttctactaca tcttcccgcg taaatgtctt tggtgctctc
    14341 atcacggaga ccatgacttc atcgacaagc tgattgctct tgggatcaac gatatgtcca
    14401 taatggatcg tatgacttgg tacttctgat aatcgtttgt ttccagattg ataaacttta
    14461 tctgctagtt gcacagcttg atcgccactt aaacgaacga tactgattgc accttctcca
    14521 ggtggggtag aaattgccgc aatcgtatca aattctaaag taatttcagc cattttttca
    14581 cttccttttt cattttgcgc acaaaaaaag tgcccactcc accctttatg cacaataaac
    14641 ggtgaacttc gcactttgac tgcttgttct tgattaaatt ctggtcatag attatcacac
    14701 ttgctataag gaatcaagtg tttaaaacaa ttagatgaag ttgagcctgt tctgcttcca
    14761 ttaccttata tttaattgtc cttggatgtc tctttgcgtt ctttggttta ttcgaagaga
    14821 agatactgta taaacaaggc tttatctttc ttaaaaaaaa cggtacacta aatggtagcg
    14881 ctatctaaac tatacacttt tgaaaggaga aaatatgtga gcccgttcag atctgctgca
    14941 ttggaagaag aaagtgaccc tcggatcaac gatgtcgttt tatctacagg gaatatcaac
    15001 cagaaatata tcgtccgcga tatcgttttt gttgtggaat gcttagaagc tgatttgttt
    15061 gacccatctt ttcccaaaga tgaccttttc ttaacaacga agaggaaatt aaagacgaag
    15121 gtacttgaat atggtgcgaa tgcagtaatc aactgtcatt ttgagtatca gcaaaaaaaa
    15181 cgcgacggaa agtattttct ggaacttttt gcttatggaa cagtgatcca atttatccaa
    15241 acaacgattg gttaaatgaa acagacaaaa aaagcggatc aaaactgaag acagtttcga
    15301 tccgcttttt tctatcaaag ttagtgacta cttcatcgta tactctgata agaatgattt
    15361 ctagtaaaat atacttggtt tgaaaaactt tgtcaacgtg atttattcaa aaagtaatgg
    15421 aatcaataat attttttagc aggttctact actaaataac gataaggttc ctcaccttcc
    15481 gaatgtgttt ttacatgttc atctttgctt aatgcagcat ggatttgttt acgttcaaat
    15541 gcaggcattg gttctaagaa aacaggctgc cctgtccgtt ttactttttc agccgtgcga
    15601 tcagctaaac gctgaatgat ctcttggcgc ttttcacggt aattgcctac attgactact
    15661 acagacagct tatttcttgc taaacgatgg atgaagactt gtgccagata ttgcaatgca
    15721 ttcagtgttt ttccatgttt cccaatcagt atgccttgct tttgtgtttc taaatggaaa
    15781 acaaccatat tatttgattc tctttccatt ttgacaagag ctggagcatc aagctgtttt
    15841 gaaatttcag taagataaac tgccaattcc gttaaagctg cttcatcacc tagctcactt
    15901 acttcttctg ttttttcttc aagaatcgtt tcgttgacta cctcgatctc agctgttggt
    15961 tcttcttcca aaatttcttc tacagtctgt tctactgttt cagaaacttt ttcgctgata
    16021 cttggctgga tcgaaagccg tgcattcttt cgacccattc ctaggaaacc tttttttcct
    16081 tcttccagta tttcgatttc gacttggtct ttggccaaac ccaaatcagc cagacctagt
    16141 tggacagctt cgtctattgt tgcagcttca taaatcggca ttagcattac ctcccctatt
    16201 atttccgttt tttcttagga ttcattgctt tcttaagagc acgttctctt tcgcggattc
    16261 tccgtgcttc ttcttcacgc tcttttcgaa ttttgaaagg gttgttgatc agcagcgttt
    16321 gtacaacttg gaaggcattg gaaactaccc agtataatga aagaccgctg gctaaattga
    16381 cacccatcac taaaatcatt acaggcatca cgaaattcat gatcttcatt gatgcattcg
    16441 attcaatttg actcatgcta gataaatacg tacttgcaaa agtaaagatt gctgctaaaa
    16501 tcggcaaaat aaatgttgga tccgcgttcc ctaagttcaa ccataagaaa tgcccttgtt
    16561 tcaactctgg tacacgtgaa atagattgcc ataacgccat caagattggc atttgtacta
    16621 ataaaggaag acaaccagca tatggattga cgccattttc tgcatataaa cgctgctgct
    16681 cttctcttaa cttactttgt gtttccggat ctcttgaact atattgttgc tgcagtgctt
    16741 ttaatttcgg ttgtaattct tgcgttttac gcatgctctt tgtttggaaa tgcatcaacg
    16801 gcaataggat cacgcggatg atcagtgtaa ataaaatgat ccctatccct gcattaccaa
    16861 atgacaacgc cttgatcgcc tcagcaaagt aataaacaat ataacgatcc caaattcctg
    16921 tactctgtgc gctgacttcc ccagttccgc aaccggataa gacgatgacc aaactaacga
    16981 taccggccat caaaagtaaa cgtttgtatt tcttcactta ctcttttcct ctctgtcgat
    17041 tatttttgca agttttaaga cgtggactaa attggagcga acctcatcat aagtcaaccc
    17101 cttcacactt ggtctagcaa tcacaataaa atccaactcc ggatcaattt cttcttttat
    17161 agaataaact gctgctcgga tttttcgttt gacttcattg cggtttaccg cgttgccgac
    17221 cttttttcct acagataaac caactcgaaa atgaggctgt cccggttttt ccaaacggta
    17281 aacgacgaat ttgcggttag caaaagaatc tcccgtatgg aacacggctt gaaactctcg
    17341 ttcttttttt actcgatacg cttttctcat tagttttctt tccttttcta tctctcactt
    17401 acgctcataa tcataccata attacgattg tcctttcatc atttccctaa aaaagagtat
    17461 aaaaaaaacc actgaaaccc cagtggccta agctgaaaga acttttcttc ctttgcgacg
    17521 acggctagct aacacacgac gtccattttt agtactcata cgtttacgga atccgtgaac
    17581 tttttgacgt ttacgtttat ttggttggta tgttcttttc atatattcca cctccaaaat
    17641 gttcttatct attttataaa cacagacata ctttgatagt ataacgacaa agtagtgatt
    17701 ttgtcaatat aaaatagaag atcgattgtt aaaacttatc cacaagatta tttttttcta
    17761 tgggaatcct atgtggaaaa agttatgcac agggattatc cacagataat aaaaggctgg
    17821 ttttacacag aaaacacacg tgtaaaaaaa gtaatccaca atcaggcgat ttcctgtgta
    17881 ttccgtctcc taactattgg tatatctgta ttttttatcc acagacgcaa gaggaaatct
    17941 attggttttt acgagttttc cacctgtgca ttagtggtgt ggaaaacttt ttttgacaac
    18001 tgtgtttttc tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt
    18061 ttcttcggtt tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact
    18121 ttttaatgaa ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa
    18181 gcaacatacc aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac
    18241 cctcgaaccc ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa
    18301 tattgggaaa aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat
    18361 gagattatcc ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa
    18421 aaaaatccaa aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat
    18481 acctttgata cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt
    18541 gtagcagaag atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt
    18601 ggtaagactc acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc
    18661 aaagtaaaat atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac
    18721 aaaacagcag aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac
    18781 attcaatttt ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc
    18841 ttgtacaatg aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg
    18901 aaactgcaag aacgccttgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg
    18961 ccggatcttg aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag
    19021 atcccagatg acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta
    19081 gaaggtgcgc ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact
    19141 agtctagctg cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca
    19201 atcctgcaga tccaagaaga agtggcaaag tattatcaca tccaattgaa agatctcaaa
    19261 ggaaagaaac gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt
    19321 gagctcacag ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca
    19381 acggttatcc atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa
    19441 aatgaagtat cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga
    19501 aaatagcatg tggataaaag aaaaaaacgt tcaaaagtta tacacacttt ttccacagct
    19561 gttttttcgt ttctctctta cgttttttga gttttccaca gaatcaacat gccctattac
    19621 ttttattatt ctttttaact aataataaat aaacaaacat tcaaaggaga tacatcatga
    19681 aagttacttt aaaccgagct agctttatgc aggaattgca aactgttcaa cgagctattt
    19741 caagcaaaac cacgatccct attttgacag gtgtaaaaat cacactgaca caagaaggtt
    19801 tgactttgac ggggagcaac gctgatatat caattgaaac ttttttgtct gttgaaaacg
    19861 aaaaagcaaa tatgcaaatc gaatctactg gttccattgt tttacaagca cgtttcttta
    19921 gcgaaatcat tcggagactt cctgaagaaa catttacttt agaagtttta gaaaataaac
    19981 aagtagcgat cacttctgga aaagcgaatt ttatcgtaaa tggattagat gcagataact
    20041 atcctcatct tcctgttgtc gaaagccata accagatgaa attacctgta cacgtattga
    20101 ctaaactaat caacgaaaca gtttttgctg tctcacaaca tgagagtcgt ccaatcttga
    20161 caggtgtgca tttcatttta tctgataatt ctttattagc tgtagctact gattctcacc
    20221 gtctaagtca acgcgtgatt ccagtagaac aagcggctga tcattttgat attgttattc
    20281 ctggaaaaag tttgatcgaa ttatctcgct cattaacaaa tgaagaagaa atcgtcgaaa
    20341 tcagcattat ggaaaaccaa gtgttgttca aaacagaaac gatgtatttc tattcccgtt
    20401 tgctagaagg aaactatcca gataccaatc gtttgattcc ttcaagtttt aatacagagg
    20461 ttgaattttc tgttcctagc tttttagcgg cgatcgaacg ggcttcttta ctttctcatg
    20521 aaggccgtaa caacatcgtt cgtttatcta ttcgtccaga tgcggttgtt ttatacggaa
    20581 attcgccaga aatcgggaaa gtagaagaaa gcttgagtta tacagcaagc agtggcgatc
    20641 cattagatat ttctttcaat ccggattata tgaaagcagc gttgcgtgca tttggagata
    20701 tgagcatcaa agtgaaattt atctctgcta ttcgtccatt tacattggaa ccaacggaag
    20761 atggcgttca atttattcag ctgattactc ctgtacgtac aaattaacaa tgattttcaa
    20821 tgttcttgaa aatttaattg aaaagaaata gaaaaaataa aattttaaac agaaggccct
    20881 aaaatctctt ttaaggcttt ctgtttattt ttgtttaaat cacctttttt agattaaatc
    20941 gtcttatatg gaaaaatata tggataaatt tgttttattg cgaaaaaaaa ggtataatag
    21001 agtatacttt taataatgaa aatgagggat gggcattgaa acaacaagtc attctaaata
    21061 cggaattcat gacgttgggc caaatgttga aagaagtcac agttatcggt agtggcggac
    21121 aagcaaaatg gtatttggca gaaaacactg tgttagtcga tggcgaacca gaaaatcgtc
    21181 gtggacgcaa actttatccc ggcatgatgg ttgaagttcc agaagtagga acttttttta
    21241 tggtaaaaaa aggaaacgac gataatgagg ctgaataagc tgtatttaaa aaattatcga
    21301 aattacgaag agctgaacat cgaattttcc aagaacctcg tcatcttttt aggagaaaat
    21361 gcgcaaggaa aaacgaatct tttggaaagt atctacgtat tagctatgac gcgaagtcat
    21421 cggacaagta gtgagcagga attgattggc tggactgacg atcaggcgat gattcaagga
    21481 gagatcacaa aaggttcatc cactcttcca ttagaaatct tattatctaa aaaaggccga
    21541 aaaaccaaga tcaaccatat cgaacagaaa aagttaagta cttacgtggg acaaatgaat
    21601 gtcattttat ttgcaccaga agatctttct ttagtaaaag gcagcccgca gaccaggcgg
    21661 aagtttttag atatggaatt aggccagatc aatccaatct atctttatga tcttgtccaa
    21721 taccaagcaa ttttgaaaca aagaaaccag tacttgaagc aattgaatga aaaaaaacaa
    21781 actgatttgc tttatttgga tattttgtcc gaacaactag cagcgtttgg cagcaaagta
    21841 ttgaaagcaa gagcacagtt cgtcaagcga ttggagtttt gggctaattc tttgcatcag
    21901 caaatcaccc atcaaaaaga gcagctggaa atcgagtatc tcaccgcagt cgattcttta
    21961 gaaacacata cgcaagagca aatccaagaa caatttctcg cacttttgaa ccaaaataaa
    22021 aagaaagacc tttttcgggg aacaacgaca gttggtcctc atcgtgacga tctttccttt
    22081 tttattaatc agaaaaatgt gcagacttat ggatcgcaag gacagcaaag aacaactgca
    22141 ctaagtgtca aattagcaga aatcgatttg attaaagaag agacagggga atatccaatc
    22201 cttttgttag atgatgtgat gagcgagctg gatgataatc gtcaactcca tctgctagaa
    22261 acaatcgaag gaaaagttca aacgttctta acaacaacga cacttgacca tgtaaaaaat
    22321 aaaatgactg ttgaacctga gattttttat gttcaacaag ggaaactaaa aggagaagaa
    22381 actgaatgac agaagaaaga agtttagtag aacgcgctaa agagtatgat gccagtcaga
    22441 ttcaagtact tgaaggactt gaagccgttc gtaaacgacc tggcatgtat atcggttcga
    22501 ctagttctga gggtctgcat caccttgttt gggaaattgt cgataattcg atcgatgaag
    22561 tattagcagg ttttgcaacg aaaatccatg tgattattga aaaagacaac agtatcaccg
    22621 ttattgacga cggtcggggg atacctgtag atatccaggc aaaaactgga cgtcctgcag
    22681 ttgaaacggt ctttactgtt ttacatgctg gaggaaaatt cggcggtggt gggtacaaag
    22741 tttctggtgg acttcacgga gtaggatctt ctgttgttaa cgcgctttct acacagcttg
    22801 atgtcaaagt atacaaagaa ggaaatgtgt actatcaaga atatcgtcgt ggagctgtag
    22861 tggacgactt aaagattatc gaacaaacgg atcgccatgg aacaacggtt cactttacgc
    22921 cagaccctga aatttttaca gagacaactg agtttgattt ttcaaaattg gctacccgga
    22981 tccgtgaact tgctttcttg aaccgcggga tgcgtatatc gattgaagac aaacgagaag
    23041 aagaaccagt catcaatgaa taccattatg acggcgggat taaaagttat gtcgaatact
    23101 tgaatgcaaa caaaaccgtg atttttccag aacctgtata tttggaaggt gaacagcaag
    23161 atatcgcagt tgaagtctct atgcaatata cagacggcta ccattccaat atcatgagtt
    23221 ttgccaacaa tatccatacg tatgaaggcg gaacacatga atcaggattc aagactgctt
    23281 taactcgtgt gataaatgat tatgcccgaa aacaaaagct gatgaaagaa aatgacgaca
    23341 acttgaccgg tgaagacgtg cgagaaggat tgacagctgt tatttctatc aagcatcctg
    23401 accctcaatt cgaaggacaa acaaaaacaa aattgggcaa ttccgaagta cggacagtca
    23461 ctgatcggtt atttagcgaa catttcatga aattcttgct ggaaaaccca agtgtgggcc
    23521 gtcaaattgt tgaaaaagga ttactagctt ccagagctcg tctagcagcg aaacgtgcgc
    23581 gtgaagtaac gagacgtaaa ggtgctttag agattagtaa tctgccagga aaattagcgg
    23641 actgctctag caacgatcct gaaaaatgcg aattgttcat cgtcgaagga gattcggccg
    23701 gcggatcagc taagcaaggt cgtaatcgag agtttcaagc catcttgcct attcgtggga
    23761 aaatcttgaa tgttgaaaaa gcaagcatgg acaagatctt ggcaaatgaa gagatccggt
    23821 ctttatttac tgctatgggt acagggtttg gagaagattt tgatgtatcg aaagctcgct
    23881 accacaaatt agttatcatg actgatgccg atgtcgacgg cgcgcatatc cgtacattgc
    23941 tgctgactct tttttatcgt tacatgcgtc ctattgttga agctggatac gtctatattg
    24001 ctcagccacc tttatacggg gtaaaacaag ggaagaatat tacgtatgta caaccaggaa
    24061 aaaatgcaga agaagagttg aaacaagtcg ttgcttcact tcctgcctca ccaaaaccaa
    24121 gcgttcagcg ttataaagga cttggagaaa tggatgatca tcaactatgg gaaacaacta
    24181 tggacccaag caatcggatg atggcccgcg tcagtgtgga cgatgctatt gcagcagatc
    24241 agatatttga aatgttgatg ggggatcgag tagaaccccg tcgtgcattc atcgaggaaa
    24301 atgcacacta tgtgaagaac ttagatattt aatcatgaag gaggaaaaag aatgagtgaa
    24361 gaaatcagag aaaacatcca tgatgtcaat ctaaccagcg aaatgaaaga ctccttcatc
    24421 gattatgcga tgagtgttat tgtcgcacgt gcacttcctg atgtcagaga tggtctgaaa
    24481 cccgttcatc gccgtatttt atacggcatg aacgaattag gtgtaacacc ggataaagcc
    24541 cataaaaaat ctgccagaat cgtcggagat gtcatgggta aataccatcc tcatggagac
    24601 tatgcgatct atgagtcaat ggtacggatg gcacagccat tcagctaccg ttatatgcta
    24661 gtggatggtc atggaaactt tggttctgtt gacggtgacg gtgccgccgc tatgcgttat
    24721 accgaagcac ggatgagcaa aatcgctaca gaaatgctgc gtgatatcaa taagaacact
    24781 gttgatttcc aaagcaacta tgatgataca gaaaaagaac cagttgtact acctgctcgc
    24841 ttcccgaatc ttttagtgaa cggaacaacg gggatcgccg taggtatggc cacgaatatc
    24901 ccacctcata acttgtctga ggtagtagcg gctatcgatt tgctgatgga taatcctgat
    24961 gtgacgacca atgaattgat ggaagtatta cctggaccag attttccaac aggtggtttg
    25021 gttatgggaa aatcaggaat ccgaagagcg tatgaaacag gtaagggttc tattaccgta
    25081 cgtgcaaaag ttgagattac tgagatgcca aatggtaaag aacggatcat cgtgacagaa
    25141 ttgccttata tggtcaataa agcaaaattg atcgaacgga tttctgaact gcacagagac
    25201 aagcgtatcg aagggatcac agatttgcgg gacgaatctt cccgtgaagg tatgcggatc
    25261 gtcattgaca tccgtcgtga cgccagcgca tctgttatct tgaataactt atataaattg
    25321 acatccttgc aaaattcatt tggattcaat atgttggcga tcgaaaaagg tgtgccgaaa
    25381 gttttgagct tgaaacaaat tcttgaaaac tatatcgaac atcagcgcga agtcatcaca
    25441 agacggacag tctttgaaaa agaaaaagca gaagcaaggg cacacatttt agaaggtttg
    25501 cgtattgctt tggatcatat cgatgaaatc attgcgatta tccgcgggtc taaatcagat
    25561 gacgaagcaa aagcgacgat gatcgaacgg ttcgatttat ccgatcgcca gtctcaagct
    25621 attttagata tgcgtttacg ccgattgacc ggtttggaac gggaaaaaat cgaaaatgaa
    25681 taccaagaac tcttaaaact gattgaagat ttaactgaca ttctggcaag accagaacgt
    25741 gttacagaaa tcatcaagaa tgaattagca gacttgaatc aacgatttgg tgataaacga
    25801 cgcacagaat tactagtcgg tgaggtctta agtctggaag atgaagattt aatcgaagaa
    25861 gaagaaatcg ttattacatt gacaaacaat ggttacatca aacgtttggc aaataatgaa
    25921 ttcagagctc aacgaagggg tggacgtggt gtccaaggaa tgggtatcca caatgacgac
    25981 ttcgtcaaaa atctggtttc ttgttctacc catgatacat tgttattctt tacgaataac
    26041 ggaaaagtgt atcgggcaaa aggatacgaa attccagaat acgggcgcac agccaaagga
    26101 ataccaatta tcaatttact aggtatcgat tcgagtgaaa gcatccaggc aatcattgcg
    26161 gtgacgggag aagcagaaga aggccactat ctcttcttta cgacgcgtca aggtacagta
    26221 aaacgtactt ctgtcactgc ttttgctaac atccgaagta atggtctgat cgcaattggt
    26281 ttgaaagaag acgatgagct cgtcaatgtg ctactgacag atggtcaatc taatatcatt
    26341 attggaacac ataacggcta ctctgtcact ttcaaagaag aagcagtccg cgatatggga
    26401 cgtacagctt ccggggttag aggaatacgc ctgcgtgagg aagattatgt cgttggggca
    26461 gccttaatga aggaagatca agaagtatta gtgatcacag aaaaaggtta cggaaaacgg
    26521 acaaaagtat cggaatatcc agtgaaaggc cgcggaggta aagggatcaa aacggcgaac
    26581 atcactgaaa aaaatggtcc actagctgga ttgaccactg tttctggaga tgaagacatc
    26641 atgctgatta cagataaagg tgtcattatc cgtttcaacg tatcgactgt ttctcaaact
    26701 ggacgttcaa cattaggcgt acgtttgatg aagatggaag ctgatacgaa agttgtcacg
    26761 atggcagcag tcgaaccaga agctgcagaa caaacagttg aagaacaagg atcagaagaa
    26821 tagccatcaa gactaattgt gtaagctagc aagaatgacc gattaaaaaa tctggttatt
    26881 cctgctggct tttttgatta gttgaatttc tcttaaaata ttcgaaactg ttcattctgt
    26941 attgcttttg accaaaatag agtgtataat cagagcttgt gagtagtcta attaattaga
    27001 taactctcct tgctcttagg aacctaagag ccgaaagtcc acagggaggt gaatatcaat
    27061 ggaaaacacg aaatatgaaa tcatgtacat tattcgtcca aacattgatg aagaagcaaa
    27121 aactgcttta atcgaacgtt tcgatgcaat cttgaaagat aacggagcag aagttatcga
    27181 atcaaaagat tgggaaaaac gccgtttcgc ttatgaaatg aacggttacc gtgaaggtat
    27241 ctatcacatc gttaaagtat cttctccatc atcagcaaat gctgtcaatg aatttgaccg
    27301 tcttgctaaa atcaacgacg atatcattcg tcacatgatt gttaaagaag aagcttaatt
    27361 gttgtttcat gtgaaacaat agaaaacgat gagaggagat gactattttg attaataatg
    27421 ttgtattagt tggacgtctg acgaaagatc cagatttgcg ttacacagca agtggtacgg
    27481 cagtcgcaac atttactttg gctgtgaatc gtaattttac aaaccaaagc ggcaatcgcg
    27541 aggctgattt tatcaactgt gtgatttggc gcaaatcagc agaaacttta gcaaactatg
    27601 ctcgtaaagg aacactttta ggtgttactg gacgtatcca aactcgttct tatgataatc
    27661 agcaaggaca acgtgtatac gtgacagaag tagtagctga caacttccaa ttgctggaaa
    27721 gtcgttctgc atctgaaaat cgccaacaaa gcggcggatt ccaaagttct ggtcaatcag
    27781 ctggcggatt tggcggaaac aataactcta atcaaacatc tcaatcatcc aacggtatgc
    27841 ctgatttcga ccgtgatact tctgacccat tcggctcaag ttcaacgatc gacatttcag
    27901 acgatgattt accattctaa acgaatttga taggagggaa atagaatggc acaacaaaga
    27961 agaggcggac gtaaacgtcg taaagtagac tacattgctg ctaaccatat tgaatatatc
    28021 gattataaag atatcgaatt actaaaaaga tttatttcag aacgcggtaa aatcttgcca
    28081 cgtcgtgtaa ctggtacagg cgctaaaaac caacgtaaaa ttactgttgc aatcaaacgc
    28141 gctcgtatca tgggcttgct acctttcgtt agcgaagact aataaatcct aggaattgaa
    28201 tgactgatcc ttttgatcag ctattcaatt cctttttttc tgtaaaaata acctctattc
    28261 gtttagtcgc tcgaatgacg aaagttatgg taaaatcaaa ttagtcatat atgggaggaa
    28321 aattatggaa aataaaaaca atcatttatc cccgaaacct ctcttcctta tcctgtttat
    28381 attaatcgaa gtattggtag tcttaatttt accatttcgt tttgttagcg cttcggtcat
    28441 tctattgatg aatttatggg gtgttcgcta ttggttgaaa atgaaacgtt ggcttatttt
    28501 aaataaacgt gaaaaaatcc agcaggcttc gactgtagct gaaaccaatc tagcttatgt
    28561 ctcagaagtc atgcctgtag gagtgattgc ttatcagcca tcaagccaaa aaatcgaatg
    28621 gctcaatcca ttagcattga aaatcaaaga tcaaagtgga ttaacagagg aagaattgct
    28681 agatactttt tttactgcaa aagaaaaaaa gaaaaaccaa gtctatctag cagataaaac
    28741 atttcagttt gatatggata cagtaaaggg tgttgtttat tttaatgata ttacccaaaa
    28801 ccagttgtta aaactccatc agacgaacag tcagccggtg attggtattt tatctattga
    28861 taactatgca gatgccattg ataaattgga tgacaaagag atttcttatt tgaactcttt
    28921 cgtcactaca ctagtttctg actggatgaa tgaataccat gtctttttca aaagaatcaa
    28981 cgcagaacgg tttttcttcg tagcacagat gatcgatctg aaaaagatga tggataaaaa
    29041 atttgatttg ctggatcgct ttcgagcaga agctgaaaaa cagagctcgc ctatcaccct
    29101 tagtatgggg attgcttatg gctcaagaac cctcgatgaa atcggtggag aagctcagaa
    29161 taatttagat gttgctttag tccgtggagg cgaccaagtt gtcgtgaaag aggtaaaaga
    29221 aggagcaaaa cctgtttatt atggggggaa atctgctaag aatgtcaaac gcacgcgtgt
    29281 tcgttctcga gcgatgagta ccgctttgcg tcggattttt gatgaaacag gcgatgtgtt
    29341 tatcatgggg cacaagtttc cggatatgga tgctttaggc tctgcttttg gtgtcagttg
    29401 tctggcacgc ttcaaccaga aaaaagctta catcgttatc aacgagaacg agatcattcc
    29461 agatgtagaa cggtgtcttg aagaaatcca taagcatcct gaattagaaa tgcaactaat
    29521 ctctccagaa cgtgccatag agctgaaaaa agaagaggac ctcctcgtga tggtggatta
    29581 ccataaacct tctctatcga tctctcaggg cctatatgag caatttgata agattgtgat
    29641 cattgatcac catcgtcgtg gggaagagtt tccaagtaaa cctctgctga cttatatcga
    29701 atcttcagct tcttcagctt cagagctagt ttctgagctg atccaatacc aaagcagcaa
    29761 aacaaatcgc ttagatcgtt ttgaggccac attattgctt gcagggatgg ttgtagatac
    29821 aaatagtttc aatgtccgta caactgcacg aacttttgac gttgccagct atttaaggtc
    29881 ttgtggagca gacgcatcct tagtacaata tttattgagc tcggatttag cgacatactt
    29941 ggagatcagc aatttgattg ctggaagcga ctatgtgact tctgatattg ttgtggcggc
    30001 agggacagac gaaaaagagt ataatagtgt tatagcagca aaaacagcag atacattgct
    30061 ttcgatgatc aatatcaatg cagctttcgt cattacaaag cggacagacg gcttgattgg
    30121 gatcagtgcc agaagtaccg gcacgatcaa cgtccaaatc atcatggaat ccttaggtgg
    30181 cggaggacac ttcacaaacg cagctactca actggaaaat acgacagttg aggaaacaaa
    30241 acagaagcta tttgctgcaa tcaaagaaaa tatgaatcaa atatatgata aggagtaatc
    30301 caaatgaaag taattttttt acaagatgta aaaggaaaag gtaaaaaagg ggacatcaaa
    30361 gaagttccag caggttatgc acaaaattat ctaatcaaaa ataacttggc aaaagaagca
    30421 acgaaaagca gtatcagtca gttagcagga caaaagaaag cacaagaaaa acacgaagct
    30481 gaaatcttgg aagaagctaa aaagttaaaa gaattcttgg aaaaagaaga tactgtagtc
    30541 gaattgaagg cgaaagcagg ggaagacagc cgtttgtttg gttcgatccc ttcaaaacaa
    30601 attgctgaag ggttaaacaa acaatacaaa atcaaattag ataaacgaaa aatcgaatta
    30661 gaaaatccga tccgttcact aggctacaca aacgtacctg ttaagcttca tcatgaggtg
    30721 acggctacga tcaaagttca tgtcgtacaa gaataacaaa tcttgatagc gatgttcagc
    30781 tggtaagtat aaccagaagg aaaaaagaca aaccgaaatt tatctgagaa aacagctgca
    30841 gcaatgaaag caaaagctgc aaatcgattt cagattgatt tcggtttctt ttttgggaac
    30901 agcaaaatag cataaagaaa acgagtataa taaaagagaa ctattttttt attacacttt
    30961 cggtacgaat gatgactttt ctctcatttt cttgtaaaat gaagcaattg aagacaagga
    31021 gcgatacgta tgaatgaagt gtggcaggat cgaatcccac cgcaaaatat tgaagctgaa
    31081 caagcggttt taggctctgt ttttcttgac gcagaaacga tcattgatgc catggagtac
    31141 atagagccaa aagactttta tcgtcgtggc catcaaatta tttttgagac aatgatcgag
    31201 ttaaatgatc gtaatgaagc gattgacgtg gttactgtca aagaccggct ggaacaagcc
    31261 aatttgttag aagatgccgg tggtttaagt tatctctcag acttggcatt agcagttccg
    31321 acagcagcaa atatcgtcta ctatgcgaaa atcgttgaag aaaaatcatt gctgcgtaca
    31381 ttgatccaga cagcaacagg aatcgttacg aaaggattcg agcaaggaga agaagttcaa
    31441 acaattttag atgatgccga acgcagtatc ttggaggttt cggaaaaaag aaatcgaagc
    31501 ggctttttat ctattgctga agtactgaac tccacgattg aaaatatcga tcgactagct
    31561 caaaacaatg aagaaatcac aggtctacca actggctatc aaggtctgga taaaatgaca
    31621 gcaggtttcc agcccgaaga actaatcatt cttgctgcac gtccagcggt aggtaaaaca
    31681 gcgtttgcat tgaatattgc tcaaaatgta ggaacaaaaa cagatcgttc tgttgcgata
    31741 tttagtttgg aaatgggggc agaatctctt gtaaatcgga tgctttgtgc agaaggttcg
    31801 attgaggcca gccatttacg tacaggtcag ctttcggaag aagaatggtc gaatctgatc
    31861 gttgcgatgg ggagtctttc tcgagcaaat atttttatcg atgatacacc agggatcaaa
    31921 atctctgaaa tccgtgcgaa atgtcggaaa ctagcgcaag aaaaaggcaa tctaggcttg
    31981 atattgatcg attatttgca gttgattgaa ggtaacggta aagagaaccg tcaacaagaa
    32041 gtatcagata tctctcgcca actgaaaaaa ctggcgaaag agctgaaagt acctgtcatt
    32101 gctctttcac agttatctcg tggagtagaa cagcgacaag acaaacgacc agtgctaagt
    32161 gatattcgtg aatccggatc aattgaacaa gatgcggaca tcgtagcctt cttgtatcga
    32221 gatgattatt atgaaagaga aggcggagat gacgatgatg gaaacgagcc aaaaaatgac
    32281 aacgtcatcg aagtcatcat cgagaagaac cgaagcggtg cacgaggaac agttgaacta
    32341 ctgtttatca aagaatacaa taagttttcg tcgctttcgc caagagaaga attttaaaga
    32401 agaggttgtg agccttgagc aatcccgaga gctctcgtaa gaagatgcaa ggagtaaccg
    32461 caggaacgac tagagtactt aatttaaaca gatctccaaa gggattgtga caaaagtctt
    32521 gtcacagtcc ctttttccga ataaacggtg ttcaatcagc tgacccttgg tattagctca
    32581 gcaaacgtca aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa
    32641 cgctgatctt acgacctctt ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa
    32701 gtcacaatat catcaaacat gagaagcagt tttttgaaat tgctccttgt gactcagagt
    32761 taaacggcag gctcacaacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
    32821 tacgcccaat caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
    32881 atgcaggaca gctttttcac aacctctgat tcacggtgtt ccaaaagtag cttcttcgaa
    32941 aaataagtcg aaatttcaca aaaatttgag aagcaatttt cgtacattcc ttcttatttc
    33001 ttgaagctga ccacttctgt tacaacctct tttttaataa ccattctaaa aagataaagt
    33061 tcgtttttta agcgttttta taaaataaaa ataaaaatca ttcgatttta tttgaaatat
    33121 gtatctatta ttgatacaat ggttcaggat taaataatat agaacgaggt gttcacatgt
    33181 catctgtagt agtagtaggt acgcaatggg gcgatgaagg gaaaggaaaa atcacagact
    33241 ttctcagtga aaatgctgaa gtgattgcac ggtaccaagg tggcgacaat gcagggcaca
    33301 caattaagtt taacggtgtg acttataaac tgcatttgat tccttctggg attttttata
    33361 aagacaagat cagtgtgatc ggaaacggcg ttgtagtcaa tcctaaatca ttggttaaag
    33421 aattagctta tttacatgaa aaaggtgtag aaacaagtaa tttgcgtatt tctgaccgcg
    33481 cgcacgtaat cttgccttat catatcaaat tggaccaatt acaagaagat gctaagggcg
    33541 aaaataagat tggtacaacg attaaaggaa tcggacctgc ctatatggat aaagcagctc
    33601 gtgtaggtat ccgtattgca gacttattag ataaagaaat ttttgaagaa cgtcttcgtt
    33661 tgaatctaga tgacaaaaac cgtcaatttg taaaaatgtt tgattcagaa ccacttgatt
    33721 ttgacgatat ttttgaagaa tattatgaat acggacaaca aatcaaacaa tacgtcacag
    33781 atacttccgt tatcttgaat gatgctttgg atgcaggcaa acgcgtgttg tttgaaggcg
    33841 cacaaggtgt aatgctggat atcgaccagg gaacttatcc ttttgttact tcttctaacc
    33901 cagtagccgg tggggtaaca atcggaagcg gtgtagggcc ttcaaaaatc aataaagttg
    33961 tcggcgtctg caaagcctat acgtcacgtg tcggtgatgg accatttcca actgaattat
    34021 ttgatgaaac aggagaacaa atccgcgaag taggacgaga atacggtacg acaactggtc
    34081 gtccgcgtcg tgtaggctgg tttgattctg tagttatgcg ccattctaaa cgagtttcag
    34141 gtattaccaa cctatcttta aactcgatcg atgtattaag tggattggaa acagtaaaaa
    34201 tttgtacggc ttatgaatta gatggcgaac taatctacca ctatccagca agcttgaaag
    34261 agttgaaccg ctgcaaacca atctatgaag aattgccagg ctggtcagaa gacattacag
    34321 gatgcaaaac acttgcagaa ctgccagaaa acgcacgtaa ctatgtacgt cgtatctcag
    34381 agcttgttgg tgtacgtatc tcaacatttt ctgtcggacc tgatagaaca caaacaaaca
    34441 tcttagaaag cgtctgggcg caaatctaag gataaaaacg acgactgaat caggacgttc
    34501 ttaccagagc gagtcgaagt gtttatctat acgataataa taacttgaac actgtttatt
    34561 cagaataagg gactgtgaca aaaaccgtgt cacagtccct ttttccgaac aaacggtgtt
    34621 caaaagcttg catctacggc actaagccca atcagatgaa aaatatggag tgctattttt
    34681 atctgattgt tcttactgct tgatgcagag cagctttttc acaacctcct acatggtgtt
    34741 caaaatatta ttcgcctttt gcttctttca ctaattttgc gaatgcttgt tgttggaatt
    34801 cagcagctaa ggctttcgta cgttgttctt gctctgcttt gctcattttt ggattttcag
    34861 tcattgcttt taccataccg tcttgaacat aagcttttgc ttcactttcc atcgtacttt
    34921 gctgtttctt cactaattca gctaacttgg ctgcttggtc cgtacttaat tctacccatg
    34981 tctctgggat tttgaatgta ttcactcggc tgtcatattc gtgattgtta tcagcaatat
    35041 tgaaccagaa tttatacatg tcgtttttgt attcccaata agtagttttc gtcacttttt
    35101 cagcatcgcc gtctgtcttt tctactttat ttgtttcgtt atccgtacca gttgcttttg
    35161 gtttttcttg tttttcattt gttcggtaaa gatacacttt ttcttttcca ttccctaaag
    35221 ctttatacaa aagcatatct gctccttggg aatcagcact ggaaacaatt ttttctgttt
    35281 ttgtttccgt tactttttcc attccaaagt gattggacaa attagcgacg atcaaactta
    35341 gtgaagcgac gaagattagt ccgaaaatca aagataaaaa ggtttgccat gtttttttag
    35401 cgaacacgtt cgttaaagca aaagcaagca agctgacaat caaaactcca ataatcatgc
    35461 attatccccc gctttcatta gaacaggttc ttttttcttc aataaaaagg caataataaa
    35521 gccaagaatc ccaaagatga tcgctacgat aaacgcggca tggtaacctg acagtgtggc
    35581 attgattgcc tgatctttat aagctaatgg cgtattatcc aatacagatt tgcttgggat
    35641 atttgctttt gtcacattcg tcaacacact gatcaaaata gctgtaccaa tcgagcttgc
    35701 gatttgacgg aatgtattat ttaccgctgt cccatgactg atcaagttat ttggcaatgc
    35761 attcatacca gaagtcgtta ccggcatcat gaccatggag atcccaaaca tacggatcgc
    35821 ataaaggatc acgatatata aaactggagt cgtttttgtc aagaaagcaa atggtgcagt
    35881 tgccgctgtc aaaatgaaca tccctgtgat ggctaaacgt ttcgcgccgt atttatcgaa
    35941 aatcgcaccc gtcacaggca tcatagcccc catgatcaat gcacccggca acaacatcaa
    36001 gccagaatgg aatgctgatt ctccacgaat attttgaata tacaatggta cgaccatttc
    36061 tgcccctacc atcgccatat ttgttacacc agctaaaaca gctgcaatcg tgaatgtaga
    36121 tgatttgaat acacgaagtt ccaagaatgg atgttccaaa tgtaattgac gccatgagaa
    36181 caaggcgatg aaaatcacac caacaactaa gaaaccaatt actttcaagc ttccccagcc
    36241 gtcatctcct acactagaaa atccgtaaag taaactaccg aatccaatcg ttgataaaat
    36301 agcggataaa atatcaatgc taggatttga caatggaata acacttttca ttaggaagaa
    36361 tgctaaaacc aagacaagaa caacgattgg cagtaccata ccaaacaagt cacgccaatg
    36421 gtagttatca atgatataac cagataatgt agggcctaaa gcaggagcaa gtccaataac
    36481 gatccctgac atccccattg ctgatcctct tttttctggc gggaaaatag atagcatgat
    36541 attttgcaat aatggcatcg atactcccac accgcaagct tggatcaaac gaccgattag
    36601 taatgtacta aaattaggtg ctgtaaagca agtaatcgtt ccgatcaaaa agatcgtcat
    36661 ggcagaaaga tataaatttc ttgaactgaa tttattgatc agccatgctg ttattgggat
    36721 catgatcccg ttgactagca agaaaccagt cgttaaccat tgcacatcag acgctgaaat
    36781 atcgaaagct ttcatcaatg taggaaaagc tgtcgtcaat aaggtctgat tcaaaacggt
    36841 acaaaatgta ccaattaata agacaacaac taaaagtgtc cgattatatg gttttccgta
    36901 aatatctacg ggttggttgt tactcatatt tctccttctt tctgaatttt ttctgaaact
    36961 actagaatac tttagtccta aattcgctat ttgtcaacct acttgacaca taagttaata
    37021 cggatataat taagggaagc aagaggacaa aaggagtgac aaaaatggtt ggggcttccc
    37081 ttaaacgttt gttggaaaat gatcaattgg ttgttggcat cagtgaatta agtgatatcg
    37141 taggtgtgtc tccacgtcaa ttaaggtatt gggaacaaaa gggattcatt cgttcgatcg
    37201 aagctgatgc gaattgtcct agaaaatatc gtttacctac agtcataaaa gtagaactga
    37261 tcaaaaaata tttagatgac ggatttactt tgtcaaaagc tgcagaaaag gcagaaatcc
    37321 gccaaaagaa aatgcaccat gtccgtaaag ttttttcaaa agcaatcaaa gatatcgaat
    37381 tgatcgacga acgctatacg attttacaaa tcggtaactt caaagaagag aatgctaaga
    37441 tgtacatcat tcatgacaac gagacagacg aattaactta caagctcctt cctgtcgatg
    37501 aaaaagctga ctacgaacag ttgttaaaag aactctaaac gccactaatc tcctaagtac
    37561 caagcttttt aataagtaga tgctacataa atacttgaaa aaaggcctgt ttgaacataa
    37621 gaatatccga acggtgatct gcgtttcttt catatagaaa ggaattctcc atcactgttc
    37681 ggatattttt ttattttttt cggttacttt cttctaatac atctgttagc caatttgcat
    37741 attctgtttc ccgattgatc gcatgcgcta aaatcaggta gtgtccgtaa ttttcatgtt
    37801 tttctttttc agtaggaaag atcgtttttt ttcgctctaa tagatgaagt cgtttttctt
    37861 catgaaggtc acgctgctgc tgaataattt ctactaatgc cggatcattc acgtctttga
    37921 cgaaatacaa tttcagaaca aattcgtctc gattaaccgg tagttcattg gtgggtgtat
    37981 gaatccactc tgccaattgt tccttccctt cttctgttaa ttcataaact ttcttttcca
    38041 atttttcacc tgtgatctct acttgatgct gaatgattcc ttgctcttcc atttttgcaa
    38101 gttcaggata aatctgacta tgttttgctt gccaaaattc tcctatttca tttttgaacg
    38161 attgattcaa ttcatatcct gtcatcttct tcttacttaa taaccctaac aaaatatatc
    38221 gaagtgtatt ttttcttgcc atcagttttc cctccttgac aaatctttaa atccattcta
    38281 tcataaatga gtaatcgaaa acatgtagtt tattacatgt tttaatttat aaggagatgc
    38341 ttaaacatga aagaatttaa agaattggaa gattttattg gaactcactt tatctatacg
    38401 tatgacaatg gttgggaata tgaatggtat gccaaaaatg accatactgt tgattaccgt
    38461 atccatggcg gaatggtcaa aggccgttgg gtaaaagacc aagaagctga tatcgttaaa
    38521 ttgacggacg gcgtattcaa aatcacttgg acagagccaa cagggacaga cgtggcattg
    38581 gactttatgc caaatgaaaa gaaacttcac ggaatgatct ttttccctaa atgggttgaa
    38641 gaacatccag aaatcactgt tactttccaa aacgaacata tcgcagaaat ggaagcagct
    38701 cgtgaaaaat atgaaactta tccaaaatta cttgttcctg aatttgctac gatcacttat
    38761 atgggtgatg caggtataga caatgaagat gttattagcg aagaaccata cgatggaatg
    38821 ccagatgata tccgtagcgg aaaatacttc gatgaaaatt ataaacgagt aaaataaggt
    38881 tgtgaaagag aggttgtgaa atcagcgttc tgacctgagt attagagaaa gaaacggaaa
    38941 aatagttcct catgttttgc cgttttttga gctaataccg agggtcagct gattgaacac
    39001 cgtttatcaa gaggttgtga aatcagcgtt atgctttgag taataagaac gctagttaga
    39061 aaatagcttt tcatattttc taggctagtg ggcttattac cgagaagcag ccgtttgaac
    39121 accgtttatc aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaatgg
    39181 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
    39241 caccgtttat caaagattgt aacaactgca tattcacaca gttaaattag tgacttcaaa
    39301 atgaaataat gctcccagca aaaaattagg ctgggagcat tatttcgtta ttctgttcat
    39361 caaaaatcag cgttttgatt ccatttcatc acaagttcct cctcatttac tgcttcatct
    39421 ttttgctgtg tttgaattgt aaaaccttgt gaaagataga aatgaatagc tgattgattt
    39481 ttttgataga ctgtcaaagt cagtgtttta ttggtttctt ttattttttt cattagttgc
    39541 gtaccgattc tttgttgccg gtattccctt ttcacaaaga ttcctgcgat aaactgatca
    39601 atcaccccag caaaaccaat aatttcttga tctatttctg ctaagtaaat cactgcatct
    39661 ggtaaagctt tcctaacaga ttctacttga ttctcccaat aagttgatgg aatgaattca
    39721 tgagaatcca agttagcatg cagccaaatc gatagaacag attccagttc tttggcagac
    39781 aattttgttg ttgtgcggat cattttttct ctccttactt attcattgat tttaaaataa
    39841 aaaccatttt tttgaagctc ttttacaagt tttcgcccct caaaaaagta tatcttctct
    39901 tgttttactt ttctaatacc aaaagaaaca gcaactttag gatcatcagc tgaaacgatt
    39961 cgataagttc caccaggcaa ttgatccagc ttgactttcc catgatggtc gctttgtact
    40021 gaaaacggca caccatttct agtaagtgtt tttttcttat gtttcaaaat gaatgaacta
    40081 ccatttaccg gtttccctga cgaatcacta ataataaatg aaaaatcaag cactttttta
    40141 cttaatctat actgatagat tagtcgtccg ataaacacta acagtatgac tacagctatt
    40201 atcgttcctg caagaataaa ccattgccgc atggttcttt cctgatttcc ttttccttct
    40261 tcttttttta ccgattcgtt ataaggaatc cgatgtcctg tcactagcaa acgatgcgtg
    40321 ttgatcatat acggcgtaca tgtaagcaag gtaactaagt cttctccttc tgaaatcgtt
    40381 agaacagacg tgtcatctgg cagtaccact tgtatatttt taacttgata agccaatttc
    40441 tcccccaaaa cagtcaaaat aaacgtgtct ccgttttcta aacgatctaa ttcggtaaat
    40501 aaccgtcgat taggtaaacc gctgtgagca gagatcactg aatgcgtcga atttccacca
    40561 agcgggaaag aggttccttg caagacgcct gctccatttt caaggatcct accattgagc
    40621 cgatcataaa gtgaaatatt taattggatc ttaggtatat tgatacttcc gatcagccat
    40681 tcctctaatt gactggatgt tgtactttca tttctgttct tttcattgaa aggatcagta
    40741 ccaggattga atccttggtc agttaatcgt tggtttttct cctccatttt ctttcgctgc
    40801 acttcaattt cagctgcggt ccgtttttgt acttgttcga tacgtttttg atcaataaaa
    40861 ctatttaaag aatccacata aaatggatat aaagcgatca gtaaccctac gacgatacat
    40921 attaataaaa acaaacgaaa caaaagtcga cgagactgtt tttgcttact ctgcttcaca
    40981 tgtgtcctcc cttgcaagtt tttttgttct ttcttttatt atatgggttc aggaaaaaaa
    41041 tagaaaatga aagccgatga ggttgtgaaa aagctgtcct gcatcaagta ataagtacag
    41101 cccaaaaaaa tagctactca tattttttgt tgggttgggc ttattaccgc agatgcaagc
    41161 ttttgaacac cgtttaagga agaggttgtg aaaactgcgt tatgctttga gtaataagag
    41221 cgctagtcag aaaatagctt ctcatatttt ctaggctagt gagcttatta ccgagaagca
    41281 gctgattgaa caccgtttac cgagaggtca tgaaaaagct actttgtttc gagtattaag
    41341 aaaaattctg tttcaaaaca aggagatgca acaaaactta tgtcgcatcc tctctcctta
    41401 gaactttcac ttatgttttt cttgtaaaat tcataaatat tttcatgaat tttctaattt
    41461 cttgcctacc tctcgcatag gatatatgat gaatttatct aaagtttgca aaggagttcg
    41521 acaattgaaa aaatatggcc catttttagc aatttttttc attgctttgg catttctcat
    41581 gcctcattta ttcacacata aactaatttt gggtgctgat gccccattcc attacaatcg
    41641 gttctatgat gccgcgcaac aaattaaata tggggatttc cattattttc cttcccttta
    41701 tggattccag caaagtggca ggatcgtgaa tgccgtttac ggcccatttt tcgcttattt
    41761 acaaggattg atcgtgttgc ttgcaggtag ctggtttcat tatcagattc tttcaaattt
    41821 cattctttat cttatttcag gattttctat gctgatttta ctgagatatg tcaatgttcg
    41881 aaattgggta agtgtaccag tagccgtttt attcatgagt acgtattcta ttcaatactg
    41941 gtctatcaac caaggattca ctagctgggg gacttgtttt tatcctttat gtatgatccc
    42001 tattgctgat ttcgtccttc ataaaaagtt ccctgttatc aaagtcgctg tctcagtggg
    42061 acttatgaca cagatccatt tactgtcttc gatcatgtta gctttgatgt atatcccttt
    42121 ttatctttat tactttttta accagactga aaaaggaaaa gcactgcttc aactgattaa
    42181 gtcggtcttg ctttattttg ttctgacagc aaatatttgg gcaggattag ctattgttta
    42241 cagtgggaat aagattcttc ctccctttgt taacagagaa atgagtcaaa aggcgataga
    42301 tctggatggc agttattggc tccattaccc taaggccttt cctatattga tattatcagg
    42361 ttttttgctt tttattttct ttcaacaaaa gaacacctta ttccaaaaaa tcgtgttgta
    42421 cacaagtctt gttttctttt ttctttctac tagcttgatt ccatggacga cattgatcga
    42481 aaaaaatgtg ccttttatta gtttgatcca gtttccattt cgatttttcg tgccatttac
    42541 gctattgctt ctactttacc ttgccctttc actgaaccag tggtctgaac aaaagtggtt
    42601 ccgtctttta agtatatttg gcattcttat tttttcagca cagacgatcc agaacttata
    42661 ccagcatctg gaaaagtggg aaaatgaaac atttgtcagt cgacatactt atctattcga
    42721 tacgccggaa gaagcaagga aaacattctt cagcaaagat atgtctacca gcttgttcgt
    42781 tttccaaaaa accacacctg actatctgcc aatctataag gagacaaaag acaataaata
    42841 cgaccggtat aacgaagaaa tactagaaaa cgaaacaact tttataaaaa cacatccagg
    42901 cggtcttttg acaatcaagt ggaaaaacac tggggacaaa actgttcata tccctgttat
    42961 cgtctacgat cggactgtgc tacagcaaaa tggaaaaaca ttgactgatt atgaagtaac
    43021 agatatcggg accccaatcg tgaagcagca aaaaggaatc aacgagctca cgcttcatta
    43081 tcaaacgcca atctatttct atttcattct ttctcttact ttgattggct ggtttacctt
    43141 gctatgttta tttatttatc atagatacaa gcttttacgt gcttaggcaa aagggataaa
    43201 tacatccgat caaaaaactc tccaaacggt ataatcgttt ggagagtttt attttaatca
    43261 gtttttttat gttatatgtt atatttttta ttcgttcaca tctaattcga tttccacccc
    43321 gtagtgatca ctgatgattg gactatcgat cccatcaaaa atccgttgat attttttaac
    43381 ctgcattcct tcagggacga aaataaaatc tatacgcaat ttttctgtat tttgttccca
    43441 accatctatt ttcttttcca cagtagctgc ttttcctttt tcattcgcga caacatacga
    43501 atcttgaaga agaaggcaac tttctcttac agtataataa ccttgagagt ctactggatt
    43561 attgaaatca cctaggaaaa aaagtggtac gcgagtttcc aaaaagtagt tttccaagtt
    43621 tttccactca aaataaaagc cacttttttt atctgtccac caagaaaagt ggcagctcga
    43681 gacaaaaatg tcctgatcat ctatctttgt tttcccaatc aagatcttgc gtgtctgata
    43741 atcttctttc ttttgaaatt gggagacggt aaggacctga ctttccaatg tttttttcgt
    43801 aagcagtgca tttccttctt catatttatc ataaccgatg tggctcattt cccaactcca
    43861 aaaatactct tttccgtgtt cctttaaaag ctgaactaaa cggtaggcaa aattatcttt
    43921 acggataggt acttgattct ttaccggaca gaacttcatt agcttcgcag gttctacttc
    43981 ttcagattcc agtaattggt tgatttcttg taaagcaatc acttcataat cttctaacag
    44041 gatcttatct gctaattctt gcaatttttt ctctggttct ggctcaagcc agctatgcgt
    44101 gttgagtgtc atgaatttca ttggttttct ccttgtcttc atttcgaaac aaaagggttg
    44161 tgacaaaatt tttgtcacaa cctctccagc aaatagtgtt ctcctgctaa ctcctcagta
    44221 ataagctcaa tggaccagga gctatttttt gtttggctgt ttttatcact tggtgcaggg
    44281 cagctttttc acgacctctc cactaaacag tattcaaact gctgcttctc ggtaataagc
    44341 ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatg acttgatgca
    44401 ggacagcttt ttcacaacct ctccttacat tttaatggag ccgatcactt taccagctgt
    44461 cactgtacct aattgcttga tcactacttc ttctacttta tcgtttgtaa atacgatcat
    44521 gatcgttgga tctttttctt cgttttggat cacatcaaga tccatctcag ctaatttcgt
    44581 tcctgttcct acttcttggc cttctttgac agcaatctca aaagctggct gattcatttg
    44641 gactgtatcg attcccatat ggatcaaaac ttcgatacca tctttcgttt ccagcccaat
    44701 tgcatgtttg ctagggaaaa tacttgtcac ttttccagcg atcggcgcaa caacttctct
    44761 tgatgttgga ataacagcaa agccgtcacc catcattttt tgtgagaata ccggatcttt
    44821 tacttcagat aaaggaatca cttctccaga agctgcggaa actagttctt gttctatacc
    44881 taagtattgc ttactacctg cttgtccatt tcctgtagaa accatttctg gctctggaat
    44941 ctctgctcct gattgcagca gatcttcgat atctgatttc aagacatctg cttttggtcc
    45001 ataaactgct tggactccat tgtctttgac gatcaatccc atcgcccctg ctcgtttcca
    45061 ggcttcttca gtccctactt tttctcgatc atttacactt acgcgtaacc gagtcataca
    45121 agcatcgaca tctttgatat tttcttttcc tcccagcaaa tgaatgatct ggaccacttg
    45181 ctgatctgct gttcctgttc cgctgttaga tacaacggtt tcttcactgt cattgtcgta
    45241 atttccgtta cgtcccggtg tagcaaagtt gaattttttg atcaagaaat ttgctaagaa
    45301 atacgtaaca acaccaaaag cgatcacaca caacacgaag ttgataagat ctccacctag
    45361 acccgctttg atagctaacg gtgtccgagt cagcaattcg atattcccaa atgaatggac
    45421 ccgcaatggc aagatatctg ccatcgcaaa tgcagcccct tggatcactg aataaatgac
    45481 atataatggc acagcagcaa acataaacat gaactccaat ggttctgtta cacctgttag
    45541 aaagacagcc aacgctgctg aaaaatacat tgatttatat cttgctttct tatcaggatc
    45601 gacattacga tacatcgcaa aagccattcc catcaagata cctgaagaac caatcatttg
    45661 tcctactttg aaacgagccg gcgtccagtt atgcaacaca aactcatatt gcgacatatc
    45721 ccctgcacct ttaagattca ctaaatcagt ggcccaagcc aaccataacg gatcttgccc
    45781 aaatacttgt gtccccgcct gtgcgcctga taaaatctca tacgatccgc caagctgtgt
    45841 atagttgata ggaatcgtca gcatatgatg caatccaaat ggcaatagta aacgttctaa
    45901 tgttccaaat aaaaacggtg ctagaatcgg tgcgctttct tgtgattctg caatccataa
    45961 accaaaatta ttgattccag cctgtacgtt tggccacacc acagcaagaa tcaatgccac
    46021 gattgttgac cataagataa caacaaacgg aacaaagcgc tttccattga aaaatgacaa
    46081 agcatccggc aattttctat aattatagta tttattataa gccattgccc cgacaaatcc
    46141 agcgatgatc cctacgaata ctcccatatt caaagctggt gcttccaata cacttgtaaa
    46201 aaatccttta accatgatct tcgtaccgaa cagagtgtgg gtaaatgcat cttcattcgc
    46261 aagcatgtca cttgtgacac caaagatagc cccagtgatc cgattgatta aaataaatga
    46321 gatcccagct gcaaatgctc cgcctgctct ttcttttgcc caacttcctc ctattgctaa
    46381 agcaaataaa atatgaagat taccaataat tgcccaacca atattttcaa ttactccgcc
    46441 cgttgtgaca agcggcgtcc aatcaggatt gatcaaggga attgatttcc ctatactgat
    46501 catcaaccca gctgccggca taactgctac cacaaccatt aaagcctttc caaatttttg
    46561 ccaaaattca aaactaaata tctttttcat tccgtttagc tcctttacgc aatcggtttc
    46621 gttaaacgta ttatagcgtg cgttttcttt tttgtaaatg ctttattttt tatttttctg
    46681 tctataccat tctataaaac ctataatacg tgtgttccct tctataattt tctcctaaaa
    46741 atttattttt ttaagctttt ttctatgaaa tattttcttt tgtctattga cttgtgcaat
    46801 cggttgcgtt tattatgaac gtataaaaca gctcattagg aggagaaaaa tgaaacagat
    46861 caaacgacta tttgctattg atccatggaa aatcagcacg acacaattag ataaaaaaaa
    46921 tcttcgactg caagaatctt taacaagtat tggaaacggt tatatgggga tgcgcgggaa
    46981 ctttgaagaa acttactcag gggaccacca tcaaggtact tatttggcgg gtgtctggta
    47041 tcccgataag acacgtgtcg gctggtggaa aaatggttat ccagaatact ttggaaaagt
    47101 gatcaacgcc ttgaacttta tcgcgataga tatttcgatc aacgatcagc tgatcgactt
    47161 ggcttctttg gaaccagaag acttttactg ggaattagat atgaaaaatg gtgtattatc
    47221 tcgtacattc acaatcacta cttctactaa taaagtacgc atttccttcg aacgttttct
    47281 gagcattgtc aaaaaagaat cagcttatat ctatttaact atagaaatgc ttgaaggtag
    47341 cggaacagtc aaactcgtat caaaattgga tggaaatgtt caaaacgaag acagcaacta
    47401 tgatgaacat ttttggaatg agattgatcg tggaactata gaagaaatcg gctatttgac
    47461 gactaaaact attccaaaca actttgggat tgaacaattt actgttactg cagcaatgcg
    47521 ccatttcgtt aatggaaaat cagtcatccc tgactatcac gaagatactt tagcattaca
    47581 tggttgcttg tctgcagaat tatctcaagg tgagacatgg tcagtggaaa aagaaattat
    47641 cgttcttact agtagagata ttccagaagc gcagcaagca gaaaaagctg tctcagaatt
    47701 aacgaaccag acaaacaatt atatccaagc aaaagaagag caaacagcgg cttgggcaga
    47761 acgatggaag cttgccgatg tggtcatcga aggagacgaa gaagcacaac aaggtattcg
    47821 attcaatttg ttccaactat tctctactta ctacggcgaa gatgaacgat tgaatatcgg
    47881 ccccaaagga ttcacaggag aaaaatacgg cggagccact tactgggata cagaagcgta
    47941 tgctgtccct ctttatcttg ctttagccaa acctgaagta actaagaatc tgctgaaata
    48001 tcggcataat caactgccgc aagcaatcca caacgctcag caacaaggat taaaaggcgc
    48061 gctttatcct atggtcacct tcactggtgt cgaatgccat aacgagtggg aaatcacatt
    48121 cgaggagatt caccgaaatg gcgcgattgc ctatgcaatc tacaattata cgaattacac
    48181 cggcgatacg gaatatttga aaaatgaagg actggaagtt ttagcagaaa tcgcacgctt
    48241 ctgggcagac cgtgtccatt tttcaaaacg agcaaataaa tacatgatcc acggtgtgac
    48301 cggcccaaac gaatatgaga acaatatcaa taacaattgg tatacaaata cgatcgcagc
    48361 ttgggtcttg cgttacacgc gagaaaatta tttaacttat caaaaagaag ctgctatcac
    48421 tatcagcgaa gaagaactag ccaaatggaa agatatcgca gagaacatgt acttcccagt
    48481 tgacgaagaa ttaggaatct ttgtccagca tgacacattc ttagataaag atctcatgcc
    48541 tgtatccgat attcccacaa gcgatcttcc tctgaatcag cattggtctt gggacaaaat
    48601 ccttagatct tgcttcatca agcaggcaga tgtactacag gggatttatt tcttcaatga
    48661 tacttttaca aaagaagaaa agcaaaaaaa ctttgatttc tatgaaccaa tgactgtcca
    48721 tgagtcttcc ctttcgccaa gtatccattc gattttagca gcagaattag gaatggaaag
    48781 caaagctgta gaaatgtacc agcgaacagc tcgcttggat ttggataatt acaataacga
    48841 cacagaagac ggcttgcaca tcacttccat gactggtagc tggttgtcga ttgtccaagg
    48901 atttgctcaa atgaaaactg cccatgatca gttgagattc gcaccatttc tgccaaaaac
    48961 ttggaaagct tattctttcc atatcaatta tcgcggacgt ttattgttca tctcagtcac
    49021 agatgaacaa gttactttcg agttattaga aggttctcct ctagaaattt acgtctatga
    49081 acagtcttat tctttagcgg atcgtctaga aataacgcta agaaaggagg aagcaaatgt
    49141 ttaaaggggt attgtttgat ttagacggtg tcattaccga tactgctgaa ttccattatc
    49201 gtgcttggcg aaagcttggt caggaaatcg gcattacgat cgaccacaca ttcaacgaac
    49261 agctaaaagg cgtcagccga gaagactcac tctctttgct tttagcttat ggcggaaaag
    49321 aacactcttt ttcaaaggaa gaatttgcag aattagcgaa aagaaaaaac gattattatt
    49381 tagagatgat ccagacgatc gaaccaaaag atgtcttccc cggggtcgtg cctttactgg
    49441 attctttaaa agaagaaaaa atcaagattg ctcttgcttc tgcaagtaaa aacgggccat
    49501 tcctacttga aaaaatgggt cttacacctt actttgatgc tattgccgac ccggcagaag
    49561 caacaaacgg aaaacctgcg ccagatatct tcctccttgc agcaaaagct gtcggactaa
    49621 aagcagaaga atgtatcggg atcgaagacg cgcaagccgg tatccaagcc atcctctcaa
    49681 gtggcgctca accaattggc gtgggacgtg ctgaagacct tggagaggag atcccgcttg
    49741 tccctgatac gacttttctt acgatcgatt acttgaaaga aaagtggcat gaccatggat
    49801 aatccagaga tctccgtcgt accagttcca gagactgaaa taaagaaaat cacattgaag
    49861 aacgactact tagaagtcgt tcttctcaat tacggtgccc gcctgcacca aatcttcgca
    49921 ccagacaaag aaggaaaatc agaaaatgtt ttattgtctt acgatcgttt cgaagatgtt
    49981 ctaacagaca aaagcttttt tggagcaact gtcggacctg tagccggaag gatccgggat
    50041 gcttcttggg gtgagcatag attggagaaa aactgcggat cacaccatat ccacggtggc
    50101 acgaaaggct ggtctttcca attttgggaa gtagaagttt tcaaaactcc atcatcaata
    50161 ggtgtggtct tttacttaaa agatgaattt tctacctatc caggaccaat cactgctacg
    50221 atcacttacc gtttaacaaa aaacgaatta gagatgacaa cctctgctag tagccttgtt
    50281 gaaaccatct gcaatccgac caaccatgcc tacttcaacc tttcaggaaa tggaacaaga
    50341 gacatctatg aacatcagtt aacggtcttt ttagatggtg tgcttgaatt agatcaagaa
    50401 aaactgccta ctggaaattg gacaaaaaaa gaaaaactgc caatcgactt tagaaaatcc
    50461 ccaacactgc aagagatcct tgcatgttat ccagatggac tagatgatgt atttcttttg
    50521 catcatccta ggctttccaa aacttcctta caattattcg agaagcattc tggtcgccaa
    50581 atgactatag caacttctaa taaaagtatg gtattatttt ctacgactgg attcgaagct
    50641 gatttctctg tcaatggaaa acagatgcat tcaaactacg gattagcaat cgaaccgcaa
    50701 gaaatccctg atatcgttca tttccccaaa tttggatcga tcaacctcca tcctggtcaa
    50761 gaacggatca gtcagacgat ttatcggttc tctgcacaat aacaagagaa aggtgttttt
    50821 atcatggcta ttaccgtaaa agatgtggcg aaaaaagccg gtgttgccac ctcgaccgtc
    50881 tctcgtgtca tcaacgatca tcctagtatt tccgagacaa caaagaagaa agtccgaaaa
    50941 attatggatg aactaggtta tgttcctaac atgactgccc gcaatctagg aaaacgcatt
    51001 tccagtgcca tcggcgtaat cttaccccct cttgattcaa aagagcggat cggtaaccca
    51061 ttttatcttg agatcatgga agcaatcaac gaagaagcgc ggaattatga tatgactaca
    51121 gcaatcgcta ctgctaaaaa ttttgaattg ctgttagaga acgtccagcg tatgcattta
    51181 caaaaacaag tagatggttt tattctagtg tattcagata gtcaagatac tgtcattgat
    51241 tatttatacc aaaaccacat ccctttcact ttgatcggac agccttatca tcatgagaac
    51301 gaaatcgttt atgtagataa tgataaccag cttctaggta aacaagcaac agaatttttg
    51361 atccagaatg gacataagaa tatcctgttt gtgacaaaca cgactcatga aagtttgtat
    51421 ttcgaacgat atttcggtta tcaaaaagca atgatgatgg ctggtcttcc tgcgttatcc
    51481 agtgtagtac tagagcatcc ggaggattat ctgtcctttg agaaaacgtt gaaagaaaac
    51541 gaagccacag cattggtggt cattgacgat atcttcgccc ttcgcacgat gcagttggcg
    51601 caaatgtatg gctatgtcat tccagagaca ctctccgtca ttagcttcaa caactcgatc
    51661 ttctcaacat taacacaccc ttaccttaca agtatcgata tcgatatctc cgaattagga
    51721 cgtgtcgcta tgcaaaagct gcgcgaattg atccatgaag aattgagcag tggcgtacgt
    51781 ttggtcatcc ctcatcgctt gatcaaaaga gagacagtcg ttcctttaga aaacccatac
    51841 taaatagctg actcactaaa tacgctgacc atttatccta tggtcagctt tttatgtaaa
    51901 agaagatact acttctaaca cattgtttat ctg
//
LOCUS       DACGLH010000013        47259 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.014, whole genome shotgun sequence.
ACCESSION   DACGLH010000013 DACGLH010000000
VERSION     DACGLH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.014"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..680
                     /locus_tag="IXT21_000828"
     CDS             <1..680
                     /locus_tag="IXT21_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4868256.1"
                     /translation="SFQVKERQHQESLQLYQINEEYYHHLEEFKHDYKSLLFSLKITL
                     QENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAESEKIPIQV
                     VIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRFSIANKAN
                     PTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVEMYLAS
                     RKSKSAY"
     gene            1002..1229
                     /locus_tag="IXT21_000829"
     CDS             1002..1229
                     /locus_tag="IXT21_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ4868257.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLMHTHGGYLNY"
     gene            1352..1678
                     /locus_tag="IXT21_000830"
     CDS             1352..1678
                     /locus_tag="IXT21_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ4868258.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1746..2459
                     /locus_tag="IXT21_000831"
     CDS             1746..2459
                     /locus_tag="IXT21_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ4868259.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2769..3619
                     /locus_tag="IXT21_000832"
                     /pseudo
     CDS             2769..3619
                     /locus_tag="IXT21_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            3740..5605
                     /locus_tag="IXT21_000833"
     CDS             3740..5605
                     /locus_tag="IXT21_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4868260.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5653..7062
                     /locus_tag="IXT21_000834"
     CDS             5653..7062
                     /locus_tag="IXT21_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ4868261.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            7170..7871
                     /locus_tag="IXT21_000835"
     CDS             7170..7871
                     /locus_tag="IXT21_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4868262.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            8030..8509
                     /locus_tag="IXT21_000836"
                     /pseudo
     CDS             8030..8509
                     /locus_tag="IXT21_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            9356..9931
                     /locus_tag="IXT21_000837"
     CDS             9356..9931
                     /locus_tag="IXT21_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ4868263.1"
                     /translation="MKFTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            9931..10371
                     /locus_tag="IXT21_000838"
     CDS             9931..10371
                     /locus_tag="IXT21_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ4868264.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            10387..11088
                     /locus_tag="IXT21_000839"
     CDS             10387..11088
                     /locus_tag="IXT21_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4868265.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(11576..12001)
                     /locus_tag="IXT21_000840"
     CDS             complement(11576..12001)
                     /locus_tag="IXT21_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868266.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            12366..13565
                     /locus_tag="IXT21_000841"
     CDS             12366..13565
                     /locus_tag="IXT21_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4868267.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            13587..13799
                     /locus_tag="IXT21_000842"
     CDS             13587..13799
                     /locus_tag="IXT21_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ4868268.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            13874..14350
                     /locus_tag="IXT21_000843"
     CDS             13874..14350
                     /locus_tag="IXT21_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ4868269.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            14347..16155
                     /locus_tag="IXT21_000844"
     CDS             14347..16155
                     /locus_tag="IXT21_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ4868270.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            16232..16447
                     /locus_tag="IXT21_000845"
     CDS             16232..16447
                     /locus_tag="IXT21_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAQ4868271.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            16539..17402
                     /locus_tag="IXT21_000846"
     CDS             16539..17402
                     /locus_tag="IXT21_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868272.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            17433..17975
                     /locus_tag="IXT21_000847"
     CDS             17433..17975
                     /locus_tag="IXT21_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAQ4868273.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            17989..18411
                     /locus_tag="IXT21_000848"
     CDS             17989..18411
                     /locus_tag="IXT21_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ4868274.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            18341..20740
                     /locus_tag="IXT21_000849"
     CDS             18341..20740
                     /locus_tag="IXT21_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ4868275.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            20772..22763
                     /locus_tag="IXT21_000850"
     CDS             20772..22763
                     /locus_tag="IXT21_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ4868276.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            22786..23037
                     /locus_tag="IXT21_000851"
     CDS             22786..23037
                     /locus_tag="IXT21_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAQ4868277.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            23027..24256
                     /locus_tag="IXT21_000852"
     CDS             23027..24256
                     /locus_tag="IXT21_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAQ4868278.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            24253..26334
                     /gene="topB"
                     /locus_tag="IXT21_000853"
     CDS             24253..26334
                     /gene="topB"
                     /locus_tag="IXT21_000853"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4868279.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            26483..30403
                     /locus_tag="IXT21_000854"
     CDS             26483..30403
                     /locus_tag="IXT21_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAQ4868280.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITKIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            30404..31348
                     /locus_tag="IXT21_000855"
     CDS             30404..31348
                     /locus_tag="IXT21_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868281.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            31352..31555
                     /locus_tag="IXT21_000856"
     CDS             31352..31555
                     /locus_tag="IXT21_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868282.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(31855..32091)
                     /locus_tag="IXT21_000857"
     CDS             complement(31855..32091)
                     /locus_tag="IXT21_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4868283.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(32274..32645)
                     /locus_tag="IXT21_000858"
     CDS             complement(32274..32645)
                     /locus_tag="IXT21_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868284.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(32642..33970)
                     /locus_tag="IXT21_000859"
     CDS             complement(32642..33970)
                     /locus_tag="IXT21_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ4868285.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(33931..34260)
                     /locus_tag="IXT21_000860"
     CDS             complement(33931..34260)
                     /locus_tag="IXT21_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ4868286.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(34518..34889)
                     /locus_tag="IXT21_000861"
     CDS             complement(34518..34889)
                     /locus_tag="IXT21_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4868287.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            35034..35219
                     /locus_tag="IXT21_000862"
     CDS             35034..35219
                     /locus_tag="IXT21_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4868288.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            36219..37025
                     /locus_tag="IXT21_000863"
     CDS             36219..37025
                     /locus_tag="IXT21_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAQ4868289.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            37043..37870
                     /gene="vanW"
                     /locus_tag="IXT21_000864"
     CDS             37043..37870
                     /gene="vanW"
                     /locus_tag="IXT21_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAQ4868290.1"
                     /translation="MDRKRLTQRFPFPLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            37867..38838
                     /locus_tag="IXT21_000865"
     CDS             37867..38838
                     /locus_tag="IXT21_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ4868291.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            38831..39859
                     /gene="vanB"
                     /locus_tag="IXT21_000866"
     CDS             38831..39859
                     /gene="vanB"
                     /locus_tag="IXT21_000866"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ4868292.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAVCMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            39865..40473
                     /locus_tag="IXT21_000867"
     CDS             39865..40473
                     /locus_tag="IXT21_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ4868293.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            41061..41492
                     /locus_tag="IXT21_000868"
     CDS             41061..41492
                     /locus_tag="IXT21_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4868294.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            41499..41732
                     /locus_tag="IXT21_000869"
     CDS             41499..41732
                     /locus_tag="IXT21_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4868295.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(41654..41896)
                     /locus_tag="IXT21_000870"
     CDS             complement(41654..41896)
                     /locus_tag="IXT21_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868296.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            42153..42356
                     /locus_tag="IXT21_000871"
     CDS             42153..42356
                     /locus_tag="IXT21_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ4868297.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            42440..43633
                     /locus_tag="IXT21_000872"
     CDS             42440..43633
                     /locus_tag="IXT21_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4868298.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            44073..45044
                     /locus_tag="IXT21_000873"
     CDS             44073..45044
                     /locus_tag="IXT21_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4868299.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            45076..46473
                     /locus_tag="IXT21_000874"
     CDS             45076..46473
                     /locus_tag="IXT21_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4868300.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            46632..47255
                     /locus_tag="IXT21_000875"
     CDS             46632..47255
                     /locus_tag="IXT21_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868301.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        13348 a        10733 c        11986 g        11192 t
ORIGIN      
        1 tcagtttcca agtaaaagaa agacaacatc aggaatcgct tcaactgtat caaataaatg
       61 aagaatatta ccatcatcta gaggaattca aacatgatta taaaagtcta ctgttttcat
      121 taaaaattac tcttcaggaa aataaaaaag aaacgttgga atttttagaa atattagaag
      181 atcattctgc agctattttt gaaaaaccgc aaatacaaca gctaacaaat attgtatccc
      241 cagctgtcag gggtgtcttt ttaaaatttc tagaaaaagc agaatcggag aaaatcccga
      301 tccaagtagt tattcctgtc gaagtgaaaa caatcccgat tgatctggtt gtttttattc
      361 gctgtttatc tatttttatt tctaacgcat tcgatcatcg tgtagccgat cagagtccga
      421 ttacgatcaa tctgctggaa gaaaaagagg gatcacgttt tagtatagcg aacaaggcaa
      481 atccaacgaa tttgacgcta tcggagatgg tcaaaagagg aaaaacctct aaaaaaaatg
      541 gcgggttagg tctatacagt gcgaaaaaaa tgttggatgt ttacgaaaat gcagaactaa
      601 aaatcgaatt ttcaaaacag aatcactatt tttttgtaga aatgtatctg gcatctagaa
      661 agagcaaatc agcctactga cttactgtca aaggataagt ttgaggaaag tctacacgga
      721 gatagagaca acaacacagc atattcaaaa gaaacagaag cgtgatgacc gttgtcaccc
      781 ttctgtttct tttgctatgt agttaaacaa agtagttgac acttccgcta cttagtatta
      841 gtacgaaagt atacttcatg atactaagta gataagaagt gaaaagcgga acccacgatc
      901 gtcaaaatgc taactgcttt gcttacacca gataaaggga agagcaatat cgcttatttt
      961 aggcgtggag aagcaagcaa atccctacga tcacaaaaaa aatgctgacc cagcagttaa
     1021 gagagttaga acaagatgaa agtatccatc gaaaagccta taaccaagtc ccgcctaaag
     1081 tagagtactc gctcaccgaa gaaggaaaat cattaagaga gattctagta gcgttgtctg
     1141 tttgggaaga agaacgtatc gaaaaacaaa aagcctcaag ggaagaagac acgctaatgc
     1201 atacacatgg tggctaccta aattattgat tttctatgac atttatttta aataaaagaa
     1261 aacaggagtt tttttaaaag catcatctat tttttatgta aaaaaatggt atgattaatc
     1321 aagtaagaag tgattggaag aaggcaagca aatgaatagt atggaaaaag tcaaccagtt
     1381 tagagatgaa cgtaattggc gacaatttca taacgaaaaa gatttggcta tctctatttc
     1441 tttggaagca tctgaattgt tagaactttt tcaatggaaa acgccggaag aagtaaaaga
     1501 aacacaatta gaacgaatca aagaagaact ggcagatgta ttgatctatt catacatgat
     1561 ggcagacaac cttcacctgg atctagatga gatcattgaa gaaaaattga tcaaaaataa
     1621 tttgaaatat cctgtagaaa aaagcgcagg tgtacgaaaa aaatacacag agttgtagaa
     1681 atgtgtcatt cggctgttgg aaagagcaaa agaaaaacag taaaatgagt ctggagggag
     1741 agaaaatgaa aaaaaaagga aaagcaaaaa gatggctagt caatatcctc ttgttcctct
     1801 tgttattagt aggactagct ttgattttca atgaacagat caaggattat tttgtcagag
     1861 agacaggaga taaatatgct atagcgaatg taacaaaaga agatctgaaa aagaataacg
     1921 ataaagatgt cagttttgac tttgatgcgg tagaaccaat gacgacagaa ggggtcatga
     1981 ggtcgcaaat gagaggaaca gatctaccag tgattgctag tatcgcagtc ccttcagttt
     2041 caatcaattt accggttttc aaaggcttgg ataatacgtc actactttac ggagcaggaa
     2101 cattatctcc agatcaagaa atgggaaaag gaaattatgc cttagcaagc catcgtgcaa
     2161 cgaaccctga attgctgttt acaccgctag aaaatttaga gatgggtgcc aagatctatt
     2221 tgacagattt agaaaatgtg tatacataca aaacattttt taaagaaaaa gttgctccta
     2281 cagataccca attactgaat gaagtcgagg gaaaagaaat tgtcacacta attacttgtg
     2341 gcgacatgga cgccgtcacg cgactagttg tacaaggaga actggaaagt gttacatcga
     2401 taaaagatgc gacagatgat atgagatctg ctttcaatct agagacaaaa acattttagg
     2461 atactgggac ataagtcgtt acagattaaa aacagccaaa ggatatgaat cgataaggaa
     2521 aaaccaattt gctgaaaaaa agagaaacgc aaagaggtgc atctctgatt tggttgtttt
     2581 ctagatagac ttgtgttcca gtttttttat tttttatatg aaaaggcttg cataaataaa
     2641 aattcgatgg tatgatgatt ttgaataaga aatggattgt tactgtacgg caggcaaaac
     2701 cagaattcag ctaagaaaca gtaggcgaat tctaggtttt tttatttttc ttgagagggc
     2761 gaagcaaaat gaaaattgat aaaattttga ataacaatgt cgtcatttcg aaaaatggat
     2821 tcggggaaga agtcgtttgt atgggaaaag gactagcttt tcaaaaaaag attggggacg
     2881 agatttcgcc agaagcggtc caaaaagaat ttgttttaag agattctttt gctaaaaacc
     2941 aattccagca gctgatggct gatattccgg cagaggagat cgaatgggtc aaacaagtag
     3001 tcgaactggc tgaagataaa ctaaaagttg aattttcgcc taatatttat ctgacattga
     3061 ccgatcatct tcattatgcg atcacacgag caaaagaaaa tatccaactg cctaatccat
     3121 tattatttga aacaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt
     3181 gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt
     3241 tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac
     3301 aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga
     3361 atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa
     3421 aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc
     3481 aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga
     3541 aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa
     3601 aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa
     3661 taggttcacg aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa
     3721 aagaaagaag gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt
     3781 aggaggacaa aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt
     3841 aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt
     3901 cgtacaaagt ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca
     3961 atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa
     4021 tattttgaat cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat
     4081 ggcagccgcc ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac
     4141 agctgatagc ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt
     4201 acctgtcatg cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt
     4261 agcaattgct atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc
     4321 tatcagtttc tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat
     4381 accaatcatt ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat
     4441 tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt
     4501 aacatttctt gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt
     4561 caactcgatt tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca
     4621 agtattcgtg atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga
     4681 acaatatggg tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg
     4741 ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc
     4801 ttctacagtg acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc
     4861 tttgaagaaa ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg
     4921 attttccgga gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt
     4981 cattaatacg gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat
     5041 tgcttttgtt cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga
     5101 caatcaatta gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac
     5161 cttaaaaagt cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt
     5221 ttcttctggc gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc
     5281 acctgcagac ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac
     5341 gactgacgga gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa
     5401 tggctttgag atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg
     5461 ttttgacatc gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac
     5521 gaatactgat gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga
     5581 agatgttctg gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa
     5641 aatttttatg gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac
     5701 ggaaaaggac tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc
     5761 ggcagtgaag aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga
     5821 ggaatcgatc attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc
     5881 aaatgttatc gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact
     5941 cctaacgaag cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat
     6001 attgaacctg tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac
     6061 ggtggctgga aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta
     6121 gagagataca gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt
     6181 cactttccag ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat
     6241 attttccaag catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac
     6301 gaattgcgtt cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc
     6361 gatgccaatc cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc
     6421 tgcacggatg tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa
     6481 tatggagtcg atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa
     6541 tatccagtgg actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat
     6601 ccacaagcag caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag
     6661 gcaagtgaat ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta
     6721 tacaatcgtt accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat
     6781 atggaagagg atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc
     6841 gaagcgatgg ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga
     6901 tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat
     6961 gtggatctag acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat
     7021 tggtataaag aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa
     7081 aaagaacaca cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt
     7141 ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag
     7201 tcgcagataa aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc
     7261 cgacagaaag tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa
     7321 tcgaactgct ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc
     7381 taagcaatcg tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga
     7441 actcccgata tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg
     7501 aagatccttt gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt
     7561 tgaacaatca gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca
     7621 gcagagaaga atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta
     7681 ttgactcatt ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc
     7741 tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg
     7801 aaatgattga atattcggag gcggtctacc atacggagct gcatccttac catattgatt
     7861 atgaaacata aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     7921 agccaaacaa aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca
     7981 cggctgtttc tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg
     8041 aatctatgaa ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac
     8101 gatcgcttat ttcttaggga aaatcattcg taaaaatata ttcaatcgtc aagaaatcga
     8161 tacattgaag accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat
     8221 cgtattgaac gacttggcac gcaatgtcat agcaacggga gtcggtgggg cagtaggtgg
     8281 cggattaagc atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt
     8341 cgctacgatg accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac
     8401 aggaataacc attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga
     8461 agaaaaagag gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa
     8521 tggattacaa gaaaaaagta caatagatgt accttgaggc aggaatggta tgaaagaatc
     8581 agagttagaa agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag
     8641 catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaaa
     8701 ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa
     8761 aaaggaaaga aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt
     8821 acacgttcga gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca
     8881 ctgaattttc ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc
     8941 gttaacaaaa agatttttag gataagctga tgaacaaaaa tgatcaaatt ttgatcgttt
     9001 agttcgtcgg cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac
     9061 aaaatgattt tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta
     9121 ttggcatata ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa
     9181 acacgttatt tatttttgtt ttatctataa aatagctgaa aaattatttt ttctaaatac
     9241 tatatatagt ggtgtctatc ctatatacca ctaaaaaact acaaaatcta gtcttttatt
     9301 ttaaaaaaac acgtgtagaa tgaagataga cttttgagtt agggagtgtt tttttatgaa
     9361 atttactgtt ttttgcggtt cccgttttgg taataaagaa agttacaaat ttattgcgca
     9421 aacgttagga aaatacatgg cacaagaaga aatcgaactg gtttacggtg gttcatactc
     9481 cgggattatg ggagtgatta gtaaaacagt actggaaaat aatggaaaag tcacaggtat
     9541 ctatccgaat gggctttttg aaactgaatt gcctggaaaa gatgttacta catttattcc
     9601 tacagaaacg atcgatgaaa gaaaggtatt attatttgaa aagggagatg ctgtcctcgt
     9661 tttccctgga ggtctaggta cattggagga attctcacaa cttctttctt ggatagcaat
     9721 cggtctgacg cctgacaagc caatcggaat attgaatatt ggcggttatt acagcggctt
     9781 gcaacaatta ttagaaactt ttgcaaaaga ggattttatg gataaaaaat ggttggatca
     9841 agtaatcttt tccaacaatc cattggaact ggtcaatgaa ttacgagcag tatctattaa
     9901 aaatcagtta ttattgaagg aggcaaaata atgagtcaaa tatatttagc aggaccattt
     9961 ttttcagagg agcaaatcga tcgagtaagt cgtatcgaaa aagcgttgga ggaaaacaaa
    10021 acagtcacaa gtttctactc tccacgtcat caccaagaaa gcaattatga actattcagc
    10081 gctggatggg cgcaggaagt atacgaaaaa gatatggaag aactgacaaa tgcagaattt
    10141 gttgttgcta tccttgactt tgagcatcaa acaatcgatc caggaacggc ttacgaatta
    10201 ggtgtggcaa ctatgttgaa aaaaccgatg atcattgtac aagaagagac ggtacctacg
    10261 aacttgatga ttacacaaag tctccatacg tatttaaaat cagaccaagc tgtacgtgaa
    10321 tatgattttg aaacattgcc tgttgaaact tacgtaggcg agtacttata gaagaggaag
    10381 ggttgtatgt cagtgatttt gatttcagga acgattggtg caggtaagag cagtttgacg
    10441 gatatgcttg caaaagagat tgattctaaa ccattttatg aaaatgtcga agataacgaa
    10501 gtattgccgc tcttttattc aaatcctgag cagtacgctt ttttattgca aatcttcttt
    10561 ttgaacaagc gatttttagc aatgaaaaat gctttagtga acgatgacaa tgtgctagac
    10621 cgatccatct atgaagatag cctgttgttc catctaaacg ctgatttagg tcgggtaacg
    10681 gatatcgaag tacagcagta cgatagctta ttagatacga tgctaaacga gttagatgat
    10741 gttgctccaa aaaaacggcc tgatttgatg gtccatatca aagtttcttt ggacacgatg
    10801 ctagaacgta tcaaaaaacg tgggcgtgac tatgaacagc tagaaagcga tgagacgtta
    10861 tatacctatt atgaaacgtt gaacacccgc tataatcaat ggtatgaaga ttttgatgta
    10921 tgtcctaaaa tacaagtgga tggggataaa ttcgattttg tagaaaatcc agaagatgca
    10981 aaaaaggtga tccatcagat ccaagaaaaa ctagctgaat tggaaggaaa gcctgctgga
    11041 aaatatttta cggataacca gcatgcaaaa ggtcaagaat taggctaaaa ataaaaaaac
    11101 aaacgacggg aaagaaaaat tactacgtga tgtagaataa gcctttctat cgtttggtta
    11161 ttttatattc aaatatttga aataaataaa tctttactct ttgatttgta aactgaaatg
    11221 atccactgtt tgttttactg atgaagcttg atcaaaggaa gaagctgagt ttgaagttca
    11281 taaaaatcag ccatagtgag ttttgcttgg atttgtttat cttcttctac tttttacata
    11341 attttgaaca ggataagatt caaatgtttt aaatgaatct ttgactagtt tgtgacagtt
    11401 gtcgcatatg ctcaagcttc cttggtcatt aaattctgta tggatatttt tgttgttatt
    11461 acatgtacaa taatcaggac tatcgatcat ccatttcgtc tcctttacaa tcatcttgct
    11521 tctttactat agcagaaaag aaggggagaa acaaaagaag atccaacaga cggcactata
    11581 gactgtagat gttgccatcc tcatccttca aacggtaatt accgattttg gtaaatgtga
    11641 tggttgcttc ttttttattt tcttttcctg aaataatata ttcgtcatct ttggtagtaa
    11701 tcttgatgtc tgtcagcact gttttttctt cagatgattc agaagaatca gtttcttgtg
    11761 tctgatacgt gcgtgtccat tccatttttt tatctgtaaa tgtcaactgt cccgcggata
    11821 aatccatatc tttacgtact tttagcgtat atacactgtt agtgatatct gctttttccg
    11881 cttttaagac agaagaatcg gttgtttcat ccgaggattg gtcagagccg ccaaacaaat
    11941 tacagccagt caatgtcaaa agtcccgcag acaaaataaa tagtacgatt aatttcttca
    12001 aaatacttcc cccatttgat aggataaaga aattatacca ttggaaagta aaacttcaaa
    12061 cggatggaag agaaaaacgc acaaaacacc cacaaataga gtaaatttta tttgaaaatt
    12121 ttaggttatc gctgaaagcc ccggaaatac gggcatttcg caggatatga gaactcaatt
    12181 tactaccaat ttactacttt cggattgagc cgcatatatg ctgaaccata ctgctaatac
    12241 ccgccacccc aaatacggca catcataaat tgaatacgac accgtaaacg cggcgttcct
    12301 ctttcccgtc atgggagaac aggacgccgc ttttttatgc ccaaatagag aaaggaggcg
    12361 acgccatgct atttacttcc ggccctgacc gggcgtttga acggctgatg caggaaaagc
    12421 ccggtttcga tcactacgac ggcggcgggg cggacgcgcc ggaggattgc ggcacctgcc
    12481 gattttaccg cccggattgg aaatatcaat tctgccagtt tgcagagtgt ccctatcagc
    12541 cgggcaagct gaccgactat gcagcggtca aattcacagt gaaaggagcg aacaacgcta
    12601 tggcagtttt ccgtgtggaa aagaacaaag gctatacggt gatgtcaaat caccacctgc
    12661 ggaataagaa cctgacctta aaggccaagg gcctgctgtc gcagatgttg tccttgccgg
    12721 aggattggga ctatacccta aaaggactgt cctatatcaa ccgggaaagc atcgacgcga
    12781 tcaggacagc ggtatgggag cttgaaaaag ctggatatat ccggcgtgag cagggccgtg
    12841 acgcaaaagg caaaatggcc gatatggtct ataccatcta cgaacagccg gtattggaaa
    12901 acccggtatt ggaaaatcca acatcggata acccggtgtt ggaaaatccg acatcggata
    12961 atccggcgtc ggaaaatcca acgcaattaa ataaagaagt acaaagaact gacttatcaa
    13021 aaaaagaaaa aataattaca gatggacaaa gtacccattc cattcctatc ccatccccga
    13081 cccctgcccc tatggagggc gaggcagcgg ctacgccgcc ggaacggaaa aggacaggaa
    13141 aggatgaggc tgtacggatt tatcgggaaa tcattttgga gaacatcgag tatgcctatc
    13201 tcattcagga caggagcatt gaccgtgaac agcttgacga aatcgtagac ctgatgctgg
    13261 aaaccgtctg caccgcccga aagacaatcc gtgtcgccgg ggacgactac cctgccgagc
    13321 tggtgaaatc caagtacatg aagctgaccg gcgaacatat ccgctttgtc cttgactgtc
    13381 tgcgggagaa cacaacgaaa gtccgcaata tcaaacaata cctgcgggct atgctgttca
    13441 acgcccccag caccatttcc aactactaca cggcccttgt cgcgcacgac atggcacagc
    13501 ccgattgggg aaagcctaaa tccggcctgc cggactattc ctgcggcgag ggcgaaagcc
    13561 tgtaacgaga aaaggaggac gaccctatga agcagggcgc attgattttt gacgagcgaa
    13621 cagaccgtta cgacatccgc tttgatctgg ccgactatta cggcggcctg cattgcgggg
    13681 agtgtttcga cgtgatgatc ggcggcaaat ggaggcccac ccgcattgaa atggccgaaa
    13741 actggtatct tgtcggcatc cgggccgacg acctttccgg cctgcgggtg cggatttgac
    13801 ctatatctac ctgcggcgtc tggtggttcc gccttagacg ccgcttttga ctttctgaca
    13861 aggagggatg cgattgcagg aagaagttac tcaaaagacc attgccctgt ccatgaaaac
    13921 gggcaagctc acggctcaag tgctgcaagc ggcgctgaaa aagtacctgc aacaccgggc
    13981 caagggcaaa aacacgctgc accacggcca gcagagccta aagcagctta aaaagcatgg
    14041 ggctgatctc tccaacattg agatcaccga ggccaacatt ggagcgttca agccatgcgc
    14101 caagaaatac ggcgtggatt ttaccttacg aaaagataag accacccagc cgccccacta
    14161 cgttgtgatt ttcaaggcca aggatgcaga taaccttgat ctggcgtttc gggagtttac
    14221 cgccaagacg ctttccaagg agcagcggcc ctctatccgc aaggttttgt ccgctgtgaa
    14281 gcaaaaggcg gcggcccaga ccaagcagcg ggccaaggag aaaatcaagg aaagggggct
    14341 ggaactgtga agcctgacgt aaagaaattg ctgatcctga acctcccgta tctgattttt
    14401 gtgtacctgt ttgcgaaatg cggggaggcg taccggctgg ccgctggtgc tgacctgtcg
    14461 gggaagctcc tgcattttgc ggatggtttt gcggcggctt ttgccaaccc gctgccaagc
    14521 ctgcacctgt ttgacctgtg tatcggtatc gccggtgccg tgatcgtgcg gctggtggtg
    14581 tactgcaaga gcaagaacgt gaagaaatac cgcaaaggca tggagtatgg cagcgcccgc
    14641 tgggggacgg ccaaagacat agcgccttac atcgacccca agtttgaaaa taatatcctg
    14701 ctcacccaaa cggaacggct taccatgacc ggcaggccca aagaccccaa gacagcgcgg
    14761 aataaaaacg tgctggtgat cggtggcagc ggttcaggga aaacgagatt tttcgtgaaa
    14821 cccaacctga tgcaatgctt tccgaccacg gactatccga cttcgtttgt ggtgacagat
    14881 ccgaaaggca ctttggttct ggaaaccggc aggatgttcc agcaggcggg ctaccgcatt
    14941 aaaatcctga ataccattaa cttttccaag tccatgaagt acaacccctt tgtctacatc
    15001 cattcggaaa aggacgtgct aaagctggta aataccctga tttccaacac caagggcgag
    15061 ggggaaaagt cagcggagga tttttgggtg aagtcggaac ggttgtttta ttcggccctg
    15121 atcggctata tctggtacga ggccccagcg gaggaaatga acttctcaac gctgctggaa
    15181 atgataaacg ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc cgtcgatctc
    15241 atgtttgagc gtttggagca gaaagacccc gaccattttg cggtgcgcca gtacaagaaa
    15301 ttcctgctgt ccgcaggcaa gacccgttct tccatcctga tttcatgcgg tgccagactt
    15361 gcgccctttg acatccggga ggtacgcgag ctgatggagg acgacgaaat ggagctggac
    15421 accatcgggg acgaaaagac ggtgctgtat ctcattatga gcgatacgga cacgaccttt
    15481 aatttcattc tggctatgct ccaaagccag ctaatcaacc tgctttgtga ccgggctgat
    15541 gatgtgtacg gcgggcggct gcctgtccat gtacgcctga ttttggatga atttgccaac
    15601 attggccaaa tacccaattt cgataaactc attgccacca ttcgaagccg tgaaatatcg
    15661 gcctctatta ttttgcagag ccagtcgcag ctaaaagcga tttacaagga cgccgccgag
    15721 atcatttcgg ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa aaatgccaag
    15781 gagatttccg atgcgctggg gaaagaaacg attgacagtt tcaacaccag cgagaaccgg
    15841 ggaagccaaa cgtcccacgg attgaactat caaaaattag gaaaggagtt gatgtcccaa
    15901 gacgaaattg ccgtgatgga cggcggcaag tgtatcttgc agctacgggg tgtgcgcccg
    15961 tttttatcgg agaaattcga tattaccaag catccccggt acaaatacct tgccgacgcc
    16021 gacaagaaga atacctttga cgtggacaga tttttgacta tcacgcgccg gaaacggcag
    16081 caagtggtaa cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc ggacgaggac
    16141 gccgccgctg aataaacagc ggcttttttc attccaacaa caaaatcaaa aaatcattat
    16201 gagccggaaa ccggcagaaa gaggtaaatt tatggcattt tttaattccg cagttactac
    16261 tttgcagact atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg gcatcaatct
    16321 gttggaggga tacgggcagg acaatccggg ggcaaaatcg cagggtgtca agcagcttgt
    16381 cgctggcggc ggcgtggccc tgatcggtgt cactctggtt cccctgctgt ccgggctgct
    16441 gggctgatcc tggcctttgt ccctgtccaa acccttaact aatcgcctcc gcgtcctccc
    16501 ggccagaccg ggagggcgcg ggaaaggagg tgctacccgt gattagtgaa atcattgaag
    16561 aatggataaa aggcatcctc atggacggta tcacgggcaa cctgtccggg ctgttcgata
    16621 cggtaaatgc caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg caggcatgga
    16681 acagcggtat tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc cccatagccg
    16741 gggccatact cgcccttgtc atgtgctatg agcttatcca gatgataacg gaaaagaaca
    16801 atatgcacga ctttgacagc tccatgttct tccgctggat attcaaatcc gcgtttgcaa
    16861 tcctgatcgt aacgaacaca tggaacatcg tcatgggcgt attcgacgcc acccaaagcg
    16921 tcgtcaatca aagcgccggt gtgattatcg ggcagacatc cattgatttt aacaccctga
    16981 taccagacct tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg gggctgtggt
    17041 tccaaaccct tgttgtgggc ctcaccatga atatcctttc catctgcatt ttccttgtga
    17101 cctatggccg catggtggaa atctatgttg tcacgtcgct ggggcctatc ccccttgcca
    17161 caatgggcaa cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc ctgctggcgc
    17221 tgggcttcca agccttttta atcatggttg tggtgggtat ctacgcggtg ctgatacaga
    17281 atatcggttc cgcttccgac atatccggcg caatctgggg ctgtatgggc tatacggtgc
    17341 tgctgtgctt ctgcctgttc aagaccggca gtatatcaaa ggccgtattc ggggcgcatt
    17401 gatcccaaaa tccaagcata cacaggagga acttgtttat ttcaacgtac aacatcattt
    17461 acgctgaccc gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg gaaaatcact
    17521 attccaccat gagcattgac gagctgtgcg ccctgccggt ggaacggctg gcgtccaaag
    17581 actgcctgct gtttttgtgg gccacattcc ccatgctgcc ggaagctctg cggttaatca
    17641 aagcgtgggg cttttcattc aagaccgtgg cttttgtatg gctgaaacag aaccgtaaaa
    17701 gccttacatg gttttacggg ctgggccgct ggacaagggg aaacgcggaa atctgcctgc
    17761 tggcaacaca cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt attatcagcc
    17821 cgattgaaca acacagcaaa aagccggatg tgacccgtga aaaaatcgta gagctggcgg
    17881 gcgatttgcc ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg gacgtgtggg
    17941 ggaacgaggt ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat ggcttatgta
    18001 accgtcccca aggacttaac ccatgtgaaa agcaaggttg tgtttgggct taccaaacgg
    18061 caactgatct gtttttccgg ggcgctggtg atcggcgtcc ccttatattt tctcacccgt
    18121 gactatctta ccaacagcgc ggcggccctg ctcatggtat ttgccatgct gccgggcttt
    18181 ttgcttgcca tgtacgagcg tcacggccag ccccttgaag tggtgattat gcagatcatc
    18241 cagtgctgct tcatccagcc aaaggagcgt ccctatcaga ccgacaacac ctacaccgcc
    18301 cttgtgcggc aataccagat ggaacaggag gtaaaggcca ttgtccaaaa aggaaatccc
    18361 aagagaaacc gaaaaaacaa agctgacccg cgcccagcga aaagaaattg acgcggtaat
    18421 ccgtaaatat aagggcgacg gcaggcccca cacggcgcag cagagcatcc cctacgaggt
    18481 catgtacccg gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt gcattgagtt
    18541 tgcggacatc agctatcagc ttgcccagcc ggacacgcag acggctatct ttgaaaaact
    18601 gtgcgacctg tataactatg tggacgcctc cattcatatc cagttttctt tcctcaatcg
    18661 gaaagttgac ccggtgcagt acgccaagag ctttgagatc gcgccgcagg gggacgactt
    18721 cgacgacatc cgggccgagt acaccggcat tctgcaaaag cagcttgcaa acggcaacaa
    18781 cggcatggta aagaccaaat acctgacatt caccatcgag gccgaaagcg tgaaagcggc
    18841 gcgagccaga ttgaagcgca tcggctttga cctgttaggc tatttcaaga gcatgggggc
    18901 cgtagcccat gtgatggacg gctgggaacg gctgaacctg ctgcatggcg tgtaccaccc
    18961 ggacggcgag atattcaatt ttgactggaa gtggctggcc ccgtccggcc tctccaccaa
    19021 ggattttatt gccccgtcct ccctgtgctt cggcaatgca aagacattcg gcatgggcgg
    19081 caaatacggc gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg acgatatgct
    19141 ggccgacttc ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc aggccattga
    19201 acagaccaag gcgatcaaga ccatcaagcg caaaattacc gaccttgacg ctatgaagat
    19261 tgcggagcag aaaaaggccg tccgcagcgg ttacgatatg gacattcttc ccagcgacct
    19321 tgccacctac ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc gcaatgaacg
    19381 gctgttccag ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa agctgaataa
    19441 cgacgtgttc caagccgcag gcgtggccca aaagcataac tgtccccttg tccggctgga
    19501 ctaccagcag gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc agatcaagat
    19561 acagcgcagc cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga cgcaggagct
    19621 ttttcagggc ggcgcggcca tgtattacgg catcaacgcc aagtcccgca acatgattat
    19681 gctggacagg aagcaggccc gttgccccaa tgccttgaag ctcggcaccc ccggcagcgg
    19741 caaatccatg agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc ctgacgatat
    19801 ttttatttca gacccggagg ccgaatatta cccccttgtc aaacggctcc acggacaggt
    19861 aatccggctg tcgcctacca gcaaggactt tgtgaacccg ctggacatca acctgaatta
    19921 ttcggaggac gataacccgc tggccctgaa atccgacttt gtgctgtcgt tctgtgagct
    19981 tgtcatgggc ggcaagaacg ggctggaagc cattgaaaag accgtgatag accgggctgt
    20041 gcgcgtgatc taccggccct atctggcaga cccccgcccg gagaatatgc cgatcctgtc
    20101 cgacctccac aaagcgttgt tagatcagca tgtcccggag gctgaccggg tggcgcaggc
    20161 ccttgacctg tatgtgtccg gctccctgaa cgtatttaac caccgtacca acgtggacat
    20221 cggcaaccgg cttgtgtcct ttgacattaa ggagctgggc aagcagctca aaaagctggg
    20281 gatgctcatt gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa gccaaggaaa
    20341 ggccacatgg tattttgccg acgagttcca tttgcttttg aaagaggaac agacggcggc
    20401 ctattctgcg gagatttgga agcggtttag gaaatggggc ggcattccaa caggtgccac
    20461 ccagaacgtc aaagacctgc tttcctcccc ggaaattgaa aatatccttg aaaacagcga
    20521 cttcattacg ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg aacgcttgaa
    20581 tttgtccacc gaacagcaga agtacatcga caattccgaa cccggcgagg gccttttgat
    20641 ctttgaaaac gtggtgctgc cctttaccaa ccccatcccg cacaacaccc agctttacaa
    20701 gattatgacg acccgcttaa acgaggtggc gggagtatga cccggcgacg ccacaaagga
    20761 ggtgaaccac catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc atgacccgcg
    20821 acgggctgac gctggaaaat caggccaccg gcgaggtgga gaacatttcc agccgggagg
    20881 ccgaacagga tttgtccgcg ccggggccgg gcggcaccgc cgataagctg ctggaacggg
    20941 ccgacgccat ccatgacggc cataaggcca agaaagccgc gaagcaggcc ggggctgtgg
    21001 ccgccgaggg ggacgctgcc ctgcaccggc catccgcccg gctccaactg tcggaggaag
    21061 aacgggccga cccggattta cagccctata tccgaaaatc ggacaaacgg gccgataagc
    21121 tggataaggc ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa accgtctttg
    21181 acgaggcaag cggcaaagca aagaccgtcc tgcactttga caaacaggac aaggggcctc
    21241 cctccctgaa acccaatccg gcaggccgtc ctgtttccga ggtttttcta ttcggccacg
    21301 ggaaaatcca tgaagtcgag catgaaaacg tgggtgtgga gggcggccat aagggggagg
    21361 aacttgccga gcggcaggcc ggaaaagcca tccgcagcac catccggcac cacaggctga
    21421 aaccctactg ggacgcggaa aaggccgagc gcaggctatt gtctgccaac gcggagtatt
    21481 tctaccagaa atctttgcgg gataacccgg agatcgcaca ggcggccagc aaccccattt
    21541 cccggttctg gcagaaacag cagatcaagc gccagtacgc caaggccgcc cggacagcgg
    21601 gccagaccgc aggcgcggcg gcacagggcg cggccaagac agccggggcc tccacgaaag
    21661 cggccaaggc cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc cgccactgga
    21721 aaggggcgct ggccgtcctt gtgttggggc ttttgatgct gcttatcatc ggcggcttac
    21781 agtcctgcac cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc tcttactttt
    21841 ccgaggatag cgatatgttg gccgccgagg acgcctacgc ggagctggaa gccgagctgc
    21901 aagactatct ggacaactac gaggccaccc atgactatga cgaataccat tttgaccttg
    21961 acgagatcag ccatgacccc tatgtgctga tttccattct gtcggcgctc catgacggga
    22021 tatttaccat cggggaggta cagggtgatt tgtccatgct ctttgacaag caatatatcc
    22081 tgacagaaaa cgtcgtggta gaaacccgtt accgcaccga aaccgataca tggacggacg
    22141 cggagggcaa cacccataca gacacctacc aagtgccgta tgactattac atctgctacg
    22201 tcacgctgga aaacttcgac ctgtcccatg tgccagtcta tatcatggac gaggaaacgc
    22261 taagtttcta ttccctgtat atgtccacgc taggaaaccg gcctgatctg ttttccggca
    22321 gccagtaccc caacgcctcc accctgacgc cgccgaccta ctacgacgta cccccggagg
    22381 ctttggagga tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat atcggttatc
    22441 cctatgtgtg gggcggcagc tctcccagca ccagctttga ttgcagcggc ttcatttcgt
    22501 gggtaatcaa tcatagtgga tggaacgtgg gacggctggg ggcgcagggc ctatataaca
    22561 tctgcacccc tgtttccagt tcgcaggcca agcccggcga ccttgtattt ttcaagggaa
    22621 cctacgacac ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat gtgatgctgc
    22681 attgcgggaa cccgatttcc tacacgaacc ttacttcaaa ctactggcaa tcccattttt
    22741 acagttacgg gcgtttacct taacgccaga aaggagcttt caattatggc aaccaaactt
    22801 gaccgcatcg aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt tcagaaacag
    22861 cttcgggagc tggaaacgca gaaaaccgaa caggagaatt tgcagatcat ccagcttgtg
    22921 cgcggcatga acatgaagcc ggaggaattt gccgcgttct tacgcagcgg cgcaatgcag
    22981 gccgcccctg ccgcaacccc ttaccatgaa caggaggaca atgcccatga agaataagac
    23041 cagacggatt tcccgtattc tccccgcact tctggccgtg atgctctgcg tcacggcctt
    23101 tcccgttacc gcccttgcag gcggcaatga cccggcaccg gccccgctgc cggaggctac
    23161 aaccgaaccc gctacgggcg gtttggagcc tgaaacagac gaaaccggca ccgccacgga
    23221 cgggacagaa accgaggccg aaaaagatac cggccctctg accggcaagg acatcaccga
    23281 cctgttttcc gcgctgttcg gttccaaggt gagcattgcc gccacggacg acggcatcca
    23341 gatcaccacc ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca ccaccaacgg
    23401 cggcaggctg aatgtccgca ccggcgcggg gcttgacaag accgcgttca cccagcttcc
    23461 caatgggacg acggttgagg tagtcggcac agacggcgac tggattaaaa ttctcttgcc
    23521 ggagcgcatc ggctatgtcc attccgacta tatgaccgtc agcgaaaagg aagtagcggc
    23581 taccggcgag ggcggttttt ccctctccat tgacccggag gaaattgctt ctctgctgga
    23641 actgttcaat ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc tgtcactgat
    23701 tgacgacatc ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg aaaccaaaaa
    23761 cggcaatgtg ttttatctga ttattgaccg ggacgacgag ggaaaagaaa ctgtccattt
    23821 cttaaatcag gtggatgaag ccgatctgct gacgctgatg gaggacgggg aaaccgtcac
    23881 ccccgctgct gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga acacgaattg
    23941 cccggtatgt aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg aaccccagcc
    24001 ggaggaaccc gaagccccgg aggaagaacc caagggcggc gcgggcggcc tgatcgtgtt
    24061 cctgctggtg gcgctggcgg gcggcggtgc ggccctttac tatttcaagt tcaaaaagcc
    24121 caaggctgaa accaccggca gcgacgattt gggcgagtat gattttgggg aggacgagga
    24181 tttggaggac gaggaacccg aaaccgagtt tgacccggaa gctgattttg tgagtgaaca
    24241 ggaggacgag gaatgacata taaacttgta atcgcggaaa aacccagcgt ggcccgcagc
    24301 attgcgggcg tgatcggggc cacggagaaa cacgacggct atttggaggg gaacggctat
    24361 ctggtgagct ggtgcatcgg acatctggtg tcctttgccg acgccggacg ctatgacgag
    24421 cgtttcaaaa aatggcgcta tgaggatttg cccattctcc cggaaacatg gcagtacatc
    24481 gtccccgacg acaagaaaca gcagtttgac gttctctgtt cgctgatgca gcgccccgac
    24541 gtgaccggcc ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat tttccgtttc
    24601 gtctaccaga tggcgggatg ccaaaagccc tttgagcgcc tttggatttc ctctatggag
    24661 gacagtgcta ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta tgatccgctg
    24721 tatcaatcgg ccctgtgccg ggccaaggcc gactggctga tcggcattaa cgccacccgg
    24781 cttttttccg tcctgtacca caagaacctg actgtgggcc gcgtccagac gccgacctta
    24841 aaaatgctgg tggacaggga cgccaagatt accgatttca agaaagagaa atatcacatc
    24901 gtccatatca cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga cgctgccgag
    24961 gcaaacaccg tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc cgtgacgcgg
    25021 gagaaaaaga cggaacagcc gcccaagctc tatgacctta ccaccttgca gcgggaggct
    25081 aaccggcttt tcggctatac cgccaagcag acccttgact atgcccaggc actgtatcta
    25141 aagaagctgc tgacctatcc gagaacggat agtcaatatc ttaccgacga tatgctgccc
    25201 gccgtggaaa gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa gggcctgaac
    25261 ctttccccgc agtttgaccg catcctgaac agcaaaaagg tgtccgacca ccacgccatt
    25321 atccccaccg ctgaatttgt gaagcagggc tttggcactc tggccgagag tgaaaaaaag
    25381 ctgctgtccc tgatctgctg caagctctta tgtgccgtgg ccgggccgca tatctatgag
    25441 gccgtcaccg ccacatttac ctgtgccggg caggagttta ccgccaaggg caagaccgtc
    25501 ctttcccccg gctggaaaga gattgaacgc cggttccgtt cttccttaaa gaccgacgcc
    25561 gacgaggacg ccgaggcggt gcgggagctg ccggagcttt gcgagggcca gacctttgca
    25621 gacgtggccg cctctgtcac cgagcatttc accacgccgc ccaagcccta caccgaagat
    25681 acgcttttgt ccgctatgga acgggccggg gccgaggata tgccggagga tgccgagcgc
    25741 aagggattag gcacaccggc cacccgcgcc gccattctgg aaaagctggt gcagatgggc
    25801 tttgtccaga gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa tctggctgtg
    25861 gtgctgccgg aggcgcttac ttctccccag cttactgccg aatgggaaaa ccggctgacc
    25921 gagatcgcca aggggcaggc cgatcctgac gaatttatgg acggcatcga gacgcaggcg
    25981 cgggagctgg tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt gtttcaggcc
    26041 gagcgtgttc ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga gggcaagaaa
    26101 aactactact gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga ccgatttttt
    26161 gaggaacgca agaaagcgtt caccccgaag atcgccgctg cgctgctcaa aaccggcaaa
    26221 gtcaaggtaa agggcctcta ttccgtaaag accggcaaaa cctacgacgg gacggtgctt
    26281 ttggctgata ccgggggcaa gtacgtcaac taccgcattg aacacaggag ctaaaggaca
    26341 gtaacacagc aagcataggg agtgtggcta cactcccggt aaaatccccc ggccgcgtcc
    26401 ctgccgggcc ggggattttt gcttgttggg gaagtcccca accccctatt ttttgaaaca
    26461 gaacagagaa aggagtgacc gcgtgaacga agtattttct atccaagtcc ataaccggca
    26521 gtttttggaa cagggcaaac aaggcgtctg gctggatttg cccaccacaa ccgaaacgct
    26581 gcaagccgcc ctgcgggatg ttggcattac tgccgacaat ccgcaggatt tttttatcaa
    26641 cgggtattcc tgcccggagg acagacacct tgccattccc tatgacatgg tgctggccgc
    26701 cgacgtggac gagctgaatt tccttgccgc ccggctgggc cagcttgacg ccgccgagat
    26761 cgcagagctg aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata tcggccagat
    26821 catcgactac accgaaaatg tggattacta tgtgcatctt cccgacgtta ggggggatgg
    26881 acagttgggc gactactacc tgaaccgttc cggcatggtg gatatgccgg aggaatggaa
    26941 agccgggatt gatacggcgc agtttggacg ccacattgcc cagcaggaac aaggcgtttt
    27001 tacccagtac ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc atgagggcca
    27061 gccggtgccg gaggaatacc gggtgatgca ctacccccag ccggaagctg accgggagga
    27121 accgcaggcc cagcagccgg aggcatccga aagccccgac aaagcgccgc ccccggttat
    27181 cccgatcatg ctggacggca tgaacagcaa agaacgcatg aaagtgatta ctgaccgtct
    27241 ggaaaccggc gtacaggagc tttttgaaag tagccgctat atggcttact tatccgctat
    27301 gtccaagatg cacgattaca gtctgaacaa tacgctgctg atcgccatgc agggcggttc
    27361 gattgtggcg ggctataaca ggtggcagga tgaatttgac cgccatgtga agcggggcga
    27421 gaaaggcatt aagatactgg ccccggcccc gttcaaggtt aagcagcaga tggagaaaat
    27481 cgacccggcc acccagcagc ccgtgatcgg cgcggacggc aagcccgtga ccgaagaaca
    27541 ggaagtgacg gttccggcct ttaaggtggt gacagtcttt gacatcagcc agaccgaggg
    27601 caaggatttt cccatgccct atatcagtga acttaccggc gacgtgcagc agtatcagga
    27661 tttctttgcc gcgctggaac ggacatcacc ctatcccata agcattgagc cgatttcaga
    27721 cggggccaag ggccgctgct tctacgcaaa acagcgcatt gccatcaacg agggcatgag
    27781 cgaattgcag accctaaaga ccgccatcca tgagattgcc catgccaagc tccacgccat
    27841 tgaccgggag gcccccgccg aggaacaggc ggcccggcca gaccgcagca cccgcgaggt
    27901 acaggccgaa agcgtggcct ataccgtctg ccagtattac gggctggaca cgtcggacta
    27961 ttcttttggc tatgtggccg attggagcag cggcagggag ttatccgagc tgaaagcctc
    28021 ccttgaaacc atccgggcca cggccagcga attgataacc aagatcgacg gccattttgc
    28081 ccagcttcag caggagcgcg aggcccggca ggaacaggag gccgtcacgg ttgacgttac
    28141 cccggaggca gcggccccgg cgcaggccga ggaagaacag gccccggccc ttgatccggc
    28201 agccgagccg cttgtgactg tgctatggag cgaaagcccc cagctaaagg acggccagca
    28261 aatgccgttg catgaggccg acgcgctgtt tgcccgtctg gatgcagcat atccggcaga
    28321 ggcaggctat gacaaaaccg cgttccgcat tgactgtatc tttcagggcg agccggacag
    28381 ctacgagggg cggcaggatt ttggagatag ggacggttcc ctgatcgaac atatccagag
    28441 ctaccaccaa ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg ctacaaccgg
    28501 catagagggc tgggagcagg acaaggccca tcgggatatg ctgttaggcg agtttgtccc
    28561 ctatatgaag ctgcattgca gccttgccca aatggaacag acggcgcagg gccgcttaca
    28621 gtccggggaa accctgacgc cggaggaaac ggcctatttg ggggcgcttc tctcttatgt
    28681 gcaggagtgc cgccccatgc tcaatcaggg ccagtatcag ttgccggagc cgccgaaact
    28741 ggctgacttc gaccagagct tgcaggacta caaagcacag gtacaggccg agatcgcgca
    28801 ggaggccgcc gcagccggta tgaccgtgga ggaatacgcg gcggctggct atgaagcacc
    28861 ccaacaggac accttttcca tctaccagct tcgaggcggg gaggaaaccc gcgactatcg
    28921 gtttgagcca tacgagcgtt tacaggccgc agggctggcc gtggacagtg ccaactatga
    28981 ccttgtttat acggcaccgc tggataaaag cacgtctttg gaggatattt accgcacatt
    29041 caacatcgac cacccggcag attttagggg acacagcctt tccgtttccg acgtggtggt
    29101 gctgcaccag aatggacagg acaccgccca ctatgtagac agcttcggct atatggaagt
    29161 gccggagttt ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg agcagaacga
    29221 aaacatgatt gacggcatta tcaacaatac gccgcccgaa ccgcagccgg aggaacccga
    29281 acagctatct tttgaggatg tggcagttgc ttccggccag acacaggagg aacccccgga
    29341 ggctgcgccc ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag ccgcccgccg
    29401 cgccaaggaa gccatgagct tttctgacta ccggcccggc agcgccacgg cggaataccg
    29461 gcactatgtc gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg tggagccaga
    29521 gtatcacgaa aagatagaca gcctccttga cacctacgcc cgcaagctgg ccgagaacat
    29581 gaataagggc tatgagatta ccgcccgcgt cccctctgtc ctgattgccg ggccgtccaa
    29641 tttcccggta cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca tggaggaatt
    29701 tcagtacatt cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg gtatcagcgc
    29761 cgacgatccg caggccgttt ccaagctgga aaagaaactg gaaaagctgg aagcgtcgca
    29821 ggagttgatg aaggccgtca atgcctacta ccgcaagcat gggacgctgg acggatgccc
    29881 ccatctgacc gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg gctggcacta
    29941 tgagaaaaaa ccgttccagt cctggcagct ctccaacaat aacgccgaga tacgccggtt
    30001 aaagggccgc attgaggaat tgacccgcca aaaggaggcg gcctatgtgg gatgggagtt
    30061 tgacggcggc actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct tctttgaggg
    30121 gaagccggat gctgctgtac gcgacgagct gaaaagcaac ggtttccgct ggtcgcccaa
    30181 ggccggggca tggcagcggc agttaaacga caccacaatc cgcgtcgctg accgtatcaa
    30241 gtgcatccag cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg cccgcaggga
    30301 gaaagaaaag ccctctatcc gggcgcagct caacgcggcc aagaccgaac aggccaagca
    30361 gccaccggcg cgggaaaaat caaagggatt ggaggtagag taaatgcaga agtttgaaaa
    30421 tgtggacgtt ctggccgcgc tggaacagat catgcggcag aacaccgcgt tttatcaaag
    30481 tgactttggc attgacaaag acattatcag gcgggatgcc gccagcgatc aggccgcaga
    30541 caagaccctg ctctggatgt cccggccctc cgggacgtac tgtttccggg aacgtgatgt
    30601 tttcctgaaa gacacccgac agtacaacac atggaaattt tacggggagc agacccgtga
    30661 caagatactg gcctacgcgg tggagctgac cggcacacag ggcggcacta tccggggcaa
    30721 tctctatgag ctggactatc cgcagcattt ccggcacgtc attgaaaccg cccagccggt
    30781 gagtgtgaac cggctctttt atgagcatgg cacccgtgac atcccggaga gccagcgttt
    30841 tgacggaagc cctgaccggg tattgggaaa ctttttgtac tatgaggccc agccccatga
    30901 cccggctgtg ctgcaggagg cgctacggac ggagcagcac agccgggggc gggccgtccc
    30961 cggcgatttc aaagtccacg tcaccgcttt gcatgacagc cggattgaaa ccgaggcccg
    31021 gcgtattgtg gacagcctga aagatctgtc cgcgcctaac agccccaata aaacgcattt
    31081 catggtggag atctccccgc actttgcggc gattgcttcc accaaggata atgaccggct
    31141 ctttgccatg ctgccctata aatccctgtg ttttaccggg atgaaaggca ggcatggcgt
    31201 gtatgccgtg attaaccggg acgagcgccg ggatgtgagt attcgcaggc cccggccctc
    31261 tatccgcaag cagctatccg acagcaaaca ggccgccagc ccaaaaaagg cggcggccag
    31321 aaccaaaaag aatgaattgg aggtataagg aatgatgcac tttaccgtgg aggaagaaaa
    31381 cctgatctgt atgtaccaca atgcagaccg gcgcagaacg ataaacaggc tcaccgccgt
    31441 catgccggtt atggatgggg atatgcgggc gcaggcccag cagacccttt ccaagctgga
    31501 acgcatgacg gatgccgact atgacggcca gaagttccac ttcacagacg aataacgaac
    31561 agcagacgcc gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt tctttttgcc
    31621 tattcctacg catttagaac ccggtattgt cggggatagg atgaagtatt cgcttgcccc
    31681 tgcaaacatg cgggagaagc cttgcagcgg gcggttttca atcccttaaa tgcgtaggtt
    31741 gcagctataa ttagacccct gaaacagata tagccgcgca gcgggtgtat ctgtttcagg
    31801 ggtgtgcagg tgatagccgc aaagcggcgt aagagggtgc agccctcttg ccgtctatct
    31861 ctgttccagc gggaagctcg gcaatgcttc cagcagtttc agggaaatgc gctgccgtat
    31921 atcttcgtca atgactttct tctttttgcc gtttgcctgt ctgatctcca ccgtggaaag
    31981 ctcgttgatg tagtcagcat aatgggccaa cactttttcc gtggcccacc tctcaccggc
    32041 aacggcggct ttgatcgttt catagtccag cagcttttct tgtccgtcca tcctgatacc
    32101 cccgtcagac tgattttttc tatgatacca tattcggaag catggaacca gagaaaacgg
    32161 cgcttttctg ctggtaaatt ctacgcattt agaacagcac ttcggcgggg ataggataaa
    32221 acacccacat accccgctga ccatgccgga aaagccttga aatataaggg cttttacagc
    32281 tctaagtgcg taggtttcca gtatcttttc cgctggcgct cggcctcttt gcggcggcgc
    32341 atccgtttgg agcaggccgc gcagtatttg gcccggttag acttcggcgt gaacgtggcc
    32401 ccgcactcac agcagcgttt cacttcgtcc cggtctttgg tgatctcggc atacagggcc
    32461 gcatccagcg ggaggacagc ggccttaaac cagcggcaca gaagcgaata ggaaatgctc
    32521 tgtacgcaaa tacattcttc gccgtcgtcc agcaacaggc agttgccgtc acaatagttg
    32581 cagcactcat gcgtcagctt ccgggccttg cgatattgag ggtaattcat tcgtggaata
    32641 ctcatagctc ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg tccacattct
    32701 gcttgatgat gccgtattcc tgcatctgct ttttcgcttc gccgtacttc tcatacagcc
    32761 gttccttttc ctcggccaat cgcttgtact cggctttcag ggcgtacaga tcggggagct
    32821 ttttcacacc ctgtcccttt agggcctttg ccgcagcttc atacaaaatc aacgtgcttt
    32881 catgctcacg ccggaatttt gccttatccg ctgcattgcg gtattccata gccacgggcc
    32941 agagctgctt gtaggtagaa atattcttta tcagcaccgc catatccgca agccggtgtt
    33001 ccatagcttt gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg gccactttgc
    33061 tttccaaatc cgggtaggtg tctatcccat gttcagtcag gaaattcaaa gtctttgcgg
    33121 cctgcttcaa attgtggagc ttcgcccacc tctcatagcc tgccgactgc tgggccttaa
    33181 tgttgttttc cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc gctctggtga
    33241 taacgcggcc tttgatacgc tctttgatgg cttcctcggt gtaggcttcc cccaatgtct
    33301 tgcagcgggt aaagcgttcc tgtccgggag cgcggaaaga aataaacttt cccggcttga
    33361 tctcatagcc ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt gcctgcggga
    33421 tagcggcgtc aatggctgct ttgagcttcg ctttccagct cgtccctttc cgctgggcgt
    33481 cccactcggc atagctcttg cccttactct ggccgggctt caccacggac aatccgtttt
    33541 ccttgcacag cctgtcgctg gtgcgccgga tataggcata gctctgtttg ttggaaatgt
    33601 acttgcgctg atttaccata tccacggcac agaaaataat gtgattgtgg acgtggccct
    33661 tatcaatatg ggtagtcagc acatagggat atttcccttg cagcacttcc tcggcaagct
    33721 gcttcccgat ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag gattgtatca
    33781 ggtggtggcc cagattattg cccttttggt atgcctgcgc cagcagcatt tcaaattcaa
    33841 tgtccgctgt ttcataagag caggcaaagg aggccaccag cagcttgccg tcagttttgt
    33901 ccgggttctc gatgtagtca agggcttttt tcagcgttga cttgatagga tgtatctttg
    33961 taactgccat atctgcgcca gcgccccctt tatatcctcg atgtcctgtg cgtacaccgg
    34021 ccccataccg ttcagcctct ttgcaatctg attgacgttg acgccgatct tctgtatctc
    34081 ggcggtctgc gcccggatgt cgctggtgtc catgttgacg atatagccgt caatgagcat
    34141 cttccgggcg taggctgaaa agttcttcgt atgcagcagc gccattttct gttcaatcat
    34201 agcccgttcc tccggcgtca cagggacgcg cagcacgatt tttcgctttc tgtttgccat
    34261 gcttcaccgt cctttccatt caggggtttg gggatttccc caacaagcaa aacggacgcg
    34321 gcccggcagg gacggggcag gccgtttttc cgggagtggc tacactccct atgcttgcta
    34381 cgttactgtt tctccactaa caagacgttt ccaaagcccc aaactacccg caaaatagcc
    34441 ctctggaaag aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt
    34501 aaaacagagg atgccagtta cacatcctcc gtttcattct tcttcggctt ttcaattagt
    34561 atggcgctgt caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca gagtttcagc
    34621 ttgtggctga ccctttgccg cagttcgctt tcctcccgca tgatctccgg gttaaagtag
    34681 cgttccacag gaagcccgca ggctttaata agctgtatga caaccggcag actgggaatg
    34741 gccccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt ctctgccatt
    34801 gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc gaaagtttca
    34861 aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt gtatatttca
    34921 tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt tcacattttg
    34981 tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt actatggact
    35041 acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg ataaactact
    35101 attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg ctcattccca
    35161 aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt actgaatagt
    35221 ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat ggtattttgt
    35281 cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa aagtattctc
    35341 atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa ctttatcatg
    35401 acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc agccgtatat
    35461 ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag acattggata
    35521 actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg gggaggtacg
    35581 gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt cctgtaactg
    35641 caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct actgtcttga
    35701 acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga gggagtacac
    35761 acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg
    35821 cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa
    35881 gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag tattatcggg
    35941 atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt taattattat
    36001 tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga aaagaagcga
    36061 gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact acagggaaac
    36121 tacagactgg ctacagggaa agtacagatg cgcttgccat aataacaatc gtaccagcca
    36181 caaatcgtag ttttattgca aaggaggcat tcgatcaaat ggaaaaaagc aactatcatt
    36241 ccaatgcgga tcatcacaaa cggcatatga aacaatccgt ggaaaagcgg gcttttctat
    36301 gtgcgttcat tatttcgttc acagtctgca cgctgttttt ggggtggaga ctggcttccg
    36361 tattggaggc aacacagata ccgcccatcc ctgcaactca tacaggcagc agcactgacg
    36421 tagtggagaa tttggaggaa aacgctcttg ccaccgccaa agaacaggga gatgaacagg
    36481 aatggagcct gattttagtg aacaggcaga accccatccc cgcacagtac gatgtggagc
    36541 ttgagcaact atcaaatggt gagcggatag atattcggat ttctccctat cttcaagatt
    36601 tgtttgatgc cgcaagaact gatggagttt acccgattgt cgcatccgga taccgaacaa
    36661 cagaaaaaca gcaagaaatt atggatgaaa aaattgccga atataaggcg aaaggctaca
    36721 cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga acgagcgagc
    36781 atcagcttgg tcttgctgtg gatatcaatg cggacggaat tcattcaaca ggcaacgagg
    36841 tttatagatg gctggatgaa aacagctatc gttttggttt tattcgtcgc tacccaccag
    36901 acaagacaga gataaccggt gtgagcaacg agccgtggca ttaccgatat gttggcatcg
    36961 aagctgccac agagatgtac aaccaagggg tttgccttga ggaatattta aaaccagaaa
    37021 aatgagaaaa ggatgttatg ttatggacag aaaaagattg acacagcgtt tcccgttccc
    37081 gcttccaatg agacgggcgc agagaaaaat gtgcttttat gcgggaatga gatttgacgg
    37141 ctgtcgctat gcgcagacga taggggaaaa atcgctttcc catttgctct ttgaaacgga
    37201 ttgcgcatta tacaaccaca ataccggatt tgacatgata taccaggaaa acaaggtgtt
    37261 caacttaaag ctggcagcaa agaccttaaa cggcttattg ataagaccgg gggaaacctt
    37321 ttctttctgg tggctggtac gccatgcgga caaagatacc ccctataaag acggccttac
    37381 ggtgaccaat ggtaaactca ccaccatgtc gggcggcggt atgtgccaga tgagcaattt
    37441 actattttgg atgttcctgc atacgccatt gacaattatc cagcgcagag gtcacgaagt
    37501 aaaggagttt ccagagccaa acagtgacga gattaaaggg gtggatgcaa ccatttcaga
    37561 gggctggatt gatttaaaag tgcgaaacga taccgactgc acctaccaaa tatgggtgac
    37621 cttagatgat gagaaaatca tcggtcaggt gtccgccgac aaagagcccc aagcattata
    37681 caaaattaca aatggcagta tccagtatgt ccgtgaaagt ggcgggattt atgaatatgc
    37741 ccaggttaaa cggatgcaag ttgccttagg taccggggaa ataatagatt gcaagctgct
    37801 ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa agtgtggata ttcaggagga
    37861 gaaccaatga gaaaaagtgt gggcattact gtttttggat gcgaacagga tgaggcaaat
    37921 gctttccgcg ctttatcgcc ggattttcat attatcccta cgctgattag cgatgcgata
    37981 tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc
    38041 gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg gggtaaaata catttctacc
    38101 cgcagcatcg gctgcgatca cattgatacg actgccgccg agagaatggg aatctcggtt
    38161 ggcacggttg cgtattcgcc ggacagcgtt gcggattatg ctttgatgct gatgctgatg
    38221 gccatacggg gtgcaaaacc caccatgcac gccgtggcgc aacaagattt cagattggat
    38281 cgtatccggg ggaaagaact gggggatatg actgtgggag ttattggaac cggccatatc
    38341 gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc atgtgctggc ctatgataac
    38401 agccgaaaaa tggatgcaga ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt
    38461 gttacgctcc atgtgccgct ttgtgcggat acccgccatc tgatcggtca gaagcaaatt
    38521 ggagagatga agcaaggcgc atttttaatc aacaccgggc gcggagcgct tgtcgatacc
    38581 ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg
    38641 gagggcgagg atcagtttgt ttataccgat tgctcgcaga aagtgcttga ccatcccttt
    38701 ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac cccatacggc gtactacact
    38761 gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga attgtctcaa ttttgaaagg
    38821 agtttacagc atgaatagaa taaaagtcgc aatcatcttc ggcggttgct cggaggaaca
    38881 tgatgtgtcg gtaaaatccg caatagaaat tgctgcgaac attgatacgg aaaaattcga
    38941 tccgcactac atcggaatta caaaaaacgg tgtatggaag ctatgcaaga agccatgtac
    39001 ggaatgggaa gccgacagtc tccccgccat actctccccg gataggaaaa cgcatgggct
    39061 gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt gatgtggctt tcccggtttt
    39121 gcatggcaaa tgcggggagg atggtgcgat acaggggctg tttgtattgt ctggtatccc
    39181 ctatgtgggc tgtgatattc aaagctccgc agtttgcatg gacaaatcac tggcctacat
    39241 tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa atgattgata aaggtgacaa
    39301 gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt caggttcgtc
    39361 ctttggcgta accaaagtaa acggtacgga agaacttaac gctgcgatag aagcggcagg
    39421 acaatatgat ggaaaaatct taattgagca agcgatttcg ggctgtgagg tcgggtgtgc
    39481 ggtcatgggg aacgaggatg atttgattgt cggcgaagtg gatcaaatcc ggctgagcca
    39541 cggtatcttc cgcatccatc aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat
    39601 tacagttccc gcagacattc cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa
    39661 agtatatcgg gtgcttggat gcagagggct tgcccgtgtt gatctttttt tgcaggagga
    39721 tggcggcatc gttctaaatg aggtcaatac cctgcccggt tttacatcgt acagccgcta
    39781 cccacgtatg gtggccgccg caggaatcac gcttcctgca ctgattgaca gcctgattac
    39841 attggcgtta aagaggtgat aagtatggaa aatggttttt tgtttttaga tgaaatgttg
    39901 catggcgtcc gttgggatgc caagtacgcc acatgggata acttcacagg aaaaccggtg
    39961 gatgggtatg aggtgaatcg catcatcggc acaaaggccg tggcgtttgc tctgcgcgag
    40021 gcacaaatcc atgcggctgc gcttggctat ggcttgcttt tatgggatgg atatcggcca
    40081 agaactgcgg tggactgctt cctgcgttgg gcagcgcaac cggaggacaa cctcacaaaa
    40141 gaaaaatttt accccaatat agagcgagcc gagttgatta caaagggtta tgtggcctca
    40201 caatccagcc atagccgtgg aagcgcaatt gatcttacgc tctaccacct ggatacaggg
    40261 gaacttgttt caatgggaag taacttcgat tttatggacg aacggtcgca tcatacagca
    40321 aaagggatag gggatgcaga ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc
    40381 agcggatttc agtcttatcg ctttgaatgg tggcactata agttgattga tgagccatac
    40441 cccgatacct attttaattt tgccgtttca taatgaaagt attggttttg tatcagattt
    40501 tggatttgat aaaatctatg ctggtcatgg acaatctttt actggaaagt ggttaaagca
    40561 tcttttgtaa tacattgcaa attatagtat tcaatctgcc cagcggcaga aaagaaaaaa
    40621 accgctgttg ggcagacctt tcccacgccc attttgtatt ttgctggcgg cggttttgag
    40681 aaatcaaggc cgccgttctt ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc
    40741 gctatgctgt tttgctattg ccatgctcta atccacttcc accactcaaa aaaagccttg
    40801 cccctccaca gggctattcc ggttatgagt atgtactata ccatgtaaat cagcagcata
    40861 gcaaagcatt ttctcgcccg gtaggtttgc gacctgccgg gcgatttttg tcgtcttttg
    40921 cggcccggct gtccccaaaa aaattttttg agaaattttc aaaaaggagg tatttagcgg
    40981 gtagctggcg gccttgtcaa agtcttgtta gcggaaaggg agagaaccac ctaagtcccc
    41041 cacagaaagg gggtgagaag atggatgcta tcccccgcga ctatgaagcg cggtgcatgt
    41101 tcgatgcttt ttgcaagacg gtactgcgta acgaggccaa aagctacttg gctgaaatga
    41161 agcgccgccg tgaccgtgaa gtgtcactaa gctctttgtc acaggcagat ttggacaagc
    41221 tctgtacagt agatcactac cccagcgaca gctttacgtt ttcgtcgcat ggctatgacc
    41281 tacatatcaa caatgagctt gttgccgaag cctttgccgc gctgcccagc atggagcaga
    41341 gtattttaat cctgcactgt gttctggata tggcagatgg tgagatcggc ggccttgtgg
    41401 gaatgtcccg gagtgccgtt cagcggcaca ggacaaatac cttatcggag ctgcggaaac
    41461 agctaaaggc gttgatgccg aagggaggtt agacgcggat gaagaacccg aactacaagg
    41521 gcattgagct tttgcccctt tcggtgatcg aggccgcccg cgctggggat gccggagccg
    41581 tagagcgcgt tttgcagtat tacagcggtt acatgaacaa gctgtgtacc cggacgctct
    41641 atgacgaaaa cggctatccg catgtgtgtc tggacgaggc catgaagcgc cgtctggaaa
    41701 tcaagctcat ccacgctatc gtcacgaact gacgaaccgg ccccggcagc atgaaacggc
    41761 ttaccctttc cccgtttcgg tgttttcccg gtggcctgat cgcaccttga taaatatgcc
    41821 cgcaagataa cggcggcgca tcatagggca tacggacaca ttctgtctgt gccgagccgg
    41881 acggctggtg cgccatgata cttttcccta cgggaaaagc ggagcgagga acaccacgcc
    41941 gcaatgtgag cgtggcggct tgcgggcgac gacgtacagg caggactatg atactccctt
    42001 atagccacag tccgagcgtt aaaagcgtcg caggcaatgg gtagggccgc gtgagaacca
    42061 cgcgggggtg aaagtcccgt ggagctgtgc cagcagccgt ccgtctatgc ccctaaatca
    42121 cacaaacctt tgaatttgtg aaagggggct gcattatgaa ccatgatgtg cctatttggg
    42181 aaaaatacac gctgacgatt gaggaagcgt ctaaatattt ccgtatcgga gaaaacaaat
    42241 tgcgtaagct ggccgaggaa aatccggcct ctggctgggt cattatgaat ggcaaccgca
    42301 tccagatcaa acgcaagcag tttgagaaaa taattgacac tttggacaca atctaatagt
    42361 gaaaaagggc cgcagatatg ttataataat ttataagcat atatgcggct ctttccgtca
    42421 tggaaaggag catgacgaga tgtcagaaaa aagacgagac agcaaaaatc gcgttttgcg
    42481 gtctggagag agccagagaa aagacggacg atatgcttac aaatatgttg atacctttgg
    42541 aaaaccgcag tttgtttact catggaagtt agtgcctacg gataagaccc cggcaggtaa
    42601 acgggaggat atttcactcc gggaaaagga gaaagaaatc cagaaagacc ttgacgacgg
    42661 gatagacacc atcggcaaga aaatgaccgt ctgccagctc tacgccaagc agatcaggca
    42721 ccggggcaat gtgaagcaca acaccaaact gggccgggaa cggctgatgc gtattctgga
    42781 acaagatcgg ctggggagct gcccgattga cagtgtgaag ctgtccgacg caaaggaatg
    42841 ggcgcttcgg atgaaagaaa agggcctttc ttataagacc atcaataacg acaagcgttc
    42901 cctgaaagcg gctttttaca ccgccataca ggacgattgc atccgaaaaa acccgtttga
    42961 cttccagctt agtgacgttc tggacgacga tacggaacca aaggtgcctc ttacaccagc
    43021 acaggaagaa agttttctgt cctttattca aggtgataag gtttaccaaa agcattatga
    43081 cgcaatcgtt attctgctgg ggacagggct tcgcatttcg gaactgtgcg gactgacgga
    43141 caaagatttg gattttgaaa accgggtaat catcgtgagc caccagcttt tgagaaatac
    43201 cggcgtcggt tactatattg acgaacccaa gacccaaagc ggcgtcagaa aaatccccat
    43261 gaatgaggaa gtgtatcagg cgttccagcg agtgataaaa aaccgcaaag gtgcaaagcc
    43321 gttcattatt gacgggtatg ccaatttcct gtttctcaaa cagaacggct atcccatgac
    43381 ggcggtagat tatggcggga tgtttggcag gcttgtgaag aagtacaaca agagccatga
    43441 agaagcgttg ccgaaaacga cgacgcccca tgctatgcgt catacattct gcacccggtt
    43501 agccaatgcg ggaatgaacc ccaaagcctt gcagtacatt atgggacatt ccaatatcac
    43561 catgacgctg aactattatg cacacgccac attcgattcc gcaagggcag aaatggaacg
    43621 gctggcagcc tgacacaagg ccggttttac tactccattt actacttttg agggggaaat
    43681 ggtgagaggt aataagaaca tttgtaagta tctaacaata tcaaaaatgc cggaaaagcc
    43741 cggaaacacg ggctataccg acattcaaga acttctaaaa agataatcta aaatcccata
    43801 taattttaga ataaaatttt aaaaaggcaa aaaattcgcc attttaggaa agcgttttaa
    43861 aaaagtgttg caatctttta aaaaaatgct atattaaaaa tgaaccggtt caaatacttg
    43921 tttaagtata attaatagtg agaggaagtt gctctactga agactttagt tatatggata
    43981 ttgatgacaa gcaacactat gcaaagaaag cggtcatcat aatcgaacaa caagaaagag
    44041 aaatcaaatg taactggcag gagtgagaat gtatggcatc gatccgtgac gtggctaaga
    44101 tggctggtgt ttcagtggga acagtttccc gttacttgaa tggtcagcaa ctgaaggaaa
    44161 agaacatgaa aaaaatagca gaagcgattt cagcgttgga ctataaagaa aacatcattg
    44221 ctaaagggtt gaagaataat cgaagcttct ctattggact tttgataaac gggatttcca
    44281 gccgttttgg gtcggaagtt gtctcaggga tcgaaagagt agctgaagaa aatgggtata
    44341 gcttattatt aagcgggttt gccgatcagc cagaaaaaat tgaacaaaaa attgaatacc
    44401 tgatgaagca tgtgatcgat gggctgattg tcttcttatc cgaagaagag tggtctggat
    44461 ttgaaatgct cacaaaaatg agtattccag ttatcgcatt gaattgccca gaaggaccag
    44521 caggtattga tacaatatta gtgaatgatc gagaaagtgt agcaaaagtg atcgctcatc
    44581 atggtgaaca aggacataaa aaaatcggat ttattgctgc cactcaaaca gactacgtag
    44641 caagagaacg tttagcagga gcaaaagaag cggtacaaaa tgatccatcg atccaattag
    44701 aggtctttac aggagactat tctagaaaaa gtggctatta tggggcaaaa gaattattag
    44761 aaaaaggagt aacagcgatt tttgtcagca attataatat gtcgatcggt gctatcgaat
    44821 gtttcaatca agaaggtatc agaatcggga aggatattgc gtttagtcat tatgattatt
    44881 tggataaaaa cgatttgagc atccttccga aaatcacgat tacaccacca acagataaaa
    44941 tcggggaaca agccgcagaa cagctgttga aacgaatcaa aaaagaagag gaagaaaatg
    45001 aaaaagtaat tgtgatggaa aatatcatac atggaatcaa ctaaaacgaa ctaaaaaaga
    45061 aaaagaggtg ctttgatgaa aaaatatcaa tttccagcaa atttttggtg gggttctgca
    45121 gcaagtggcc cacaaacaga aggaaggatt tcaggtgatg gaaaaggaga gaacattttt
    45181 gattattggt acaaaaaaga accgcaaaag ttctttgacc aagtaggacc tgaaaaaact
    45241 tcacaagttt atacgaagta tcaagaggat gtacagttga tgaaacagac tgggcataat
    45301 tcatttcgaa catctatcca atggagccgc ttgatacctg atgaatctgg aacaatcaac
    45361 gaagaagccg ttcgtttcta tcatcgttat tttgatgagt tgattgaaaa cggtatcgaa
    45421 ccctttgtga atctgtatca tttcgatatg ccgatgtatc tgcaagaaaa aggcggatgg
    45481 ttgaaccgag aaacggttga agcctatgaa aaatatgcca aaagttgttt tgaattgttc
    45541 ggttcgaaag taaaaaaatg gtttactcat aatgaaccaa tcgttccagt cgaagcaggt
    45601 tatttatatc gatggcatta tccagaagag tcagatatga aaaaagcgat ccaagttggc
    45661 tatcatgaag cgttggcctc agcgttagcc atcaaatctt accatgaaat ggagcaaggt
    45721 ggtgttattg gtatcatctt gaatctgaca ccaagctatc caagagacga atcaaaccct
    45781 gaagatgtta aagcagcgca aatagcagat gcgttcttca atcgttcatt ccttgatcca
    45841 gctgtaaaag gcgagtttcc aagtgaattg attgagatag taaaagaact tgatctcatg
    45901 cctgtgatcc atccagagga tttgacggtc atcaaagaaa atacagtaga tcttttaggt
    45961 atcaattatt accagccacg ccgggtaaaa gcaaaagaaa caccaatcga tactaaaaac
    46021 ggtccaatgc ctgaggatta tttcgacaat tatgaaatgc ctggtcgtaa aatgaatcct
    46081 tatcgcggat gggagatcta tgaacaagga atctatgata ttttgatcaa tgtacgagat
    46141 cattatggaa atatcgactg ctttatttct gaaaacggta tgggtgtaga aaacgaagca
    46201 cgatttatca aagaagatgg catgatccac gatgattatc ggatcgaatt tgtccaatct
    46261 catctgaaat atgtccatca agcaattcaa gaaggtgcca attgtttagg ctatcatatg
    46321 tggacatgta tggataactg gtcatggacc aatgcatata agaatcgata tggctttatt
    46381 tcagtagact tagcacaaga tggaaaaaga acaatcaaaa aatcgggtca ctggttcaaa
    46441 gacgtatcag ataaaaacgg atttgaggct taagaaagaa tagttgagag caaatgcgtg
    46501 aaattctcca ttcttaccat tttgtgaaaa agaagttcaa ttatttacga tctttttcaa
    46561 cttttttgtc tttctatttc attttttgta tataatagaa tagtcaaaat taaataggag
    46621 tggaggaaag catgaggaaa aaaagaccgt ttcttctctt ttggttattc ggttttttct
    46681 tactgcttat tttatccgca gcattttcag gaaaaatgac agcagatctt gctcagaaag
    46741 aaacagaaaa aatcgtagca agcaaagaat cagtagataa tttcaaaaaa gaattctcaa
    46801 cttattggtg ggcggagtat aaaataactg ccaattacga aaaaggcgaa ttggacatct
    46861 atttgccaaa aggaaaagaa gaaaatcaat ttattaatat cgatgacgta cttagtgcga
    46921 tttctctcag acaatatcaa tttaaagaca agcatttgac cgttttgctt ttgactcatg
    46981 atggcgacaa agtagcccgt ttgaaaaatg gaaaagtgat caccgcttat tctgaaaaag
    47041 tagacaaaaa agtggtcgcc gatcgtttag cggaatggaa aaagacaaat gaacaagtca
    47101 agaaaacaac tgtctcttct caaaaaaacg agtcgacagg aacaagtcct tcagattctg
    47161 gcagtggaca aacgataacg ggaagtgaag aaaatcaagc ctcaattatt acgaatgagt
    47221 catcacaagg acaatatcaa tcctatggac aatgaaaaa
//
LOCUS       DACGLH010000014        46226 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.015, whole genome shotgun sequence.
ACCESSION   DACGLH010000014 DACGLH010000000
VERSION     DACGLH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.015"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(13..1047)
                     /locus_tag="IXT21_000876"
     CDS             complement(13..1047)
                     /locus_tag="IXT21_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4868302.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            1162..3387
                     /locus_tag="IXT21_000877"
     CDS             1162..3387
                     /locus_tag="IXT21_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ4868303.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            3405..5288
                     /locus_tag="IXT21_000878"
     CDS             3405..5288
                     /locus_tag="IXT21_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ4868304.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            5269..6243
                     /locus_tag="IXT21_000879"
     CDS             5269..6243
                     /locus_tag="IXT21_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ4868305.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            6902..7924
                     /gene="aroF"
                     /locus_tag="IXT21_000880"
     CDS             6902..7924
                     /gene="aroF"
                     /locus_tag="IXT21_000880"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ4868306.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            7957..9021
                     /gene="aroB"
                     /locus_tag="IXT21_000881"
     CDS             7957..9021
                     /gene="aroB"
                     /locus_tag="IXT21_000881"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ4868307.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            9023..10189
                     /gene="aroC"
                     /locus_tag="IXT21_000882"
     CDS             9023..10189
                     /gene="aroC"
                     /locus_tag="IXT21_000882"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ4868308.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            10206..11297
                     /locus_tag="IXT21_000883"
     CDS             10206..11297
                     /locus_tag="IXT21_000883"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ4868309.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            11310..12605
                     /gene="aroA"
                     /locus_tag="IXT21_000884"
     CDS             11310..12605
                     /gene="aroA"
                     /locus_tag="IXT21_000884"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ4868310.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            12598..13107
                     /locus_tag="IXT21_000885"
     CDS             12598..13107
                     /locus_tag="IXT21_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ4868311.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            13104..13940
                     /gene="pheA"
                     /locus_tag="IXT21_000886"
     CDS             13104..13940
                     /gene="pheA"
                     /locus_tag="IXT21_000886"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ4868312.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14003..15739)
                     /locus_tag="IXT21_000887"
     CDS             complement(14003..15739)
                     /locus_tag="IXT21_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4868313.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(15736..16665)
                     /locus_tag="IXT21_000888"
     CDS             complement(15736..16665)
                     /locus_tag="IXT21_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4868314.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            complement(16665..17243)
                     /locus_tag="IXT21_000889"
     CDS             complement(16665..17243)
                     /locus_tag="IXT21_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ4868315.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            17516..18802
                     /locus_tag="IXT21_000890"
     CDS             17516..18802
                     /locus_tag="IXT21_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4868316.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            18939..19808
                     /locus_tag="IXT21_000891"
     CDS             18939..19808
                     /locus_tag="IXT21_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4868317.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            19805..20692
                     /locus_tag="IXT21_000892"
     CDS             19805..20692
                     /locus_tag="IXT21_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4868318.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINCQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            20859..21542
                     /locus_tag="IXT21_000893"
     CDS             20859..21542
                     /locus_tag="IXT21_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ4868319.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            21539..22312
                     /locus_tag="IXT21_000894"
     CDS             21539..22312
                     /locus_tag="IXT21_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ4868320.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVTTGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            22309..22707
                     /locus_tag="IXT21_000895"
     CDS             22309..22707
                     /locus_tag="IXT21_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ4868321.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            22892..23563
                     /locus_tag="IXT21_000896"
                     /pseudo
     CDS             22892..23563
                     /locus_tag="IXT21_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23622..24062
                     /locus_tag="IXT21_000897"
     CDS             23622..24062
                     /locus_tag="IXT21_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868322.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            24074..24742
                     /locus_tag="IXT21_000898"
     CDS             24074..24742
                     /locus_tag="IXT21_000898"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ4868323.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            24806..25429
                     /locus_tag="IXT21_000899"
     CDS             24806..25429
                     /locus_tag="IXT21_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ4868324.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            25485..25871
                     /locus_tag="IXT21_000900"
     CDS             25485..25871
                     /locus_tag="IXT21_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ4868325.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            26083..27474
                     /locus_tag="IXT21_000901"
     CDS             26083..27474
                     /locus_tag="IXT21_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ4868326.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            27821..28468
                     /locus_tag="IXT21_000902"
     CDS             27821..28468
                     /locus_tag="IXT21_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ4868327.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            28861..31506
                     /locus_tag="IXT21_000903"
     CDS             28861..31506
                     /locus_tag="IXT21_000903"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ4868328.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            31816..33129
                     /locus_tag="IXT21_000904"
     CDS             31816..33129
                     /locus_tag="IXT21_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ4868329.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            33119..33772
                     /locus_tag="IXT21_000905"
     CDS             33119..33772
                     /locus_tag="IXT21_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4868330.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            33827..34519
                     /locus_tag="IXT21_000906"
     CDS             33827..34519
                     /locus_tag="IXT21_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ4868331.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            34669..34869
                     /locus_tag="IXT21_000907"
     CDS             34669..34869
                     /locus_tag="IXT21_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4868332.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(35123..36802)
                     /locus_tag="IXT21_000908"
     CDS             complement(35123..36802)
                     /locus_tag="IXT21_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ4868333.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(36804..37016)
                     /locus_tag="IXT21_000909"
     CDS             complement(36804..37016)
                     /locus_tag="IXT21_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ4868334.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            37409..39139
                     /gene="efrA"
                     /locus_tag="IXT21_000910"
     CDS             37409..39139
                     /gene="efrA"
                     /locus_tag="IXT21_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ4868335.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            39136..40896
                     /gene="efrB"
                     /locus_tag="IXT21_000911"
     CDS             39136..40896
                     /gene="efrB"
                     /locus_tag="IXT21_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ4868336.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            40991..41188
                     /locus_tag="IXT21_000912"
     CDS             40991..41188
                     /locus_tag="IXT21_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868337.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            41341..41826
                     /locus_tag="IXT21_000913"
     CDS             41341..41826
                     /locus_tag="IXT21_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ4868338.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(41930..42523)
                     /gene="clpP"
                     /locus_tag="IXT21_000914"
     CDS             complement(41930..42523)
                     /gene="clpP"
                     /locus_tag="IXT21_000914"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ4868339.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            42703..43230
                     /locus_tag="IXT21_000915"
     CDS             42703..43230
                     /locus_tag="IXT21_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ4868340.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            43321..44058
                     /locus_tag="IXT21_000916"
     CDS             43321..44058
                     /locus_tag="IXT21_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ4868341.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            44062..44979
                     /gene="miaA"
                     /locus_tag="IXT21_000917"
     CDS             44062..44979
                     /gene="miaA"
                     /locus_tag="IXT21_000917"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ4868342.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            44981..46210
                     /gene="hflX"
                     /locus_tag="IXT21_000918"
     CDS             44981..46210
                     /gene="hflX"
                     /locus_tag="IXT21_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ4868343.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        14806 a         8124 c        10030 g        13266 t
ORIGIN      
        1 acaacctctc tttcaatacc tttttgttct tgggataaaa ctttccctaa caatcaatcg
       61 tgcaggataa gtcaaaatga ttggttcgtc ccgtataccg tcaattcgct ccaatgccat
      121 tcgaacagct gcttgcccaa ttgatttcgc attgatttga acactggtca agccagggga
      181 gaggtttgcg attgtctcga tattatcaaa gcttgctaca acaatctctt ctccaatttg
      241 gatatttttt tgtttcaatg cccgatagac accaattgcc attggatcgc tcccgacaag
      301 aatggcagta ggaagagggg attcattttc taatagatta gtcgcaagta cctcaccttg
      361 tttttctgtc cagccaccta gatgataaga catatatttt tctaagtgat gatcgatcat
      421 aaaatgctga taggcacgca tcctcttttc atttatagaa atctgcttcc caccgaattc
      481 atccatatcg actttatatc ccccgatata ggctattctt ttatgaccat tttctaagaa
      541 caattccagt acggttttag tcatccgttc aagatctgca tacaccatat cgacctcttg
      601 ctggatgtca gggttatcca cgacgactat ttgcatgttt tgttttaaca aggcggtaat
      661 cgattctttc gtaacagtcc caacaacgat caccgcacct aactgatcta gatcttccca
      721 ttgtttagga ttatcagata gattatagat tcgattcatc cctaaatgca gacgtcgagc
      781 ttcttcttcc atccctactc gcaaatgttt gtaatagata tcctgttctt cgctcgcttt
      841 gccgatcgta gagatcacac caatcgaagg cattctcttt ttgatatatt tacgagtact
      901 gatgtcataa tccaacgcag cagctgcctc tactatccgt tgccgagtag tctgagccac
      961 atggatcgtc tgatcgttat tcaacacacg ggaaactgta gcaggagaga catttgcctt
     1021 tcgtgcgaca tctctaattc caaccatttt tacacactcc tcaaaaaaat aaaaaatttt
     1081 tattgacaaa attgtttagt aaacagtata caggaattat aaaaaaaagt aaacggtttc
     1141 cgttttgttg ggagtgaaat gatggatatt acgcatagaa aaacagataa aaatttcatc
     1201 ttatttgatg aaaagagccg agtcttccat ttacgaaata catttctttc ttatctgata
     1261 aagatagaag aaagcaatgt attggcgcat atttattttg gcaaaccagt caagcagtac
     1321 aaagacaata aaaattatcc ccgaagagat cgtggatttt caggaaatgt tcccttaaat
     1381 cctgaccggt ctctgtcgaa agatacacta ccgcaagaat actcttctca tggcagcatg
     1441 gactttcgaa cgcccgcatc gatcattcag cgcaagaatg gatcagactt actggatctg
     1501 agatatgaca gccattatat tacagatggg aagccagaga tcgaaggctt gcctcaaact
     1561 tatgtgatgg acaagtcaga ggcacaaacg ttggtgattt cacttaagga tagagaaaca
     1621 gcgatttatt tcgatttgtt ctataccata tttactgata gagcagtgat cacacgatct
     1681 gtaaagataa gaaatgaaac cggagaaaca atcaaactag aaaaagcggc tagtttccag
     1741 ctagactttg cccatactag gcgatttgat gaagtcattg ctttgccagg agcacatgtc
     1801 aatgaaagac agatttcacg gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt
     1861 cgaggaacga gcagtcacca tatgaataac tttattgctt tagttcatca tcatacgaca
     1921 gaaaatactg gagaagcgat cggacttcaa tttgtttatt ccggcaacca ttctttcgaa
     1981 cttgaaaaag atcagatcaa tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt
     2041 tcgtgggaat tgaatgcagg ccagtctttt caaacacctg aaatgatcct ttcttactca
     2101 agtcagggat tgaacaaaat gagtcagatc caccatgagt tgctacgtga aagaatcgca
     2161 agaggtcgtc atcaatttgc tgaacggccg attctggtca ataactggga agccacttac
     2221 tttgacttca atagtgaaaa aatcaaggcg atcattgatg aagcaaagga gctaggcatc
     2281 gaaatgttcg tattagatga tggctggttt ggaaaaagag atgcggacaa ttcttcatta
     2341 ggagactggt tcgagtatga aggcaagttg acaaatggat tacgtgaaat agcagattat
     2401 gctcatagca aaggattgaa atttggttta tggtttgaac ctgaaatgat ctcggttgat
     2461 tctgaactgt atcgaacaca tcctgatttt cttatgcagg agccaggtag aatgccttct
     2521 gcttccagat cacaacatgt actggatttt acaaggttag atgttcggca gacgattgag
     2581 aaacaaatga gaaagatttt agatacgatt cctcttgact acatcaaatg ggatatgaac
     2641 cgttcgcttt cagatgtcta ttcgattacc cttgacccac agagacaagg agaagtagcg
     2701 catcgttata tgctcgggct ttatgaacta cttgaacatt tgacaactga ttatccggaa
     2761 atcttatggg aaggctgttc tggtggaggt ggacggtttg atgccgggtt tatttattat
     2821 atgccgcagt catggacaag tgataacacg gatgcagtgg agcgtatgaa aatccaatat
     2881 ggaacaagcc ttgcttatcc gatttcgtcc atcacagctc atgtatcagc tgtaccaaat
     2941 catcagaccg acagatctac ttcgttgaaa acaagaggag aaacagctat gagcgctgtt
     3001 tttggttatg aactggattt gacaaagctg tctcctgaag aaaaaaagca agtaaaagac
     3061 caaattatat cctatcaaac gattcgtccg gtgatccagt atggccatta ttatcgtttg
     3121 gcgagtcctt ttgaagaaaa tattgccgca tggatgtttg tgtctcctaa acaagatgaa
     3181 gctattgtgt ttttagggag gatactagct tcagctcaac cagcgttcca tgaagtatat
     3241 ctgatggggt tagatgatga ggcactttat caggaacaga cctcgaagcg gatattttcg
     3301 ggggccgaat tgatgacagt tggactttac ttccccgatt ttcaaggtga tttccaaaca
     3361 gaactgcttc atttcaaaaa gttatgagag agaaggaaaa aagtatgaaa gcaaatataa
     3421 aatggttaga tgatccacaa gtttttagag tgaaccaatc aaatgcacat agcgatcatg
     3481 tttgttatgc aacaagggaa gaagtagaaa ccacatcctc ccttaaacag tctttaaatg
     3541 gatcatggcg tttttgttat tcgccaaatc cttcggtacg accaaaaaca ttttataaaa
     3601 aagattttga tgcctgttct tttgatgaaa tcactgttcc tagtcatatt gaattagcaa
     3661 attatgacaa gatccattac atcaatacga tgtatccgtg ggagggacat gtatttcgcc
     3721 gtcccccttt cacgacaagc cctgagaaag aggcggaagg gatcttttca gctgctaaag
     3781 acaatccggt tggttcgtat ttgacgactt ttcgattaga agaaggtcta aagggaaaac
     3841 aaatctcgat tcattttgct ggtgtagaac aagcaatgtt tatttggttg aatggtcatt
     3901 ttatcgggta tgccgaagat tcgttcactc cgtctgaatt tgatttaaca ccttatttgg
     3961 aagaagaaaa tattctagct gtggaggttt acaaaagaag tacagctgca ttcttggaag
     4021 atcaggactt tttccgattt tttgggatct tcagagatgt cttcttagtt gctcgaggac
     4081 aaaaccattt agaagatctt tggatcaaac ctcgcttaga tgacaaatgt caaaatggca
     4141 aacttgaagt agatttgtct tttgagcaag tagccaaaaa cagtcagctg aatgtagcga
     4201 tactagatcc tgatgggaag gaaatctatc aacagcaata tccgttaaaa gagcaaatga
     4261 cgattcgcat cgatggattt gaaaatgtgg cactgtgggg gcattttcag cccaatttat
     4321 ataagcttga attggagacg atagacgaag aaaaaagagt aacggagtat attgactatt
     4381 ccttcggttt tcgtaaaatc gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga
     4441 tgattatcaa cggggtaaat cgacatgagt ggcatccgaa aaaaggacgc gccatcacta
     4501 tagaagatat ggaaaaagat ttggctatca taaaaaatgc aaagatcaac ggcgtacgga
     4561 catctcatta ccccaaccag atcacttggt attatttatg cgaccaagca ggaatccatg
     4621 tgatggcgga aacgaacctc gaatcccatg gctcatggca aaaaatgggc gcatttgagc
     4681 cttcttacaa cgtaccaggt gcagtacctc agtggcaggc ggctgttctt gatcgagcaa
     4741 aaacgaattt cgaaacatta aaaaaccatc caagtattct tttttggtct cttggaaatg
     4801 aatcttatgc ggaagaaaac attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg
     4861 atcgacttgt ccattatgaa gggaattttc caaatccagc attcgaagat tcgatttcag
     4921 atgtaaaaag ctgcatgtat gcgccaccag aagagatcat tgactacctt gaaaataaag
     4981 cggaaaaacc gttcatctta tgtgaatata tgcatgacat ggggaattcg ttaggtggaa
     5041 tggattctta catgacgttg cttgatcgtt atgaaatgta ccaaggaggc tatatatggg
     5101 attttaaaga tcaggcgatc caagtgactg atccaatcac aggcagagaa gtgatgcgct
     5161 atggcggtga ctttgacgat aaaccaagtg actatgaatt ctcagggaat gggatcgttt
     5221 ttgcagatgg acaagaaaaa cccgccatgc aggaggtaag atattattat gaaaaataca
     5281 gtaaataaaa gtcatatgga tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta
     5341 ggtgtcaaat cggggaatac gcattatatt ttttcttata caagaggcgg actggaatcg
     5401 ctcaataaaa acggaaaaga atggctatac cgggaaacac ttccagtttt ttggcgagca
     5461 acgaccgata atgacagagg tagcggattt ggatttaaat ccggtatgtg gttaggagcc
     5521 ggattgtatc caaaagtcaa agaaatagaa gtactggtgg acaataaaaa gatcgatttg
     5581 ccgattgctc cagtaaataa tcaatattca agtaatgaat acgctgaaac ggttgaaatc
     5641 atttttacac tggaaatcaa cacagtacca aaaacccaag tgacaatttc ctaccaaatc
     5701 gctggagagg gtaaaatgct tgtcaatatg cattattatg gacaaaaagg attaccggaa
     5761 ttacccgcat tgggtttaag gttcattatg ccaacgaaag caaaattttt tgagtatgaa
     5821 ggcctatcag gggaaacata tccggatcgg atggctggtg gtgtcgaagg tatttataaa
     5881 gtggcaggac tccccgtttc cccatacctt gttccccaag aatgcggcat gcatatggaa
     5941 acgaaatggg tagaactaac aagaaatcaa acgttggata atacggataa agatcaagaa
     6001 gatttttcgt tgcgatttga acagcaggat acgccatttg ctttttccgc tttaccattc
     6061 aaagcagaag aactggaaca ggcaacacat atagaagaac taccgccaga aagacgaacg
     6121 gttcttacga tttttgcaaa agtaagagga gtcgggggta tcaatagctg gggagcggat
     6181 gttgagtcac cttaccgaat cagcagcgaa gaggaccatg aaatcggctt tattattcag
     6241 tagcaatctt tagattatta tttgaaaaac ggagtttaca agacaaagct gctttattct
     6301 atatgtcatg aacaagagag taaaaaagtt ctcagatact tttgttcatg actattttct
     6361 tcaataaaac caattgttat ttttctgaaa aacaaagtta actatcagaa aattaaaaaa
     6421 agtttcataa aaatgattga cagtcgattt ttttatgtct atactaggta caattcttac
     6481 atcagtttgg agcgattttt tatgaaatct agaaaactta caaaactgaa tacgtattac
     6541 tttagctatt ttagataggt ttgtattcat gacttcgtgg gtacaaacaa accaggtttg
     6601 tatctatgat ggctaaagat ttttaaacac gacttacagc cacgtagaga aattcatcta
     6661 tagtggctat tttatatttt tgcagcgctt tttaaggctc gttgaagatg ttcactgtag
     6721 aggcaatttc ctacagggac atcttttttt gttgattatc tgggtcataa gtaggaaaga
     6781 aggagcgaaa atgagaacaa caggacacgg tcatttagta ggaattaatg taaaaccacc
     6841 ttgaaaaagt agagaaaatc agccaagata gataagtaaa caaaagaaag cgggaaaaac
     6901 aatgatagta attatgaaaa aagaagcaac ggaagaacaa ataaaaaaag tcgtcgatca
     6961 tatcgaaaaa gaaggattca aagtctcaat cgatcaagga acagaacgag tagtgattgg
     7021 actaaaaggt gatacgcgaa gtttacaaga aggagcattt acacgctatg aaggagtaga
     7081 aaatgctgtg cgtattttaa acacatacaa attgacttct cgagaattcc atcctggcaa
     7141 tacagtcgtg gatgtagacg gagtaaaaat tggtgacggc agtttcgtga cgatggctgg
     7201 tccttgttct atcgaaggat tagaccaaat cagagaatgt gcgcgaatgg ccaaagccgg
     7261 gggcgcaaaa attctgcgag gtggtgcctt caaaccacgt acttctccat atgctttcca
     7321 aggtttagaa gaagaaggat tgaaatacat ccgtcaagcg gctgatgaat ttggaatgaa
     7381 agtgattact gaagtgatgg atgaagggca tatcgatatg gtggctgagt acagcgatat
     7441 cctacagatt ggcgcacgta atatgcaaaa ttttaaactg ctatctgctg tcggtaaaac
     7501 aggtaaacct gttggattga aacgtggaat ttccggtacg atcaatgaat ggcttaatgc
     7561 tgcagaatac attgcagtcg aagacaaatc tcctgtcatt tttatcgaac gtgggattcg
     7621 gacttacgaa acagcgacac gtaatacgtt tgatttaagt gctgtcccat tgatgaaaaa
     7681 attaactcat ttccctgtga ttgttgatcc aagtcatggt acaggaattt gggaacttgt
     7741 accaccaatg gctcgtgcgg gtgtggcaag cggagcagat ggcatgatcg tagagatcca
     7801 tcctgatcct gcaaatgctt ggtcagacgg tccccaatcg ttgaatgaaa agacttactc
     7861 acgcatgatg aaagaagttg cgatcatgaa agaagcaatg gaaaagattc agacactcga
     7921 ttaattccag ctttttaaaa gaaagaagtg aaacgaatgc ttactgtaca tgttgataac
     7981 caaaaatacg atattcatat tgaaaaaggc gcactagaac aggctggatc atggcttcaa
     8041 acgatttgga gacctcaaaa agtagctgtg atcactgata caaacgttgc tccactatat
     8101 gcagaaaaaa tcatgttcca attgagacaa gctaattttg aaccagtcct ttcaatcgta
     8161 ccggcgggtg agaccagtaa atctttagaa caagcagctg agttatacga ttttttagca
     8221 gataatgatt ttacccgcag cgatgggata cttgcacttg gtggaggaat ggtgggtgat
     8281 ctagcagcat tcgtggcctc gacgttcatg cgtgggatcc atttcgttca aattccgact
     8341 acgctacttg cacaagtaga ttctagtatt ggcgggaaga cagcagtcaa tacaaacaaa
     8401 gcgaaaaacc ttgtcggaac atttagccag ccagctggtg tattgattga tcctgaggtt
     8461 ctaaaaactt tgccggtacg tcgtttacga gaaggaatcg ctgaaatcat caaatcagca
     8521 gctatagcag atgcaacgct ttggaaacag ctgaacgagc tgaaagacga aaaagatctt
     8581 ctggaacatg ctgttccaat cattactgct acgttggaaa tcaaacgaaa agtagtggaa
     8641 gaagatgtct ttgaccaagg aaatcgactt attttaaatt ttggtcatac aattggccat
     8701 gcaattgaaa atacagcagg atatggggta gtcagtcatg gagaaggagt agctattgga
     8761 atggtagcca tcacttctca tgcagaaaaa atcggcttgt ctccaactgg atcgacagct
     8821 gctttagaaa ggatacttca aaaattccat ctgccgatta cgtacgatct aagtcatcca
     8881 gatgaattat tccacgcgat aacccatgat aagaaagcaa gaggaagcca attgaatatc
     8941 atcttgctag aaaagattgg gcaagcaaaa atcgttcctg ttcccacaga gacattcaaa
     9001 gattacttag gaggactgta agatgcgtta tatgactgcg ggagaatccc atggaccaga
     9061 attaacagcc attattgaag gactaccagc aggattacct ctgtcagaag aagaaatcaa
     9121 tcacgaactc gctcgcagac aagctggtta cggcagagga ggaaggatga agatcgaaaa
     9181 agatcgtgtt cggatcacgt ctggcatccg tcacggcaaa acaattggtt cacctgtcac
     9241 tttagtggta gaaaataaag attggaaaaa ttggacaact gtcatgtctg tagcagaagt
     9301 gcctgaaaaa gaccaatcac tgcggcgagt tgcacgtccg cgtccaggac acgcggattt
     9361 ggtaggcggt atgaaatacc atcatcgaga tcttcggaat gtgcttgaac ggtcctctgc
     9421 aagagagacg accatgcgtg tagcaatcgg cgcaattgcc aagaaaattc tggaagaact
     9481 tgacatcatc gtagcaggac acgtgatttg tcttggtgga atccaagcag aaatacctgg
     9541 aaatatctcg attcaagaaa tcaaagaaat ttctgaagcg tcagaagtac gagttgttga
     9601 tccagcagtt gagcaaagaa taaaagattt gatcgatcag acaaagaaaa atggagatac
     9661 gatcggagga attgtagaag tgctcgttgg aggcgttcca gctggattag gaagctacgt
     9721 acaatgggac acgaagctag atgccaaaat cgcacaagct gtggttagta tcaatgcctt
     9781 taaaggcgta gaatttgggg tcggattcac ttctggttca ttaccaggct cagaagtgat
     9841 ggatgagatc ctatgggaag aagaaagagg ctatttccga cgtaccaata atctaggcgg
     9901 cttcgaaggc ggaatgacca atggcgaacc acttgtcatt cgcggggtca tgaaacccat
     9961 tcctacattg tacaagccac ttcaaagtgt tgatatcgat acaaaagaac cttacaaagc
    10021 aagtgtagaa cgttcggata gtacagcagt tcctgcagct agcgtcgttg cggaaaatgt
    10081 tgtagccact gtggtagcga cagagctatt gtcaaaattc ccaagtgatt cgcttgttga
    10141 gttgaaagct gcagtgaagg agtaccgtga ctacttgaaa cattactaaa aagggggcaa
    10201 ataagatggc acaacatgtt tttatcgtgg ggcttggact gattggtgct tctatggcac
    10261 tttgtattcg tcagaccaat ccagaactga tagtagttgg ttgggatagt cagtcaaaaa
    10321 cccgtgagca agcagtagaa cagcagatcg ttgattacgt tgcaagtgat tttgaaacag
    10381 gtgcagaaca agcagacgtt atcttattag ccgttcccgt ccgtacaacc ttagcttatc
    10441 ttgatgtcct tgaaagaatc aacttgtctg atcacgtcat cattacggat gtcagcagta
    10501 cgaagcagca agtcgtgaca tccgctgagc agaaaaatct tcggtttgtc ggtggacatc
    10561 caatggctgg ttctcataaa tcaggtatac aggcagcaga tgctaaacta tttgaaaatg
    10621 cctattacat ctttacacct ttggaaaaga cgaaaaaaga tgttgaaaaa ctgcaagaac
    10681 tattttgcgg aaccaaagca aaatatgtaa ctcttacagc aagagaacat gaccgtataa
    10741 caggaatgct aagtcatttt ccacatattc tggcagctgg cttagtcaat caggcagaac
    10801 aattcaatca agagtatccg cgagccaaac aactggcagc aggaggcttt cgagatatca
    10861 ctaggatcgc ttcctcagat cctgtcatgt ggacagatat tttgttgagc aataagcaga
    10921 tattattgga acgattggca gattggcaac aagaaatgac acaaatagca gaatggatca
    10981 tgactgagaa ccaatcggaa atcttttctt ttttcaatcg ggcaaaagaa agtcgagacc
    11041 agttgcctgt ccataagcaa ggcgcgattc ctgccttttt tgatttattt atcgatgtgc
    11101 cagatgaacc tggcgttatt gcagaagtaa ctggactgat cggaaaagcg ggagtttctt
    11161 tgattaattt gaagatccaa gagaccaggg aagatatttt aggaatactg caaatcagtt
    11221 ttaaaaacca acaggatttg ttacaagcaa aaagaatcat tatgtctaat accagttatc
    11281 attgctggat caaataggag gctggataaa tgcaattatt acaacaaata catggattaa
    11341 gagggactgt taggatacca gcagataaat caatttccca tcgcagcatc atgtttggag
    11401 caattgctga aggaacgacg actatacaaa attttttgcg cgcagaagat tgtctgagta
    11461 ctttacatgc cttccaacaa ttaggtgtcg agatcgaaga agaggaagag gtgatcaaga
    11521 ttcatggtcg cggtagccac tcatttgtcc aaccaaccga atcaattgac atgggaaact
    11581 ccggtacgac catacgttta ttgatgggta ttttggctgg acagcctttt acgactactc
    11641 tgttcgggga tgcttcgttg tctaaacgtc caatggggcg agtgatggaa cctttacgcg
    11701 agatgggcgc tgacttgcaa ggagaagaaa atgaccagta tctaccaatc actgtgacag
    11761 gaacccgctc tttatcacct atccgataca atatgcctgt agccagtgcg caagtcaaat
    11821 ctgctttgct gtttgcggca ttacaagcag aaggcacatc cgtaatcgtt gaaaaagaac
    11881 gttcccgtaa ccatacggaa gaaatgattc gtcaatttgg cggaaggatc acagtggaag
    11941 ataaaacgat catagtgacc ggaccgcaaa aattaactgg tcaacagata actgttccag
    12001 gtgatatttc atcagctgct ttctttctag cagcaggact tcttgttccg gaaagccaac
    12061 tgttgttaaa aaatgtcggg gtcaatccaa caagaactgg tatcttagat gtgttggagg
    12121 agatgggcgc agcgattacc cagacgaatc acaatgaaca gaaccaatcg gctgatttaa
    12181 gtgtcaagac gagtcatttg aagaaagcaa cgattggtgg agaaatcatt ccgcgtctga
    12241 ttgatgaatt gcccattcta gctttagtgg ctactcaagc agaaggaatc acgatcatca
    12301 aagacgcaga agaattaaaa gtaaaagaaa ccaacagaat tgatgcggtg gcagaagaat
    12361 tacagaaaat gggagcaaag ataaaagcga cagcagacgg tctgatcatc catgggccga
    12421 cacctctgca tggcgcaaaa gtgtccagca gaggcgatca tcgaatcggt atgatgctgc
    12481 aagtagctgc tttgatagct gatggacctt gcgaactaga aggagctgaa gctgtttcaa
    12541 tctcttatcc ggcatttttt gacgatttag cagaacttgt gtcaggagga gaagctcatg
    12601 gctagcattt tgctgattgg tttcatgggc gctggaaaaa caacaatcgg taaagggtta
    12661 gctcaatgtt tacaaaaaga gtatgtagat ttagatacaa agattgagga acatatccaa
    12721 ttgtcgattg ccgaatattt tcactattac ggtgaaaaaa gctttcgaaa agtggagtca
    12781 gatattttaa gaaagctgtc aaatgaagac aaaattatcg cgactggtgg agggattgtc
    12841 caaagtgcag aaaatcggcg ttttttaaaa acacagccga ttgttcttta cttagaggca
    12901 gaagcggact gtctagttga tcgtatccaa caagatgaaa cgtctattcg acctttagct
    12961 cttggaaaaa caagagaaga aataaaagca ttattggcac aacgactttc ttggtatgaa
    13021 gaaagtgcga cacatcgaat caacacgacc aatcaaacac cagaaaagat catcgatacc
    13081 attatagaaa ggatcaaaag catatgaacg tcagctatct agggccagaa agttcattca
    13141 cttatcaggc ggcttgtcag ctatttccta cagaacaatt gactgcgtat gcttccatcc
    13201 ctgtttgttt gcgcgctttg tttcgcaaac aagtcgatct ggctgtcgtt cctgtagaga
    13261 attcattgga aggagctgtt catcacacaa tcgatcttct ttccaaacat ccagaagtcg
    13321 aagttaaaag tgaaatcgtt cttcctatca agcagcagct tttaggcaat ccagcaacaa
    13381 agattacaaa aatcctttca catccacaag cactagcaca atcacaacag tttttagaaa
    13441 cgcattatcc taatgttcct ttagtagcaa cagaatcaac aactgctgca gcaatgtatg
    13501 tagcagaaca tccgaaagaa gacgcagcag cgattgcttc attagaaaca gcacagcatg
    13561 ttggattgga gatcttagct gagaatatcc aagataatga gttgaaccag acacgttttt
    13621 ggatcgtcgg tgaccgaaag atgaccagcc agcagtcagc tccagtaaaa atgagtgtca
    13681 tcttgacact acctgctaat cggccaggga tgcttcataa aatgctggca gcttttggct
    13741 ggcgggaaat caacttaagt aagattgaat cccgaccatt gaaaactagc ttgggagaat
    13801 attttttcgt gatcgatttg ttgttggacc gtccaatgac attagtagag aatgcgctcc
    13861 aagaaatcaa gatgctcgga ggagaaagtc aaatattggg ctgttatcca gtgctgacag
    13921 tagaagagac gcgtagatga aagtcaagaa tactgattga tttatttaac ttaaaataaa
    13981 aaaatcagcg cttggctatt ctttattttt gatattctga ttgattttta ggaagaagcg
    14041 gtagacaaat cgttacagtc gttcctgtcc caatcactga ttggatcgtt aagccatatg
    14101 catcgccata cattaggcgt atccgctcat ctacatggct gatgccgatt cctgaaaaat
    14161 gatcattttt ttgtttggta ttttcagtat cgaagccatc gccgttatca atcacttcga
    14221 ataaaagaag ctgatttttt gttttgccgt acaccgtgat gaatccttgc gttttattgt
    14281 gaaaagcatg aagataggcg ttttcgataa ttggttgaag gatcatattc ggcatagata
    14341 atgactgcag ttcatcagga atcattattt tagccgagat tctttcgcca tatcgcatac
    14401 tcaaaagatc gatgtaactt tgtacgcttt tgatttcttc cttgactgga atcacttctt
    14461 cttttttccc aatcgtactt cttagtaaag taatgaaact gtcgattcct tgaactgctt
    14521 catctgtttt tttctgccaa atcaaaaatt tcaagctagt cagcgtatta taaataaaat
    14581 ggggctgtat ctgtgcttgc atagtttgga tttcaagcga acgtttcttc ttttcgtctt
    14641 ggatcgtctg ttcataatga tgctgagcat tacttagcaa ttgattgatc gtattcccta
    14701 gcaattggac ttcatggctt ccttgtacag gaacagtagc ttttaagcca tcttcctcta
    14761 gccgttttag cgaatcaatc aagcggtaga tcgattttgt gtgtcgatcc aaatacataa
    14821 ataccaatga gccagcaaaa gcaagtgtca cgatattata caaaataatc ggaaaaatca
    14881 cgcccatttg gttagataaa gaatgaagat cgacttgact gagtaatgta caatcctggc
    14941 ggtacagagg aagacgggtc aactgctgtc catttgaaat cgttgtttca cctactgtta
    15001 gttttttgaa aatcattgat cggctcccaa ttgtattttc ttcatttgat gcgatgatcg
    15061 tctgttcttc gttcatgaag gagatcttcg atatctcagg atttactaca gattgaaaaa
    15121 gattcgctag ttgcttggat gagatcgata aaacagcata tccgtaaacg tgattcaatg
    15181 tatttttcag ttttttgaca aagaagacgc tgggtgcttt cttatcgcga agagtcaatc
    15241 cgtggtgtgc aaaaagcatt tgtgtatcag cagcacgttc attcactctc tgagcaatcg
    15301 cagattgaaa aaaagaatcg gcagatacgg cagtgagcat gtcgtttccg acaaaagttc
    15361 ttccattttc tccaaagatc aatacatcgt aatcgatcat tggatggatt tttgaaagag
    15421 agtcattcat tttttggatt gttcttagtt gctgaagctg ttccatatgt tgggctggat
    15481 cattggaaaa atagcttttg acagctggat tagaatcaaa agcggctaat gtattctcga
    15541 ttgtatcatt caaactttca aattcagatt gaatcttttt tagtaccaca tcattcgatt
    15601 gattgaacag ctggatatag gcatcccttg atcgatgata aataaacaaa gaaatgacga
    15661 tggcttgcag aaaaagaccg atgaacaaaa tgaacagaag tttggcaaag agttcatgct
    15721 ctttgatttt ttttctcatt ttacaaaagt tcctttacgg tattgagaag gtgtcattcc
    15781 tgtctcttct ttaaaaactc tggagaaata acccagttcg gtatagccga ctttctcaca
    15841 gatttccgtc aaagtaagcc gtgtttccgt caaaaagagc ttggctttac tgatacggac
    15901 tttttttaga tattttgaaa agttgatacc aaatttttca gtaaagatgg ctgagagata
    15961 gctatatgaa aaatgatatt tttcggataa aagttgtaaa ttgatttctt tctcataatt
    16021 ttcctggacg aactcgtaga tcaaagtcaa gatatcgaaa ttgtcttttt ttgcatttgc
    16081 agaaaagtgg gtgagtttct caagtgtttc tttgacaaga atagaaaaat cttcgaaata
    16141 ctcagcatcg gctaataaag aaggaagggc gatcttgagc aaagaaagtt cttcattccc
    16201 aacatgctcg tcagccaaag cgttgagtaa tgcatagaaa cgattgattg cctcatgttt
    16261 caaataagct ggatcaatag gctgttccaa tatcgtcgtg aagaaattct caagttcttt
    16321 gacagctaaa gcgtagtctt tatgaacgat ttttgttaaa taactacttg cttgctgctc
    16381 gtggttggtt aataacggaa gaagttcatg ctgataagca aggttctttc ccttatgata
    16441 gaaacgttgt cctttggtgc aatttctgat ctcttggaat atttcattga tatcttggat
    16501 tgcaaaagaa tcagaaacaa caaaaaaatc acctggaaaa acttcttgac gaaacttttg
    16561 aacaaacttc ctaagtgtat ctttggaatc agtattgatt acatagccgg attcttccga
    16621 aaaggtagaa acaggataaa tagtaaacga atcgtcgcta tccatcaatg aatgggtgat
    16681 cgactgtttt tgtttagagc cttgttttga actgaatggc cgtttttcgg tatataacag
    16741 aaaaacaagg tgtgttcaaa aagaagaggg tcaagcaatg gacgagtttg tccagcaatc
    16801 tgctgtgaaa gattctcgca gatctgctca taacgattgg tgattgggct tttgttgggc
    16861 ttttttttga ttttttgtga aacggcagtt aaggaattta gaaaaaaatc aggactcaat
    16921 cgaggcttta aaagataatc agcgacgcct tctttaaaag aatctttaac catatcaaat
    16981 tcatcatagc tagagaggat aaggatctgt gtttcaggtt gttgctgttt taatagccgc
    17041 gataattcga ttccatttaa ttcaggcatc attacatctg tgaccacgat atcgattgca
    17101 tgtaccaaag aaaaatcaag cgctttcagc ggactggaaa aagtccctat taattgaaac
    17161 ccgtgctcag accaattgat caactgtttc attccatcta aaatcgtctg ttcatcatcg
    17221 ataagaatta cgcgtattgg cattttttta cccgccttaa gaatagtctt gtaatttaaa
    17281 aatacttatt tctaggagaa aatgcaagga tatttttatt ttaaagaaac ctcaccattt
    17341 cgatctttgg tctagatgct aagattattc cttttgtcat agttgatttg tattttttta
    17401 ttattttagg agaaattttt gaagtatctg aataataaag aaaccgtttt caaattttgg
    17461 tatgatgaat gtagcaaacg taaacaacta atggatggaa aaggagcgga taaaaatgaa
    17521 catcagaaag ttaattgctg gagtcgtttg tttagcggca gcatcaggtt tcttaacagg
    17581 atgcggaagt gctagtagta cagcagacaa agaagaaaaa gtcactgtct gggcgtggga
    17641 tgagacattc aatatcaaag cagtgaatga agcaaaaaaa gtttatgaga acgaagaaac
    17701 agagatagaa gttgttacga tgtctcagga tgatatcgtt cagaaactga acaccgcttt
    17761 agcgagcggg aatacagaag gattgcccaa tatcgtcttg attgaagatt ataggataca
    17821 aggctatttg acttcttatc cagatgcatt cgctaatttg tctgctattg taaaggaaga
    17881 tcattttgca ccgtataaat ttgcggtaaa caaagtagga gataaaatat acggtgttcc
    17941 tttcgattcg ggcgtaacag caaacttcta tcgtacggat ctgatggcag aagcaggtta
    18001 tacagaagag gatatggaga atctgaagtg ggacgagtat attcaagtag cgcgagatgt
    18061 caaagaaaag acgggaaaga aaatcgctga agtcaatcct tctgaccttg gtcgagtaag
    18121 gatgattatg caacaggcag gagaatggta tacatctgaa gatggggaga cagtcacgat
    18181 ccaagacaat gcatctttaa agtatggtct tgaattgttt gccactcttt tgaaagaaga
    18241 tctagtcgaa caaacctctg actggaatgc tggagtaact gcgatacaat caggagcagt
    18301 agcttctagc ccaactggcg cttggtattc ttcaacgatc caaggagcag aaaaccaatc
    18361 tggaaaatgg aagattgcac caatacctgc acttccagaa aatctgcaaa aagcgcaagc
    18421 ttctaacctt ggcggcgctg gctggtatgt gatcaaagga gtagctggag aagattcagc
    18481 gaaagatttc ctagaagcaa cgtttgcttc gaatgaagaa ctgatgggca ctcttgcgaa
    18541 agaaatcgga ctagtatcta cgatgcttag cgcgaatgaa caagaagctt atcaagaagc
    18601 ttctgaattt tacagtggtc aaaagatctt tgacgatttt tcaacatgga catctgaaat
    18661 accagaagtg aattacggac atgagaccta tgcaattgaa gcagtcgtag ctgaagctct
    18721 tcatcgaatc atcaatggag aagagacaga caaggtacta gcagatacac aaaaacaagt
    18781 agaagcacaa ttggctaatt gatcggtggt aaaaaagtga atttcttcgc tttttcatca
    18841 gacaaacgaa aagacaaaaa agatcacgtc tgccttacag atattgtctt tcttgtcttt
    18901 tcatgtagat aatcaatgat agaattggag ggaaaagaat gaagcagaaa gatttattca
    18961 aaaacgggca tgctttttta atcttgccag tcattttgat atcaatcatg gtttttattc
    19021 cgatggtttt ggctttgatc acgtcttttc aaagtggtcc ccctgtcaat atgacattta
    19081 acgggttagg taattatcag cggatgttga cagatgcaac ttttaaaaaa gcttttttta
    19141 atacattcct gtatttactt gtacaagtac cgatcatgtt gtttctagca ctttttattt
    19201 ccaacctttt aaatgataaa aagttgaaat ataaagggtt gtttcgaacc gctatctttc
    19261 ttccatgtat cacttcgatg gtcagttatt ctctgatcat gaaaagtcta ttttctcaat
    19321 ctggccttgt aaacaacttg ttgttgggca ttggtatgat tgacacaccg attggctggt
    19381 tgactcatcc aatttgggcg agagttctga tcattctttc gatcacttgg cgttggactg
    19441 gatacaatat gatttttttc ctttcaggca tgcaaaatat tgattcggag atttatgaag
    19501 cagcatctat tgacggtgct tctaaatggc agcagtttat caaagtgacg atcccaaatt
    19561 taaaaccaat cattttgttt acttccatta cttcgacgat cgggacattg cagctttttg
    19621 atgaagtaca aaatattact ggcgggggac cagcaaatgc aacgaccacc ctatctcaat
    19681 acatctataa tttgagttat aaattcacgc ctaattttgg atatgcggca gcagtttcct
    19741 ttgttattgt ttcctttatt gtgatattgt cgataatcca aacgaaagta ggaggagaaa
    19801 gtcgatgaaa aatatggtag aaaaagaaac aatcgaacgc cccaacgtca aaaaacaagc
    19861 attagaactt cgttcgacaa ttgggtcagt attaaagtat acagcattaa gcatcctatc
    19921 atttatttcc attttccctt ttgtctggat ggttttggga atgacgaata cacccgtgga
    19981 tatcacatcg ggaaaaataa agattggcag taacttgatc atcaattgtc aaaatttgtt
    20041 ttccaatgag ctgaatttta ctcgttccct ttggaattca gcgatgatcg ctttggtgac
    20101 cactgtgttt gcattgctga tttcgtcaat ggcaggatat ggatttgaga tttatcaaag
    20161 taaacgaaaa gacctaattt tcaatattct actgctatcg atgatggttc cttttgcggc
    20221 attgatgatc cctttatatc gaatgttttc acaattgaac ggaacggttt tgggaatcaa
    20281 cagtttcttg gttgtcatcc tgccatctgt tagcactgct tttttgatct tcttctttcg
    20341 acaaaatgtt aaatcttttc caaaagatct agtagaagcc gctcgattag acggtctaaa
    20401 agaactaagt atttttttta gagtgtatat gccgacggct aaaaatacct atgccgcggc
    20461 tgcaatcatc acattcatga gtagctggaa caattattta tggcccttag tagctttgca
    20521 atctcctgat aaacggacgg ttccgcttgt tttatctgct atgggtgctt cctatacacc
    20581 agactatgga atgatcatgg ctggattagt catcgctact ttaccaactg ccatcgtatt
    20641 ttttgttttg caaaagcaat tcgttcaagg aatgatcggc tctgtaaaat aaaaaaacac
    20701 aataagcgcg ttccttcaaa gtcatttttc caagtaaaaa gatacttttg ggacgtgtgt
    20761 tattttatgt tgagaaaaat taaatgataa cgttttaagt ttgtgctcga aaactctttc
    20821 attttaaggt aaacttaaag agtaaaggag gaggatacat gctaaaaaat tttaaagaag
    20881 tgattgctgc tatattgccg atgactgtcc tgattgtcat tttaacgttt gtttttgcac
    20941 cgttagaagg agaagacctc gcttcatttc tggttggtgc ggcaatcatg atgatcggaa
    21001 tgacgctctt tttgtttgga gcagattatt ctatgatgga agtcggagat ctagtaggga
    21061 aatatatgat caaaaagaaa agtttgaccg ttttggtcag tttgggattt gctatcggga
    21121 tcgtgatcac gattgctgaa ccttccgtgc aagttttggg acaacaagta tatgatatat
    21181 ctgacggaga gattggcaga gtgttgctga ttgggatcgt cagcgtcggc actggagtat
    21241 ttctagcctt tgcgctgctt cgggtcgtat tcaagctatc ctattatcaa ttgatggcta
    21301 taggctacat aggcgttttg atcgcctcat ttttcaccag ttccgaattt atgccgattg
    21361 cttttgattc ggggggagtg actactggtc cagtaactgt tccatttatc ttggcccttg
    21421 ccggagggat gactagtatg atcaaacaaa agaaaaatga aaatgacagt tttggaatgg
    21481 tcggtattgc ttcgttaggg ccgattcttg ccgtgatgat tttgggggtg atcttccaat
    21541 gagtttctgg cagcaagtga tagatggaat cgaaatcgtt accatggaag tcattttggc
    21601 agtggcacct atcgtaatcc tatttgtttt aatgaatatc attagttttc gtatgcgcaa
    21661 aaagcgtttc tttgacatta tgaaaggttt cttgataacg actattgggt tgatcctttt
    21721 cctgcacgga gtcaatatgg cttatgtacc ggtaggacag catttaggca gtgctattgc
    21781 gggattgcct tacaattata tactggttcc ccttggcttt ttaatggggt tcttagtagg
    21841 gtttgctgag ccggcgattc atgtaatgat caaacaggtt gaagaattga gcgaggggag
    21901 aatccgatcg aaaatcatgc tgatggtaat ttcaattggg atcggcgcag cagtaggatt
    21961 gtctatgtgg cgtttgctcg tcggattctc tctttattat ttcttgatcc caggatacgg
    22021 gctagcattt attttaggac gaaaagtgga caagatcttt ttagcaatgg cttttgataa
    22081 cggtggagta acaacgggcc cgatgtgttc gacatttatt ttatcaatga gcgtggcaat
    22141 cgctgcacaa attgaaggaa gagatccgat cattgacggg tttggtgtgg ttgcaatgat
    22201 tgcattgaca ccaatattat ccactttagt actaggatat atttataaac gaaaagatga
    22261 aaaagacaga atcgaaaaag aaagattgca aaaattgggg gaaagcaaat gaaagatctt
    22321 gtagcattga atcttcacat gattgtgacg atcgttgacg gaggaatggg cgaagatgtt
    22381 atcgactttt caaaagaagc aggtgcagca ggcggaacga tcttacatgg aagaggttct
    22441 ggtgtacatg attcagggaa atttttaggt ttggacattt cgccagaaaa agatattgtg
    22501 ttgacattag tacccgatag tttgacgaat aaagtaatgg atgcgattgg tcaaggcatc
    22561 aacatcgata ttccaggtaa tggtatttgt tttagtattg atgtagacaa agtcattggt
    22621 atcactaaga ttcatcaata tcgagaaatt atcggaatgc aagaactttt agatgaaaat
    22681 aacgatagag aaaagtctga atcatagaca agcgtttgaa gaggaaataa acacaaacaa
    22741 gaaggcggat gagcgttatt tccgcttttt tgtttgtatt tttctcgtga atacttatga
    22801 aataataaat ttttatgaaa ttctactcta agcatcagac tttcttttca aaatatgcta
    22861 ttttaaaaat gatgaaaaaa gggggagaaa aatgaatttt ttagttgttt taaaagatac
    22921 tctgattgag cgatctacat ttctatatgt tcaattgcag gaaaataaaa cattgctgca
    22981 tttttcacca gagttccatc tagaggggct aacattagga gaaatctatc aagcagaagg
    23041 gctgtcggct gttttgcact ttttttaggt agaattggaa ttaccagtaa aagattatat
    23101 cgagctttct cttgaatctt gggatcgagc agtggttgaa ctgtttccag aaggtttaat
    23161 gatcgataca aacgatggga agatccatct gactgcagca gaactgcaaa aacaaatgcg
    23221 ttatcaaata gatgacgaaa attctttttc tatttttaga cgtcagcaaa agattctaaa
    23281 aagcttttta aaagtgatca gtaaaaaacg gaatctgatc aaaacgacac agctgttgaa
    23341 aaaatatcca ggtctgcttc acacgtctat tcaatttcct caattgatta cgatgatcca
    23401 tcgttttata cgtatgcaac agctaccatc taagaagctg tttcttccat tgacggacac
    23461 atttcgagta gtcaacagag aagataaaaa gaaagtgatt gtgattgact ttaccagaaa
    23521 tcggaatgtc cttcaccaag ttgcaatgga aaaggccaat taaggctttt tctttttgga
    23581 tttttttgtc ataataaata ttagatgatg gaggagataa tatgatgcaa acatggttag
    23641 aaaggctaat acgatttcag cagaactatc acaaacttat gcagggaaga tatgcgaaat
    23701 ttgatcagtt gaatcgcggt ctattgatcg ctgcgcttgt ctttagtttg ctaagtcgtt
    23761 ggttgcccta tagaatcgga caattgttgt tcatcgtctt tttcggatgg atgatatttc
    23821 gtttcctatc aaaaaaaatc taccccagac taaatgagaa tcaacgttat ttgaaatatc
    23881 ttgatagagt caaagaaaaa tatcatcaga taaaaaacaa aaagcaagtc aaaatggatc
    23941 gaaaaagtta tacttttttt gagtgcccga attgtcatca aaagcagcgt gcacctaaag
    24001 gaaaaggacg aatacgtgtg acatgcaaaa catgtggtat aaaattcgaa acgaatgttt
    24061 aagggagaaa agaatgaaat tagtattatt acgtcatggc gaaagcgaag caaattttga
    24121 aaattactgg acaggatggc tagatgtcgc cttaacagaa aaaggaaaag aacaagcagc
    24181 agttgccggt caaaaaatgc ttgaagccgg acttcaattc gatgccgttt atacctctgt
    24241 tttgaaacgt gcgattctta ccggacaagt cgcactagaa gaaatgggtc agttatggct
    24301 tcctataata aaaacatggc gtctaaatga acggcattat ggggatcttg tgggtaaaaa
    24361 caaagataag atgaaagaaa tatacggaaa agatcaagta aaaacatggc gcagaggata
    24421 tacggaagtt ccgccgttaa ccgcagaaaa tagatttgac cgcagatatg atcacttgga
    24481 tccgcatttg atcccatacg gagaaagtct ggaaatgaca gtgaatcgga tcattccctt
    24541 atggcaagat catctagcac caaaactaaa agatcggcaa gatttactag ttgtgggtca
    24601 tggtaatagc atccgagcac tgacaaaata tttagaagat gttcctgaag atcaaatgga
    24661 tacgatcgat atcccaaatg cacaaccaat ccagtataca ttagcagatg atttgacaat
    24721 tctggacaaa aaaatacttt aaagaacacc tttatctaaa agtgagagat agaaaaaaga
    24781 agcaaaggaa tggaacaaaa ggaacatgaa aaattatata tgggactttg atggtacatt
    24841 gtttgatacg tatcccgcaa tggtagatgg tgcatggcaa gcactaaaag attttggtat
    24901 cagtatggac aaaaaagaaa tctacttcaa aatgaaaaag tattctacta gttatttgat
    24961 caatgaatca aacttgaatg ccagagagtt taatgaatta tttcaccgat atgaaaaaga
    25021 aagtacggaa gtttcccgtc catttccaga aacgaaacaa gtactggaaa tgctaaaaga
    25081 caacggcggt cgtcatttca tcctgaccca ccgtttgaca gaatcaacat ggggcctgtt
    25141 aaaagaacat cgattggcac atctgattga ggaagtagta gggatcgatc aggactttcc
    25201 tcgtaaacct gatccagcat ctttaaatta tttgatcgat acttttcatt tagaaagaac
    25261 agacacgatg atgatcggcg atcgtcgttt agatatcgaa gcagggaaaa atgcaggagt
    25321 cgctacttgt ctttacgaca tcgatcattt tctgggtgag atcccggcag attatgtggt
    25381 aggaaatctg aatgaaatcc ttaccttgca gagcatcatt aaaaagtagt atcccctttc
    25441 aaaggagata tgttataatt ttcataaagg agtgataaaa ttatatggaa atcagaagac
    25501 aaaaagaagt tgtagaagca tttcattatg atatgagatt gccgaaccaa gaagtagaaa
    25561 cagatttgaa agtaggcttc tcgcctttaa aagaaacgat cgaaaactat ccgaaagaac
    25621 atagcatcgt aggagcacgc ttagagttcc gtttagtgtt tgatacatat gttttatctg
    25681 gaagtgtgag tcagatcaat catatcctca atcggaaaat tgaaggtcaa aatgatgtca
    25741 cacaagaaga agtggacgag ctagtagcac cgttattcga tatcgtacgc cgaatgtctt
    25801 atgaggtgac agagatcgcc ttagatcagc ctggggtaaa attgaatttt caatcagaaa
    25861 aacaagaata gacagtaaaa acatacctgc gatagattaa agctatcaca ggtatgtttt
    25921 tttattacta attgtaagca aaaagaatgt catttcgata aaaatcgtct aaaatataaa
    25981 agtcactgaa aaaatttttt caggaggatg gttgaagaat cattctcggg aacctttact
    26041 tccttcgatg gacttgtcat taataatatg aggaggagaa aaatgacaac actcatcaca
    26101 gttttagtgg ttctagtttt tgcggcatta ttatatgtgt tctatcgtat gcaaaagcgc
    26161 catacaaaat tttcaacacg cgtctttaca gcattaggag ttggtatcgt ctttggcggt
    26221 ctgcttcaat tgatctttgg tgtagacgac aaagtcgtta ctcaatcaat ggaatggatc
    26281 gggattgtag gtcaaggata tgtatcattc ttgcaaatgc tagttatgcc tttagtgttt
    26341 atttcgatca ttggtgcatt cacaaaaatg aaagtttctg aaaagctagg gaaaattagt
    26401 gcgactgtat tgacttcttt acttggaaca acagctattg cagcattgat cggaattggt
    26461 tcaacaatgc tgttcggact ccaaggagct aaattcacac aaggagctgc agaaacagca
    26521 cgaatctctg aactggcaac acgtcaggaa acagttgaaa acttatcgat tccacagcag
    26581 attttatcct ttatccctaa aaacgttttt gctgatttag caggtagtcg tgcaacaagt
    26641 acgattggag tcgttatttt cgcggcattt gtcggaatcg cttatctagg cgtgaaaaga
    26701 aaagcgccaa aagaagcagc attcttttca gatttagtcg acagtctgta taaaatcatt
    26761 atgcggatcg tgacgcttgt tttgcgtttg acaccttatg gagtatttgc tttgatgaca
    26821 aacgttttgg caacaagtga ttttgcagct atcgtcaatt taggtaaatt tgtcgttgct
    26881 tcttacgtag cattgcttgc tgttttgatt gtccactcac tgattttgat tggtgcaaaa
    26941 gtcaatccaa tcaactattt caaaaaagcc tttccggtat tgagctttgc gtttacttca
    27001 cgttcaagtg ctggagcttt gccattgaat atcgagactc agacaaaagc tttaggagta
    27061 gatgatgcaa cagctaattt ctcagcaagt tttggtttgt ctattggaca aaacggctgt
    27121 gctggcgttt atccagctat gttggcaacg atcgtcgcac cagcagctgg agtgaatgtc
    27181 tttagtttgg aatttatttt gatgcttgtg gcaattgtaa caatcagttc ttttggtgta
    27241 gctggagtcg gtgggggcgc aacatttgct tcactgatcg ttttaggggc aatgaacttg
    27301 ccagtagcaa tcgttgggtt agttatctct gttgaaccat taatcgatat ggcacgtaca
    27361 gcagtaaacg tcagcggtag catggtagct ggtatcgtga caagtgcacg catcaaagat
    27421 ttagatcgcg atgtcatcaa tgatgattct aaagtgatcg atgtgaatgc atagtcaaaa
    27481 agaggttgtg atagaagtgg tcagctttaa gaaataagaa gaagtttcct aaaaatagtt
    27541 tctcaaattt ttgtgaaatt tcggcttatt tccgagaagc tacttctgaa acaccgtgaa
    27601 taagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagt
    27661 tcctcatatt ttacgtttgg ttgagcttat taccgcagat gcaagctttt gaacaccgtt
    27721 ttatttgtct cacacccttt tccttcctac ttgtaacctt gctcccgtgt tagaaacatg
    27781 ttacactaaa tatgtgaaat tattaacaag gagcgtcgtt atgcgagaaa aaacagatac
    27841 actgccaaaa gcaactgcga aaagattacc tttatattta cgctacttga agatgctgga
    27901 tgactcaggt ataagtagaa tcaaatcaaa tgagttcagc gaaatcaccc aaattccttc
    27961 tgcaacgatc cgcagagact tctcgcaatt aggcgagcta ggcagaagcg gatatggcta
    28021 tgatgtccct tttcttatcg atgtattcaa taatatcctt aatactaaag aagaaaaacg
    28081 aatcgcatta gtaggatatg gaaatcttgg caaagcattg aaacacaaca attttagaag
    28141 aaatgaaaat ctaaatatcg tgtgcgtctt tgataatgat ccagcactga tcaatcgagt
    28201 aattgatgga gaaatgatct atccgattga tcgtttcgcg gagatagcca aagcaaaaaa
    28261 tgtgacagta gcaatctcta cggtaccaag taaatattca caatctgcta ttgatgaaat
    28321 tgtcaaagga aatgtaaccg ctatacttaa ttttgcgcct gacagagtaa ctgtcccagc
    28381 ttatgtcaat gttcaataca ttgatttgac aaccgagttg cagacgctta tttactttga
    28441 tgagaattac tcagaagttt tttcttagga aaatttgagt aggcttattg atcttgcgag
    28501 aactcgtcgt aattctttca aaaatttttt agtactaaat gaagtggttt tttcacgacc
    28561 acttgttgac taaaaaaaaa aaccattgta gaatgaagca taacgctgaa caagaggagt
    28621 aactggcaca ttcattctta gagagagaac tatctgctga aaggttctca atgacaacca
    28681 gtgaaggtag cttgggagtt tctgtcttga atggagtaga gatagacgag tcgtgatcgt
    28741 taactcactt gagaggcaat aatgattgat tattgcaatt taggtggtac cgcgtaaatc
    28801 atacgtccta gaatacgaaa gtattttagg acgtttttta gtttaaagga gaaaaacagc
    28861 atgacggaag aaaataacct atcaacaaaa tataatccgc aagaagttga agctggacgt
    28921 tatcagcatt ggttagaaca agatttgttt aaaccgagcg gagataaaaa agcaaaacct
    28981 tattcaatcg taatcccgcc cccaaatgtt actggaaaac ttcatttagg gcatgcttgg
    29041 gatacgacct tgcaagatat gctgattcgt caaaagagaa tgcaaggata tgatacttta
    29101 tggctgcctg gtatggacca tgcaggaatc gcgacacaag caaaagtaga agcgaagctt
    29161 agagagcaag ggatttctcg ttatgatcta ggtcgtgaaa agttcatcga tcaagtgtgg
    29221 gaatggaaag aagagtatgc gagccacatc cgcgaacagt gggctaaaat gggcttatct
    29281 ttagattaca gtagggaacg ttttacatta gatgatggat tatctgaagc agtacgtaaa
    29341 gtttttgtta ccttatacga aaaaggtttg atttatcgtg gtgaatatat catcaactgg
    29401 gatcctcaag cacgaacagc tctatctgat atcgaagtta ttcataaaga tatcgaaggc
    29461 gccttttatc atatgagcta tgaactagca gatggatcag gcgtagtaga aattgcgaca
    29521 acccgtccag aaacgatgtt gggagatacg gcgattgctg ttcatccaga agacgagcgt
    29581 tatcaagcgc tcatcggcaa aaaagtgatt ctacctttag tcaataaaca aatcccaatc
    29641 attgcagatg aatatgtaga tatggaattc gggacaggtg tagtaaaaat cactccagca
    29701 catgatccaa acgactttga ggtaggtaat cgtcatgatc ttccacgcgt aaatgttatg
    29761 gatgatgatg gtacgatgaa tgaactagca ggaaaatatg ctggaatgga acgttttgct
    29821 gctcgtaaag caatcgtgca agatctgaaa gaaatcggtc gattgattaa aatcgatacg
    29881 atgatccaca gtgtgggtca ttccgagcgg acaggtgtcg ttgttgaacc tcggctgtct
    29941 actcaatggt ttgtaaaaat ggcgccgtta gcgaaaaaag ccattgataa ccaagaaaca
    30001 gatcaagcag tggaattctt ccctccaaga ttcaatcaaa cgtttttacg ttggatggaa
    30061 aatgtccatg attgggtaat ctcacgacaa ttatggtggg gacatcaaat tcctgcatgg
    30121 tatcacaaag aaacaggtga aatgtatgta gggatggaag caccagcgga tagtgaaaac
    30181 tgggtacaag atcctgatgt tttagacact tggtttagtt cagctttgtg gcctttctca
    30241 acaatgggct ggccagatac ggaaagcgaa gattataagc gatacttccc tactagcact
    30301 ttagtaaccg gttatgacat cattttcttc tgggtaagca gaatgatttt ccaaagcttg
    30361 gaatttactg gcgaacgtcc atttgagaat gtcttgattc atggattgat tcgtgacgaa
    30421 caagggcgga agatgagtaa atcattaggt aatggaatcg atccgatgga agtaatcgaa
    30481 aagtatggag cagatgctct ccgctggttc ttatcgaatg gttctgcacc aggacaagat
    30541 gtccgcttca gctatgaaaa aatggatgct tcttggaact ttatcaacaa aatctggaat
    30601 gcttcacgtt tcgttatcat gaatatcgaa ggaatgaccg tagaagagat cgatttatct
    30661 ggagaaaaat cagtggctga ccggtggatc cttactcgtt tgaatgaaac gatcgaacga
    30721 gtaactgagc tatttgatcg ttttgaattt ggtgaagcag gtcgacaact gtataacttt
    30781 atctgggatg atttttgtga ctggtacatc gaaatgagta aagaagtact ttatggagat
    30841 gatgctgttt caaaacaaac cacccgtagc atcttagtcc atacacttga tcagatccta
    30901 cgtttattgc atccaatcat gccattcgtt acagaagaaa tatgggaaca tattcctcat
    30961 cagggaaatt ctctagtagt ggcagaatat cctgttgtac gtccagagtt tgatgatgaa
    31021 acagcttcta aagggatgga agtattgaaa gaattgattc gagccgttcg aaatattcgt
    31081 tcagaagtga atacaccatt gtctaaacca attacgttgc taattaaaac aaacgatgct
    31141 aaaatcgatc aatttttagt tgaaaataca agctatatcg aacgcttctg taatcccgaa
    31201 gagttgacga tatcaagtga gatcactgcg ccagatctag cgatgtcagc tgttctgaca
    31261 ggtgcagaaa tcttcttgcc gttagcagga ttgatcaata ttgaagaaga aatcaaacga
    31321 ttagaaaaag aactggaaaa gtggacaagc gaagtaaaac gtgtccaagg aaaactttct
    31381 aatgaacgtt ttgtttctaa cgcgccggaa gaagtagtag aggcagagcg tgcaaaagaa
    31441 aaagattatt tagagaagca aacggctgtc aaagaacgaa tcgaaagttt gcgtactatt
    31501 caataagaat agaaaaagag agtgtgaaat cagctagcta ttactaaaag ctagctgatt
    31561 gaacatcgtt acaggaaaga gtttgagcct cactcttagc ttcgattcat aaggagatgt
    31621 gacataaatt ttgtcgcatc tcttttttag tctactctta ttatgtagga gaaggaggga
    31681 ggtggatcat gtatcaagag tcaataaagt agttgataaa atcgttcctc tctctgtttc
    31741 ttttctcttt gtcatacttt tatttttctt atgtgttcta tttgttataa taaaagagaa
    31801 atgggggaat tgttgatgac gattgaagaa gcaatagaat ggatccatag tcgattacca
    31861 tttggttcac gtcctggcct tgaccgagtc gaagctttat tagaagtggt agggcatcca
    31921 gagagaaaag taaaaacgat ccatatcgct ggaacaaacg gaaaaggttc tactgtagct
    31981 tatttgagaa gcctattaga agaggctggg ctgacagtag gtacatttac ttcaccttat
    32041 atcgaaacgt tcaatgaacg gatcgcaatc aatggcaaaa gcattccaga caatgatttg
    32101 atccagcttg taaaaaaata tcagccactg atcaaacaac ttgatcaatt tgaagcagta
    32161 gctggtatta ctgaattcga gacattaaca gcaatggcat ttgattattt tttgatgaag
    32221 aaagtggacg tagcgattat cgaagttggc ttaggcggct tattggattc taccaatgta
    32281 gctgtaccaa tgctgacggg aataacaacg attggacttg atcataccga aatacttgga
    32341 gatacaattg aagaaatcgc tgcacaaaaa gctggaatca tcaaacaagg cgttccagta
    32401 gtaacaggaa atattgcccc agaagctttt actgtcatcc aacaaacagc taaagcaaaa
    32461 aatgcaccgc tttatgtata cggggaaaat tataaaataa actatctgca tcctgatgag
    32521 atgtggggag aagtatttga attttttaat gaaataggaa agatccctca attaaccact
    32581 ccgcttttag ggcaacatca agctgaaaat gcaggagtag ccatcgaatt gtattttgtt
    32641 tactgtcaat tggaaaagct gcctttccga gaaaaagatg ttttgaaggg attgaagaaa
    32701 tctcgatggc caggaagaat ggagcgtttg agtaacgagc cattagttgt tttagatggt
    32761 gcccataata cgcatgcaat gaaccggctt gttgagaatt tgaaaaaaga attcaaagga
    32821 tatcaaatca acattttgtt ttccgcctta cgaactaaaa atgttagaga aatgctagaa
    32881 cgattgcagc aagtaccaaa tgtccatatt tatttgacga cctttgaaca tccaaaagcc
    32941 atcgaattaa gtgaagtatc agatttagca agtgagagag tatcaatcgt ttcattgtgg
    33001 caattcggat tagcagatat attggaaaag atgacaaatg aagacgtatt agttgtgacc
    33061 ggttcacttt atttcatttc agaagttcgc catttattaa tacaattagg aggagatcat
    33121 gaagtataat gcagtgattt ttgacatgga tggtttgctg ttcgacacag agttagtgta
    33181 ttacgaagcc acacaagtag tagcagacaa gatgggtttt ccttatgata aagacctgta
    33241 tttaaaattc ttaggtgttt cagatgaaga agtatgggcg aattatcata tgattttctc
    33301 ggaatttggc aaaacaagtg tacaaagatt catagatgat tcataccagg aaacgcttga
    33361 acgtttttcg caaggagcgg tacaaatgaa gcccggtgtg ttggaactcc tctctttttt
    33421 agaagaacat cagattccca aagttgtcgc ttccagtaac caacgagcag tcattgaact
    33481 tttattgaag aagaacaatc tgtttgaacg atttgagacg attgtttctg cagaaaatgt
    33541 tcaacgagca aaaccagacc cagaaatttt cttgttagct aatcagaagc taggtacagc
    33601 gaagaaagaa acattgattt tagaagattc acaaaatggt attttagcag cttctgcggc
    33661 tgagatcccg gtagtcatgg taccagatct gcttccacca tcccaagaat tgatcaaaaa
    33721 aacagctggg gttgtttctt ctttacatga aatccctgca ttgttaaaat aaaaatccat
    33781 ctcttttcgt atcttatttt agataagata gaaaggagat gtttttttgg gaaaacgatt
    33841 gatcaaagaa gtacctacta gctctttacc aagggaacgt atggaaattt atggcgcgga
    33901 agctttatcg gatcaggagc ttttggcaat cttactgcgt accggtcaac acccttatag
    33961 tgtgatgtcg attgctggaa atctgttaaa aacgtttgga ggactggcct ctttacgaca
    34021 ggctactttg catgagttag aagagattcg agggatagga cgcgtgaaag caatcgaaat
    34081 caaagccttg atcgaattag gtcgacgaat tcagacggat catgaaagag cggctccacc
    34141 ggttcgtacc agctacgaat tggcccagca attgattctg gaaatgaaag atcacaaaca
    34201 agaacatttg gtttgtattt atttggacac gaagaatcaa gtgatcttaa aaaaaacagt
    34261 gttcatcggt tcgctgaatc agtcagtcgc tcatcctaga gaaattttcc attatgctgt
    34321 acgttattgt gcagcaagga tcgttttagc gcataatcat ccttctggta atgttattcc
    34381 atcgcagcag gatatgaatt ttaccaaaag gatacaaaag tgtggagaaa tgatggggat
    34441 cactgtactg gaccacttga tcatcggacg caaacgatat tttagtttga gagaagaagg
    34501 aatgatggaa gagaagtaag ttgctttctt gcaaaaaaaa tgaagcaagt gtacaattat
    34561 attgtaattt tgtttgggaa tagaagttga aacatctgtt gcacctctat cgtacacgag
    34621 caatgttaca atttaaatta gtgcttttgg cacgaggagg atattattat ggaaaacgga
    34681 acagtaaaat ggttcaacgc tgaaaaagga tttggattta tctcacgtga agatggaagc
    34741 gacgtattcg ttcacttctc agctatccaa ggtgacggat tcaaaacatt agaagaaggt
    34801 caagcagtga ctttcgacgt tgaagaatca gaccgcggcc cacaagctgc aaacgttgta
    34861 aaagcttaat tttatctgta aatcaaattg atggtagaca tcaattttta cgagagaaca
    34921 aagcgagtaa caaaaatacc tgccacaaga gacaatttct cttgtggcag gtattttttt
    34981 gtatgaaaaa gtaaataaat ttaagaggct atgaaagagc caccttgtat caaggaataa
    35041 gaacaaacaa aaatggcttc tcatgttttt catttgtttt ctcttatatt caggacaagg
    35101 tgctgatttc ataacctctt atttattctt catctggtgt cattaccatt ggcaaaatca
    35161 ttggatgtct ttctgtacgt tcgaataaga aaggctgaag cgcgccagtc atcgcttctt
    35221 tcaatttata ttctgaacag tcaggtccat tcaaacattc tcttaaagcg tggaataaga
    35281 ttcgctgagc ttcattgatc atttctcctg attctcgcat gtagacaaat ccacgagaga
    35341 gaatatctgg accagccaag atttcttttt tcttgatatc cactgtcgcg acagctaata
    35401 caagaccttc ttctgaaagc aggtgacggt cacgtaaaac gacattacca atatcaccga
    35461 tcccgctacc atcgatatac acatctccag ccacaaaatg cccagcagga cgcgcagaat
    35521 cgctagttaa cgctaaaaca tcgccatttt ccatgatgaa gcagttttct gcaggaacac
    35581 ctacatcttg tgctagagaa gcatgtactt tcaacatgcg gaattctccg tggacaggaa
    35641 taaaatattt tggcttcatc aaacgaagca ttaatttttg ttcttcttgt ccaccgtgac
    35701 cggaagtatg gatgttattg atttttccgt gaatcacttc tgcaccagct tcagacagca
    35761 aattgatcaa atgattgaca cttgttgtat taccaggaat aggtgaactt gaaaagatca
    35821 ctgtatcgtc tggctggatt tggatttgac ggtgagtacc attcgcgatg cggctcaacg
    35881 cggccatcgc ttctccttga gaacctgtac ataggatcat cgtttcattg gcaggcaagc
    35941 gattgatttc gtgtgcatct acaaatgtgc cgtctggaac tttgatatat cctaaacgtt
    36001 gaccgttgac gatggcattt tccatgcttc gtccgaaaac agcgatttta cgaccagttg
    36061 ccacagcggc atctgctgct tgctgcaaac ggaagatgtt agaagcaaag ctggcaaaaa
    36121 tgatacgtcc ttcgattttt gaaaagatct tcaaaataga atgcccaatc gttttttctg
    36181 atttagtaaa tgttggaatc tcagcatttg tactatcaga taaaaggcac aagactcctt
    36241 cttccccgat acgagccatt ttatgcaagt ttgctggttc gccaacagga gtgaaatcga
    36301 atttgaagtc tccagtcgct acgatatttc cagaaggtgt tttcaccacg atccctaatg
    36361 catctgggat actatgggtc gttctgaaaa agctgacaga agtctttcta aaacggatga
    36421 tcgtatcttc attgatctca tggagctctg catctcgaag caagccatgc tcatctaatt
    36481 tgtttgtgat caacgctaat gcgagaggac cagcatagat aggaatattt gcttgtttca
    36541 gcaagtaggg tattccgccg atgtggtctt cgtgcccgtg agtaatcacc aaggctttta
    36601 ctttgtgtaa gttttgtatg atgtagctgt agtcgggaat gacatagtca attccaagta
    36661 aatcgtcttc cgggaatttg atcccggcat caatcaaaat gatttcatct tggaattgta
    36721 ccccatacgt atttttcccg atttcaccta atccaccaat agcaaagacg cctgtctcgt
    36781 tatttttgat ggaaagtttc atattaaaac tccactaatg agaaatcagc attttctttt
    36841 tcgtactcta gatgacttcc ttctagaagt tgaacgaatt caatattgta tggggtattt
    36901 tcttccacac gttggcgagc gtctacatcg ctttttgcct caatgtaaag agattttgtt
    36961 tcttcccgtt tagggttgcg tacttttgtt tcttgataat aaactttata gatcattgtt
    37021 ttgtctcctt tatctgcgta aagcagtgat ttagctagtt gattttttta gacaacacaa
    37081 aacgagggga caagccacct ggtttttcat atgctttgac tagaatactg gattcgagtc
    37141 aaagagacaa gcctcgtttt tgcttatctg ttcggatatt ttttcaaccg taccttctgt
    37201 ataaaaaata tacaacaatt aattgttatt ctagcataaa acagcaaata agtatagaaa
    37261 tcaactagat tgaaagaaaa aatcaatgtt tatcatcccg ctataatcat aatgactttt
    37321 ttatgagaga agtgttctat taataggctc gttgtgctat catttttagt aatgaaaatg
    37381 taacaaaaga ttaaaaggag atgagaaagt gaaactaatg tggcattata ccatgcgtta
    37441 taagaaattc ttgtttttca atttcatatg cgtatttggt tttattttga ttgaattagg
    37501 acttcctacg attttagcac gtatgatcga tgtaggaatc cgcaatgatg attttgatta
    37561 tgtgaagcaa caagggatat ttatgatcgt tattactgta atcggtatta ttttgaatat
    37621 cttgttaggt tattttggtt cgaggattac gactaatatc gtagcagata ttcgagatga
    37681 tttgttcgaa aagatccaaa gttattcgca tcaggaatac gaaaatctag gcgtatcttc
    37741 attgatcact cgtacaacaa acgatgctta tcagatcatg ttgttcctgc aaaatatttt
    37801 acgtatcggt tttatggcac caatgatgtt cattgtcagt ttgtacatgg tcatgcgtac
    37861 aagtgtgaca ctcagtttgt atgtagtagg tgcattacct ttactattat tggcagtcgt
    37921 agcgattgct aaggtgtctg aaccgttatc taaaaagcaa caaaaaaact tagataaaat
    37981 caacagtatc ttgcgggaaa atctttcagg attgcgtgtg attcgtgcat tcgtcaatga
    38041 aaagtttgaa gaaaaacgtt ttgctgtagt caatgagaat tatgcttcta gttcaaaaag
    38101 cttattccgt ttgatggctg ttgcacaacc gggtttttat ttcttattta atatcgtaat
    38161 ggtattgatc atctggagcg gggcagtgca aatcgatgca ggcgagcttc gtgtaggaga
    38221 tttgattgcg tttatcgagt atatattcca tgccttgttc tcattcatgt tatttgctag
    38281 tgtgttcatg atgtatccaa gagcagctgt ttcagctgga cgtatccaag aagccctaga
    38341 tgccaaacct tcgattgtag aagatccaaa tggtatatct gatacaaaaa ctaaagggta
    38401 tatcgaattt caaaatgtaa catttgctta tccaggccat tcgcaagagc cagtgatacg
    38461 aaatgtcagt tttacagcct ctccaggaga gaccgttgct tttattggaa gtaccggtag
    38521 cggaaaatct acgttgatcc aattgatccc tcgcttttat gatgtgagtg aaggaaagat
    38581 cttgatcgat ggagtagacg tgagagatta tcggctaagc aagttgcgag ataagattgg
    38641 gtttattccg caaaaagcgc tgctatttac tggaacgatt gcagataatt tgcgatacgg
    38701 gaaagaagat gctacgcaag aagaaatgga acgtgcagct gagattgctc aagcttcaga
    38761 attcatttct cgaaaaccag atggatttaa tgaacattta tcagaaggtg gttccaattt
    38821 ttcaggtgga caaaaacaac gattggcaat agccagagcc atcatacgcc gtccggaaat
    38881 ctatattttt gatgacagct tttctgcatt agattatcaa acggatgcga agttacgtgc
    38941 tcgtctgaaa aaagaaacga cagaatctac tgtattgatc gtagcacaac gtgtgggaac
    39001 gatcatgcat gcagataaga tcatcgtatt aaatgaagga gaagtcgtcg gtatggggac
    39061 gcatcgggag ttgttggaaa aatgcccgat ttattatgat attgccgctt ctcaattatc
    39121 aaaggaggaa ttagcatgaa aaaaaccttt tcttccttaa aacgtttagc tcgatatatc
    39181 agaccgtatc gtgagacttt tcttttagtg atcctattta cagttctgac agtggctttc
    39241 aacgtggctt tgccatatgt tacaggactt cctactacag aaatcagccg taatattgcc
    39301 aatggcgaat caatcaattt ttcttatata ttcaaatgtc tcatttggat cacagttgtt
    39361 ggaatagggt attgtatttc gcaattactt tctggtgttt tgatgacaaa tgtcgttcaa
    39421 agtgccatgc atgatttgag aagagacata gaagaaaaaa tcaatcgtct tccggtttca
    39481 tatttcgata aaaaccagca aggaaatatt ttgtctcgtg tgacgaatga tgtagatgca
    39541 gtcagcggtg ctttacaaca agcttttatt ggtatcgtaa atgcgatatt gggaattacg
    39601 ttggctgcag caatgatgtt ttatatccag ccgatgatgg cgctgatctc tatgatcatg
    39661 attccaacat cgatctggat ctcaaaacga gtgatcaatg cttcgcagaa atattttcaa
    39721 agcatgcaga attctcttgg tgagctaaac ggctacgtcc aagaaaatat gacaggtttt
    39781 agtgtattga aagtatatgg acgcgaaaaa gaaacattgg aaggattcaa aaaagttaat
    39841 cacagcttga aatactttgg gtttcgtgca gcattcattt ctggcttgat gatgcctcta
    39901 gttcaattaa ctgcctatgc tacttatatt ggtatggctg ttctaggaag cttttatgtt
    39961 attactgggg cgatcgttgt cggtcaacta caagcattca tccaatatat ttggcaaatc
    40021 agtcagccaa tggggaacgt gacacaattg tcttcagttt tgcaaagtgc atcagcggca
    40081 acgaaacgtg tattcgagat tttagatgag ccagaagaaa aagaaaacaa agtagatgct
    40141 tcactgccag aaacgatttt aggagatgtg acattcgatc atgtcgattt tgcatatgat
    40201 ccgaagaaac cattaatcca agatttgaat tttgaagtga aagctggtca aaccgtagct
    40261 gtggtcggtc cgaccggtgc cggcaagacg acattgatca atctattgat gcgtttttat
    40321 gacgtagaca aaggagcaat aaggattgat ggaatcgaca ccaaatcaat gagtcgcagt
    40381 gatgtgcgtt cgctatttgg tatggtcttg caagatgcat ggctttatca aggaacgatt
    40441 gcagataata tccgcttcgg aaagcttgat gcgacagatt atgaagtagt tgatgctgcg
    40501 aaaacagcaa acgtagatca ttttatccga acgatgccag atggatatga tatggccatc
    40561 aattctgagg gagacaatgt gtctttagga caaaaacagt tgctgacgat tgcccgagca
    40621 gtggtctccg atcctaaaat cttgatttta gatgaagcaa caagttcagt agacacccgt
    40681 ttagaagcgt tgattcaaaa agcaatggat cgcgtgatgg agggacggac aagtttcgtc
    40741 atcgctcacc gtttatcgac aatccgtgat gcagatctga ttctagtgat ggatcaaggt
    40801 cagatcattg aaaaaggaac gcatgagagt ttattggcac aaggtggatt ttatgaaaaa
    40861 ctgtataaca gccaatttgc tgaggaagca gaataagtca tataagaata aaacagttta
    40921 aaacttattt tttgttctct attcttgata atggtaaagt aaagttatca agaaaagtga
    40981 ggacacggag atggaacagt ttttaaatga aaaaattact gaagccaaaa gagaagttga
    41041 tcgtttaacg cacgtctgtc agacgaagcc ttttgattca gctgattttt ttgaaaatga
    41101 gtttcaaatc gaacgcttgg cagcacaaat agaagtgtac gaattaattt taaaaaaagt
    41161 aatagaagaa aatatagatg tacaataaaa acaggtaaaa cgtcagttac cagatactgt
    41221 aaatagacat ctggcctgag caggttttac ctgttttttt tcttatgaaa taaatatatg
    41281 ctatgatacc tttattcaat aggattagtt tctacaaaaa atgaaaggag aaaaataaat
    41341 atggaacggt ttatagcatc aaatgccaca gtgattggtg atgtgacatt gtcagaagat
    41401 gtgacgatct ggtatcaagc tgttttacgc ggggacagta attggataaa aataggacaa
    41461 agaacgaata tccaagatgg tacaatcatc catgtcgatc atgatgcacc tgtagatatt
    41521 gcggagaatg tgacggtagg acatcagtgc atgctacatg gctgtacaat cgaaaaaggg
    41581 gcgctgatcg ggatggggac cacgatattg aatcatgcgg ttattggtga gaatagtttg
    41641 attggtgctg gatcattagt aacagaagga aaagttattc ctcctaatgt cttggctttt
    41701 ggtcgcccag ctaaagtgat ccgtcctttg acaaaagaag aaatacaaaa aaacaaagaa
    41761 aacatccagc attatgtcga gataggtcgg atgcatgcgc aaggaaaatt caaggagcaa
    41821 agataggcag tagcatccat aaaataaaaa aggatggtca gtttgagttg accatccttt
    41881 tttatttggt ttagataaga gagcttaaaa aagcttcctt tgaaacagat taatttaatg
    41941 aagaactgtt ttccatgact tcatcgatca atccatatgc ttttgcttgt tcagctgtca
    42001 tatagttgtc acgatctgta tctttttcaa tcacttcaag cggttgtcca gtgcgttcag
    42061 ctaaaatttt gtttaagcgt tcgcgtgttt gcaaaatatg gcgtgccgca atttcgattt
    42121 cagttgcttg cccttgagcg ccgccaagag gttgatggat catgatctca gcatttggta
    42181 aagcaaatcg tttacctttt gtaccagctg tcagcaagaa gcttcccatt gaagcagcca
    42241 tacccataac aattgtttgg acatctgctt tgacaaagtt cattgtgtcg taaatagcca
    42301 tacctgctgt aactgaacca ccaggagaat tgatataaag atagatatct ttttcagaat
    42361 cttgagcgtc taagaaaagc aattgggcga tgatcgaatt agcaagatca tctgttactt
    42421 ggccgcttaa catgatgata cggtctttca ataaacgtga gtagatatcg taagctcttt
    42481 cacctcgtga agattgttca atgactgtag gaattaaatt cataatagag ttcctccttg
    42541 atttcgttta gaaatagtgt accataattt agtttctaat acctaaaatg tagtatacat
    42601 ttttggtcaa aaaaggtcaa ataaaaaact taggcaaatt agtcgcataa acccgcttta
    42661 tggtatgata agcagagaaa caaaacaagg aggaacaaca gattgatagt tggtattact
    42721 ggccttatcg gagtgctgac aggtgctgta gttgtctata tcggcatgat actgcaaatg
    42781 cgtcacttgg aaaataaaga acagcgtaga cgtgaattgg aattgaaagt caaagaaatc
    42841 gagacgctga atcaattaaa taaaaagatc aacgagatct tgcaaaaacg gacaacgctg
    42901 atggaagaat atgtcagctt tgatgcattt gatgattgtt atatcacgat tgacgatttt
    42961 gcgtatctcc aatcattttc agcgcaaaat agtttttatc tgccaaatta tattttagac
    43021 gagctattta agaatatctc ccatcgaaaa gtgattttat ctccagatga aacaatgaag
    43081 attggtggat atgcttataa aggcggacgc gtgattttag agaattttac ggatagtctg
    43141 actgaaatga tcaatgaaaa aaaagctcag ttgaagacta tgacgcgcca gccgattggt
    43201 ttcttctcga aagatgatgt ttggagctga tattttgaag cattgccgtt gcacatgaga
    43261 gcgtggtaat gctttctttt tttcttaatg atacaataga actgaacgga gtgaagaaag
    43321 atgttacaaa tctatgccca tcgcggaagc agcggcaccc acccagaaaa tacgcttcct
    43381 gcatttgcag aagctgtacg agtaggtgca gatggtatcg aactagatgt ccatctaagc
    43441 aaagatggtt atttgatcgt tatgcacgac gaagaagtgg accggacgac taacggtaaa
    43501 gggctgattc gagaaaaaac attggaagaa ctcaaaaaat tgaatgcagg gggcaaattc
    43561 agcaaagaat ttccagcagc taaagttcct gaattgagag aagtgttagg actgctcctc
    43621 caaaaaaatt accgagggat gctaatgatt gaaattaaaa ctgatcagta tgaatatccc
    43681 ggtatcgaag aaaaattagc cgaaacatta aaaagtagag attggccttt tcaacatgca
    43741 tattgcagct ttcatttgga ttctttggaa aaaatcgcat tactggaacc ggagatacag
    43801 ctggacttat tgatgagtac ttctgataaa aagatccaac tagcagcaga aaccccttat
    43861 atagaaggag tacatccaaa aaatacttgg ttgttcgaac atccagtcca ggcagctgat
    43921 tttccaaaag caattcgtgc atggacgatt aacgatgaag aagaaatggc caaatgtcaa
    43981 gaactcaaaa tagatggaat aataactgat tttccggaga aagctcttcg gtggaaacaa
    44041 tcaacgaaaa gtaggtaaaa tatggaaaaa gtattagtga tcgtcggtcc gacagcagtc
    44101 ggtaaaacag cgctaagtgt ggaactcgca caactatttg gaggagagat catcagtggt
    44161 gattcattgc aaatctataa aaatctggat atcggtacag caaaaatcaa agaagaagag
    44221 atgaataatg ttccgcatca tttgattgat gtgatcgaac ctactcaacg ttactcagcc
    44281 gctgattttc agcattcagg tcgacaattg attaccgaga ttgcgaaccg agggcaccta
    44341 cctatcatag ctggtggtac gggtctctat atccaatccc ttctatatga ttatcagtta
    44401 ggagcgacag aagaagcgaa tcctgagatt agaaaaaagt atgaaacgtt agctgaaaca
    44461 attggttccc aagcattatg gaatcagttg aaagaaaaag acccggcagc tgctgagaaa
    44521 atccactgga acaatcaacg caaagtgata agagctctgg aagttttaga gacgacagga
    44581 catagtatca cggcacctaa agaagagcca aagcaattgt atgattacct catgattgga
    44641 ttagatacga agcgtagtat tctttatgaa cgaatcaatc aaagagtcga tttgatgcta
    44701 gcagaaggtt tagtagcaga agcgcgtaag gtgtatgagc ttggagaagt ccaggccagt
    44761 caaggtattg gatataaaga atttttccct tattttgaag ggaaagaaac attggaagaa
    44821 gcaacagaac aagtcaaact acattcgaga agatacgcga aacgtcagtt gacgtggttt
    44881 cgtaaccgtt tggatgcaca gtggtttgat cttataagtg agccaaatca aaaaagtcag
    44941 attgagcaaa caatcaaaaa atggctggag gcggaataaa atggaagaaa aagtgatcat
    45001 cgtaggggta gaaaccgaag caaaccagcg ttacttttca gagtcaatgg aagagctagt
    45061 tcagctgacg aataccgcat caggagaagt cgtattcaca attacacaaa agagaccaca
    45121 agtggatcgg caaacgatta tcggtaaagg gaaattacaa gaattagttc aacaagcgga
    45181 tgcgcatgaa gcggatttga tcatctttaa tcatgagcta actccgagac agagccaact
    45241 gattaccgat gctgttggtc ttccggtaat tgatcgcgtg cagcttatct tagatatttt
    45301 tgcgatgcgt gctcgttcga aagaagggaa gctgcaggtt gaacttgctc agttagaata
    45361 cctgctgcct cgtttagtag ggcaagggaa aacattatct cgtcttggcg gtgggatcgg
    45421 aacaagaggg ccaggtgaaa caaagttaga aacagaccgc cgacatatac ggaataaaat
    45481 ttttgctgtt aagaaagaat tgaaagaagt agaagctcat cgagagcgca atcgccaaaa
    45541 aagaaaaaat agcgagattt ttcagattgg acttattggc tacacgaatg ccgggaagtc
    45601 aactatcttg aatctattga cacaagcgga tacttattcg aaagatcaat tgtttgcaac
    45661 gttagatcca ttgactaaga gatggcgttt tgctgaagga tttgagatta ctgtaacgga
    45721 taccgtcggg ttcatccaag acctgcctac gcaactgatc gatgctttcc attctacatt
    45781 agaagaaagt caaaatatgg atcttttgct acatgtagtc gatgctagtt ctccagatcg
    45841 tattttacag gagcagactg tcttaaaatt gatggatgaa ctgaatatga aagaaatgcc
    45901 gatactgact gtttacaata aggcagacca gatcgaccca gcgatgttca cgcctacgct
    45961 attcccaaat gtattgattt ctactcagac gaaagaaggt aagatggcgt tgatcgaagc
    46021 aatcaaacgt cagctgatgg aactaatgac cccttataca ctttctgtgc caagcaacga
    46081 aggacagaaa ttaagcgagt tgagaagaca aacaatggtt ttggacgaag cgtacatgga
    46141 agaaacaaac gaatatgaag ttaaagggtt tgcaaaaacc aattcaaaat ggttaagaaa
    46201 aaaccaataa aattttattt ttttaa
//
LOCUS       DACGLH010000015        41411 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.016, whole genome shotgun sequence.
ACCESSION   DACGLH010000015 DACGLH010000000
VERSION     DACGLH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.016"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(204..638)
                     /locus_tag="IXT21_000919"
     CDS             complement(204..638)
                     /locus_tag="IXT21_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868344.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            786..1927
                     /locus_tag="IXT21_000920"
                     /pseudo
     CDS             786..1927
                     /locus_tag="IXT21_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2053..3132)
                     /locus_tag="IXT21_000921"
     CDS             complement(2053..3132)
                     /locus_tag="IXT21_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4868345.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3235..3675)
                     /locus_tag="IXT21_000922"
     CDS             complement(3235..3675)
                     /locus_tag="IXT21_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4868346.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3732..4142)
                     /locus_tag="IXT21_000923"
     CDS             complement(3732..4142)
                     /locus_tag="IXT21_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ4868347.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4322..4756)
                     /locus_tag="IXT21_000924"
     CDS             complement(4322..4756)
                     /locus_tag="IXT21_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ4868348.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4856..5704)
                     /locus_tag="IXT21_000925"
     CDS             complement(4856..5704)
                     /locus_tag="IXT21_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4868349.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6093..7859
                     /locus_tag="IXT21_000926"
     CDS             6093..7859
                     /locus_tag="IXT21_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ4868350.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7961..9220
                     /locus_tag="IXT21_000927"
     CDS             7961..9220
                     /locus_tag="IXT21_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4868351.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9367..10668
                     /locus_tag="IXT21_000928"
     CDS             9367..10668
                     /locus_tag="IXT21_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4868352.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10671..11522
                     /locus_tag="IXT21_000929"
     CDS             10671..11522
                     /locus_tag="IXT21_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4868353.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11535..12350
                     /locus_tag="IXT21_000930"
     CDS             11535..12350
                     /locus_tag="IXT21_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ4868354.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12501..13667
                     /locus_tag="IXT21_000931"
     CDS             12501..13667
                     /locus_tag="IXT21_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4868355.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13897..14544
                     /locus_tag="IXT21_000932"
     CDS             13897..14544
                     /locus_tag="IXT21_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ4868356.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14628..15572
                     /locus_tag="IXT21_000933"
     CDS             14628..15572
                     /locus_tag="IXT21_000933"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ4868357.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15707..17158
                     /locus_tag="IXT21_000934"
     CDS             15707..17158
                     /locus_tag="IXT21_000934"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ4868358.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17179..18015
                     /locus_tag="IXT21_000935"
     CDS             17179..18015
                     /locus_tag="IXT21_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ4868359.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18015..18674
                     /locus_tag="IXT21_000936"
     CDS             18015..18674
                     /locus_tag="IXT21_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4868360.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGFSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18800..19489
                     /locus_tag="IXT21_000937"
     CDS             18800..19489
                     /locus_tag="IXT21_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868361.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19495..20451
                     /locus_tag="IXT21_000938"
     CDS             19495..20451
                     /locus_tag="IXT21_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ4868362.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20495..21007
                     /locus_tag="IXT21_000939"
     CDS             20495..21007
                     /locus_tag="IXT21_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4868363.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21263..21811)
                     /locus_tag="IXT21_000940"
     CDS             complement(21263..21811)
                     /locus_tag="IXT21_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4868364.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21900..23081)
                     /locus_tag="IXT21_000941"
     CDS             complement(21900..23081)
                     /locus_tag="IXT21_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ4868365.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23317..24825)
                     /locus_tag="IXT21_000942"
     CDS             complement(23317..24825)
                     /locus_tag="IXT21_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ4868366.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24984..25175
                     /locus_tag="IXT21_000943"
     CDS             24984..25175
                     /locus_tag="IXT21_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868367.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25378..26295
                     /locus_tag="IXT21_000944"
     CDS             25378..26295
                     /locus_tag="IXT21_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ4868368.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26288..27361
                     /locus_tag="IXT21_000945"
     CDS             26288..27361
                     /locus_tag="IXT21_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868369.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27509..28027
                     /locus_tag="IXT21_000946"
     CDS             27509..28027
                     /locus_tag="IXT21_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868370.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            28008..28400
                     /locus_tag="IXT21_000947"
     CDS             28008..28400
                     /locus_tag="IXT21_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ4868371.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28476..29411
                     /locus_tag="IXT21_000948"
     CDS             28476..29411
                     /locus_tag="IXT21_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ4868372.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29544..30323
                     /locus_tag="IXT21_000949"
     CDS             29544..30323
                     /locus_tag="IXT21_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ4868373.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30316..30633
                     /locus_tag="IXT21_000950"
     CDS             30316..30633
                     /locus_tag="IXT21_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ4868374.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30877..32487
                     /locus_tag="IXT21_000951"
     CDS             30877..32487
                     /locus_tag="IXT21_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4868375.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32644..33492
                     /locus_tag="IXT21_000952"
     CDS             32644..33492
                     /locus_tag="IXT21_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4868376.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33723..34202
                     /locus_tag="IXT21_000953"
     CDS             33723..34202
                     /locus_tag="IXT21_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868377.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            34235..36463
                     /locus_tag="IXT21_000954"
     CDS             34235..36463
                     /locus_tag="IXT21_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ4868378.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            36592..38235
                     /locus_tag="IXT21_000955"
     CDS             36592..38235
                     /locus_tag="IXT21_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ4868379.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(38308..38853)
                     /locus_tag="IXT21_000956"
     CDS             complement(38308..38853)
                     /locus_tag="IXT21_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ4868380.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38876..39361)
                     /locus_tag="IXT21_000957"
     CDS             complement(38876..39361)
                     /locus_tag="IXT21_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4868381.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(39351..40163)
                     /locus_tag="IXT21_000958"
     CDS             complement(39351..40163)
                     /locus_tag="IXT21_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ4868382.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(40302..>41411)
                     /locus_tag="IXT21_000959"
     CDS             complement(40302..>41411)
                     /locus_tag="IXT21_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ4868383.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13757 a         7488 c         8072 g        12094 t
ORIGIN      
        1 ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat aaaagccact
       61 acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt ccaataaata
      121 tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct gccattatta
      181 gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt cattttcgta
      241 tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga atttgtctga
      301 cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta tcttccaaat
      361 agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa aaaaggtttt
      421 tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc taattctttg
      481 atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata ttgtgtagta
      541 ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt gctttttttc
      601 ttcaagataa acgttttaat cgcttcttga gtttgcatga aagaggctcc tttttactta
      661 aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa aaaacagtaa
      721 acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca aggagtgaga
      781 gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc ttatcgctta
      841 ctataaatca gacagtccac acagaggaga cagaaacagc caaatggaca gaaaccaagt
      901 gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat tcaggagtcg
      961 caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat tcgggagact
     1021 tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccact ttgtcgattg
     1081 gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc ttcaccgtta
     1141 cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca gatggactac
     1201 cgaacgatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa gattcagcca
     1261 tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat gaaaataagc
     1321 cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata gaacctggag
     1381 aagaacccga tacagaaact caaccagagc cagacaataa atcaaaacct gaaataaccc
     1441 ctggagaaaa accagatgta actccagaac cagatacgga ttctggaaac caaacagtac
     1501 cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca gaggtagatc
     1561 cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac caaggaatac
     1621 cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt aaaaaatcaa
     1681 atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga atatttgata
     1741 gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct gtcctgaaac
     1801 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
     1861 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
     1921 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
     1981 aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
     2041 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
     2101 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
     2161 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
     2221 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
     2281 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
     2341 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
     2401 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtatt
     2461 taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
     2521 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
     2581 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
     2641 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
     2701 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
     2761 atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca tgtcaaatgt
     2821 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
     2881 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
     2941 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
     3001 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
     3061 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
     3121 cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaaga aaaaccgcta
     3181 aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc agtttcagcc
     3241 ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt gcagtgtatc
     3301 caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac cttctgcttg
     3361 tttttgcaac gcagcacctt gagcagtcag cgtaacattt acgattcttt catcacccgt
     3421 ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg gagtcagcgt
     3481 accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt tcttttccca
     3541 taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga ggaggggagt
     3601 gtagtgtcta atgatttctt tagaggcggc atataaagga aaacatactt gattaactaa
     3661 atatagatct tccatttttc cacctccgct gttttactat agtgtatcat agcggattta
     3721 cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc agctgtagct
     3781 tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc ttccagagaa
     3841 agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc actttctgaa
     3901 taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg ctgtttgatc
     3961 aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg ttcagggttg
     4021 gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt acgatcggga
     4081 ttatgcactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata catttttttc
     4141 ataaaattac cacctttaat tatattttat acaattgaat tttacacaat ataattgtgg
     4201 aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa cagacgcgct
     4261 ttctcgaata gcaaacatat acaaaaaata tcagagacaa aaaataatct catcataaac
     4321 attacatata tttctcaaaa agttgttttc tttcttcaaa aattacttca cgatggacct
     4381 gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt
     4441 ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg
     4501 tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag
     4561 gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgta ccatacaagg
     4621 taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat
     4681 caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca aaatcgattt
     4741 ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt
     4801 tattatacca gctaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt
     4861 cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa
     4921 aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca
     4981 caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg
     5041 taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac
     5101 tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg
     5161 aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa ataaacagat
     5221 taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat
     5281 attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata
     5341 aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg
     5401 aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaccaag cttccgaaaa
     5461 gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat
     5521 tcaacactaa aataactata gctcggtcaa agcctaaagc agcttccagt aatattccta
     5581 gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc
     5641 ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt
     5701 tcatttattc acttccttaa ttcattaata atactcttat cttattataa cttacttttc
     5761 accttaagtt aactttaatt aataaaaaaa ttaggctagt ctaaattatt atgtttcatt
     5821 cttctagaat atacttctac gccaaaaact gttaaaaaac ttttacttta gaaaattgga
     5881 gaaattgatc gcttaagata aattcaggac actaaatagt acaggaagta gactaaaact
     5941 tttaaaagtt ttaagtctat ctatttggca agtttaaaaa ataaaaattc ggtatcaaaa
     6001 aactgttacc gataacattt ttgaaagcgt tgtcttttgc ttctctatca cttatagtga
     6061 tgacgtcata ttaaaacaca aggaggaaat caatggatac agctgcaatt tatcatcgac
     6121 cggaaagtga atatgcattt ctatatacga atacaagatt tcgtattcgg cttcgtacta
     6181 ggaaaaatga tatcaaaaaa gtatatgtgc tatgtggaga tccatatacc ataacaactg
     6241 aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat gttcatgatt
     6301 attgggaaat tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc catgttgttg
     6361 gggaagataa cacggactgt ttttacggtg atcaaggaat ttttccttat cgagaaaatg
     6421 tgattactga accaaatttt tattttcgaa taccttattt tcatcaaatc gatcgattca
     6481 ctattccaga atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa agatttgcaa
     6541 acggggacaa agcaaatgac cccgaacaca ctttaccttg gggaagtaaa gatccagacc
     6601 gagaagactt tttcggggga gatttgcaag gtgtgatgga tcatttggat tacttgacgg
     6661 accttggcat caatggcatc tatttttgtc ccatatttaa agccacatcc aatcataaat
     6721 acgatacgat tgattattat gaggtggatc ccgcttttgg cgataaaaaa cttttaaaaa
     6781 acttgattga tgaagcacat aaaagaggga tacgtatcat gttcgatgct gtattcaatc
     6841 atatgggtgt ccattcacca cagtggcaag acgttctcaa aaacggagaa aaatcgatct
     6901 ataaagactg gttccacatc cgttttttcc cagtagattc ttatcagatg actgacttac
     6961 cagaaactgc tgaaaatatc ccatatgata cttttgcttt tactccattc atgccgaaac
     7021 taaatacagc aaatccagaa gtgcaaaaat atttgctgga tatcgcaact tattggataa
     7081 aagaattcga tattgatgga tggcgtcttg atgttgctaa tgaagtcgat catcattttt
     7141 ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga catctatatc cttggagaga
     7201 tttggcattc ctctcaagct tggctgcaag gggatgaatt ccatgcagtg atgaattatg
     7261 catttacaga ttcgattaaa gattattttg caaaaaagaa aatcacagct tcacaaatgg
     7321 tcagtggaat gaatcatcag caaatgcttt acagagatca ggtgaacgaa ggaacattca
     7381 acttattaga ttctcatgat actgccagga tcttgaccct ttgccaaggt aacaaagaat
     7441 tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt atctattacg
     7501 ggacagaaat agggatgact ggtgaagatg acccggattg ccgcaaatgt atgatatggg
     7561 agaaggagga acaagatcta gagctattcg gttttataaa agaacttgtt tctctaagga
     7621 aacagttgtc taagattatt tctgaaggtt ctactcagtg gctaatagta aatgatcgtg
     7681 aagataagct ctatttcaca cggaaactag agggacagat catctatgtt tactttaatc
     7741 agtcaaaaga accttgggta gtagaacagg aaaatgaggt gattcttagt caaaattgtc
     7801 agttgttaga agacgggaaa gcagagatac gacaatacgg atttttgatc atgtgttgaa
     7861 aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc cattataata
     7921 agggtgtaac agcacgttga atacaaagga ggaaatgaag atgaaaaaaa gaagtgtgaa
     7981 acagttgggt ttagggttaa tctctttagg aatcttaggc ggattagcgg cttgtggcaa
     8041 tggtaactct tcagcagaag gggaagccga tgaaggaaaa acgttaacag tttccgttga
     8101 ttctggatat aaagattata taaacgaaat aaaaaacact ttcgaaaaag aaaacgatgt
     8161 gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg gaatcattag cgttagatgg
     8221 gcctgctgga aagggacctg acgttatgat ggcagcatat gaccggatcg gtgcgttagg
     8281 acagcaagga catctggcgg aagtaaaact gggaaatgaa gcagcttacg atgaaacaga
     8341 caaagcccaa gtgacttatg atggaaaaat ctacggagaa cctgcagtaa tcgagaccct
     8401 tgttttgtac tataacaaag atttagtcga tacagcacca tcaacgttta aagatttgga
     8461 gaatctatcc aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa atacaggatt
     8521 tttagctaaa tggactgact tttattattc ctatggttta attgcaggtt acggtggcta
     8581 tgtatttggt gatgatggca caaatccttc agatatcggt ttgaataatg caggtgctgt
     8641 agaaggaatc tcttatgcaa cagattggtt caaaaatgtt tggccaaagg gaatgcaaga
     8701 cataaaaagt gcaggagact tcgccagcca gcaattcatg tccaataaaa ctgcagcaat
     8761 cattgacggt ccatggcaag cgcaaacata caaagaaaac aatatcaatt atggtgtagc
     8821 caaaattcct actctaaata atggacagcc ttatcaaccg tttggcggag gaaaaggatg
     8881 ggtagtcagc aattatgcta aaaacaaaga cctctctcaa aaatggcttg attatgtaac
     8941 gaatcaggaa aatcaagaaa aattctttga aatggtaaat gaaatccctg caaaccaaca
     9001 agctagggaa acagcaaagg ctaaaaacga tgaattaaca acagctgtga ttgaacaata
     9061 tgaaacagct caagcgatgc caaacatccc agaaatgggc gaagtttgga caggtgcaga
     9121 aaacttgatg ttcgatgcag catcaggttc aaaaacacca aaagaatccg cagatgaagc
     9181 agtaaaaaca atcagtgaag ccatagaaca aaaatactga ttaaaatgat aaccgtccat
     9241 ttttataaaa aatggaacag gtgcttcaga taaaagtggc aagagcgaga tggaaaatta
     9301 tcttgctctt gttgccatta tcttaaaagc agtttgcggt ttagcaagtt tagagggggc
     9361 attcagatgc agcaagataa aacaagcaat atcaacgaaa aaaaaatcac gctttgttca
     9421 cttattccag ggatggggca gtttgtaaat ggccaagctt ttaaaggtat cgtttttttc
     9481 gccatattcg tcctattcat tggtcagatg cttctgggcg gatttcatgc attggtagga
     9541 ttggttactt tgggaagtat accaatggaa gatcattcat tatttatttt gattgaaggc
     9601 actttgcaat tcatcattac aatcattttc ttgatttttt acggtttgaa tcttttagac
     9661 gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac gatgaaggaa
     9721 gtcatacata atataggtga tgaaggtttt ccttatctat tgacacttcc cgcttatctt
     9781 ctcatgattt ttacgatcat tttcccagtc ctagttacat tgttcactgc atttactaat
     9841 tatgacttca atcatattcc accagccagt ttaattgatt gggtaggggc taaaaacttt
     9901 tttagtatct tctttttaag ctcatatcgt aatacttttt ttgcagtctt ttcgtggacc
     9961 gtgatttgga ctttatgtgc aacaactttg caaatcaccc ttggtattct cacagcggtt
    10021 gttgctcacc agaaatttat taaattcaaa cgtattttcg gtatcatttt cttattacca
    10081 tgggcggtcc cagcttttat tactatcatg agtttttcga atatattcaa cgatagcgta
    10141 ggtgccatca atactcaagt gattcctttc ataaaccagc ttccttttgt agatatcagt
    10201 gcaatctctt ggaaaacgga cccgaactgg acgaaagtag caattatctt agtgcagggc
    10261 tggttaggat ttccctatat ctatgtgatg gttacaggaa tcttacaatc gatttcagaa
    10321 gacttatatg aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt ttcaaacatc
    10381 acgcttcctg ctgtcttcct agtggcagcg ccaacattta ttacacagta tacggggaat
    10441 ttcaataatt tctccatgat ctatctgttt aacaatggcg gtcccggaag cgtaggcgga
    10501 ggagctggtt ctactgatat tcttatttcc tggatctata aacttacgac tggaacttct
    10561 ccgcagtatt ctatggcttc agcaatcacg ttgattatct ccgtcatcgt tatctgtgtg
    10621 tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca atgaaaaaat
    10681 ttcattcgag tgctcgattc caaaaacgac tttctcactt ttttacttac ttttttcttt
    10741 taatactgtc agtcattatc atctatccgc ttttgatcac agcaagtact gccttcaagt
    10801 caggaaatat tgcagctttt agtttagatc tttcaagtga ctggacttta ggaaacttta
    10861 ctcggctatt cagtgaaaca ctttacggga cttggtataa aaatacattg atcatttctg
    10921 tcctaacaat gatcgtacaa gtgactgtag taactttggc gggctatact tatagtcgct
    10981 atcgttttat cggccgcaaa tcaagtttga tgttcttttt agtgattcag atggtaccga
    11041 ctatggcagc attgactgcc ttttatgtca tggctttctt attagaagcg ctcgataagt
    11101 actggttctt gacaatgatc tatataggcg gtggaattcc aatgaatact tggctgatga
    11161 aaggatactt tgacactgta ccaatagatt tagatgaatc tgctaaactt gatggggcag
    11221 ggcatttcag gatttttttc cagattgtat taccgcttgt caaaccgatg ctcgccgttc
    11281 aagcattatg ggcttttatg ggaccttttg gggatttcat gttagctaag ttcctgcttc
    11341 gaacacctga aaatcagact gttgctgtcg gcttacaaac atttatttct gatgtgacaa
    11401 accaaagagt ctctcttttt gctgctgggg caattttgat tgcactaccg atttgtctat
    11461 tattcttctt tttacaaaag aactttgtta ccggcctgac atctggcgga acaaaaggat
    11521 aggagcagag tatcatgcaa acatttccgt taaactattt ttccgcatta cgtacgccaa
    11581 ctcaagtgtt tgcgggacgc aaactattat catggcctaa atttttcttg atatttgtat
    11641 ttttagtttc cttgatggtc atgcctgtga ctctttttta tgccaatcag atccaagcga
    11701 ttccattaga acaatttcta tccgttcatt cgctgatcga tgaacaagga acccaaaaat
    11761 tttctgaact tgaattatct gaaactgggt tacaagcatc gcaacaaacg atagctgtga
    11821 cccctgaaat tttggtaggt gtgtctttat ctgagaaaca acaatccgat catggaactt
    11881 ttattgattt tgagaaggaa caatgggtga tccaacaaaa ggataaatct ggtatacgcc
    11941 gctatacgat gaattatagc ccatctttcc aaccagattc tgtacgtacg cccgaagatt
    12001 tccaacgatt tttagaaaga gaattttatg ccagtaatcg tccaacaatc attttatctt
    12061 atagtttaag tcttggtctt gtcctatttg tcatgacgag tctcatttta ttcggggctt
    12121 cctttttctt gtggatgaca agaaaaagcc aactttcatc cattcataca ttcaaagaga
    12181 gtgcaaattt gatgctgaat gttatgggaa taggttccat ggcagcagca gttgtagggt
    12241 ttatccactt tgactttatc ttgatgctaa gtgtccaaac aacgataact gtcttgctgc
    12301 ttttatgggt ttttgtcaaa acaagattca aagataagcg tgtcgaatga caagtttaag
    12361 cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt gaaaaatttc
    12421 tgaaaaaatt aaacttctta gttgattctt ttctttttta gtattaacgt aatagggtag
    12481 tttttttaag gaggaaaatc atgttagaga aaactttata caatcaatta ttttcacgtt
    12541 ctttctcgct ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa tacggagata
    12601 cggacactcc tccccaaatc aaaatcaata tccatgaaga aatcccaatg aaagagatta
    12661 caagtaacgc atctttggca ctaggagaag cctatatgga tcatcgaatc gagatcgagg
    12721 gaagtatcca agcattgatc aacgatgctt atcagcaagc aaacagcttt ttacgaggaa
    12781 aagattatct aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa aacaaagaag
    12841 atatccatgc gcattatgac ttaggtaacg acttttataa actttggcta gatccaactt
    12901 tgacttattc ttgtgcttat ttcaatacac ctgaagatac gttggaacaa gcacaaatca
    12961 ataaagtcca tcatatctta gacaaactct ttatcaaaga aggcgaaacg ttgcttgata
    13021 tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa agagtataat gtcaaggcaa
    13081 ctgggatcac actaagtgaa gaacaatacg atttgattac caaaagaata aaagaagaac
    13141 atttagaaga taaatgccgt gtattattaa tggattaccg cgaactaaaa ggcgaaacgt
    13201 ttgatcatat cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat cttgaaggtt
    13261 atttcaaagt ggtaaaagat ttattgaaac caaaaggaac agctttgatc catggaatca
    13321 gccgacaaca aggcggtgcg acaaatgctt ggatcaataa atatattttc cctggcggtt
    13381 atgtccctgg tgttactgaa ttagttgata atattacaaa aaacgatttg caactgattg
    13441 atttggaaag cttacgtcgt gactaccaat tgacattaga acattggaca aagaattttc
    13501 atcaagtcca agatcaagtc acgaaagaaa tgggtgattc cttctatcga atgtgggatc
    13561 tatatttaca agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg attcaatatt
    13621 tgcttgttca acctggaaac aatgacatcc caatgcatcg tgcataattg aggcaaaagt
    13681 actgtttaaa gactatttaa aaagaagcta agttttatga tgctccagaa gtgaactttt
    13741 ttgtctaact tctaggattg tctctaaagc ttagtttctt tttctttata ttttcataat
    13801 aaaagtttgg ttctttaatt tttaaacatg acagaaaatt ttttcttcgt tatacttgta
    13861 atagaatact attttataac aaaggggatc gaatcaatga aaaaaactgt tatttatact
    13921 agtttatttg cagctttgtt tttagctgct tgtcaatcaa ataccaatca aggcacatct
    13981 gggaatacgt cttccagtag cgaacagcgc tcttcacagg tatcggactc ctcaacaaaa
    14041 gcaagcacat cttcgtctag ccagacaaaa agttctagtg aagcaagtac tcagtcagat
    14101 atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc agatactgca
    14161 attactagtt tggaattaga tagtgattgg ggatcttact tctacaagat agaaggtgtc
    14221 gatgatcaaa aagaatacga agcaagaatt gatgctcaaa atgaaaaaat ggagacagag
    14281 aatccagaaa ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga agatggtctg
    14341 gatgttgata aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga caaagtagga
    14401 agcggtacgg caacagattg ggatttggat aaagatttag gtactactta ttgggaagtc
    14461 aaagtaaaag acggcaatca aagtacatca gtcaagatca atagccaaac aggcgaagta
    14521 ctgtcaactg aacaagatga ttaagaattt cttcggaaaa caagagttga tttttctttt
    14581 agtcatgata cactagacaa aataacttgt ttgaaggagc gatttacttg agtttagaaa
    14641 cgacctttgc caatcatatc tttgccaatc ctttgatgaa tgcatctggt gtccactgca
    14701 tgactaccca ggaattagat gaactggctc attcagaagc aggagcattt atcactaaga
    14761 gctgtacgat caatgaacga aaaggaaatc ccgaaccacg ctattttgat gttcctttag
    14821 ggagtatcaa ttctatggga cttccaaact taggtttttc ttattatcta gagtatgctc
    14881 ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc attgccggga
    14941 tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg tttaatggga
    15001 tcactgaatt aaatttatct tgtcccaatg tgcctggaaa accacaacta gcttacgatt
    15061 ttgaggccac ttatgaaaca ctgaaagaag tattcagcat cttttctaag ccgctaggaa
    15121 tcaaattgcc tccttatttt gattttgctc attttgatca gatggctgat atcttgaatc
    15181 agtttccatt gacctatgtc aatgctatca acagtgtggg caatggtttg tatatcgata
    15241 cagaacaaga agcagtagtc atcaaaccaa aagaagggtt cggcggcatt ggcggagagt
    15301 atatcaagcc gactgctttg gcgaatgttc gcgcttttta tacacgtttg aaaccagaaa
    15361 tccaaatcat tggtacaggt gggattcgga caggacaaga tgcatttgaa catctgctat
    15421 gtggcgcaag catgttacag attggtaccg agcttcataa agaagggccg gaaatttttt
    15481 ctagaatcat aaaagaatta acgcagatta tgtcagaaaa aggatatacg tcaattgacg
    15541 aatttaaagg caaattacgc accatttcat agcaaagaga cgaaggtcat gattgatctt
    15601 cgtctttttt tataaaaaac actcgtctgt tcattcctgt gcaagcgtac tcattgtatg
    15661 ttagacgaac ttgtgggaaa ataacagagg caaaaaggag gataatatga atgatgcaac
    15721 agaattagca aaagagctta aagaaaaaga atttagtttt caagaactag ttacggatat
    15781 acagaaaaaa ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc ttgaaccatt
    15841 cgagagtaat tcccacgagc tattgattcc ccagtcagaa aaagggccat tggctggtat
    15901 cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta cttcaggatc
    15961 tcgtttattt gaaacacacc gagcttctca tacatctaat tatgtgcggc aagcagaagc
    16021 gataggtcta gttccttttg gacaaaccaa tgcgccagag tttggtttca aaaatattac
    16081 agacccagtc atctatggcc cagcaagaaa cccttggaac cttgaatgta caccgggcgg
    16141 atcaagtgga ggagctgctg ctgcagtttc atcaggaatc gttcctttag ctggtgctag
    16201 tgatggcggt ggttctatta gaatacccgc ttcgttttgc ggattgattg ggctgaaacc
    16261 ttcgcgaggg acgatgccag ttggtccata tgcttggaga ggatggcaag gagctgctat
    16321 tgatttcggc ttgacagttt ctatgagaga cacggaggct ttattttatg ggatgagaag
    16381 tatccattcc ggtgcgccat atcaagtatc gccagttgag tggcaaacac atccaagaaa
    16441 aaaacggttg aagatagctt tttgtgttga ttcaccgatc cactcacaga ttagtgaaga
    16501 ggcagtactt gctgtaaaaa aagctgtaga tttccttgga aaagaagggc atgatttgac
    16561 agagattcct tacccactag atggtcgtaa actgatcacg tcttattatc agatgaatgg
    16621 cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc atccagcgag ctgtcagaaa
    16681 agaagaactt gaacccattt cttggacttt gttccaatat ggtcagaagc tctctgccgc
    16741 tacttacatc gattctctgc atgtatggga tgaagcagca attgtcatgg aaaacctaca
    16801 tcaagaatac gatctattcc tttcaccaac gactgcattt accgccccta aaatcagcga
    16861 agatttgcaa agtgatgcga taagagcaca gatggaacac atcagtgatt gttctgaagt
    16921 agaaggttta gacgttgttt cttccatgtt tgaggagagc ttgaagatca caccttatac
    16981 acagttggct aatctgactg gacaaccagc aatcagcttg cctactcatt tgacaaaaga
    17041 aaacatgcct ctaggaatcc aatttatggc tgccagagga agagaagatt tgctatttca
    17101 aatcggctat ctatttgaag caaatcatca gttccattta ccaaatccat ggaaatagca
    17161 gaaacaagga gaagaagaat gaagaaaaaa agattgtttg ctctaataac tagtgccttt
    17221 tttattttaa gcgcttgcgg tacagctgcc gctaatcagg aagataacag ctggagtaat
    17281 gtagaaaaaa agaaagaatt agtagtagcg acttctggca cactgtttcc cgcatcttat
    17341 tataatgatc aaaatgaatt agttggttat gatgtagatt tggcaaaaga ggtggctaaa
    17401 cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat cacttctgtt
    17461 tcaaaaagag aatcagattt tgctgcgaac gattttggta tcacaaaaga aagagaaaaa
    17521 aaattctccc tttcagagac tttcaaatat tcatttgaca gtatgatcgt acgaaaaaaa
    17581 gatgattcag gaatccactc acttgaagat cttaaaggga aaaaagcagc tggcgaaccc
    17641 aatactagct atatgcgttt agcggaaaaa cacggagcta aattagtcac atatgacaat
    17701 gccacgaatg atcagtattt aagcgatgta gcaaatggtc gaacagatgt tattctaaat
    17761 gattattatc tgcagaaaat ggccgtatct gctttaccag atatccctgt aaaaatactg
    17821 gagaacgttt atttcaatga aaacgaaagt ggttttttat tgaaagaagg aaatgatgct
    17881 ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag aagatgggac gacaaagaaa
    17941 atatctgaaa aatattttca aactgatgtt tcagtcaaac cgaaagaaac gatggctgag
    18001 tcggtgagtg agtgatgtac tttcctctaa ttgatttttc cctcatgtgg acttcacttc
    18061 ctttcgtact gcaaggcttg gtatacacat tagggattgg cttcgtgtcg tttcttttag
    18121 gaaatctggt agggttgctt ttgacagttt taggtctttt ggattggctg cctctgaatg
    18181 tatttattcg tttttattta tcgttcttta gaggcattcc cgcactagtc ctgctgtttc
    18241 tgttatattt tgggttacct tatcaattaa gtgccttaac ggcttcagtc atctgcttta
    18301 cgattaccag cagtgcattt attggtgaaa tctatcgagg atcactagca ggcttttctt
    18361 caggtcagtg ggacgctggt ttagcttctg gtttttcttt ctttggggta ttgaaatata
    18421 tcgttttgcc gcaagctttt cgaatctcag taccggcact cagtaacgta gctatggatc
    18481 tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc agaaatattt caaaaagcaa
    18541 agatcatagg cggtcggaca ttcgattatt tttctctcta tattttagtt gcactgattt
    18601 attgggtcat ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa cgttctatgc
    18661 atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt ctattttttg
    18721 tttcttgtat attctgccta gtattttaaa accatttcat gtaaaatgta aatcgaaaat
    18781 atgttcggag gtagaaaaca tgaagacccc gctaaccatc gaaatagaat cctcgttata
    18841 ccaatattgc atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca
    18901 aggaattgta gatacgctta gctgcttgac aaaatttgat ggaagccgag aatggcgatg
    18961 ctatgagctg aaagtgacaa aagcagattt tcgctctaca gccaaactgt catttgttgg
    19021 acattataat tattttgttt taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc
    19081 tacaggaata ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc
    19141 tggtacattt ctgattgcga aaaaagcagc aaagaaagag cttgctgtac cggaagaagc
    19201 tttgacaaac cgcttcatgg cttctttatt cagggaagta agaaaagcaa agcaaatgga
    19261 gcgaggcgta aaatattttc caacagatca attgtacaaa gaattgaaaa gacgttcaga
    19321 aaatcaagat ccattttcta acgaacgata ttatgaacgg tttttagagg aaatagctag
    19381 tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt cagcatgatt atgaatattt
    19441 gaggcaacaa cagcagatcc gtcggttgcc tacagagcca ttggagtgaa aaatatgtac
    19501 tatccttatt taagaggaaa acaatttgac ctattagcat taaaagaagc actgtcgcga
    19561 ggattgctct ccaataaaat ccaaccagtg atcgaaccag tacgtgattc cgctacgctt
    19621 aaaaatgtga tcgaattatt tcaaaaaaag caccatcgaa tcgctgtgat ccaaaatccc
    19681 caagtaggac aattcaaatt attcgatcag catgtgcaca gttggacagt caaagaaaat
    19741 agctctgtcg tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt actggattca
    19801 cctccagcgt ttcttgtttt cgatggacag cactacccaa aagatacaga ggtgtggaaa
    19861 cagttggcag gagttgattc gaaatttttg ataccagata ctagtcgctt tcgcatctgg
    19921 ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt
    19981 tatgcagata aaacagatga cttttttagt gatgactatc tttttttcca cgcagacgga
    20041 tatattggat tttctgattt cacgatcgaa ggtagccgtt atttcgataa agggttccct
    20101 agccgagcgc ttgctatcca tctaacgtat attgatgctt acggcaatat acgcgtgaaa
    20161 cattttgtct ctgacagtaa cgactctgct aaagaccaag cattgaagtt tcttgaagca
    20221 ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc aactcttgat tacttcagga
    20281 atgattgaat tattggctct ttaccagcaa caaaaattcc ctggactggg agtgttgaaa
    20341 aagtggtcac ttatgcatca tttggaactt attagtcacc tgctagatca tccaaaagat
    20401 tggcttaaat attattcaaa agttcaggaa ctatatgaag tgatccaatg attagataat
    20461 gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat attcggcaaa caaggaaaga
    20521 agaagtagaa gaagtggtag aaatcattaa tgccgcagcg gatttcattg cttcgcaagg
    20581 ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa acgattttga acgatattga
    20641 aagaaaagaa agctatgtat taatagtgga agatcaaatt gctggaacag ctgcattagt
    20701 ctcaagcgtg gatcccgttt acacaaaaat agatggtagt tggaaagaat caactggtcc
    20761 ttatttatcg attcatcgtg tcgctttagc agaaagtact cgaggaaaaa aactagcaaa
    20821 accgttctta gaacatttgt tgacgattgc agccagcaaa aatatccaag atatccgtat
    20881 cgatacttat ccagctaatg aaccgatgga aaagaccatc tattctgctg gattcactta
    20941 tcaagggatg attgaatttc cttttgaaca cggcgaaaga aaagcatatc aaataatcat
    21001 caattgacta taaaatcatc tgagcaaaga taaagacatt aacactactg aaaaaaacaa
    21061 tcatatgata gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt
    21121 tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt
    21181 ctgaaactcc cttcatcgag aggtcgtgac atagcttttg tcacgacctc tcggcttatc
    21241 ctacgagtat tattcgatac atttatatca ataatccttt ttttatttta tctgcagctt
    21301 cttggaacgc tccatcgttt gacacgacta aaagtgaggg gatagctttc aaacgtttca
    21361 actgtgctaa atcttctaca taaaccgcca gtctggctga aatctgttca gatgaatctc
    21421 cacgtttttt caggcgcgat tgcaatgttt ctttagagat cgtaataaag ataggacgga
    21481 ctaccgtccc gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac
    21541 aatttcctac tgtcagtttc tttttaattt ctgctttact ggaaccatat ttatgttgag
    21601 catattggtc ccactctgca aactcatttc ccttgatcat ctgattgaat gtattgtcag
    21661 aaacaaaata ataatcctct tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag
    21721 taatgatctt ttgttccggc tgaaaaatag cttctgcgag tgaagttttt ccagaaccag
    21781 aaggaccgat aaatacatag caataattca ttgtcatttc tcctttgatg attgaacaag
    21841 tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt aactttgctt ttttgtatat
    21901 tataccagca agccagtcac aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa
    21961 attttcaatc cgtgttttgc gacaagattt cctttttctt catttaatcc cttcatcgca
    22021 ccagaaatga tcccgataga agagaagttg gcaaatgaaa cgaggaagac agatataatt
    22081 gctgttgtac gctcagtaaa atggaaatta ccatttgcta attctgtcat agcaacaaat
    22141 tcatttgtaa ccagtttagt cgccatgata ctaccggcat ccactgcttc tttccatggg
    22201 attccgctga tgaaggcaag aggggcaaag atgtaaccta ggatctcttg gaaagtgatg
    22261 ccgaatatcc cgtggaagat agcattgatc atggcaatca aagcaacaaa accgataagc
    22321 atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc caacatttgg
    22381 aaaaatgtct gttctttgtt ttcttcaatg atcagctcat cttctttttc ttctaataca
    22441 tagggattta caattgaagc cagaatataa ccgccaaata agttcaaaac aagggcagtg
    22501 atcacatatt tagaatcgat cagtgccata taagatccga cgatcgacat agaaactgta
    22561 gacatagccg atacactcat ggtgaacaaa cgtttctcag ataggaaggg taattccttt
    22621 tttatggaaa taaaaacttc cgattgtcct aaaatagcag aagctactcc attataagat
    22681 tccaatttgc ccatcccgtt gattttactt aatcctagac caacagcttt cataaataat
    22741 ggaagaatct tgatatagcg tagaataccg ataatagcag aaataaatac aattggcatc
    22801 aatacggaaa gaaagaaagg tgcacttcct ttatttgcta caccggcaaa aacaaagtca
    22861 acaccttgcc cagcaaaagc aagaagatga tcaaacattt ttgccaatac agaaacaacc
    22921 gctgtaccaa aagttgtacg taataatacg aaaccaagaa caagctgtaa aacgaacatc
    22981 acaattagtg gtttcaattt gattgctttt ttgttactgc tgatcaaaaa agcaagccct
    23041 aatgtgacaa ataaaccaat aatacctaat aaataactca aataatcact cctattccaa
    23101 attccgtttt attttacctg ttttgcaaat aaaatactaa tctttagtga aattttcatt
    23161 acttttttag agtgaaacgc attcattttc cattagaaaa taaagcgaac aaaataaaag
    23221 gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc gattaagcta
    23281 atagtttcgt tttcaacatt tataagtaaa ctttttctat tggtaatata gattttcgga
    23341 agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt
    23401 tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata
    23461 agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt
    23521 gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc
    23581 aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc
    23641 atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac
    23701 ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg
    23761 aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc
    23821 gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt
    23881 gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac
    23941 aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg
    24001 ataaacagat tctttaccag taattttttc aataatacgt ttgattacag gaaatgtctc
    24061 aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat
    24121 catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt
    24181 caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt
    24241 ttggctttca tgatacagtt ggttcaagga agtagcgagc tttccacttc ctgggccagg
    24301 agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc
    24361 ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac
    24421 cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata
    24481 gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg
    24541 caagatatcc atatcataag taatcccata atcaccacgg atcttattac gttcaatatc
    24601 tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat
    24661 tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactagctt
    24721 cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga
    24781 ctgctcttca atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta
    24841 agtactaaag cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc
    24901 aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata
    24961 gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac
    25021 actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac
    25081 aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca
    25141 ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt
    25201 cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt
    25261 ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa
    25321 tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg
    25381 aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac gaatgcggat
    25441 caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat
    25501 ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc
    25561 cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat
    25621 gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa
    25681 tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc
    25741 acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt
    25801 ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac
    25861 aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta
    25921 gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc
    25981 ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta
    26041 tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa
    26101 aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa
    26161 gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat
    26221 gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt
    26281 tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga
    26341 tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga
    26401 agaaatccct atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa ctccgacgaa
    26461 tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga
    26521 aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt
    26581 agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc
    26641 atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat
    26701 cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa
    26761 ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca
    26821 aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc
    26881 gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt
    26941 gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat
    27001 attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa
    27061 tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt
    27121 gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat
    27181 ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat
    27241 aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc
    27301 tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg
    27361 attggataag ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc
    27421 agttatccag aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact
    27481 tgctgatgaa ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt
    27541 attgtctgct gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc
    27601 aatgactgct atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt
    27661 catgagtctt tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg
    27721 ccaatggatc agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac
    27781 agtgcaaagg aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg
    27841 aataggtatg accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta
    27901 ccttcaaggg gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt
    27961 ttttctgcca gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa
    28021 tttatagttt gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct
    28081 atacacaatc ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca
    28141 cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa aaaggcgatg
    28201 atttactgag tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt
    28261 cgcttgaagg gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg
    28321 ggaaaagtgc tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt
    28381 tcaatttgtc taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat
    28441 tcagtgagtt cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa
    28501 tgttttccgt aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg
    28561 gctatgcaat cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt
    28621 gcagcaatca gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt
    28681 tgcatcaatt tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc
    28741 ttttcttcgg cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat
    28801 cccatgaagt gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg
    28861 aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt
    28921 ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag
    28981 acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa
    29041 ccgggattgc tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag
    29101 ccgccatttt cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa
    29161 gcgatttatc aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata
    29221 taaaaacagt tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg
    29281 gtaacaaaga gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt
    29341 ctcttcctga caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt
    29401 ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt
    29461 tttaaataaa acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg
    29521 ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt
    29581 tgcatgcagt gatgccactt tgtataagct atataccagt aggattagct tgcggagtat
    29641 tgttgcaaaa agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt
    29701 ccggtggcgc gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga
    29761 cattattgat ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg
    29821 gttttatgaa aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg
    29881 aaaactatgc ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg
    29941 ctttatatgt caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt
    30001 ttttcggctc ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt
    30061 tcattttcat gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct
    30121 caggcgtatt aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag
    30181 caacagtcat tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag
    30241 ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac
    30301 aggaggccaa tcaccatgac taaactatat ttgctacttt tagttgcttg tctgttcatc
    30361 gtggcatata tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc
    30421 tggttcagtg actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa
    30481 gatgttttca ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg
    30541 attctagtag caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga
    30601 cttgcgtctg tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga
    30661 ttgcgacata agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg
    30721 atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt
    30781 ttattttagg aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga
    30841 tgtttatttt atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga
    30901 acattttggc taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt
    30961 ccctggctaa gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat
    31021 gttggctatc ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg
    31081 attgcaaaaa aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt
    31141 tttagaagta cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat
    31201 ttatttgctc ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta
    31261 gctatcagaa gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga
    31321 tatttccaag gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct
    31381 gtgcgagtga tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc
    31441 atattagaag ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt
    31501 atccatatgt atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt
    31561 tccagccgtt atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca
    31621 ctaccgttta tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg
    31681 atgaaaccac tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta
    31741 ttcagccgtg cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga
    31801 acagtagcta taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa
    31861 atcgaaaaaa cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg
    31921 actggaatgt ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa
    31981 agtgtcggat atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc
    32041 ctatctacga tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa
    32101 gcaattatcc tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg
    32161 atgagcttat caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg
    32221 tgcaaagaat accgaatagc acctttagca aagatcagca attttttctt aaaaactttc
    32281 cgctccaccc tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga
    32341 ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc
    32401 ggcttagtat ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt
    32461 aaacatcata gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa
    32521 acggcaactc gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct
    32581 aatcttactt atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa
    32641 cacatgaaac ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac
    32701 catcccgact tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa
    32761 ggaatcgata tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct
    32821 actacttcac aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa
    32881 gggtatgaca cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca
    32941 ttatttgcga tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc
    33001 actagtatgc cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag
    33061 tcccttgatg aaatacttgc acatatcgat cgaatccgag ataataccta cgcttatctg
    33121 attgtagatg atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg
    33181 attggcagct tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta
    33241 ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga
    33301 agaaataacg aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa
    33361 gtagcgattg cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt
    33421 ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt
    33481 tcagcgcaat aattcctttt attcaaaaaa cataccaaaa atattccaac atcatcttgg
    33541 actttctaca tgttactaat gagcgtgtaa aagaacgaga tggtgttttt atttctatca
    33601 atgaagctct ttttctttgc aatcctcact tcaatcctct ataatgtcgc atatagttta
    33661 gtaaatgagt aaactactaa tttacttatt tactaaaaat aaaaggatga ggagttaaaa
    33721 taatggaaat accaacaaat ttaaaacata aaccagtaat cgttgtagaa gactatgata
    33781 aagttgatgg aagaaatgca ttaaatacgg atgcaaaagg gctttcactg ggccttgctc
    33841 agtggaatga tcgtggaaaa gtcgatatct caggcaaggt atggcgacat actggagaaa
    33901 aatggtcacg acagtcggaa gaattaccaa tcacacgtat tttagattta gcgatcttag
    33961 ctgcacaagg aagtttatac ttccaagatg cttatcgtta tgaaaaattt tatgatccag
    34021 aaaatccagt cgtagacatc attggattac aagggaaccg aatgacggta gaagtagata
    34081 ccaaaaatcc aaagatcgat gaagacatca ttttgtatta cgatacacta caaaaagacg
    34141 gagaactgat tggggaacga ttccgtattt taaaacgtct tttagatgag ctaggatatt
    34201 aatagacaga gatgatcaaa aggggaagag aagcatggag aatttctcac taacagaaaa
    34261 ccgaaccttt tcacggctag aaaagcaaat gatctggaag aaaccgaaaa gccatcggac
    34321 aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg aattggcaag atcctgaaat
    34381 gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg gggaagacac aattagccaa
    34441 agcactatct gctgatctgc aacttcccta tacaaaagtg acctgttttg cagatatgga
    34501 caaatcagat gttttcggag ccttattacc tgtagtagat tctaataagg aacaagacca
    34561 agaattacta gaagctattt accaaacaga tacacttgaa gctgtactga cactgatcca
    34621 gaaccattac ggcattgatc ctttttctgc taaagaaaaa ttagcagagc tagttcaaag
    34681 gatcgaagct gattatccag atgcaatcca ttacagctat tacccttctg aaatcgttcg
    34741 agcgattgaa aaaggatatt tattagagat tcaagagccg actgtcatcc gagatgcctc
    34801 tgttcttgtg gcacttaact cagcgctcga accaaatggg atgttgaatt taccgacagg
    34861 aatcattcgt cgccatccag attgcgtcat tgtgatcact acaaatcgaa attaccaagg
    34921 aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa aaatggatct
    34981 tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc aaaaacaaga
    35041 acttctctta aagatggcag agattatccg tttgttggat gaaactgcaa aagctaacgc
    35101 catcaaaggt gtagcaggaa tgcgttctta tttttattgg gcaaatacct ataagcaagg
    35161 acaagattta ctccagtcga tttatccaaa agttttgtat aaattatcca ctgattcaga
    35221 tgaattggca attcttgaac aagctttgtc tgacagtgga ttgctactgg aacttcagga
    35281 catattgaga aaagaaaaat ggggtcatcc tcaatctgag catacaaaag gaagaaccat
    35341 tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa ttctgcgctt
    35401 agatgaaaag aaatcgttgt ctgctgaaga accaaatgag ggagaagaga aacaagaaga
    35461 aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta gcgatatcga
    35521 aggaaaaaat caaaaagaac aacaaacagg agatgattct caagaaatgt ctgcttctga
    35581 catggaagca catgataagg aaatcaagaa gcaactgaat aaagaagcgc gacaaatcat
    35641 gaaggaaacg attcatgaaa aagaaggtct catcgttcac cgaccagctt tccaaggaaa
    35701 aacgccggaa gcacgtgagc tacacatgca agtaatgcca atagtagaat cacttagccg
    35761 gcaaatttta gagttactgg ataatgaaca aacagagacg taccaaaaag gaaaatacga
    35821 aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt ttgacaaaaa
    35881 gaacccgcca catgagcaac cttctttagc ggtagctgta cggatcgatg aatctgggtc
    35941 aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca ttgccgaatt
    36001 ttctaagaaa gtcgatatcc cattgttgat ttacggagat acagcagacc gttcgtcaag
    36061 agaaaagact tctttgtttt cttataaaga atttgaagac ggatttcaat ggttaaatga
    36121 aaaattagtc actatgaaac caaggcaaaa caatcgtgat ggtgctgcac ttcgtttagc
    36181 agcagaaaaa ttaaacagac aatccgcaac aaccaaactg ttgcttaata ttagtgacgg
    36241 tcagccaaaa gcgttgccgg attatacagg caaaaaagca aaagaagata tccagtctgt
    36301 tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc gcagctattg gccaagacaa
    36361 agaagaaatc aagtcgatct acggtgaaag tcgttttgta gatattactg acttgaatga
    36421 atttcccaag caaatgattc aactgattgc ccgctatcta tgataaaata aagctcaacg
    36481 aacgcactta gcagattcgt tgagcttttc gtttctagtt accggtaaca aggatacttc
    36541 taaaatagaa tcggttataa tcaagaggga ccgttaaagg agtgaaacga tatgatacaa
    36601 gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct atcctgctag tttcaaagat
    36661 agcaatggcg atggtcaagg agacatcaat gggatccgtg aaaaacttaa ttatttgaaa
    36721 gatcttggta tcggctttat ctggatcaat ccgatctatc aatctccatt tgtcgataat
    36781 ggttatgacg taagtgacta tcaagatata gaagagagat tcggaacgat ggaagatttt
    36841 gatttattgc taaaagaagc acatgaatta gggatcaaag tgattatgga tcttgtcatc
    36901 aaccatagtt cggatcagca tcaatggttt gaagaatcga aaaaatctaa agaaagcccg
    36961 tatcgcgatt attacatttg ggtcgacggg gtagatggca aagaaccgaa taattggaca
    37021 tcgattttcg gcggttctgc ttgggagtat tctcatgaaa ctggacagta ttatctgcat
    37081 gtttttgcaa aagaacaacc agacttaaat tgggaaagcg agaaattgaa agaagaacta
    37141 ttcaatatga tccgatggtg gttggataaa gggatagatg gatttcgttt agatgcgatt
    37201 tctcatgtaa aaaaagatga atattcagta aaagccacag agaacccttt ctcacccttt
    37261 caaaatgtct caggaatcga agaacatttg accgatttga aacatgtttt tgaagagtac
    37321 gatatcatga ctgtcggaga agcaagtggt gtcactgcag aagaaggacc acaatgggta
    37381 gggaaagacg gttatttcga tatgatattc gaatttgatc atatccatat ctggcagcaa
    37441 gaaaaagaag ggcaattaga tgtccttaaa ttgaaacttg cattaagcgc ttggcaaact
    37501 tcattggatg gtatcggatg gaacgcttta tatatggaaa accatgatgt tcctcgtgcc
    37561 gtttcagtgt ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa agcaatcgct
    37621 atgatgtatt tctttttgca aggtacgcca tttatttatc aaggacagga aatcggaatg
    37681 acgaatatgc cctttgaatc gatcgatcaa gttgatgcag tagattcgaa aagactttat
    37741 aaaaggctgc ttgcggaagg taagacaaga gaagaagcat tggatatcat tcgtgaaaca
    37801 acgagagaca acagcaggac acccatgcaa tggacttcgg aacaatatgc tggtttttct
    37861 acacatgaac cttggctgat cactaatcca aataccaaaa cgatcaatgt agaacaacaa
    37921 gaatatgaac cagagtcggt cctgcaattc tataagaaca tgatccgcat tagacaaacc
    37981 aataaaggtc tgatatacgg ttcttataaa gaatatcttc atgaacatcc acaactttat
    38041 gtctatgaaa gatatttaga agatgaacaa tatcttatta tggtaaactt aacagaatca
    38101 cttgctgact atgaacttcc aaaagaagca gatcagtcct ggacattact cttgtcaaat
    38161 tcctctagcg gagaatttga agctaaaggc atactagcac cttatgaagc tcggttatat
    38221 aaaacaaaca agtaaaactt cctcgttaaa gttcattagt tggactttgc gaggaagttt
    38281 ttcagattgc tctgtcaatt actcatttca ataattcgct ctaggaccac cgatatattt
    38341 aggacgagag cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac gaggtctgac
    38401 aggtacttgg acatgtttga tatgcatacc gattgatgtg tcaccgatat cgataccggc
    38461 ttgtccaact acgtgttcca cttcgacagg atcattgaac tggtcaaaag cagcaacaga
    38521 acaagcacct ccagcatgta gtgcaggaac aacagagaca atttcgaaat tttttgcttc
    38581 tgccactgtt cgttctacta ctaatgctcg gttcaaatgt tcacaacctt ggacagctag
    38641 cgcaatttct ttaggatcca acaactcttt taacgtacgg atgatccatt gaccaacttc
    38701 cgcactggaa ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc atcctaaaac
    38761 aaaaagatca ccttttttca agtgtgcctc agccaatatg tcattaacta tttcagtcaa
    38821 ttggtctttt aattcttttt ctgacaactt cattttctaa aaccttcttt ttcaattaat
    38881 attgcttccg tgcttgaata cgttgaagac ttgcagttat aggaaaggca agtcccattc
    38941 caatcagatt ttggaagatg tttcctggta tagaagccac tccagccggc caatcatata
    39001 aaatccaacc ggcgatacca tagccgatga tcataactaa gcttcctaat aaaagcccta
    39061 atccccacat acgagtagaa gatttgctga aagaaccagc aacatatcct tgtaacccgt
    39121 ggatcactaa tgaaaaaact gcccactgcg gatatcctga aatcaaatcg atcaatagac
    39181 cactggaagc accaacaact aatccacccg ttggaccata aagaaaagca gcggtataga
    39241 tcccggcctc gcataatgtc acaaatccat tcgtagccgg tacagggatc aaaattgtta
    39301 atgaaagggc aactgtcaac gcaatcagca tagcatagat cgttaattgc ttagttttca
    39361 tttctcattc ctcctttttg aataccgcca gaccagacac ttccggtatg atcgttcatc
    39421 agcacgccat tagaaataca atgggcaaca aattttttac tttctgctac tgctttggta
    39481 aaatctgccc ctaacacaag attcgctgtt attgcagaag aaaaggcaca accggccccg
    39541 tttactgttg tagtgtcgat ttttttatga gaaaatacct cataatgatc aattgaatag
    39601 agaagatcca cagcttcatc tccagccatc cttgtgccgc ctttgatcac aatgtttttt
    39661 gttcctgaag cagtgagttc tttagcgaga tggatcattt ctgttaacga agagggaaga
    39721 gcttgaccag aaagtaaagc agcttctgcc aaattaggcg taatcatcgt aacaagtgga
    39781 aaaagttctt cgatgagctg cgaaatatac aattgattga tcacagcttc agtttctttg
    39841 aatgccaaaa cagggtctaa aatgattggg atattttgtt ttgtttttat gaagtctcga
    39901 acaaccatga ttgcttcact tgaattcaat aacccaattt tgataccatc taatgaagtc
    39961 attttatcaa tcgaaactaa ctgtcgttta agcaattcca aagatataga ttccagcata
    40021 aatcgctcat taatatctag tgcccctata gaagtcaagg cgcttagtcc gaatagacca
    40081 aaattctcaa aagtttttaa atctgtttgg attcctccac cagcaaatgg atcagaaccg
    40141 ccaattgtca agattctttt cataacattt tcctccatga ataagagtat aacgaaattt
    40201 tcaaaaaaga aaaacagcca attggcatat ttttatctca tccaatttaa aaaaacaaaa
    40261 agaaaagaaa gaccaaaatg gatctttctt tccttaaata gttaggactt ttgtgatttt
    40321 aattctttct cgatttgaag ccgttgcact ttcattgtag ctgttcgcgg gattgcatca
    40381 aaatcacgga tgatagggac gttcaagcga ggaagttcat ggacttgctc ccaccatcga
    40441 ttccaatcca tttctttatc aggagctaaa gcaataatcg gttgtggaga atgattttta
    40501 tcccttacaa tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa gtcttcaata
    40561 gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa aaataggtgg
    40621 ccatgcttat ccataaaacc gtaatctccg ctatcccacc aatcgccata aacattttcc
    40681 tggaaccgtg catcttcctt gtaataagtc aaagcccgtc ctttagacag taattgtata
    40741 tgaccatctg tgttttcagg taggacattc ccaactgaat ctgtaatccg agcgcttgtc
    40801 atgtcttcca gtccaacgcc catatctctt gcatcacttg ttttcaaact ctctagtgta
    40861 tgagccttaa gaatcattgg tccgcattca ctttggccat atacttgaag aaataccgca
    40921 tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat agcatcaaaa
    40981 gtggagtgat agtatcggat acccgaaaaa gcttctggat gctccttagc agtaaaacgc
    41041 cactgaacaa agttattcgg atgggtctct agtgcaattg gccggtgagc ttccaacata
    41101 tgaacaactt ttgaactttg tgcgtttgct aaaggcatca taggaaatcc catggacatc
    41161 aaagaagaga taccaatatt gaatctggaa tgcactggag agatgtgaaa agcgattaat
    41221 ttcttttccg aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca tcccatagaa
    41281 tgagcagaat gacaaataag tttagggata ccagttgtac cagaagtatg ggtcatataa
    41341 gaaatttcat ttttgtctaa aaatgattga gaaacagaca aagcaggttg ttccattaaa
    41401 taaagaatag g
//
LOCUS       DACGLH010000016        41181 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.017, whole genome shotgun sequence.
ACCESSION   DACGLH010000016 DACGLH010000000
VERSION     DACGLH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.017"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            75..1073
                     /locus_tag="IXT21_000960"
     CDS             75..1073
                     /locus_tag="IXT21_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868384.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(1313..1396)
                     /locus_tag="IXT21_000961"
     tRNA            complement(1313..1396)
                     /locus_tag="IXT21_000961"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1360..1362),aa:Leu,seq:gag)
     gene            1779..2996
                     /locus_tag="IXT21_000962"
     CDS             1779..2996
                     /locus_tag="IXT21_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4868385.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3180..4016
                     /locus_tag="IXT21_000963"
     CDS             3180..4016
                     /locus_tag="IXT21_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4868386.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4092..5240
                     /gene="nagA"
                     /locus_tag="IXT21_000964"
     CDS             4092..5240
                     /gene="nagA"
                     /locus_tag="IXT21_000964"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ4868387.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5461..6519
                     /locus_tag="IXT21_000965"
     CDS             5461..6519
                     /locus_tag="IXT21_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ4868388.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6538..7557
                     /locus_tag="IXT21_000966"
     CDS             6538..7557
                     /locus_tag="IXT21_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ4868389.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7659..8375)
                     /locus_tag="IXT21_000967"
     CDS             complement(7659..8375)
                     /locus_tag="IXT21_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ4868390.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(8344..9129)
                     /locus_tag="IXT21_000968"
     CDS             complement(8344..9129)
                     /locus_tag="IXT21_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4868391.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9120..9869)
                     /locus_tag="IXT21_000969"
     CDS             complement(9120..9869)
                     /locus_tag="IXT21_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ4868392.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10167..10691
                     /locus_tag="IXT21_000970"
     CDS             10167..10691
                     /locus_tag="IXT21_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868393.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10707..12206
                     /locus_tag="IXT21_000971"
     CDS             10707..12206
                     /locus_tag="IXT21_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ4868394.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12294..12896)
                     /locus_tag="IXT21_000972"
     CDS             complement(12294..12896)
                     /locus_tag="IXT21_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4868395.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            13000..13956
                     /locus_tag="IXT21_000973"
     CDS             13000..13956
                     /locus_tag="IXT21_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ4868396.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14261..15217)
                     /locus_tag="IXT21_000974"
     CDS             complement(14261..15217)
                     /locus_tag="IXT21_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4868397.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15391..15951
                     /locus_tag="IXT21_000975"
     CDS             15391..15951
                     /locus_tag="IXT21_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868398.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15944..16768
                     /locus_tag="IXT21_000976"
     CDS             15944..16768
                     /locus_tag="IXT21_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ4868399.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16778..17395
                     /locus_tag="IXT21_000977"
     CDS             16778..17395
                     /locus_tag="IXT21_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ4868400.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17413..18096
                     /locus_tag="IXT21_000978"
     CDS             17413..18096
                     /locus_tag="IXT21_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ4868401.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18778..20061
                     /locus_tag="IXT21_000979"
     CDS             18778..20061
                     /locus_tag="IXT21_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4868402.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            20077..20964
                     /locus_tag="IXT21_000980"
     CDS             20077..20964
                     /locus_tag="IXT21_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4868403.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20981..21817
                     /locus_tag="IXT21_000981"
     CDS             20981..21817
                     /locus_tag="IXT21_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4868404.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21820..22443
                     /locus_tag="IXT21_000982"
     CDS             21820..22443
                     /locus_tag="IXT21_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868405.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22440..23732
                     /locus_tag="IXT21_000983"
     CDS             22440..23732
                     /locus_tag="IXT21_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4868406.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24091..24188
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24304..24885
                     /locus_tag="IXT21_000984"
     CDS             24304..24885
                     /locus_tag="IXT21_000984"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ4868407.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24888..26195
                     /locus_tag="IXT21_000985"
     CDS             24888..26195
                     /locus_tag="IXT21_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ4868408.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26611..27099
                     /gene="purE"
                     /locus_tag="IXT21_000986"
     CDS             26611..27099
                     /gene="purE"
                     /locus_tag="IXT21_000986"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ4868409.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27083..28216
                     /gene="purK"
                     /locus_tag="IXT21_000987"
     CDS             27083..28216
                     /gene="purK"
                     /locus_tag="IXT21_000987"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ4868410.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28229..29524
                     /gene="purB"
                     /locus_tag="IXT21_000988"
     CDS             28229..29524
                     /gene="purB"
                     /locus_tag="IXT21_000988"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ4868411.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29704..30081)
                     /locus_tag="IXT21_000989"
     CDS             complement(29704..30081)
                     /locus_tag="IXT21_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868412.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30337..31065
                     /locus_tag="IXT21_000990"
     CDS             30337..31065
                     /locus_tag="IXT21_000990"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ4868413.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            31065..31319
                     /gene="purS"
                     /locus_tag="IXT21_000991"
     CDS             31065..31319
                     /gene="purS"
                     /locus_tag="IXT21_000991"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ4868414.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31321..31992
                     /gene="purQ"
                     /locus_tag="IXT21_000992"
     CDS             31321..31992
                     /gene="purQ"
                     /locus_tag="IXT21_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ4868415.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31993..34215
                     /gene="purL"
                     /locus_tag="IXT21_000993"
     CDS             31993..34215
                     /gene="purL"
                     /locus_tag="IXT21_000993"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ4868416.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            34200..35639
                     /locus_tag="IXT21_000994"
     CDS             34200..35639
                     /locus_tag="IXT21_000994"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ4868417.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35671..36714
                     /locus_tag="IXT21_000995"
     CDS             35671..36714
                     /locus_tag="IXT21_000995"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ4868418.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36711..37289
                     /gene="purN"
                     /locus_tag="IXT21_000996"
     CDS             36711..37289
                     /gene="purN"
                     /locus_tag="IXT21_000996"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ4868419.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37286..38821
                     /gene="purH"
                     /locus_tag="IXT21_000997"
     CDS             37286..38821
                     /gene="purH"
                     /locus_tag="IXT21_000997"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ4868420.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38837..40111
                     /gene="purD"
                     /locus_tag="IXT21_000998"
     CDS             38837..40111
                     /gene="purD"
                     /locus_tag="IXT21_000998"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ4868421.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            40233..41147
                     /locus_tag="IXT21_000999"
     CDS             40233..41147
                     /locus_tag="IXT21_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ4868422.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT        13177 a         7027 c         9029 g        11948 t
ORIGIN      
        1 atgtttttga gaaaataaat gttaaaaatt tattattggt ttgctctata tactgtttaa
       61 taccattgac agtaatgggg gaaaccggtt ggagagcaac caccttgaac tatcagtggc
      121 cagtagcatt tagtttacta actttttatc cgttttttca actcttaaga ggagaagaaa
      181 taaatagaaa aatatattgg gtaagtattc ctttattgat atttttaact aatcaagaac
      241 aagtcaacgc ctgttttttt gttctaacaa gtatagttag tttatatttg attgtcaatg
      301 gacgatataa ttataagtta tcagtatttt caataataag cttagcagaa ttaattttct
      361 ctttgacaac tccagggaat gcattacgtg ctgcacatga aattaataaa tggtttcctg
      421 aatataaaaa tttcaacttt cttaataagt tagatttagg tatttcatca tttggaaagc
      481 cgtttttttt agacatgaat atcttatttt tattgctatt tttcttaatt ttccttctaa
      541 catataggaa atgtcaaaac tattatgtgc gtatattgac tgcattaccg tttttcttaa
      601 acctgattat ttattttggt aatacaatgg gtcaaagctt tacttacgtc aatggaaata
      661 aaagggcaat gatttggagt agtagtaacc tgaataatct ctttacagag ttaggaacga
      721 aactgtcact tttttatcca ggtacttgga tagctacttt agtagtcttg gcattattac
      781 tatgtttaat tgttggaatt tatttaagtt ttgacaataa gaaaacttct atttttttag
      841 ttattttgat gataatggga ttttgttcaa ggttaattat gggattttct cccacagtat
      901 gggcttccgg aatgcggacg tactatatac tttatgttgt tatcgccatc ttagtactaa
      961 tggcagttaa agagctaatg aaaagcatga gtgtacaaaa aaatgaattt atgcaatttg
     1021 gattaaccgt gcttggtatt tgtaccttta taataacggt gataaacaga taatatacaa
     1081 aaaatccctc gctttagtat taggtgaggg attttactcg tttttcgatt aattgtttta
     1141 acacttgcaa gtttctacga gtagcttgtt tccgaccttc atatactcac ctcaggctgt
     1201 ttacatgatt gtacgttctt tttgtaaagg atacagtgat atgtatataa tggcataaag
     1261 aaacgttcaa acaaaaaaac tcgtgaagca aaattaactt cacgagttgt agtgcagatg
     1321 atgggactcg aacccacacg agctaacgct cacatgaccc tcaatcatgc ttgtctgcca
     1381 attccaacac atctgcgtaa taataacaca ctaatgatag cgaggtttta catagttgtc
     1441 aagctcaaaa atgggaggac ttattaagat ggagaaatat gacggagaat tttcaggact
     1501 gggaatgatc ttaggtgtat tgattggact tgcattcgga cgttttttgc ttggttttat
     1561 gctaggaatc atttgtggaa ttgcaatgga ttgggcagcg aatctatgga atgactatca
     1621 tgacaattaa gttctcaacg cttgtttgtg tttttttaat acttacgttt ttcctaaata
     1681 agtggcaaaa ttttctagga tggagtatga tattgaaata gtaaataaca aaaaatttgg
     1741 aaagttctaa ctttttgaaa tgagggattg gctaagtgat gagagaattt gaaaaatcca
     1801 acaaattaga aggtgtcagt tacgatgttc gcggtcccgt tctagaagaa gcggagcgga
     1861 tgcaagaaga gggcattagt attttaaaat tgaacacggg aaatcccgca ccttttggat
     1921 ttgaagcacc aaacgaagtc attcgtgatt tgatcatgaa tgcccgtgac tcagaaggct
     1981 actcagattc caaggggatc ttttctgcta gaaaagcaat tgagcaatat tgccaactga
     2041 aacattttcc gaatgtgacg atcaatgata tctacaccgg caacggagtg agcgaattga
     2101 tcacgatgtg tatgcagggg ttgctggaca atggagatga agtactggtt ccaatgccgg
     2161 actatccgtt gtggacggct tctattgcat tagctggagg cacacctgtt cattatattt
     2221 gcgatgaaga ggcagaatgg tatcctgata ttgatgatat caaatcaaaa ataacgagtc
     2281 ggacaaaagc tattgttatc attaatccta ataatcctac tggggcactt tatccaaaag
     2341 aattattaga agaaatcgtt gaagtagctc gacaaaataa cctgattatt tattccgacg
     2401 aaatctatga tcgcttagtg atggatggtc tagaacatat tccgattgct acattagcgc
     2461 ctgatttgtt cgttgttacg ctgaacggtc tatccaaatc tcatcgtgta gcaggatttc
     2521 gagtaggctg gatggtccta agtggagata agtcacacgt caaaggctat atagaagggc
     2581 tgaatatgtt gtcctccatg cgtttgtgtt ccaatgtatt gtctcagcaa attatccaaa
     2641 ccgcgctagg cggttatcaa agtgtcgatg agctgctcct gccaggcgga aggatctatg
     2701 aacaaagaga atatatctat aatgcaatca atgatattcc tggtctttca gccgtaaaac
     2761 caaaagctgc tttttatata ttcccaaaaa ttgatacaaa gcgattcaat attttggatg
     2821 atgaaaaatt cgttttggac tttttacatg aacatcatat tctacttgta catggtggtg
     2881 gatttaattg gaaccagcca gatcattttc gaatcgttta tcttcctaaa atggatgatt
     2941 tgaaacaaac agctaagaaa atgagagaat tcctagcaac ctatcgccaa aaatgatttt
     3001 aaaaaccaaa tttttcagta ggagggccac agacaaacac actgaggctc tctttctagt
     3061 ggagttttct taaataacta aagtatatta tttagttgca agtcttttct tgaagtttgc
     3121 gacttcctaa agtaaaatac gaatagaatg cgaatacgca tattttgggg aggatactta
     3181 tggaaacgtt aatcgagtcc tttggaacga tcattaaaga gattcgaaaa gaacaaaaaa
     3241 tgacgcaaaa aatgctttcg catgatattt gttcacaaag tgtattaagt cggatcgaaa
     3301 ataatgagga actgccgaat gtattggtga tgatgcagat ttgtcaacgg ctaggggtaa
     3361 cgattgatca tgtgatgaat ctctctcgaa aaactactcg gacaaacgat aaattatttg
     3421 aactgttgga tagttgtttc caaaaacgtg attatcggaa gttagaacaa gtactaaaat
     3481 cttctgatat ttctgaaaat ctgtatcaag caactgactt tcaacgctat tattattatc
     3541 tcggtgtttg cgaattcacg ttgcgtcaaa atatctctca gtccctttat tatttgaaag
     3601 aagcattaag ctactctagc caaaaagaaa gaatccatgt ttctgacaca gaaatacaac
     3661 tgattagttg tatcggaaaa atatatggtg tggcaggaaa aacggctgaa gcacgctact
     3721 atttgagtcg aagcatccaa ctgctgcatc aatcagctga tgaacgatca aaaagtgaat
     3781 tttcgcaaat tttttataat tatggaaatt tcttattcca tcaaaatgaa atcgacgaag
     3841 cgttagaaca ggtgaatcaa ggaatctatt gggctcaaga gaagaatagc tactattacc
     3901 tcaatgattt atttgtgctt aaaagcttaa tatacaagcg taaagaagaa ccagaaagag
     3961 cagcattcta tgaaggctta gcacaagctg tgaaacaaat ttcaaaaaat ttgtaaatgg
     4021 tgtagaccat tcttgacttc ttctctaaat aagagtactt tagaggtaga aaaacaaagg
     4081 gagagaatag aatgagaact tttatctatg ctgacaaatt ctttttagcg tcaggtgtca
     4141 aacatgcagg ctatcttgaa attaccgatg gactttttgg tgaatacacc aaagaactac
     4201 ctgagggaga agtcaacatc attgaccaaa gcggcaaatg gattgctcct ggtttggtag
     4261 atacacatat tcacggctat atgaatcatg atgtaatgga taatgatgca gaaggcatca
     4321 aagtgatgtc tgaaggtctg ttatcttgtg gagtgacttc atttttacca acaacattga
     4381 cgtcaagcaa agaacgttta aaagatgtag ctgagacaat cggcaaaatg tatcaggaag
     4441 caccaggagc taaaatcaaa ggaatctatt ttgaaggtcc tttcttcaca gaagaacaca
     4501 aaggggcaca aaacccaagt tatttcgggg atcctgattt ggatactttc catgaatggc
     4561 aagaagcttc aggtggttta atcaaaaaaa ttgctttagc accggaacgt caaggtgtag
     4621 aagagtttgt caaacaagtg actgaagaag gagtagttgt ttctctagga cacagtaatg
     4681 ctacactaga agaagcgcaa aaagctgttg aagcaggcgc aagtgtattt gtccatgctt
     4741 tcaatggtat gcgtggattg aatcatcgcg aaccaggtat ggtgggtgcg ttattatctt
     4801 tagaacatgt cttttctgaa ttgatttgtg atggccatca tgtacatcca aatgctgcgg
     4861 gaatcctaat ggaaaaagca ggacatgatc atgtcgcatt aattacagac tgtatgatgg
     4921 ccggtgggat gcctgatgga aattacaatt taggcgaatt tccagtagta gtaaaagaag
     4981 gaacagcacg tctagaatct ggcaacttag ctggaagtat attgaaattg aaagaagcaa
     5041 ttaaaaatgt tgtagactgg ggattagcaa caccagaaca agcagtcatg atggctagtc
     5101 tgattccagc aatcagctgt aaaatcgatg atacatgtgg aaaaatcgct caaggacgcg
     5161 atgcagactt tatcgtattg aaccctgata tgacattgga agcaacttat ctagatggcg
     5221 tggaacgttt ccacgcataa tataatttaa gttaacctta aaactaaact tcttaagttt
     5281 ggttttaagg ttttttttgt gttctagtaa aaatcacttt acataagttt caaaagtttc
     5341 tttaatttcc tattgttcaa taagtaacaa tgtaaagata aaattaaaaa tgttaatacg
     5401 tattagcaaa ataaaagctt tttttattgt gaataaatga acataattag gaggagaaag
     5461 atgaacttat ttgacatttt tcgatcgact ttgacagata tgtcgattgt cagtgcaatc
     5521 acttcaacag tttttattat attattaggg tttgtttgta gaaaaaaggg gattttttct
     5581 gaatctgttg gaaaaattct atcgaaagtc gtcttaacag tggctttgcc agcactggca
     5641 tttcgagctt tcatgcaaga tatcaataca gaattactga tgcaaggaat caatgtcttg
     5701 atttggggat tagttattta tattattctt attctagttt caaaaccttt tttcttaagc
     5761 tataaagggg ataagcaaac aacgttgcgt gtcctgacga tctttggttc tacgaccttt
     5821 ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta ttcctcaatt
     5881 ttcaatattg gctaccgaat ctttttgtat tcttatggat acataaaaat gagtggcttg
     5941 aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc caatcattat cgctactttt
     6001 gccggccttt tcatttggct gttccaaggg tatctgccac aagtgtcagt tacaggtgca
     6061 gaaggagaga taagccaagt agcgtttttg cgaattgacc agactgcacc gtggctattt
     6121 cagccgatgg attatttagc tggtttaagt tcaccattgg cgtggctagc aatcggcgca
     6181 acattaggtg aagtaagctt caaaaaagct gcatctgaaa aaacttcatg gtattacagt
     6241 ctagtcaaag tggtatttgt acctgcattg aatatcgtga ttctagcagt tttgacggca
     6301 acgaatattc taccagtcaa ttttgaagca ctggcaacaa tcgttatcat gatggctaca
     6361 ccagctgcaa cagtggcagc agcctatgct atcagttttg atagggaggc acttttagcc
     6421 tccaatgcat ccttactgtc aactattttt gctgtggtac tgacacctgt atggatcgtt
     6481 gtattacaaa tgattcatgg cataggttta ttttcataag aaatggagga agaaagtatg
     6541 agaatcgcat gttacggtgt tcgtccgaac gagatagatt tttttgaaaa attgaatatt
     6601 taccattatg atctcagtct ttatgaagaa ctgttgacac atgacaatat cgtgtcagca
     6661 aaggaccatg atgctgtatt gttgagggga aattgtatag cagatgagac caatcttgca
     6721 aaaatgcagg aatatgggat tcgttatgtt tttacacgga ctgtgggagt caatcatatc
     6781 gatctacaag cggcagctga ttttggtatg acagtagccc gtgtaccatc gtattcgcca
     6841 aatgcgatag cagagttatc tttgactttt gccatgatgc ttttgagaaa tactgcgtat
     6901 acgacgatcc gaacatcttt caaagatttt cgagtggatg agcaaatgtt tagtcgtgaa
     6961 atcagaaatt gcacagtagg gatcatagga acaggcagga tagggttgac ggaagctgtt
     7021 ttgtttaagg gactaggagc caaagtgatc ggctacgata ttcatcagac agaagctgct
     7081 aaaaaagtat tgacgttcca ctcgcttgaa gagttattag ccaaaagcga tattgtcagt
     7141 attcatgtac ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg atttttagct
     7201 caaatgaaaa aaggtagcat cttgattaat acagcccgtg gagagcttca agataatgaa
     7261 gctattttaa gagctttgaa aagcaatcat ttagctggtt ttgcaaccga tgtttttgca
     7321 aatgaaacag aggttttttt ccgatccttc aagccatggg agacgattcc agaccctgct
     7381 atccagcaat tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt aggttcaaat
     7441 acagatgagg cattatcaaa tatgatttcg acaagtttcg agaactttcg tgaaataata
     7501 gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt aagataagaa
     7561 aacatggata ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt tgttacttct
     7621 gtatacttgg actattttgt atgggataat tcgatgactc aacagtaatc tgtgagcttt
     7681 ttccgcccat tctttgtcaa ttcataacag gttagcggcc ggccgatttc caaatatttg
     7741 attctttcgt tcaatatatc ttgttctact aaaaaattca aatatttctt gacggatata
     7801 cgtgacagtg agcttttttg agccagttct tttgttgaaa aaatagtatc ttcttttaga
     7861 ataaatgcac ttattttttt taaagtcagt tttgacagac ctttaggcaa atcgatgaat
     7921 gatggtattt cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa tttctgatcg
     7981 gctttttcag tcgtttggat tgtctgagag aaagcaagat acttttcgat cgccagctga
     8041 aaacgttgca aagtgaacgg tttgatcaaa taatctaagg caccgtagcg gatcacattc
     8101 tttatggttt cgatatcatc tgcagcagtg atcaaaatca ctgggatatc atttccttgc
     8161 tgtctaagcc aagataaaaa atggatacca ctttcgccag gcatgtatac atcaagcagc
     8221 agcagatctg cgtttttttg tctgaaatat ttttttcctt cttctacatt tctgaaactt
     8281 tcgattacgt tcatattagg gatgctttcc acaaaagtac gattcaattc tgcaaccatt
     8341 ggatcatctt cgattatcag gacatttgtc atcattttga tcttcctttt ttggtaattc
     8401 tagatagacg attgtaccgc catttgtttg tttggaaaat tctaaaaatc caccgtggcg
     8461 tttgacgatt gaatggatga gattcaatcc aaacccacga ttgattccct tcgtcgaaaa
     8521 acctagcgta aatatttttt ctgagatttc ttctgatatc ccgacaccgt catcgcagac
     8581 ggtcacgatg aatacttcag cttctgtgtc atagtttaaa tgaatccgga cctgtccgtt
     8641 tctttcatgt ttttgtagca cggcttcttt tgcattatca agtaagttcc ctaacacttg
     8701 caaaagtgaa tggatggagt catccatctc cattttaggg ataaaagaat cgctttctaa
     8761 cagcatttca acagattgtt ctttggcttc gtttattttg cctaataaaa aaccggcaat
     8821 tgcaggtact ttgatcagac ttgagacttg gccaatctct tttttataat tttgatttag
     8881 ctgctggata aaatgggtga cttcttcata cttcttcaaa tcaatcaaac cagaaatgac
     8941 atgaagttta ttcatgaatt catgggtttg agctctcatg gagtttatat attgtttcgt
     9001 ccccccaagc tcctccatta atttcattac ttctgatcga tttcgtaaag ttgcgactgc
     9061 tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta gaaatagttt ctatcccatt
     9121 taaatacatg gattgagtcc gaacggtcgt ttgatgctca atcgtctctt taaacaaaac
     9181 gtcatataga ttatcactga tttttttcct acttcgaaat ccaatagctg ctctgtttgg
     9241 tagaaaatgt tttttgcagg tgtattgatc aagaggatag ttttatcaga agagatggct
     9301 aagattcctt catctacttc attttcgata atcgtttttt cacttaactg ttctgctatt
     9361 tcttgtggtt caagtccaag cagtactttt ttcatttttt gggcaagaaa aacggcgcct
     9421 atgatgccta cgctaagtcc aataatcaat gaaaaaacaa tcattttttg tccatctttg
     9481 acttgattgt caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat aatctcttga
     9541 tcataataaa tagggacgaa atagcggaag ccgaacccta acattccttc tttttcagag
     9601 aaaaattctt gtcctccaat cacttttttc acatcttcat gacttgagaa aggaagaccg
     9661 atgtaacttg ggtctgcgtg agaatatcga atgccttcag ggtccatgac aacgataaaa
     9721 tctaaattcg atgcaatcat gatttcattt gttattcctt gaatcgcatg tgtcgggtca
     9781 ccagtctgca gctttttttg gatgagttta tcattggcaa cgattttagc aatgctagag
     9841 attttatctg acacactaga tcggttcatc gaaagaataa aattcctgat caatatggca
     9901 gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt gttctggagc
     9961 tttaatggtt ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat ttattgtgaa
    10021 aattttaaca tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa ccttcctata
    10081 aaaaacacca ctgagtcggt aaaagaagct atacagaatt ttcaaacatt ggtatgatag
    10141 acgtagaaat ggaaggaggc gtgtatatga tagggggaat cgcacttgag acgatttatt
    10201 ggtatacatt gctgatttgt gcaggattgg cagtcctatt gattattttt ggagatgtat
    10261 tcgattttga tggcccgctt gatccgatgc ttattatccc ttggttaact ttcacaagct
    10321 tatttggttt tctaggtgag cgtttaacag atcaatcatc tttattttta tttattgcta
    10381 gtggaggcat atcgatagtg ttggtatttt tgcttaattt ctatatattg atgccgatga
    10441 aacatgccga atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa gtagctgttg
    10501 tgatcacacc gatccctgta cgaggtatgg gagaaattca gttgaaaagt gttaccggtt
    10561 ccattagtcg tccagcttgt ttttacagcc cccaagaaca gacagctgag cggggaacga
    10621 aagtattgat cattgagatc aaggaaagaa tctgttatgt catgccttat gaaagcatga
    10681 taaaagctta aaggagcgtg tgttaaatgg aaataccagt tgtttttatc gcgatcgcaa
    10741 tcgcagcttt gattttactg atgttgctga tcgtctttgt agcgaagtac cagacagcaa
    10801 aaccggatga agcgttgatc atcagtggaa gttatttagg aagcaaaaat gtgcacattg
    10861 atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc ttgccagttt
    10921 ttcaacgttc gaatcggatc agtctattat cgagtaaatt ggacgtctcc acccctgaag
    10981 tgtatacgga acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc aaaatcggtt
    11041 catctgtaga agagattgct acagcagcag aacagttttt aggcaagaca agagaagaac
    11101 tggaaaatga agcaagagaa gtattagaag gacacttgcg ctcgattctt ggttctatga
    11161 ctgtagaaga aatctatcaa aatcgcgata agttcagtca aagcgtacaa gaagtcgctt
    11221 ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa gaggtcagag
    11281 acaaaaacgg ttatcttgac tctttaggga aaccaagaat cgcccaagtc aaaagagatg
    11341 cagatatagc agaagcggaa gctctaaaag aaacgcgtat caaaaaagct caagcagaaa
    11401 aagaatcaca agcagctgaa ttacagcgac aaacagagat cgcagaatca ttaaaagaaa
    11461 aagaactgaa attagcaacc tacaaacaag aacaagatgt tgctaaagca aaagcagacc
    11521 aagcctacaa tttagaaagt gcccgagcac aacaacaagt aatcgaacag gaaatgcaga
    11581 ttaaaatcgt agaacgtcaa aaacaaatcg aactggaaga aaaagaaatc acacgtcgtg
    11641 aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac gctaaagaac
    11701 aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa cgattccgtg
    11761 tggaagcact ggcagaagcg gaagccaata agacacgttt agctggacaa gcagaagccg
    11821 aagcagcact agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc gccaatgcct
    11881 tcaaggaata tggcgaagca gctgtcttga gtatggttat cgatatgcta ccgcaattga
    11941 tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc gatacaggaa
    12001 gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca aatttgcttt
    12061 cttctactca agaaacacta aaagcgacaa caggacttga cttgaaggaa atcgtagaga
    12121 atttgtcgac acgtgggact actagccaag taaatatgta tgaagcgcca aaagaggcag
    12181 aacaaaactc gatgaaggaa gaataaagta gtacacttca ccgttcaatc aaaaatggag
    12241 gaagactttt taaagttctt ctctccattt ttttatttta ttgcaaaaga aaattagtcc
    12301 tcgctatttt tcttttgttt ttggatctta gcttctatct cttgtaaatt tgttcttagc
    12361 aatcgttctt tcttttggag ttcgaccaaa tcttctgata gttcttcctt cttttctgtt
    12421 tcaggaggac gaagaaagaa attgataccg attgcaatga atgtccctat aaaagtatct
    12481 agtacacgtt ggatagcata caaagcggat tcgccttgag ggatgctcaa cgcaatcagc
    12541 aacaacgtag ctattgcgga aatgataccg gaattattgt taataccatc tgacacgacg
    12601 atcacaataa taactagaat aggaagcaag atcaattcta ccaaaaaatc attgtgaaaa
    12661 tagcttttga caaaaaaata aatgatagct aatgtaccac ctaatgtatt gccgatgatc
    12721 ctagactttc caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa aacagcagat
    12781 aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat acataaaaaa
    12841 acagctaagg cacttttgat cgtacgcata cctagtcgaa aacggccaaa ttgcatagag
    12901 attctccttt ttggtctttt tactatcata cctaaacttt tgtctgatag ctagtttttc
    12961 attattcaag tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg aatgcgaaca
    13021 gtcaaaacag tgatcagtgt cttcttttcg atggtcacag cgggtagctt atcgctgttg
    13081 tattggccag ctgcagggat cattgcatta ttaagtgtag gaaataccaa gcgctctaca
    13141 ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag ctacggcaac agcactgttc
    13201 agtttttttg ctgttgggta tactattttt ggcttcagtc tttttttatt cctgttcatc
    13261 ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag taaattcggt tttggtcact
    13321 cattatttag tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc gggattaatg
    13381 gcgattggat tagtatttgc tttgcttgca aatgtttaca tgccagatcg tacgaagcag
    13441 ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa atattttgag acaaatggca
    13501 gaatttcttt tatcagaaaa taaagaagat gtgcagatca aatgcgaaca tttactgaca
    13561 ttcattcgcg aaagccaaga agatgccaga gaacatcaag aaaactactg gctcagacag
    13621 ccgctatact acgaaactta cttctctatg agaagagcac aggccaatgt gattaaagac
    13681 atgctggaaa atctagagag aatccagcaa ccagcttatt atggaaaaca tatctatggt
    13741 ttattgattt atactgcaga aacattttca gagtcaaatg atggcagaca aattttaaca
    13801 cgtattgaag aagtttatgt attgtatcgg caaatgcctc tgccgactag tcgccctgaa
    13861 ttcgaggatc gagcagaatt gttccagttt ttgcagtctt ttaaaagttt tattgaaatc
    13921 aaggcagaat tctcccaaca aaaaatccaa aaatagaggt tgtgaattca gctaatattc
    13981 aggtcaaaaa gctgatttca caacctcttc tttaaacggt gttctcctgt caactcctcg
    14041 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    14101 ctcggagctg aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa agctttcctc
    14161 tgcggtaata agtccagcca aacgaaaaat aggaggagct atttttgttt ggctgttctt
    14221 attacttgat gcagaacagc tttttcacaa ccttttaaaa ttacatattt ttccatagaa
    14281 cttgcatttg atccaagatt tcgatgacat cttttgtcaa gaagagagag cgattgggca
    14341 agtcaccttc aatcatgtgt accatattct ttatttcgta attcattgct tcagatgtat
    14401 tcccgctttc gatgaattcc tttgtaccat ctgtaaataa gatctccgca cgatcagcac
    14461 gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc aacgataccg acttttggca
    14521 tttttgcctg aaaagtcaag ctgacagcag caagttcatt ttctttgtta cgcaagatag
    14581 tgacggattg ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca actacttcag
    14641 gttgagagct caaaaagaaa cgagcgaaag atacagcata cgtaccgata tccaacagag
    14701 ctcctccagc taattctgga ttgaagaacc ggtttttagg gtctggttct ttgtaactgc
    14761 cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc tgtatccatt attgagcgta
    14821 actgctgata aagtggcatg ttgaagattg tcatcgcttc tgctaagatc aaattctttt
    14881 cttctgctaa gcgcatagcg tcagccagtt cttttttatt catcgtgatg gctttttcac
    14941 atagaacgtg tttgcctgct tccaatgctg ccaagatatg gtcgatatgc tgtctatttg
    15001 gtacagcaat gtacacgatg tcgatttcag catcgtctaa catttcttga tagcttccgt
    15061 aagcttttgg aatattgtaa cggtgagcga agttttccgc tttttctgaa gttctggaag
    15121 caactgctgt tagttcgctc gtctcttgat tgaagctttc ggcaaactca tgtgcgatgg
    15181 taccaagacc aacgattccc cattgatatt ttttcatctg aaaacgctcc ttcatagttg
    15241 gattgagttt cttgtctgtc tattattata acaaaaatca cgaagaataa agaggagagg
    15301 acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg atcaaaactt
    15361 aagtagaata gttaaaagga ggggataaac gtgcatcaag tatatattga tcttattatg
    15421 agtgtattat tagaacagtt tgaaactgaa aaaaattttt tagatgaata cttgctgatc
    15481 agtccagaac aatggcaaaa atggaaagat ggcgagaaaa atctgccatc tgaagcgatg
    15541 cagaaagtaa aaaatctttt cactgattat gaatggatgc taacgcaaaa gattttgcgt
    15601 caaacgatta tttttcccga aaaacgaaac gttgccgttg cagagtacaa gcaaatcaaa
    15661 acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct gatccctttg
    15721 aaaaataatc agcaggctgg tagatacctt gacttgaaag taagtattca atatgatgaa
    15781 tggggctatg atgatatttt gaattttcgt ctgccagcag atgttcaaaa acagatcgaa
    15841 ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc tggaagaaac ttacgttcat
    15901 gaggcgaatg gggaacgtga agaacaacga aaggattttt caagtgaata aatttttagg
    15961 atttggagca gttgtgatta tgatcgcagc aatgggctat gttattttta gcgatgatga
    16021 ttcgcatgtt caagccggca atcaagcagt cgaacgaaca agcaccaaaa caactgctag
    16081 taaagaaaag aacagtcaaa caaccagcag caaggtaaag gacaaagaaa caaaagatcg
    16141 tacgtccaaa caattgccag atgttaaaac agacgattgg aagctagttt tggttggtcc
    16201 agatcacaaa ttagaaaaag aaattgatga gtctactcaa ttggcatcat taagtaacgg
    16261 atacttgatc gacaaacgca ttgctccaga atatgaagca tttgccaagg ctgctgaagc
    16321 agctggattt ccattagtga tggtctcagc ttaccggtct gtatcatccc aacaacaagt
    16381 tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag aggaggcgac
    16441 aaagattact aaagaaacga ttacggagcc aggatacagc gaacaccaca caggtctggc
    16501 agtcgatgtc gtcgatcaaa actggtacaa cagttatcct tctactgtat tggatgcaag
    16561 ttacggagat cagcctggag ctaaatggat cgcagataat gctgcgcagt atggatttat
    16621 cgttcgttat ccaaaaggac acgaagatat aactaagatc acatatgaac cttggcactt
    16681 acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa caccaattga cgctagaaga
    16741 atatctgaat gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa agtggaaaaa
    16801 gcaaaagcgc aagaatatcc gttggccagc agtggttgcc ggtgtagcag tgatactctt
    16861 agcctttgtt ttttccttga agaatctgtc ttctcctttt actgataatc agcaagaaaa
    16921 tgaagcgcaa tcccatcagc aatttattga acggcttgtt ccgcatgccc aagaactaca
    16981 agatggttat ggagtgcttc ctagtatcat tttaggacaa gctatattag aatcaaattg
    17041 gggaaaaagt accttggcta gcaaatacaa taatcttttc gggatcaagg cttatgggga
    17101 tcagaagaaa gtaagcctgg aaacaaagga atttgttaac gaagaatgga taactatcca
    17161 aggagacttc aaagtgtatg attcatggga acagtccatg gatgaccata cacaactgtt
    17221 cgtccaagga gtagattgga atcctgcgct gtatgaaaaa gtgattacgg ccaccaacta
    17281 tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg acagaccccg gttatgcgca
    17341 aaaaatcatc caggtcatcg agacgtatca attgaatcag tatgaccgga gatagcaaag
    17401 gagaagtggc tgatggcaga acaatttacg ccagaaatca tgacggaatt gagaaaagat
    17461 atcgtaagag taccggaaat cattttagaa gcaagcggga tcaagatatt tggaaaattg
    17521 atccgttcga tcatctttac tacggatatt gcgatcatac gtaatacaaa tgcgaatgca
    17581 gtaattgctg tgtatccttt tacacctcat ccagcgatta ctaaaagcat tattgaggca
    17641 gctgacattc cagttttctc cggtgtaggt ggtggtttga cacaaggtgc tcgttcttcc
    17701 tacatgagca tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa cggtcctacg
    17761 cctttagaga cagtaaaaga agtttgtcga gtagttgata ttccagttat cagtacggta
    17821 acgtcgaaat atacagaaat agatgaaaag ttagcagctg gtatttcgat tatcaatatc
    17881 agtgcaggta aagaaactgc agaagcagta cgccattttc gtaaaaaata tcctgaactt
    17941 cctatcattg cgacaggtgg tccaacggaa gaaagtattg ctgagaccat tcaagcagga
    18001 gcaaatgcca tcacatatac gccaccaagt agcggagagc tttttagtcg taaaatggaa
    18061 aaatatcggg aaactgagaa aaagaatcat gaataacagg tgagaatcaa aagacgagga
    18121 agagcagata gtggctcttc ctcgtctttt gtactccagt actatttgtt cagaattcaa
    18181 aaaaaagaga aatttttttg tttttttacc tgtaatgtac ataagaagag ttaaaacctt
    18241 ttgttattat tagcattcta acaaaaaatg tcttgattta ttgaagcgct ttctatagaa
    18301 tgaaaaacga ataaaaacta aaaaaggcac ccgacaattt agtagcagct aaattgtcgg
    18361 ggcttgaaaa gtcagcggga actgaccaat caagttgcca tagtcaatgc ttgtgcatcg
    18421 acgtgtatat tgtacctaat ttcatacaga attactagtt atttttctgt tttttaggga
    18481 agagcgcaca cgaagaagca tgcttattta gctatgtcaa ggatattggg cagttgtctg
    18541 atatcctttt ttctttgcgt aattatcctc ccatcattga agcaagaagt gtttttagcg
    18601 atgaaagaag aatcagcact atctaatttt tcttgaaagt attgagttgt tcatcctagg
    18661 cagaacgata aggcaagtct tggtttttat tccccttgaa aaagatcgtt taataaatat
    18721 gttctgtata aaaatacagt atattgaaaa ataacaatgg aagtagaggt gcagagaatg
    18781 aagaaaagaa tgagaatcat cttagcttta agtgttatgt tattcttctt gacggcttgt
    18841 gggcaagaag atgacaaaaa aatcacgctg aacatggctt ggtggggttc gcaagtccga
    18901 cacgatgcaa cagtaaaagt aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc
    18961 gattatgaat tttatgatta tgaaggatat tacacaaaat taggtacact agcagcatca
    19021 gatgatatct gggacatttt ccaactgaac aatcaatttc ctcaatacat caatcagatt
    19081 gaaccattag atgaatatgt ggagcaaggg ataattgata tttctaatgc agatgaatcc
    19141 tatttagcaa cgacgactta cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg
    19201 tttgcgattg cttacaataa aaatatattt gaacaattaa atatccctga accagcttca
    19261 aattggacat gggatgaatt tgaacaaatc agctatcaaa tcactgaaga attaggaatc
    19321 tatgctattt caagattcga agattttata gctggctgta ttattcaaat accacaagtt
    19381 gagaaaggat tgaattttta taatcgggaa gatctatcta acagcttggg atttgaaaat
    19441 ccagactatc taacagattt tttccagctg cgtaaaaatc tggtagacaa aggggcccat
    19501 ccagaaccag gtggtggagc tgcaagtacg acggatctgt cttttcaggc ggttagggat
    19561 agagaagcgg caatggtgtt tctatcaagt aaccagttga cagaaatatt gtcaggggct
    19621 cctgaagata tggagattca gttgattaat ccacctcgca gaaaagcaga tggagaatct
    19681 ggtatccctt tacgttcttc gcaaatgctg agtatggcaa aaagctcagc ccataaagaa
    19741 gaagctgcta agtttattga tttctttcaa aacagtgaag aagccaatga aattttaaaa
    19801 ggagaacgtg gtgtaccaat catgtcgaat attcgagaac agctcgctcg tgcagatgat
    19861 gcaactttgc aagcgacatt tgattattta gaaatgattt ccgatatgga caacggcgaa
    19921 gtgaatgtct ttgatagtgc tttgaatccg gaaattcaag accaatacaa tcttttagtt
    19981 gaaaaagtca tttatgaaga agtaacagca gaagaagcag cagagagtct ttatgagttt
    20041 gcgtcaaaca ttctaggata gcagcaggag gaaggcatga aaaaagaaaa actaaaacgg
    20101 ttcttggatc aagacaatgt ggtcggttac gtatttgctt ctccattcat cctaggattt
    20161 ctattgataa cagtgatacc gatgatcttg tcactttatt attcgttcac cgattatcgt
    20221 ttgggaaatc cagtctcatg gatcggcttg gataattata taaagatgct gagtgatgcc
    20281 cgttttttga actccatccg agtcactttt ctctatgtat ttgcatctgt acctgtgaaa
    20341 ctgatctttg gtctattgat tgcttatctt ttaacgagga atatcaaagg aacgactttc
    20401 tttcgttctt tgtattatgt gcctagtttg atcggtggca gtatttctat cgctctagtt
    20461 tggcgggagt tgttttctaa aaacgggtta gtgaatattt tcctggaacg attaggcgca
    20521 cctgcgcttt cctggtttag cgatcaaaaa ctggtcatga tccctcttgt tttgatgtct
    20581 gcttggcaat tcggttcggc aatgatcatc tttgctgcag gtatcaaaga gatcccaggc
    20641 tcgtattatg aagcggcaga gatcgatgga gcgaacaaat ggcagacgtt ttacaagatc
    20701 acgttacctc ttctttcgcc gatcattttg tataacttgg ttatgcagac aatcgcggcg
    20761 tttatgactt ttacgcaggc atttatcatt acccaaggtg gtccgaatga tgccacgaac
    20821 ttttatgctc tttatgtgta taaccaagct ttttctttta attctatggg gtatgctagt
    20881 gctatttcgt ggatcatgct gttgattatg ggagctgtca cctttctgat tttcaaatca
    20941 tcaaaaaaat gggtcgttgt gtgaatgaga ggaggaagaa atgaataata cagtaacgaa
    21001 atggtttcaa tacggtttta ttttgctatt aggtctagtc atgatttatc cggtcttatg
    21061 gatgatcgca ggttctttta aaacggatca agagattatg agtggttcgc tgaatttgat
    21121 tccagagact tttcaatggg aaaattacgc aaaaggatgg gcggggtttg ctggaatttc
    21181 attttttact ttctttagga attcgtttgt catctctgca gtttcgacga tagggacagt
    21241 tctgagctcg acttgtattg cttacgcatt atcgagaatc gatttcaaag gaaaaagatt
    21301 ctggttcgtc gtcatgttag taacgatgat gattcctgca caagtgattt tgattccgca
    21361 gtttattatt tacaatcggc tgaatttggt tgggacgtat gtgccgctga ttttaccaca
    21421 tttttttgga caagcatttt tcatttacca gatcatgcaa ttcatggtca gcatccccaa
    21481 agagttagat gaatcagcga ttattgacgg atgcagcaag tatagtgtct ttaccaaaat
    21541 tatttttcct ttgctgaaac cttcgatcat cacgacgatc atcatccagt tttattggaa
    21601 atgggacgac ttcatgggac cattgattta tttaaacaag ccgagatctt acacggtatc
    21661 gattgccatc aaattatttg cagacgctgg atctagtacg acttacgcga gtatgtttgc
    21721 gatgtctacg ttgtcgctgc taccggtttt tctgattttc ttattcttca ataaatatct
    21781 tgtccagggg atcagtacaa gcggtctgaa gggctgaata tgaaaaagcc aatagaagag
    21841 atgtttcttt acaagctcaa ctactatgtc tgtcggattt ttttaaccag tcttatcagt
    21901 aatgctcttt tcgctttagt tctaggatgt ttgttcactt taggattagt gctgtctgct
    21961 cgtgttgtct atctgattta ttttttgatg atgtatcctt cttattatct atctgtccat
    22021 tacttggcag aaaaaaaggt atgttggagg aaaggaaaaa tcaaacggat gtgtttcatc
    22081 attacgttgt ttcagctgtt tgtcattatt tttgcagttg attggtattt attagtggga
    22141 aagattggag cttggtatct gattcctttt ctattgtgtc tgttgctttt tgtatcccat
    22201 agttttttta gtacgctaat tgatatctgc aaagaagata aagcagatag tcttgtttat
    22261 ttgatcaaaa ggaatatctg gctgttgtca aaaaacgtca aggggtttct gtggtattta
    22321 tgccttttgt atggactgtt ctggtgtata cagcatgatt ttttactcac tgcattactg
    22381 tatccagggc tgccatttgt cacagtattt tgcttaaaag aaaaggagaa tgactttaaa
    22441 tgaagaaaaa atatgtacaa gtcggacttg gtgggagagc gcgttttttt tatgaggcaa
    22501 ttgcctctac gtatcatcaa acttgtgaac tagtcggttt ttgtgatgta tctcaaacaa
    22561 ggatggatta tgcaaatcgg atacttgtga acaagtatgg acagaaaaaa gtaaagacat
    22621 gtatgccatc acaatttgag caactactgg atgacgtacg gccggctgaa gtcattatta
    22681 cgtcagtaga tcgaacgcat catgattata tcattcgtgc aatgaaaaaa ggatatgatg
    22741 ttatttgcga aaaacctctg actatcgatg aggaaaaagc tcaagaaata ataacggtcc
    22801 aaaaacagac gggaagaagc ctgcgagtca cttttaatta tcgttatgca ccgtatcaca
    22861 cgaagataaa ggaactgata gaaagtgatg tgattggtga tgtgttttct atccacttcg
    22921 aatggctgtt aaatacgcaa cacggggcag attatttcag aagatggcat cgaaataagc
    22981 acaatagcgg cgggcttctt gttcataaat ctacccatca ttttgatcta gtgaattttt
    23041 ggttgaacag ccgtccccaa aaggtatttg cattcggtgg attaaacttt tatgggatgc
    23101 ataatgcgga gaaacgcgga gtagaaatat tttacagtcg atgctatgga agcgaactag
    23161 ctaaagctga tccatttgct atagacctgt cagccaatga ggaattgcga gaaatgtatt
    23221 tagaagcaga aaaagacagt ggatatattc gtgaccgcag tgtattttcc gatgatatct
    23281 ctattgaaga tacactaggc gtaacggtca aatatgaaac tggtgcgatt ttgacgtatt
    23341 cgctaaacgc gtatatgccg tgggaaggat tcaatgtttc tttcaatggc agcaaaggac
    23401 ggatcgaaat gaaagtgatc gaaaagtctt acatcaatgc aggtggagat aaaaaggatg
    23461 aaggagcagc cgttcttcag tccatccatg tctatccgat gttcggtgag ccatatgagg
    23521 taatcgtcga acaagaagcg ggcggacatg gcggaggaga tccgcgttta ttagatgatc
    23581 tatttggtga agaaaaagaa gaagatccgt ggaatcgggc agcaactcac gtagatggta
    23641 ttttgtcgat tttgacagga atcgcagcaa atcattctat tgcttcagga aaagcagtag
    23701 tgattgacga gcttgtcagc ttaaaagaat aagaatagat ttacggaaat tacttcccgt
    23761 agtttataaa atcttgcctt attgccgata ggctgtcttt tgaacactat ttattcggaa
    23821 aaaggggcta tgacatgctt tttgtcatgc cttcttttta tgtgggctgc ttttttagat
    23881 aggagaaaat aaaaataact atttaaaccc gaacaaaaag gaataatgca ttaaaaatca
    23941 ttagatttta caaaaaaata agtaaaccac gttgtcagtt tcagaaagta gtgttagact
    24001 gttttcaata tttgaaacaa cattttttat tcaaatatgg caaactgcct tcacgtgcaa
    24061 aacagttgca agaaaatgaa tttacactat tattagtttt acatacctac ttatatatcg
    24121 tcataatatg gatgacagtt tctagccagt accgtaaatg ctggactata agtaaaagat
    24181 tggctatttc acccttcttt tcttatgata aagtggaggg tgtttttctt taacagaaac
    24241 atagtaggtt tttgcacaaa agcggttatt tcggtattaa taaatgataa aggagcaaat
    24301 aaagtgaaag aattagttga acgtattcaa aaagacggac gtatattagg cgagggcgta
    24361 ttaaaagtcg acagcttcgt cactcaccaa gtagaccctg tattgatgga acaaatggga
    24421 aaacggtttg cagaagtatt taaagaacaa ggaatcacga aagttgtgac cattgaagct
    24481 tctgggatcg caccagcttt atttgctgca agagaattag atgttccaat gatcttcgct
    24541 agaaaagcaa aaagtttgac gatggatgaa gaattattga cagcttctgt ttattctttt
    24601 acgaaacaag tgacaagtac gatttctatt tctcgtaaat ttttatcttc agaagacaaa
    24661 gtattgatta tcgacgattt cttggcaaac ggacaagcgg caaaaggatt gatcgaatta
    24721 tgccagcaag ctggagcgca agtagaagga atcgggatcg tcatcgaaaa gtctttccaa
    24781 gatggacgtc agttacttga agaaatggga ttgagagtcg tttctcttgc gcgtattgct
    24841 tccttgacga atggacaagt tgagtttctt gaggaggacg cataatcgtg gaaaaagaaa
    24901 cacaaaatgg aaaagctgcg gttttagggc tgcagcatct gctagcgatg tatgcaggag
    24961 cagtagcggt gcctcttttg atcggtacag gactaggctt taatcaagaa cagatgactt
    25021 atttgatttc aattgatatc tttatgtgtg gattagcgac attattgcaa ttgacagtta
    25081 accgattttt cggaattggc ttgcctgtcg ttttaggatg tgcgatccaa gcagtagctc
    25141 cactgatctt gatcggaacc aatgaaggag taggagctat ctacggatct attatcgctt
    25201 ctgggatctt tgttgtgtta gtttctggtg tcttttctaa aattaaaaaa ctattccctc
    25261 ctatagtaac aggaacggtt atcaccgtta ttggtttgac tttgattcct gtagcaatcg
    25321 agaaaatggg tggaggtaat gcaacagcag ctgattttgg tgacaaaaca aatctgctac
    25381 ttgcatttgt gacgattctc ctaattgtag gcgtgcaaat gctggcaaaa gggttcattc
    25441 gttcaatcgc ggtattgatt ggtttagtcg gaggaagcat cttagctgct ttcatgggaa
    25501 tgattgatct gagcgcgatt ggagatgcgc cgttgttcca tgttccacaa cccttttatt
    25561 ttggtaaacc aactttcgac gtatggtcga ttttactaat gatcatcatt tccatggtca
    25621 gcatggtaga atcaacaggt gtatattttg cacttggtga cattactggg aaaaatgttg
    25681 gagaagaaga attgaaaaag ggatatcgtg cagaaggact tgccgttatc ttaggcggta
    25741 ttttcaatac attcccttat actggctttt ctcaaaatgt cggattggtt caactttcgg
    25801 gaatcaaaac aagaaaaccc atctatttct ctgcattctt cttgattgtt ttaggattat
    25861 tccctaaaat cggtgcgtta gcacaaatca tccctgagcc ggtacttggt ggaggaatgt
    25921 tagtcatgtt cgggatggtg gcagtccaag gaatccgtat gctgttggaa gttgatttca
    25981 gcaatgataa aaatctattg attgttgctg tttctgttgg atttgggctg ggtttcaatg
    26041 ttacgccgac attattcagt caaatgccgc aaacattgca aatgttcact ggaaatggca
    26101 tcgtaatgag tagcattacg gctatcatat tgaatttgat ctttaatgga ttgaaaaaag
    26161 atgaagaaat attggaagaa gccttcaaga agtaatagag caaaagaaag gttgtgaaat
    26221 cagcgttttg acctgattgt tagagaaaca aacggaaaaa tagttcctcg tattttgccg
    26281 tttgtttgag ctaataccga gggtcagctg attgaacacc gttgaaggaa gaggtcgtga
    26341 gcctgccgtt catctccgag tcacaaggaa caatttcaaa aaaaagcttc tcatgtttgt
    26401 tgaatattgt tccttgtgac cgaggagttg gcaggagaac accgtttatc cataaaaagg
    26461 aatgtgacct aacttttgtc acattccttt tttttatctt taataaacac gaacaataaa
    26521 atgtttttct ttgttaacat tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat
    26581 atgaatgtag aaaaactggg aggaaaaaca atgacagcaa aaatctctgt gattatgggg
    26641 agtacttcag attgggaaac aatgaaagaa gcatgtcaaa tattggatga attcggtgtg
    26701 tcttatgaaa aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt tgaatatgca
    26761 caaaacgctc gaaaaaaagg aatcaaaatc attattgcag gagcaggggg agccgcgcat
    26821 ctgccgggta tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc ggtccaatca
    26881 cgaacattaa atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct
    26941 gtagctacta cagcaatcgg aaaagcggga gcaattaatg ctggactact tgcgatacag
    27001 atgctttcca tgtatgattt agaattggag gctaaattgg ctgaaagacg ttcaatgttg
    27061 gctaaaactg ttatagaaag tagtgatcaa cttggttaaa cctttgttgc ctggttcaac
    27121 gatcgggatc gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg caaaagaaat
    27181 gggctttaaa gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac aagttgcaga
    27241 ttggcacatt gaaagtgtct atgatgacac ctttgcgctg aaagagcttg ccagaagaac
    27301 agatgtcatt acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc
    27361 catgtcattt attcctcaag ggacagactt gcttgcgatt acgcaagacc gcctgttaga
    27421 aaaatctttt ttggaaacga ataacatcgt gattgctcct tatgcaacga tcgtcagtcc
    27481 gacggatatc caagatgcga ttgacgggat cggctatcct tgtgttttga aaaccactcg
    27541 aggcggctat gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc
    27601 catggacctt ttaagagaag gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga
    27661 gatttcgatc atggtggcag gaaatggtca aggagatttc acgacttttc cggtcgtgga
    27721 aaacatccac cacaacaata ttcttcatga aacgattgct ccagccgcga ttgatcagga
    27781 tgtgatcgag gaggctgagc gtattgcccg tgtgattgca gaagcagtga gcctttcagg
    27841 tgttttaggg gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga atgaattagc
    27901 tcctcgtcct cacaattctg gacattattc tattgaagct tgttctatga gtcagttcga
    27961 tgcccatatt cgaggtattt gtggttggcc attaggagaa aaagtacgtt tgataagcaa
    28021 tgcagtaatg gtcaatgttt tgggagaaca actgctggaa agctatcgct tgattgagaa
    28081 taagccagac tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat
    28141 gggacatatc actatcttga cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac
    28201 gaatatttgg gattagagga gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg
    28261 gaatatctgg acggaagaga acaaataccg tgcgtggctc gaagtcgaga ttttagcaga
    28321 tgaagcttgg gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca
    28381 tgcatcattt gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt
    28441 tgcgtttaca cgagcggttt ctgaaacatt gggagaagaa agaaaatggg tccattatgg
    28501 tctaacaagt acggatgtcg tagatactgc ttatggctat ctcatcaagc aagcaaatga
    28561 tattttacgg gaagacttga aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca
    28621 caaatataca gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa ctacttttgg
    28681 attgaaactt gccacatggt attcagaaat gaaacggaac atcgaacgat ttgaacatgc
    28741 ggcaaaagga gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg caaatatccc
    28801 gccatttatt gaagagtatg tatgtaaaca attggggatc cgtccacaag aaatctctac
    28861 tcaagttctc cctcgggatc tacatgctga atatttctca gcgatggcgc ttattgctac
    28921 aagtattgaa cgttttgcaa cagagatacg cggattgcaa aaatccgaaa cacgtgaagt
    28981 agaagaattt tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc
    29041 gatcggttca gaaaatatgg ctggtctagc cagagtggtg cgcggacaca tggtcactgc
    29101 ctatgaaaat gtcacattat ggcatgaaag agatatttct cattcttcag cagaacgaat
    29161 catcttgcct gataccacga ttttattgaa ttacatgtta aatcgttttg gcaatatcgt
    29221 taaaaatcta acggtctttc ctgaaaacat gaagcggaat atgtctgcaa catttggttt
    29281 gatttatagc cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa gagaagaagc
    29341 ttatgatctt gttcagccaa aaactgcgta cgcttgggac aaccaagtgc agttccgccc
    29401 gctgttagaa gaagatccaa ccatcacatc ggtattatct aaagaagaaa tagacgatgc
    29461 ctttgattat catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga
    29521 ataaagaaga gtgagttgca aaattcaatt aaaatagaat agcaaaaata accaaaaaag
    29581 aagcagatcc gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag
    29641 gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag
    29701 gaattaagcg taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag
    29761 caagttttcg agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata
    29821 atgatttttg aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc
    29881 tgtctgataa agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat
    29941 ttttcggaat gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag
    30001 caaatcatta tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc
    30061 ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag
    30121 ttaattctaa tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt
    30181 tcgtttctga taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg
    30241 ttgatttata aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt
    30301 atttgctttt aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat
    30361 gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat
    30421 ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa
    30481 ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat
    30541 cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct
    30601 ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc
    30661 gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat
    30721 gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc
    30781 agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag
    30841 gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg
    30901 cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat
    30961 atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta
    31021 tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag
    31081 tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag
    31141 tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac
    31201 aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc
    31261 ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac
    31321 atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca
    31381 gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa
    31441 ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt
    31501 gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa
    31561 ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt
    31621 gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac
    31681 cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct
    31741 catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat
    31801 cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt
    31861 acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac
    31921 ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag
    31981 gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga
    32041 atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta
    32101 ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt
    32161 tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg
    32221 caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc
    32281 aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga
    32341 gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat
    32401 tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt
    32461 gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca
    32521 tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa
    32581 cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt
    32641 gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa
    32701 gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg
    32761 cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat
    32821 atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga
    32881 cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg
    32941 atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa
    33001 atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac
    33061 gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca
    33121 ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa
    33181 tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat
    33241 ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa
    33301 gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg
    33361 acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca
    33421 aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt
    33481 aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt
    33541 tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct
    33601 gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca
    33661 ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc
    33721 aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt
    33781 gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc
    33841 attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt
    33901 gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat
    33961 cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa
    34021 tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga
    34081 gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact
    34141 gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta
    34201 tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc
    34261 cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag
    34321 gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc
    34381 tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg
    34441 gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat
    34501 tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt
    34561 cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga
    34621 tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg
    34681 ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg
    34741 ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat
    34801 acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg
    34861 tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga
    34921 aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa
    34981 acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag
    35041 aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa
    35101 gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca
    35161 ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat
    35221 tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg
    35281 ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag
    35341 tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg attgatattc
    35401 aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg
    35461 gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa
    35521 aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt
    35581 tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa
    35641 agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta
    35701 gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa
    35761 cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt
    35821 caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg
    35881 gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat
    35941 gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa
    36001 aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga
    36061 aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat
    36121 tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa
    36181 acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg
    36241 tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg
    36301 ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct
    36361 tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga
    36421 gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt
    36481 ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag
    36541 atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg
    36601 aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg
    36661 aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg
    36721 ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa
    36781 aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga
    36841 aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa
    36901 aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg
    36961 tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa
    37021 tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg
    37081 cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag
    37141 atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt
    37201 ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa
    37261 ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac
    37321 tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg
    37381 aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac
    37441 agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg
    37501 tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca
    37561 accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga
    37621 tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag
    37681 tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt
    37741 actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc
    37801 aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg
    37861 ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg
    37921 ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata
    37981 ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga
    38041 tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa
    38101 acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc
    38161 gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga
    38221 tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta
    38281 tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga
    38341 ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct
    38401 tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg
    38461 taagccgact ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt
    38521 gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca
    38581 gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg
    38641 tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa
    38701 acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga
    38761 tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta
    38821 gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat
    38881 aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc
    38941 ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt
    39001 aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt
    39061 gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa
    39121 agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca
    39181 aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa
    39241 agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt
    39301 cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg
    39361 atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc
    39421 ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa
    39481 gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt
    39541 accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc
    39601 tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc
    39661 tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca
    39721 agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa
    39781 tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc
    39841 tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga
    39901 gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg
    39961 acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta
    40021 caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa
    40081 agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca
    40141 acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact
    40201 tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg
    40261 atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag
    40321 taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt
    40381 atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac
    40441 gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt
    40501 ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg
    40561 tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag
    40621 cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac
    40681 caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc
    40741 aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg
    40801 aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga
    40861 tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa
    40921 aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa
    40981 ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta
    41041 cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg
    41101 aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag
    41161 caggtcaaaa gggctataaa t
//
LOCUS       DACGLH010000017        40951 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.018, whole genome shotgun sequence.
ACCESSION   DACGLH010000017 DACGLH010000000
VERSION     DACGLH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.018"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(290..1087)
                     /locus_tag="IXT21_001000"
     CDS             complement(290..1087)
                     /locus_tag="IXT21_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ4868423.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="IXT21_001001"
     CDS             complement(1084..2268)
                     /locus_tag="IXT21_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ4868424.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="IXT21_001002"
     CDS             complement(2293..3540)
                     /locus_tag="IXT21_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ4868425.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="IXT21_001003"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="IXT21_001003"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ4868426.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="IXT21_001004"
     CDS             5520..5834
                     /locus_tag="IXT21_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ4868427.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="IXT21_001005"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="IXT21_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ4868428.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="IXT21_001006"
     CDS             6519..7163
                     /locus_tag="IXT21_001006"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ4868429.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="IXT21_001007"
     CDS             7178..7507
                     /locus_tag="IXT21_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868430.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="IXT21_001008"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="IXT21_001008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ4868431.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="IXT21_001009"
     CDS             8495..9319
                     /locus_tag="IXT21_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ4868432.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="IXT21_001010"
     CDS             9312..9659
                     /locus_tag="IXT21_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ4868433.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="IXT21_001011"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="IXT21_001011"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ4868434.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="IXT21_001012"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="IXT21_001012"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ4868435.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IXT21_001013"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IXT21_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ4868436.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IXT21_001014"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IXT21_001014"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ4868437.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15316..16083
                     /locus_tag="IXT21_001015"
     CDS             15316..16083
                     /locus_tag="IXT21_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868438.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="IXT21_001016"
     CDS             16073..18115
                     /locus_tag="IXT21_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868439.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18396..18545
                     /locus_tag="IXT21_001017"
     CDS             18396..18545
                     /locus_tag="IXT21_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ4868440.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18556..20070
                     /gene="dltA"
                     /locus_tag="IXT21_001018"
     CDS             18556..20070
                     /gene="dltA"
                     /locus_tag="IXT21_001018"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ4868441.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20067..21272
                     /gene="dltB"
                     /locus_tag="IXT21_001019"
     CDS             20067..21272
                     /gene="dltB"
                     /locus_tag="IXT21_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ4868442.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21317..21550
                     /gene="dltC"
                     /locus_tag="IXT21_001020"
     CDS             21317..21550
                     /gene="dltC"
                     /locus_tag="IXT21_001020"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ4868443.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21553..22821
                     /gene="dltD"
                     /locus_tag="IXT21_001021"
     CDS             21553..22821
                     /gene="dltD"
                     /locus_tag="IXT21_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ4868444.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22962..24044
                     /locus_tag="IXT21_001022"
     CDS             22962..24044
                     /locus_tag="IXT21_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ4868445.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24044..24847
                     /locus_tag="IXT21_001023"
     CDS             24044..24847
                     /locus_tag="IXT21_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ4868446.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24917..25657
                     /locus_tag="IXT21_001024"
     CDS             24917..25657
                     /locus_tag="IXT21_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4868447.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25668..26672
                     /locus_tag="IXT21_001025"
     CDS             25668..26672
                     /locus_tag="IXT21_001025"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ4868448.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26717..28561)
                     /locus_tag="IXT21_001026"
     CDS             complement(26717..28561)
                     /locus_tag="IXT21_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868449.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28773..29291
                     /locus_tag="IXT21_001027"
     CDS             28773..29291
                     /locus_tag="IXT21_001027"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4868450.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29419..30102
                     /locus_tag="IXT21_001028"
     CDS             29419..30102
                     /locus_tag="IXT21_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ4868451.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30411..30484
                     /locus_tag="IXT21_001029"
     tRNA            30411..30484
                     /locus_tag="IXT21_001029"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30445..30447,aa:Arg,seq:tct)
     gene            30643..31002
                     /locus_tag="IXT21_001030"
     CDS             30643..31002
                     /locus_tag="IXT21_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ4868452.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31125..32756
                     /locus_tag="IXT21_001031"
     CDS             31125..32756
                     /locus_tag="IXT21_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ4868453.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32818..33459)
                     /locus_tag="IXT21_001032"
     CDS             complement(32818..33459)
                     /locus_tag="IXT21_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ4868454.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33741..34049
                     /gene="rplU"
                     /locus_tag="IXT21_001033"
     CDS             33741..34049
                     /gene="rplU"
                     /locus_tag="IXT21_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ4868455.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34076..34420
                     /locus_tag="IXT21_001034"
     CDS             34076..34420
                     /locus_tag="IXT21_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ4868456.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34433..34726
                     /gene="rpmA"
                     /locus_tag="IXT21_001035"
     CDS             34433..34726
                     /gene="rpmA"
                     /locus_tag="IXT21_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ4868457.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(34819..35967)
                     /locus_tag="IXT21_001036"
     CDS             complement(34819..35967)
                     /locus_tag="IXT21_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4868458.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(35979..36290)
                     /locus_tag="IXT21_001037"
     CDS             complement(35979..36290)
                     /locus_tag="IXT21_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868459.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(36343..36738)
                     /locus_tag="IXT21_001038"
     CDS             complement(36343..36738)
                     /locus_tag="IXT21_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ4868460.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(36774..37118)
                     /locus_tag="IXT21_001039"
     CDS             complement(36774..37118)
                     /locus_tag="IXT21_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4868461.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            37419..37640
                     /locus_tag="IXT21_001040"
     CDS             37419..37640
                     /locus_tag="IXT21_001040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868462.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            37767..38543
                     /locus_tag="IXT21_001041"
     CDS             37767..38543
                     /locus_tag="IXT21_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ4868463.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            38540..38734
                     /locus_tag="IXT21_001042"
     CDS             38540..38734
                     /locus_tag="IXT21_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4868464.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            38736..39020
                     /locus_tag="IXT21_001043"
     CDS             38736..39020
                     /locus_tag="IXT21_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868465.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            39041..39229
                     /locus_tag="IXT21_001044"
     CDS             39041..39229
                     /locus_tag="IXT21_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868466.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            39555..39794
                     /locus_tag="IXT21_001045"
     CDS             39555..39794
                     /locus_tag="IXT21_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868467.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            39800..40486
                     /locus_tag="IXT21_001046"
     CDS             39800..40486
                     /locus_tag="IXT21_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868468.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            40489..>40951
                     /locus_tag="IXT21_001047"
     CDS             40489..>40951
                     /locus_tag="IXT21_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ4868469.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEED"
BASE COUNT        13053 a         7078 c         8279 g        12541 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    18181 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    18241 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    18301 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    18361 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    18421 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    18481 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    18541 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    18601 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    18661 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    18721 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    18781 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    18841 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    18901 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    18961 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    19021 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    19081 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    19141 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    19201 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    19261 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    19321 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    19381 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    19441 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    19501 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    19561 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    19621 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    19681 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    19741 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    19801 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    19861 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    19921 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    19981 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    20041 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    20101 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    20161 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    20221 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    20281 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    20341 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    20401 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    20461 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    20521 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    20581 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    20641 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    20701 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    20761 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    20821 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    20881 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    20941 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa
    21001 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    21061 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    21121 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    21181 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    21241 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    21301 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    21361 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    21421 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    21481 tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt
    21541 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    21601 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    21661 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    21721 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    21781 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    21841 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    21901 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    21961 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    22021 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    22081 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    22141 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    22201 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    22261 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    22321 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    22381 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    22441 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    22501 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    22561 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    22621 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    22681 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    22741 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    22801 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt
    22861 ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa
    22921 tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac
    22981 agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag
    23041 aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca
    23101 gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg
    23161 acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc
    23221 ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag
    23281 ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa
    23341 gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag
    23401 aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac
    23461 gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg
    23521 tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag
    23581 gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa
    23641 atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac
    23701 caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta
    23761 tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc
    23821 cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa
    23881 gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat
    23941 cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac
    24001 ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc
    24061 tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac
    24121 ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt
    24181 cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt
    24241 acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg
    24301 cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact
    24361 tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc
    24421 catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt
    24481 acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg
    24541 gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc
    24601 atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt
    24661 tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta
    24721 tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt
    24781 gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt
    24841 tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt
    24901 tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa
    24961 aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc
    25021 aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt
    25081 gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg
    25141 gcaacttcat cctaagagaa atccggagac attgctttca atttatggac acacaaaaga
    25201 ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga
    25261 agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt
    25321 agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg
    25381 tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc
    25441 agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc
    25501 tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt
    25561 tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat
    25621 agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac
    25681 caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa
    25741 tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca
    25801 gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg
    25861 ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta
    25921 gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa
    25981 tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc
    26041 tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt
    26101 ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag
    26161 taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata
    26221 tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa
    26281 tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg
    26341 atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga
    26401 attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga
    26461 ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt
    26521 tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata
    26581 aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag
    26641 aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata
    26701 tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc
    26761 gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt
    26821 tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa
    26881 ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt
    26941 cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa
    27001 aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt
    27061 cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt
    27121 aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat
    27181 tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca
    27241 ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt
    27301 ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga
    27361 aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga
    27421 taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga
    27481 tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag
    27541 gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag
    27601 tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg
    27661 caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa
    27721 aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag
    27781 ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa
    27841 taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta
    27901 agagaataag tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa
    27961 aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg
    28021 aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag
    28081 catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg
    28141 gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt
    28201 ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct ttgattagta
    28261 aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat
    28321 ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg
    28381 tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa
    28441 taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc
    28501 tccactttat acgtaaataa aaagattctt ttttattata tatgttatta aaaaaagaca
    28561 taaaggtatt cctttctaaa acttgtaatg ttagggtaaa cgttttttaa actaacgtca
    28621 attatttata ataaaaaatg agataaagat gaggaaatag aattagaaac atataattat
    28681 taaagggaaa catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa
    28741 gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta
    28801 taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc
    28861 gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac
    28921 aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta
    28981 ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa
    29041 acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag
    29101 taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc
    29161 tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa
    29221 caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga
    29281 aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa
    29341 catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt
    29401 gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa
    29461 ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa
    29521 gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa
    29581 tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt
    29641 tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt
    29701 ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa
    29761 gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac
    29821 tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt
    29881 ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg
    29941 ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt
    30001 cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt
    30061 gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc
    30121 tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt
    30181 gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta
    30241 gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga
    30301 ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat
    30361 tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag
    30421 ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt
    30481 gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca
    30541 atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata
    30601 aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga
    30661 attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga
    30721 actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa
    30781 tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta
    30841 ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct
    30901 attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa
    30961 ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa
    31021 tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta
    31081 atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg
    31141 tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt
    31201 tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa
    31261 gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg
    31321 gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga
    31381 tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc
    31441 tatttttact tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac
    31501 ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata
    31561 atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa
    31621 cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga
    31681 tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt
    31741 tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac
    31801 aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac
    31861 aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg
    31921 gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc
    31981 aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg
    32041 cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg
    32101 cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc
    32161 cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca
    32221 aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt
    32281 gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat
    32341 tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa
    32401 tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac
    32461 tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg
    32521 gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg
    32581 atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc
    32641 cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa
    32701 cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca
    32761 atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta
    32821 atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga
    32881 gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga
    32941 ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc
    33001 atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag
    33061 tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc
    33121 aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg
    33181 tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac
    33241 ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc
    33301 gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag
    33361 tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct
    33421 taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt
    33481 tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag
    33541 aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta
    33601 acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc
    33661 tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc
    33721 agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag
    33781 ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg
    33841 tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg
    33901 caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt
    33961 tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa
    34021 aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat
    34081 caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca
    34141 tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat
    34201 cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga
    34261 agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa
    34321 tattgctgag attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa
    34381 ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt
    34441 atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg
    34501 tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg
    34561 atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg
    34621 tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg
    34681 taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaataaa taaactgctc
    34741 tatctcttgt gtagcaaggg atggagcttt tttgtaaatt atgggatttt ttttgaccat
    34801 acttttgacc atactttctt agaagttaat ataatttgtt agtttttgtg ttgtttcttc
    34861 ttttttgctg ttagtaatgt gtgtatatgt gtccatagta gtttttatat ttgcgtggcc
    34921 cattctttct tggacttctt tgtgatctgc gcctgcctca tatagcattg atgcatgtgt
    34981 atgtctaaaa ccatgcagac cgatataagg taagccggca cgctcacgat atatattgta
    35041 tctttctgcc actgactgat taattgtgaa attattttcg tcattggtaa acaaataatt
    35101 agctttagta aatcctaatt gaaataagta ctttctttgt tcgagcttcc aaaattttag
    35161 ttgctttata gttttttcat ctaaataaat tattcgattt gattttttgg ttttaggagt
    35221 ttcagatata taatatttat ctgaacgagc tacagttttt ttaatggcca tttcatttgt
    35281 tttaaaatta atattgtccc aagtgagtgc taatatttcg ccgattctac atccgctgaa
    35341 tgctaatagt ctaaagagcg tatagtctct gtttttaaaa tatggattct gttcttgaga
    35401 tacttcattt agaaataatt ccaactgttc ttttgtatat aattttaatt tcttttctgt
    35461 tttaatttgt ggctttggaa taagtaaatt taatgttgga ttatcagatg ttaaaccaat
    35521 atttatagca tacttaaaaa ctttattcat atagtttatg aaaagagggt actgctttga
    35581 agttcctttt tcactccaat catttactac cttttgacaa tatgccgtat taattttaga
    35641 aagcttaata tttccaaatt tgggatatat atgtttatta aagattattt tagtccttga
    35701 atatgaactt tcttttacag tttttttgta gctttctagc cataattctg ctgcttcttt
    35761 gaaagtagta tttgtacttg tgggcattcc tgttttttgt aattctagtt ctaacctaga
    35821 aagtgctgtt tttgcttctt tttgtgttct aaatcctcgt ttagtagtat attttttctt
    35881 tccagttagc ggatcaatac cgagataagt tttaaagtac catgcttttt cgccattttt
    35941 ctttttatat tgttttatca ttgccatcaa tattacccct attcaaattc taattctgcc
    36001 atgctatcat tgaaaaattc gatcagtcct tgttcctcta taaattcaag ccatatttcc
    36061 ttcccgttaa ttaatggaac tcctttttct ttacgaattg tttgatacaa gttagtcacg
    36121 acttgtttcc acatttctaa tacagcttta tctattttct tatcatccca gcgttttttt
    36181 tggggtctat ttttgttttt ttcaatttga taattagtag ctttgatgat aaatgtttcg
    36241 tatccggttg tgcttttttc taaccattct ttgaattctg tatatatcat ttttgttcac
    36301 ctttctattt ttgaacgtat gttctttttt gcttaaaaaa ttttagagag ctgcataaca
    36361 atagatgcga gcttttaata attctttcac aagtggttcg aagcgaacgc ttaaattgtt
    36421 atcttctata aatttcatgt aattgatatt gtgtggttct aattcagact ttgcaatata
    36481 tctatctaat aatttttcaa tcatgaagcg atcagcctca tattccattt ttgaatggag
    36541 agagaatgct aagttatata aaaaataatt atcatgatgt ttagaagcgt gtcctaattc
    36601 atgtaataag gttctttgtc ttctatactt agataaatta gcttttatta ctatggtatt
    36661 catacaagca acataatggc catcggcatc aaggttttca cgctcttcta ccttaacgcc
    36721 gagttcatta attatcattt caatttgact gtccaaatga ctcacctact tatttagtta
    36781 tcttttttct ctaaatatgc ttcaatcata gctgatagta tttccctatc attatcggtg
    36841 agcggttttc cgtcgctact cataacagat gctaaagctt cttccacggt tagttttctt
    36901 tcctctggtg caactcccgc gttaggatca tctgtgcgtc ctagcaaata atctgttgat
    36961 acatggaagt agtcagcaac tttttgcaaa gtttctattt taggactttg tgtcttccat
    37021 ttataaatag agttttttcc aaatcctatt ctattttcta attcagaaat agaaatacct
    37081 ctttttttac agagctcttt aaccctttcg aaaagcatgt caaatcaacc ttcctagagc
    37141 gtacgataat aatttataca aaaagataga aaggtagttg acaaatctat ccaattgtct
    37201 tatatttaac tcgtaagcta atttaataag ctaacaagca acaagaaata cacctaataa
    37261 aataaacgaa ttcgaggtcg gcaaactaag aatgttaatt tttagggttt ttctaggctt
    37321 atttaactgt atataaatat aagacagttg gatagaaatg tcaataaaaa ttagctaatt
    37381 ttattagctt acatatttaa agaaaaggag gaaacatcat gaaacaaaat aaaaaaactc
    37441 cttccgcaaa aggagcttta ataactttaa attttaaaga ccaaaagttt agtgctgttg
    37501 ttgatgaaat tcatccacaa gagcatcaaa aaaattcttt tgcaactgac gagaaaaaga
    37561 ctcagaaacg ctctgagaaa gttcttcgaa taattcaatt tctgttggaa aagattcgtc
    37621 attttggaag tctattttga attctttatt aacagtcttg aatacggttt gtttgaataa
    37681 ggcgtcgaca tccatattaa attcttccat actattttca cctcgctttc tattcaaaat
    37741 tatatcaaag aaaggaatga gcaaaaatga acacaccgca aatttttaac tttgaacaac
    37801 atgaagtaag aacagtaaca atacatgatg aaccattttt tgttggaaaa gatgtggcta
    37861 aagttctagg gtaccaaaat ggtagtagag atattaacag acacgtcgat gtagaagatc
    37921 ggcaaaacta ccaaaacggt acttttgaat cgcctagagg gttaactatt attaacgaat
    37981 caggtttgta ctccttaatt ttaggaagca aacaaccaaa tgctaagaaa tttaaacgtt
    38041 gggtgactag cgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa
    38101 aagtagaaga agctttgctt aatccagata caatcatcca attagcaact aagttgaaag
    38161 aagaacgtac tggaagacta atcgcagaac aaaaaattgc agaatacgaa cccaaaattt
    38221 cgtatttgga tagtatctta tcttctacag attcagtaac tattagtcaa atcgcggctg
    38281 attatgggat gtctccacaa cagatgaaca aactacttca taagctaggt attcagaaaa
    38341 aagtaggcaa ccaatggtta ttatgcaaaa aacacatgag acagggttac acaaaatctc
    38401 atacaactga gattccgaaa tctgatggcg gaacaaaagt tgtgatgaat acaaaatgga
    38461 ctcaaaaagg gcgtctgttc atttatgagt cgctgaaaaa agaaggatat attccagaaa
    38521 tcgatttatt agaggaggaa tgacgatgtc acaagaattt attttgaaag tcagaattca
    38581 attagcaaaa tacggaaagt ctcagaactg gttagctgat actatcggaa tttctagacc
    38641 atatatgtcg gacattatga atgggcgtag aaaaccagat aaacaaatca agccaattga
    38701 ggcagcacta gcagagttag agaaggagaa ataacatgca aataacaatt ccagataatt
    38761 tagtagtttc cgaattaact acacagatta cgaatgctgt tcttaattca ttggacgaac
    38821 gattacacct tatgaacaaa tcagtagagc ttcctccata tccaaacaaa tcagaggtaa
    38881 aaaaagtttt aggcattggt gatgacaaat taacacattg gataagccta ggcttaaaaa
    38941 cacagcagtg gagcaagtta gacatcagaa ttgaacgatc ggaactccaa agatttttga
    39001 aagaaaactt tgagttctaa aggcaaagga gaatgatttt atgtcctaca cattgcaaca
    39061 agaacatcaa attctaggtc tgattaaaca gcgtagaaaa caattacaag atgatcgtgc
    39121 agcgcttaga aaatccgatg agctatcaga tagacaagct gaactaattg cttctgaact
    39181 tgaggatttg agaatgctag aaataaaaaa tagggagatt agattatgaa gaagacagac
    39241 acacttttta taggattcat tttggggtta ttagtgattg tagcgcacca aagtattatc
    39301 ggaggaagct tgttcgcagc attgatggtt ttaatcaatc tgcttgactc aaaagaaagg
    39361 agcaactatg gcacgagaag aagcgctaaa aatcggtaaa gtgattgctg ataattggtg
    39421 ggcaaatagc cgttctatta ttttaagcaa gcaacatatc gaaaagaaaa aagcatggca
    39481 acaaataaaa aagtgactcc gccgaccaaa gcaatgagtc actcaaaaaa tcataactaa
    39541 ggagatttta acatatggaa aatgaacttt ccactctaga tcaatatttg gccgatcctg
    39601 attggggcaa atcgaattta caactggttg ctaacaagta cgaaaaatca attgatgaag
    39661 caattgttgc attgactgat gcagcttcca taaccgctga tgacagtaaa tggaccaaga
    39721 gagacttggg cattctaaaa cgaggcgtta attggcttga agatcagtac agagaagaaa
    39781 caaaagagaa gtgatatgag tgtttaaacc actaatagat tcatactcgg cggtactaaa
    39841 gaaatttaaa ggtaacgaca ttggtgcaac aatcaacgaa gaagtaaata tcgaacggct
    39901 gaaaacgatg tatgacggat atgatggcga tcgagtcatt gaaattcgct ttattgatcc
    39961 acgacggttc acagtacagc aaagaaactt catctatgcg ctcataggcg atattttcat
    40021 cgatacaggc atgccaacgg acttctggaa ggaattcttc tacttccgtt ttgaaggtgt
    40081 cacagggcgc gaaataagcc tcaaagacga atcgaataca accgtgagtg atgccaatat
    40141 cttagcgaat atcatcctag atttcatctt tgaacatcat attcctttca aagaaggata
    40201 tgagatttta ccagcgaatc aagagtatta cttctacaaa tgcattacaa aaagagtctg
    40261 ctgcatctgt ggcaaaacag gagctgatat cgatcacttt gacaaagcgc tgggaagacg
    40321 aaagcgcaaa gaagttgatc attcagagta cacatttgca gcactctgca gaatccatca
    40381 cacagagaaa cacaaaatag gtgtgatcaa tttcaaaaat aaatatcaaa tcaaagggat
    40441 caagttaaac caggaaacaa ttaaaaagtt aaggatagga ggataaattt ggctgagata
    40501 agttggatca aacttaaaac tactatgttt gacgatgaaa aaatacgatt aatccaagct
    40561 gttcctgagt cggatgccat catcgttata tggattcgat tactagtttt agcaggaaag
    40621 actaacgacg atggtctgat atatatccag aggaacatgc cttataccga agaaatgctt
    40681 gctacattgt ttggcaaaaa cgtaaatacg gttcgcttag cgctaactac attggcaaat
    40741 ttcaacatga ttgatctaag cagtgatgga ctaattgcca tcagtaattg ggaaaaacat
    40801 caaaatatcg agggtatgga taaagtaagg ctaaaaaatg ctgaaagaaa ccgtaaatac
    40861 agggaaagaa agagacagga acgtctcaaa ttggaaaatg acgttagcgt gacgtcacgt
    40921 gacggtacag ataaagatat agaagaagat a
//
LOCUS       DACGLH010000018        40815 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.019, whole genome shotgun sequence.
ACCESSION   DACGLH010000018 DACGLH010000000
VERSION     DACGLH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.019"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(266..466)
                     /locus_tag="IXT21_001048"
     CDS             complement(266..466)
                     /locus_tag="IXT21_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4868470.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="IXT21_001049"
     CDS             complement(771..2003)
                     /locus_tag="IXT21_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ4868471.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2257..2826
                     /locus_tag="IXT21_001050"
     CDS             2257..2826
                     /locus_tag="IXT21_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4868472.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3004..3444)
                     /locus_tag="IXT21_001051"
     CDS             complement(3004..3444)
                     /locus_tag="IXT21_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ4868473.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3602..4366
                     /gene="map"
                     /locus_tag="IXT21_001052"
     CDS             3602..4366
                     /gene="map"
                     /locus_tag="IXT21_001052"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ4868474.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4398..5321
                     /locus_tag="IXT21_001053"
     CDS             4398..5321
                     /locus_tag="IXT21_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ4868475.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5397..6536
                     /locus_tag="IXT21_001054"
     CDS             5397..6536
                     /locus_tag="IXT21_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ4868476.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6529..7329
                     /locus_tag="IXT21_001055"
     CDS             6529..7329
                     /locus_tag="IXT21_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4868477.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7329..8156
                     /locus_tag="IXT21_001056"
     CDS             7329..8156
                     /locus_tag="IXT21_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4868478.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8134..8868
                     /locus_tag="IXT21_001057"
     CDS             8134..8868
                     /locus_tag="IXT21_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4868479.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8968..9834
                     /gene="rfbA"
                     /locus_tag="IXT21_001058"
     CDS             8968..9834
                     /gene="rfbA"
                     /locus_tag="IXT21_001058"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ4868480.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9848..10420
                     /gene="rfbC"
                     /locus_tag="IXT21_001059"
     CDS             9848..10420
                     /gene="rfbC"
                     /locus_tag="IXT21_001059"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ4868481.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10442..11470
                     /gene="rfbB"
                     /locus_tag="IXT21_001060"
     CDS             10442..11470
                     /gene="rfbB"
                     /locus_tag="IXT21_001060"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ4868482.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11568..12419
                     /gene="rfbD"
                     /locus_tag="IXT21_001061"
     CDS             11568..12419
                     /gene="rfbD"
                     /locus_tag="IXT21_001061"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ4868483.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12453..14486
                     /locus_tag="IXT21_001062"
     CDS             12453..14486
                     /locus_tag="IXT21_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868484.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14530..15810
                     /locus_tag="IXT21_001063"
     CDS             14530..15810
                     /locus_tag="IXT21_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ4868485.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16020..16826
                     /locus_tag="IXT21_001064"
     CDS             16020..16826
                     /locus_tag="IXT21_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868486.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16838..18058
                     /locus_tag="IXT21_001065"
     CDS             16838..18058
                     /locus_tag="IXT21_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868487.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18048..19634
                     /locus_tag="IXT21_001066"
     CDS             18048..19634
                     /locus_tag="IXT21_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4868488.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19673..21811
                     /locus_tag="IXT21_001067"
     CDS             19673..21811
                     /locus_tag="IXT21_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4868489.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(22179..23162)
                     /locus_tag="IXT21_001068"
     CDS             complement(22179..23162)
                     /locus_tag="IXT21_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ4868490.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTYH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23540..24931
                     /locus_tag="IXT21_001069"
     CDS             23540..24931
                     /locus_tag="IXT21_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ4868491.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQDFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24947..25987
                     /locus_tag="IXT21_001070"
     CDS             24947..25987
                     /locus_tag="IXT21_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4868492.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26006..27091
                     /locus_tag="IXT21_001071"
     CDS             26006..27091
                     /locus_tag="IXT21_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ4868493.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            27124..28086
                     /locus_tag="IXT21_001072"
     CDS             27124..28086
                     /locus_tag="IXT21_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ4868494.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            28079..29506
                     /locus_tag="IXT21_001073"
     CDS             28079..29506
                     /locus_tag="IXT21_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ4868495.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            29508..30578
                     /locus_tag="IXT21_001074"
     CDS             29508..30578
                     /locus_tag="IXT21_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868496.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            30565..31986
                     /locus_tag="IXT21_001075"
     CDS             30565..31986
                     /locus_tag="IXT21_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ4868497.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            31999..33006
                     /locus_tag="IXT21_001076"
     CDS             31999..33006
                     /locus_tag="IXT21_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4868498.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            33018..34022
                     /gene="neuB"
                     /locus_tag="IXT21_001077"
     CDS             33018..34022
                     /gene="neuB"
                     /locus_tag="IXT21_001077"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ4868499.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            34019..35167
                     /gene="neuC"
                     /locus_tag="IXT21_001078"
     CDS             34019..35167
                     /gene="neuC"
                     /locus_tag="IXT21_001078"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ4868500.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            35140..35817
                     /locus_tag="IXT21_001079"
     CDS             35140..35817
                     /locus_tag="IXT21_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ4868501.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            35807..36949
                     /locus_tag="IXT21_001080"
     CDS             35807..36949
                     /locus_tag="IXT21_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ4868502.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            36962..37939
                     /locus_tag="IXT21_001081"
     CDS             36962..37939
                     /locus_tag="IXT21_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4868503.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            37939..38847
                     /locus_tag="IXT21_001082"
     CDS             37939..38847
                     /locus_tag="IXT21_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ4868504.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            38848..39600
                     /locus_tag="IXT21_001083"
     CDS             38848..39600
                     /locus_tag="IXT21_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ4868505.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            39605..40552
                     /locus_tag="IXT21_001084"
     CDS             39605..40552
                     /locus_tag="IXT21_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ4868506.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        13642 a         6218 c         7955 g        13000 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
     2101 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
     2161 aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt
     2221 ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact
     2281 agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt
     2341 actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt
     2401 aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat
     2461 gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc
     2521 cattttgcaa gaagagagaa gaagttgtat cgttcactgt acttagaaga atatggtgga
     2581 ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca
     2641 gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata
     2701 gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt
     2761 gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc
     2821 gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag
     2881 gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa
     2941 tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt
     3001 atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc
     3061 ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa
     3121 agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc
     3181 gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac
     3241 gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc
     3301 ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag
     3361 attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc
     3421 atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat
     3481 gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca
     3541 tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa
     3601 tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt
     3661 agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat
     3721 tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt
     3781 tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc
     3841 tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa
     3901 aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga
     3961 ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg
     4021 gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc
     4081 gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt
     4141 tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat
     4201 cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc
     4261 gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga
     4321 tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat
     4381 aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc
     4441 gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg
     4501 tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt
     4561 taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg
     4621 agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa agttcaggag
     4681 gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt
     4741 tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc
     4801 gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg
     4861 ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga
     4921 actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca
     4981 tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc
     5041 cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg
     5101 ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca
     5161 tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg
     5221 ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt
     5281 tcaataaatt gaaaactaaa ttttctcata aaaaaaagta gaaacaagag ttcttattgt
     5341 aaatttatat cagtaattta taataattat gactcttaat taaagtaggt gcccatatgc
     5401 ttgtttcttt tagtatggtt ataagatttt ttctgacaat tttattatca ctgatattaa
     5461 cacctgtatt caaaatcatt tcagtacaaa caggtatggt cgacaaacca aatgaaagaa
     5521 gaataaataa agttccaatg ccttcagcgg gcggactgcc tatctttatt tcatttgtta
     5581 tatcgacttt attcttattc cgtaaaatta tccctcaatc gtatattgta ccgatcttga
     5641 ttgcttcttt agtaatcata gtcacaggag tgctcgatga taaatatgag ctgacaccta
     5701 aacaaaaaag tatagggatc ttgattgctt ctcttattat ttattttgta gcagatatac
     5761 gtattgattc cttcacgctt ccttttattg gatatatcca actaggctgg cttagttttc
     5821 ccgtaacgat tttttggatc ttcggtatca cgaatgcggt caatctgatt gacggtttgg
     5881 atgggctggc ggcagggatt tcattgatcg gtttaattac gataggaatc attggatatt
     5941 ttttcctcca tgcttctaca gtgtatattc cagttgttat tttctgtctt gtagcaagta
     6001 ttatcggttt ttttccttac aatttttatc cggcaaagat ttatttaggc gatacaggag
     6061 ctctattcct agggtttatg atggccgtcc tttcactaca agggctgaag aacgtgactt
     6121 ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc ggtaacagac acgttttttg
     6181 ctatcattag gagaaaagca aaccgtgttt ctttttctac agcagataaa aaacatcttc
     6241 atcaccgact aatcagttta ggctttactc ataaaggtgc tgtcctgacg atttatgcga
     6301 tggctttgat gttttctttc acagctatgg tcatgaatta tactggaaga atcggaacga
     6361 tcatattgat tattgccatg ttgtttgctg caattttatt atttgaattg attggtctga
     6421 tcaatgagaa acatcagttt attttaaaaa cattgcgatt tttagggaat aaagagtacc
     6481 gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg tcatatagat gaataacaaa
     6541 ttaaaccagc cgatccttat tagtattgtg acggcaaaca gtgataaaat ttttcggaca
     6601 ctagataatt ttatttcaac tgtaaaagat gaaacagcag taaaaatccg aatttttgac
     6661 aacaattcac ttcccgagta caaaaaaaga ctaacagagt ataggaaata tccctacatc
     6721 gatatctatt ttcatgatga aaattccgga tttggttatg gacataatca caatctgata
     6781 gctagcgatt ctcgctatgg cattatttgt aacccagata ttttagtgga tgaagagaca
     6841 gttacagcat tggtcttgtt attgaaagaa catccggaat gtgcgatgtt agcacctaag
     6901 attcttaatg aagatggaac aactcagcac ctcatccgtg agcagctggc tgtttttgat
     6961 tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct
     7021 gtgtttgaat gccgaggatt gcctactgat gctaatagtt atgtacggat gatctcaggt
     7081 agttttatgg tagctgatat tgataaattt aaagagataa aggggtttga tgagcgtttc
     7141 ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta
     7201 ttgtacacac ccttttatgc agttatccat ttttacggga agggtgcgca tcgaaatgca
     7261 aaactgtttc gcgtgttttt gacttctatg atgaaatttt tcaataagtg ggggtggcgt
     7321 tttttctgat gaaaccagaa atattagtag tcgttgtgct ttatcagcaa ctattcagtc
     7381 aatctccagc ttatggacca ttaagcaaag cacttagcga aaagcaaatt cagcttatta
     7441 tttacgataa tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt
     7501 atcatcatga tccgcaaaat cctggtttag cggtggctta taattttgcg ttgtctcaag
     7561 cagatgaagg aacacgttgt ctcgtcactc tggatcagga tacgaggcta gtcgacgatt
     7621 attttgaact attgcgtaaa gtaaccttta cagatgaatg tgttgctgca gttccgatga
     7681 ttttttctgg gagccgacaa gtttctcccc tatattcaga tcgttatatc aatcgtacag
     7741 ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat catggcaatc aattcaggtg
     7801 ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct ttttctttag
     7861 attttttgga tcattggctg ttttggaaag tggatcagtt ggaaaaaact gtggagatcc
     7921 ttcctgtacg aatggaacat gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc
     7981 gataccgttc tattttacaa gcagaaaatc gattttatca tgaatatgat caaaggcatt
     8041 tatccagtca ccgacgacaa ttattgttaa gaacagctaa acagttcttg accgtcaaaa
     8101 accggcatat ctggcggatg actttgcgct catatgttaa gaattggaag gtgtgaattc
     8161 gtgcgttcgg tttgtatagc tacctttaat ggaggcagat atttaagaga gcagttagat
     8221 agtattttat ctcagctttc agcagaagat gaagtagtca tttctgatga tgggtcagta
     8281 gatgatacat gggatatatt aactgaatac gcagaaaaag ataagcgaat ccagcttttt
     8341 tcggggcctc gtcaaggatt gatcgctaat tttggttatg cgatccagca gacgaaagga
     8401 gatctaatct ttttggcaga ccaagatgat gtttggttgg agacaaaagt gaaaaagatc
     8461 actgactatt ttgagcagaa tccggatcag ctagtagtag tttctgattt aatcattgtg
     8521 gacagcgagc tgcaagagat ccatgattcg tatttttcgt ttcgaaattc caaagaagga
     8581 tggcttaata atgtgataag aagtcactat attggtgctg gtatggcttt ccggtcttct
     8641 ttaaaacccg atattttgcc gatcccgtca gaagtgccga tgcatgatat gtggattggt
     8701 ttgctgggag gaaagaagac tgggttccta agagaaccgt taactctgta ccgaagacat
     8761 gatttaaacg cgagtgagat tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt
     8821 gttcatctga ttacggcgtt gatcaaacga aaatggttta aacgttgata aaagatgttt
     8881 aaatccttaa tatttccatg acaaatataa caattgatga gataataaca aagtgaaatt
     8941 tgaaatcaaa aaggagagca gatatccatg aagggaatca ttcttgctgg gggcagcgga
     9001 acacgtctgt accctttaac aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa
     9061 ccaatgattt attatccaat gtctactctg atgttggcgg gaatcaaaga aattctgatt
     9121 atttcaacgc cacaagatac accacgtttc aaggaattat ttggaaacgg aaatgattta
     9181 ggactgcaga tcgaatatgc agtacaagaa agtccagatg gattggcaca agcgtttatc
     9241 attggagaag aatttattgg agatgatagt gtctgcttgg tattagggga caatatttac
     9301 tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt caaaagatac aggcgcgaca
     9361 gtatttggct atcatgtcaa tgatccagaa cgctttggtg tagtagaatt tgatgagaat
     9421 atgcaagctt tgtcgattga agaaaagcct gctcagccaa aatcaaatta cgctgtaact
     9481 ggcctttatt tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa accttcagaa
     9541 cgtggagaac tagagattac ggatgtcaat aaagcatatt tggataaagg aaaactatcc
     9601 gtagaactta tgggacgcgg atttgcttgg cttgatacag gaacacatga atcattgctt
     9661 gaagcatcta cctttatcga aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg
     9721 gaagaaattg cttaccgtat gggctacatc gataaggaac aattgctggc ccttgcacag
     9781 ccattgaaaa aaaatcaata cggtcaatat ttattgaatt tggccgctga ataaggagtc
     9841 cttacagatg aaagtaacag atacaaaatt aacagatgtc aaaatcgtag aaatggacgt
     9901 ctttggcgat catcgcggtt tcttcacaga aagttattca aaagcaaaat ttgcagaaca
     9961 tggattgaaa tatgactttg ttcaagacaa ccattcacta tcagctgaag ctggtgtcct
    10021 tcgggggctt catttccaaa aaggagaagc tgcacagaca aaattgattc gggtagtcac
    10081 tggagcagtt ttagatgtag tagtggatat ccgcaaaggt agcccgactt atggtcaatg
    10141 ggacggattt atcttatctg aacataatca tcgacagttg cttgtaccta aaggatatgc
    10201 ccatggattc gttactttga caccaaatgt gaacttcctt tataagtgtg ataattacta
    10261 taatgcagaa gcagatgggg gcatcgcatt tgatgatcca gatttagcga ttgattggcc
    10321 aattgatcca tcaagagcga ttatgtcgga aaaagatcac cgtcatccga cattaaaaga
    10381 atttgaagca gaaaatccat ttgtttacgg agaaatctaa gaattaagga gaaattttct
    10441 tatgaaaaac atcattgtta ccggaggtgc cggattcatc ggctctaatt tcgttcatta
    10501 cgtggtaaac aatcatccag aagtgcatgt cactgttttg gataaactga cttatgcagg
    10561 aaacaaagag aacttggctg gacttcctag cgatcgcgta gaattggtcg taggagatgt
    10621 tgcagatgca gaattagtgg accgtctagt aaaagaagcg gacgcagttg ttcattacgc
    10681 ggcagaatcc cataacgata attcattaaa agatcctttc ccatttgtac aaacgaatat
    10741 cattggtacg tatacacttt tagaagcttg ccgcaagtat gatgtacgct atcatcacgt
    10801 atctactgac gaggtttacg gcgatcttcc attacgtgaa gacctgccag gacatgggga
    10861 aggcgaagga gaaaaattca ctgcggaaac accatataat ccttcaagtc catattcttc
    10921 tacaaaagct ggttcagatc tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc
    10981 aaccatttca aactgttcaa acaactatgg tccttatcag catattgaaa aatttattcc
    11041 tcgccaagta acgaatatct taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa
    11101 tgttcgtgac tggatccata cgaatgatca ttcttcagca gtgtggctga ttttgacgaa
    11161 aggcagaatt ggcgaaacct atctgatcgg tgcagacggt gaagaagata ataagaccgt
    11221 gatggaattg atcttagaaa tgatgggtca accaaaagat gcgtacgacc atgtaaacga
    11281 ccgtgcagga cacgacttgc gttatgcaat cgattcgaca aaattacggg aagagttagg
    11341 atggaagcct gagtttacga acttccgtga tgggttggct gacacgatca aatggtacga
    11401 agaacatgaa gactggtgga aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa
    11461 tggacaataa tacagagaaa atgtcactgt attaatggaa aaacacatgt gaggcaggcg
    11521 ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga taatcatatg tttttattaa
    11581 ctggaggaaa tgggcaacta ggtacagaat tgcgccattt attagatgaa aaaaatctgc
    11641 aatatgtttc aacagatgca aaagacttgg atatcacaga tgccgagaaa acaatggctt
    11701 acatcacaga attaaaacct gatgttattt tccattgtgc agcttacaca gcagtagata
    11761 aagcagaaga agaagcaaaa gaattagatg agaagatcaa tgttgatggt acaagaaatg
    11821 tagcacttgc agcaaaagct gtgaatgctc aattcgtcta tatcagtaca gactatgttt
    11881 tcgatggaaa aaacaaaaat gtagaatacg aagtagacga ccaaacgaat ccactaaatg
    11941 aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga agagatttta gaagactatt
    12001 atatcatacg aacttcttgg gttttcggga tctatggaca caatttcgtc ttcacgatgc
    12061 aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa
    12121 cttggacacg tactttggca gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg
    12181 gtatttatca tttgtcgaac gaaaatagct gttcttggta tgaattcgca aaagaaatat
    12241 tgaaagatac agatgtagaa gttgcacctg ttacgtcaga agaatttccg caaaaagcaa
    12301 cacgtccgca gtattcagtc atgagcctga aaaagacaga agagttagga tttgtcatac
    12361 caacttggca agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat
    12421 ttccttttat caaatagaat ttggagcatg atatggaaaa acaaacaaga agtgcacgac
    12481 gaaaagaaaa gccaaaagcg aaccaggcag attcgaattt ggtgcttgac ctgtttctaa
    12541 aactccgcta tcttattggc gttttagtca tcgtggtcat cgttactttt aatttaaacg
    12601 gaagttcact tggtgtttgg gataaatatg tgtcgcaaag agacgatggg aaaaaaactg
    12661 atgtgatttt tggacagaat cgggaagtgc ggtcagacga atggctggtg cagacacctt
    12721 tttacctctc acaagctgaa gaggactttc cgttggtgaa cgaggattat tcattaaacg
    12781 gacaaaacat gatcattgcg tataattctc ctgtaaagga tattacggtt atcggaaaac
    12841 cattcaactg gggattcttt ttcctcggaa gagatcgagg actttccttt tattgggcga
    12901 tgaaattagt cgtcatggtc ttgcttggat tcgaagtact gatgatatta acgaagagaa
    12961 aaaaagctct atcgctaatc ggtgcatttg cgctgacatt ttctccagca gtccagtggt
    13021 ggttcatgca acatgtgggc gatttgattt tctttacttt gggactgatg actgcttttt
    13081 atcattattt ctacaatcat gataaaaaat ggctacgaac gttgatgatg cttttggtgg
    13141 ttattttcgg tattgggttt attttagtga tttacccagc ccatcaagtg atgctggcgt
    13201 acttgctgat tttttatttt gttggcctgc ttatttacta tagcaagaaa gtttcatggg
    13261 atctgttgga tgtagtgttg attggagggg cagttttatt tattggcgga gtcatggttc
    13321 atttctggct aacgtcaaaa gacgcattgt tagcttcttt gaatacctta tatccaggaa
    13381 aacgggtaag tactggagga aagtggacga ttggcgaatt tttttctttc cttacaaatt
    13441 ggaaaattcc ttttgaagat atcaactatt cgaataactc tgaagtggcc ctgttctatc
    13501 acttttttcc aactgtattc ctagcaagtc catttgtgtt atttggcaaa aaagatagcg
    13561 aacaaaagtt actgggacgt attttgatga ttttttgtct gttttctatc ttttggatta
    13621 ccgttgggtt gccaaaatgg ctagcagaaa tcactctgct gtcttatgtt cctccattaa
    13681 gagccatcct tacttttagt tttgcggctt gtttattatc gatctggttt atcgcttata
    13741 tttggagaga agatgttatt cctatttggt ataaattcat tttgttggca gtaaatgcat
    13801 ggtcttattt ctatgcgatt acgcattcga aaatggataa ttatttttcg gattttgaaa
    13861 ccatcgcagt tgctgtgttg ggaacagtga ttttagcttt tgcattattt ggctggagac
    13921 gcttgtttta cctcgctttt gccgccttag ttatcgtatc tggctttttc gtcaatccag
    13981 tcgtagaagg tacaggagcg atttttgaaa aaacactggc aaaagaaatc caaaaaatcg
    14041 atcaacaaga tcctggtaaa gtttggttgt ctgaagatga attatataac ttcaccccaa
    14101 cattgggagt gaaatccttc aatacagtac gcttttatcc agatatagct gcatggaaga
    14161 aaatcgatcc gaaagaagaa tacgagaagt attataatcg gtatgctcat acaagagctt
    14221 ttatcgctaa ggaagataca aagttcattt tagatcgtcc agatatgttt tcggtcacgt
    14281 tgaattttga agacgcagaa aaattaggga tcgactatgt gatcagtaaa cgaccattaa
    14341 ctgattacaa ccaattatat gaagcgcagt tcacgcagct atatggaccg gataaagacg
    14401 gttatatgat ttttaaagta gaatatccgg attatccaga tgtaccacaa acacaaactc
    14461 aggaaaatca gctagtcgta cagtaatagg ctagctgaga ataatcctga aatgttagta
    14521 gaaaggagag tggagataat tcaagcaatc gtacaaaaat ttgtcaacaa aatgagtgtc
    14581 ggtttcttgc ttttgttact gacgctagtc ttttttgttt ggacagcagg gttaggtttt
    14641 ccagcaactt tgtttatata taacaatagt tcattactta tcgcttttag tgtggctatt
    14701 ttgctcattt taaatgttta taaagcaaaa ggcgtcgatt ttctttatct aggtgttgcg
    14761 cttgcccttt tcattttctt ttcttacttt cgggaattaa gaaatgagtc tttatttgga
    14821 gacttactgt ttcctttgat tattttattt tgtttagtaa ttaaatggat cgagtggaca
    14881 aaatgggatc gcggtattta tctgattgtc ttttcggtct tcctcggaat cacgttatat
    14941 cgtgtattta cagagatcca agtaccagaa ggacaaagca tctgggctcc aggcaattcg
    15001 ctttctgata tttggatcaa tacgaatata atcggaagtt ctttgatgac tcttgcttta
    15061 ttgatcagtg gatttgccag ttcttttgaa cgatggtatg tacgaatcat cggtgcagta
    15121 gcagttgttg cagcttttct cgggatttgg gtctgccaat ctagaggtgc attagtcgcg
    15181 ttgattgttt ttgcgatatt ggatctttta cctaaatcgt tgatgcgtgt cattcgtgcg
    15241 ccatttattg cttacacggt tacgattctt ttagctttgc cgatttctta tctggcggct
    15301 gtctctgaaa aagtcaattt gtttaccggt cgagaagata tttggcataa attctatcag
    15361 acgctaggag aaaaatctga acaaatttta ctaggaatga aaacatttat cttccaacga
    15421 ggaaaccaat ttttaggaaa ccacaatagt tataattcta ttttgaatat ctatggattg
    15481 ataggatttg ggattgctgc tttgctgctt atccttttta ttggtcgctt aacgttgaaa
    15541 gcagatttat ccaacgggca gatgactttt atctgggcct tttttgcggt gatgatgcag
    15601 tcatttatgg aggatacatt gacttcatac ccatgggttc cgattattta tatcctgttg
    15661 gctatggccg ttcatcgata tgatgaacca gaagttgcgg aggaattcta tgttatggaa
    15721 gaggcatatg acgatatgac ttatgatgat acatcttatg aggaacaacc agatagcgct
    15781 cattcttcca ggatccaacg ctatcattga taggcacaaa atacgagaga ggctgtgaca
    15841 accgtcgcag ccttttttgg tttattgcaa ttgtctagca tcttctgtaa aatagaaacg
    15901 ttagagaaaa tcttgtatca tagaaaatta agaaagaaat aagaacgcag ctcggtacca
    15961 aaaatatggt aaaatggcac ttgactgtaa ttcactgaaa atcgcttgga ggaacacaaa
    16021 tgtttaaaga tttgtattta tttatgaagg acatttatca gaacagaaaa ttattgctac
    16081 agttttcgat caatgacttc aaatcaagat atgcaggatc gtttttagga atcttatggg
    16141 cgtttatcaa tccgctattt actgtattga tttattggct ggtatttggt gttggactga
    16201 aagcacaaat gatcgatgca aaatacccat ttatcgtata tttagttacc gggatgattc
    16261 cctggttctt cttctcagat gcatttgcat caactacggt tgtttttcgg gaatatactt
    16321 atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc cacgacgaaa atcctatcca
    16381 atctgtacac ccatttattc ttcattctga ttggcatagc tgtcgcgctt ataaatggta
    16441 tatatccttc agcaatgtct ttacagctga tttattattt attttgcatg tgtgctttct
    16501 tgacaggatt gacttggctg acggcatcga tccaaccatt tttacctgat atcatgcaat
    16561 taattacgat cgtgctgcag ttgatcatgt ggacattacc gatattatgg agccctaatc
    16621 agtttgattc aggtcttgta agcatattga aattaaatcc gctgtactat gttgttcaag
    16681 gttatcgtga gtcattttta agtgaagctt ggttctggga gcatggaaca tatactttgt
    16741 atttctgggt tttcacgtta gtaatgttcg taatcggctc tgcagtgttc cgtagattga
    16801 aaccacattt ttcagatgtg ctgtaaggag aatagctatg acagaatatg caattagact
    16861 aaaagatatc acaaaatctt ataatatgta cgcaaaacct tcggatcgat ttcgtgaagc
    16921 cctcaatcct tttaagaaat cctatcatga tgtattttat gcgttgaaaa acgtgaatgt
    16981 agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa aacggatcgg gaaaatctac
    17041 aatgttgaaa atcattacgg gagtactgac accaacatcc ggcacgatgg aaatcgaagg
    17101 aaaaatctca gctttgctag aactaggatc aggattcaat cctgagtatt ctggctatga
    17161 aaatatctat ttaaatggaa tggttctagg attttcaaga gaagaagtcg atgaaatggt
    17221 cgatgatatc attgaatttg ctgacatcgg agaccatatt tatcaacctg taaaaactta
    17281 ttcaagcggg atgttcgttc gtctagcttt tgctgtagca atcaatgtga atcccgatat
    17341 tttgattgtg gatgaagcat tggcagtagg cgatcttgaa tttcaactaa aatgtatgga
    17401 aaaatttaca gaaatcaaga actcgggaaa gacgatttta ttcgtttctc atgatatcag
    17461 ttcgattcgt cgtttttgtg atcgttccta ttggttgaaa aacggagaag tcgtcgaata
    17521 tggcgatacg atggaagtaa cgacgaatta tgaaaacttt ttaaagaaaa aatcagttaa
    17581 aacagtggat cggaataaaa accaagtaga acggttcgcc gctccagata tcgtcgacgt
    17641 gacgaaagcg gaattgttgg ataaagacaa aaatccggtg gatatgctgg aacaaggaga
    17701 aaaactatac gttaaagtaa cttatgaagt aaaagatgat acaatcaaaa atccggtttt
    17761 aggaattgcg ctacgaacag tcgacaattt ttatgtatgc ggacttaata ctctactaga
    17821 tggtatcaag attccttgga aaaaaggaga gaatatcttt tatctggagt atgaaaagtt
    17881 aggtttgtta gctggtgaat attattttga cgttgcggtc tttgaagaaa atgcaacggt
    17941 tcctttggta tataagacaa agtacatgaa cttgtttgtc agcggttctt atatcggaga
    18001 aggcatcgtg gtcttagacc ataaatggga agaagggact catagcaatg aaatatgatt
    18061 ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat tgccgcacag atcaaaccag
    18121 gaagtaaagt tttagagttt ggtcccggaa acggccgatt aacgaaacat ttaatcggag
    18181 caaaacaatg tgaagtgagc attgttgagt tagataagga attgttcgat tttgtcagcg
    18241 aatttgctca agatggattt tatggtgata tcgaaagttt tgagtgggca aactattatg
    18301 ctggacagac atttgattac gtcttgtttg cggatgtttt agaacatttg gtagatcctg
    18361 gaaaaacatt gaagaaagtc agagaatttt tgaatgaaga aggagagatc ctgattacat
    18421 tccctaactt ggcgcataat tctgtcatga tcgatttgtt caataaccaa ttgccgtggg
    18481 cttcttatgg tctcctagat gaaacccata attcttttta tactcatgat ggcttccaaa
    18541 aagtttttga aaaagcggga ttattcatta atattgaaga ttatttgtac ttagctgtag
    18601 gtgatacaga attaaaatca acttatgaag aattgccgga agctgtacgc tatgatttca
    18661 aaatgcgacc atttggtgaa gtctaccaat actttttctc attgatgaaa catccagtag
    18721 cacaaagttc cattgcagag ccacaaaatt ctaattatgt aaaagtgtta gaagtaaccc
    18781 agcaaacgaa acaagatgaa acaatccaac aaataccatt caataatttc acaggggaaa
    18841 atgagacact aagtttcccc gtatcagaaa caacagaaag aatggtgttt cgcttcgcgc
    18901 aacaacctag ctttatcgaa ttcagcgcag aagctgccgg agaaaaacta acatttatcg
    18961 attcgaatgc tgtagtcaaa acagtaaatg attgttattt gtttgatggg aaagaattac
    19021 cagagttcgt tctaacggat atttctggga aagaagtgac tatccattgt cactatcgct
    19081 ttatcgggga attgacacca acaatgaaag aattgttgga aacgattcgt cccatggctg
    19141 aagtcacaaa gcagctttct gagaaaaatg acgaattaga aagagaaaat catcatcttc
    19201 ttgaggaaaa tacaagacta gaccatacgt taaaaacaac cacaaatcgc tattgcacat
    19261 tgttggaaag tgatgaatgg acaataaaac atagactagg acgacgtaag aaagaaactt
    19321 cgaaaaaaat ccaagaaaaa gaactttcta tatgtattga cgagaaaatc tgggatgcag
    19381 aaacaaaaat attaaaaatc atcggatggg gaatcgctaa ttcggatcgc cagcccttgt
    19441 cctataagtt atcagctgac caatcgccat ttttcgaagc aattcctgtt tcacgagaag
    19501 aagtcaatca agcagaacag ctcgcaaaag gaacagaagc gggatttgag ttacgaatcc
    19561 tttgcgagca agaacgttca ttcttagtgg aagcagtagc tcaaaatggc caatcatgga
    19621 tcattgaaat gtaaataatg attaacttat aaagataagg agtaaacgaa tcgtgcaaaa
    19681 aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa cgggccactg gcgatttgct
    19741 tattgtgggc tgggcaattg atgaagtgac caaagaaatc cctacgataa aggtagaaaa
    19801 agaaaatgtg attgctgaag caacacatgt cgtaagattg gatatcaatc atttatataa
    19861 tcttgatgtt aagacccaaa gcggttttaa ggtacgttta tcagggaaaa tgagaggaaa
    19921 agccatctta gacttccaaa cagcgagaca tcaaaatggc atagctgtga aattaaacgg
    19981 taaataccct tatgatgacg gcattgaatc ttcatgggaa agaaaaaaaa gattactgaa
    20041 aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa gcgatccgtc gggtcaaatt
    20101 ggaattgaag ccaggatcga tcgactatgc agagtggatc agtcgtcatg agaaaccaga
    20161 tttgaaagag caaaggaaag aagcacaagg atttgcgtac cgtccattga tttccatact
    20221 tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa tgcattgatt cggtactgga
    20281 tcaaacgtat gatcattggg aactatgtat cagtgatgat gcttctactg atccagctat
    20341 ccgaaagtgt ctggaatcct atcaagcaaa agatgataga atcaaagtcg ttttccgaaa
    20401 agaaaatgga cacatcagtc tggcaactaa ttctgcattg gaaatagcag aaggagaatt
    20461 tatagcactc ttagataatg atgatgaatt accgccattt gcattatatg aagtggcaaa
    20521 agtgctaaac gtacatccag aactggatgt gatctacagt gacgaagata aaatcgatgc
    20581 agatggcaat cgctttgatc cgcatttcaa agcagactgg tcgcctgata cattgatggg
    20641 aaataattat atctctcact taggcgtata tcgaacaagc attgttaaag aacttggcgg
    20701 tttccgtaaa ggctatgaag gttctcaaga ttatgatttg gtccttcgtg tgacagaaca
    20761 aatccctgca gatcatattt accacattga ccgagtcttg tatcattggc ggacgatccc
    20821 aggatcaact gcaagtaatg gcgaagccaa aagttatatc tatgattccg gagtgaaagc
    20881 attgacagat gccctgtcta gaagaaacat caaaggaagc gtacgtcctg gcagaatttc
    20941 tggattttat gagattgctt atgacgtctt gcaagaggat ttggtcagtg tgatcattcc
    21001 aacgaagaat ggctatgaag atctaaaaac ttgtgtggat tcgatcatcg aaaaaacgtc
    21061 ttattcaaat tatgaaatca ttattgcgga taacggaagt acagatccaa aaatgcaaga
    21121 attatttgca gaatacaagc atcagctgaa agaccgcttt atcgtagaat tgattgacat
    21181 tccattcaat tattctcgaa ttaacaattt agcagcagaa aaagcaaacg gcaaatactt
    21241 tttattcttg aataacgata cagaagtgat tgagccagat tggatgacgg tgatggtttc
    21301 ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa ctgttttatc cagatgatac
    21361 aacgcagcat gcaggagtat tattaggaat cggtggtgtt gcagggcatg cattgaataa
    21421 ctatgatcga acacattgtg gttatttcgg ccgattggtc attgacgtca attacttagc
    21481 agtcactgca gcttgtatga tggtcaaggc agctgacttc aatgctgtta acgggtttga
    21541 tgaaacatta gaagtagcat tcaacgatgt tgacttgtgc ttgaaagttt atgaactggg
    21601 tcgttacaat gtctatgcac atcaagcaga attgtaccat ttcgaatcta aatcaagagg
    21661 ttatgaagat actccagaaa aacaaagacg ttttgctggt gaaatcaaaa aaatgcaaga
    21721 taaatggcca gcatatattg cgcatgatcc attttacaat gataatttaa caaaagaagg
    21781 aatcggcgac ttttcgttga gaccagatta aactataata aaaagctgtt atgttgtgat
    21841 tcgctcgcac ataacggctt tttatagttt tttgtcaaaa aaaggcagaa ggtgccgact
    21901 caaaaaaata agtcgaggtt gggacagaag tgtttaactc cgagaaataa gaagaaattc
    21961 acgaaaattg cttttcaaat ttttgtgaat ttcggcttat ttaagaagaa gttgcttctg
    22021 ctcccaccgt ttattcgttt ttaaagaatg agacaaaagt gtttcttact tttgtcccac
    22081 gctcttattc atgctcagag cgccaataga tacaaaatca tagccaaagt ttcacgaacc
    22141 ctagctgtca aagaatcgat attttatttt aatataggct attttaaaat aaatggttta
    22201 aggtattcct ctacaaaagc cactctatca attatcatgg gtttaaaata agaagtgatg
    22261 gagataacca gattgttctt agcgtcaata taaagaacat ttccactatt accaattgct
    22321 gaatatattt cgttctcaga atctaatacc caccataaat agccatatct catattttgt
    22381 aagtcttcta aagcactggt tctaggtttg aacatctcgt ttatccacat gggtggtacg
    22441 atttgttttc ctttatacat ccctttattt aggcataata caccaatttt tgccagatca
    22501 cttgccgata aacacaaacc atatcctgcc gtcccgatat ttagtggatc acaaaaccag
    22561 acattggttt taggagactt atccattgta aactgcttat gttcttcagc tgtttcagca
    22621 taataattga catggtttgg tatccctata ggatcaaata gatacttatt ggcaaaatca
    22681 acagttgtta gcttacttgt ctgatacaac aagccagtta atatgtgtaa acagacagtt
    22741 tgatagttga attcgccggt tatacctttt cttcctccca ataaatctag tgatgttttt
    22801 gtccaatctt cacttgaaca gacttttgtc catggatcgc ctttacattt gtagggtgct
    22861 ttcattgtta gcaagtgctt gattgtaata gcgtaaatag ttttctcccc gcgtttcact
    22921 ttatactctg gaaaataatc taatacttta tcatcgatac ttttgattaa tccttgctct
    22981 aaagcaatgc caatcagcaa agatacaata cttttcgttg ctgacatgat atgataagta
    23041 tcgtcttttt tataaccatt ccattcatct gtataaactt ctaccccatc cttatatgcg
    23101 acaatttgac atatatttgg ttgtcgaaaa ccaataaaat catgcaactc ttgtttattc
    23161 ataagatatt acctcacttt cttctataag cataatgtca ttaaaaatcg atttcaattt
    23221 tttcgattct tcacgtaata caggtattct tatggactag acagcgcttt gggaagatgg
    23281 attctctgaa aaatataaag gttcacttta tgagtggata aaaaaagcag caaatacttc
    23341 gacttcttta atttaccaac attagtgaaa atcgattcat ctggagattt gtaatttaga
    23401 gtccttgtct tgattagagg tgtataaagc tttttattag acatttttaa attccattac
    23461 aatttgctta taaactaggt ttttaaaggt gactatggta agataataga gatattattt
    23521 tgtgattagg agtgtatata tgaataaaaa tggagaatgg agtgctgcac gacggatatt
    23581 cattatatta atggatgtta ttgtatacaa tgcagcaata tatagtagtt tcttactgaa
    23641 gtttcgtgga gagatcccat tacgaaattt agaaactttc caacactcag caattattat
    23701 ttcagtgatt tttattgcgt tgaacatttt gttgggtgca tatgtttttt ataatcgaat
    23761 gatcagtgac atcgtgtttg tcacagtcat cggtcaggta ttgatgacac tagggatcat
    23821 ggtagtgact tttgcgggcc gctggtttgc ttttccacgt cttgttatct tactttcatt
    23881 tgttctcggt acgcttttct taagtatcta tcgtattctc gtatataaac tttatttacg
    23941 cgtaagtcga gataaaaagg tcgtcattat tgggttagaa aaggatgtcg caccagcaat
    24001 ccaagatttt ggttcaaaga aaaacaacaa acataaagtc gaagcagtca ttcttgataa
    24061 ctattatgca aacatcaaaa atatgattaa agacatcgat attgtctatc ttgcttctca
    24121 tatcgaagaa actgaaaaaa tcaaaatcta tcaattagtt actaaaaatg aaaaaaaatt
    24181 gtttttgaat acaacatttg aaaacttgac attgatcaat ccaaatatta tgaatttcga
    24241 agatgaaagt attattgaag catctggatt tagaatccaa cctgaatatg aactattcaa
    24301 acgtttgttt gatatcatcg tttcacttgt tttattagtt attgcttcac catttatgtt
    24361 attcacagca attttagtca aagtgacatc accgggacca atcatctata aacaagtgcg
    24421 gatcacaaaa aatcaaaagg aattttcaat ctataaattc cgctcgatga ccgccacagc
    24481 cgaagcaaaa tctggaccag ttttagctaa gagcaatgat gcacgagtaa cgcctgtagg
    24541 taaatttatt cgagctgtcc gctttgatga attaccacaa atcttcaatg tgctgaaagg
    24601 cgatatgtcg atcgtaggac cacgtcctga acgtcctttc ttcgtcgatc agttcaatgc
    24661 agaaaaccct tattactact tacggcataa tgttcgtgct ggtatcactg ggtatgcaca
    24721 agtctatgga aaatatgtct ctgattacaa cagtaaatta cgttttgatt tactttatat
    24781 caaaaaatat tcattgatgc ttgatttaaa gatcttgctg caaaccatca agatcctatt
    24841 tgataaaatg tcctctcaag gacttgaaga agacgaaaat gtgatctcat tggcagaaat
    24901 tgcttttccg aatgaaaaaa tctttcgcta gttgggaggc gtaaaaatga acaatccgaa
    24961 attcagcata atcgtaccag tatacaatgc agaagcaact attggtcgaa cgattgatat
    25021 tctaaaagaa ttggattatg aagagtacga agtcgtttta gtaaatgatg gatcaacaga
    25081 tcaaacacag gaaatcattc aagaactgat tgaaaatgat gaacgttttg aattgattac
    25141 tacgaccaat caagggcccg gattagcaag aaacgaagga ataagacagt caaaaggaga
    25201 atatctctta ttcttcgatg cagatgacac gccagtaaaa acgatcttgt cagattatga
    25261 tcatttgcta gaaaagagtc caaaagcgga tcttgttgtt agttcgtttg tttttcgtac
    25321 aatggacgag ggaagaatcg tttccgaaaa agaaaactta gttacagaat gtcactatct
    25381 aactcacgaa gagtttataa aagacatgta tcagttaatg aatgaacagt tgatgtatgt
    25441 tgtatggaac aaatgctatc gtagcgatat tctgaaaaat aaccaaattc tatttaaaac
    25501 ttataatagt tgtgaggatc gaatatttaa cttacattat tatgaatact gtcatcaagt
    25561 gatcatgaac ccaaaagtag aatatattta tgaatttgaa ggtgggaagg ggatcactaa
    25621 tcaataccga cctaacaagt tcgaaacatt taaagaattc tattcattag caaatactgt
    25681 aacagacgat attaataaag acggtatggc ggcgcttctt ttaaaaggga caacttcagt
    25741 tattttttct atttatagta cctctgcaag aacttcaaaa gagaagaaag acgaagcaaa
    25801 gcagatattg agtgatccag tgatccgaga agccaagcaa attgctaaga cggattcttc
    25861 agcaaaaaaa ataacaaagt tgctttacaa tatgccagct ccactatttt tatctgctgt
    25921 aaaagttgga agcttcgttg aagtgaaaat gccagggtta atggctgctt tgaagcgagt
    25981 atattagtta atggaggaaa atataatgaa aagaattttg cattttcagg gacgaatggg
    26041 tttaggtgga gcagagtctt tcatgatgaa tttatatcgt aaaattgatc gtacaaacta
    26101 tcaatttgat tttttgattt atgaagatta tgcagatgtt caagactatc attcagagat
    26161 tgaaagatta ggaggccgta tttttgttgt acctaatcct aagaaaaata tttttaaata
    26221 tttaatagaa gtgaataagt tactaaaaaa agaatctttt tctatagttc acaatcaggt
    26281 ttattttggc gggggaatta atttatggtt agccaaaaag aatggaatca gacagagaat
    26341 tgctcatagc catgcaactg aagatggaaa atctcaaaat attgtaatga atgtattgcg
    26401 taaattttta accaaattgt tgttacaaaa tgcaacagac taccttgccg ttagtcaaga
    26461 agctggtgaa agtctgtttc aaaaccatcc ctttgagata gttcataatg gtattgattt
    26521 agagctttat agtaaaaatt cagaagctaa ggttaataag agaaaagaat tggatatctc
    26581 aatgagtact tttgttgtgg gaaacattgg acgattagag aaacaaaaaa atcaatctta
    26641 tttgttagag atttttttag aaatactaaa aatgaattct gattcttact tgattttagt
    26701 aggtgaaggg tcacttagaa ataaactgaa agccaaagca gtagaattgg atatagagaa
    26761 aagagtaaaa tttttaggag aaagaaacga tattcccgag ttattatctg tgatggatgt
    26821 ttttgtaatg ccttctcttt atgaaggtct gccaatggtt ggattagaag cacaagcagc
    26881 aaaattaaaa ttagtcttag cagatactat ttccaaagat acaaagttaa caaataatgt
    26941 ggattttgta tcattagatg aaacaccaca agaatgggca aaaaaagtaa taaaagagcc
    27001 gtatgctaat aaaataacac aagaactaaa gagttatgat gtttcgtaca ctactgaaca
    27061 aatggagagt atatatagta gagatcatta aaaatgttca agatatttta ggagtgatta
    27121 tttttgaaaa tatgttattt gatattggca tacaatgata ttggcaactt aaagagattg
    27181 gtcaatcgat tgaacgaaaa tgcagttttt attattcata tagataaaaa aactgatata
    27241 gaaccattta ttgaagcatt taaatcatat gataatgttg aatttttatg taataaaaac
    27301 cgtgttaaaa tttattgggg tggtttttcc attgtacaag cagaaatcaa tttagtaaag
    27361 agagcattac aaaatgagaa atatttaaaa tatgtattat tatctggtgc tgattatcca
    27421 atcaaagata atgagtatat ttataattat tttaaaaaga atagttcagt ggagtttatt
    27481 cgtggaatag atctagatca aattaaacat aaagaatttt attataaaca tatagatgta
    27541 tatcaaaaac atgattatcc tagaattaat aggaataaca caactgcatt taaaattttt
    27601 agagctataa ttaatcgttg tctgaggatg attaaacttc ccccaaaaat tagacaccat
    27661 aaatttgatt tgtatcatgg ttctcaatgg tgggctttgt cgaaagaatg cctaacagaa
    27721 ttaatacaaa tgtatgaaca aaatcaacaa gactacctga attttaaaat aggtatgttt
    27781 gccccagatg aaaaattttt tcatacactt ttttttaatt cctcgttcaa aaataaaaat
    27841 gttattggag gaccagattt accacttgaa ttgaaaaata ttgaagaaac tactttacaa
    27901 acttcgaaat tagcaaatat tcatattatt gatccttcca tgaacaaatg gtttgatgaa
    27961 agggatatag atcaaataaa agaaagtgaa aaattatttg taagaaaagt taaatcggat
    28021 tactctgata aactattaaa tagaattgat aaagaaattt tgaaagttgg aaatatagat
    28081 gaatagaata gtacctgttt tagagaagag tttattaagt cgagatgcgt ttcgagtaat
    28141 aaatttaatc atttgtctgc tttattttat gcctagtact tttaatgtaa ctaatatccc
    28201 gatgaaaata gcttttgctt ggggtggaat tatagttttg tatgaggttt ttataaagaa
    28261 aaattttttt aaaatgcgtt tttcttggct actgattgga gcaatgatta gttttatctt
    28321 aactataatc attaacagag aaaatttttt aattccatct atttataatt ttggatattt
    28381 gctgattact ttgatagtaa tttttcctgc agacttttca caaagtgaag aggaaaaaaa
    28441 gaaaaaaatg gtgtatttta ataatgtttt cattatcata atttttttag ctgcgctaat
    28501 atctatctat caatttattt ttttgatttc ttatcatgtc ccaacaggaa cgccaggatt
    28561 acttgcaaga caaggtttta ttgaacatcg gttatttggt gtctatacaa gtccaaatgt
    28621 tggagctatg tttggatata cttctattat tttaagttta attagtgtat taatttcaaa
    28681 aataacaaaa acgattcgtg ttttttatat gatgaatttt atcgtacaaa tattttatat
    28741 tacactttct tcgtctagag gtgcccaggt agtaatttgt tcttttttag tattatttct
    28801 tttgttgttt gccaattcaa aatttagaaa gagcattaga gaagtactac ctgtaaaaat
    28861 caaatggatg gtaagcatat tattagttct attggtatat ttttcttttg gtactaatta
    28921 tgctaaggat tttttagcaa caattcctgt atcgattgat agaaaagtag aagttataga
    28981 gtcaaatgaa tcagataaaa ctccacttcc agaaaaagaa gtaatattac agcattctag
    29041 tgataactcc gaaatttcag ctggtcgttt tacgatttgg aaagctgcgc ttaaagttat
    29101 aagaccaact ttgttatttg gttatggaga gaccgatttc tatgggaata gcgatgcaaa
    29161 atttttgaaa aaagtagatc ttgatactac agatgtgaat gaactaaaaa gagcacatgg
    29221 aaacatgcat aatggatttc ttcaagtact tgtatcaagt ggtataattg cttttatatg
    29281 tatctatgct ttctatgtgt taaatatctt tagtttactc aaaacatttt ttagaagtaa
    29341 agtgaattat gcgtttcttg gtattgtttt aatttttata ctatctattt ttatagatga
    29401 aatggtggag gctcatgtac ttttcaataa gcgcgatgtc atttcaattg ttttctggta
    29461 ttatttggga agcatttctt acttgtattt aaaaaaggag aattaatatg aagacagctt
    29521 tcattgcttg gactccttta catttgatta atatattaaa tgtttatcaa aatttttacc
    29581 gagaatcaaa aggagattta tttatttatt ctgaatttga aaacgctaga agatatagtg
    29641 agcgtataaa agaattaggg ctatttaatg atgttgtttt tgtgaatcac cggaaaatcg
    29701 gttcttcctt taaacgtaaa attaacttgt taactaacca taatcaattt ttctcggatg
    29761 atttatccgt ttatgatgaa atttttatac agggggaaaa ttatttcaca aaaatacttt
    29821 tctccaatgt ttacaaaaaa aatccagatg taaaactaaa ttatatagaa gatggtttgg
    29881 gtacttattt gaagaagagt atattttcaa caagtaaaaa cgttcaaaga gtttttaaaa
    29941 tattaaataa aaatagcatc ttcaaaaaaa aaattgacga ttattttgtc tattgtccgg
    30001 aattgttact ttatgatgta aaaaatataa aaaaaattcc caaaatagat gaaaacagta
    30061 aattgatgaa aaacatacaa tcaatctttg ggatagaaga agtttttgtc ggagataaat
    30121 gtatcttttt tgatcaacct ttattatctg atggttatgg catagatgaa acagatattt
    30181 tcgctaaagt aaatagtata ttttctggaa aaaaagaaac tttaattaag cttcatccta
    30241 gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag
    30301 tcttgctact tgaaaatgac ttctcttcaa cacttttgat tagtcctata tctacaattt
    30361 ctttttcacc atctatgatg tttgaaaaga cgatcaactc tattatgttg gctaaattaa
    30421 ttttaaatga atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc
    30481 ttttctgtga acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg
    30541 cagaattaag aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta
    30601 gttaagtcca gtttctggta tactataggg aattttttta ttaagggaat ttcattcata
    30661 actattccta tttttgtaaa tattactact caagcagaat atggtttgat taataatttc
    30721 acttcatttg tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat
    30781 aatgcaagtt ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta
    30841 gctacacttt cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat
    30901 gctttttttg atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt
    30961 tttgtaatta gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg
    31021 attttatctt ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca
    31081 ttatattttg atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg
    31141 ataggtttag taatttattt aagaataatg ataaagggtg ctcatttttt tgataaaaaa
    31201 cagtggagat acgctttaaa aatctctttg ccgctaatcc cacattcctt agcaaatata
    31261 gttttagcac aattcgatag aattatgatt aattcttatt taggtcctgc tgcgggggga
    31321 atatattcct acattagtaa cataggtatt attctaagcg taatatggat gtctactaat
    31381 aacgcctggg ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag
    31441 aaagttagta actattattt agtagtcttt actttgatta cgatgattgt aatggtagtt
    31501 tcgattgatt tagcaaaatt tatggctccg aaagactatt attctgggtt atctttagtg
    31561 gttccgataa ccttaggcta tttctttcag tttttatata gtttaccagt taatgctgaa
    31621 ttttatgaga aaaaaacatc gtttattgcg attggtacag tagcaagtgc tatagttaat
    31681 atactattaa acatgatttt catacctagg ttaggaataa ttgctgctgg atacacaacc
    31741 gtatttacgt attttttact ttttattttt cattataata tcgctaaaaa aattacgaat
    31801 aaacaattat ttgacacaaa aaaaatatgt atcataataa tttttgtatc aattttttct
    31861 ttgatacttc atattttaat caatcaaatg ttgattcgtt acgttttgac atcattattg
    31921 ataattagtt tagttatata ttttttaaag attaagaatc gaattataga tgactataat
    31981 tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt
    32041 tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt
    32101 tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat
    32161 ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat
    32221 gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct
    32281 atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa
    32341 aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc
    32401 atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga
    32461 aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct
    32521 gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt
    32581 gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct
    32641 atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact
    32701 ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa
    32761 ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac
    32821 gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct
    32881 actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca
    32941 acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact
    33001 tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc
    33061 ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta
    33121 acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg
    33181 caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg ctaaaaaaat
    33241 tagaattatc tcaatctgat tttattagaa taaaagatta ttgtatacaa aataaaattg
    33301 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
    33361 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
    33421 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
    33481 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
    33541 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
    33601 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
    33661 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
    33721 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
    33781 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
    33841 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
    33901 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
    33961 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
    34021 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
    34081 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
    34141 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
    34201 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
    34261 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
    34321 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
    34381 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
    34441 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
    34501 gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa
    34561 taatagatta cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa
    34621 aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca
    34681 aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc
    34741 taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga
    34801 tattatttat attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa
    34861 atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt
    34921 aataaattta ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa
    34981 tctaaatgaa aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa
    35041 aagcttgaaa aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa
    35101 aacattgaat caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc
    35161 ttattaaaat atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc
    35221 attattgaaa ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa
    35281 aataaaatgc aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat
    35341 agcaaaaaaa aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag
    35401 attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct
    35461 atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt
    35521 aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat
    35581 gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta
    35641 catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt
    35701 ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc
    35761 ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat
    35821 tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa
    35881 agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa
    35941 atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct
    36001 tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt
    36061 tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga
    36121 acctaataca tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag
    36181 aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta
    36241 tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga
    36301 atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta
    36361 ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga
    36421 taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta
    36481 ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg
    36541 acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg
    36601 atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat
    36661 ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt
    36721 agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc
    36781 actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag
    36841 tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta atatgacaga
    36901 tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt
    36961 tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta gactcattca
    37021 agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga
    37081 agttgaaaag gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt
    37141 tgaaataaat tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga
    37201 ggctttaaat ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga
    37261 tgaaataatc gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt
    37321 gaagagaaaa gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta
    37381 tcaataccat gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt
    37441 ttttgtttca aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg
    37501 gattatagat acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa
    37561 tcttgatatt gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt
    37621 tggaacttta agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg
    37681 tgaaaagatt ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga
    37741 aacttctagt atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc
    37801 acatactatt attgaagatg catatttaga ggagattaaa gaatttttca cgcttttgga
    37861 aaagaaaatg gaaccccaat atacttttga aaaagataag tatattatta acttaattaa
    37921 cgacattgag gtaaattaat gatgaaagta attttttttg ggttagggtc aattggaaag
    37981 agacatctaa aaaatctaat aagctatgga aaaaagaatt ctattaattt tgatataaca
    38041 gcttatagta gcagtggaaa aaaagagttt gaagaaataa cgtatattca aaatagagac
    38101 gatttggata cacattatga tatagctttt attacaaatc cgacttttaa gcatctggaa
    38161 acactaaatg taattgttga aaaagttgat tacatatttt tagaaaaacc tgctttttct
    38221 gagagtattg atcttactca atataattat gattacaaaa atatttatgt tgctgctcca
    38281 ctgaggtata aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa aaaagtatat
    38341 tctgtaagag ctatttgttc tacttatctt cctgattgga gaaaaggtac tgattataga
    38401 aataattatt ctgcctttaa atctatgggg ggaggagtag aattagactg tatacatgaa
    38461 ttggattata ttataaacct atttggtttc cctaaatcag taaaaaaaca gtttggtaaa
    38521 tattcgaatc ttgaaattaa cagtaatgat ttagcaacat atatattgag atacgatgat
    38581 aaaattatag aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg
    38641 tttactttag atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa
    38701 aacattgtta aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat
    38761 ttctttgaaa acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa
    38821 gtattgaaaa tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct
    38881 gggtctaagg ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat
    38941 ttcgctttag caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact
    39001 tgtttgaata cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg
    39061 aaaattgtag aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata
    39121 cgtgattcat tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta
    39181 gatttgactt cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta
    39241 aaaaatttag ataaagacgt ctacttttca gttgtttctg caagacgtaa tccctatttt
    39301 aatatggtaa aaagaattga aaatgaagtt actatagtta ataaatcaag atacacatct
    39361 aggcaacaga cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa
    39421 tttctaaaaa aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat
    39481 tatctaattt tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct
    39541 aaatttttag aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa
    39601 aaggatgagc tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa
    39661 tcatttagga gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga
    39721 aaatcttgat atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt
    39781 gatggaagag atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc
    39841 tagtgtggca gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt
    39901 attaatgatt ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag
    39961 ttcttcagcg gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat
    40021 tcgcccattg acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta
    40081 ttgtcattta tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa
    40141 tcaaaatccg aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa
    40201 acaaatcgta ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga
    40261 ctttgtttat atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc
    40321 actggggaaa caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca
    40381 tgcaatcaat gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg
    40441 tgatatccat gactctctag cagatattac taagatccag tcaattggct atcaaccgaa
    40501 atatggtatt ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact
    40561 aagataagat tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa
    40621 aatcaaatct gggatagaag ggctctttgt caaatgatgt tggtaaagag aaatcttaga
    40681 ataatttgga cagaacagaa agaagaaaac ctctttctgt tctgtttatt tggttaaaaa
    40741 tgttttgcca tgacgaatca taattcgtag tttgaaattg taaaagttgc gaagtaaggt
    40801 aagcgcccca tagaa
//
LOCUS       DACGLH010000019        37022 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.021, whole genome shotgun sequence.
ACCESSION   DACGLH010000019 DACGLH010000000
VERSION     DACGLH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.021"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(61..279)
                     /locus_tag="IXT21_001085"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IXT21_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IXT21_001086"
     CDS             complement(545..739)
                     /locus_tag="IXT21_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868507.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2091..3131
                     /locus_tag="IXT21_001087"
     CDS             2091..3131
                     /locus_tag="IXT21_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ4868508.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SSPSTITKDWLE"
     gene            3972..4304
                     /locus_tag="IXT21_001088"
     CDS             3972..4304
                     /locus_tag="IXT21_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ4868509.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4923..5264
                     /locus_tag="IXT21_001089"
     CDS             4923..5264
                     /locus_tag="IXT21_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ4868510.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            5283..5573
                     /locus_tag="IXT21_001090"
     CDS             5283..5573
                     /locus_tag="IXT21_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868511.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            5765..5998
                     /locus_tag="IXT21_001091"
     CDS             5765..5998
                     /locus_tag="IXT21_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868512.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            6064..6303
                     /locus_tag="IXT21_001092"
     CDS             6064..6303
                     /locus_tag="IXT21_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868513.1"
                     /translation="MNCKEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            6389..7060
                     /locus_tag="IXT21_001093"
     CDS             6389..7060
                     /locus_tag="IXT21_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ4868514.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            7113..8762
                     /locus_tag="IXT21_001094"
     CDS             7113..8762
                     /locus_tag="IXT21_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4868515.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDEGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAEKANIKVTNNDGSVVLKENTDYKIENSDYKIENSDSGFVIDFIVNNGQVS
                     DTVANLHGQDLKMTYNMFVNDSAEIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTY
                     GAKFLKADGGLFGTGIKATPLEGAEFAAKNAEGKYYSFCGR"
     gene            9271..9672
                     /gene="tnpA"
                     /locus_tag="IXT21_001095"
     CDS             9271..9672
                     /gene="tnpA"
                     /locus_tag="IXT21_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ4868516.1"
                     /translation="MDNEYRYSKTTVSLVNYHFVFCPRYRRKIFVNSEVDARFKALVQ
                     EIAKENEWNIIAIETDKDHCHLLINVQPTYSPSKIMKIIKGKTSSIIRQEFEHLSKMP
                     SVWTRSYFVSTAGNVSSEIIKRYVENQRTRY"
     gene            9696..10814
                     /gene="tnpB"
                     /locus_tag="IXT21_001096"
     CDS             9696..10814
                     /gene="tnpB"
                     /locus_tag="IXT21_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ4868517.1"
                     /translation="MKKAHKIRIYPNCRQEIQFQKTFGCVRFYWNFLLDQRITNYKKK
                     KENADYKEDKTTYASLKKMEEYAWLKEVEAQPLSQVAMDLNKAFKNTFNSTFGFPKFK
                     SKKYSKKSYRTAMGIKVNRNYFYVSKVGWVKMAETLRFNGKLMNVTISQSKTEKYFAT
                     FLVDIENFQKDPVTESIGLDLGLTHFCITSAGEKIGNERFYRSLEKRLVVEQRKLSRR
                     LEVAKKHNRKLEECKNYQKQRVKVARIHEKIFNQRNDFLHKLSSRITDENQIICIEDL
                     NVTGMVRNHKLAKSIADVSWSEFVRQLTYKCEWKGRTLVKIDRFYPSSQICSSCGHND
                     GKKELDIREWTCSNCGTNHDRDINASINIRTEGLSGIA"
     gene            10880..11245
                     /locus_tag="IXT21_001097"
                     /pseudo
     CDS             10880..11245
                     /locus_tag="IXT21_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            11258..12013
                     /locus_tag="IXT21_001098"
     CDS             11258..12013
                     /locus_tag="IXT21_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4868518.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQKQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSEKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFAFILFLFIKKIRRRSE"
     gene            12010..12837
                     /locus_tag="IXT21_001099"
     CDS             12010..12837
                     /locus_tag="IXT21_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868519.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVAHQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETKTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIIL
                     LTPISDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            12847..13095
                     /locus_tag="IXT21_001100"
     CDS             12847..13095
                     /locus_tag="IXT21_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868520.1"
                     /translation="MKIIKMLAFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINMLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            13126..15225
                     /locus_tag="IXT21_001101"
     CDS             13126..15225
                     /locus_tag="IXT21_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4868521.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IRENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYSGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LDNAANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGIQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNLRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLQSEKNIDNQAVAAVENVSNKTNVVTIGVDPNLPQVIVPRTGSTHLVTILVVS
                     LVLLVLVTLSYVALKFN"
     gene            complement(15261..17312)
                     /locus_tag="IXT21_001102"
     CDS             complement(15261..17312)
                     /locus_tag="IXT21_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ4868522.1"
                     /translation="MKLLNPKTVLAIGTFAATLGFAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNANKVLNDSKAT
                     QKQVDSAKNNLDSAYKGLKKKPAPTVNKAELQALYNRVKSTAKGDFTEESWNDFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPYMVTINHIDNQTGQVIATDQ
                     AQVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSQTITGNTTITFNYNQVHQINL
                     LCNNNYKNEGRVDILKEQIINVTHGESVTLTAPAIQGYVLDDRVEPTNSVTLNNVTES
                     QNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRNDD
                     PSISVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELVWLNNYRQQNGAAPM
                     QFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVDEI
                     LSDTGAVNAMIMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANPIF
                     K"
     gene            complement(17432..17641)
                     /locus_tag="IXT21_001103"
     CDS             complement(17432..17641)
                     /locus_tag="IXT21_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868523.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAIKLNKLAEETIDTNASTK"
     gene            17829..18287
                     /locus_tag="IXT21_001104"
     CDS             17829..18287
                     /locus_tag="IXT21_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868524.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLVNNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGIDLFI
                     GKTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            18284..18526
                     /locus_tag="IXT21_001105"
     CDS             18284..18526
                     /locus_tag="IXT21_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868525.1"
                     /translation="MIASLFIYPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18544..18846
                     /locus_tag="IXT21_001106"
     CDS             18544..18846
                     /locus_tag="IXT21_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868526.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILGAASIITSLFGLFGQTPQGL"
     gene            18916..20970
                     /locus_tag="IXT21_001107"
     CDS             18916..20970
                     /locus_tag="IXT21_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868527.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEESDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESIWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NGLYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQRELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            20970..23579
                     /locus_tag="IXT21_001108"
     CDS             20970..23579
                     /locus_tag="IXT21_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ4868528.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMNMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            23576..26107
                     /locus_tag="IXT21_001109"
     CDS             23576..26107
                     /locus_tag="IXT21_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868529.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            26157..26489
                     /locus_tag="IXT21_001110"
     CDS             26157..26489
                     /locus_tag="IXT21_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868530.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            26490..27083
                     /locus_tag="IXT21_001111"
     CDS             26490..27083
                     /locus_tag="IXT21_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868531.1"
                     /translation="MRVKGQNLVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            27246..29270
                     /locus_tag="IXT21_001112"
     CDS             27246..29270
                     /locus_tag="IXT21_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ4868532.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYVA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            29270..30475
                     /locus_tag="IXT21_001113"
     CDS             29270..30475
                     /locus_tag="IXT21_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ4868533.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGETFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHQGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTGDGTWIDP
                     LPFLNSSKK"
     gene            30491..31135
                     /locus_tag="IXT21_001114"
     CDS             30491..31135
                     /locus_tag="IXT21_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868534.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIHQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDNHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            31146..31952
                     /locus_tag="IXT21_001115"
     CDS             31146..31952
                     /locus_tag="IXT21_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868535.1"
                     /translation="MSKKKGARERFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLH
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            31975..32205
                     /locus_tag="IXT21_001116"
     CDS             31975..32205
                     /locus_tag="IXT21_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868536.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVHLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTADNK"
     gene            32364..33299
                     /locus_tag="IXT21_001117"
     CDS             32364..33299
                     /locus_tag="IXT21_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ4868537.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            33343..33612
                     /locus_tag="IXT21_001118"
     CDS             33343..33612
                     /locus_tag="IXT21_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868538.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            33844..34140
                     /locus_tag="IXT21_001119"
     CDS             33844..34140
                     /locus_tag="IXT21_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868539.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            34142..34564
                     /locus_tag="IXT21_001120"
     CDS             34142..34564
                     /locus_tag="IXT21_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868540.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            34583..36175
                     /locus_tag="IXT21_001121"
     CDS             34583..36175
                     /locus_tag="IXT21_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868541.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            36196..36531
                     /locus_tag="IXT21_001122"
     CDS             36196..36531
                     /locus_tag="IXT21_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868542.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            36677..36871
                     /locus_tag="IXT21_001123"
     CDS             36677..36871
                     /locus_tag="IXT21_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868543.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT        13472 a         5656 c         7317 g        10577 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctaaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta gtggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgatactgg tagtacttaa ttatccttaa ttttaaaacc
     1861 aaattttatt catacgttct tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttcttatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaatttttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gcgtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaat gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattctg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaagtta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataaa aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa actaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt ttgtataaat
     3481 ttagttgttt taaatttaat tgtctgctat gcctgtgatg aattgatttt cgttctgcac
     3541 ttttggacga taaatttgga caattgaaaa tacaaaaaaa aagaaaggcg gtatgaactc
     3601 ggaaggctgg taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg
     3661 ttaacctttc agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag
     3721 tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat
     3781 gcctaagcat aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa
     3841 attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc
     3901 aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg
     3961 tggtttattt tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta
     4021 aacggtgtac cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg
     4081 ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt
     4141 acattgggaa acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc
     4201 caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag
     4261 tcttgcgttg taccgcaaaa gggatggggc gtttaatcag ctgaattgat cattctcata
     4321 acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaagaactt
     4381 agaatcatta gaaaaataag gagtagactt gatctatcaa caatttcctt cggggagtga
     4441 cggggtagat ttttacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcccttgt
     4501 ttggatagtg gtgaaatgaa tttgtgtaaa taatatacac agagtaaaca agagggaatt
     4561 tagaagttag acaagttaga atagcgccta gattaatcat gagcagctac taagggatct
     4621 aggctttgac gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac
     4681 aagaaattgt gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt ggtcattggg
     4741 tcaatcgagc atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc
     4801 gatatttttt atgtattttt aagtaaacga aaaacgataa aatatgaacg agtcctgaat
     4861 aaagtgaaaa aattgatata aaacatagag aaaatgaaat ttacgtaatg gaggaatgac
     4921 aaatgaaaaa ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc
     4981 aacgttggga ttacaaagga tttgtgacgt ttagaagagg cggaaattgt aaaggattag
     5041 atgttttagc attagacgag gatgatttat acgatcaaac attgactgat aatccaattg
     5101 gattcggctt gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga
     5161 atgataatgg agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg
     5221 tcagcgttga aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa
     5281 ttatgaaatt tttcgaagta aaatcaccat actattatgc gttaatcgca gcaaagaatg
     5341 aagaagaatg tgcagaattg tatgaacaag tagtaacaga tatcgaaaat cgtgaagaat
     5401 ttattcgatg tttgcatgag ttaaatcgaa atgaagcaat tcaagaggat gcgaaaaaaa
     5461 ttagtgaaga aacgttacaa ccaatcggta tggaggaagc tacaaaagac atttttacaa
     5521 ttattgatga acaaaaatct tgtgtattaa gcattgattc tacattggtt taatagatcc
     5581 aagattctat aatttttttc aaagtaaaaa gaaattaggt aatgattctc agaccaatta
     5641 cttaggaaaa taaccataca aattagattt tacagataaa ggattagaag gaatcacggc
     5701 gattaataaa aaggtagtat taccttaggc attgagtaag gaatttattt aggaggaaaa
     5761 ttagatggcg cgaccagttt tagatgcgat ggatatggat actttgacaa aaggaaggta
     5821 tgtacagctg ttagctcgtg gttattcacc taaagatgta ttcaaagccc tatctaaagg
     5881 aacagatatg gaaaaagaaa gattacgaaa agaattcgac tattggagag agcataacgg
     5941 aataaaagat ttaaaaccag cacgtccagt tattagacga aaaaaattga agaagtaaga
     6001 ccaaagcgta tggcgtacat aggctttttt attttgataa aaataatgaa aggaaggaaa
     6061 accatgaatt gtaaagaaat cgaaaataga aaaaaagtgt cgaaagaaat ggaagaaaag
     6121 ttactaaaaa caatgaaaca aaaacacctt aaaagattat ctgtcgcgca atatatcaat
     6181 gatatgcagt taacaggaaa agagaaagct tgtttgttag gttcaatgaa aaactttgaa
     6241 cagttgagac gaacttatgt gaaaaaaagt tctaattgtc aactattgtt agaagtctca
     6301 tgacattcat taatagaatt catttttaaa actttttcta tggaaatgag aaaaagtttt
     6361 tttgttttgt ctgaaaggag gcagatggat gaaaaaatgg ttatttggtt ttttaggagt
     6421 cgctttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac atgaaggaga
     6481 tgtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg cacacgcaaa
     6541 gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc aaatgttgtc
     6601 catacaaaaa gaagcaatcg aaatgggcgt caataaacaa gtatttgcac aaattcagat
     6661 tcctgcactt ggtttagcat tgccgatttt taaaggggca aatcaataca ctttgagtct
     6721 tggagcggct acttattttt atgaggatgc cgaaatgggc aaaggaaact atgttctagc
     6781 aggacacaac atggaaatgc caggtgtttt attttcagat attcaaaagt tgtcattagg
     6841 ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa cgtgtaaatt
     6901 tatcgtatct gagtacttta aattaattga tggcgttcct gaagagaact ctttcttgtc
     6961 tttaccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt atacgtcaca
     7021 aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggttgta atcaacgcga
     7081 gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat gtaaaaaaag
     7141 ctggaatgct tgttggaatg ttaggaatca ttgcaggctg ctttcaggga tatcgagcat
     7201 atgcagaaga tgtcacgcaa aaaacgccac cagagaaggt caatatcacg gttcataaat
     7261 taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa aatgatggtt
     7321 atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat tatggtgatg
     7381 ttgagtttac gttaggaaat attacggatc aagtattacc gagggaagat tctgatctaa
     7441 ccaacgcgaa agtggacgaa atcgttaaag atgtagaaga cgagggatct aacagtgagt
     7501 acgttaagaa tgcaaccaat aaaattactt cagctgtaga tgaaaatggt gagatcactt
     7561 ttgctgatca accagcctat gtcaattcca aagggaatgt atatgtggtt tatgaaagta
     7621 aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt gcgccaatga
     7681 ctgataatac aggttcaggt tttttaaaag acattcactt atatcctaaa aatattgttt
     7741 ctaagttgag ttttgaatta acgaaatttg gtgacgatgg cacagctcaa agcaaacaaa
     7801 cacctttaaa aggggcaaaa tttgagttat acaagggcga acctggaaaa ggaacaaaat
     7861 taggagatct tgtatcggat gatcaaggaa agctgactgc tactgatttg acgttaggaa
     7921 aatactattt tgtggaagtt ccttcagaag ttgtagtggg atcagataaa gaaccaacag
     7981 cagatcaata tttattaggc gcagacgctc gcaacgatgc gcacaataaa ttaacctttg
     8041 aaattacgaa tgatggcgtg acaagtgact taaaagcttc atacgtaaac tataaagcac
     8101 cagtgatcga taaaacagta acaaatggaa caggacaaga acattctttc caaattggtg
     8161 acgcagtaaa ttatcaagga acgattcata tcccaacaga cattgcaggc ggagcagacg
     8221 gtatcacagt aaacggagtg aaaagcgaaa cttctcctta cagtgtattt aaatgggggg
     8281 atacagcagg acaaggactt tcttatgtcg ctgaaaaagc aaatatcaaa gtcacaaata
     8341 atgatggttc agtcgtatta aaagaaaata ctgactataa aatcgaaaat tctgactata
     8401 aaatcgaaaa ttctgatagt ggttttgtga tcgattttat cgtaaataat ggacaagtaa
     8461 gtgatacagt tgcgaattta catggtcaag atctaaaaat gacttataac atgtttgtga
     8521 atgatagtgc tgaaatcgca aatccattaa caaatagcgt ggatttcgtc tatagcaaca
     8581 atccattcaa tcaggaagaa caccatgaaa aaacgaaagc cgacgctgta acttatggcg
     8641 caaaattctt aaaagctgat ggcggactat ttggaacagg aatcaaagca acaccgcttg
     8701 aaggcgcaga atttgctgct aaaaacgcag aaggaaaata ttatagcttt tgcgggcgat
     8761 aaaacccacg gttttaaccg tgtggatgat agcccgcact gtactgtaag agcataggga
     8821 aacttgtgct tagtggaata tataaaatct cgtatatccc aacaaagaaa ctgaattgcg
     8881 gggaacccct aaagcgagta aaacgacaac gtagtcttga aataggcaaa cgtgaccgtg
     8941 gcgaaagcag aaaaaattta ctcgatagca tatggttaaa tcctaagtgt tgcgacaatg
     9001 ggcaatctgc agcgaagact cgaacagagt aacgttcaac gactattcct cttgagggaa
     9061 gtacacacaa gcgtgtggaa gtggtttcgc cgtactgggt aaagccaacg gataagatat
     9121 agtctgtgct ggcacgaaag tgtcagaagt tcatcagaga actgcatagg aagtagcgaa
     9181 cctatgtgaa caaactccac ataaaaaatt cttcattctt ttgtgtatat taacattgat
     9241 ttagtacatg gcttaaccta aaattaaggt atggataacg aatacagata ctcaaagaca
     9301 actgtatcgt tagtcaatta ccactttgtt ttttgcccaa ggtatcgtcg gaaaatattc
     9361 gttaattctg aagtagatgc tagatttaaa gcactcgttc aagaaatagc aaaggaaaac
     9421 gagtggaaca tcattgcaat agaaacagat aaagatcatt gtcatttatt aataaatgtt
     9481 cagcctacgt attctccttc taaaatcatg aagataataa aaggtaagac atctagcata
     9541 atccggcaag agtttgaaca cttaagtaaa atgccaagtg tttggacacg ttcatacttt
     9601 gtttcaaccg caggaaatgt atctagcgaa attatcaagc gatatgttga gaaccaaaga
     9661 acaagatatt gattgcgtta aggaggtgtg cagaaatgaa aaaggctcat aaaatcagga
     9721 tttaccccaa ctgtcggcaa gaaattcaat ttcaaaaaac attcggatgc gttcgtttct
     9781 attggaactt tctactagat caacgaataa cgaattacaa gaagaaaaaa gaaaatgccg
     9841 attacaaaga agataagaca acgtatgctt cattgaaaaa aatggaagaa tacgcttggc
     9901 taaaagaagt tgaagctcaa ccacttagcc aagtagccat ggatctaaat aaagcattta
     9961 aaaatacgtt caactctact tttggatttc ctaaattcaa aagtaagaag tattccaaaa
    10021 agtcctatcg aacagctatg ggtataaagg tgaatagaaa ctacttttat gtatccaagg
    10081 ttggctgggt aaaaatggct gaaacgttgc gtttcaatgg taaattaatg aatgtgacaa
    10141 tcagccagtc aaaaacagaa aaatattttg caactttttt agtcgatatt gaaaacttcc
    10201 aaaaagatcc tgtgacggaa tccattggtt tagatttggg tcttactcat ttctgcataa
    10261 cttccgcagg tgagaagata ggaaacgaga gattctatcg atcattagaa aaacgcttag
    10321 tggtagagca acgtaaactt tcaagacgct tagaagtagc aaagaagcat aatcgtaaat
    10381 tagaagagtg caaaaactac caaaagcaaa gagtgaaagt ggcacgtatc catgaaaaga
    10441 tttttaacca acgaaatgac tttttacaca aactgtcttc tcgtataact gatgagaacc
    10501 aaatcatttg tattgaggac ttgaatgtaa caggcatggt tagaaatcat aaattagcta
    10561 aatcaatagc agacgtatct tggagtgaat ttgtaaggca attaacatac aagtgtgaat
    10621 ggaaaggtcg aacgttagta aaaatcgacc gtttttaccc tagctcgcaa atatgttctt
    10681 cttgtggtca taatgacggt aagaaagagt tagatattag agaatggact tgtagcaatt
    10741 gcggaacaaa tcatgacaga gatatcaacg ctagtataaa cattagaacc gaaggacttt
    10801 cggggatagc ttagtaaatt tgcgcccgtt tgggtgtatt acctaagaac cccatgactt
    10861 tagtcgtgtg gagtgtcagg gtggattagt tgatacggat aaagacggtg tcaaagaagc
    10921 agtatgggta gacaatgtag caaatgcagc gatcctaaaa tcagataaag aagggcgctt
    10981 tgaaatcact ggtttaactg aaggtgaata ttcattggaa gaaacaaaag caccaactgg
    11041 ttaccaaaaa ttaacagaag atattgcatt taaagtaaac aaaaactcat ttaaagagga
    11101 aaatcgaatc actgttaaaa ataatcaaaa agcagctatt ccattaacag gtagtaatgg
    11161 tttccaaacg tatgtattga tctcttgctt gttgctagga gcaggggcac tttctgcagt
    11221 tgtttacttt aagaagaaag cctagaagga tcagctaatg attattcgta agcccccaaa
    11281 aagaagagtc atgcacaaac tgatgatatt tttttggttg ctgtcggcag tagcagtcta
    11341 tttaattcca gttgtggaaa caaatgtagc taaatttcaa caaaagcaaa gaattttagc
    11401 cattgaaaat cttgaaaaaa cagattcttc tttggatgtt gcgaaaatag agttaggcga
    11461 tcctgttggt gttcttacaa ttccaagtat ttctttaaaa ctaccaattt atgacggaac
    11521 ttccgaaaaa attttagaga atggtgtagg gattacggaa ggaactggcg atattacagg
    11581 aggaaacggt aaaaatcctt taatcgcagg gcacagtgga ctttataaag ataatctttt
    11641 cgatgatttg ccttcagtaa aaaaagggca aaaattcttt attaaggtaa atgaagaaca
    11701 acatgcctat caaatttatc gaattgaaga agttaaagca gaagaattac aaaagaatta
    11761 tttagattat ctcatgcctg aaaaagacca agataggatt acgttaatga cctgcacgcc
    11821 aaaaggtgtc aactcacacc gctttttagt gtatggtgaa cgagtttcat tcagtaaaga
    11881 agatttaaaa ctacagaaaa aagcaaaaaa taggaaatca atttcattgg attggcgatt
    11941 agcactgcta ttcgcttttt ttgcgttcat attattttta tttataaaaa aaataaggag
    12001 gagatcggaa tgaataagag aaaatggctg tttgtttgtg tagcagctct ctttgcaact
    12061 gcagcttact cacagatatc ttttgctgat agcagtacca ctccaactac cagtcagaca
    12121 acagatagtt caacaactgc aacaagcgac acggacagtt cgaagaacac tgcagaagaa
    12181 acggcagatt caagtacgag ttctactgaa tcagcggaat caacgtcaga aagttcttca
    12241 gagacttcag aaaaactgca agaagtagaa gatacgcaag taatcaagca aaacattcat
    12301 attcaaaaaa ttatttcttc gaatcgaatc aacaatcaag gaaggcaagt agctcatcaa
    12361 gaaggtgtca atggcgcaaa gtttattgtc tacgatatca cgaatatctt agaagaaatg
    12421 acacaagatg ataatgagaa aaatcaatcg gtagaaataa ttgagtcaaa attgaaagat
    12481 cgagcaaaaa aactttcata cgaccagttg aaaaaagtga ctgaaggaga aaccaaaaca
    12541 attgatgatc aggaaggtgt gattgatttt tcaattgaag tacctgcaaa caaaaaacaa
    12601 gcatactata tcgtcaatga atcaagccct gaaaacattt caaatagcga agatattatt
    12661 ttattgacac ctatttcaga tgaaaagggt gaatttttga cggatgtttg gttatatcct
    12721 aaatcaaaga aatcagagcc gaaagaagaa attaaaaaag ttgtatcaac tggtgtaaag
    12781 aaaaactttt ttgataattg ttgggatttt ttcactcatt tattttttaa cgattaggat
    12841 gaaatgatga aaataataaa aatgttagct tttaatctat cttttttagc cgcaacaata
    12901 ttgtgttgcg gtttagtttt ttctcaatta tcaatttatc caagatatca acgattgttc
    12961 gttcattttc taaatatcca gcatgcttcc atgccactag aagtgattaa catgctctta
    13021 atcaatccaa tttttatggc tagtttgttt cttcttgcag gattggtaac gtgggtatta
    13081 ggaaaaaaag aatgagaagc ttacaatcgt ttaacaaaat aatagatgga gggcattatg
    13141 gaaaataaaa ttcattgcac tacgggaaga gtatttactt tagtgttgtt tttgttcctt
    13201 cttctttttg caaggggaac tgcagtccag gccttagaaa aagttgatcc gagtcaaatc
    13261 agagaaaata ataatctggt actttatcat gatagtgatt cattgagaaa tatcgaagta
    13321 actgggatca atggatttaa gatactagat gagtattcaa ctaaaaaaca aaaagtactt
    13381 gcttggccaa aagtcaaaga tttgaaaagc acgtcaaaaa ttgttgccca ttatcaaaag
    13441 gtgggcactt atctaggaaa agaaattgat tgcgacgtaa ctttttctaa ttttgtagcg
    13501 cacgctgggg ttaaccaaga cggattagct aaaacaatta atgggaaaaa gtattataat
    13561 tatttgctct ttgataaaaa catttatcga ggctttttca tgcagtcaat caacgttatg
    13621 aatgtaaaac tagattttaa gtattcagga tcaaaaacaa aagtagcttt gagtgaacca
    13681 cagccagaaa ttaattcaaa aatgagatcg atgagagcag ctggtattga gttgaatgat
    13741 acatttctat ctatttacag tttaaatgga cagtatcagc aacgtgtgtc ttggtataat
    13801 gacaataatg aatctgtcgg tgaacgtaat attgatatga gagaatttgt tgggtatgaa
    13861 aaaatgggta gcttacctta ttttgtcaca acagatacag catgtgcaga atacaaagct
    13921 cctgcgttat caacaaacaa tttaacttca aaagtagtgg gaggacgtgc agaaaaggct
    13981 tatagctcga atgatcattt caccgatgtt gtaggagctg atacttatca cagaagtggt
    14041 gtaacgtata cgcttcaagg cgcttcccca acattcatga ttggcgcaaa tacgaatagt
    14101 atgatgttta gctttgatac tgcattgcta tggacaccac aaccatcgaa gcctacaaaa
    14161 gaagtgttta acaaagctaa tactgaagag gcagcacaca atattgacaa aaaagtgatt
    14221 ccacaaggat cagatgttta ctatcatatt caccaaaagt ttgatgcatt aacagtcaac
    14281 acaatgaaca aatacaaatc atttaaaatc actgatacct ttgacagcaa aaattttgat
    14341 atggtatcgg atgggaaaaa ctatgatggc gcattgtggt atgttgaagc gaatgggact
    14401 tggaaaaaag ctgatacagg aggaaaatta actgtatcag gtaatacagt cacttatact
    14461 gcttctgaca gctttttaga taacgctgca aattacggta gggattatat tttgcagctc
    14521 catatgaaag caaaaaatga tgcaaaagga attgcaccaa atgtggctac tgtaacgttt
    14581 aacaatgatc cgaacaaagg aatacaaaaa acaaatacag tagaaaacta tttctctctt
    14641 aaaccaaata aaaaagtgga acaagatggg aaagatgttg atggtcgaaa tgatggtaaa
    14701 aaaggaacgc ctacagctcc tttaaatgca ggatctgaag tacaatatct agtcacacaa
    14761 aaatggcata caaaaggtgt agacattgcg tcagatcatt acaagcaaga tccagtggaa
    14821 gctcgtttga cgtataaaga aggttctgcg cgagtgatag atatgagtac aggaaaagat
    14881 attacttcag aaggtacact aacctatgat agcaatttaa gaacgctgaa atgggaagct
    14941 tccgctgatt tcttaagtaa aaatctttta gatggacgag aaattcaact gatatttaca
    15001 gctaaaactc cactacagtc agaaaaaaat attgataacc aagccgtagc agcagtagag
    15061 aatgtttcta ataaaacgaa tgttgtaaca attggtgttg atcctaactt gccacaagtc
    15121 attgttccta gaacaggttc tacacattta gtaacaattt tagtggttag cttagtatta
    15181 cttgtgttag ttactcttag ttatgtggct ctgaagttca attaaacaaa aaaacaaggc
    15241 tgaaaatcag ccttgttttt ctatttgaaa attggattgg cactaataaa tacgttgtct
    15301 gaagaaagtg atccatctgc agaaacttca taagcatgag ataacccaac gatgctttga
    15361 ttgttgtaaa gtaagtttgc gttatgagca gcgctatctt tccacataat catggcattc
    15421 actgctcccg tatctgaaag gatctcgtca acgtaaaggt agaaactatt gatattttct
    15481 cctttagcgc cgtagcagcc taaactttct aagagatctt ggaaagtacc gccgccaggt
    15541 cgataatgac tgaagcttac ttgtaattct tttgcacgta tatcagcagc ttgttgaacg
    15601 atatcattaa attgcattgg agctgcaccg ttttgttgac gataattatt taaccataca
    15661 agttcttttt gacgcacata gttgtttaaa tcacttaatg agatattttt gtatttgaac
    15721 gtgatcgttc gattttggtc aacattattg attactcgtt ggccgttttt atctacgcta
    15781 atggaaggat cgtcatttct acacaagaaa taattttgat cagtcatatt tttccaagga
    15841 gtagagaagc tatccccttc gtatactgtt tttgattcac tagagagaac agcgttggta
    15901 tcagcattta catgattaac agtcacatta aatttacgag tgtagttgaa aatataattt
    15961 tgactttcag tcacattgtt taaggttact gaatttgttg gttctacccg atcgtctaaa
    16021 acatatcctt gaatagcagg agcagttaaa gtgacacttt caccgtgagt gacgttgatt
    16081 atctgttctt tcaaaatatc aactcgcccc tcatttttgt agttgttatt gcacaacaaa
    16141 ttgatttgat gcacttgatt ataattgaag gtgattgttg tattacctgt gatcgtttgg
    16201 ctttgagtat cattaccaat cactttgtag ctaaaattat ctttagcgcc ttcgctatat
    16261 ttaaatgctt ttgccttagc ggtgtaagta gaaccagaag tcacttgtgc ttgatctgta
    16321 gcaatcactt gacctgtttg attgtcaata tggttaatgg taaccatata tggcgtttca
    16381 ggtttgtgtt gcaacccttt aaatgcagca tctagactat ttttagcatt atttacttgg
    16441 gcttgagaag ccttagaatc gtctagtact tttttagcat tggataaagc agtctgaaaa
    16501 tcattccaag attcttctgt aaaatctcct ttagcagttg attttactcg gttgtataaa
    16561 gcttgtaatt cagctttatt cacagtaggt gctggttttt tctttaatcc cttgtaagca
    16621 gaatctaaat tatttttagc actatccact tgtttttgag tcgcttttga gtcatttaac
    16681 actttattag cattatttaa agcagtttga aatgcgttcc aggtattgtc ggtatagtca
    16741 ccttttttcg ttcccttaac gctcgtataa agagcttcta aagctgattt attcactttg
    16801 ggtttagagg tgtcagcgcc ataccaaccg ataccttcgt atttccagcc atagtctttg
    16861 gttaaccatt gtacttcttt ccaacttgca gtgtagtgat gtgagccaga ttttacacca
    16921 ggaaggaaaa ggcggtagat cggtttatta tttttttcgg cagaacgcca ggcaggccct
    16981 tcataagtcc agccgtaatt tttagtcaac catttcactt catcccaact agctgtgtaa
    17041 tggtgatcac gaagaccttt attgtataaa cgatagacaa gatgaccacc gctagtcggt
    17101 gcttcccaag cggggccttc ataattcccc cacttaatct tcactaggtg atcacgttct
    17161 tttgtgctag aggtgtaaaa atgttcatgg ttatttggat tataaaggcg atacatgacg
    17221 ttagttgaag cagcatcagc agatccagcg aaaccaagtg tggcagcaaa tgttccaata
    17281 gctaaaacag tttttgggtt taataatttc atattttcaa ctcctaaaaa ttagttaaaa
    17341 tcgattacaa aatcattata tatcggtaaa ccgatatggt caataaaaaa attaagaaaa
    17401 gcctcatgta tttctggctt ttcttaattt gttacttagt tgaagcgtta gtatcaatgg
    17461 tttcttcagc taatttattt aattttatag cattgatgaa tctcatattg tctatactag
    17521 agcgcttatt tgcgagatca ctgaccgtag ttaaaggtac gccagattct atagatatag
    17581 aacgaactga tttaccacta tcaaacagca accattcgat tttcttttta tcagcgtaca
    17641 ttgcaatgct ccctcttgat tttttcttta ttatagctct tttttgtaat aaatcacaat
    17701 atcaagagag aaagttttta tagaaaggtg ggaaattatg agtatccgtt ccccgtgtat
    17761 caaaatgagg tttgttgcta ctttcgttat ttgatatact aaaataaaac aacgaaagaa
    17821 gaaataaaat gaagaaaaag caactatggt ttgtctctat gtttgcaata ttttttatta
    17881 ttttaagtgg atgttcaagt aataagaaat cggaagctaa agaagataat agtttattgc
    17941 aaccaggaac tgtttggaaa gaagaagtag gtggtcttgt taataatttg aaaattatag
    18001 acgatacaac atgggaatat tccgaaagtg tttggcatcc tgaacaagta caaataacag
    18061 ttgagaagct aaaagactat aaaggattag aaagatataa ggtaatcgat tcaggtggaa
    18121 tagatttatt cataggtaaa acagccttaa tagttcccta taacaaagac ggtttagaaa
    18181 cgtttaaatt tattccaaca aacgaaaaag aacatcgaga taaaagtgaa atattaaaag
    18241 aatacagtac taaatctgga tacaagttac aaaagactag cgagtgattg ctagtctttt
    18301 tatttatcct gaaggaggaa tcacagtggc aaaagctaaa acgccatttg aaaaagccgc
    18361 gtctaaaatc ttagaacgtc gcggtgtatc tgaaacacag tggaaacaag aagtccttga
    18421 aaaagcacaa tttgacctat tacggggaaa agacaaggac ttagaaggat atatctatca
    18481 acgtgaacgt gaaaagcttg ttttcgatga aattattaaa cactaaaagg agcgaacaat
    18541 tttatgtttg aatcaattac aatgaatttt ttaacacaac cgaaagtagt agctgcggtt
    18601 gatattttta gagcattaca aactgcagca actagtttaa aaaacaattt gatgattttt
    18661 gctggtgttg cggccgttgc ttccattgtt attggggggc ttttcttcct atttggacga
    18721 agaggagctg aaaacgggaa gatgcacgta ggttatgtat tgatgggggt aggtgttatt
    18781 ctaggggctg cttctatcat aacttcgtta tttggattat ttgggcaaac acctcaagga
    18841 ttataagaaa gtaaaaaagt aggagtaaga agtagtcgat actttttact ccttttttat
    18901 gaaaggagaa tattcatgta ctttacgatt gcaggcaata ccaaaacaga taaagagtta
    18961 attaaaaaag caacgaagga agtaggaaag cggacttctt ttttaccgaa aacaattaaa
    19021 tattctgtca ttgaaacgga agaacagcta catgtgttag ggaaagtact ggaacacgaa
    19081 agtcgatctt ctgttaagtt gactctcacg ttacatgaca cagaaggaaa aatagagaaa
    19141 gctggtacag aatacgtttt tggcccgctg gattatcaaa tttcagaagg tttctatcct
    19201 acattgatgg atttaatccg ccttgatgtg tttaaagaaa cggatgaaac tatgagtgtg
    19261 gaagagtctt atcgaaacga aaaactttta caagttttag aagaagagtt agagagcgac
    19321 caaagaaaag tgaatcaagt gagtgaagtc gaagtaaatg aagaagtacc atgggaacga
    19381 ccaatctttc caaagaagga gtcaaaagaa aaagagacag tctatgttga cgaacaagct
    19441 gatattccga ctcagaaatc gatatttgag gaagagagcg atgtgatggg tcaattggag
    19501 gtagatccct ttgaagaaac aatggaggat gaagagacgg ccgaagtaga agttgatcca
    19561 gttgaagcta atgaagagtc aatatggact gaaggggatt ctcaggaata tattgaacca
    19621 cttatcagtg aagaagtaga gaaaaacgaa acagagatac aaaaggcaac tgtatttgat
    19681 tatattttgg atcaatatcc gccagagcat gaatggatga aagaaaaaaa caaattattt
    19741 ttaaatgggc tttattcgaa atatcaatta ccccaaacct atcaagcatt tttagagagt
    19801 aaagaagaat taatctcaca gggtaaaggc acacttttag aacagttaga gtctgtccaa
    19861 tcaacaaatt ggcatgagct tgcacaagaa gatttattag ccacctttga agaacgtcaa
    19921 caagaaacag atcaagaaat taatgagtat gcgacggaac agtcgtctaa ttgggagaaa
    19981 gcaaaggagg aactaaataa agaagaacag cgagtgattg aagaagaagt acaaaaaatt
    20041 aaagagcgat acgaaaagaa acgcgacaat gcgtattcct tgtgtgtaga aaagatcaat
    20101 aattttactc agacgaatca agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa
    20161 cgtataagag aacgaaaaga agaattttat caaggtttgc gtacagataa gctaaagatt
    20221 agcaaagaat taaatgagca actgaataag ctatatcaaa caacgactga agttcttatg
    20281 gaaaaacacg agcagatcca gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag
    20341 tatgaaattc aacaacgaga attgaaagaa caagaagaaa aacaacggca aaaagaaaaa
    20401 gagcgcgaac aattagaatt aaaaaaacgt gaacaggatc ttgcggaaca aaaaatggcg
    20461 atagaaaaac aacggttaga gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt
    20521 caagaacaaa tggatacatt gcgacaagca cagttaaatt atctagcaca acctcaacca
    20581 gtagctgctg ttcaaacgcc agtgaacaac caagcaaatt taactccttc accacagcgt
    20641 tcctttaacg gatggatggt gggatgtatt gcaagtttaa gcttgcttct aggtggagga
    20701 acagtatttg cttttaatca tatcaatcaa tcttcacagg ctgattctgt ggccacaacg
    20761 acgtcaatca aggaagaagc ccaagaacaa gcaagaaaag agtatgaaca acaattagaa
    20821 gaattattga atcgtaccaa tcagtcaaca acagagtcaa gtatggaaaa tagcaaagag
    20881 agtgatccta gtaaggctaa agaaggatca agcgattcaa ctgtacaatc aaacacttcc
    20941 gaaagttcag aatcaaatga ggtgaaataa tgcgtgttag agaaacaacg agacgaaaag
    21001 acaaacgatt caatttagaa aagaataggt atctttctca tttctatcgc tatactcctt
    21061 caggagaaga gctaccagaa aaacatgaca aacgtatttc tcaaaagatt ttttatagtg
    21121 gcttagctta tcttccttta aactatgttg ttacaagaat accagaagca tttcatcgat
    21181 ttgagattat tccaggaagt ttacaaggag tgaatatttt tcagttacaa gaaatgccac
    21241 agttattcca ttttgattca actggttttt ggaatccttt gaccccaaca acaagtttag
    21301 gcacacgttt agttgtttct acgatttgtg ttgttccagc tgtacttatg ggattgaaaa
    21361 tgaacatgtg gtttcgtcct ctagcagatg ggcaaaaagg agataaccga ttaaaaacgt
    21421 taaaggaaat ccagcaaatt tatccgcaaa taccagaatc aaagggaggt tttcctggtt
    21481 atggcggaat accaattact cattataaaa agtactggta tattcagacg gatacagtaa
    21541 atacttgtat tgttggtact tcccgttcag ggaaaggaca gatagaagtt ttagcaacca
    21601 ttgacaattt gtctcgtgca gaaagacaaa gttcaatggt tgttaatgat ccaaaaacag
    21661 aattatatgt tgctagtaaa gatgaactag aagctagagg gtatgatgtg tatgcattta
    21721 atatcaccga tccactgcaa tcaatgagtg ataatccatt ggcactcatc gtgaaatact
    21781 ggaagcgagg agacatagac acagcgacac agttaaccaa tactttcacg aatacgattt
    21841 attatgatgc caacgcttca gacaataaat actttaacga aaatgcgcaa aaagctgtca
    21901 atgcgcttat ttttgcgtta ttggaacaag cggatcagac aggggattac tccaaattaa
    21961 caccgaataa tctcgtcgaa cttctttctg agattggtgg ttttaattac caagatcctg
    22021 ataatgaatt caagcagctc aatgctttag atgaatttat gaatcagtta ccacctggaa
    22081 acatagcaaa aaaacaatat ggggcaacaa aaattggttc tgataaagcg aaaggcaaca
    22141 ttctatcgac agcaatcaca ggactaaatc catttacact gacgaaaatt gcgaaaatga
    22201 cttctcaatc aaccattgat tataaatcca ttggttttcc gaagtatttg gatctcaaac
    22261 tacacgagag tctgttaaac cagcgaattg ttgtagagtt ttatcatcaa ggaaaaaaat
    22321 ttcatgagga acgtgtcaaa gtaggctata gaggtttttg tgaaattaac tttgattgtc
    22381 atttagaaca gggagatcat gtttgcttaa aatatgatca tcgaggaaaa gaatatgttg
    22441 cttggtatga gttttctcaa agagtattga aagacgaaga gggaaatatc gtttatcgaa
    22501 aaaaacgagg agaaacccat ttgccagaat tagaaaaaga agccgttcta acattagatg
    22561 aggataaaag taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg
    22621 ctatttttat gctcacacct gattatgatc catctaacca tatcattgtt agtatttttc
    22681 ttagtcagtt gtataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc
    22741 accgtaggat tcattttatt ttagatgaat ttgggaatat gccaccaatg gataatatgg
    22801 aaggaatcat gacagtcaca gctggtagaa acatgttgtg ggatttgttt atccaatcct
    22861 atcagcaact ttatgcgaag tatggggaac aaaacgcagc gattatcaaa gaaaattgtc
    22921 agacacatat ttatattatg tcgacagacg ataataccat agaagaattt tccaaaaaag
    22981 tggggaataa gacggttgaa caagagaaca gtaccatcaa ttcattaggt atgaatactc
    23041 atatcaatcg caatgttgat tcagatcgca tcttaactcc tgaaagaatt tctacttttt
    23101 tagaaggtga aacgattgta ctcgctcctt taaaacgaca agatttgaaa ggtcataaaa
    23161 tacggccgta tcctattttt aatcatgggg aaacgaatct tccttatgct tatcaatata
    23221 ttgaagattt caatccatcg tgggatatca atgatataga catacattgt acacatgcgg
    23281 acttggatct taaggcttta caaattgatt acgattattt tctacctgat atctatgcac
    23341 gtcgacttta tcacaatatg cacgatcaaa tagaagagga agctaaagaa aacattttta
    23401 ttaaaatcgt tcaatcttta gacaactcaa cggcacaaaa gttacttatt caattttatg
    23461 aagagaagca aatagatcgt ttgcaagaat accttgaaaa attgcgtcaa aatgaagaaa
    23521 ttacagaggt tataaggaaa cgacttttgc tagaagctga aaaggcggtg aaagaatgat
    23581 cattggagga atattttccg gcattgccga ctggtggtca ggcttatttg gggatgggga
    23641 tgcaaacccc aaaaccgtaa cgaaagtctt acaagattat ggtaattatc tgcagtatca
    23701 agatgtattt agttggattt ggaacacttt tctgtgggga ttgattaaag gattatataa
    23761 tctcaatact ttactagaaa aaaccattta tcaaagcttt gatttaaaag acttattgaa
    23821 cgccgctggg gtaaatgcac tgtttcaaag tttgattagt aaggtattgg ctcttttttg
    23881 tgtcgtcaca ttaatttatt taggcttgaa attttttatg agtaaacacc caccaaaagt
    23941 caaaaatatc ttggttaatt tatttatggc catgatcttg atttttggtg gtagttcttt
    24001 aatcgatgaa gggttaaata tcagtaaaag cttttacgga gacgtcactg cagcaaataa
    24061 agcagataaa acgggatcac ctgcttttca aattattcaa aataatgtga tcgatgtcag
    24121 tacgttacta gcaactgacc cgaataaagt agaatcccta ccaacagata aaagaaatgc
    24181 tttaacagcg aaaaatttta aaacagcgaa tatcaatggg attattactc ctgaacaagc
    24241 taaaggtatg gcaggtaatg ataaaagtgg gttatcagat atacaaaagg atcggttaaa
    24301 ggcattacaa taccgattgg aactagatga taatgggaaa gaaattcctg ttcaaattag
    24361 tgacgaaggc ttagcaaaat atgtctatac ttcagggtat cgtcgataca gtagtaaacc
    24421 aggtattatc ttagctggtg aatcttcttt ggcggtggcc tatttattta tcctatttac
    24481 gatcgttact tgcattattg agctggtatt taaaaaattc tatttagtga ttgccgcgtc
    24541 aacggacttt gagacaggcc aacggatgaa aagtgcgata gaagatattt ctcaatcctt
    24601 cttgttattg gcgtttacga ttttagaatt acgaatctat acattaatgc tttcaggtat
    24661 cggtgatttg catgcggcag gaaagatcaa tggattcttg tatgttgttg ctttaattgt
    24721 gctaactatt gcgctattca aaggctctca atcggttacg aagattttcg gtgttgatac
    24781 gagtctgaaa aacggcagta attctcttct tagcatgttt gccttaggaa atattgcgaa
    24841 aaatgtgggt agcggagcta aaaaccttgc aagtgctggt aagagtggtt taagccattt
    24901 aaatgatatt cgtaaaaatg gatttggcca tcgaaaagat gttacccctg ataacgggga
    24961 agaacaatca gacgataaag ctacaactgt acctaatagt aagaaaacag tttccgaaat
    25021 gttcgggaaa gcgaaaaatg gtgtttctaa agccgcagaa ggtcttggtt acgtcaatga
    25081 acgtgggtta aaaggaacag ctgaagatct tacagaaaaa gccaaagaca ccttagaaga
    25141 gaaattagac ggcacgagag ctaaagaatt ggctgaagct gtgaagaatc catccgaagc
    25201 actagaaaaa caaaaagaaa aagcttcaga agtcaaagaa ggactaaaag atgcgtatgg
    25261 tgaaggacaa gttaatgcag cagtaaaagc taatacaaca accgaaaaag aaaatcaaga
    25321 aggtatagtg attccttcag tcaaagatca ccaagaagcg ttgcactcta atgaaaattc
    25381 gattgaagag aaaaattcca tagaagaaag aaattcagaa gaaggaaaaa atggacagtt
    25441 accaaaaact gatccagaac aatatcgcta taaacctaac agtgaccttg aaaatgagaa
    25501 gcgaatttca gatttatcaa aagtaaatca agaagacaaa gcagaaaatg agggacagtt
    25561 accaaaaact gatccagaac aatatcggta tagatctgtt aactccgaaa atgaaaaacg
    25621 catttcagat ttaccaaaac gaaatcaaga agtacaaacg gatgaggatt ccatcgttct
    25681 gccaaatgat aatggaatcg aagttggaac tgaaaaatca acgaaaattg gtttacctaa
    25741 ggcacaaaat ttaccaatga aagatagtcg tttgaatcaa ttcaaggaga atgatcaaag
    25801 ctttgttcac caatcaactg caggtactgt tcaaatgaaa ccaagtcgtt tgcctgaaaa
    25861 tggaaatctg acaactgttt cttacaattt atctgaaagt ggtgagacag ggaatgcgtc
    25921 cgttcaaaat gttgtgacaa gagaagctgc accaaaacct attcaaaacg ttcatacagg
    25981 ttacaagcca gcaaatgtgg catttactgc accaacaaaa ataaatgtga caagagaaac
    26041 taaaaacagg aacaatgcat tgaaagaaca actattgagg gatatcaaac aggatatcaa
    26101 accataaaag gcctacaagt taggcctttt ctttatatga aaaggagaaa tcaattatgg
    26161 ataaatatat aaacatgaaa aaccctccaa gatttagtgg ggaatggaag tttttcgcct
    26221 ttttcttttt ggatctatta acgcttggtg gttgcttttt ttgggcgaaa atagtcaata
    26281 accttgtcca tttaccactc ttttttgcac tcttgaatta tggcttatgg attgcgatag
    26341 ctctagtgat tgtttggcga ccctataata acccaggtca acgacaaatt tttgcgatta
    26401 tcgagtctat tttttttacg gacaagaacc attatcatgc gttagatcct aaccgaaatt
    26461 atcatggtac gtataaggag gatgaataaa tgcgagtcaa aggacaaaat ttagttgata
    26521 agaaatccat ttttcagttt ggaaaaccag aagaacgatc tatcttggat ttatttcctt
    26581 ataaatcctg tacagaagag ggagtcttag ttactaaaga agatcatttt caacgttatt
    26641 atcgtgtagt ttctacagac gtggaaggat taaatgaaca agagaaacta gaacgaatga
    26701 atcagttgac caccgtcatg cgtacgtatg tgccaacaat taagttagtt agtttgacca
    26761 cagagacgga tttaacggaa caaatcgtgc aaaagcggca attattagat aaaaatcgta
    26821 tggaacagtc cttaggaaga aatctacaac gattaaaaaa atatgaaaag aaattagtcg
    26881 aagaaatcca ggaattgaaa cgagcagaac aagaacgtcc tgacttaagc ttctttttcg
    26941 tcattgaagg aaagacaatc aaggatttac aaaccagaaa ccgtcagtta atgcgatcta
    27001 gtggcatttt agggttaaaa ccactctaca aaaaagaatt gatcaagatt ctgtatcgaa
    27061 tgaacaatat gaatgatgag taggaatgga gaagagagaa tgaaatatcc gttgatatgg
    27121 ttagaaaaag atgttccgaa ggaagagacc actagaaaat ttcctcacac tttaagcctg
    27181 gataagttgg gaaaagggca atcttttcac caagtaaaga atcaaacaag aagaggtgac
    27241 tgtacatgtt agaagcgatt aaaacagctt tttcaccaca aaatgatcaa aaagtacttg
    27301 atccattaaa gcaactagga aatcgggaaa tcgaaatttt aaaatccaaa gggtatgatt
    27361 tagatttttt aaaacaagtg atgccacaag gtggattaaa tttcgatgaa gagtatgtga
    27421 cctatggaaa tggttattca cggtgtgtga cagtttatca ctacacaaaa aatccaacac
    27481 ttttttggtt ggccaatctc atgaacaatc ctcatacagt ggccacactc gatgttagca
    27541 cagatgaaaa gcaaaaagtg attgatcaaa tcaatcgtgc cttagatgaa ttaaatgatc
    27601 gatcagataa cgagcgaaaa gcgactgacc gaaataaaag cgcagaagaa tacctggaac
    27661 tagcggaata tgcccgaaaa ttgacttcta atggggaaat ttctaaacaa gtcaaagtga
    27721 gagtacatgt ttacggaaac acgattcaag aagtagatga acgagcgcag gaactgattg
    27781 aaaatctcaa agggatggat tatcgtgcgg tgacctatct gcataaaacc aaacgagatt
    27841 ggcaatcgat gtttaccatg ttcgatgaac aagctgaatg gttcggaaat atcaaagggc
    27901 aatcactacc atcgttaaac attggcggag gtttcccgtt ccaccaccaa acgctaaaag
    27961 atccaggagg tacacaatat ggggaaacca tgacaggcgg cccatttgtc tggaatgcga
    28021 cgtataaaaa tagtgtacgg ttgtctacta ataccttgct tctaggaatg atgggagctg
    28081 gtaaatcgac cgttcttaaa atgatcgcgg aagcccattt agctgcagga gactttattt
    28141 ggggctttga aaagggaaaa gactttattc ctttcttaaa ggaatataac ggtattatgg
    28201 tgcgcttaga cggatctgac gggatgatca atcctttaga aatatttgcc acacggacgt
    28261 atgatgaagc atccagttta tatgacgatg gatcagtcaa agatttgaag atcaatgaag
    28321 cagcgtccta tcaaacacac ttagacaaag tggtctatca ggtacagtta gtgagtcctc
    28381 agctaaaagg aacgatgaaa gcagaattta agacctactt aaatcgtttt tatgaagagt
    28441 atggaacagt accacgcggc tttacttcct caaattcgcg ttcaaatacg gagacgcaag
    28501 tcacagggaa agatccagaa gcttatccga cattcaaaga atttttagac tttttaggac
    28561 aactggaatt accaggtgct tcccaagaga aaaagaatcg aaaagaagaa atggaatcaa
    28621 tcgtagaatc cttatgtgag acgtatggca tgatttttga tggtcatagt acgattcgac
    28681 accttgacca acaacagttg gtcttttttg acattgactc tattacaggc ttagatcgag
    28741 gggtacaaca atgtcaaatg tacgtggcga tgacgttgat ttggaatcag gcattgattc
    28801 aaggaagaag acaacgacgt ttaattaaag aaaaacaaat ggaagaacaa gacttcaaac
    28861 gcttcttggc gatgattgat gagtgccata atgcgattaa tccagatttt attgaaacgg
    28921 tcaattatgt cacaaacttt caacgagaaa tgcgtaaagt agaagcaatg actgttttag
    28981 ctacacaatc cccacaagaa atggtgccag aaaatatgtc ttctgataac ttctcgaaac
    29041 tgaagaaagt ctttgaattt tcttctacga agatctttat gcgtatggat gaatctattt
    29101 tacgtcatat agaaggatta gttggtaaat caatgacgaa ctcagaatta gaatccattc
    29161 ctcaacaaaa tcaaggagac gctgtgatca attttggctc aaaagaaacc attcgtgttc
    29221 attttagtcc gaacaaacgt caattaaaat tgtttgatgg aggaaaataa tggaaaacca
    29281 aaatgaaagt ttaatcaagc agtatgttaa gcgtcgtgca aagcgacgct tatttttgtg
    29341 gttgtttggt acttctgcag gtttgattac cattttgatc actgtattcg tgaccctatt
    29401 tttaatactt gcggctggtt cgattgataa ttctgattca gattcaagca gtggtggcga
    29461 aacttttaca ggagagtatt cggaaggatt gcccatttat aaagaaatca aaggaagagg
    29521 ccctttttct gatgagattg cgcaatatgc cgtaggtgca gcagtgaagt ataaattatt
    29581 accaagtgtg atcctttcgc aatacgggta tgaatcagcg tttggtacgt cggcgtctgc
    29641 aagaaatgat ctcaattatt ttgggattac ttggttcgat ggctgtttat ttccaaaagg
    29701 aactgcgcgt ggaattggtg gcattgaagg tggctggtac atgaagtttc ccaactcaaa
    29761 agctgccttc tcctattacg gctttatggt agccacacaa tccaatttca atgcttgtgt
    29821 aggaaataaa agtcctggcg caagcttatt gatcttagga cgagggggtt atgccgctgc
    29881 aggaattacg gaagatagtc cgtattatac aggttgtatg tccattatca cttctaataa
    29941 attaaccgaa tatgatgaat tcgccatcaa acattggggt gaaggtggca ataacaacgg
    30001 cacgatcact ggtgaatgga cgaatccatt ccctggcagc agtttagata aaagttcctt
    30061 cagtggtggt caattatttg gaacaaatcc aggcggtgaa ttccgaccaa atggtttcca
    30121 tgatggatta gactttggtt cagttgatca tcaaggaagt gagattcatg cggttcatgg
    30181 aggaaaagtc gtgtacgtcg gaaatcctgg tatttcaggt ttgggcgcgt gtgtgatcgt
    30241 gattaattac gacggcttga atatggtata tcaagaattt gcaaatagta caggaaattc
    30301 ccgtgtgaaa gtgggagatc aagtgaaagt tggtcaagta atcgggatac gagatacagc
    30361 acacttgcat ttaggtttca ctcgaatgga ttggcgtcaa gcacaaggac acgcattcac
    30421 aggtgatggt acatggatcg atccattacc atttttgaat agcagcaaaa aatagatcag
    30481 gagaaaaaat atggaaaata aaactttaaa aatcgtctta acagctgcac tcagcattag
    30541 cttaattggc aatatcgcgc tcttttggca gctgaaagag gaacaagcga caaataccta
    30601 taaaattgaa gaagtggcta aaaacgcgtc tgagaagctc aaaaaaaatt cctctgaggc
    30661 acaggcgaag atcgacaagt taaatcaaac catcaatgag aaaaaccaag aaatccatca
    30721 gctgaagcaa caagatggca caaaagcaaa agaggaagta actgaagcac aaagaaagac
    30781 ggcagaagag ttcggtcaat tggccattga taaatcatta agcagaaatg aattaacgga
    30841 aaaactaaaa gatgtcgcaa cccaagaagt gatcgacaaa ttaagtcctg cagacgataa
    30901 tcaccaacat gatgattacg gaagttactc ttttacatta ggtgacgtgc aaacttacgc
    30961 tcaaacaagc agtgtaaaag aggaacaaaa ttttgttgta tttgttgatt atacaatcgg
    31021 caatcctcaa ttcaaagatg tcaaaccgca aaaaataaaa ggtggattaa ctgtcactga
    31081 aaaacaagta gatggtcaat ggaaagtcac agactttagt tattttactc gttaggagga
    31141 agaaaatgag taagaaaaaa ggagcaagag aacgtttcaa aaaacgaaaa agttatctta
    31201 gatggctggg aatctttatg attctcggct ttttaattct tgctagtccg atttatacgc
    31261 ataagaaggt agacatacca gatgaggtaa gcacgaacgc atttttaaac acgaaggaac
    31321 tgacgatggt ttcaaaagaa aaaggagcga accagcaatt agtttttgaa tttgcgatcg
    31381 acgatgaaga tcaaagctta ttaaaagaac tggctaatct taattatcgc gtagaagtaa
    31441 agacagctaa aggggattat caagcaataa aagccaaagt gatcaaggtg tctgatgatt
    31501 attttgtagt gaagttagca catgtaccag aagaatatat cgccatgcgt ttaaccatta
    31561 ttcctgaaaa aattgatcct aaagtggata tgcaagaacc acaagatctg atttattacg
    31621 ttcacgaaga taaggttaaa gatcaagtga aagatgaaga ttatgaacaa catgcgatca
    31681 attacaaagt gaaaggatac aagaaagaac aaaaagcagc acaaaaacaa attgaatcct
    31741 tccaagcaac cattgatttg aatgaagaat tagtgaagaa actaaaagat caattgcatt
    31801 atcaagttgc agacgatcag gaaaatactg aaaataagat taatagttac cagcaagaaa
    31861 ttgaaacatc gaaaacgcaa atgaaagatc aagaagagat cattcaaaaa cttcaagaaa
    31921 aaattgatcg tatgacaaaa gaaaatattt agaagaatag gtggagtaaa agaaatgaag
    31981 cgagcgaact tggtcggcaa agatactttc gctgagcaga aagtattgat taaagcctat
    32041 ctcaaagttg taaaagctaa agaaatatgt caagaagtgc atttatctaa cacaacaata
    32101 tctgagttga agaatggtag aaagagttta gataccttaa aattcagatc atttgaagct
    32161 ttgtatttag ctgctaaaaa atattttaca gctgataata aataaaaaat taaggtgggt
    32221 tctatgtgaa aaaaacgtat caaaagtttt tatggattag ctatatcgtc ctatttatcg
    32281 ttgggtttct cgtcagtgtg tccgtgatcg tcggaacgct ttttcttttg ttctctgacg
    32341 aagatctggt acttaagggg ttaatgagta cgttatcgtg tgtcatgtta ctttatttat
    32401 gtatacagat caacgaaatt atgccccctc attggcaaga aaagaagaaa gaatttaagg
    32461 aagaaatcga aataaaatcc aatgactgga ttgaatacac atggcttcct ctgaaaagaa
    32521 atagcaaacg aaaggaattt tgggcattgc aattatggcc aataggaaaa ctttttattt
    32581 atatggcttc acttatggtt actttaatcg gttatttggt ggtggtctat ggggtggcct
    32641 attacttgtt aagtgagcca aaacagatcg cggcgtcaaa tattttcacg gcagtaatga
    32701 cattaatgtg tgtcatgtgc ttttctattt ttagttactt actcgtccaa tttatcaggc
    32761 aaaatttaac aatgagtaaa cttttcaaaa gtttattgga cttacttatc ttaggcagta
    32821 tgttaacgac tctaacagat cggttcgaac aatctgaaaa aacggtagag gggataaaga
    32881 acttcttaac gacgattgat caatttataa atggatatat gtatttgttt gaagtaggct
    32941 ttattctcac tttgtttggt tgtgtattta taaaactcat taaaagatca atcgaaaaag
    33001 aaatggttat tgatctaatt ccgacgctga gcggctatga gtattacgtt gattttggaa
    33061 agtatgagga gttgatccat acggatatcg ttcacacgag tatccatgaa gtaaccgtta
    33121 agggcatcag atctacttta aaagatggac aggagacgaa aacaatcgac cgaagaaaag
    33181 aggtctacaa tttttataaa gttgaagcaa gtctacgata tggcattggg aaattgaggt
    33241 tagatgggag taatattcaa caaaacaaac catatgtttt aggaaaagtg gtgatttaga
    33301 acttcctatg aaagatgaat aaataaggag aatcttttga aaatgattct tctttacaga
    33361 attgtgctta tagttggcac ggagttaaaa atcttaaaga aggagcagtt attcaaagtg
    33421 aaagaacatc ataatcagtt actatgtatt aaaacgggta cgatgaacat tcaataccga
    33481 acagaatatt tagaattaac gaatcaaact gattttttgt cgattcgaga gcaacataag
    33541 caagaaaaat taatggctac ttatagccaa ttgattgatg agtgggaaca attcaaggaa
    33601 tctaaaacat aaaattccat ttaaacacct tattttttaa agagcataag gaaacgtgac
    33661 cccactttta aaaaaagtgg ttggataggc tgtgcctatc acgtttctga aaaaaataaa
    33721 tgaccccaaa ataccaatat ataggatagt acaaggatag caaaggatag taaaaggata
    33781 gaggtaggat agcgaaaagg ataggatcct tttcactatc ctatattata ttggaggata
    33841 tttatgaatg ttaaaattag agatttagat ccaaaattca tttctgaaat tgatcgaaga
    33901 tgcgttgaac taagtaatcg tacaggaaat aaatggagtc gtaatgatta tttaaagcta
    33961 ctcgttgaaa atgattttga tcgtcctttg atggaataca aacaagaaaa gtttgaccag
    34021 ttattagaaa agttttcaga aattcaaagt tataacacaa agatattaga agaatatgtt
    34081 agccaaaata atcgtattat agaaatcttg attgagcaaa atagaggaag tgaactttga
    34141 tatggcaaag gttatagaca aacacattta ctttcaccaa acacatatcg atgtattgga
    34201 tgaaattatt tctactcatg cagaggttaa aaactatagt gaagctgttc gctttttatg
    34261 tatgaactat gatccagaag tggagaaaaa agtaaattcc aatcaagcaa agttgaatgg
    34321 aatgagtaaa gaaatttcta ttttaaccga aatggtttca gaatttgcgg atcttcattt
    34381 gaaagataca ggaatattaa ttggtaaaaa ttcaactgtt tatcaagaag caaaagaacg
    34441 agtggaagga aaaattaaat ccaatcaaac aaaaaaatat tcccctcagt tgaagaaacc
    34501 tgtgaatgaa tctgttcatg ccagagtaga tccatttgat ccaacgaaga ttctaaaaag
    34561 ataaaaatga aggaggataa taatgactca atcgccttca gttattctca cttgtcaatt
    34621 tactttgccg aataaaaagt cattttctac ttatattgac tatattacaa gggaacaggc
    34681 tcttgaagaa aaggaacgac gtacgcccga agaagaacag gaacttcaac gaatcaagaa
    34741 tgctttagag gagttttata ttcctgaagg agaaactttt tcggagagta agacgggtaa
    34801 agaattcagt gaaacgtcga atgaagctca atcgttgatg gaacatggaa tcaattttga
    34861 caagttagag gaacaagatt ttaccaatta tctttcttat atgacacgtc actatgcgtt
    34921 agaacaaaag aaagaactga cgcaaagtga acaaaaagaa taccaacgct tgaaccaagc
    34981 aatgaaaaaa tatcaaacag aaccccataa aaaagcttca ggaaatcttc ctggagtttt
    35041 ttctatagct tcagacgaag tgaaaggaag tgatctgaag gagattaagc aaatcttcca
    35101 aagaggacaa caaaaaggct caatcattta tcaagatgtt gtttctcatg ataattcgta
    35161 tcttgaaaaa ataggattgt acgatcccaa aacagatact ttagatgaag aagggttaaa
    35221 agcagctgga agaaaaatga tggagacatt attcgagaaa gagcaactga acgaaacagg
    35281 ctactggatg gcaacgattc accgtaacac taaacatatc catattcatt ttgcagttgt
    35341 tgaaaaagaa aatacgcgta aaccttacac ggaggttgaa aatggtgtga gttatatcgt
    35401 accgaaaggt aagagaagac aagcaacatt agacgcaatg aagaccaatt atgtacatga
    35461 gttagaacgt ttttcttatg aaaaggatcg aatgattggt atggagaaac gaaatttatt
    35521 gacgcggaaa agtgatttgc gtaacacctt aacaaaagtg gtaaaagagc cagcgcgtta
    35581 cgatcgacac gcgatgaact tattaaatga agtatataaa accctacctg acaaacgttc
    35641 ggaatggaat tacggggatg aaaaacgaac caaactttca ccatacacaa gagagaaact
    35701 cgatgagtta accaaatatg ttttaacaaa agaacctgaa tatcaagaat atatacagat
    35761 ttcaaaagca ttgaaagaag aagcaaaaag tctttatggg gaaagcaaga gagaatcaaa
    35821 ggatgcagag aaaaatgctc tctttgattt aaaaaagcgc acaggaaatg ccatcctaac
    35881 agagctgaag agacaggata gtcaaataaa accattaata gaaagtgttg atcagatgca
    35941 atctatttac aatgaacgat cctttcgaac gcttaaacaa ttgccaacga atgaatacaa
    36001 atatagttac gaagaattca tgaagcgcag gaaacaattc caacaaagag ttgtcacgca
    36061 gcggagtttg cgtcaattaa atcgaacgat taatgcccat gaagaaaaat accacgcaag
    36121 taaagcatac gaagagcaac aacgaagaat tcaaatggaa caagaaagac agtaaagaca
    36181 gtaggaggaa tagcaatgaa acaatattta gatcaatgga aagttataga aggatcatta
    36241 agagaagaac gaatcgaaca acttcctgat tgtttagaaa aagagcattt gttccagata
    36301 agagaaatgt tgagaaacga acagtttgat ccaaatcaat ttttagtcgt tgagtaccca
    36361 gctactggtg tgtattgttg taatcatgtg aatggtgaaa aatattttat cattcaagaa
    36421 tatgagggca agttagcacc ttattatact acttgggaaa tgaatgaaga gggaatcaat
    36481 aactttccat gtaaaagcat tgaagagtcc atttcactca cagagtgtta gttgttaagt
    36541 gaataaaaga agtaaaagag tcctgaacgt gattgtccag gactcttttt ttcaatgaat
    36601 tgttttccgc caactcattg agtggtgcga tttgcgacta cattatatca aaagaaagag
    36661 aagggaggaa gcagaaatgg ttcattcaac gattggagtt gttgttgaaa aaagcagaga
    36721 caatctcgtt tttgtgacag aaattcaaac aggcagatct tttgtcgtca cagacaaagc
    36781 ggcaaaagct tatcaaagta gcgatatttt aacgttaaat ctgactacga agacgttcgt
    36841 cgatgcgaca gaagattatc catttgttta gtctaatggt gataagtcaa gatgaaattt
    36901 aagattttct ttattgagta atatgttttg aagcgcacga aactagaccc gatcaaaatc
    36961 aaattttttt cgggttgatt caaggatttg cgtcaataac ggattgttat ttctctatca
    37021 ca
//
LOCUS       DACGLH010000020        35719 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.022, whole genome shotgun sequence.
ACCESSION   DACGLH010000020 DACGLH010000000
VERSION     DACGLH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.022"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..705)
                     /locus_tag="IXT21_001124"
     CDS             complement(<1..705)
                     /locus_tag="IXT21_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868544.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
     gene            1393..1551
                     /locus_tag="IXT21_001125"
     CDS             1393..1551
                     /locus_tag="IXT21_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4868545.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            1623..2849
                     /locus_tag="IXT21_001126"
     CDS             1623..2849
                     /locus_tag="IXT21_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4868546.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="IXT21_001127"
     CDS             complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="IXT21_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ4868547.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="IXT21_001128"
     CDS             complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="IXT21_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ4868548.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4308..4775)
                     /locus_tag="IXT21_001129"
     CDS             complement(4308..4775)
                     /locus_tag="IXT21_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ4868549.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4895..6433)
                     /locus_tag="IXT21_001130"
     CDS             complement(4895..6433)
                     /locus_tag="IXT21_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ4868550.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6513..7199
                     /locus_tag="IXT21_001131"
     CDS             6513..7199
                     /locus_tag="IXT21_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ4868551.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="IXT21_001132"
     CDS             complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="IXT21_001132"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4868552.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="IXT21_001133"
     CDS             complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="IXT21_001133"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ4868553.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8685..9815)
                     /locus_tag="IXT21_001134"
     CDS             complement(8685..9815)
                     /locus_tag="IXT21_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ4868554.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9815..11998)
                     /locus_tag="IXT21_001135"
     CDS             complement(9815..11998)
                     /locus_tag="IXT21_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ4868555.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11995..13020)
                     /locus_tag="IXT21_001136"
     CDS             complement(11995..13020)
                     /locus_tag="IXT21_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4868556.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13065..13892)
                     /locus_tag="IXT21_001137"
     CDS             complement(13065..13892)
                     /locus_tag="IXT21_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ4868557.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13889..14956)
                     /locus_tag="IXT21_001138"
     CDS             complement(13889..14956)
                     /locus_tag="IXT21_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ4868558.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14982..15818)
                     /locus_tag="IXT21_001139"
     CDS             complement(14982..15818)
                     /locus_tag="IXT21_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4868559.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15815..16684)
                     /locus_tag="IXT21_001140"
     CDS             complement(15815..16684)
                     /locus_tag="IXT21_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4868560.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16754..18037)
                     /locus_tag="IXT21_001141"
     CDS             complement(16754..18037)
                     /locus_tag="IXT21_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4868561.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18051..19736)
                     /locus_tag="IXT21_001142"
     CDS             complement(18051..19736)
                     /locus_tag="IXT21_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ4868562.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20011..21036
                     /locus_tag="IXT21_001143"
     CDS             20011..21036
                     /locus_tag="IXT21_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4868563.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21631..25284)
                     /gene="rpoC"
                     /locus_tag="IXT21_001144"
     CDS             complement(21631..25284)
                     /gene="rpoC"
                     /locus_tag="IXT21_001144"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ4868564.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="IXT21_001145"
     CDS             complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="IXT21_001145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ4868565.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29708..30676
                     /locus_tag="IXT21_001146"
     CDS             29708..30676
                     /locus_tag="IXT21_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4868566.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30694..31599
                     /locus_tag="IXT21_001147"
     CDS             30694..31599
                     /locus_tag="IXT21_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868567.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31596..32402
                     /locus_tag="IXT21_001148"
     CDS             31596..32402
                     /locus_tag="IXT21_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4868568.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32559..33527
                     /locus_tag="IXT21_001149"
     CDS             32559..33527
                     /locus_tag="IXT21_001149"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ4868569.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33844..34368)
                     /locus_tag="IXT21_001150"
     CDS             complement(33844..34368)
                     /locus_tag="IXT21_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ4868570.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34655..>35719
                     /gene="scm"
                     /locus_tag="IXT21_001151"
     CDS             34655..>35719
                     /gene="scm"
                     /locus_tag="IXT21_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ4868571.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        10271 a         7540 c         6613 g        11295 t
ORIGIN      
        1 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
       61 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
      121 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
      181 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
      241 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
      301 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
      361 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
      421 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
      481 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
      541 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
      601 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
      661 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
      721 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
      781 ttttataatt tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact
      841 acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat
      901 gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttaaagtgga tagcgggttg
      961 aataagttga taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata
     1021 tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca
     1081 tgtttatcgt tattaatagt gaataggaaa aaacctattt ttgatcattg acaattaaat
     1141 acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg
     1201 attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat
     1261 agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct
     1321 atgtgcttac ccaaagcaat tcaaaaattc taatcttttt taggagatga ttttataaaa
     1381 ttgagagtct acttgaataa aaaacaaacc tgtgagtttc taggaattga taataatact
     1441 ttagataagt ggattcgtat atataatttt ccagtaatca gaattgacgg tatagttcgc
     1501 tttgacatag aggatatcaa agattttatg aatacattta aaacaatttg aaaatttctt
     1561 ctcgccgcat atactaagaa tagcgaaata tactgcctta ctttttaagg gagtgaggaa
     1621 ttttgcctac aaaactttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat
     1681 tcagagaaat cactggaatt acatcagaaa agtatcaaaa agtatttcca actaggcaat
     1741 tggctattaa agccgaaaat gcaatgaaaa ggaaaattga aacagtttta agagaggaga
     1801 atgcaaattc actagaatta aaaggaaaaa taaaatttaa ggaattctat gaatcaaaat
     1861 ggcttcctag atatgaacta ggtcagacta ctagatcaaa aagagctcca agttacgtaa
     1921 caattagtaa tactaaagat atattccgat tgcacatcct tccaatgttt ggtgagtatg
     1981 caatgaacta tctaaattca aatactgaga taatatcaga tgaattaaca aaaaaatcga
     2041 aagaatacgc aaatataaaa attatcaaag gatatgtccg ttctatgttt gatattgcag
     2101 aaatactaaa ttatattgaa tttaaccgaa ctaccaaaat tattcaatgt atcactgcac
     2161 ctaaaaaaat tgctttagaa gaaagaagga tacgggaagg taatcaagca ttatcgtcag
     2221 aagagcttat tgcttggata gaagcagtaa acactgactt gaataatcac ttgcttactc
     2281 ttcatgacta tactcttttc atgcttaccc tatatcttgg tgatagaaaa agtgagacgt
     2341 atgctttgca atggaaatat atcgactttg agaaacaaac ggttcgcctt aaacatgctt
     2401 tggataaata tcaaaaaaag aaatttacaa aaggtcgaaa ggataccgta attcaagtac
     2461 ccgaaattgt aatggcatta ctaagcaaat ggaaatcagt gcaagctgag caattgttga
     2521 gattaaaaat taatcaaaca ccagatcagt atctctttac ctatactaaa ccatccggag
     2581 aagtaaattg ccctgttcac gctgattacc taaactatcg aatcaattca attaaaagac
     2641 gacatcctga attggctcat ttatctccac ataagcttcg tcatacctat gcaactatag
     2701 ctagacaagg cggagcaaat atgaatcaaa tatctagtgc tttaacacac tcagatattt
     2761 ctacaacgag aatatacgta aatacaccag atgtagtaga taaaactgtt tttgaagcct
     2821 ttcagagagg attgaataaa tgtgattagt atgtgttctt aaatgtgttc ttaaacaaga
     2881 aaaagacact tttcaaacaa ttgaaaagtg ccttgaaaca ttgatatata aataatatta
     2941 acgtttagag aattgagatg ctttacgagc ctttttgaga cctggttttt tacgttcgac
     3001 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     3061 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     3121 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     3181 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     3241 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     3301 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     3361 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     3421 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     3481 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     3541 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     3601 cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt
     3661 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     3721 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     3781 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     3841 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     3901 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     3961 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     4021 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     4081 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     4141 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     4201 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     4261 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     4321 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     4381 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     4441 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     4501 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     4561 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     4621 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     4681 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     4741 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     4801 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     4861 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     4921 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     4981 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     5041 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     5101 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     5161 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     5221 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     5281 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     5341 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     5401 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     5461 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     5521 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     5581 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     5641 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     5701 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     5761 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     5821 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     5881 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     5941 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     6001 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     6061 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     6121 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     6181 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     6241 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     6301 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     6361 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     6421 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     6481 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     6541 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     6601 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     6661 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     6721 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     6781 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     6841 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     6901 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     6961 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     7021 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     7081 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     7141 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     7201 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     7261 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     7321 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     7381 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     7441 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     7501 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     7561 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     7621 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     7681 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
     7741 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
     7801 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
     7861 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
     7921 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
     7981 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
     8041 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
     8101 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
     8161 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
     8221 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
     8281 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
     8341 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
     8401 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
     8461 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
     8521 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
     8581 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
     8641 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
     8701 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
     8761 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
     8821 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
     8881 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
     8941 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
     9001 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     9061 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     9121 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     9181 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     9241 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     9301 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     9361 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     9421 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     9481 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     9541 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     9601 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     9661 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     9721 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     9781 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     9841 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
     9901 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
     9961 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    10021 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    10081 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    10141 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    10201 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    10261 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    10321 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    10381 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    10441 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    10501 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    10561 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    10621 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    10681 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    10741 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    10801 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    10861 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    10921 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    10981 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    11041 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    11101 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    11161 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    11221 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    11281 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    11341 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    11401 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    11461 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    11521 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    11581 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    11641 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    11701 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    11761 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    11821 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    11881 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    11941 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    12001 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    12061 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    12121 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    12181 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    12241 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    12301 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    12361 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    12421 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    12481 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    12541 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    12601 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    12661 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    12721 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    12781 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    12841 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    12901 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    12961 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    13021 ttatttctcc ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    13081 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    13141 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    13201 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    13261 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    13321 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    13381 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    13441 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    13501 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    13561 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    13621 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    13681 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    13741 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    13801 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    13861 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    13921 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    13981 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    14041 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    14101 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    14161 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    14221 ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    14281 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    14341 tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    14401 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    14461 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    14521 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    14581 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    14641 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    14701 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    14761 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    14821 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    14881 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    14941 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    15001 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    15061 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    15121 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    15181 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    15241 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    15301 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    15361 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    15421 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    15481 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    15541 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    15601 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    15661 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    15721 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    15781 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    15841 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    15901 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    15961 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    16021 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    16081 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    16141 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    16201 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    16261 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    16321 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    16381 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    16441 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    16501 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    16561 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    16621 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    16681 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    16741 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    16801 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    16861 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    16921 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    16981 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    17041 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    17101 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    17161 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    17221 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    17281 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    17341 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    17401 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    17461 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    17521 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    17581 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    17641 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    17701 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    17761 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    17821 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    17881 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    17941 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    18001 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    18061 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    18121 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    18181 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    18241 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    18301 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    18361 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    18421 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    18481 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    18541 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    18601 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    18661 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    18721 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    18781 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    18841 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    18901 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    18961 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    19021 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    19081 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    19141 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    19201 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    19261 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    19321 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    19381 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    19441 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    19501 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    19561 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    19621 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    19681 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    19741 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    19801 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    19861 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    19921 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    19981 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    20041 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    20101 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    20161 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    20221 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    20281 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    20341 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    20401 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    20461 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    20521 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    20581 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    20641 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    20701 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    20761 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    20821 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    20881 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    20941 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    21001 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    21061 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    21121 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    21181 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    21241 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    21301 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    21361 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    21421 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    21481 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    21541 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    21601 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    21661 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    21721 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    21781 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    21841 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    21901 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    21961 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    22021 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    22081 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    22141 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    22201 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    22261 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    22321 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    22381 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    22441 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    22501 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    22561 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    22621 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    22681 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    22741 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    22801 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    22861 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    22921 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    22981 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    23041 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    23101 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    23161 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    23221 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    23281 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    23341 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    23401 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    23461 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    23521 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    23581 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    23641 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    23701 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    23761 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    23821 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    23881 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    23941 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    24001 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    24061 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    24121 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    24181 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    24241 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    24301 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    24361 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    24421 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    24481 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    24541 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    24601 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    24661 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    24721 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    24781 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    24841 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    24901 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    24961 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    25021 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    25081 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    25141 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    25201 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    25261 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    25321 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    25381 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    25441 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    25501 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    25561 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    25621 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    25681 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    25741 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    25801 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    25861 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    25921 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    25981 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    26041 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    26101 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    26161 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    26221 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    26281 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    26341 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    26401 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    26461 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    26521 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    26581 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    26641 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    26701 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    26761 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    26821 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    26881 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    26941 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    27001 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    27061 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    27121 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    27181 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    27241 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    27301 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    27361 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    27421 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    27481 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    27541 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    27601 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    27661 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    27721 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    27781 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    27841 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    27901 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    27961 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    28021 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    28081 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    28141 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    28201 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    28261 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    28321 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    28381 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    28441 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    28501 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    28561 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    28621 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    28681 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    28741 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    28801 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    28861 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    28921 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    28981 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    29041 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    29101 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    29161 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    29221 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    29281 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    29341 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    29401 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    29461 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    29521 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    29581 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    29641 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    29701 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    29761 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    29821 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    29881 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    29941 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    30001 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    30061 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    30121 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    30181 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    30241 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    30301 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    30361 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    30421 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    30481 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    30541 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    30601 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    30661 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    30721 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    30781 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    30841 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    30901 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    30961 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    31021 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    31081 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    31141 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    31201 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    31261 atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct
    31321 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    31381 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    31441 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    31501 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    31561 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    31621 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    31681 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    31741 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    31801 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    31861 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    31921 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    31981 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    32041 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    32101 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    32161 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    32221 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    32281 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    32341 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    32401 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    32461 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    32521 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    32581 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    32641 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    32701 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    32761 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    32821 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    32881 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    32941 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    33001 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    33061 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    33121 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    33181 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    33241 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    33301 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    33361 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    33421 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    33481 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    33541 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    33601 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    33661 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    33721 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    33781 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    33841 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    33901 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    33961 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    34021 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    34081 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    34141 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    34201 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    34261 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    34321 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    34381 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    34441 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    34501 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    34561 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    34621 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    34681 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    34741 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    34801 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    34861 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    34921 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    34981 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    35041 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    35101 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    35161 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    35221 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    35281 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    35341 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    35401 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    35461 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    35521 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    35581 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    35641 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    35701 tgatagctca gattcatca
//
LOCUS       DACGLH010000021        35280 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.023, whole genome shotgun sequence.
ACCESSION   DACGLH010000021 DACGLH010000000
VERSION     DACGLH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.023"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="IXT21_001152"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="IXT21_001152"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ4868572.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="IXT21_001153"
     CDS             1013..2260
                     /locus_tag="IXT21_001153"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ4868573.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="IXT21_001154"
     CDS             2336..2680
                     /locus_tag="IXT21_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868574.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="IXT21_001155"
     CDS             complement(2797..3123)
                     /locus_tag="IXT21_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868575.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4697)
                     /locus_tag="IXT21_001156"
                     /pseudo
     CDS             complement(3496..4697)
                     /locus_tag="IXT21_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4814..5329
                     /locus_tag="IXT21_001157"
     CDS             4814..5329
                     /locus_tag="IXT21_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4868576.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5528..5974)
                     /locus_tag="IXT21_001158"
     CDS             complement(5528..5974)
                     /locus_tag="IXT21_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ4868577.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6021..7499)
                     /gene="msr"
                     /locus_tag="IXT21_001159"
     CDS             complement(6021..7499)
                     /gene="msr"
                     /locus_tag="IXT21_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4868578.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7853..8482)
                     /locus_tag="IXT21_001160"
     CDS             complement(7853..8482)
                     /locus_tag="IXT21_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ4868579.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8466..8930)
                     /locus_tag="IXT21_001161"
     CDS             complement(8466..8930)
                     /locus_tag="IXT21_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4868580.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9417..10685)
                     /locus_tag="IXT21_001162"
     CDS             complement(9417..10685)
                     /locus_tag="IXT21_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ4868581.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10916..11653)
                     /locus_tag="IXT21_001163"
     CDS             complement(10916..11653)
                     /locus_tag="IXT21_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4868582.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11720..12289)
                     /locus_tag="IXT21_001164"
     CDS             complement(11720..12289)
                     /locus_tag="IXT21_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ4868583.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12417..13289
                     /locus_tag="IXT21_001165"
     CDS             12417..13289
                     /locus_tag="IXT21_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4868584.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13631..14503
                     /locus_tag="IXT21_001166"
     CDS             13631..14503
                     /locus_tag="IXT21_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4868585.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14500..16458
                     /locus_tag="IXT21_001167"
     CDS             14500..16458
                     /locus_tag="IXT21_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4868586.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16709..17560)
                     /locus_tag="IXT21_001168"
     CDS             complement(16709..17560)
                     /locus_tag="IXT21_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4868587.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17683..18150
                     /locus_tag="IXT21_001169"
     CDS             17683..18150
                     /locus_tag="IXT21_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ4868588.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18413..18895)
                     /locus_tag="IXT21_001170"
     CDS             complement(18413..18895)
                     /locus_tag="IXT21_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4868589.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18995..19858)
                     /locus_tag="IXT21_001171"
     CDS             complement(18995..19858)
                     /locus_tag="IXT21_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4868590.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20456..21304
                     /locus_tag="IXT21_001172"
     CDS             20456..21304
                     /locus_tag="IXT21_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868591.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21475..21816)
                     /locus_tag="IXT21_001173"
     CDS             complement(21475..21816)
                     /locus_tag="IXT21_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ4868592.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21902..22609
                     /locus_tag="IXT21_001174"
     CDS             21902..22609
                     /locus_tag="IXT21_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ4868593.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22596..22919
                     /locus_tag="IXT21_001175"
     CDS             22596..22919
                     /locus_tag="IXT21_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ4868594.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23008..24045)
                     /locus_tag="IXT21_001176"
     CDS             complement(23008..24045)
                     /locus_tag="IXT21_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4868595.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24492..25475)
                     /locus_tag="IXT21_001177"
     CDS             complement(24492..25475)
                     /locus_tag="IXT21_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ4868596.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25891..26661)
                     /locus_tag="IXT21_001178"
     CDS             complement(25891..26661)
                     /locus_tag="IXT21_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868597.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26654..27589)
                     /locus_tag="IXT21_001179"
     CDS             complement(26654..27589)
                     /locus_tag="IXT21_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868598.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28183..29832)
                     /locus_tag="IXT21_001180"
     CDS             complement(28183..29832)
                     /locus_tag="IXT21_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ4868599.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30027..30923)
                     /locus_tag="IXT21_001181"
     CDS             complement(30027..30923)
                     /locus_tag="IXT21_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4868600.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30916..31596)
                     /locus_tag="IXT21_001182"
     CDS             complement(30916..31596)
                     /locus_tag="IXT21_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4868601.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31597..32379)
                     /locus_tag="IXT21_001183"
     CDS             complement(31597..32379)
                     /locus_tag="IXT21_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4868602.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32372..33289)
                     /locus_tag="IXT21_001184"
     CDS             complement(32372..33289)
                     /locus_tag="IXT21_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4868603.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33292..33576)
                     /locus_tag="IXT21_001185"
     CDS             complement(33292..33576)
                     /locus_tag="IXT21_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4868604.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            34364..>35280
                     /gene="ecbA"
                     /locus_tag="IXT21_001186"
     CDS             34364..>35280
                     /gene="ecbA"
                     /locus_tag="IXT21_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family collagen-binding adhesin EcbA"
                     /protein_id="HAQ4868605.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKT"
BASE COUNT        10588 a         7342 c         6324 g        11026 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttc
     4441 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
     4501 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
     4561 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
     4621 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
     4681 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
     4741 aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
     4801 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
     4861 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
     4921 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
     4981 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
     5041 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
     5101 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
     5161 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
     5221 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
     5281 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
     5341 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
     5401 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
     5461 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
     5521 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
     5581 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
     5641 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
     5701 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
     5761 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
     5821 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
     5881 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
     5941 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
     6001 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
     6061 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
     6121 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
     6181 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
     6241 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
     6301 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
     6361 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
     6421 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
     6481 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
     6541 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
     6601 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
     6661 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
     6721 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
     6781 ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
     6841 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
     6901 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
     6961 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
     7021 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
     7081 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
     7141 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
     7201 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
     7261 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
     7321 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
     7381 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
     7441 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
     7501 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
     7561 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
     7621 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
     7681 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
     7741 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
     7801 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
     7861 acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
     7921 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
     7981 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
     8041 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
     8101 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
     8161 gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg taacattgca
     8221 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
     8281 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
     8341 gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
     8401 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
     8461 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
     8521 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
     8581 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc gattttcttc
     8641 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
     8701 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
     8761 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
     8821 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
     8881 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
     8941 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
     9001 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
     9061 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
     9121 attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt tacaccgcca
     9181 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
     9241 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
     9301 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
     9361 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
     9421 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
     9481 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
     9541 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
     9601 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
     9661 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
     9721 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
     9781 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
     9841 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
     9901 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
     9961 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    10021 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    10081 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    10141 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    10201 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    10261 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    10321 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    10381 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    10441 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    10501 attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt ttcaattcct
    10561 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    10621 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    10681 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    10741 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat aaatttgctt
    10801 tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    10861 gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt atggattata
    10921 cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct ggcgtttcta
    10981 tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct ttttgcgctt
    11041 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    11101 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    11161 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    11221 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    11281 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    11341 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    11401 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    11461 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    11521 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    11581 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    11641 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    11701 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    11761 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    11821 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    11881 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    11941 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    12001 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    12061 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    12121 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    12181 ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag agcggatccg
    12241 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    12301 cttttatcct tactttataa cggacggctt atcatgtcta atacttataa aacatactta
    12361 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    12421 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    12481 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    12541 aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg gattctggtc
    12601 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    12661 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    12721 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    12781 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    12841 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    12901 aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa accgtcatcc
    12961 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    13021 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    13081 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    13141 ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg ccattacaag
    13201 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    13261 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    13321 taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt tcttaattta
    13381 tttcttcctt ttatttctga taattccagt aaattactat aattataaaa aaagaattga
    13441 caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta
    13501 gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt
    13561 ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa
    13621 gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg
    13681 tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt
    13741 gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt
    13801 gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga
    13861 tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga
    13921 ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt
    13981 aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta
    14041 tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca
    14101 tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat
    14161 tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc
    14221 attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca
    14281 tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc
    14341 agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc
    14401 gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat
    14461 cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga
    14521 atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg
    14581 atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc
    14641 ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg
    14701 tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta
    14761 aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat
    14821 ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca
    14881 gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc
    14941 aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg
    15001 ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta
    15061 ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg
    15121 catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa
    15181 tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca
    15241 atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa
    15301 aacaaagcaa aaacagcttc cttgatgcta gcagcagctt ttgcttcatt ctttactggt
    15361 gtgacagaac cacttgaatt ttcatttatg ttcgttgctt ggccattata tgtcttgcat
    15421 gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt
    15481 gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag
    15541 ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac
    15601 tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat
    15661 gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac
    15721 gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt
    15781 ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct
    15841 ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc
    15901 gttgcagatg agatggcaaa actacatggt ggtgctgcag cacgacctgc acaaacaaac
    15961 accgtggtta aaacagaaac atttgccggt gaaacgatcg aacaagatat ttacgctatc
    16021 gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg
    16081 atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt
    16141 acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc
    16201 gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta
    16261 tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc
    16321 gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa
    16381 gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct
    16441 gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg
    16501 agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc
    16561 aactccgcgg taataagtcc agctaaacga aacatatgaa gagctatttt ttatttgact
    16621 cttcttatta cttgatgcag aacagctttt tcacaaaatt tttcttcata ctcaaaacta
    16681 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    16741 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    16801 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    16861 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    16921 ctttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    16981 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    17041 attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    17101 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    17161 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    17221 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    17281 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    17341 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    17401 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    17461 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    17521 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    17581 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    17641 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    17701 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    17761 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    17821 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    17881 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    17941 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    18001 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    18061 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    18121 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    18181 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    18241 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    18301 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    18361 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    18421 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    18481 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    18541 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgcttacaa
    18601 caagatacgt tccttttggg aatgggatga ttcgcgtagt gtttggaaca tcctgactcg
    18661 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    18721 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    18781 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    18841 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    18901 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    18961 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    19021 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    19081 cttcgactaa cccataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    19141 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    19201 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    19261 gcatcaaact gcccttttct tccaagcggt ctccatcaaa tccataagaa acgttgatta
    19321 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    19381 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    19441 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    19501 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctactga
    19561 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    19621 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctagaaga tcgcgattag
    19681 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    19741 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    19801 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    19861 ttatcttcct ccttgattgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    19921 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    19981 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    20041 ataaattttg gggaagacag attctaagta aacaaagcgg taaaaatatc ttttattccc
    20101 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    20161 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    20221 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    20281 tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa
    20341 tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt
    20401 cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga actagatgaa
    20461 taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct atcatctgca
    20521 cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag tttctggcac
    20581 ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt gtgattgtct
    20641 atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg cttacgaaga
    20701 acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg tttcctccgc
    20761 tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc cgcactttat
    20821 cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata aattaacaac
    20881 tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa cagcgaagtt
    20941 catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgcttattc agcaaaatgc
    21001 ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa cattctattg
    21061 ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt tatggaaaga
    21121 attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt tcgtctgcca
    21181 agtcgatcat actgacgcct cttattcaaa acgctactct ataaaaatcc tgaaagaaga
    21241 ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaac taaagaaacg
    21301 gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg ataacaacca tcctaaatcc
    21361 acaagaaata cagcagacct tgctttaaaa acacgaatca tgcaaaggat gaagtaaaga
    21421 agacttaacg cgtacccaat attagtcatt tgactattag gtacgcgttt tgttttataa
    21481 gtcatgacaa atttccttcg ttgctggata gattttccgg cattgttgat agattttatg
    21541 atacttttct acattttgta agagaggttc cgctgcatct gtatattgga taaaattctg
    21601 acaacaactt tcaggtgtgt cgaaccaccc taaccctagt gcagcaatca ttgttgcgcc
    21661 taatgcaggt ccttcctcca cacttaaaca atagaccgtt gcatcgaaaa tatcagcttg
    21721 gatttgtagc catgcaagat ttttcgcgcc tcctcccacc gaaacaatac gtgtaaatct
    21781 gcgacccgct agttctttac gtttcaatcc caccattgac aaatgctcat taaaaattta
    21841 gaatattcac aagtttaacg acaattatga aaatttatgt cgtgctaaag gagttttgtt
    21901 attggatgaa aagttagata taaaaactgc gatcaaagac acgttaccta ccgttttcgg
    21961 ttatatcggt attggacttg catttggtat cgttgggaaa gctgccggat ttcatccact
    22021 agtcgtcacg ttgatgtccc tgctggtcta tgctggttct gcccaattta tcacagtcag
    22081 catgcttgct agtcacagcc cattgctttc catcgttttc tcgacctttc tagtcaattc
    22141 ccgaatgatt ctgatgagta tgacgattgc tccttatttc aagaaaaatc ggctgcttca
    22201 aaatctgctg attggcacat tgctgacaga tgaaagtttc gcactgggaa tgaataaatt
    22261 gaactatact ggacaaaagt taaactttcg ttggatgaat acagctaatt ggatttctta
    22321 tttgacgtgg gtgggttctt ctcttgttgg cgcactttta gggaatttta ttactgatcc
    22381 gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt atcgggttac tttatcttca
    22441 agtcatttct gatagaaaca cctctaaacg attacagttg atcctgattg gtctcacttt
    22501 gattctggta tatgtcggac tgatttttat cccaagcaac ttagtgattg tagttgtaac
    22561 actgattggg tgcggattgg gggtgtggat caaacatgcc ttcttttgag tttacattgc
    22621 tgactatttt aggttgtact ttagcaactt ggctttctag ggtcctacca tttgtcttac
    22681 tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt aagttttgtc ccgatcgtca
    22741 ttatgtctgc tttatggttc agcagtctat ttacccaaaa tatcggacat ctgccgcaga
    22801 tcaatctcga aaatgctttg gcttcagttc ctacactttt agctgctatt ctttcaaaaa
    22861 gcttattggt gatcgtcttg gcaggaattc tttccctttc gttgattcgc ttattataaa
    22921 taaaaaaaca agagcttatg gcaaattact gtcatgcgct cttgttttta tcaaagtcaa
    22981 ataagaaaaa aatcggcgta ttcttgctta tccgttgaac ggaccactaa caacgacttt
    23041 tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt ttcaagttct tcgcattcat
    23101 cccatctgaa tagaccttgc tcaatgtact gcggataatc ccttcatttg ctaaagcagc
    23161 aatgtgcgct aaagcttctc cttgcgtttc gatttcatag ttatagtctg ttttagcgaa
    23221 catatactcc cagtcgaagc tgatagattg gtttttccaa acacctatat ctaacggctc
    23281 tttgatccct acgatcgttc ccacatgtcc aaatggtcgg acaaggtgct tgatttgatc
    23341 aaagtagcgg gtaatatcga ataatacagc gacatactct acttgatcga ttcctaattc
    23401 acgaaaactt ttacttaagt cgtgatgata atcgattgga tagtctacac ctgttttttt
    23461 cagccattca aaattcttcg gacttgcagt cgcgtagact tctaggccag cccaatgcgc
    23521 tagttgattc aaaatcgaac cgacgccccc ggatccgttg atcactaaga tttttttccc
    23581 gcgattggca ttttcttctg gagttagacc aaatttttca aataacaatt cataagcggt
    23641 caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa agattttttg gtgcaagagc
    23701 gacaatacgt tcgtcaacta gttggtattc ttgattgctt cctgctcttg ttgttgtacc
    23761 tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta cagcaacaac
    23821 tttcccgact ccatcaaaac ctaagatacg taaagcattt cttatcccgt tatcttggcg
    23881 aagtttcgta tcgacaggat taacagatac tgcgttgact tttaccagaa gatcccgtga
    23941 actgggtaca ggtatctgtt ctttttcatc aataaagctt tttggatcat cgatcggcaa
    24001 cccttcataa aaaccgacta ccttcattaa ttccttttct gacatacgaa ttcctcctta
    24061 gcttcatcaa gttggagcat ccatacagtg ttcatttcaa tatatatagt atgctatttg
    24121 ttccaccttt tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt
    24181 ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttataaa cggtgttctc
    24241 ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac atgagaagct gttttttgaa
    24301 attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctgatat aaacggtgtt
    24361 caatcaactg accctcggta ttagctcaaa aaacgacaaa atatgaagag ctatttttgt
    24421 ttggctgttc ttattacttg atacaggaca gctttttcac gacctctaat tcacggtgtt
    24481 caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtctctaag
    24541 atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct
    24601 cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg
    24661 atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct
    24721 ttttctgtat gttccctcgg gtctttttca aatgcataag gattcttgaa aatccctcgg
    24781 ccaatcatga tgccatcaac gccatatttc tcggccaatt cttctcctgt ctggcgatcg
    24841 gggatatctc cattgatcgt gattagtgtt tgtggagcga tttggtcacg taaagcaatg
    24901 attttaggaa tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa
    24961 tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca
    25021 gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagct
    25081 gcttcaatca attgtgccgc tacttctgga cgaaggatca agccactgcc ttttccacgt
    25141 tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct
    25201 gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc
    25261 atcggctgtt catcttctgt aaatgttaat cttcctcgta cactgtcttt tccatctgga
    25321 tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc
    25381 tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa
    25441 ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca
    25501 agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga
    25561 agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaaagact gtgacaaaaa
    25621 tcttatcaca gtccattttc cggaataaat ggtgttcaaa agcttgcatt tgcggtaata
    25681 agtccaacca aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat
    25741 gcagaacagc tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta
    25801 ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc gtttgtttct ctaatactca
    25861 ggtcaaaacg ctgatttcac aacctctttt ctaacttctc aatcctcttc ctttattgat
    25921 caaataccaa gtaacggcgt acaaaacaac gatgaataac acaagtacga tcatcgatgt
    25981 tgctactggc acatcgatcg ttccaaggaa tccgtaacgg aaaccggaga tcatataaac
    26041 gatcggattg acttttgaaa cagcttgcca aaagggcggt aacatagaga tggcatagaa
    26101 aacgccacct agataagtta acggctgcaa gacaaatgta ggcacgatcg atacatcatc
    26161 ataagactga gcaaaaatcc cgttgagcag tcctgctaat gaaaacaaga ttgccgtcat
    26221 caataatgta atgatgacca tgaaccatga atacacatga agtggtacaa agaacaagga
    26281 aatgatcgtt acgagagagc cgacgagaat actgcgaccc acaccaccaa tcacaaagcc
    26341 ccaaataatc acatgcgtag gaacaggtgc taccaagatt tcttcgatat ttttctgaaa
    26401 tttttgagag aagaaggacg aagaaacatt ggcatacgaa ctagtgatag cagacatcat
    26461 aatcaaacca ggaacgatga attccatata tgaaaaacca cccatgtcac cgatccgccc
    26521 accgatcatt tttccgaaaa taacaaaata caaggatgtc gtgatcactg gcggaaccag
    26581 tgtttggacc catatacgca aatagcggtt cgtttccttg atagctaaac tttttaatgc
    26641 agtaaaataa agattaaaca tttttctcct ccgtatgatt attttcttct gtgattttca
    26701 agaacagttc ttctaaacga ttcgacttat tcctcataga aagaactttg attccttgtc
    26761 ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg acagcgattg
    26821 tctgctcatc ttcaaatgta caagcatatc cttcaatcac aggtttggta tcatatggcg
    26881 ctaaatcaaa gataaatgtt tcaaattgca gttttgacag aagctctttc atacttgtat
    26941 tctcgatcag ttcacccgat tggatgattc cgatgttgcg acaaagcatt tcagcttctt
    27001 ctagataatg cgtcgtcaaa atgatcgttg taccattttc gttcaattct cgtagaaact
    27061 cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt tggttcatcc aagatcagca
    27121 aacgtggttc atgcatcaat gccctagcaa tcatcagtct tcgcttcatt cccccggaaa
    27181 gcatacgtgc acggacattt ctcttttccc aaagattgga ctgcttcaaa tatttttcgc
    27241 tgcgtttcat cgcttctttg cgagagacac cgtagtagcc tgcttgattc acgacgattt
    27301 gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg aaccaatcca atctgttgct
    27361 ttgctcgaac tagatccttg tccagatcat aaccgaagac attcactttt ccagatgttt
    27421 tattgactaa agaagtaata atcccgatcg ttgtagactt cccagctcca tttggcccta
    27481 atagcgcata gaaatctcct tctttcaccg aaagatcgat tccccgcaat gcttcgaccc
    27541 cacttccata gatctttttc aaattattta tttcaagcgc atttgccatt tatttcctat
    27601 ctccttatag acatgattct taattgtatc atggaataca gcagatgaaa aaacaacttg
    27661 gcttccacac aaaaacacac ctgctaacaa agaggagatg tgttttctag ttcatgatca
    27721 tcttcgatag aataactatc taaacaactc tggaactttt aaaatcataa aagtaccgac
    27781 agcaactact acgatgacat atagaagatc cttataactg aggttcttgt cttttttatt
    27841 catgttaagc tttcctccgt ccttttatcg aaagcttttt tcttttattt taaccgaaat
    27901 aaataaaatg gaaatagtta ttttcaattt ctgttttcgc tgtttgtttt acggagaact
    27961 ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt ttcgataaaa gctgctagaa
    28021 ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa tcttccaaga tcatctggac
    28081 agcagactcg ttcccattga attctgccac ttttttggca tcaatgtact tcttttgata
    28141 atcgtctttt tggacccacg catcatgctt cgtttgataa agttaatcaa acggatacca
    28201 ctgacgctgt tcatcgaaca acaaactttc tttttgggcc tgttcaacta tggatttagg
    28261 ataattcaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa
    28321 tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc
    28381 acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga
    28441 tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt
    28501 tgttcctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc
    28561 cagcaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc
    28621 atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat
    28681 cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga
    28741 ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga ctggattaac
    28801 taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa
    28861 ctctggtcgg caagtgatcg gcatataggt gcgataattc aaaacggctg ctgcaacttc
    28921 caattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt
    28981 gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat
    29041 caattctgct tcactcccgc tcttcattcg aaatgaaaaa cctaatttag ggatgtctct
    29101 taatggttta gtttgctgtt tcagctcttt ttgaagtggt gtcgtcattt ttgctaactg
    29161 attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt
    29221 ttgtttcttc actagataat agaccgtatt gaatactaag ccaccgattg ctagatagat
    29281 tcctagtact tgccctaaaa gaagcaaacc tacacccacc agcggaagca agccaagaaa
    29341 aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc
    29401 aaaattatga tctttttttc ctaaacgagc gaattgatat tggattcttt ctctcagttg
    29461 aggattttcc tgataaaaag caattagttt ttctaactga taatcttctc caaaatcaaa
    29521 gctacgtaaa cgctgataca acgcttctga acctacacta gaataggtac tgttgatccc
    29581 ttcaaatact tcgaacatat ccaagtcata ccaagtaaga tcgtcgattt cactatccca
    29641 tgaacggaat tttttctccg tgagccacgc atccttcaaa ctttcttctt tatcaaatct
    29701 cggttgataa ggaatttttc cccaattact acgaactgtt tcctttactt tcaaacgtga
    29761 aaaaatgtct aaaacaacta ataaaataac aactgcaata ataattaaaa cgataaattc
    29821 tctttggccc atatccctct ccgttccttg atttgttagt tttagtatag cactgttgcg
    29881 ctatttgaaa agggggtaca aaaataactg tcttcgttac ctaattcgta tcaatcacac
    29941 caaaataaaa acaccttcca aaaaagaaga tgtctttaaa acgagataca attttttgtc
    30001 attcacttca ggtaaattag agccaattaa accctccgca acttgacgga gatggagaag
    30061 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
    30121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
    30181 cgctggaaga ctttttcaac agaggtttgc gctcctggtt tgataggatt ggccactgtt
    30241 aatcgaatga atgaatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
    30301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
    30361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
    30421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
    30481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
    30541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
    30601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
    30661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
    30721 tcattttcat ccacccatgt attcagtgca tctactaggt ccattagatt cttttctcga
    30781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
    30841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
    30901 agaagcaaca gccacttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
    30961 agcatgtgcc tttgctagtt tccctcgaag attggaaata tgcatattca ctgttttctc
    31021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
    31081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
    31141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
    31201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
    31261 taacaacagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
    31321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
    31381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
    31441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
    31501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaagaatgaa cagcttcatc
    31561 gtcttcgata atcaaaatag tttccagcag gaacacctac ttaatgtcag atttttggaa
    31621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
    31681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
    31741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaacgaaggc
    31801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
    31861 tgtcaaagcc actggaacag ataatgtcgc taatatcagc agaagcaata caggaaatac
    31921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
    31981 tgtgtaagca atcatggcta gccccgtaat caaaaccacg ccaagtgttg tgatcaaaag
    32041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
    32101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
    32161 cagaagaatc catgcagcca ttttttccac cattaaaatc cctgttgcac cgttgatgac
    32221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
    32281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
    32341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
    32401 gtaagaaata atcttccagt ttttgcccaa ctaggtggat accatccaca ggaacttttg
    32461 cctctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
    32521 cacggctaga ttggacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
    32581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
    32641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc gtagtgagtg gcgatctgtg
    32701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
    32761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
    32821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
    32881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
    32941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
    33001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
    33061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
    33121 aacccataat agttctcata aaggtagact tacccgctcc attctcccca atcaaaccat
    33181 agatcattcc acgtttgatt tcgatcgata ctttatctaa tgctttggca gcaccgtatt
    33241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
    33301 tttcttattg ataagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
    33361 aagaaagtgt aaattttcca attgaatttc aagaaaaatc agactgtctc gcagacgtgg
    33421 ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct aatccaagtg gactggccac
    33481 catggtaatc gttgttatcg ttgacatcag acgtccttgt atatgcgttg gaacttctcg
    33541 ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac ggacttatga
    33601 aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt tatgacaaaa
    33661 gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc ctcggtcaca
    33721 agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt atttcttgaa
    33781 gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga tgatacttta
    33841 tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc tacctattaa
    33901 catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag aaaagttagg
    33961 attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac tcgtgaactc
    34021 gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt tcttaagacc
    34081 tggttttttc ttcgtatcat tgaataagtt tgaaaaacct tgatttataa aggttttccc
    34141 gtaaatatat tttattatat ttgattaaat ttgaaatgtt tgtgttctaa aatgtgttct
    34201 aatttaaagg cagtatattt cgtattttag attaaatttt taggctcatt cattctcaaa
    34261 tccattctat tcagtcaata aaaattttaa aaagacttta taaacaaaga tgttcaagcg
    34321 agcatcttat ttttttgtgc ttaatttgaa aggagaacat ttcatgtata aaaaacaaaa
    34381 aaagaaatta tgtgtattaa ctattttgag tttggctgtt caagcaattg ttggagcaat
    34441 tgctcctacg attacttttg ctgatgaaat tacccatcca caaacggtaa ccgtagaact
    34501 agatttagca catcaatatg ctgttgaagg aacttttagt gatggtcgtc caatgtcaga
    34561 agtcactgtt ccgcattatg cagtgtacaa cggctttaaa caagatattt tctgtattga
    34621 accaggagtc ccgatttaca atgagttcac tcctggctat gagaaaaacc cgttaccaga
    34681 tatgtccgat aaagccaaat tagtttccgt tttatggaaa aaagtaggta cagatgcaga
    34741 tactcatatt gtggcacaga agatgatctg gcaagaagtt aatgggtata cccttcactc
    34801 aataaaacga tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa aaataaatca
    34861 agccattgac gattatcaga agaaaccaac cttccataat agtacggcta aaacagtatt
    34921 agggcaatcg actactgtga cggatacgaa taatctgaat ttgtcagagt tcgatgaggt
    34981 tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt aatcagttga taatcacccc
    35041 aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag aaatctgctg gtaccggaac
    35101 acctgtcgcc tataaaaagg ctggccaaca gactttaatg gccggggcga ttgataaacc
    35161 taatacctac acaatcaaaa ttgatgtgga aacggagggt tctttaaaga tcaaaaaagt
    35221 cgataaagaa tcgggtgatg ttgtaccagg aacggttttc catttagact ttggaaaaac
//
LOCUS       DACGLH010000022        35061 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.024, whole genome shotgun sequence.
ACCESSION   DACGLH010000022 DACGLH010000000
VERSION     DACGLH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.024"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..1447)
                     /locus_tag="IXT21_001187"
     CDS             complement(<1..1447)
                     /locus_tag="IXT21_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4868606.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLK"
     gene            complement(1437..2732)
                     /locus_tag="IXT21_001188"
     CDS             complement(1437..2732)
                     /locus_tag="IXT21_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4868607.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(3064..3741)
                     /locus_tag="IXT21_001189"
     CDS             complement(3064..3741)
                     /locus_tag="IXT21_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ4868608.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(3771..4862)
                     /locus_tag="IXT21_001190"
     CDS             complement(3771..4862)
                     /locus_tag="IXT21_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ4868609.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(4963..5397)
                     /locus_tag="IXT21_001191"
     CDS             complement(4963..5397)
                     /locus_tag="IXT21_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4868610.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(5981..7438)
                     /locus_tag="IXT21_001192"
     CDS             complement(5981..7438)
                     /locus_tag="IXT21_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4868611.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(7435..9330)
                     /locus_tag="IXT21_001193"
     CDS             complement(7435..9330)
                     /locus_tag="IXT21_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4868612.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(9388..11163)
                     /locus_tag="IXT21_001194"
     CDS             complement(9388..11163)
                     /locus_tag="IXT21_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4868613.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(11213..12709)
                     /locus_tag="IXT21_001195"
     CDS             complement(11213..12709)
                     /locus_tag="IXT21_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4868614.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(12903..14525)
                     /locus_tag="IXT21_001196"
     CDS             complement(12903..14525)
                     /locus_tag="IXT21_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ4868615.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(14975..16288)
                     /locus_tag="IXT21_001197"
     CDS             complement(14975..16288)
                     /locus_tag="IXT21_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ4868616.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(16533..16757)
                     /locus_tag="IXT21_001198"
     CDS             complement(16533..16757)
                     /locus_tag="IXT21_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868617.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(16777..17145)
                     /locus_tag="IXT21_001199"
     CDS             complement(16777..17145)
                     /locus_tag="IXT21_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4868618.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(17341..18051)
                     /locus_tag="IXT21_001200"
     CDS             complement(17341..18051)
                     /locus_tag="IXT21_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868619.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(18069..18565)
                     /locus_tag="IXT21_001201"
                     /pseudo
     CDS             complement(18069..18565)
                     /locus_tag="IXT21_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(18602..19054)
                     /locus_tag="IXT21_001202"
     CDS             complement(18602..19054)
                     /locus_tag="IXT21_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4868620.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(19189..19794)
                     /locus_tag="IXT21_001203"
     CDS             complement(19189..19794)
                     /locus_tag="IXT21_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ4868621.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(20244..20864)
                     /locus_tag="IXT21_001204"
     CDS             complement(20244..20864)
                     /locus_tag="IXT21_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4868622.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(20866..21735)
                     /locus_tag="IXT21_001205"
     CDS             complement(20866..21735)
                     /locus_tag="IXT21_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ4868623.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(21758..22165)
                     /locus_tag="IXT21_001206"
     CDS             complement(21758..22165)
                     /locus_tag="IXT21_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ4868624.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGINYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(22184..23473)
                     /locus_tag="IXT21_001207"
     CDS             complement(22184..23473)
                     /locus_tag="IXT21_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4868625.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(23500..23802)
                     /locus_tag="IXT21_001208"
     CDS             complement(23500..23802)
                     /locus_tag="IXT21_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4868626.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(23827..25920)
                     /locus_tag="IXT21_001209"
     CDS             complement(23827..25920)
                     /locus_tag="IXT21_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4868627.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(26409..27137)
                     /locus_tag="IXT21_001210"
     CDS             complement(26409..27137)
                     /locus_tag="IXT21_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ4868628.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(27283..29904)
                     /locus_tag="IXT21_001211"
                     /pseudo
     CDS             complement(27283..29904)
                     /locus_tag="IXT21_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(30018..31349)
                     /locus_tag="IXT21_001212"
     CDS             complement(30018..31349)
                     /locus_tag="IXT21_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4868629.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(31315..32793)
                     /locus_tag="IXT21_001213"
     CDS             complement(31315..32793)
                     /locus_tag="IXT21_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4868630.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(33098..34933)
                     /gene="tdc"
                     /locus_tag="IXT21_001214"
     CDS             complement(33098..34933)
                     /gene="tdc"
                     /locus_tag="IXT21_001214"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ4868631.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        10138 a         7499 c         5797 g        11627 t
ORIGIN      
        1 ttttcagcgt ctctattctt cttttcaatc gaataatgtc gtaaggtgac gcaaattctg
       61 acagataata ttcacactgt tttttgtttc tctggcaaaa tgtgatcacc aaatcatatt
      121 ctttaccttc ttcataactt ccaatctcaa taccgccgat tcccctcagt tctctgatca
      181 gatattgttg aaaagcaata cgataaatcg gcagactttc taaatcgatt ccaatcacta
      241 ctggttttgc aatataaaaa tcgatcatca aaagaacatt ggcatactgg atcatcaggt
      301 aactaagctc actatcttca gctcttttcc tcggcaattg ctgcaccgct gtttcctgta
      361 atttcgccag taactgtgtt aaggaatgcc cgagcatttt ctgctgctgt tccaaaagcc
      421 gatcacgctc atagacttcg atctttccaa caaagaaaaa ccattcattg ttgatctggg
      481 aaatttggta gcctactgcc ttttcttctt tgattttcaa acgacgagga cgataatgcc
      541 ataacatcgt ttcccgaatg taagtatcta aaacagcggt tggtaacttt ttgctccgtg
      601 tcaaatcata ctgataaaat atttcttcgt ctactatcga aaaagaaaca aaaaagctgt
      661 aaaacatcat tgtttcgtat acattcagtt ctgctgctgt tcgactaaga tgcatcacga
      721 tgattggatc gattgcttga taaagccggt ccgactgata gagtaatttc ttttctttca
      781 aagttgcgta cgtacttttt tcattcaata gtcttttttt cgtaatcccg agccagcaag
      841 caatctgtgc ttctgctgat gccgtaaacg ttgtttctaa tacacggtcc aatcctagaa
      901 tgaacggtct tttttctggt gtattttgca aaaaaagcgg tcgctgatct tccgggatat
      961 attggaatag ttcataataa aaatatcgta tctgtagctc ttctccgtat aattgaccgt
     1021 ttttgatttg taatccaaat tcctctaaga gctggttcaa ttctttgatc tttcgaaaaa
     1081 cagaagactc actgatatta aacgctgtcg ctaatcgaac gatggatact tgctgatgtt
     1141 ctaataaata gacgagcatc ttaaacttca agctttcctg caataaaaac aatagaattt
     1201 tttccaaact tacatcttcg tccaacacaa gcagcgcctg atactcattt cgctgtactt
     1261 ctactttttt ttccatcatt cggccaatca attcaatatc ttccaaatat tgatcaaacg
     1321 aggcttttga cagttggaca tgctctctca aaccattgat agaggctgtt cccccttgca
     1381 gaacaaggtt tcgcaaaatc aggatctgta actgctcttt tctttctaat aatgcttcaa
     1441 tcttcatagg cagtattcgc cacctttgac cacaccatat ctggcacacg gtattctggc
     1501 tggaaaaata gctcatgttc ttctaactgt tcaaacgtcc atccttcttt tactgcaatt
     1561 gtcagttggt caagaaggtg gaacatctct tttttgcttt ggagctgtgc tccaagaatc
     1621 tttttttcag aatcaaatag aaatttgagt ttaactttgc tttgatttat tgctgtaagc
     1681 ttgtagtcta cgtatcctgt ttcaattttt tggggataaa aaatgctctc ttcttctgtc
     1741 aaacctacgc ttcctaagaa atagccaaac cactgattgc cgactggacg ataggttccc
     1801 tgatcctttg tctgtgagcc actaattgtc ttgctgacat ttcgtgctgt acggacagcg
     1861 ttatttacta gagaaacata cattttttca tttgttggac gaaaagttac ttggatcgca
     1921 tcgccaatcg cataaacgtc tttttgagac gtttgcaagt agtcatctac ccagatcgtt
     1981 ccatcatcat tcaatgttaa ttgttcttcc cacatctgat tgtcggggcg agaatgatta
     2041 gccagcaaaa caagatcata gttctccttt cttttttccg tctctacaat agcggtttca
     2101 ttttcggtca aagacacaac ttgttcttct aaaaaaagtg aaagcccttg atttttcatt
     2161 tcctttacta acggttctgt catttcagga tctagataac gaaaaagaat tgactttcgg
     2221 ctttcgtaaa gatggatttg tttttttctt gatgccaacc cttcagctat ttcgattcct
     2281 gcttgtcctg caccaataat tgcaattttc tttgcattca tgatcttttg ctgaagagcc
     2341 gttacgtttt gtatcttttt aactgggtga ataagcgttg attctgcaga aatatttcga
     2401 aacttttgat ttgaaccagt cgctagaatc aaacgatcaa aatccaattt ttttcctcct
     2461 gctaaaacga cttttttttc tttcaatcct attactgtct tttctgtgta aacatggatt
     2521 cgatagcggt cagtcagttc ttctttcgtg atccaagaaa gttgtttttc actggtaacc
     2581 tttcctttta atatcaaatt gaacccgctg ggaatccatc ctaacttcga ttgcttttca
     2641 atcaaatata tttcacttgt tggcatcaat tggcgaagat aaatagccgc atgaatccct
     2701 gcaaacgacc cgccgacaat tacaatgttc atctgcattc ctctttctta taaggtgttc
     2761 tactgctaac ttctcaatca gaaaacacag tattcataac tcgctttcaa ccttatgata
     2821 catagcgatg cagcagaatc aaccctctcc atcataccgt gttctaggtc tgcttctcaa
     2881 taatgtacta gtggacctaa ttgaagagta tcatttctga agagatgttt caaaaaatgt
     2941 actcttataa aagaaaaaaa ctcgcctttt acgtacctct gtccaaatag gaaacagaaa
     3001 agaacgaaaa gacgagtttt agcgtgtgta atgtaatagt tagaatagac acatcattta
     3061 tttttaattc tttaatgctt caacaactgc ttcgcctaat tgatgagcag attgaggatt
     3121 ttgccctgta agaagccgcc catcaatttg aaccacttct gaaaaagctg ctccagactg
     3181 atagtttgct cctttttctt tcaaagcatc ttctgctaag aatggtactt cattcgttgt
     3241 accatttgct tcctcttctt cattactgaa cccagtcact gtacgtccag ctaaaataga
     3301 ttttccagac tcatctgtaa ctggcaacaa tcctacaaca ccgtgacaaa cagctgagac
     3361 cagcccttca tttcgataga tttgtgaagc gactttccca agatctattg cttctggaaa
     3421 atcccacatt gttccgtggc caccagcata ataaaccgct gcataatcat ttgcatccac
     3481 atcatcagga cgtaaggtat ttcccaaggc ccgatttctg aaatcttcat cttcataata
     3541 ggcccaatcg atttcatcca tattcaaaac actttgcggt tctaaaggta catatccgcc
     3601 tttaggactg acaaaatctg catcgatccc attttttact aatacatcat aaaaatgagt
     3661 cagttcactt aaccacaaac ctgtaggacg ctcgatcttt tcgtactttt ctacatttgt
     3721 taatacgatc aatactttca ttttcccatc ctctttctat aaaaattgcg ttattgataa
     3781 cgtgctttca agaaattata taatgggctt ggcaaataaa gctctttata tgcttcttca
     3841 aaaggtttcg ttacaaattc atcctcacgg ttttccaata atttctctgc ccagtcagga
     3901 tcgacgatca tttgctgtcc aattgctgca agttctgcat atttcaatac ctgctctaca
     3961 tcttcgcgat tgcggatccc accgacacca actaaaggga tctttccttg aatcgtgtta
     4021 tggaccactt caagaatcga tttatcgttg tatttatcag aacgagctat tcgatcatac
     4081 atatttaatg aaacatgcag ataatctagt tttgactcac gtaatcgttc aagaagccag
     4141 atcgtgtctt caaaacggat acctggtgtt tcatattctt ctggtgagaa acgataccct
     4201 acgatgaatg gttttttagc atattgttca accgctgaga aaaccgttgt taataattct
     4261 tcaataaaac gataacgatt ttccaagcta ccgccccatt gatcctctcg tctattcgaa
     4321 tgcggagaga aaaattgttg gatcaaatac gtatttgctc catgcaactc gattccatca
     4381 aatcctgatt caatcgctct acgtgtggct ttgccaaatg cttggattgt ttcaacgatt
     4441 tcttcttcac tcattgctcg tggtgtttcc gcatcaggac gttttgctgc aactgcactt
     4501 gcagatacag gctgttctcc agataaagtt gctcgacttg tcattcgtcc accgtggaaa
     4561 atctgaataa tcgcttttgc gccttctcct tggattgctt tcgctagctc acttaatcga
     4621 ggaatcattt catcatgagc gatgctgagt tcacctggcc agccttttcc gatatcttgg
     4681 acatttgcag caccagtaat aacagcacca agacctttag aacgttgttt ataataagtg
     4741 atctcatctt cggtgatcac tccattgaaa aagctgagtt gtgtcgtcat aggcgacagc
     4801 atcaatcggt tcttcaattg aactcctgaa ggtaaagtaa ttgattgatc aaattgtgac
     4861 attgtcattc ctcccataat tcgctctgat taacctatcg agaatcatct atcattaaac
     4921 cactcgatta gttgtacatg caactaattt gcttaaaaaa ttttattttt tttgtaactt
     4981 atctgttgca tcattcattg tctgggctaa ttctttactt gcttcatttc ccaaaactgc
     5041 tttgtatgac tgatgaagtt cttcccagct accatcgact tccttagcaa attggcgtcc
     5101 ttgttctgta agataaacta atgacaagcg accttcgcta actgttttga cgtatccttt
     5161 atattctaat ttttcaacaa aacgagcaat cgttgatggg gcggaataga gcatttgtgc
     5221 aatatctttt tgtggtattc catctttttc ttttaatatc aataaaataa aaccataagt
     5281 tggtgccaga cctgtccttc taaacgcttc atcagctaat ttattcaaca tgcgatctag
     5341 ctttttcacg ctaaagaaaa gacattcatc taacgctttt tcaattggct tttccatttc
     5401 atcccctcca ttggcgtaca tatcatagta tgatttttat tttttgtcaa aaaacacgga
     5461 ataatgtccg cctcctctgt ctagatttga aaaaaagaag tagccatctt caaagaattt
     5521 cggcttgttt tcgacaaaac tccttctgga acaccgtgaa tcagaggttg tgaaaaagtc
     5581 gtttagtttt gagtatgaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa
     5641 tttggcttaa taccgaaaaa ctggcttttg aacaccgttt atatcagagg ttgtgaaaaa
     5701 gccgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat attttgtcgt
     5761 tttttgagct aatgccgatg gtcagctgat tgtacaccgt ttattgagag gtcgtgagcc
     5821 tgccgttgaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca tgttttatga
     5881 ctatggtgac ttgtgatcga ggagttggca ggagaatact gtttattcgt aaagaagggg
     5941 ctgtgacaag acttttgtca cagtccctta tttcctttta ttataggatt tcgccatttg
     6001 attggatgac ttgtctgtac caatcaaatg attttttctt taaccgtttc tttgtgccat
     6061 tcccttcgtc atcttgatca acatagatat atccataacg ttttgacatt tgtgaagtag
     6121 aagcgctgac gatatcaatg cagccccacg acgtataacc aaataaatct actccatctt
     6181 cgatagcttc cttcatctgc tcaatatgct gcttcaaata gtcaatgcgg taatcgtctt
     6241 gaacggttcc atcttcttct actttgtcta tagctccgag tccgttttca gctatgaaca
     6301 gtggtttttg atacctatca tataattgat tcaaagcaat tctcaaaccc ttaggatcaa
     6361 tctgccagcc ccattcactt gatggaagat acggattttt aattcctcca gttattaagt
     6421 ttccttggac cccttccaaa ttttctggat gagcactgac tgcactagac atgtagtagc
     6481 taaaagaaac aaaatcaaca gggtaggctg ctaagatttc ttcatcacct acttctattt
     6541 ttaagttgac acctttttct tcaaacattc gttgcgtata cgctggataa tatcctcttg
     6601 cttgaacatc actaaagaac aaagaagttc tttgctgctc aattgttttt tgaatatcat
     6661 ccggatcaca agaatacgga acaagaagct gatagctgat catacaaccg acttgtgaac
     6721 ctttgataat ttcatgacaa gcttttgtag ctaaagcact tgctataaac tgatggtgtg
     6781 caccttggta tttcgcttgt tccaaatggg gatggttttc ttcaattact cctacgctaa
     6841 caaaagggtg atgttttaca cagttgatct cattgaaagt catccaatat tttactttat
     6901 ttttatagcg tttgaatact gtttcagcga aacgaacgta aaactgaata agctttcgat
     6961 taggccatcc accatattct gtaaccaaat ataacggcat ttcataatgg gaaagagaaa
     7021 cgactggctc aatcccatat tttagacatt cgtcaaacac ttgatcataa aaaaataatc
     7081 ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat tcttgtccat gcgatagaca
     7141 tccgatagca tttaaaaccc atttcagcaa ataaacggat gtcttcttta aagtgatgat
     7201 agaaatcatt tccgtgtctt ttaggataat aatacttgct gttgggatcc tgcgccgctt
     7261 ttacttgctg atgcgtcatc atatgccatt gatctgtcca tttttcttta ggtaaatcgc
     7321 tgtcgaatgt atacacatca gacacagata atcctttttc atctacatcc catgcacctt
     7381 cacattgatt ggccgctgtc gctccgcccc ataaaaatcc ttctggaaat tttttcatat
     7441 cgatccccct aaacttgcta gttttcttgt ttgattattt ttcttatgac tagtaatgat
     7501 atttttgaat ctactactat ttgtaacaat aaccattgtg gtcggatcat atccctcttt
     7561 gattaattct tgcaaatcta cttccatcaa taaatcgcct ttacgtactt tttttccatc
     7621 agatactgca ctttgaaagt atttgccatt taaattcact gtatcaatac cgatatggat
     7681 cagtatctct gctccttctt cactgactac cccaattgca tgtttcgttg gaaaaactgt
     7741 tgaaacttcc ccgtttacag gactgtagat ttttccgtcc gttggaatga tcgcaaaccc
     7801 ttctcctaaa gatccactag caaatgttgg atcgtttact tcagataaat caattatttc
     7861 acctgctgca ggagtactta acactatatc ctttgaggat tcttcttcga tttcatcctt
     7921 gtagccgaaa agaaaagtta gcatacagga actgatcatc gctaagaaat aactagcaac
     7981 tagccaaaca aatccttcac caatataaat cgggaaagac aaaatacttc ttgtagcaac
     8041 tgccaaagca ccggctcctg cggcaccagc tactccacca aatatagctc cacatataca
     8101 agcaatgtaa aatggtcgtt tatatttaag agtcacacca taaacagcag gttcagtgat
     8161 tccaaataat gctgtgataa atgcagataa agataaactt tttactttct tatttttcgt
     8221 tttcaaaaat accccaagta ctgctcctgc ttgcgcagct acggcagtca tacatgctgg
     8281 acctagcgta tcacgaccgt agaaagaaag attgttggtc attactggta caagtcccca
     8341 atgtaaccca aaaatcacta acacttgcca aaatccgccg ataacagcac cagataaaac
     8401 agggctaaag ttataaacag ctaaatatcc agctgcaatc agctgacttg cataatctgc
     8461 aattggacct acaactaata aagtaattgg gaccatcacg atgatacaaa tgaatggtgt
     8521 caataaattt cgaatacttt cgtgcaagct tttgcgaatc ttaggctcaa tataagaaag
     8581 aacccataca gctaaaattg ctggaataac cgtacttgta tatcttgcta gaatcacggg
     8641 catccctaga aatcgcaagg ttatgctatc actatatgca ctgacaatcg taggataaac
     8701 gagagctgct gcaataacca tagcgatgaa acgatctgta ttgaactttt ttgcagcagt
     8761 atatgccaaa aatataggca agaaatagaa aaatgcatca gctgctgcat tcaaaatgat
     8821 ataagttcct gattcagtag ttaataaatt caaactcgta cataaggcga gtactccttt
     8881 gatcaaacca cttcctgcca agacgcctaa agtcggggca aaaacagcgg cgatcatgtc
     8941 tataaaagag tttaaaatgc cttttttctc agttcctctc tttccactag atgcatcatc
     9001 ttctagtttc atttgtttgg ataatacgtc atacacttca tttactgcat ttcctactac
     9061 gacttgaaac attcctccag cttcgactac agtgatcaca ccttctagtt gctctacctt
     9121 ctccttatct gccttcttat tatcttttag ggtaaagcgt aaccttgttg cacaatggta
     9181 aacattgctg acattttctt cgcctccgac aaatcggaca atatctttcg ctaattgtga
     9241 atagtccatt tttacttccc ccttgttaat taatgccgta gcaatccttc ttacaagata
     9301 agtatagttc ttagaaaacg gtttctccat tcaattagta ccccttctaa tagcgaacga
     9361 aactgtacaa tagtgtgtat ggtttactta gccgagcaaa tcactcactg cttgaaagaa
     9421 aacaagtata tcgtttctct tatagtcaaa ggagacaaaa aaaacacgct tgatcttcct
     9481 ttgctgatgt ataagtggta tctcaagctg atactctgtg atttttgttt ctacttggct
     9541 tttgataaga cagcacaaca aagtctcggc atttaacgga agcataacta ggtcagaact
     9601 aggagagatt agttgctgaa tggaatctat ctttttatgg atttttattt gattttcttc
     9661 tttgtagtaa tgatcaagca gttttgtttt ctttttttct tcaactatgg tcagcttttc
     9721 acgcaaaatc tgataaatct ccgaagattc aaactcatct agaacactag gtagataaat
     9781 caaatcatcc cactcaaaaa taagctcttg ctcagtagtc aaaattgcat catattccgt
     9841 tattttgttt gattgatttt tgaaaagata cactggttct atccggcatt ctttgatcca
     9901 catgttcata ctttgttgaa tctgtttttt tagtgtattc aaaatactaa ttggctggtt
     9961 actaacaatc agtatctccc cttgttttcg aaaaggattc aacgcttcgg caagatacaa
    10021 aaccaaataa gcgctttcag ctaaattggg acgtaattga aaatgtattg ggaagaaacg
    10081 ccgaattaaa taaatttcta aaggataacg ggagatgatc ttttgtacaa aatgattcat
    10141 ggtattatgt ccggcatcca tcctcgtttt catttgtttg atatgggtta gcaaaaattc
    10201 tgtttcatgg aacaataacg aaacaggaag atctaaaaaa cgaataatcg tttgctcaaa
    10261 tgctcttaga ttcttctcta tttctttgtt tttttcatca ttaatcatca aataatttaa
    10321 ctctaatagt cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat agggtagttc
    10381 tgatgataac ttttttgtga gggtctctac catcggaata aataatgtat acctgtttgt
    10441 tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg agataccgag aaaatcgtat
    10501 gaagtcctct cctgaaacaa tataattata tgatataaga acctctttta atgtcttttg
    10561 gacagtcggc atgatattgg aaaaaattgt actgatttgt tgaggcaacc ttgcgtgttg
    10621 gatcaccgtt tctgttccaa caagagataa gaaaattctt tttgaatttt cttcagcaat
    10681 gatttttagt ccttttgtcg acgaaacttc cagttctaat ttaggattgc gctcgatcca
    10741 acgcaaaatc gtttttattt gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc
    10801 tatcttttgc atactgacat accctttttg aaacaataag tacgtcacaa tagttgaagc
    10861 tcggaaatcc atgctggaat agattttatg tttcgtcacc tcttgtatga tttgctgttt
    10921 agtttcagat tcaattgatt ctaaaaaata acctgaacgt ttatttgact ggataatgat
    10981 tttcttctcg tagatagcaa gcatattttt gatttcagtt tggatcgttt tcttgttggt
    11041 acctaaagct tcagctaatt cttcgcttgt tatccaatga ttcttatttc ttaatactac
    11101 aagaatttgg agttgtcgtt tatttaagtc catttgctta cacctcctct ttgttataaa
    11161 catacaggaa aaacctctca aatgacaata gtcacttgca gaggtttaaa aatcatttga
    11221 agaaagatct atattcaagg tttttaccat tagactggat aatttgttta taatattcaa
    11281 aactatcttt tttcaatctc ctaaagcttc cgctcccatc attttgtcta tccacataaa
    11341 taaagccata tcgcttcctc atttcccctg ttcctgccga tacgatatcg attggtcccc
    11401 accataagta accaatgatt tggcaaccat cttttattgc ctcagcaatt tcctgaagat
    11461 gtatttttag atactcacaa cgttttggat cgtggattcg attcaattca tctggtgctt
    11521 catctagtcc aatgccattt tcaactatga ataatggtaa ttcataacgg tctgtcaatt
    11581 cattcatgac ataacgaaac cctagaggat caattggcca actccaaggt tcgggagatt
    11641 tttccttcaa aaagggatta tcgaaaccat ttgctccgcc agtatctacg cgcatttctg
    11701 cttctttttt atagacagcg ctacgataat aactaaatgc aatataatct accgtgttag
    11761 cttgcaagta ctgttcgtct ccatctaaca ttactggtgc acagtcttct tccttccata
    11821 tcctgtaaat ataagctgga taatgccctc ggcacatgac atcggagaaa aaccatgaat
    11881 tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc acagtcatat gcataagtcg
    11941 ctaatgcaga aaagctacac ataataccca tcttagcatt tggatcgatt ttctttaata
    12001 attgagtagc acgggcgttc gctagaaaca tatgatgaca ggcttgataa atctcttttt
    12061 ttgttagtgt agcaatgggt tcaagtttat ctctagcagg gaatgtaacc aatccagcag
    12121 ctgcataagg catacgaaaa gcattattga tctcattaaa ggtcatccaa tatttcactt
    12181 tcccttggta gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg attaattttc
    12241 gattttgcca ccccccatac tctcctacca atgctaaggg ggtttcataa tgactaagag
    12301 taattactgg ctcgatatca tatcgattta atgtattgat tagtctgtca taaaatacga
    12361 gaccagcctc atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc
    12421 ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg aatatcctct tcaaatcgat
    12481 gataaaaatc aatcgcttca tgagaaagat aatggagtga ttcgtctaaa gcaatctccc
    12541 aaatcttttt ttcttcattc cactgaatac taggatgctg gttcatgatc ccgataagcg
    12601 tatctgtcac attaggtaat ttgccgtctt ccaaccatgc accttcacat tgattagcag
    12661 ctactgctcc gccccataaa aaattttctg gaaatatttc ctgatccata actatctctc
    12721 ctcgctatca acaatttctt acttgaagta tagctcgatt catcaaaaca agatattcta
    12781 actatttcgg cagaagagaa gaaaaaaatt gaatgctact cttcattttt aatatttata
    12841 tataacaaat acataagacg atccccgaaa gaaaacactc gttaaccatc taagcaaacg
    12901 atttatttta cttccaacac aaatgcttca taaggtcgta aatcaacttt gccgtctggt
    12961 aactgtttca gaggataatt tgacacaata acccttaaag cttcacgtgg aacagtgaac
    13021 gtctgctttt cactggacat attacaaata acgagccatt gctggtcttc atatatgcga
    13081 gaataggcaa agacatgctc aggcgtcttt tccactaatt catagtctcc ccagacgatg
    13141 atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac agaatcttga
    13201 tctgctaacg cttcttctgc attgatttct ttataatttg gattgactcg aagccatgga
    13261 attccagtag taaacccagc atgttcacgg gcgttccatt gcataggcgt cctagcattg
    13321 tctcttcctt tcgcattgat tgattccaag atttcctctt ttgtaaatcc agaagaaatc
    13381 ctttcgttat acatattgat tgtctcaata tcttttgctt ccgcgatatc atcgattggg
    13441 caattcgtca tgcctaactc ttctccttga tagatataag gggtgccttt catcatatgg
    13501 agcaagatag caaacatctt ggcactttct tttcgataca catcgtcatt tccccaacga
    13561 gaaacaattc gcggaagatc gtgattattc caaaataaac tattccatcc ttcatcacct
    13621 aaagatgttt gccatttgga taatactttt ttcagttcag agatactcaa gggtttcaaa
    13681 tcccatttgt ctttgccttc ttgctgatct aatcctatgt gttcgaattg gaagaccatg
    13741 gataattcat ttcttttagg attggaataa agtttagcga tttcggtagt tgctccccat
    13801 gtttcgccaa cagtcatcag atttttgtca ccaaaagttg cttgattcat ttcctgaaga
    13861 tactcatgca gtttaggtcc attacttgtg atttctttat ccggttcttt gccaatcata
    13921 tcgatcacgt ccatacgaaa gccaccaata cctttatcga tccagaaatt catcatttga
    13981 taaatctctt ggcgaacctg tttatttttc caattcaaat caggttgacg cttgctatac
    14041 aagtgcagaa aatattgccc cgttttttca tccaactgcc aagcagaacc actgaatatt
    14101 gagcgcaatc tattaggctc ttttccattg accggatcac gccaaatata atagtcccga
    14161 taaggatttt ctttattttt acgggcctca atgaaccacg gatgttcatc agaagtatga
    14221 ttcaccacca gatccatgat gatacggata tttcttttct ttgcttcttc aatcagttct
    14281 tccatttctt ccatcgtacc gaattctgcc atgattgctt gataattact gatatcatac
    14341 ccattgtcat cattcggaga ctcgtatact ggagaaagcc aaattgcatc aatacctaaa
    14401 tacgccagat aatccaacct ggaaataatc ccccgcaaat ctccaatacc atcttgattt
    14461 gaatcttgaa aacttcttgg atagatttga taaacaactg aatggtgcca ccatttttgt
    14521 tccatgattt tcctcctata aatggatgtt tctatttttt gcttaatcat ttattttagt
    14581 ctctctcact aaaaattgta acgctttcaa atagctattg caccttgtta tcggtaacag
    14641 aataaaacaa aaaaacagct gaggaaattc cctcaactgt tttttgaaag gattgattta
    14701 ttcagggaag aatgctgatt tcacaacttc tttataaacg gtgttcactc agctgtccct
    14761 cggtattagc tcaaaaaacg gcaaaatata agaaactatt tttttcgttt ttttctctaa
    14821 tactcaggcc agaacactga tttcacaacc tctttataaa cggtgttcac tcagctgtcc
    14881 cttggtatta gctcaaaaaa aggcaaaata tgaggaacta ttttttcgtt tttttctcta
    14941 atactcaggc cagaacactg atttcacaac ctctttattt cacattcaat atccacatga
    15001 atcccatgaa tacaaagaac agaccataca tgatgggaga tacttctttt cctcttttag
    15061 ctgctaacat ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat
    15121 aagtcaaagg catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc
    15181 attcgatttg tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag
    15241 ttacttgtga agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa
    15301 tccctgtagt aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt
    15361 ctacaaatgc accaacagga gaagtaccta ataaggaacc cgcaagcata gctgttgaat
    15421 cagaagctaa agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt
    15481 ttgctaatcc aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca
    15541 ctacgaccca catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg
    15601 ttggtttcaa acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga
    15661 ccagacctaa aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa
    15721 caagcaagat agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga
    15781 gtgatcctaa tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac
    15841 caaggaaagc aataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga
    15901 tcgcatcaat gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag
    15961 caacgaatac gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat
    16021 aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata
    16081 cccccattaa aatacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt
    16141 catccattcc agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga
    16201 taaatgttgt aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt
    16261 cgaagtagga aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat
    16321 aaagtccgtt aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca
    16381 aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa
    16441 atccctttat ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg
    16501 ttaataaagt ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt
    16561 cgtttctgtt cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg
    16621 ccaaaacctg tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga
    16681 ttctttgctc gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat
    16741 cttcccatta gatccactgt ttctcctcct ttttattcaa tcagacgact cagcttttcc
    16801 tataacaaaa taattgcctt cagggtccga aaaattgaaa gtcaagacgc cattgttgtc
    16861 tgtgatttct cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa
    16921 tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg
    16981 gaatacgcct aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg
    17041 caattcgatt gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat
    17101 ctctacgtca ttgacataaa gcatcacacg tgttttttgt atcatcgttt gacccttctt
    17161 tctatttgat tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca
    17221 ccttgtgttt gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc
    17281 acaaactttt tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt
    17341 ttataattct gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa
    17401 tgtttgatcg attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc
    17461 agcattgatg gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct
    17521 ttgggcttca cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat
    17581 ttcttccgga agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgtcacc
    17641 ttgggctttt cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg
    17701 tacgccaaaa gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa
    17761 ttcccgttct tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt
    17821 agtcttgaat gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt
    17881 ttcttctgta aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt
    17941 ttcttcactt tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt
    18001 tgcttcagga aatacttccc agaaattggt atccactatt acttttttca tctgttcaca
    18061 cctcttgatt agaataattg cttgatgaat agatagtctg tttggggatc atctcccata
    18121 taaaaaacat gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca
    18181 tcgttgtgtt cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca
    18241 atagctgtat ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag
    18301 attcgctcga tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt
    18361 agtttcaggt aacccgcagg ctcttgatcg atactcacta ggaaaaaatc tgtttcttta
    18421 tcttttagct cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa
    18481 tgctccggta attatctttt ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta
    18541 gtaagtctac atctgaaggc gtcatttttc gaatttcgta tttattcacg gcttcttcct
    18601 ttcaatagat ccttttattg ccttttttga caaaattcca atcttcgcta acattcttgc
    18661 atactttatt taaaagatat tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg
    18721 cgactgcatt ggagtagttg ttttctcgaa tgatgaaagg atagatttct gctccttttt
    18781 tagtcggata gagtttcttg atttttttat tttctttatc ttccaaacgt tcgatcatcc
    18841 cattggattc taatttcttg attgctcggg cagtcgttgt acgatctact ttgatcattt
    18901 ctgctagttt ttcttggatg attccaggat tttcgcaaat acgtactaaa taaagatact
    18961 gtcctcttgt taaatcgact tctttgaatt cgatattgct aatagagtct agcgctcggg
    19021 caatcattcc gatgtctcga aggatttcag ccatttcatc ccacctttat tttgtttgta
    19081 agactagttt acctcttttt gttgcaaatg caactatttt taataaaaaa atcaagttct
    19141 tttttaataa ctagtaaagt atattctttt cttcttttac gaaaaagctc aatcacaata
    19201 gatctggttt ttgttttcta aaataagtaa ctgtttcttc gttgttcgct tttctgatag
    19261 ctttggcaac tcttctatac taaaaaatcc catgttggaa gtttcattat tctcaataaa
    19321 cttggcctct tcaccgtgga tcgcacaagc aaaaacaagt ttataatact gaaaaagctg
    19381 tggaatatct ttttgtttat tcgtatcaaa aactgctcgc aactgagtag tttctactgt
    19441 taatcctgtt tcttcataga cttctttacg cacattttct tcaggagaca atcctatttc
    19501 agcaaatcca ccaggaagtg accattcttt cgtccgttta tcttcgatta gtaggacctt
    19561 gccatctttt ttgatatagg cacgaacatc tacttttggt gttggatgtc cttctccaat
    19621 cgttgtgagc gcttttattt cttcgaaagt ttcttttcct atttcactga tcagttccaa
    19681 tgaaatcgtc cggatctcct caaaacgctc tttatcaaat acatcttttc cataatatag
    19741 accagcttga gaaagtgcga gtagtctttt gtattgagcc aacttcttgt tcattctctt
    19801 cccttctttt ttagcaaatg tatgactatg taatcaagta tagaagactg gtttaattac
    19861 ggcaaataaa tataatgaaa tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa
    19921 aagacattcg acttctctgg ggctgtgaat aaatggcgtt caaaagcttg catctgcggt
    19981 aataagcccc atcaaacgaa aaatatgacg agctattctt gtctggctgt tcttattaat
    20041 tgaggcagga cagctttttc acaacctccc cactaaacgg tgttctccta ccaactcctc
    20101 ggtcacaagt cacaatattc atcaaacatg agaagctgtt tctttgaaat tgctccttgt
    20161 gactcggagc tgaacgacag gctcacgacc tctcggtaaa cggtgttcaa aagccaattt
    20221 ttcgatatta agccatttct ttgttactta ttgtcattga tttctttttc taatttatcc
    20281 aatagtggag tgtaatccac ttgagtatag tctgttacat caaattcata tagtgttcct
    20341 aactggaatt tgtcatattt tcggtcggcg atgatctttt tgaatgcttc tttcgtaata
    20401 tgattggtcc ctttcgttcg atcttccaat acgtcaccat agtgataatg atccatgata
    20461 tagctcaaat gacgatctga ttcacgatca cgtttgtacc gacgttgcca taacacttca
    20521 aagtctgctg ttaggcgaat cgtgatcacc tgatatccgt atctatctgc taattcttgg
    20581 agtcgtgctt tttgcttatc actaaaagga tactcggaca caatcacttt ctttcctgct
    20641 tccatataca aatcaagtgc tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc
    20701 gcccgttctt ccaaattgtt aaacccgatg gattctgcat agtattcctt gatttcatcg
    20761 agcgtgatgg catacatatc tggaaatcgt tcccgtagtt tattcataag atatgttttt
    20821 cctgtaccag gactaccagc cagaagaatg aaatactttt tcatctcatt tatttcctaa
    20881 gttaattgga ctttccaagt tcataaacag tccaatctta tgcttgacaa tttcttttac
    20941 tgctttattt gctggaatca cattatggcg aagagacttg ttgatttctg tttcactaaa
    21001 ggtttcttta gctactttcg tccagttgac taaaagctcc gttccaacat tgaatttgcg
    21061 gatcccgtta ttgatcaatt caggataatc ttcttctttc acacctgttc ctccatggat
    21121 aactaggggg acatcaacta ctgcatcgat ttctttcagc aagggaatgt ttacttctgt
    21181 ttttgatttg aattgtccat gattcgtacc gatagcaata gctaatgcat ccacgcctgt
    21241 agcttctaca aatttcactg cgtcttccgg tcttgtatat accttgtcat tttcagcgac
    21301 atggattcct tcttctgtac cgccgattgt tccaagttct ccttctacgg aaacaccgtg
    21361 cgcatgggca tattctacta ctgcttttgt cttcaagata ttttctttga aaggtagatg
    21421 ggacccgtca tacatgacag aagtatatcc gctatcgatt gcttctttga tatcatcaaa
    21481 gtctcgggcg tggtctaagt gtaagactgc atcaaccatt tcttcttctg caatagctgt
    21541 cattacattg actaacacat gcatgccgat atatttagcg gtatcgacac ttgtttgaat
    21601 aatgattggc gaacccattt cttttgctgc gcctagcatg gcaggaagca tttctaggtt
    21661 atgcgtatta aaagctccaa ctgtaaaatt caactcttct gctgtttttg ttacttcttt
    21721 caatgttgta tacatctttt attgccacct tatcttttta tttgtttcct ttgatcgtta
    21781 cttgttttgc aatttgtcgc atctcatcca ttttgcccat gtagtaattg atcccttgat
    21841 catcaccagc acgagcagca tctgcacgct tctggttata gaggctcatc agttttttat
    21901 acccatctcc gatcgtctgt ttattttcca agctcttttc taaatattgg atggcttgat
    21961 cgttctcatg caattcagaa aatttaagtc caacttgttc ataaagttcc gctaatttat
    22021 gcctatcttg ggtagtggaa atctcttgtt tcaattgtgt aatctctgat tgaatctttt
    22081 cggtcgcttc tggcgatagg gctttttctt cttttgccag aacttttttc ggttcttttt
    22141 cttttttctt aaaaaatcca aacatcattt tccctacttt caattaaaac gctttcaaaa
    22201 ctgggcttaa gatccaggca tataagagtg ctgcggctac tgtatctaca tcggttgctg
    22261 tagcattgat gaatcccatc gaattaaaga tcgttactag caaagcgggc aataatgtaa
    22321 taaagaaacc atgagcaata ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc
    22381 caaatatccc tgctgtccct ccggcaaaga aattggttag cataccaggt aaaatcatcg
    22441 ctaagccgaa catgggcaaa gtaaacatgg caataatcgt accaatcgtt gtcgtaatga
    22501 aacctaaaat gacggcattt gggctataag gaaagaaaac tggacaatct aaggcaggga
    22561 tcgcatcagg aactaatttc atagctatcc ctcgaaatgc cggcacgatt tcacctaaca
    22621 gtagacgtac accagctagc aatatataca cacccactac gaattggatg gcttgtaaaa
    22681 aggcaaacat gatatagttt tgatctcctg ataattcaga agcgaatggt tcgcctgcaa
    22741 ataaaacagt gatgatatat agcgggacca tcacgaccat gacagacaaa taagtatctt
    22801 gcagaaattc gaaatgtgtc ggcaacttga tatcttcgat cgacttttta ttttcgcctt
    22861 tctcaccaaa aagcttagca acaccggctt caaataagta cccgatcgta caaaaatgac
    22921 ctaaagcaat atcatttgat ccagtgattt tacgaatgat cggttgcgct actgccggca
    22981 tagcaatggc gaaacaagct ccgataaaac caccgaccaa gatcaatacg atccctcgca
    23041 gaccggcaaa atagccaaag actgtcgtca tcgttgccat ccataaaatt gcttgaccag
    23101 ttaaaaaaat gtacttccat tttgtgaaac gagccaaaat aatattgaat acaaaaatgg
    23161 ctaagaacgt cagtgcaata tctctaccta aacccaaatc attcattgct tgatcattga
    23221 tagattcaat agaagggatg atcccttgca tatgaaatcc ttcagtaaag attttcccga
    23281 aataagttaa acttccaacg atgatgctcg aaccggcact caatacttga aatcctagga
    23341 gtgtttttaa cgtgccagag atgacttgtc ctgctgattt cttctgcagt agtagaccca
    23401 acatagcaat caatgcaatg gtgatcgatg cctgagtcaa aatattttcg atgataaaat
    23461 tgataacgct catgttttta ccctcttttc tttttgtatt tagataactc ccttatcttg
    23521 cagaactggg gtgatttttt cttcaatttc attttttgaa acaatatttt tcaaatagat
    23581 catcgccgtt ttaggatcaa ctgcgaactt ttcgaactgc gaacgaaaat tttctgctgt
    23641 gatgatcaca tctggtttca tggaaactgc ggaagaaata tcacaatggt ccagtttggc
    23701 ttctactcct aatttgttca aaacatcctc agcagacatc tgtgctgcaa aactacttcc
    23761 taaacctgcg ccgcatacga ataaaatgtt caatttgttc atctttgttc tcccccgtat
    23821 catttattat ttctcaacta ctgaaaaaag cagttgtttg aaatcttcga tatttgtgca
    23881 ttcaatcaat cgatccaact tctcttcgtt gttgatcaat tcaattagtt ctttcatgat
    23941 gttcagatgg gagtaggagt ctactgctgc caaacaaaaa ataatcttta ctggatccat
    24001 ttcaggatta ccgaaatcga ttggttgctc aatcgtagct acgctgactc ctaatttatt
    24061 gaccccatct tctggtcggg catgggctag tgccatgtgt ttgccaatca cgatatacgg
    24121 tccgtaatca ttcactgcac gaatcatcgc ttcgacatag cgctcttcga caatgttttc
    24181 tttaaccaat ggtctagcag ctagttcaat cgattcctcc caagtagtgg ctttttcttt
    24241 gatcatgata ttgctgtccg tcaaaatatc ttttaacata ggctggatct cccttttgtt
    24301 gatttctaaa ttgtttgttt cgaataattt ttctaacttg ccatagatga ttcgatctac
    24361 tcggccacca ctttctttca tgacatctat tatttgagtg aataactccg tcgattcatg
    24421 actgttaggc tctaaccgct gtagttcttc atttcttttc aaaaagtctt ctataaccaa
    24481 gatattcgtc tcagttaaaa tcggatcgac aactaaaagc ggcttatttc gataattcaa
    24541 ttgaaaggtg ctgaaaacca gattgacatc caatttatct actaattcga tttcttttga
    24601 acttaaaact gcagtaatct cgatctgagg aaacttttct ttcagattct ctgctaacag
    24661 atttccagta gccatcccat ggttacagac aacgactgat ttataaacat aattcaagtc
    24721 tcgcttgatt acactggcga tcgtcgaaaa gtggatgaca agaaaagcta tctcgtcctc
    24781 cagtatcttg ccaccaatta cttcttcgat cgtaggaaca aaatgagtga ctgcgcggta
    24841 gattgggaaa taatttgttc ggatgttttc tttcaatgga ttcatgactt ggatatgatt
    24901 cttgattctt acaatcagtg cagccatgtg cttatagaga ttttcgaata aggcttcttc
    24961 ctttagatgg aaaggtacgt gtgtctcgtt ctctacaaat tggatcaatt ggatcgttag
    25021 taattgagct tgtacccact cgattgaatt ggagatatct ctagcattga acgtctcaat
    25081 agtgaaacga agataatttc tttctacttg cgggacattg tccaattgaa attcctcgat
    25141 catgtggtca ataaatgcag aaaagctact gtcttttatt tctgtatttt tccaattaat
    25201 ggcagagata aattcatgtt tcctgattcg ctggatccag atcaatgtaa aaagcaggat
    25261 ttgtttttta taaatatcat cttcttgttt caacaactta ttggaataga tctcctgtat
    25321 ttttttgatt tcctccaaag gaaaataatg gagaaagatt ttttgagccg gtgttaattg
    25381 tgcatcttga ttcataaaga agtcggtcat tcccagattc tgattgataa aatcatagat
    25441 catcgtacgg atagatcgtt ctgaaccttt ataaaccagt ccttgctttc cataactgac
    25501 gatctctatc ccataactga ccagtgttgc tcgtaaccgt ctcatatctt catctaatgt
    25561 acttttagag atttgaaatt cttcttcttt tttgtttaga tggatcggga ttcggctgta
    25621 agcaacggct aaaactagat caaaaatacg ttcgcgccga ttcaaaaagc cgtctttcac
    25681 atcttttatt tcagtttcta atcgagcttg cttttccttt tccagcttca acaaaaaacc
    25741 tttgttgcga attgtacgga tctccggaag ctttttactg ctcaaccaga tattcgcttc
    25801 aagaacttcc tgacgaatcg tccgctgact gacattgaac tcacttgcta gatgttttgc
    25861 ttttactgga tattgggctg tcatgattct gtgaagtatt tcaattgttc ttgttttcat
    25921 agaaatgatc tccttctgat ttcaagtata tggttccgct tacataataa aaagtagata
    25981 tttctgccac ttgaagtgga aagaaagtaa gcaaagcaaa caaaaaataa gccatatgct
    26041 ttttcatctc ctcaacgaaa aagacatacg cctattatta caaaaatttt gcccatctat
    26101 acaaaaaaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacaa ctaaactaaa
    26161 atagtgcttc ctattttttg tttggttgtt cttattatcg cagatgcaag cttttaaaca
    26221 ccatttagtg gagaagctgt gacagagatg ttcagccttg agcgataaga agcgattttg
    26281 aaaaatcgct tcccatattt tacaaaatcg tgccttattg ccgataggct atctctggaa
    26341 caccgtttat tcagaaaaag gagctgcgac aaggcttttg tcacaactcc gttcataaat
    26401 atttcctgtc aatccagcat aatcggttga ataaaatcgg cttgccatct tttttctttc
    26461 tcagaaaaat caaaatacca tatctgagaa aaatactgtc gttgtgtctt acttcctgag
    26521 atcaatcgcc catcttttcg atcgactgaa taatcacagc atctgaaatc aattcggatg
    26581 gcaaaactat tttcgtggat ctgtcgataa tttcctactt cctgaatagt tattttttta
    26641 gtatgatttc ttacaccgtc tgctttgttt ttttgcagaa ctttctgatg ttcggcaaac
    26701 agcttgtctg tataacagct tctggctttt tcaggagttt cttcttccca agcggattgg
    26761 atcttcatga atatcttttg gatttccaaa aactgtgctt tcttttcttt agccgttcct
    26821 gacatgcggg acaatatctc cgatttcagc cttttctttt gtcttttatt cttgacgata
    26881 gaaaaagcag aaacccctcc tactgacaaa acagctaatg caagaaaccc cgtttgctca
    26941 taactagaag gacctgtact tggaccataa tacccataag aatctctgcc gtaagaacga
    27001 ctgccaaaat aactgccccc atatgaacga ccgccaaatg aacctctgcc cgaacttcga
    27061 ccgctagatg tacctccacc atgaccaccc gcgacagcga atccttgggt cggcagagca
    27121 aacaaacata ggcccactaa caaaatgatt gctaaataat tgattttttc attgtcttcc
    27181 tcgttatctt ttattacaaa aaactatttt tatcatagcc ttgctaaaag gaagagtcaa
    27241 taaccagatg cataaactgt ttttttagca attttgtctt atttagtcat tgagtaacat
    27301 cggacagatg tatgtgtcgg cagtccataa ttcctctatc ttcagcatga acaagcttgc
    27361 agacaagaga tctgcgcttc ctccaggaga gatttggttt tcctgacaaa agtgcaacag
    27421 tgctgtaaat ggacgatttg gtaaagcact ttgactagca gcatgagcaa gtttttgcat
    27481 ttccactaac gttttctgat cactgcgatg caatacgcat gtgtcatcta gcgaactcat
    27541 caaaactaac aacatatgca tttgtttctg tacatatgaa tcattatcta gctccttagc
    27601 tgcttggatt gcaagcctga tatgcggata ccctaatatc gcttcttcat atgcaccatt
    27661 tacgccatat ttttgcttca ccctatgacc atgagtggtt tttttatgac gatattttgt
    27721 atcaggaaaa gcagcaagct tgctgacatc atcaagtatt ttcgataaag aaaaaggctg
    27781 ttccatactg attttttgtg caatgacact gatcaatagc cccattgccc aaatcgctcc
    27841 tttatgggta ttgatgccat ttgtagcaat cagcattgcc ttttccgctt ttcgcccaat
    27901 ctcagcaatt ctttctctta actcctgatc aatagcataa tgccatgcta tttgagccat
    27961 ttcttcaaaa aaaggagtta gcgtcttagc agacttatcc aaaatgcgcc gaccttcttt
    28021 tacggctttt gcgatcgtat ggaaattatc atctcctaaa accatatcag ctgcctgttt
    28081 gcttacttca ctacctttga ttcccatagc gattcctata tcggctcttt tcaaagcagg
    28141 tgcatcattt acgccgtctc cggtcattcc gacgatttct ccatttttct gcaaagcttt
    28201 cacgattcgc agtttgtgtt ctggtgtcgt tcgagcaaag acgtcgactt ttggtacttg
    28261 tcgaatcaac tcttcttcag acataagatc caaatcgatc ccttctaaaa ctttagcagt
    28321 atgttttaag ccaatttgtt ccgcgatcgc ttttgctgta tctttgtgat cccccgtaat
    28381 cattttcact gagattcctg cttgttggca ttcctttaca gctttgactg cactttcttt
    28441 tggcggatca atcaacccag caatccctgc aaaagttaat tgggttaaag actcatgcgt
    28501 gagttcctgt ttcgaatcta cttttttgta agcaaatcct aaaacacgct gacctttttt
    28561 cgcaaacgct gtggctgttt gaccccatgc ttcttattct gaagaagaaa gagttgattt
    28621 agccaataat acttctggcg cacctttgac gtaaatcacc cctccttttt cctccgcatg
    28681 catcgttgcc atatatttgt attcagagct gaacggaatt tttgcttcta tttttttcaa
    28741 aggcagatcg gctgtttcgg tgtggcgaat cagtgcaagt tctgtcggat tgccacttaa
    28801 attttctgtt ttctgttctt ctttcgtctt taattcctga caatttttca taataaacag
    28861 tacgcgagtt tcttcctttg ctgctacatc cacaactgtc atttcatttt ttgtcaatgt
    28921 ccctgtctta tctgaacaga tcactgtcat agaacctaat gtttctacag aaggcagtcc
    28981 tttgatgatc gccttttctt ttgccatttc atgtactccg acagatagga tcatggtcaa
    29041 cacagctggc aatccttcgg gtaccattga caccaccaaa gcgatcatag ctgaaagaag
    29101 gatatgccaa tccatgccat agcggaatgt tgtaaaaaca actaagaaca agatcaaaca
    29161 aagaatgcct tgaaaaattt gtttgttcaa tcggttgatc ttcttgatca gaggcgtcgt
    29221 ttgttgttcg accgattgca gtgcttgatt gattttaccg atttctgtcg tgtttcctgt
    29281 ttctacaacg acaccgactg cagaaccaga ttgtacaagc gttcctgaaa aagccatatt
    29341 tttttggtca ccgattgatt cttctgctgc caaaacggaa tgattttttt cagatgcttc
    29401 tgattctccc gtcaaaatcg attcttctac tagtagatca tgtacttcga tcaaccgaac
    29461 atctgctgga acgacgtctc cggcacgtaa gacaatgaca tctcctttta ctagttcttt
    29521 cgcagggatt ttttcttgca tcccgttcga tagtactatc gcttcttgtc ccatcagctg
    29581 cttcaaacca ttcaacgatt cttctgcttt tctttcttgc caataactaa cgagtccgtt
    29641 gacgatcact acgaataaaa taatacttcc ttcgatgtat tcgctcgaag caaatttcaa
    29701 aaaagaggca atgatcaaca cgatcatcag cagatcagta aagtgttttg ctattttttt
    29761 ccatcgtttt gtctgctgtc ttgcttctat ctcattatat ccttgatcag tcaggcgttt
    29821 ctgccgttct tcttgcgaca agccttctgt tgatgtttga tagtttttca atacatcttc
    29881 taccgtcaat ctgtaccagt tcatcctatc ccctcctgtt taattgatac tgttattata
    29941 cgcagggaag ttttttcagg cgtatcgatt atttcagggg ggtctgaaat aattcgtttt
    30001 gcagaatgta tctgggccta actgatattt ctattgctta ttcttttata tacaaaattt
    30061 aagaatacaa accagataac caataaaaaa ggaataaagc gtgtcgtgct ttcgaataac
    30121 aagctgaaag caattgaagt aaaaaacacc aacaaagcaa gatctttata tctttgtgta
    30181 gtgttccgtt tcttttcttt aataaattga acatgtgcac acaagatcga agaccacaca
    30241 aatagaaaac aactcgttgt catgcctgcc aaaagctgaa aaaacaatcg actatcagct
    30301 acaaaaaaat gtagacaaag accgacaaaa aataacatcc ctgtaattaa aataccgtgt
    30361 aaaggtacgc ctttcttaga aagcgcacaa aaccaatggg gcaaactttt ctcttttgct
    30421 gtttgatata aaatcctgct agtactgtag actgcactat ttgctgagga caaagaagca
    30481 accaaaatca ttagattgac aattgctgct ccgctttggc aatggatcac ttgcagcata
    30541 gtcactaaag gactttcttt taaatctaaa caatgccatg gaacaacaaa taaaatcgac
    30601 aaaatcgttc ctatatagaa aaagaggata cgccaaggca gacagttgat tgcatttttc
    30661 aaattctttt gtgggtcact cgtctcacca actgttaatc cgatcatttc aattccagca
    30721 aaactgaata aaaccatagg aaatgaagca atcattccag caaagccaaa cgggactaat
    30781 tcattgagct gtccaaaatt tgctttgatt ccaatagact gggtatgaaa atctgagaca
    30841 ctcaatccta tacccaataa gataaaaagg aaaatcccca agatcttgat gattgagaaa
    30901 aaagattcca attcaccaaa ccacttgact gcaacaaggt tgactgccat taagatcatt
    30961 agtgcgctac ctccgcttat caaagctggt acatgtacaa accagaaatg gatatacaat
    31021 cctattgctg ttaattccgc aagtccagct ccaagccagc aaagccaata agtccaattg
    31081 acaaaaaatg cccacttttt gcctaaataa tagtcaacga aatcactgat agagttaaat
    31141 gtactatttg ctaaaaataa atcacccatg acccgcatca aaccataaat agccaaaccg
    31201 catatcaaat aaacgagaat aacagctggc cctgctgatg caattgcctt tgctgaacct
    31261 aaaaataatc ctgttccaat tgcgccacct aatgcgatta actggatatt tcgattagtt
    31321 agagtccgtt tcattgccgg tttttccatc aagttcacct ttttcttttt caagcagcat
    31381 ggcgacttcc tgttcccaag aaggtttctt tattttttca atacagagtg gtactacaaa
    31441 caaaatgaca ctgatggctg tgactgcaat tagattgatc catgtcccag ctttcatcgt
    31501 cagtccgaca gaaaatccga ttgccgcaaa taaaacaata acgacagtcc aaactccaag
    31561 attgctcttt ttctcttttt ttccaattct aaatggtcga ttcaactttg catgtgtata
    31621 acgcaatctc aaaactgcga tccccatgat gacatagacg atacagtaga taacagtagt
    31681 tgaatttacg agcatcaaaa atgcttgatt gacgccagga atcagcaaat aaaaaagtgc
    31741 aaagattgag ataatgacag actgagtcaa aataacggat ttggatacgt caaattgatt
    31801 cgtcttccaa aaattgaatt ttggtggata cgcacctttt ctagcacttt ctgtcactgt
    31861 ctttgctggt cctgaggccc aagcagataa ttggactgct acaccaatga agacaagtaa
    31921 actaaacaga ttcacaataa gatgtggcaa accaagaatt tggcaataaa tcgaaataga
    31981 ttgcgcgata ttattcaatt ccatttgtcc ttttggtacg acatttgcta caactaaagc
    32041 attcaaaata ttgacgaaaa atataaaaat taacgcggca aatactccgc gaatatacgt
    32101 tttaactgga tttttcaacc gagtgatata cacagaggac atttcgatac cagtaaaaat
    32161 aaacatgatc ggcgcaaaat aaacaaatga ggatgcagtc gtagtatctg gtattaattt
    32221 gtcccatgaa aaactgccta atgttccggt tggtacgatc ccaactttga tccacgcagc
    32281 aaaccctaat aagagcatca tcgtcaaagg cacgtacaat cctaaccaaa caccgacttt
    32341 tccgccgatt tttgccatat caaatttcaa attcaaaaaa gtgatgatcc aataaacgac
    32401 taaaatgaca actaatgtaa atttgctgtt caaaccaagc ggcacattat cgataatatt
    32461 accaaacaac ggagcaagtg ctgaagcgac catcaccatc ccaggaaaca tttggaccca
    32521 taacagccat gagacaacga acccccattt ttttcctaga gcattcgata cccaaagttc
    32581 tggaccacct ttttggggaa acattccggc aaattcacca gagatcaatg agattggaaa
    32641 agcgtacaat aatactgcaa ccagcatgta aaaaatcatc gtccagctcg ttgctgctac
    32701 atctggaata tttcgtacac ttgctacaag ggcgcaggtc attccgataa aggtcatggt
    32761 cgtgatttgc tttgatgaac cagtatcttt catctttatt ccttctttca atagcgacat
    32821 atgtttttgt tttcttttat ttttgttcaa gaacagaaat gacaaggttt catcgattca
    32881 gtacaaaacc cagtatcacc gaaacatcca ttgaacgatc ttagtgaact gggtttgata
    32941 catgaaacga acgatccaac ttatagaacg ttttataaaa aagaatatct tggcgaggaa
    33001 tatatgaaaa acagggggaa atacaattgt tttgatgaag caaataattg tctgttctcc
    33061 atatgtctct tgttattctt tcacacaaaa atcagttcta ttggatgatt tcgtttagtt
    33121 tcttttccat tgctttggcg atttcttttg tataaaaatt gaaaattcga tcatcattca
    33181 aataaggagt catgatagcc gcacgtagca gagtgatttt ctgttctttt ttccattctt
    33241 ctttcgttag acccattctt tcaacaaaac gaatcggaga atcaccgtaa tcttcttgtg
    33301 taaatgtagt atgagaagta ataaaccgtt caccataaac atcgccatcc atataagaag
    33361 accgatcgaa cattttttca tttaattcat tgatagcgtt caaatctgta cagccttgtt
    33421 ctttgaatac ccaattgacc atattaaaat caggatgatt caatggatag acttcgattg
    33481 tttttccttt aacagtaaaa gtcagatgat cgagaaattc tctgaatctt tgagctgcct
    33541 cgattgaagc cccaatcaat tgaccatatc cggtcacatt caatggcaat acacgatgtg
    33601 ctgtccagac tgcggcggcg gttgctccag cttttgatcc ttccaaaata taagctccta
    33661 acatatctgg agcctttact gatttttcaa acacatatgg cgcgaaataa gaaatgatat
    33721 ttctcatatt tttatgtttg attacgattc ctccagcagc ataagggaca tagcccatct
    33781 tatgaggatc tatcgtgaca gaatccgctt ctgaaatagc tctgaaccct tcatatacct
    33841 ctttgtcaat cgttacgcag tggtggaaaa catgatgttc ttcaaagaat tcagctaagg
    33901 aagcatacgg cacaaattct cctgcttcgt ttagaaacag tgaacgcgca tagccgccat
    33961 atgcggcatc aacatgtaaa taaaaataga tcccttcatc ttttaacctt tctcttaatt
    34021 ggacgatttt atctacactg tctacttgac cttcttctgt cgttcctacg acagcaacca
    34081 cacctagaat tgggattttt tgttcagcta attcacgaat tgttttttcc aaagcattaa
    34141 tatccattcg ataatcctct tgaactggga tagcaaccat ctgatctaag cctactccgc
    34201 aaatatctaa cgccttcatc caagaatagt gttttgtttg aggcaccagc catttcccta
    34261 atctttggat attttttccg ctacgggaag aagctgcttt gacctcatcc atttcttcgt
    34321 ccgtaaagct ttcggtcatt tccaaaattt cttcaacaga taaattcaaa agttcccact
    34381 cagacatttt tttcacttgt tcaggcagca cttccttgac agctaaagga atcgatttga
    34441 tgcagcgggc gtaccataat ccttcaagat tagcaataga accatctgct gcaatgtgtc
    34501 cccatccgtc tgtcatattg aataatgaag caaaatcctg tcctacttct gcttccattt
    34561 gcgaagttgc catagaagat tccaatgcaa cattatttgg gttccacagc atcgcatagt
    34621 tgtaagctaa taaagcaggc atcaatgttt cagcattcat ttgtccccag taacggcctg
    34681 ctgaatgcca tgggatcgat cctgcgcgta atcgctgact gacttcatcc aagacctcta
    34741 tcatatggtc gcgggtggct agataatctg gagtgagctt atctccttcg ctaattgcag
    34801 gcatatctga aggaatataa ttttctctcc atccaagatg ttcatctacc atcttgttca
    34861 acaacatttt ataaactggt ccattttcag ctttgtcgcc tataaagacg gccttgatat
    34921 ccatatcttt catttttctt ccctccatat tttgttggtc aatgtctcgc agatacatgt
    34981 gtttatctgt aacacctctt cactatagcg ccaaaaatga gaaatttttt tttataacat
    35041 caaatccacg tagattttta c
//
LOCUS       DACGLH010000023        34789 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.025, whole genome shotgun sequence.
ACCESSION   DACGLH010000023 DACGLH010000000
VERSION     DACGLH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.025"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(233..1081)
                     /locus_tag="IXT21_001215"
     CDS             complement(233..1081)
                     /locus_tag="IXT21_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4868632.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IXT21_001216"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IXT21_001216"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ4868633.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IXT21_001217"
     CDS             complement(3582..3761)
                     /locus_tag="IXT21_001217"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ4868634.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IXT21_001218"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IXT21_001218"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ4868635.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IXT21_001219"
     CDS             5909..8188
                     /locus_tag="IXT21_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4868636.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IXT21_001220"
     CDS             complement(8281..8928)
                     /locus_tag="IXT21_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868637.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IXT21_001221"
     CDS             complement(9107..9826)
                     /locus_tag="IXT21_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4868638.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IXT21_001222"
     CDS             complement(9957..11108)
                     /locus_tag="IXT21_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ4868639.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IXT21_001223"
     CDS             complement(11325..12065)
                     /locus_tag="IXT21_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4868640.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IXT21_001224"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IXT21_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ4868641.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IXT21_001225"
     CDS             complement(12449..13042)
                     /locus_tag="IXT21_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4868642.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IXT21_001226"
     CDS             complement(13026..13673)
                     /locus_tag="IXT21_001226"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ4868643.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IXT21_001227"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IXT21_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ4868644.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IXT21_001228"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IXT21_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ4868645.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IXT21_001229"
     CDS             complement(15119..15646)
                     /locus_tag="IXT21_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ4868646.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IXT21_001230"
     CDS             complement(15826..16611)
                     /locus_tag="IXT21_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ4868647.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IXT21_001231"
     CDS             complement(16604..17473)
                     /locus_tag="IXT21_001231"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ4868648.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IXT21_001232"
     CDS             complement(17474..18853)
                     /locus_tag="IXT21_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ4868649.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IXT21_001233"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IXT21_001233"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ4868650.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IXT21_001234"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IXT21_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ4868651.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IXT21_001235"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IXT21_001235"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ4868652.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IXT21_001236"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IXT21_001236"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ4868653.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IXT21_001237"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IXT21_001237"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ4868654.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IXT21_001238"
     CDS             complement(22811..23035)
                     /locus_tag="IXT21_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ4868655.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IXT21_001239"
     CDS             complement(23088..24050)
                     /locus_tag="IXT21_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ4868656.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IXT21_001240"
     CDS             complement(24060..24500)
                     /locus_tag="IXT21_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4868657.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IXT21_001241"
     CDS             24806..25771
                     /locus_tag="IXT21_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ4868658.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IXT21_001242"
     CDS             complement(25828..26058)
                     /locus_tag="IXT21_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ4868659.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IXT21_001243"
     CDS             complement(26144..27031)
                     /locus_tag="IXT21_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ4868660.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKKDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IXT21_001244"
     CDS             complement(27120..28343)
                     /locus_tag="IXT21_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4868661.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IXT21_001245"
     CDS             complement(28360..29439)
                     /locus_tag="IXT21_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4868662.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IXT21_001246"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IXT21_001246"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ4868663.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IXT21_001247"
     CDS             complement(31297..31563)
                     /locus_tag="IXT21_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ4868664.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IXT21_001248"
     CDS             32042..34276
                     /locus_tag="IXT21_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ4868665.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="IXT21_001249"
     CDS             34424..34744
                     /locus_tag="IXT21_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ4868666.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9843 a         7508 c         6064 g        11374 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatctttt tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcgtatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atagtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaa
//
LOCUS       DACGLH010000024        34578 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.026, whole genome shotgun sequence.
ACCESSION   DACGLH010000024 DACGLH010000000
VERSION     DACGLH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.026"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..1537)
                     /locus_tag="IXT21_001250"
     CDS             complement(<1..1537)
                     /locus_tag="IXT21_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868667.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKE"
     gene            1659..2243
                     /locus_tag="IXT21_001251"
     CDS             1659..2243
                     /locus_tag="IXT21_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868668.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2254..2499
                     /locus_tag="IXT21_001252"
     CDS             2254..2499
                     /locus_tag="IXT21_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868669.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2505..3227
                     /locus_tag="IXT21_001253"
     CDS             2505..3227
                     /locus_tag="IXT21_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868670.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3358..4101
                     /locus_tag="IXT21_001254"
     CDS             3358..4101
                     /locus_tag="IXT21_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868671.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4107..4472
                     /locus_tag="IXT21_001255"
     CDS             4107..4472
                     /locus_tag="IXT21_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868672.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4488..5018
                     /locus_tag="IXT21_001256"
     CDS             4488..5018
                     /locus_tag="IXT21_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868673.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5180..5488
                     /locus_tag="IXT21_001257"
     CDS             5180..5488
                     /locus_tag="IXT21_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868674.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            5484..5903
                     /locus_tag="IXT21_001258"
                     /pseudo
     CDS             5484..5903
                     /locus_tag="IXT21_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6040..6840
                     /locus_tag="IXT21_001259"
     CDS             6040..6840
                     /locus_tag="IXT21_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868675.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6877..7059
                     /locus_tag="IXT21_001260"
     CDS             6877..7059
                     /locus_tag="IXT21_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868676.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7170..7487
                     /locus_tag="IXT21_001261"
     CDS             7170..7487
                     /locus_tag="IXT21_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868677.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7490..7722
                     /locus_tag="IXT21_001262"
                     /pseudo
     CDS             7490..7722
                     /locus_tag="IXT21_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7837..8316)
                     /locus_tag="IXT21_001263"
     CDS             complement(7837..8316)
                     /locus_tag="IXT21_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868678.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8436..9773
                     /locus_tag="IXT21_001264"
     CDS             8436..9773
                     /locus_tag="IXT21_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ4868679.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            9796..10323
                     /locus_tag="IXT21_001265"
     CDS             9796..10323
                     /locus_tag="IXT21_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868680.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10310..10873
                     /locus_tag="IXT21_001266"
     CDS             10310..10873
                     /locus_tag="IXT21_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868681.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11295..12032
                     /locus_tag="IXT21_001267"
     CDS             11295..12032
                     /locus_tag="IXT21_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868682.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(12049..12465)
                     /locus_tag="IXT21_001268"
     CDS             complement(12049..12465)
                     /locus_tag="IXT21_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868683.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            12858..14105
                     /locus_tag="IXT21_001269"
     CDS             12858..14105
                     /locus_tag="IXT21_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868684.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            14373..16763
                     /locus_tag="IXT21_001270"
     CDS             14373..16763
                     /locus_tag="IXT21_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ4868685.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            16812..18647
                     /locus_tag="IXT21_001271"
     CDS             16812..18647
                     /locus_tag="IXT21_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4868686.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            18722..19579
                     /locus_tag="IXT21_001272"
     CDS             18722..19579
                     /locus_tag="IXT21_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4868687.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            19569..20678
                     /locus_tag="IXT21_001273"
     CDS             19569..20678
                     /locus_tag="IXT21_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4868688.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            21017..21817
                     /locus_tag="IXT21_001274"
     CDS             21017..21817
                     /locus_tag="IXT21_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868689.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            21905..22711
                     /locus_tag="IXT21_001275"
     CDS             21905..22711
                     /locus_tag="IXT21_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868690.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            22943..23812
                     /locus_tag="IXT21_001276"
                     /pseudo
     CDS             22943..23812
                     /locus_tag="IXT21_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            23832..24179
                     /locus_tag="IXT21_001277"
     CDS             23832..24179
                     /locus_tag="IXT21_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868691.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            24192..25454
                     /locus_tag="IXT21_001278"
     CDS             24192..25454
                     /locus_tag="IXT21_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868692.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            25534..26910
                     /locus_tag="IXT21_001279"
     CDS             25534..26910
                     /locus_tag="IXT21_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ4868693.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            26907..27536
                     /locus_tag="IXT21_001280"
     CDS             26907..27536
                     /locus_tag="IXT21_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868694.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            27555..28034
                     /locus_tag="IXT21_001281"
     CDS             27555..28034
                     /locus_tag="IXT21_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868695.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            28536..28589
                     /locus_tag="IXT21_001282"
                     /pseudo
     CDS             28536..28589
                     /locus_tag="IXT21_001282"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28593..29192
                     /locus_tag="IXT21_001283"
     CDS             28593..29192
                     /locus_tag="IXT21_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ4868696.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            29208..29684
                     /locus_tag="IXT21_001284"
     CDS             29208..29684
                     /locus_tag="IXT21_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868697.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            29701..30603
                     /locus_tag="IXT21_001285"
     CDS             29701..30603
                     /locus_tag="IXT21_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868698.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            30676..30870
                     /locus_tag="IXT21_001286"
     CDS             30676..30870
                     /locus_tag="IXT21_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868699.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            30975..31403
                     /locus_tag="IXT21_001287"
     CDS             30975..31403
                     /locus_tag="IXT21_001287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868700.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            31638..32225
                     /locus_tag="IXT21_001288"
     CDS             31638..32225
                     /locus_tag="IXT21_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868701.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            32225..33124
                     /locus_tag="IXT21_001289"
     CDS             32225..33124
                     /locus_tag="IXT21_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ4868702.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            33124..34038
                     /locus_tag="IXT21_001290"
     CDS             33124..34038
                     /locus_tag="IXT21_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868703.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            34090..34305
                     /locus_tag="IXT21_001291"
     CDS             34090..34305
                     /locus_tag="IXT21_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868704.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        12980 a         4735 c         6816 g        10047 t
ORIGIN      
        1 tttcttttct taaatagtct tcagacttat taataatatt atctattatg tctagataca
       61 tatcactttt cgattcagct aattcataca gtttttttaa ttctttatcc tttaattgaa
      121 aaccctgatc catagtcact gcatttggat atagaagccc actataatgt ttacgtaaat
      181 tcacttggaa tttctgatta taaaagtcta acagattaga aataaaaggc ggaaaattac
      241 tgtttttgta aaattgatta tttccattta ggttgtatag taatttgtta aattgctcct
      301 gatttaattt attgtaaatc catggtaata cttccatatc cttatctgct aacctgaatg
      361 aactagcatc ggtaagatgt tcgatcataa aatctatgaa catctctttt tcaccaaaat
      421 ataaatattg attatatatt gtttctaatg ttgaagatgg tagcgaagaa actctcattt
      481 tttgctggag tataggatca actagactat ttaagtgatt ttgtaattct tcattgttgt
      541 ttcctagaac tgcaagggaa cgtaaacggt agtaatattc tatttctgtt agtacttgtt
      601 gtttcggttt aaaatcattt attgatattt gtaaattttg aattgtctcg tctgaaaaat
      661 catgttgtaa ttctggatgg gtagctataa aagtaagtaa aaagaaccct tttgtatacg
      721 tgatctgttg attttcacca agtgatttat taatttcctc tcttgattca gctgtgaacc
      781 tatactttgc aatttctttt tttagaatat ttaaatagaa ttctttatta tcaaaaatta
      841 gggcattaag tagtctgaaa gtactttccc tatctctatc tgcttcctca tttccataag
      901 ataattgaaa aacaaactta aaggctgtct tacataggtt ttctttggct ttttcgctta
      961 gtttatttag atagctattt tttagtgcag agtgaaattc ttcaggtgta acagacatag
     1021 catgtatgcg acttgcatat tgtataattt cgatcgttac tttttttcct aatattggat
     1081 tttttaggaa tataatttca taggacttcc gtataacatc ctgacaagtt tcttttgtta
     1141 tatctcttaa ttcccaagta ttaccgttgt aagtgactac aggatgagct gaataatttc
     1201 tttgatcctt aatatatttt aaatcctcat attcatagct atcaattagc tcagttgaac
     1261 tataagcttt ttctagtagt tccatttccc attttggaga gtttggatca ctttttctta
     1321 gtctagctat atcttctaaa attttcttag aagtattatc ctcgtaaata tttgctaaat
     1381 cagataattt atcaattaaa tctaaaataa gcgcactgta attgacagct attgcagaac
     1441 gataatttcc ctgataaaaa ctacttctaa cttcttctaa taatggaata attctaggat
     1501 ttttaacttt ttttatttca tcatctaata taaacatata aatctccttt ttttatttgt
     1561 atgacaagtt taccataaaa aaaaattaaa aagcgagaca tatttatgtt gttgtgttat
     1621 cataataaca taaatatgta gagtgagatg atgaaaaaat ggatttgaaa caatcgtttt
     1681 acatctggtt atatcctgat aatcctatgt caataatggc tgatattttt gtagttagca
     1741 tcttattgta tttattttat ctaattttct ttcacaattt aatcgggtac atgattcttg
     1801 attggaaagg aatgttcgat atagaaagaa aaccatttgc taacaatcaa acctataccg
     1861 tcttcgaaag tggagattgg gaagaaaatg aagttgatgt gatccatgaa attggattag
     1921 ctttatcagg aatacaagat gctgatgttt tggtatcaat gacaacggat actggataca
     1981 acgcttatga ggtattttat cttatgtatt ttatatggcg tgatggtcaa gaaattatca
     2041 atggtcgtgc tgaaaaaaca atcaattcta atactgaaat ttctttttgt atgggtgaag
     2101 gggaaatgat tgtatataaa gatgggtcag caaacattaa aacagccttt ggtgatattg
     2161 aaacaagtat taaggaactt agggaatatg tgcagaaccc atataatttt caatatttag
     2221 caggaagaat aaatttagtt taggagtaaa aatatgaaaa aaattataaa tcaaccaaca
     2281 aatcacttga taattgaagg tgttattttc gtaacaatgt attttactgt aggaaatggc
     2341 atctcaaata caatcattca aaaaaatcca caatacctat tatttagtct ttatgcttgg
     2401 attgtacaaa tggtttgggt ttccatattg atgctgttgt tttattggat agtacgggtt
     2461 attcttttat tgttaaacaa aagaaaaaag gataggtgaa taaaatgaaa tcaactaaat
     2521 tgaaggttgt attgttatcg acagcagcat taataaccct tacagcatgt ggtacaacta
     2581 atagtgctaa taataacaaa gacacaaagt ctagtagtac gatggttact aaaaacagca
     2641 ctacatcggc atcaacaaca atgtcttcaa tgactgaaaa taatgctgaa aagtatcaaa
     2701 aatacgaaga cattaataaa ggtcaaaagc cggatgaagt ttataacaac tttattaata
     2761 cagtaaaaaa tgatgaaaat tatgggaatg atccattgca aaataaaaat ctgactatcg
     2821 ttctttacaa acctgattgt ccagattgtc aaaaagcaga atcaaaaatc atgcaagaaa
     2881 taaaagaagg atttagttat gctccaacat taatgcatga tattaccata aataaaccag
     2941 cttacgagta ttacgcagtt gctgtagata ttactgatgg tgtacctgag tgggtacaaa
     3001 acattcctgg tctggctgat gagaacaatc aatatcatac gccaacagtt gcagtagtaa
     3061 caccaaagaa agttaacggc acactatact gggatgtaat gggaatgaca acaaatacaa
     3121 ctgatgaaat gattgaaagt acaactaaat attatatgga tttgggagca tataaattat
     3181 atcaacaatc agagcctgtt atggacgatc aatccaaagt taaatagatc tttagggcgt
     3241 aagccctatt tttttaaaat taagtgataa caaataaatg atttctgaaa caaacgttct
     3301 tactcaacta ctcgaaacac acaagctaat taactagatt ataaaggaga atgcgtaatg
     3361 aaagaaatca gtgcaaatga agctcatatt ccgcaaaaga atcaggttgg tatatataag
     3421 gtgcttcatg gtgaaggaat ttataaaata cagttactta ccggttacaa taatggtgca
     3481 aatgggaagt ggttaaaggc aacaagtatt cgtgaactta gaagtgaagt agaaaaaatt
     3541 aggcttggtc tcacaagtga agaaatagct aaactgaagg gaaataattc ttttgtaatt
     3601 gaaaaagaaa aggaaaaaca tctactttat cgtgagctga aaattgaaga atactatgaa
     3661 attgatttac ctgaaaaatt aagaattatt gatcaacgtt cagaagaaaa atggaaaact
     3721 gaaatatatc caagcttaaa tgactacaag acatcaggaa agcccatgta taactacaca
     3781 ggctttttaa cctacacaca atacaaatat gcctttcgca aattaaaagg cattaaacaa
     3841 gctgaaattg ccctgtatga gatgaaaaaa gtggatagat tgaaattgat tcaagattta
     3901 gtgcaaaata catggaatca agcgattgtc gagctagaaa aagaaaatgg gaaaatttta
     3961 actaataaat atctgatcgt aaatgaaatt gaagaattaa ttggtttatt ttcatgtgaa
     4021 aaaggtgaag tcttcttgaa gtcatcacca acctattcaa ttttggacag tccgtatcag
     4081 tttatacagg caaggagatg agttagatgt ctagcttgaa agatgactat atccaagcaa
     4141 aacaagagta tgaaaaaata cagatgttga aatctgagaa agtcgaatta tttttaacac
     4201 aaaaattagc agaggatcat attgctaact tagatgctaa aactttagag gaatatgatc
     4261 ggcagtttaa tcagatacat cacaatatca ttttgcttgt tgagcaggca aagcgaaggt
     4321 ttgaaaaagc ggaatatgat ctattagatc aaggaattgt atgcttggat aagggattgc
     4381 gaagatatta tggtgcagag ttaaaaaagc aaaatgtaga tagaaatgca ctaaaacatg
     4441 aaatcttaga attgataatg caacgaattt gaagggatag aaagcaaatg tcacaaaaca
     4501 ttagtgctgt aaaacaatta atagaagaag atttagagac cacagttcag agattagcgg
     4561 aacgaatcca ttttgcaaat tatcaaacca tctctaactg gtcaagcagg aacagaaatc
     4621 cggaagaaat tccaatgaaa tttttttatc gaatcggaaa agaatatggt gttgatccga
     4681 taaaaatttt cgagaagacc atgatttatt actatcatgg aacacctaac gaagtgttag
     4741 aacatctttt ggaagaggca aaagaaaaat catcaatatt agagctatcg caaaatgaca
     4801 aagttattca agtgaaatgc gttgatccaa caggaaagat tattgtcggt tttgatattg
     4861 aaagaaaatc caaaatagat tttattacac aattaaaaaa gataaataat ttagatattc
     4921 taacgcttag accagatcat gaacagttgt ttatgagttt taaaaaacaa ttgaaagaaa
     4981 tacaaaaaag atataacaag gtgtttccta atgcgtgaaa agctcaaaga aatcagagtt
     5041 ttttggataa ttaacaaaga atgtatcatc ttatatactc ttggatttct tagcatgatc
     5101 ttgttttggt ggttatattt aaaacagtaa ttggagaggg tcaagaggat ttttatcaag
     5161 ataaggatgt ggaaactgca tgtggaataa tagattaaaa acaggattgt tattaatagt
     5221 tatttcctgc gctatgatga ttggtatgag gatacaaagg gaacaatcct attttgaagt
     5281 ttctgctaac aacgtaatag aaaaatgcta ttacggtcaa cattattgga gtgaggaagt
     5341 cagagaaaat atagatcgtg aatatgttca aagaattgtt tgggatgcat attctatcaa
     5401 agattatcct aaaagtttaa cgagtagatt attttacagt gaaaaagata atcaaaaact
     5461 ctcagatttg atgatgaaaa aagtatgact attattacga aagagaaaca gagtgctata
     5521 ttcgagggga aaaagcctac aacaaaagtg actattgggg tgacaaggcg aagcgattaa
     5581 ctgcaaattt tagagtgtat gaatcagagt ttataaactt gacctttctt aatagtgttt
     5641 ttattgatta tgtaattact acgaaacgca ttggaagtta ttcaagtacc tacgcatcct
     5701 tagttccaat gttaaactcg atgagccgtt tcttaaaaga aagagaaaag aaagagctac
     5761 agtatatcaa agaaatcatg ccggattttg aaatgcacga atggcaaatt gctttatcag
     5821 attggaaatt ggcgaataag gttcgtagaa taacaccttt tcaagccaag cgctttttga
     5881 gagctatgaa atttgtgcaa taaaaaaagc caccagacaa gttgatggag actaacacaa
     5941 ttaccaacag ttgtaattgt gttagcttac aaattaatta tggtgtttag tcaatcccaa
     6001 attgtgaaat ggcaccaata attggaaagg aaaaatgaaa tgtctgaaga tttacgagag
     6061 ctgaagttac gaaagaagca gtatgatcag tatttttccg gtttgttgaa taaacatatt
     6121 gaagaaggac ttgatcatga ttttggaaaa gatgaatatt gtttatattg cgaacgttac
     6181 aatgaaaaaa tgtctgaata tctacagctg aaaaaagaga tacagaaatt agagaaaaaa
     6241 gtacatgctt ggaagtataa agattccatg tctggttatg ttgaatctca aaataagcca
     6301 agaaaaaaga agcaagcgta tgttctaaag atggtaaatg gggaagagtt aattgtatta
     6361 gcgtacagtg aagaagaggt tcgagaactc gttgaaacac ctgtcaggaa aaaggttgaa
     6421 acaataaatg agatagacga cagccaatac aatagcatct atattgaaac aagtcatggt
     6481 ggatataaga aattagcaac tttccttaga agttctaaag ttgggataat ttctaaaaaa
     6541 gacataagtg ctgttaccga tagtttttgt actttatctg ttcctaaagc ttatttccaa
     6601 tacgatagta aaagatttga acgcttagag aaattctgta gaccgtatac tgtattgaaa
     6661 actaaaaaat ttaataaaac taaaagagtt tctatacagt taccgactgg tcaaatctca
     6721 ttaggtgaaa aagactatct gataaaagta ggaaaagagt taattataaa agtaccggaa
     6781 cacttattta aagaaaattt tgagatacgt gaaaccttgg tatggaaaga agaaaaatag
     6841 tttggaagac atagaatggt ataaggggaa attgaaatgg cagtaaataa aatattgcag
     6901 gtactaatca tagacgaatt tggcggagga gaatggtttg atgtgcagtc catagacttt
     6961 gaagaagaag tcgtagttat agaaaatatt tacggcgaag tatattcata ttcgcttgaa
     7021 ggtgagaata ttgaggattt acggttgatt ggtgagtaga aaaaaacaga aatatcaaaa
     7081 tggagataaa aattaatagc atgtaaagat agtcatgctt gggttaaaaa tgttaaaatt
     7141 caaaaaaatg agtcagaagg gataaaagta tgaaaagaga ggaatacaaa caaagattaa
     7201 acgaactgct tgaagaggac gaaacattaa ctcatggttc gcctgatgaa attctataca
     7261 tgattgataa tatggtgatc tttggtggat atgaacttgg taataggtca gttgatcata
     7321 atattttaga atttgatgat gtatcatggg aagaaattct tgattgggga attttggcgg
     7381 tacctgaaac taaaacctat attagtgaca ctatggttcc tttttttgag gaattggatt
     7441 ataaaagact tcccaaaaat gaaaatcata tactgggagg aaactaacaa tgttaagtga
     7501 cttatttatt ttactgatct atctaggtgt aagtgaaatt attgcgtatt ttttgtttgt
     7561 cattgctaat tatggtattt ccagtgcaat cgttcaaatg tttagattac cagtaagtga
     7621 actaaaaaaa tgatgagatt atcagcatat cttttaatgt ttttgaatgt ggtcacgtta
     7681 atctttgtag taatcggtgt gactttcgat ttaatggaat agtgggaaat ctataatgga
     7741 aaaaagagac acgaatgaac atattttttg aatcaatatt gttgatggtt tttgcgatca
     7801 gcgcttacca gaaatctaaa aaagagtaat gattgtttaa tcatcactct ttttttgtag
     7861 ggaaattgaa atatgtggag cttgtattgg tttagttagg attgcttggc tataaggagc
     7921 tagttcttta tcaaaaccat caaagtaggt tttcagtagc gatttaactg atcgctcatc
     7981 ttgtttgatt tggataattt cagcatccaa atcggttgtg agatcaggag cttttgatgc
     8041 gattacctga ttttttttat cttccaactc tcttaggctg tttaacaagt tgtaaaaaga
     8101 atcattttga ttgttcattc caacgtgagt tgcaatttta acagaagttt cgtccgtatt
     8161 gtcatcaaaa gaactcattg gaatatcaag tagtaacgag gatgtttctt ttactgactt
     8221 tttctttaca gcattttttg tttttccatc ttttttgctc tttactcgag atgtttttgg
     8281 agctagttta ggtaaatcac ttgttttatc agtcatttat tagcttttcc tttctattaa
     8341 tttatgttat tatattatca taataacata aaaaagtaca ctttttgggg gcgtagttat
     8401 tgaaaaaatc taagagaaag attgccacat tgcttatgtt aatggtgcaa tttacaggga
     8461 ttgcaatgag tgtaatcaca ataaatccgc aagtgataca tgctgatggg atagctactt
     8521 atgatgcaca atttaactgt actactgaag gtagtgggtc aggaacagat agcgatggaa
     8581 acgatactgg aagtggcgct gtaacaggtg atgtagccaa aaatattgaa gatatttaca
     8641 aatacatgaa tggagaatat ggcttatcag gtgctagtat tgcaggtatt ttaggtaact
     8701 ggaaacaaga atcaggtatt gatcctcgag cagtagaagg gtattacgga gatggacaga
     8761 gagaagttga cttcatcaaa caatttactg atggtctgat gggtattggc tatggtcaat
     8821 ggacagctgg gcgacataca gcattagttg attggtctaa gaaaaataat ggcggttctt
     8881 ggtggactac agaagcccaa ttggacttta tgttcaaagg tgatggggga ttcgtaggaa
     8941 tattaaaaga ttatgcttta aatgcttctg atgatgtcgt agaaaatgca gtaacttttc
     9001 ataaaaactg ggaaatttca gctgattccg aatcagctgt aatagctgta cgtggtgaaa
     9061 atgcaaaaga aatctggaag tacatgaaaa gcaagggtat ggacggtgca aaagatgaaa
     9121 gtaagatcaa taaaatatcc ggaggttcaa gtaccggaga tggatcgaat aattcatcaa
     9181 gttcagcatc aggaacagta gaagatgcct gtgcaactac ggatgacgct aattctggtt
     9241 ctggatcaat tgggaaagaa ggaaaaatta acggtaaaaa aggtcaagta atagcgaata
     9301 tgacttatga agaagctttg aaaaaatatg ggaaagcgat cacgctacca aaattcaaag
     9361 aaccagattg gtcaacttca ccttttaacg gaatatatca aggtcagtgt acagagttga
     9421 cgtgggctta tatgagccaa ttatacgatg ggaatcaacc gactactgga aatggtggtt
     9481 atgtatggga aagctacaaa tcggcaggag caaaaataac gaacaaacca atcgtaggtt
     9541 atgggtttag tgcttcggat ggatggttat gggctgttgg agatgcaggg catactggtg
     9601 tagttatagc ggtgtttgaa gatggatcat ttctatgtgc taactttaat gttccaccaa
     9661 cccaagctcc aacaagggga gtttcggtaa cgttgattga tggagtggac ggatcagata
     9721 atgtgcattt ctttagcggt gtaggaaaag ctaaatttga aatcgaaaaa taaattggaa
     9781 tgaaggacga tatagatgaa tagtaaagtg aaagatacaa ttatcatttc tttgctttct
     9841 ggtatgttat tagcactgct tgttggaatc ctattcttca ataagcagaa tcaggacata
     9901 gaagctgaaa aacacagtgt gcatattgaa gataagctga cattctttaa agaggttaaa
     9961 ccaaatgagg tggatggatt acggaaagat ttagaaaatg atattcaaga aatgttgaat
    10021 ggatcagttc aaaataagtt tgcagatcaa tcaggtgaag aatttttgag aaaactattt
    10081 attgtaacag gtaaaccacc aattactgat aagagtagtg agaaagaaaa aatcgagtac
    10141 tataagcctt ttacttttaa gctaacaaac tttggaatta agacatctag tagtgggttg
    10201 tatgaagcat tgaccgattt tgaagttcaa tacaatggaa aaaaagtgta tgataccaca
    10261 ttaaccatca cactaaataa tgataagaaa gtgactgggg gtgcttttta tgcaaaatca
    10321 tgaaatcact actgctaaaa aggcgatcgg aatactttcc ataattgtga tcctttgttg
    10381 tgtggtacta tttcaaaaga aaaatgctta tgataataat gtcaaagaat tggaagacct
    10441 taaaagtaaa caaatcgaag tagtcaagaa taaccagaaa gtgaaagcta tgcaggaagc
    10501 tcaatacaaa gaattgggaa atgctgaaat taaggaacaa agtaggaaat tctttaattt
    10561 attttacaac tggaattcat gggaaaaata tacgaagaat atgaaggaaa tacaaaaaga
    10621 atttcctcag atagtaaagt ctagtcctgt tgatatttct ggcacgatgg taggcactgg
    10681 taatagtcca atatctagct atactgctga ttactataca acaaatcaaa aaggtaaaat
    10741 ggttgaattg attacacaga caagaaatac tccaacaaca gcaacaacaa caatttggcg
    10801 tggagtaaca agcataggag aaaatcaatt gttttatttg gaggatttag atagctatgt
    10861 aagagcagag taaaataaaa aaagagctaa cggtaccaac gttagctctt tgtgctgacg
    10921 atgataacaa aacctaccga aaaaggattg tttcatcaca aacacaaaca gaaaagaatt
    10981 gataaaaaac ccgaacctca gaatttttat taatcaattc atttagatta taataaattg
    11041 agctgaacat tacaatacgt atatgttgta aaacgtaaaa aaagtcaaaa gtaaatactt
    11101 tcttaaaaag tcaaataaaa ctattaaaga caatatatat ggtttatttg acataagaaa
    11161 atggtctaga tgataattgt aatatagaat tgtagactta aataaagcat aaaatacctt
    11221 ttatgataag gtatagctct taattttaag cagttttaga tataagattg aaagtaaagt
    11281 gggggagaat aataatggca gatcataaat tagaaaagtt attagaagaa acgtggaatc
    11341 caaaagaatt tagtgaattt tttatggaga attttgaaac tgacttagca gtgattgtta
    11401 aagatgctct tagagagcaa gggtatcctg aaactgcaaa ctatataaac attaatttta
    11461 ctttgtatac tgaaaataaa ggaacttggg atttttgggc tactttagca aataaggagt
    11521 taagtgataa atcagacaca ggaattcgta acttttttga atcaaacaga gatgattata
    11581 tgtatgctaa caatcaaaat aaattgaact ttagagtaga gtttgatgaa acacctgaag
    11641 aatttataga aagacaaccg cctaaagaaa atgtagcaaa ggtattggaa gatcgttgga
    11701 attcagatga aatagtttca acaattagcg aactaggcgg acaatatgaa ccgctagtag
    11761 aagcggttag agaagagcta agactgaata agtttcctga tgttcaaaat attgatgttt
    11821 ctcaaattga aataaacgtc aagatcacga ataaacttga ttatggtagt tgggcagata
    11881 tagcattaga aaaatacatc tactcaacgt taaaagagtt tatcgaaaat agaatggata
    11941 tcatgtatct acagcatcca cagtatttaa actttggtgt tgaaatagct acacctcttg
    12001 aagaatggaa aatggagcaa gggcttgact agcctttgct ccattttttt attttacaaa
    12061 tgaatacagt tcgtcgtttt taccaagaat gattgtttgc ccaactttga gattgtcagg
    12121 tgatccttca gcggtttgta aggagaacgt tccattgaac ttatcggata ccgttatagg
    12181 tagctttaat tctgatcctt cctcatcttt gagaattgct gtttttcgtt catcaaaata
    12241 aacagtcttt attaaataat ttttcgtagt gatctcagca gatttgtctt tttttgtagt
    12301 agtaccagtt tctttagaat tagaggtatt ttctacgact attccatctt tggcatttga
    12361 gcttgtgttt atttgattga atattttcag cgacaagata acgctaagaa tagtcaatat
    12421 agaaacaagt gtaattaata ctgataattt cctttttttg ttcatttttt cttaacatcc
    12481 ttttttgttt tgattagtta gatattcctt atctaacaag cctttttagg ctattaaatg
    12541 aagataaacc ccaaaaaaat attgcaatta tgacagtgca atggtactat tattatacaa
    12601 taaattttgc gaaaggggta ggtctaaatc gcaaacaaaa agaaattgaa cctagacaaa
    12661 tacaacttgt ataccgcaag ggaagcaagt ttgattttgg gtaaaggtag aaattatgtt
    12721 acaaacataa tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt
    12781 gacaaaacct atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat
    12841 aaaaacaaaa agaaggaatg aatatgaaga gaaattcgct tggtatggtt ttattggcat
    12901 cagctgtact tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa
    12961 acacaccgga tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag
    13021 atactatttg gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg
    13081 agtttgccga attgaacaat gttgatttgg caaaaggaga acaacggact atcccgattg
    13141 gacgaacttt caacatcagt aaagatacat taacagttaa ggaagcagat ggcgaagtag
    13201 ttagtcaaac tgttcaaaaa gataaacctg atcctattga aaatacggag caagagaaca
    13261 aaaaatccga gactacaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa
    13321 caagtgcttc aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga
    13381 aagaagcatc acagggaaca agtgaaagta gcactacgga agctgatact ggaacatcaa
    13441 caaataactc acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg
    13501 atcaatcaca aacaggttcg agtgatgaaa ctgacagttc tacttcacca agtaaacccg
    13561 aacaaccaac taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt
    13621 gcgaaggaac atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt
    13681 taatgacttt tgttaaatca atgccactat ctcctgacaa taatccaaat aatggttttt
    13741 ctggtgaatg gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag
    13801 tttcatgggg tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag
    13861 gattaaaaga tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg
    13921 aacctgctca acataaacca atgttttggg gcgatgctat ttggaatcga catgtagtag
    13981 ccgttgatgg atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt
    14041 ggattaatgc aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac
    14101 gttaataaaa gatatcgttt atatactaag tagttatata gtctttattt gtccttgctt
    14161 aactacttag aaaacttgtt gctcatggtg ggtagaacta aaaggggtga tgctatgaat
    14221 actagagcgt tgaaacagct tgctttcaac gaaaaaaatg attaataggt gtggtgattt
    14281 taacttagga atgggcgcga aggtcattac cacaccgaca tatcgtggct cattttcttc
    14341 gcctaactgg tataaagaag gagtaacgag agatgaagca tgaaaaaacg ataattttaa
    14401 aatcaaaaat aatttattgt tttgcgactt tagtaatgat gttatctacg cttataatgc
    14461 ctttttcagc aataagagct gaagcagatg tcacaaaacc gacaagtgca aaatttatag
    14521 aaactggcgt aaaaacagac ggttatatta gggttaatat gccgaatcat ccaaatgaat
    14581 ggatgatttc aagtcaattt aaagattctc atggaaatat tggatattgt atggattcag
    14641 aattgccatc cccaacagga agtggagccg gaagcttgaa gtacaaggga gctggcagtg
    14701 atgagttcta tcgtatgttt aaaggtggtt ttccatcaaa aacagcaaag gaactgggtg
    14761 caggaaatga tacagaagca tggtatgcaa cacaattagt ttcatgggtt ttagccggaa
    14821 actttaaagt ttcacagatt gtatggtcac atccaaacca taccgccgca gaaacagctc
    14881 gtgtaaaaaa agcatttgaa aaaatttatg actatgctaa gaatggaaaa gatacgccca
    14941 acactgagtt ttctatcaca gcttctaaga cagctgacga aggaaaatat cacactttca
    15001 catataaaac agcatcaaat aagactggta atgcgaagct cacttttaca tcagcaaaac
    15061 cagctggtat gaagatttat gatgcagatg gtaaagaaat tactaacaac actgtgaaat
    15121 taaattcaag ttttactatt aaagtacctg tgactacacc aagtggtact ttgtcattta
    15181 aaggtactgc taatgttagc acaaccaatc cttttacttt tgatggacga ggtgtgtatc
    15241 aagatgccgt agttatgatc actacttcag aaacaaagga ctctaaatct ttgagtgcta
    15301 aatggacaag agctgttggt caggtaacag ttaagaaagt tgatcaagat ggaaatgctt
    15361 taaaaggagc ggagtttact ctaactgaca ataacaatgc taaatctgtt aagacaaccg
    15421 gagatgatgg gttagctacg tttgatttgc aacaaggctt aacatacagc ttagaagaaa
    15481 caaaagttcc tgaaggttat caaggttcat ttaaaaagga tggtattact gttaataccg
    15541 atggacaaga atttagttac actgctacga acacaattaa aaaaggtaaa gtaaaagtta
    15601 aaaaggttga ccaagatgga aaaccattag ctaatgctga atttaccttg acggatgatt
    15661 catcccaaac acaagcagaa aaaacagatg aaaacggact tgcaagcttc gatattgtag
    15721 caaacaagac ctacagcctt gaagagaccg gagtaccaac tggttataca ggcgtattca
    15781 agcaagaagg aattaccgtt aaagatgatg gacaaacttt tgaatataca gctacaaaca
    15841 ctttgaaaaa aggtttagta aaagttcata aagtcgatca agatggaaaa cctttggcaa
    15901 atgcagagtt cactttaacc gatgaaaaag gtgcaacaca agtagaaaaa acagatgaaa
    15961 atggacttgc aagcttcaat attgtagcaa acaagacgta tagcctagaa gaaaccggag
    16021 taccagtagg ttacacaggt gcatttaagc aagagggtat caccgttaaa gacgatgggc
    16081 aaacttttga atatacagct acaaataatt tgaaaaaagg tgtagtaaaa gttcataaag
    16141 tcgatcaaga tggaaaacct ttagcagatg cagaattcac cttaacggat gaaaaaggtg
    16201 taaaagaagt taaaaagacg gatgcaactg gtctggctac gttcaacatt attgcgaaca
    16261 aaacttataa cttagaagaa acaaaagtac caacaggtta cacaggcgca ttcaaacaag
    16321 aaggaattac cgtcaaagat gatggacaag tctttgaata tactgctaaa aacactttga
    16381 aaaaaggtgt gatcaaagtt cataaagtgg atgatcaagg gaaagcatta gcaaacgcag
    16441 aattcacttt gacagacgac aaaggtgtaa aagttgtaca aaaaacggac agcaatggct
    16501 tggcaacatt taatattgtt gccaacaaaa cgtacaaaat cgaagaaaca caaaacccta
    16561 atggttatac agggaaatgg tctaaagaag gcatcaccgt tgctgacgat ggacaaacct
    16621 ttgaatatac agtaattaat caccttgtac ctgaaacagt aagaacaggc ggaatcggta
    16681 catcagcctt ccttcttgta tttctaattg caggattgag tatggtgcta ctgtattatc
    16741 gagaacagca caaagcaaaa taaaaaagtg ttaatggaaa agaagtaaat atgaaaaaag
    16801 aaggctgacg tatgaaatca aaaaaaattt tttcaaattt aatgctattt ggtactttgc
    16861 taggagtggg attgggcgca agctcaatct cttctagcct tggactaggg gggcaagtag
    16921 catatgcaca acaaaactat aattatcaag gaactggaac gattcatatc cataaagtaa
    16981 caaatactaa tggggatggg aaccctgtct cagatattga taacaaaggg aaagaaatag
    17041 ctttaccaaa tggtgtaaaa cctcttgaag gcgttgagtt ctcttatgtg aaacttacta
    17101 aagatgaagc taaatccttg ggatggacgg atagtgatac tacaaaaggt caacatattg
    17161 atgatgctaa agcacgatta ttactaaaca aaaaatacag tgaagtaagt agaatgaaga
    17221 ctgattcaca agggaatata gacataccta tacaagtaaa tacagacgat ttaacagata
    17281 acttgtattt gattgttgaa acaggaacag tcaaaggtgt agttgaagaa gcgtatccta
    17341 tgattgttaa cgtacctgaa ctaaacggat cagatgatca atactttaat tatgatgtac
    17401 atgtctatcc taaaaactat ttccaattaa actcagcaga gcttgataaa aaagacgaag
    17461 atggaaaggc tcttgccgga actgtttttg aattgcgaaa agatgatgga tctgatcagg
    17521 ttgttggaac ttataaaaca gacagcacag gtaaattaac tgtggatggt ttggaattag
    17581 gtgactatta tttcctagaa accgatttgg gaacagatgc taatagaaca acctatttac
    17641 tagatggtaa aaaattcaaa tttagcgtta aacgtgggga gactgctaaa gtagttcttg
    17701 atgttaccaa ctacaaacga cctgtaatta ctaaggtaat tgataaagaa accgttgata
    17761 ctggcgaaga tgttcaatat acattgaaac catcaattcc tggtaatatt ggtaattacg
    17821 aaaaatatgt tgtcaaagat gttctaggtg cagagttaac ttatgattca tcagaagtga
    17881 tcgaaggaat gactttagat actgattaca gtatcgaacg tgacggacaa acgacaaact
    17941 atgtctttac tgaatcaggt atggataagc ttcagaaaca atttgatgca ggggatcgct
    18001 tctttacaat tttgatgaac gctcatacta atagtaaagc taaaatggat acaccactta
    18061 agaatgatgt ttcaacgatc tacaacaatg gatatgaacc cgaagacgaa acaccagttg
    18121 atcacgctta tacaatcatt ggtgatcacc aattcattaa aacagacgga gatacaagca
    18181 aagcattgag tggagcaaaa tttgtagtga agaacaaaat tgatccgaat aaagttactt
    18241 acctaaaaca agatccaaca acgaaagctg ttacttggat tgcagataaa tctaaagcta
    18301 ctgaattcgt ttccggatca gacggtaagt tccaaattga aggtttgcaa tatacctatg
    18361 atgtagattt tgataaacaa ggtaatccaa caatcacaga taaaaaaatc aatgattatg
    18421 ctcttgaaga agttgttgct ccttcaggat atacaccaac tagaaacgat gttccatttg
    18481 tggtatcgaa tgatgatgct tctagcgaag taacagacat caagaacctt aaaacacctg
    18541 aaacagttcg tacaggtgga attggaacag tagtatttct agtggttgca ggtgtggcag
    18601 gaacagggct atatgttgtc tacaaacgag aacaacacaa agcttaatga aataaaattc
    18661 cctagagtgt ttttggctct agggtttctt tttaaaaagt gtgtgaaaag taggtatgag
    18721 catgaaaaag aagaatatca agttgatcat cttaaaaatt gggatgttgc tatgtttagg
    18781 agtaggtcta tatcctgtaa ttactcaggc ttatacctac taccaaactc aacaaacact
    18841 aaactcctat aaggaaaaat atgatgaagc ttctgaaaag gagaatgaag aagtcttcaa
    18901 gagatattca gaagttaata ggaaacgaaa agacacagaa caaacctcag tcacgattcc
    18961 tgaagataat aacaatattc cgtcaaatgg aaatcttcag aaggataaat taggtgaatt
    19021 agtgggtgaa attcttatac caaagattaa tgtggatttg ccaatttatg atggtgtaaa
    19081 cgatttccaa ctatcagcag gagcaggaat attaaccgga actgattatc caactggtgg
    19141 gattggaaac cattctgtta tatcagctca ttcaggcatt cctaataaaa aatattttac
    19201 agatttagag ttgctcaaac ttggtgataa attttttatt gatatttcag ggaaaaaact
    19261 tgcttatcaa gtagatcgga taaaaacaat tctaccaacc gaatttgagg atttgcttcc
    19321 tgtggcagga aaagattatg taacacttat gacatgtaca cctgttggag ataattctca
    19381 taggttactc gttagagggg ttagcatacc ttaccaaaaa agtattgttg agaaagaaaa
    19441 gaagaaagaa gatttaaagg taaaggcgca gatgaactgg tatcctatcg tagttgtagg
    19501 ggttttatta ataatagctg tgatagtcat tattaaaaaa atagtagcaa agagacgagg
    19561 aatgaaaaat gaagaataaa atactacttt ttttgactct tatttgcact ttggttttaa
    19621 caatttatcc tgcaaatcag ctctatcatc aattcaaaac aaaagagaat gtggcggaaa
    19681 tccaagagaa aattgagaaa aaacaaactc cttcaaaaga ggaagtggca aaaaaagtcg
    19741 aacaaataaa atcgaacagc ggtaaccaag gattagacaa tactcagaaa acagataaag
    19801 gagtagacaa tactcaggaa acagacggtg gatcatttga tattcgtgaa gctgaaggaa
    19861 aggtcaatcc atacacagga gatgtttttt caaaaaatca aacgaaagat tttgaaccat
    19921 ctacctattc aggagcggtt ggttatgtct atattccaaa aataaatgtg ctagagcttt
    19981 tgtttctagg cgcttcacag gagcatttat atagaggtgt ggcacaaatg agtgctacta
    20041 gtctaccttt atcaggtaaa ggtcatcaaa cagtaatagc aggtcatcgt ggcggttata
    20101 cgtcaccaca atttctttat gttgatcaat tagtagccgg agaccacatt tatatagatt
    20161 atcttggtga aaaagcagtt tatacggttt tccgaacaga cgttattaga aattacggtc
    20221 agaattcaac actgactgat attcctgatt tagatatgtt aacactttat acttgtcatc
    20281 catatcctca gaactacgat cgtttattag ttagaagtct aagagatgaa aaagcaaaat
    20341 acatcacgcc acaagtaaca gttggcaata ccgcatacgc tgatgccatt gatagtgaat
    20401 ccgtttcaga taagccagaa actgttacat cttctaatga tttatttgag gatgttgatc
    20461 gtgccaacaa ggaattagat ggaaataatg atcttaatat tccggaacaa ggtagcgtag
    20521 agatgcaaga cagaacgttt gctgaaaagg caagttcaat atttacagga attaccaata
    20581 aacatcagaa aatgctttta gtcatttgga ctgtattagg agcattagca agtctaaagg
    20641 ttcttattct tttaatcaaa gagtttagaa aagcataatg aagattataa aaattgatag
    20701 agtacagtaa aaagggattg atactattat tgatctcttt tttattttga atagagctag
    20761 attcactttt aaataaaata aaaactgatc ttaattccaa agaaaaagtc aagaaaaaaa
    20821 tacaacaaat ttttttatca gaatatttat cagaatatta ttagaaaaga taaactcaaa
    20881 agcttaattt aatagggtta ttggttgtat ttagaaataa tgtatggaaa agctataaag
    20941 caatagataa gatgtagaaa atcttatttg tgaatgaaaa acaagtcatc gtaaaatatt
    21001 aataggaggt gtagtaatgg tggaaatatc aaaaacagct ttagactcat tagctaaact
    21061 atttagtagc aggtttgatg gtgcagaatt tgttgaatat ctaagccaaa acgaaagtgg
    21121 aaaaactctg atcgaaacac tgaaaaaaga ctatttgaat gaaaatgact ttccgacatg
    21181 gacgaatatt gaagatgcta catgggatgt agtgtgtacg atatattctc cttgggtaac
    21241 gattgctgat tttgttttag ataaaattgg aggggataca taccctgatg ccaaatctct
    21301 tgctacaagt gactttaaaa cgttctatgg ttatctaatg ggtctaatag gcaatggaat
    21361 agatttgaaa gttacgccaa aacaaacacc tgaagaaatt gaaaaagaaa aaggagaggt
    21421 tcaaatggct tatgcagatg acagagaaaa agtaaaatta ttggaaggta aattatctga
    21481 agaagaactt ggaaaaatgg tgcaagaaat caatgaagac agcaactatt ttcctgaatt
    21541 gaaggtatat caaaacgacg aagatttctt taatgaaatg tttagtaata gtccatggga
    21601 agcagtgagt aaaacaaatt taggagaatg gaactttcaa gatgatctag ttgcatttag
    21661 caatgatggg tatttacgat catattcaga aagtgagtat agagctgaat taaaaacgta
    21721 tgaaacggat attctgatga gctatttaaa tctattagag agtggtcagg gagagttaag
    21781 agataaagtt gaagaactat taaaagaaga gctataggga ttatttcaac ataaacacaa
    21841 gcagaaaact accaaacaaa acgtaccatg caaaaagccg gttaaaccgg ctttttgctc
    21901 tattatgaga ggagatcaaa ccatgagtgc aacaaccgat atggctttag aagcagtaat
    21961 aaaagataat acaggaaatg tcgaaaatgt agaagacttt agcgatattg caatcaatct
    22021 ttcagcatgg atttcagctg taggagaaga aaatatcgaa tttgagatca agaatatgga
    22081 tgacttaaaa gcaaaatttc cttttatcgc atcgaaacta agtcaatttg ccgaggagaa
    22141 atcgtggaat gaattagtta gtttttccga aaaaatttat gatattacgc aagaagatga
    22201 gtgtgtttca tgggaaatga ataatcaata caagaaaatt gatgctttcg tcaaatcacg
    22261 tatagaagga acagcttttg agtttgaagg tatagatacc gcagataatt ggttaaataa
    22321 agaatccttt attaaaagta tagcggatga tcttgaaaaa caatggtcta aggaagacta
    22381 tgcagagtac ctaaaaacag aaagagatac agaggtgaaa gaaaaactca ctgaggtatg
    22441 tttggatgaa catattgatc ccaatatcgt aaaacaagaa gactatgaag tggttattga
    22501 taaagttcct actgattttg aggaagttgt aaagtatata gttgaagaag cattatcgca
    22561 atactcttca gatgaggaag tggacgaaga aactgttcgt accagagttt ggcaactggt
    22621 ttttgatgac aaagattata aatatttgag tgatcaaaac tcggttaatt ggagtgtacg
    22681 attattaatt gatcaaaatg atattgaata aaatagagct gaaaagctct attttttttg
    22741 ataaaaaaac tagctatttt tttaaaaata atttggttca aaatttattt ttttaacttg
    22801 ataatttttt gccatccttt tttaaattgg ctatatatca aggtttatag tggggataaa
    22861 taaaaataca tttgacaaaa tttacaatat agtttattat atatttatct tattatgata
    22921 acacaataaa aggaatgatg gtatgaaaaa gaagaagttt agtcgatcgt tgttaggaat
    22981 tttagtttta gttgcggttg gtcttagtgg caagggttac tttcagaata acagcactca
    23041 ggaacatgat tttcagatca aaagtagtgt tcaggtaggt aataaagcag acagtgaatc
    23101 aaaagaattt ttagggatac gttatgatcc aaacaaatat ccaaataata tcgtaaacat
    23161 taatgatggg aaatatacac tgacaaaaga agaccatgaa gcattatcag aaaaaggcag
    23221 tcaaaccttt tgggtaaatt atgcagattt agatcaactt ggaagagcag gagatgttga
    23281 agcgttgatc acctatgatg ctataaattc tcactcatct aaaaatgtgg aacgtccatc
    23341 tttcaaaagt tctgtccata ttgcaggaga atacaaggat ggagtatttg atcaagagag
    23401 agatacttgg gtcggcaaag attcaaacaa tgaaatcact cagctagaag gatataaagg
    23461 ttatctctat aacaaaagtc attcattagc atgatcgcta ggaggagata tggaggtaca
    23521 caatttaaca cttggaacga gagcgcaaaa tgttgggaaa aatgatcaga atggagggat
    23581 ggcttactca gaaacgattg tacgtgatgc aatctataaa aataagaatg aaaaagtatt
    23641 gtataaagtt gatccaattt ataaagggaa tgaattggtt cctcgtggtt ctcatgtcga
    23701 agctttctca gttaacgata atggaaaaac ggtaaacctt aatgtttatg tatttaacaa
    23761 tcaaaaagga atcaacatca actatcaaaa tggtacttgg gtaagagatt agaaattgag
    23821 aaggtgaaga attggaaaca ataagcatca atggattaga taaatcagtt gcaaatcgaa
    23881 taaaaaaagg aaatggctac tatcttctga agaagggtaa atattcagtt gctagagagt
    23941 tagaaacaat gagtatcgtg tatgtagcta agaatcattt tgacgtagaa caggctattt
    24001 ttgatccaga ttctgaaata gggaaatgtg ttgaattatc agagaaaact gttagtacaa
    24061 aaggggcgat gatcattgag agagaaatgt tagacgaaat cagagtaatc attcctaaag
    24121 atacgcagat gtgtgaggac agcataagtg acgatgaacc aaaatcagaa agaaaataag
    24181 gagatattca gatgagcgaa aaatccattg aattaaagga atataagcga agcgatggag
    24241 aaaaatatta tcctgtagcc agtttttcta aatcagtata tctggttgga cgtgcattgg
    24301 tagttggtgc aattggcata tatattttgc attgggtaac aaaggttgta caactgcttg
    24361 tagatacctt agtaagtatt cttgtctcgt taaatgatgt taacggaaca gatgatttag
    24421 gattaaaaga aatgtttaaa gagttactca atgcgttttt tcccaattat ttaagttatt
    24481 catataatgt acaaagcttt tttgcaaagc tacacttggt agacatctct attttagtat
    24541 tagaagcagg aacggtattc tttgttggat atttggttac tttgatgttc aggcatcact
    24601 atggagaaca aagaccattt tttgatgata tggaaagtag aaggttgaaa agagaagctc
    24661 tagaagctat ggatgtgaag tatgatccaa acaagattat aggagatgaa acgaacaaca
    24721 aaaaaagaaa acgaaacaaa acgcaattag aatccgatgt gctatcaacg cttaaaaata
    24781 tcaaaatttc tgttcataca agacgtgatc taagtgtcaa tgaagatatt cgagaatata
    24841 tcgtaaaaat cccgcaacca aaagggaaga aagttagaga aacagtttta cgacaaattc
    24901 aagatttagg gtcgatcctg acaagagcaa caagcggacg agccaaattt ggtgaaaaag
    24961 aagatgaagt ttcaggcgat tatttcattt tccgagcatc agtaaatatt gaaagagaac
    25021 gtaaggaaag agtaagtacc aaaaacaaag aaacggaaag agcaacatct tcaatcgcag
    25081 tttcaaattc acaattttct tttccaatca atctctttgc cgataattcc aaaaatatcc
    25141 aaaaccaaac cgaaaaagct agaaaatatg ccgaaactca aaagcaatct ttagaacttt
    25201 atctatcttc tgctgacgta cctgcaagtc taacagatat gcacgttggg aatacatcta
    25261 ttgaatattt gtttacttta tctcgtacca gaaaaattca actaagctca atgaaagaag
    25321 aaattgaaac ttacttgcat atatcaggtg taatcgtgcg gatcaacgca gggcgtattc
    25381 aaattactct accattagga gaaaatgatc gtattccaat tgatgtacca tcaatgatca
    25441 atagcgtgtt ttaaaagaca tcacaaatag aaacagaaaa tacaaactac cagttcacaa
    25501 acagaaaagt accgagaaaa gtgaggaata gaaatggcta tgaaattatt agagaaagtc
    25561 aaaaagagag ccgaaaaagc tgaatctatt ttgctagata aagaacctaa tataaagctt
    25621 aatggaaaat tcagtgtcaa tatgttgaac acaaaggcta aagacccgac agatgctatt
    25681 tttggaatta agcctgatgg cacaattgct catttttgca tcagcgaaat gccacacatg
    25741 ttggttgcag gaacgacagg atcagggaaa tcagtattac tgaatgaaat tcttgttaca
    25801 gcaatgtgtc actcaacgcc tgatgaatta aaaataggaa ttgtagaccc aaaaagggtt
    25861 gaatttggaa gatacaaaaa attgccgttc atgttggctg atccaattac agatatggat
    25921 gaagcttatg atttttttga atatcttgtg atcttgatgc atgaacgtta caaactgatg
    25981 gaaaaagcag ggttacaaaa catcacgcta tataacgaat atgccgaaaa aaatggatta
    26041 gatagatttc cctatgtgat cttattagtt gatgaatatt cacagttggt tggaacacat
    26101 aaagaagtag aaggtttgat tgttcaatta ggacaaatgg caagggcagc aggtattcat
    26161 gttatcttag ctacacaatc tcctcgatca acagttgtca ctggtattat caaagccaat
    26221 tttccgtcac gtgtttgcct tatggtagca agtgatttag aaagtaggat cgtattagat
    26281 gaaggcggtg gagaaagcct aagtccaaag ggagatatga ttattaagct cgttaatgga
    26341 agtatggtta gagcgcaggg agcttacatc agtaacaaag aaattgaaac aattttcaat
    26401 caccttagaa atactatgcc agaaccagaa tatgttgatt accgagcaat cgtagctgaa
    26461 tctcgtggtg agggtggaga agacgaggat tttggtggaa ttggtaaagc tcctgtaaga
    26521 aaatctgcta aaagaaattt gaacacacct ttagcaaatt catcatctaa agagccatca
    26581 attgaaaaga agagtacgtt tgctctaaaa cttgaaaaaa acaaagaaga aaaagaaaaa
    26641 caaggagcta aaacactaga aaaacttcaa gccgaaaaat tagaaaaagt tttaaaagca
    26701 ttagataaag ctgattctga accgcaaacc tcaaacaaca cgaataatga acagccgatt
    26761 tcatctaaaa ctaaaccgaa aactactgat ccgaaagttt cagtaaacgt gaccatcaca
    26821 accgataagt ctgagaagaa acaagtaaac ggaacgactt cacctatcat gcaaaaaatg
    26881 ttgaaagcca aaaaacaggt gcataaatga tcattgtcag ttcttttttg aggtggctta
    26941 cgccatataa atatacagat gaagtagttg tgaaacgaac gaagaaaaga gataaaaaag
    27001 cggtactaac tcctaaacag atgcttgatc atggcatttt aggggtgaag gttttagaag
    27061 tgaataaacc gtttgaatca aatagtgcaa gagaaaagtt gcttggagct gaagccgatg
    27121 tcgcatattt ttaccatgac aaaagcaagc atcaaatagc tattaaattc aatccaaaat
    27181 cggcatggcg gtatcaagca agagatgact tgaaacgtag gaaacttaga ccaatgctct
    27241 atccagaagg aaaagaagca tttgatagaa cccacatcgt tcccattggt tttcatggta
    27301 cagaatcaga taaccgccta ttagtaggtt ggaacagcaa tcaaaataga gttcagatca
    27361 ataaatttga gaagaaagta aggaaattca acgatcgaaa aacgatccta tggtttgcag
    27421 atatttgtaa acagccggat ggaacagcaa agtgggaagc ggtgatattt gacgaaaatg
    27481 gtaaaaaagt aatgtctgaa acatggcatg ataaagctaa atttgtttgg cagtagcagt
    27541 agaggtgaaa aagattgcta gaactttttg aagcaaatag aacaggcttt aatcaaaagt
    27601 tagaagtggg agaaactata gaatataaag ataaaaacta tattattatc ggaatctata
    27661 acacgagaat taacagaata ggtgatccac gtataaccag tgacttggtt tgtcaatctg
    27721 ttaactattc acctgatctg acaagcaggt ataagaaata cgtgttatta gaatatagac
    27781 acaagattac tgatgaaatt ggtgtaaaca acacacgaaa catatttaag atcggaacag
    27841 tgattcctta tagtaacaac gaagaaaaaa tattttatca gatttacgga atagaaaagt
    27901 ttgaatatga acatgtcgat ctgctagtga cgtaccaaat gcgtttaata gaaccttggt
    27961 cacaggcaga aattgataaa gcagtaaaat tgaaccgatt atcaaaattt aacgtattag
    28021 gaaatgcctt ttaaaaatcg caatacacaa acagaaaaat accaaaaaat tagcaatgaa
    28081 cgtaggtaag aacatgacaa taaataataa taaccctaaa aaatataatg ctcttgtcca
    28141 atgctatatc gaaaacaaaa ttacaatgca agaagtcgct gatgaattag gcaaatcaaa
    28201 gagcctagta tcacacgatt ttaagcacaa tgtttttccg aacccgattg agctgaggaa
    28261 aaaatttcaa aagagagttt atcaagaaat aggtaagtgg attgatgaag ggaatgcaat
    28321 tgacccttta attgaaaaat atcctttttt aaaaagcaaa aataaaattc aaaggttaat
    28381 tcgtcaaggc gatattcaag ctaaagaacc tgttttgaca accaatgatt tggctaaaat
    28441 aaatcttcat aaacaaatta ttgaagactt tgaaaaaaat ctttcaacaa ctgaaatttt
    28501 aaaaaaatac aatattccga gaggaaagct aacatcgact ctttttagag aatatggaga
    28561 atcggcagtt actaaagcaa aggaagtagc gaatgagaga aaaaaacaaa aatagtagtc
    28621 gatacctagt agcgactgta gtttttgttt gtgtattgat tttcctgttt gattttttgc
    28681 agattggaaa aagcataatt ggaaatacat tgagtgcaaa ggaaatggtt gctttaggtg
    28741 gtttgtatga aggacacttg ttcccgaata tattcatttc tatcgtttct catattaact
    28801 tgtaccattt aatttctaat ttattgttct tagtagttct tgaagaagca ctgaatgatc
    28861 agtatgagtg gttaacatta ttgatcgttt taatcatatc aggatttcta ggaagcttag
    28921 ttccttttct gctacacagc aacatcgtaa gtatggggct tagtggaggt atttttggat
    28981 ggattgcttt atttgcgatt gcatacatcc gttttgatag caagaaacat ttattactaa
    29041 gtgtttttgt attattgata atcggcattt tactggtatc tacttttgaa tcaggcgaga
    29101 caaacgtctt gtcacatgta atcggatttg tctgtgggat gatcttggga tttattctaa
    29161 agaacgtttc attgatcaaa caaaagaatt aaaagggagt gctaaaaatg ccaaaaaaaa
    29221 aagaaaagaa aattccagtg tttttgtctg tctcagaaat gttatcactt caaatactgt
    29281 taagaaaaaa gattgaaaca atagacgaag aagttagaaa taaaagggct atgtacgaac
    29341 aacgtgaagc gctaagagaa cgaatccact attttgatga tatacacact aaaatctatg
    29401 aagcacgtac aaatcacgaa gacgaaagca aattaggatt agctcaacaa cgaatatttg
    29461 aatggctgaa tacgaatact gagcaagaac cgttaagagc aattagtaat ctttttgaat
    29521 ttggaacgaa gcattggttt aacgctgtag aaaaggatgc tgacttacaa gaagcgttcg
    29581 atacactaag cgaggatagc aaatccatgc gagacttatt gatcacttat ctaaatgagc
    29641 atgaatttga acagacgaaa gttaaaaaga aaaataaaaa atagaaagtt gtgatgtatc
    29701 atgctgttta ccttattagg ttatttatgt ttagttcttg ctttttttgg agcaattgct
    29761 atatttgtcg gtcagtttca agaaaataat ccgataatgc gtaacatagg cattggaatt
    29821 gttatcatat ccttgattgt atttggtgcc acaaagattt ttgggagtaa tgatcagcag
    29881 tttggtattt ctggagttga taatagttat gctccagatt cattgggaag agtagtgata
    29941 gatggaacgt ttgagaatgt aacgagctac aaaaatgttt cattgacagt gaaagaagat
    30001 gacaagaaag catcagatga cgttctcgct acgatcaagc cgtctcaatc acagtttgaa
    30061 gtgttttata cagtcaaaga gaataaccct gtacgcaagc taacattagt ttttaacgtt
    30121 gacggagaag aatactataa agatgtaaaa atcaataaag catcaacgaa aaagattgtc
    30181 gaatcagcga aaaaagaagt agctacagaa gaaagtacaa ctgaatcaag ttctactgtc
    30241 gaagttgaac taccagataa aattgatttt gatcctgaaa aagtcaaatc gtttgaatct
    30301 gtttctggtt ctatggcaga tcggtttaac actgaagttt tcaaagctat caaaactgtt
    30361 aaattggata ctgaaaagca aaggattaat gttaccttat tcaatgcagt tgaatctatg
    30421 gatcaggata aaaaagttac ggttgcaaga gaactaggga aaagagtaat tggattgtat
    30481 cgaggtacgg caaaaccaga ttcagacggt tcagatttgc cagttcacgt agtttatgaa
    30541 gacggtaaag tttttggaga ctctacagca aatgaagcac taacgattaa attagctaaa
    30601 tagccggaaa attataataa acaagtggtg tcacaccacc atcgcatagg tgtgacacct
    30661 tggagatgat tactgatggt agtaaataaa gaaaaagtaa gaaagtatgt tactttaaaa
    30721 atagcttctg ttgaatattt agaaaagcga gctaaagaag aagaaaaaac aagaggtaag
    30781 aaggtaacag tttcagagtt gttagaagaa ttaattgaga gtgaccaaac aataagacgt
    30841 gcatttacca ataggtctgt taaaagttag ggtgatacta tctttaattt caaccaaatt
    30901 tctaatatat gatgaatagt ttccttgtac aaaatatcta attaagagat aaaagttagg
    30961 aggattcatt caaaatgatt atccaaaagt ttgactggaa aatgaaatta ggaattagtc
    31021 ttgtaatatt gggatttatt atttttttgg ggataaaagc acaaaaaaat caatctactt
    31081 ttgaaactat gtcttctcaa attattgagg aatgttccaa aatttattat tgtgagaaca
    31141 aaactgaaga atctaatggc ttttattata tcgatcaacc acatgatgtt gttataaaaa
    31201 tggagtcaca tgaagtaaac gaatggattc aaagaagtac taatgtatca aaaaagttag
    31261 aaagatattc tcaaacgttt ttaagtaaag tcattttttc aaattgcaaa aatgaagaaa
    31321 cgtctcaaag agtagctagt tctttagaaa atttaaaatt ttctgtttca aaaaaagaga
    31381 aatttgtaga tttacgaaaa taaactatcg aaagtagtat gattatgatc ttaaaatatc
    31441 atcttaagag aagaattacg attgtttata ggatttacaa aatgaataag tttgaagagt
    31501 gatttggtcg cagactagat cactcttttt gtgtaaaaaa actttacact tatttttgag
    31561 tgtgttaaga ttttttatat agagtgacgg tttcttgtca gagtggatga taaccatcaa
    31621 tattggagga ataataagtg gaaaacaata atgatattag atattttagc attggggaaa
    31681 gagttacaac agtaagtgaa ttatcaaagg attatagaga taaagaagga acgattattt
    31741 ctcttaaaaa gaatcgaaga ggtgtttggc aatatgttgt tgatttaggg gatggcgtta
    31801 cagttgagtg taaagatagt caactgatca gaaatgatgg cagtcattcc attgaaaaag
    31861 caattcttgt attcccaaaa gtatcaagca agattatgaa tgactggttg cttggaaaat
    31921 ttggtgagta caataaaaaa gaaatcgcag aagtgatcgg caaatcttat ccaacagtat
    31981 tagggtatct aaaaaagata acgaacaaag aagcgaaagt tgaggatgtt ttaacagtaa
    32041 gagaacttca gtcattaatc gaatatcgga ataaacaaat aacaaatcct gagtacctta
    32101 aaaaagaaga tggtctattg aaacaattgg aacaggtaaa taaaatccgg aaaatgcaaa
    32161 aggtagctcc tttaagccta gataacttta aaaaaatcta tttgaaagag atcgaaaaag
    32221 actaatgtgg agtgtcactt taccgtttca acctgtatct gcaccaagac caagtgcaag
    32281 aaggaatgtt gctgatgcaa ctaatgacgt agcagagaaa aggatttcaa cctattatga
    32341 taaaaagtat ctcgattatt tgaaaaaaat aaaaagcttt atcgaagatc aaggtttgtt
    32401 agatgatgaa ttttactcga tcgtaaatga tccgatgggt gcaattatgg aagtggactt
    32461 ttactatcag ataccaaaaa gctataaaaa agtctataac attatgaaaa cgacagcgcc
    32521 agatttagat aatttagtaa aaagtgctat ggatggtatt ttcaacatct cagctatacg
    32581 agatagccat atcacagggt taattgcttt taaatacaat gtggcaaacg aaggaaaaac
    32641 agttgtgagg atcaagcgat attcagaatt tgagtgggat acttctgaag gattgaacgg
    32701 agatatttgg gaagccacat ttgatttcaa accagtagca acgccaagac caaagtctaa
    32761 atttagagga aatgggatta tcacttacta tccaaaagag tataacgact atttggaaaa
    32821 tatcaagaat acgcttaaag agaaagattt ggtaaatgat caactgaaaa aggtaatgaa
    32881 tgctcctatg ggggttatag ctcagataga ttatttttac caagtgagaa aagataagaa
    32941 aaagcttgat tcactgatgc gtacttcaca gccggatatt gataatttag tcaaaggaac
    33001 attggacgga atttttaaca aggaaatagg catcaaagat agcagggttg tagggttaat
    33061 tgcttttaaa tacaatactt ttgaaaagtc gaaaacaaac gtgagactac aggagatggg
    33121 ctgatgaata ataatgaaaa aattcttcat gtacttgata gctttgaaat aattcaagaa
    33181 gaactaaaaa aatatcggga tgttttggaa caaagatatg attttgtcaa tcagcaaaag
    33241 agtaatcaca tggatttcat tctcaatatg aatgacttga agaagaaatt agttgaacga
    33301 aaagaacaag aaaagttgat caaagcttac tttgaactcg gtgaaaaaga agttaaaaat
    33361 gcaatggaac tgaatgaaga gcgcagggtt ttagatcagc tgttagaaca attattggtg
    33421 atgtttcaaa aaggacgaat agatgaggat ttaattgaag aaggattgag aaaatatcct
    33481 tcaaactcag gtatcggaat agtgctgaaa gcgattgatg aagaagaaat tgaagctttc
    33541 ataccgccag aggattttga atcggcaatg gaatatataa aatattatag ccaagggatc
    33601 actgctttta gagagtttga tcctgaagat gttattcatg acttgaacaa cctaaaagaa
    33661 tggtgtgaaa gttatggagt cgatgattcc ggattggact acttgatcag tattatggaa
    33721 atcgaagaag agatgccgga taagcctgat ccaacagata ttttagaatt gatccatgaa
    33781 gctagaaatc caattgccta tatttctaga ggatatactg tattggaata ctataagcct
    33841 tatatatcag cgatgaacca tttaagacgt gtattaaggg aaaaacgtga ataccgctca
    33901 gtgttgaatg caacgaatag attagaaaaa gctgtttcag agctagatgc ttattatcgt
    33961 gaacattacc tgcaagcagg tggtatgcca agaaatacga aagcaaatat cagtaggtat
    34021 ataaaaaaag ctgagtaggc ggagttagcc cataaaatgg caaaaggtaa cctgaaaggg
    34081 gcattttgaa tgaaaaggga tgtttttgaa ttgtacgtat tagaacgtct agaagacggc
    34141 atgatggcac aagagataat tgatgaaatt gatgatatga tcagcagatt tggtcaattg
    34201 gagttgaatg atggaaacaa aaattcggta gtgctaaaac aattttggtt gaacaatttt
    34261 tcgtataagg attttatcaa agaaaaggca aatacattgt actgactaaa ttcacgagga
    34321 gcagtgatga gagcttattg ctaagagcag ttaggctctt ttttgtttgt gtaaaattat
    34381 ttgttttata atacacattt atacacaaaa aatattgaca gatagtacac aaatgtggta
    34441 ggatatattt gtaagcaagt tagattaaca agttaaagga agagcagtta tgaaaaaaga
    34501 agaactaata gatatatttc aaattgttga acgagcaaat aatatgggaa ttatgttttt
    34561 tgatcgtatt tctttaaa
//
LOCUS       DACGLH010000025        33288 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.027, whole genome shotgun sequence.
ACCESSION   DACGLH010000025 DACGLH010000000
VERSION     DACGLH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.027"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            134..409
                     /locus_tag="IXT21_001292"
     CDS             134..409
                     /locus_tag="IXT21_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ4868705.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="IXT21_001293"
     CDS             415..1182
                     /locus_tag="IXT21_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ4868706.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="IXT21_001294"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="IXT21_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ4868707.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="IXT21_001295"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="IXT21_001295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ4868708.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="IXT21_001296"
     CDS             5326..5628
                     /locus_tag="IXT21_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ4868709.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="IXT21_001297"
     CDS             5631..6794
                     /locus_tag="IXT21_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ4868710.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="IXT21_001298"
     CDS             6820..10248
                     /locus_tag="IXT21_001298"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ4868711.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="IXT21_001299"
     CDS             10269..11393
                     /locus_tag="IXT21_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868712.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEIMSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="IXT21_001300"
     CDS             11383..11820
                     /locus_tag="IXT21_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ4868713.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="IXT21_001301"
     CDS             11804..12142
                     /locus_tag="IXT21_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ4868714.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="IXT21_001302"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="IXT21_001302"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ4868715.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="IXT21_001303"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="IXT21_001303"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ4868716.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="IXT21_001304"
     CDS             13299..14342
                     /locus_tag="IXT21_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ4868717.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="IXT21_001305"
     CDS             14501..15229
                     /locus_tag="IXT21_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ4868718.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="IXT21_001306"
     CDS             15345..15590
                     /locus_tag="IXT21_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4868719.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="IXT21_001307"
     CDS             15587..15805
                     /locus_tag="IXT21_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ4868720.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="IXT21_001308"
     CDS             15941..16480
                     /locus_tag="IXT21_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ4868721.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="IXT21_001309"
     CDS             16473..18746
                     /locus_tag="IXT21_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ4868722.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="IXT21_001310"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="IXT21_001310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ4868723.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAVQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="IXT21_001311"
     CDS             complement(19919..20173)
                     /locus_tag="IXT21_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ4868724.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20573..20842)
                     /locus_tag="IXT21_001312"
     CDS             complement(20573..20842)
                     /locus_tag="IXT21_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868725.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21312..21767)
                     /locus_tag="IXT21_001313"
     CDS             complement(21312..21767)
                     /locus_tag="IXT21_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ4868726.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21905..23833)
                     /locus_tag="IXT21_001314"
     CDS             complement(21905..23833)
                     /locus_tag="IXT21_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ4868727.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24030..24863)
                     /locus_tag="IXT21_001315"
     CDS             complement(24030..24863)
                     /locus_tag="IXT21_001315"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ4868728.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25055..25759)
                     /locus_tag="IXT21_001316"
     CDS             complement(25055..25759)
                     /locus_tag="IXT21_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4868729.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25782..26408)
                     /locus_tag="IXT21_001317"
     CDS             complement(25782..26408)
                     /locus_tag="IXT21_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4868730.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26734..29109
                     /locus_tag="IXT21_001318"
     CDS             26734..29109
                     /locus_tag="IXT21_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ4868731.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQIAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29133..29624)
                     /locus_tag="IXT21_001319"
     CDS             complement(29133..29624)
                     /locus_tag="IXT21_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ4868732.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29811..30731
                     /locus_tag="IXT21_001320"
     CDS             29811..30731
                     /locus_tag="IXT21_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4868733.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30885..31229
                     /locus_tag="IXT21_001321"
     CDS             30885..31229
                     /locus_tag="IXT21_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ4868734.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31419..31919)
                     /locus_tag="IXT21_001322"
     CDS             complement(31419..31919)
                     /locus_tag="IXT21_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4868735.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32447..32530
                     /locus_tag="IXT21_001323"
     tRNA            32447..32530
                     /locus_tag="IXT21_001323"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32481..32483,aa:Leu,seq:cag)
     gene            complement(32854..>33288)
                     /locus_tag="IXT21_001324"
     CDS             complement(32854..>33288)
                     /locus_tag="IXT21_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ4868736.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11011 a         5771 c         6783 g         9723 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga ggaattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat aatgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccgtgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta tataacaaag
    20461 agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt
    20521 ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa ggctattctg
    20581 gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt tttatgacca
    20641 actggttccg acggttccat ttgccactta cttccagcac atatttattt aggggcaaac
    20701 gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca cggtcctcaa
    20761 gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac gtatcaatta
    20821 aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag
    20881 gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt tcttttgtcc
    20941 actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag agcatcgaat
    21001 tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt aaaaaaagcc
    21061 cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa gcagtatcaa
    21121 acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc aaaagtaccg
    21181 ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac attgtatcac
    21241 ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc ctaaaataga
    21301 caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt gataccagaa
    21361 gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat cgtgccgaaa
    21421 acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt ggaagataag
    21481 acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt aaaggcttct
    21541 aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc cgctgtgatg
    21601 atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga acgggaggct
    21661 ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg atttttagct
    21721 aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa atatattcct
    21781 cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag gaattcatcc
    21841 ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata aaaaaacact
    21901 ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta catttttttg
    21961 aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga ataaccaata
    22021 ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc aggaagttag
    22081 aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg atgatccgcc
    22141 gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt ttcattaaga
    22201 cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca cctaaagaag
    22261 cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt acttggtaaa
    22321 gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt gcatagtaat
    22381 gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg ccggttatca
    22441 tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta tttgcttttg
    22501 gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga taaatcacgg
    22561 ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg attatcatcg
    22621 tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag gtagtttgta
    22681 atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt attgtgcaat
    22741 cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa
    22801 gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca actggttttc
    22861 ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa agcttttcta
    22921 aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga gcagctagag
    22981 aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat aataccattc
    23041 ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga aggatcatta
    23101 gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg ataaatgaag
    23161 ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta gcaaagacag
    23221 tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc agcgttcctg
    23281 catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt aatgcagact
    23341 catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct gctcctttag
    23401 cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc atttgtccat
    23461 tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta atttcttttt
    23521 gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg aatgtcaaca
    23581 ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa gccatcaata
    23641 aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat gtttgtttat
    23701 atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga gagaatcggt
    23761 cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca aaagcttttt
    23821 tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata ataaaaatgt
    23881 aattgataat aataacagct aaatgagaac gataatggtt atcatttggt gattaagctt
    23941 gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac cagaacattt
    24001 ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata accaatcaaa
    24061 atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt cttcaaatag
    24121 cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt tcctacaaga
    24181 agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt gacgatcttc
    24241 aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca
    24301 aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg ggatgttccg
    24361 ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta cgtaaagtca
    24421 ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat aataaaggca
    24481 ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt tgcttctaac
    24541 caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat cactttaaac
    24601 caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa tgggtttaac
    24661 ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac gacattgaac
    24721 atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag gatcaaatga
    24781 ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag aatcactgct
    24841 ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac atcttatagt
    24901 ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa cttgagaaat
    24961 aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag actttgcctt
    25021 ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg cgactatgtg
    25081 ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat cgaccgtttg
    25141 cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc tgtttaggat
    25201 cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa acatttccac
    25261 caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca cggtctcgca
    25321 atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca ttagcaattt
    25381 gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc aatacacgag
    25441 ggatcacacc ccacatttcc atgtcatatc caccttcaag agacccgaaa caccattctc
    25501 taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct cctagaataa
    25561 tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa aagtcgacat
    25621 tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg aggggtgtat
    25681 cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt cgtacgatat
    25741 aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt gaaaaaagga
    25801 tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata gactgcaaat
    25861 tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc taaattccct
    25921 gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga aacgacaaaa
    25981 tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac gacgcgaaaa
    26041 gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg cgagacaata
    26101 acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg atcacctagc
    26161 catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg taaaggttcc
    26221 actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg ctgttggttt
    26281 gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat gatcaaaagt
    26341 gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt taatctttcg
    26401 atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag tataacgcat
    26461 tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa ttatcagact
    26521 atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata gcattttaat
    26581 atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat atccctgcct
    26641 aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa ctaggttttt
    26701 tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa aatatctgtg
    26761 cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa aaaaagtaac
    26821 catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc gggagaaaac
    26881 tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta cctgatcgtt
    26941 gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga tgaaatcaaa
    27001 acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt ctttcatcaa
    27061 gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat aaatctgcaa
    27121 ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca tcagacaaga
    27181 gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg gcttgttttt
    27241 caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt cccccatgat
    27301 gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat ccgtacgaaa
    27361 cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac gttattcccc
    27421 gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac ggctaaaaca
    27481 ataactagac aaatagctga agatgctatg acagcattaa aagatacagg agctgaagta
    27541 aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac gacctgtaat
    27601 ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg tttatgggat
    27661 cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc aagaaagcat
    27721 acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt aatcattggg
    27781 gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga agatgaaaaa
    27841 aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg ttcccgagag
    27901 atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc tataccaaag
    27961 gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga ttcgatccga
    28021 gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc tgaatcttta
    28081 gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga atttcctgag
    28141 cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt tttgaaggtc
    28201 agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg cgatctgata
    28261 gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga taaaggaaaa
    28321 agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga aacactgggg
    28381 ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa ccagcaagtt
    28441 ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct ggcaaaatta
    28501 gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt tttctttcct
    28561 tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc cgacagcaat
    28621 gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt aacaaaattt
    28681 caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat tttttcagaa
    28741 ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt aggtttgccg
    28801 cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga acaccttgga
    28861 tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg tggtcgagtg
    28921 attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt ttctagtttt
    28981 tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg aagtgttggg
    29041 gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa tcaaaaaaac
    29101 gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa ttgcaactcg
    29161 attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc acggttctta
    29221 cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt ttcatttttg
    29281 atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa atcggcagtt
    29341 tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt tttgattggc
    29401 acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat tttgaaatca
    29461 tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt agaatccttg
    29521 aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt tacttcttct
    29581 gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac cctcctgata
    29641 tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga ttcttacctg
    29701 tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat aaactgttat
    29761 tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt atgactgcgt
    29821 atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga ttgaccgctg
    29881 cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg atgaatggga
    29941 tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata gacacaggaa
    30001 catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa gtaggaaaac
    30061 gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg atgctgcttc
    30121 cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt gttttattag
    30181 gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg gatatcatct
    30241 ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg attctaaatg
    30301 gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg tatacgatta
    30361 tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat caaaaagtaa
    30421 cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca aaacggatgg
    30481 tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa gggaacatga
    30541 tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt catgaaatcg
    30601 acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg tttgcaaatg
    30661 aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa aatcgttcac
    30721 aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca tctaataaga
    30781 acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca agcagctaaa
    30841 aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc gaagcaaacc
    30901 aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct gccttggaaa
    30961 tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt tatgatatag
    31021 aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact atgggctgtc
    31081 cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg ccagaagtaa
    31141 cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag atgagccgtt
    31201 atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat gagaagcaaa
    31261 taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa ggtttctttg
    31321 tttataaaat tttgtctgct atattataag ttaattaaat aaatgtgttc tattgataaa
    31381 ggacattcaa atccctttcc ttttggaatc cttctttttt acatcagttt gattttcgta
    31441 tagtattctt tctcatcttc acgataaatc gcagccccat ttttttgttg tgctttaaga
    31501 ctgccaagat tttttttatt tacagacaaa agtgcctcat caatgccgat tttttttgcc
    31561 tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt ttttacgata attaggggag
    31621 atgccgtaac caatatggcc agcgccgttt cttaatgttt cgttcagttt atggcgcaaa
    31681 ttaaaaattc ctacatactc atgttcatca ctttctaaaa taaagacggt tgcgggaacg
    31741 taactctcag gaagatcgat tcctaaagac gttttttcgc atttttttac ataatctaaa
    31801 aattcagcaa atgtatagcc ataagcattg ttgataaatc cactctcatt tggtccaaag
    31861 ctagtttgta gctgataagc tttctttaaa tctgcttccc atagtttgat cagtttcatc
    31921 gtattcacct ctttaagtaa ccttattcat ttgttttcta ctatagcact attgttgtaa
    31981 atagccatgc ctatttttat ttattgtgtc atatcgtatt aagaatcatc gaacaataca
    32041 aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt atgatctcta
    32101 gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag taatcaacag
    32161 aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag aggaaaaaag
    32221 aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa cattgaaatc
    32281 gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga gttgctcgga
    32341 tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag aacaaaaata
    32401 tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg ctatggcgga
    32461 attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag ttcgagtctt
    32521 cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga tgagaaagaa
    32581 aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc agattcaaat
    32641 aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat tgattcaaat
    32701 agtagattta tagattctga tacaagaaat actggcaaac gatagttaag gaataaagac
    32761 taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct cttactatat
    32821 tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg cttcccagaa
    32881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    32941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacggccgat
    33001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    33061 tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    33121 ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa caggttgttc
    33181 agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    33241 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DACGLH010000026        32377 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.028, whole genome shotgun sequence.
ACCESSION   DACGLH010000026 DACGLH010000000
VERSION     DACGLH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.028"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(410..769)
                     /locus_tag="IXT21_001325"
     CDS             complement(410..769)
                     /locus_tag="IXT21_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ4868737.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(799..1140)
                     /locus_tag="IXT21_001326"
     CDS             complement(799..1140)
                     /locus_tag="IXT21_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868738.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1137..1811)
                     /locus_tag="IXT21_001327"
     CDS             complement(1137..1811)
                     /locus_tag="IXT21_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4868739.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2987..4285)
                     /gene="asnS"
                     /locus_tag="IXT21_001328"
     CDS             complement(2987..4285)
                     /gene="asnS"
                     /locus_tag="IXT21_001328"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ4868740.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4325..5515)
                     /locus_tag="IXT21_001329"
     CDS             complement(4325..5515)
                     /locus_tag="IXT21_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4868741.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5536..6063)
                     /locus_tag="IXT21_001330"
     CDS             complement(5536..6063)
                     /locus_tag="IXT21_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4868742.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6110..8899)
                     /locus_tag="IXT21_001331"
     CDS             complement(6110..8899)
                     /locus_tag="IXT21_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ4868743.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9046..9246)
                     /locus_tag="IXT21_001332"
     CDS             complement(9046..9246)
                     /locus_tag="IXT21_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4868744.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9699..10019)
                     /locus_tag="IXT21_001333"
     CDS             complement(9699..10019)
                     /locus_tag="IXT21_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868745.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10522..11640)
                     /locus_tag="IXT21_001334"
     CDS             complement(10522..11640)
                     /locus_tag="IXT21_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868746.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12111..13418
                     /locus_tag="IXT21_001335"
     CDS             12111..13418
                     /locus_tag="IXT21_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ4868747.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13538..14737
                     /locus_tag="IXT21_001336"
     CDS             13538..14737
                     /locus_tag="IXT21_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ4868748.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14764..16032)
                     /locus_tag="IXT21_001337"
     CDS             complement(14764..16032)
                     /locus_tag="IXT21_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ4868749.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16202..16843)
                     /locus_tag="IXT21_001338"
     CDS             complement(16202..16843)
                     /locus_tag="IXT21_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ4868750.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17252..17755)
                     /locus_tag="IXT21_001339"
     CDS             complement(17252..17755)
                     /locus_tag="IXT21_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4868751.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17878..18642)
                     /locus_tag="IXT21_001340"
     CDS             complement(17878..18642)
                     /locus_tag="IXT21_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ4868752.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18750..19025
                     /locus_tag="IXT21_001341"
     CDS             18750..19025
                     /locus_tag="IXT21_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ4868753.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19585..20535
                     /gene="yidC"
                     /locus_tag="IXT21_001342"
     CDS             19585..20535
                     /gene="yidC"
                     /locus_tag="IXT21_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ4868754.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20581..20982)
                     /locus_tag="IXT21_001343"
     CDS             complement(20581..20982)
                     /locus_tag="IXT21_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4868755.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20996..21805)
                     /locus_tag="IXT21_001344"
     CDS             complement(20996..21805)
                     /locus_tag="IXT21_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4868756.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21792..22682)
                     /locus_tag="IXT21_001345"
     CDS             complement(21792..22682)
                     /locus_tag="IXT21_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4868757.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22695..23174)
                     /locus_tag="IXT21_001346"
                     /pseudo
     CDS             complement(22695..23174)
                     /locus_tag="IXT21_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23350..24291)
                     /locus_tag="IXT21_001347"
     CDS             complement(23350..24291)
                     /locus_tag="IXT21_001347"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAQ4868758.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24288..25283)
                     /gene="lacD"
                     /locus_tag="IXT21_001348"
     CDS             complement(24288..25283)
                     /gene="lacD"
                     /locus_tag="IXT21_001348"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ4868759.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25276..26472)
                     /locus_tag="IXT21_001349"
     CDS             complement(25276..26472)
                     /locus_tag="IXT21_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4868760.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26490..27218)
                     /locus_tag="IXT21_001350"
     CDS             complement(26490..27218)
                     /locus_tag="IXT21_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4868761.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27659..28633)
                     /gene="lacD"
                     /locus_tag="IXT21_001351"
     CDS             complement(27659..28633)
                     /gene="lacD"
                     /locus_tag="IXT21_001351"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ4868762.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28630..29088)
                     /locus_tag="IXT21_001352"
     CDS             complement(28630..29088)
                     /locus_tag="IXT21_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4868763.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29085..30548)
                     /locus_tag="IXT21_001353"
     CDS             complement(29085..30548)
                     /locus_tag="IXT21_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4868764.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30530..31462)
                     /gene="pfkB"
                     /locus_tag="IXT21_001354"
     CDS             complement(30530..31462)
                     /gene="pfkB"
                     /locus_tag="IXT21_001354"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ4868765.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31604..32272
                     /locus_tag="IXT21_001355"
     CDS             31604..32272
                     /locus_tag="IXT21_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4868766.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFKSIICQIKRDKTFHLRNSKKLLMVFGN
                     LVIF"
     gene            complement(32196..>32377)
                     /locus_tag="IXT21_001356"
     CDS             complement(32196..>32377)
                     /locus_tag="IXT21_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4868767.1"
                     /translation="SNGLLRKDGLPKEMEFNQVNQGFISSVASKRNHIPRKSLNYQTP
                     LEVFLSYVNGKFCLA"
BASE COUNT         9439 a         6948 c         5552 g        10438 t
ORIGIN      
        1 aatttcttcc atacagatgt tttggcttat tctttctttt tctcttcgat cattgcccct
       61 gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact aagtttgaag
      121 cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata tcttttttca
      181 atccctgcaa aatgtccttc ccagatattc gaagctccac tacgctttca aaaatataga
      241 aagagttctt taatgaaaat tatttcaact ttccataaac agtaatccca tataaataaa
      301 agacgtaaaa gtcacatggg agaacttata caggaaaaca catagagtca ggtgaaatac
      361 atgtatccca cctgactcta tgcgttttct aacctttatt attctctttt tatacaattt
      421 ctgcaccgaa tggcatcaac gccaatcgag caagtttgaa agattgcttg gcaaaaggta
      481 tccctatgat cgtgatacat agaaggatgc cgctgacgac atgtgcgaga gcaattggta
      541 ggcctgaaac gatcagccag atgatattaa cgatcaatga catgccagac tggtgataaa
      601 ttacttcttt gccaaaaggc gcaaaagaaa gggaggcgat cttaaaacat tgcataccga
      661 ttgggatacc tatgatcgtg atacaccaaa gtatccccgc tgctaaccag ctaagaccac
      721 cgattagtcc gccgaatata aaccagatga catttcctaa acaactcatt gtatttctcc
      781 ttctattact gtttttgatt aaaactgggc aaaaattgat tttattcttc tttttttagc
      841 ttccagcatc aagtattctg caaatttttt ttggatttct tccgtatttt cttttgttac
      901 taatgtgtgt tggattagtt ttcctgatgg tcgctgatcc acccgttctt ctgactgttg
      961 cacgtccgct tgcgcaaatc cgataatccg tttttctttt gttacagcaa cgagcaatag
     1021 tttattctta tcatttaggt cagctgccgt ttttgctttt acgaatgatt ctggatgatc
     1081 agtaatcagc tgattgattt gaggtaaatc ttcttttact gcccaccgta tttccatcat
     1141 ttctcacttt tctttcgctc atttttatct ccactaaaaa aatcgactgc ggcaattgca
     1201 tctatccagc tttgtgcaat gatctcatgt ccgccaatcg ttgggtggac cccatcttct
     1261 cttgtatatt tcgaaaaacc ataagcaatc cctaaagcat tgaatatgcc atctaaagga
     1321 accagcaatg cgccatattc cttagcaagt ttacgaacaa tttggatttt agggtctaag
     1381 tctttacgcc agccatgacg gtcgtcggtt ataggaagta caaacggctc catcaagatg
     1441 atgttgcaga tttccgctgt ttgaagttca tttagtaatt ttcgataaac ggattcaaat
     1501 tgctgcgcag atttctcagt tccgaatata gcctgatcgc tagtattatg ccaggtatca
     1561 ttgattccga tcaaaatcga tacatagtca ggttgtaaag ctaaacaatc ttcttgccag
     1621 cgtacttgca gatctgcaat tttgtctcct cctatcccac ggttcatgca ttcaaatttc
     1681 atcgttggat attgggagat tagttttccg gcaatcaaaa gtggataacc tttacctaga
     1741 tcagttgctt ctgctcggtc tcttcctgcg tctgtaatgc tgtcaccgat aaataatata
     1801 cgttttttca tttgtcaaaa actccttttt gaataaaacg atgattttct tccttatatt
     1861 atcccataag ccctatcaag acgaaagaga aaatatatgt ataaaaaatg acaccagcct
     1921 agtcccttca atcaattgaa tgaacgctca tcaagtgtct agttttttat actttttttc
     1981 ttaacgtgcc ggattcccct tgattccttc gttgtttggt ccagagaaat cgatcacacc
     2041 taagcttccg tcaatcagac cactgatgac gccatcagaa catatattta taggctcatc
     2101 taagacttcc agtaaatgtt cttttactaa tatctcttct agattgcttg ggatagattg
     2161 gtcataactt tatctggtcc gatgatttca aggatgaatt aaacttacat atggatcatt
     2221 gcatgctgaa aatacggacc tactccatct tcgaatgtta ctattagttt gttttcaggt
     2281 actagatgct gctttatttc aacagcagaa gcatcatcaa atcagatttc catttgaata
     2341 accttctttg tatatgtttg attgattgcg atttcgttat agaaccttgt gaatcaaata
     2401 gcaatttctt ttgctttctt ctcttgtctg ctggaaactt cctttatccc gtattttaaa
     2461 aaaagaactg cgagagttcg cagttctttt ttttaaataa acgttgctct ccggccaact
     2521 cctcggtcac aagtcacaat agtcatcaaa catgaaaaac tgttttttga aattactcct
     2581 tgtgactcgg agctgaacgg caggcgcaca acctcttgat ataaacggtg ttcaatcagc
     2641 tgaccctcga tattagctaa aaaaacggta aaatatgaga agctattttt ccgttttgtt
     2701 tctctaatac tcaggtcaag gcactgattt cacaacctct tccttaaacg gtgttcaaaa
     2761 gccagtttct cggtattaag ccaaatttat gcaaaaatgt ggaaagccat ttccacaaaa
     2821 tttttcttaa tactcgaaac taaacagctt tttcacaacc tctccactaa acggtattca
     2881 aaagcttaca tctgcggtaa taagtcccgc caattcaaaa acatgaacag ctgtttttgc
     2941 ttggctgttc taattacttg atgcagaaca gctttttcat cacctcttat ggataaattc
     3001 ggttcaacaa acgtgggaat ggaatagctt cacgtacatg gtcgatacca gcaatccaag
     3061 tgactgttcg ttcaaggcct agaccaaaac cagagtgcgg aacagaaccg tatttacgta
     3121 gatcaagata ccatgaatat tctttttcat ccaaaccgtg attacggatt tgttctagca
     3181 agaaatcata gtctgttgcc cgttcgcttc cgccaataat ttcaccgtaa ccttctggtg
     3241 cgatcatatc tgcacaaatt accacgtctt ctcttgttgg atgtggtttc atgtagaatg
     3301 gcttgatggc ttttggatag tttaggataa agacaggctg ttcgaatgaa ttcgcaatga
     3361 acgtttcatg aggtgaacca aagtcttcgc cccattcgat gtcatcaaat ccatttttct
     3421 tcagtaattc gattgcatca tcgtaagtaa tacgagggaa tggcaatttt gtataagttt
     3481 ttaagacttc tttgtctcgt cctagtacat ctaatgcata ttcgcaatga tctaaaacat
     3541 tttgtactaa gaatgctaca tattgttcct gtacttctaa actttcttct tgatgagtga
     3601 aagccatttc tggttcgatc atccagaatt cgatcaaatg acgtcgtgtt tttgattttt
     3661 cagcacggaa agtcggaccg aatgaaaaga cttttccgaa agccatagca gcagcttcca
     3721 tgtataattg gccactttga gataaataag ctttttgatc aaagtaattt gtttcgaata
     3781 gatccgtagt gccttctggt gcagaaccag tcaagatcgg cggatcgatt ttgacaaaat
     3841 gatgttgatt aaagaattca tacgttgcac gaatgatttc atttctgatc tgcataatcg
     3901 catgttgtct tgaagaacgc agccataaat gacgatgatc catcaagaaa tctgttccgt
     3961 gttcttttgg tgtgatcgga taatcgtggc tctcgcttac cacttccaca tttgtaattc
     4021 cgatttcata accaaatttt gaacgactgt cttcacggat ttctccagtt actaacacgg
     4081 atgtttcttg gtgtagattt ttagctgttt ggaaaacttc ttccgacact tcgcttttta
     4141 caactacacc ttggaaaaag gctgttccgt ctcttaattg taaaaatgcg atttttccgc
     4201 ttgatcgctt atttgcgacc caagcgccga ttgttactgt ttctcccaca tggtttttcg
     4261 aatcgatgat ttgaatttgt tccacgtatg actctccttt tatattcctt gaatttcttc
     4321 aggattaatt ttgactttta ctctcaataa attgcttgat tctgcgtacc gcttcttcta
     4381 acgtagcgcg atcagtggca taactcatgc ggacattttc tggtgcccca aagccttcgc
     4441 cagtcacaag cgctacccct gtttcttcta aaagatcttc tacccattgt gtgacatttt
     4501 catacttaca catgcgtagc gtttctttta cattaggaaa aagatagaaa gctccttgcg
     4561 gcttttcaag agaaacaccc ggcagttctg ccacaagcgg atataacgta ttcaggcgtt
     4621 cttcaaaggc gttccgcatt tcttctactg tatcttgctc acctttcaat gcttcaacag
     4681 ctgcatattg actgactgct gtcggattgc tagttgattg ggaagcaaca gcgatcattc
     4741 cattgatgat cacaggattt cccaccgcat aaccaatgcg ccagcctgtc atcgcatatg
     4801 ttttagacac gccgttgatg atgatggtct gatttttgat tgcttctgaa atcgttgcaa
     4861 ttggagtaaa ctcattgcca ttataaacta aacggccata aatatcatca gcaactatca
     4921 aaatatcatt ttttactgcc cattcaccga ttgcctgcag ttcattcttg ctataaatca
     4981 ttccagtcgg attcgatgga gaattgatga ttacggcttt tgttttttct gaacgtgctt
     5041 gttctagctg ttcaacagta actttccagc tattggattc ttcccctttc acaaagacag
     5101 gaagtccttc tgccagtttt acttgctcac cgtaactgac ccagtaaggc acaggaatga
     5161 tgacttcatc ttgcgggtca aggatcgttt ggaaaagggt atacaaagca aatttcgctc
     5221 cgtctgttac gactgtttgt gaaggttcat agacaagtcc atagtttttc ttcagataat
     5281 caacgattgc ctgtcgaagc tctgggattc ctgctgttgg tgtataatag ctagctttcc
     5341 cattgcggat tgcttcgatt gctgcttctt ggatgttttc tggtgtcgca aaatccggtt
     5401 ctcccacagt caaactcagc acatctctgc cttttgcttt taatgccttg gcctttgccg
     5461 cagctgccaa agtgacagac ggttctaatc gttttgttct ttttgataac tccatcccat
     5521 tcattccttt accttttaaa ttcccttgat tgtcttgact tcatctccag attcaaagcc
     5581 aatcaggtaa taattcagtt tcccatttgt ttcttttcct actacttccc acacaggctg
     5641 atcatcatac atccctaaag cagctttttc gatttggatt tcaggatggg ccgcagcaat
     5701 tgtctgtttt gcttcgtttt cagtcagtcc ctttgtttga tccaatactt ttactttttc
     5761 accagattta ggaatgataa cggcgatttc ttttccttta tcatttgtac ctgtcagtga
     5821 aaagtatgtc ttctttcgag taaaccaata aaaatgatcg gtttcctcaa gatttgcata
     5881 tttctttgcg atttctactg cttctttctt cgcctgcacc gccggacgac tggaacgcaa
     5941 gtaaaaaatc gagctgatca cgatgaccgc taataaaaat acgatcaagc caagtaacac
     6001 gcgattaatc gttttttcct ttgtctgttt tttcttcaag ggtcgtcgct cctttatctt
     6061 cattcctttt cattatagca gattttaatt atgaaaaaag acagctcctc taacttcctt
     6121 cttcattttt ctttaagaat tcatggatat cctgatagat ttcctccata gctagctctt
     6181 tgatcggtac ttttttcggt aatgcatgga tgatccgtgc gccatatttt gcagtgacta
     6241 gtcttcgatc caaaataagc aatacgccac gatctgtctc cgtccttatc aagcgaccta
     6301 atgcttggcg taacttcaaa gctgctttag gtacggattc ctggtaaaaa ggattgatcc
     6361 cctctgattc taaataagcg ttcctagctt tgaccatcgg gcgaagcgga ttatcgaagg
     6421 gtaaacgtgt gactacaacg atctgcaaag catctccagg caaatctaca ccttcccaaa
     6481 agctgtcagc gccaaacaat acgctttttt ctgacaaaat gaagcgtttc aaaagcttct
     6541 cccggcttcc accaaaccct tgagctagta tttctcgtcc tttttctagc atagacagat
     6601 gcattttctg atagaccttc tgcaaaacat catgagaagt gaataacaca agtattggcc
     6661 gatcttcttg ttcgatcaga cctgtaagtg ctgtctctaa ataatcgata tatgcttgat
     6721 tgctgacttg atggacggca ggtgcctctt ttggcacata gatccttgcc tgtttctcat
     6781 aatcataagg agaggcaatc actttaagag gtgctgcttc aatgccaagc ctcttagaga
     6841 gatagtgtcg attagggccg atcttcagcg ttcctccaat ataaagaata cgatcatatc
     6901 gaggatacca aacagtcgac ggcaacatag ccgcttgaaa atcatggaga tgaaaaatca
     6961 tctgctgcgc tgtgttccca ggaacgaacc aatggatcaa atgatctcgc cagtcttcca
     7021 gccattgatc catcagcaag tattgcttgt ctaattgatc aaataggcta tataaacttg
     7081 ctaaatcgat ccgcatctgg tttgtccagc tttcttggat aatctcaaaa gtcagacgca
     7141 tttgtgactg tagctgctgg atctcctgat aaaaaagctg caggtgatca atggctgtcc
     7201 cagattccag tggcagctga tccaacatct cttttgtcat catgacttct tctggatact
     7261 gtccgttagg ttgcttgccg ctcactaaac tcattaatcc ttctgccata caaatctgtg
     7321 cttcgcatag cgcagaaagt tcttctcgat aaatcgataa ccagcgtaag gcttcatgtt
     7381 ctttcggtaa atagctttgt attcggtcaa acagctgatt tggttcttct aattgctgga
     7441 tttgtttatg gaaatagtta atacttaatc gtttgtcatt tactttttca gcgatttccg
     7501 gcaaatgatg tacttcatcg atcaacaaaa atggactagc tggaagcaat ggttcttttc
     7561 gctgtgtttc ttgtactaaa aacgcatgat tgacaatcaa aaaattactt tgcctgattt
     7621 tcttttgcaa gaaacgaaca aagtcttgtt cgtaaaatga ctgatttctt gctaaaaatc
     7681 caatacctct gtgcgcgata tctgcaaaga atgaatggtt caaatttgtt aaatgtaatt
     7741 catccaaatc accagtcttc gtttgcgtca gccaaactaa gaccgccatt tgatacaaag
     7801 catattgttt ttgttctggc ggttccacaa gtgtgccttt aaaacgctgc aaatcaatat
     7861 agtggcgata gcttttgata attacaggtt gtatgggctg atctagcaat tgattcagca
     7921 atggaatgtc tttttctatg atctgctgct gcaaaactag cgaaacggta ctgatgacag
     7981 cgggtttttc aggtgtagcc aaataactca atggcagcag ataaccgatt gttttaccca
     8041 ttcctgtagc tgcttcgatc atcaaatctt ttgattcatc ttttgtgaaa tggtcgtaga
     8101 ccgcattcat cagtctgttc tgttcttttc gatacatcag tgttttacca aacatttttt
     8161 cttttgcttg tttttttctt ggatatgtcc gctcgccata atggacggat gtgaatagtt
     8221 ccacttcttt tttctgcaaa gctagcccat cgacgatttc taatgaggga tcaagtggct
     8281 ctggtttttc tttcatttct tcaatcacat gataaatgaa acgactcgta tccatacctg
     8341 tgtgtctact taatcgcgca atctgctcta acgtgatgat tggcaatttt ctcatccgtt
     8401 cttcgatcag caaaagaagt tgacctgtca cttcggcatc actatctgcc tgatgaggat
     8461 tctcatgaat aaatccaagg ctttctgaca aatcagctaa gcgaaagctt ggttccgtcg
     8521 gcaagaaaat ttgtgcaagt tctactgtat cgatgcctgg tatcttcaaa ctaggaagtc
     8581 cgcatcgcat gagttcatgg ttcaaaaaat tataatcaaa ataaatatta tgcgcgacaa
     8641 atatggtgtc tgccaaaaga ttgtaaatcg tttgggcgac atcttcgaaa taaggcgctt
     8701 tttggacgcg ttgattcgtg atccctgtta aatgctggat ttgttttgaa atcttccgcc
     8761 ctggatgaat atcgatcgaa aaacgtgaag tgatccgacc tgattcaatc atgacgcagc
     8821 caaattggat gatccgatct tcttttggat tcgtcccagt cgtttcgata tcaacgactg
     8881 cataagttct tggtttcaaa atatcttcct cttctcctgc ctctaatgtt cttttgaaat
     8941 tatactacaa agtgcagaaa aatactctca tcgcttttta aagaaaatag acaaaaacgc
     9001 ccagacacgc aaatgtctgg gcgtttattt tttagtatag gttaattaag ctttagtaac
     9061 gtttgttgct tgaggtccgc gttggccttc ttcaacatcg aaagttactg cttggccttc
     9121 ttctaaagtt ttgaatccgt cgccttggat agctgagaaa tgaacgaata catcgtttcc
     9181 gttttctgca gtgatgaatc caaatccttt ttctgagtta aaccatttta ctgtacctgt
     9241 ttccataata aaaatcctcc ttgtgtatca acacatattt tgcaattact tgaatggtgt
     9301 atatctggaa gatgtttatt tgaaacaagc taccacagat taccgaacaa aaactactcc
     9361 tatagtatat cataccgttt acaaaaaagc tagagttttt cgactttttt tattaaaaaa
     9421 agttttctca gcgattacat aaagtgaaaa accgaacttt tttttaaaat aaacgacata
     9481 ttgttcagtt ggtgtttttt tgctctgatg ttctttttta aactaaaaaa attattcttt
     9541 ctgactactt atcggttctg tttcaacggt cgaggctggg gaagattctt tagtaggtct
     9601 gatgttctca tggtatttct gatagcctat gacagttacc agaagaacaa ggcaaaccaa
     9661 tacgaccaac gagattagtt tttctttttt gctcatatct atacctcata aataacagga
     9721 tgatgtccat catatttctg agtatgagta aaagcaaaag ctgcagctgt agaatgtaag
     9781 aaatgccagc aactttcata atcacaggtc ggacagtacc aaaagtaacc aaatcttggc
     9841 tgcaaagacc gttcttctgt ttcaggaatc gcagaatcaa gatctggtgt ctgtacataa
     9901 acatttttgt acagacgtgt gcctgtaata ggaaaccgct gtgtttcttc agcaaaaact
     9961 ggctgacttg ttaaacttag ggatagagac agcaaacacc ataacagtct ttttttcata
    10021 aagacctcct tatcgataag tttttcccaa ttaaaatcat atctcaagaa cgcccgttat
    10081 tcacattttg ttcataaatg atgttttttg tatcataatc gtcaaaaaga ggtcgtgacc
    10141 acgtcacaac ctctttttta aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
    10201 atttcacaaa aatttgagaa ataatttttg tcaatctctt cttatttctt gaagctgacc
    10261 acttctgtca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc
    10321 ccaccaaaca aaaaatatga agagctattt ttgcttgctg ttcttattac ttgatgcaga
    10381 acagcttttt cacaacctct catacacggt gtttaatcag ctgaccttcg gtattagctc
    10441 aaaaaacggc aaaatatgtg aaactatttt tccgtttgtt tctctaatac tcaggtcaaa
    10501 acactgattt cacaacctct tttaataacg aagaatcgct gtcagactct tttcatctgg
    10561 cgcatctttt tgatccaaaa taaacacttt tccgccatta atgatcacat catctgctaa
    10621 tgcattcaac acttgacgcc gatcatattc tgtttcagga tcttcaccga aaccatcagc
    10681 taagtttgac gtagcgataa ataaatgtga cacttttcct tctttcgcag ctggaacgat
    10741 atcaactaac tgatcgatga atttcttatc taagagttta ttgtattctt ttgattcttg
    10801 agctgcgagt tcacttgaca gcttcttcgc cccttcttcg atatctctaa aggatagctg
    10861 cgcaggagaa gcagaaatcg caacgtctgc acaataatag ggatttttag caattttttt
    10921 gaacatcgtc tgattttctg gtagagcaaa taaataaatc ggccattgtt ctggattttc
    10981 gaaagaatcc ttcaagtacg tatcaactgc ttggtaatag ttgacccaat cgatctctac
    11041 ttcttcatct tttggattta cgccatggaa agttactcca tctcgggctc cgcctcctcc
    11101 attagaagag aagttcaaac cgccacctgt cagttcagat cccaatgcag taacgacatc
    11161 aaccggtgcc ccttctggta gagatacttc ttttacttgt ttgttgctga cttcatacaa
    11221 tttcatggag tcgcggttca atcccaataa ataataagaa tagttgaact gcccattttt
    11281 gataatagcc agtaaataag gaagatcact tacatagtat tgatcgtcta ccgggatact
    11341 caaacgatgg atataggttt ggtcagcagt tataatgacc gctacactcg tagttgcact
    11401 acgccaaaac gaaggatctc ctaacaaatt ttcaattttt tcttggaacg gggaccagtc
    11461 atgttcggaa tattttttct caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt
    11521 gatctgatct ttttccacat cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa
    11581 tggtccttta gcgccttctg aagtcaattg tgacaataat tctttatcct tttttgccat
    11641 aaacgtaccc ctcctaaaaa aatcattcct ttgtttctat ctaaagtcta ccacaacact
    11701 atttttccat acaataaaac gtatctaaaa aaataaaacg gtttctacgg ttttaaattt
    11761 agctattttt ttaagttatc ttcataattt cctcaattat ttagccccag tcttcaacta
    11821 ttcttcatga aatgctgata aactaagaaa aagaataaaa gaggtggatc ttatgcattt
    11881 gattatgatt ttcagtttat taagtattat cagtttattt gctttcagcg cttcccgctt
    11941 attagatgcg gaaacacagc ctattcctgt aaaagtacgt aaaaacgacc gctaaccttc
    12001 cataacgtgc gttcttatcc taaagttttt tcagaaacat tgaaactctc tcccctctct
    12061 gataaaattt taatttgtag cgaaacaaat taattacaag gagatcggat atgtccataa
    12121 aaaaaacacg tctagtccta ttattggcta gtctcattct ttgccttgct tcttttctac
    12181 cagctgttgc tgtgcaagct gaagatactt tcaaagtaaa tgcaaaagca gcctttgctg
    12241 tagatgcgca aagcggtaag attctttatg atcaagatgg agaaaaaacc atggggatcg
    12301 cttcaatcac aaaaatcatc ggtctttaca tcgtccttga tcaagtaaaa gaagggaaac
    12361 tatcatggga tgataaagtt tcaatttcag attatgcaga aaatttgagt gtcaccccag
    12421 atttatcaaa tgttcctttg cataaagaaa acacctacac cgttaaagaa ttattcgact
    12481 ctgcaatcat ccagtcagcc aacgcttcaa tggttgcctt ggcagaaaaa atctctggca
    12541 gcgaagcaaa attcactgaa cgaatgaaag aacaattgaa agattggggt atcaaagatg
    12601 caacgatcgt taatgcatca ggactgaata actcttatct tggcgaaaac cgaccagaag
    12661 gtactggaga aaacgacgaa aatcaaatgt ctgctcaaga tgtggctatc gttgcccgtc
    12721 atttgatctt ggattttcca gaaatactgg atgtatctag cactacgaca caaatgtttg
    12781 gcgaaaacac gcaatcacca gtagaaatgg tcaactggaa ctggatgctc ccaggattcg
    12841 tgaattataa agaaggcgtg gacggattaa aaactggtac gactgaactt gcaggagcct
    12901 gtttcgtcgg tacgatcact aaaaacgatc aacgaatcat cactgtcgta ttgaatgcag
    12961 acggccatgc ggaaaaccca agtgcccgtt tcaatgaaac agctcgctta atggattatt
    13021 gctacgacaa ttggtcagaa aaagagcttg gaaaagccaa tgcaagtatc cctagtctaa
    13081 aaaccattga tgtaaaagac ggaaaagaat caacagtgcc cgttgtgctt aaaagtcctg
    13141 taaaagtatg ggtacggaac gatatggata ctggaaaatt aacaatcaca ccaactcttg
    13201 acaaaaaaca agtcactgac aatgagatcc aagcacctat ggataaagga acaaaaatcg
    13261 gtaccgcaac gatcaccttg gcagaagata aattaggtta tcttgaagac aacaacgcgc
    13321 catccacaga aatcatcacg gataaaaccg tagaaaaagc aaatattttc gttattggct
    13381 ggcgacgcgt cgcagaattt ttcggtaact tgttttaatt gaacaaaaag aaaaaagcat
    13441 ctttccatgt ggaagatgtt ttttgctttt tctagtttaa ataacagctt ttttcatcta
    13501 ttttcgttat actgtataag aaaaaggagt tgaatttatg aatctaacca gagatctgat
    13561 ttttttggtt ctctatctta ttggggtatt ctccatcgtt tattggcgaa aacagaaaaa
    13621 ggatccttcc ttgttctttt acagcagtac atggacgttt gcggtaatct tgacttattt
    13681 tgtcgtgttg gaaatttttt attcgacctt ttattttttg attcccttag tgtttttcag
    13741 tatctttgcc ttttcctatt tttcagaaaa gcgaaggctg ttgaatggtc ttttattcaa
    13801 tattttcctc attagttttg gtatctactt gtttgtcttg ctatacgaaa cacaaaatat
    13861 ttttcttggc ggactgattg ccttgatcac gatcccgtta ctgcttgtct ttctattcgg
    13921 catttatgga ttgatcgttt ttcttttttg gaacggtgtc acagttttac gtagagagtc
    13981 gcattcatta gctaatcttt taacgttgat actagccatt tttcttacat tgtttcttgt
    14041 ttttgacttt ttcttgttaa aatatttacc acaatggata aatgctttgt ttttctgcat
    14101 cccattgatc ttgatctatc tatttatcgt attctacaac tttttgactg tctcattctt
    14161 gtatcagttc aaccgccctc gatacaacca agacttcatc gttgtgttag gcgccgggtt
    14221 gatcaatgga gaaacagttt ctcctcttct tgccaagcga atcaataagg cgattgcttt
    14281 ttattgggca caaagccgtg cgacattaaa tccgcctatc ctattaatgt cgggtggaca
    14341 gggagcagac gaaaaagtac cagaagcaat ggcgatgaaa caatatgcca tggaacaagg
    14401 aattcctgaa agagatattc ttgtcgaaac caattcgaca accactttgg agaatatgcg
    14461 ctattcaaag gaaatcatgg atcagcaaat gaaaggtccc tatcgcgcga tcttcagttc
    14521 caacaattac cacattttca gagctggtct ctatgcacga caagcgaaac tgaaggcaaa
    14581 tggcattggg gcaaaaacag ctttctatta cttgcctaac gcgtttttaa gagaatacat
    14641 cgccatcttg atgatgaaca aaaaaagaca tatgatcatt tgcggattgc ttttcgcagg
    14701 ttccgcattc ttatcactga tcactttact tttctaaaaa aacaaggtgt gacaaaacat
    14761 atgtcacacc ttgttttttt cttttattct cttgacgatt ggcaagaata gtattccttt
    14821 tttgacggct acttgctgtg cagaataaat accgctgttt cctgaacaca taaagctgat
    14881 caagcagatc ataaagaagt aagcagcagc ttgtgaacca aataattcga ttcccatcac
    14941 aaaacaggca atcggtgtat ttgtcgcacc agaaaatacc ccgataaaac ctaatccagc
    15001 aagaaaagga acagaaacat ggagcaaagg cgctagtact gcgccaagcg ttgcaccaat
    15061 ctcaaatagt ggtgtcactt ctcccccttg gtaccccgca cctaaagaca agaccgtgaa
    15121 gaataatttc ccaataaaat cataagcatg tgcctgtcca ttaaatgatt cctgcagtaa
    15181 cggaagactc aacccaaggt aacgttgtgt ttgcaaaatc aacgctgcgg ctacaactac
    15241 tgctccacct atcgtatttc gccaaacagg atgaggaatc cattgcgcat accgcgcttt
    15301 taaaaagacg atcgaacggc tgaataccca accaatcaaa ccaaaagcaa tcccagcaac
    15361 aaaaactttg aagaacagca acatactcca ttcaggaatc acacccattt ggtaatgtag
    15421 atgagaaaca ccatagcttt ctgtcatgaa atttgcaaaa agcgcagcaa agaagctagg
    15481 aaatattgct tctgttcgga ctttaccgat tgccaacact tctaatccaa ataatgttcc
    15541 agctaaaggt gtgccaaata cagaactaaa acccgcactg attccactga taaccaaaat
    15601 ctcccgttct gctttatcca atcggaataa atgcgctata ttatcagcta atgctccccc
    15661 catttgaact gctgttcctt cacgaccaac agatccccca aataaatggg tagtgatggt
    15721 cccaaacaag gtcaatggaa tgagccgcaa agggatcttc tcttctcctc cattcccttg
    15781 atcgatcact aagttattcc cacgactaga aagaccgcca tgatacgcat aaagataagc
    15841 aaaaagcgcg ccgctgactg gcaatagata aagtagccaa ggatgtgcta gtcggatatc
    15901 tgtcacgaac gtcaggctat ttaagaaaaa agcagacaag ctccccatga atactccgag
    15961 aatcgctgtg accaccagcc acttcaacat gtacatcaaa atacgcactg gactcggtaa
    16021 ttgtttttcc ataaaaaatt cctcctcaaa taaaaaaacg cctactaaag aaaggggctg
    16081 gccccagact ttagtaggcg tcattagcta tcttacgtta gcatttaaag gcgaacacca
    16141 tcacctattc acttccatta aaagatagca tatttttctg taaaaaacaa taaaattatc
    16201 gttatttctt tacacgttca gcaaattcat agaaaggagc caatttcgtt aactgttgat
    16261 tacattgatc actatcatga caaatataat tccccttttt cgtatacgtg ccatcagaac
    16321 cggctttcgt tgtagaaaga aagagcgaga cattcgatgt tttctgacag atcgcacaga
    16381 ttcccttatt aatcgttggt gataaagtac cgctgatccc ttttaatttt ccatcttcgt
    16441 aataaaccat gaattttttt tgcgtccctg gatcattcca accgatgtac gtatgtgctt
    16501 gcaagtcgat ttcattccaa gcgggttgtt tgagtttttt cacttttcgg aataatttct
    16561 cgatttgtgg tttgctcggc tgcataaacg gctggacgat ttgcttcagc tcctccaatg
    16621 ctcgttctgc ttgcgttttt gtcagtttta catctaaaac tgtcgttaaa aacgtatcga
    16681 tttctgatgg ttcgtaacct aatacatcgc gaatcttttc agctgctaga gcctgaaccg
    16741 tttgtaccgt ttttgagtca ttgacagatg agtacgcatt taataactgc tctacttctc
    16801 tttttataaa aaaatattga tatggtttca ttgtttgttc catgatttta aagtcctttc
    16861 ttctaaccaa agtgcatact ttgtagaaaa aagtgatctt ctgcaaagta tgtgttacct
    16921 gaagatcccg gactctttta aataagatac gttttgcatc ttatcatcac cccctttaat
    16981 gttcattctt ctatgtacat gattcattcg ataaataaaa gtaaaccgta tcttgagcag
    17041 atagtcaact gcaaacagaa aaaggactgt gacaaaagtc gtgtcaaagt ccctttctac
    17101 aaacaaacgg tgttccagaa gtagcttctt caaaaaataa gccggaattt cacaaaaatt
    17161 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgacccact tctgtcacaa
    17221 cctcaatatg gtctattatt ggttttacct gttaaatcga ggattctttt ccgaagtaac
    17281 gttgccatct cttacgcata cttgctgaca taggctgagc ggcttctttg actgcgcagt
    17341 atttgtctac gaatgttacg atatatcctt ctttgtactt aggcggtgca atcgttgcgc
    17401 cccacatgtg cttcaaaatg atatctcgct caagatccga caaatcggtg atcttttctg
    17461 cattctttac agcgattctt ggatggatat acgcatgtgt gccttcatca aatttcgtcg
    17521 tacgccaatc atagtagaac agatcatgca aaagtccagc acgcgctgtt gctttagcat
    17581 tgccacccca tttttttgcg agcagataac tatagtaaga aacactgatg gaatgctcca
    17641 aacgagtgga atgaacatgc tgtgtgtaat tcgctaattt tttcactgct tctgtttcta
    17701 aaagatcttc tacataagac atatattctt cgtcttcacg ccatttttct gtcataatta
    17761 tcgatctgcc acctttttga atttcatttt cttctgaaat tgatctaagt ataccaccag
    17821 tgttcggatt attctagtcg ccttggataa aatagccaaa gcttaatagt tccttaatca
    17881 taaagaaaaa tgataaagaa caatccctgc cgcaacccca acattcaacg attccgcatt
    17941 tcctttcatc gggatatata cgttctgatc agtcaaagac aagatttcct gacttacccc
    18001 ctgaccttcg ttccccatga ttattgcgaa gttatctgat ttttctactt tgtctaaggc
    18061 gtgtgcttct tcatttaatt ctgtgccata cactgtaaac cctttgtctt tcattaaagg
    18121 aatcacttct gtcaaatctt tttgcaacac aggcaaatga taattgcttc cctgcatgct
    18181 gcgcagcacc tttgtactat aaatatctgc cgttcctttg cctaataaaa caccagccat
    18241 atcaaatgca tctgccgtac ggatcatcgt ccctacattt ccaggatctt gtacattatc
    18301 taataacagc catcctcctg aaaaatctgg cgtgccttca gccgttaacg ccatcactgc
    18361 taagattcct tgtggtgtag gtagatccga tacagcatcc atgacctcat ccgaaactag
    18421 aaaagtagaa atctctttgt ttgcggccag ccagtctccc cattctttca tgcctcgctg
    18481 tgtcacaaaa agttctttta tttctgcatc agacgacgca gcttcttcaa tcagatggaa
    18541 cccttccaaa agatattctt ccttttcttc ccgatatttc tttttatgta gtttttttaa
    18601 ttctttgatt cttggatttt ttgctgattg gatctctttc acattcctca cctccccatg
    18661 attatatcat ggaataggtg cttgagcttg tttcatccta aaaaaatcgg tatcattaat
    18721 ttataaaata ggaaagaaag gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac
    18781 gcgtccaagg cgtaggattt cgctacatga ccaagatggt cgctgatcaa ctaggcgtga
    18841 ctggaacggt aaaaaacgaa gatgacggct cagtcacgat cgaagcaatc ggcaatgacg
    18901 acatcatcca gaaatttatt gaagaagtca agaaatctcc gagcccaagt ggacgagtgc
    18961 agtatgtgga tgtccaagaa gacccgatga ttgaggaacg aaagaaattt gatgtggtgg
    19021 gataaggttg tgaaaaagct ggtctgcatc aagcattaag gacaggttgt gaaaaatagc
    19081 tctccatatt tttcaacagg ctggacttaa tgccgtagat gcaagctttt gaacaccgtc
    19141 aataaggttg tgagaaagtc gcttagtttt gagtagtaag gagaattttg tgaaaatggc
    19201 tttccacatt tttgcataaa atttgactta ataccgagaa actgactttt gaacaccgtc
    19261 gataaggttg tttttgagac gctttgcctc aagtattaaa gataggtagt aaaaaagctg
    19321 atctataact tgcaataaat ttgcttaacc catattttcc agacagaaca aaaaagtttt
    19381 tagtttttta aataagaaag aaacacttgg aatcactctt ttctttccaa gtatttcttt
    19441 cttgattttt tgtctttttt tcaaaatttt tcgctacgta aaagaacgaa tatttatatg
    19501 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
    19561 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
    19621 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
    19681 gacacttcgg gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
    19741 ctagttgaga atttcaattg gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc
    19801 cgtatcatca tcttgccact aggtattagt caatcgaaaa aaacaatgat ccaatctgaa
    19861 aaaatgcaag cgttaaaacc acaagttgaa gcagcccaac aaaaattgaa agctgcaaca
    19921 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtataccg cgaaaacgga
    19981 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
    20041 gccttgtact ttacggcacg ttatacagaa ggtattcggg aatcatcttt ctacggcatc
    20101 gatttaggct caccaagtat ggtacttgtt gtcattgcag gggtcgccta tctgcttcaa
    20161 ggatacatct caacgatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgcta
    20221 atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg cgccagccgg tgttacactt
    20281 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
    20341 aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
    20401 gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca
    20461 aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag
    20521 cagaagcgga aataaaaaca taggacaaaa aagatgagag ctaagccctc atcttttttg
    20581 ttactcttcc tcttctgcta ttccattaat taattcgttg acagagacaa tgctttcaca
    20641 tcctgcacga acaaactcgg gctctgaacc aatcatttgt gagttgataa attcaatgac
    20701 catcggaaga ttcatcccag cataaagagt aatctgttgt ccttccatta ttagtttaga
    20761 aatgatattt gcaggtgtac cacctaaaag atcgcatagt accacaaaat catttaacga
    20821 atctgtgaca gtgagaaact tttgtttgta tgtttcagtg ccatcttctg gtaataaagg
    20881 taacgtgtag atatcctctt gaggccccat gatcatttct gtactttttt tcaattcctc
    20941 acaaaatttt ccgtgactaa tcaataccaa ttgtctactc atttttcgct ctcccttatt
    21001 tcgaaatcac acccaatgca gataaagcaa cacacacaat taatacgata aaaatcgctc
    21061 gagtaggagt catgtttttc tttcctaata accaataaac accaccgacg atcccagcag
    21121 gtaaaatttt aggtaagatc atatcgatat agttttgcat attcaatgtc acatcgccaa
    21181 tcgttggtgc ccatgaaaat ttaacattga tcattgtcgc aactaatgcc cctaccatga
    21241 atacccctaa taaagtggct gcgtccgtta atgcagttaa gcgagattgc atagtagtaa
    21301 ctaaggaaat tccttctttg tatgcaaatt caagttgctt ccaacgaaaa accatgacaa
    21361 cgatctgagc aaaaatccat ataaacacac ctatcggatt tccatcagtt gccatattcg
    21421 ccgccaaagc cccaaaaatc gttggaatca aagcagcaaa tacggaatct ccaatcgctg
    21481 caaaggaacc catcaatcca gctttcagcc ccgatacggt ttcctttgaa gcaattccct
    21541 ctttttcttc cattgcaaga tcaatcccag taatgattgt attaaaaaaa ttagaagtat
    21601 taaaaaattg cgtatgtgtt ttcatcatct cttttaattc tggtgtattg tctccatata
    21661 tttttcttag ttgtggcaag atagtatata aatagccaga accctgcatt ctctcatagt
    21721 tccaacctaa ttgaaaagtg aacaaacttc ttcgattgat ttgcttaaaa tcttcttttg
    21781 ttaatttgta attagatttc gtcatcttcg atttccccct caacagaatg tgatggctca
    21841 ctagctaatg gttgatggtc tttgttttga tcattcttat aactgatcat tgctagtgct
    21901 agaccaatca atgctacagc aagcatcggc agcgaattga aagttgccgg aagatcttta
    21961 acaacgcttg ccacacttgt tcctaataat tgaacattag agaataacgt tgctaataat
    22021 gccgtgatcg taaatcctaa aataagataa gatagatgtt tttttactgg taaatagcgc
    22081 agaagaatgg caaacccaac agctggtaaa acagctcctg ctactgaaag tccatcgcct
    22141 agccatttta gattcccgtt taaaacagaa acaacatttt caactaatcc accaccaaat
    22201 gctaaagcta aaaatacagg aatcatacga gagagcgacc aaggtaaagc tcccattaag
    22261 aaattgcgtt cgattgcttg ataattcatg tcttctacgt gacggtcaat acgatgggca
    22321 aagtatgtgt tagcgaatct agctaaaata tccaattgga tcattaagct tgctacaggc
    22381 actgcaatag cagcaatcgc ttgttctgga ttcattccaa gagaaatcga gaaggcagtt
    22441 gctaaaatcg tcccagagtt ggcatctatt tttgatgcgc ctccaaacgt ccccactcct
    22501 aaaactgtca actgcatact gccaccaata atcaaacctg cgtttaaatc acccattact
    22561 aaacctgcat ataaccctgc aaacactggc gcactcattg aagaatagat ctgtaattca
    22621 tctaaaattt gataacctgc atataatgtt aataataaga tctgccacca ttgaatatcc
    22681 atggatcatt cctcctattt gattaaaaga ttcataaagt cttctgcttt atcgtttggt
    22741 accatttgag caattaaatg tataccaagc tgatctaatt ttttaaatac ttcgatatct
    22801 gcatctacca cattgattga ttttgtaatg gaccgtgttt cattcgtttg tgacatattc
    22861 cccacattta cttctttgat aggaactcct ttttctacta aagataagat tcgatctggc
    22921 ttttttgcta cgatcaatac tcgttgagag tcatatttgc cagctaaaat atttgttgct
    22981 gctttttcga ttggcaaaat gcttaatttt acccctgctg gagtcgctaa cttcaatcca
    23041 cttttttcaa ttgtgtttgt ggctacctca tcatccacga ccatgattcg actaatattc
    23101 agtttcgttg tccaaagatt tgctacttat ccatgtatca aacgtccatc gattcttaca
    23161 ccaataattg ccatcatgca ttctccttat ctattttttc tatttattgt tcaattaaaa
    23221 ctgtgacatc atcaaatatc ccttctgttt caaagataaa gttttcattt tttctttctt
    23281 atatttcatt tttaagctaa gaacaaattc cttcccaact aaagaaaacg ctttctttta
    23341 tatttaagtt catttgattt tcttaacttg aatgtgtttc atcaacatcg taactttcat
    23401 agggtcgatc cagccagttt tctcttgttg cgcgtttgcc ataccacaag ccatagaaaa
    23461 tgcgagaatc tcttcgattg attgcctttt tgataacgca tgggccgatc ctgcaagggt
    23521 cgcgtcacct gaaccaacag gattaacaac tgatatttta ggaataatga cttcgtaaaa
    23581 acactgagca acttttgcca cagcacctct ctctcctaaa gaaacaacaa cccatggcac
    23641 tccttcaaat attggatgag ctaataagga ctgaattttt tctaattgaa tcgaagaaca
    23701 gatacgttgg ccaatcagtt ctcctaattc ttccagattg ggcttgataa gtagtggctt
    23761 ctccttacct tccaatactt caagcaaaaa tttaccagaa gtatctagta atatgtcttt
    23821 ccctttttta tcagctaaag caaccaattg cttatagtaa tcaagagggc atccttgggg
    23881 taaactacct gagatcgtca taatatctga tatttcgagg agctgctcga aatgatcggt
    23941 aaatgtctgt tgttctttct ttgttatgaa ggggcctcgt tccaaaagct ctgtttgtct
    24001 cgaactatgc aggatagcaa tagaatccct tgtctctcct ttaattggaa caaactgatg
    24061 catgatccct aactgatcta gctggttctt tagatattca ccgtgctggc cacctattaa
    24121 accagtagct atcacctctt ctcctaataa atttagaacc ctcgccacat tcactccttt
    24181 tcctcccgct gttttattga ctgtttcaac tcgattgact ttgtcgaggt ttaattcttc
    24241 aattgtatac gcgatatcga tggaaggatt caaggtaact gtcgtaatca tttcatcttc
    24301 acctttttgc tttttgtttt attgaaccaa ggggtagcgg tttcatttat tactttattt
    24361 aaagtctcga tgttttctct cccatttgtt cttaaccaat gaatagctcc gtcttttcca
    24421 ttttcgacgt atgctttgac cccatctttc catgtcgcac gtccacacaa tacaccattg
    24481 aaggtggaac ccgattcatg agcaaaaatc agtgtttgtt gaaataattc tgttgaaaca
    24541 cccccactca aaaaaatgaa tggtaatgga gtcgcttcac tttgctcctt aaagaaggca
    24601 gcagcctctt ctttgctgtg aataaattca cctgtggaat agccattcac ataattcata
    24661 ttgactggta cttctacttt caacacatca actttatatt ttggatcaga aaattctttc
    24721 atcatctcaa taactttgtg gggcttgact ttcgcatact ctggagaaga actatcgctt
    24781 atttgtgcgt cataagaaac taactccaaa tagaagggac gattttctcc ttcgcactca
    24841 ctacctaaac gttcgatgaa cacatgcttc aaatgattga tttcgggtgc ttcatcaaca
    24901 tcgtagtaaa gtaagaattt tactgcatcc gcttgctgtt ctttcaatct gagagaagac
    24961 caatccgcta ataaatcagg tagacgacca ggcgtagttg cgtcatagcc ggttttctca
    25021 taagctaaca ataaccccgt ctcttttgct ctgacttttg ctgctggcaa tccatattcc
    25081 ggatccaata aaatagcaga agcatagggt gtcagctcag ctgatactag ttctttgaat
    25141 tcactgattt ggtcttcact tggctctttt cccaacgatt tgatcatttt ttttagagca
    25201 ccacgctgat cgatcgctaa tgcactaata atcccatctt ttgttgatag tctctccatt
    25261 gccctatttt tttcttcatt catctgtcat ttctccttcg gtttcatact tatgaataat
    25321 gacgcccttg actactcgat tgaccgtgcc tgttggagat ggtgtatctg gtttattagc
    25381 tactttgatt gaagtataga gtgcaatgat ttgtgcagtc ataatcatcg ctaatgcaag
    25441 ataggcatcg ggcaatttac tcaccgagtc ttccaatagc acagtcatac ctgagaaatc
    25501 aatagtaccc ggttgcgcaa tcgcaatgac attggcagtg acccgatctt cttttatttc
    25561 ctctaaaata tctaagtcat aatttcgcgt atatggttga ttgctcacaa aacctacaac
    25621 gagtgtctcg ctatttaaaa aagactttgg tccatgacga aaccccatcg aagaatcaaa
    25681 aattgtagca actttacctg ctgttaattc taaaattttt aattgtgctt ctcttgtcgc
    25741 acctgccaaa cttcctgaac ccagataagc gatccgttca aaaaaatcag gtaataactt
    25801 ttgaatttct tgatctcttg aaagaagatc ttgaccgatt ttaactaatt gttcaagata
    25861 tctttctttt ttctccattg ctgtttgatc aaagatccat aaagcactca ataacataca
    25921 cgtaaaactt cctgtcatag caaaacctgc atcgtttgct ctctctggca tcaataacag
    25981 ataattctgt ttatcatcct gtgcgttttt tgccaacatc ccctctttag cacaagtaat
    26041 cgtcaaatga aacaatgttt caacttgttc atttgcaatt tgcacagccg ctacactttc
    26101 tggactgttg ccactgcgag caaatgaaac taataaagtc ggttcatttg gaaacaaaaa
    26161 ttcttttggt gaagctacaa tatctgtcgt gccaaaactg tggaagataa aaaactgatt
    26221 gtctccatgt tcatttagat agggtaagag cgtatcccca atatattgag acgttcctgc
    26281 acccgtaaaa ataacacgta ttcttcttcc gtttgcttct ttttttattt gatcaaaaaa
    26341 tagagctaaa ttttcttggc tttgcatcaa tctatcgatt gtttctttcc agagatctgg
    26401 ttgttgtttt atttcttgtg tcgtgattgc tgcacctatt tgttttaatt tgttcttcga
    26461 tagctcaaac atgttgttct ccctctcaat cattttcgga tatgccgaat tttataatga
    26521 aactggtccg ctcttgctac actatctgta tattcgataa tctcattttt cacgttatag
    26581 gttatcctcg tcagatgtaa gacgggagcg ccatcagcga ttcgtagcac aacagcatct
    26641 tttttttgca cgatacttgc ataaaactct tcatcagcta attgaacttg ttgttcataa
    26701 tcctgtgaaa acacatcata caacggttta ctttcaagta acggcaaatc aagtcccata
    26761 aatagtttcg ccggtagata agaacgctca accatcaatg gctcttcatc tgccaaacgc
    26821 aatcttttaa gtttaaatac agtttcacct aaatggatgt tcaaattctt agcaattctc
    26881 ttattagctt ccatttgtct aaattctaaa atgaccgtct tagggacgcg ccctatttcg
    26941 cgcatttctt ctgtaaagct atatgctcca gctaagttcg atgacttatc agacaaatca
    27001 gagacatagg taccttttcc atgtcttcgg taaatataac cttgattttc caattcctgt
    27061 aaagccagac gtacggtggt acgactcacc ccatagtgtt gacaaagttc tctttcagat
    27121 aacagcttat catgcggagt catctgtgtt tcgatacgat ccttcaataa atcaactagt
    27181 tgataataca atgattttcc tttatttgag ctctccataa aaatcacctc ttctttactg
    27241 gttataacca caacacaaga atagtgaatt attaatcatt tgtcaaaggt tttttagacc
    27301 aaaaaagcta ttataacagc tctttaaagc tttatatctt gaatagacaa agatacctca
    27361 aaaataccag ttaccaataa aagaacagaa aaaaagacga accacataag cgatccgtca
    27421 aaatggtcta acttatcgag cagcgtgatc cacattaatc cctatgatat tcctacaact
    27481 atcgttttat tacttccaat atcatttaca acaactaaaa atagcttatt aatgacaaat
    27541 gttattatta gttttagata acttttgcag ttttatactt tttttctata aaatttgaca
    27601 aacatttttt taaataacta attatttttt gaatctgtca ctcagacaat cctttctctt
    27661 acttgttaac ataaatagaa gttgctgttt cttctaatag tgtacttaat tctttaatat
    27721 tttgttttcc ttcttctaac atccaactac tcgctttttc ttcgccttcg gtaacaaaag
    27781 gtgctactcc cttcgcccat gttgctcgcc cacataagac accattgaat gtcgaacctg
    27841 attgttttgc aaaacgcaat gtatcttgaa atagtgcagc gctgactccc gcacttaaaa
    27901 aaataaacgg ttgttcagtc actttcgatt gttcatagaa atacgcggat gcctctgctt
    27961 gactataaca agtatcttgc ccatatcctt ccacaaatct catattcact ggtacttcta
    28021 ctttcaatac atccacttga taacgttctt ttgaaaattc tcgcatggct tcaatcactt
    28081 tatgcggttt tacttttgca tagtcaagag aagacggatc gccaatagtt gcatcgtaag
    28141 aaaggagctc taaaaagaaa ggtatgcctt ctgcttcgca ttctgaacca atccgttcta
    28201 taactatttg tttttgcaca ttaatctcta ctggttcatc aacgtcatag tacaataaaa
    28261 atttacaagc atccgcaccc acttctttta accttttcac tgaccaacta ttcagtaaat
    28321 ctggtagtcg gcctggcatt ttcgcatcat agccggtttt ttcataagct agcaacaaac
    28381 cacattcttt ctctcgaagc gctgctgcag gcagaccata ttctggatcg agtaaaatag
    28441 cggaagcata cggagtcagg tactgagaaa ccatctgttt aaattggatg atatcttctg
    28501 atgtagcttc tttatattgg ttgatcattt tcttcaaagc accacgttgg tcgatagcta
    28561 gcgcgctaat aattccagct tcatccgtca atcgcttcaa acgttcctgt ttttctatac
    28621 tcattttctt catatatctt cacctatttc tttagttaat attgtagcta tttctatttt
    28681 tgattttgca gctctcaact gttctgtaac ctcttttcgc atcaatgatc gtgcaacgag
    28741 agacaataat ttcaaatgag tcgttcctgc ttctgaatct gggataaata acgcaaaaat
    28801 aaaatcaact gactgtctat ctaaactgtt ccattcaatt ccttttttca gtgtgacgat
    28861 aatcaaacta ggttcattga ttgtttgaga cttcgcatga gggatagcaa aacctgacat
    28921 tattcctgta gtaccttctt tttctctttc cagaagtttt tgatatactt ctttttcgtc
    28981 atgagcgatt cctaaacaga atgtttgctt tgctaaaaac ttaaatgctt cttcttgatt
    29041 atctaatggc tgttgtaaaa aaatgttctc ttcatgaatg attgtcatga taaacctcct
    29101 gctcttcctt cttcgataac tgtacgattt gttgctttat tatccctctt cttgtcttta
    29161 tcagttactc ttttcttttg gatcaaacct agtatcactc caccaacgat cgaaccaata
    29221 agaatcgctg taacccattg gatttttccc gtcactacag gtaacactaa aaaaccgcca
    29281 tgtggggctg gtacttggac accaaacaga taggtcaaga cagcagcaat tgatgagcct
    29341 aacatcatcg ttggtaatac tcggatcggg tctttcgcag caaacggaat agctccttcc
    29401 gtaatatgag ttgatcctaa aataaaatta accaaacctg cattacgatc attagtatca
    29461 aaatatttac caaacaataa agtagcaaaa ccagtaatca acggtggtgc aatgcaagct
    29521 gctgacgcac cagccataaa agtcgtgttt ccttgtgtca aaagagctgt tcctgttaca
    29581 tatgccgcct tattaaccgg tcctcccata tcaaacgcac acatacagcc aacaatcacg
    29641 ccaagaacaa gtggattcga atattcaaat ccagctaaaa agctcatcat tccttcattt
    29701 atagccttca ttggacctgc cagcaaagac atcacgatgc caataaatgc tacaccaata
    29761 actggatata agacgattgc cttcaagcca tctagttgtt taggtaaaat tatcagtagc
    29821 ttttgcaaaa ataagataaa ataacctgct aagaacccag ctaaaatacc tcctaaaaat
    29881 ccagtaccgc cattcatggc tacgattcca ccaacaaatc caacgactaa ccctggacgt
    29941 ttcgcgatcc cttctgcaat ataagctgct aaaactggaa ccatcattcc cattgctgca
    30001 tcgtcaactg cttttaccat tgccgctgta gcattgtatt gtgcatgatc tggatcagaa
    30061 gaatagattc cccaaacggc aaatgaaata gcaatcaata ctccgccagc taccacgaaa
    30121 ggcaacatat gagagacacc gttcatcaaa tttttataga tgctacgacc aaaattagca
    30181 ccacttatcg cttctggttc taaagtatca gcagcttcat tatctgctaa gacagtacct
    30241 tttcctgcca atgcttcgtc aatcagccgt tgtgcatcct tgattcctgt actgacagaa
    30301 acatcaatga ctcgttttcc cacaaatctt tctgaatgga catctttgtc tgcggcaata
    30361 atgacagcat ctgcctgttt gatttcttca gccgttaatt cattctcgat cccaatttgt
    30421 ccatgggtct ccactttgat cgtgatgcct tttttcctag ctgcttgctc taatgcctct
    30481 tgcgccatgt aggtgtgcgc aattcctgtc ggacaaccgg ttgctgcaat tagttgatat
    30541 tttcccatct ttcgaacctc tcttccttga ttttgatttg tttacttaat tcctccacat
    30601 ccaaaaaatc agtcagtcct ttacgaaaag cagtagagct gcctgctgct atgcttttac
    30661 gtaacgtttc atcaagcgat cgtcgattca tgtatcctgc taaaaaagtg cctaacatgg
    30721 catcacctgc acatgctgta ttgacaactt ttccttttgg cgagtttcct cgaatcaccc
    30781 tgtctttcgt aaaaagaatc gctcctttct caccgaggga taacaaaaca ttttctgcgc
    30841 ctgactctac tagtcgttgt ccataaacaa agtaatcttc atctgtaacc atttttcgtc
    30901 cgaaccaaga agaaagttct tcctcatttg gtttcagcaa aaacggtcga tagggtaaac
    30961 aatccatgac ttcttgatca ctactatcaa taatcaaaca aatccgactg atttctatta
    31021 aaacctttgg agacagccct cttggtaaac tcccagaaac acaaagataa tcccctgctt
    31081 gtaatgctct tatttgattt aaaaaattat gaacagctgt ctgcggtatt tcaggtcctt
    31141 gattcactaa tttgtattct tctcccgttt gattgacttg tgtaaataca ttgatacgcg
    31201 tcataccagg cacttcaata aactctgttt ggatttgttt ctgctgtaaa tagtcttcta
    31261 tatactttcc tgtgaaccca gcagaaaaac ctaatgccgt acttgggaaa tctagcatct
    31321 ttaatactag tgacacattc accccttttc cgtttgcctg aatgtcatcc tcttttgtgc
    31381 ggtttaccat aaaaggatgc atctcatctg tttcaataaa tagatcaatt gccaaattca
    31441 tcgtgcatgt gtagatagac ataaccaacg acctccttga ttctattatg aaatacgcga
    31501 aatagaaaat gttttcttaa tcgtcccaac acaaagaaaa cctttacatt tataaactcg
    31561 tcgtgctttt tttaactaac ttttggtgaa agaaggaaag aatatgaatc acaaattaag
    31621 tgaaacagag cagtacctct ggaactttat cgaacacctc atcctagaga tccctaacta
    31681 ttccatcgtg aaactaagtg aacaagcaaa tgtctcaaca gccaccattg tgcggacaat
    31741 gaaaaagaaa ggctatgaag ggtttacttc gtttaagcat catctaaaag aaggagaaaa
    31801 caagaacatc aatttttcat ttttagataa agtagacaaa ggaatccgcc gagccattct
    31861 aaaaaatgaa caagaagtgg tacgtacaat caatatgttg gaaatcggga acattgaaga
    31921 tgcaatacaa agaatcaaat ttgctgaccg ggtttttgtc tttgcccgag gattttcaga
    31981 aatgattggc caagaaatgc tggtaaaact tcaattaacg ggaaaaaatt gtcagatgca
    32041 tactgatcca gaaattatca aaagcatcag tcggaaacta actaagaaag acagcgttat
    32101 ttttgtttct ttgaacggtg agaaaacaaa taaaaacatc aaaaataaga tttatgtctt
    32161 tatttttcaa ttttttaaat ctattatttg tcaaattaag cgagacaaaa ctttccattt
    32221 acgtaactca aaaaaacttc taatggtgtt tggtaattta gtgattttct agggatatgg
    32281 tttcttttgg acgcaacgga tgagatgaat ccttgattga cttggttgaa ttccatttct
    32341 tttggtagtc catcttttcg gaggagtccg tttgaat
//
LOCUS       DACGLH010000027        31067 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.029, whole genome shotgun sequence.
ACCESSION   DACGLH010000027 DACGLH010000000
VERSION     DACGLH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.029"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(287..649)
                     /locus_tag="IXT21_001357"
     CDS             complement(287..649)
                     /locus_tag="IXT21_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868768.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IXT21_001358"
     CDS             complement(723..1103)
                     /locus_tag="IXT21_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ4868769.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IXT21_001359"
     CDS             1185..2360
                     /locus_tag="IXT21_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4868770.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IXT21_001360"
     CDS             complement(2437..3021)
                     /locus_tag="IXT21_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4868771.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IXT21_001361"
     CDS             3222..4220
                     /locus_tag="IXT21_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4868772.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IXT21_001362"
     CDS             4332..4841
                     /locus_tag="IXT21_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ4868773.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IXT21_001363"
     CDS             complement(4871..5530)
                     /locus_tag="IXT21_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ4868774.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IXT21_001364"
     CDS             complement(5532..6296)
                     /locus_tag="IXT21_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ4868775.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IXT21_001365"
     CDS             complement(6304..6981)
                     /locus_tag="IXT21_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ4868776.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IXT21_001366"
     CDS             complement(7018..8403)
                     /locus_tag="IXT21_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ4868777.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8372..8626)
                     /locus_tag="IXT21_001367"
     CDS             complement(8372..8626)
                     /locus_tag="IXT21_001367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868778.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IXT21_001368"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXT21_001368"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IXT21_001369"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IXT21_001369"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ4868779.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IXT21_001370"
     CDS             9342..10199
                     /locus_tag="IXT21_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ4868780.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IXT21_001371"
     CDS             complement(10280..10615)
                     /locus_tag="IXT21_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868781.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IXT21_001372"
     CDS             complement(10682..12052)
                     /locus_tag="IXT21_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ4868782.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IXT21_001373"
     CDS             complement(12130..12666)
                     /locus_tag="IXT21_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4868783.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IXT21_001374"
     CDS             12975..14456
                     /locus_tag="IXT21_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4868784.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="IXT21_001375"
     CDS             complement(14559..15266)
                     /locus_tag="IXT21_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ4868785.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="IXT21_001376"
     CDS             complement(15345..15683)
                     /locus_tag="IXT21_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ4868786.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="IXT21_001377"
     CDS             complement(15794..16756)
                     /locus_tag="IXT21_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ4868787.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="IXT21_001378"
     CDS             complement(16771..18102)
                     /locus_tag="IXT21_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4868788.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="IXT21_001379"
     CDS             complement(18110..18790)
                     /locus_tag="IXT21_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ4868789.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="IXT21_001380"
     CDS             complement(19009..19488)
                     /locus_tag="IXT21_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ4868790.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19615..21294)
                     /locus_tag="IXT21_001381"
     CDS             complement(19615..21294)
                     /locus_tag="IXT21_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ4868791.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21842..23344
                     /locus_tag="IXT21_001382"
     CDS             21842..23344
                     /locus_tag="IXT21_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868792.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23357..24019
                     /locus_tag="IXT21_001383"
     CDS             23357..24019
                     /locus_tag="IXT21_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868793.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24120..24323)
                     /locus_tag="IXT21_001384"
     CDS             complement(24120..24323)
                     /locus_tag="IXT21_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ4868794.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IXT21_001385"
     CDS             complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IXT21_001385"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ4868795.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IXT21_001386"
     CDS             complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IXT21_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ4868796.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28287..28901)
                     /locus_tag="IXT21_001387"
     CDS             complement(28287..28901)
                     /locus_tag="IXT21_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4868797.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29507..29884)
                     /locus_tag="IXT21_001388"
     CDS             complement(29507..29884)
                     /locus_tag="IXT21_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868798.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30184..31065)
                     /gene="galU"
                     /locus_tag="IXT21_001389"
     CDS             complement(30184..31065)
                     /gene="galU"
                     /locus_tag="IXT21_001389"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ4868799.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         9268 a         6456 c         5185 g        10158 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcgtat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt
    19681 ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc
    19741 aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt
    19801 catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga
    19861 aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga
    19921 caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat
    19981 cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag
    20041 cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa
    20101 atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt
    20161 catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt
    20221 ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac
    20281 gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat
    20341 cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat
    20401 tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt
    20461 aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat
    20521 tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa
    20581 tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca
    20641 atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg
    20701 attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc
    20761 caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc
    20821 cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt
    20881 atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc
    20941 taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc
    21001 taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg
    21061 aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat
    21121 gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc
    21181 aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat
    21241 gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt
    21301 cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat
    21361 gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag
    21421 gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt tggatatact
    21481 tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca
    21541 gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa
    21601 caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc
    21661 atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac
    21721 tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt
    21781 taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa
    21841 tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt
    21901 attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag
    21961 cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag
    22021 tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac
    22081 tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga
    22141 aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat
    22201 ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac
    22261 tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc
    22321 agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac
    22381 agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt
    22441 cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac
    22501 gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag
    22561 cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta
    22621 cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc
    22681 tgctatttac actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa
    22741 gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt
    22801 ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc
    22861 cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt
    22921 aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga
    22981 agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc
    23041 gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca
    23101 agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg
    23161 tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat
    23221 caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt
    23281 ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa
    23341 ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca
    23401 agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt
    23461 aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc
    23521 aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta
    23581 tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc
    23641 tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca
    23701 agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt
    23761 gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga
    23821 acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga
    23881 tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac
    23941 tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt
    24001 tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct
    24061 gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt
    24121 tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct
    24181 ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg
    24241 tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta
    24301 gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct
    24361 tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag
    24421 ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt
    24481 ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga
    24541 tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac
    24601 gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca
    24661 cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat
    24721 gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat
    24781 ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat
    24841 ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt
    24901 tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa
    24961 attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt
    25021 ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac
    25081 gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg
    25141 atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag
    25201 aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc
    25261 cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt
    25321 ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga
    25381 cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac
    25441 agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct acttccactc
    25501 cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta
    25561 catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac
    25621 atttttcctc tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat
    25681 aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg
    25741 tttggattac ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa
    25801 tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg
    25861 cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg
    25921 gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg
    25981 atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg
    26041 aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa
    26101 tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg
    26161 aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta
    26221 cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga
    26281 ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa
    26341 agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc
    26401 agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat
    26461 tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg
    26521 aactttctgg ttttttacgg attttttctg catatatcat catataacca gcggctaatg
    26581 cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag
    26641 caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt
    26701 gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa
    26761 atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac
    26821 cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac
    26881 tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac
    26941 ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata
    27001 ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata
    27061 ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt
    27121 aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc
    27181 cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg
    27241 gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat
    27301 aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt
    27361 attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa
    27421 agatgactac accaaaaata atgacatacg tatggggtgt cgataattta gtgaagtcgc
    27481 gtttcggttt tcctgaagaa tttgttttct tcaaatttat ttcttccttt cttttttcag
    27541 taattttgat gaataaaaaa tggataatca aatgaattat acagtaaaaa tgacatttcc
    27601 acatgaataa tttattagaa aatcactttt ttctattttg ttttttttct cctatgtatg
    27661 attatttttt tctctttcca tttatattaa gtattttatt tctctgtttt tccttaaaat
    27721 tcttttttta tttttagatt agagaaaatc tcgtcctgtt tttttcattt tacaatcatt
    27781 gaaaaattga atttcattgc aaaaaaaata ataaacactt ataaatatcc attatctatc
    27841 cttcaatttt tgtgtatttt atttctatta gaaaaaggta tcctgttgga caattttgtt
    27901 atatcaggta aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga
    27961 gttttgggga agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat
    28021 tctttcttgt ctctttttcc actacttttt atttttctga cactacatat tcttataact
    28081 catttctcat aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta
    28141 aaatcaaaga tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct
    28201 cattaataat cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt
    28261 aaggaacttt gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt
    28321 gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag
    28381 atcgctggat ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc
    28441 aaaaccaaat cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt
    28501 cgtgacaaat caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga
    28561 gctagttcga tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct
    28621 gatttgacag aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca
    28681 cctccggctg gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat
    28741 tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta
    28801 atttgtttcg cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc
    28861 tcttccaatt ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct
    28921 atacttgaca tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt
    28981 tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac
    29041 gcccaatacc ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag
    29101 gggtagaatt ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta
    29161 tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc
    29221 aactaagggt cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta
    29281 atctcttatc caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat
    29341 ataaataaca taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag
    29401 attcttttat ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa
    29461 tagtttatcc attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc
    29521 tcgttcttct actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt
    29581 ttctgcctat ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag
    29641 caaaaaataa agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg
    29701 tagcaacccc aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga
    29761 ccatgcgaaa catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc
    29821 ttccttccat cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc
    29881 ccatagtgaa cacacacctt tttttataat taaaaaattc gcctttttat tctactatga
    29941 atcttctggc ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa
    30001 aaaattaaac cattagcata atattttctg aatctattca cgaaagtctt tttctatctt
    30061 tcgttttcca cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg
    30121 acgcacaagc tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc
    30181 cagttaactt tctgttctta gctttttact caactggata atatatgcac ttaatgaagt
    30241 cccaatttcc gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc
    30301 gcctacgtcg tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag
    30361 agtctgaagt gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa
    30421 tatctcaaat attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc
    30481 ttttcccatt tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc
    30541 tgttttagga tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc
    30601 aacagcggaa gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc
    30661 atctttcatc aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt
    30721 tgcttgaagc acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt
    30781 gacttgagtc gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt
    30841 gttttccaat tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt
    30901 tacgatcaag atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt
    30961 tggtttatcc acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg
    31021 tgttcctagc ccagcggcag gaatgactgc ttttttgacc ttcactt
//
LOCUS       DACGLH010000028        27634 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.030, whole genome shotgun sequence.
ACCESSION   DACGLH010000028 DACGLH010000000
VERSION     DACGLH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.030"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..262
                     /locus_tag="IXT21_001390"
     CDS             <1..262
                     /locus_tag="IXT21_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAQ4868800.1"
                     /translation="KVDIEKWADTIRLMEERDKASIEAIEYVINWLPTNEFWFGNIRS
                     AKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI"
     gene            280..1131
                     /locus_tag="IXT21_001391"
     CDS             280..1131
                     /locus_tag="IXT21_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4868801.1"
                     /translation="MESLANAMEKLIRRVLVQSGRCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWKILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            1128..1487
                     /locus_tag="IXT21_001392"
     CDS             1128..1487
                     /locus_tag="IXT21_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ4868802.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANDWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            1502..1663
                     /locus_tag="IXT21_001393"
     CDS             1502..1663
                     /locus_tag="IXT21_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ4868803.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            1660..1986
                     /locus_tag="IXT21_001394"
     CDS             1660..1986
                     /locus_tag="IXT21_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868804.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLEKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            1983..2798
                     /locus_tag="IXT21_001395"
     CDS             1983..2798
                     /locus_tag="IXT21_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ4868805.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            2937..3248
                     /locus_tag="IXT21_001396"
     CDS             2937..3248
                     /locus_tag="IXT21_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868806.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            3245..3475
                     /locus_tag="IXT21_001397"
     CDS             3245..3475
                     /locus_tag="IXT21_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868807.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            3482..3685
                     /locus_tag="IXT21_001398"
     CDS             3482..3685
                     /locus_tag="IXT21_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ4868808.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            3682..3987
                     /locus_tag="IXT21_001399"
     CDS             3682..3987
                     /locus_tag="IXT21_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868809.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNGIKN"
     gene            3971..4264
                     /locus_tag="IXT21_001400"
     CDS             3971..4264
                     /locus_tag="IXT21_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868810.1"
                     /translation="MELKIDKVTLEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEHQK"
     gene            4525..4941
                     /locus_tag="IXT21_001401"
     CDS             4525..4941
                     /locus_tag="IXT21_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ4868811.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKTPKHGNKTEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            5647..6078
                     /locus_tag="IXT21_001402"
     CDS             5647..6078
                     /locus_tag="IXT21_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868812.1"
                     /translation="MDSLDFLQNNVVKELKEKGFELEDIQKMTFEDIANNLYYPKFRL
                     EVDSQYGESVLYASEKVVSEKDSFMYTLPNGTLIITKPALSKYIERFVVESRKQHSEF
                     GKPFYIKKRNEPWDQDEECLEYERSILKKRDEKPGPPIRKD"
     gene            6083..6310
                     /locus_tag="IXT21_001403"
     CDS             6083..6310
                     /locus_tag="IXT21_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868813.1"
                     /translation="MRNYWYVSLTNRYPQPNADDPIRVVQSVQIKKKYSIVEMTREAT
                     PKEIDKYNLRYCGHGYWKDEYIQQNIERYIK"
     gene            6307..6591
                     /locus_tag="IXT21_001404"
     CDS             6307..6591
                     /locus_tag="IXT21_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868814.1"
                     /translation="MNDNYDYIKLIEKIRAEKDMDELATLFMNIISLVGLKMDEVAAL
                     NYFIAEQTIRAEHNAEFLKDRLDLDVKGLGVEGIFKVQEALVNVYVEKMQ"
     gene            6632..7204
                     /locus_tag="IXT21_001405"
     CDS             6632..7204
                     /locus_tag="IXT21_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868815.1"
                     /translation="MKLKDYIREGYNIVTTPNLAYKIQEDYPDALVFTDRALDAIPIG
                     KLLVDRYYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWHTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            7205..7516
                     /locus_tag="IXT21_001406"
     CDS             7205..7516
                     /locus_tag="IXT21_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ4868816.1"
                     /translation="MIDVSTKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPNLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            7507..7767
                     /locus_tag="IXT21_001407"
     CDS             7507..7767
                     /locus_tag="IXT21_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868817.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            7935..8489
                     /locus_tag="IXT21_001408"
     CDS             7935..8489
                     /locus_tag="IXT21_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4868818.1"
                     /translation="MIGLNSKLVGTRMKQKRIEKGLTQADVATKSGISSRYYGSIENG
                     KNSPSIETLNSISIVIGCSLHFLLNDRMDDMEKRVELEKKRLQKIFDKIPHDKLNLVE
                     GLIIQAARLRVLLDDNWKDIIENGEYEKFKQSENQIAYDRKRPIVENYDNRDKTYQAI
                     VKQLTDLLPQGTRADKKSKLLGRK"
     gene            8492..10183
                     /locus_tag="IXT21_001409"
     CDS             8492..10183
                     /locus_tag="IXT21_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ4868819.1"
                     /translation="MLFNQHVDQYIRKWKSGELILNEKRIQLLKLIEKEILPRDDIYY
                     FDEEQINNYIEFSQAWYFELDEWEKFISAFIFLFYREDDEVVFDEFVINMGRGGGKNG
                     FISTLANYFVSSLHGVDYYDVSIVANSEKQAKRSFQECYRVINKKGNEDLKEEFEAYK
                     SSLTGLETQSVFEYKTSNASSQDGGREGAVIYDEYHEMENTDIVDVFSGGLGKVDHGR
                     QFFIGTKGFVREGYFDIKYRECEDILNGLSEFKGVFPYICELDAIEEMDDPKNWPKAN
                     PALQPPLNKRGKRLFNEVMKKYKKLAKEPSGRSAFVTKRMNFLEDNMENSVASREEIM
                     ATNRPFFELDTVPIGSLDFGSVRDFATCGLLFKNRKKSEYAFKSFTFAMKNFCDVHYG
                     YSLKEQLVGSEKRAPIKTWEKMGLMKVIDEPSLNPKYIVDWFIQAREEYGVRVIVVDN
                     YKADILGPMLEKEGFEVVRLKRPSSLHPLLAPRIEDGFANHKFIFGDNPLMRWFTNNV
                     YVKETQAGKQFLKKEEVKRKTDGFQAFVHALYKATELDEQIDYDDAFDMLDEIEF"
     gene            10254..11081
                     /locus_tag="IXT21_001410"
     CDS             10254..11081
                     /locus_tag="IXT21_001410"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868820.1"
                     /translation="MLGILAFTFVAIKLSEIIIPNDSVVSKNLVLERAKLIISILGPA
                     LTLIVFVRTIQTQLESSKNLIKDNVSKDFYNLLNLFKSIQDKMNSELFSTIEKRIKVP
                     INNINTNLYVRENKETTKEVQEDLKEYSASLNKALSESVKKINEIFEENYKDIGSYLK
                     VFHRILKILNNRYESKDISHEEYKMYVGILRSQIEDYEFYIILINSLFTLRGTGMGIE
                     LIETSFFGDELDIDLKQHFEHPEFLTEIIKDNFVEKKSYRESIKTRKKIGNYLKIER"
     gene            11210..12382
                     /locus_tag="IXT21_001411"
     CDS             11210..12382
                     /locus_tag="IXT21_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ4868821.1"
                     /translation="MSLFDIFKQSIKNEDPSDWIPDFVFGDEESARAYLKIMAKSTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWAMSDVIYMEYNNEELERFTR
                     GLFEDYAELFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDKIW
                     NSFKTNSVAIVPKVKGFKYEEYTNKQGVSNQSLDELNKMKSSLIADVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGVRIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            12369..13073
                     /locus_tag="IXT21_001412"
     CDS             12369..13073
                     /locus_tag="IXT21_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ4868822.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWCDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            13078..14238
                     /locus_tag="IXT21_001413"
     CDS             13078..14238
                     /locus_tag="IXT21_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ4868823.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPINNDAAQVYDLDFTAGE"
     gene            14314..14592
                     /locus_tag="IXT21_001414"
     CDS             14314..14592
                     /locus_tag="IXT21_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ4868824.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            14573..14944
                     /locus_tag="IXT21_001415"
     CDS             14573..14944
                     /locus_tag="IXT21_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ4868825.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            14960..15364
                     /locus_tag="IXT21_001416"
     CDS             14960..15364
                     /locus_tag="IXT21_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868826.1"
                     /translation="MSNVKFEGVQEIIQALEKKFGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            15361..15771
                     /locus_tag="IXT21_001417"
     CDS             15361..15771
                     /locus_tag="IXT21_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868827.1"
                     /translation="MKDMLNIIYEALCLNEYIRSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            15832..16419
                     /locus_tag="IXT21_001418"
     CDS             15832..16419
                     /locus_tag="IXT21_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4868828.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQDKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITALKEMVFPAAGK"
     gene            16492..16959
                     /locus_tag="IXT21_001419"
     CDS             16492..16959
                     /locus_tag="IXT21_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAQ4868829.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            17005..17313
                     /locus_tag="IXT21_001420"
     CDS             17005..17313
                     /locus_tag="IXT21_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868830.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            17508..24242
                     /locus_tag="IXT21_001421"
     CDS             17508..24242
                     /locus_tag="IXT21_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ4868831.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNERAIGGT
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            24246..25118
                     /locus_tag="IXT21_001422"
     CDS             24246..25118
                     /locus_tag="IXT21_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868832.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            25115..25742
                     /locus_tag="IXT21_001423"
                     /pseudo
     CDS             25115..25742
                     /locus_tag="IXT21_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25703..26164
                     /locus_tag="IXT21_001424"
     CDS             25703..26164
                     /locus_tag="IXT21_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868833.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            26168..26992
                     /locus_tag="IXT21_001425"
     CDS             26168..26992
                     /locus_tag="IXT21_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868834.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            27010..27555
                     /locus_tag="IXT21_001426"
                     /pseudo
     CDS             27010..27555
                     /locus_tag="IXT21_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT        10007 a         4136 c         5980 g         7511 t
ORIGIN      
        1 taaagtagat atcgaaaaat gggcagacac aatcaggttg atggaagaaa gagataaagc
       61 atctatagaa gcgattgagt atgtgatcaa ttggttacct acaaatgaat tttggtttgg
      121 aaatattaga agtgctaaga aattgagaga aaaatttgag aagctcaaat tcgaaatcaa
      181 agcagataag aagaatcata aaaagcaaag tcaaaaacta cagtacagca atcctagtga
      241 atatgacgac ttgccaattt aaaaaaggag atgcatcaca tggaaagcct agcaaatgct
      301 atggagaagc taataagaag agtattagtg caaagcggaa gatgtccaga atgtagcgaa
      361 cctttgtata gttggcgagc taaaaataag gatggttcag aacgttgtaa accaacatgc
      421 atgagttgtg gttataaagc gttacgtgtg aaagaggata tacagaccga acggatatat
      481 aacgacagct taaaagcacg agcgttgagt ttttttcaaa atggttcggt attaacagat
      541 aaaactttgt ttaaatgcaa aatggagaat tatcacgtag tggaccaaga aacgaaaatt
      601 gctttagaaa gagctaaaag ctatgtaaat gatgtcctac tgaaccatcc tgcacatttc
      661 attctatcag ggaaatcagg aagcggaaaa agccacttat caatggctac agcttggaaa
      721 atacttgagc gctcaaatta tgacaagaaa atacttttta taagctatca agagttatta
      781 gagcaaataa agttttccta taacaatgct gaactgagaa aagaaattga aggatcgctt
      841 atagccgata tcaaaacaac tgatttggtg gtttttgacg atattggagc tgaattaggt
      901 agcggggtat caaatagtag gcagtttaca aacaatacgt taaacacgct cttggaagcc
      961 agacagaaca aggcaacgat tatcacaaca aacttatctg gtcccgaact aagagaagcc
     1021 tacggcgaaa gaattgtttc taggatattt aagaattcag aaggttatgc gctgaaattc
     1081 caacaaacag cagacaagcg cataaaacca gtgaaaggta gtatcgcatg aataaatacc
     1141 gtaataagaa aactgttcat cgaggtatca agttcgattc tatcgcggaa gcagagtact
     1201 acgatctagc cttgtggcaa gcggaagcga atgactggaa agtaaagctt caagaaagat
     1261 ttgagctgat gccgaaattt gaactcgaag gcaaaaagta tcgcaaaatc gagtatattc
     1321 ccgacttcac attttataaa aatggcaaac ttgtaaaagt cgtagatgtc aaaggaatgc
     1381 agacaaaaga ctttaagatc aaggcgaaat tgttttgtca tcaatatcaa gttccattga
     1441 tattagctaa aaaatatcgg aatacgttca aggaagagcg tttttaaaga ggtggtccat
     1501 catgacaaca gaagaagtga ttcaaatgcg tattcgaagc attcaacgtg aaattgacga
     1561 tctggaacga acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa ttgatttgca
     1621 cattgagaat ttaagaaggg aaattcgtag attggaggaa tgagcgtgga taaggaagcg
     1681 gcttggcgaa aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg
     1741 gttgctgaag ttcagcgtct agaaaagatt gctaacggac gtatacgaaa aaagccagac
     1801 aaaagaaaac agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg
     1861 caaggaacag ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg
     1921 tacgcttggc gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa
     1981 aaatgaacga aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg
     2041 gagttatcgg aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct
     2101 attcaatgaa cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga
     2161 ttgacaaagt gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag
     2221 tatctacaag cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt
     2281 caactaaagc agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa
     2341 gtggatggtt aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc
     2401 tgcttgatgt cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg
     2461 ctaaatcagt tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg
     2521 tcgataaaga aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa
     2581 aagcgaagct agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa
     2641 caaaagctgc agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca
     2701 atttgattcg ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg
     2761 gagcagatac tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa
     2821 attctcttgt tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa
     2881 actgatgaca aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg
     2941 acgaactaat cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc
     3001 caaaagcaca gtttttgaaa gtagctgagg aattcgggga aatagcttca gcgatggcaa
     3061 gaagtaatga tgagctattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc
     3121 tttccatgca aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca
     3181 aaggacgcac agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggaggatg
     3241 tgaaatgaaa tacgaaatac cactaagtga agcgggcatt caagcaatta tcaatggtcg
     3301 ggaggttaac atagaacttc ctgatggtac tgaattagtc atcagacaaa gttatttgaa
     3361 agatatggca gctccagtat taattgatcg ttttaacgtg actgattctg tggtagagaa
     3421 ccacttaaaa gaatttcgat caagtataga caacactttc agattaggga gttgattgac
     3481 aatgaacacc agacaccgca gagtagcaaa actaagaaaa caggaactaa atgtactaaa
     3541 gacaaagttt gaaaaagaat atggaatttc agcagaagaa acatataaag tggcaagtca
     3601 gtttgttgct gatgcaagtg atgctattcg taagtttggg atttcgatat taaatgatga
     3661 tcgtaaatgg gaggaaatga gatgaaacta aaagacggat tttacgctag tagtcacggc
     3721 atcggcggtt taatgctaga tatgccgaca aagaacccta aaacacgtaa gaaaccaaaa
     3781 gtcaaagtcg gtgacatggt tcgctgtgaa gcagaagagt tcatttatcc gtttcgtgga
     3841 tatgtagaac acgtctataa tcactcagcg atcattcgta ttgaaaacac aatggaatgc
     3901 gataagtggt tagcgaaaag caaagagaat ttagcagtgg cgagattggt ggatatggag
     3961 gttataaaca atggaattaa aaattgacaa ggtaacgctt gaagaaccag catttcaaga
     4021 aatggatgaa cgaattgcag aattggtcga gaaaaaacaa gatgaattga ttgctttgct
     4081 agaaaagaaa taccaatttc cgaaatatat gaacaaaatg caagcagcaa aatatcttgg
     4141 tgtaagttac aacacgatga tggataagta cgtaccagct ggtttgaaaa tgggtatcgt
     4201 taacggcgtt attcgtatca gcaaagaaga atgtgatcgt tttatgaaag aacatcaaaa
     4261 ataaaataaa aaaacgttta gccccttagc taaacgcaca attataattt acctgacagt
     4321 taattataac aaaattgcgg aggttgagga atggcggaat gaaaaagaca ataaaaacag
     4381 aagaagcata tattgaagta tttgacacac cagcatgcta agtcgagaag tgtaactcgg
     4441 caaataaaaa aagccggatc gctccgactg atgtaataaa tccgacaagt ttattatatc
     4501 acataaaagg agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca
     4561 agatgtaatg cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc
     4621 tctttaatag atattaagtc gccgattatt acggatatgc cgaagacacc aaagcatggc
     4681 aataaaacgg aagacgcgat cattcagatg atggatatag aagcagagag agacgcgatt
     4741 ttagcggctt tgatggctct cagtctgatt agccgtcaga tactctacta cagcttctgt
     4801 gacgtaaata agcactctaa ttatgaaata ggtcaattga tacgaggata tggagagaag
     4861 aacgtagaaa aattgaaatc tatcgcattg atcgaatttg cagaagcata caaaaaaggt
     4921 gtgttagttc agtatcgttg attttgtagg gtttttgtag ggatagtgta gggtttttga
     4981 gtgttttaac gtgatattat ggtagtgtcg aaagattagt gataggtctg agacaaaata
     5041 ataataaaag gaacatcgtt ttattattgt ttcacaatta agtttcgata gacagcaacg
     5101 gaaatattaa gaataaggat gtgaatttca actccttcta aattgttctt attatctatc
     5161 attcgttgct gtctattgtc attatgtcac tgtggcggaa aggtaacgct aataacttaa
     5221 atcagtggta atgagcacct gagcttagtt tatgcagcta ggcaagagac cgtcgagggt
     5281 aaagggcaag aggcatcttg aaataagtcc cctaaagata gacaattcta gaggtgagag
     5341 tttttatgat ggaaagtctg taaaatatcg gaaaatggtg ttgcaaggtt cgattccttg
     5401 ccagtgactt tagcaaccga gggggggtat gaactcgtgt ggtacgagcc ctagggagga
     5461 acaggataac cgcctgtgtg taggttgcta ttacataatt ggttaggtta gattgagatt
     5521 tgggattcgg tacaaatgaa tcgtcaaatg tctcaagcac aggatcggaa acgtccctgc
     5581 ctgtgcatta catattagat cattctttga gtggtctttt tattttgcat gaggagtggt
     5641 gcgagaatgg attcattaga ctttttacaa aacaatgtag tgaaagagtt gaaagaaaaa
     5701 ggattcgaat tagaagatat tcaaaagatg acttttgagg atatagccaa caatttatac
     5761 tatcctaagt tccgccttga agtagacagt cagtacggtg aatctgtact ttatgcaagc
     5821 gagaaagttg tgagcgaaaa ggattcattt atgtacactt tgcctaacgg tactctgatt
     5881 attactaaac ctgcactatc aaaatacatc gagcgatttg ttgtagagtc tcgtaagcaa
     5941 cacagcgaat ttgggaaacc cttttatata aaaaaacgca acgaaccttg ggatcaagac
     6001 gaagaatgtc ttgagtatga acgaagcata ttgaagaagc gtgatgaaaa accgggacct
     6061 ccaataagaa aggactaaca ccatgagaaa ctactggtat gtatcactaa cgaataggta
     6121 tccacaacca aacgcagatg atccaattag agttgtccaa tcagtccaaa ttaaaaagaa
     6181 gtattccatt gttgaaatga caagagaagc tacaccgaaa gagatcgata agtacaatct
     6241 tcgttactgc ggccatggat attggaaaga cgaatatatt caacaaaaca ttgagaggta
     6301 catcaaatga atgataacta tgattacatc aagttgattg agaagataag agcagagaaa
     6361 gatatggatg aactagctac cttattcatg aacatcatta gtttggtggg gttaaagatg
     6421 gatgaagtag cagcactaaa ttatttcatc gctgagcaaa caattagagc agagcacaat
     6481 gctgagttct tgaaagatag actagatcta gatgtaaaag gattaggcgt tgaagggata
     6541 ttcaaagtgc aagaagcctt agtgaatgtt tatgtcgaaa agatgcaata gcattaacaa
     6601 gtaattattg gatgttttca ggagggaaac tatgaaatta aaagattata ttagggaagg
     6661 gtataacatt gtaactacac caaatctagc ttataaaatc caagaagatt atcccgatgc
     6721 tttagtgttt actgatagag ctttagatgc cattcctata gggaagttat tagttgatcg
     6781 ttattatagc aataatccag ctgttcttag tgctaagcca cttcaaaacg cttatgctat
     6841 tgaacgcttt acttgcgagt ttactggata tgagacggta gtaccaatga caggtgagac
     6901 aggaagaaaa gtaattcaac agatcaaaaa gaggattgct gaattgacgt tagatgatgg
     6961 aagtaatacg aaccttctag aaatcaaact aagagatact gactcagtac cagaagttta
     7021 ttataaaggt caacgaatgg atgagatgcc gataggtctt gtcgatatta cttatcattg
     7081 gcatacggaa gaatatgaat gccaagtacc aaaaggagct aacgacatca ctattgaata
     7141 ttatgattca cataataaca agtacttaga tagaaaaatc attgggcaca agagagatat
     7201 gtaaatgatt gatgtttcaa ctaaacaagc aagagctagg ttctatggat caactgagtg
     7261 gaggaaactt agaagtcaat gcttagagcg agatcattat gagtgccaat ggtgtaaagc
     7321 agagggtaga ctaacaactc aacatgattc aattcttgaa gtggatcata taaaggagct
     7381 ggagtatcat ccgaatttag ctctagatat aaacaaccta agaaccttat gcaaagattg
     7441 ccataacaaa aggcacaacc gtatgaaata tcgtggccaa caaaagaaaa gaaagtggga
     7501 tgatgaatgg tggtaagaaa aacagcaaac agagatgatg tcaaacgagc agaagcaatc
     7561 gctatggttg ataaggctat tgataagttg gttctattta agagtcagct cattgaagga
     7621 acaacaactg tagaggatat atctatcaac aatagactaa taggtaacgc taatgaagta
     7681 acagggacat ttaatattaa tgtagacatt gattatacct tgagtccatt tgcacatatt
     7741 gtagaaagca acgagcaaga cggctgataa atcagtataa agcgaagata tatagagtga
     7801 aatacccccg gtcaaaagat tttgagaaga aataaaattt ttgggaaccg gtggatgggg
     7861 ttaattctgc aaatgcaacg tatttatttt acccctcccc cctccgccgc ccctttagta
     7921 aatatagaag gaaggtgatt ggtttgaatt ctaagttggt tggcacgaga atgaaacaaa
     7981 aaagaattga aaaaggattg acacaggctg atgtagcaac gaaaagtggt atttctagta
     8041 ggtattatgg aagtattgaa aatgggaaaa attcccctag tatcgaaacg ttaaactcaa
     8101 tttccatcgt cattggatgc agtttacatt tcttactcaa tgatcgcatg gacgacatgg
     8161 aaaaacgcgt agagctagaa aagaaacgtc tgcagaagat attcgataaa attcctcatg
     8221 acaaacttaa tttagttgaa gggctgatca ttcaggcagc aaggttacgt gtcttgttgg
     8281 atgataactg gaaggacatc attgaaaacg gggagtacga gaaatttaaa caaagcgaga
     8341 accaaattgc ttatgataga aagcgtccta tagttgaaaa ttatgataat cgagataaaa
     8401 cttatcaagc catcgtcaaa caactcaccg atttattacc gcaaggcaca agagcggaca
     8461 agaaaagtaa gttactcggt aggaaataaa aatgcttttt aaccagcacg ttgatcaata
     8521 tattcgtaaa tggaaatctg gtgagttaat ccttaacgaa aaaaggatac aacttttaaa
     8581 actaatagaa aaagaaatat tacctcgcga cgacatttat tacttcgacg aggagcaaat
     8641 aaataattat atcgagttta gtcaagcatg gtattttgag ttagatgaat gggagaaatt
     8701 tatttctgca ttcatttttt tgttttatag agaagatgac gaagttgttt ttgatgaatt
     8761 tgttattaat atggggcgtg gcgggggtaa aaatggcttt atttcaacat tagcaaatta
     8821 ttttgtcagt tcgcttcacg gggttgatta ctatgatgtt tcaattgtag ctaatagtga
     8881 gaaacaagca aaaagaagtt ttcaagaatg ctatcgagta atcaataaaa aaggaaatga
     8941 agatttaaaa gaagagtttg aagcgtataa aagcagtcta accggtttag agacacagtc
     9001 tgtttttgag tacaaaacta gtaatgcaag ctctcaagat ggcggtcgag aaggtgcagt
     9061 aatatacgac gaataccacg aaatggaaaa cacagatatt gtagatgtgt tttctggtgg
     9121 actggggaaa gttgatcacg gacggcaatt tttcattggt actaaggggt ttgttcgtga
     9181 aggttacttt gatattaaat atagagagtg tgaagatatt ttaaatggtt tgtcagagtt
     9241 caagggtgta tttccgtaca tatgcgaact ggatgccatt gaagaaatgg atgaccccaa
     9301 aaattggccg aaagcaaacc cggcactgca accaccgttg aataaacgtg ggaaacgtct
     9361 gtttaacgaa gtcatgaaaa agtacaaaaa gttagctaaa gaaccttctg gacgctcggc
     9421 atttgttact aaacgaatga attttcttga agacaatatg gaaaactctg ttgcttcaag
     9481 agaagaaata atggcaacaa atcgtccatt ttttgaacta gatacggtac cgattggatc
     9541 tctcgatttc ggtagtgttc gagattttgc tacttgcggt ttgctattta aaaatcgaaa
     9601 gaaatcggaa tacgcattta aatcatttac gtttgcaatg aaaaattttt gcgacgttca
     9661 ttatggatac tcgttgaaag agcaattggt tggcagtgaa aaacgggcac caataaaaac
     9721 ttgggaaaaa atggggctga tgaaagttat tgacgagcca tctttaaatc ctaagtatat
     9781 tgttgattgg tttattcaag ctagagaaga atatggagtg agagtaattg tcgtggataa
     9841 ctataaggca gatattttag ggcctatgct tgaaaaagaa ggatttgaag tagttcgact
     9901 aaaaagacca tctagtttac atccattatt agctcctcgt atagaagatg gatttgcaaa
     9961 tcacaaattt atttttggag ataatccgtt aatgcgttgg tttactaata atgtgtacgt
    10021 aaaggaaacg caagcaggca aacaattctt aaaaaaagag gaagtcaagc gtaaaactga
    10081 tgggttccaa gcatttgtac acgcattgta caaagctaca gaattagatg aacagattga
    10141 ttacgatgat gcttttgata tgttggatga gattgaattt taaggtatac ttttctttac
    10201 aaaggggtgc tttcattagg ataaaaggaa aaaacatatc ttgggtattt tacatgttgg
    10261 ggattttagc ttttacattt gtggcaataa aactatcaga aattattatt ccaaatgaca
    10321 gtgtagtttc aaagaatttg gttttagaaa gagctaaact aattatttca attttaggac
    10381 cagcattaac actaattgtt tttgttcgaa caattcaaac tcaattagaa tcttcaaaaa
    10441 acttaattaa agataacgtc agtaaagatt tttataattt gctaaattta ttcaaaagta
    10501 tacaggataa aatgaattct gaattattta gtacaattga aaaaagaatt aaagtaccta
    10561 taaataatat aaatacaaat ttatatgtta gagaaaataa agaaactact aaagaagtac
    10621 aggaggattt aaaagaatat agtgcatcat tgaataaagc tttaagcgaa agcgttaaaa
    10681 aaatcaatga aatatttgag gagaactaca aagacatagg tagttatcta aaagtattcc
    10741 ataggattct taaaatacta aataacaggt atgaatcaaa agatatttcg catgaagaat
    10801 acaaaatgta tgttggcata ttacgtagtc agattgaaga ttacgaattt tatataattc
    10861 ttataaattc tttgtttact cttcgcggaa cgggtatggg gattgagtta attgaaacat
    10921 cattctttgg tgatgaacta gatattgatt taaaacagca tttcgaacat ccagagtttt
    10981 taactgaaat cattaaggat aattttgttg aaaagaaatc gtatcgtgaa tcaataaaaa
    11041 caagaaaaaa aataggaaac tatttgaaaa tagaaagata aatgataact tattatctct
    11101 tttgagaata agatacacgt tacaagatgg aaataaaatt attataagta atggagtata
    11161 gtcctagaaa tagggctttt tattttacct tgaaaggtgg tgaaaatatg tgagtttatt
    11221 tgatatcttt aagcaatcaa taaagaacga agatccatcg gattggattc ctgactttgt
    11281 gtttggtgat gaagaatctg ctagagcata tttgaaaatc atggctaaaa gtacagttct
    11341 tgattttgtt gcaagaacta tgtcaacgtt agaagttaag tttaaaaata aagatgggac
    11401 atcagattgg gattacattc taaatgtccg acctaattct gatatgtcag cagctacatt
    11461 ttggcaaaaa tttttttatc ggctcttaga tgacaacgaa gtgttagtga tatttacaga
    11521 tgataatcaa ttgctgattg ctgatgattt tagtcgaaca gagtatgccg tgtatgatga
    11581 tgtttttaca aatgtgatgg taaaaaattt tgtctttgaa cgttcatggg ccatgtctga
    11641 cgtcatttat atggaatata ataacgaaga attagaaagg tttacacgag gtttatttga
    11701 agattacgcc gagttgttcg gacggattct agaagttgca atgcgcaata accaaattcg
    11761 tgggtctgtc tcaattgatt caacaggaac ggcaaatgat gaaaaaggaa aagatggtaa
    11821 gactagatct caaagattac aagaatatat tgataagatt tggaactctt ttaaaactaa
    11881 ctcagttgca attgttccca aagtgaaagg ctttaagtat gaggaataca ccaataaaca
    11941 aggcgtatcg aatcaatcac tagatgaact gaataagatg aaatcgtctt taattgccga
    12001 tgttgccaac gccataggag tacctacggc gcttatttat ggcgaaaaag ctgaactaga
    12061 ttcaaatatt aaagcttttc gtaaattgtg tattgctcca ctagttaaaa aacttcagga
    12121 cgaattggtg gcaaaaataa ttagtcgaca agagtacaaa aacggggtac gtatagaagt
    12181 taaaaaagtt ctccaaaaag atctattaga gtatgctact caaattgaca aaataacatc
    12241 tggtggtgcg ttttatattg acgaagttcg tgatgaagtt gattatgatg agttgccaaa
    12301 taatgaagga aaaattcgta ttcgtacgaa aaactatgaa gaaactgtga agggaggtga
    12361 gaacgacaat gccgaaagct aaaaaagtac catttcagtt tactaacgaa attcagaatg
    12421 gtaagcacat tctcaccttg agtggaaatg tacagaaaaa atattggtgt gatgatgatg
    12481 tcataaacgc taaagatatt cgtgactctc tagatactgt aaccgatgat attgtaatca
    12541 agttgaacag cccaggcggt gatgtgtttg aagggattga gatttacaat tatttaaaag
    12601 atcacccatc aaatattact gtagaagtta cagggcttgc cgcaagcgca gcaacgttta
    12661 ttgtcgctgg tgccaatgat gtgattatga atgttgggac ttctttgatg attcatgaag
    12721 cttctacatt tgcttggggg aataaacagg acatccaaaa aacgttaaac gcattggaaa
    12781 cgatcgatga ttctatctta gctatttatt cagataagac tggccaatct gttgataagt
    12841 tgcgtgaatg gatgaatgaa gaaaaatggt ttacagcaga tgaagctgtc aaatatggat
    12901 ttgccaattc tgtgaagcgt gaagaaccac aaaacgaacc tcaaaatatt gcagcgatga
    12961 ttcaagatgc tgttgccgtt gcaatagcga atttaaagga acctgttact aataaaatcg
    13021 aacaagaatc aaaacaaaaa tcattaatcg cacgattgcg aaaaggagaa taaagatatg
    13081 ttaaaaatta cagataaaac tgcagatgca aaaaaggcat ttaatgctat ttcttcgaaa
    13141 gaagatgcaa cacccgaaca aattaatgct gctctagaag aatatgtaac tgccattgca
    13201 gaagatgccg gcaaacaggt gcgtgctgaa tatgaagaat tgaaaaatgt gacggacaac
    13261 cgtgtattag aggctcgtgg aattcctaca ctaacagcag aagaaacaaa gttttataat
    13321 gaagccgtaa aatctggagg tttcgattct gacgtagttt ggccagaaac gattttggaa
    13381 cgtgtttttg aaaatcttca aaacgatcat cctattttag gcctcattaa ctttacacca
    13441 actgtaggtc gtgtcaaagt aattagagca cgtcgtaaag gtgttgctgt ttttggacca
    13501 ttgcataaag accttgaagg ccaactagat gctgaatttg gtgcaactga atttgttcaa
    13561 cttgcgttga ccgcattttt cttaatttct aatgatacct tagatttagg accacgatgg
    13621 attgaccgtt ttatcaatct ttctttaagt gaagcagtac gtgatgtttg ggctaaaaaa
    13681 attattacag gttctggtaa agatgagcca attggattgc ttaaagatct agatggtgcg
    13741 gtcacaagtg gtgtatatcc agataaagct agcgcaggga cattaacttt tgcgaaagag
    13801 aagattgttc ttgaacttgc aggggtgatg aaaaaattat ctaaatacac atacaaagta
    13861 gatggtgacg atgaaggtga agtgaaatac cgttctgttg ctggacgagt gtatctaatt
    13921 gttaatcctg ttgattactg ggatatcgta gctagagtta catttgcaaa tttaaacaat
    13981 gtgtatggga ctacgatgcc atttatcgct gttgatcata tcatcgaatc tgtagacgta
    14041 ccagaaagta aatgcattgc atacgttgat aaagagtatg aagctactca atcaagagct
    14101 gaaaaagtct atgtatataa agaaactttt gcaatgaaac gcgcaacttt gtatgctgtc
    14161 gacatgcttg gtaatggcta tccaataaat aatgatgcag cacaagtata tgacttggac
    14221 tttacagcgg gggagtaacg tcccctgcaa ttggtgagat aacaccgacg actgatgggg
    14281 cagtcattaa tctttcgtaa atggggttag agtatgaaca ctgaagaatt tataaaagaa
    14341 tataaggccc gatttcgtat tttccatact tccgaagatg aggatatcgg gaaacagtta
    14401 gagagcgggt ttgccgatat aaaatctcta atcggcagtt ttgatccttc cacttatgag
    14461 aaaggaaaag aattagttta tgaacgtact cggtatctac ggaacgaatc actagagtac
    14521 ttttacgaca attttcaaac aatgattatg gatgcatcaa ttgatctggc aggtgctgga
    14581 tatgccaatt aaatcaaatt ataagaagcc taaaattgta gctggtgatc taaatacgcc
    14641 agttactttt tttgagtttc aacctaataa gggtccagaa cctggcgatg aagaaaaaaa
    14701 ggaactgtat ttttgtacag cactagttta caacccatca atgaaagatc gtgacattct
    14761 tggcacaaaa gaaggcgtta cgataaaaat acgggatccc catcaatctt atattcctac
    14821 taacaaacat aaagtaagga tattagatta tcgatatgga gataaagttt gggaaataat
    14881 cgatgtctca ccggatttgg aagaaaattc aattgtaaag atagtattag aattaattac
    14941 ttaaatttgg tgataaacga tgagtaacgt aaagtttgaa ggcgttcaag aaataatcca
    15001 agctttagaa aaaaagtttg gagaaaagaa aactaagaca ttaacaaaaa aagcaatcaa
    15061 tgttggcgct gaaaaagtag aaaaacagtt gcaaacggac atgaccgttt ttaaagacaa
    15121 aggttatacg atcgctgagg ttgttcgcaa gaacgctacg tatagagatt acaaggcaga
    15181 agctgaaatc ggttggaatg gtccacatca acgatataga atcattcatt tgaatgaatg
    15241 gggatataca cgaaagggca aacaaattag accgagagga tttggtgtaa taacaaaatc
    15301 tcttaagaat tccgagctgt tgtactttga cgctgtagga gaggaggtta aaaagagttt
    15361 atgaaagata tgcttaacat catttacgag gctctttgtt tgaatgagta tattcgttca
    15421 atgacctaca acgagaatac cgagcaatat cgtattaaat tttatgagca acctgaaacc
    15481 gccgataaaa caggtccaat gatcacaatt cgtcctgtag atacacctaa cgaagcgtac
    15541 cacggaagta acaaagagct ttctgtggag catatgatac agattgacgt tgaatcgaaa
    15601 tatagagcaa catgcaagca gattcaatat gagataaaaa aagagatgaa aaaactaggc
    15661 tttggccaaa tttccggtca agggttagat gaatacttta ctgaaacaaa ccgatttgta
    15721 gacgcgcgtc gttacgaagg aaatacaaaa atttacgata ccgaatatta gaaattaaga
    15781 cacgaaaata acgtgtcttt ttttattacc caaaattagg aggaaaattt tatgacttta
    15841 gtaggattca aaaaaatgac aatcggggtc tttgacagca cagggaagat tccagcagca
    15901 aatttatatg taattgaagg tgaacaagac aaaggggcga ctgtatctgc tgaaatcagt
    15961 gggctatcaa aagaaccatc aaaagtttac ggatcgaaca tcgcttacta tgtctctcaa
    16021 aaaggaacag gcgatgtttc agctactttt ggattgttag atttaccaac agaagtaaat
    16081 gacaaaattc ttggttacaa aaccgataca aacaaaatca gcttcttggg agaagatact
    16141 gagccaccat attgtgcaat tttgatggaa tcggaggatt tgaacggcga tacagcaatg
    16201 cttgcgatgt tcaaaggaaa attcagtcgt gaatctatta atcttaacac tacgacaaat
    16261 gaagctttcg aaccagaagc agaagaatat gtattttcag cgattgccaa tgatgttgaa
    16321 ggtgatgcta aaggccaaac agtcgcaaaa tatgttggag acgaagaagc ttctattact
    16381 gctttgaaag agatggtatt tcctgcagcg gggaagtaac tagctccgtt gttggagcaa
    16441 tcactcccac tattaatggg gtaacagttg aattaagtta ggaggataac tatgccagat
    16501 acatttaaaa tctataaaaa aaatggtact gaatttacaa aagtcgcaga gggtgaaagt
    16561 ccactttcta ttactggaat tgcagcaaat actcaagttg ctaaaggaga ctatcaagct
    16621 acgcgtgtgg tcggaggtca agaatcagta aaagtagata ttcctgcatt taccactctt
    16681 ccaatttcag ttactggtgt gacgttggat aaaacgacag ctgaagttga acaaggcgga
    16741 accttaaaat taactcctac tgtaacacca gcaaatgcaa cagacaaaac aggaagctgg
    16801 agcagttcga atacagctgt agctactgtt actggaggca acgttacagt aaaaacagac
    16861 gctgaagttg gcggaactac agaagtttca tttactacta cggatggcgg taaagtagca
    16921 aaatgtacta ttacagttat tgaaaaagca gcgggctagt cttcggacta gcttttttat
    16981 ttggaaaata atggaggaat ctaaatggca aaagtaagaa ttgaattgaa agaagaatca
    17041 ggaaagaaag tctatgaaaa catagacaca acaggtaaag attatcgaaa agctttagaa
    17101 acaatcaaaa agctaaatga agatggagta atgatttgga atcgtttaga catttatttg
    17161 gattttgctg taggtatctt cagggatagc aaattgacag ctgaacaaat tcttgatggc
    17221 ttaccatctg agaaggtaat gagcacattg gacgacatcc taggagaagt aatggggatt
    17281 gaaagtaatc cagacccaga cgcaaaaaag taacacccga agaagcagaa gaaatgtact
    17341 taaacctatg cagagagtta gtgaaagcgg gatggtcact atctgacata gaagataatt
    17401 cctttgaaat gatgatgaaa attgcgtgca cttcgcctaa gaaagagaaa caaaaagaag
    17461 ttgatcttaa agacttcttg aaatccatgt agagaggagg taaacatatg gcaaatggaa
    17521 aacctttagg caatatgatt atcaaactag acttagacag ttcagccttt tcaaaaggtc
    17581 tagccggagc taaaaacgct gttaaccatc aaatgaaagc aatgaaatca cagatgcaag
    17641 taatgaactc ttcagggaat tctctcggtg ctttacaagc aaaatataat ggtttaggcg
    17701 gagttctaca agcaaatgaa aagcaagtag aattgctcac taaagcttat aaggatagtt
    17761 ttgactctaa cggaaacgct actgcttcga ctgctaaata cgctaatcaa ttgaatcaag
    17821 caacagctag atcagcaagt tacgaatctc agatgaaaac aactgttgga cagattgctc
    17881 ggatgaaagt ggaaacggaa gggtttacgg ggaaactaaa agctaattct gatgtattga
    17941 ttaattctgg gaaaaaaatt gaagcctttg gtaaaggagt ttctgggata ggatctgccc
    18001 taactgttgg agtaacagct ccaatagtgg caggcgcaac tgctgttact agtgcagcta
    18061 tttcatggga aagtgctttt gcaggtgtaa aaaagacaaa tgatgaagtt gtagactcga
    18121 atggaaacgt agtttattcc tatagcgact tagagaaagg tctgcgtaat cttgccaccc
    18181 aattacctgg tagccatcaa gaaatagcaa atgtagccga agcagccggg caattaggta
    18241 taaagactga aaacgtagtt agcttcacta aaacgatgat agacatgggt gaatcgacta
    18301 atatgtcggc tgaaactgcg gcaacttctt tagcacgatt tgctaatatt acccaaatgt
    18361 ctcaaaaaga ctttgataaa ttaggatcag tcattgttga tctaggtaac aattttgcta
    18421 caaccgaatc agaaatcact gaaatgggat tgcggttagc gggagctggt aaacagattg
    18481 gaatgagcca aggcgaaatt ctaggatttg ctactgctct atcttctgtt ggtgtcgaag
    18541 ctgaagcggg tgggtctgcc ttttcgaagg tcatgatcca gatgcaacta gctgtagaaa
    18601 aaggatttgg cgcttttgat cagctgaaac agatggcaga agaacaaggt gttccgtggg
    18661 ttaatttagt aaatgctgtg agagacggtg gaaaatcgtt aaaagcagta tcagagcaaa
    18721 tgggattcac ttcatctgat ttaaagaaaa tgtataaaga agcggacaat tcgaaaagtt
    18781 cgcttgaaaa ttttgcgaat gtggcaggtg ttacaagtga acaattccag aagttgttca
    18841 agagtgatcc ttcagaagca atcatcaagt tcattcaagg gctgaaagat tcagaaaaac
    18901 atggtacttc agcaatcaaa gttctggatg acatggatat aaaagaagtt cgactacgcg
    18961 acagtttatt gagagctgca aatgctagtg acatattcaa aggagcaatt gaaaaaggaa
    19021 acggcgcgtg gaaacaaaat acagctttaa caaatgaagc taataaaaga tacgaaacaa
    19081 ctgaatcaaa gctaaagata ttgaaaaacg aagtgattga tacagcaatc gatcttggag
    19141 gtccatttgt agacgcgttg cgtgatggtc taaatgtatc gaaaccattg ataaaaaact
    19201 tagggaatat ggcgaaagct ttctcagcta tgagtgatga tcaacaaaga aacattatca
    19261 aatgggttgg attagcagca gcagctggtc cagcgctgaa gcttttagga actggtactt
    19321 cagtaatagg taaagttact tcaactactg gaagattgac gaaaagcctt gttgatttag
    19381 cagcgaaagc agcagaaaag aaggctatgg atgcctttgc atcgtcggta acaacagctg
    19441 gtgctgcagc tgcaaagaca gcaggagctg gaggtattgg cgggttagtc cctatgatgg
    19501 gaaaaacagc tagtgctgca agtactgctg cgggcgccag cggaataggc gcaatgacaa
    19561 cgtcactagg tttgttaggt cctgctctac ttggtattgt tggcgttggt ggcgcactgg
    19621 cagttggcta tggagcatgg aaattatttg gtgaagaagc ttggaattcc tcgcaaagag
    19681 taaagcaatg gggaacagat gtcggtgagg agatagatag tactcttgac ggagtacaga
    19741 agaatattga aggtgcaaat ggtcaattcg gtcttctcaa agagggtttt actgttgatg
    19801 atgcaaataa tatggcggct aatttcgcaa ccgctggaac atctttggaa acatctttaa
    19861 cgggtagaat aaaggcatta gacgatgcac taaaagggct acctcaatca gttcaggatg
    19921 ccatgaaaga agtagctgaa tcagaagaag aaaccttagg gcaatctctt tctaaaataa
    19981 aagacaacaa tgaagaaata aaaaaaatca gagaaagagc cgcaaatcag aacagagact
    20041 tgactgtttc agaactacaa cagatacagg atctaatgaa aggaacttct caagcgtatg
    20101 tggatacgct cgatgtttct gctaaagaca gaaaaaagat actatcagcc atgaatggtg
    20161 atgttgcaca agctacagaa gaagaagcga agacgtggtt acaatcttta gcgaaacaaa
    20221 aagctgcaac taccgaaaat tatagacaaa tgcttgaaga aaaaaaggcg gctttaaagg
    20281 aaaagggagt aagcaaagaa ttcattgatg cattagaaga tgaaatgaat gattacatca
    20341 aaactacaaa tcaaggattc gatagccaaa tagctgctat aacaaataaa tatccacaat
    20401 tgattaacga agtgagttta tcaaatggtc aattatttag caaaacggca gctgctatgg
    20461 acaatactgg tcagtataca aaagtttttc tcgaaagtaa tgaaaagatt ttagcccaag
    20521 cgaagaaaac atcggatgaa atagcagata atgccgaaaa aaatgcagaa caacttggta
    20581 tggtagctga tgaggctacg catgctggat caacttggaa tggcttagtt ttagacgaaa
    20641 aaacaggaaa agtaaaaact aatgtacaag aagtagtgaa tgaagcaacg aaggatttca
    20701 ctaaatggaa tgaattgaaa cctgttgttc atgatgcgaa actaacaacg aatgcaaaag
    20761 atgtcatttc catagcggca gtacaaaatg gctattggga tgatatggaa tggaaagaaa
    20821 aagaagcatt attagaagat gagtgttcta aaaatgtata caaggcttta gaaaatgctg
    20881 gtaaatggaa tgagttagat attccttcta aagaagcaat cctaacttca aatactcctg
    20941 aagttatggg tgaaactctc atgaatttgg gattatggga tacttacaaa cttcaagtga
    21001 aagatttaga tttgaatgcg tataaagtat acgatgctat cgatggatca gaagaaaaat
    21061 taaatagttg ggctcaatta gataacccca caaaagaatt gttattagat aactcagatt
    21121 tttctacaaa actttttaat tccgaagaaa tgtttaaacg ttggaatcag ttatcatcta
    21181 atgcaaagtt tctttcggct aataatacta aatttacaga ggttctttta tcatctgaca
    21241 gaatgtggaa tcaatgggta aaccttcctg ataatgagaa aagattactt gcagataacg
    21301 aagatttagc taccactgtt ttatcttcag aagaaatata cggaagatgg actcaattac
    21361 cagataatca aaaatatatg ttagctgaca atactgactt agcaaacaaa atattcgctt
    21421 cagaagaatc atggaatgca tggaaagcaa ttccagacac agttaaaaac ttgcttggta
    21481 ataatattga tttactggct aaagtgaaag acgggactgt aagtgtagaa gaatataaca
    21541 aagttatgcc acttcttaaa actctatatg gagataacgt taatttaaaa gaggcaatca
    21601 gtggggctaa aagctctata gagaattaca acaaaaatat ttttcctagt gataaaactg
    21661 cagtaggaca cgatgaggct tcacaagctg ctaaagatgc atttgaagcg ttcaacatct
    21721 tccaaacaaa aataccagat aaaattacta aaactgtatc agctgacttt ataggtccta
    21781 tgccgaaaaa tgcgaaagga acaaacttcc atcctggcgg agcagctatg gtaaacgatc
    21841 aaaaagggcc tacttatgaa gagcttataa ccttgccaag cggagaaact tttataccta
    21901 aaggacgtaa tgtcgtttta gacttaccaa gaggttcgaa agtactgaac gccactaaaa
    21961 ctaaacgtct agtgcctaaa tatgctgatg gtataggaaa cataacgacg gtttcatcta
    22021 cgctaaactt agatgcttta attttagcta ttaacgagct aacgacggta ttgagaactc
    22081 aactgatggc aggaacgact gatgctaaag atactaaact atcagatgaa acaaaagttg
    22141 ccgatccgat tattccggac agtctttctg aaaaatcaga tcagtatcta ggaattggag
    22201 ctcaatggtt aactaatcta atgaatggtt ggaactcagc ggttcctcaa tatatgaata
    22261 gtgaaatggt ttttattaca aactatctca atgcgctaaa acttcaaaac aatcctaatt
    22321 atacgcaagg agcaacatgg aataagaact tactgaatgg atggaatagt ttaactggga
    22381 cctttattgc tacaatcaat tcattttgta atcaagcaat ggtcacgctt agaaactaca
    22441 acactcctat gtacaacaat ggccgaacgt ggcagcaaaa taatcttaat ggttggaatt
    22501 cattgtatgg atcattcata gcccgtgtaa atcagcttgg taacgattcg attaacaacc
    22561 ttcgttctaa aagtggtggt ttttacaatg cagggacatt cttgttgcaa tctttaataa
    22621 acggaatgaa ttcgttaggt aattctcttt ctactacaat gaacaatgtc gctaatacaa
    22681 tggttggtgg tataggcaaa ggagttaatg gcgtcatatc gggagttaac tatgtgctta
    22741 aggaagtcga atcaagtaag aatatcggaa actggccaat tccacaatac gctaagggaa
    22801 ctgatggaca tcctggtgga cttgctatga ttaatgatca aaaaggccta gttcacgaag
    22861 aatatgttca gatgccagat ggacgtagtt ttattgccaa aggtagagat cttcttgtta
    22921 atttgcctaa aggtgctcag gtcttaaatg cttctttaac aaagaaatta aaggaacgtt
    22981 taaatattcc acactacgaa aaaggtattg gcaatttaga tattgttgat ttgttagatg
    23041 atgaaaaaag aatgttagaa ttcttaacta gcaaagttga tttttcaggc attaatgaac
    23101 catggcttga tatgactaaa tcaggaacgt cattaatgtc caaagctgca aatacaatgc
    23161 ttcaatcaaa attaagtgaa ttcttcactc atggcaattt tgatggtgca gtaaatgcca
    23221 atggtgttta tcaatattta gttgatgtgg cacagaaagt aatgggtaag ttccccggac
    23281 ttacagtaac ttctggatat cgagcaggag atgcctacta tcacggaaaa cggcaagcta
    23341 tcgacttggc ttatccaggt atttctggag atccgagata tacagcagca gcaaattacg
    23401 cttttgaaaa attcccttca aaaattgcgt atgtcattac gaatggacgt gtacgtgacc
    23461 gcatgggatt atctggaaca aggtcaagcg gacaatggac gaattggcca gatggcgatc
    23521 acttcgatca tattcattta aacggttcga tggggtctgg cgatatattc aatggaggag
    23581 ttggaggaag tggtgttgca cgctggcgtt cttatgtgtc taaagcattg aaaatgaatg
    23641 gattaccagc tacagcagcg tatataaatg catggatgtc ccaaatccaa acggagtctg
    23701 gtggtaatga gagagcaatt ggtggcactg atgggctcgc agaaggaaat gcgacggggt
    23761 tacttcaaac aaaacccgga acttttgcag cgaatgcttt tcctggtcac ggaaatatta
    23821 tgaacggctt cgataatatg ttagcagcta tcaattacgc taagaaacgt tatggggttg
    23881 ctggcatgtt gcaagttatc gggaaaggac atggatatgc caatggcggc ttgattacca
    23941 aagacggact atatcgagca ggagaaggaa ataaaccaga aatggttatt ccattgacaa
    24001 gaaaaacaag agctatcgaa cttatgggtc aagcattagc cttcctttct ggaagcaata
    24061 aaaaagaaac gaaaagtgag attaattctg tagataatag acatatccta caagcgatag
    24121 aaaaacagtc tcgtatgaca aatgatctgc tatcattatt actctcgttt tttaaaagta
    24181 acaatcgatc agataaagaa ctagctttag atattcagaa aattttaatc aggaggacat
    24241 aggatttgag gacagtaata ttaagaaata aattgaatga agaaattgat ttgtccacag
    24301 aagattattt tgctactgaa atgtcgaatc taggttttga agttacaaaa gagcatattg
    24361 gacaatgggg gagtttcaga gaaataggag agagtattga attgtctgaa tttcaatcat
    24421 cagttatgat ttctgttcac ggatttagag aacaacaatt atatggttca ttagttgaat
    24481 ttctggctaa tggacctttt gaactagaat tttcttttga ttcagaaaca atgatcagga
    24541 aatgtagttt gaaatcatta tctaaaacag aaattgattc ccaaagctca ttgctaacag
    24601 atactttgaa tctatatttt acttcaagct ggtattctat taagcgtgaa aaattaatac
    24661 agaattcaac ttctctcaat acacgtggaa aagtttactc atttgttcga ccatatgtct
    24721 acaccaaaaa tgtatgggaa aagaaaggtg tatttaagtt cagtaataat tctgtatatt
    24781 tgacaaatag tacagagcga atgtctccgt taaaaataag gattattgga gagtgctcaa
    24841 atccatattg ggaagttatc caaaactcta aagtagtagc ttcagatggc tattttatta
    24901 atatgaaaga cacacaaata ttagaagtgt ctagtttgct agaagaccaa acggcaattt
    24961 taaaagatgt aggtggagtt gaatcttctg tttatcaaca acaagatttt actaaatcaa
    25021 acttcgtcca agcgcctcta ggagaattca gcgttgtttt tcatgttgga catgcaaatg
    25081 tagagattga actttatgag gagcgtgatc tattttgatt ttagccatta ccctctttca
    25141 ccgagatatg aatttatata acgaacgttt aatttcgact gagttcgaat ttggaacgga
    25201 tgaaattgga gaagaagtaa gtaattttat tacagataaa tatgtccatg taaaatcagg
    25261 agattttcta ctagcaaaat ttattcctag cgggaagatt gcttattttg gagtgattag
    25321 ctcgcaagaa gacaaaaaaa taaattgcaa aggattactc agtttggccg atagcgagat
    25381 accgactgta aaaatatcag gtgctaatta tgaagagcat attcgacgac tgattgaata
    25441 ctacttattg aacgatccaa caaaacaact aaaaaatatt ttggaggtaa aaacagaaag
    25501 cgttacttct cattcttatc aagctgttga cacaaataag agacgattga atgcatatat
    25561 aataaatggg ttcaaaaaat ataatgttaa atggtatgtc aaagagatta aaggtaaaaa
    25621 aatatatact ggtattcgag cagttgatga ttcaatttat attaaagata attcatctga
    25681 gtttaatgac tgggatgttt ttgtgcaatc tcctggacca gggaacgaga acaaattatt
    25741 gatcgtagat aaagcaatga aagatataga aaatcccttg gtattgtcta cgtggtattt
    25801 agatgacgaa aataatctta cgcaagattc aacgaataag aacattgcat tgcctaccgt
    25861 taatttagta aatatatatg atcaaactca agaagataga ccttcttatg aagaggtagc
    25921 taaatcggag cttaaaggca acgcctattc acatgaaatt aaagttaatg ttgtgctaaa
    25981 tgctagaaat atcaatgtag aaaatatcga aacaggaatg ttagcgacta ttgtttacaa
    26041 agagcagact tacaaatctg ttctaactgc ctggcgaata tccagcagta aaaagaatat
    26101 agaattgaca tttggtaatg tccgaagtcg ttttatggat tattttgaag ataatggggg
    26161 ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag taagcgcaga
    26221 aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta ttgatggtta
    26281 cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg caggagcagc
    26341 gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag ttccagcaaa
    26401 ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac ctggatctat
    26461 tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag aatttataac
    26521 aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac cactatgctc
    26581 agttacttct actggatcaa ctgtttcaat ctcaaagata tcggatagtt acgagctgac
    26641 tctctctaaa ggagaaattt tgtggagtgg gactgcgtta atgcatgata ctcaaactgt
    26701 ccaaccttca aaaaaaatgt ggcagacagt ttcaggtttt ttattagtat ggctcccata
    26761 cgaaaacggg caagcaattg aggatagata tgtaactacg cctttttata aagagcgtgt
    26821 aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc acaaaaagtg
    26881 gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg cagcaagcaa
    26941 tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt agttaggagg
    27001 tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca gccttatcta
    27061 cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa tgtacaactt
    27121 cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt gaaatttgtg
    27181 ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga ttcgactaat
    27241 gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca attcaaacaa
    27301 gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa cttcatcata
    27361 gacgtgttag aacgagtaga tattaattcg gagcaagcga aatactatat ttcttcattg
    27421 gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga taaacaggtc
    27481 caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa tgaatcagat
    27541 aagcaagtaa atgatcgtat tgataagaca aatgatcgta ttgatcaaac caatcagcaa
    27601 atcggcgatc tcggcaagct gaaaaagatg taca
//
LOCUS       DACGLH010000029        27220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.031, whole genome shotgun sequence.
ACCESSION   DACGLH010000029 DACGLH010000000
VERSION     DACGLH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.031"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(17..385)
                     /gene="rplL"
                     /locus_tag="IXT21_001427"
     CDS             complement(17..385)
                     /gene="rplL"
                     /locus_tag="IXT21_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ4868835.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(438..941)
                     /locus_tag="IXT21_001428"
     CDS             complement(438..941)
                     /locus_tag="IXT21_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ4868836.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(1351..2040)
                     /gene="rplA"
                     /locus_tag="IXT21_001429"
     CDS             complement(1351..2040)
                     /gene="rplA"
                     /locus_tag="IXT21_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ4868837.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2144..2566)
                     /gene="rplK"
                     /locus_tag="IXT21_001430"
     CDS             complement(2144..2566)
                     /gene="rplK"
                     /locus_tag="IXT21_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ4868838.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2909..3577
                     /gene="sdaAB"
                     /locus_tag="IXT21_001431"
     CDS             2909..3577
                     /gene="sdaAB"
                     /locus_tag="IXT21_001431"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ4868839.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3590..4462
                     /gene="sdaAA"
                     /locus_tag="IXT21_001432"
     CDS             3590..4462
                     /gene="sdaAA"
                     /locus_tag="IXT21_001432"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ4868840.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4553..5572)
                     /locus_tag="IXT21_001433"
     CDS             complement(4553..5572)
                     /locus_tag="IXT21_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ4868841.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(5745..6632)
                     /locus_tag="IXT21_001434"
     CDS             complement(5745..6632)
                     /locus_tag="IXT21_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4868842.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(6709..7254)
                     /gene="nusG"
                     /locus_tag="IXT21_001435"
     CDS             complement(6709..7254)
                     /gene="nusG"
                     /locus_tag="IXT21_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ4868843.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7355..7525)
                     /gene="secE"
                     /locus_tag="IXT21_001436"
     CDS             complement(7355..7525)
                     /gene="secE"
                     /locus_tag="IXT21_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ4868844.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7549..7701)
                     /gene="rpmG"
                     /locus_tag="IXT21_001437"
     CDS             complement(7549..7701)
                     /gene="rpmG"
                     /locus_tag="IXT21_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ4868845.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7863..8507
                     /locus_tag="IXT21_001438"
     CDS             7863..8507
                     /locus_tag="IXT21_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4868846.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8563..9066)
                     /locus_tag="IXT21_001439"
     CDS             complement(8563..9066)
                     /locus_tag="IXT21_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ4868847.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9079..9125)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9663..10628
                     /locus_tag="IXT21_001440"
     CDS             9663..10628
                     /locus_tag="IXT21_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ4868848.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10727..11899)
                     /locus_tag="IXT21_001441"
     CDS             complement(10727..11899)
                     /locus_tag="IXT21_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868849.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11886..13031)
                     /locus_tag="IXT21_001442"
     CDS             complement(11886..13031)
                     /locus_tag="IXT21_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868850.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(13045..13977)
                     /locus_tag="IXT21_001443"
     CDS             complement(13045..13977)
                     /locus_tag="IXT21_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868851.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            14094..15173
                     /locus_tag="IXT21_001444"
     CDS             14094..15173
                     /locus_tag="IXT21_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4868852.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            15170..15799
                     /locus_tag="IXT21_001445"
     CDS             15170..15799
                     /locus_tag="IXT21_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4868853.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(15909..16136)
                     /locus_tag="IXT21_001446"
     CDS             complement(15909..16136)
                     /locus_tag="IXT21_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4868854.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(16239..18827)
                     /locus_tag="IXT21_001447"
     CDS             complement(16239..18827)
                     /locus_tag="IXT21_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ4868855.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(18847..19506)
                     /locus_tag="IXT21_001448"
     CDS             complement(18847..19506)
                     /locus_tag="IXT21_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4868856.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(19555..20058)
                     /gene="trmL"
                     /locus_tag="IXT21_001449"
     CDS             complement(19555..20058)
                     /gene="trmL"
                     /locus_tag="IXT21_001449"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ4868857.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(20279..21145)
                     /locus_tag="IXT21_001450"
     CDS             complement(20279..21145)
                     /locus_tag="IXT21_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4868858.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            21254..21895
                     /locus_tag="IXT21_001451"
     CDS             21254..21895
                     /locus_tag="IXT21_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ4868859.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(21957..23318)
                     /gene="mgtE"
                     /locus_tag="IXT21_001452"
     CDS             complement(21957..23318)
                     /gene="mgtE"
                     /locus_tag="IXT21_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ4868860.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(23336..24235)
                     /locus_tag="IXT21_001453"
     CDS             complement(23336..24235)
                     /locus_tag="IXT21_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ4868861.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(24237..25034)
                     /locus_tag="IXT21_001454"
     CDS             complement(24237..25034)
                     /locus_tag="IXT21_001454"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ4868862.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(25012..25716)
                     /locus_tag="IXT21_001455"
     CDS             complement(25012..25716)
                     /locus_tag="IXT21_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ4868863.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            25847..26422
                     /locus_tag="IXT21_001456"
     CDS             25847..26422
                     /locus_tag="IXT21_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ4868864.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            26540..27217
                     /locus_tag="IXT21_001457"
     CDS             26540..27217
                     /locus_tag="IXT21_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ4868865.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         7927 a         5744 c         4904 g         8645 t
ORIGIN      
        1 ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca agtttagctt
       61 tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga gcgccatcaa
      121 ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct ttgataactt
      181 taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag
      241 cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg ccaaattctt
      301 cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt aactcagcaa
      361 cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc ttttttattt
      421 aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac tgctttgaca
      481 gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga tagaagtcct
      541 tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac tttaccttcg
      601 atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc catgattttc
      661 gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt gaatacttcg
      721 tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt tttgataact
      781 ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc atcaactgtt
      841 aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc gcttacttca
      901 tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt tcacctcctg
      961 aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag ttactttgct
     1021 caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc gctttgctcg
     1081 aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc acttcgttca
     1141 tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta ttgaatatat
     1201 tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct tcggtacaag
     1261 ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga attatttttc
     1321 agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa catggatacc
     1381 aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag ctgctgatgg
     1441 tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt tttcatcgtc
     1501 aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac ggtaagttac
     1561 tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg taccagtttt
     1621 agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta cagtagccat
     1681 catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac
     1741 catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg ctttgtctcc
     1801 tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata caacagcacc
     1861 acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt
     1921 tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg caacaggata
     1981 aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct ttttagccat
     2041 tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc tatatctttc
     2101 aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct acagtgatcc
     2161 ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta
     2221 ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact ttcgcaactt
     2281 tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc aatagaacag
     2341 cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca gaaataacaa
     2401 ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt gtgaatccca
     2461 tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga gttgctttac
     2521 ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga catacctcct
     2581 taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt tatctgatct
     2641 gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat agcaaattta
     2701 tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa acgctaaatt
     2761 tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac acttgcgcta
     2821 gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt gtacaaagat
     2881 tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa gtgtctttga
     2941 tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag ctgctagaat
     3001 cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata tttatctata
     3061 tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag ttggcggcct
     3121 gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg catatgaagc
     3181 cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa
     3241 gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga tcggcggtgg
     3301 caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg gtactcctac
     3361 cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta gtatactttc
     3421 tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc
     3481 cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa gactcaaaca
     3541 gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa tgtttttatc
     3601 gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat tgatgattgc
     3661 agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca tggaaaaaaa
     3721 cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct ccgtcacagg
     3781 aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag actttttaag
     3841 tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa
     3901 aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct
     3961 gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat tcttatttac
     4021 cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg cagaaggggg
     4081 atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa
     4141 aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa atatgatggg
     4201 actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt
     4261 aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg aaagtgtcat
     4321 tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt
     4381 caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac ttgcggaaga
     4441 gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga gattagttat
     4501 cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt tattaatcca
     4561 tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg
     4621 acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca cctagattca
     4681 acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc agagctttga
     4741 agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt ttgttgacta
     4801 cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctaat tgacgtccac
     4861 catgattgga tacccagata ccatctgccc ctgcagagat agcgattact gcatcttctg
     4921 gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt gttttgatgt
     4981 catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct tttccttcgc
     5041 cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt aatggaaatt
     5101 ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct gccgttaaaa
     5161 tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg aatctgtcgt
     5221 ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt
     5281 tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca gctactcctt
     5341 tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga
     5401 taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct gagtcgattc
     5461 cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt tctttcatcg
     5521 tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt
     5581 ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct tcattacttg
     5641 tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa tcatactaaa
     5701 cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc tttccaaata
     5761 aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg agaggcattt
     5821 tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa cgcatagacc
     5881 gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc tagttgacca
     5941 gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg gtaatcttga
     6001 tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga ctgcgcacta
     6061 tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac ttgtggcaat
     6121 gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc catggaatgc
     6181 cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag acaagttttg
     6241 atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac ttggaccatc
     6301 ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc tgttcctact
     6361 gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa catcccttta
     6421 aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct
     6481 atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt atttatcttc
     6541 gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca
     6601 cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat atagaaaaaa
     6661 gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt aatcgatttt
     6721 atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat cgatgttgac
     6781 tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc
     6841 gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt gtcgcaggct
     6901 cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg ctggtttgct
     6961 tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga caacatacca
     7021 tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt tatgaacgat
     7081 ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa cgacacggaa
     7141 aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt ttactttgtt
     7201 ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt ccattgaagg
     7261 ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc
     7321 attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag ccaaataagc
     7381 tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa gtttcgatga
     7441 ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt ttgatttcat
     7501 cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt atttcgtttc
     7561 tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca aacgttcgcc
     7621 gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg aacaagcgag
     7681 tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga cttatacttc
     7741 ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc tattgttttg
     7801 gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt ggaggtatag
     7861 cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt acagagacta
     7921 gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt gtgccgatct
     7981 tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc tatcgtcata
     8041 aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac gctacaaagt
     8101 tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag cttccttata
     8161 ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt acgttgctta
     8221 atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca attgcttcca
     8281 caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac agcagcattg
     8341 ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc
     8401 taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta gagaaaaaga
     8461 attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa
     8521 aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac gcttttcaaa
     8581 atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga cgataaactc
     8641 acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg gaactaacat
     8701 ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt
     8761 tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca atgtttggcc
     8821 gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga cgatgacccc
     8881 gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca aactttctga
     8941 aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta acgctagata
     9001 aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc ttttcgtttt
     9061 ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg atgaaccgcg
     9121 gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc cgtcttatct
     9181 gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg
     9241 tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag taggatctgc
     9301 aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat ttgagcaacc
     9361 tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc cgaaccttgg
     9421 aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt gtttttcggt
     9481 cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt aattttgtaa
     9541 cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc ttgctaatta
     9601 ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag caggaggaaa
     9661 tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga aatggctata
     9721 aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat acacttcatt
     9781 atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa gaaacattga
     9841 ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg atcatcgtag
     9901 gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa gtagaattcc
     9961 cagttgctta tattccagca ggttctggaa atgattttgc tcgaggggca ggccttccaa
    10021 aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa aaagtccaca
    10081 taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat aatttcggta
    10141 taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa aagcgattga
    10201 acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct ttattcactc
    10261 aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt tcaaacgcat
    10321 ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt gttccaactg
    10381 ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat ttctttaaaa
    10441 tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa tatttccatc
    10501 actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt caagaagatg
    10561 gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa cagttgtttt
    10621 ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta ttcggaaaaa
    10681 aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa ttctttgccc
    10741 aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc attgaaaaca
    10801 ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca aatgtttgat
    10861 tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat gttggtgtga
    10921 atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac accccaccga
    10981 taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct gagtttttta
    11041 ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag aaagtattta
    11101 aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga accattagaa
    11161 gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt actttgcgat
    11221 tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct ttgcgattaa
    11281 acgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca taagacaaga
    11341 aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc gcacttagct
    11401 caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca tcgattgttt
    11461 tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa
    11521 ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcgccatc tttcctactt
    11581 cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga ttaaaataaa
    11641 gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt tccttgtcat
    11701 tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc aaggcgattt
    11761 tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag aatgtggcaa
    11821 caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc aatgtccaaa
    11881 tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc taacgcaaaa
    11941 aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa atcagtgtag
    12001 aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg accaacaatc
    12061 ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc agcaaaaaag
    12121 ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt catttttggt
    12181 atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag agcggtaaca
    12241 atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg aaagacagct
    12301 aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt agcgattgaa
    12361 actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga ttgatttgct
    12421 tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc cattccaatc
    12481 agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac ttcttgaaaa
    12541 gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc cacttctgcc
    12601 actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt tttcatcaac
    12661 gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc aatgagataa
    12721 aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga tagttcctgt
    12781 acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt aaaattttcc
    12841 gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc aagattgcca
    12901 ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat tggaaaggcc
    12961 aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata tttataaaaa
    13021 tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa
    13081 gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct tttcttcttg
    13141 taagattgta agaatcggca acaatactgt atttagttca gtagttgtta cttggagctg
    13201 acctttttta taagcgattc catcagaagc tattgttcgc tgaatcgctg acaacacatt
    13261 ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag
    13321 agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca
    13381 aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt
    13441 cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg
    13501 ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg
    13561 tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa
    13621 ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca
    13681 aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac
    13741 gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt
    13801 aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc
    13861 actgccgttt ggacctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg
    13921 attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat
    13981 gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact
    14041 agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata
    14101 aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa
    14161 gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg
    14221 tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat
    14281 gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg
    14341 aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta
    14401 atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac
    14461 gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc
    14521 attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa
    14581 cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca
    14641 atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc
    14701 aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg
    14761 atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct
    14821 tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag
    14881 gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca
    14941 gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt
    15001 atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg
    15061 gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag
    15121 aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt
    15181 agtcattatt gatgatgatc catttgtgac gaaatcgtta caaaccattt tagaaagcac
    15241 gaaagagatc cgtgtgttag gcattggtca ttgtgccaag gatgcgctag tcttatacga
    15301 aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa cgggcatcga
    15361 tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt tgacgacatt
    15421 caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt atttaatcaa
    15481 acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac aggccgtatt
    15541 tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga cgattgatag
    15601 tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag tagctgccgg
    15661 gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg ttcgcaatta
    15721 catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg ctatctattt
    15781 ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat gctccttcgc
    15841 atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt tcctcacgat
    15901 tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg cgtgttaggc
    15961 ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg atcgtctcgt
    16021 tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa ctttttaaag
    16081 tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt agccataaag
    16141 taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa agaaaacgct
    16201 aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt tgccggtata
    16261 aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg gttatcgatc
    16321 acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc tttcttttct
    16381 tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg gtttttcgtt
    16441 tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg tggctctttt
    16501 gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata agctgctagt
    16561 ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt caacgtagtc
    16621 atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc ccctaacaaa
    16681 atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg aagcatccgc
    16741 tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg tgatttatgg
    16801 attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata agtgacctca
    16861 tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt tgccaagatg
    16921 atacccgtga tttctcccat atctccattg aatacattca attcgggagt attgactaat
    16981 tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc cttttttcca
    17041 ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat ccctgcaggt
    17101 ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt atttttcgct
    17161 ttttccacaa ttttagagat caagggttca atttgataag catcacttcg gaaataggaa
    17221 cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat ctcatgggca
    17281 agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc cattttggga
    17341 atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga gggaagctga
    17401 tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa taatgtgtta
    17461 gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc ttctaattct
    17521 ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg atggatcgta
    17581 ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt tggtgctgca
    17641 agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag tccgttcaac
    17701 tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc tggtcctcct
    17761 gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg cgaaggtcca
    17821 tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc tttttcagga
    17881 taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat cccccattca
    17941 gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt tccttcttca
    18001 acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg gcgacttgtt
    18061 tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt atctcctgtt
    18121 tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct cggtgaatct
    18181 gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat tccttctatg
    18241 tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt cttataggcc
    18301 tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc catgatcact
    18361 tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt tggcttcgtc
    18421 aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat agcatcttct
    18481 cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa tttttcgctg
    18541 gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata tctttcgagt
    18601 ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg ataagtctca
    18661 ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg gtaatcagct
    18721 gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt ttgaaaaaaa
    18781 atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg aagccgcctc
    18841 cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg tttggttcca
    18901 tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa tcgtcaaact
    18961 actattgaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg tcatccattg
    19021 aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat ctttatgtcg
    19081 ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa tcgtttctcc
    19141 gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca gattatccgg
    19201 atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc ctagttctct
    19261 tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga taccttctgc
    19321 tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa catattttac
    19381 ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag aatcgccatt
    19441 gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg taaaatatag
    19501 ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa ctgtctaatc
    19561 cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt gctggcgaag
    19621 tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga catgctcatc
    19681 attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt ctggcaaacc
    19741 ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat tttcgtcact
    19801 atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta aaaatgttaa
    19861 aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat ccaaccctgc
    19921 ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat gaagcggagt
    19981 attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta atggttcaaa
    20041 taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt ctattttctg
    20101 acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac tgcgagtcgt
    20161 tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat aaaaaccaat
    20221 gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc tctactcatt
    20281 aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg tcaattgtaa
    20341 tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc tgccattcta
    20401 aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat tcataggtta
    20461 cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca cgttcattgg
    20521 aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc tttaaataat
    20581 tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt tggaattctt
    20641 ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca gcaaacggca
    20701 aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta gctgcgtaga
    20761 ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat tttgaaagtt
    20821 cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct tcaggtaaat
    20881 gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt ctacgccttg
    20941 gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa taaagtgttc
    21001 cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta tcagactttt
    21061 ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc aaaaactggc
    21121 gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc ttaccgttag
    21181 atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag tgaaagataa
    21241 aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta ttcctctagc
    21301 aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg gtggcacaac
    21361 cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag atatccccgt
    21421 aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg aattgaaaat
    21481 catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag tatttggttg
    21541 tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg aaacagcaga
    21601 agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc ggcaattcga
    21661 agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg gcggtcctgc
    21721 tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct atacagatgg
    21781 ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac atgtagctaa
    21841 agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt tataatctta
    21901 taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt gtttttttac
    21961 ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact cgtcaaatcg
    22021 ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag acgatccatt
    22081 agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag catagccatc
    22141 cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag aaaaatcgtg
    22201 atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac aagtcgaaca
    22261 aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc tagcgactgg
    22321 gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa aatactcgct
    22381 tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc taaaaacaac
    22441 agagtgatca gccaaggcaa acgtcgagac gctgccttta ctggattttc attaatttct
    22501 tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc aataacgtcg
    22561 atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt aacaggaaga
    22621 gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc gcctacatga
    22681 actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc attcacgatt
    22741 aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac atagatgtaa
    22801 tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga gcgaacagtt
    22861 tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt ttcatcctcg
    22921 taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact taaatattta
    22981 gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt atacatctca
    23041 gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt catatgttcg
    23101 ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag ataggaatag
    23161 atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc atagatatgc
    23221 atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc ttgagtcaac
    23281 tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc tcgccttatt
    23341 cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg aactccagca
    23401 actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct tgtcttttta
    23461 ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag ccaattgcgg
    23521 aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca actaacgcga
    23581 tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct tcatcagaga
    23641 ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata tgtccatgtg
    23701 ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca aacttctttt
    23761 cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa agcatcaagc
    23821 cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg taaccttgtt
    23881 ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat tgggcaggaa
    23941 tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca tacacgatat
    24001 cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga atcacaatgg
    24061 ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca ttaactttga
    24121 tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct ttttctttta
    24181 aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac tgcatatcaa
    24241 tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg ataagaagca
    24301 aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc ttttgcgaca
    24361 ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat ttctagccat
    24421 tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt attcagtgaa
    24481 gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc gccgacactt
    24541 ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc tctaaaactt
    24601 tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc ccgtttaatc
    24661 gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catccaaata actgatccga
    24721 acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct gtctactagt
    24781 tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt atgcacgccc
    24841 aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa taatgtgccg
    24901 tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat ccctgcttgt
    24961 tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt attatgaacg
    25021 atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa gcgttcgata
    25081 gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt cttggatttc
    25141 ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg cacgctgaag
    25201 tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg aatgctcaat
    25261 cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa ttttcttctc
    25321 accgctgatc agctgtacag gatactcgat caccagatga taagaacggt agccacttgc
    25381 ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc ttttacggat
    25441 gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta acccggcaat
    25501 atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg attttgttac
    25561 aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat gtcgattttg
    25621 ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata cagtttgttc
    25681 atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta cttcattaac
    25741 attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt tatgcattga
    25801 ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga cacaaatgct
    25861 tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca tcgctcacta
    25921 tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata ccgttgatga
    25981 acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc aagctgaata
    26041 tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct ttataacaga
    26101 agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag tttttcaaaa
    26161 actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc ttacaacaaa
    26221 acgagctgaa ttccctattg aagaaggact tttagctatt gatgaaagct ggggagacaa
    26281 tctacacgat tttgaactgg aactagaggt aggggatgca tctgtgggaa aaattgcttt
    26341 tataaatttt ttaaaacgtt tttctttacc ttatcgtcct gctaagaaca agatccaacg
    26401 tatgcttgaa gctaaaaatt agaaaccttt cattttcaca aggcacttga gttcattttt
    26461 ttactttttt ctgttaagtt ataggatgtg aaagaatcta gtcactagtt gtatagaatc
    26521 tattttgggg gagattcata tgatcgaaat ctatttattt gttaatcctt taggggaata
    26581 ctgtttcgaa atggaacaac agttattaca atttatcgaa gaagagtacg ggacaccatc
    26641 aaaagaaaag atgcaatttc gctttcttcc tttggtcaat ttgcagacaa ttggggatgt
    26701 catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa cgtaatcact tattctcaat
    26761 gacttattct gcagcattgg actgcaaagc ggcgcagttt caaggaaaga aaaaaggccg
    26821 ccgttttttg atcaaattgc aagaagcagt cggctgtaat gacataccat attctcaaga
    26881 acttgctgaa tccatatttg aagaaatcgg cggcgatttg aatatgttta aagaagatcg
    26941 tcagtctgac ttcgttaaag aagtattctt ttctgatcaa aaaattgctc gggaaatggg
    27001 catcacaaaa cacccttctg cagtcgttta caattatgcc tgcgaccgtg attttggtgt
    27061 tttagtagaa gtaaaaggta caagtagcct ttggagaatc acagagctgt gtaaaacaac
    27121 agataactca tatcaagttt ttaatggtga acatttacat acaaatgatt ctcagttcca
    27181 ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DACGLH010000030        26212 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.032, whole genome shotgun sequence.
ACCESSION   DACGLH010000030 DACGLH010000000
VERSION     DACGLH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.032"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(167..988)
                     /locus_tag="IXT21_001458"
     CDS             complement(167..988)
                     /locus_tag="IXT21_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ4868866.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1085..1363)
                     /locus_tag="IXT21_001459"
     CDS             complement(1085..1363)
                     /locus_tag="IXT21_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868867.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1631..3640
                     /gene="metG"
                     /locus_tag="IXT21_001460"
     CDS             1631..3640
                     /gene="metG"
                     /locus_tag="IXT21_001460"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ4868868.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(4119..4706)
                     /locus_tag="IXT21_001461"
     CDS             complement(4119..4706)
                     /locus_tag="IXT21_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868869.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            4881..6203
                     /locus_tag="IXT21_001462"
     CDS             4881..6203
                     /locus_tag="IXT21_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4868870.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGIFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            6358..7134
                     /locus_tag="IXT21_001463"
     CDS             6358..7134
                     /locus_tag="IXT21_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ4868871.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            7127..7696
                     /gene="rnmV"
                     /locus_tag="IXT21_001464"
     CDS             7127..7696
                     /gene="rnmV"
                     /locus_tag="IXT21_001464"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ4868872.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            7689..8573
                     /gene="rsmA"
                     /locus_tag="IXT21_001465"
     CDS             7689..8573
                     /gene="rsmA"
                     /locus_tag="IXT21_001465"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ4868873.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            9024..9653
                     /locus_tag="IXT21_001466"
     CDS             9024..9653
                     /locus_tag="IXT21_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868874.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(9777..10886)
                     /gene="ugpC"
                     /locus_tag="IXT21_001467"
     CDS             complement(9777..10886)
                     /gene="ugpC"
                     /locus_tag="IXT21_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ4868875.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            11095..11517
                     /gene="mgsA"
                     /locus_tag="IXT21_001468"
     CDS             11095..11517
                     /gene="mgsA"
                     /locus_tag="IXT21_001468"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ4868876.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            11840..12376
                     /locus_tag="IXT21_001469"
     CDS             11840..12376
                     /locus_tag="IXT21_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ4868877.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(12416..14179)
                     /locus_tag="IXT21_001470"
     CDS             complement(12416..14179)
                     /locus_tag="IXT21_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868878.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(14182..15897)
                     /locus_tag="IXT21_001471"
     CDS             complement(14182..15897)
                     /locus_tag="IXT21_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868879.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(16676..16900)
                     /locus_tag="IXT21_001472"
     CDS             complement(16676..16900)
                     /locus_tag="IXT21_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868880.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            17100..19868
                     /locus_tag="IXT21_001473"
     CDS             17100..19868
                     /locus_tag="IXT21_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4868881.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNWLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            19949..20368
                     /locus_tag="IXT21_001474"
     CDS             19949..20368
                     /locus_tag="IXT21_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ4868882.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            20380..20850
                     /locus_tag="IXT21_001475"
     CDS             20380..20850
                     /locus_tag="IXT21_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4868883.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            20867..21646
                     /locus_tag="IXT21_001476"
     CDS             20867..21646
                     /locus_tag="IXT21_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4868884.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            21633..22454
                     /locus_tag="IXT21_001477"
     CDS             21633..22454
                     /locus_tag="IXT21_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4868885.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            22471..23520
                     /locus_tag="IXT21_001478"
     CDS             22471..23520
                     /locus_tag="IXT21_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4868886.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            23533..24537
                     /locus_tag="IXT21_001479"
     CDS             23533..24537
                     /locus_tag="IXT21_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4868887.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            25170..26189
                     /locus_tag="IXT21_001480"
     CDS             25170..26189
                     /locus_tag="IXT21_001480"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ4868888.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         8517 a         4614 c         5336 g         7745 t
ORIGIN      
        1 aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt ttttattcag
       61 aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa gagttgtttt
      121 tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat tcatggtgcc
      181 ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt tcaccgatct
      241 catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt ttataatcga
      301 tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct ttacctaaaa
      361 cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat gctttcaaga
      421 tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca gtgactaaat
      481 caagcgagat agccacttct ccgctagata aatggatctt gttactatga cgttctagtg
      541 tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct ccaataaagg
      601 ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta ctgaatgtat
      661 gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt gtttctctta
      721 agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat gcccatgaat
      781 ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct tcttctttgt
      841 tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct ggacgttcct
      901 gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa tataattttt
      961 cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta aaagtacttt
     1021 ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta
     1081 tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa agcagataaa
     1141 acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata acgatatata
     1201 cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa tgcccatgga
     1261 ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt gctaatgaaa
     1321 gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt ccagtccctt
     1381 actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa gccgtttttt
     1441 agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc ttttaaagga
     1501 tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa aaaagctaac
     1561 agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc gataaaggag
     1621 gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct attatccaag
     1681 cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa ttgctcgtta
     1741 taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac atggtcaaaa
     1801 aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc
     1861 ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat tcattcgcac
     1921 gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat tagaacaggg
     1981 agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag aattctttac
     2041 agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc
     2101 gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc gcatgagcaa
     2161 atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc agccagaatc
     2221 tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc ttgcagtttc
     2281 acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg tggtgtatgt
     2341 atggatcgat gcattatcca attatattac tgcactagga tatggctccg aagatgaaag
     2401 tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt
     2461 tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt
     2521 atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt
     2581 cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt attatttgct
     2641 tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg tttcccgtgt
     2701 caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg ctatgatcaa
     2761 caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat ttgatagtga
     2821 actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga
     2881 attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca aatacattga
     2941 tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt
     3001 catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc caatcatgac
     3061 agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa tgaatcttga
     3121 aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag gtacaccaat
     3181 cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg
     3241 tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac
     3301 gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt
     3361 catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac tagatgctgg
     3421 agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg atccaagcga
     3481 attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca
     3541 aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac aagtaatcga
     3601 agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag ggggtatgac
     3661 actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt aaattccttc
     3721 ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa taaatcctag
     3781 aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa taggaatcgt
     3841 aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga ttcatctggt
     3901 actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta ttacatctat
     3961 atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt tatgttcaat
     4021 tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc atcggctttg
     4081 acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt atggtaacct
     4141 tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa
     4201 aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt tgtcaacatc
     4261 acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg attatcccaa
     4321 tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata gatcccgact
     4381 aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa
     4441 agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat cccccacaag
     4501 atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag caatattagt
     4561 ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa
     4621 tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata atctgctaat
     4681 ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa ggtaggttta
     4741 gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc actgaaagtg
     4801 gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat accaaatttc
     4861 aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat ctgtttggcg
     4921 cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg
     4981 ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg ggtggtttct
     5041 taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg gaaactggtg
     5101 gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta ttaggatggg
     5161 cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc tttgcgacac
     5221 aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca gtgatcaccg
     5281 ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct gtacaatcag
     5341 tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga ttattgaccc
     5401 caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg
     5461 aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg
     5521 gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca attattcttg
     5581 gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg aaaactttgc
     5641 cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac gtcatttttg
     5701 gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac ggtgcattga
     5761 acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa gacgagctac
     5821 ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat gttcctgcgg
     5881 ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac tttttaacag
     5941 acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt atctttattt
     6001 taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat ccaatcgttc
     6061 cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg acgcaaacga
     6121 aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat tatcaaaaaa
     6181 agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt acgaactagg
     6241 tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat tttgattttt
     6301 gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga gataaacatg
     6361 atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat tccagaaacg
     6421 attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca
     6481 acgattgaaa agtctttgca attaagccat gactatccaa atatatacag tattatcggc
     6541 tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt acaagaacaa
     6601 ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca ttggatggaa
     6661 gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc aaaagaaatg
     6721 aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca aatcctaaaa
     6781 gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga ttatgaatgg
     6841 gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt cactttcaaa
     6901 aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa
     6961 acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga accaggctat
     7021 actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga agaagtggct
     7081 gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg ataagatgaa
     7141 gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc gggaagtagt
     7201 tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc tgatgcaaat
     7261 tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt tttcaggaga
     7321 aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt ttttgccgcg
     7381 cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc
     7441 tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg ctgtttctga
     7501 aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag caaaagaaaa
     7561 acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac aattggaaaa
     7621 acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga aaatagtgga
     7681 ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa gagattttat
     7741 tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact gaaccaaata
     7801 ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg
     7861 gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa gtcgtagcat
     7921 ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc tatgacaatg
     7981 tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt agagaaacat
     8041 ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt acgacaccga
     8101 tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc atgatgcaaa
     8161 aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt tcattatcga
     8221 ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa acagtatttg
     8281 tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat ataccggcag
     8341 tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt cagcaaagaa
     8401 gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag acaaaagcat
     8461 ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg gagactttgt
     8521 cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga
     8581 ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     8641 aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca agcttttgaa
     8701 caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa ggaacaattt
     8761 caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg caagcttttg
     8821 accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc ctatttttgt
     8881 ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg atttagaaaa
     8941 gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat ttatgtgtca
     9001 agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta acgatttctg
     9061 ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc atgattccac
     9121 cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt tttacaccag
     9181 gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca acgccggtta
     9241 tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct tggtacttgc
     9301 aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc
     9361 aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta gtagtcagca
     9421 tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat ttttatacag
     9481 tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat aatattgctt
     9541 attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa
     9601 aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat tagacatgat
     9661 ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga tgagagtttt
     9721 ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg ggcaatttat
     9781 ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct gatgttgaag
     9841 gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt tgagatgaac
     9901 tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc ggaaacgaca
     9961 acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg
    10021 tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc aactaatact
    10081 ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat aacaccattt
    10141 ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc aacgaacata
    10201 ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa tccgtctttc
    10261 atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt aacatagatg
    10321 gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc tacacgtaat
    10381 ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc ccgaacgatt
    10441 gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg ttttcgtttc
    10501 aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt gatgtctgct
    10561 ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt catatgagga
    10621 tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc cacgtcattc
    10681 atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc cgcgatcatt
    10741 cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa ttcacggtct
    10801 ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata ttttttatat
    10861 acgtttttca gagccatttc taccataata cgttccacac tttctttttt agttttatta
    10921 actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga acaagaattt
    10981 tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata agaaataaaa
    11041 actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa
    11101 atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat
    11161 aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca gaggatcacg
    11221 gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg ggatcagcaa
    11281 attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg agacccgctg
    11341 gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga tgtctatgag
    11401 attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact ggaagccggc
    11461 ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc attttgatgg
    11521 ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa aagctaaaca
    11581 aactgataat atgcgatata atacgaacgt attggataat ataaacaata atattagttt
    11641 ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa
    11701 taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg cctcttggac
    11761 gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga tgtagcagct
    11821 cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga ttgctattat
    11881 gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg agaccccatt
    11941 tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt ttggtccttt
    12001 ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct tctcgaaatc
    12061 tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct acggattctt
    12121 tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta gtgtcacgct
    12181 gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg tgccattgtt
    12241 cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac cgattcaatc
    12301 aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa gaagtttagc
    12361 gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct tgtttttatt
    12421 gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga agcagctcat
    12481 catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg tccgcatttt
    12541 tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc attgctaaga
    12601 aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct tcatctaaga
    12661 tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg cgttgtccat
    12721 ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc aaacgttcta
    12781 caaattcata catataagag gcttttaggg catcattgat ttctgcgtca cttgcttccg
    12841 gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca
    12901 ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca tggtcatcga
    12961 ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca agtgtggatt
    13021 ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca gtaaaattga
    13081 aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca aaagagactt
    13141 ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc ttttcttctg
    13201 gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg
    13261 tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc gtaaagctgg
    13321 aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg ccaactaaac
    13381 caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc aaggaagaag
    13441 aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca aattgctgtt
    13501 gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt cgttcggtca
    13561 caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt tgtgaagcac
    13621 gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg accaatgtca
    13681 gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc actccagaaa
    13741 ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca tttgtaaatc
    13801 gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat cgattcagat
    13861 gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga ttgcttaaca
    13921 cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat agaataagta
    13981 aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg atactttgac
    14041 caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt atcacagcta
    14101 gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg
    14161 ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt cctgatattc
    14221 tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac cttgatccat
    14281 cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa taatcactgt
    14341 actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat aatccaacgc
    14401 acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt
    14461 cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt ttgtatctaa
    14521 accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag cttcccaaca
    14581 ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag tcccggtaaa
    14641 tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca gctgccaaga
    14701 acgaacatcc attccgtcca ttataactgt accggaactg acatcataga aacgcggaat
    14761 caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa ggactgtatt
    14821 tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag tataacggaa
    14881 gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag tactgacctc
    14941 agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc ctgaagcttc
    15001 ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa gcatttgatt
    15061 catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat ttactttgat
    15121 cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg tagcaggtga
    15181 taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat ttgtgctttt
    15241 atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac gaacaccgct
    15301 aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt tttggtagag
    15361 aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca ataatgctaa
    15421 gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc cgatacttaa
    15481 aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa gctgattgat
    15541 gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga
    15601 gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc cttgtgaagc
    15661 tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag acatagcgac
    15721 catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc cattatcgat
    15781 caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa
    15841 cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact gaatcatatg
    15901 attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac attgtagctt
    15961 aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt attatgaaga
    16021 agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa aacatgccag
    16081 tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa ttctgcataa
    16141 aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt taatttgttc
    16201 ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa atctaattga
    16261 ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt gggtactatc
    16321 ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta tcaggtttga
    16381 tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt gatctgaaaa
    16441 taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg
    16501 aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag tacctcttta
    16561 ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt tgttatacca
    16621 catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt
    16681 ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt
    16741 cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata gccatataaa
    16801 gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga aaagttttcc
    16861 aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca tagcctcctt
    16921 attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg ttttttagaa
    16981 taaaaattat aagggataag taggctgctg tctataataa gatatatttt tcaattcttt
    17041 attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg gtgaataagt
    17101 tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc gatttagaac
    17161 atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta aaaagaaaca
    17221 cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa atcaattctc
    17281 gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag ttacgcacga
    17341 atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg caaccaaaag
    17401 acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc gaacaaatca
    17461 aaacagctct ttactatcca gatggcggat taccactttt gattacaggc gaaagtggca
    17521 caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat gatctgctag
    17581 aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat cctgaacttt
    17641 tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa gaaaataaaa
    17701 aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta catcgcttaa
    17761 caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt tatcgtatgg
    17821 gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg actgaagaga
    17881 ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc gaactacctg
    17941 cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt tttctcgaag
    18001 aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca cttttaacaa
    18061 gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa
    18121 aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat catttgcaaa
    18181 aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc tatattacgc
    18241 aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag cgaatcgtta
    18301 aaagttttga gcaaatccta attgatttca agaaaaataa ctggctgcta tattcttgcg
    18361 aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt gagaccagta
    18421 gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag acctttcgac
    18481 aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc ttgagttact
    18541 atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt atccaactaa
    18601 tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac tatgtccaac
    18661 gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg gatcggattt
    18721 tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga
    18781 tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta aatcgtttat
    18841 tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca cagcagattg
    18901 cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg atcatattag
    18961 tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta tccgtaccgc
    19021 ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa tgcctgcaaa
    19081 aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag ccagaatggc
    19141 gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc ttcactggaa
    19201 tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg gcttgtcagt
    19261 tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt gacccattat
    19321 tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc accaatatcg
    19381 actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta gctgaatggt
    19441 tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc attcgtaatt
    19501 tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag gtgatgggag
    19561 aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc agcaatccca
    19621 aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc cgacaaatgc
    19681 caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg
    19741 ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc cccaattctg
    19801 agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg atagaagaag
    19861 aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc ttttttagta
    19921 tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag cttcccatgg
    19981 aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa atcattctat
    20041 tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag taaggcagct
    20101 ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg gcggaagtgt
    20161 caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg ccggattgaa
    20221 tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga caactgtttt
    20281 gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg agcggctcaa
    20341 tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt gacaagagtc
    20401 gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat aggcgcagac
    20461 tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac aacgatcaaa
    20521 ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc gattgccgcg
    20581 ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc gatcgaggat
    20641 gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg agggatcaag
    20701 gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga agaggaggaa
    20761 cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt accagcagat
    20821 aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga ttgtacaagc
    20881 agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag ggacatcgtt
    20941 gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac
    21001 tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct cagtaggtgc
    21061 ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg ctattacagc
    21121 tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt tgacattgat
    21181 attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag cagacaatta
    21241 tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg gtatcggtct
    21301 ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca atacgatcgg
    21361 aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt
    21421 gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc
    21481 atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga ccggagtagc
    21541 tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc gtcaactacc
    21601 acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc aaaaacagca
    21661 aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg gagttttctt
    21721 ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg attttgaaaa
    21781 agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta gagtttttta
    21841 acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg gaggaagaaa
    21901 ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca tctttgatgg
    21961 gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg attgccacag
    22021 gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg ttcattttga
    22081 ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga tatcgcttag
    22141 gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg acattaggcg
    22201 cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc accatcaata
    22261 ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata
    22321 tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta ttgaagaaag
    22381 aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt ggttcgtgga
    22441 tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta tatctatgaa
    22501 gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag agaacatatg
    22561 aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc ttgtttagca
    22621 gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga acctttttat
    22681 tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt gtcacaaagc
    22741 gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg gttacctgtt
    22801 gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca aattattgat
    22861 ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc gactgtttta
    22921 cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag caaaaaaata
    22981 gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc gattattcaa
    23041 aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca gcgtttcgtc
    23101 accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa attgactgaa
    23161 acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg cccatacctg
    23221 gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa taaggaacgt
    23281 tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac cattaccaca
    23341 aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga atatctgtct
    23401 gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt agctaaaggt
    23461 attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag taaattataa
    23521 gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca
    23581 ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga aaaatttgac
    23641 gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc tgtcacttat
    23701 atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata ttacactact
    23761 ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac cggtacaact
    23821 caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact gattggattt
    23881 atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac ttatccagct
    23941 cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta tttgaatgga
    24001 gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc aacagggtta
    24061 gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa acatcgacgt
    24121 gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta ttcatatgcc
    24181 atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc tgccgagttt
    24241 cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt tttaggcgaa
    24301 gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat
    24361 tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa atatcgcgga
    24421 cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc tcatgtggag
    24481 cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga ttattaggat
    24541 agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc cctttatttt
    24601 tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc tacagttcaa
    24661 cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg caatagtact
    24721 atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc gccagtttac
    24781 atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa ccgtcaacag
    24841 agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg tctcttatgt
    24901 attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa tgctggtagt
    24961 aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg taaaaataaa
    25021 atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa agaagtaaac
    25081 aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact ataatggaaa
    25141 ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt cttggtcctg
    25201 gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat gtccgtattt
    25261 ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat cagcattttt
    25321 tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca gaagcagtgg
    25381 ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca gtcgcacagg
    25441 aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa ggactagaac
    25501 aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa gaaaaaagac
    25561 aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta catgatatca
    25621 caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa aaactgttca
    25681 tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa actggtgcag
    25741 cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga ttcggagatg
    25801 atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt ggtgtcgcga
    25861 tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg atcgttactt
    25921 gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag ggacataagc
    25981 tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg acaaaagcag
    26041 cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca atctataatg
    26101 ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta cgtgacggaa
    26161 aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc gt
//
LOCUS       DACGLH010000031        25615 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.033, whole genome shotgun sequence.
ACCESSION   DACGLH010000031 DACGLH010000000
VERSION     DACGLH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.033"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            110..973
                     /locus_tag="IXT21_001481"
     CDS             110..973
                     /locus_tag="IXT21_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4868889.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1102..2724)
                     /locus_tag="IXT21_001482"
     CDS             complement(1102..2724)
                     /locus_tag="IXT21_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4868890.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2973..3494
                     /locus_tag="IXT21_001483"
     CDS             2973..3494
                     /locus_tag="IXT21_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868891.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3680..5410)
                     /gene="spxB"
                     /locus_tag="IXT21_001484"
     CDS             complement(3680..5410)
                     /gene="spxB"
                     /locus_tag="IXT21_001484"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ4868892.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5575..6393
                     /locus_tag="IXT21_001485"
     CDS             5575..6393
                     /locus_tag="IXT21_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ4868893.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6496..6693)
                     /locus_tag="IXT21_001486"
     CDS             complement(6496..6693)
                     /locus_tag="IXT21_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868894.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6862..7440
                     /locus_tag="IXT21_001487"
     CDS             6862..7440
                     /locus_tag="IXT21_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ4868895.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8485..8988)
                     /locus_tag="IXT21_001488"
     CDS             complement(8485..8988)
                     /locus_tag="IXT21_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ4868896.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9347..10372
                     /locus_tag="IXT21_001489"
     CDS             9347..10372
                     /locus_tag="IXT21_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4868897.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10719..11597
                     /locus_tag="IXT21_001490"
     CDS             10719..11597
                     /locus_tag="IXT21_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868898.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12073..12189
                     /locus_tag="IXT21_001491"
     CDS             12073..12189
                     /locus_tag="IXT21_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ4868899.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13117..13455
                     /locus_tag="IXT21_001492"
     CDS             13117..13455
                     /locus_tag="IXT21_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868900.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13549..13779
                     /locus_tag="IXT21_001493"
     CDS             13549..13779
                     /locus_tag="IXT21_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868901.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14179..14550
                     /locus_tag="IXT21_001494"
     CDS             14179..14550
                     /locus_tag="IXT21_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ4868902.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14824..15516)
                     /locus_tag="IXT21_001495"
     CDS             complement(14824..15516)
                     /locus_tag="IXT21_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4868903.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15519..16304)
                     /locus_tag="IXT21_001496"
     CDS             complement(15519..16304)
                     /locus_tag="IXT21_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4868904.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16541..17941
                     /gene="lacG"
                     /locus_tag="IXT21_001497"
     CDS             16541..17941
                     /gene="lacG"
                     /locus_tag="IXT21_001497"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ4868905.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17966..18283
                     /locus_tag="IXT21_001498"
     CDS             17966..18283
                     /locus_tag="IXT21_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4868906.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18296..19969
                     /locus_tag="IXT21_001499"
     CDS             18296..19969
                     /locus_tag="IXT21_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4868907.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19983..21614
                     /locus_tag="IXT21_001500"
     CDS             19983..21614
                     /locus_tag="IXT21_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ4868908.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22486..23676
                     /locus_tag="IXT21_001501"
     CDS             22486..23676
                     /locus_tag="IXT21_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ4868909.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23810..>25615
                     /locus_tag="IXT21_001502"
     CDS             23810..>25615
                     /locus_tag="IXT21_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4868910.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQ"
BASE COUNT         8130 a         4270 c         5107 g         8108 t
ORIGIN      
        1 ttattttgat tagaacttgc gaaaataaac cggttaaagt gtgtctagcg caagagattt
       61 gctataatag acgataaggg taaaatgtaa cgagaagggg ttgaaaaatg tgtatcaaat
      121 tgtaacagat tcttgttgtg atctgccata ccatgtatta gcaaaagaaa aagttgattt
      181 tttatcaatg aatctccaat tgaatgatca agagttagta gatgacctcg gaaagacatt
      241 caattatgac cgctacatga atcagctgaa aaatggtgcg atgcctacga catcacaaat
      301 caatgttggc cgatatttag aatttttccg ttcttatgcg gaaaaacaga ttcctgtttt
      361 atatgtggct ttttccagtg gattaagcgg ctcttacagt agtgcgcttc aagcggttga
      421 attgctgaag gaagaatatg aagaccctga gatctatgtt tttgatacaa aagctgcaag
      481 tattggggaa gggcttttag tattggaagc tgtaagattg aaaaaagaag gtaaaagcat
      541 cgaagaaacc ctagagtggc tggaaagtaa ttatttgaaa gtccattcat gggtaacagt
      601 agacgatctg aaatatctag aacgaggcgg taggatttcc aaagcaagtg ctactcttgg
      661 cagtctattg agtgtaaagc ctattattac ggtggataaa gaaggaaggc tgacgaatgt
      721 tgacaaagtt cgaggaagaa acaagtcgat ccagacaatc gtcaatgaga cggtaaaaac
      781 aatcgttgca ccacttgatc aaatcgttta tgtggcttac gctggggatc tagagagcgc
      841 ggagaaggca aaacggcttt tggaagagca gataaagatg aaagatgtga agctttatcc
      901 cttaggacca acgatcgcca gtcacacagg ctatggctgt atcgcgattt tttcgatggg
      961 aatcagtcga taaaagcagt aactcattct gtaaaaaagc ccagaaattg attgtaacaa
     1021 aaacagcgac tcttaaaaga tagatgagca tcctatctct tagaggtcgc tgttttttta
     1081 ttttttaagc taaataggcc attatgccca taactcgttc aattgttttt gaatatctgc
     1141 attttccaaa aattcatcat atgtcgtatc agctcgatcg atcacacctt tatccgaaac
     1201 ggcaatgatg cggtttgcca atgtttggat aaattgatgg tcatgtgacg caaataagat
     1261 tgagccagta aatgccatca agccgtcatt caatgctgtg attgattcta agtctaaatg
     1321 gttcgttgga tcatctaata caagtacgtt agctttcgaa agcatcattt ttgaaagcat
     1381 gcaacggact ttctctccac cagataaaac attcactggt ttcaatactt cttcaccaga
     1441 aaacaacatt cttcctaaga agcttcttaa gaaggtgtta tcatcttctt ctttacttgc
     1501 atattgacgc aaccagtcta aaatcgttag atcagtatcg aattctttcg ttgtatcctt
     1561 tggtaaataa gaattacttg ttgtcacacc ccaacgtact gttccagtat ctggcgtaat
     1621 ttcacccatg ataactttga atagcgttgt tgttgttata tcattttctg cgacgaaagc
     1681 gaccttgtct tctttgctca aattaaaaga tatatcatct aaaattttct ttccatcaat
     1741 cgttacactt acattttcta cctgcaataa gtcgtttcca atctcacgtt caggtttgaa
     1801 tccaacgaat ggataacgtc tagaagaagg ttggatatcg tctaaagtaa ttttttcaag
     1861 cattttttta cgtgaagttg cttgttttga ttttgatgca ttcgcactaa agcgggcaat
     1921 aaattcttgg agttctttga tttgttcttc ttttttggca tttgcgtttg cttgaagttt
     1981 agccgctagt tgactagatt ccaaccaaaa atcataattt cctacataaa gtttgatttt
     2041 accaaaatct agatccgcca tatgtgtaca tactttgttc aaaaagtgac ggtcatggga
     2101 tacaacgatc acagtatttt caaaattgat caaaaattct tccagccaat tgatcgattg
     2161 gatatccaga ccgtttgttg gttcgtctag caaaagaaca tcaggtttac caaatagtgt
     2221 ttgtgctaat aacactttta ccttttgtcc accagttaat tcactcattt gctgatggtg
     2281 caattcttca gggatgttca gaccttgcag taaaacagct gcttctggtt cagcttccca
     2341 tccattaagc tcagcaaatt ctccttctaa ttcagctgct cgaataccat cttcgtcagt
     2401 gaaatcctct ttcatgtaaa tcgcatcttt ttctttcagc acttcataca gacgcttgtg
     2461 tcccatgatg actgtttcaa gtacagtata gtcttcatag tcgtaatggt tctgtttcaa
     2521 tacggccatc cgttcatctg gtcccatagt aacggaacct gtggaaggct gcagatcccc
     2581 tgataacact ttaaggaacg ttgatttacc tgcaccattt gcaccaatca agccatagca
     2641 gtttccgggg gtaaatttaa tattcacatc atcaaatagt ttgcggtctg aaaagtgcaa
     2701 actcacatca tttacagtaa tcaatttttt tcctcacttt tatcattatt agttttgaca
     2761 agtcttatag aattatatag gggaaaggta aaagattcaa ggcaatcgaa cattctttgt
     2821 gtttcacgtg aaacagaatg aaaaaaataa aaataaaagc ggagcgaaaa tagctactat
     2881 aggatttatt agtttgcaac agcataattt ttgacgttat ttataagagg gagtactata
     2941 aaactgtaaa agaaatgaaa gaaggattga aaatggatga aaaaagaacc atcgactggg
     3001 gctcattgat tttgggaatt ttatttgtac ttgtctcatt gttatcattt caagaccctg
     3061 tcggaaattt agttgcaatt gtagtagtct ttgcgatttt tgcctttatt aaagggatat
     3121 ttgaactgtt tgtacggaac agattaaaag agctaacagg atataaagga aagatgccct
     3181 taataatcgg gatcatcgat attctagttg gcgtcttttt cttattcaat attggtgcag
     3241 gagtcgcagc attgcctttt gtttttgcag tatggtttat tgctgattca gttttagcgt
     3301 tatttactgc tgacttggca agaggagtaa gtgaaggata ttattggttt acgatcatcg
     3361 tcaatatttt aggaatttta ttaggaattt tcttattgtt taatccaatt tcttctgcgt
     3421 tgacattaag ctttttagtt ggattctatt ttatgttatt tggtatcaca catatcgtct
     3481 atgcatttag ataagtaaaa tcaaacaaat aaaaaagccg gcttccgatt acagttttac
     3541 tgttgtaatt ggaagccggc ttttttatca tggacaaata tgcacgaggt gatttttagg
     3601 tgtcgagaca aatttatgca aaaatgtgag aagctatata cacaaatttt atcttaatac
     3661 tcgaagcata gcgcagttgt cacaacctct tttcatataa ttcattcaat actggcaaat
     3721 catggacttc atatttttct ttgaatgctt ggatttcttc ttcagaatat tttcttggat
     3781 ctaaatgcag atcttctact ggcaatgggc gcttgttatt gatcttcaca tcgattacta
     3841 caggacgatc tgattttgca gctgcatcaa aggcaaatcg aagttgcgag tattctgtga
     3901 ctgtaaagct atttgccccg agcgcttctc caactttacc gaaatctgca tcttctaaaa
     3961 atacaccaaa tttcttttgt tccgtatctt cttgttctgc ttcgatgaaa ccaaaagaat
     4021 cattcgaaaa gacaacatta ataattggaa gctgatattt gacttgtgta atgatatctt
     4081 gcatgaccat agcaaacccg ccatcaccac ttaaggtaaa tacttgtttt tctgggtagc
     4141 ttagttgtgc ggcaattcct cctggcacac cattacccat agttgcgaac catccagatg
     4201 tcgtatgttt ttgtttaccg ttcatattca ataaacgaat agaatggata gtcgtgttac
     4261 cgacatctgt gacgaaaatc gcatcgtctt cagcaatacg attgatttct ttatagacag
     4321 cctctggacg aatcggttct tcctctcgat cttcaaaaga tttcaaccaa tttacccatt
     4381 tttctttatt ttcttgattt gctaggtacc agctatcagc agggcgagca tcccctaact
     4441 cgacaagtct ttgcaaagct gtgtttgcat caccgaggat cgacaaagat gtttcatggc
     4501 gtcgtccaaa ttttgacgca tcgatatcga tttggataaa ttcagcagtt ggattaaaaa
     4561 atgcacgtcc aaatgggaag tcgctaccga caaataagat cagatcggct tctgccaatg
     4621 cttcatttcc gggtttagta gcgacacgtc cagcaaaacc aagaaagttt tcataatcat
     4681 caggaataat tccttttgct agaacggaag cgacaacagg aactgaaaaa tgttctgaga
     4741 attctttgat ttgcggaaat ccgtttcgcg ttccttgacc aacataaaga actggttttt
     4801 ttgctttttc aagataaggc aaagcagcta gcaaatcttt ttcttctggt aatatcacac
     4861 ttgttttgtg agtgtgagcg gtggagaaag gttgttcgtc aatttcttca aaaccaagat
     4921 caacagggat agtcacgata gccacgcctt tatgctcata ggcggcttta atggcttcat
     4981 ctactacatg cggaagactt tgtggtgtca tgactgtacg attgtatacg ctaacatctg
     5041 cgaaaatagg attttcattt agttcctgga aagagttata gttcatggat gtagaagcaa
     5101 cttgtcctaa gaacgctact agaggcacat ggtccatctg tgcatcatac aaaccattga
     5161 tcaaatgtgt cgcaccaggt cctgcagagc cgaagacagc gccgactttc cccgttaatt
     5221 ttgcatctgc agcggctgcg agtgcgccga cttcttcatg acgaacttga atgtagttga
     5281 tttcggtttt ttcttgatat agcgcatcca taattgaatt gaaagatcct ccgggaatgc
     5341 cgtaaatatg gtcgataccc cagctttcaa gaacttttac cattgcgatt cctgcattga
     5401 tttttgacat ggtgtttcac tcctttagca atgatgatac ataaattttt tgtatctaga
     5461 gtatagaact ttttatcgaa aatcccaact aattcgtctt ttcacaaaaa tgtagtcatg
     5521 tttttgggaa tatgctagag tagtacatag atagattcaa gagaggggaa tggcatggtt
     5581 gaatggctat tgagacttga accttggcag caagcacttg ctgggacagc ttttacttat
     5641 tttatgacag ctcttggtgc cgggttggtt tttttcttta aagaaatcaa aaaagaagtg
     5701 ctaaacttga tgttaggatt tgcttctggt gtcatgattg ctgcaagttt ttggtctttg
     5761 ttagatccgg ctattacaaa agcagaagaa aatggagata ttgcttggct agttgtaagt
     5821 attgggtttg gtttaggcgg tgtgttttta tatatggcag ataaaacact gccccatatg
     5881 cattttggac cacaacatga agcagaagga ttgcctaccc atttaaaacg gacgatcttg
     5941 cttgtttttt ctattacatt gcataacatt cctgaaggtt tagccgtggg ggtagcattc
     6001 ggtgcagcag caactgcgga taatccgaca gccgctatat tagccgctgt ttcggtggcg
     6061 ctaggtattg gtatccaaaa cttcccagaa ggagcagctg tatcaatccc gttgcgacaa
     6121 gaaggtctga gtcgtaaaaa ggcctttgtt tacggacaag cttctggaat cgttgaacca
     6181 attgctggag tgattggagc gctactagta acgaaagtgg agctgctgct tccttatgca
     6241 cttgcttttg ccgcaggagc aatgatttat gtcgtggtgg aagaattgat ccctgaagca
     6301 cagcagacat tgtccagcaa acgtcatttt gcagtttttg gcgtcatgag tggatttatc
     6361 attatgatga ttttggatgt ggcattagga taaataaaaa aaatatatat gatatatcaa
     6421 atagggccgc gaacttatat cgcgacccta tttttttagt aagttatatc ataatgaagt
     6481 gaatggtatc atacattaat aatagttttt tctatgtgta tttcctttga tccagcgaac
     6541 aagccagatg ataagtgcga ttgggattag aactttgacc gcaaaccata aaagcccgct
     6601 taggacactg acaaaaagac tgatgacgaa gctagctgca attagcacta aaagaaccat
     6661 gaagatattt aagaacatat ttttttgacg catacaaaac cctcctcttg ttcattcttt
     6721 actatagaaa aagtataaca gggattgttt aaaaacacct ccaactgaag actgattttt
     6781 acttcatcta cttaaatgat caaacaattg ctttttctct ggtttatcat ctaaaataga
     6841 gcggaaaagg aggagaatat catgcctaaa aatcgtcttg aagcatttac agatgctgta
     6901 atcgcgatta tcatgactat tttagtttta gaacttcatg aacctaacgg agacacgtgg
     6961 gaggcgctcg ctgcattagg ttaccgattt tttatctata ttgtcagttt tatcatttta
     7021 gcgatttatt ggaacaatca tcatcattta tttcaagttg tacataagat aaatggacgg
     7081 gtcttatggg cgaacagttt ctttgttttt gcgctttcgc tttttccgtt tgcaacttca
     7141 tggatcagtg agtacatcaa tagcctggct ccagaaatga cttatggttt tgttaccttg
     7201 accgctaata ttgcgtatta tatcttggca aaagaacttg tcaaagcaaa taaaggccac
     7261 gagaaagcat tgaaggcgat gttgaataat tacaagaaat cgtatctatc gattggtttg
     7321 aatgtgttag gaattgtctt agggtattta gtagcgccag tagccgtgct gatcgcaaac
     7381 atcgtgattc tagctgcttg gatcatccca agtcgtacca tcgaatcaca atatcggtaa
     7441 aatgatccgt gtttaaaatt taagagaaaa atatagaaaa aataaataaa cgatcaaata
     7501 tccgagtgat actagtattc aaagagggat gcagatggat caaaaaagaa ttcatgtagg
     7561 actgattact agttttgtca cgatgcttat ttatttggat caaggtcatt tgacttggac
     7621 agtagttggt gtcgtaggat ttttgacatt attcacagca ataaaaaaat aaacatcttt
     7681 atgtaatctg atgttctatc tatttaagaa aagcatgttt acagcagtat gattctgttg
     7741 taaacgtgtt tttatttgtg gaaaatttaa tttttagaaa agtgtatcga aaaatagata
     7801 gttagttttt ccacagtgga taacttcgta agagataaaa ataaaaaatt agtcaaagaa
     7861 gggcgtatag atttcttttg ataggagata tacaacagaa atgaataaat attaacgtgt
     7921 tttcaaacag tataaaacgg tgtggttttt ggaataaaaa aataacatat ctcattttac
     7981 gcttttgtta ttttatactt aaaaaataac gacacagaaa ataccgtgtc tcagatttct
     8041 ctactacttc taaaaactac tgtttttttc ctttgaaatg ccttcaaaaa gtaggatcag
     8101 gcagaatata ctggcagaat caaggagaag ggagcctaaa taaggagaaa aaccatagaa
     8161 aatataaagt aattacttat aaaacaagct ttttatagaa aaataatata cctgagtagg
     8221 gtataaatta agacggaatt cctttctaga ttgagatatt gactaggaag aatgttaaga
     8281 ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa taaggggaat atagcctgtc
     8341 agtttcaaac tgcaagtcgt cctgctgcaa taatatagta gattagagat gaaaatagat
     8401 ttttttaaac caatgactga ataaaaagac cgtcatagat acacattgga atgtgtctat
     8461 tacggtcttt ttttacgaac gattttattg aagacggaca gttcgggtca aaaataaaaa
     8521 agctacgatc agaaataaga ataaaatgta tcccaaaata atggctagac ggtctaactt
     8581 atttgtcgat agatgtttgt ttagaagttg ataggcccat aaacctatca gtcctcctgt
     8641 tgtattagtt atcaaatcag tgatgtccgc tacgccaata ccgaatagga attgaatcaa
     8701 ttctgccaag aagcttacaa ggaaaataat ccctaatttt ttagaaaaag aaagtctctt
     8761 gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca atcaagttat aagtggtctc
     8821 acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa gggacgagat tgacattgct
     8881 gtaagaataa gtgagaaatt gtggaagatt aaatgatagc ttgaataaaa tcaaccataa
     8941 tagaaagaat aaataaatcc ctaataaaaa ttttgataca taccgcatag acatctcctc
     9001 catttaaaaa gtataccatc gaaaaccgct gaaaaatcat aaaaaagtat gaagtttaag
     9061 gaccaacgta gttatcgtac gaaaattaga agcttttttt tgttgggaat ccggtttctt
     9121 taacaaagct aagatagcga gttgcttgga aaagttgcta tcttttttct gtcaaaacga
     9181 ctcggaatgt tcgttttttg ttctaatttc tccaagttgt ttactttttg atactaagtt
     9241 tatgaaaaat caagaaatag ttgttatact aggtaatgct gacaaacggg attggcattt
     9301 catcttggtt cagctttttt tccaattact aggaaggagg gatagattgt tcaagactta
     9361 ttttcgtttc aataaaatac aagataatcc ttttcgtatc atgaatctga tgctacttat
     9421 ctttattgta ttatggttag gttacataaa tcatgatttt ttgatttcaa gtttttcatc
     9481 attagggatc tttacttttt tctattatca aaacataccg atgaaatatt tgctgaaacg
     9541 tatgtttctg atcggttgcg gactgttggt tgctttcgtt ttggggatct tatcgactta
     9601 tgttttatgg cttgaaccat ttgccgtagc tttggttgct ttttccagca gattcgtgtt
     9661 gcgattattc catatttcta aaccaggtgg cttgtttttt gcgatgcttt ctgccatggg
     9721 aacgagcatg cagctgccaa tcgctcaatt accgatcgtc tcactttatt tttttatggg
     9781 tgtcgttttt gccctgattg cggcagtgat tacaaaatta ctggattcac ggcctgagca
     9841 gacaattgaa aaagcaactt taaaagaaag atttcacgaa gagcctttag tgatcattga
     9901 ttctgttttc tacagcgctg ccttatttct ttccgtttat gtcagtcacg gcttgaatct
     9961 gcataatcct tattggttga ccttgtcatg tgcatcgatt ttgctagcag aaaacttgga
    10021 tgcgatgaag catcgacaag tccaatatct gattggatcg atgggtgggt tatgtgtgtc
    10081 ggcattcttg tcatttgtgc cgtttacgca gttgcagacc atttttctga tcacttttct
    10141 atatggaatc gcacaatttc tagttgctag aaattatgca gttgctaata tttttcttaa
    10201 tccgatggcg ttgatgctat ctacacttat tcgtggagca tacttgattt ctttgatcga
    10261 atatcgtttt ttagggatcg tcataggaag tttcatcggt ttaggtgttg cgtgggtaat
    10321 gacggttggt ttgcaacatt atttaacggt tgttcgtcaa aaattagaat agaaggacaa
    10381 atatatgtgc caaaagaggt tatgacaaga tttttgtcac aacctctaat tcaaggtgtt
    10441 caaaagccag attctcagca ttaagtcaaa ttcacacaaa aatgtggaaa ggaactttca
    10501 aaaaaatttt atatttattc tatcaaaaaa ttttatattt attctatcaa aaaattttgt
    10561 ttatgatggt aactgaataa actttcctga gatggtcaat gctttccttg aaagtcttac
    10621 agatattctt tcttcataga aagtttaagt tttatagtta gtgtacaata aaagtatgtt
    10681 tataccatat ggtgtccgga taatcagtca tttaaacaat gaggttcaaa gatatgaaaa
    10741 ggggaaattt gatgaagaaa ataagcaatg aaatccaaat tctggcagaa actttaactc
    10801 ttcccaaagt cgttatagag caaatgattg tacaagcaaa aaacatcctt gatgaggtgt
    10861 ttgagcaata tagtcatgaa ctaatggatc ctaataaagc aaaggcgact gcaaagaaaa
    10921 tagacaaagt ctatgaagag gaaccttttg cagcattagc tatttatcta tatgcagctt
    10981 ctcaaagttg gaaaaaaatt tatcaaccat tgaagattcc aagaactatc tatttggcaa
    11041 caatgaatgc ttttactaga tttattcatg aacatttcca agtaacagga agctatcgct
    11101 ttgaccgtgg tttttggatt tggcattaca ctagtggctt gatctttcgg attggtgaat
    11161 tggagtacga aatgacatat tttccgaaag ggtacacgga tctaagatta gaaggaaagc
    11221 agtgcctttc tcttcatatt ccttccgatg cagatttatc tcagaataag ataagagata
    11281 gttacgaaag tgccattcgt ttttttgaac tttattttcc tgattaccat taccaagtga
    11341 tgtacacaga tacttggtta ctatcaccca atcttactaa atggttgaaa aaagagtcaa
    11401 aaatctgtct ttttgcagcc gattatcgat tattatcagt tgatgaacag gacgatagtg
    11461 gagtcccttg gatctttgga cgagtagatg cccaaataca tgattatcca gaaagtacga
    11521 gtttacagcg acaagcaaaa gagcaattgc ttgcaggcga gcacattggc tctggcttag
    11581 gtattcttac ttcataagtt tagtacttcg aaatagttta aaagagcgtg agacaaaagt
    11641 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc
    11701 ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta
    11761 tttcttggag ttaaacgctt ctgtcccagc ctctttttta gtgttctatc gaacacacag
    11821 tacgttcaat aggtaaacta gctataacaa tagatattaa agttaacgga caagagggcc
    11881 ttttttaggt agctaaaacg taataatgaa aatttatttc cagagatagg agtcaatcag
    11941 ctagtctgat cgactgtttt aacaagttga ctttcatttt aacttttgat accatttaaa
    12001 acacaaatcg taattgtttc gatttgtgtt ttttagcatg actacaatca aagaagaaag
    12061 tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat accgccgctt gaaaagccca
    12121 aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg gaatagtggg
    12181 aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt ttcggctatt
    12241 ttcaataatc ggtttttgtg taaggtttcc gaaagaactg ctgttgattt tccattttta
    12301 acatatgata aattggtaga atctttcttt tcaaattgat accattccct aattccagtt
    12361 atatagttaa ctacagaacc acaaagcacg tacgtttata aaagtattat ctgtagttat
    12421 ttttcaattc cctttcaacc tcttattttg accagtagta ttgatcactt taccttttaa
    12481 cacctttgtt cgtttttgaa acatttttag tcaagaatgg aaacgtaact acataatcaa
    12541 aggttttgcg aaatatttca tgaaatattt catgtagaag cgagcaatac ggttgcaaat
    12601 aaccattatc gtttaataag cagttacgaa agctcccaat atctctaacg ttcagctcac
    12661 tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg tcgaaaaagt
    12721 taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag ttactattgc
    12781 tagaataaca tcaaggaata ataagttttg gttttcagat aatagtcgat aagttgtgta
    12841 ttggaatttt agatacagta ataaagtatt tttcatgaaa ttttgtgaga atcactatta
    12901 caagtatctt taataaaatt aatggttaag gaggaactta aagtagaatg taaatgaata
    12961 gttgtagtca gtcgattgtg atactagact caaagttgca ggaataagtt tttcaatttg
    13021 tgattggaag ataaaaaatg aaaaagaagg gctttttttt atactttgtt gattgtagtg
    13081 tttattttcc ctctgtttag ccaagtaaca ttttctatga ctcaaccagt ttcaagtgag
    13141 gaacatgata acctttttcg tgctacagaa gagtttaaga aatatcagga aaatattgtt
    13201 ccggagactc cattagctag tgagccaatt actgatattg atggaaacca aataggatgg
    13261 tatatacagt acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa tacagtaggc
    13321 ttagagctaa caccgattga aaaggtgagt tcattgttaa cgtatattta tactgatgaa
    13381 agtgacgaga ctgagatcat tttaacggat tactcaaaaa ttgctactga taaaaagtat
    13441 atattgtgga tgtagacact agtgaaatac tttatgatat agaaattgat gaaaatttac
    13501 tagatgtagc acaagaaatc gatcatcaaa agcaggaagc tattcgatat gctggtagaa
    13561 aagggaaaag aaaatattta ttacgtgaat gtagcaaaag ttagagaaga tgaaaatgag
    13621 tggaaagaat tcaagagtcg ctactcaatt aactcgactc cgacatttac agtttacaga
    13681 gaaggatcga tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc tttagctgaa
    13741 gtagaagagt ttttggatta tgttagtatg caacagtaag ggagttctta ttatgaaaaa
    13801 ttttgacaca tattatgtta ttgttttttc gatatctttg ctgttaatat tactaacttt
    13861 aatgcagtat aaagagtatc cagagtattt tattttaact tttgtatatt tagtaagtag
    13921 ctacacactt ctatataaat tcagaagttc ttctagagtt tctaaagttt gtttcttaat
    13981 tagcttattg attctataat atttttgatt tatataggaa attaaaaata ttatagaatc
    14041 aacactcaga gaacgggttg gtttttaata gatttaataa gtactgataa tgaaatttct
    14101 cctggttggg aaaacgaagt tggttaccct gtagaagaat tttttcaaga ttactttgcg
    14161 gagtatgccg ataggataat gagagattac gtaacagata aaactcaaga agtcatcaat
    14221 caatcccagt cattaaatga attatctgat aaggcgaaag aagaagcagt tgccattatt
    14281 tcttcaggga ttagtgacta tattgatatg agtggtgaaa tcaattatgg cgataaagac
    14341 aaaattgtac gcatgataaa agcttttatc aaaacttttg atgtgaaggc agatcttaag
    14401 tttaatgata cttacctagt aggaaaatta attaatatcc ttgggacaga gaaagatacg
    14461 gaaacgttta tttcctcttt gtctatgttg tttacagata agcatttttc aactcagctt
    14521 ggtggagagg aactagtgga aatcttttaa ccattacgcc cagcattaat aaaagtatat
    14581 caaaaaagta ctcctgatga tttttaagat tggtttgaga aataatgtga aaacactatt
    14641 tttaatgtta attatagata gagatgaacg cgtcatatgg ttaaaagaat gaaaaagtca
    14701 aagatacact gctatatttc actaaggata tagcagtgta tctttcatgt cgtttcactg
    14761 gcagtttttt ataaaaggag gtgtgcattt cagttgcttt tctttggtag ttagttgaat
    14821 cgtttaagaa ttaacggatg tggcagacag gtgctctgtc caaaaaccag taaaatcttt
    14881 gatggttcca tctttattta gctgtgcctg ttcaaatact gttttgtttt taccgaatgg
    14941 atcaattgca ataattgttc cgatccccgc acgtttggca gcaagtaatc ctgagtaagc
    15001 atcttcgata acaagacaat cagccggagc taagctcagt ttgtctgccg ctttaagaaa
    15061 aatatccggt tgcggtttcc cagggaaaga gccgtcgtca tagacaatct tgtcccagtc
    15121 aaaccagcgg gctaaatcga aaatatcaaa ataaaaagca acattctctt tgacagtagc
    15181 cgtggcaatc gttaatggaa tctgttggtc agtcagctgg tcaagagtcg cttcaagccc
    15241 ttttgtcaac tgaagctcgt tttcattttg caagcaaagc atacgataat agctttcttt
    15301 ttcttctgac aatacggcga tctcagaggc ggtcaaatgc tctgaaatga aatgtttcaa
    15361 aatctccgta ttcgtccgac catgaatatt ttttaaaata tcctcgtctg ataaaccatc
    15421 ttttgtatga tcatgtatca tatgcagcca agcagcttca tgcagatgag agtccaaaaa
    15481 cattgttccg ttgaaatcaa atataatccc tttcatagtt acgctcctat agtggtattt
    15541 tcggttgaat cagcaatttg cagatcgaca tattgtgcat agtgctcttg aacctcccgc
    15601 gagatctggc gatccgtaat gagcagatcg aaatcatgca aattataaaa cacataaaaa
    15661 tcatctcgat tgaatttttt attatcaacg agtagatatt tcttacggga attatttaga
    15721 gcgatctgct gtgcttctcc ttcttcggta ctgtaagtag agatcgcttc gttgtgaacg
    15781 ccatttgaac tgataaatgc ttttgtaaac tttagtcttc ttaaattgtc gttggtgatc
    15841 ggtccgacaa atgcgccggt atttttccgg aaatcaccgc caatcagtag aatttctggt
    15901 ccatctgctt gagcaagaat atcaaaaact ggataattat ttgtaatgat ccgaattttc
    15961 ttgttcagta aatgcgtcgc caatagttcg attgttgtac ctggtcctaa gaaaatcgtt
    16021 tctccctcgt gaatcaagga ggctgctgtg gcagcaattt cttttttttc ttgaatctgc
    16081 agagtggctt tttcagtgtg agaaagttct tgatctaaag tgtaggaaag actttgagcc
    16141 cctccatgga cacgcagcag cttgcccgat ttttctaatt catctaaatc tctgcgaacg
    16201 gtcatatcag aaacatctaa ttgattgatg atttcattaa cggtaaggat tcctttttta
    16261 ttgaccattt ctacgatagt aagcaatctt tcttttttca acattgtctt cacctctaat
    16321 ttaaggataa tctatcatat aaacggacaa cagtcaatct aattcgaaca aaagtaaaca
    16381 tttagggtgg tttttatgtt tgttacaata gattttattg ctgttttctg gtttttatga
    16441 ttatttgtgt ttgtttttgt tgacaaatgt tttggatgcg tttacaatgc aatcagttag
    16501 aaagagcgaa aactgattat agatttgagg agagatgtac atgcttaaat taccagaaga
    16561 ttttattttt ggcggagcca cagcagccta tcaggttgaa ggagccacta aggaaggtgg
    16621 caaaggcgct gttgcttggg atgacttttt agaggaacaa gggcgattca gtcccgatcc
    16681 ggcaagtgat ttttatcatc aatatgcaaa ggacattgaa ttgtgtgaac gttttggtgt
    16741 aaatggtcta agattatcca ttgcttggag ccgcattttt cctgatggag ctgggaaacc
    16801 aaatccagaa ggaatcgctt tttatcatcg tgtgttcgaa gaatgtaaga aaagaaacgt
    16861 aacgccgttt gtgacgttgc atcattttga tacccctaaa cgtttgtttg accatggaga
    16921 ttttttgaat cgagaaacaa tagaagcatt tgtttcctat gccatttttt gttttcatga
    16981 attcaaagaa gtcaaggtgt ggagtacctt taatgaaatc tatccagttg caaccaatca
    17041 atatttgtta ggtgttttcc caccaggaat caaatatgat tttacaaaga tcgtcgcctg
    17101 tcttcataat atgatggttg ctcatgcccg tgtagtgaac tatttcaaag aaaacgaact
    17161 tccgggtgaa attggtgtag ttcactcatt agagacgaaa tatgcggcaa cagacgcgcc
    17221 ggaagataag catgctgcct ttttagacga tgcattgtcg atccgtttct tattagatgc
    17281 tacctattta ggctattatt caactgaaac cttgactgct cttgatgaga tatgtgaagc
    17341 aaatcaggcc agctatcatt ttcctgaaga agatttcgtt gaattgaaaa aagcatccac
    17401 gcgtaatgac tacttaggga tcaatcacta ccaatgccat tttgtcaaag cttacgatgg
    17461 agaaaatgca atccatcata acggtacggg tgaaaaaggg acctctgttt ataaagtgaa
    17521 gggaatcgga gaacgtattt ataaagaagg aattcctcga actgattggg actggctgat
    17581 ttatccagaa ggtctatatg acttgttgct gcgaattaaa agcgattacc cacattacaa
    17641 caaaatctat atcactgaga acgggatggg gtataaagat cagtttgaag atggcattat
    17701 tatggatcag ccgagaatcg actatttgag agtctatttg gaatccttaa gcaaggcgat
    17761 aacagccggg gtaaatgtga aggggtattt tttgtggtcg cttatggatc ttttctcatg
    17821 gacaaatggg tataacaaaa ggtatggctt gttttatgtt gattttgaaa cccagaagcg
    17881 ttacccaaaa gaatcagctt actggtacaa gttggtcagt gaaaccaaaa cgattattta
    17941 ataagtgata atggaggaag aaaagatgaa taaagaggaa ttacaaatgc ttggttttga
    18001 gatcgttgca tattcaggcg acgctcgcag tacattattg aaattattga aagaagtaag
    18061 acaaggaaac tttgaacatg ttgattcggc gttgaaagaa gccgacgaaa atttaaccct
    18121 tgcacataat tcgcaaacaa aaattttagc ggaagaagca tctgggaaag agatggagat
    18181 gggattcatt tttatccatg ggcaggatca tttaatgacc actttattgc tgagagatct
    18241 gattcaagac tttattgtac tgtacagaca gaatcaagga taggagaaga acgctatgga
    18301 tggaatcatt aaacaaatcg aaaaagggaa accatttttt gaaaaaatct cacgtaatag
    18361 ttatttagga gccgttcgtg acggattttt aacagcgatg ccagctattt tattttcaag
    18421 cattttcatt ttgattgctg cggttcctga gatttttggt taccaatggc ctgaatctgt
    18481 ctccacatgg ctatggaaag tctataatta ttcgatgggt gttgtagggt tactcgtttc
    18541 agcaaccact gctcgctgct tggctgattc gatgaatcgt cgcttaccga aaaacaaagt
    18601 aatcaacacc acctctgtga tgctggcctc tgtggtaagt ttcctgttgt taagcgtctc
    18661 tccgattgaa ggtggatttt caacggaata tatggggaca aagggactac tggcttcctt
    18721 tgtcgctgca tttttaacag ttaatttata taagttttgt gtgctgaaag atattaccat
    18781 taaaatgcct aaagaagttc ctggaacgat ttcgcaaacc ttccgagata tttttccttt
    18841 ctctttttca gtatttgcgg ccgttttaat tgatttaggc attcgacaaa tcttccaaat
    18901 ctcatttgca gaagcaatca ttacggtgct gcagccgcta tttacagcag ctgacgggta
    18961 cttaggaatc gctattatct ggggagcgat ggcgctgttt tggtttgtcg gtgttcatgg
    19021 accgtcgatc gtggagccgg caattgcggc aattatctat gccaatgtgg aaaccaatct
    19081 gcaattgttc aattccggtg aacaagcttc taaagtactg acggtgggtt tagggaattt
    19141 tgtaggaact atgggaggaa ctggggcaac attggttgtt ccttttatct tcctactctt
    19201 tgctaagtct aagcagttga aggcagttgg gaaagcttcc tttattccag taagtttcgc
    19261 tgtcaacgaa ccattgctat ttgcggcacc catgattttg aacccttatt tttttattcc
    19321 atttttgtta acacctgttg taaacgtttg cctgttcaag ttttttgtcg atgtattggg
    19381 catgaacagc tttatgtacg ttttgccttg ggcaactcca gcaccgatcg gtttggttct
    19441 aggaacgggc atgggtatct tagcttttgt tctagcagcg gttctaatcg tcgttgactt
    19501 cctgatctat ctaccatttt gcaaagcata cgatgctgtc ttagtagaga aagaagcaca
    19561 aaaagcagag gtgatcgcaa ccgaggaagg tgcttctgcc gatgtacgtg aggaagtacc
    19621 tgtggcagcg agtcaagtta tcagcgatgc aaataaagaa atcaatgtgt tggtactttg
    19681 tgctggcgca gggacaagtg cgatgttagc taatgcttta aaaaaaggtg catctgaatc
    19741 gaaagcaccg atcagtgcaa tggcgggtgc ttatggttct cattacgaaa tgctgcagga
    19801 ttttgatatg gtggtcttag caccgcaagt caattcttac tatgatgata tcaaagaaga
    19861 caccgatcgc ttaggaatca agctggttgc gacaaaagga gcagaatata tcggattaac
    19921 gagagatcct gaaaaagctg ttcagtttat tctgaaccaa tttcaataaa ggaggagcca
    19981 taatgaaaat gggaatttgg ttgcgtaaag ctgttttatt tgcagctgtg ttaggggctt
    20041 tgggggctgt ttcaaaaact gttgaagcat ctgccagcga atttattcgc ggagcagata
    20101 tttcgatcct gtcagatatg gaaaaatctg gtgcaaaata ttatgaagac gatatttcaa
    20161 aagatgcctt agagatttta aaaaataatg gtgtcaacta tgttcgactg cgtttgtggc
    20221 aggatcctta taatagtaat ggagaaagtt atggagcagg gacaaatgat ctggaaacga
    20281 cgattgacct tgcaaaacga gcaaaaaatc tgggacttaa ggtgctgctg gattttcact
    20341 acagtgattt ttgggttgat cctgggaaac agaatcttcc taaagcatgg caaggtctga
    20401 cctttgaaga aatgaatacg gcattgtacg actatacaaa gaacgttctg tcggaaatga
    20461 agcagctgga cgtttatcct gatatggttc agatcggcaa tgaactgaat agcggaatgc
    20521 tctggcctta tggcaagtct tggggagaag gcgggggaga atttgatcga ttggctgctt
    20581 ttttgaagtc tggtatccaa gctgttcgag acactcaacc taaaacaact ccagtaatgc
    20641 tccacttagc tgatggtggc gatacaggag catttacttg gtggtttgat gaaatcacga
    20701 gtcgaggtgt gtcatttgat ttaattggag tgtcgtatta tccgtattgg cacggttcaa
    20761 tgaatgaact tcaaaccaat atgaatgcga tcagccaacg gtatggtaaa gaagtgatcg
    20821 tagttgaaac agcttatggg catacaacag cgaatgccga tactatgcca aatgcttttg
    20881 gtgaagccga agcagctgcg gggggatatg aaccaacacc gacaggacaa gcagagtatt
    20941 tgctggatct tgccgatcgg attcaggcag taccgaataa tcgcggggcg ggcttcttct
    21001 attgggaacc tctttggtac aacggaaatg tgagttgggc aacacaagca ggtatgaatt
    21061 atctcagtgt tcaatccact atgggaaacg aatgggacaa ccaagcgatg tttgattttg
    21121 ctggaaacgc cttgccaagc ttgcgtgcgt ttaaacaagc aggggcgcaa ataaatctag
    21181 ttaaaaatgc aagtttcgaa agcgatggac caacgaccag tccttccaat tggcaaacct
    21241 ggtttcaaca aggaaccgct aagggtacga tcaaaacgga agcgggcact ttttttgggg
    21301 actacaaact aactttttgg caggatcaag catacgaggc atcagcttat caaaatgtta
    21361 gtggcataag caaaggaact tacacattga gtgcctatgt aatgtctggg ggagagtttg
    21421 atactaatca gttatatatc aaaaattatg gtggagcgga actaaatact gcggttgtta
    21481 aaacaagtga atggacaaaa attacaatca aagacattcc aattacaaca ggtgtttgtg
    21541 aaattggggt ttatagcaag gcttcaggaa atgcttggac gaacgttgat tttgtttcat
    21601 tggtgaaaca atagactgcg gatcgtttcg attcaagatc ggaggttccc ttcagccaca
    21661 tcgttagcgt agaaggcagg aatcgaacct gcacctcgcc tttgccaaaa ataaccagaa
    21721 acgatttcca gtcattttta ccaaaacgag taaagcacca gacttcatct acactttgta
    21781 ttatgctaaa gagagttcaa cgatttcgta agaagcaatg aaaaaattgc aaaaagttgt
    21841 gcagataaca taaagtttgt ttaagaaagg gtaaccttag aattccgtcg tgaatatgat
    21901 ttgagcactt agatggatag aaaaaagttc tcggaaaaca gttcgttttt cgagaacttc
    21961 tttttatgat aagaatgaaa caaattaact tatattatag tctcttttgg tagggaacca
    22021 tcttattttt aatgaaaatc aagcatttta gctattgaag aatgttgatt tatagggatt
    22081 ccccctccat attatgttgg ctctttattc ccactctgtt ttcaacgtaa agtgtataaa
    22141 acagaatgcg atactatcaa gggagttaaa acaatataca gaacagtaaa acactataaa
    22201 taaaaataaa agtgcaataa aagtgcaatt tttgcaccta atcatactct gtttatatat
    22261 gaaaactaga ttctgaatcc aatatttggt taacgagagg cgttcgaatt tgaacgtctt
    22321 ttatatatta ctttcaaata aatatgaggt agaatactat ctattttctt tttaacccaa
    22381 ttgttataat ggagagtgta aaatattttg tgtaaataga aaaaaggaag tcccttctgt
    22441 agaatagagt taccacaaca cattcacaga agagaggact tccctatgaa cgattttact
    22501 acagaaattc taaagactct agcgaacaaa ggcgatttga atgaattatt ccgtgtccat
    22561 ttggagaaag cagtcaatac gcttctcaaa acggagttaa cggctttcct agattacgaa
    22621 aagtatgatc gcattggttt taacacgggt aattctcgta acggctccta tgaccgtacg
    22681 gtcaagaccg agtatgggga acttcatctc cagattccgc gcgaccgcaa tggtgagttc
    22741 aagcaacaga ctgttcctgc ttatagacgg acgaatgaca cgttagagga gaccgtcatt
    22801 cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag acttgattga gaaaatgtat
    22861 gggcattact acacgcccca aaccatgtcc aatataacaa aatcatttac agaagaggta
    22921 acggcgttta atgggcggga gcttcatgac cgttatgctg ctatttatat ggacgcaacg
    22981 tatattccgt taaagcggaa aaccgtcgcc aaggaagcta ttcatatcgc agttggcatt
    23041 cgcccggacg gatcaaagga agtattgagc tatgcgattg caccgactga atccatcacg
    23101 atttgggagg aaattttatt ggaccttcaa gagcgcggtt tgaaaaatgt cctcctgttc
    23161 atcacggatg gcttaaaggg gatggtagga gcgattagtc ggttctatcc caaagctcgt
    23221 tttcaacatt gttgtgtaca cgtttcccgt aatatcagtc ataaagtgcg tgtcgatgat
    23281 cgtaaggaag tctgtgatga ttttaaaatg gtgtatcaag cctcatctaa agaggtggcg
    23341 ttggaagcac gtggtacttt tgcggaaaaa tggaaaacca gctatccaaa agtggttgaa
    23401 tcgattcttt cgaacgatca cttgctcact ttctatgatt tccccttggc catacgcaag
    23461 agtatttatt ctacgaactt gattgaatcc tttaataaac aaatcaagaa atacagccac
    23521 cgcaaggaac agttccaaaa tgaagagtcg atggaacgtt tcttagtctc gtcttttgat
    23581 acttacaacc aaaaattcct aggtcgcagt cataaaggtt tccaacaggc cgaaggcgag
    23641 ctcgaacaaa tgctaagcca actgattgag aattagaaac aatctgcagt agggaagaat
    23701 catttacaca aaattattga cgctccctta taatgagaac ttttactaac ataatttatg
    23761 ataaacttac tatataatca tattaattaa atagttagga gatctgctta tgtttatatc
    23821 tactcttcaa attcgaaatt ttagaaatta tgaatccgaa atctttcgat tcgataatga
    23881 gacaaatact attataggtg agaatgattc agggaaaacc aatgctctta ctgcattgag
    23941 aatcttatta gatgatagtt attactatag tagtaaaaca ctaaaagaat cagatttttt
    24001 tcatggaatt caaaatgggt ggcaagggca ttggattatt atctctgcga catttgaagg
    24061 aatcagcgaa gaagaatttg acaatgagat ttgtgcttcc atttctttaa attctgaaag
    24121 tcaaacaatc ttagaagaat tgatttcaaa tgcagataaa ggtgtaggta gtatttctct
    24181 atttattaga cctaacaaag ctattagaaa gcaattattt gatatttcca ctgaattaga
    24241 ctctcatcaa tttaacgaat ttagaaattc tattcgtcta tccgactatg aattttacta
    24301 cacatctaaa tcaaatctaa atttctgtat caatgaaaat tatgataaat tggtaggaag
    24361 actcaatgac aatatagcta gtaatcctga agaagacgat gaagctctgt tgggctcaag
    24421 aattgacatg tcagatattt tcaagcatat ttcggttgtt tatgttgacg ctcttcgaga
    24481 tgttcttaga gaaatgaaaa acaatcgaaa cccagtcaaa agaatcatgg aaacaattga
    24541 atcaaaaata tcatcagata atgttgagtc attaaaaaca attatacagc aattaaatga
    24601 gactattacc agtgttcctg aaattaggaa aattggcgaa aatattaatc ggcaactaaa
    24661 tagcataatt ggttcagtgt attctcctaa tcttcttctt tcctcaacta tgtcagatga
    24721 tatgggttct ttagcaaaat ttctttcaat gaagccagaa caaaatatcg acttagactt
    24781 actagggtta ggacatctaa atatgattta tttagctttg aaaatagtcg aatttgaagc
    24841 gtgtcgttca agagaactac ttaatattat gttgattgaa gaaccagaag ctcatattca
    24901 tcatcatatt caaaaaactc tgttcgaagg attaaatctt caaaaaaact atactcagat
    24961 tctcatgacc acacattctg tacatttagc tgaagtgtct gaaatatctc ggatgaatgt
    25021 attaaaatcg tttaatggca aatctatcgt tatgaatcca gcaaataatc tgaactcatt
    25081 tgctaaagaa aagttaaaaa agaacaatct aaatctaata aacgcagtag agagatattt
    25141 agatgttaaa agaaatggat tgcttttttc taaaggtgtt atcttagttg aaggagatgc
    25201 tgaagaaata cttattcctc aaatagtaaa aaaattattt ggtataaact tagacgaact
    25261 tggaataggt ttgattaatg ttggtagtac ttcgtttgaa tatatcgcta gcttgtttga
    25321 cgatgataga atacagagaa agtgtgctat tgtaactgat ttagatgaac aggcaatccc
    25381 atcagaacat cgattgtata aaagcactgc acaagagaaa ggtcaagcaa gaaaagaaaa
    25441 attatcctcc ctttttaatg aaaacaattg ggttaattgt ttttatgcta atacaacatt
    25501 tgaaattgag tttttagatg tgaataaaga aaatattcta acttatgtca gtccaattat
    25561 agatgacgca ttcaaagata aaaaaacaat aactgcttat aaagaagaaa tacaa
//
LOCUS       DACGLH010000032        24271 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.034, whole genome shotgun sequence.
ACCESSION   DACGLH010000032 DACGLH010000000
VERSION     DACGLH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.034"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..572
                     /locus_tag="IXT21_001503"
     CDS             <1..572
                     /locus_tag="IXT21_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4868911.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IXT21_001504"
     CDS             576..1217
                     /locus_tag="IXT21_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868912.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IXT21_001505"
     CDS             1214..2131
                     /locus_tag="IXT21_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4868913.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IXT21_001506"
     CDS             2128..2799
                     /locus_tag="IXT21_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4868914.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IXT21_001507"
     CDS             complement(3340..3828)
                     /locus_tag="IXT21_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ4868915.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IXT21_001508"
     CDS             complement(4029..4295)
                     /locus_tag="IXT21_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ4868916.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IXT21_001509"
     CDS             complement(4295..4558)
                     /locus_tag="IXT21_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4868917.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IXT21_001510"
     CDS             complement(4551..4823)
                     /locus_tag="IXT21_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868918.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IXT21_001511"
     CDS             5034..5219
                     /locus_tag="IXT21_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4868919.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IXT21_001512"
     CDS             complement(5361..7184)
                     /locus_tag="IXT21_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ4868920.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IXT21_001513"
     CDS             complement(7782..10487)
                     /locus_tag="IXT21_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ4868921.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IXT21_001514"
     CDS             complement(10793..11740)
                     /locus_tag="IXT21_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ4868922.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IXT21_001515"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IXT21_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ4868923.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IXT21_001516"
     CDS             complement(12375..12737)
                     /locus_tag="IXT21_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4868924.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IXT21_001517"
     CDS             complement(12748..13869)
                     /locus_tag="IXT21_001517"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ4868925.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IXT21_001518"
     CDS             complement(13885..14235)
                     /locus_tag="IXT21_001518"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ4868926.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IXT21_001519"
     CDS             complement(14365..15876)
                     /locus_tag="IXT21_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4868927.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IXT21_001520"
     CDS             complement(16198..17550)
                     /locus_tag="IXT21_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ4868928.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IXT21_001521"
     CDS             complement(17703..18779)
                     /locus_tag="IXT21_001521"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ4868929.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IXT21_001522"
     CDS             18975..20720
                     /locus_tag="IXT21_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ4868930.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IXT21_001523"
     CDS             complement(21000..21437)
                     /locus_tag="IXT21_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ4868931.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IXT21_001524"
     CDS             complement(21421..22794)
                     /locus_tag="IXT21_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868932.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IXT21_001525"
     CDS             complement(23116..23604)
                     /locus_tag="IXT21_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4868933.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IXT21_001526"
     CDS             complement(23626..24228)
                     /locus_tag="IXT21_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ4868934.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4488 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DACGLH010000033        23825 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.035, whole genome shotgun sequence.
ACCESSION   DACGLH010000033 DACGLH010000000
VERSION     DACGLH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.035"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(53..787)
                     /locus_tag="IXT21_001527"
     CDS             complement(53..787)
                     /locus_tag="IXT21_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ4868935.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IXT21_001528"
     CDS             1114..1524
                     /locus_tag="IXT21_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ4868936.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IXT21_001529"
     CDS             1517..2218
                     /locus_tag="IXT21_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ4868937.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IXT21_001530"
     CDS             2218..3804
                     /locus_tag="IXT21_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4868938.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IXT21_001531"
     CDS             3823..4044
                     /locus_tag="IXT21_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ4868939.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IXT21_001532"
     CDS             4041..4802
                     /locus_tag="IXT21_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ4868940.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IXT21_001533"
     CDS             5182..5691
                     /locus_tag="IXT21_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ4868941.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IXT21_001534"
     CDS             complement(5766..6305)
                     /locus_tag="IXT21_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ4868942.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IXT21_001535"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IXT21_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ4868943.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IXT21_001536"
     CDS             7372..8169
                     /locus_tag="IXT21_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ4868944.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IXT21_001537"
     CDS             8197..8832
                     /locus_tag="IXT21_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ4868945.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IXT21_001538"
     CDS             8852..9625
                     /locus_tag="IXT21_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4868946.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IXT21_001539"
     CDS             10033..10830
                     /locus_tag="IXT21_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ4868947.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IXT21_001540"
     CDS             10808..11221
                     /locus_tag="IXT21_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868948.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IXT21_001541"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IXT21_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ4868949.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IXT21_001542"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IXT21_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ4868950.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IXT21_001543"
     CDS             15998..16558
                     /locus_tag="IXT21_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ4868951.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IXT21_001544"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IXT21_001544"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ4868952.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IXT21_001545"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IXT21_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ4868953.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEELTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IXT21_001546"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IXT21_001546"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ4868954.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IXT21_001547"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IXT21_001547"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ4868955.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IXT21_001548"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IXT21_001548"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ4868956.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IXT21_001549"
     CDS             21905..22483
                     /locus_tag="IXT21_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ4868957.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IXT21_001550"
     CDS             22535..23017
                     /locus_tag="IXT21_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ4868958.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IXT21_001551"
     CDS             23134..23508
                     /locus_tag="IXT21_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868959.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4287 c         5114 g         6643 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaatcgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaac taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaag
//
LOCUS       DACGLH010000034        22873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.036, whole genome shotgun sequence.
ACCESSION   DACGLH010000034 DACGLH010000000
VERSION     DACGLH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.036"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(105..629)
                     /locus_tag="IXT21_001552"
     CDS             complement(105..629)
                     /locus_tag="IXT21_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4868960.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXT21_001553"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXT21_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ4868961.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXT21_001554"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXT21_001554"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ4868962.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IXT21_001555"
     CDS             complement(2351..3031)
                     /locus_tag="IXT21_001555"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ4868963.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IXT21_001556"
     CDS             3157..4026
                     /locus_tag="IXT21_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4868964.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IXT21_001557"
     CDS             4052..4567
                     /locus_tag="IXT21_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868965.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IXT21_001558"
     CDS             complement(4614..5177)
                     /locus_tag="IXT21_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4868966.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IXT21_001559"
     CDS             complement(5232..5411)
                     /locus_tag="IXT21_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868967.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IXT21_001560"
     CDS             complement(5488..5997)
                     /locus_tag="IXT21_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868968.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IXT21_001561"
     CDS             complement(6093..6473)
                     /locus_tag="IXT21_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ4868969.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IXT21_001562"
     CDS             6803..7552
                     /locus_tag="IXT21_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ4868970.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IXT21_001563"
     CDS             complement(7736..9013)
                     /locus_tag="IXT21_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868971.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXT21_001564"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXT21_001564"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ4868972.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IXT21_001565"
     CDS             complement(9935..10879)
                     /locus_tag="IXT21_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ4868973.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IXT21_001566"
     CDS             complement(10881..11567)
                     /locus_tag="IXT21_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ4868974.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IXT21_001567"
     CDS             12041..12361
                     /locus_tag="IXT21_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ4868975.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IXT21_001568"
     CDS             12515..13420
                     /locus_tag="IXT21_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4868976.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IXT21_001569"
     CDS             13417..14190
                     /locus_tag="IXT21_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4868977.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IXT21_001570"
     CDS             complement(14371..15795)
                     /locus_tag="IXT21_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4868978.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IXT21_001571"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IXT21_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ4868979.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IXT21_001572"
     CDS             complement(16517..18499)
                     /locus_tag="IXT21_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4868980.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXT21_001573"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXT21_001573"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ4868981.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IXT21_001574"
     CDS             complement(19412..20473)
                     /locus_tag="IXT21_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ4868982.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IXT21_001575"
     CDS             complement(20684..21211)
                     /locus_tag="IXT21_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4868983.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="lsa"
                     /locus_tag="IXT21_001576"
     CDS             complement(21278..22780)
                     /gene="lsa"
                     /locus_tag="IXT21_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4868984.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
BASE COUNT         6557 a         4853 c         4042 g         7421 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacgatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc ttttaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gtgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tatcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgcac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctgc gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacat
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatgac aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcgtacata actaatgctc agatgttttg
    21661 gctgggattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atc
//
LOCUS       DACGLH010000035        22637 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.037, whole genome shotgun sequence.
ACCESSION   DACGLH010000035 DACGLH010000000
VERSION     DACGLH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.037"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            9..851
                     /locus_tag="IXT21_001577"
     CDS             9..851
                     /locus_tag="IXT21_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ4868985.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            848..1798
                     /locus_tag="IXT21_001578"
     CDS             848..1798
                     /locus_tag="IXT21_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ4868986.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1813..2046
                     /locus_tag="IXT21_001579"
     CDS             1813..2046
                     /locus_tag="IXT21_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ4868987.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2377..2961
                     /locus_tag="IXT21_001580"
     CDS             2377..2961
                     /locus_tag="IXT21_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4868988.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3092..3760
                     /locus_tag="IXT21_001581"
     CDS             3092..3760
                     /locus_tag="IXT21_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ4868989.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3762..5186
                     /locus_tag="IXT21_001582"
     CDS             3762..5186
                     /locus_tag="IXT21_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4868990.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5204..5773
                     /locus_tag="IXT21_001583"
     CDS             5204..5773
                     /locus_tag="IXT21_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ4868991.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5883..7637
                     /locus_tag="IXT21_001584"
     CDS             5883..7637
                     /locus_tag="IXT21_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868992.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7591..9363
                     /locus_tag="IXT21_001585"
     CDS             7591..9363
                     /locus_tag="IXT21_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4868993.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9886..10155
                     /gene="rpsN"
                     /locus_tag="IXT21_001586"
     CDS             9886..10155
                     /gene="rpsN"
                     /locus_tag="IXT21_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ4868994.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10170..10349
                     /gene="rpmF"
                     /locus_tag="IXT21_001587"
     CDS             10170..10349
                     /gene="rpmF"
                     /locus_tag="IXT21_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ4868995.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10381..10758
                     /locus_tag="IXT21_001588"
     CDS             10381..10758
                     /locus_tag="IXT21_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4868996.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10779..10928
                     /gene="rpmG"
                     /locus_tag="IXT21_001589"
     CDS             10779..10928
                     /gene="rpmG"
                     /locus_tag="IXT21_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ4868997.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10952..11935
                     /locus_tag="IXT21_001590"
     CDS             10952..11935
                     /locus_tag="IXT21_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4868998.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11965..12087
                     /locus_tag="IXT21_001591"
     CDS             11965..12087
                     /locus_tag="IXT21_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ4868999.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12105..13628
                     /locus_tag="IXT21_001592"
     CDS             12105..13628
                     /locus_tag="IXT21_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ4869000.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13652..13891
                     /locus_tag="IXT21_001593"
     CDS             13652..13891
                     /locus_tag="IXT21_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869001.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14084..14545)
                     /locus_tag="IXT21_001594"
     CDS             complement(14084..14545)
                     /locus_tag="IXT21_001594"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4869002.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14740..14958)
                     /locus_tag="IXT21_001595"
     CDS             complement(14740..14958)
                     /locus_tag="IXT21_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4869003.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15076..16692
                     /locus_tag="IXT21_001596"
     CDS             15076..16692
                     /locus_tag="IXT21_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4869004.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16933..18273)
                     /locus_tag="IXT21_001597"
                     /pseudo
     CDS             complement(16933..18273)
                     /locus_tag="IXT21_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18549..18704)
                     /locus_tag="IXT21_001598"
     CDS             complement(18549..18704)
                     /locus_tag="IXT21_001598"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ4869005.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(19000..19332)
                     /locus_tag="IXT21_001599"
     CDS             complement(19000..19332)
                     /locus_tag="IXT21_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4869006.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19846..20037)
                     /locus_tag="IXT21_001600"
     CDS             complement(19846..20037)
                     /locus_tag="IXT21_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4869007.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20131..20517)
                     /locus_tag="IXT21_001601"
     CDS             complement(20131..20517)
                     /locus_tag="IXT21_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4869008.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21211..22416
                     /locus_tag="IXT21_001602"
     CDS             21211..22416
                     /locus_tag="IXT21_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ4869009.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22433..>22637
                     /locus_tag="IXT21_001603"
     CDS             22433..>22637
                     /locus_tag="IXT21_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869010.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESIS"
BASE COUNT         7732 a         3231 c         4221 g         7453 t
ORIGIN      
        1 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
       61 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
      121 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
      181 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
      241 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
      301 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
      361 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
      421 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
      481 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
      541 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
      601 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
      661 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
      721 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
      781 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
      841 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
      901 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
      961 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     1021 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     1081 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     1141 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     1201 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     1261 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     1321 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     1381 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     1441 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     1501 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     1561 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     1621 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     1681 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     1741 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     1801 tggagggtag gtatggtaca aattaaaacg actactatac taatttattt actagcaatt
     1861 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     1921 gttagtccag catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat
     1981 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt
     2041 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     2101 gacttaccct aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag
     2161 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     2221 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     2281 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     2341 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     2401 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     2461 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     2521 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     2581 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     2641 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     2701 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt
     2761 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     2821 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     2881 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     2941 tggattgaat tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg
     3001 ctttttgata agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa
     3061 cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat
     3121 tactttaaat attttttttc cggtcattat ttttttagga aagggaaata tatttcaagt
     3181 ggggtgtttc attattgcta cagttttagt attgatgttt cgtaaaataa agacatgtat
     3241 ttatttcatc ttgatttatt cagttctgtt tttagtaatg aaaattattg atgctatcaa
     3301 gatagagttt tttactatat tttttgggac atccttatat attgtatttc gaatgttgcc
     3361 ggtcgtaatg atctcatggt tgttggttag tgtctatact agcaatgaat tattatcctc
     3421 tttagaaaaa atacatttac cgaaaaaaat aatgttaagt attacagtag tgatcagatt
     3481 ctttccgact tatcgttctg aaataagaat gattaaagaa tctttaaaaa tgagaaacat
     3541 tcgcttgtct ttttttcagc ctctgcaata tttggaattt ctgttagttc ccgttcttat
     3601 acgtgcaact atgattgctg aagaaatgac cgctaatgca ataacaaagg ggatagaatc
     3661 tcctgtgaaa agaagttctt actataaaac aaacatgagc ttattagact atatcatcct
     3721 gttttccgtt actatttttt tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat
     3781 ttaacaatgt ttctttttca tataaggatg ttaaggaaca agcgcttgtt gaccttaatt
     3841 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
     3901 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
     3961 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
     4021 ttggctacgt aagtcaaaat cctagaggac aattctttac aacaaataca acgagtgagt
     4081 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
     4141 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
     4201 gtggtgaacg gcagaaaatt tctattggat gtagtttatc gctaaactct aaattaatta
     4261 tattggatga accgtcttca aatttggact tcaaagcgac taagaaactt gctgacttac
     4321 tttgtgattt aaaaaataaa ggatttacta ttgttatagc tgaacatcgt ttttactatt
     4381 tgaaagaatt aattgatcaa gttctatttg ttaaaaatag aactgtaaaa actacaacta
     4441 ttcagttgtt aaaaactgaa aacaataacg cattaaggaa atttgacatt tttaatagtt
     4501 cgattgaagt tccctatatc gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta
     4561 cttatgaaaa tattctaaaa gacataaatt ttgatgtcaa agttggagat gttattggtt
     4621 tggtagggaa aaacggtgtg gggaaaacaa ccttactaag aatactcatg aagattattc
     4681 gccctaccaa aggaaagata atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa
     4741 tgcaagaaat ggattaccaa tttttcacag aatcagtgag gagcgagtta gccctgggga
     4801 atgaaatagt tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa
     4861 tgaaagatca attaccgttt gatttatccg gtggagaaaa acaaagatta ttggtttcta
     4921 tagcttcatt gtcaaaaact aatttatttc tatttgatga acctacaagt gggttggatt
     4981 atatcaatat ggaaaggatt gctgacctta ttaaagactt aaaagaaaag ggtgcagtag
     5041 ttatagccac tcatgatccg gaatttcttt ataaaacatg caatagaatt atttatttaa
     5101 aaaatggtgg aataaaaaaa gatataaaac ttagagtaga agattcaact ttgatagaac
     5161 agatttttaa tgacataacg aaatagaatt gaggagaata tatatgaaaa caaaagatta
     5221 tatttttatt ggtttagcta cagttatttc cctagtaatt tacttttttt caataatggt
     5281 tagtagtatt ggtggggcgt ttggtcacag catatcccca gggatttttg gattattatc
     5341 tggaatcata tttgtttata ttagctataa ctaccctaga aaaggaattt ttactattta
     5401 cacaattgtt ttattgttat tttttacgtt aatgggcggg gcgtacttac cttggtttat
     5461 cagttcgatt agtatggcga tcttagctga tattattcta tctgtatttg gatatgatcg
     5521 agcaattcct caagttgtgt cttgggttct aatgcaacta ggcagtgctg ccggacagtg
     5581 gattcccata tggttcttta cagatcgttt tcgtcaagac tggatagata gaggacaatc
     5641 tgctgcaaca atggatgcaa tgatccacta cgcggtgggt atatggggaa ttatttcagt
     5701 gttagttgta gcaagtttat ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa
     5761 atacaaaaaa tagaacgtag tttaccttct atctgtttgt acatctctaa tttacgctat
     5821 tacggaaagc attaacagtt gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa
     5881 aaatgaaagt atacaaaaaa ttatttagtt atgtccctaa aatgaaatac tacggatggt
     5941 tagccacttt attttctgat ttttctgtcg tattaacagt ctatggatat tacagtattt
     6001 acaaatttct tcagtctcta attattgtag gagatgaata tttagctaag agttatgcgg
     6061 ttcaaactgt tgcttggtta actttaggag ctttatttta tattttttct ggcttatttt
     6121 ctcatatcct tgggtttcgc ttagagacta atttgagaaa aaagggaatt agcggtttaa
     6181 cagaagcaag ttttcgtttt tttgatttaa attcttcggg atatattcga aagacaattg
     6241 atgacaacgc ggctaaaaca catatggcaa ttgctcactt gattccagat agtgctcaag
     6301 ccatagtaac acctgtttta tcagttgcct taggcttttt cattaacttt aaaattggat
     6361 tggttctaat cgccatgtta gtaattggtg tattcttgtt aaagaaaatg acagagaata
     6421 caaattttat tcaaaaatat caagaatcgt tagatgtgtt aagctctgaa acggtagaat
     6481 atatccgcgg aattcaagtt ataaagatat ttggtgctaa agtaacatca tttaaagcct
     6541 tgaataaagc aattaacgat tatgctaagt ttgcatataa gtattctttg agtgggaaaa
     6601 caccttttgt tctttttcaa tggctatttt ttggcatagt ttgcattttg atagttccta
     6661 taaccttttt tataacttct cttaccactc ccaagatctt agctgttgac ttgttgatgt
     6721 tgtttttttt aagtggaatt atttttgttt ctttcatgag aataatgtat gtaagtatgc
     6781 acttgtttga agcaagatat gcagtcgaaa cactagactc cctttattta gaaatgaagc
     6841 aagaccaact aaaatatggg aacgagacac agattaaaaa ttgtgacatt gcatttgaaa
     6901 atgtcagctt ttcatacaaa gaagagtgtg ttttagaaaa tttcaactta gttttagaac
     6961 aaaaaagaat ttatgcatta gtaggaaaat caggaagcgg aaagagtacg atagctaaac
     7021 taatttcagg tttctacaat gtggataagg gacagattaa aattggtggt aagcctttaa
     7081 atcactattc aaaagacaca attattaaca caattgcttt tgtctttcaa gatacgaaac
     7141 tattcaatca gagtatttac gataacgttt ctatagcaaa tcctcaagcg ccagcaagta
     7201 aagtatttga cgcgctaaga ttagccggtt gcgaatcgat tttagaaaaa tttcccgaaa
     7261 gagagcatac cttaattggt agtaaaggag tttatctgtc aggaggagaa aaacaacgta
     7321 tagctattgc cagagcaatt ttaaaagatg caaagattgt tattatggat gaagccagtg
     7381 catctattga tccagataat gagtatgaac tacaaaaggc ttttcaatat ttgatgaaag
     7441 aaaaaacagt tattatgata gctcatcgat tatccagcat taaatctgta gatgaaattc
     7501 ttgtcttgtc agaaggaaaa atagtagaac gtggaaatca taatgaatta atggctaaac
     7561 atcgagtgta taaacaattg gtcactaatt atgagaatgc aaatgattgg aggcttgaca
     7621 atgaaaaatt tttataaaaa aaaatttgct ttaactgatc agggcgcaga agctttaact
     7681 aaagcttcta tttctagttt ttttgtatac tgcattaata tggtaccagc attcattata
     7741 atgatgctga tagacgaatt agttttagaa aatgcaaagc cccgttggct ttattttgca
     7801 gtatcgtttg ttactttgct tttcatgtat tggttgttag atagggagta tgaaaactta
     7861 tataatagta cttataaaga aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca
     7921 aatttaccat tatcctattt ttcaaaacat aatttatcag atttatctca aactatcatg
     7981 tctgacgttg aaggtattga gcatgcgatg agtcatgcaa tacctaaagc cggcggtatg
     8041 gctctgtttt tcccttttat ttcagtgatg cttttggttg gtaatgtcaa aatgggatta
     8101 gctgttattt tgccaacgtt atttagtttt gtcttaatct tgttatcaaa gaaatcccaa
     8161 acgaaagcca atactaaata ttacgatact ttgagagaaa actcggaaga atttcaagaa
     8221 actattgaat tgcagcaaga gattaatagc tttaatctat ctaaaaaagt tcaagacaga
     8281 cttttcaaaa aaatggaaga gagtgaaagg attcatttaa aggtagaatt aagtactttt
     8341 tcagtcatgg ccttatcctc tattttctca tatgttagtt tagcagtagt cattctagta
     8401 ggtgttcact tactgttaac gggtgaagtg actatactct acgtcgttgg ttacttacta
     8461 gccgcaataa aaataaagga ttccttcgat tctatgaaag aagcagtgct tgaaatattt
     8521 tatttagctc ctaaaataca gcgaattaga gctatgaagg aaaccagtat ccaagaggga
     8581 tcagatagtc ctctgaaatc ttttgacatt gagttgcgag atgtctcttt ctcatatgat
     8641 aacaataccc caattttaga tcatatctct tttacggcta aacaaggaga agtgactgcg
     8701 ttagtaggtg ctagtggttc cgggaaaact agtatcttga aattagtgtc tagactgtat
     8761 gattatgatg aaggctgtat tttaattgat gggtatgata ttaaacgtgt atctccagct
     8821 tctttgttta gcaaaatcgc tattgttttt caagaggtaa ctttgtttaa tacaagtatt
     8881 ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct
     8941 cgtttagcta actgcgaaga ctttattgaa aaactacctg acggttatca tacattggtc
     9001 ggagaaaacg gaagttcact ttctggagga gagcgacaac gcctatccat agcacgggct
     9061 tttttgaaaa atgcgccaat tttaatttta gatgaaatta ctgccagttt agatgcggaa
     9121 aatgaaaaac gtattcagga aagtttaaat cgtttaatac aagataagac agtactaatt
     9181 atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga
     9241 aaactagttg atcaaggtac tcataatgag ttgtatcgaa ggtcagaaat atacaaaaat
     9301 ctcataaaga aaactaaact atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga
     9361 tgatgaaata ggctttaaat taacctacaa tttttgggat atagttataa ttctttttca
     9421 aaaagaataa ttttattaaa cagcatctaa ttttgaatac acgaaagaat cagtatgatt
     9481 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
     9541 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
     9601 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
     9661 agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc
     9721 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
     9781 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
     9841 acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
     9901 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag
     9961 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
    10021 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
    10081 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
    10141 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
    10201 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
    10261 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
    10321 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
    10381 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
    10441 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
    10501 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
    10561 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
    10621 gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat
    10681 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
    10741 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
    10801 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
    10861 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag
    10921 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
    10981 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
    11041 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
    11101 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
    11161 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
    11221 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
    11281 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
    11341 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
    11401 catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
    11461 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
    11521 tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt
    11581 tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt
    11641 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact
    11701 ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
    11761 gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa
    11821 ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt
    11881 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
    11941 agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
    12001 cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa
    12061 cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
    12121 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
    12181 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
    12241 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
    12301 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
    12361 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
    12421 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
    12481 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
    12541 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
    12601 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
    12661 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
    12721 tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta
    12781 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
    12841 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg
    12901 caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc
    12961 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
    13021 aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
    13081 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
    13141 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
    13201 atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag
    13261 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
    13321 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
    13381 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
    13441 gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag
    13501 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata
    13561 attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg
    13621 ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga
    13681 ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg
    13741 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
    13801 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
    13861 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
    13921 cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact
    13981 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
    14041 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
    14101 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
    14161 gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg
    14221 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
    14281 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
    14341 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc
    14401 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac
    14461 ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat
    14521 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
    14581 gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa
    14641 agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga
    14701 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
    14761 atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt
    14821 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
    14881 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag
    14941 atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
    15001 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
    15061 agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca
    15121 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc
    15181 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
    15241 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
    15301 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
    15361 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
    15421 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
    15481 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
    15541 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
    15601 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
    15661 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
    15721 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
    15781 tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
    15841 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
    15901 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
    15961 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt
    16021 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
    16081 aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
    16141 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
    16201 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct
    16261 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
    16321 gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct
    16381 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat
    16441 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
    16501 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
    16561 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
    16621 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac
    16681 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
    16741 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
    16801 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
    16861 ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
    16921 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
    16981 ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg
    17041 gtagttttga aagtttctat agccataggc aattcgtttt agtactttga tatggttatt
    17101 caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg
    17161 gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc
    17221 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg
    17281 gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc
    17341 tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc
    17401 ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc
    17461 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg
    17521 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
    17581 gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg
    17641 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
    17701 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga
    17761 tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg
    17821 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat
    17881 caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc
    17941 aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt
    18001 acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa
    18061 taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg
    18121 atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaagcaaaac atgtttcgtc
    18181 attgatgcgc tctttgcgaa aggcatcctc atgaaaggta agatttaggt ctagaatatc
    18241 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactactttg aatggttttt
    18301 gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa
    18361 gtattggcaa cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct
    18421 tcaaattgtc aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct
    18481 tctaaataat cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg
    18541 attaattctc atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc
    18601 ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa
    18661 gaccgatgct ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt
    18721 tgtaatggac ggacgacaat ctcttttttt atgtgtgaat ctaccatata gtaacgtaga
    18781 atacgagttg ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg
    18841 taagtaatat ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac
    18901 atttttttac catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa
    18961 atcctcttgt gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt
    19021 cgattccttc atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt
    19081 cgtagaacac tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc
    19141 tgctttatca ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata
    19201 ttctgaaacc catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt
    19261 ggtagttact tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata
    19321 tgtttttctc aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta
    19381 tcatagccca acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag
    19441 tttttaacaa ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac
    19501 atagttgaat aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag
    19561 gagaagtcct cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat
    19621 acgtaaataa agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac
    19681 gaattaaaaa acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa
    19741 cgctgtaaaa tcgatacttt ataattataa agaagatagt ttcaaattta ttgttcgaaa
    19801 tttgaaacta tctttttact tatgtcacag actcccttag ttgagttatt ctttaatttc
    19861 gaatttcgag atagctagat atattgcaag catacattct tgataacaat cttgatcata
    19921 ttttccagtt ctccaggatt gcttatgaat caaagagcta aatcttttga gaattgcctc
    19981 aacagcttct tcatttccag cctttgctaa aatgaaatct tcttttaaag aattcataaa
    20041 tctaccttct tcccatggac ttataatcta tttctttggg aaggaggaga acgagcaaag
    20101 aagcatacaa aattagttat tatgcagata ttaaaccagc attttttgaa tttttttgta
    20161 aagtcgatgt tttaaatttg tcgcaccctg tcttgtaatc cccagaacct ttccaatttc
    20221 tttatcagtt ttatcaaaga taaatttttc gataagaaaa atttgttcag gttctttcaa
    20281 caaaggaagg atttttgcta attttatatc ttcaacaagg agagtcacat ttaaaacatt
    20341 aatcggttgt ttaaatttta tagtatcttt agtaaacaaa tatggtggga gttgatcaag
    20401 ttgttcatgt ttttgaattt cttcttgttt tttataataa gtattagcgg tgttcaaaat
    20461 gaccttttca aagaatcgaa tcaactgggc atttacgcgt tcgtcgttat ctgacatatt
    20521 gcacacctcc tccattaaga tttaaaggga aaagaggcaa aaaacacgaa aagaagctaa
    20581 tgcaattatg ctgcgctgcg aataacttct ataagctcac atgcattgat aggactgctg
    20641 atgtttattt tcaaaaatgt ttttttagtt gataatggat agttatcttt atacttagaa
    20701 aagaaaattc atatatcttc ttaaatatca aacatgtcct tatggctaag aaaatgtctt
    20761 gagcttttta cgtataataa atcttttcta aaacctcctt ttggataata ttcttattcg
    20821 tgtatagatt gtatttttta tttcttcccc cttcataatt aacaactaga aaagagttcg
    20881 aatagacacg tatttgaacg aaaattttta ttggattccc aaattggaat atttagaaac
    20941 gaaaaattcc tattatctgt atagtgctta gataataacg gatcataata attattgtct
    21001 ttgaactaga tatatgtaaa tagtattcat catgatgaaa aatgatgagt tctcggtgat
    21061 aaaatgtact ttttacggtg gaatatacat gaaagcgata agtggatatt ttataatgaa
    21121 gtaagttagc ttattttgac agaggaatag tattttagat aacgtataaa atactattta
    21181 atgatgtaga aaggaaagag agaaacaaaa gtgttttcag ataaagaagt cttattaaag
    21241 tctcttcatg tttgtttaaa gttacctatc gtggttttta attccgaatt cagtattata
    21301 aaagagtatc gctcagatag aactacttat tttttttatg attataaaaa gttactaact
    21361 gataatatta aaaaaactaa agatttccat ttctttactg gtgattttaa tgagatgttt
    21421 cttctataca tgtttaaaac cagatattac ttatttggtc catttagagc aaataatatt
    21481 gataaagttt tttttaaact aaaaatgaac aatttgaatg ttgcaatggc tgatagagaa
    21541 cgactctata attcattgca aaatttgact ttgtattctt taggagacat tcgagatatt
    21601 ctgattttag ttcattattt ttttactgga aaaatagaag atctattcca tgaaccgtta
    21661 attgaatata cagaaaatct aagtaaaact atcgagcaaa tcaaaataga taatctgtta
    21721 tcacaaaatt acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat
    21781 gttaaaaatg gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata
    21841 attccaagtg ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt
    21901 gaaaagttag cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt
    21961 agagatgctt tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg
    22021 agggattctg ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt
    22081 tctcccttaa tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact
    22141 gtaaaggaaa tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag
    22201 aatgaaatgc atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa
    22261 ataatgttga aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat
    22321 tcttcacact tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa
    22381 ttaggtttag tggacaattt caacaacatt agctgagagg ttttatttta ttatgagact
    22441 atatcattta cgtagtttgg aggagttgag gaaattggat tttaacaaaa aatttttttg
    22501 ggaaatttct gatttgagtt gtttcgtaga atctgagcgt ctaagtaatg gtaataatag
    22561 tgtgttaaat gaatatgaca aagtagtaat taataaagta aaaagtttat gtgaagaagc
    22621 tagagaatct atcagtc
//
LOCUS       DACGLH010000036        22068 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.038, whole genome shotgun sequence.
ACCESSION   DACGLH010000036 DACGLH010000000
VERSION     DACGLH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.038"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..290
                     /locus_tag="IXT21_001604"
     CDS             <1..290
                     /locus_tag="IXT21_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ4869011.1"
                     /translation="KSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGILIYPEKGEV
                     RAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQQG"
     gene            294..464
                     /locus_tag="IXT21_001605"
     CDS             294..464
                     /locus_tag="IXT21_001605"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869012.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(504..1406)
                     /locus_tag="IXT21_001606"
     CDS             complement(504..1406)
                     /locus_tag="IXT21_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4869013.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            1637..3040
                     /locus_tag="IXT21_001607"
     CDS             1637..3040
                     /locus_tag="IXT21_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ4869014.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            3040..4473
                     /gene="ascB"
                     /locus_tag="IXT21_001608"
     CDS             3040..4473
                     /gene="ascB"
                     /locus_tag="IXT21_001608"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ4869015.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            4618..5544
                     /gene="trxB"
                     /locus_tag="IXT21_001609"
     CDS             4618..5544
                     /gene="trxB"
                     /locus_tag="IXT21_001609"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ4869016.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            5758..7485
                     /locus_tag="IXT21_001610"
     CDS             5758..7485
                     /locus_tag="IXT21_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ4869017.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            7557..7850
                     /locus_tag="IXT21_001611"
     CDS             7557..7850
                     /locus_tag="IXT21_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ4869018.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(7942..8802)
                     /locus_tag="IXT21_001612"
     CDS             complement(7942..8802)
                     /locus_tag="IXT21_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ4869019.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            8894..9322
                     /locus_tag="IXT21_001613"
     CDS             8894..9322
                     /locus_tag="IXT21_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4869020.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            9484..9660
                     /locus_tag="IXT21_001614"
     CDS             9484..9660
                     /locus_tag="IXT21_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ4869021.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            9687..10133
                     /locus_tag="IXT21_001615"
     CDS             9687..10133
                     /locus_tag="IXT21_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ4869022.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(10287..11666)
                     /gene="brnQ"
                     /locus_tag="IXT21_001616"
     CDS             complement(10287..11666)
                     /gene="brnQ"
                     /locus_tag="IXT21_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ4869023.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            12372..13343
                     /locus_tag="IXT21_001617"
     CDS             12372..13343
                     /locus_tag="IXT21_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ4869024.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            13367..15559
                     /locus_tag="IXT21_001618"
     CDS             13367..15559
                     /locus_tag="IXT21_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ4869025.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            15576..16052
                     /gene="ybeY"
                     /locus_tag="IXT21_001619"
     CDS             15576..16052
                     /gene="ybeY"
                     /locus_tag="IXT21_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ4869026.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            16030..16431
                     /locus_tag="IXT21_001620"
     CDS             16030..16431
                     /locus_tag="IXT21_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ4869027.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            16446..17345
                     /locus_tag="IXT21_001621"
     CDS             16446..17345
                     /locus_tag="IXT21_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ4869028.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            17488..18300
                     /gene="recO"
                     /locus_tag="IXT21_001622"
     CDS             17488..18300
                     /gene="recO"
                     /locus_tag="IXT21_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ4869029.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            18684..19601
                     /gene="glyQ"
                     /locus_tag="IXT21_001623"
     CDS             18684..19601
                     /gene="glyQ"
                     /locus_tag="IXT21_001623"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ4869030.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            19603..21678
                     /locus_tag="IXT21_001624"
     CDS             19603..21678
                     /locus_tag="IXT21_001624"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ4869031.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         7384 a         3889 c         4742 g         6053 t
ORIGIN      
        1 aaaaatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct ccactaaatg
       61 cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg atctaccctg
      121 aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gaccctgttc ccgacgaaac
      181 aggcaattgg aatggtttct gaaacagggc tggaattgct gattcatgtc ggattggata
      241 ctgtgcagtt ggaagggaaa tattttgaaa gcttagtgca gcaaggataa agagtgaaac
      301 aaggcgatct gttgctacgc ttcgacaaaa agaaaatcga aaaggaagga tacagtttag
      361 aaacacctgt gatcgtcacc aattacacgg attatttgga tattttggaa aaccaagggg
      421 agacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt aggtgaagaa
      481 tggactaggc ttaactattc tttttattcc acttctttat tttcaaaatt gaaaggctct
      541 ttcatgatgg ctgagctgat agggcgttta tcgaattttt gaccgacaga aattagatgg
      601 actaaattct gttgataatt ttctgcaaaa acacaactat ataacacgtc tagaagataa
      661 caaattgatg tattgatcgt aaaattacca atttttgaat acagtcgctc gcgtgtcgta
      721 agtcgcaaag cggctgtact cattgcagat agcgttgaat caccgatact ggtcaaagct
      781 aaaattggaa catgattttg tttcaatatt tctgcggtac gaaggatcac attgttttca
      841 cccgtataag aaatcagaat cccgcacata tcactcgtgc aattgtacgc ttcatagaat
      901 gattcaccaa aagtggtgac aatcgtgaca tccattttga tgcgcttcat tttcagcatg
      961 aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt ttttgcttga
     1021 agcattagtt gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag tgtatcatga
     1081 atcgtcatct gttctaaaga agccatttta tttgcgatcg tcataatacc gtctgtggtg
     1141 gaaaaaggaa gattcgcatc tacgtcttga aaattccctt caagataggc tgtttcagct
     1201 aaaaaagcat tacgaaaact gacccagcca tcaaaaccca ttttttttgc tacacggatg
     1261 agtgtagagg ggtggacata acatcgctca gcaatcgctt ggatggtcat ttcgccaagt
     1321 gctggagaat cgcttaagag aaactggatc acttcatttt ctgaaggaga aaaggagatt
     1381 tctttgattt tttcacgtaa taacatgtag ataccttctt cttatttttt tacgtaaatt
     1441 ataacttttt tgaaaatgat ttgtaaacgg atttatacta tataacttaa aatagacatt
     1501 ttttgacaag atatttacaa aaatccacta gaaaacagta aattttgtaa atgttactgg
     1561 aaagaaacag atttctattg tttgttttgt ttacaaaaaa gataatgaaa gtgtaaaaaa
     1621 gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg gaggcagcag
     1681 ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac cgatacgaga
     1741 aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtta gtcagatgtc
     1801 tcagcgtatg tgggatgctt ctccgtatga agtcaagatc tatgcaactc tggatcggga
     1861 agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc aattagatgc
     1921 acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag aaacaaatgg
     1981 tgctggcggg atattcaaag cttttcgtac cgttccagtc gttttggcaa tcgttgagga
     2041 catgaagcgt ttatgtccaa atgcttggct gatcaatttc gcgaacccaa gtgggatggt
     2101 gacagaagca gttatcagat atggcaaatg ggataaagtg attggattat gcaatgtgcc
     2161 agtgatggcg atgatgacag aaccagcaat gcttggcaag aaaaaagaag agctgatcta
     2221 caagtttgcc ggtctaaatc atttccattg gcacaaagta gcagataata aaggaaatga
     2281 ccgcacggca gaattgattg atcgtttata cgatgaagag aatggattac cgaaaaatat
     2341 ctttgatatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc cttgtggcta
     2401 ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag aatatcaaac
     2461 gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctatttg aattatacaa
     2521 agatccagaa ttagaccata aacctgaaca attgcaaaaa cgaggtggcg cttattattc
     2581 agacgctgcc tgtgaaacga ttgcttcaat ctatggaaat aaagaaacac agatcgtggt
     2641 atctacaaag aatgatggag ctgttcctga cctgccggca gattgtgtag tagaagtcac
     2701 tgcctatgtt ggcgcacaag gagcgagaaa tgtagcgttt ggttctcttc ctgctgctga
     2761 aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag cagctgtgac
     2821 tggtgattat gggacagctt tacaggcgtt tacgatcaat ccacttatac gatcaggaaa
     2881 gacagcaaaa cggatcatgg atgagttgtt tattgcacac cagcagtatt taccgcaatt
     2941 caaggagaca atcagccgat taaaagaaga aggaatcgaa gttcaagatg aactggcaag
     3001 agaattatca ggtaatacac atataatgga ggaagtataa tgggattcag aaaagatttt
     3061 ttatggggcg gtgctacagc agccaatcaa tgtgagggcg gttatgatga aggtggacgc
     3121 ggactagcaa acgtagatgt tgtgccaatc ggaaaagacc gttttcctgt tatcaccgga
     3181 aagaaaaaaa tgtttgaatt tgatgaggat catttttatc cagcaaaaat tgcaatcgat
     3241 atgtatcatc gttataaaga agatattgct ttgtttgcag aaatggggtt caagacttat
     3301 cgaatgtcca tcgcctggag tcgtattttt ccaatggggg atgagaaaga gccgaatgaa
     3361 gctggcttga aattttatga ggatgccttt aaagaatgcc aaaagcacgg aattgagcca
     3421 cttgtgacga ttacgcattt tgattgtcca atgcatttga tcaaagaata tggtgcgtgg
     3481 cgttccagag aactcgttgg tttttatgaa aatctgtgtc gagtcatctt caatcgctat
     3541 aaaggacttg tcaaatattg gctgacattc aatgagatca atatgatcct tcatgcacca
     3601 ttcatgggcg cagggctgta ttttgaaaaa ggagaaaatg aagaacaaat caaatatcaa
     3661 gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agattgctca tgaagtagat
     3721 ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata cacttgcaat
     3781 cctgaagatg tttgggcttc tagaaaatca gatcgagaaa attatttctt cattgatgtc
     3841 cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggagcgaaa tggcattcag
     3901 atcgaaatgg aaccagggga tgccgcattg ctgaaagaac atactgttga ttttatctct
     3961 ttttcttatt atgcttcccg agtttcgact accgacaacg aactcttgga acagactgca
     4021 ggaaatatct ttgcttctgt caaaaatcca tatctgcaag caagtgaatg ggggtggcaa
     4081 attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta tcaaaaacca
     4141 ttgtttattg tagaaaacgg cttaggtgca gtcgatacgc cagatgaaaa tggttatgtg
     4201 gaagatgatt accgaatcga ttacctggca gcgcatatcc aggcgatgaa ggatgcagta
     4261 gaactagacg gagtcgatct attaggctat acgacttggg gatgtattga tttagtatca
     4321 gctggtaccg gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg tgataaccaa
     4381 ggaaatggca cattagcccg ttcaaagaaa aaatcattca actggtataa aaaagtaatt
     4441 gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag tctattgttt
     4501 ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga acaatagact
     4561 ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa tctacatatg
     4621 tatgatgtaa tcgttattgg cgcaggtcct gctgggatga ctgctgcttt atatgcttca
     4681 cgttcaaact tatccgtatt gatgatcgaa caaggtgccc ctggcggcca aatgaacaat
     4741 acggcagaaa tcgaaaatta tcctggattc gattcgatca tgggccccga tctagcttat
     4801 aagatgtatg aaagtgtctc aagatttggg acagaaaatg cttatggaat cgttcaagat
     4861 atcaaggatc acggcagcca taaagaagtc atctgtgaag ataaaagtta cgaaggaaaa
     4921 acagtcatta tcgcaacagg atgtatccac cgtaaattag gcgtacctgg cgaggaagaa
     4981 tttgctggac gaggcgtttc ttactgtgca gtctgtgatg gtgcgttttt ccgtaacaaa
     5041 cgtctagtag tcatcggggg tggggactca gcagtagaag aagccatcta tttgacacaa
     5101 tttgcttctg aagtagtgat cgtccaccgt agagatgaat tacgtgcaca aaagatcatc
     5161 caagaccgtg catttgcaaa tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa
     5221 atcgttggaa acgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa aaccggcaat
     5281 gaatttgatt tagcagcaga cggagtattc atctatgttg gactggatcc attaactgca
     5341 ccttttagag aagcaggttt gacgaacgaa gctggctgga ttccaacaga caacgaaatg
     5401 aaaacgaata tgccaggcgt ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa
     5461 atcactacag ctgttggtga cggcggtatt gctggacaac aagtgtataa atacttggag
     5521 tctttagaag aaacaacagc ataagtttaa aaagagtaga agaacgggtt caattgttct
     5581 tttactcttt tttttcgcgt gaaatttact gaatctgatt tatgcgaaca cgtccaacac
     5641 cttttgataa aagaatcctt tcttgattta agcgtttttt ggtcctcata ctttcatatc
     5701 taataagtga atgctatact tattaagaac catatttcag aaagaggtgt tattttcatg
     5761 tcttgggaac aagtgtatga acaatgggta catgaagaaa atttagaaga aaacttaaaa
     5821 aaacaattaa atgatctggg tgaagatcct gaaaaacttg aagatgcttt ttatgcaccg
     5881 ttggaattcg gtactgcagg tatgcgggga atcttaggac ctgggatcaa tcgaatgaac
     5941 atctatacgg taagacaagc aacagaagga ctagcacgtt tcatggatac acaaggtgaa
     6001 gaaacaaaac gccgaggtgt cgctatcgct tatgactcac gccatcagtc accagaattt
     6061 gcaatggaag cagccaaaac actagcacat cacaatatcc cgtcttatgt attcgaaagt
     6121 ctgcgtccaa caccagagct ttcttttgca gttcgtcatt tgaagacgtt tgcgggaatc
     6181 atggtaaccg catctcacaa cccagcggct tacaatggat ataaagtcta cggagaagat
     6241 ggcggacaaa tgcctcctgc agacgcagat gcattgacga aatatgtccg tgaagtcagc
     6301 aatccgctaa aagtcgaagt attatctgac gaagaagcta aacatagcgg gttgatcacg
     6361 atcatcggag aagaagtgga cgctgcttat ctagaagaaa tcaaagcagt gacgattgat
     6421 cgtgaattgg ttgaaacaat ggggaaagat ttgaaacttg tgtacacgcc acttcatgga
     6481 acagggaaaa tgttgggcga acgtgcgttg aaacaagctg gattcgaaca attcgtacta
     6541 gttcccgaac aagctgtggc agatccagat ttcactacgg tcaaatcgcc aaatccagaa
     6601 gaacattcag cttttgaata tgctatacga cttggagaaa aagaaggagc tgacttgctg
     6661 attgcaacag atcctgatgc agaccgttta ggcgcagcgg ttcgtttgcc agatggttct
     6721 tatgaagtat tgactgggaa ccaaatcgga gcattgtttg ctcaatatat tttggaagca
     6781 catcaatcaa gaggcacttt accagaaaac gcagcggtat tgaaatcaat cgtttccagc
     6841 gaattgccta cagcaattgc ggaacactac ggtgctacca tgttcaatgt gttgacaggg
     6901 tttaaattca ttgctgaaaa aatccaacaa tttgaagaag accattcaca tacttttatg
     6961 tttggattcg aagaaagtta tggttattta gttaaacctt ttgttcgcga taaagacgcg
     7021 atccaagcat tgcttttatt agcagaagta gcagcttact ataaaaaaca aggcaaaacg
     7081 ttgtatgacg gcttgcaaac gatttttgaa acctatggct atttcgcaga aaaaacgatt
     7141 tctgtgacaa tgagcggaca agaaggttct gcgaaaatct ctgctttgat ggaaaaattc
     7201 cgtaaagaag caccgacaga atttgccgga gcaaaagtta ttcaaacaga agactttaaa
     7261 gaattgacga gaacagaccg tgaaggaaac gtagaaaaac taacaacacc gccatccgac
     7321 gtattgaagt atacattgga agaaaatggc tggatcgcta tccgcccttc aggaactgag
     7381 ccgaaaatca agttttatat tggtgtaaaa ggaagctcta cagaagacgc agccgaaaaa
     7441 gtagcctctt atgaagcgat tatcaaaaag atgacagaag aataaaaaac ttgagactga
     7501 tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg aatggattgc
     7561 cagacacgca aaaatcaaaa gtagagaaag tcagtcaatt attcaaaatg ttgagtgatc
     7621 ccactcgtct taaaattctg ctatatctga aagacgggga acaaaatgtg acagcaatca
     7681 cccaagcagt ggaaatggag cagtctgctg tatcgcatca actacgttta ttaagagaaa
     7741 atcatgtcgt caagtctcat cgcgagggaa aagcgatttt gtatagtctt gatgattctc
     7801 atgtactgga tattttgaac cagacactca aacatgtgga acatcaataa cgataggtga
     7861 aaatagataa acactggagt gtgacaacga agaaatttcg ttgtcacact ccagtgtttt
     7921 ttaagaaaaa gacaaaaatg attaatcgct tttttctgtt gtttcagttc cataataact
     7981 atgatcttct aggtctaaag cattcaaaat aagcgaagcc gtttcttcaa tagataaaga
     8041 tgccacattg atcactacac atcctagttt ttcataaaga tcattcgcaa aatcgagttc
     8101 agctctaatt ttttcgatat cggaataagc agtatcaggg tttaatccgt aagagcgcat
     8161 ccgttcttta cggatattat ttaaaatatc tgggttattc gttagtccca cgattttttt
     8221 aggatcgatt tcccacagct gtttgggaat atgtgcttgg ggaatcaaag gtaaattagc
     8281 aacttttaaa tttttatttg ctaaaaacaa gctcaaaggc gttttggaag ttcgggatac
     8341 gcctagcaaa acgacatctg cttctaaaaa accacgagga tctttcccat catcgtattt
     8401 tacagcaaat tccattgctt tgatccgttt gaaatagttc tcattcaata aatggagggc
     8461 accaggttgc cgaattggct tgataccagt taaacgttca atttctgata tcggtggcgt
     8521 catcacatcg aaactaaata attgatgttc ttcacaaaaa gaacgtgtta tctcgatcag
     8581 ttcttgattg attaatgtat gcaggacaag tgctttttct tctaatgcat cttgcaatgc
     8641 ctgccttaat gttttttcat ctgtcacgaa cgttctgcgg aataggttga actcaacggt
     8701 aggatactga gccatagtag cttgcgctag ttttgtcgca gtttctccag cagagtcgga
     8761 gatgacaaaa aaagtaacga tttctgtaga atcggaagac atggataaaa cctcacttgc
     8821 atttttttta tgtttattat accatactct agaatgcgta cttcgtaatc attttgattt
     8881 ggaggaataa gtcatgaaaa aacagacatt gatcgaacaa tcattagaaa cattgaaaaa
     8941 taacggtttt aaatatacta aaaaaagaga agcattgtta acttatttag tgaagcgtaa
     9001 ccgctacgta tctgctaaag aagtttttga ctatatgaat caggaattca aaggggtaag
     9061 ttatgatacg atttaccgga atctccatga ttttgcagag atcgatttac tggaagaaac
     9121 cgatttaaat ggtgaaatga agtttcgttt tcattgctgt cagggggaaa tcggccatca
     9181 ccatcatttt atctgtacag tgtgcggaac aacgaaagaa atccatatgt gtccaatgaa
     9241 ttatttcgaa gaacagctag gtggatgcac gatcgaaggg catagattcg agattttcgg
     9301 aagatgtgaa aattgtcaat aaggatttaa ataaaataac atttgataat ttaaaaaaaa
     9361 agcaagattt ccttgacggt caaaacagca ttagatataa tatttacagg acagtcttgt
     9421 tttttgagat atatttcaat aacaattacg gttacttttt aagctcggag ggagggaatt
     9481 aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct tcgtcgcttc
     9541 aaacgttccg tgtcaaaagc gggtacttta caagaatctc gtaaacgtga attctacgaa
     9601 aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa aaaattctag
     9661 ttctgtgatg aattggagtt ggtagtgtgt cactactaag tagattaaac gatgacatga
     9721 aagcagcgat gaaagcgaaa gataaggaat ctttacaagt catccgaatg atcaagtcat
     9781 ctatacaaaa cgagcagatt aaagaaggtc acgacttgac agaagaagaa gaactaacgg
     9841 tgttgtctcg cgaaatgaaa cagcgacgtg attctttgca tgagtttgaa gaagcaggac
     9901 gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat agtcgaaaaa tatatgccgg
     9961 aacaactttc cgacgaagaa attcgacaac ttgttcaaga agcaatcact caaactggtg
    10021 cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat tatgcctaaa gtcaaaggaa
    10081 aagcagatgg aaaccaagtg aatgcgattg tgaaagaact attacaatct taattttatc
    10141 atttaaactg cggacagtga ttgtccgcag ttttttgtgt tcgtaaaaaa agaactaaaa
    10201 aacagctaga agaaagaata agattctttc ttctagctgt ttttttcctg tttaatatct
    10261 cttatgttaa attgtgtttg atttggttat tcttctggta gttcatttgc ttcttgttta
    10321 taccctgata ggcgataggc gtaataaaag agtaagctgc cagcgtaacc aagtaacgtt
    10381 gggaccatcc agccaagtcc taatgcagca aaaggaacgt gatgatgata aaatcctagt
    10441 aagcttgcta cgacagtttg ctgactcact aatgcaggcg aatttgccaa catatccaat
    10501 acggctggaa cggcagaaaa gaacatcgtt acttgataga caaatggact cttttggaag
    10561 aattttgctg ttaaggaaag caagattaac gcaagtgaca atggatacaa aagcatcaat
    10621 actggtaaag accattggat gatattatcc aatccggcat tcgcaacgac aaaagagaca
    10681 aaagtcgtta accgcagcca tgtcatataa ctgactttag ggaacagttt atggaaatct
    10741 tgtgcaaaag aaacaacgag tcccatagcg gtagtgaaga cacctaatgt gaccattacc
    10801 cctaagaagc tagcaccgaa gttacctaaa taaaaattag tgatctgaga aagtgcagtt
    10861 ccgccatttt ctgataaagg catcttattc aaactcatcg caccgagaag gaccagacca
    10921 aaataaatca tgacttctaa cgcaacgctc aggctaccag cttttgctgt taattctgtg
    10981 acttttttat ctttaaaacc aagatcacga actgcatgga caaaagtgac acttaaagca
    11041 agtaaagcta ctgcatcaac cgtgttgtat ccttctaaaa cgccatttgt caaagcgttc
    11101 gtctgataag aatctgcagc agattggttt agattcccca tgggttggaa taaagaaacg
    11161 acaaaaatga tagctaataa tgcaagaaac aaggagttta aatactttcc gacatatttg
    11221 accaaacgtg ttggatgtac agtaaggaaa taagcaagta aaaagaaaag agcagagaat
    11281 aaaaacatac ctaatgaatc atatttttca ggcaaaaagg gtttagcagc catttcaaaa
    11341 gcagttgctg ctgtccgcgg tgtaccaaag aatggtccga tcgttaaatg aacaaggatc
    11401 aagaacaagg cagcatacca ttttccgaca ggtttagcta agtcatacag accatcactt
    11461 tttgtcacta ctacagctaa gatagctaat aatgggaaaa gagcgcctga gatggcaaat
    11521 cctacaccag cgctgatcca gtttgatcca gccatttgac ctaagtggac aggaaagatc
    11581 agattacctg acccgaagaa catcccgaag ataagtgaac tgaccatcaa taattgcttc
    11641 catgaataag tttttgtttc ttccataaat ttttcctcca ataattatca ttttgagtaa
    11701 caaaataaac gttgcagaaa ctagaaatgc cactaatatc agattccatg aaaaccacca
    11761 ccttaaaata taaaaaaaag cccttttcca cttgggaaaa gggctctaga ttctagaacg
    11821 gtaccacctt ttttcgctta aaaaagcttc ttcattcacg gacgcttttc gataaagtta
    11881 tccgctagcc tgataatgga ggcagccaca atgatctacg ttttcgacat tgactcggaa
    11941 atgatgttcg tctattcgat ttattgtcat ttctcactta catgactcac tgggaataaa
    12001 tcgctgaata gcctactgtt ttcctcgacg ctttgctgta tatttatttg ttgacgttat
    12061 aatacacctg ttttttttga aatgcaagaa tatttcaaat attttgtttt ctttttttga
    12121 aacaagaaaa atacccagag aatacttgct aagttagacg gaatacttgt cctctatctt
    12181 atgcatagct tacatgatca gcttttttta ttcaaacgag acctttagag aatggaagca
    12241 gctatttttt aacgtttctt ggtacttatg gttaagcaac tcttttagaa ccttttctta
    12301 tttgctatat agtttcttct gagtatggta aaataggaaa gaatgaatcg aaaaaaaggg
    12361 gatgtctatt tttgacggaa gagtctagtt ctttagagat tagattgacg gctgctgatg
    12421 atgtcagcat gcttttaggt gcacacgaca agcatataaa actgattgaa gaaacaacag
    12481 aaacaaccat ccatacacgt ggagaaatca ttcagatcat tggcgaacaa tcccagattt
    12541 ccttggctgc ctcagtcatt cggacattgc aggaattgat caaacgagga atccatgtaa
    12601 gtacaccaga tgtcgtgacg gcactgaaaa tggggcaaaa aggcacatta gattattttg
    12661 ttgaaatgta tgaggaagag atcgtcaaag accgtaatgg aaaaccaata cgggtaaaaa
    12721 acagcggcca aaagaaatat gtcgaatcgg tagccaaaca tgatatcgta tttggcgtag
    12781 gaccagccgg gacaggaaaa acttttcttg cagtagtatt agcaatcgct gccttgaaaa
    12841 aaggtgaagt gcaaaaaatc attttgacgc ggcctgctgt ggaagcaggg gaaaatctag
    12901 gctttcttcc cggggatcta aaagaaaaag tagatcctta tctgcgtcct gtttatgatg
    12961 ctttatacca aatttttgga ttagatcata cgaaccgatt gatggaacga ggcgtgatcg
    13021 aaattgctcc tttagcttac atgcgggggc ggacattgga tgatgcattc gttattttgg
    13081 atgaagcaca gaacacgaca gttgctcaga tgaaaatgtt cctgacacgt cttggttatc
    13141 aatcaaaaat gattgtcaat ggagatacga gtcagattga tctgcctcga gggacaacaa
    13201 gtggtcttgt tcatgcagaa cgaacattaa agcaaatcaa gaaaatcgat tttgttaatt
    13261 ttgaagcaag tgacgttgtt cggcaccctg tagttgccga aatcatcaaa gcttatgaaa
    13321 aggcagatct ccatcaagaa taagctttgc ttaggaggat aagcacatgt tgaataaaac
    13381 actgcagaaa ctacaagaaa aacttggaaa ggcagcattg cctttcctgc tgttcttgtt
    13441 ttttatcatt atctgcggcg ttacatttag tagtgtcaaa caaaaggcca atgatttcag
    13501 agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa acgatcgaga acacggaaga
    13561 aaccgaacaa aaaagaaaac tggcagcaga agcagtcact ccagaataca cttaccaaaa
    13621 agatcttgca gatgaccaga acaatcggat caagcaacta tttgaattga tcgataaaac
    13681 gaatgatgcc atcaacaaat cttatgacga aaagattgat aaggcaaagg aagatgaaaa
    13741 tatcccaaag cctactacag aagagcggat cgccgcgttg aagaaaaatt ttgaaaatgt
    13801 gaatgcagaa aatgttgcgt tttatcaaaa actaccgaac aatttttatt cgacgatatt
    13861 tgaaatgact gaaactgaga tccatactgt gagggatgag agtctgaagc tcattgacga
    13921 acagatgagc aaacaagttc gtgaaagtga attggaagct tttaaacaag aagcagaaga
    13981 gcaaatccaa tatttgaatg tcacaccagc acaacaacaa atgattcgct acttagtgga
    14041 tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag acagaagaat tgaaacaatc
    14101 cgcacgagaa gctgtacaac cagtgatgat cttccaagga gaaatcattg ttcgtgaagg
    14161 taatcaaatc gatgcttcag ctatgaaaaa attggaatta ctaggactga ctaatcaaac
    14221 aacttcgatt tttccgctaa ttgccatggt tttagctgtt ttgcttcaaa tcggcgtact
    14281 gatttataat tcacttcaat tccaaaatgc aggagaaaga acaaaatacg tgcttttcta
    14341 tgtgacagct atgtcagtca gcatagtgct gatgaaattt ttccaattat tccaaacgga
    14401 acaagctact tatatcccgc tgttttatcc cgctgctttt gcaccacttg ttttaagctt
    14461 tttcttgaac cgaagagcag ggatcatggc tggtattttc caagcagtat ctgcactttt
    14521 cattttttat gattcgattg gcacgaactt tctaacgatt atcttgatgt cttatgtttt
    14581 ctctgggctc atggcaacga ttgtcagacg caaacggatc tcagaacaag gattatctgc
    14641 tgccatgtgg gtcattattt ttcctgtgat gatggatgtc attttgatcg tctatcaagg
    14701 aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc tgcgggttca ctggtgcact
    14761 cttctcattc ttgctgacga tcggtctaca cccatatatt gaattgatgg tcaatgatga
    14821 cagtgtaatt gtattaaatg aactcagcaa tccaaatcat cctttattga aacagttgct
    14881 ggaagaagcg ccaggtactt atcatcatag tatgatggta gctaatctaa gtgctaacgc
    14941 tgtagcagaa atcggcggac gttctttgct aactcgagta gcttgctatt atcatgatat
    15001 cggcaagatc aaacatgcta gctttttcgt tgagaacttg ccttcaggag cggaaaaccc
    15061 gcacaatttc ttattgccag aagacagtaa acatattatc tttggacatg tgacagatgg
    15121 agccaaaata ttggaagatt ataatatgcc gcaaatggtg atcgatattt gcaaacagca
    15181 tcatggaacc acgctaatgc gttatttcta catcaaagcg aaagaacgca atccggaagt
    15241 aacagaagag caattccgtt atcctggacc aaaaccgcaa agtcgtgaag cagggatcgt
    15301 caatattgct gacagttgtg aggctgcagt acgggcaatg gatcatccga cgagcgaaaa
    15361 aatcgaaagt tttgtccata atcttatcga agagcgtatt tccgatggtc aattagacga
    15421 tagtggactg accttgaaag aaatccgaaa agtggaaaaa tcattgatca gcggtttaag
    15481 tagcaccttc cattctcgga tcaaatatcc taaaatgaaa acagaagttg agaagataaa
    15541 ggaagaacaa gaagaataga aagaagcgat gtataatgga tattaccttt atggatgaaa
    15601 caaaccaagt ctcagaagag aaaatgaaag aaatcgatga cttgctgcaa tttgcagcat
    15661 cttatttaaa gctgccagag gaaacagaaa tgtctgttac tttcatggac aatgcggcaa
    15721 tccaagtaat caatcgtgat taccgtggaa aagatgcacc gacagacgtc atcagttttg
    15781 ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt tgaagatgga gagaatccat
    15841 tgcctcgtgt actaggagac atcatgattt ctattgaacg tgctgctgaa caagcagaag
    15901 cttacggcca ttcatatgat cgggaacttg gctttttagc ggtacatgga ttcttgcata
    15961 tcaacgggta tgaccatatg acaccagaag atgagaaaga aatgtttggt ttacagaaag
    16021 agatcttgga tgcctatgga ctcaaaagat aaaaacgtag agaaaaacaa acatttcctg
    16081 atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt ttgaggaaga acgcaatatg
    16141 aggaaacatg ttgcgttcgg tatccttgct ttatttatgg gcgtgatttt ccagcttgat
    16201 cggatagagt ggttatggct gcttttagct gtttttctcg tttggatagt agagatcatc
    16261 aatactgttt ttgaaaatgt agtggacatg tttacggatt ttcattttca tccgattggg
    16321 aaaaaaatca aagatatggc agctggagca gttttagtga ccgcttgttt tgctgtcatc
    16381 attgggttga ttttatttgt ccctaaaata tatcatttgt tgtttaatta aaaaggagaa
    16441 cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt cggccgcccg aatgtaggaa
    16501 aatccacatt attgaaccga atcgtgggtc aaaaaattgc cattatgagt gacaaggcac
    16561 agacaactag aaataaaatc caaggagtat atactacacc agaagctcag ctgattttta
    16621 tcgatacacc aggaatccat aaaccaaagc atcgtctagg agattttatg gtagaagcag
    16681 cttacagtgc gttgaaagaa gtggatgcag tcttatttat gatcagtgct gatcaaaaac
    16741 gcggccgggg agacgatttt attattgaac gtctaaaaaa cgtgcattct ccagtttatc
    16801 tgattatcaa taaaatcgat aagatccatc cagacgagtt gctgggaatc atcgaagatt
    16861 attcgacgca gatgcctttt gcacaagtga ttccgatctc tgctacagaa ggaaacaatg
    16921 tcgaacgctt aatggatgta ttagtcagtg aaatgccaga gggcccacaa tattttccag
    16981 atgatcaagt gactgatcat cctgaatact ttatcgtttc tgaattgatc cgtgagaaag
    17041 tcttgttttt aacacgagac gaagtgcctc attctgtcgc agtcgttgtt gattcgatga
    17101 agcgaaacga gaatgataaa atccatatcc aggccacgat catcgtcgaa agagatagcc
    17161 aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa gcaagtagga acgaaagcaa
    17221 gaaaggatat cgaaaatctt ttaggagaca aagtatttct agaactttgg gtcaaagtcc
    17281 aaaaagactg gcgagacaaa aaagtctatc tgcaagactt tggctaccga aaagacgaat
    17341 actgatattc tgaacttctt ataggttaag tatagaaata aactggataa aaatccgcac
    17401 gttgagaaaa acggcggaag atcgaaacat aaataatctg gatcggcaga atctgctttt
    17461 ccagatttat tttttaggag ggagaatatg agccagcttg cagaatcgaa aggcttgatt
    17521 ttatctagcc gtaattacaa agaaaaagat aaattagtca aaatctttac ggaatcttct
    17581 ggcaaaatga tgttttatat caaaggaatc catcggcaaa atcagccatt agctccggct
    17641 ttgctcccct ttaccgaagc agtctatatc ggtaattttc gaagtgaagg tttgtcgttt
    17701 ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc aagaagatat ctttttgagc
    17761 ggttatggta cctatttgct aaatctagta gatgcagcaa tcgaagatca tcaatatgac
    17821 ccgcatttat tccaatttac gcaacaagct ctgcagcgga tggatcaagg agatgatgca
    17881 gaaatcatca cgaatatctt tgaagttcaa atactgcaac gcttcggaat cgcgccaatt
    17941 tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat ttgactattc ttccaaatct
    18001 ggcggtgtga tatgcgagaa acactggcca atggacgaac accgttatca tgcagatcct
    18061 cgcgctgttt atttcgttcg aatgttttcg gctatttctt atgataagat cagtggaatc
    18121 cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca ttgaccagtt gtatgatgaa
    18181 tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc aaatgaagtc ttgggcacac
    18241 gtattaaaga cgccagagaa aaatatgcct gaaaaagaga tacctgaaaa agaagaataa
    18301 atgaaagata caacaaaaaa atcaaaacta tttgacaaat acagggactt ccgtctatta
    18361 ttaaccgtag attaaataga tcgttcactg agaggatcta ttatataaac aagaaaactg
    18421 caaaatgaat acgtacattg accaagatca gtaaaaaaca cgtggtttag cgagttcggg
    18481 agagtggaag ccggacccaa acgatttttg aagaggcact tgcgagtacg ataaatgaag
    18541 ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa ttattcgtcc ctatgtctgt
    18601 aatcagacat agggactttt tgtcgttttc tttcctgtat tcttattgac agcaacgctt
    18661 aaaaattgaa aggggcaaaa cagatgagta aaaaattgac cgtccaagat atgattttaa
    18721 cattacaaaa attttggtca tcaaacggct gcatgctgat gcaagcttat gacacagaaa
    18781 aaggagcagg tacgatgagc ccatatacat tcttacgggc aatcggaccg gaaccatgga
    18841 atgctgctta tgtagagcca tcccgccgac cagctgacgg gcgttatgga gaaaacccaa
    18901 accgtttgta tcagcatcat caatttcaag tcgtaatgaa accttcacct gaaaatatcc
    18961 aagagcttta tttagaaagc ttgcgtcttt taggaattga tccattagaa cacgatattc
    19021 gttttgttga agacaactgg gagaacccat cgatggggtg tgccggtgtg ggttgggaag
    19081 tatggcttga tgggatggag atcacacaat tcacgtactt ccagcaagtc ggaggactac
    19141 cttgtaaacc agttacttct gagatcactt atggactaga acgtcttgct tcatatattc
    19201 aagaagttga aagtgtctat gatctggaat gggctgatgg tgtgaaatat ggcgagatct
    19261 tcaaacaacc agaatatgaa cattccaaat attcttttga agtaagtgac caagacttgc
    19321 tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg ctgtatcgat gaaaatcttg
    19381 ttcatccagc gtatgattat attttgaaat gcagccatac attcaattta ttagatgctc
    19441 gcggtgcagt atccgtaaca gaacgagcag gttatctttc ccgtatccgc aacatggctc
    19501 gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt aggattccct ttattgaata
    19561 aaaacacagc aaatccagaa aacatgaagg aggctgaata agatggcaaa agacttatta
    19621 cttgaaatcg ggctggaaga aatgcctgct catgttgtca caccaagcat gaaacaatta
    19681 gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga cttatgatac gatcgaaacg
    19741 ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca ttccagaacg tcaagcagac
    19801 agtgaagaag aagtcaaagg accagccaaa aaaattgctt tggacgcaga tggaaactgg
    19861 acgaaagcag ctgaaggatt tgtccgcgga caaggtctga cgacagatgc tatcactttt
    19921 agagaattaa aaggcgtgga atatgtctat gtaacgaaat acactcacgg caaagaagcg
    19981 aaagaagtat tgacaggttt agcagatgtc attaaaagcc tcactttccc agtaacgatg
    20041 cattgggcag aatacgattt tgaatatatc cgccctattc actggatcgt ggcattgtta
    20101 gatgacgaag taatcccgtt tgagatctta gatgtcaaaa caggcagagc ctctcgagga
    20161 catcgtttct taggcgaaga tgtgacattt gcgcatgcaa aagaatatga agaaaaattg
    20221 aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag aaatgatcgt tacacaagca
    20281 aatgaattag ccgaaaaaaa tcactgggtt attgatctag acgaagattt actagaagaa
    20341 gtcaacaatt tagtcgaata tccaactgtc ttttctggcg acttcgaaga aaaatatctt
    20401 tctgttcctg aagaagtact agtcacttct atgaaagaac atcaacgtta tttcgaggta
    20461 cggaacgatc aaggtatgct gcttcctcat tttatttctg ctcgtaatgg tgatcgggta
    20521 aaattggaaa atgtggttcg aggcaatcaa aaagtattga cggctcgttt agaagatgcg
    20581 gaattctttt ataatgaaga caagaaagct tcaatcgacc aatacgttga aaaactaaaa
    20641 actgttactt tccatgaaaa aatcggtagt atctatgaga aaatgcagcg ggtacaagtc
    20701 attgcccgta tcattggcga acaagtagga ctatctgagg aagaattgat ggatctgcaa
    20761 cgagctgctg aaatctataa atttgatcta gtaaccaaca tggtagggga atttcctgaa
    20821 ctacaaggga tcatgggcga aaaatatgct ctgctgaaag gcgagaaacc tgctgtagca
    20881 acagcgattc gtgagcatta catgcctata tccagtgaag gagagctacc tcagtctaca
    20941 gtaggttctg ttttagcgat tgcagataaa ttagatagca ttttttcttt ctttgcagtt
    21001 gggatgattc caagcgggtc aaacgatcct tatgccttac gtcgccaagc atacggtgtc
    21061 gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt ctgttctgca agaaacgatt
    21121 agtgatgtta tcagtaaaga tacggatcgt ttcggcattg gtttatcaga agggcaacaa
    21181 caggtgatcg actttatcaa aggccgtctg cgccaattat tgacaacgaa gaatattcgt
    21241 catgatgtca tcgaagctgt tttgaatgca gaacaaaaag atctgacaaa agtatttgca
    21301 gcagcacaat tgttcaagca gcatcttgca gatgaagact tcaaaccatc gatggaagct
    21361 ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg aaaaacaatc agaagtagac
    21421 cctgaactgt tcgaaaatga agcggagaaa gaactgcaca aagcagtaga ggcaatgaaa
    21481 aaaagctttg ctgaccaaac gatcaacgaa aattatgatc agcttgtaag ccttcgccca
    21541 ctgatcgaaa attattttga tcagacgatg gtcatggcag aagaaccagc agtacgtaag
    21601 aaccgattaa cgcaattaag tcaaattgct gccatggctt tgtcgctagc aagtttagat
    21661 caaatcatca caaaataatt tctaatatgt aaataaacca gagaataggc acaaaatagg
    21721 ctatttctct ggtttatttg ttataaatat aaataactat acccgtggtg ctagttaatt
    21781 ttttcctagg tattatacaa gtaatttgag tcggtcaatt tcaactgaat tatctaaact
    21841 aactgatcta cttttagata attcagttga aatttattgg taaactgtcg cttttctttt
    21901 taaatggcaa aaatttttaa taatatatga caatttagaa atcaactata agactgatta
    21961 attcaaaatg atcttagaat cattgtatga acactaaata ctatattgtt ttctattaat
    22021 cgttatataa acgattatga attcaaagct actttttatg acgtcaat
//
LOCUS       DACGLH010000037        21556 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.039, whole genome shotgun sequence.
ACCESSION   DACGLH010000037 DACGLH010000000
VERSION     DACGLH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.039"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            214..498
                     /locus_tag="IXT21_001625"
     CDS             214..498
                     /locus_tag="IXT21_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ4869032.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IXT21_001626"
     CDS             complement(779..1198)
                     /locus_tag="IXT21_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ4869033.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IXT21_001627"
     CDS             complement(1200..1784)
                     /locus_tag="IXT21_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ4869034.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IXT21_001628"
     CDS             complement(1831..3306)
                     /locus_tag="IXT21_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ4869035.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IXT21_001629"
     CDS             3487..3798
                     /locus_tag="IXT21_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869036.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IXT21_001630"
     CDS             complement(3913..6249)
                     /locus_tag="IXT21_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4869037.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IXT21_001631"
     CDS             6391..7059
                     /locus_tag="IXT21_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4869038.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IXT21_001632"
     CDS             7062..8105
                     /locus_tag="IXT21_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4869039.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IXT21_001633"
     CDS             complement(8342..9223)
                     /locus_tag="IXT21_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4869040.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IXT21_001634"
     CDS             9382..10110
                     /locus_tag="IXT21_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ4869041.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IXT21_001635"
     CDS             10266..10583
                     /locus_tag="IXT21_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4869042.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IXT21_001636"
     CDS             10576..11184
                     /locus_tag="IXT21_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ4869043.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IXT21_001637"
     CDS             11200..12030
                     /locus_tag="IXT21_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ4869044.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12680
                     /locus_tag="IXT21_001638"
     CDS             12168..12680
                     /locus_tag="IXT21_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ4869045.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="IXT21_001639"
     CDS             12706..13578
                     /locus_tag="IXT21_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ4869046.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="IXT21_001640"
     CDS             13754..14116
                     /locus_tag="IXT21_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4869047.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="IXT21_001641"
     CDS             14125..14877
                     /locus_tag="IXT21_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869048.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="IXT21_001642"
     CDS             14891..16693
                     /locus_tag="IXT21_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4869049.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18011
                     /locus_tag="IXT21_001643"
                     /pseudo
     CDS             16970..18011
                     /locus_tag="IXT21_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(18063..20207)
                     /locus_tag="IXT21_001644"
     CDS             complement(18063..20207)
                     /locus_tag="IXT21_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4869050.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..>21556)
                     /locus_tag="IXT21_001645"
     CDS             complement(20564..>21556)
                     /locus_tag="IXT21_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4869051.1"
                     /translation="GLVVLATTIGWLSETSGTYHILSAASNAVFYFLPIFLAFSAAKT
                     FKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTSTVMPAILGIFFY
                     SFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGLAAAITWMMDIN
                     GPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAANFGMAGAALGV
                     FLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIGAAAGGAVMGFA
                     QVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFDEDLMSYE"
BASE COUNT         6662 a         3837 c         4439 g         6618 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aaactatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgtta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa ggttgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgactagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg taatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aagacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgcat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg catgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcgt
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatattctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatgct ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattggttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacc
//
LOCUS       DACGLH010000038        21501 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.040, whole genome shotgun sequence.
ACCESSION   DACGLH010000038 DACGLH010000000
VERSION     DACGLH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.040"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            2..400
                     /locus_tag="IXT21_001646"
     CDS             2..400
                     /locus_tag="IXT21_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ4869052.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IXT21_001647"
     CDS             384..1079
                     /locus_tag="IXT21_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ4869053.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1663..2550)
                     /locus_tag="IXT21_001648"
     CDS             complement(1663..2550)
                     /locus_tag="IXT21_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ4869054.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2735..3574
                     /locus_tag="IXT21_001649"
     CDS             2735..3574
                     /locus_tag="IXT21_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ4869055.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3770..4744
                     /gene="sgrA"
                     /locus_tag="IXT21_001650"
     CDS             3770..4744
                     /gene="sgrA"
                     /locus_tag="IXT21_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ4869056.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5042..5398
                     /locus_tag="IXT21_001651"
     CDS             5042..5398
                     /locus_tag="IXT21_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869057.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5401..6558
                     /locus_tag="IXT21_001652"
     CDS             5401..6558
                     /locus_tag="IXT21_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869058.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKVEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6584..7024)
                     /locus_tag="IXT21_001653"
     CDS             complement(6584..7024)
                     /locus_tag="IXT21_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ4869059.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7184..7690
                     /locus_tag="IXT21_001654"
     CDS             7184..7690
                     /locus_tag="IXT21_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4869060.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7858..9771
                     /locus_tag="IXT21_001655"
     CDS             7858..9771
                     /locus_tag="IXT21_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ4869061.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9776..10777
                     /locus_tag="IXT21_001656"
     CDS             9776..10777
                     /locus_tag="IXT21_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4869062.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10979..11185)
                     /locus_tag="IXT21_001657"
     CDS             complement(10979..11185)
                     /locus_tag="IXT21_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ4869063.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11831..12679)
                     /locus_tag="IXT21_001658"
     CDS             complement(11831..12679)
                     /locus_tag="IXT21_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4869064.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12843..13745
                     /gene="gnd"
                     /locus_tag="IXT21_001659"
     CDS             12843..13745
                     /gene="gnd"
                     /locus_tag="IXT21_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ4869065.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13747..14748
                     /locus_tag="IXT21_001660"
     CDS             13747..14748
                     /locus_tag="IXT21_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ4869066.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14745..15029
                     /locus_tag="IXT21_001661"
     CDS             14745..15029
                     /locus_tag="IXT21_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4869067.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15062..15859
                     /locus_tag="IXT21_001662"
     CDS             15062..15859
                     /locus_tag="IXT21_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4869068.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15873..16706
                     /locus_tag="IXT21_001663"
     CDS             15873..16706
                     /locus_tag="IXT21_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4869069.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16724..17581
                     /locus_tag="IXT21_001664"
     CDS             16724..17581
                     /locus_tag="IXT21_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4869070.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17603..18112
                     /locus_tag="IXT21_001665"
     CDS             17603..18112
                     /locus_tag="IXT21_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4869071.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18149..18550
                     /locus_tag="IXT21_001666"
     CDS             18149..18550
                     /locus_tag="IXT21_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869072.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18662..19015
                     /locus_tag="IXT21_001667"
     CDS             18662..19015
                     /locus_tag="IXT21_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869073.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19049..19492
                     /locus_tag="IXT21_001668"
     CDS             19049..19492
                     /locus_tag="IXT21_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4869074.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19509..20594
                     /gene="uxuA"
                     /locus_tag="IXT21_001669"
     CDS             19509..20594
                     /gene="uxuA"
                     /locus_tag="IXT21_001669"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ4869075.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20587..21300
                     /locus_tag="IXT21_001670"
     CDS             20587..21300
                     /locus_tag="IXT21_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4869076.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7398 a         3301 c         4490 g         6312 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga atatattttt
     1681 tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga ttgttttatc
     1741 catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct cagatttgta
     1801 tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat aatagctttg
     1861 ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt tttttagttt
     1921 aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa aattcatttt
     1981 tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct tggagaaatt
     2041 ttccccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg aaaatagaat
     2101 gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat ctttatctgt
     2161 taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa agagtttctt
     2221 atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg gaccgagttt
     2281 tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt ttccgtatac
     2341 atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat ttgatttaac
     2401 ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg ttgcaatcgt
     2461 ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa ggcacataat
     2521 tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag tctaacagaa
     2581 gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt agttcgtaaa
     2641 aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa gaaagaaaag
     2701 ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc ggttgattgg
     2761 atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata atgcacttgc
     2821 ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg tcggacattt
     2881 agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa gaatagaaga
     2941 ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc tcgttgttgg
     3001 aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt atcggttaga
     3061 ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta ttgcagtggg
     3121 ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta gatttggtcc
     3181 gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat tgaatcctaa
     3241 catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca gcgcacttat
     3301 ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga attggacaca
     3361 aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt tctccactct
     3421 gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag catctatatc
     3481 aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat ataaaacaaa
     3541 gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa ggctgatatc
     3601 ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat tcaagcgaat
     3661 acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca ttttcatttt
     3721 atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa tgaagaaaac
     3781 agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga ttggaatcag
     3841 tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa ctgatcggat
     3901 tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca aaagcgtgaa
     3961 tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa gttcagaaaa
     4021 agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg agtatacaat
     4081 aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag ttgatttgat
     4141 tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca gttggacagt
     4201 tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag aaaacacgag
     4261 cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca cgagcagcga
     4321 aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca gcgaaagcag
     4381 tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga gtagcacagg
     4441 aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt tggtacctat
     4501 cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg tgctaaaaca
     4561 gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa cagataatag
     4621 aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta catggatggg
     4681 tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc atcgagtgaa
     4741 ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt gagtgaatat
     4801 ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc tatttttgtt
     4861 tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata agagcaagaa
     4921 taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac gaatctattt
     4981 gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag gagtatgtac
     5041 tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt tgaactattg
     5101 ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc taaactacac
     5161 cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa ggtatccaga
     5221 atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt cttctcagtt
     5281 tttactgagt aaagtttatt ctatactttt agagggaacg attggttata ttttactgga
     5341 ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa ttatataagt
     5401 ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa ctatcattta
     5461 tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat ccgatacttt
     5521 tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt atcaagtata
     5581 aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac gcgtagtata
     5641 gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa acaaggccac
     5701 tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat aacaaaagaa
     5761 gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc gatgaatcac
     5821 atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa cacctatttg
     5881 gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg gattgaatcg
     5941 tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat
     6001 tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg gacaaaaaag
     6061 aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat atttccagtg
     6121 atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc agagcttaat
     6181 acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt cagggatatt
     6241 tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg taaaacacag
     6301 gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa atatagtgca
     6361 tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa aaaggcatat
     6421 gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa tgattgggcg
     6481 cgtctgaaag ttgaaatcat aaaaatcagg aatgagaaaa atagatccat attggaaaaa
     6541 ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac attgcaaaaa
     6601 gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg agctcttgct
     6661 ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca gtcaaaagtt
     6721 ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta cgaatctcaa
     6781 tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta taactaatag
     6841 aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt
     6901 catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata tcatggacga
     6961 taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac aatttaatca
     7021 tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa atagtcgaaa
     7081 agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa ctagctagat
     7141 aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa acacagccga
     7201 cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc cgatacacga
     7261 attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa cgattgggaa
     7321 accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa aaccatggga
     7381 gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa
     7441 ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc attcgatcga
     7501 attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg ctcatggatt
     7561 aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc cttttatgaa
     7621 agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc tgacattttt
     7681 ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt tatccattct
     7741 gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata aaccatttat
     7801 tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata aaaaggagtg
     7861 aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt tgctgtaagt
     7921 acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc ggaatctcgg
     7981 gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa
     8041 acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga tcaagaaacg
     8101 accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac
     8161 gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga tgatatcggt
     8221 aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca gatgacagaa
     8281 atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt agtcactgag
     8341 cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat tgctacttat
     8401 ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca
     8461 gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa acaacttcat
     8521 caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata ttatgtcgat
     8581 caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg gaataacggg
     8641 atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt atttgttgat
     8701 tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata tgagtcagat
     8761 gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa gattttactt
     8821 tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga tattcctgtc
     8881 aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca actacgtttt
     8941 acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca gttagacatc
     9001 attggggatc aagagcatga agatttattt atgggccttt tgaaacaata ctattttgat
     9061 caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc agattcattg
     9121 cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa
     9181 ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc agggcatgga
     9241 aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt tttgaaagta
     9301 gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag tctactcaaa
     9361 gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac agagcaaaag
     9421 acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat ttttaatgag
     9481 acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc aaaaaagcaa
     9541 gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga tcaatcaagt
     9601 gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa
     9661 agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc aggtggattt
     9721 ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta aatgaatgaa
     9781 atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt tagtcactgt
     9841 atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa aacaatttga
     9901 cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa aaatcgaaca
     9961 aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata atatggatat
    10021 ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc atggaggcgc
    10081 atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat gttcagacac
    10141 gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac atcctacacc
    10201 agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag atcaagcaga
    10261 ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg gagaatattt
    10321 gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga ctttggagaa
    10381 caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg cacttactca
    10441 agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt actttgataa
    10501 taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac atgcggatcg
    10561 ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga aacaaatgga
    10621 agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag attatgcgga
    10681 gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac aagcgcaaga
    10741 aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt
    10801 gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag cttttcatgg
    10861 tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca ccttttattc
    10921 gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat tttataagtc
    10981 agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa
    11041 gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt ccgttgttat
    11101 tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta gcgaaaaaaa
    11161 gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt gtatgataaa
    11221 tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag
    11281 cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta
    11341 caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag
    11401 atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt
    11461 ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc
    11521 aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa
    11581 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
    11641 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
    11701 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
    11761 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
    11821 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
    11881 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
    11941 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
    12001 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
    12061 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
    12121 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
    12181 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
    12241 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
    12301 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
    12361 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
    12421 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
    12481 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
    12541 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
    12601 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
    12661 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
    12721 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
    12781 taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta
    12841 tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa
    12901 aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa
    12961 gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga
    13021 aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac
    13081 tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct tattataaag
    13141 attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga
    13201 cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag
    13261 aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt
    13321 atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg
    13381 gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg
    13441 acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg
    13501 aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt
    13561 ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc
    13621 cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag
    13681 caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg
    13741 agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt
    13801 ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa
    13861 gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt
    13921 gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc
    13981 cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc
    14041 gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat
    14101 ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg
    14161 gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt
    14221 gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt
    14281 ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta
    14341 gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa
    14401 gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta
    14461 tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc
    14521 ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat
    14581 ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa
    14641 tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt
    14701 cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg
    14761 tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt
    14821 caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa
    14881 cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat
    14941 ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa
    15001 tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct
    15061 agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag
    15121 taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg
    15181 gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat
    15241 tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga
    15301 atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca
    15361 agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga
    15421 tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt
    15481 tgctggttta gttggaggat taggagataa atacgcaaga aatgtggcag cacatgctat
    15541 gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt
    15601 agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt
    15661 atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc
    15721 gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca
    15781 tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg
    15841 ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt
    15901 tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt
    15961 tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag
    16021 ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc
    16081 caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat
    16141 tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg
    16201 gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca
    16261 aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg
    16321 ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat
    16381 tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag
    16441 taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag
    16501 attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata
    16561 ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc
    16621 aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg
    16681 aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga
    16741 taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag
    16801 atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact
    16861 atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc
    16921 atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac
    16981 tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat
    17041 cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg
    17101 aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc
    17161 ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta
    17221 tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac
    17281 aggcctaaag caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt
    17341 gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc
    17401 gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt
    17461 acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac
    17521 acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata
    17581 aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt
    17641 gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt
    17701 tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag
    17761 ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga
    17821 atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga
    17881 cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa
    17941 tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa
    18001 attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc
    18061 tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc
    18121 ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga
    18181 atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct
    18241 ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga
    18301 tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt
    18361 tatctgtact tattacgata cacccgttca tgcattagga agttatttca tgtttgattg
    18421 gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag
    18481 tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct
    18541 atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa
    18601 agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac
    18661 gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga
    18721 aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat
    18781 tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt
    18841 ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta
    18901 tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg
    18961 agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa
    19021 aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa
    19081 gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga
    19141 aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa
    19201 attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag
    19261 tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac
    19321 ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct
    19381 tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac
    19441 tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg
    19501 ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa
    19561 aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc
    19621 ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag
    19681 gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag
    19741 atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg
    19801 gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt
    19861 ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga
    19921 tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg
    19981 gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga
    20041 ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg
    20101 aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt
    20161 ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc
    20221 catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag
    20281 ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt
    20341 ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata
    20401 tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc
    20461 atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa
    20521 tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag
    20581 agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact
    20641 ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt
    20701 aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc
    20761 attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa
    20821 gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta
    20881 tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa
    20941 gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg
    21001 gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg
    21061 aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca
    21121 aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat
    21181 aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat
    21241 gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa
    21301 taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat
    21361 gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa
    21421 caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga
    21481 atcgtccata tgagggtttc c
//
LOCUS       DACGLH010000039        21071 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.041, whole genome shotgun sequence.
ACCESSION   DACGLH010000039 DACGLH010000000
VERSION     DACGLH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.041"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..570
                     /locus_tag="IXT21_001671"
     CDS             <1..570
                     /locus_tag="IXT21_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4869077.1"
                     /translation="APGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLT
                     PGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVE
                     TQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTG
                     ETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(994..1164)
                     /locus_tag="IXT21_001672"
     CDS             complement(994..1164)
                     /locus_tag="IXT21_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4869078.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1358..1537)
                     /locus_tag="IXT21_001673"
     CDS             complement(1358..1537)
                     /locus_tag="IXT21_001673"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869079.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1547..1771)
                     /locus_tag="IXT21_001674"
     CDS             complement(1547..1771)
                     /locus_tag="IXT21_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869080.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1924..3036
                     /locus_tag="IXT21_001675"
     CDS             1924..3036
                     /locus_tag="IXT21_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4869081.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3114..3407)
                     /locus_tag="IXT21_001676"
     CDS             complement(3114..3407)
                     /locus_tag="IXT21_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ4869082.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3575..3889
                     /locus_tag="IXT21_001677"
     CDS             3575..3889
                     /locus_tag="IXT21_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ4869083.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3910..5889
                     /locus_tag="IXT21_001678"
     CDS             3910..5889
                     /locus_tag="IXT21_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4869084.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5902..6228
                     /locus_tag="IXT21_001679"
     CDS             5902..6228
                     /locus_tag="IXT21_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ4869085.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6244..6762
                     /locus_tag="IXT21_001680"
     CDS             6244..6762
                     /locus_tag="IXT21_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869086.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6762..8114
                     /gene="celB"
                     /locus_tag="IXT21_001681"
     CDS             6762..8114
                     /gene="celB"
                     /locus_tag="IXT21_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ4869087.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8198..9703)
                     /locus_tag="IXT21_001682"
     CDS             complement(8198..9703)
                     /locus_tag="IXT21_001682"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ4869088.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9795..10385)
                     /locus_tag="IXT21_001683"
     CDS             complement(9795..10385)
                     /locus_tag="IXT21_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4869089.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10564..12210
                     /locus_tag="IXT21_001684"
     CDS             10564..12210
                     /locus_tag="IXT21_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4869090.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12259..12978
                     /locus_tag="IXT21_001685"
     CDS             12259..12978
                     /locus_tag="IXT21_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ4869091.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13457..14404
                     /locus_tag="IXT21_001686"
     CDS             13457..14404
                     /locus_tag="IXT21_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4869092.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14416..15336
                     /locus_tag="IXT21_001687"
     CDS             14416..15336
                     /locus_tag="IXT21_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4869093.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15377..16834
                     /locus_tag="IXT21_001688"
     CDS             15377..16834
                     /locus_tag="IXT21_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4869094.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17179..17793
                     /locus_tag="IXT21_001689"
     CDS             17179..17793
                     /locus_tag="IXT21_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4869095.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17786..19510
                     /locus_tag="IXT21_001690"
     CDS             17786..19510
                     /locus_tag="IXT21_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4869096.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19507..20958
                     /locus_tag="IXT21_001691"
     CDS             19507..20958
                     /locus_tag="IXT21_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4869097.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6796 a         3563 c         4244 g         6468 t
ORIGIN      
        1 gcaccaggag tgtatcagct ggtagaaacc caagcgccaa cggggtatga attggatgcg
       61 acaccaatcg agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa
      121 aatcgactta caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggagaagtc
      181 ctgcaaggag ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta
      241 accacaggag cagatggaaa actggcgata gatggcttag caccaggagc atatcagctg
      301 gtagaaactc aagcgccaac ggggtatgaa ttggatgcga caccagtaac tttcaagatc
      361 gaaaaagaac aagaagtggc gatcttttta acaaaagaga atagaaaagt tgctgatagc
      421 tcagatataa gaggaaacaa tccaacaagc agtggaaata agcatcttcc aaaaactgga
      481 gaaaccatca ttagccagtt tatattgtca atattgggtg tgctacttgt tttcatcagt
      541 ataaaattta gaaagaaacg aaatatatga gctaaaataa aaaattgaat aagcatagtt
      601 tttaaaatca actagtaccc atcaattgaa tggaaactac cgattttaga aggggtatac
      661 aaacaaatat tagtatcgga gtagagaagg ttgaacccaa ccagtaaata agtaaagaca
      721 attttgtgct gcgttgagaa gtgaaaaata aacctttgta gtaatactag ttaataaaaa
      781 atatacaaaa acactgcgaa aatcattctt gagtttcgca gtgttttttg cctaatgact
      841 caagtttatt gggtaatact ttgattttct ttctacaaat aatcagcgat cagttgtaat
      901 tttttctcta gttttaatgg ctctttgtca ataaggagaa aaacaagtcg ggtcttttgt
      961 ctcggaaagc ttcaaccaac tcgtgaataa aggttacgcc tccttgaaag gcgtggaaaa
     1021 accaagtaat cgatcaatca ccaatttcgc atttggtaga acatgtttgg tgagctgaaa
     1081 gtaggtggcg ttcatgtctg taactaagaa ttctatttct ttaggattag tgcagctaag
     1141 gaaatagttt gttaatcgag gaattttttg tgtaggtaaa ttttctcgat taatttttct
     1201 gtctcgccat ccgcggaaat aaaactcagt ttatcctcaa cggaaacgtg agaacgaaat
     1261 tcacaaccct caacactcaa gacagaattc ttttggaaga ctttggtaaa tagcttctca
     1321 actctttcaa tgtgcgaata acggttgtca aagataccta acaacttttc gaaataaaaa
     1381 ataacgacac tttttcagtc agtaaagaag tgattttatg tctgacatga tttgaaatcg
     1441 agtgccttgg acggataaaa taactttgag cgttccaatg gcttcgacag tttttacagg
     1501 tatagcgctg cttttttagg cgtataacca ttggcatatg attatattat tcaaagtgga
     1561 caatggtttc cttcttcttg tttttcacca ccattaattt cctatttcca tctaccacag
     1621 cagaaccaca atttctacag acacatggag cagggaagag aactgcagca acgatcaatg
     1681 tttttttctt ctgtaaggtt tcgtaagaga cctctgtaat catcaaattt ttgtctattg
     1741 atctcagaat ttttttgata gaaccattca taaagcatat cgttctctca gttgtttatt
     1801 ttgtggtgat ttaatcatac aagaaaacga tatgtttttt tataaaaaca tgaataggaa
     1861 aaatagttga caagaaaatg acaacgccat atgatgttcc atagtattta caacctgtca
     1921 tagatgactg taaaaaataa aagtaaaaag aaaggacgac aacaggtgga tttttatcag
     1981 tctctacaac tagatccgtt tattttaaaa caaaaaatac gagaagcagc ttctaaaaaa
     2041 gaaaagtgca tgtatatttg ttcattgttt ttacgatcat tattcatcgt attgtttgcc
     2101 atttgtttta tcattataat tacaacatta tttgaatcga agcataaacc atacgctgtg
     2161 gttttattct gtatgttgat gagtattcgt tttgttgact ttggttataa gatttcccat
     2221 tcgatcatag gtttagcaat cgtcatgttc tcactattag tagcaccgta tgtccagctg
     2281 atcaaatggt cagttatggg catgttgatt cattttattt tactaagcag catattaatg
     2341 gcaaccgcta gtgatcccaa aatggggaat gcttctttgt acggcttttc atatctcttt
     2401 attgtttatt cgttacctaa agattcgttg aacaagaatt tctttacaca aacaagtagc
     2461 ttattatttt tgtttttttg ttggttttca gttctccttt acagaaaaca tcgagaaaag
     2521 aacaaagaca aaagcttgtt taaagagatt tttttgaaag gcatgtattc gcaggaaaaa
     2581 atttggatgc tcatctatgc atttggcatt agtcttttga tcgttgcagg agagtatgtc
     2641 ccttttcagc gattgatgtg ggcagggttt gcattttctt ctattgtatc tagctatgga
     2701 ttgatgtcga ttggtttcaa agaacgagca gtagaccgaa tcattggctc attgattgga
     2761 tgtgttttat ttattggtat cagccaattt attcctttta actgggtcgg tatattaggt
     2821 ggtctagctt taggtgtttg ttctacctat agatataaaa cagtttttaa ttcttttggt
     2881 gctttggcaa tcactgccag tttattcggg gttccaggag cagtaacgat ccgcattttt
     2941 gagaatatcc ttggggtatg tttaggaatc atgtatattg gggtgactga aatacttatc
     3001 cgaaaaataa gagaaaaaca cggtttgaat cattagatga gatcgaataa aaaataagga
     3061 aaaagtgaag atacacatgg atgataaatt gctatcattc catgtgtatc tttttacggt
     3121 aaatcagtga aacgttcgac tttcattttt aatttatact ttttacggag ttcgtttatt
     3181 tgagccatca tttcggattt gtgatgcttt tcgattgctt cttcatcttt ccaacggtcg
     3241 atcagtaaaa gagattctgg atcttctagt ggaaagtaat agtcatattt ctcgtttcct
     3301 tcttctgcac gaatcgcttt gacaatgttt ctttcgacca tttcttctgc aaattttctg
     3361 gcttgtccat ttgtacttgt atacaaaata ttcactgtga tactcactaa atcccctcct
     3421 gaaaaaattc tatcactagt atatcacggt gccagaaatt attattcaag atatgataca
     3481 cattttttcc gcaatgctac ggaaatatat attttgaata gaaaacgctt acaagatatg
     3541 cttggtttat aaaaataaaa ggagtgattg atttatgaaa aaagcattga ttatttgtgc
     3601 ggcaggaatg tcatcctcga tgatggcctc aaagacaacg gatttcttta agcaaaaaag
     3661 agaagaggta ctcgtagatg cagtttcagc tacagaaggc gataatatga ttaagaatag
     3721 tgattttgat ttatttctga tcagcccgca aacgacgatg tttttggata aattcaaaaa
     3781 attaggagaa tctgttggaa agcctgttgt tagtatccct ttccaagcgt atgtcccaat
     3841 ccctacaggc atccagaagt tagcagaggt gatcgaagag aacatatgat ggaataggag
     3901 tggatgataa tgaaacagaa ggaaaaattg ctgttgagat atttgattga tcacaagaag
     3961 gagtatgtca ccagtcaacg tttagcttct gaattgttat tatctgatcg gacaatccgt
     4021 aattacttgc agcggatcaa agagttagta gaaaaaaacg gaggtaagat cattgctaaa
     4081 ccaggttatg gctaccaact gcatatttta caaagactga catttgactt gtttttgagt
     4141 cggcaagaaa tagtacctgg atatgctaga gaaccgcaag gattttatga gtccgaagat
     4201 cgacaaaagt atatattgaa ccaattgctt ttggaagatg ccattctttt tatggatgat
     4261 ttggcagaag agctttatat cagtcgttcc agtttgacaa aggacatgca agaaatcaag
     4321 gagcgattga ttccatattc actaaaaatc gtttctaagc atggtcaagg gacatggatc
     4381 gaaggggaag aaagaaatcg acggcatttt atcatggata ctttttttgg aaaccattat
     4441 ggaaattcat tgaaagaata tttagggcaa agccgttttt tcccagacat cagttttgaa
     4501 gagctagtca ttatcattct cgatgaaaca agagaagcta agttaaaagt ttcggatttt
     4561 atcatacaga atctgacttt acacttggct ttggggatca aacggctccg agaagggttt
     4621 gaaatcaagg aattaggcat cgaacaagaa gtttctaagc gagtagagta tcaagtggcg
     4681 cagcggatcg ttcaacgtat tgaggtaatc gcaggtgtat cgtttcccga agaagaaatt
     4741 gcttatttga cattacatct catggctaag tcgaatcaca aagaaaatca aaatgatcag
     4801 gaactatcaa aggaattagc gaaagttctg aaaagattgt ctcaggaatt tcatttttca
     4861 ttagtagagg atctccagct tttcaatgga ctactcgatc atttgtcacc gatgatgatt
     4921 cgattgagcc gaggtattcg cattgagaac ccattgactg aggagataaa gaaagaaaat
     4981 cccaaagtct ttgacgcagt aaaaaggcat ttcagcaaca tgccggcttt gaaaaactat
     5041 accatcaatg aagatgaatg ggcctatttg gctttgcatc tcatggcggc attggaaaaa
     5101 gaaagagctg cgcataaact acatgcattg attatctgtg caacgggtta cggcagtgcc
     5161 caactattaa agaatcgcgt agtcagtgaa ttcggtaaaa atattacagt ggtaagtgta
     5221 aaaggctatt atgagatcaa tgaaacaact ttaaaaggaa tcgacctgat tatttcttct
     5281 attgatcttt cgacaatgtt tttcaaaatt ccagttttac atgtcagtat ttttttgaat
     5341 gaagatgatg ttcgtaaaat acgaaaggta gttggagaat ctatacccta tcattcctca
     5401 gatagaccga taccagcatt ttctctacag caaaaaaagc agatttacac cgaacagttg
     5461 tctgaaaaat ttttcaagac ttatacgtac gaaccgaaaa aggaagaaat tcttcatgat
     5521 ttgctgcaaa agctgagcat aaatgaagaa gaaggatata taagagaaat gcgaaagcag
     5581 atccagcaac gtgaaaatat gggacaaatc atattcagtg atacagtggc agtcccacat
     5641 cccgctgttc cagttggaat cgaaacaaag gtagccgttg cattgattcc ttctgggatg
     5701 cagtgggatc aatatcaaga aattcatttt gtttttttag tttcgccttc ttatgtcgaa
     5761 aatgaaggaa ttacggtcgt gactaaagca atcgttaggt tagttgacca gctaaaagta
     5821 cagcaggaaa tcttagctga acctattttt gagaatttca gcagtaaatt tatcaaactg
     5881 atcgaataag gaggaaaaca gatggctgaa aaaataaaca gtgaaacact acaagtgact
     5941 tcttttgata tcattttcca tagcggaaat gcacggacac tgatacatga agcatttcgc
     6001 tcgatgcgta aagagatgtt tgaagaagta gaagagaagt tgcgacaagc aaatgaagag
     6061 attcttcatg cgcatcagtc acaaactcaa cttctgaaag aatatgccag cgggacaaaa
     6121 atagaaatgg aaattatcat ggtccatgca caagatcatc tgatgacgac gatgacatta
     6181 ttggaagttg cacaggaaat gaattatttg tatcaaaaaa ataaatagaa aggaaggatc
     6241 attgtgcagg atacagtaaa aaggaaaatc gaattagaaa aaaagcaact tagtttgaaa
     6301 aacatgtatt tcaatcgtta tctaatcgta cgttacctga ctgccttttt ctttttcatg
     6361 aacatgcaat ggatgattct gcttctatca gctaaaagtt taggaagtag tttaccgatg
     6421 attctgattt tagctatttt gcctgcagta ggagaacaag taaagcttta tcgaaaacac
     6481 caaacgaacg ttccatggac aaagtggtat tttttgttcc agagtgtctt taatttatta
     6541 gtgattccag tgttgtttac ctcaggattt acattcttgt atccttttat ggcaaataac
     6601 aatggaggac agttatttgt tttcattctt gttgtaagcg gtatttttgc cagtgcattt
     6661 atacagtatc gtttaaagaa aatttcactc aaccaagacc aacagtatat ccgtattaaa
     6721 caatatgaaa aagcgttata tttaggaaag gagaataact aatgtttgat tttttgcaga
     6781 aatacctgat gggacctatg gggaaaattg cgcaacttaa aatcgttcgg gctgtcatgg
     6841 cagcggggat ggcttctatt cctttcacga ttgtaggctc gatgtttttg gttttaaata
     6901 ttttgccatt accgttccca tttttagaag gattttttaa tgcaactttt ttcaaagtta
     6961 gtgacttgta catgatcgtc aatacaatga cgatggggat tttatctatt tactttgcca
     7021 ttgtgtttgc ttatgaatta acgtcaattg aacgagatga acaaaaatta aatgttaatc
     7081 cgctcacggg ggcattacta tctgtttttg cattctttat gtgtatccct gaattgatca
     7141 tgactgatgg taaaatcagc ttgattgctt ctatcaatga gacagaaact gtagtaaatg
     7201 gtattaggat gggctctttt gtcgaacgtt taggaacttc aggaatcttt acagctatta
     7261 ttatgtcgat tattgcggtc gaattgtatt gtatgtgtgt caagagaaat tgggtcataa
     7321 aaatgccaga tgttgttccg ccaggagttt ctcgttcttt cactgctctg atcccaacat
     7381 ttgtcattgc gtttgtggtg atgatcatca atggattatt agttgcacta ggaacggata
     7441 tcttcaaaat gattgctatc ccatttgggt ttgttacgca gttgacgaat acatggcttg
     7501 gtgtcatggt gatttatttc ttgatccatg cattgtggat cgtagggatc catggagcga
     7561 atatcatcac ttctttcttg accccgatcg tattagctaa tatggcagct aacgcacaag
     7621 gcgcaaatta tccgttggca ggagaattca acaattctta tgtaaccgtt ggtggatcag
     7681 gtgcgacttt aggattgatc atctttattg cattcctcgc gaaatctgat cagctgaaag
     7741 tgttaggaaa agcctcactt gtaccaggat tattcaatat caatgaacca atcattttcg
     7801 ggatgccaat cgtctacaat ccctatttag cggttccgtt tttcttagct ccgatggctt
     7861 ctgctacact cgcttatttt gccattcggc tggaaatcgt caaaccgatg cttgcacaga
     7921 tgccgtggcc ttcaccagtc gggatcggag catttgttgg tagtggtggt gattggaaag
     7981 ctgctgtgtt agcagtcatt tgtgcaatca tcgcattcat tatctggttc ccatttatca
     8041 aattctatga taataaatta ttaaatgaag aaagagaaaa ggcagcagaa ttagctagtg
     8101 aaaagttaaa atgatttcta agaaaaaagg aactgtgaca aaaaatcaca gtcccttttt
     8161 ttcttagaaa tgggaaacca ctagtatata aataccatta ttcttcaatc aaccgcttca
     8221 cgattgcata atccccttca taaggttcat ctacaccatt gattttttga tagggatcca
     8281 cgccttttcc cgcaataacg actgaatctt gcggagcgct ttctgctaaa gcgcgtcgaa
     8341 tcgcttcagg acgatccatt tcataatgaa cgatcaaatc tggattagta attgcttcat
     8401 tgatttcttc cgcgattttt ttcggatctt caaatgctgg gtcatctgcc gtcaaaaagg
     8461 cgatgtcagc tgtttctgat agcacttttc caaaatcatg tcgacgggag attgctttgt
     8521 ttcctgggct gcctaataca acaattacac ggccatctgg atgttcattt ttggcaaatt
     8581 tcagcaatgt tttcaagctt agataattat gtgcatagtc cacatagaca tgcgctccat
     8641 tggattggat caattgatcc attcggcctg gtacacgagc ttcttttaat ccgtcttgtg
     8701 catcttgact ggaggcacct gttagtgctg cggcaatgat cgcactggct gcattttctt
     8761 ggttgaatcc tccagccagc aaaatatcat aagcagtatc gcagatagct aagcgatctt
     8821 catgagaaga taaagtaaaa ccgtgagtac ctttatctga acggatcact tgataattgc
     8881 tttcttccga acgaccataa gtgatcgttg gaatctggaa tagatcggct gtttccctaa
     8941 gtaaatcaaa atagtcactt tcatgattta aaacaactgt ttttgaattc aagatcaatt
     9001 gtcgcttgca atagaaatag tcatcaaatg tcggatgttc gatcggactg atatggtcag
     9061 gcgagatatt caagaagatt ccgacatcta aagtcaagcc gtatacgcgt tgtgttttgt
     9121 aagcttgtga agaaacttcc atcactagat gagtcatacc attttcaact gcttctgaca
     9181 tcatacgata taaatctaat gactcaggcg ttgtcaaatg ggatttgaaa tatgttttgc
     9241 catctaacgt tgtattcata gtagaaagca atgctgtttt tttatttgtt gtgtgatcca
     9301 agattgcttt tgtaaagtac gcagcagttg ttttaccttt tgttccagtg aatccgatca
     9361 ctttcaattt attttgcgga tagtcgtaaa aagccatagc aagaagcgcc attgctttac
     9421 ggatatccgt tacgatgatt cctactgctg aattttcgta tggttgttct gatacataat
     9481 attgaatacc ctgttcgatt gctgaatcca aatattcttc tttgaaattc attcctttac
     9541 agaaaaacaa agtagaagca tcagcttttc ttgaatcata acttaactga agaaattcag
     9601 ctttttctgg tacatcaaga tgccagcctc ttgttgaaac aaattctttt aatagatttt
     9661 cttttaatag taaatgacgg atgtcttcta aagagaagct cattgattga tccctcgctt
     9721 tattcaattt cttggtttat tatagcacac tctttttaat atatcgtttg tttccgctat
     9781 gacaaaatcc agctttattc aaattttctt gacacagggg acaagcggat gagtattttc
     9841 ataaacaatg aactgactgt aatagatagg agaacgaata gtgtctccaa caatccaaaa
     9901 acataataat tcaaaaagac gataagacta ttggtaagta aaagtccttg cggcattgga
     9961 agccgaaaaa atttgtggaa aataatcggc ggtatcgtta taccgccatt tgaggccccg
    10021 actgtgtaca atgtgtaaac cccaagactg aacaaaagac ttcctgaaat cagtgaaaag
    10081 aaatgtgagg agatcatttc cacttttgga acaatctcta aaacaacagg aaaaagcaga
    10141 cttcccgcta cagtcttaaa aaatactgat tttcctaata ggacagcggc tagaagcagc
    10201 atcataagat tgatgctcca aacagtaact gagcgttgga tagaaaaggc cgtttcaact
    10261 aaatgtccga tggcgcccac tccggcggaa gctatatcgt gagggagcaa gaaccaattc
    10321 aatccaactg tgataaagaa aagacctaaa aatataacgg gtagtttttt tatttcttgt
    10381 gtcattgata tctcctataa atgttagcca taaaaggctg tatatttctt tactttacca
    10441 aaaaaacgaa accgaattca aaaaaacaat tgttcttata aaagttatat aaaatcgatt
    10501 gccagtttct gttttaccaa ttaaaatgat agtaatgttt tgtagaaagt tggaatctga
    10561 ccgatggata atcaaagaaa accagcatta gatcaattaa caccgcaaga aaaaatggca
    10621 cgaggctctg cttggatgac cgccagtaat atcatttcaa gattgctagg cgctgtctat
    10681 atcattccct ggtatgcttg gatgggcgaa aatgctaagg ctgccaatgg attattcaat
    10741 atgggataca atatatatgc attgtttcta ctgatctcta cagcaggaat acctgctgcg
    10801 attgcaaaac aaactgcacg ctataattca ttgaatgaat atggcacttc acgacgatta
    10861 tttatccgtg cattacagat gatgggcggg ttaggcctgt tgtttgcttt gttcatgtac
    10921 atcgcctctc cttggctagc tcatgcttct ggtggcggag aagaactgat tccaacgatg
    10981 cgttctttga gtatcgctgt attggttttt ccttgtatga gtgtgatacg agggtatttt
    11041 caaggcaatc aagaaatgat gccttatgcg ctgtcgcaga ttgtagaaca ggtggcacgt
    11101 attttttata tgctattggc tacattcatc atcatgaaag tcatggatgg tgactatgtg
    11161 acggcggtca cgcaatcaac tttcgcggca tttatcggta tgctagcaag tattcttgtc
    11221 cttttgtatt ttttgaagaa acatcaagcg tatacatcgg cgtttattca ctacagcgaa
    11281 aacaaaataa atattgcaac gaaggaattg ttgttagata ccgttaaaga agcgattccg
    11341 tttattattg tgggttcagg ggtaacgatc ttcaaattag tcgatcagtt cacttttatg
    11401 aagatcatgt ctgcatccac cgagtattcc aatgcacagt tactggatct tttttcgatt
    11461 tttagtgcca atccagataa gttgacgatg gtagttattg cattggcaac atcgatcgcg
    11521 tctactgggt taccgttgat tacagaagct gttacgttga aagatcgagt aggtctggca
    11581 aaattgacaa gcggaaattt acagttgttt tcatttttta tgtttccggc gacatttggt
    11641 gttatgttac ttgcctatcc gctaaataca ttattttata caccggattc tctaggaagt
    11701 aatgtactga tccaagcaag ttttgtagga ctcttcctag gactatatat gctagtttcc
    11761 aacatgctgc aaggaatgtt tgaaaacaag gcggcaatta attatttggt tattgggttt
    11821 ctagtgaaac tccttcttca atacccagcc attcgtttat ttgaagtata cggcccttta
    11881 ttggcgacaa tgatcggatt tgctgtgtct tgtaccttga tcttaaagag aatccatgaa
    11941 gtagcccgct ttaaaccaag ttttgtctgg cgcaggacat tgctgatctt tattcttacc
    12001 ttgattatgc ttttggcttc gtggatcaca aagatgattt ttggtgcttt tttaaacgaa
    12061 gatagcaaat tccagtctct attgttgatt cttgtagtag caggtatagg ggctttagtg
    12121 tatggttatt tagctttgaa aatccgcttg gcagaaagac tactaggacc aggaatagcg
    12181 cgtttacgac ataaattaaa aataaaataa gtgaatcaga agagctagat tgatgctctt
    12241 ttgaaatggg ggaaacgcat gcgtttagat aagtttttag cagatatggg acttggcagc
    12301 cgcaaagaag tcaaaggatt aatcaaaaaa ggaacgatcg aagtcaatgg acaagtggtg
    12361 aaaagcgata aagcgcaagt aaatgaattc gaagatgcgg ttacttattt aggagaaatc
    12421 attacgtatc aaaaagattt ttactacatt ttgcataaac ctgcaggagt catttctgct
    12481 actcaggata atcatgatca aaccgtaatc gatttattgg aagaccagga ttatcgggaa
    12541 gatctttttc cagtcggccg attagataaa gacacagagg gcctcttgat tttaacgaat
    12601 gacggggtgt ttgcccatca gttactatca cctaaaaaac atgtggagaa agaatatcta
    12661 gctgaggtta gcggtataat gacagaagaa gatatccgtt tatttgcaga aggactgatt
    12721 attgatggcg atgagcagac attgccggca aaactccaaa ttgatcaagt agatcaagag
    12781 acagaaactt caattatccg tctgattttg catgaaggaa agtttcacca agttaagcga
    12841 atggtcaaag cagtaggaaa agaagtctta tatttaaaaa gaatccgtat gggaaaattt
    12901 tggctgccta aagatttgaa taaagggcaa tatcgaccca tgaccaaaga agagctagaa
    12961 caagtgagag aaaaataatt gaatggatag ctaaaagaat aagcagagta gttgtagcgc
    13021 catctgaaaa gcagacttgt ccgtctgact ttcggatggc ttttttgtga aatttcggct
    13081 tatttccgta gaagctactt ctggaacacc gtttgttcgg aataagggac tgtgacaaga
    13141 cttttgttac agtccccatt gttccttgtg actcagagtt agatagtaag gacacgatct
    13201 ccctaatact caggccaaaa cgctgaattc acaacctctg ataaacggtg ttcaatcagc
    13261 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttc ccgttttttt
    13321 ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg tttcattttc
    13381 cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt gcgaatgaat
    13441 cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta tgaatctatg
    13501 gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga tttttttctt
    13561 ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt cagcagatgg
    13621 gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca aatttttatt
    13681 ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg tagcatttat
    13741 ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt tgagaaataa
    13801 acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat cttgggtcat
    13861 tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca atcagtggat
    13921 cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt ggccgtttat
    13981 cttggtgttc attggtacat ggaaagggat tggttataac agcattattt actttgcatc
    14041 tgttatggga atcgatccga cttattatga agctgctatg gttgatggag ctagcaaatg
    14101 gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga ttttaacgat
    14161 tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca ttcctaggaa
    14221 ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca atggcctgac
    14281 atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag ttgtcggctt
    14341 tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt ctgcattatt
    14401 ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg ctctttcaat
    14461 aagacggcta attttttctc taatataatg attgcactct ttgctatttc ctgtattttg
    14521 ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc gactcacggg
    14581 tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt cgaccagatg
    14641 caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt aggtacactg
    14701 atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa ctttccattc
    14761 cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg gatggtggcc
    14821 aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc attgatcttg
    14881 ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa acgttcggtc
    14941 tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg aatatttgtt
    15001 cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt tgccgcattg
    15061 ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa gctagttcca
    15121 ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca aaattcagga
    15181 gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc cagaatggcg
    15241 atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca gaagcatttt
    15301 gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat aaatcataaa
    15361 attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg tttcagcgtt
    15421 gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg cagaagctag
    15481 caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt tcgatcaatt
    15541 gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca atatcaatta
    15601 cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg gggaaaatta
    15661 tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct ttgctgattt
    15721 gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg aagcatatat
    15781 taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa atgtctttgc
    15841 tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt caattgatgg
    15901 aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa aagaaccaaa
    15961 cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg attatccttt
    16021 agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa tcatcaacca
    16081 atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt acaaacaagg
    16141 actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag gaaatacttg
    16201 gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt tgacaaatgc
    16261 agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta cagctcaagc
    16321 acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat cagtagaact
    16381 tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg ggatcgaggg
    16441 agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag atggttatca
    16501 gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat atacacaaga
    16561 gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg cagaaacttc
    16621 accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa gtaatatttc
    16681 taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc cagatgaaac
    16741 attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat tgaaagaaat
    16801 gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa aggggttgtg
    16861 acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc cagtttctcg
    16921 gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt tttcttacta
    16981 ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc agctaaccct
    17041 cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg tttctctaat
    17101 aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag tataaactta
    17161 atgtttggag gagaagatat gttagggaag gttttggaag agttatttat ccggatctgg
    17221 gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg gatcatcttt
    17281 ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg attcgagtat
    17341 aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt aagaggcaat
    17401 ctgctattta gtttattttt atctggtact gctttattga gttacaattt atatttatct
    17461 gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt tgccttgctc
    17521 tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac gatctctgtt
    17581 cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc ttttctaaaa
    17641 ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg attgatcctg
    17701 tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca ttggcgtgaa
    17761 gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt caaaaggaac
    17821 cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa cgatgactgc
    17881 taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag aaaacatgac
    17941 aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct tgagtaggat
    18001 catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga cctacttaac
    18061 aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta ccgatgaatt
    18121 agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac taagtgcgat
    18181 atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa acaaaggtgg
    18241 ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt tgcctatcac
    18301 acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag aactagatat
    18361 gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt ctggaacgac
    18421 aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg aaagacaaga
    18481 aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg atctggaaaa
    18541 aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag ctgtactaga
    18601 taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca ttggtatcat
    18661 tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat attcacagca
    18721 agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag agacgcggat
    18781 cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca acgaaatgtt
    18841 ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac agcaagacgc
    18901 acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata cattagaata
    18961 tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg tgtatgcgtt
    19021 ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa aagaagaact
    19081 ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc gagtagccta
    19141 tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg tgatccagcc
    19201 attagttgaa aattatttta aacacggtat tgattttact cgattcgata atgcgctaag
    19261 tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata atggaaaagg
    19321 gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta aagtggaact
    19381 tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct tcggcagcag
    19441 ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta ttacattcaa
    19501 ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat tttgaatggg
    19561 ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac agcagagaat
    19621 gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt aacggatgtg
    19681 acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga acgtcataat
    19741 tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg aggtatgcaa
    19801 ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt tgaaagcctc
    19861 aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca agaaatctat
    19921 caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag tgaagaagaa
    19981 ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact ggcaagaact
    20041 catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta ctatcaacga
    20101 aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt tgatggcttc
    20161 tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat cggggaagag
    20221 acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa tctacagttg
    20281 catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc agaaacaaaa
    20341 gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca attggaagtc
    20401 gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat gatgccggaa
    20461 gatatacgat attccagttt tttactgttt atggagattc aacgggaatt gattgatctg
    20521 gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac ggtgcaagaa
    20581 cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa gtacagtgac
    20641 aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact tacactcaaa
    20701 gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt caaaaaagaa
    20761 acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc gaaacaactg
    20821 cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta caacaacacc
    20881 acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga gtaccgagaa
    20941 aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata aaaaattgtc
    21001 tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt tcatgacctc
    21061 tcgataaacg g
//
LOCUS       DACGLH010000040        21007 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.042, whole genome shotgun sequence.
ACCESSION   DACGLH010000040 DACGLH010000000
VERSION     DACGLH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.042"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            168..362
                     /locus_tag="IXT21_001692"
     CDS             168..362
                     /locus_tag="IXT21_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869098.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IXT21_001693"
     CDS             631..930
                     /locus_tag="IXT21_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869099.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IXT21_001694"
     CDS             complement(1027..1461)
                     /locus_tag="IXT21_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869100.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IXT21_001695"
     CDS             complement(1693..2403)
                     /locus_tag="IXT21_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ4869101.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IXT21_001696"
     CDS             complement(2393..3253)
                     /locus_tag="IXT21_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ4869102.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IXT21_001697"
     CDS             complement(3258..3896)
                     /locus_tag="IXT21_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ4869103.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IXT21_001698"
     CDS             complement(3911..4210)
                     /locus_tag="IXT21_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4869104.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IXT21_001699"
     CDS             complement(4253..5737)
                     /locus_tag="IXT21_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ4869105.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IXT21_001700"
     CDS             complement(5757..6218)
                     /locus_tag="IXT21_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4869106.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IXT21_001701"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IXT21_001701"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ4869107.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IXT21_001702"
     CDS             7521..8291
                     /locus_tag="IXT21_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4869108.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IXT21_001703"
     CDS             complement(8564..9391)
                     /locus_tag="IXT21_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4869109.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNPMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IXT21_001704"
     CDS             9959..11194
                     /locus_tag="IXT21_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ4869110.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IXT21_001705"
     CDS             complement(12193..12453)
                     /locus_tag="IXT21_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ4869111.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IXT21_001706"
     CDS             complement(12480..13781)
                     /locus_tag="IXT21_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4869112.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IXT21_001707"
     CDS             complement(14073..14531)
                     /locus_tag="IXT21_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ4869113.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IXT21_001708"
     CDS             complement(14544..15869)
                     /locus_tag="IXT21_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ4869114.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IXT21_001709"
     CDS             complement(15886..17298)
                     /locus_tag="IXT21_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4869115.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IXT21_001710"
     CDS             complement(17315..18079)
                     /locus_tag="IXT21_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ4869116.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IXT21_001711"
     CDS             complement(18183..19058)
                     /locus_tag="IXT21_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4869117.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IXT21_001712"
     tRNA            complement(19313..19397)
                     /locus_tag="IXT21_001712"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IXT21_001713"
     tRNA            complement(19415..19486)
                     /locus_tag="IXT21_001713"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IXT21_001714"
     tRNA            complement(19496..19568)
                     /locus_tag="IXT21_001714"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IXT21_001715"
     tRNA            complement(19630..19714)
                     /locus_tag="IXT21_001715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IXT21_001716"
     tRNA            complement(19735..19805)
                     /locus_tag="IXT21_001716"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="IXT21_001717"
     tRNA            complement(19851..19922)
                     /locus_tag="IXT21_001717"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="IXT21_001718"
     tRNA            complement(19934..20006)
                     /locus_tag="IXT21_001718"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="IXT21_001719"
     tRNA            complement(20009..20082)
                     /locus_tag="IXT21_001719"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="IXT21_001720"
     tRNA            complement(20089..20169)
                     /locus_tag="IXT21_001720"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="IXT21_001721"
     tRNA            complement(20171..20246)
                     /locus_tag="IXT21_001721"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="IXT21_001722"
     tRNA            complement(20282..20354)
                     /locus_tag="IXT21_001722"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="IXT21_001723"
     tRNA            complement(20364..20436)
                     /locus_tag="IXT21_001723"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="IXT21_001724"
     tRNA            complement(20452..20523)
                     /locus_tag="IXT21_001724"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="IXT21_001725"
     tRNA            complement(20538..20627)
                     /locus_tag="IXT21_001725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
     gene            complement(20667..20740)
                     /locus_tag="IXT21_001726"
     tRNA            complement(20667..20740)
                     /locus_tag="IXT21_001726"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20704..20706),aa:Asn,seq:gtt)
     gene            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="IXT21_001727"
     rRNA            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="IXT21_001727"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6617 a         4409 c         3339 g         6642 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcggatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa cctgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag cgtggcgacg
    20761 tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct taacttctgt
    20821 gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg gtgttatctt
    20881 ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt ttttttccga
    20941 gtacttttct tttaacctat tggttaagtc ctcgatcgat tagtatcagt ccgctccata
    21001 catcact
//
LOCUS       DACGLH010000041        20871 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.043, whole genome shotgun sequence.
ACCESSION   DACGLH010000041 DACGLH010000000
VERSION     DACGLH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.043"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(192..662)
                     /locus_tag="IXT21_001728"
     CDS             complement(192..662)
                     /locus_tag="IXT21_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ4869118.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="IXT21_001729"
     CDS             992..2632
                     /locus_tag="IXT21_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ4869119.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="IXT21_001730"
     CDS             2786..4108
                     /locus_tag="IXT21_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ4869120.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="IXT21_001731"
     CDS             complement(4176..4610)
                     /locus_tag="IXT21_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869121.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5511)
                     /locus_tag="IXT21_001732"
     CDS             complement(4675..5511)
                     /locus_tag="IXT21_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ4869122.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6253..6879)
                     /locus_tag="IXT21_001733"
     CDS             complement(6253..6879)
                     /locus_tag="IXT21_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869123.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7174..7509)
                     /locus_tag="IXT21_001734"
     CDS             complement(7174..7509)
                     /locus_tag="IXT21_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ4869124.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7496..7780)
                     /locus_tag="IXT21_001735"
     CDS             complement(7496..7780)
                     /locus_tag="IXT21_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ4869125.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7836..9359)
                     /locus_tag="IXT21_001736"
     CDS             complement(7836..9359)
                     /locus_tag="IXT21_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ4869126.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9352..10527)
                     /locus_tag="IXT21_001737"
     CDS             complement(9352..10527)
                     /locus_tag="IXT21_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ4869127.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10531..10716)
                     /locus_tag="IXT21_001738"
     CDS             complement(10531..10716)
                     /locus_tag="IXT21_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869128.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10682..12376)
                     /locus_tag="IXT21_001739"
     CDS             complement(10682..12376)
                     /locus_tag="IXT21_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ4869129.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12373..12846)
                     /locus_tag="IXT21_001740"
     CDS             complement(12373..12846)
                     /locus_tag="IXT21_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ4869130.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12915..13070)
                     /locus_tag="IXT21_001741"
     CDS             complement(12915..13070)
                     /locus_tag="IXT21_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ4869131.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13204..13584)
                     /locus_tag="IXT21_001742"
     CDS             complement(13204..13584)
                     /locus_tag="IXT21_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ4869132.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13588..13803)
                     /locus_tag="IXT21_001743"
     CDS             complement(13588..13803)
                     /locus_tag="IXT21_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869133.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13806..14213)
                     /locus_tag="IXT21_001744"
     CDS             complement(13806..14213)
                     /locus_tag="IXT21_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ4869134.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14479..15954)
                     /locus_tag="IXT21_001745"
     CDS             complement(14479..15954)
                     /locus_tag="IXT21_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ4869135.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15944..16792)
                     /locus_tag="IXT21_001746"
     CDS             complement(15944..16792)
                     /locus_tag="IXT21_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ4869136.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16829..17191)
                     /locus_tag="IXT21_001747"
     CDS             complement(16829..17191)
                     /locus_tag="IXT21_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869137.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17491..18115)
                     /locus_tag="IXT21_001748"
                     /pseudo
     CDS             complement(17491..18115)
                     /locus_tag="IXT21_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18112..18423)
                     /locus_tag="IXT21_001749"
     CDS             complement(18112..18423)
                     /locus_tag="IXT21_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869138.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18467..18760)
                     /locus_tag="IXT21_001750"
     CDS             complement(18467..18760)
                     /locus_tag="IXT21_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869139.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18954..19601
                     /locus_tag="IXT21_001751"
     CDS             18954..19601
                     /locus_tag="IXT21_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4869140.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19662..20807
                     /locus_tag="IXT21_001752"
     CDS             19662..20807
                     /locus_tag="IXT21_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4869141.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         6084 a         4250 c         3475 g         7062 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     5101 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5161 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5221 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5281 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5341 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5401 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5461 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5521 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5581 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5641 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5701 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5761 agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca
     5821 gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt
     5881 ttaaggaaga gactgtgaag tcaacgtttt gacctgatta ttagagaaac aaatggcaaa
     5941 atagttcttc atatttcgcc gtttttcgag cgaatgccga ggggcagctg attgaacatc
     6001 gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac aatccctttt tttatatctt
     6061 attaattcat tcttttgttc tattcttctt cttccgccat gaatgagaaa cccatatcaa
     6121 agaactataa aggttatcaa aacagcgttt atgactaaat gaattagagt tatctatcac
     6181 tatttttcaa gcgatttaaa ccaaatggca tacaatatgg catacaaaaa cttcaataag
     6241 ccgtgggggt ttttaaattt cttcttgttc tttaaaatca atgagtggag ctacataatt
     6301 tgaatccact ttttcaaacg tcactttttg catcgacgaa tgttttcttt cccaagataa
     6361 cgtaaataga tttgactctg gacttataaa gtctacttca ccttctagta ttactttctt
     6421 atgcaaatat tcctctaata aatatagact tgctaaagca tacaacacat tttccaaatt
     6481 tgcttcctta taatttgaag aacgaccatg ttttactctg ttatatattt tccaccaatc
     6541 tggtgtatct tcaggccacc catctccaaa aggttttagt gaagtcaatg ttaaagaatt
     6601 cataacccta acaccattat ccaaacacat taattcgcta gatttaataa cagctttaaa
     6661 atcagttatg cttttattgc tactgctatc aaatccgcaa agttctttga aaataatctc
     6721 acactccgaa ccaatcgcta gtagtagttt ttgatattca atagagaatg ttttgaaatt
     6781 attctcatcg agcatcacaa aatctgtggt attaataaac tcattttcta aatgtagata
     6841 atacttccaa taaaaatttg taaactctgc tttgtccatt aaatcacctc ttttgaatta
     6901 taacaaaaaa aacacgtcca caacagacgt gttctttgat ttctacacaa tcagcttgcc
     6961 aaaaccatcc aatcagtcct agcatgcctg tattaaaatt gaacgttcat catctatcat
     7021 ctttcttagt ccagcatgtt tgcaaccaca cctacaattt tcagtccaac aatagatttc
     7081 gactagctaa gtcttgcaaa attcaggcag ctaacccttc atttactacc ttaattatac
     7141 taaataccca caacgtttac aagtgtaatc attctaagaa atctgtttag ctatgatcgt
     7201 tgtataatct ttaaacgccg ttccttcatt gattgaaata attttataac tcttgttttt
     7261 ccaagctact ttcatatcgt ttgttagttc ataatcttga tcgtatcgaa taataaacgt
     7321 caacgttcct tcaagaatgg tacctacact agcaagcacg tcttttagca tttgtgaacg
     7381 cacattagcc caacattcaa atacagtttc ttcactatcc accatctgcc catcttcatc
     7441 ttttacccgt tttgtctttt taaacttgac tctctcagtc aattcattta cattcttaat
     7501 gattggcata ataagcacct ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc
     7561 gttcgctgct ggcttcttga cggttcaaat accaatgttg agtaagtaat gacaccgcca
     7621 aatcaaattg ctgataatta tccatatctt tatccgttgc gctactatca atagcacttt
     7681 ttatatattc gcctgctgta tcaatcaatt gttgaataaa gtcatcatca atagtgtgat
     7741 cgatccgcat actttttttt atgctatcca aaattaccat tttatctctc ctaatgaaaa
     7801 aaaggcagtg ggataaaccg ccgcccaata tttttctata cttttggtgt tacaactggc
     7861 gtaaactcaa tatacactga agcattttga tcaattttct tataatcgtt gcgaacgatc
     7921 actgcaagtc cttgtgagta gtaatcgaat ttttcccatt gagtcgttac ttgatttcta
     7981 cgagcaacaa agatagattg tgcaatatcc cccataatca tagggaatgt tccagcttta
     8041 ggattggcaa acaatgtatc tgcaatcaat actactggca taccgaataa tgatttacca
     8101 ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat ctgaatcttt caatgtatct
     8161 aggtggtaat atccgctttg gtttagaatt accattttat ttaatgctgg gtctaatgcc
     8221 acattgtata gttgtttcaa atcgtccaac gtagcagccg ttttcttagt gaatgttttt
     8281 aataaatcca tgatatgctt attgtcagtg ttttctacca atttcgctaa ctgatctttg
     8341 acctcttgta caatattcac tgctgaatct tccacaacct catttgataa ggcaatctta
     8401 ccagcacgag tttctacttt ataatcaact gaagtaaaca tttcagcgtc aatatcacca
     8461 atttcagcaa gttcagcttt tgttgctaac actgcttgtt ggttagttgc tactggatat
     8521 ttaccttgtc catttgatac tgtttttact gttgcatatt gagctaagtt ataatttgaa
     8581 cgtttcaaat caaagacttc accgattact tcttcgggaa caactgccgc tgcatttaca
     8641 gtagtgactc catcacgcac ttcgccttgt gaacggatat attcttcata cccacgcaat
     8701 tctgtttttt cgttatcgat taaagttttt tccatgttat tactctcctt ttgtgcttta
     8761 gaagtggatt ctaagctatt gtttgtttgt tttgcttggt tgttactcat aaacgattcg
     8821 taagaacgct tatttacttg gacatttgat gaatcataag cgggaacggt cacgacacta
     8881 atttcattaa gtgctttgat tttatctatt gatcgttcaa ttgtgccatc ttcttttttg
     8941 tcgaatgaat ctaatccaag cacgaaccca aaggacatag aatcaacaac cccttttgag
     9001 atattctcat acacatcatt tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat
     9061 cctgtatcat cgacattcaa tttcaacgta cctgctttga cactagctag tggctttgaa
     9121 taatcgtgtc catataataa gaacacattt gataaatcca cctcttttaa tgcttcgggt
     9181 gtaatcactt ctacaaagcc gcctaaatct tttgacggtt gcccgaattt caacgcatag
     9241 cctgaaatag ttttgccgtc tgttggttgt ttttcatttt ctttctctac tttgggagaa
     9301 tcggctgtta gctcagcttc ttctgtcagc cgtttttcct tttcttcttc attattcaac
     9361 tggtgtaact ccttcctgtt cttttaattg atatttctcc aacgtatcta aatacgtaaa
     9421 gttcaaactt gctagtaaac gatcgccacc atccatgggg ggtagcccca ttttcgaccg
     9481 cccctcatta atcgtgagaa gtgacccttt gacctgatcc aatacatttt ttacttttgt
     9541 ttcaggatca gtctctagta actgatcgga attaaagcgg atattggttt caagtttcgt
     9601 atctaattca ctcacaaaca cattaaagta atgaatcagc gtactttgga tatactggag
     9661 attactttga acggtgctag aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc
     9721 aaacgccttg gcaatttgtt ttgtgttcca atcattggaa ttgataagct tcaaaacatc
     9781 ggtgtttatt tctaatgttt tgtagtccat tgtttcatca agaatgatcg ttctaagagc
     9841 attatctcca ctacttgaac cgttggcttc ttcaaacttt tcacgaattg cgttctttgc
     9901 tgatccgtct aaatctgact tatgaacctt caaaatacct gacccactga caccacgagt
     9961 aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc
    10021 ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc
    10081 gctagacttc acacgtcttt ttttatcacc aatctgataa gacaacgtac cattatctaa
    10141 ttgagtaacc gttacacttg agttaggtaa taagtgaatt tcttctacct tttcaccgtt
    10201 gcgtttaatc tctgcaaagc tattaccgtt tagcaacata ttgaccgcca aagcaaactt
    10261 taaatgccaa ccatccattt ctgaattagg tttctcgttt agtaacttca ccagttcatt
    10321 atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc
    10381 aataattcga acggctgcaa acacatcact atttcttaat gcacgaacgc ttgtaaagct
    10441 ggtggtgtaa tcatccgatt ggattgatac cacatgttgt aggaaaggat ctcccgttgt
    10501 attttgtctc gctttaaaaa atgccatctt ttaacctccc ttctgttctg tatttagcat
    10561 gaccgctacc ccaataagca ctatgcccag cgctaacaag ccatagtaga catttgttag
    10621 taaggtgata gcaaccaaca cacacaccaa tccaagaagt aaaagaacgg tatgaatgta
    10681 attagaaaga aaattcttca cttgtataaa attcattgtc tgctttcgct ccttccgttg
    10741 tggtgaagta atccattgca aacacatagg cattgattaa tgcagctatt ggatcaattt
    10801 tgttactgtt ctttgattta ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt
    10861 tgttcactgc atgggtgaga atcgtatttt tattgtgaat aacgttgcct tcataaactt
    10921 gttctctaaa agttcgggtc ggaacgttta gagtaatcgt tccttgtctt acttctaaca
    10981 ttgggtagtt ggctttttca gctttactga taagcgaatt agcgttatac gggtcataac
    11041 aaatccccat acattccaaa tcattttctt gaatcaaatc ttgaataaac tgaaatactt
    11101 ggtcatagtc cacgattcca ctttctaatt gagtaatgga acactcacca gctttttcta
    11161 gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg aagcccatat ttggtggcta
    11221 caaagctatg tgaatcacaa taaagttcgc cgttatctag cggtacgatc catgaaacgc
    11281 tagttaaatc atctgtttta gataaatcca ctccgatata aacgggcttg cctgtaatat
    11341 cttgtggttc tacttcggtt gcctgccagt cttcagcaat catatagctg tcctcactcg
    11401 cttgtctcca taaattgaag tttttcacca atacagaatt aaggttattt tgtttcaaag
    11461 caaggttcac atcatcttga atggttggaa tcattacttt tttgatttct tcactttcaa
    11521 aaatcggatt ggctttgatc catttctctt gatcgtgaat ttcttcggga tcgtccaatt
    11581 cccatatcgc tataaagtac ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca
    11641 ataagtattc ctcgtacata ggaacgttta aatcaagccc agcggtacta ataacacaca
    11701 ataagccgtt cttttgttgg gtcattcctg acttaatgac gttgtacgtt ttacgagttt
    11761 ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc aaacccatct agcgtgctgg
    11821 tttcactagc cagtgccata gcgaacgaat tagagggcaa gtgctgaatc ttagagttca
    11881 taattttcag ttgtggtctt aaaaacttac tagacttgac cacgtttcta agtgaattag
    11941 acagcatatc atagcccaat ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc
    12001 ttgcttctgc tggttcttgt tccataatca acgcattggc agccatgcca cttgcgatat
    12061 acgttttccc attcttacgg ctcatactgg taaacgctcg attgaatcgc ctgtaattcc
    12121 cttctttagt acgccagcca taaagactgc ctagaatgaa tttttgaaat cctaacatct
    12181 tgattgcttt accgtccgta gatggtagca actcaatgaa tttaatcgct ttggtggctt
    12241 gttcttcatc gaacacataa gggaagctat ctgacttaga acgctccaaa tcgcttatat
    12301 gacgctcaca cgcttggata attttcttgg gtgcaatcag cttacctgat aggactttct
    12361 caatgtaatc attcatgaat tcatcaactc tttgaacggg tcttcgggtt catcattttt
    12421 aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc atacctagtg aggtggcttg
    12481 ttttttcaaa tcactcatag cttgtgattg aacccgaacc gctggattgg ctttctttac
    12541 tccgttttcg catgtgataa acgtacctga ttttcttatc tcggcttcgg ctgttttcat
    12601 tcgagcaaag gctaaacaat aggctaccaa tgaaccataa tctgcttcac tcaatggata
    12661 atcttttttt aatgttggta cgatacgatt ccattcactt ctagcgctcg ctgccatcca
    12721 gtcagggggc gtgatattta ttaatggttg ctgcttgaac aattcttctc tagcgtccac
    12781 acggattgct ttttcttcac ttgatatatt gccttttgta tcgtgtaata ttttagggtg
    12841 tcccatgttg tcctcctttc agacaaaaga aaaaggaacg taagcaatta agctcacatt
    12901 ccttcgtttt tcgatcagaa tttttcttta tctaatttat ctatataaat gatttttccg
    12961 ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct
    13021 gttttatcag taataacaat tgtatttttc aataaatctc ctactttcat tatatacttt
    13081 ttcacttgtg attacaagtg taatcgttat ttgtaattac ccttatgcct tttcacaacg
    13141 gttatttgta ccaaaatgag taggcatcga ttgtcagtaa gtttcacact atgccccctt
    13201 aactcaaagg gtactctttt ctcgtctttg tctttcattg gctgtcttgg aattatggtg
    13261 tgcatggcat aaagaacgaa aattagtttc atctaaccgt ctattccaat catcttttag
    13321 ttcaattttg tgatctacaa catcagcttt ccttatcaag ccttcttcta ggcaatcctc
    13381 acatactgga tgatttaacc gatacaagta tgaagccttt cgccatgttc tactttgata
    13441 gaattgaaaa tatttacctt cgctttgctt tctttcttca cgcttaattg ccgtttgctt
    13501 tgctttatgc ttcccacaat acttctgcct ataatcaatc aatgttttac atccagcatg
    13561 attacattgt tttttaactg ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc
    13621 atcttgttcc cattttgatt tgtattcaat ataaggcgaa actatcttca agcacgttcc
    13681 gtctttaaaa ttaactttag tgattttatc ttcctcaaaa ctaataaagt ctacatcttc
    13741 ttgagtgttt gaatactcaa tgtgtttctc accatcgaag tatctaatac tttccatatg
    13801 cataattatt ctacctccaa taaattagtt atcaattcat catctgttag tagcaaagcc
    13861 aagaacttat catggatgtt taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt
    13921 ctcgctcgat catataggtt aagcagacaa ttaaattcat ccattagctc acctgtatca
    13981 tcatagttgc cactatctac caatgatccc actactagcg attgacacca cgctactgac
    14041 tcctgcagcc aatcaggttc ttcttccatt atctttaata cttctggatc aaatgtggaa
    14101 taatcttctg ttaccaatcc cacactaaat gccagcgtgt tcattagaaa ccgatccatt
    14161 tcatctgtat attgaaagtt cggtgctttg tttgttgaaa taatattgag catttacttt
    14221 cctccttgat tcgtaacacg tggtaacacc tacgtaacag gtacccgtta ccactcaatc
    14281 ccaacgggga caagggttct agcgttttgg taacaggtaa cgggtaaaat ccagtaacat
    14341 tattccccag catattagct aactatttca aaagttttaa ggaaataagg tgttaccccg
    14401 ttaccaaatt gctgtatccc ttggggcagt aagggtaaac tagtaacggg ctaggtgtta
    14461 ctgacccgtt actacccgtt actttttgta ctttccagct cgatttttat tttcataggc
    14521 atttgactta ttgtaaaatc ctcgtcgttt atttcttttt ggcagttgct tccgttccca
    14581 accaggaata ctgctaataa tcaaggcaat ctttttagtt tctgcatctg atcgaccatt
    14641 caataaatcc tttgcttgtt tatcaaaaac agcttccaaa atatcagcgg tgagtacgct
    14701 atcaactaaa taaaactctt gttgcatagt agctccatta tagaaatgct gtaaattttt
    14761 ctcattacat ttactgtcag gatagttctg ataataagaa cgtctcgccc acgctggagc
    14821 ttcttcccat ttatacggga tttccatttc caaatattta gtgattgctt ctttaattgg
    14881 attctccatc ttggcatcct cttgatagtc ttttgcaatt tctaagactt ctttatcttc
    14941 atcgggacta aaatagattc gttgtccttt atcaaaaagc acctttgctt cagctaacac
    15001 ttgaagaaaa tattcatcct ttgttttaaa cggattcatc tttggcttat tcttacaagg
    15061 caaaggataa aaacggcggt ttcctgtatc atctttcaaa tactgcccat cattcgacgt
    15121 tccaataaat acacattgcc ttgcccaagc tatgacattc cgaccataag gaggtctaat
    15181 tttatcgatc ttggcactga tgaagttttt tatctgttca attttagttt tgttcattga
    15241 agataattct ccaagttcaa taattacatt gccttgaagc tgcatataat catctttctt
    15301 ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac
    15361 ggtactctta ccaatccctt gcggtccatc caaaactgga acgatctcaa actttattcc
    15421 tggtttatag atacgtgcga ctgatccgac tatccaacgt tttgttactt ctcgtgtgta
    15481 aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt tccagtctag gctgttgatc
    15541 ccatttaact ttttcgatac gttcttttac tgggttgaaa acattctttc tagccacatt
    15601 tactactgct ttgtgtattt tgttgtcatc aaaaactaca ttgtagtttt cttcaatata
    15661 tgccgttaag gcatcctcaa aactgtcttc ccattctcca gcggtacgat tgatccaagg
    15721 agaatctttt aataggtgaa tgtatccgct aaattcgtta taagcaattg ttccagctaa
    15781 attgctatca ttggttaaaa tcaattctgc attcacaaca ctgttacttt taatctgatc
    15841 gtctttattg catcttaatc gtgacatcca ggttttcaat tcaatgatat tgtcttcacg
    15901 ctcgctggct tcgggaaagt ctaaaatact atctaattta tgcttatgag ccatgagctt
    15961 tccccctcaa atctttttta atcatcgatc gaataatttg attcacttct ttatctttta
    16021 gcggtggttc attgtacgca tttgctgtat aaatcaattc tgctaaagtt tgattatcta
    16081 caccgtgcca tagtaaccaa ccgcataact tagttaaatg atcgtttcta ctaccttcgc
    16141 cagcgccttg aacaatcata tctaattttt ttgctgtccc tgtttttgct acgggtcggt
    16201 attgagtctt tatttcttgc ggttttttta gcagtaggtt cactagccaa tcgggagctg
    16261 gtgcaatatt atttagagat acgttttcaa taggttcata tgctctccca acacttcttg
    16321 aaataatcat agttggcgct attggaactc ctttagtgat aatatcaatc ccgcttaaac
    16381 ttttaccttt atcatcacgc ccgaagatat cagcaaatgc acctgtttct tgttggataa
    16441 ttacaccatc aggaatttta aagaagaaat ggattccttt acctggtgtg atttccgtat
    16501 aagtagaagg aaatggatca tacattgaag caagtttttt ataattctca acgccgtttc
    16561 gtgtttcaga atgttgatct aggtcaacta ccaccatttg atgatcttca agcattaacc
    16621 caatatttct agttgcatct tccgtccacc attcattaat ttgattagaa tcgttagttg
    16681 catcattcca accactagag ccttttattg gaattttagt tccaggagat aagggataaa
    16741 ttgaccatcc cgacttttgt aattccgttg ctgcttgata attatttgcc aataaacctc
    16801 tcctcctttt tcaaaatact ggtataaatc attcagacac ctccatgagc ttcaactgct
    16861 caagttcgta tttcaaccca tcccgaacaa attcagcacc ttttaattga ttctgatact
    16921 ttacccatgt attaaatgtt ttcacccttg cttcttgcat atcagtaatg tttaaccatg
    16981 cggttaaaat ctgatttttt agctcgttga tttctcgaca aattattttg tgttctgcat
    17041 gttttttgag tacatacaaa actgttagtt tttcattgaa tgaatttcca aagtaggttt
    17101 tcccgatatt attttctagt tcagcgatcc cttgattaat ttcctctaat tcaaggctat
    17161 cgagttggtt taatagtttt gttttgtaca tctacttatg ccctccagtt cttaattgat
    17221 agtcgtactc gtcataagca gtgaagagat ataacgctaa tcctgcaaag atagctattt
    17281 ttgcaattaa tggtaataag ccgaaaattc cgccgattaa aatgcttcct ataattaagt
    17341 ttcgtttcat aatggttccc tccgattttt taatatggta taatcggaac aaagaatatc
    17401 gggttttatc ccaattaagt tgcctttatc ctgtccgcca agactgttat ggataaggct
    17461 ttttctttgt tctcgatcat gttaatgctc ctaagttgtc tgcaattcag cagaacgatt
    17521 ataaattttc tcactcactg aaaactgcaa cccatatttt tctttaacag tcattaattc
    17581 tactgtctca tctaaaatag gttcacgatc aaccaacatt tcaggagtca tatctttctt
    17641 ttttaccatt ttggaatgac cataaatagt tgatacagct ttattggcta tcacattcgc
    17701 tttaatgtag tctactggtt ttggttcagt aatagcgtgg cttaatttag tcattgcttc
    17761 tttttggtgt tctttgtcta gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg
    17821 gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat gcctcggctt cttttttacg
    17881 gcttttgaat gccatgcggt aaatattttt tttcgttgat gatgtttaca tcttgtgttc
    17941 tgcccaatga atcgatgacc ttactagtag taaccccatc tttaggcaaa gagtgaatgg
    18001 ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc tttcgccact gcccaccatt
    18061 catcattaat atcgacaaat cttataatgt gtccgttcca aatttcagtt ttcattcaac
    18121 gtcctccagt tcaagcccta gcaagctcgc taacaactca acttgctgat acaaaatttc
    18181 ttgatactct tcaatagaaa gtcgttcttt aaactctgct tgtactaaat cgttcaagcc
    18241 acgcaatact ccgcttaccg ttggttcttg tggtagcaat ttttgatagt ccatttgttc
    18301 gtacaaacat tcaaacgttt cttctagcgc tttgccttcc aagtttgcta attctgccgc
    18361 tgtgtcttct gtaagaattg ttgataatac tttttcgatt gctttatttt gtgtcgattc
    18421 cattattatt tcctcctatt aatccctttg cccccacggc ttgcgtttat ttttggtatt
    18481 cttccataaa gcgatcacat tcatcttgtg aaattcttac gactccatca actataatca
    18541 actttaaacc gttaggaaca tatttctgca tcatagtatt gtatgaaacg ttcatatact
    18601 tagcagcttg tgccttgttc atatattttg gaaactggta ctttttttct aataatgaaa
    18661 gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg atcgtcaata attttaaaag
    18721 ctggatcatc aagcgtgagt ttgtcaattt ttaattccat atttatcccc cctcgttagc
    18781 atacatttct atacaaagat tttaaaaaaa tgcaagaggc tcttgaatac atttctatac
    18841 ctcttataaa ctcaatatac catcatacct tttcatttgc aagaatttta acatactttt
    18901 ttgaactttt atgtttcaca catatttaaa tcgtgatata gtagagaaaa tatatgaatg
    18961 gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt ggagacagga caaagaatta
    19021 gactggctag aattgaaaag ggactctcaa tggaacaact tggaggtaaa ttatccccac
    19081 cggccagtaa aggtgccgtt agtaattggg aaaatggcta taatcttccc aataacacca
    19141 ggcttaaaca actttcaaag gtattagatg tatccactac ctatctatta aacggatctt
    19201 atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat
    19261 tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct aatgctatca
    19321 ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca aacgcaataa
    19381 aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca attgcttatc
    19441 tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat tcagaaaaaa
    19501 atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta aaagagttca
    19561 cagctcttgt tactgagcta aataaacact attcaaaata ataataaact gcctcctcca
    19621 accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca ttcaagcaat
    19681 atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg aacaaaatga
    19741 ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg
    19801 ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg aacaacgaaa
    19861 cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc cgagaagcca
    19921 ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat ggaaatcttc
    19981 gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg gctgaaacta
    20041 ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac gctatcaatc
    20101 ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga accaccacta
    20161 agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg tttgattacc
    20221 taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta cgctttctct
    20281 tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac attgattttc
    20341 aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc tataaaatca
    20401 gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga acaatttcta
    20461 tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt gtaactgatc
    20521 cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct atttatgcaa
    20581 gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat gtagcaagac
    20641 acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt caaactagat
    20701 tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca aaagaaacga
    20761 aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg gcatacaata
    20821 tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt t
//
LOCUS       DACGLH010000042        20586 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.045, whole genome shotgun sequence.
ACCESSION   DACGLH010000042 DACGLH010000000
VERSION     DACGLH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.045"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(577..1593)
                     /gene="tsaD"
                     /locus_tag="IXT21_001753"
     CDS             complement(577..1593)
                     /gene="tsaD"
                     /locus_tag="IXT21_001753"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ4869142.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1590..2054)
                     /gene="rimI"
                     /locus_tag="IXT21_001754"
     CDS             complement(1590..2054)
                     /gene="rimI"
                     /locus_tag="IXT21_001754"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ4869143.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2061..2603)
                     /gene="rimI"
                     /locus_tag="IXT21_001755"
     CDS             complement(2061..2603)
                     /gene="rimI"
                     /locus_tag="IXT21_001755"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ4869144.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2587..3411)
                     /gene="tsaB"
                     /locus_tag="IXT21_001756"
     CDS             complement(2587..3411)
                     /gene="tsaB"
                     /locus_tag="IXT21_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ4869145.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(3500..4480)
                     /locus_tag="IXT21_001757"
     CDS             complement(3500..4480)
                     /locus_tag="IXT21_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4869146.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4504..5988)
                     /gene="galT"
                     /locus_tag="IXT21_001758"
     CDS             complement(4504..5988)
                     /gene="galT"
                     /locus_tag="IXT21_001758"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ4869147.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6000..6989)
                     /gene="galE"
                     /locus_tag="IXT21_001759"
     CDS             complement(6000..6989)
                     /gene="galE"
                     /locus_tag="IXT21_001759"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ4869148.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7237..7404
                     /locus_tag="IXT21_001760"
     CDS             7237..7404
                     /locus_tag="IXT21_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ4869149.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(7466..9277)
                     /locus_tag="IXT21_001761"
     CDS             complement(7466..9277)
                     /locus_tag="IXT21_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ4869150.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(9274..9639)
                     /locus_tag="IXT21_001762"
     CDS             complement(9274..9639)
                     /locus_tag="IXT21_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ4869151.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9802..10197)
                     /locus_tag="IXT21_001763"
     CDS             complement(9802..10197)
                     /locus_tag="IXT21_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ4869152.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10215..11177)
                     /locus_tag="IXT21_001764"
     CDS             complement(10215..11177)
                     /locus_tag="IXT21_001764"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ4869153.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11177..11389)
                     /locus_tag="IXT21_001765"
     CDS             complement(11177..11389)
                     /locus_tag="IXT21_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ4869154.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(11410..12108)
                     /locus_tag="IXT21_001766"
     CDS             complement(11410..12108)
                     /locus_tag="IXT21_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ4869155.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12128..12670)
                     /locus_tag="IXT21_001767"
     CDS             complement(12128..12670)
                     /locus_tag="IXT21_001767"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ4869156.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12802..13806)
                     /locus_tag="IXT21_001768"
     CDS             complement(12802..13806)
                     /locus_tag="IXT21_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ4869157.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13803..14792)
                     /locus_tag="IXT21_001769"
     CDS             complement(13803..14792)
                     /locus_tag="IXT21_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ4869158.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14789..15595)
                     /locus_tag="IXT21_001770"
     CDS             complement(14789..15595)
                     /locus_tag="IXT21_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869159.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15761..16717
                     /locus_tag="IXT21_001771"
     CDS             15761..16717
                     /locus_tag="IXT21_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4869160.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16794..17312)
                     /locus_tag="IXT21_001772"
     CDS             complement(16794..17312)
                     /locus_tag="IXT21_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4869161.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(17400..17549)
                     /gene="rpmG"
                     /locus_tag="IXT21_001773"
     CDS             complement(17400..17549)
                     /gene="rpmG"
                     /locus_tag="IXT21_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ4869162.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            17777..18223
                     /locus_tag="IXT21_001774"
     CDS             17777..18223
                     /locus_tag="IXT21_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ4869163.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(18418..20313)
                     /gene="asnB"
                     /locus_tag="IXT21_001775"
     CDS             complement(18418..20313)
                     /gene="asnB"
                     /locus_tag="IXT21_001775"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ4869164.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6142 a         4399 c         3554 g         6491 t
ORIGIN      
        1 tatcagggtc actgcttatt ttcctcccta aaacttctga aagatccggc agttttagat
       61 cactggacaa gactcaaaga ttttctatgt gtgtttcagt tatgtaatat tcactgtttg
      121 caaaaaatac tgttagatta actagatgaa gcgtcttgtc ctcatccgat ctagattatt
      181 cgatattcct cgacaataaa cgattactca gtgagggcaa tcagaggaac ggataatgat
      241 aaataagctg taaaaaatag caaaataaca ataatgatta aaagtatgta aaaaataggg
      301 ctttgacaaa aacgtgtcac agccctattt cgattaaacg gtgtgtacct gtcaactctt
      361 cggtcacaag tcataatgtt cataaacatg aggagctgtt ttttgaaatt gttccttgtg
      421 aatcagagtt aaacgacagg cttacgaact cttgattaaa cggcgttcaa aagcttgcat
      481 acgcggtaat aagaacagcc aggtcaaaaa catgagaagc cgtttttcct gggctgttct
      541 tattacttga tgcagggcag ctttttcaca acctctttat tcagataaaa ccaagcttgg
      601 atcagcattc aactgatagc tagcaaaatt tcttttttgc atttccacat gtgctgctgc
      661 tccaatcatt gcagcattat ctccacaaag tcgaagaggg ggaatcacta gttctacctc
      721 agggagttta gctgccaaag catcctgcaa tccttcacgc aacccttgat tggccgctac
      781 acctccagct ataatcagtt gcttgactgg ataattttga caagctcgaa gtgttttagt
      841 aatcaataca tcgatcacac ttgcttgaaa gctagctgct aaatcatttt tgtctagatc
      901 ttctccacgc tgttgtgcat tatgcaccaa attgataaat gcacttttta atccgctaaa
      961 actgaaatca taattatctt catggatcat cgctcgcgga aaatggtaat tatcttttcc
     1021 ctgatgagct agctgatcga tttcttttcc gcttgggtaa gaaagaccta atactcttcc
     1081 aactttgtca taagcttctc ctgctgcgtc atctcttgtc tccccaatga tttcatatga
     1141 tccatcttct tgcatataga ctaattctgt atgtcctccg ctaacaagaa gtgccatcaa
     1201 tggaaactgg aatggtttaa ccagacgggc tgcatagata tgccctgcca tatggtttac
     1261 aggaataagc ggcaattggt gtgcccaagc aaaagcctta gctgcagaaa taccaatcag
     1321 caacgagccg acaagtcctg gtccataagt gacagcgact gccgataaat cttctgccga
     1381 cacaccagct tctaccagtg catcttccaa gcataaggtg atttgttcta cgtgatggcg
     1441 gctagctact tcaggtacta cgccaccaaa acgtttatga ctgtttattt gagaagcaac
     1501 gatgttagat aagatctctg ttccatttct aacgaccgct acactcgttt catcacaact
     1561 tgattcaatc gccagaatca attcttcatt catttcatcg tctccgtttt taactttctg
     1621 ctcataatga tcgcatcttc cgtcggattg cgataatatg atttccgttt acctaatata
     1681 cgaaactttt ctgattcata aagttttctt gctgcttcat tagatagacg tacttctaaa
     1741 aaaacatgca gcgaatcttt tatttgttcc gtaatcagta attgtaacaa atgccgtgca
     1801 tgcccttttc cttgttctga aactgcaaca gcaatatttg tgatctcgac ttcatctatt
     1861 actttcgagt atccaataaa gccacagatc tggttatctt cagctaaaag cagataatca
     1921 gagtgaggct gttggatgtc tgccaaaaac tgcattttcg tccaaggtga accatgtgga
     1981 taagcatttt cgctaatcat ccatacgcat tctgccaagt acgcttcatc aaaatctttt
     2041 tttctgcaaa tcaactaatc tcactcattt tctaaaagtt ttatcatatc caacgcatct
     2101 tcttttgttt cagtgtaata tctttttttg atcgttgttg cttgaaaccc caatttccga
     2161 taaagtcttt gtgcatcttg attactcata cgcacttcta atgacatcgt ctcacagcga
     2221 ttcattatag caaagttctc gatttcgtca ataagcagcg aaccgattcg tttacgctga
     2281 tatgccggtc gaacggcaat attcgtgata tggcaatcta aaccgaacgc tcttgaaccg
     2341 ataaaaccaa tcattcttcc attatcaaag atacctagat atagatgagg aagcggtgac
     2401 ttcaattctg atataaaggc actttttgtc catggcaatt cgccattata gacttctcgt
     2461 tcgacatcca gcagctcttt gatatcttct gtagtcaatt ctcttagtac gtaacgacga
     2521 tcgatttgtt cgactgtttt ttctgtataa atcattttat cttgaaaaaa gcttctggca
     2581 tacgctctaa attttttcaa cataactttc gtctccaggt gtgtgcgtct ccaaccattt
     2641 ttcttctgct tctactcgtt ttaaatagcg aggaagaaaa gcttgtttct ctgcaactgg
     2701 ttcaactaaa gcacctaatt gagccacagt tacaccactt ggaatatccc atgctggaac
     2761 tgtattaatc atcgcactag gcaatatttc ctttatttca gagaagaatt tgtgacaatc
     2821 actcccgacg aaataaatag cttcattcag cttttgtagt tgtgtgaaca aatcagaagc
     2881 agaaatatgt acatcttcaa gtacagtttc taatctattt tctttccagc ggtatgcccc
     2941 agcgtataca ttttttcttc gagcatcaaa catgggcaca ataatattat ccacaccgat
     3001 gcaattagcc gcgatggttt tcagactaga tactcctact agctctttat ctaatgtata
     3061 agcaatcgtt tttgctgtcg ttacacctat acgaagccct gtataagaac cgggaccatc
     3121 agaaaccgcg attcggtcaa tgtcttttgg acttatttga agatctgcaa acaattggtc
     3181 gattgcaggc atcagtgtta cactgtggtt gcgtttgata tttgtttgta tttgtccaag
     3241 aatttgttcg tcttctacca caccgatcgc caaagtttgg tttgcagtat ctattcctaa
     3301 tgtaatcata tatttgatcc tttctgctat gacattctcg ccttccattg tagcatactt
     3361 ttttattctg aggatgaaaa taccaaaggc aaaactagta caataataca tgattatctg
     3421 ctggcgggca aattttgccc cttcctgaaa cacaaaaaag ctgcggtatc actccatttt
     3481 cccacagctt ttccatcttt tatttcgttg attctcgaat aatcaactct gtttccacaa
     3541 taatctttct tgaaaccggt gctttggaat tacttaattg aagaatcgtc tccactgcta
     3601 gctctcccat ccattcggta taaactttta cagtagaaag cgagggcgtt acatatttcg
     3661 ctacactgac atcattgaag cccatcacag aaatatcttc tggtaccact aaaccttttt
     3721 cttgaatcgc tttcagtgcg ccaatcgcta atgcatcatt agctgcaaaa agcgcatcag
     3781 gaacatgcgg attcgttttt aaatattgct tcactgcttg atatcctgac tcgacagaaa
     3841 aatctgcttc gataattgga ccttcgtcgt agcctttttc tgccaaaact tgttgaaatg
     3901 ccataattct gggatctttt agccgctgat ggtttttctt cgtatattct gttccagcaa
     3961 gcatagcgat atgttgcgct ttatgggaga caaagtgttc aatgactttt tgaacacttg
     4021 acttaaagtc gacaactaga gagtcgattc cttgagaagt gccgtcagaa tctaccaaaa
     4081 gcaaggcatt ttgggatcct ttcatttcta ataactgagc ttcgtcgaat tttcctagtg
     4141 cgatcgttcc atctgccgga agatcactaa tggaatccca cgtttctttc aataaagtaa
     4201 cgtttgcttc ttctgccttt ttttcgattc ctaggcgaat agataaataa taaagatctt
     4261 caagctcttc cgtatcgtcg taccactgga tcaggcgaag cgtttgctta gtatttgctg
     4321 catttttacg atgtttcgta taatttaagc gttctgctgc ttcaaaaatt ttttgctttg
     4381 tggctaagcc aacggaaaga tcaggatcat agttgagaac tcttgaaaca gtagcaggag
     4441 aaactcctgc caactgcgca atgtctttaa ttgttgccat ttgtctctcc tcctttttta
     4501 cgtttataaa atcgcagtaa atcgttcaaa cgctgcctgt cctttttcat ctcgcttgaa
     4561 tactccagcg tcttccagta cacgtgcaaa aacctgacct acagattttt gtacgacatc
     4621 ggtgaccgta tctttcgtca aatcaggatg ttcttttttt aattgttctg cccacggacg
     4681 atgatattca gccatctggt tttcttctcc taataagaaa tgctctactt ctactagttc
     4741 tggtttcaaa cgaggtggta aaacagcaag ccccattact tcgatcaacc caatgttctc
     4801 ctttttgata tgttggacat ctttatgtgg atgaaaaatc ccatcaggat gttcttctga
     4861 aacattgttg tctcgaagga caaggtccaa ctcaaagtaa ttgtcccttc ttcgtgcaat
     4921 tggcgtaatc gtatggtgtg gtgtaccatc tgctgaaaaa gctctgatag atactgtttc
     4981 atccgaatat gctttccatt tttccaatac tgagttagct gcttcaataa gcaagttttt
     5041 ctctgttgat tgcaaacgga tcacagacat cggccatttc acgactcctg cgatgacttg
     5101 cggataatct tctagtttga taagtttttc tatcggtgct tttgccatag gaaattcatg
     5161 acgtcctgcc tgataatgat catgagaaag aatcgatcct ccaacaattg gtaagtcagc
     5221 attcgaacca acaaagtaat gaggcaagac ttcagtaatc cgaagcagtc tagcaaatgt
     5281 ttcctttgtg attttcatcg ggcggtgttg ttctgataaa atgatcgaat gttcattgta
     5341 ataagcataa ggggaatatt ggaatcccca gctttcaccg tctagattca ttcgaataat
     5401 ccggtgattg gtgcgggctg gatagttcaa gcgacctcta tatccttcgt tttccatgca
     5461 aagcatgcag gtaggatagt ctacttttac cgcctcgcgt tctgctgcga tttgttttgg
     5521 atctttttct ggcttagata aattgattgt gatttccagt tcaccatact ccgactctgc
     5581 cggaaatttg atgttttttg cgatggctct tgttttgatg tagtcatttt tcttacttaa
     5641 attaaagaaa tactcagttg cttcctctgg gcttttcgaa tagtgctgtg caaagaatgc
     5701 attgacgact gaaggtggcg gtgtgagaaa atccattaat tgcgcttcaa aaatctcttg
     5761 ttctgctggc aattctgaaa gaacaccatt ttctcttgcc ttagcgacaa gctgatccaa
     5821 taagtctaca gattcttcct taactggtct tacttcaatg ctttctagag catcttctcc
     5881 taccatccct aaaagacgat tctgtaaata taagcggtcc atttccatcc atccgccagc
     5941 ttcgatcgca agtgtggcaa aatcacaaat cgtttgactg attgacatat ctcattcccc
     6001 tattcttcat aaccatttgg atggctgaca tgccaatccc atgctgtttt gataatcgct
     6061 ttgatatctg tgtattcagg tttccagccc agaatctctc tcgctttttc acttgaagcg
     6121 actaatcggc taggatctcc agctcttctt ggtgctattt ctgctggaat ttcttttcct
     6181 gtcacttctc tagctgcttc tagcatttcc ttgactgagt atcctttgct gctgcctaaa
     6241 ttgaagaagt tgctttcatt tccttctttc aagtattcca aagctaaaat atgtgcagca
     6301 atcaaatctt ctacttgaac ataatctctg atacatgttc catctggtgt atcataatcg
     6361 tctccataaa cagccaaggc ttttctttga ccaagagcta cttgtaaaat gattgggaca
     6421 agatgcgttt ctggtgtatg atcttctccg atagatgcat ctgcttttgc tccggctaca
     6481 ttgaagtagc gcaaagcgac atagcgcatg ccatatgctt ggtcacacca cttcatcatt
     6541 ttttccatca tcaatttact ttcaccatac ggatttttag gattagctgg tgtatcttct
     6601 gtgattggag actctttcgg ctctccataa gttgctgcag tagaagagaa tacgatttta
     6661 ttcacgttga actcgtgcat gacttcaagt aagatttgca taccgtatac gttgttgttg
     6721 aaatacatca atggtttttc gacagattct cctactaaag agcttgcagc aaaatggatt
     6781 actccttcaa tcggttcctt ttcaaatacg gaacgtagaa attctttatc ccggatgtct
     6841 ccttcataaa aatgagcatc tggatgtact gcttgtttgt gacctgtgag caaattatct
     6901 accacagcta cttgataccc cttttgaact agctggtcta cagcgtgcga accgatataa
     6961 ccggctccgc ctagtactaa aattgtcata aaaaacactc ctttgattag taaaattgtg
     7021 tttgttgatt ctatataatt taaaaaacga agtttacttt tatggtgaaa acaaacaaag
     7081 taaaatttcg taaattcata tggtaaattt ataataaaac atttagtaaa cattttcaag
     7141 ggttttttgc aaaggtttac aaacaaacgt aaaactagcc tttttttctc ttttgttcca
     7201 taatagatat aacaaataat aaatgaggtg gattcaatga aaaaatttgt atctggaata
     7261 ttagtaggaa gtctggcaac agccgctgcc gttgctggtt tggttgcttc tgtgaaaaaa
     7321 acagtgatcg atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg taaaaaagca
     7381 atgcgtaaac gcgtttctcg ttaataatta tttaaaaata cgcatgaaga ctttctaaaa
     7441 aacagataag tcatcatgcg tatttttatt tgtcagtcaa tataagttgt gctattttat
     7501 ctgcctgtct cagattccat ggatctgtat aaggtctagc tactgctgca gttagccaat
     7561 ccacaccttc aacaggtatt ccatagcaat aaaagtcaga ctggatttcc cctgaagcat
     7621 caatcacgcg attcgtacct cgctcaacat ctatagcttc tgaatgataa cttcctgtat
     7681 tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt atgacaggat
     7741 tacttgaacg agaaaagctt gtttgtggca atcgagcttc aatcaaatag cgactgctga
     7801 tttctttatc ttgtagagaa ataacatagc ggttgtgctt ttgctgaata tcaaatggcg
     7861 gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc taattctttc gtccgaatga
     7921 ttggcggacc gatcgttaaa aagctattta gatgagtaaa ccatccccat agtatttctt
     7981 tgtattcctt agcagaaaaa acgtgccatt caatagcctg ccggattgga tcacgccagt
     8041 cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt tccgcttctt
     8101 ttatctgccc ttcaataaat attcggctag cttctgaaaa gggctcattc tgataggcaa
     8161 gaggagtttc taaatacgac caatcccatc tataagggat tagtgctgga tgctttccga
     8221 gacactcctc tgaagagagg gagagaaaat catgctcaaa atttttcttt ggaatcggca
     8281 agtgatattc ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt ttcagatagt
     8341 caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca ttgctccagg ttttcttttg
     8401 ttagtaaccg tggccatgcc attgctccgc cttgcttttg atttcttcct cgagggtaat
     8461 aagggagccc ccttcttgat ccactgtata caatcggttc cttgcctgat ggatgataga
     8521 ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga tactgtaaac aatccgacat
     8581 aatcaaaaaa acttaagcct agacctctta aaaagacatt ttctttaggc ggtatcttcg
     8641 aaagatctac atctgcagga ttagaaggaa gctgatacag taaaacattt ttctttgcgt
     8701 agtctaccca cactctttct tcttctgtct catcattttc ccagtgaccg cttgctaaaa
     8761 caagctgatc gatagaatag ctttgctgat ctgtctgtaa aagataacca tcttttcttt
     8821 tgcttacttt ttttactaaa gaataaacaa actgaataga agctggaaat tcttcttgca
     8881 actttgagaa aaaccagcgt tgatagagtc cgtataaaca tcgagtagat tgctgatctt
     8941 tttccagacg attgatttct tctataatta gttgcttatt tttggttacc gtctgttcga
     9001 tataatctgt agcaggtcct ttactccatt gaaacaagtt gggtccctgt gttaccaatc
     9061 ctccactgga caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca gaattcatta
     9121 ataactccgg tggctgattc aaccgccaaa ctcgacctcc aggaccattg ggatcgaaaa
     9181 gaaaaagttc aagagaatgt ctgtcctgat tattatggag aatcctttct gctacactta
     9241 atcctctagg tccggcacca atgatcccta ttttcatttg catgcctcca tttcgataaa
     9301 ttttttcaat aaaacagcat ggttctgatg ttcatttttt gcaccataaa caaaagtaat
     9361 gactggatgt tctttcataa gttgactgag tttaaaaata gcttgcttct tttcttcttc
     9421 gaaaatcaat tcttgctggt atttctttgc aaatgaagca aatcgttctg gtacatggtc
     9481 aaaagttttg cgtgcttcat tactaggtgc aatttctttc aaccacagat ctaactgcgc
     9541 tttttctttt gaaattcctc gtggccagaa tctatccacg agtattctat agccatcctc
     9601 tttttcagca tcaaggtaaa cacgttttaa tttgatcata gttgtttcct tctttctacc
     9661 ttttcgctta gcttatatat gtagataata gcacaaaata aaaaaaatga acggcacaac
     9721 tatccattgc gccgttcatg tgatcttggt caaaatctaa tgattgattc ggtttttaaa
     9781 gctgtcatac aatcatctgt tttatacttt tttacttttg atttttccag accatccgtc
     9841 atatccaccc ttaagtaaat aaatctcttg ataacctgct ttacgtaatt tatttgctgt
     9901 acggatgctc aaacttttct tttgatcgta aatatagaca ggctggtctt ttcgaataga
     9961 tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa tatgcccagc
    10021 atcaaacaca tctttttcgc gaacatcgat tacttgtgct tttctcattc cttctcgaaa
    10081 ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt actcgtagat agacttccca
    10141 aaggatgatt gcaagcacaa tcaatagcaa aatgatatta attacccata aaaccatgta
    10201 atgcctccgt ttttttaagc aaatttcaag gctaatgagg cagcaccaat gacaccagct
    10261 tcattaccaa gctctgctaa tttgattttt gttgattctc ttacttgcgg gaaagtaaat
    10321 tctttaaaat atttctctac acggctacgt aaaaattctc ctgctgccga tactcctcca
    10381 cctaacacga tacttgacgg atttaatgta ttccccaagt ttccgcaagc taaacctaag
    10441 tagaagcata cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg agccatttca
    10501 aagatatctt tacttgttac ttcttctccg ttgtctagca ttgctttcag ttttgaatcc
    10561 cctgcgtatt cttctgccaa gaaacgacct aggcgtacca cacctgtagc gctagataca
    10621 gtttctaaac atccgcgttt accgcaagta cattcgaacc cttcaggatc cacagtgata
    10681 tgtccgattt ctcctccagc tccgctcacg ccgtgaatca aatttccgcc agcaacgata
    10741 cctccgccaa cacctgttcc tagagtaacg aatacaacgt ctggatcgtt ttctccagca
    10801 cctttccatc gttcacctaa agcagcaaca ttcgcgtcat tatcgatagc gaatttgatt
    10861 cctgttcctg cctcgatcag ttttttcaca aattgaactt ctgtccagtt taagttatac
    10921 gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg gggtacccat gccgattccg
    10981 ataaagtctt cggctttcat attatataaa ttcaaacgat gattgattga ttcaatgatt
    11041 tcaggaacga tatgcttacc atcatccaaa atattggtat cgatgctcca tttttgctga
    11101 atctctcctt ctggtgttaa aattgcaaat tttgctgttg ttccacctaa atcaatccca
    11161 ataatcttct tgtccattac aaatttcctt tctttcggct ttcttcaatt cggtgctctc
    11221 tttttaatat gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg ctatcataca
    11281 gattacgcaa ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc acatagataa
    11341 agatgccaaa ttttttgaac agctgctgta catcatataa gctctccatc tttaagcact
    11401 tccttcatat tatacaagta aaccatattt ttttaaaccc agtaaaatac atattacaaa
    11461 aagaaataca aaaactagtg tagaaagaat cctttggtgt atcgaatagc ttgtctgttg
    11521 tttcggtaga cccaaaacat tccctaacac taatccgcca attagcccac cgacatgtcc
    11581 ccaaacatct acagaggtat ccagaagacc aaaaacaaat gtcaacacaa taaataaaag
    11641 aaaatgtctg actagctgct ggatcgctgg gttatgttta aaatgaaacc ctaagataaa
    11701 caacgcaccg aacaaaccga atatcgaagt acttgcacca ccagacagaa cattcgcttc
    11761 gttaaaagca aagcttgctg tatttcccat tatcccgctg aacatataaa tcaagaaaaa
    11821 acgccaatgg ccatagattg cctcgatttg ctgccccata aaatacagta tcagtgagtt
    11881 cattacgaaa tgcatcagtc caaaatggat gaagattggt gtcactaaac gccaccattc
    11941 attgaagtga acaagataag gaccatacat tccggcataa taagggatct caagtcgcgg
    12001 aaataattcc atcatgagga agaccgctgt ctgtatccct aaaaaagcat aggtccataa
    12061 cggtcgtctt aaccatgcac gcaattttat ttgctgttga taattcatga aaacctccca
    12121 tttcatttta gtcaataaac aattgttgga tcttctgatc aaagctctcc ggttgccatt
    12181 gttcattcaa ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa ttatttaaat
    12241 accggtcgta ataaccaccg ccaaaaccga ttcgataacc ggctctattg tagataatac
    12301 ctggtacaat gaggagatcg atcatctccg gcaaatagag cttctctgat tgtggctcaa
    12361 gaacaccaaa actcgaacgt acaagaacgg actgttcatc catttcataa aacgccatct
    12421 gacgtttcgg caaagtttta ggtacaacta ttttcttttg ttctttttct gctcgttgga
    12481 taaccggctg tgtatccagt tcaaattcat tcgataaagt cgtccctatc acttttgctg
    12541 acttccagta ctcgctggcg aatagctgtt tatagattcg ctgttctttt tcctttttct
    12601 tgccactttt ggccagattt ttcagctttt cgattccttt ccggcgtaat cttactttca
    12661 attggtccaa aaactcaccc ttccttctct cataaaatgg tataggaaaa aaagaaaaat
    12721 agcaatcgaa ctttcaaata aatgcataaa tagaataaaa agaagaccta gaaatgattg
    12781 gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt ttttgtctga taaaaagtgc
    12841 atacagaaaa tatggcgcac caaaaagggc aatcaaaatt cctgtaggta cttccccact
    12901 tggtagaatc actctagcaa ccatatctgc actaagcaga aaaatggaac cgaacaaacc
    12961 gctcagaaca aaggtccagt tattttcttt acgtacgact agcttcgcag catgaggagc
    13021 tagtagtcct acaaaagata aactacctgt caccgctaca ctaccgcttg ccaaagcaac
    13081 agctaacaac aaaaggattt tttgactttt agccacgtta aggcctaatc cttgcgcgcg
    13141 ttcttgacca aatgatagca atacaatcct ctgtgcataa agtcctccta gcggaattaa
    13201 gataaccgcc catggaagca atgcccaaac ataaggccag ctcgcacccc agattgatcc
    13261 cgctaaccat gcattcacaa atccataatt ttccttagat aattggatcg tgcatagcaa
    13321 tgttaatgca gaaaggccgg cattaacggc aataccggac aataagattc gattcatacc
    13381 caaaaagcca tttcgatcat aggccgcaag atagacacag caagcagcta aaacaccacc
    13441 tagaaaggct acaactagca atagaaacgg cagcgaatag tgcgaaaaga aacctaaata
    13501 aagcatcact aaaaatcctg ctccggcatt aatgcccaat atactagaat ctgccaattc
    13561 atttcttgtt actccttgca agatataacc agaaacgcca agacaagctc ctgctaataa
    13621 actgatcagt aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag aagatgattc
    13681 tttccctatg aacgtggcaa aaagctgatc caggtaaata aaaggcgtac caatcattaa
    13741 actacctata gacaggatga caagtacaaa aagcaagaaa tagaacgtcc aatatatttt
    13801 tttcaccgtt gttccctcct tacttgtatc agtaaaaacg gaacgccaat caatgcaatg
    13861 actaatccaa aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa atccgccaac
    13921 ataaccaatc caccgccaac taaaaaagag acaggtagaa cataacgata gtccgttcca
    13981 accattccgc gaacaactgg cggaaccaac agcccaataa atgaaacagg tccgactaac
    14041 gaaacggcaa tggctgctaa cactgcaact agacagaaaa caatcaaccg cgttcgttta
    14101 ggatgacttc caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga tattgctttt
    14161 cctaatgaaa aagaaaccaa aacagcaagt atatataaag gaaacaatac tgctaattgt
    14221 ttccatgtga tattggcagc tccgcctaca aaccaaaaag caagatcctg ctgcagatta
    14281 aagaattggg taagtgcttg gctgatagca gtaaaaaaag aactaatagc tacgccagct
    14341 aaaatcagct gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc aaatacgagc
    14401 aaagttacag caaaagaacc tgccaatgac attagaaaca cagaaaaatc agaagcttta
    14461 ggccaaaaag caaaactaag ggccattgcc aacgaagcac ccgcgttgat tcctagtaag
    14521 cctgaatctg ctaaaggatt tcttgtcact ccttgcatca atgcaccgct caatgcaaaa
    14581 caactcccta caagaaatgc tcccgccaca cgaggcaaac gaatatttcg tatgatttgg
    14641 tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc tttttccaca
    14701 gtcgctgcgc catttgaaag agcaacaaaa aaaataatca aaagaacaaa acagaccaaa
    14761 agcaggagtc tatttttctt catagtcctc ataattgatc ctcctgctct tttggcaaca
    14821 aatcataggt tagaaaaact ggtttatttg ttcttggaag cgtgacaatc gttgcatcaa
    14881 tctcaaatat ttttttcata cactcttttg tgatcacttc ttcaggtgta ccttctgtga
    14941 tcaacttacc gtttttcatt gctaataaat aatctgaaaa acgtgaagca tgattaatgt
    15001 catgaatcga catcaagatc gttgtttgtt tcatttgatt gatttcagtc aataactgta
    15061 ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag atcaagatat
    15121 ctgtttcctg agcaagcgcc atagcgatcc agacacgctg ggtttgtcca ccagacagcg
    15181 ttgcgatatc ccgcatacga aatccagcta gacctgtagc atccaacgcc cactcaattg
    15241 cttcatgatc ttgcgtgtcc atgccactga acagtttttg ataagggtat cgtccgtaag
    15301 atactacttc ttctactgtc atacctgtag ggtgttcact agtttgtgct aataatgcaa
    15361 ttttttttgc aagttctttt gaactgtatg tagagatatc agagttattg acaatgatct
    15421 ttcctgattt cacaggtagt atacgggtaa aacttttcaa taaagtagat tttccacttc
    15481 catttggccc aatcaaacta gtgattttcc cttcaggaat cgtaacagac atgttttcaa
    15541 tgattttttt gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc gtcatttcac
    15601 ccatcctttc gctgaaaatc attctcaatt atgacgctgt ttcaacattt cgtcaagcgc
    15661 tactttcata tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc aggaataata
    15721 accaatgaga aagattctca attatgttag gaggaaagaa atgaaattaa aaacaaaagt
    15781 attcacatta tctagcatat tgctgctggc tggagtactg agcgcttgct ccccctcttc
    15841 agataaagaa acagctacaa ctgccaattc gacgacttca tccaatagta ctcatacagt
    15901 cacagataca cttggacata aagtagagat tccaaacgcg cccaaacgga tcatcggcag
    15961 ttacttagaa gactatctga ttgcactagg ggaaaaacct atcgcccagt ggactgttgg
    16021 cagtggttcc attcaagatt atctgcaaga tgatttaaag gatgtcccaa cgatttctta
    16081 tgaccttcct tatgagaaag tgttaagctt tgagcctgat ttgctgttga tttcttcttc
    16141 cgctactgtc gaaggcggga aatacgaaca atatagcaaa atcgcgccaa cttatgttgt
    16201 aaaaaacgga acagatgtca catgggaaga acaactaaaa gatgtcggca aagctttggc
    16261 aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa aaatccatca agaaaactcg
    16321 agaagaacta gcagataaaa tcgagaacaa gacagctgca gtgttgtggg tgaccaacaa
    16381 ctcagccttt atggtcagca aaaatcggtc aagtggaaga atcgtatacg acgatctgga
    16441 atttggtgtt cctgatctag tggaagaggt ctccaaaaaa gccacttctg actggtctgc
    16501 tgtttcctct gaaaagctag ctgaattaga tgctgactac atcatccttg tgaacagtga
    16561 caaagatgct gccatgttca atgaaccaag ctggaagaat ctaaaagcag tgacagacaa
    16621 tcatgtactg caattcggac ctgaatctag ttggctatat aacggaccta ttgccagtca
    16681 ggaaatggtc aatgatatca aagaaaaact aaattgattt taactaaaaa aagcatcttc
    16741 acgattagtc gtgagatgct ttttttctta ttaacaaact agacgcatag attttattct
    16801 tcttttgaag caaccctatt ttttactctt tcccattttt gaaaacgatg ggcatataga
    16861 ttttcttgat ccgttatccc ctcatactcc tctataaggg cgaagtctga aaaatcaata
    16921 ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca gatccgacca
    16981 gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg aaacatagat atcttctgag
    17041 atcgtttgac tatacgacaa aagttcatcg atcgaatgca ttacttccgc attttctttc
    17101 gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc gataaggaag tgaaagatcc
    17161 cccattcctt cataggtctt tcttcccatc actaaaattc gatccattgt atgttcgcga
    17221 aagaaacgca tatcatttgg caatcgccat ggtaacaaac catttttgcc gatcagacca
    17281 tttttatctt gtgcccacat ggaaataaac atattatccg ctccttatga ttttagatgt
    17341 tttgagataa aaaaagtctc aattctttga ccagcaagga attgagaact ttaaaaacat
    17401 tattttgttt cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt ttgtttttcc
    17461 aaacgatcag gattgttacg tttgtttttg cttgttagat agttacgttc tttacaagaa
    17521 gtacactcta aagtgatgtt tacgcgcatg tttttccctc ccatttttac aagaatcctc
    17581 taagatgact ctcagacttg attatcttac catgattttt tctagattgc tagtgttttt
    17641 cacgattttg ttaagtaaaa ctacttgacg ataaatacaa cggttttact tctcaaacaa
    17701 aatctttttc tttttgtata aataccctta cacttgttta tatagattat gttttttaat
    17761 aaagggggat actaatatgc catggaacat gaaggacttt cctgcatcaa tgaaaaactt
    17821 agataaattg acaagaaaaa aagcaatcga catcgcaaat gcattattag atgaagggta
    17881 ccctgataac cgtgcgatcc caattgccat tgatcaagca aaaaaatggt atgaagatgc
    17941 tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga cggatgaaca
    18001 cgatacgaat cctcgtgctg gaaaactgct tgattcagat gtgatcgtag aatatgagga
    18061 agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt ttgatacaaa
    18121 aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa ctttgttcat
    18181 ttataaaaaa gatgggacaa aagaaaaaga aatcgattat tgaacatatg taaaggggtt
    18241 gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca atggtgttcc tctgccaact
    18301 ccgaaccata agtcataata tctataaaac gggaagagct gttttttgaa attgtttctt
    18361 atcactcagt gttaaacggt aggagaaaac ctctttttta ttgatatttg attttcatca
    18421 aacagtcaca tagtcgattg cttctgcttg cggaatcgtt tcatctacaa agtagacttt
    18481 ataccatgtc aagaaactga agattgtcca gatttttctt tgctcttctt tacgtccttc
    18541 gtgatggtca tctagcaact tcatgatttt ttcttgatca aaaaattctt ttgcaaaatc
    18601 tgcttcgaat aatgctttga cttgttcgta gccgtgttct tctttcaacc aagctttgat
    18661 tggcacaggg aatcctaatt tcacacgatt cgaccattct tccggaagat gtttgttcgc
    18721 agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac ttggtatttg
    18781 ttgagccaat ttcatgactt cgatatccaa taaaggtact cggacttcca aagaacttgc
    18841 catagaaagt ttatctgcct ttaacaagat atcttttggc atccattgat gaagatcgac
    18901 atattgcatc ttattgactt catctttgat tccttctgtt ttctcgtaat gaacagtcat
    18961 gatttcatcc acagatggtg ctgtttgata ttttggtgtt aacacttctg atgcttctga
    19021 ttcttcgaac actcgagcat gaccgataaa atattctttt gcaggagcaa gtgattcata
    19081 taaatggatc cgtccatgga agtttttcat ttttccgatt ttacgtccta aattatagcg
    19141 tgttcctttt ggtaattttt tcaatccttg agcaatcaca cggatgaatt ttgaattcgt
    19201 atggaatcca tacgtttggt atcccgcaaa cagttcatcc gccccttctc cagattggac
    19261 tacccgcaca ctttgtgaag caagatttgc taaaaagtac aaaggtacgc aagaaggatt
    19321 tgaatcaggt tcatctaagt gatactggat caaagggaat gctttgaacg acatgtctcc
    19381 gtcgatcact gctgctgtat tatctaagtc caatagttcg gttagttttc tggcttcaat
    19441 cgcttcatta taggttttat catcaaaccc gattgagaaa gaatgatctg gtttcaatac
    19501 agaggttaca taactggaat caaccccgct ggataagaaa gaaccaactt ctacatcact
    19561 gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg attttttcga tccattcttg
    19621 tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa tgtctatttt
    19681 tccatcttta tacgtataat aatggccttc aggcaaacga tagacatctt tgaagaatgt
    19741 ttcgccgttt aatggagaat attggaaagt catataaggt ttcaacgctt ctttgttcaa
    19801 ttctttgtta aaatcaggat gaggcagaaa acttttgatt tctgaaccaa acataaatgt
    19861 tccgttcatt tctgcataat aatatggttt gatcccaaag tggtctcttg caccgaataa
    19921 ctcattcttt tcattatccc aaatagcaaa agcaaacatt ccgcggatct tttgcaataa
    19981 ttctgttccc cattcttcgt aaccatgcaa aaggacttct gtatctgcat gtgtttgaaa
    20041 aacgtggcca gctgcaatca attcttctct caaaggctgg taattataga tttcgccatt
    20101 aaaaataatg atctttgtcc gatcttcatt gtaaataggc tgagtaccgc cctctaagtc
    20161 aataatactt aatcttctga atcctaaagc aacatgttta tcgatatatt ctcctgaact
    20221 attaggtcca cggtgaacga ttcgatccat cattgtatta ataattgttt ttttgttgtc
    20281 cttgtcgtta acaaaaccta caataccgca catttaactt tcacatccta atataaaatt
    20341 actgtttatt tcattcatat gcatcttatc atagaataaa tagcggacga atattttctc
    20401 tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac agaaaataaa
    20461 gtcccaatat ctaatttacc atgttttctt ttttgaggct accttattct cttcgtgtta
    20521 aaaaaatatt agtggatgct atttccaaaa aaaccagcaa aaaaagacac gacctaaaga
    20581 tcgtgt
//
LOCUS       DACGLH010000043        20444 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.046, whole genome shotgun sequence.
ACCESSION   DACGLH010000043 DACGLH010000000
VERSION     DACGLH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.046"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(20..325)
                     /locus_tag="IXT21_001776"
     CDS             complement(20..325)
                     /locus_tag="IXT21_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ4869165.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(350..781)
                     /gene="ruvX"
                     /locus_tag="IXT21_001777"
     CDS             complement(350..781)
                     /gene="ruvX"
                     /locus_tag="IXT21_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ4869166.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(778..1044)
                     /locus_tag="IXT21_001778"
     CDS             complement(778..1044)
                     /locus_tag="IXT21_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ4869167.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1299..2024)
                     /locus_tag="IXT21_001779"
     CDS             complement(1299..2024)
                     /locus_tag="IXT21_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ4869168.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2385..2455)
                     /locus_tag="IXT21_001780"
     tRNA            complement(2385..2455)
                     /locus_tag="IXT21_001780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2421..2423),aa:Gly,seq:ccc)
     gene            2572..3324
                     /locus_tag="IXT21_001781"
     CDS             2572..3324
                     /locus_tag="IXT21_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ4869169.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3340..3819
                     /locus_tag="IXT21_001782"
     CDS             3340..3819
                     /locus_tag="IXT21_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ4869170.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4070..5650)
                     /locus_tag="IXT21_001783"
     CDS             complement(4070..5650)
                     /locus_tag="IXT21_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ4869171.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5643..6386)
                     /locus_tag="IXT21_001784"
     CDS             complement(5643..6386)
                     /locus_tag="IXT21_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869172.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6562..7338)
                     /locus_tag="IXT21_001785"
     CDS             complement(6562..7338)
                     /locus_tag="IXT21_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4869173.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7586..8422)
                     /locus_tag="IXT21_001786"
     CDS             complement(7586..8422)
                     /locus_tag="IXT21_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ4869174.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8433..9362)
                     /locus_tag="IXT21_001787"
     CDS             complement(8433..9362)
                     /locus_tag="IXT21_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ4869175.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9554..9907
                     /locus_tag="IXT21_001788"
     CDS             9554..9907
                     /locus_tag="IXT21_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ4869176.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9981..10637)
                     /locus_tag="IXT21_001789"
     CDS             complement(9981..10637)
                     /locus_tag="IXT21_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ4869177.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10863..12797
                     /locus_tag="IXT21_001790"
     CDS             10863..12797
                     /locus_tag="IXT21_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4869178.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12883..14106)
                     /locus_tag="IXT21_001791"
     CDS             complement(12883..14106)
                     /locus_tag="IXT21_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ4869179.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14100..15998)
                     /locus_tag="IXT21_001792"
     CDS             complement(14100..15998)
                     /locus_tag="IXT21_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ4869180.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(15992..17110)
                     /locus_tag="IXT21_001793"
     CDS             complement(15992..17110)
                     /locus_tag="IXT21_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ4869181.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17193..18026)
                     /locus_tag="IXT21_001794"
     CDS             complement(17193..18026)
                     /locus_tag="IXT21_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4869182.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18019..18936)
                     /locus_tag="IXT21_001795"
     CDS             complement(18019..18936)
                     /locus_tag="IXT21_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4869183.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19163..20287)
                     /locus_tag="IXT21_001796"
     CDS             complement(19163..20287)
                     /locus_tag="IXT21_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4869184.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         6312 a         4058 c         3398 g         6676 t
ORIGIN      
        1 tttctcctag ctttatagtc tattcttctt cttccgccat gaatgtattg aatacttctt
       61 caatcatgtc ccattctgcg tctgtttcga tttgttctaa ctcgccttct gttccatctt
      121 cgttttcgat gtatgcataa gcttgtaatt ctacatcttc atcttctgaa acaccagcag
      181 gatataaaag aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt agtaggattt
      241 catataatgt ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt ccttcatggt
      301 cgtgggaatg gttatgttct gtcataagat tacctctttt cttttattat taaaataatt
      361 gcttacctga gccgtctaaa taattttgta agatcatcac agctgctaat ttatcgatga
      421 ctttttttcg tttggctctt gaagtatttg cctgctctac tagcatacgt tctgcctgaa
      481 cagtggttaa tcgttcatct tgataatcaa ctggcaagga gaatagttct ttgatctttt
      541 ctccgtatga catggaagct tctgcccttg gtccgatgga attgttcata tttttaggta
      601 atccaactac aaaccggtct acttcatatt gtgctactaa ttccttcaac cgatcaaagc
      661 caaactcttc ttctgcttca ttgatccgaa tgatctctac tccttgagct gtccagccaa
      721 aggggtcact gatagcaaca cctactgtct tagaaccgac atccaagccc attactctca
      781 taaattcact ccatgatttg agaggtagtc cttgacaatt acttccatga tctcatcgcg
      841 ttcgtgacgg cgaatcaaat ttcgagcatc acgataacga ggaatgtagg ccgggtctcc
      901 agaaagcaag tagccgacaa tctgattgat tggattgtag cctttttctt ccaaagcccg
      961 ataaacaatc gctaatgttt cgctcacttc ttttttgcga ctatcgtcaa aaccaaaacg
     1021 tacggtttca tctgtaaaac ccatgattct acacctcttt cccgcaaggt aattccagct
     1081 taccttctac aaccttattt tactcgaaga aagggaaaac tacaaacaaa tattccaaac
     1141 ttccgaataa attaagaaac actatttgta tgtttttttc cttttttctg tagctttatc
     1201 aaaagtactc ttttcataaa gtatacgcta tatttctttg aatgtatgtt tttacacaat
     1261 tttttcataa tattcataaa aaattcgaat agctgctact aattttttgt ttttgttgct
     1321 tgtcctgtct cataattgat cgtgtagcca tcttgaacat tgtagataaa gacattgaat
     1381 tcaatttttt tgtcttcaat gctttctgct tccaattgca ctcccctggc taccagttca
     1441 ttcccctcaa aatgaggagt gacacggtag cgtacatgat gatttgtttc tttgatatat
     1501 gccgcaatat cattttcgtg agcaagcatg tatggagcat tcaacgaacg tgtccctgtc
     1561 atcaagtttt taatgttgtt attttgacca gtcagctgga aaccaattaa atggctgcga
     1621 ttatataacc attgcccgtc acttaatttc ttttgtttcc aacctgtcgg gtcaatatac
     1681 aaaggctctc tcttttcagt agggaacaaa tcttttccaa gcatagcatt ggcttgtcct
     1741 actctgttca atttatcaat atttgaatag ctttcccatg ttcctttgga aaggctaaga
     1801 tcctcttttg taaattccgg ctgattccca ttgatttcta tttcttgatg ttttccatca
     1861 tattctggca catccgctac ttgagaagct tgtggtgtgg aatcaaaaat agttgtaatc
     1921 tgctgaaaga tatctgaatc tttttgtgtg gtggaaacac cgaatgcccc tgcaatcaca
     1981 gcagcgacga tccccacgat cgccttggtt gtttttttgc tcatttcttt actcctttac
     2041 taacagctcc ctaaatctta acatatgttc ccctataaat aaatactaga aagaaataga
     2101 aacagcaaaa aataaaacct gttttaagat ggcttaaaaa tacaaaaaag agacggattc
     2161 ctcgtctcta atcagtcacc actctatctg taaggtattt attattattc aaaaattttc
     2221 ttcatactca agacaataca ctgatttcac aaccgcttgg taaacggtgc gcaaaagctt
     2281 gcatctgcgg taataagtcc atccaagtta aaaacataag aagttgtttt tgcttggctg
     2341 ttcttattac ttgttgcaga gcagcttttt cacaacctat tttaagcggg tgacgggaat
     2401 cgaacccgcg acatcagctt gggaagctgg gattttacca ctaaactaca cccgcaatac
     2461 aaaaacagta tacgcctctt ttattccttc ctcaagtttt tttattcttt acgcttctgg
     2521 tatgttttgt agtaaaataa aactaatcaa tcaattattg gagaagatgc tatgacggga
     2581 aaagaattag atctgttgct ggacacttgt ttactcgctg ggaaaatcat gatggaaagc
     2641 aatgcggaga tgtatcgggt agaagatacg atgagtcgaa ttgctttagc ttctggaaat
     2701 ttccgtttgg tcagctatgt cactcaaacc ggtctcttta ttggtcttga tcgtacctcg
     2761 accatccgaa tggaacaaat taccaaccgt tcgattaatt tagaaaaagt agtaaaagta
     2821 aatgatcttt cacgtaaata tgttgcttca gagctaacat tagaagaact ttactgcgaa
     2881 ctgcaagcaa tcgaccgaga ccgttctttc tttcctgtct ggttgcaaat cgttagcgcg
     2941 gcagtgatca gtggaacaat tatggtgtta tttggcggaa agatgaccga tttacctgtg
     3001 actttagtca ttggaggatt gggatacgca gcttatctat atagtttgaa attttttaga
     3061 atcaaatttt tatcggaatt tttatcttct ttattgattg gcatagccgc tatctacagc
     3121 gtacgttttg gcttaggaag caatcaggat ctaatcatta tcggatgtgt gatgccttta
     3181 gttcctggag ttcaaattac gaacgctatc cgtgatttgc tagctggaca ttatgtctca
     3241 ggagtttctc gcggaacaga agcgatgatg actgctacga tgatcggttt tgcgattgct
     3301 tttattttcc agctttttta ttaggaaagg aacacaagta tggggacgat atttattcag
     3361 ttttctttta gttttttagc gactgcggca tttgccgtta ttacaaatgt acctaggcgt
     3421 tcattgattt gttgtggtct ttctggtgct tttggctgga tgatctactg gatctgtgtt
     3481 caaataggcg gatctgctgc ttttggttca ttgcttggtt ctttaggagt agcattcatc
     3541 agcgatcttt tctctaaacg tttgaaaatg cccgtaacga ttttcaatat tcccgggatg
     3601 gttcctctag tccctggtgg tttagcttat caagcggttc gaaatctcgt tactgaatcc
     3661 tatcgagaag cggctcatta tgcggtccaa gcaattatga ttgctggagc aatcgcttta
     3721 ggtcttgtat tatcagaagt ctttaaccat aacatccgta atttcaaagt aaagcgtgac
     3781 gcaatcatga tgaaacgaaa aaaacggctt tttaaataaa cgaagtgtaa caaagaaaag
     3841 gttgtgaaag agacgttcag ccttgagcga tgacaaaata tctcacaaac attgcgacaa
     3901 gtaaccgcag gagcaataag aagtaattat gagtattaaa aaaagttttg taaaaatagc
     3961 ttctcacatt tttacataaa attgacttaa tactgagaaa ctgacttttg aacaccctct
     4021 atctgtaaaa aaggggctgc gacatgattt ttgtcacggc ccctcttctt tattcaaatt
     4081 ttttccaaaa cgttcgttga taataaacga tccatagagc ggaaagtatt agaactaaac
     4141 cgatcacagg aatattcact aaccaaaatg gataaaacaa agccaacatt ccgtataaga
     4201 cgataagaat aatactgatg atcaatgaac ccatcatagt tgcgactaaa cccaaacttc
     4261 ctgaacctct gttaaataat tggttaatat tggtccagtt cagcagcagc aaccgataat
     4321 ctctagcgaa ataacgtagt gacaataagt agcagccgaa taaagaacca aatagcaagc
     4381 tagggataaa aaacaaaggc agctgaaaag acagtccgct taataatgcg atcccaccag
     4441 ttaaaaaact ctgcaatatc catccgaatc tgaacttttc ttttaaatat tggttcatag
     4501 agattggcaa agaacggata aacagaaaat tttcttgatc caaagaaata agattgctga
     4561 taaaagaagt ctgattaact gttaagaaag ccaatgcgat ccctaccagg aaaacaacac
     4621 cgattaactt attatctaaa tcagttaatt ttatttcgcc tcctagggca aaagcaagaa
     4681 taaaaatgat tggagttaac aaggaagaag aaagaacttg cataatcaaa ttaggttctt
     4741 ttactaattg agcattgtaa ctaaataaaa gctgatgcac attttggttc tttttatggg
     4801 ttctttttat tttgaactca ctgtttgaat cagtcagttg ctctgtaagc tttggtacta
     4861 tcagctgttt tacagcaagg aaaaatccca gagccgtgat agctagtatt ccccaaccca
     4921 ataaaccttg ttgagaaagt ggctgactgg cagcatcata gaatggcaat aatatcgaaa
     4981 tcggatgccg atctgttaac tcattttcaa tagtccccgt ttgatgattc atccacatga
     5041 ttccagcaaa tgcgatcagc atcgagctgc caagcaagat actagaaact gtttttctat
     5101 gttttttaaa cactgctgtt cgagccaaac caaaaacaat caaactagcc agacaaaaaa
     5161 gaataatcaa aaatagagaa aacaaaagga ttgagataat gatagtaagc aaaatcccta
     5221 gatcagctcg ccatccggtt aaaataaaca tgactagaag cggaaaaaca aaaggaatga
     5281 tggtcaaagc aactaccaaa atctttgctg aaaaaacctg ttcttgtcca aaaggcagag
     5341 gcaaaaatac ttgcaggtca tttccttcaa aaaagatatt ataaatgact gatatacttt
     5401 gagttaaacc taacatacca aaaagtgcca cgtaataagt aaagaaacct ggcaatttaa
     5461 caaagttcat agcaaacata gcaaatccgt aaatcagtaa aaaaatcagc cctgaaaaaa
     5521 gattttgaga caaaagatag atagttaatc gctttccctt ttttcctttc tctcttgctt
     5581 tattcgtgag ctgtggatta acatataaaa gattaactcg aattaattct aataattgtc
     5641 gcttatacat acggtccatc tcctgtcgct tgccttccag ccattttcaa ataaatcttt
     5701 tctagcgttt catcaggtga gccattcaat aattcactaa ctgccccctg ataaatcaat
     5761 tcacctttct tcaaaatagc cagtttatcg cataactgtt gtgcggtatc caatacatgt
     5821 gtagaaaaaa tgactgtttt tccttttgcg gcatgagcct tcatcatttc tttcaaatca
     5881 aatgctgctt gaggatcaag tccttgcaaa ggttcatcca atacccaaat atctggatct
     5941 ggcagcaacg tgccgatcaa aatcgttttt tgacgcattc catgtgaaaa acttgcgatt
     6001 gtttcatttt gatgactata catatcaaaa agcgctgtca attctgctaa acgcttttct
     6061 tttttttgtg tgtctaattc ataggcagct gaaatcaagt cccaatattc tccagccgtc
     6121 aattgtaaaa aaatatctgg ggaatcaggc acataaccga ttttttgttt gatactcaat
     6181 cgattttccg tcaatttcat tccatctact aaaatcgttc ccgatgatgg ttcaataata
     6241 ctaactaaac ttttaatcgt agttgatttt ccggcaccat tatgtcctaa aaatccaaaa
     6301 atttcccctt gtcttatcga gagagacaag tcttttaatg cttgtttttt cccgtaactt
     6361 ttacttacat tgatgaattc aatcatttct gtactccttt tgtttgttat tactttttat
     6421 tgtacccttc gttattggtg gagtaaatgt caaaaagagt gcataacaaa aaaggataga
     6481 aacgactagg ctgctacttg ggttgttctc ttctacagga aaacactctt ctaacaggta
     6541 agatatgagt ctttgttttc tttaaaatga ttcattttct atcttttctt gcataaaatt
     6601 ctttagttta tcaatacttc tctttcccat ctctagttgt tctccatttg caaatgttac
     6661 tatttcgttt actctgctaa tagatgtaat gttttcagta ttaatgatat aagcttttaa
     6721 atctttacat aaatactcat attcgagtga ttttttatat tttttaattg tgccgtttac
     6781 gagaatctct tcatctttca atactaggcc tattttattt ttataacctg gcatgctcat
     6841 tagataaagg atttcatcgt aatggatata gtgatcgcta ttaccataac ggacagttgt
     6901 ccaccttttt ttcgtagcat gttgaacagg atagttttct ttttctttca ataagtaact
     6961 ttggatgctt tttttatttt tttcataagt gaattctttt atcaaataac cactaggcgt
     7021 aatctttcga ttaataacag ttattgcttt attcggatga gacgttagat aaacaatttt
     7081 acacttttca ttcttttctc taagcatttc gcctagttcg ataccgctat aataggcgtt
     7141 caaatcgatg tctaggaaaa aaatatctga ggaatagatt tcattatttt taatgaattc
     7201 aacaaattgt ataacgttca atccctgaac gatctcaaat ttccaaggac ttaataaaat
     7261 tctggattct tccaaaattt ctactagcat tttttcaaaa accaaactat catccaaaat
     7321 aaatatacgc gttaccataa aaagtacctc cactcgatat tttactatca aaatcatttc
     7381 atacaacttt tcaacataca tgttaggaat aaattgtaat tctcttcaca atcttttcca
     7441 acacctttga aaccgatatc aaatactttc aggatgctgc ttctaaaata aatatcaaac
     7501 aaatgaatac atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg
     7561 agctatgttt atttattttg ttcttttaaa aagaacgata cacatactga tttttcggag
     7621 cagagacttt ctcagcgttt gtaacaacta cagatgggat ttcacgcttg aatggttgat
     7681 aatagtctga ttggatggtt ccttctattt tataccattc atcattttta accgttactc
     7741 cttccggcat atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa tggataatgc
     7801 cgaatcggaa caagaagata tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt
     7861 catagctgat ttttttgcca atgaattcgc tagggtaatt ataaattagc tccataactt
     7921 ctaaatagtt ttcatctgtg atcttgatag tttgcttatc cccatacttt tctttcaact
     7981 cattcatttg gtctaagtag tctgatttgt tgaaataaat actggtatcc ggctttaagt
     8041 attgtgtctg catctccgga tcccctactg attctttact tactgggaaa ttaaatcctt
     8101 tggcttctac gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc acgattactg
     8161 gcaaagctag cagtacataa gcgattccac gttgataagg ttttgacaac ccatggtcat
     8221 gatcgtgctc gtgtgccttt tcttctgctt ttccgttttt gacccacaaa tacagctgaa
     8281 ctaaagccaa aagagcagat aaaatcattg aaagaatagc taagtagcga taatggacat
     8341 tgatatattg atcgagtttg ccagaaactt gcaagtacat catcaattcg aaatacccag
     8401 ataaaatcaa aaaccgcagc atattttccc tcctaaataa ataatgtgaa tccaataacg
     8461 ctaagggcaa tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc
     8521 atcagtagat ttttgatatc gaccattggg ccaaagacca aaaaggcagc tacaggtgct
     8581 gtgccgaaca gacttaacaa agaagatcct ataaacgcat ctgcttctga gcaaagagac
     8641 atcgtaaagg ccaataacag catgatgata atggctaaaa tctttgttga gccgagcgtt
     8701 agcataattc tagttggtaa ataagtctgc atcgcagctg cgattaatcc gccaatgatc
     8761 aaataacgac ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg cccagtttga
     8821 tggatcagct gattttttgg ttcattcttc tcactttgtt cttcttcatg gacatgatga
     8881 tggtgatttt ctgctacttt agacgtgatt ttttctttca atacagcgtc tttttgaaaa
     8941 taggcaagcc agacaccaat cacaagggca acaaaaaagc tgcctatcac ccgccagctt
     9001 gccatctgcc aagaattgcc aaatgcgata aaagtggaaa aaatcacgat tggattgatg
     9061 atcggagcag tcagcataaa agcaaacgct gtgtgaactg gtacgccttt tttaacaaat
     9121 tgatgaacga tcggtacgat gccgcattca catgaaggaa agaaaaatcc taagatcgaa
     9181 ccagtaaaaa tagagaagat acgattttca ggaagccatc tcttcacacg ttctggggtc
     9241 aaaaatactt gtaaggcgcc tgagataaga cagcctaata atacgaaagg taatgcttca
     9301 ataacgatcg ataagaaaat cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac
     9361 atgagtttcc tcctttttat ttctagttca ttcgctttgc aaatatacgc ttttattcaa
     9421 aaaaaacaag ttgctctgta aatttttaca caactcttat tttttaccaa agcacttcaa
     9481 ttgacttttt ctgttattcc cattaaatta aggtctcata catatcactt ctatcataaa
     9541 agggagaata aaaatgaaac aaaccactaa cttgactgaa gttcaagaca tactgcaaca
     9601 gtcatccgtt gccgtcattt atctttcgat gcctaattgc agtgtttgtc acgctgttcg
     9661 tccacgacta gaggaacttc tcacacatta tgatattccg gcacttcatt tggatgcatt
     9721 tgaaacacca gaagtagcta gtaaatttga agtgctgact gcgccagttg tcctgatttt
     9781 tcatcaagga aaagaagtct ttcggcaagc acggttcatc gactttaaaa aaatccgtta
     9841 tctactagat gaactgacag cagaagatga ttccctttct tatgaagaaa tcttccgaac
     9901 aaaataatcg ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt
     9961 tgttcaatcg ctttttattt ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa
    10021 tgcaagaaat agatcaaggt ttgcaattca ttcgtcaaat caacattttg gatcaataca
    10081 tctgacggag cattcaatcg agcagcggta aagttcaaga ttcctttgat tcctgcttcc
    10141 gttaactgat tgactacttc ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg
    10201 atttgttgga tcttgatttg ttctgacata tcttccattg gataaacggg gataccgtct
    10261 acgatgcgcc caacgatttc ctcattcaca tcaaaagcac aactgatacg tatgctatta
    10321 ctttgatgga atttgtattt tagtaaagca cttcccaagt tccctacacc gatcagcgca
    10381 acatttgtca attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag atcttctaca
    10441 tcataaccat acccacgttt tcctaattcg ccaaaataag aaaaatctcg acggatcgtt
    10501 gcactatcta cttgaacggc ttcacttaat tcagttgaag acactttttt ctttccagca
    10561 ttatttaaaa tccttaaata gcgataatac aaaggcagtc ttttagctgt cgcctttgga
    10621 ataatatgat ctttcacgaa atgacctcca cgtattgtct aattacatct ttgatttctc
    10681 catcatagca gtatttatag gaaaaaatca attccattgc tcagatattg tgaaatttta
    10741 ttctttattc atttatttat catttctgca aatctcgcag tataatggaa tttatggtag
    10801 actactaatt gaaaaatagt tttttatttg aggagaattc acaaagtaga aaagaggatt
    10861 ccatgatatt attacaagcc aatcagattg ctcgccattt tggtgcagaa gtcttatttg
    10921 aaaacataca tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg
    10981 ctggaaaatc gactttatta aagatcattg ctggaattga agcacctgat gaaggaaaga
    11041 tcgctaaaaa taaaacagcg actctcggat acctcgctca ggacacagga cttgattcca
    11101 atgaaacagt ttggaacgag atgctgaaag cttttgaaca agttagacaa atggaagagc
    11161 ggatgcgcga gctagagatt ttcattagcg aaggtacgcc agacacacct gcttatagtt
    11221 cttatttaaa agaatacgac cggctgcaac acgatttttc agatcgaaac ggctatggtt
    11281 atgaaaatga aattcgttcc gtattgcatg ggtttaaatt cgattcttca ttttatgatc
    11341 agaaaatcga tactttatca ggcggacagc gcacacgttt agctttagct cgcatgcttt
    11401 tgcaacaccc tgacattttg attctagatg aaccaacgaa ccatttagat atcgagacac
    11461 tttcctggct tgagaattat ctgcaaagct atactggtgc attattgatt gtttcccatg
    11521 accgttattt tttagataaa gtagtcaatg aagtctatga attaagcaga agaaaaatgc
    11581 gccattacaa aggtaactat tcgaaatatc tagatttgaa agcagaacag ttagcaagtg
    11641 aatggaaggc ttatgaaaag cagcaaacag aaatccataa attagaagac ttcgttgctc
    11701 ggaaccttgt tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag acattagaaa
    11761 aaatggaccg gctggatcgt cctcaagggg atgaaaaaca agcaaacttt ctattcagta
    11821 tagaaaagcc ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc ggatatgata
    11881 atgtcacctt atctgaacca atcaatctag atatccgtcg tcaagaagcg attgctctag
    11941 taggtccgaa tggtatcggg aaatcgacgt tgctgaaatc aatcattcac cagatccctt
    12001 ttatccgtgg aaaagaaacg ctagggacaa atgtcaccat cggctattac gatcaaggtc
    12061 aagctgatct gcatacaaac aagacgatcc tagcagaatt gtgggatgag catcctacca
    12121 ctccagaaaa agagatccga agtgctttag gtgctttctt attttctggc gaagatgtag
    12181 aaaaaccaat ccctctgcta agtggcggtg aaaaagcacg ggttgcttta gccaagttag
    12241 cgatgaacaa agaaaatttt ttgattttgg atgaaccaac aaaccattta gatattgaca
    12301 acaaagaagt attagaaaat gcattgatcg attatgacgg cacactgctt tttgtttccc
    12361 atgaccgata tttcatcaat cggatcgcga caaaagtcgt cgaactctct gaaaatggca
    12421 gcaaagtcta tctaggagac tatgattatt atttagagaa aaagcaggaa gaagaagaaa
    12481 tcgccgaatt gttggcacaa gatgagccag aatctgctcc tgctcccacg aacaaatctg
    12541 attattacca aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga aaaattactc
    12601 aggttgaaga agaaatggcg cggatcgatg aatggatcga tcagttggaa caagaaatgg
    12661 tcgcgccaga aaacctggaa gatcacgtaa aactcaatga gctgaaccaa gatttagaag
    12721 ctgctagaca agatcaagaa acaaaattga cggaatggga agaactaagc ttggaattag
    12781 aagaatatga aaattaacga aaaaaccaaa acagaaacca ttcatgctgt tttggttttt
    12841 ctttttttat tttatcaaac aaaaattcca tatttacctt tttcaccaat aagaagacca
    12901 attactagtc attctcacta aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt
    12961 atttacccca ttatttttgg gcttattata aacagactgt gttaaaaagc catcttcttc
    13021 ttccgttgta gcatatcttt ctttcaattg tcgcataatt ttccaaacag cagactggta
    13081 taagttcttt tctgttttgt ctaatagacc tgattctatt aattctaata atccacaaac
    13141 catgatcacc atactagatg tatctttttc ttctctgcta gaatcatcaa agatcaaatc
    13201 ccaataacaa atggtatctt ctggcaaccg attcataaaa taattggcta atattttgct
    13261 taaatcaaaa tatttttgtt ctttcaaata acgataactt aatgcaaaac cgtaaatccc
    13321 ccatgcttgg cctctagccc aacaggaatt atcactataa ccttgatgcg tacttccata
    13381 tcttggtttt ccatctttga catccatata aaatgtatga taagttgaac catcttctct
    13441 tactaaataa tctgctgcat tttgaatatg agcctgagca gcttttttat atttttgatt
    13501 tccagtttct tcactcgccc aatacaataa tggaagattc atgcaacaat ccatgatcat
    13561 tctaccttgt tgtgaaggat cactaggatc tccccaagct tgtataactc ctgccttctc
    13621 ccaaaaacgc tgaagtaatg tttctgcagc atcaattgaa gcttccttca tttttcgatt
    13681 tcccgttatt ttataatctg ctacggttga taaagaatac aaaaaaccaa gatcgtgatg
    13741 attagtgata atttgacagt caatcctgtg gataaaatct atatctgctt tttcagctgt
    13801 ttgttgaaat aaaggatcac ccgtcttctc aaataacaac caattaattc ctgtccaaaa
    13861 tccttctgtc caaccaatat tgtctcttaa tggataacgt ccttttatag gacactcgct
    13921 tgggaattga tactgactgt tactgatacg tttgattaac ctctccgtgt gtctgcgaca
    13981 acgctcgatc tcattattcc attctttttc cattaattct ttataacatt catttatcca
    14041 tttttctgga tgtattatct cttcaaacat cactttatca agcatttccc caccagcctt
    14101 tagcataatt ttagtctttt tttattgttt ttattttgga tagttagccc ggttgtccca
    14161 tcaaaatcaa ttttgggacg attgttccat acttctaaac tataatcccg atcttgatga
    14221 gcaaccactc ctgttccgag ccaatgggtt ccttttccaa atgtaccctc taatccagga
    14281 ataaaagtgt tttcccatgt catcaagttt gtattgggca aacccataat agctgtggat
    14341 tttcgtttaa tagtatcgtc acttaaacag ataatacctg aaaagccagc ttcactcacc
    14401 ctatagtttt ccttgtcttc ttctatgatt attccttctt tctcaggaga acattcaata
    14461 gcaaaccctg tctcatatag aacatattct gtatctaatt ctactttgtg tacgcgaatc
    14521 tgccagcctt caaaaggaat aagccaagtt ctgatttttg cctgtaaagt aggttcccat
    14581 gtagaggcaa gtacctcttt cttacataca atgtctcgta cattatttcg aacgatgaac
    14641 tgtccatagg ttttgatgct ctcattctta aaactggtcg tgatatatgt gtccgctcgc
    14701 tgaatcccta gcattgaatc cattgccatt tctgctttca attgattcgc tcgcggtatc
    14761 gaaaaaccaa attgactaga ataagcaaat tttaaatatt tttcattggt caatgtatgg
    14821 aaatttggcc ctaattgtcc cgcattcaaa aaaaatgtat gtccattatc atgttgtatg
    14881 agcaaattgg cttctgaaag aagtttagtt ccttcacgaa taggcatagg catttcttta
    14941 gccgtccaat actcatggtc ttcaggtagc gccaacaata aaaaaatctt attcagccat
    15001 aatggagata acgtactatt atagggttca gtcaatgtca attgagggta ggtataacca
    15061 atattcagga tacctttttc atcaaatatt tctttttgca tccaccatct tatattccga
    15121 ttgactaacc ccttgatttc acctaattta aaaggcaaaa gatcggccca caacatagct
    15181 gaccaaaagc ttgaaactgc aaaacgataa atcatgcttc gaccaaaagg cacatttgca
    15241 ccatcttccg caaaaaaatg aatatagtct ctagaaaaat caatagctcg gttcttatat
    15301 atctggctaa tgggcttttc tggttctagt acgctataaa tcaaaccata ataatgaaat
    15361 gcaaaaggat tataataatc ttggcgacct cttgaactgt ctcgatacca gccattttga
    15421 agataacaat tttctatatt atcaaatgct tctttcagtg ctgattcttt aactggataa
    15481 ccaaattttt ggataattgt tccgacaatt atcttaaaaa attgccaatt accatctgca
    15541 cattcatatt caaaaatttg cgagaaccaa tgaataatct ggcttttttc tcctgtatct
    15601 aattttttcc ataataattg ttcatgatga tataacgcta ataacattgc gggcatttct
    15661 accatacgct ggtctctttc atgtatttct ccccaataat cgggatggga aggatctgtt
    15721 ccagctttta ttcctgatag aattaatttc aaccatcgtt catctaattg gtcaattgcg
    15781 ggccccgctc cccataaaac ccgagagaat ccttccattc ctgcaatttt atttccatat
    15841 cctacccctt gatgtatata atcaatttgt gcgccacttg ctcgataaaa cggctggagt
    15901 ggttgattta tctttttaaa taagtcaaag taatcctgtc tggtccttaa tagtggtact
    15961 ggatgtttca ttgtattttc tcctcttttt actaccaaat agctaattcc gactgttcta
    16021 atcgaagtaa gccttcaata aaaaaataat cagaatagat cagtggcaca ttataagtca
    16081 gttctctatg gtatgcttct gctccgcctc caatgatccc atcttcatcc atattccaat
    16141 ttgcatagtg cttttctgtc ttttggagta tattgatagc agcttcatga tacatttttg
    16201 atcgtacaga ggaaacatat cctgctaatt ccagaagacc gcatgcagcg catactccag
    16261 cactggtatc aatattcgaa tcttttacag caaaatcaat gagaggaata taaccagttt
    16321 tttcgatttt ctgaataaaa tttactgcta tcttttctgc tatagctaga tagtacggat
    16381 tttttgtgta acgataactt aacgcgaatc catataaagc ccatgcattt cctctgctcc
    16441 aacaagaatt ctcattgtat ccttgcccgc caagtaaccg aatatatttc cctgattctg
    16501 catcaaattc tgcgatatgc ccggttgatc catctttcct cactagatac ttagctgttg
    16561 tatctgcatg ctgccgagca attttagaga acctcggatc accagtttcc ctcgaagccc
    16621 aatacagtaa tggtaaattc atcatactat caataatcat ccaaccaggt ttatggtcat
    16681 tccatgctac taaatagttc cctgaaatat tatagcgtcc agcaagtacc gtagctgcat
    16741 gtaaccctag ttgtcgacta tgtttgtttt gagactgttg ataatgtgca atagccgatg
    16801 gtaagaataa aaaacctaca tcatgatgaa cgtctgaaaa atggtacagg gcagattcta
    16861 ataattgttc ttgttgtatt gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga
    16921 gctgccataa aataccactc caaaaaccat tggtccacca gtcgattcct ctgtccttta
    16981 ttacgtctgt atattttccg ttgtaagaaa tatatggaat tttattcccc attctgtcta
    17041 attccgcatc tatcttttca gaagctacat tcaaaatttt tcttgaccat tccttcgtct
    17101 gttcattcat ggatatcccg ccattctttt aatttttatg aatgtggagt tgctcctaga
    17161 acgaactaga tggttaacat tcgttatttt ttttacttat ttgcagctcc aatgtcattc
    17221 tccttaaatt tagactgcgc ccacacataa atcaagacca ttgggatcac tgaagttgct
    17281 aaaattagca taattgcgcc ataattaatt ggctcacctg tactagaaac cgtcatattt
    17341 ttgataatta agggaaccgt atatttatct ttatctgaaa gcatgactaa tggaaataga
    17401 tattgattcc aacttcccat aaagataagt aaaccactag taataatcgt aggtgataaa
    17461 atcggtacag cgattttcgt aaaaatcacc cactcattgg caccatcgat tctggcagcc
    17521 tccatcagtg ctgtaggaaa ttgactgcta tattgtcgca tcataaacac tgaagttgta
    17581 ctggcaatcg aagggatgat cacacctgca taagtattcg ataatcctaa acgactcata
    17641 atgtaaaata aaggaatcaa tgtggtaaaa aaaggcacca tcctagatag cataactgta
    17701 ttgaacaaca gtttattccc cataaaacga tactttgcta atgcataacc cgccattgta
    17761 gaactggctg tagctaaaac tgtactgatt acagcaataa caatcgaatt ccaaataatc
    17821 aagggataat tatatctcgc catgatggct tgccagtttt ttaatagcat atccccaaac
    17881 ttgaaattga ttccgccact taaaatttct tgattacttt gcgttgaagc tacaatcata
    17941 tataaatatg gcatcaaaaa tattaaggca ccaaacacaa taatgacgta cgtaaaaatt
    18001 ctgctttgta tcttttgttt agtcattttt atcacctccg aatttaaatt gaatagccga
    18061 taatataaca gatacaaaca ccatcgtcca ggcaatagca gaggcttttc caaaatcaac
    18121 catttcaaaa cttgttttat ataaattgac tcctacgacc aaagctgaat tttctggtcc
    18181 attggaagtc ccaaagaata tatttgggat attgaaatta cccaagccat tgatagtggc
    18241 taaaactgtg gccattaaaa tgattggctt tagcatgggg atcgttaata aaaagaattg
    18301 ccgaaatttc gatgctccat ctatttcagc actttcgtat atctctttag aaatcccttg
    18361 aagaccagct aaaaataaaa tagtgttata tcctacacta agccaaatca tcgtgatgat
    18421 cattgatatt cgtatccaaa aaggatttgt taaccactgt acaccgttaa ttcctagcgc
    18481 attcaaaaat ccattgaata acccagtcgg attgaaaaaa gtcatgaata cggaagctac
    18541 agccaccgtt gatgtaatgg ttggaatata atagacagtt ctaaaaaatc cttttccttt
    18601 taactgtgct gaattcaata aaacagcaaa gtaaatagct aatagcatcg ttattggaat
    18661 tgatcctgcc atgataatca aaacattcgt gtaagattta ataaacaatg gatctcggaa
    18721 gatagctaga taattgtcaa atccaacaaa ttcaaatgag tagccaattt tcataaaact
    18781 aaaagcaaac gagataatga tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg
    18841 tgtgaccaat ccataagcac tttttttatg agacgtatct gttgcttttt tcttgctgtt
    18901 ctttattgtc ttgcttggta acgaagattg catcatcgtt aagtcctcct aatatttttt
    18961 aatcaccagt taattttacg ccgtacttat gagcaaaatt ttctgcttct tttagatatg
    19021 aattttcaaa atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa taagacgtag
    19081 catctgaata agtggctggt aaaacagtat ttgatgtatt ttcattgatt ttattcaata
    19141 gttcatagta tttttgtcct ccttagtagt caaacttttt ctcagctgct tctgtttgat
    19201 aggccaatga gttagatgta gccacgccca gctccagtgc gttggctaag cattcttcat
    19261 ctgtcagcat aaatgtaaga aattgttgtg ccacttgtgg atttttggca tctttaggga
    19321 cataaaaact tgatcctcct gatactgcac cttttcctgg attttctttt gaaaaaggaa
    19381 tcagtggagc catgcgccat ttgtcagatg catcaggaaa attcaaagac atattcgtag
    19441 cataccagcc gccagcaaca aacattgcag attcttgaaa tgctttctca gcatcttgtg
    19501 caccgtagaa atgtacaata tctgagtcga tcatttcttt gattaacttt gcagcatgtt
    19561 tcgcttcttt agtattcaga cggatatttc cttcttcatc aagcaaagat tgattctgct
    19621 gggctagcat gttgataaac aattcttggt ccccagattc tggcaaggca attccaaaac
    19681 taccagtttt ttcttttaat gcttttgcca cctggatata ttcttcccaa gaactgattt
    19741 cttcaaaatc tacacctatt tcagcaaatt tttttggttg ataataacaa agtgctgggg
    19801 taaaatcact agcccaagga ataatcgttt cattactcgt ttgttgacgc aaaatattca
    19861 atttttgatc ataaaactga tcgccatatt tgtcataaaa tccatattca tcagcactat
    19921 aaaaagcatc ttgaaatttt tccagcacac ctgctgctga agtgtctgcc atggagataa
    19981 tatccggcat atcttcgtgt gttaccaatt ttggggtgat catttgtact gttgctgttc
    20041 ccgaagctac attcacaatt tttaagtcaa tatttgctcc agttttgcgg ttatagattt
    20101 ttgcagcatt ttcaacatag tttgtcagtt tgccgtttgc gacatatact tctaatgtag
    20161 tggctttttt tatcattgga tctgcgatgt ttgctgttac ttcttcgtta gtttttccac
    20221 atgcacttaa gctgaataat agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt
    20281 ttttcatgtc tatcccttct ttcttttcaa gttcttatat aaaacgcttt cacttatgta
    20341 tattaacgga attagtaaca caaatattta tataaaaaaa tattttactt gtcgttttgg
    20401 cttttcattt ttgttataat agtcattaat tataaataga aagc
//
LOCUS       DACGLH010000044        20293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.047, whole genome shotgun sequence.
ACCESSION   DACGLH010000044 DACGLH010000000
VERSION     DACGLH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.047"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            272..2743
                     /locus_tag="IXT21_001797"
     CDS             272..2743
                     /locus_tag="IXT21_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4869185.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2846..4582)
                     /locus_tag="IXT21_001798"
     CDS             complement(2846..4582)
                     /locus_tag="IXT21_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ4869186.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4579..5283)
                     /locus_tag="IXT21_001799"
     CDS             complement(4579..5283)
                     /locus_tag="IXT21_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4869187.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5414..5917
                     /locus_tag="IXT21_001800"
     CDS             5414..5917
                     /locus_tag="IXT21_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ4869188.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5877..6215
                     /locus_tag="IXT21_001801"
     CDS             5877..6215
                     /locus_tag="IXT21_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ4869189.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6236..7654
                     /gene="ffh"
                     /locus_tag="IXT21_001802"
     CDS             6236..7654
                     /gene="ffh"
                     /locus_tag="IXT21_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ4869190.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7766..8344
                     /locus_tag="IXT21_001803"
     CDS             7766..8344
                     /locus_tag="IXT21_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ4869191.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8348..8869
                     /locus_tag="IXT21_001804"
     CDS             8348..8869
                     /locus_tag="IXT21_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4869192.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8948..9502)
                     /locus_tag="IXT21_001805"
     CDS             complement(8948..9502)
                     /locus_tag="IXT21_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ4869193.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9755..10030
                     /gene="rpsP"
                     /locus_tag="IXT21_001806"
     CDS             9755..10030
                     /gene="rpsP"
                     /locus_tag="IXT21_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ4869194.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10042..10293
                     /locus_tag="IXT21_001807"
     CDS             10042..10293
                     /locus_tag="IXT21_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ4869195.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10418..11209
                     /locus_tag="IXT21_001808"
     CDS             10418..11209
                     /locus_tag="IXT21_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869196.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11379..11900
                     /gene="rimM"
                     /locus_tag="IXT21_001809"
     CDS             11379..11900
                     /gene="rimM"
                     /locus_tag="IXT21_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ4869197.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11890..12651
                     /gene="trmD"
                     /locus_tag="IXT21_001810"
     CDS             11890..12651
                     /gene="trmD"
                     /locus_tag="IXT21_001810"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ4869198.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12787..13134
                     /gene="rplS"
                     /locus_tag="IXT21_001811"
     CDS             12787..13134
                     /gene="rplS"
                     /locus_tag="IXT21_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ4869199.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13265..14401)
                     /locus_tag="IXT21_001812"
     CDS             complement(13265..14401)
                     /locus_tag="IXT21_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4869200.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTKKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYIEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAITI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14513..15468)
                     /locus_tag="IXT21_001813"
                     /pseudo
     CDS             complement(14513..15468)
                     /locus_tag="IXT21_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
     gene            complement(15653..15961)
                     /locus_tag="IXT21_001814"
     CDS             complement(15653..15961)
                     /locus_tag="IXT21_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869201.1"
                     /translation="MDLEKERIKINCNFCKKDFILTFNTTQCPNCGGKFSPEEVHQVF
                     YNYESRLANSKLYRAGEKMQKSGERIEKTGSFISQIGCFIFMLPLGLFCIWFIMNMFK
                     "
     gene            complement(16100..16753)
                     /locus_tag="IXT21_001815"
     CDS             complement(16100..16753)
                     /locus_tag="IXT21_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869202.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKEA
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(16803..17231)
                     /locus_tag="IXT21_001816"
     CDS             complement(16803..17231)
                     /locus_tag="IXT21_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ4869203.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPRKIEELKNLYGLPESAYRFLI"
     gene            complement(17236..17610)
                     /locus_tag="IXT21_001817"
     CDS             complement(17236..17610)
                     /locus_tag="IXT21_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4869204.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17895..18080
                     /locus_tag="IXT21_001818"
     CDS             17895..18080
                     /locus_tag="IXT21_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4869205.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            18092..18340
                     /locus_tag="IXT21_001819"
     CDS             18092..18340
                     /locus_tag="IXT21_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869206.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(18588..19055)
                     /locus_tag="IXT21_001820"
     CDS             complement(18588..19055)
                     /locus_tag="IXT21_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869207.1"
                     /translation="MSIISFILSTFLAGVKIKGMRPQLDVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSSKLDRRWPATPFKKLIAKGGLDDNRIYSESVPLNIPPKSAM
                     SFYLAFEVGKINFKEVLNHQTKMIFTLNRTQIHKVVDIKSTNFPVEKLVKELN"
     gene            19141..19866
                     /locus_tag="IXT21_001821"
     CDS             19141..19866
                     /locus_tag="IXT21_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ4869208.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNI
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETGIRMEIDQLNRQQRLFGDENE"
     gene            19859..20029
                     /locus_tag="IXT21_001822"
     CDS             19859..20029
                     /locus_tag="IXT21_001822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869209.1"
                     /translation="MNRAEALRIGTLIANRWWRHNKPNILSRQHIEKKKAWQQIKKVT
                     QPTKAMSHSNKS"
     gene            20048..>20293
                     /locus_tag="IXT21_001823"
     CDS             20048..>20293
                     /locus_tag="IXT21_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869210.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELECDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLTDA"
BASE COUNT         6634 a         3516 c         3957 g         6186 t
ORIGIN      
        1 gagagactcc tttggtagga tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt ttccaccaac
      121 acaaaatatt atagaaccct ttttttttgc tatatgacag gaatgaaagg tgtttgcaag
      181 cttaatgtga ggaacacttt ccatatctgt tctatactgg ataggagaag tttggaaata
      241 tccagaaaac gaaatcaatc gaggtagcca catgaagaat aaatggataa atcgaagtaa
      301 aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc cgtctttttc catcgtataa
      361 agaaggatta acggcagatc aagtcaagga acgaatagaa aaaggtgcag caaataattc
      421 agtcgatccc acattcaaaa caaatcagca aatcgtattg gaaaacatat ttacgtattt
      481 caatttgatc tttcttatct tagccatttt gctttgttta gtagaatcct ataagaactt
      541 gactttttta ccagttatca tagccaatac aggaatcgcc atttttcagg aaattcgatc
      601 caaaaagatc ttggacgaac taaatgtttt aaatacagca aaagtcaatg tcatacgaga
      661 cgggatagaa caaacaatag atatagaaga ccttgtaata gatgacattg ttattttaga
      721 gactggatat caaattccag cagatgcaga ggtcgtggaa gggaaactgc aagttaatga
      781 ggctttattg acaggcgaag cagatgaaat aaccaaagaa ataggtgatc cattaatgtc
      841 cggcagtttc attgtttccg gaaaagctta cgcccgatta gacaaagttg gagcagattc
      901 gtatatttcc cagctgacag tcaaagccaa gacaatggga gaaggagaac agtcggagat
      961 gattgcttct cttaatcaat tgatcaaatg gattgggatc atcattattc ctatcggtat
     1021 cctgcttttt tctcaaagtt acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc
     1081 aatggaagca gcattgatcg gtatgatacc tgaaggactg tacttgctga cgacgattgc
     1141 attagcatta agtgcgacta aactagcaaa gcaaagagtt cttttacaca atatgaaaag
     1201 cattgagaca ttggcaaggg taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga
     1261 aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa caagaaataa tcagtcagtc
     1321 agatcaattg gaagcatgca ttgctgacta tgcaaaagcg atggcaggag acaatgcaac
     1381 aatggaagct gttaaagcat attttacaaa aaccactgat caatcctatc accatgttat
     1441 tcctttttct tctgtccaaa aattcagcag tgtagcgttg gcagacaagg tgtatgtttt
     1501 aggagcgccc gaaatggttt tgcgcgatca attgccggaa tatgcggaag agtttgtttc
     1561 tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga
     1621 accagtattg acagaagcag tcacaccatt aggctatatt ctgattgcta atcctatcag
     1681 aaaagaagca agagccacat ttgattactt caaaaaacag caagtagcca tcaaagtgat
     1741 ttctggagat aatccgctca ccgtatccaa tgtagctgtc caagcaggga tttcacatgc
     1801 tgatcagtac gttgatgcat ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa
     1861 atatacagtt tttggacgcg tcaagccaga acaaaagaaa atcttcgttc aattgataaa
     1921 gcgtaacaat acagtagcta tgacaggtga tggcgtgaat gatattctag ccatgaaaga
     1981 agcagactgc agtatagcga tggcttccgg aaatgaagcc gccatgcagg cctcacaagt
     2041 ggtcttactt gattctgact tttcacgcat gccagaagtt gttgcagaag gacggcgcgt
     2101 tgtgaacaat attgagcgat cagccagctt gtttttagtg aagaatattt tctccttttt
     2161 gctctcactg ttttcagtga tctttgctct gacctatcct ttagaaccat cacaaatcac
     2221 attgattagt ttgtttacta tcggacttcc atctttcttg cttgctctag aagaaaacaa
     2281 aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa aaagctgtgc caggtggact
     2341 aacagatatg atcgtagtcg gtgcgttggt gatctgcggg gtgactttga atctaaataa
     2401 aacagatgtt tctacagcat ctaccatgct tttgattgca gtagggttcc tcgttctcta
     2461 taaaatctgt tcaccgctga ataaattcag aagtcagatc attcttttct gtgctagcgg
     2521 taactttttt tcggtcatct ttttgcataa gctgttttca atcaccggta tctcagctgt
     2581 atcacttttg ctgttgagca ttctattttt ctcggcagat tcgattttta ggcatttgac
     2641 cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg
     2701 cgtagctaaa aaatttctat cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa
     2761 aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc aggatgagtc atccaacttt
     2821 aaaacgcacg agcagttgct cttttttaca ttttcggaat ggtaacggtg aaagtcgaac
     2881 ctgttccaag atgactgtct acagtaatcg taccattaag gagttgcacg tagtctttta
     2941 caatcgctaa ccctaatccg gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt
     3001 aaaaacgctc aaagatccgt tcctgatcct caatgtcgat acctatacca aagtcttgga
     3061 cagaaaacga tagatcatct gttgcctgat agcggataat aatctcgctg tcgctttttg
     3121 aatattggac agcattttca attagatttt tgataattgg ataaaggata tctgttttag
     3181 tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg
     3241 gctgataatt ttgaacgatt tgctggaaat aaggttctgt ataaattgtc tggatctcgt
     3301 aagaattctc gccatctttt gacagctgga tgatttcccg aatcagttta tctaaccgga
     3361 tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac
     3421 catccagtaa agtctcggta aaaccaatca gtgaagtcac aggagttttt aattcgtgag
     3481 agacgttgcc aacgaaatcc ttttgtagct tttctaaacg tcgcactttc gttaagtcat
     3541 acgccacccc tagaatctgt ccggtacctt ctgaattagt aaagtagcgc aaactcaagt
     3601 ctaatgtctg ccgaccttct gtaagggtga tctcctgatg gatcagcgga tgctcaggag
     3661 tcacttgatg aatcagctga atgagttttg gctcttgaat gacttctgtg taccgctgtt
     3721 caggttgata gcttaccacg tttaaatgta gctgcatctt tggattaagc agtagtaaac
     3781 gagaatcagc atcatcaatc aggaatacgc caatcatcag ttcgttcagt aacgtatata
     3841 attgctcttc tgtggtagta cacgcccgat acatcttact catttgttcg ctcaacgcat
     3901 taatcgtctg atacaattct tcccattgat cgggtgtttg catgattact tccgcctgat
     3961 taggattttg caccattttt tttagtacgg gcagcacggt tttcaatggc tggttttttt
     4021 gatagatcag ataatagatc attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa
     4081 agacccaacg gcgaaaactc tcagttctcg gtaaaaaacc gcttgtcggt tctgcgatac
     4141 gtaaaatggc ttctaactgg ccactttttt tgactggtaa tgcgacatat aaaagatcct
     4201 cattcaaagt agtacttttg cgcaaagcgc tacctaggtt cccaccattc aatacggctt
     4261 tgatttcggg acgggagttt cgctgttcat tcaatgtttc attgtttgtg tcaaataaga
     4321 tctttccagt agcatccatt aaagtaatcc gttcgttcga ctgatggacg aattcttcca
     4381 aagcagtttt gttttgcgct gcttggaggt tttcgacatc taattgccgg atcagcagtt
     4441 gtgctttttt tgtcaggtag ctttcttgct gttccacgat ctgctgttga aaataatggg
     4501 aaatcaattg ccagccacca aaaaacaaac taactaaaag aataagaaag aagagacgtt
     4561 cataaatttt tttccatttc atcgtttggg ttcctgaaac ttatagccaa atcctctaat
     4621 agtcaccaag tactttgggt gttttggatc tcgctcgatt ttttctcgca aatgactaac
     4681 gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa
     4741 tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa taaacaagta attcaaattc
     4801 tttcggtgtt agttcaatca gatggtcgtc tacggtcact tgataattgg tcaaatcagc
     4861 agtgatttgt ccaatcgata gatattcagg ctcagcttca tcttttgatt gcttacgagg
     4921 ttcaattcgt ctgaatattg ctttcatacg ggctaatact tcgcgaggac taaatggttt
     4981 ggtcaaatag tcgtcagcac ctatttctag gccaatgatc cgatccactt ggtcatcttt
     5041 agccgtcagc atcaaaatcg gtgtttcatt tttttcttga cgcagccgct gagtgatctc
     5101 catgccatcc atcgttggaa gcatcacatc taaaatgatg aaatcaaatg attgttctaa
     5161 tgctaattct aatccttcgc ctccatctgt ggcactggtt accttgtagc cttctttttc
     5221 caaattgaac gtcaagagcg ttacaataga aggttcatca tcaacaacta gtattttttt
     5281 cattcaaatc tcctttaaat aggtttcatt ctgtttatat tttagcatag aatcagtaca
     5341 aagggctatt ttacaaaaga aataaatggt ataataggaa ggcactttgc tgaatagaaa
     5401 ggaagagcag tcaatgattg gaatcagtgc atgtttaggc ggtctggcct gtcgctatga
     5461 tggacagaaa aaagaagtac cagaattgat ggaactagtt gaaactggag aagctgtcat
     5521 ggtctgtcca gaagtgatcg gcggattacc aattcctaga gatcccgctg aaatcattgg
     5581 tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa gcggcgaaga
     5641 tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat caaaaattgc aggaaaaaca
     5701 cattgatcta gtcatcatga aggaaaaaag cccttcatgc ggaacccatt cgatttatga
     5761 cggaagtttt tccggaataa aaaaaaatgg tgccggagtg gctactgctt attttattca
     5821 gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa actagaggtg agcagaatgg
     5881 aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt ttattcgacc ttattgacag
     5941 aaaaacaaat gaactacatg gaattatatt atgcagatga tttttcatta ggtgagatcg
     6001 cggaagaata cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg acaagcaaga
     6061 tcttggagga ttatgaaaaa aaactccatc ttttttctaa ttatattgtt cgagaacagg
     6121 tattggaaaa tatgactgca tatattacag ataagtaccc ggaagataaa aaactacttg
     6181 aatatgtaca gcaaattcaa gcaatcgaag aataaataaa aggaatgaag aaaatatggc
     6241 atttgaaagc ttaactgaac gtctccagca ggcgatggga aaaataagaa aaaaaggaaa
     6301 agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct tactagaagc
     6361 cgatgttaac ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg
     6421 ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga
     6481 attgacgatc acacttggtt ctgaaacagc agagttaaat aaatcaccaa aaataccaac
     6541 tgttatcatg atggctggtc ttcaaggggc aggtaaaacg acctttactg gtaaattagc
     6601 caattattta aagaaaaatg aaaatgctcg tccgctccta atcgcagggg acgtatatcg
     6661 accagctgcg atcgaccaat tgaaagtatt aggtcaacag ctagacgttc ctgtatttga
     6721 tatgggaact gaagtcagcc cggtagagat cgtccgtcaa gggatggagt tagcaaaaga
     6781 aaagaaaaac gactatgtat tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt
     6841 gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt tgcttgttgt
     6901 cgatgccatg actggtcaag atgccgtcaa cgtagctgat agcttcaacc agcaattagg
     6961 tattacaggg gtcgtcatca ctaaacttga tggggatact cgaggcgggg cagcactatc
     7021 gatccgctca gtgacaggcg caccaatcaa attcatcggt tctggtgaaa aattgactga
     7081 cttagaagta ttccacccag atcgtatggc tagccgtatc cttggtatgg gggatatgct
     7141 gacattgatt gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag aacttgcaca
     7201 gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt gaacagcttg atcaagtgat
     7261 gggtatgggc ccactggaag atctaatcaa aatgatccca ggaatgaatc aagtccctgg
     7321 aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga tggtttattc
     7381 tatgacgcca gcagaaagag caaatcctga tctgttgaac ccaagccgtc gtcgccgaat
     7441 tgctgccggt tctggaaaca gtgttgttga agtgaatcga atgatcaaac aattcaagga
     7501 atcaagaaaa atgatgcagc aaatgtccaa aggggatatg aacatcccag gtatggacca
     7561 aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa
     7621 gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct tcggtacaga
     7681 ctgaaaatgt ctactgccgg agcttttcta tcagcaattt ttattcttta ctaatacaat
     7741 tactctaaac gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa cagaaagtat
     7801 gcggcaatat catgatactg tctggggtgt tcctgaatat gatgaccaaa agctgttccg
     7861 aaaattgatg cttgatatta atcaagccgg attgagctgg cagacgattt taaataaaag
     7921 tgaagcattc gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa attatggaga
     7981 aacaaaaatc agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga
     8041 agcagccgtc caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc
     8101 agagtatctt tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc attcacaaga
     8161 agaagtccca acaacaagtc ctctttcaga tgaattagca aaagatctaa agaaaagagg
     8221 atttaaattt atcggaagca caacaatcta tgctttttta gaagctgtcg ggatcatcaa
     8281 tgatcacctt gacagttgtt tccgcaaaga agaggtcgta cgtatcggca gaaagggcga
     8341 ataaagcatg cgaaaaatca tattttatgg tgctataagt ttagatggct atttggcaac
     8401 gaaaacggat gatcttcaat ggctttttga cactccaaca ggtaagcaaa ctacttatga
     8461 agctttttat gaaacaatcg atacgaccat catgggcaga aaaacttatc aggaagcaaa
     8521 aaaatatttg gagaccgaag caatttatcc agagaaaaag aatttcgttt tttccaccca
     8581 cgcacaatta caattgccag atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa
     8641 aaatctacaa atgacagaag gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa
     8701 accgctattg gaacatcaaa tgattgatga atggtatatc cagattaccc ctgtattatt
     8761 aggggacggg attcctttat tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga
     8821 cacgacacga ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga agtaaataaa
     8881 ataaaaaagg caactatatg cagaaatgtc ctgtacatag ttgccttttt tattaccgtt
     8941 atgcttttta ctctgttaat ttgttagaaa aatctagata cgcatctaaa atcgtttgga
     9001 agaaacttaa tgttccttca acgatattac cattttcatc aattagattc gcaacgtttc
     9061 cgatatatgc ttccggttgc tgcaaggttg gcacattcaa aaagacaagt gattggcgta
     9121 gatggtggtt tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg actaaacctg
     9181 gtttatgatc ccaaacactg tgtccataag gtcttgagcc tacatctaaa gcatttttca
     9241 atactgctgg cacagaacgg ttgtattcag gagtaacgaa aacgacaccg tctagtcccc
     9301 tcacttcctc acgaaaacga gtccattcag caggagcctg ttctggagtt tcgagatcct
     9361 cattgtaaaa tggcagatca tcgattttta caaatacact ttcgtaccct tcaggaagca
     9421 atttcgcaaa agcttctgct acttttttgt tgtaagaatc ttttcttaaa ctaccaataa
     9481 aaaatccaat tttttttgtc atacgatttt tcctccttca aactatctat aataatctta
     9541 caaaagataa gtaactggag caaataaaaa cacttactga caaaagctac atagaagata
     9601 gattacttat ttttaacaaa aaagaagtct aaaatcttct attttctaaa aaaaatcgaa
     9661 tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt tacactacat
     9721 cttgtgaata aaacaatgga ggtggaaata aataatggca gttaaaatcc gtttaaaacg
     9781 tatgggttct aaaaagagtc cgttttaccg tatcgtcgta gctgattcac gttctcctcg
     9841 tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt
     9901 agttttaaaa gaagacttag ttcttgactg gttatcaaaa ggtgcacaac cttctgacac
     9961 tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc
    10021 aaagaaataa ggtgaccgga tatgaaagat ttaagcgact tagtcttaac aatcgttcgt
    10081 ccgctagtca cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa agacttttac
    10141 gagtacaatc taacagtggc tcctgaagat attggccgta tcattggaaa acaagggcgt
    10201 gttgctaaag caatccgcac aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg
    10261 cgtttgaata tcatcgatga taaggaaaag taaataaacg tgctgtactc gaattttcga
    10321 gtgcagcttt tttgtttttt ttattaattg aagaaatgat tatttggcaa aacaagcaag
    10381 aaagcgtatc cttataggag gagggagtga acagccaatg agaaagaaaa tgttatttat
    10441 tccatttgct ctattgttaa gcgcttgtag ttcggttact caagaatcag acaatacaca
    10501 tctatcaagc agtatatctg aaactgttac cagtccagaa aaaagtagta ccaaaacggc
    10561 ttccaccact gaacaaacaa tggctacctc aaataatgag aaaaagactg ctttagacca
    10621 gttgaaagaa caacagccaa atgttcccat gcctctagat gtaccagtct cttcaggcta
    10681 tctcaatatc gcagcgacac atacaaagca aggttattct attttatatt acagaacaga
    10741 tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa acgcctattg caacctatct
    10801 atatcaatat gggtttgctt ctagtcaaga aacaatccaa gttttgcagc catttgagat
    10861 cgacacaaat ggtcagcaag tggacttagg ttctcgaatc acagggtatc agcaaggtgc
    10921 agcaggttca agttttcttg aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa
    10981 taatatagaa ggccaagatc ccttactatt agctaaagaa atcgtagctt atttggaaga
    11041 aaacagtcta ccagcacctg aacaatttgg taaaattact gttgatatgg gtgatacgac
    11101 gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat gcctatacga tcactcatca
    11161 agatcctatg tctgcgttga agatggcagt ttctatgaag aggttgtgaa atcagcgcgc
    11221 tgatctgagc ccatcattta actccaagtc acaggaagta atcaaaaata gcacgccgca
    11281 tttttgcata attttggctt aataccttaa aagaaaacta gaattgcctc ttcacacatg
    11341 ctaaaataaa acagacaaaa aagaaatggg gatataagtt gactgaatac ttgaatgtag
    11401 gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg tgttatctca acaacggatt
    11461 tcccagaaga acgttacaaa aaaggaacgg tgctaacact attccaagac ggaaaagcac
    11521 ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa ttttgactta ttgagttttg
    11581 aaggacatcc ttctatcaat gatgtggaaa aatatcggga tggcatatta aaagtgtcaa
    11641 aagacaactc tgtagatcta gctgaaaatg aattctatta ccatgagatc atcggtctgc
    11701 atgtattaga agatggaact gagttaggta aagtaaaaga aatcttgtca ccaggtgcca
    11761 atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca tatatcgctt
    11821 ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg tgtagaaatt ccagaaggac
    11881 tgattgacga tgaagattga tgtattgact ttatttccaa gaatgtttga aggtcctatg
    11941 ggggaatcca tcatcggtaa ggctgtagat aaaggattat tagatatcaa tatctctaac
    12001 ttccgcgatt attcagataa caaacatcag acagtagatg attatccata cggcggtgga
    12061 gcaggtatgc tgctcaaagt ccagcccatc tatgataaca tccaagccat tgaaaaagct
    12121 gcaccggatg tcaaaaaaag agtaattctt cttgacccag ctggtaaacg attcgatcag
    12181 aaaatggcag aagaattttc acaagaagaa cacttagtat tcatttgtgg tcattatgag
    12241 ggatatgatg aacgtatccg cacattagtg acagatgaag tttccttagg agattatgtc
    12301 cttactgggg gagaattagg tgcgatggtc atgatcgatg caacagttcg gcttttgcca
    12361 gatgtgttag ggaatcagac ttcagctcaa acggattctt attcaaccgg tttattggaa
    12421 catccccaat atactcgccc ggcagaatat aaagggatga aagtaccaga agtattgaca
    12481 aatggaaatc ataaattgat tgaagaatgg caattaaaag aatcactaag aaggacctat
    12541 caacgtcggc ctgatctttt ggaaacactt gaatggacac ctcaaatgac gaaattttta
    12601 gaagaaatca agaaagaaga acaagaaaaa acagcagatg aagccaagtg actttttcta
    12661 aaatcttctt tacagtaggt tgataatatg ttagtatttt atggtgagtg caaaagcact
    12721 taactattac gatattccgc tgtgagaaaa gctcataaga atatttggaa taaggagaag
    12781 aaaaccatga atccattaat cgaagaatta acaaaagaac aattacgttc tgacatccca
    12841 gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa
    12901 cgtatccagt tatttgaagg tgttgttatc aaacgccgcg gtgctggaat cagcgaaact
    12961 tatacagtac gtaaagtttc taacggagtg ggcgttgaac gtacattccc attgcataca
    13021 ccacgtgttg caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac
    13081 tacctacgtg cattacacgg aaaagctgct cgtatcaaag aaatccgtcg ttaatcattg
    13141 ttatcagact gagaaagaac ccttggtatt caagggttct tttttgttat ctgttttttt
    13201 atattaacga tttctatttg gtacattttt gattaataaa aaggggcaaa aaaggggcaa
    13261 caagttacag tttttctata tcttctttca atttttcttt tactcctttt gtcacatgca
    13321 aataaatctt ttcagtgata tcactatttt cgtgaccaac gcgatcctga atagcataca
    13381 gaggtgttcc tagttccgct aattttgaaa tatgggtatg tctaaaaata tgtgaactaa
    13441 gttttttatc aatcttcatg tcagctttat gatttctcaa ataggtgtta attgctgtta
    13501 gttggaaagg agttcctttc gttgtttgaa atagaaattg accattcgga tttaattcta
    13561 gaagctcatt gtaaatagtt atcgcttttt ttggtaaatc aatttctcgc attccagcag
    13621 cagtttttgt agaatcagat tttttcatat cagctattga acgttctcta tacatcatcg
    13681 tcccatttat aactactgat gcattattat tggtaatgtg tacatcgtct ttgcttaatg
    13741 cgattgcttc accaggcctc atcccagtca aatatagcca ctggaaaagg agagaatatc
    13801 gtttattgtg tgaagttgtg aaatctacta gtctattata ttcatcatct tctaaaaact
    13861 tatctttaat ttttatcgtt tttgactctc gtttataatc aataactact tcgttgatag
    13921 gatttttttc tatatagcct tttttcatag cgtatgaaaa aagaagattc aatttggatt
    13981 taattacact gacgtactta tttgacaagt catctttata tatcatgtct tcaaaaatat
    14041 tatttaaatc tattgtatta ataccagaaa cgatgtaagt ttctggtatc ttcttcttta
    14101 tagtgtttaa gatattattc gtaggataat atgtagactc cttaacttgt cttttgtaaa
    14161 taactaacca ttcttcgaca agctcatgga atgttagatc aggcttctgg agtgtctttt
    14221 catttagttt tatatcaatt tttttattta actcaagtaa tgccactttc tgagtttctc
    14281 gagatttatt tttataagta atactcactt tttttctttt tttagttttt ggatcaatat
    14341 atcgctcatt atatttatat actttttttc cgtttttatc agtttttgtt tcaatccaca
    14401 ttgttttcat ctcctatttg ttgctataat aggaatggat aagtaagcca attatagcag
    14461 gtttattttt catcacgtcc acaaactttg gcgagggagg gggcgtgctt ttttagtttt
    14521 gttttagagt tggtgtattt atatgccaat cgtttgggaa acccaaactt tttaaaatag
    14581 tattaatatc gatagtattt agcctgttat ccaaagtcga aattcttttt ctgatagtat
    14641 tgtgcaattg agcgtattga agtttactga gaaagcactg cagacaaaga aagacgttgt
    14701 aagtattgtt tctttgggaa tttttggaaa ttccatatat agaatgtaaa tctcgataat
    14761 atttaatatc ggatttacat ttagaatcta ataatctgtt attatgggca caaatattac
    14821 gtatttgtct gatattttct gtaaaagata tcattatttc cggcgtaaaa ggggctgtca
    14881 tatgaatatt ttctgatacg aaactgcaga tattttttgc tatgttgttt tgtaaaaaaa
    14941 ctggtaaatt tttaattaag gtattcattt ctccaaaatc aaggtaatct atagaaccca
    15001 tataggtacg tcattatgtt tttttacata atgctttata gaattatttt ttttactctt
    15061 cttattggaa ttaatgattt tcgttaacct tgaaattaac catcctaact ctaaagtttt
    15121 tgaattatca taacaattaa tatcaaggta tgcatatggc ttattattat aaacctctgc
    15181 aaaacgatat gacaaaatgc ttttgatatg gttttctgcc tcagtaattg cgcggaaaag
    15241 agcgtgctta atctctttgt caaaataata taaatgagtt atctcgtcaa aagtagtacc
    15301 tggcaaataa ttattttgat tgctcatgaa atatttacta tatccattaa ttatgttgta
    15361 ataattgttt gtaagtagat attgctttgc tctttggtaa tttggaataa taactccacg
    15421 gttgtttagt attgataatt gcttatgcaa attttcgaaa tttttcaaca aaaaaacctc
    15481 catccatata gaatgaaggt tttcgcgttg gtccccgtag agattccaac acttatctct
    15541 taatttcatt ctatagggtt ttatttaata aatcaactgg tttgcaaaaa atattaaaat
    15601 cctttagtgt ttttcataga acacgcttgc tttggacggt gggcgtgctt ttttatttaa
    15661 acatattcat tatgaaccaa atgcaaaata aacccaatgg caacataaat ataaaacaac
    15721 caatttgtga gatgaaactt ccagtttttt ctatacgttc accagatttc tgcatctttt
    15781 ctccggctct gtatagttta gaatttgcta atcttgattc atagttgtaa aaaacttgat
    15841 gtacttcttc aggagaaaat tttcctccac aattagggca ttgggttgtg ttgaaagtta
    15901 aaataaagtc tttcttacaa aaattacaat ttatttttat ccgctctttc tccaaatcca
    15961 tttttaactc ctcatttcta tggtatgatt ttactggaga atcttagaaa taaggtttct
    16021 agtccgtgtt gcagcacggg cttttttctt tataactttt ttagagatta taggcaaaat
    16081 agtagggcat aaaaatatat tattgaattc cgtatttaga aaatcctaat tgaacttcac
    16141 cggaagtctt ttgctgtgtt gtacgcaatg cttcttcttc agacattcca ttctgtactt
    16201 tccatgcaac aggcgacatc ccatatttgt taacaaaatc agtaagtgat aaagtgtcag
    16261 cgtcttgctg agcgcttgtt tgttggtctt ctggattttg ttgagatgct gcttgttgtt
    16321 ctttctgatc ttgactgata atattgccag catcatctgt agtcaatcca ttttcataaa
    16381 gagccacgcc gaaagcttcc cactctttgt tggaccaatt tgcacgatca gctggagttg
    16441 actgtaaagt gcgttgtttc atctgttcat atgtttcttc ttgaggtgcg gtttggattg
    16501 tatcctgact ggagcttata actgttgggc taggttccgc cgtagcttgg ttggagcttg
    16561 taactgttga actagaatct gtcttagatg tagatttgct agtagaggaa ctggtttcag
    16621 ttgcttcttt tgttttactt acttttgttt cttggttaga agtggcatct gttgattcag
    16681 cttttttatt atttgaacaa gctgaaagta gcagagcagt acttaacaac aacataacgc
    16741 taactttttt cattttataa ttcctctttc tcgttgtaat atgtgtgcta acacgggctt
    16801 ttttatataa ggaaacgata agcgctttct ggaagtccgt aaagattttt taattcctcg
    16861 atttttctag gatattgatc attgtcttct ttgtatagag aaacaataag attagcagca
    16921 aagcaattag cttcgctttc agatttgctt ctagatgttc ttgttgatac gtaatagcta
    16981 gataagccac gatgaaaaat agcgtgacct aattcgtgag cgcaaatgta gaatctttcc
    17041 tcagagtctc gcagttcatc atttaaaaag attatcgcac gatctctaat ttcttgaaac
    17101 tgtcctttgg gattttcgat aaaaggaacg tattgaattt taatgcccat cttttcacaa
    17161 atataaaaag gattagcgga ctggtatttc cgcttcaact cctcgactaa attaatcgta
    17221 tccatctcca taagctcaca tctttttgcc tttttctttg tcttctttca caatatccca
    17281 gaaggttcca ataaggatat cttttacgcg ttggacctgt tcaggcgtca gtgtttctcc
    17341 gccataagac atattgacat ttgaatctag taatttatca agttcaacta cttcatcttt
    17401 tgttgcccag tcaggaactt ggtttctacc gagtaaataa tcagtagtta cattaaaata
    17461 atctgctaca agttgtagtc ttttagtact aggagttcta tttttccatt gataaatcgt
    17521 gtttttaggt atatttaatt cttcttccag ttgagttata cttatgcctc ttttatgagc
    17581 taattccttt attctatcta gtaaattcat tttcgcacct cacaagctac gaaaacacta
    17641 ataaaaaaat tagttaaagg tgttgacaac taataaaagt gttagtatac tgttttcgta
    17701 agctaaaata ttagctaaaa agacttgata actaataaac acttcacggt cggcaaactc
    17761 tgaaatgtaa attactaggc atttctgtgt cttatttagc tatgtctata tactaataaa
    17821 aatattagtt attgtcaacg aatattagct aattttttag cttacagatt attttttaga
    17881 aaggagctat ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa
    17941 gaagaatgac tttcaaagaa ctagctgcgc ttgttggtat ttcaggagct tatttatcag
    18001 atattctaaa cggtaatcgt gatggaaaga aagcacaaca gcatattgaa acagtgaaaa
    18061 aaatattgga catccgatag ggggataggc gatgacaaaa ctaaaaaaac aagattttgt
    18121 aaaaaaatac aattattctc catctactta tcaacgtcga atgtcggaac taaaaaatac
    18181 agcaattttc tcagcggcgt atgaacgggt cacaggacaa gaagtttgga tcaatacaga
    18241 attatacgat aaatttttgt ctttcaaatc ctataacagg ttacgcacaa gaaaggtaac
    18301 acctaaagaa tttatcgaga agcatttagt tgatttataa aaaacagaac attttgagag
    18361 gtgaaggtta atgggtaaat tcaacagagc attagtattc agcgcgcccc taatcatcta
    18421 cgctttagga ctttggggaa gcagacaagc gttgataggg acgattgtgt acatggtttg
    18481 gatttttata ggtcttgatg aagctgagta caaaacaaaa aagccagtcg ggagggactg
    18541 actaaaaatg aatagaaaag agaagataga atgtggatta attttactca gtttaattct
    18601 ttcactaatt tctctactgg gaagtttgtg cttttaatgt caacaacttt atggatttga
    18661 gtccgattta gagtaaatat catttttgtt tggtgattta acacttcctt aaaattaatt
    18721 tttcctacct caaatgcaag gtaaaaagac atagcacttt taggaggaat gtttagcggt
    18781 acagattcag agtatatcct attatcatct aatcctccct tagcaattaa ttttttaaat
    18841 ggagtagctg gccatctgcg atccaactta gaggatgaaa gttctaaatt aactaacata
    18901 gcaggttctg ttgaatagtt agagatgatt acttttgtat aaatcatatc cgcagcgaag
    18961 taagaagcat taagttcgac atctagttgt ggacgcattc ctttaatttt tacacctgct
    19021 aaaaacgtac tcaatataaa acttattatt gacatccatt gaaaaattgt taagtgaaga
    19081 aataccaaaa taaacaccac cggtttttaa aataattata tcaaaaggag agaagaaata
    19141 atgcaagaat tagtaatttt gaaaaataaa gaagctgtaa ctacgagctt acaagtggca
    19201 gaaagtttca aaaagaaaca taagcatgta ctagaagcaa ttgaatcaat aaaaagatcg
    19261 gtcgaaaatt cggccaatgt tgaagatggg tccaattttg gacagatgtt tgtggaaggg
    19321 aacgagccag actcatacgg aagaagtcgg agagtttatt tcatgaatag agacggattt
    19381 tccttgctag ctatgggatt cactggaagt aaagcaataa atttcaaact aaaatttatt
    19441 gaagctttca acgaaatgga agatgttatt cggaagaata ctgttcctca aacaattgaa
    19501 gacatgatga tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt
    19561 aaagatacta tgcgaattag tggacaacaa gagtttgaaa ttaagcaaaa aggaaatata
    19621 aaagttatgg aagttctagg ggggaaagaa agtcgagctt atgaagaaat cagcaaaaaa
    19681 gtattctcaa aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag
    19741 ttacctcgta agagattcga tgatgctgtt tcatttattg aaatgtggtt accagaaact
    19801 gggatccgca tggaaatcga tcaactgaac agacaacaaa gacttttcgg tgatgaaaat
    19861 gaatagagct gaagcgctaa gaatagggac gttaattgct aatcgctggt ggagacacaa
    19921 taaaccaaac atcctaagcc gacaacatat cgaaaagaaa aaagcatggc aacaaataaa
    19981 aaaagtgact cagccgacca aagcaatgag tcactcaaac aaatcataac taaggagatt
    20041 ttaacacatg gaaaaagaac tttccactct agatcaatat ttgactgatc ctagttgggg
    20101 caaatcgaat atcaaggaaa caaacaaccg aaaaatcaga cgaaatcttt tgacagatga
    20161 agaactagaa tgtgatcaag acgatttggg caattttgtg agtatttggg atcatgttta
    20221 ccttatacat ctatcaaaac attcaaacaa acctgaatat atttacgtca tcgaagatgg
    20281 cttgactgat gca
//
LOCUS       DACGLH010000045        20284 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.048, whole genome shotgun sequence.
ACCESSION   DACGLH010000045 DACGLH010000000
VERSION     DACGLH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.048"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(157..1278)
                     /locus_tag="IXT21_001824"
     CDS             complement(157..1278)
                     /locus_tag="IXT21_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ4869211.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1403..2344
                     /locus_tag="IXT21_001825"
     CDS             1403..2344
                     /locus_tag="IXT21_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ4869212.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYNPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(2494..3390)
                     /locus_tag="IXT21_001826"
     CDS             complement(2494..3390)
                     /locus_tag="IXT21_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ4869213.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            3659..4999
                     /locus_tag="IXT21_001827"
     CDS             3659..4999
                     /locus_tag="IXT21_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4869214.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVIVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            5217..5660
                     /locus_tag="IXT21_001828"
     CDS             5217..5660
                     /locus_tag="IXT21_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ4869215.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(5925..7343)
                     /gene="nhaC"
                     /locus_tag="IXT21_001829"
     CDS             complement(5925..7343)
                     /gene="nhaC"
                     /locus_tag="IXT21_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ4869216.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(7746..8969)
                     /locus_tag="IXT21_001830"
     CDS             complement(7746..8969)
                     /locus_tag="IXT21_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ4869217.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(9000..9839)
                     /locus_tag="IXT21_001831"
     CDS             complement(9000..9839)
                     /locus_tag="IXT21_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ4869218.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(9856..10536)
                     /locus_tag="IXT21_001832"
     CDS             complement(9856..10536)
                     /locus_tag="IXT21_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4869219.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(10538..11611)
                     /locus_tag="IXT21_001833"
     CDS             complement(10538..11611)
                     /locus_tag="IXT21_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4869220.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     gene            complement(12257..12928)
                     /locus_tag="IXT21_001834"
     CDS             complement(12257..12928)
                     /locus_tag="IXT21_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4869221.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(13015..15693)
                     /gene="mgtA"
                     /locus_tag="IXT21_001835"
     CDS             complement(13015..15693)
                     /gene="mgtA"
                     /locus_tag="IXT21_001835"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ4869222.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVDGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            16352..16789
                     /gene="ndk"
                     /locus_tag="IXT21_001836"
     CDS             16352..16789
                     /gene="ndk"
                     /locus_tag="IXT21_001836"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ4869223.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVTGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(16811..17677)
                     /locus_tag="IXT21_001837"
     CDS             complement(16811..17677)
                     /locus_tag="IXT21_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4869224.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(17837..19930)
                     /locus_tag="IXT21_001838"
     CDS             complement(17837..19930)
                     /locus_tag="IXT21_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ4869225.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
BASE COUNT         5902 a         4180 c         3568 g         6634 t
ORIGIN      
        1 caagccaatc ccgaaaatct gcacactgct tccttgtctc cacaaggtac agtctgaatg
       61 cagctttcat caatcgactt aatttttcaa tgcacggatc cctcactttg tcctatattt
      121 gaaattacga tagcataaag aaaatgattt tgtcaactac ttcgggagga tggtgaaaaa
      181 tctgctaagt gtctgtccta agatttttgt tttttctaat gtttgcctgt gttccattgc
      241 ttcttctaaa cctattggac ccaaatgaat acttaagaca attgggagaa aatcagagag
      301 tccttctagc tcttggtctc tacttccgca aatagcgatt attggttttc gatatttttt
      361 tgcaagcact gctacaccgt gtggaacttt tccgtggatc gtttgctgat cgatccgccc
      421 ttctcctgta atgatgacat ctgcttgttc gatctttttt tccaatccag tgatttggct
      481 gactaaatcg aaaccagaaa ccatcttcgc tcccaacaaa aaaagggctc caccaatacc
      541 gcctgctgct ccacttcctg gaatatcatc taaatccacc tcccagaaat cttttatttg
      601 ctgagcaatc tttcctgctt gtttatctaa gaaagctagc gtcgtatcta ttcctccttt
      661 ttgtcttcca aattgccaag cagctccttg ttgacctgta tatggattgg taacatctgc
      721 agcgataatc aattttactt tagaaacaag aacgtttctt cgatcaaaat gaagtttttt
      781 agtagataat aaaggatttc cttctgtgta cccttctaaa gaagcaccca aggattggag
      841 taatcctaga ccaccatcgc tacacccgct tcctcctaaa gtgacaataa tttgttctac
      901 ttcttgaagc agtgcatctt tgataagttc tcctaaggca taactggaag caagtcggat
      961 agtttgatcc gttggtttat caagtagatc taaccccaat gcttgtgcag attcgatgac
     1021 tgcggtattt tttccttgcc aagttgtcaa taaatagctg atttttcgct gacggaataa
     1081 aagatccatc ccatcaatgg tacgccatgt tcccccgagc gcgtaataga ttgcttctgc
     1141 agtattttct ccgccatctg caatcggcac attgattttt cttcctttac gatcttccca
     1201 gccatttaga actgcctgat ttagttcaaa tgaagaagca cttcctttaa atgaatcaat
     1261 tgccgccaaa atatccatct gtatccctca ctttcttctt aagtaagaca ctttttttca
     1321 tttttttcaa acaaaacaat gctattttct tccatttttc tatatactaa aagggaactc
     1381 attgataaag gagagaaaaa acatgaaaat cgcaatagct ggagccggag caatgggcag
     1441 ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc
     1501 tgcacacatt gaagccattc gtacaaatgg gttgattgct gactttaacg gtaaagaagt
     1561 ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc gaaagcaacg aacacgtgga
     1621 cttgatcgta gcattaacaa aagcgaatca attagatgat atgtttcgtt cgatccaatc
     1681 cattattact gacaatactt atgttttgtg tttactaaat ggattaggtc atgaagatgt
     1741 actggaaaaa tatgtgccaa agaaaaatat cttatttggg atcacgatgt ggacagctgg
     1801 tctagctggt cccggaaaag tcacattgtt aggtgatgga gaaatcgaac tagagaactt
     1861 ggaaccagaa ggcgaagcat ttacaaaaaa agttgtggaa gtctttcaag aagcaaaatt
     1921 aaatccgatc tatagccata atgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg
     1981 aacattgaat ggtttatgca cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa
     2041 aacggcagag agcatggttc gaacaatcgt atctgaattt gcttctatcg cagcaaaaga
     2101 aggaatcata ttagatcaag aagaagtgta ccaacatatt gcttctactt ataatcctga
     2161 caacatcggg ttgcattacc catctatgta ccaagacctg atcaaaaacc atcgtttgac
     2221 tgaaatcgac tacatcaatg gtgccgtttg gcgaaaagga caaaaatatg atattgctac
     2281 accatattgt gcctttttga cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga
     2341 ataaaaggct ctctgatgag aatcagctga atcagcagct ggttctcttt ttttaagccc
     2401 aatgaaagat caaaaagaaa acacaactct catgatgcca taagaattgt gcttgtttgt
     2461 ttcggtacat aatctcttca aaatgtaggt tttttattta ttttcaacag aggagaggtc
     2521 atattcgtat tgctcataaa tagtggaatc gttagtgata aatcgttttg cccaattttc
     2581 tgcatcataa aattccaata gctctgatgg gattggctta aaaggtgtat gcagaaattc
     2641 cggtaattca tctaaatcac gttctcgaag caaaaaaata ttttcaggac gataaaaatg
     2701 ataatgagca atcgcttgat tatccgtcac tagtttcttt tgaaaataca tagattcaaa
     2761 tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc
     2821 ccctgttttt ttcaggtact cttcataagc aagatatcca ggttgttcat tccctcgatg
     2881 tggaagaatg aaatagttgg tcgtaacatc tctttttata aattcctgct tatagctttc
     2941 tgctcgttct tttctcccat tattcgttgc accaaaaaat agatccgtct ttggttcgct
     3001 agaagaaaaa tccatatgtt tcgaataaaa tgaactatta tgtttcagcc caagttcttt
     3061 tgcttccaaa aaatcaaaat gataaatctc atcgattgcc ggttcatctt gtgcctgctt
     3121 cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa aataacacaa tttttgcatt
     3181 ggtaaatggg cgcacgcgtc gagccatctc catattaaat ccataatcta aaaaaacgat
     3241 cagctgataa tttgccaact gttgtctagc tgatttaatg aattccgact gtagtaaatc
     3301 ttttccggac caaacatcta aagagcggat ggaaggcaaa aaataaagtg atttattttt
     3361 tgcaactagc agtacgtcat ttttgttcat cttttccgcc tcactttctc tgtcactttt
     3421 tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa cgcttcctat atttgtatga
     3481 aaatttaaca tttgtatgaa aatatgatga aataaatcat tcttctatta aaattcgcct
     3541 aaaagttttc tgaatagttg taattgtctg aaaatttgtt atcattagtc attgttaaat
     3601 attgctattt attcttaata taaattttga ttgtgaattt aaaagaaaga ggtcatttat
     3661 ggaagataaa aaattgaaaa aacaacttag ctccagacat atcaccatgt tagctttagg
     3721 tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga tcgccggccc
     3781 ttctgtattg attgcttttt tgattggcgg gctgatttta tatatcgtga tgaaaggtct
     3841 gggaaaaatc gttttaagcg gtggcgacac gcatcacgga ttatctggtt tgatacgccc
     3901 ttatctaggt gctcattctg ctgattttac tgattgggtc tactggtcta tgtggattat
     3961 caatatcatc gctgaagctg ttgcagctgc ttcctttttg cagttgtggt tcccgcacat
     4021 tccagcctgg gtatttgttt ttatgttggc tgttttaact acgatcatca atctttattc
     4081 ggtacggtta ttcgcagaaa cagaatattg gctggctttt gctaaaatca gtgtcattat
     4141 cttactaatc atttttgcta cttatcttgt cggtcagcaa atgttgggca caggcgtttt
     4201 ccctaccctt cagcaattga ctgaccatgg tgggtttact cctcatggta tgaagggaat
     4261 catcagttct ttgcttgtag ttatctattc ttacggcggt tctgaactga ttgctatcac
     4321 ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa gccatcaaag gtgtcatggg
     4381 aagaatcatt tccttttaca tcattccttt gtttttgctt ttgattcttt ttccttggaa
     4441 tactttagca ggcacgaatg tcagtccttt tgtgattgta tttgaaaaaa tgaatatccc
     4501 atttgcagcg gatatcgtca attttgttat tgtccttgct ttgttctcat ctatcaattc
     4561 gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa aaggttcctc
     4621 tagaaaacta gctgttttaa acaagcatca agtaccgcaa cgtgctgttt tattttgtac
     4681 cagtgttctt tatcttggtg tagctctttc ttattttgtc ggtgacaaat tatttggcta
     4741 tctggcaggt tccctttcgt atacagtatt attgatctgg atcattatca gtgcggcatc
     4801 ctttgtactg gcactaagaa aaggaacatt atgggataaa ggaatcagct tttttgcatt
     4861 agctgtctta ggattgattt tccttggtat cctgttcacg aactccattg gggtaactgt
     4921 attgactggc ctgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcgtt
     4981 cgtattgacg aatgaatgaa atcgatagaa aattttattt tatttatgtg gaatagatgg
     5041 aactattgaa gaaaggagtt gtgacaaaag tcttgttgca actccttttt ccgaataaac
     5101 agtctcatga cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt
     5161 tctccatgct catgacatga tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga
     5221 aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg tttggtatga
     5281 tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag tcgtacacga gtattcatgg
     5341 cgttgatgat gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg
     5401 aaaacaaaaa gataaatgtt atgatcttag ccgctagtct tcttgttttt gccggttcct
     5461 tgtttatggt acgaagccaa actactgtca acgacacagc ttggatgaag gccatgattc
     5521 ctcatcattc tatcgctatc ctgacaagta aacgagctga tttaaaagat cctgaagtaa
     5581 aaaaattagc ggaagaaatc atccaggcac aagaagaaga gatcgacaaa atgaaaaaaa
     5641 tgatcaaaga aatggaatga ccattatccg tccaaataaa aaaagaggat gttccagaag
     5701 tgatcagctt caagaaataa gaagaagcta cttctggaac accgtgaata aggggttgtg
     5761 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcctatttt
     5821 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttcgtaaaag
     5881 ggattgtgac aaaaaaagtc ttgtcacaat ccctttttat gtttttattg ttctaattgc
     5941 gctgtcttgc ttactgcttc ttgcggtttc acaaattttt ttgagattcc ccaacctgtg
     6001 aaggcactga ccatcgaaaa cacaggagaa agcaaactga aaaatacgaa tggcaagtaa
     6061 gtgagtgtag gaataccaaa tgtacttgct acgaaacttc cggcaattcc ccaaggcact
     6121 aagtagttga ttactgttcc accatcttct agcgttctac ctaatacagt tggatcaaat
     6181 cctgcttttt cataagtttc tttaaatgca ttaccaggca gaataacaga taggaattgt
     6241 tcaccaataa aaatatttac tccgatactt gtcatcaatg ttgtaaaaat caattttggt
     6301 gtgttcgtca tctttcttga tactcgttcc atcacggttg agatcaagcc aaattcgatc
     6361 aacaatccac ctaatgacaa agttagaata atcaatgaca cagtaggcat catactttcg
     6421 attcctccgc gactaagcag tgaatcgatt tgttggtttc cagtatttga aacaaaccct
     6481 gattcgatga ccccagccaa cttcgttaag gaagtttgtg ggttttgaat aaaaatcatt
     6541 cctactgcta cagcgatatt caacaaaatc gtcaaaattg ccggcacctt tttccatgca
     6601 cacagtaaca ttaaaaataa aggaatcagt gaccagattg aaatagtaaa gtttgcttct
     6661 agaacatcag tcacttcacg aatagatgaa agacttgcgg ctttacttgt gtgtcctaaa
     6721 atcatgaata aaaagaaaga aacaacaaat gccggaacag ttgaccacat catatgcttg
     6781 atgtgtttga acaaatcggc atcaacaata gctgctgcta gatttgttga ttcggataat
     6841 ggagacatct tatctccaaa aacggcacct gaaacaatag ctccaacaac cagcgctgga
     6901 tttaggccta gtgttgtccc aataccgaaa aatgcaatcc caatcgttga cataacagta
     6961 aaggcactcc ctacagcgct acctacgatt gcacacacaa caaatacaga agggacaaac
     7021 cactggacac tgatcaattt gaacccaatg accattagag ttggaatgat ccctgcttgt
     7081 atccatacag caatcaatga acctactaaa ataaaaataa agattgggat gattcctggt
     7141 ttgatgccat tgatgattcc ttcgtgcagc caatctgtcg ggtatctttt tatcactgca
     7201 tacaacatga ccattccgat cgctgctaag atcgtgatat taggtgagac ctttaatccg
     7261 acaacgccaa tcgcaatgcc taaaacaata accattaata caagtgttgc ttctaaaaaa
     7321 cttggtgttc ctttttttcc catgataatc tctccttttt tagttgttct tatgttctaa
     7381 aaaagttaag atatcggatt actccgccgc aattcatccg ctattcctcc tttataaaaa
     7441 taaaaagacc tgttttaaac taaggacgaa tgactccgcg gtaccacctt aattaaaaac
     7501 agatctgttt ttcactttca aacttttatt caaatgtatg gactttccat gtaattcgtc
     7561 aattaggctt gatcggttca cagcgaccac cgactttctg aacaaaacac ctaaccgcta
     7621 ctgcgtgaaa agttgacttg tatgaagttt accttccata agatcatttg tcaatgaaaa
     7681 agaaaatatt ttttcatttt tctgaaaatt atttttataa ctttcacaca caagtttatt
     7741 ttttactaga aagtagcacc ctcttgtttt tttcttgcta ctatttcttg atgttcttgc
     7801 ttgattcttt ctaatagctt tggctctttc aacagatcaa gagctgtatt ggcgagtagt
     7861 tctgctgcaa tcgcaataga agcaaggcct ttgtcacttt tagcagcagc tttgaattct
     7921 tctgtgtgtc cggcaatata tgtctcagag attgctaccg taggctggat tgttggtatg
     7981 acttggctga catttcctac atcagaagaa ccgagacttg ttctttgttc cgtttctatt
     8041 tcttcatctg gaacacctgc ttcttttgca tgctggaaga ataagtcatc aaaagaagga
     8101 gtaacgatga catcatcaac tgcattttgg aacaatccaa actcaaaagt cgtgcccgta
     8161 cttatcgcag cacctcgtac gatattttcc actttttcat atactttatc taatgtttgc
     8221 cgatttcctg cacgtaaata gaaacgtcca gcagcgtaat ctggaatgac gttggcagct
     8281 actcctccgt ctgtaatgat cccatggata ttgacatctt ttggcaattg caagcgtaga
     8341 gcgttgattc cattaaacac ttggatcaat gcttccaaag catttactcc tttttcaggt
     8401 gctgctgctg catgggaagc gacaccgtaa aatttgatgt cgactggatc attcgctaaa
     8461 gattctgtcg ttttcccata acgaaaagct ggatggacgc ataacgctac atcgacatcc
     8521 tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc cgttctcgcc cccttcttcc
     8581 cctggggtgc catatacacg gatttctcca ccagtttcat caataaattg ctccactatg
     8641 ctggcggcta atacggagta agtaccaaac aaattatgcc cgcatgcatg accaatacct
     8701 ggtaaagcat cgaattctgc caaaaaggca attgtgggtc cgggtttatt ggaaatataa
     8761 cgtgcatcaa aggctgttcg gtgtccagct acttcttttt ctacccaaaa cccttctttt
     8821 tctaattgtt taatcaacgc tgctgaggaa taaaactcat agttacttac ttcaggatgt
     8881 tcgtggatat ctagagcaag tgcttgatat tctggtaaac gatattggat tagatccttc
     8941 aattggtttg ttgagattgt tgttgtcata aaaaattcct cctatgctag tcatttttct
     9001 tattttctcc cgaatgtttc ccatgcaggc acgcttgatc cttttgatgt tttctcgatt
     9061 actttgactg tgtcttctgt ttgataagcg tctactattt tttgatacgt tttatttttt
     9121 tcatcttctt ttcttgcaac gatgatattc acataaggac gtgcatggtc atccactggt
     9181 tctaagaaga tggcatcaga agcaggatta aatccagcat ctacagctac accgctattg
     9241 atcactgaaa tgtctacatc tgacaaagca cgtgctgttt gggaagcatc taattcagaa
     9301 atgtctagat tttttctatt ctcggtaata tcagaaacag tgggcgcttg tccttttgcc
     9361 ttatccactt tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccatttgta
     9421 acatcattag ggattgctac ttttgctccg tctttgatat cagctacatc cttcaaagtg
     9481 ttagaataaa ttcccagcgg tgcattgaca gtatttccga tcgatactaa atcagttccg
     9541 tgttctttat tgtaattgtc taaaaagaat tgatgttgga aagaattcaa atcaatcgat
     9601 ccatctgcta aagccgcatt tggttgattg taatctgtaa atttcacgta ttccaaatta
     9661 atgttctcct ttttcaaacg gtctttcacg ttgtcccata catctgtatc ttctccaata
     9721 attcctagtt ttacggtagt ttcttttttg tcagttgtat ctgcttttcc ttggctacac
     9781 cctgccaacc aaataattgc tacaaatgta atgactacaa aacgaataat ttttttcatt
     9841 ttttctcctc cactttcaat gtgtaatttt tttaatcagt tgattactga taaattgact
     9901 taaaaaaact aagattagaa tcaataatgt agccacaatc gttacatctg tttggaatcg
     9961 attataccct ctcgatatag caagattccc taatccgcct gcacctactg ctccagccat
    10021 ggtagtcagt ccgatcacgt tgatgatagt caacgccgag acccgaatga taccaggcaa
    10081 accttctaac aaatacacac gtaaaataat accgattgga ctggttccca tcgcttctgc
    10141 cgcttcgatc accccaggat caacttctag cagtgcgttc tcgatttgac gagcaaaaaa
    10201 cggaacaact ccaaaaatca atggcactaa agctgctttc gcaccaatcg tcgttccaac
    10261 taagaaacga gtagtcaatg ctaaaagaga aagcaagata atgaaaggga tggaacgaat
    10321 gatattgatc agcttatcaa gtatttcaaa tagcagacgg ttagctagta atccattagg
    10381 gcgtgtcact actaacgtca cacctaagat gattccgatc atcccgccaa tgaatgctgt
    10441 ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca aattcatccg gaatcaaata
    10501 aacattgggc aaatatgttt taaagaaatc agtcatttta tcttcctcct tcgatcactt
    10561 tgatacgact atctaattcg tttttttgta aaatatttat ttttacacca gatttgatca
    10621 aataatccaa tgctttttgc cgctgtttgg tttctccagc taaagtaacg atcaaactcc
    10681 ctaacggcgt ttcttgtaat aactctacat tgccatataa aatattcgct gttacttgat
    10741 atttgctgta cagatgagcg attagaggtt cgttcgtctg atttccaata taacttaatt
    10801 ccaccagcca tttattggcg atctgatcgg caaaagcaga atgggcaatg atcgtttcta
    10861 atgcttggtc aagatgagta gctgttcgaa taaaatcttt ggtcaattct tcttcaggat
    10921 tgctgaagat ctggatgcta ctaccttttt caatgattct tccatcttcc ataaccgcta
    10981 ccttgttgca gatttctttg acaacttgca tctcatgagt gatcaggacg attgttaaac
    11041 ctaactggcg attcaatttt tttaacaatg caaggatctg aagggtcgtt ttcggatcaa
    11101 gtgcactggt tgcttcatca cataataata ccttaggttc attcgccaaa gctcgtgcga
    11161 ttgctactcg ttgtttttgt ccccccgaca gttgtttagg ataagcagat gctttttctt
    11221 ccaaaccgac aagttccagt agttcattca ctttttgacg acgttcctgt ttcgattttc
    11281 cagaatactt taaagaaaaa tcaacgttgt caaaaatagt tcggctttcc attagattga
    11341 aatgctgaaa gatcataccg atggcctttc tttcttttcg caattctttg ggagagagct
    11401 gactcaaatt cgtctgattg acaatgactt ctcctgctgt tggtttctgc agcaaattca
    11461 tcacacgaac taacgtactt ttccctgcac ctgagtaacc gacgattcca taaacatcgc
    11521 ctttttcaat cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga
    11581 aacatacaga aacatctcgc aattcaatca ttaaaattcc tccttatcta aaaaaagcgt
    11641 cctcaagaaa atgaaaatgt cattttcttg aggacgcttt tttgcgtgtt accacctcat
    11701 gttcaagctg tgttcacaca caactcctct tcaagtacga tcatactcta gcaagatatc
    11761 ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga attgctcgat ggccattttc
    11821 caatagtcgt tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc
    11881 gtacttacca tttcaatgca aataatattc aaaaaaaaat ccttttacta acataaacta
    11941 tgtcagtaaa aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa
    12001 gcccaacatc tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt
    12061 gcctacttat cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc
    12121 tcaccagcca gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga
    12181 tattttgttg ttgggattaa tctacatgag gaaaactaaa aagtcaagca cgaaattcaa
    12241 attatttttt ctatatttat ttttctgttt ttttatctaa catttcaata acagttcgaa
    12301 gatcttttcc ttcagcaaga agtttcaatt tttccttttc ttcgacggta taatttcgga
    12361 caaaactgga atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc
    12421 ctactaaaga atcttccact gcaaaggctg aactagcttc taatcctgtt cgttccaaag
    12481 ctgtcagata aggaatcggt gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg
    12541 tgtcaaagta atcagctaat ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag
    12601 tggtcgtagc aatgcctagt ttcatatgac gttcttttaa gtctgccaat atttcttttg
    12661 catatggttt taagtggatg gctccgcttg tcagctgttg ataaaaatat tcttcacgct
    12721 tcgctcggac ttcctgtact ttttcagggc tacctgctgc tttcactaaa atatcaaaac
    12781 tttgtttcca gttttttccg ccccaagcgt caacgatttc ttttgggata tggatatcaa
    12841 attgctgaaa tccccaaagc cagccatcac gatacgtttt ttctgtgtca actaacaaac
    12901 catctaaatc aaatattgca cctttcatct atacattaat cctttccaaa acaatacaca
    12961 tagtttagca cgactggtcc aaaaataaca gaagcaaaga accagccttc tcgtttataa
    13021 gagttcttgg aatcgatgga tatagatttt cttcacagct gtcacaagcg ccagataagc
    13081 gatgactgta gcaaataacc ataaccagta attgccagga agtggcatca agcctaattc
    13141 agcaccaaaa cttgtaaaag gaaggacaga accgacaata attcctaatg tagtgataac
    13201 aaacatggca aatgttacgt tgctttggat aaatggtact ttcggtgtac gcagtgcatg
    13261 gagcactaag gtttgtgtcc atagtgattc gacaaaccat cctgcatgga ataatgcgat
    13321 gaaaatcact tgctgtgaac tatccaatgt atggaaactt ccgtcaacta cagcaggaca
    13381 aataacaaaa tacatcaaca agtaagtagt aatatcaaat atcgaacttg ttgggccaaa
    13441 gtaaaccatg aatttcccaa tactagatgc attccatttt tttggctcgt ccaaatattc
    13501 tttatccatc cgatcccatg gcaaggaaat gcaggaaaca tcatagatta gattcaaaaa
    13561 cagaagttgt aaaggcagca ttggtaaaaa tggcaggaac atacttgcaa tcagcacgga
    13621 aaacatattg ccaaaattag aacttgctgt agccttgata tatttcatga tattcccaaa
    13681 agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat
    13741 cacgtctgca gattctttcg caatatccac agctgtatct acagaaatac cgatatcagc
    13801 cactttcatt gcaggagcgt cattgatacc atctcctaaa aatccgacag tatgaccatt
    13861 tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta cgaatacttc
    13921 atggtcttca gctacttgtg ctaattcatg atcactcatt tccgagattt tctcaccggt
    13981 aaccaattca ttgatctcta gacctacttg acggcacact gaacgtgtaa ctaattcgtt
    14041 gtccccagtc agaactttta cagctacacc atgttctttc agagctttca acgcttgttt
    14101 cgttgtttct tttggtggat caaggaaagt aagataacca atcaaaacca tgtctgactc
    14161 atctttgact gaaaattctc caacgacgct tggattggtc ttttgtgcga caccaatcac
    14221 tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt acggcttctt ttcgttcttt
    14281 tgttaatggt gtaacagtac cgccaagatc aataaattta gaaatgttca gcatttcttc
    14341 aatcgcgcct tttgtgatca tttgtgtttt accagctgga tcagcaacca caacactcat
    14401 acgacgacgt tcaaaatcaa aaggaatttc atccactttt tgataattga tcttatctgt
    14461 atctaaagtt tctgttgctt catcaatgat agccacatct aacaaatttt tcagccctgt
    14521 ttgatagtag ctatttagat acgcatggcg caaaacacga tcatcttcat ttccttcaca
    14581 atccatatga tattcaagaa tgattttatc ttgtgtcaat gtccccgttt tatccgtgca
    14641 taacacatcg attgcaccaa agttttggat ggcattcaaa tttttgatca ctgttccttt
    14701 tttggccatc acacttgctc ctttgaccaa attcgtcgta acgatcattg gaagcatttc
    14761 aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc aatccccttt
    14821 tgtaagccca ttgatgacga taactgctgg agccatcaat gacataaatt tgatcagtag
    14881 catggatgtt ttatggatgc ctaattcaaa gcttgattga attggttttt cagatagcgt
    14941 ttctgctaca tgaccaaata gtgtttcatt tccaacagaa acaatgattc cttccgcact
    15001 tccacttatg acattacttc ccataaagac aagattttcg taacttgttt cgctttgctg
    15061 atcgtcttca catactttcg catgcttttc tactggatag ctttcccctg tcaatgctgc
    15121 ttgggaaatg aataaatcct ttgcggacaa taaacgcata tccgctggta tcatatcccc
    15181 tgctgaaagc cgaacaagat ctccacagac gatttcttcc ataggaatct ctgtgtactt
    15241 tccattacgt tttacagctg ccgttacttt cacaagtgat ttcagttttt cagccgcttg
    15301 attggaacgt acggactgaa tcaacgtcat tgtcccgctg acgatgacca tagtgaagat
    15361 aatggctaca ccgaaaagat ctttatctcc aggagctgct aatacatatt ccgtgatgaa
    15421 tgaaataatt cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat
    15481 ttctacgatg aatggtgttt ttttgccata tgaaatcgca ttttcaccgt aattttctct
    15541 tgaacgttcg atttgttctt ctgtcaatcc atttgctgat gcgtggaata gttcaaataa
    15601 ttcttgtttc gtttttccag caaaagctgc atagattttt tgtgtatctt tctttttttc
    15661 catctcaatc tctcctttgt gtccaaaaga cagctgttct cgctgctttt ggtctttagc
    15721 tattcatcgg atcgatagag aaaaaactga aaggggctga gaacagatag ataatagcct
    15781 tggcctttcc tctagatcct tgtttttcga atgactgtca tccatttgtt ctcatctcct
    15841 ttactaagta attttaattc atgacttcca atcatttttc gaatagttat tttcccaatt
    15901 agaccacctc ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc
    15961 caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag atctcatcgt
    16021 tgagttttga cactgtacaa cgtaaaaagc agatacctgc ttttagctac cttatgaaaa
    16081 gccttatctc gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag
    16141 cctatgtttg tataggagcc tcacctaaca aatcttattc ggttttcctt cgttaatata
    16201 acaactaaaa aagaggatgt caaatcaatt ttccatgttt ttcatacttt ttttaaaata
    16261 cacacgttat attcctgtct cacgcggttt tatttgtctc attttccacc tcccgctata
    16321 atagaaatat agtaggaaac gaggtgctgt tatggaaaga acacttgtta ttatcaagcc
    16381 agacggcgtg agacgccatt tagtcggaag catcattcaa cgttttgaag caaagggact
    16441 ggccattgct gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag aacattatca
    16501 gcacttgact gagcgcagtt tttttgatga actgatcgat tacatgactt ccggtcctgt
    16561 cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggttggggc
    16621 aacaaaagct gtcgatgctg ttacaggtac gatccgagga gactacgcgc tgccggggac
    16681 tgaaaacatc attcacgctt ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt
    16741 tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta
    16801 aactgctggt ctagttgctg ggttcagcaa tggccagcag ttttttctct aaggacctcc
    16861 gttgattctt tcctaacata tattcgagca ctgtgaagaa ttcttggtag tctttcgtct
    16921 ttttttcccc taaagcagca gaaatcaatc tttgataata atctgccatc aatgtttcat
    16981 agatcactgt acttctttct ttatgtgcta agctccgttt catatcttca ttcacactac
    17041 gtgccttttt caaccgacca ttttctatat aaaacgaagt caggttcatt aaaaaagtaa
    17101 agagatactg ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt ttgattttca
    17161 aataattgtt ctcaatgaac cgatcactga atatccaagg aaaatcagct agaatagata
    17221 gttcaaattg accatagtct ggtatctgca tcaagtattc ttcaatgatt cggcattcat
    17281 aacgtaaaaa ttcaaattct gtctcagata cttctgtttt ttcgaccatg tgaagactca
    17341 aaaccaaata atacagatct tctgttttct taaaatgcct acggttattg ctgatctctt
    17401 tataaggatc aaagctgtct tcttgtaccc gatctagcat tcgtcttttt ttctttaact
    17461 ttattggttc atccagctgg tttcgcatcc attcatattc tgcaagtgtc atgttcattc
    17521 gctctaaaaa tgatctcaat gttacaaatg acattttttt cactgaattt gtctcaatcc
    17581 cgactagaga agtcttagaa cagattcctt ccgctaattg ggactgtgtc aacttcttgt
    17641 tgactcgaag tttcttgatc aattcaccat catacataat agttctcctt tttaatttaa
    17701 atttttccac attcaataaa tagatacgtt aaaaaggaaa aatacacgaa aaaaataaaa
    17761 aaatgttttt ttcataaaaa ataccaacct tcaagcatta gaatgttgtc taactactga
    17821 aggttggttt gattgtttat tgattatctt tttgctgttc aacgtctgtt cttgttactg
    17881 gatataagtt ggttgttgtt ttattgccat tttttgaaaa gacacttgtc gaatgttcgc
    17941 ctttttgctt ttcgatggct atgttacgtt ctgcttcatt tttatagtca tattttttcg
    18001 gatcgatcgt ttcaaatccg tttggcacat aaaaacgcaa gagattttct tgattcagct
    18061 tatctgaaag acttaaagca gatttgactt tttcctgatc tttggaaatt tcctcgtcga
    18121 ttcctgctgc tttcgtatcg atgatttctc ctgtttggtt attgtaagct tttcctccta
    18181 agatggtgta tttgggtgtc acgaagttcc catttcgaaa agccactact tgatcatgct
    18241 gaggagataa taaatccgta ccaaaatgga gatagtcttt atcatcgatt cccaagagat
    18301 gaagtaatgt cggcaaaaca tcgatttctc caccgtattc gtgattgact gaccctttag
    18361 tataaccagg tatatgaatc attaatggca cacgctgcat ttgtgcgttg tcaaattcat
    18421 tccatgtatc tgggtctttt ccaagagcac ttgctaagtc tttattatct gtatttgaga
    18481 tgccaaaatg atccccataa atcacaaaga tcgaattttg gtaaatagcg gattttttca
    18541 ggtacgtaaa aaattgttcc aatgactgat ccaaataatg tgccgttcga acatagttat
    18601 ctactgtgct atttccagtg ttcaaagatg gaaaatcgaa attcttatca tccgtataat
    18661 aaggcgtgtg attggttacg gaaaggaatt tcgtgtagaa cggctgttgt agatgttcaa
    18721 gatattgggc agattcactg aataaatctt tatctaatat tccataacct agcgtttcat
    18781 ctgattcatc aaaataagat cgatcaaaga aattgtcata acctaagttt ttatatacat
    18841 gatctctatt ccaaaaacta gctacattcc catggaaaac agcgcttgta tagccttgct
    18901 tttgtccaag gatcgctggt gcggcttgaa agacattatc tgacccaagt tctgtgaata
    18961 atgagccttg aggtaaacca aatgtgccag tttccaacat attttcagca tcacttgtct
    19021 ttccctgtcc tacttcatga aaaaaattat caaagctgat cgttgcttga ttctggaaaa
    19081 tactatttaa aaacggtgtg acttcttgtc cgtctacctt catattaatc aaaaactgct
    19141 ggaaactttc taaatggaga acgatcacat ttttcccttt agcgatccca aatgtttcgg
    19201 gatttggctc tgcataatgt ttctttgtat aatctaaaac ttcatcaatc ccattactgc
    19261 ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat tccatcataa atagtatacg
    19321 catcgattcc tagatatttg acaatatatg aacggtcaaa tgttcgctgc agcaattgtg
    19381 gacggttggc ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa agacaggtca
    19441 cagcgatagc catcggtttg ctgacaggtt gacttttgat aggtattttc ttgatcgtca
    19501 accaaaccag tatcccaatg aacgtagcaa tatccagcca gtagaggaca tcatggggtt
    19561 tcatcaaaga aaaggagctt cctgataacc cttgcgacac tttcgaaaat cccaatacag
    19621 atttgactgt aatgaaatct gtgaattccc gataaaaaat aatgttcaag tacaacaaaa
    19681 gcgtgtttaa aatatcaatg atcaaaagaa tggctaatgc tggctttggc tttttaatat
    19741 acaaagaaag gccaaaaaac aataaggtag ttgcaatcgg gttgatccac aagataaagt
    19801 actggatcgt accttccaca cctaacgaaa aatctgcata gtaagcaata atcgtcttca
    19861 tccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg attttggcac aattttttaa
    19921 gttgtgtcac gtatatcact tctctctatt cacatttttc cttattttgc tcctgaaaaa
    19981 tgaaaattcc ctaaaaaagt tgttatctta agattttttt attctttcta aagattattc
    20041 cgtgcacttt tcacagcata aaaccgctgt gatataagcg attgaaacag taaggtgata
    20101 aaagtcttgt cgcagctcct gtttagttga tgcaggacag ctttttcaca acttcttctt
    20161 taaacggtgt tctcctgcca actcctcggc cacaagtcac aatatttata aaacatgaga
    20221 agctgttttt tgaaattgtt ccttgtggct cagagttaaa cggcaagctc aagacctctt
    20281 gata
//
LOCUS       DACGLH010000046        20263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.049, whole genome shotgun sequence.
ACCESSION   DACGLH010000046 DACGLH010000000
VERSION     DACGLH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.049"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            208..2250
                     /locus_tag="IXT21_001839"
     CDS             208..2250
                     /locus_tag="IXT21_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4869226.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="IXT21_001840"
     CDS             2261..2881
                     /locus_tag="IXT21_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ4869227.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="IXT21_001841"
     CDS             2893..3351
                     /locus_tag="IXT21_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4869228.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="IXT21_001842"
     CDS             3370..3648
                     /locus_tag="IXT21_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4869229.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="IXT21_001843"
     CDS             3673..5040
                     /locus_tag="IXT21_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ4869230.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="IXT21_001844"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="IXT21_001844"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ4869231.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="IXT21_001845"
     CDS             6227..6718
                     /locus_tag="IXT21_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869232.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="IXT21_001846"
     CDS             7161..8360
                     /locus_tag="IXT21_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4869233.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8855..9613)
                     /locus_tag="IXT21_001847"
     CDS             complement(8855..9613)
                     /locus_tag="IXT21_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4869234.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9781..10716
                     /gene="pfkB"
                     /locus_tag="IXT21_001848"
     CDS             9781..10716
                     /gene="pfkB"
                     /locus_tag="IXT21_001848"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ4869235.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10713..12158
                     /locus_tag="IXT21_001849"
     CDS             10713..12158
                     /locus_tag="IXT21_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4869236.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12186..12641
                     /locus_tag="IXT21_001850"
     CDS             12186..12641
                     /locus_tag="IXT21_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4869237.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12660..13634
                     /gene="lacD"
                     /locus_tag="IXT21_001851"
     CDS             12660..13634
                     /gene="lacD"
                     /locus_tag="IXT21_001851"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ4869238.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13745..14306)
                     /locus_tag="IXT21_001852"
                     /pseudo
     CDS             complement(13745..14306)
                     /locus_tag="IXT21_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14450..15064
                     /locus_tag="IXT21_001853"
     CDS             14450..15064
                     /locus_tag="IXT21_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869239.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15305..15688
                     /locus_tag="IXT21_001854"
     CDS             15305..15688
                     /locus_tag="IXT21_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4869240.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16051..16560
                     /locus_tag="IXT21_001855"
     CDS             16051..16560
                     /locus_tag="IXT21_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ4869241.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16660..17310
                     /locus_tag="IXT21_001856"
     CDS             16660..17310
                     /locus_tag="IXT21_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869242.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17761..18699
                     /locus_tag="IXT21_001857"
     CDS             17761..18699
                     /locus_tag="IXT21_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ4869243.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18712..19764
                     /locus_tag="IXT21_001858"
     CDS             18712..19764
                     /locus_tag="IXT21_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4869244.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19822..>20263
                     /locus_tag="IXT21_001859"
     CDS             19822..>20263
                     /locus_tag="IXT21_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ4869245.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
BASE COUNT         6421 a         3221 c         4277 g         6344 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttagacg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacattctt attattatat atttgcgtaa gtagtcctgg
     8701 tagtctcaat gcactattct atcttgaaac taagaagtta tagaaggagc aatgatagaa
     8761 agcacgagaa tacaaatagc gattaaatat ttttctagac aaccttagtt aattcacaca
     8821 gatatatttt aggtacagtc ttacagatat agagttagtg ctgtgtgaat cctgatgttc
     8881 gcagtgcata ggagtcaagt aagattctag ccagtataga aagtggaagt ctggagcgta
     8941 cctcataatc tgggaagaag gagccttctg atttaaagcc aacaaaagga atctcgatat
     9001 gttccatcaa acttgaatga cgattagcag taagcaaaat tgtcccgatc tcagctttat
     9061 aacagttttc agctgcggta gtcaattcct ttgtttcacc gtttagtgaa acaaagatca
     9121 cgatgtcatc tttggataat tttctactaa tgttttttat gatattggga tcagtatgca
     9181 tctcacaata tttaccggta agttgaaatt tgaccatcat ttcctgagca atcaattctg
     9241 aaaaacctcg ggcaaaaatc atgattcttc tagcagcctt gattcgttga atagcatctt
     9301 caatattccc gctttctacc atattgatgg tgcggattac ctcttgctca tttttaagaa
     9361 tagcagtctt tattccttca tcaactttat caagagcggc aaaattgata tttgtatttt
     9421 ctttttcttt taacgaatgt ttaaaagacg taaacccctc atatcctttt ttcttcatag
     9481 tacgaacgac ggttgctgtt gaaacgttgg catattcact tagttttaca atggaatagg
     9541 aggggatttc gagtatgtga ctttgaataa aatcccagag atatttctcc gattcactta
     9601 attttgtatc catattttgc ctccgattag ttaggataat tctattgtaa agcgggagtt
     9661 tcctttactt caagtttatt taatatattt tgtaaaagtt ttcaatgaat ttagtcattt
     9721 atgaaaacat ttacattcga tcttgataca taatgaatat gtaagaaagg aggagcgtaa
     9781 atgtcagttt atacatgtac tttaaacttg gcaatcgacc tttttatcga aacagatgaa
     9841 atgaaaccat cccaagtcaa tcgaacaaac gatgatgata tacaagctaa tggaaaaggt
     9901 gtgaatgttt cattgatcat gaagcaatta ggtatcgata gtacagcttt aggatttaaa
     9961 gcaggtttta ccgggaatta tattgaggat tatttgcatc aaaaagatat tagcactgat
    10021 tttattagcg taccgggaat gactagaatc aacgttttta cacaagttaa taaaaccaat
    10081 gaagaattca aattggttaa taaaggacct gagattcctc aggaacaaat caaaactttt
    10141 ctaaatcaaa tacaaaagct gcaagctgac gattatttgt gtgtttctgg cagtttgccg
    10201 cgaggggtct cacccgaaat tcttattgat atcagtcgta tttgttttga aaataaggtc
    10261 catctgatta ttgattcaag ctatccggaa ataattgatt gtctgccgta tcagccgttc
    10321 ttattaaaac caaacgagga ggaattgcaa tcttggtata atgtcgagct tagtgatcgt
    10381 gaaggatata ttcattatgc tgaacgcttg attgccgatg gagcgaaaaa tgttctcctt
    10441 tctctaggag gagagggagg aatattttta aataaggata tcgtttattc tgggaattct
    10501 cctaaaggta aagtagtcaa tacagcgtgt gctggtgaca ctttgttggg aacatttctt
    10561 gcaggatatt taaaaggtga acaggtagat attaatttga agaaaagttt agcagctgga
    10621 agttcaaccg cttttcgtaa aggattaaca gatttttcgg atgttgcaga gttggaaaaa
    10681 caaatcgaaa tcaaagaggg agcgagtaaa aaatgacaac ttacaaaatt gttgccgcaa
    10741 caggatgtcc aacaggaatt gcccacactt atatggctca agaagcattg gagcaggcag
    10801 ctaagcgcaa aggggtatct atcaaagttg aaacccatgg tcaagtaggg atagagaatg
    10861 aattaacacc agaagaaata aaaaacgctg acgctgtaat cattgcagct gacaaagatg
    10921 ttcaagcaga acgtttcgca ggtaagcgtg taatcgatgt gcctgtcagc aaggggatta
    10981 aagatgctga ccaattgatc gaagatgcgt tggctggtaa aggttcaatc actgcagaaa
    11041 acaaaacagt tgacgctgct ggtgagaaag cggagatggg ggttgcagca ggcattggtc
    11101 acagtattta taaaaatctc atgaacggtg tttctcatat gctgccgttt gtagtggcag
    11161 gtggggtaat tattgcaatt tcgtttgcca tctggggtgt gtactctttt gatcccaatg
    11221 atcctactta caatccgact gccgctatgt taaagggtgt tggtgatgcg gcaatgggaa
    11281 tgatggttcc tattttagca gcttatatcg ctgaaggaat cgctaaacgt cctggtttgg
    11341 ttgttgggtt tgttggcgga ctgttagcca attctggcgg agccggtttc ttaggcggaa
    11401 tttttgcagg tttcttagca ggctacttta tcttactatt gcaaaaggtc atgaaaggct
    11461 tgcctaaaca attggatggt ctgaaggcga tcttcttata tccggtgatc ggtgtcttgt
    11521 ttgttggtgt agtcatgtct ttattagcaa atccaatgga agcagtcaac caaggaatga
    11581 tgaatttctt ggcaggattc caaaattcta atccattaat tttagggatt atcgttggct
    11641 gtatgtcagc atttgatatg ggaggtccag tcaataaagc ggcttatgtt acagggactg
    11701 cgttgttggc acaaggtaac catacattta tggcaggggt ttcggcagct tgtatcgcgc
    11761 ccccattgat tactggattt gccgcactgt tttttggaaa atatttcgac acgaatgatc
    11821 gcaacgctgg tttagtcaac tttatcttgg gctcaacgca cattaccgaa ggagcaattc
    11881 catttgcggc taaagatccg ctgcgtgttc tgccaactat gatgctaggt tcatcaattg
    11941 ctgctgtact gacatatatg tttggtgtcc aagttcccgc acctcatgga ggttttctag
    12001 tattacctgt tgttactggt aaggttgcat gggtagggtc gattttactt ggttcaatcg
    12061 ttggcggagt aattttaggg ttgatccaaa aacgtagtgt agaaaaacgt gaagcagaag
    12121 cagctgctgt tgaaatgaaa gaagcaacca acaactaaag gttattctag aaagaaggat
    12181 ttcaaatgac tttgatcaaa acaaataaag tttttatgaa taaaaatttt acgacgcaag
    12241 atgaggtaat gaacttcctt gcagatcagt caattgtctt gagcattagt gaagaccgag
    12301 atgcagttta cgaaaaattg atggaaagag aagaagaaag cacgactggt atgatggatg
    12361 gttttgccat ccctcatgcc aaagatgcta gtatcaagga tgccgatatt gtgattgttc
    12421 ggttaaacgg taaggtcgat tggaaaagtt tagatggaga aggaacggat tttgtaatcg
    12481 ctctgtttat tccagattca gaggctggaa caacccatct aaaattttta tctcaggtag
    12541 ccagactttt gatgcacaaa gatgtgacag aaggtctgaa acaagcccag tctgctgaag
    12601 aaatagctga acttttaaac caaaaattag aagaaaacta aaaaggaagt agtgaaatca
    12661 tgcctttaac agaaaaaaag aaaacttata ttcgtcaact aagtaatgaa agtggcatca
    12721 tcagtgcgtt agcaatcgat caacgtggtg ccttgaaaaa aatgatcggt aaatttcaaa
    12781 ataaatcagc aaccgatgaa caaatcatca atttcaaaaa aatcatttct tctgaattga
    12841 ctccatatgc ttcagcaatc ttattagatc cagaatatgg gttgccggca gccgaagttc
    12901 gcgataaaaa tgctggtctg ttgttggcct atgaaaaaac tggttacgat gcttcaactc
    12961 caggacgttt accggatatt ttatctattt ggtctgtaaa acgtctaaag gagcaagggg
    13021 ccgatgcttg taagttcttg ttatactacg atgtagatga atctgatgac atcaatgacc
    13081 aaaagaaggc gtttatggag cggattggtt ctgaatgtgt tgctgaagat ttgccatttt
    13141 tcttagaatt ggtttcttat gatgccaacg atcctgatac ttcttcgaag gaatatgcct
    13201 tgaaaaaacc gcataaagta aatgaaatga tgaaggaatt ttctaagcct cgctatggag
    13261 ttgacgtttt gaaagtggaa gttcctgtta atatgaacta tgtggatggt tacactaaaa
    13321 atgaagttgc ttataccaaa gaagaagctg ctgcttattt caaagaacaa tccgagacta
    13381 ccaacctgcc atttattttc ttgagcgcag gagtaactgc tgaattgttc caagaaacct
    13441 tgaaatttgc aaaagaggct ggctctacat tcaatggggt actctgtggg cgcgcaactt
    13501 gggcgaatgg tgtagaacca ttcgttacta aaggtgaata tgctgccaaa gaatggatgc
    13561 agacacaagg acgcaagaac atcgaagagc tgaataatgt tttgaaagaa acagcaacag
    13621 ctattaaatt gtaagaaagc tttaactata accttaagtt tgtaacctaa tgatttttaa
    13681 taatcaattt gagcttttaa ataaagaatt ggaagcgtcc cgacgcttcc tttttcgttt
    13741 gtttttaagg catgataggt tctatgttgt cgttcaataa gcgcaagaag gtcagcattt
    13801 ttttcttaga gtatgcaaaa ttgcttttct acccaatgat ttaaaacgga gaggcaccct
    13861 tggatatggt agatggtgaa gtaacgcatt tcttctgtga tcacggtttt tttgtcttct
    13921 tgtagccaga tttttaaata attttcttcg atcagattat ttaatctctt ttgaaattgg
    13981 atatctccgt ggttgatgac gaataacttg tatacgaacg tattcgtata gacatagtcg
    14041 atcaattgag cgtatagatc ctcgtagcta tgtgtcgtat cgtaggaaat gatttcatcc
    14101 aagtcttgga aaaagctatt ttccagtttt tcataaagat catagatatc cgtataatgt
    14161 gtataaaatg tcgaacggtg aagatcagca gtttcggtca gttcacggat ggtgatgttt
    14221 tgaatttttt tatctactaa taattttgat aatgcttctg ctaacgcttt ttctgttttt
    14281 ttgacgcggc gatcctcttt tttcattttg cagcctccta atcaacataa tcaaggaatt
    14341 gtgtcggtta tccgacaatt tcaccgctat ctgaacatag ataaccgaca ccttgtctat
    14401 tataatataa gtatagcata tcaatattta aagaatagga gaataagtga tggaattagc
    14461 ctttaagcaa ctggaaaaga aagactacaa aaaagccatt caattcgcga ttaaaggtat
    14521 gcactttgat tggtatacca agaacaaact ccttttgaat ctttatggaa gatacttttg
    14581 gtatctagaa ttgaatcgcg cgacacaaat cattgcttta tatgcggatg atgagttggc
    14641 aggtgtttta ttagcagaca tcaaaggaaa atcaaaaaag catcattcat ttagtcaaaa
    14701 gttatatgtt aaactatttg atttcttaca aaatgcattt gtgaaagaca gcaaaggcgg
    14761 gtatgacgac accaatgacg aattgctttc agactattta aaaaaacatt ccccagatgg
    14821 agaaattgtc tttttagctg ccaaccctga tttaaagatc aaggggatcg gtagtaaatt
    14881 attaaaagaa tttgaacgcc gagagcaagg aaaagaagta tttctgttca cggatagcgg
    14941 ctgtacgtat caattttatg aacgacgcgg tttcgaacgg aaaaaagaaa aagataacgt
    15001 catgaagatc cttaataaag aagtagatct ccgctgtatg ttatatagta agatcattca
    15061 gtaatgtttt ttactatcag aaagcagaag taccacgaag gtcaatggaa aaagcgagta
    15121 attgatccat ttagatgaag ataggtgtag tctcaaagat attagctggc aaagatatcc
    15181 ccagttaaaa atagatttct gtatccaaca ataaaaatat ttttgtattt attagtatat
    15241 atactttaca aaaatttgta tatatactaa tttttttgta ggatcaatga aatgagggga
    15301 aaatatgcca aaatataaag aaatagcctt ggctctaaaa gagaggatcg ttgagggaaa
    15361 atatcaggaa ggttctaaat tacctgagca agaaagtctg gctagagagt ttaaaacaag
    15421 ccgtgttact atccgcaaag ctatacaact ccttattgat gaaggtttgt tatatacgag
    15481 acgtggatct ggtactttta tacgaagcaa tatcagacag gacaatgaga atgtcactca
    15541 agtaaataat gtatttggca cttcctcaca agaaaataca aatgtgacaa gtaaaataat
    15601 ccgttttgat gtccggtttc cgacagatta tgaaaggcgg ctttctgcaa cctacttccg
    15661 atccccttcc tgcaaatcat gggtataaaa aataggcata ttaagcgggc taggaggaga
    15721 aatattcaca tatcattttt tcattccgca agctgcgtca agttatttgg aatttgtaga
    15781 acagatgaga gaggtagaag cagcactaca aactactttt caataagtag gaattataga
    15841 aattttactc aaaaaatctt cattaaaaaa tctgactaaa taagttgaga aaacagaata
    15901 tacttcaata tccagtgatt tataaatatc attcactcat gatacccagc tccgtatcat
    15961 aagtgatgcg gtttttatca ttgatcctaa aaatcttagc aagacaaaaa gtacgtaaga
    16021 aagtaaagac taaatacttc ggaggaacaa atgaataaca atgataggtt attgcgattg
    16081 cgttatgcga ttgatttgaa agatacggat ttgattaaag cttttgagct aggcggtgta
    16141 ccactgacta aagaagaagc tcaggcggta ctgaccaaag tacaggataa gaacaaagat
    16201 aatgcagaaa ataatgtgta tgaaaaaacc atcaacaacc aagtttttga cgcattccta
    16261 aatggcctga ttctcttggc gcgtggtccg caaaaagata agccgatagt agacacgccg
    16321 caaaaaagca aggaaatcaa atatatcaac aatgtgttgc taaaaaaatt aaaaatcgcc
    16381 ttgtctatga ccactgatga tattttagaa gtttttgctg aggcagaact ctatccatca
    16441 aaaggcgaaa tcggggcatt tttgagaaaa gaaggccaac gaaattttaa gccttgcgga
    16501 gataaatata tgcgtaattt tctcaagggg ttaggaattt ataatcggag aaaagtataa
    16561 agaaatttca tgagaaatca aaacaatggt tgttttgatg agagctagtt tttgtatgag
    16621 attaaaatat tatcttaatc gaggagctgg attggtttta tgaatgatga atataagaat
    16681 gatgaagata agatgctttt tgaagagata gagaatcgat gtaggctcaa ttttgagctt
    16741 cggggaaaga tgtctttaat acaacaaaaa aagtatttag cgaataaatc agagtttact
    16801 ctgggacatg ttgaaaaatt aatctcggat tggattagta gtagaagcga atttacaaaa
    16861 ataaaacagc cgattaaatt tgacatgaaa aaacttttgt taaataagag tgagataggt
    16921 aatagagatc aatatattag agctaaaggg caagaaataa ttgattcatt aggtgaagtg
    16981 aggtcataca attatttgta tgtaacacat agagctgatg gaatggtcat tactgtgggg
    17041 aaatcttctt ctaatgatat ttttttagat ggagatcttt tttatcaact caatacaaat
    17101 cacttgtctg gcactgaaaa tataatttta aggacggaat atggcaatga aatatttgca
    17161 aaatatgatg agattttaaa aaattatttg gactgggctt ggataattcc ggttgagtca
    17221 ggagatgcta agaaactgga gagattattg ggcgatgaac taattaataa aaaagtacca
    17281 attttaaatt attattccca tagacaataa aaggctattt ttcatgataa tactagttat
    17341 aactgtaaaa atggaagaaa atctgggaaa gaagttttta aataagtgta ttgttcgatt
    17401 ttaataaata ataagcaata ccatccgtct gtgttgcgca acatcgtact acaatgaata
    17461 ttttttcaca aaaagaaaat catccatcga atgaatggaa ggttttagta tacacgaggt
    17521 taatgtcccc tcttttataa tcagaggagt gaggtttttt ctatgaatgt ttctattttc
    17581 ttattgctag tctagtttct cttacgatga aattgggatt ctattccagt ttttattgat
    17641 ttgctgataa aataacttga atggtggata taaacatgat aaataagaga agaataacaa
    17701 aacaaggttg tgaaatgatt ctcaaataag tataatagaa agaaaaagga ggaagaatca
    17761 atgaaaatag caattgcagg cgctggcgca atgggaagtc gctttgggtt aatgcttcac
    17821 caaagtggga acgaagtatt attaatcgat ggctgggcag aacatgtcca acaaatcaaa
    17881 gaacatggat tacaagcaaa ttttaatggt aaagaggtag aagcaaaact accaatcgtt
    17941 cttcaatccg aagtagaaaa agaagatcaa gttgatctga ttattctatt taccaaagcg
    18001 atgcagctgg aaaaaatgct gcaggatatc caatcattaa tcaaaaaaga tacagaggtc
    18061 ttatgtctat taaatggtat cgggcatgaa gatattattg agaaatttgt accaatggaa
    18121 aatatctata ttggaaatac catgtggacg gcaggtcttg aaggtcctgg tcaggtcaaa
    18181 ttatttggaa gtggttcagt agaattacaa aatttaggtg atggaaaaga agcagctgca
    18241 aaaaaattag cagacaaatt gtctgaatca ggattaaatg ctcatttttc tgacaatatt
    18301 cactattcta tttatcgcaa agcatgtgtg aacggaacaa tgaacggact atgcacaatc
    18361 ttagacgtta atatggctga gctaggaaaa acatcgactg ctcataaaat ggtggcgacg
    18421 attgtcaatg agtttgccaa agtagcagca gtagagaaga ttgaactaga tgtcccagaa
    18481 gtcattgcac attgtgaatc ttgttttgac ccggaaacaa ttgggttaca ttatccttca
    18541 atgtatcaag acttgattaa gaaccatcga ttaacagaaa tcgattatat caatggcgca
    18601 atttctagaa aagggaagaa atatggtgtt gcgacgcctt attgtgattt cttgactgag
    18661 cttgtccatg caaaagaaga tagtttgaac gtaaaataaa ggagagattg aatggaaact
    18721 tcacaaaaaa tatcaccgaa aatttttctg aataaagtcc tcgctggtac agcaacagga
    18781 atcattattg gattgattcc taatgcagta ttaggtgcta ttttaaaata ttttagtgcg
    18841 tatccctttg cggtaacgat cgcacagtta gcagttattt ttcaattagc aacacctttg
    18901 atcattggtg gtttgattgc cttacaattt ggatttgatc cgatgagaat gatggttgta
    18961 gccggagctt cttttgttgg ttctggtgta gtcaaattca cacctgattt gggtgaaacg
    19021 ggtgtttacg ttggtgctgg tacaggcgat attattaata cgatgctgac agcatcgatt
    19081 gctgtcgggc tgattttact gattggaaat agttttgggt cagtgtcgat tgtgttaaca
    19141 ccgattgttg taggtgtagg tgcaggttta cttggttttt atttattccc ttttgtaaca
    19201 gcaattactt ctgcaattgg atcattgatt aataacttca ctaccttaca accgctgtta
    19261 atgtctatct tgattggttg ttcctttgcc tttttgatta tctctcctat ttcaacagta
    19321 gcaatcggaa tggcaatcca actagatggt gtttctgctg gtgcggctgc gatgggcgta
    19381 gctgcaacca cttttgtttt agtagtgaac tcttggaagg ttaataagtc aggtgtgaca
    19441 attgcgattg ctttaggcgc tatgaagatg atgatgccta atctatttag aaaacctgtg
    19501 attcttattc cttgtttagt tagtgcagtg attacagcta ttcctgtcgc tctattttct
    19561 atttcaggta cgcctgcttc tgctggtttc ggtgtggtcg gacttgttgg tccgttggct
    19621 tctttagatg cagggctaaa tggctttctt gttgttcttt gttggttagt catccctgtt
    19681 tttgcggcac taattactca atttttatgc gagaaagtat ttaaactgta tgatcgagaa
    19741 gaagtcttca aatttttagg ctagtagatt ttactaagaa attttcaatc aatggatgcg
    19801 taagtgagat gtcaacaaca tctgacttac gtttttttgc caaaatatgg tttttatttt
    19861 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
    19921 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
    19981 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
    20041 ttgtatcacc tatttgttgg aacatcaaaa acacttgcaa tacttgctgt tgcaacagtt
    20101 ggtttatatg ggataggtga ttgtattttt actgggttat tcagtatcaa tacgaatgaa
    20161 agctcttgga atctatcgac ttggatccat aatacaggtt ctgggctagg ctatgcaggc
    20221 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaa
//
LOCUS       DACGLH010000047        19998 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.051, whole genome shotgun sequence.
ACCESSION   DACGLH010000047 DACGLH010000000
VERSION     DACGLH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.051"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(190..1557)
                     /gene="nhaC"
                     /locus_tag="IXT21_001860"
     CDS             complement(190..1557)
                     /gene="nhaC"
                     /locus_tag="IXT21_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ4869246.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1666..3087)
                     /locus_tag="IXT21_001861"
     CDS             complement(1666..3087)
                     /locus_tag="IXT21_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4869247.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3330..5207)
                     /gene="tdc"
                     /locus_tag="IXT21_001862"
     CDS             complement(3330..5207)
                     /gene="tdc"
                     /locus_tag="IXT21_001862"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ4869248.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5498..6754)
                     /locus_tag="IXT21_001863"
     CDS             complement(5498..6754)
                     /locus_tag="IXT21_001863"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ4869249.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7334..8635
                     /locus_tag="IXT21_001864"
     CDS             7334..8635
                     /locus_tag="IXT21_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ4869250.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8704..9303)
                     /locus_tag="IXT21_001865"
     CDS             complement(8704..9303)
                     /locus_tag="IXT21_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4869251.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9557..10438
                     /locus_tag="IXT21_001866"
     CDS             9557..10438
                     /locus_tag="IXT21_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869252.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10451..12061
                     /locus_tag="IXT21_001867"
     CDS             10451..12061
                     /locus_tag="IXT21_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ4869253.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12138..12797)
                     /locus_tag="IXT21_001868"
     CDS             complement(12138..12797)
                     /locus_tag="IXT21_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ4869254.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13080..13745)
                     /locus_tag="IXT21_001869"
     CDS             complement(13080..13745)
                     /locus_tag="IXT21_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869255.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13892..14497)
                     /gene="dhaL"
                     /locus_tag="IXT21_001870"
     CDS             complement(13892..14497)
                     /gene="dhaL"
                     /locus_tag="IXT21_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ4869256.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14525..15508)
                     /gene="dhaK"
                     /locus_tag="IXT21_001871"
     CDS             complement(14525..15508)
                     /gene="dhaK"
                     /locus_tag="IXT21_001871"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ4869257.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15510..15905)
                     /gene="dhaM"
                     /locus_tag="IXT21_001872"
     CDS             complement(15510..15905)
                     /gene="dhaM"
                     /locus_tag="IXT21_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ4869258.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(15995..16954)
                     /locus_tag="IXT21_001873"
     CDS             complement(15995..16954)
                     /locus_tag="IXT21_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4869259.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            17124..17444
                     /locus_tag="IXT21_001874"
     CDS             17124..17444
                     /locus_tag="IXT21_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ4869260.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(17556..18425)
                     /locus_tag="IXT21_001875"
     CDS             complement(17556..18425)
                     /locus_tag="IXT21_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4869261.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(18521..19825)
                     /locus_tag="IXT21_001876"
     CDS             complement(18521..19825)
                     /locus_tag="IXT21_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4869262.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         5702 a         4134 c         3653 g         6509 t
ORIGIN      
        1 aaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
       61 acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttaaaaag
      121 caattttctt gagtttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
      181 ttccttcctt caatttttta cacgtcgacg attcaataaa aaaccgccta atattgtaaa
      241 gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat
      301 cgttcccata atgaatgtgc cgctgactcc ccacggtacg agagaattga cggtcgcacc
      361 tgaatccgct agtgttcgtg tcaaataggt ttttgataag ccaacgtcat caaaagaacg
      421 cttgaatgtt tctcctggaa gaatgatgga cagatactgt tctccaacga tcagattgat
      481 tccgatacag ctcaaggcag taaacccgac taaacgagaa ggtcggtcca tcttttcttt
      541 taattcttga acgatggtat caacgatcag atatttgatc agtaacccac ctaatgccaa
      601 tgcaagtaaa atcaaactag cagaacctaa catactcatg atcccgcctc gagaaagcag
      661 ttcatctagc tttggatcag gtgtatttgc gacataacca ttcatcaaga tgtctgctgc
      721 ttttccgacc gtcaaacttt tctcatggat aaaagaaaga acgagagcag ctgtcgatcc
      781 cgctagtaat gtagggatag ccggaatttt tttgattgcc cccccaaata aaataactag
      841 tggaatcaat gtccaaggag agatccaaaa gctcgctttc atgttttgga tcatttcatt
      901 gatactagct agatcggctg aatgactgtt atgtcctaat atcgtataaa aaatcaacga
      961 aaggacgaat gctgggaaat ccgtataacg cataccagcg atatgttcga atagattaac
     1021 tcctccaata cccgctgcca agttggtcgt atctgataaa ggcgagcagt tattccctaa
     1081 aaaggcaccg gaaacgactg cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc
     1141 gatccccaaa aaagcaattc caatcgtcga aatcgttgta aatgaactgc cgacagtcac
     1201 tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt
     1261 aaatccataa accataatcg tagggatggt tccagataaa atccatgaag caactaagac
     1321 accaatcaaa agaaaaatca ggattggaat gatccctgga gtgattcctt tgatgatccc
     1381 gtcatggatc tcatcccatg tgaacttttt aaatctgcca taaaacaaca aacacatgaa
     1441 agctgcaagg atcgggatat gtggcgagag ctggaatccg atgatcagta ctcctaggag
     1501 tgccaacaac acgatcaata cagcgatact ttcttttaaa gaaatatttt tattcatcgt
     1561 cgatctccta tttcaaatga aaaggccctg ccatgaaaaa actattcact tggcaggacc
     1621 tcctactaaa tcagcgacaa aacgtcggct gaaagactta tttttttatt tttcttctaa
     1681 acttttttct acctcaatcc cccaagaatc tttcttgaag tgattgatga tcaatggaat
     1741 aacgaacatg accactgtga tagctgctac taataatgca tcactcaatg tcgctgtacc
     1801 taatgtagca aatacgacaa cgatgataga gaagatcagc atgcagctga ctacccaagc
     1861 taatccgttt ccgtttttac caattcggta aggacgttcg aattctggat gtttcttacg
     1921 cattttaagc aatgaaacag caatcaaaat gtacacgata caataaataa cagttgtagc
     1981 atttgtaagt gtcaagaata cagcacttac gtcatccatt acaccataaa gcaaagcaaa
     2041 taatgaaata acgattgatt gtgttagaac gacatttcta gatacgccgt atttgttttc
     2101 tttatggaat ccgaattttg gtggaaggaa tccttcacga gctacttgga tgattgtttt
     2161 tgatggtcca gtaacccaag ctgataattg taaaagcaca ccaataaata ccatgaagct
     2221 gaagatattt ccgatcactt ctggtaagcc taggatttga cagtcaatca agataggctg
     2281 cgtgatattt gctaattcca tttttccatc tggtacgaca tttgctacaa gcatcgcatt
     2341 gatgacattc aataaaacta atccgatcaa agcgataaat acgccttttg tataattttt
     2401 tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt tcgatcccaa caaaaataaa
     2461 tgtgatacca gctaagtatt tcaatgaatc aatatgttcc aagtcaggga aagcttttga
     2521 ccatgaaaag tcacctagat agccgtttga gactagtccg actttgaagg ctgccaaaac
     2581 acctaaaacg aacatgatga ctactgggat atatacccct agccatacac cgatgttccc
     2641 gccgactttt gccatatcga atttcaagtt caagatcgtg atgatccagt agataactaa
     2701 aatacaacct aaaacgaacc agtgattatt gccaagttct acattaccaa atgtgtttcc
     2761 taacaatggt cctaaagtag aagcgaccat taccatacct gggaacattt gcacccataa
     2821 tagccatgca acgacaaatc cccatttgct gcccagtgct gtttttaccc ataactgagg
     2881 accgccttct tgaggaagca tcgtggaaag ctcaccggaa accattgata tcggaccggc
     2941 aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc caaccgactg cggctaacgt
     3001 aggaatactc cgaaccgttg cacacagtgc cattgtcaga ccgataaagg tcccaagacc
     3061 cattttactt acttcagatt tactcaccgt tgacactctc cttgattttt tggataaaat
     3121 aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa ttcagcgaat atttgagggg
     3181 attctttgag tttattgctt gaagggtttc ttcaaagaca acctcaaaac aaagggatcg
     3241 aagagcttag gtctcccctt gctcttcaga tccaaacatg cgtcagaaac agttagtatt
     3301 caagctgttt tctaacatga ttaccttgct tattttgctt cgcttgccaa taattggtct
     3361 gcgtagattt tttccaattt ttcttgaaga gctgctttga ttttttcagc atattcttca
     3421 aagtgttctt ctttgttcat gtatggtgtc attacgctgg cacgcaatac agttactttt
     3481 ccggcacggt tccattcttc atctgagaat cctagttggt tcacaaattg taatgggctg
     3541 ttgccatagt ctgggatagc aaagtcagta tgtgaagtta agaactcatt accgtaaatg
     3601 cttcctttca cgtatgaaga ataatcatat acgtcatggt tcaatttatt cattgcaact
     3661 aagtcatcgt tgcctttttc tttgaataca tagtctacca tattgaaatc tggataagtt
     3721 aatggatgaa cttcgatttc tttgtctcct actttaaatg acaagtcatt caagaagttg
     3781 tagaaacgat gtgcaccttc gattgatgca cccattaatt taccatatcc tgtgacattc
     3841 aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct
     3901 tccaagatat aagccccaag taatgctggg atatctgcgc ctttttcaaa tacatatgtc
     3961 gcaaagtaag agataacgtc tctcatgcgg atatctttga tgacgatacc accggcagaa
     4021 tatggaacat agcccatttt atgtgggtca atcgttacag attctgcttc ttcgattgct
     4081 ttatacgcac tgtgtacttc ttctaagata taatctttgt tttctgtaaa gacattgtat
     4141 ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa
     4201 atcgcacggc cgtatccgcc ataagcagca tctacgtgta agtagaagta aataccgtct
     4261 ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac cgtcgattgc tccttcttct
     4321 gttgatccta caacaccgac tacacctagg attggtgttt tttcagctgc tagaccacga
     4381 acgatttttt caagttcgtt gatgtccatg cgatagttgt ggtcaactgg cactggaatc
     4441 acttggtcta aaccgatacc gatgatgtcg gcagctttta gccatgaata gtgtttagtt
     4501 tgtggaacca accattttcc taatttttct aaatgttttc cgctacgtgc tgagtgagct
     4561 ttgatttcgt cgattttttc tgggacgctg tctaacagat tcatgatttc ttctgttgac
     4621 aagttcatta attcccaatc gctttttcca gcaactagtt caggtgtcac ttctttcatt
     4681 gcaagcggca atgatttgat gttgcgggca taccaaagtc cttctaagtt tgctaaagaa
     4741 ccatcagcaa cgatgtggcc ccagccatct ttatagctca ttaattttgc aaattccatt
     4801 cctacttctt cttccatttg gcttgttgca ggagaagatt cataggcaac gttattacca
     4861 ttccatagca tagcaaaatt gtacgctagt aaagatggca tcaatgtttc agagttcata
     4921 tgaccccagt aacgtccggc attatgccaa ggaacggaat gggtacgcat ccgcgctgaa
     4981 atttctgata gaacatcctt tgttttattc acagtgtgtt caaaactagc actgcttttt
     5041 tcttctggcg taataatagg catgtcttga ggcatgtagt tttgacgcca gcctaaatgt
     5101 tcatccacca attcattcaa caaagcttta tagatttggc cgttctcagc tttgtcccca
     5161 atgaataggg catttaggtt tagatctttc gacaatgatt cactcatagt gaaaccctcc
     5221 taaaaaaata tttaaatttt gtcgagccct acattacacc tgttcttcta ttctgtaaac
     5281 taagttttgg cctgattttt aatcatattt taatatgtat caattatccg aattgttcac
     5341 acttttgaaa taattaaaaa aaacgaggga atttacaaat gtaaactccc tcgcaaagat
     5401 tgtattaacg acaaccagcg ttctatttta ggatgggttt caaaattgga atgttgtaat
     5461 agtatttccc tttttcaagg taaaaactta ctaaattcta atcaattact ttggccaaaa
     5521 agtaattttt cttacctttt cgaataacca caaacttacc atcgaattca tcggatggat
     5581 ttatgacaaa atttaaatcg gtaacgcggt caccgttaat acttatagct ccgtttgaaa
     5641 catcttcacg tgcttgacgt ttagatggtt caatttttgt ggaaacaagc agttccacga
     5701 tattttcagg tgtgcttgaa atttcaacgt tcggcatttt accgaatcct tgcgcgattt
     5761 cttccgcatt tagttcttta atatttccag agaacaatgc ttgcgttatt ttttgcgctt
     5821 ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg
     5881 cttcacgttt ctcaggttct gtctctactt ttttcgctaa gtcttcgatt tcttcttgag
     5941 acaagaaagt aaagaatttc aagtatttca ctacatcgcg atcatcttga ttcaaccaga
     6001 attggtagaa ctcaaatggt gaagttttct tcggatcaag ccaaacggca ccgcctgcag
     6061 tttttccgaa tttcgttccg tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt
     6121 ctgcttctgg tccttctaat tttcggatta ggtctaatcc agctgtgata tttccccatt
     6181 ggtctgctcc accgatttgc aattgcacat cgtatgtttt atgcagatgc aaaaagtcga
     6241 tagattgcag gatttggtaa gtaaactctg taaatgagat tcctacttcc aaacggctgg
     6301 caacgatatc ttttgctaac atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa
     6361 attcaagtaa agagattttt gacaaccagt cgtagttgtt cacaaatgtg atatttgcat
     6421 ctttaccaaa caattttctc atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt
     6481 ccatcgtttg caacacacgt tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc
     6541 cgccgccaat cagaatatat ggatgatggc ctgccaattg gaatcttttc atcatcataa
     6601 atggaatcaa atgtccgata tgcatcgaat caccagttgg atcaacgccg caataaagtg
     6661 aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg
     6721 cgtcgcgcca agttaattca tcaataatat tcattttact gacctccgtg tagttttttt
     6781 atgaaagcag gaacctgcta gcatgtgtaa ttaattattt cttcaggata ggataggaaa
     6841 taaaaaaagt cccttggcat ataagccaag ggacgaaatc tcgcggtacc acccaatttg
     6901 tatgtctaga tatccaacat acctctcgaa catttaacgc tgtcgcgtgg caatcactgc
     6961 caaagactcc tgaaatgtaa ttcgctttaa agcatgtact ggtttgcagc gaccaccagc
     7021 tttctgaaac attggcttta acaactactt cgtttcgatc ttcgtcttgt tattattaat
     7081 atgtgtctat catagagcgc aactttttct ttgtcaacag aaaaaggata ttttttttaa
     7141 attcctcatt ttttcttttt tgaatccgct ctaattccga acattttatt ttcaattatt
     7201 gttaaaagaa ataacttttt ctgtattttt tctgcaattt atcgagaagt cactttactt
     7261 tgcctcttat tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca
     7321 agggacgagg tatatggaaa aaaagcaaaa aattatttta ggagccactt ctgctttaac
     7381 tatcgttatt ttaattctaa gtttgttttt tgtcatccga tcatctacaa aaaaagaacc
     7441 aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa ccgacagatg ataagcaaca
     7501 actagttact caagcaaaaa atctggctca aagctacgat tatgaccaag cgattgcgtt
     7561 attggaaaaa gatagtaaga acggatctac tgaaacacaa cagcttcttc aaacactaaa
     7621 aaaggagaaa gaacaactga tcgactggtc tgatcctact cagatctctc atgtcttttt
     7681 ccacagcttg atcgtggacc cgcaaaaagc cttccactct caacaggcac aaggatacaa
     7741 agattatatg gtgactgttg aagaattcaa ccgttcgatc gaacaacttt atcaaaatga
     7801 ttttgtttta gttaatttga atgatttgat ccaaaaagac gaacaaggaa atctttcctt
     7861 taacgggctt gcgctacctg aaggaaaaaa accattggtt ctttcacaag atgatgtcag
     7921 ctattatgaa tatatggata attccggttt tcctagccga ttggtcgtat caaataacaa
     7981 tgaaatcaaa aatgtctata aagagaaaga taaagaagaa atcggtgact ttgacatggt
     8041 tcctctgatc gatgcctttg tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg
     8101 taccttggcc ctgacaggtt acaacggagt tttaggttat cgaacatcaa aatctcaata
     8161 tggagacaat gaaaaaacaa aaaaagagat taaagaagct aaaaaagtgg cggaacagtt
     8221 gaagaaagac ggctggagct ttgcttccca tacatggggt cacttgaata tgactcaagc
     8281 ttcactagaa gatgtaaaaa aagacaatga attgtggcag caagaagttg cacctatcct
     8341 aggaaaaaca aatattctga tttatccatt tggtgcagac atcagcgact ggcagcctta
     8401 ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa
     8461 tgtcgatgct tctacaccag cttggggaca attaggtacg gatttctatc gaaatgctcg
     8521 gatcaatatc gatggcattc gttttcaatc tgatttagac ggaaaaaatc ctattcttga
     8581 tccattcatc aatgtccatg atgtctatga caaagaagca cgcgaatctg cttgatttta
     8641 cgtcatttat gaaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat
     8701 gctttatttc ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata
     8761 tggaagcagt tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata
     8821 gaggatcaag aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg
     8881 gaaaagatca agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa
     8941 ttcttttttt tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac
     9001 tcgattgaga taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag
     9061 attttccgca atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac
     9121 ttcataaatc agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg
     9181 agcgttctca gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa
     9241 gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt
     9301 catttgtaga gcctcccgta attttaaact tttataatat atatagttca aatttattct
     9361 gaggtttatt ataactatct attgagaaag tatcaagcta gcaattcctt gtttatgaat
     9421 tcattgacag aaatttataa aaagaatatt atgtactaaa ataataaaaa aagttttata
     9481 aaaaggtata aaaccttttt attaaactat ttaatcttgt ttagtatata tttttagaaa
     9541 aaggagtggg gtttttatgg cagaaatcgt caaagtcaca catttgaaaa agagctttgg
     9601 tcgtttcgaa gctttaaaag atgtctcttt caccgtacat tcaggtgaag tcgttggatt
     9661 tatcggacca aatggttctg gaaaatccac gacgattcgg acacttttag ggattataaa
     9721 aaaagacggc ggaacggcaa cgatttttgg gcaagatgta tgggaagata gtttatctat
     9781 ccatgaacgt ctttcttatg ttccaggcga tatctctttg tggggaaatt tgactggagg
     9841 agcgatcatc gatctcttca tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga
     9901 actgattcaa cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa
     9961 tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat tcggatctct atttatttga
    10021 tgagcctact tctggtttag atcctttgat ggaaacgatt ttccaagaag aagttgaaaa
    10081 attgaaagaa cagggcaaat ccatcctact ttcttctcat attctcagtg aagtcgagcg
    10141 actagcggat aaagtcgtga tcatacgcga aggaacactc gatgaagaag gaacactcga
    10201 tgaattgcgt catttgactc gttcgaccat cactatagaa acagaagcag agaatagtca
    10261 cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc
    10321 tgctgataac gaagctttcg atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa
    10381 atttgaatct attccaccga ctcttgagga tttattcatg agacattatg aaggatgagg
    10441 aggaagcgaa atgactgata aattttcacg ctggtccacc cttttttctc aaaaccttag
    10501 ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc actgcttttt ctggtggttt
    10561 tgtccctgcg tttgaagaat tgacttccgg acaaggatta gctggattat acgaaacctt
    10621 gcaaaacccg gcaatgattg caatggtcgg accaactcca gcttcgaatg ctgctgagta
    10681 tactttagga gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt
    10741 gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg gtctgattga
    10801 acttattcgt tcctttcgag tcggacgtca agcaaactct ttagctgtca tcgctgaaag
    10861 tattctgatc aatttgcttt tagcaatatc gatcagctgt acgatgattt ttttccaagc
    10921 ggattcgatc actgcagaag gtgcttggct ttttgggcta tctgtaggta gtatggggat
    10981 tttggggagt gccgttgcac tgtttgctgc acaactgatg ccaagttcag ccagttcgaa
    11041 tggtctgtct ttagcgtttt taggtggact ttaccttcta cgagcaataa cagatgtagc
    11101 tgattcctcc ctttcgatgt ggaatccttt aggctggtct tatttgacct atccatttac
    11161 aactaacaac tggattcctt tgctttattt gtttttgttc agtctaatct tgataatcag
    11221 ttcatttgtt ttagaggaac atcgagattt gaacaccggc tatatccccg aacgtgaagg
    11281 aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt ttattattct atctaaataa
    11341 agggatgaca atcagttggc ttatcacttt tggtttgatg ggggcggcat acggctctat
    11401 ctacgggaat atgcaacagt ttttagaaag taacgactta atgaaacaga tgtttatcca
    11461 atctggcgtc tcaattgaaa aaaactttac aagcacgatc atggtcgtct tgatttgtct
    11521 agctgctatt ttaccaattg ctagcatcaa tcgtttattt acagaagaaa cccacggacg
    11581 tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa ctttattgga ccaatatgtt
    11641 actagctatt ctatttgcta tattggcaat cctgattagt tctggtgcac tagggctttc
    11701 tgctttggct tcgatgaagg atgcgaaaga catggatcta ttgatgtttc ttaaagccgg
    11761 gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt agtggctttc tattcggatg
    11821 gcttcctcga atcggtaaac ttgcttatat ctacttagct tactcctttg cactcaatta
    11881 ctttggcgga attttggatt taccagaatg gttttccaaa acagctgtcc ctagctgggt
    11941 tccacggatg ccaaacgatt cttttgatct ttccgtgttc cttacgttat catgtatcag
    12001 ccttgcactg ttcgtccttg gttctattgg ttataggcga agagacttac tagaaggata
    12061 ataaaaaagc atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa tccaacattt
    12121 ggaggtcatt tttttattta caagtcttct aactgctctt tatcaatgat ccagacttct
    12181 cgattctttc tttttacata accattctcc tctaataatt taaattttct agataaagtt
    12241 tcgggggtcg taccgataaa tgcagcaagt tctttcattt tcattggcag gaaaaagtga
    12301 tccttccctg tcgctttagc taagtcaagc agatagacag cgactcgttc ttctactttt
    12361 tccatcatca aaaaagttgt ctgctgctgt acttttgtta atttctcaac gttgattgct
    12421 aaaagtttca gactaagggc gggataatgc agtaagagtt ccgagaaatc tttttgtctc
    12481 aatatgcaaa tttccgtttt ttctagtgct tcgccaaata aagactcatt tggaacgcct
    12541 agcaactggc tctctccctc atatccgccc ggctctatta ctcgtaaaag ctgttccttt
    12601 ccgctagcag ataattgata cactttcata ctgccggagg ccacgatcac taattgaggt
    12661 tctccattcg gcgcaagaat ctgttctcct ttttccacat agtgatgttt gacaagtgcg
    12721 tgtacttttt cttgatcttg ctgtactaag tgattgaaca aaggaacaaa tgaaacacat
    12781 aaatgatcat gactcataat ctctcctcct tctaaacagc tatttctaat aaagaaacat
    12841 aacttccctt attccaactg ataattttct tttatcatag cataaacgtc aagcgaacca
    12901 gataccagca aatagagtgg atttcttttt gtcccttgct ttattttcct tcttttgaga
    12961 ccttaagtaa ttgattccta tcaatttttt aaatatttac tttttgaata atcaaaaaca
    13021 aaccttcact cgcaagccga gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc
    13081 tattgaaatt tttccgcacc tagcttttga tatagtggtg ctagttcttg taaggtttca
    13141 gcggttattt gctcaacgtg ttcgatttct tgcggataga agaatagttt acccatttga
    13201 aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc ggacaaatag
    13261 tcagacaaag ctaagtggtc gtcaagttca tgttctggtt gcggccatat gtaataatcc
    13321 ttgatatcga ctgttttgct ccactctttc agtatcgcaa gcaactggtt atacgctgca
    13381 atgctaccat atcgttcttc gcttttataa tgttggtaca tcaaatagat ttggtattgt
    13441 ttttgattca acaaaactgc tagacaggca ttctcgtctt gacgagattc gcttcggtac
    13501 gcgctccaaa aatgactgcg taaattccag ccattcgtcc agctttcgat tttaggtttg
    13561 ctcatgccgt acatatcagg caaaagtgca gctgtctgga gctgaatctc tttccatttt
    13621 tgccagtgtt ctttatattc tttcttgatt tgttcaatct tatctgttgg atggctctct
    13681 ttgagctgtt tgaatgcgta gattggtcga tcaaacaact gataatctga ttttccaagt
    13741 tccattgttt cgcctctttc cgttatcttt tgtattctac ctgaattttg cgatatggac
    13801 aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata agaacagcca aacaaaaaac
    13861 tgcgatgagc atcaccgcag ttttattgaa cttaaggttg attgatcaca tctaaaagcg
    13921 ttgaaaataa aatggcacta gaagcagctc ctgggtcgat atgtccgatt gcacgctctc
    13981 ctagatagga agctcgtcct tttttagcta ccaagacttt ggtgtgttct tttgcctggt
    14041 caatgatttc ttttgttaaa ttccctgcct tcaatgcctc tgtgactggc agccaaacat
    14101 ctaacatcgt tttatcgtca gcttctgcct ttcctcggct ttgaataccc gctgttccct
    14161 gctcgatcac ttcgcctaat ttttcttgag aatcaataga atcgacctct tttgtcgctt
    14221 tcatcatgtt catgaatgca gatccgtata acggaccaga agccccaccg actttcgata
    14281 tcatcgccat agagaatact ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa
    14341 aagcttcttc ataagctgaa acccctcttc ccatgttatt tccatggtct ccatcgccaa
    14401 tcggggtatc tagatcactg agatattgtt tgttctgatt gattgcctct gcaaattgcg
    14461 tgagccattc ttggatttct tgaacggtta gtttcatatt atcacccttt tatcttgaga
    14521 ttttttacca tgaaatcgtc tgtacattgc tttctaaaga atctttccat tgtgtctccg
    14581 tcaagtcgat cagtgttaga gaaagacctt gcatatctag tgaggtcata aaattaccga
    14641 cttttcggaa aactacattg acgcctcttt cttccaacag agctaaaaca tcattcatga
    14701 acacgaactg ttccatcaaa ggcgtcccgc ccattccgtt gactagtaca cctgcttctt
    14761 ttggtaaatt cgagtaatca tctaaaatct tagtaacaag ttcctttgcc agtactttag
    14821 aaggctgcat cttttctcga cgatagcctg gctcaccgtg gataccaaca ccatactcga
    14881 tctcatcttc tcctagttcg aatcctggtt tccctacttc tggtacagtt gctgctcgaa
    14941 gcgctagacc gatcgtctta gttgcctgta cgaccttttc tcccagttct ttcaactcag
    15001 ctagggaagc accgtttctt gctgcatcgc ccacgatttt gtgtacaagg acagtacctg
    15061 caactccgcg ttttccagca gtatacgtac tattttcaac ggcgatatca tcatctacga
    15121 tcacactttc tacttcaata tcgtccatag cagctaattc ctttgccata tcgaagttca
    15181 atgcatcccc tgtataattc ttaacgatca gcaaaactcc ttttccacta tcggcttcct
    15241 taatagccgt ctgaatctga tctggagttg gagaagtaaa cacatcacca agcaccgctg
    15301 cacttaacat cccttcccct acaaacccgg catgggatgg ttcatgtccg cttcctccac
    15361 cagaaaccaa accaacttgt tccgctttct gattcttggc tacaacacgg gaagattcta
    15421 cacgatgaac aagttctgga tagcttttta ctaatccttc cagcatttct tcgacaatat
    15481 tgcttggatc attcatgatt tttttcaatt tattcccatc ctttctttcc ggcattttgt
    15541 gcttctgtta agatttgctg agtatcatct gttataccgg cagtgatggc tgctgcaaaa
    15601 gctccttcca ctaaaggcgc atccaccaga tgataacgtt gctgctgctc ttcttccaac
    15661 atgtcttttg ccagttccga gttcaatacc gcgctaccga tatccgcaaa aacaaaataa
    15721 ctagctgcat cgcttgattc attgactgct tctacgattt tcatcgggtc tgatccgatc
    15781 tcgccattta ttccgccgag agaaaaaatt tgcacctctt cggatttttg catctgctca
    15841 atcatttctt taataccatc tgttactttt tgactatgag aaataagcaa aatacttttt
    15901 ttcacaagaa tcttcctttc ttttacatag ttgctaacga ttggtttcgc tttcattaat
    15961 gaacaattca agtatataat aaatttttcg tttgtcaaat taagctagac aaatcatctt
    16021 tacctacgta actcaaaaat acttccaatg gtgtgcgata gtgtaatgat tttctaggaa
    16081 tatgatttcg ttttgatgcc accgattgga taaaggtttc ggatacctta ttaaaatcca
    16141 tagctttata taagccatct ttacgtaata agccattcga atgttcattt agtcctcgtt
    16201 gagaaggtgt tcctggatca gcgaaataaa tcgaaatatc attggtatta ctaatctttt
    16261 tccaattcga gaactctttt ccacaatcaa atgtgatgga tttaaacaag tgtttcggta
    16321 cagattgaaa ccattgattc agtctattct caatatctat agcttgccta ccagcgggtt
    16381 tcaaggtgat gatcacttta gataatcgct caactaacgt gatcaccgca cttttatgtt
    16441 ttccaccgac aatggtgtca ccttctaggt gaccaaactc atgattgaac tgtatataat
    16501 cagttgttct atcctgaatc gaacggcgaa aagcttgttt tcctcttttt tctttatgtc
    16561 cgtttggttt tcttttccct ttcattggta aatcgtgagg gtcaaaggtt ttatctttaa
    16621 acatccgata tagtgtacgt accgaacaag caataggaaa ctctgctcga ccaataagga
    16681 catctggagt ccacccttga gccacctttt tttgaatgta ttcttgttgc ccatctggta
    16741 acacaatcgg acgtcttccg cagttacgtt tgtttttctt atattgttca tagtagctta
    16801 ttgcgcttcc accagattta agaaaacgat agacattata aatggtttgt cgagaacgtt
    16861 gtaccagatg ggctactttt gaaacagagt gtgattgatt gaaataagct tctatcatta
    16921 cgagttcagt tggtgtaaga tgggtatagg tcatttgtac tcactcctta taattctttc
    16981 gacggaacta ttttgagtgt agcacaaatg acttttttag ttgtctagct taattttaca
    17041 atcggcgatt tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt
    17101 aattaaaaga gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag
    17161 ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta
    17221 aagacttgga ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg
    17281 aactaatggg tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag
    17341 gagctgatgc gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca
    17401 tgctgatggt cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt
    17461 ttaataacta aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta
    17521 acttcgaggt actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg
    17581 taagctgctg cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt
    17641 tcatttgttt ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact
    17701 gtttctgtcg tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt
    17761 attcgttgga aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt
    17821 tgtgcttcgc tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt
    17881 tgacttagct ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt
    17941 gcttgttgga tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg
    18001 gcaagctttt tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa
    18061 tcttgatcga ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct
    18121 tcgagttctt ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta
    18181 gaactattta ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt
    18241 acattggaag tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatggtt
    18301 gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag
    18361 aaaatactgg aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt
    18421 cccatcctta ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca
    18481 taaacacaac aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg
    18541 ggcttttcca tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg
    18601 tcacgatcgt tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa
    18661 ggtatttcaa ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc
    18721 ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt
    18781 cgtagtttcc ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac
    18841 aaaagaatat tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa
    18901 tatgtttaaa aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac
    18961 cttttgccca acgttttctg tgtcgatata gcatcttcaa cgtcacaggt acttccatga
    19021 aaaagataat ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa
    19081 tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt
    19141 ttcgatacat cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg
    19201 ttcgtttaat gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt
    19261 tggtacgatt ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc
    19321 gaatgaaata attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat
    19381 tgcttaaaat caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc
    19441 ctttattttt ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta
    19501 atatttctgg tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat
    19561 aattcaattt cgtcatcaaa tactcgattg tatcttcgat cacaatttct tcattatggg
    19621 ctggcaccat gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt
    19681 gcttatgttt gtataaaaac acataacacc agacaccaat aaaccataca aatccgccta
    19741 aaatcggata agaaacgaga aaaaaataca taatagacaa acaagtatct aagaataaat
    19801 tacctgtatg ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc
    19861 aatgaatgtc gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct
    19921 ctcttctttt gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt
    19981 caatttttca tcttcata
//
LOCUS       DACGLH010000048        19524 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.052, whole genome shotgun sequence.
ACCESSION   DACGLH010000048 DACGLH010000000
VERSION     DACGLH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.052"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            153..932
                     /locus_tag="IXT21_001877"
     CDS             153..932
                     /locus_tag="IXT21_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869263.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            978..1922
                     /locus_tag="IXT21_001878"
     CDS             978..1922
                     /locus_tag="IXT21_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4869264.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1938..2717
                     /locus_tag="IXT21_001879"
     CDS             1938..2717
                     /locus_tag="IXT21_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ4869265.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2729..3427
                     /locus_tag="IXT21_001880"
     CDS             2729..3427
                     /locus_tag="IXT21_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ4869266.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3455..4219
                     /locus_tag="IXT21_001881"
     CDS             3455..4219
                     /locus_tag="IXT21_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ4869267.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4263..5714
                     /locus_tag="IXT21_001882"
     CDS             4263..5714
                     /locus_tag="IXT21_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ4869268.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5746..6882
                     /locus_tag="IXT21_001883"
     CDS             5746..6882
                     /locus_tag="IXT21_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4869269.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDLHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6882..7988
                     /locus_tag="IXT21_001884"
     CDS             6882..7988
                     /locus_tag="IXT21_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ4869270.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            7994..9016
                     /locus_tag="IXT21_001885"
     CDS             7994..9016
                     /locus_tag="IXT21_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4869271.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9038..10093
                     /locus_tag="IXT21_001886"
     CDS             9038..10093
                     /locus_tag="IXT21_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4869272.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10711..11946
                     /locus_tag="IXT21_001887"
     CDS             10711..11946
                     /locus_tag="IXT21_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869273.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12030..13118
                     /locus_tag="IXT21_001888"
     CDS             12030..13118
                     /locus_tag="IXT21_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869274.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13115..14536
                     /locus_tag="IXT21_001889"
     CDS             13115..14536
                     /locus_tag="IXT21_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ4869275.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            14629..15288
                     /locus_tag="IXT21_001890"
     CDS             14629..15288
                     /locus_tag="IXT21_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ4869276.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            15307..16890
                     /locus_tag="IXT21_001891"
     CDS             15307..16890
                     /locus_tag="IXT21_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAQ4869277.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            17025..17603
                     /locus_tag="IXT21_001892"
     CDS             17025..17603
                     /locus_tag="IXT21_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869278.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            17549..18067
                     /locus_tag="IXT21_001893"
     CDS             17549..18067
                     /locus_tag="IXT21_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869279.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            18075..19115
                     /locus_tag="IXT21_001894"
     CDS             18075..19115
                     /locus_tag="IXT21_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ4869280.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            19364..>19524
                     /locus_tag="IXT21_001895"
     CDS             19364..>19524
                     /locus_tag="IXT21_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4869281.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         7120 a         2468 c         3830 g         6106 t
ORIGIN      
        1 agtttgtggt atgattatat agggaaaact tgtttacaaa tatttctgtg aatatattag
       61 tttaattatg tttttttaaa taaaaagctg tcatttttat gtaagtgaat tatatcttaa
      121 tttatctatc atcaatactt aggagtgaat ctatggaaaa gccattcgtc aagtcattga
      181 ctgctgtggc ggtaggtgca ggtgctattg cgatctgttt gtttggatac catatcaata
      241 atcagcggca acatcagcaa cggatcaatt atgcagaatc tgccatcacc aatcaaaaag
      301 acaccttgac ttctttatca aaagaagtag acaagctgta tagtacaaaa gaaaaaattt
      361 ttctaaaccc tgaaataaca gaagaaacaa tcacaagttt gtcaaataag ctatcctctg
      421 tcaaactttc agcagatgat tacgacatca aagaatcaga attgcctaaa gaagctgttg
      481 caatccaaga tgagaagaaa gcaatcgtga cacagttaga tagtgcagaa tcaaaactga
      541 agatccagac agctgtcaac gatttattta ctaaaaatgt atcaaactgg cagcaagctg
      601 tagacgatgt catcatcaaa gaaaaggtga caagtgcaga tgtcgctcgt gtacgtgaaa
      661 atatgagctt ttttaaagat agtccttgga aaacggttgt tatgcaatat ctaggattcg
      721 ctgatattca gatcgcgcaa gtcacgcaac tagatcagtt attcgatacg atgttaaagg
      781 atggtcaagt gactgccacc gctacttacg atcaatattt gacggtgctc agtcaaatag
      841 agcagatccg gaatgaaaaa ataagcgcag catacgcaac aaaagcacaa actgttgctg
      901 agcaaatggg ctatagcaat ataagctatt gacagttaaa acgttgattc aagcaaggtt
      961 agcaaacgga ggaaactatg gcaagacgaa gaagaaaatc aagagggaaa aaaatatttc
     1021 tgattatttt agcagttctt atcgtattag tattagcaat tgtaggagta ggagcaaaat
     1081 tatatctgga tctctcacat tctattgaga aaacttacga atcagtggaa cgaacccaag
     1141 gagaacgaac gaaaccagtt gatctggaca aacaagaatc tttctctgtt ctattgatgg
     1201 ggattgatac tggagattta ggtcgggtcg atcaaggaag atcagatacg atgatggttg
     1261 ccacagtcag tcctgaagac aaacagacaa cgatcgtcag cattcctcgt gatacgtatg
     1321 tcgacatcgt aggtcgtgac acaaaagaca agatcaacca cgcttacgca ttcggcggag
     1381 ctgccatggc gatggatacc gtacaaaaat atgtagacat cccaatcgat cactatgtct
     1441 ctatcaatat gaaagggcta aaggaactcg ttgatgcagt tggtggtatc gaagtcaata
     1501 atgacctcac tttctcacaa gatggctatg actttccaat cggtaaaatt acattgaacg
     1561 gggatcaggc attatcttat tctcgtatgc gttatgaaga cccaaatggt gattacggta
     1621 gacaagggcg ccaaagaaag atcgtagaag gcgtagcgaa aaaagtattg agtctaaacg
     1681 gtgtcagcaa ctatcaatcc gttttgaatg ctctagagtc gaatatgaaa acagacatga
     1741 actttgatga aatgaaaaaa attgctttag attatcggga ggcatttggt acgattaagc
     1801 aagatcagct gcaaggagaa ggcttcatgc aagataatat ctcctatcaa aaagtcagtg
     1861 atcaagaact tgatcgtgtg aaaaaagaat taaaagatca aatgaatcta gagaataaat
     1921 aggaaagtgg agaaaaaatg gaagagacaa tcagcttgaa agaaattttt gaaacattac
     1981 gcaaacactt ggtaataatc atcatctcaa tgtttgctgg cttggccatc tcaggaatcg
     2041 taacattttt catcttgaca ccaaaataca gttcacaagc acaattgatc gtgacactgc
     2101 cacaatcaga cactgcaaat gtcaatgatg tcaatacgaa cctgcaaatg atcaatacgt
     2161 ataaagatat gatcgtcagt gatttagtgc tcaatgaagt caaagatcgt ttagaaacag
     2221 aagacaatgt gaaaatgact gctgaacaaa tcaaagatgc catctcagtt aatcaggcag
     2281 aaaattcaca aatgttctcg attcaggcaa taagtacgaa tgcagtggct gctcaacaga
     2341 tcgctaacac aacttcacaa gttttccaag agaacgcaaa agatgtgatg aatgttgata
     2401 aaatctcgat catttcaggt gcagttgcca atacgacacc tgtttcgcca aataataaat
     2461 tgaatttaat gactggctta atgttaggaa tgatggtagg tgtaggtctg gcattcttat
     2521 tggaattact cgatcggaca gtgaaagatg aaaaatatgt gacagataca ttaggattcc
     2581 caatcttagg aacagttcca gaaatgtcag cgaaagaatt gaatgctggt atccgtaaaa
     2641 aaggaccagc tcagacacaa ccaaaagcta caaacgataa aacagcgcca tcacgcagaa
     2701 gccgctcaag agtctaggag gaaagaaaat ggcacgaaca cagaaacaaa aagcagccca
     2761 aactcatgca gtcagtttgg tcacactcac caatccctct tcaccaatcg ctgaacaata
     2821 tcgtacgatt cggacgaata tccagtttgc ctccgcagct ggtcagcaaa tcaaaacgat
     2881 tgttgtgact tcatcaggac ctggagaagg gaaatcgacc actgcagcaa acattgccgt
     2941 tgtttttgcc aaatctggtc aacgggtgct gctagtcgat gcagacttac gtaagcccgt
     3001 catctataaa acgttcaagt tgaacaacgc aagcggatta agtactgttc ttagttcatc
     3061 aggaagtgta gcagatgaga tccaacgaac accagtcgac aatttgtcga ttctgccaag
     3121 tggacctaaa ccaccaaatc catcagaact actaagttcg ccaagaatgg atcagatttt
     3181 agcagaagca agacaactat ttgatgtagt gatcttcgac atgccgccag tcgttgccgt
     3241 aacagatgca cagatcatgt cttcaaaaac ggatggcacc ttattggtcg tacgggaaaa
     3301 tacgtcacga aaagaatcat tgactaaagc aaaagaactg ttagatatgg tacaagcacg
     3361 agtcttaggt gtcgtataca acggagcaga acacagcaaa gatgctggtt attactacgg
     3421 aaattaagga ataagaagag aggagacaag agaaatgatc gatctacatt gccatatcct
     3481 accaggaatc gatgacgggg cagaaaactt agaagcaagt attgcaatgg ctgaaaaagc
     3541 gattcagcaa ggcattacgc atattttatg cacaccacac cacaataatg gaaaatacag
     3601 caatgaaaaa tcacaagtca tttctctagt cgcttcgtta caagccgaat tagagaaaag
     3661 acagttgcct ttgacgttat tggaaggaca agaagtacgt attaccggaa cgctcatcga
     3721 agacatccac cgagatgaaa ttttatttac agatttagat gatacatatc ttctgatcga
     3781 atttccgaca ctggaagtgc cgttatatgc agaacggctt ttttttgagt tgttagaact
     3841 aggaaagata ccagtgatcg tccatccaga acgcaacgcg cattttcgag aagacccaaa
     3901 tcatttgctt ccgttcctag atatgggatg tttggcccaa cttactgcgc caagttatgt
     3961 tggtgtattt ggcaaagata tccagaaaac agccaaaaca atggtcaaac ataatctcgt
     4021 gcagatggct gcttctgatg cacacggggt ctctaagcgc accttttact taaaagagtg
     4081 ctatgagcag attgccaagg agtttggtaa ggaaaaagta gtacagatga aacaagtcgc
     4141 aaaggatctg atcaacgggg atgagatcaa ttatccaatc tatgaagagg taaaaaagaa
     4201 gaagtttggt ttattttagt tctttattcg tagataaaaa ggaaatgagg agagagtgag
     4261 agatgtatag aaaaaactca agtggttggt ctaaacatat tgattttatt ttattagact
     4321 tattttgtgt ccagcttgct ttttatatat catatgtaat gcgacagggt gactggaatc
     4381 cgtatgtgat tcctatttat cgccacatgg caatttttat agaacttgag aatcttacga
     4441 ttatattttt atttgaggca tataaaaatg ttttaaaaag aggatattat aaagaggctg
     4501 tagcttctat aaagcaatct tttatgctgt tacttgtgtg cagtttatac cttattacaa
     4561 tgcaggatgg aaatgattat tccagagttt ctttatttat aatgggggtg gtgtatggat
     4621 tattaacata tataattcgt atactttgga aagggttgtt gcatcaccgc atgaaaaacg
     4681 gtgaagatgt atcgttagtt attgtaacat ctcaaaagat tgcaaaaaat gtagttcgta
     4741 atcttaaaga gaagaattat ggcatgtata aacttagtgg tgtgattgta gtagacagag
     4801 atatgtatgg aactaccatc ggaggtgtcc cagtagttgc aaatgctgat aatgcagcgg
     4861 aatatctttt acagaattgg gtagatgaag tattcatcaa tgttgacgaa agtgttccat
     4921 atcccaaaga attaatcgcc agatgttcgg aaatgggact tgctgtacat atgaatctta
     4981 caaaagtgtc tgatagtaca cgtggaaaac aatttattgg ccgagttggt gattatactg
     5041 ttattacatc tagtattaat gttatgacga tgaggcaagc atttataaag agaacgattg
     5101 acattcttgc tggaattatt ggatgtattg caacaggtgt tatttttatc ttcgtagcac
     5161 cagttattta catcaaatca ccagggccga ttttctttgc acaagaacgt attggtcaaa
     5221 atggtaagac atttaagatg tataagttcc gtagtatgta tatggatgca gaagaacgca
     5281 aagcagagtt gatgaaagaa aataagatga gtaataatct catgtttaag atggattttg
     5341 acccaagaat cattggcaat aaaatacttc ctgatggaac aaagaagaca ggaattggac
     5401 aatttattcg atctacatca ttggatgagt ttccacaatt ttataatgtc ctaatgggaa
     5461 aaatgagcct ggtggggttt agaccttgct tgaagtcaga atatgagaag tatgattttc
     5521 atcatcgtgc gagaattgcg atgaaacctg ggattactgg aatgtggcag gtgagtggta
     5581 gaagtgatat cacagacttc gaagaggttg taaaacttga tgcagaatat atcaggaatt
     5641 ggagtatggg attagatttt agaattttgt ttaggacagt caaaactgtg cttcatagaa
     5701 acggttccat gtaagataaa gatactatag aaggagtaaa acgtgatggc aagaaaaaaa
     5761 ttaaaaatct gtatgctagg tcataaaaga attccatcaa gagaaggtgg aattgagata
     5821 gtggtagaag aattggcaac gcgaatggtt gcacttgggc atgaagtgac ttgctataat
     5881 cgaagtggtc atcatgttag cggaaaggaa tttgacttgc ataaaaataa agaatataaa
     5941 ggtgtaaaat taaagactat ttttacatta aatataaaag gaattgcagc aatgagttct
     6001 tcagtatttg gtggaattag agcagcattc ggaaaatatg atgttgttca ttttcatgca
     6061 gaaggaccat gtgctatgtt gtggttgcca aaactttttg ggaaaagatg tgttgcaaca
     6121 attcatggtc tagatcatca aagagaaaaa tggaacaagc ttgctagtac atacatcatg
     6181 ttaggggaaa agtgtgctgt aaaatttgca gatgaaataa ttgtacttag tgaaagcgtc
     6241 caaaattatt ttgaagatat atatggaaga aaaacaaggt ttataccaaa tggcgttaaa
     6301 aaaattgaaa taaaatcagc aggattgatt acagaaaagt atggattaac aaaagattct
     6361 tatattttgt ttttggggcg attggttccc gaaaaaggaa ttcggtatct aattgaggca
     6421 tttaaagatg ttcagacaga taaaaaactt ataatagccg gaggaagctc tgatacagat
     6481 gaatttgcaa atgaactaaa ggaattagca aaaggtgatg agcgaattat atttactgga
     6541 tttgtacaag gacaggaatt agaagaatta tatagtaatg cgtatatcta tacacttcca
     6601 agtgatttag aaggaatgcc attaagtttg ttagaagcaa tgagttatgg gaactgttgt
     6661 atagtttcaa atatctcaga gtgtacggaa gtagtagaag acaaggctat gatttttaag
     6721 aaatcggatg taagtgattt aaagataaga ttagaagagg catgcaatca atcggaaatg
     6781 gttaaagttt tgaagaatca agctactgag tttatttgta gtaaatataa ttgggataaa
     6841 atcgtacaag aaacattaaa tttgtatagg ggaaatatat aatgattcga atattacagt
     6901 gtgtgaatga tatgcatcgt gcagggttgg agacgatgct aatgaactat tataggaata
     6961 tagatcgaac taaaattcag tttgattttt tgacacatcg tccatataaa agtgattatg
     7021 atgaggaaat aataagtctt ggaggaaagg tctattatgc accgagactt tatccacaaa
     7081 attatcctaa atattttaaa tggatggaac aattttttaa ggatcatcca gaatatgaga
     7141 ttgttcattc tcatattgat gcgatgagtt atttaccttt aaaagctgca aaaaaagctg
     7201 ggattccgat acggatagca catagccata atacatcgat agatagagat tttaaatatc
     7261 ttttaaagca atattatcga tgtaagttaa cgtctgtttt gacacatgaa ttttcatgtg
     7321 gaaaagtagc aggtgatttt ttgtttcgaa atgataaatt cactttgatt ccaaatgcag
     7381 tggatgctaa aaaattttat tatgatgaaa gtattagaaa tgatgtacga atgaaattag
     7441 gaatttcaag tcagacgttt gtaatgggac atattggaag aatttcatat tctaaaaatc
     7501 atcgtttttt gattgaaatt tttaaagaat ttcacgctat taagaataat tccgtattat
     7561 taattattgg aactggtgat atggaagaag aaataaaaaa ttatgcaaag aaaagtggca
     7621 tagatgatga tattaaattt ttaggaaata gaaatgatat agaaaaattt tatcaagcat
     7681 ttgacgtatt aatgttgcca tcattatttg aaggggttcc gttagtgggt attgaagctc
     7741 aatttgcaga tttaccgtgt ttcttttcgg agaaagttcc tacagaagtt gctttcagtg
     7801 ataagaccaa tttcataagt ttaaatcaat cggcaaaaga atgggcgaga gagattgcaa
     7861 atgtagatat ctctcatcga gattcagaac gttcgatttt aataaaggca gattataata
     7921 ttcaaacagc atacaaaatc ttagaatcaa aatattatga gttatttgaa ttaatacaaa
     7981 gaggttaaag aatatgacac agaaacattc aagtataaaa agaattatac atcgcatatc
     8041 cgaagaattg aatgcaacaa gacgaattgc aaagataaca tcttggaagg aagcatttgc
     8101 cacgttacaa gctaaaattg atattcaagt catgaatcgt aatggatata atgaaagtaa
     8161 aaaacagaag gaacatttat taaaaaaaca tgaggtaatg ctgaagtatt atgaaaagac
     8221 tttttcagat tttttgaaaa catatgatta tagtcaaaat caaataatta atcaatcaaa
     8281 atcaccatat tctaactgca tttgggtttg ttggtggcag ggattggatc aatcgccgga
     8341 aattgtaaaa gcttgtgtaa aatcaataaa acgaaatgca gggaaacata ctgttataat
     8401 tttaacagaa gataattata gacaatatgt tgatatacca aaatgggtgg aagaaaagaa
     8461 aaacaaagga atcattacga gaacaaatta ttctgattta ttaagattat ctttgctagc
     8521 aaaacatgga ggtttgtggt tagacgccac attttattgt acaaaaccaa taccagatga
     8581 gtattttgat atcgatattt ggtctattag gaggcctgat tatgctcacg catccgtagc
     8641 atcaggatat tttgcgggtt attcattagc atgtaatcaa gagtctagat ggatgtttgc
     8701 tataattaga gacttttttt taaattattg gaaaaataat gatatgatgg tggattattt
     8761 gatgatagat tatatgattg ttttagcaca gagaataaat catgaaatag cacagacatt
     8821 tgataaaata cctagtaaca atccattatg tgacgaattg attaaaaatt taaataatat
     8881 atatgatgaa aaagtatgga acttattaaa gaaagatact ttactgttta agttgacatg
     8941 gaaacaatct tttaaaaagc aaaaagatga taaagttact ttttatggga tgttggtaga
     9001 aaacaaatta ttataaatat tagaggtata aaaagttatg aaacacaagg taagtgtgat
     9061 agtaccaatt tacaatgttg aaaaatattt aagaaaatgt gtggatagtt tattgagtca
     9121 gacattaaaa gagatagaaa tcgtattagt tgatgatggt tcgccagatg cttcggggga
     9181 aatagcagat gagtatcaaa aaaaatattc taatgtaaaa acaatacata gagaaaatgg
     9241 ggggttagga ccggcaagaa atacaggaat tgagaatgcg acaggagaat atgtggcttt
     9301 tcttgattca gatgattggg tagaaagtga tatgtatgaa aaattatact tagttgcgtc
     9361 caaagaaaat gctgatatag tagttagtgg acattgtgat tttgcgaatg acaaagctat
     9421 ggtgaaaaag caacatcctt tgagtgggaa tgtatatgat acgcctaata gtattaaaaa
     9481 tgtacgaaaa aatttatttg gacatagtcc aaaagatcaa gaagtagagg ccttcccgat
     9541 gtcagtatgt atgtctttgt ataataaaaa tctgctagat aaatatagac taagatttga
     9601 aaaaatttta tctgaagata caatttttaa cttgaatgca tatgatgtcg caaataaaat
     9661 tgcttttaca gaatatacag attattgtta tagaaaagaa gaacaagagt ctattacaaa
     9721 aagtttatct ccaaataagc agacggaatt taaagaattt ttaataactc taaaagctat
     9781 agcatcgaaa gaagatttag aatgtcaaat cagagcaaaa cgtatggcaa ttgattattg
     9841 taggctttat gtgggcttag ttgatgatgc aaatttggga attaaagaga aaagaaagta
     9901 tataaaaaaa tttattgaag atgagggatt aattcaatgt tggaagggat atcctacaaa
     9961 tacgctacca tttcaacaaa aattttttca taaaatgctt gaatgtaagt gttatacaat
    10021 attgttgttg ttaagtagaa ttagaaagat aattaaaaat atgaccagaa aaaaattgtt
    10081 tataatatta taaactaaaa gtatatcaaa taatagaaat taacaagtta caaaaaaata
    10141 ttataaagaa taatattttc taaaaagtaa actatagtaa tgtggattat caccttccaa
    10201 cagtatataa aatgagcgta aaaacggcta tttttgcctt aacaacgatt tttttgtata
    10261 caaatacgat atgctcatta attattggaa tttgtagtgc tgtttaaatt aatgaaggtg
    10321 taattatatg agtgatcatt ttgaaaaagt ttgttgagaa gatgtgcttc tagtcatgtg
    10381 atgggagaaa ataatttaac tgttagagtg aatagttaaa ttattctggt tatatgaaaa
    10441 aagtagtttc taaaataatc ttgcagggat taacttaaag aaattgagag aatagtttct
    10501 ttgaaatttc tatatacaat gagaagctga aatttacttt tatttgatga ctggaaatag
    10561 aaattaaata tattgtattg ttacattaaa gaaaacttgt atggaaaaat cagtacaata
    10621 ccattattta atgttcttca cttaaagcgc aaaaaatatc agtttattaa actcgtatat
    10681 agaacaaata tatgtagacg gggcgaaacg atgagtgtaa aaaaaatgct atttatggta
    10741 agtacaataa ttgtattaac atcccgattt tggggaatta atactagttt atctatgcga
    10801 tattttatta tggcaatatg ggatttatat tttatgttaa gctatttttc ttatcgtgag
    10861 aaaacatata aaaaatacgt tatcaagaat aacttttggt tgactataat gccgtttgtg
    10921 atattttcta tctatactct aataatatgg ggaattggtt caaatgcaga atttgaaaat
    10981 tttataaagt tatgttccac gttgctttat ttaattcttg catatggtta cgcatgtaca
    11041 gcattttact tatttaaggc agaaggaatt gattttattt tttggtcagg tgttatttca
    11101 tatatgagtg gttcagtttt ttatttaatt gtatcttatg gtttaaatgg tttaatttct
    11161 tatataaaaa gtcttataac aggtgaaaca aatactgcaa attttgctat ggaggtacat
    11221 gatttaactt ttgcgatggg ggtttttttc ctttattacg tattttttga aaataaaaat
    11281 acaaaaaaac ataagataaa aattatcgta tctttaattt taattttcat gggattgaag
    11341 agaattgaac tgttagcttt aagtggtgca attctagtgt attatgtttt attaaagtgg
    11401 ggaaaaaaga ttcaaatccg tgcattagtg tgtactattt gtgcaactgt tatttcgtta
    11461 gtgtttattt acattataga tacaggaatt ttggcattgc ttatgtcaaa tttgggcatt
    11521 acagatgacg gtgcgaggct ttcattttat ctatattcat ctaaatttta tgaacttggt
    11581 ttaggatatg taggaacagg atggacatgg ttttcaaaat acttttttaa tctttattta
    11641 tcgcacttta ggttaaatgg tggaagaatt gcagcaagcc tacacagtga tatattaact
    11701 atttatatag aagttggatg tatagttttt attatatgga tgttttggtt attttttgtg
    11761 aaaccgataa gactaactaa aaaatttgga ttgaaaacag gggagtgtgt attattatta
    11821 actatatata tgtttatatt atatttgacg gataatacgc tcacttatcc agacactcaa
    11881 atgatttttc tcttagtacc tatgatagtt tcgtgggttg ataatcaaca acgaatatcg
    11941 gaatagatag ctaaagtata ttttaattat gaatttataa ttagttgtaa aagttaatat
    12001 ttaacggaca ggaagtaggg tgaaaaaatt tgaaaaaaat tttagtgtta gcaaatacat
    12061 attatcaaat aatagtagca atacaaatga aaaatacaat tttttccgat gagtatgttg
    12121 aaattattat ttctgatcat tctaaaaaca ctaaagaaat tgcgaaaaaa atagaagaaa
    12181 aaaaagtgtt tgatggctgt cattatttag aaagcaaatc ttttgatgat aatattgggt
    12241 attttcaaaa gattaaggac tgtcttcaga tttcttgtgg taaatcaaat cgatatgcct
    12301 attatttgaa aaactgtaaa agtgaatatt ttgatgaatt tatttgtttt tcaatgggag
    12361 aggtttggtt ggaaggaata tatgcttgct tatatcgaaa taataaaaat gtacaggtat
    12421 caattttaga agaaggtatt ttaagctatg gagttgacat tattccaaca gcaagaacaa
    12481 aagctatcgc agtactcaat tacttaaaaa aaaggactcc actatataac aaaagctata
    12541 atttttattg tttttttcct aatatatata caggaagaga gaatacaata caaattccat
    12601 tagttacagc tgggtcaaga accgcattgc aactttcgga tatttttaac cctatgatag
    12661 atatttataa agaaaaaaaa tatatatttt ttacgtctgt ttatgatttt gagggcggag
    12721 aacctgtcgg agaatatgaa ttagtatgca aaatagcaga cttagtagga aaagataatc
    12781 taattgttaa gacgcatcct agagatgtaa gaacaattta taaagataat ggatttatcg
    12841 aggataaaaa ttcatcaata ccttgggagg cgattcaatt gtcaggcgat tttaacgata
    12901 aaatatttat gacaattaat tcaggaagtg ttatttcagg tagtacaatg tctgcaaaac
    12961 ctgtacgcac gtattatatg tataaattgt gtaacataga aggaaatccg tcttgcaaaa
    13021 agaatgcgta tgatattgag cgattgctta agcaaaaaac gatgaaaaac attttaaaga
    13081 atgtgaaaat agcagaaagc ttagatgata tactatgagc aaaactaaat atttaattaa
    13141 aaatatggga atattaacaa taagtaattt ttcttcaaaa attttagttt ttttgttagt
    13201 ccctttatat acaagtgtat tatccacaac agaatatggc acatatgatt tgacaatttc
    13261 aacagccaca ttattgtttc caattttaac cttaaacatt gtagatgctg tgatgcgctt
    13321 tttaatggat aaagataaaa ataaaaaaaa tatagcatct atagcgatta aagttatatt
    13381 tttaagcgtg atagtctttg gatccgtaat gtatttgtta aatattacaa aattatggca
    13441 agatgtttat ggattagaaa aatatatttt tttgtattat atatcctatg tatggaatca
    13501 atttctaata caatttgcta aagggttaga aaaagttaaa gctatgggca tagcaggtat
    13561 tatcagcaca attgtaacaa taattgccaa tattttcttc ttgcttattc tgaaatgggg
    13621 attaattggt tttttcattg caaatatttt atcacagatt atttcagcta cttatctgtc
    13681 agtattagtg agaatatggg aatatatcga ttttgaggct aaagatcctc agctaaagag
    13741 agaaatgcta aattactcga ttccattaat tgcaactgtt gttggatggt ggattaatag
    13801 tacagctgat aggtatgtgg tttcctttat agtaggtgtc tcggcgaatg gcttattgtc
    13861 tgtggcatat aaaatacctc aaataattaa tactttgcag ggaatattta tacaggcctg
    13921 gcaaatttct gcaattaaag aatatggaga taatgagact gcaaaatttt atggaaatac
    13981 atttgttatg attaatttgt taatgtgcat aacctgttca agtcttatca tattaactag
    14041 accgttttca catattcttt atgcgaaaga cttttatgaa gcgtggaaat atgttccatt
    14101 tttattaata gctaatgttt taaattgtgc atcaggtctg ttaggaccta tcttatcagc
    14161 aaaaaaagat tctaaatcca tgatgtggtc tgcaatcata ggggcattag ttaatgtgat
    14221 tatgagtgta gtccttgttt ataagatagg ggttcaaggt gccacaatag ccactgttat
    14281 atgttcatat gtaatatatg ttgttcgtaa acgagctgtg ggaaaagata tttttataaa
    14341 gcattatcat attgttgtgg aatcttgggg attattgttt gttcaggcaa tcatagaaat
    14401 atattttaaa aattatgtaa tagaaattat aattatgatt attttaatta ttataaatat
    14461 cagttatatt aagcagttgt gttatagagg aagaaacatt atttcaatga ttaagagtcg
    14521 agagcacaaa cggtagacga tgttattttt ataaaaagat ataatttatt aatggtcgat
    14581 aattatgaaa ggaaaaatat tcttaaaaaa aagagtatag gatgtcgtat gaaaatagtc
    14641 gctatagtac caatgaagtt aaataacaaa agattgccac aaaaaaacac gaaagctttt
    14701 accaatggaa aaccattgtg ttattatata ctttcaacac ttttaaaagt aaaacagatt
    14761 gataaagtgt atgtctattg tagtaaccca gatattaaag aatttatacc agaaggtgta
    14821 acatttttaa agagatctag ttccttagat caggatacaa cgagtattac acaggtatta
    14881 gccggtttca caaaagaagt agaggctgat gtatatgtaa tgacacacac aacagcacca
    14941 tttattagcg aaaaatctat tgaaaaagga ctagatgccg tgctgacagg agaatatgat
    15001 tctgcatttg ctgcaaaaaa attgcaagat ttcttatgga aagacggtga accatttaat
    15061 tatgaattgg acaatattcc tagaacacag gatttgccgc ctttatacga agaaactagt
    15121 ggattttata tttataatcg caatgtgatg accgagttgc atagaagaat tgggaaaaaa
    15181 ccttgcatag ttgaagtagg tgagattgag tcagttgata ttgatgaagc cgaggatttt
    15241 atgattgctg atgcgattta taatcatctt attttacaag gtagttaaga cagggggaag
    15301 gttaggatga acaatgtaca gattttagat tgcacactta gagatggggg ctattgtaac
    15361 aattgggaat ttggatttga taatacaaaa aaaattattt ctggacttat agaatcagga
    15421 attgagatta tagaatgtgg gtttctcaca aataaaacag tatacaataa aaatgttaca
    15481 aaatatacga ccttagaaga attagcacag ataattcctg aaaaaagaga aggaaaatta
    15541 tttgtagcaa tgatgaacta tggcgaatat aatatagagg atcttccaga ttatgatggt
    15601 tcatctattg atggaattag gattgcgttt cataaaaaaa attgcaatga agcgttgaaa
    15661 ttatgtcgtg agataaagaa aaaaggctac ttagtttttg ttcaagctat ggtatctctc
    15721 aattatacag atgaagagtt tttagcagta ataaaaaatg tgaatcagtt taaaccatac
    15781 gctttttata ttgttgatag ttttggaatg atgaaaggaa aagatttaac tcgtcttttt
    15841 tacatgattg agcataatct agaaaaagat atacagatag gttttcactc acataataac
    15901 atgcagcttg catattctaa tgcacaaaaa cttataaccg tacaaacaaa tcgaagcctg
    15961 attattgatt cttcggtgta cggaatggga agaggagcag gtaatcttaa cacagaacta
    16021 tttgtagaat atttgaatga taatgcagga aaaaaatatc aattaaaacc attattatat
    16081 ataattgatg aaatactaaa tagtttctat cagaagagtt attggggata ttcattacca
    16141 aactatattt cagcagttca taatgctcat cctaattatg caagctatct gtcatataag
    16201 aatactttaa caattgaagc aatgaatgaa atattcgaaa tgatggatga agaaaaaaag
    16261 gtatcttatg ataaacaata tatagaagat ttatactaca aatatatgga aactgggaaa
    16321 atacaggagg aatatatgga agaactgaga aaatttctct cagacaaaaa aattctactg
    16381 attgcaccag gaaaaagttc tgtagatgag gtagaaagaa taaaagagtt tgttaccaaa
    16441 gaagaagtcg taataattag tgtaaatttt gaatattctc atatagatat agattacgta
    16501 tttttaagta atttaagacg ttttcatgaa ttaccgaaag aaagcagaac taaatgtatt
    16561 gttacatcaa atatacaagc atcggatgta tatttacgga cgagttataa aaagttgtta
    16621 tctgaaaggg aagttgtaag agacaatgca ggattaatgg caattagatt tttagccgat
    16681 atgaatgtgg atgaaatatt cttagctgga tttgatggat attcacataa tgaagatgag
    16741 aattatggtg agcaatccat ggaaattata acaaaatttg cattattaga tgcaatgaac
    16801 gtgcaaatga gagatgaatt gtcagaatta gctaaaaaag taaaggtgac atttttaacg
    16861 gatccacgtt gggttcgaat tgataagtag tagtaataat gtttttaaaa ttttgataaa
    16921 gagatagtga gagaataaaa atgggtaaag tcttaaataa aaaagaagaa aaatatttaa
    16981 gggccgatgg ggcgagatga aactctaatt ttgaactttt aagaatcatt tctatattgt
    17041 gtattgttgc ttaccatgct aatttgcgtt taaattataa tgcagacgga attatagtac
    17101 atggttcatt aactgctaac cagattttct tgaattatat tggtatttgg ggtcagctag
    17161 gagttaattg ttttatattt atttcggcat attttttaag tgagaaaaga aacattcact
    17221 ttgaaaatgt tttgggaatt gtattagaat gtatttttta tagtgtatta gtttatggaa
    17281 ttctttgtgc atgtggaata gaaacatttt cattgaacag ctttgtaaaa gaaatattat
    17341 catttgctct aggtagatat tggtttatta catcctattt agcattgtat attctaagtc
    17401 cgtatttaaa tatcatgctt aacaatctgg aaaaacttca gttgcaaaaa ataagtatgt
    17461 tattgtttat aggtgtatgc tgttatcgta tgttatttaa aacatctttg tttggggatt
    17521 gtgctgtttt aattgcacta tatgtcatat gttatacact gaaaaaaacg ataataatat
    17581 ttttgagaga cacagaatca taggaacaat tgtatgtata acagttatac cacttctcat
    17641 atccatagga atacttttca aatttatacc aaatgcaatg gttaaccaga tggttcatat
    17701 tggttctcca ttgatggtta tagcaagtat atttttattt tatgtattta aaaatttttc
    17761 attttacagt aagtatataa ataaacttgc gagaaccagt ttaggggtat atattttaca
    17821 tgaaaatcat tatttcattg atggtatgaa gtctagaggc ttaatatggg atgggatatt
    17881 tggattaggc aaaaggtttt tagataggga tttttggatt tggtttataa ttgcagttgt
    17941 agttgtgtgc ataatttgca tggctatcga tgttgtaaga gaaaaatttg aaagacagat
    18001 ttttaaaatt aagagtgtaa agaacctgtg taaacttatg aatcatgtta taaatatata
    18061 cccataatta ataaatgagg agaaaagaag tgaaagttat atcattcgga gaattattat
    18121 tgagacttgc agcacccggt tattcaaagt tatttcaaag agatagtttg gagactacat
    18181 tctgcggagg ggaagctaat gtagcggtat ccttagcaat atttggatat caatcagaat
    18241 ttataacaaa gcttccggat tcagatgttg gatatgcggc agctaatgct ttgcgttatt
    18301 ttggagtaaa tgttgataat attatatttg gagagggacg catgggactg tattatttag
    18361 aaaaaggagc agctcagaga ccatctaaag taatttatga cagaaaattt tctgcaattt
    18421 cgttagctga tagtgatgaa tttaagtgga aggagatcct aaacgatgca aattggtttc
    18481 attggacagg gataaatcca gcattgtctg agaatctctt tgagacgtcc ttagatgcat
    18541 gtcaggctgc caaaaaaaga ggtataacaa tttcttgtga tttaaattat cgatccaagc
    18601 tttggacatc agaggaagca aaagaaaaga tggaacagat actaccattg gttgatattt
    18661 gtatttgtaa tgaagaagat gcagaaaaag tgctaggaat taaagctgag catacaaata
    18721 ttaatagtgg agaattaaat aaagatggct atgtgcaggt ggctagggag attcacaaga
    18781 agtttggatg taggtatgta gctacaactc tgcgtagaag ttattcggca agtagaaatg
    18841 gttggagtgc aatgttatac gaagcagaaa aagataaagc gtatttttcc acagaataca
    18901 acatacaaat tgttgatcgc gttggtggtg gagacagctt ttctgctggt ataatatatg
    18961 cgttatgtga aaatatgtcc ggacaaaagg ctattgaatt tgcgactgca tctagttgtt
    19021 taaaacatac tatagaaggt gattttaatc gaactacaat tgaggatgta tataatttaa
    19081 tgaatagcgg tggaaatggt cgcgtgttgc gttagtcaac atataaagat aaatcacagt
    19141 aatttttatg gtgattatct gtcttagtag tgattagaat aacgtgaaaa taaattgttg
    19201 agcgtttata ctgttttgaa aaataaattt aacatattga aaaatacaaa tatatacgta
    19261 tattataaag cgccgattgt aaaattaagc tagacaacta aaacagtcat ttgtgctaca
    19321 ctcaaaatag ttccgaccaa agaattataa ggagtgagtg caaataacct atacctatct
    19381 tacaacggat gaacttgtaa taatagagtc ttatttgaaa attaaccaat ctgttgctaa
    19441 aactgcgcat tgcttgaatc gttcaagtca aacgatccat aaagtatatc tgttcttcaa
    19501 gcaaagaaaa tcagccttag aata
//
LOCUS       DACGLH010000049        19476 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.053, whole genome shotgun sequence.
ACCESSION   DACGLH010000049 DACGLH010000000
VERSION     DACGLH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.053"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IXT21_001896"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IXT21_001896"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ4869282.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IXT21_001897"
     CDS             complement(1174..1869)
                     /locus_tag="IXT21_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ4869283.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IXT21_001898"
     CDS             complement(1882..2598)
                     /locus_tag="IXT21_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ4869284.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IXT21_001899"
     CDS             2928..3533
                     /locus_tag="IXT21_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ4869285.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IXT21_001900"
     CDS             3858..4181
                     /locus_tag="IXT21_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ4869286.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IXT21_001901"
     CDS             4363..5733
                     /locus_tag="IXT21_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ4869287.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IXT21_001902"
     CDS             complement(5863..7164)
                     /locus_tag="IXT21_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ4869288.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IXT21_001903"
     CDS             complement(7394..9385)
                     /locus_tag="IXT21_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ4869289.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IXT21_001904"
     CDS             complement(9529..9795)
                     /locus_tag="IXT21_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4869290.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IXT21_001905"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IXT21_001905"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ4869291.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IXT21_001906"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IXT21_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ4869292.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IXT21_001907"
     CDS             13139..13702
                     /locus_tag="IXT21_001907"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ4869293.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IXT21_001908"
     CDS             complement(13955..14476)
                     /locus_tag="IXT21_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ4869294.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IXT21_001909"
     CDS             complement(14479..15321)
                     /locus_tag="IXT21_001909"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ4869295.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IXT21_001910"
     CDS             complement(15314..16039)
                     /locus_tag="IXT21_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ4869296.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IXT21_001911"
     CDS             16215..16511
                     /locus_tag="IXT21_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869297.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="IXT21_001912"
     CDS             17079..18671
                     /locus_tag="IXT21_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4869298.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="IXT21_001913"
     CDS             18684..19424
                     /locus_tag="IXT21_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869299.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5566 a         3999 c         3698 g         6213 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttt
//
LOCUS       DACGLH010000050        19452 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.054, whole genome shotgun sequence.
ACCESSION   DACGLH010000050 DACGLH010000000
VERSION     DACGLH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.054"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            133..3660
                     /locus_tag="IXT21_001914"
     CDS             133..3660
                     /locus_tag="IXT21_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ4869300.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IXT21_001915"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IXT21_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ4869301.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IXT21_001916"
     CDS             7448..7789
                     /locus_tag="IXT21_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4869302.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IXT21_001917"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IXT21_001917"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ4869303.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IXT21_001918"
     CDS             9146..11566
                     /locus_tag="IXT21_001918"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ4869304.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IXT21_001919"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IXT21_001919"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ4869305.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IXT21_001920"
     CDS             13000..13737
                     /locus_tag="IXT21_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869306.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IXT21_001921"
     CDS             13751..14566
                     /locus_tag="IXT21_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ4869307.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IXT21_001922"
     CDS             14579..15226
                     /locus_tag="IXT21_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4869308.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IXT21_001923"
     CDS             15242..15898
                     /locus_tag="IXT21_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4869309.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IXT21_001924"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IXT21_001924"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ4869310.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IXT21_001925"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IXT21_001925"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ4869311.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IXT21_001926"
     CDS             18240..18758
                     /locus_tag="IXT21_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ4869312.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IXT21_001927"
     CDS             18865..19365
                     /locus_tag="IXT21_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4869313.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4177 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DACGLH010000051        19363 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.055, whole genome shotgun sequence.
ACCESSION   DACGLH010000051 DACGLH010000000
VERSION     DACGLH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.055"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(210..1661)
                     /gene="cls"
                     /locus_tag="IXT21_001928"
     CDS             complement(210..1661)
                     /gene="cls"
                     /locus_tag="IXT21_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ4869314.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            2002..4668
                     /locus_tag="IXT21_001929"
     CDS             2002..4668
                     /locus_tag="IXT21_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4869315.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5032..5784
                     /locus_tag="IXT21_001930"
     CDS             5032..5784
                     /locus_tag="IXT21_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4869316.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5806..6960)
                     /locus_tag="IXT21_001931"
     CDS             complement(5806..6960)
                     /locus_tag="IXT21_001931"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ4869317.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7145..9586
                     /locus_tag="IXT21_001932"
     CDS             7145..9586
                     /locus_tag="IXT21_001932"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ4869318.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9916..10620)
                     /locus_tag="IXT21_001933"
     CDS             complement(9916..10620)
                     /locus_tag="IXT21_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869319.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10807..11052)
                     /locus_tag="IXT21_001934"
     CDS             complement(10807..11052)
                     /locus_tag="IXT21_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869320.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11069..11920)
                     /locus_tag="IXT21_001935"
     CDS             complement(11069..11920)
                     /locus_tag="IXT21_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ4869321.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12086..13090
                     /locus_tag="IXT21_001936"
     CDS             12086..13090
                     /locus_tag="IXT21_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ4869322.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13331..13687)
                     /locus_tag="IXT21_001937"
     CDS             complement(13331..13687)
                     /locus_tag="IXT21_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4869323.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14055..15242
                     /gene="tuf"
                     /locus_tag="IXT21_001938"
     CDS             14055..15242
                     /gene="tuf"
                     /locus_tag="IXT21_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ4869324.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15430..18021)
                     /gene="mprF"
                     /locus_tag="IXT21_001939"
     CDS             complement(15430..18021)
                     /gene="mprF"
                     /locus_tag="IXT21_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ4869325.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18047..18766)
                     /locus_tag="IXT21_001940"
     CDS             complement(18047..18766)
                     /locus_tag="IXT21_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4869326.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            18975..>19363
                     /locus_tag="IXT21_001941"
     CDS             18975..>19363
                     /locus_tag="IXT21_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ4869327.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGK"
BASE COUNT         6262 a         3510 c         3926 g         5665 t
ORIGIN      
        1 cgtttaagaa agaggtcgtg agcctgccgt ccagctccga gtcacaagga acaagttcaa
       61 aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt ggcaggagaa
      121 caccgtttgt tcggaagaag ggactgtgac aagatttttg tcacagtccc tttaatctta
      181 gtatattttt ctctacttga ataaaaaaat tataggattg gagaaaataa tcgagaaatt
      241 tgttgcttga ataatagcca gttagacatt tccttgactg tttcagttgt catggatgaa
      301 caatgtgtct catcttcttt gaactgagta gcaagttctt ctaaaaactc aggatcgtaa
      361 atgacagcac ttgtttcaaa attcaatcgg tagctacgaa tatcttgatt tgctgaacct
      421 accatgcaga tttcatcatc cattatcaat gtttttgcat ggaggaagcc gttctcatag
      481 acaaggattt gtacattttc cttgatcagc tgccgagcat aatattgtgt tgctcgataa
      541 ataaaaggat gatccggttt gttgggaatc atgattttca catctacacc agaggcagtt
      601 gcgattttta aagcagcaat gacactttca tcaggaacaa ggtaaggtgt ctgtatccaa
      661 acacgttttt tagcagaagt aatcaatttg ataaatgaaa gcttgatttg ttccctttcg
      721 ttattcggtc cgcttgaaac aagctggatg gatgtatcag caagcttaga ttcatctcga
      781 tctgcttttt taaagaaata attcaaatga tagccgacct ttttttcttc aggtaccgag
      841 acgttccagt ctgttaagaa gcgaagctgg agcaaagaag aagcggcacc gaaaatccgt
      901 atatgcgtat cgcgccaata gccgaatttt tttgttgtat ttacatattg attggcaata
      961 ttgaaaccac cggtataact aatctttcca tcgatcacaa cgatttttcg gtgatcatgg
     1021 taattcaatc ggaaacgaag taatgccctt tgtgaggtaa caaatgtatg gacatgtcca
     1081 ccatttttga ttagacgatt gaaatctttt gcttttgtgc catgagagcc aaatgcatca
     1141 tataatattc gaacttccac gccttctgcg gctttttctt ctaaaacatg taagacttgc
     1201 tggccgatat tatctgtcac aaatgcgtaa tattcgatat ggatcgaatg tttggctttt
     1261 tttagatctt gaagcagtga atccaatttc tcttgtccgt ctgtgagaag agtgacagaa
     1321 ttcattcttg tcagcggcat acgatttaat gaagagaaga agtcaacaaa ttgctgtttg
     1381 tctttatcgc ccatgtcagg atcgtaatgt tcaatggtat ctcctctaaa agactgaaag
     1441 ttttctaatt ctttcaagtc actttgtcgg agatagaatt ttttcttatc cgttaatcca
     1501 cgtccgaaaa ataaatataa tacaaagccc accccaggaa gggcaaacaa tactaagagc
     1561 catgcccaaa tggctgctac atctcggggt ttgatcaaaa tagccaccaa agcaataagt
     1621 gcatttaata gataaagggc ggttataata ctagatacca caaaaatgtt cctccttaca
     1681 tacaataaca ttagtatagc atgatagaag aaagaagcct accaaggtga atatactttt
     1741 cactcctttc atgaaaatgt aggtaaatcg ggctaaagta gttgcttttt gtaggttaat
     1801 aaggtaggat attagtacag ttgaatatct ccttagggga gtaaccggca acgtttgttg
     1861 cgataatatc aacagtaagt taaccaaatg gttttctggt attgtatgaa atggtgagac
     1921 ctaagacaaa tgcgtatttg tcttaggtct cttttttttc agacaggact agggccaaga
     1981 tcaaaaaatg ggggatgaaa tttggaaaac aaggaacaag cacaacaagc ggtctataaa
     2041 gaaacgatcg actcgttgat gcagcaaacg cagagctcaa ccgatggatt gagcaccacg
     2101 caagcgaagg aaaaattaga aagagatggc ttgaaccagc taaaagaaaa gccgaagaag
     2161 ccaacttgga aattgttctt agaaactttc aaagatgcaa tggttatcgt attgctgatt
     2221 gtcgcactga ttcagatggt catgggtgac tacatcgaat tcttagttat ttttgcagta
     2281 ttgatgttga attctataat cagtgttgtc caaacaaaaa aagcagaagg ttctttagaa
     2341 gcgttgaaaa acctttcagc gcccgatgct cgagtgattc gtgatggtaa ggaaaaaacg
     2401 attccggcaa acgaacttgt ggtaggagat atcgtgttgt tagacgcagg cgattatgta
     2461 cctgctgatg gacgtttact agatgcggga tcattaaaag tagatgaagg tatgctgaca
     2521 ggagagtcga cttctgcaga aaaagaagta acggatatca caagtacggt accaatcgga
     2581 gatcgtacaa atatggtatt cagtggaacg atcgtaactt acggacgcgg taaattctta
     2641 gtgacttcaa ctgggaataa tacggagatc ggacaagttg ctggcctttt ggaatcaaca
     2701 actgagcaag tcactccgtt acaacgaggt ctggataaat tcagtaaaaa actaagtttg
     2761 gctattttgg cattgtcttt attgatttta ggtatccaac tgtttcgtat ttatcttgga
     2821 gaaggtacag gagatatgac tgcagatatc gtcagcgcag tgatgtttgc cgtagccgta
     2881 gccgtagctg ctattccaga agcattacag tctatcgtaa caatcgtgtt atctttggga
     2941 acgaataaaa tggcaaaacg tcatgccatt attcggaagc ttccagcagt tgaaacactt
     3001 ggttcgacaa gtatcatctg tacagataag actggtacat tgacacaaaa caaaatgaca
     3061 gttgttgact actttttagg taatggcgat actggggatt tcacaaatga cccttcaaaa
     3121 tggacagctg acgaacgtcg cttgatcgaa atcagtgttt tagcgaatga tgcatcgatc
     3181 agtgaagacg gtactaaatt gggcgaccct acggaagtag cgtttatcga ctttagtgaa
     3241 aaattaaacc agccttatca ggaaatccga aacaactacc caagacaagc agaacttcct
     3301 tttgactcag atcgaaaatt aatgtctact ggacacacaa ttgataacaa aaattacttg
     3361 tttgttaaag gcggaccaga tatcgtcttc caacgcagta aaaaagtatt gctaaatggt
     3421 gaagtcgttc cgatgacgga tgaattgatg aaaaaattcc aagagcaaaa tgaagctttt
     3481 tcaacacgcg cattacgtgt actagctttt gcttaccgtc cacttgataa ttttgattta
     3541 tcatttgaag atgaagacga tttgatcctt gttggtttgc ttgctatgat cgatcctcca
     3601 cgtgctgaag tgactcaggc ggtaacagaa gcaagaagtg caggaatcaa aacaatcatg
     3661 attactggtg accacaaaac aacagctgtt gctatcgcaa aacaaatcgg gatttttgaa
     3721 gaaggaaacc tggctttgac cgggacggaa ctagatgaat tgacagaaga tcaactaaac
     3781 gaagttttag aaaaagtcac tgtttatgca cgtgtttcac ctgagaataa aattcgtatc
     3841 gttcgtgctt ggcaaaataa aggtcatatc tcagctatga caggtgatgg tgtaaatgac
     3901 gcaccagcat tgaaacaagc agacatcgga attgctatgg gaacgggtac agatgtggca
     3961 aaagatgcag cagcgatggt attgacggat gataactttg cttctattgt cagcgcagta
     4021 gaagtaggac gtaatgtgtt tgataatatc aagaaagcta tttcttatct atttgcagga
     4081 aatttaggtg cgatcattgc gatcatcttt gccttagttg ctgactggtc caatccattt
     4141 acagcattac aactgttgtt catcaattta gtcaatgatt cactgccagc aattgcatta
     4201 ggtacagaaa aagctgaacc tgctacgatg aaccgcccgc caagagatcc aaattcagga
     4261 atctttacgg acaaaacatt gatttctgta acgtatcgtg gtgtgctgat tgggattgct
     4321 gttatctttg ctcaatggct tggcatgcag cattcacctg aattcggtgt agccatgtca
     4381 ttttcaacat tgatttggtg tcggacattg caaacattcc cagcacgttc aaacacacaa
     4441 acagctatcc aagcagggtt cttctctaac agaagtgtct tactagctgt agttgtatgt
     4501 agcgcgcttt actgcttaac aatgatccca ggactgaaag aagtcttctc tatcccatca
     4561 gcatttggat tgacagaagt ctttacatgt atgggaatcg cacttgtgac aattgttttg
     4621 atggaaatca ctaaattgat cattgtacga gtacaaggaa ataaataaaa cgaatcaacg
     4681 aggttgtgag cctgccgttt aattctgagt cacaaggtgc aatttcaaca aacagcttct
     4741 catgtttgtt gaatattgtg acttgtgacc gaagagttgg caggagaaca ccgtttaagg
     4801 aagaagttgt aaaatcagca ttttgagcta atactgaggg tcatctgatt ggacactgtt
     4861 tattcggaaa gagggacttt gacacgactt tggtcacagt cccttttttt atttatgata
     4921 gacggaaagc caatcatttt attattcatc aaaagagata gaatagataa aagaggagaa
     4981 tgatttattc cgaaaagtga aaacatataa caaaaaatta gaggaaatca aatgaatgaa
     5041 aaagaatggg atgaatttgc tgaagaatat tatgaaatcc agcaagaatc gcaagtaact
     5101 attgctgaag atgtaaaaaa ttatctgaaa acggagaaag tcctgcctgc tgaaacagca
     5161 gcagacgttg ctggaggatc tggcagattt ctttccttag caaaagaaat taatcgctat
     5221 gatttgatcg atttttcttc aggaatgcta gaaaatgcaa aaaaagaagc agaaaaaaat
     5281 gagttgccaa atgtttcctt tatcaaacag tcgttagaag aatttatcca atcaaatgag
     5341 acatatgaaa tggtttttac agcagcaaat ccagcattgg tacatcttat tcaattagaa
     5401 aaattgatga aaaagtcaag caaatggtgc gtgataatca gagtagtgga atcaatcgat
     5461 gatgtttttt atcctatcga aaagcaacta ggaatgatag aagaagatcc taatacatcg
     5521 ccagttgtaa tggatcaatt tgaaacatac ttaattgatg aaggattcaa agtgaagaaa
     5581 aaagatttta cttattttac acaggaatca attactgaag aattgatgaa gctatactat
     5641 gacgcgtacg caaaagatcc tgttgtgacc aggtacattc gcaaactatt cagtgaaaaa
     5701 cgagaaatcc ttagtacgac taagctgacc tatagactgc tcctttggtc cgcagataaa
     5761 aaaagaacct ttcacgactc gtgaagggtt ctttttatga ttttattata ttttgtagta
     5821 acgaatatca ttttcgatct ttgtaatcga aaaaggtaca tctgaagtat attctgcaca
     5881 ttctccgtgt tcaggaagcg tctcactgaa aatcgtttca tattcgtcaa cagtgatccg
     5941 tgttcgagaa tctagcatct cttgatgaga ttgtgcgaag agatattctt gatagttttc
     6001 ttccagataa cctgtaaaaa attcacttac tgcaccagaa ccgtagctga atagaccgat
     6061 gcgatctcct ggctgtagtg atttcgaatt ttccagtaga gaagttagcc ccaggtataa
     6121 tgaaccagtg taaagattac cgattcgtcg gctgtaacgg atgctttctt catagcgagc
     6181 cataagacgt tcctgatttt cttcgtctgt ttggtctaat acgctttgca atgccttttt
     6241 tcccatctta gtatacggaa tatggaaagc aatcgcctga taatcttcga gtcctcgacc
     6301 cgacaattct ttatgtcgat tccaaacttt ttggaatgat tcgatatacg tagaattaga
     6361 taaaggacca tcaacaacag gaaattcgct ataatctgga cgccagaaat catagatatc
     6421 ttctgtcaga aatacgctgt cgtcttcaat cgataaaata cgcgggtttt gagtgatcat
     6481 catcgcaaca gcaccgacac cttgcgtcac ttcaccaccg cttgccaagc cgtaacgagc
     6541 aatatcactt gctatgacta gtacttttcg ttctggatga tttttgacat attctttcgc
     6601 catgtgcaaa gctgcggtgc caccgtagca agcctctttt acttcaaaag aacgagcaaa
     6661 agattggatt ttcagtaaat ggtggataat taccgcactc gcttttgaat gatcaatccc
     6721 ggattctgtt ccgacgatga ccatgtcgat tagctcacgg tctttttctg ttacgatttt
     6781 gctggcagca tttgctccta gtgtaatgat gtcttcattt gcacgattga ctgccatttg
     6841 gtcttgacca atccctatat ggtattttgc aggatcatcc ccacggcttt ctgccaactc
     6901 tgtcatatct aaatataaat taggaataaa aaaggaaaga cgatcaatcc ctattttcat
     6961 ttcaacacct ctttacttat gataaaaaac ttactttact aaggtatcat aaaaagatta
     7021 aaagaaatat aaaaaagaga atatttaagg attgttaaaa tttaatctta gaaaaaaata
     7081 actggaacaa gagctatttg tttttacagc aaagaatggt atatttttgt aggaggtggc
     7141 ttacttgaaa gaagtcgtta tgatagatgc tgcaagaacg ccgataggaa agtatcgcgg
     7201 aagtttaagt ccatttactg cagttgaatt aggcacactt gtgactaaag gattattaga
     7261 taaaacaaaa ctgaaaaaag ataaaatcga tcaagtaata tttggaaatg tcctgcaagc
     7321 tggtaatggt caaaatgttg ccagacagat tgctttgaac agcggtctcc ctgtagatgt
     7381 cccagcaatg acgatcaatg aagtttgcgg atctggtatg aaagcagtga tcttagcacg
     7441 ccagctgatc caattaggtg aagcagaact tgtgatagcc ggcggaacag agagtatgtc
     7501 tcaagcacct atgctgaaac agtatcagtc agaaacaaat gaatatggtg aaccaatttc
     7561 cagtatggtc aacgacggat tgactgacgc attttcaaat gcacatatgg gattaaccgc
     7621 agagaatgtt gcaacacaat tttctgtgag cagagaagag caggatcgct atgccttgtc
     7681 gtcccagttg aaagcagcac atgctgtcga agccggtgta ttttctgagg agatcatccc
     7741 agtcaagatt tctgatgaag acgtgttatc tgaggatgaa gcagttcgtg gaaatagtac
     7801 attggaaaaa ctgggcacgt tacgtacagt attctcagaa gaaggaactg taacagcagg
     7861 aaatgcttcc ccgttgaatg acggtgcctc tgtggtgatc cttgcatcca aagaatacgc
     7921 agaaaataat aatctgcctt atttagcaac catcaaagaa gtagcggagg tcggtattga
     7981 cccaagtatc atgggaatcg ctccaatcaa agccattcaa aaattgacag atagatcagg
     8041 aatgaattta tcaacaatcg atctttttga gatcaatgaa gcatttgcag cttcttctat
     8101 cgtagttagc caagaactgc agttagatga agaaaaggtc aatatatatg gaggagcaat
     8161 cgcattaggc catccaattg gtgcaagcgg tgcacgtatc ttgactacat tagcttacgg
     8221 attgcttcgt gaacagaaga gatacggtat tgcttcatta tgtattggtg gaggtttagg
     8281 cttggcagtc ttgttagaag cgaatatgga gcaaacacat aaggatgtgc aaaaaaaaaa
     8341 gttttatcag cttacgccat ctgaacggcg cagtcaatta atagaaaaaa atgtattgac
     8401 acaagaaact gcgttgattt ttcaggaaca aacactttct gaagagctct ccgatcacat
     8461 gatcgaaaac caagtgagtg aagtggaaat cccaatgggg atcgcgcaga attttcaaat
     8521 caatggcaag aaaaaatgga ttccgatggc aacagaggaa ccatctgtca ttgcagctgc
     8581 aagtaatggt gcaaaaatct gtggaaatat ctgcgcagaa acacctcagc ggctgatgcg
     8641 agggcaaatc gttctatcag gaaaatcgga gtatcaagca gtgatcaatg cagttaatca
     8701 tcgaaaagaa gagctgattc tttgtgcaaa tgaaagttat ccttcaatcg tgaaacgcgg
     8761 gggcggtgtt caagatatct ctactcggga attcatgggg agtttccatg catacttatc
     8821 tattgatttt ttagtggatg taaaagatgc aatgggcgct aacatgatca actcgattct
     8881 tgagagtgtg gcaaacaagt tacgggagtg gttccctgaa gaagagattc tattcagtat
     8941 tttaagtaat tttgctacag aatcattggc ttctgcctgt tgtgaaatcc cattcgaacg
     9001 cttagggaga aacaaagaaa tcggtgaaca aattgccaaa aaaatccaac aggcagggga
     9061 atatgcgaag cttgaccctt accgtgcagc aacgcacaac aaagggatca tgaatggtat
     9121 agaagctgta gtggcagcta caggaaatga tacacgtgcg gttagcgctt cgatccacgc
     9181 ctacgcggca agaaatggat tataccaagg attaacagac tggcagatca aaggggataa
     9241 actagttgga aaactaacgg tgcctttggc tgttgccaca gtaggcggag cttccaatat
     9301 cctgccaaaa gcaaaagcat ctttagctat gttagatatt gattcagcca aggaactagc
     9361 acaagtaatt gctgcagtag ggttagctca aaacctggct gctttgcgtg cattagttac
     9421 agaaggaatc caaaaaggcc acatgggact tcaagctcgt tcattagcga tctcaattgg
     9481 ggctattggt gaggaaatcg agcaagttgc gaaaaaatta cgtgaagcag aaaagatgaa
     9541 ccagcagaca gctatacaaa tattggaaaa aatccgggaa aaataaaaaa gcaatatctt
     9601 tagaagaata aagcaagatt tacagttatt ttatttactg cagatcttgc ttttttctct
     9661 atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat cttaagaaat
     9721 caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt aggatcatag
     9781 gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga atttcttttt
     9841 acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc cagcctttta
     9901 tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt cgatcaaagg
     9961 aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa cgaactcatt
    10021 ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt tttcattaat
    10081 aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa cggcttttga
    10141 aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt cagttgtgtc
    10201 cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg taactggtgt
    10261 cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc agtaagacac
    10321 gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc ctgaacgaat
    10381 tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg tatcttcatt
    10441 tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa gcatgacttg
    10501 taacggcaaa ccataacgtc gcaagctctc ttcttctaga gaacggaata attgtcctct
    10561 tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt ttttcttcat
    10621 atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca aatgaaaagg
    10681 agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc gtattttccc
    10741 gttttttgaa agaaaaataa aaaatggaac ggattatgta tatatacata atccgttcca
    10801 ttctttctat tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat aaatgacttc
    10861 atgttcgtgc cagggtttga ccattagcga atgatcgaac cgttttttca gttcttcgat
    10921 gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg attttctctg catcgatcaa
    10981 aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc gagagagaat tcaagcgttc
    11041 gattggatcc ataaaatcac ctttcgactt aaaattgggt agtatctggg tcttttgctt
    11101 tgccgactac accatctaac tgatcgattt gtttcatttc ttcttctgtt aattcaaaat
    11161 caaataattc agtattttct ttgattctgc ttggtgtcac tgacttagga agaggaagga
    11221 attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca tgtgcttcag
    11281 cgatttgttt catttcaggc acatcgaaga ttttaccagt gcctaatgga ctatatgctt
    11341 cgaggatcat atcatgtttt tttgcatact caactacagt tgtctgtaat tcgcctggtg
    11401 caaggaaaac ttgattgacc atcggcataa tttttgctgt ttttgctagt gtatctagat
    11461 ggtggggtaa gaaattgctc acaccgattg ctttgatttt acctgcttca tacagttctt
    11521 caaatgctcg ccatgtatct gcatttgctt gttcccaatt atcacgaaaa gcaacagggt
    11581 ttggccagtg gatcaaaaac agatctaaat aatctgtttg gagcttcttc aatgattctt
    11641 caaaagaaga catcacaagt tcatagctgt ggtttgcatt ccataatttt gttgtcaaga
    11701 atatctcttc tctaggaatt ccactttcct taatcgcttg gccgacgctt tcttcattct
    11761 tgtatccttg ggcagtatcg atatggcgat aacctgcagc cagcgcttct ttaacagagg
    11821 aaacagcgac atccccatca ggtgtttgcc atgtgccgaa tcctactacc gggatttcgt
    11881 aaccatttga tagacgataa gtgcttgtta atgaattcat actttcagct ccttttttat
    11941 tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga atccattttg tttctactct
    12001 tgaaattttt gtttaaaaga aaatgatttt gattctggta tttttgattt tttttccgta
    12061 aactaagaca gaagggaatg agaaaatgga tatctattta aaaaaagcaa ttctgcatat
    12121 tattgacaga gaaactggtt ctccagtctt ttcacaaaaa gagctggatc tgacaaaaga
    12181 atatattcgt gattttcttc aaaagaagat ccaaaaaatc tcttctgctc aaacaaaaac
    12241 tggacaacta gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc aagaaaattt
    12301 tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat atctaccaag aaagtgaaga
    12361 agcacctagt gcagatgtat ttgttgtctt gtatgaatta gatacagtga tgtacttatc
    12421 tttattgaaa gtcaattatc gtgaggctta cactcacttt gttgaagcag acgaagcagg
    12481 gatcgacaac cgcttgattt tgaatcgagc aattctaggg gcgaaaacac aaaaagcaga
    12541 tgaagcattc gctgtgaatt tgactgattg gacctacgaa ttaattgaaa aaaaatatga
    12601 gttttctggc aaaaaagagc tttatttttc tggaagagtg atcgaaagtg ttcctgcacc
    12661 ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg gaaaaaaatt
    12721 tgagacagaa gaatttgata tcatggctga tttgaaagaa gctgtctatg atacgataga
    12781 agaaaaaggt cggttggatc atgagatgat tgcggaaaaa gtcttcaagg aaaacatcac
    12841 ggcaaaaatg gcttttaaag aagaagtaca agagcaagga tttgtcccag aagcacctcc
    12901 tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat tcaagctatc
    12961 aaacgggatc gaactgatcg taccagtaga tgtatatcgc aacccagatt tgattgaatt
    13021 tgtgaataat ccggatggta cgatatccgt gatgatcaaa aatgtggatg aagtattgaa
    13081 tcgtttataa aaatgactag aatattaggt gttcaagtac cagtgatcag ttaaatgaat
    13141 tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa aaagagcgtg agacaaaagt
    13201 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc
    13261 ttcttatttc ttggagttaa acgcttctgt cccagcctct ttttatctaa taaaatttta
    13321 agaccattta ctaattatcg attttttctg ccgcattttt aggcacttca gaagccttta
    13381 tttcttccca gcttaataca cctttacgag gattgacttt gagtttaaga tacgcattca
    13441 tctttaatgg atgttctctt tcttcgcgaa gcgtcacctg ttttttttca ccatctttat
    13501 tataagccgc ttgcttgtag gtataagtcg tgaatttctc tccgttatct gcttgggtag
    13561 ctgacatttc gccattcgtc gtgatttttg tataataagc ttcgccgccg taaaaataat
    13621 gataagctgc aaaacttccg ccgagaaata tgcctaaaac gacaagtacg ccaattaatt
    13681 ttttcatgta cttcacctct ttccttatta ttaacgaaat agaagatttc gtctattgaa
    13741 agttgttggc aacagctgaa ttttaaactt ttcctcattt tgttcttatt gcttcaaaag
    13801 aaagttaagg actatcttga agtttttctc aaaaaacaca tttttttatt tataaaaggt
    13861 acttttttcg cgtataagac agacgagtgc tcttagttga ctttgaccaa tattgacgta
    13921 taataagctt agttcgaatc ttacaaaaag taagtcggac gtattctttt tgatttagtc
    13981 aaaaaaagac tataataaaa aggtgagagc tgaaattttt cagtgatcta tattatttag
    14041 gaggtttgta aattatggca aaagaacatt atgacagaag taaaccacac gttaacattg
    14101 gtacgattgg acacgttgac catgggaaaa ctactttaac tgctgcgatc actacagtat
    14161 taggcaaaaa aggcctagca aatcctcaag actatgctag tattgatgca gctccagaag
    14221 aacgtgaacg tgggattacg atcaataccg cacacgtaga atatgaaaca gaaaaacgcc
    14281 actacgcgca tattgatgca ccaggacacg cggactatgt gaaaaacatg attacagggg
    14341 cagcacaaat ggatggtgca atcttagttg tttctgcaac tgacggtccg atgcctcaaa
    14401 cacgtgaaca cattttattg tcacgccaag ttggtgtaaa atacctgatt gttttcttga
    14461 acaaagttga tttagtcgat gatgaagaat tgatcgattt ggtagaaatg gaagttcgcg
    14521 agttattgag cgaatatggt ttcccaggcg atgacactcc tgtgatcaaa ggttccgcat
    14581 taaaagcatt gcaaggcgat ccagatgctg aagctgctat tatggaattg atggatacag
    14641 tagatgaata tatcccaaca ccagaacgtg atacagataa accattacta ttgccagtgg
    14701 aagacgtctt ctcaattaca ggtcgaggaa ctgttgcctc aggtcgtatt gatcgtggtg
    14761 ctgttcgtgt cggtgatgag gtagagatcg tagggatcaa acctgaaaca caaaaagcag
    14821 ttgtaacagg tgtagaaatg ttccgtaaaa cgttagatta cggggaagct ggggataacg
    14881 taggcgtgtt gttacggggg atccaacgtg acgatatcga acgtggacaa gtacttgcta
    14941 aaccaggttc cattactcca catacaaaat tcaaagcaga agtgtacgtg ttgacaaaag
    15001 aagaaggtgg acgtcatact ccattcttca acaactatcg tccacagttc tacttccgca
    15061 caactgatgt tacaggaaca atcacattgc cagaagatac agaaatggtc atgcctggtg
    15121 acaacgtgac gattgatgtt gacttgatcc atccaatcgc tgttgaaaac ggaacgacat
    15181 tctcaattcg tgaaggcgga cggacagtag gttcaggtat cgttacagag atcgaagcat
    15241 aagaaacgcg actcgaatat tttggtaatg aaaaatgttt aaaataaaag agcggtccaa
    15301 acaggatttc tgtttggacc gctctttgtt cttcaattgt ataagaagag attgtagaag
    15361 catgaacttt cgttttactt cagtctcttt tattcatcat gagagtgttc atctatcggt
    15421 atgcagtttt taatactttc ttcgtatcca ttttttcaaa ccacttattc taccttgtgt
    15481 ctgctcaact ggcgcattat caataatcag caaagcaatc ataacatatg caatccaact
    15541 atctctagaa tatagagtat aacgtgagac ccagtttgta gcgtacttgc ttttatattc
    15601 tcttaatcca tggaaagaat aaaaatgaga accaaactcg taaaccaagt atgcaatcct
    15661 ttcctgtaaa aaactctttc tggaagttcc gacatttgaa agaggggcca tccctaaatt
    15721 gaaccattgg atatgttctt ctttcatata ggcaaacaaa tgcaagaaca agaaatccat
    15781 actgccagaa ggtgcttttt tagaatcata tcgcatcaga tcaatcgttc ctactttatt
    15841 gttcgtgtaa gtaggcatga tcgttgcaaa agcgacaatt tcttcttggg tatttttgac
    15901 aactgcaata ggcgctcttt gtagataatc ttcagagaaa aagcctaaag aaaacccttt
    15961 ttcctttcgt gaacccaacc aattatcaga gatatttttg aaagtactca tttgctcagt
    16021 agaaaatggt ggttgtaaga catcaaatgt aaatccttct cgttcgatcc gattcaagac
    16081 agcacgagtg cccttcattt ttttccctga agttgtaaaa ctattcaggt ctacataagc
    16141 ttcttccccc atcttaatga aatcgtagcc gaattcatgc aaaatcataa caatctcttc
    16201 gcttgtttcg tagaaaactg gtaaatagca tagtcgatct gtttcttcga taaaagcttc
    16261 gattgcttct ggaaaatctt ctttcttacc agaaggatca cccattacaa tacatttatt
    16321 attgaagcaa gcgaattgga gaagaacagt tggttcaccg tctttttcat aggcaaacat
    16381 ccgcttatct tttaagaaga tcaattgact atcggagttt cctccataag ttgtaaggat
    16441 cttcagggcc ttttcttcat taaaggcttc gccaatctgt ttcttttctc cttgaaggaa
    16501 atgaacaaat agaacgatca tgaaactaac agcgatgatg gccaacaatc ccgaaaacca
    16561 aatcttttca gaaggaaata agaaaaagga aataaaatgg tgacgccgat gagggaaatc
    16621 tggcagattg tataccccaa taacgatgta aagcaaggac agtgcaccaa taatgattcc
    16681 atcgatcgta cgccactccc atgaatagat caattgttcc cgaaataatt cgtttttaga
    16741 agcaagaatg atcagaagca gaatagacaa gaaaaccacc ggtgtgaaac taaaatcact
    16801 taataaaaca taaagtaaag ctagaacaat caagaagatt gtaggcaaat atgctcgttt
    16861 cacacgtgct gcgatccctc tgcccataat gattaaaaga aatcccaata gtatactagg
    16921 aaattggata ataaaatgaa atttcaatgg gttcaaacta tgaagccagc gaaactctgt
    16981 aaaagcttgc gggatagtgg cagaaagtac tagcatgatt ccagaaaagt ataataagac
    17041 aaccacgatt ttatgcgcaa tttctgttgc taattgttta gggataccag aataacgctg
    17101 atcaaaagtg gaaccgatat ttttgaagaa aaagacgatt ccaatcaaaa acggaatgat
    17161 gtagtagaac aggcgataga gcaagatcca cacaacgact atttctcttg gcacaccaat
    17221 agcggacaac ccgataatca tcattacatc gaaactccca atttctcctg gaatcataga
    17281 cacgattcct atcactgaag cagcaatgaa taatggaatt gtttgccaca gatcaatccg
    17341 gatttccatt aaataaccaa cagaaaggaa actaaacagg acaccggtcc attccaacaa
    17401 tgaaactagc aatagctgtc cttgagtttt aagtgaggta ccaccaagga atcctttctt
    17461 tttgaaagta gtaaataaaa agacgacagg aaaatataga ccgccgccga tcaaccatat
    17521 ccaatattgc ttcaaaaaag gagcaactgg cgtgaagact acaagtaaaa aagaaagcag
    17581 actgtaaata gaaagtccag ccatcaaaaa caagaaaact ttcgataatg cttggatcac
    17641 gtttctgctc tctttctttt gtccatatag ttcagagcga agccccacac taacaaaccc
    17701 gccgaaaccg gcaatattgt tgatcgtatt gatcagccag cttgtctcaa acaggtaacg
    17761 aggattctgc ttttgttcaa gcatccgatt caaaataata tcataaccaa ccataggtaa
    17821 cacagagata agaccaatca atagcattag tccgcttttc catacaggta tagtggcaaa
    17881 cgtttctcct aattgctgga tagatagagt tttaccgatg gacatcaatt caccgacaat
    17941 aatgacaatg acagaaatca aaaagattgt tttgaacagt cctaggtggt ttttcagcca
    18001 ttgtatggta ttttttaaca aataaatcac cttctattcc taatagttat gtttcagaaa
    18061 gaaattgaag aacttctcga ttgaagcgtt caggatcttt tctagctaat tcatgtcctt
    18121 gttctttcac aagcacaaag gaagcttttg gtatagtttt agctatatac aaagaatgtt
    18181 ttagcttgat tacatctttt tttcccacga tgatcaaagt gggggagttg atttttttta
    18241 aatcattttc tgtaagcccg atgtcatgca gcaaaagttt gataactaaa agatttttgc
    18301 gaagacttgg gcgaaataaa gagagtaacc atacccatgc ataatgaata ttactgatga
    18361 ctctggcaga aaaacgaacg cctttaacaa gcgtgtttcc agaatttaaa atcaatcgat
    18421 gaactttttt agggaagctg ctggcaaata ccagtgctag gtttgctcca tcactaaatc
    18481 caagaaaatc tgcctgatcg attttttcca gcaacatgat cgtattcaga tcctcggcca
    18541 ttaactgaaa attcaacata gatgcttcgt ttgtcgaacg accatgtcct cggctatcta
    18601 ctagatacac ggtatagtag cgttcgagta cagggacctg ttctgaaaaa aagcggccgc
    18661 tgccgtcatt gccatgcagt aaaaagagag ggaagccttg accgctcttt tcatagtaaa
    18721 tttcgctccc atctggcatt tttgcatatt tcttttcttt tttcacataa tcacctactg
    18781 tgtaaagtat aacgattata tcagtcattg ggcaattgtt tttactgaaa aacaacgatc
    18841 attttcgtaa gatgtcaaaa aatacaggaa aattcatttt ttcttaaggc gtacacagaa
    18901 atcaaggaag aaatagccaa gaagataaag aaatagtaag ataaaaaaag atgtcaaaat
    18961 aactaggtga tgaaatgaag cgaaaacgat tatctattat ccgtattctt ttttacatga
    19021 tcattctgac ggttattggt gcggtgttgt ttttaggagg aagaacttat cgttcggtaa
    19081 aacgagtgga aagctatcaa atagaaataa caacagctgc aaaaaattat catatggaaa
    19141 actacgagaa tttgatcaag gcgataatat tgaccgaatc aaaaggaaaa gggacagatc
    19201 tcatgcagag ctcggaaagt ctgcatggag aagctgagat cattgataat ccagctgaca
    19261 gtattgatca aggtgtcaaa tatttggcgg aaatgatagt agaagcaaag aaacaaggat
    19321 gcgatttatg gacagctgtc caagcatata attttgggaa aga
//
LOCUS       DACGLH010000052        18969 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.056, whole genome shotgun sequence.
ACCESSION   DACGLH010000052 DACGLH010000000
VERSION     DACGLH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.056"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(342..2312)
                     /locus_tag="IXT21_001942"
     CDS             complement(342..2312)
                     /locus_tag="IXT21_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869328.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2327..3193)
                     /locus_tag="IXT21_001943"
     CDS             complement(2327..3193)
                     /locus_tag="IXT21_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869329.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3183..3416)
                     /locus_tag="IXT21_001944"
     CDS             complement(3183..3416)
                     /locus_tag="IXT21_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4869330.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3428..5584)
                     /locus_tag="IXT21_001945"
     CDS             complement(3428..5584)
                     /locus_tag="IXT21_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869331.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5604..5825)
                     /locus_tag="IXT21_001946"
     CDS             complement(5604..5825)
                     /locus_tag="IXT21_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869332.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(5935..6273)
                     /locus_tag="IXT21_001947"
     CDS             complement(5935..6273)
                     /locus_tag="IXT21_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869333.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6673..10314)
                     /locus_tag="IXT21_001948"
     CDS             complement(6673..10314)
                     /locus_tag="IXT21_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869334.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPAPDN
                     QENNQETVINKSVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGELLKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10317..12266)
                     /locus_tag="IXT21_001949"
     CDS             complement(10317..12266)
                     /locus_tag="IXT21_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ4869335.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12308..13366)
                     /locus_tag="IXT21_001950"
     CDS             complement(12308..13366)
                     /locus_tag="IXT21_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ4869336.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13485..13940)
                     /locus_tag="IXT21_001951"
     CDS             complement(13485..13940)
                     /locus_tag="IXT21_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869337.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGMKKGR"
     gene            complement(14524..15327)
                     /locus_tag="IXT21_001952"
     CDS             complement(14524..15327)
                     /locus_tag="IXT21_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ4869338.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16307..17530
                     /locus_tag="IXT21_001953"
     CDS             16307..17530
                     /locus_tag="IXT21_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ4869339.1"
                     /translation="MESYQFQIFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17565..18332
                     /locus_tag="IXT21_001954"
     CDS             17565..18332
                     /locus_tag="IXT21_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ4869340.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            18524..18922
                     /locus_tag="IXT21_001955"
     CDS             18524..18922
                     /locus_tag="IXT21_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ4869341.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT         5733 a         3621 c         2747 g         6868 t
ORIGIN      
        1 aaactctcgc tttttcttca tcagtaaagt ttttatacaa ctgattacaa taatgaggtt
       61 tttgaagttt ctgaatacct atactaactt caaatccttc ttcatgttga attaggatta
      121 cttctgtgtc tctacctaga aatattcgat ctaaaatttt tatgtttggt ttcattattt
      181 aacctccact ataattattt cactcagtaa ggtttcactt tatgaaattt ctatagttct
      241 cttttcacaa aacgtaccat gttctctaag actgttttta gtctcaaata tacatgtttt
      301 ttcttatcct ttccttatac actcaaaatc aaagataaaa cttaaagaat aaactctcca
      361 ataacaaaat tattagtcag atccactggt ctcctgatct gaaatttatt acttcttcct
      421 gttgtcaaat actgtctcaa atcaacagaa agtttttgat catcttctcc ttttctagca
      481 ggagcaactt ttttttcata cttcattaac tctccactgg tcatctttcc aatgatagtg
      541 taattaaaat cactttcaaa actttggtaa atttttccat ctgtattgaa tatatcagct
      601 gtttcaatat cttctttgta ttcattgtct tctcctgaac cgattgtatc aaatagaaag
      661 gcaagacgaa ttccatcttc ttcaagcatt gttaatcttt ttttaattag cccaacggtt
      721 tcaatagata acttatcgaa gccgtgaatc ataacaatat catctttttt tgcttgtgat
      781 gtgatgaaat caaaggtatt taaaaattga gcttccataa cattggtttc tgctcttaga
      841 tcacctaagt tatagtaaaa ctgtaatttg tcacttttac cgatttcttt cagtgtcgtt
      901 ctttgattaa acatcctaga gaagctgtct aatgctgttc tcaatgtgtc ttttaataca
      961 ataatacgat cgacttcttt ttcagtacca gattttttgg cttttgtttc agcattagtc
     1021 aaatagctaa taaaatcccc aagaactggc attgtatcac tactgttcac caaaagtttt
     1081 gaacgttctt cataattaat atcccaaagt ttctgactta tataaaagtt acctaattct
     1141 tttttcaata aaactctagt tgcagaatct aatgttctgc ctgctaacaa ataaaaaatc
     1201 tgagttagtt tatcgagatt ccgtctatat aaatcaccaa tgtccttttg attaccaaaa
     1261 agctccaaag agttaagacc ttcttcagcc aaattaacct tttctattac actgtttaac
     1321 gaaggtttgg caacaaatgt tctaccagtt ggatcaccaa agtaatcaaa tttatttaag
     1381 acaatatgaa aagttcgttt atgatttgcc attgcattat tagcaatcaa ttgcccccaa
     1441 agtgatgatg ctgagatttt ttcatcatag cctttaattg tagcgttttt gtgtgatgcc
     1501 actaaaactt tacttgtaaa agaatcatcc agtgccataa aagctacacc acttgcgtag
     1561 ctagtagaca tagtgccgat agcccatcca ttcgcatcat ctaaaccttt gcgaattgca
     1621 tgatcatttc ctgcaaaatc cgaactcata gacgtatact catagacatt cccgattggt
     1681 tcacttaaaa gtctatcaaa cagatcacgt tgatctcctc catatgacat aagctttaat
     1741 ccatacattt tttcatcata caaccgttgg agtttaatta gctgttcgct gattttatta
     1801 ggagaatcgg atactaatag gatttctagc ttccaatcaa taattttatt tgccatgctg
     1861 tctagtaaag atgctgtatt caaatccata atccgcataa cagttttgct aatttcagca
     1921 tctgaatttt ttccttctaa attattttca taactattcg ttgatccccc aacattctcg
     1981 tgcattattt cttgttgatc tttttgtgac actggcttat ctgcttcaat tagataggct
     2041 tttacccctt ctactctcac ttcaggaata gctttaacca tccaaccaaa tccataattt
     2101 ctattcattc cataagtatt cactaatttc acaattgttc cccaccgctt acctgtccta
     2161 atgtaagatg gtgtaaattg ataaccaact aaagatgtta aatcactaac aaaggtatta
     2221 ttcactacct ttgaatttct agttttttgt tcttttttag tttttctctt ttttttgaaa
     2281 tttaatagtt gtgatctacc cataatttcc atttaatctc tcacctctat ttcagtgcat
     2341 aaatatcaga taatatttct tttgtttgtt tagctgacaa ctgagtgaca ctataataaa
     2401 gtcctctact cgtatagttt tctaaacgtt ccacaccttc atccaatacc atcttagatt
     2461 gagatttcaa gataagatat tggattactg ttgttttcac accatcaata tattctgata
     2521 caaatcgttc ttgctgatct attatgtcaa agactgcttt gttactcctt gcatttcgtt
     2581 tttgtatttt catatttcga gtttgtcttt ccgtattctg aggttgagat gaagtgattt
     2641 tgatttcagc taaggaacga tctctggtat tttgatattg tattgatttc gattcctgag
     2701 ccataacttc ttctggaaaa gcggttagtg atgtagcccc atctagattg aacattaatg
     2761 ctacactccc atctcgatat acgattgttc catcttgttt cacttttaaa atctctgtgc
     2821 ctcgaaatgg tttctttttc cccatagata ctcttgctga attaactact gatttgaaca
     2881 gtctaataga accagaaatc aatgaaccac tagatggatc aataaacata tactggctaa
     2941 ttagaataag aaatccaatt atcattaata acgttggaac aaaaataaat ttcagtttaa
     3001 tcgtatcaga aactaagtaa acaattgatc caagaacaac gactgcaaca actatagctg
     3061 atacaattaa tctttttgct gtaacagaat aggtgctatt gacatgcttt tcaagccatt
     3121 caactctgac aattgtttca gaaggttttt gatgtaagtt atgtttaaat ccatagtttg
     3181 agttatctgc catcagaaat acttcctttc acgttcaact tacggtaaac acctacagtt
     3241 atcactattg caacaataag caagccaatt aaagatattg acttgctcat tgtctcgttc
     3301 gtttgtggta gttcatttac ctgatacacg agctgtgctt cctgtaagtg ctgagaagag
     3361 gtcatgtttt cttgattgtt ttgaattact ggataaacta ttttttcttc tcgcataata
     3421 gccctcctta gttattatca gataagtgag cttgaatttt gtactgattt ccgtctggag
     3481 ttttcacaac tgctttttct ccatcaatag aaatcacttc agccttagtt gttttcccat
     3541 caatactgat gacaacatct ttcccaactt tgaactctgt agttggtgtt gcttcatagc
     3601 tactatttgt tgaagcagag ttcgtatttg tcccaatcga attttccact ctcgaaatag
     3661 atgtttgagt ttgagcctgt gcttgttgat tattatgact aataaagtag gacgatccca
     3721 agatcgttcc gcaagccatt gcagcgacag aaatgatagt agccatcttt ttacttgtaa
     3781 aatcacgttt tgaacttgat tgattttctg tagcaactgc taatgatttc gttttttctg
     3841 ccatttgagc tagatcactt cgggaacgag ataagtcttc tcttaaagct tcactttgtt
     3901 ctgccaactt ttcgctattt aatttataac cctctgctaa atctttgacc tctttcattc
     3961 gatctttata ttcttcgata gttttttgtt gctctacaag ttttgtttct aattctttcg
     4021 tgttgttagc tacctgcttt tcaacttctt ctttgttcgc aaaatatttg acaaccacat
     4081 ctgttgtttt ctcttttgta tcatggaact cagcttcatt ttctgacttt gttttttcga
     4141 caatctctaa agttttttct tgcacgtctg ttaatgattt ttcaatttct ggtttcaatg
     4201 aattgataat tcgttccacc acaacttctt ttgcttcttg gctttgtttc aatgcatcgt
     4261 ttttaataga ttcaattgct tcttggtact gttgatcaag actttcttca gttgcttttc
     4321 tatattcatc ttcttgttgt ttaacttctg gtaaatgctc agcatcatac cgttgctcta
     4381 gtttctttag ttcagcatcc aaataagcct gtttcgccat atcataatct ttacgaatga
     4441 tattggattt ttccatcact aaatcatcaa ttttagatgc ttctgcttcg tagctctctt
     4501 tagcaaccga tagtgtattt gtaaactgat tttgaccatt taatgaggtt gcttcctcta
     4561 tccgcaaaag ctcatcagct aacatttgct ttcctttgag gatcaattgc tccttcgtct
     4621 tatttaattc actgttgatt ttattattag caatagagat gcgagcgttc atactctctt
     4681 tagctaaaaa cagcaatggt ttcaactcag gtatggatag catctcctcg tttaaatcga
     4741 cagggtcatt aatctcatct aaaactacag gttgaaattt gtcagcaatg atctgttcaa
     4801 aatttttttc taaaaaatca gcatctaagt ttttcggtac atcactttca gatgtgattt
     4861 tttcagcttc ataaacataa ttttctttgt cactttggga ttcgggtttt tcatcaattt
     4921 ttggttgttg gtaggtttct gtttgtattt gtgtttcttc tatgcttact tcttggtttt
     4981 ctgcttcggt tggtttgtta tcatccacaa aaagagcttc tttttcatct ggtacatttt
     5041 ctgctacggc ttcaaattca acttcttcag aagagttgaa ggtctcagca attcgttcta
     5101 atccattagt agctacttct aattgctcta ctgcgctata gaagtctgta gcttcttcaa
     5161 cttctgattc aaacacatta ggatcatccg gaataactaa taattgacca ctactatcca
     5221 taaaacggac acctgctaat agcttctctt taaatttttc tggtagatca gctacataat
     5281 tcaaacttga cttcaataga acgacatact tctcagaatc gagaatagca tcatcctcat
     5341 ctataactcc ctcactgttg agcttttgta catcttcaat taccatataa tttttctttg
     5401 gtaaatcttt ggtcagcaac gcatcgaatt ctttccatac ctttgcttta gctttttttg
     5461 ccttcgcttc tttttcttct tttttgtaca cttcgagtaa ttgtggcttc aaagagagtg
     5521 caactacagg aactgtttct gccacataat taatagattc ggtaatatct ttgaattttg
     5581 ccattattgt aaaactcctt ttttcagtca atagttcttt tctgtttatc tagcatatat
     5641 acatatgctt tttcgaattt aataagcgtt tcaagtctgg ctttttcaat ctttttactc
     5701 ccatttcgat aatcatagaa ctggttaaca ttcatattca ttatctttgc cagttcggta
     5761 cctttcagac tgctcattaa tacttcgttg gcgtgagcta acattttttc tctatcaaaa
     5821 accatcttac atttactttc ttccctcact tctaaactac gcttggagcg tagttttttg
     5881 gtaaaaagta aattgaacaa atcggcatat tatccaaatt tgttcaattc acttttaacc
     5941 taacagttca ttttttggaa attttgaaac tagatagata ccaacaactg gaataagtgc
     6001 tgatatgata ttagcttttt ggttcaaatc attacctgtt ttagtaccca ttgtgtaaag
     6061 cataacggtt gtaattacag gaataataaa gattgcaata atcgccccaa catattttaa
     6121 tccatctccc cattttgcat caccaaaaaa tttcattgca gtgaatcctg gtttcaacga
     6181 aaaaacgaat gccaagaaat acaaaacaac ttgtactaaa gttttcatat caggcgtcat
     6241 tccgctaacc aaatttttaa tagaatcgaa catataaaaa cctccttttt aatattttta
     6301 ataagttaat tatatcatcg acaaatctta tttgttcact tgtcaatgat ctaactaaga
     6361 aaaagacgtc tcgatattta catctttatg ttatcataat aacataaatt ttcgatgatt
     6421 aaaagagatt ttccattaaa ttatttcact ttttttattg gctaaaaatg ctggtttatc
     6481 aagtttttgt ttatataata attctgttct ttaccagcat aagccctttt taattataca
     6541 actttgaaca gtaaatattt aattcattta tagacaattt aaaataattt ttttgcataa
     6601 atagaaattt tccttgacat cttcagttag aaatcaaagt aaaaagaagg agcaaatcaa
     6661 tgctccttct ttttactcgt cttcagactt attttcagtt gatttttgat taccgtattt
     6721 tacaatatag tccttgactg acatggtaga tttcttcact actttaccag ttttggatgc
     6781 tcctgtagca gtagccttgg caaccttagc cgttccttta cctattttaa tagcttcgtt
     6841 tttagcttca taggttatcc ctgctagact accttttgga gcttttgcta aagctttatc
     6901 tgctgaggtg taaacatttt taccagtttt tgatagatat tcaacggctg atttagctgt
     6961 tgatggtttc ttggttgatt gattagattt ccctgaatta tctgaagtag atttagaatt
     7021 atttgttcta ttatttttat ttgtcagttc aggttctcta attgattgat tgtctttcga
     7081 cattcctgat ccaatatttg tttcatattt atctgtttga ttaacattag gagtaggaat
     7141 gaactttttc ttcacatctg attgttcttt atccgtttct tttgttagcg attctctgtc
     7201 tttaagtgct gtgtcattcc gaatagcact attatcaaag ttgctgttaa gctctcttga
     7261 ctgactttca ccagatgctc catctcctaa ttcccgatgc actctgtctg acacgtcctc
     7321 accactcatt tcattcctca gttcttgatg agtttgatct aaagtaatct cgtcatcttc
     7381 attctcagga attgcaacag caattggcgt atgtcgttgt tgttcgagtt ctctatttcg
     7441 atctgccaca agcgcatctc tcgtctcaat gatattccca tcctcatcga ctaaatctcc
     7501 attcacatta tatccatcgc tgtgtttacc aagtttatta ccaaactcat caacaagatt
     7561 tccatcttca tcaactaaac gccctttttc gtcaactaaa cgtccgttac tatcctgacc
     7621 aaaatcacca tttagcaacc tgcgtccatt ttcgtcaaca agatttccat cttcatcgac
     7681 taagcgacct ttttcatcaa ttaaacgtcc ttcgctatcc ataccaatat taccatcttc
     7741 actatcttcc aatggtttac cattagtatc tataaggttt ccatcttcat cgaccaaacg
     7801 ccctttttcg tcaataaagt tgcctttagc atctttaccg tatttactat cttttaataa
     7861 ttcaccattt tcatcgacaa agtttccgtt ttcatcaacc atacgtccta aatgatcaat
     7921 atattgttgg gagttattac gtagttgatc ttgacgaact aaatctttga aagaggtatt
     7981 attgattgtt gaaccaactc cgccaccgtt gaatgacatt ccttctggtg tttcaggaat
     8041 tactttatca gcaatggact tagcatcagc attgctatct cccatattgt tcaaacgttc
     8101 agccattttt tgcgctccaa ctgctccaag acctcctgca accatatctt ttactgagct
     8161 gttgccacca tcataatagg gatttttagc tccttggctt ttcttgattg ctttagaaac
     8221 gccgtccaca actcgcacag catcatttct attaccaaat aataacttag cacgttcatc
     8281 aaacccaaac gctacttcaa gtggtaagcc aataagcata ttaatcaaat tatttacttt
     8341 ttcgcctttt ttaaagacta aaagatatcc tacacctaaa atcatagcag atcgaacaat
     8401 atttgctacg ccatcgccaa ctaatgtatt aggaatgaat cccacagcag tatcaccaaa
     8461 tgcacgaata aaagtaatca gaaagatagg taattgtagt ccgatcagag atgttgtaac
     8521 aacacctaat gaaattgcca tcacacctgc tcctgatgca agtgatcctg ttagaactac
     8581 ttcccaaatc cgaataaatt cacgtttaaa tgctttcacg atattcacat tgaacatcgc
     8641 ccaagcacat gctaagtaaa gaactgctaa aacgattgat tcagcgttca tcgctagcca
     8701 cgagccaaac ttacccatcg ctccatgacc aactaaggat gctgtacgcc attctgtttt
     8761 tactgttgac atattcttag cactgccttg aattcctgaa ggagcgatat tatacgcata
     8821 aaacttagct cctgatgtat caagtgccga ttggagcaat aaagctaccg actgtgtgct
     8881 aaaagttgca ttgttagcaa cgccccaagg ttgagtagca tcaaagttat gcgctgttcc
     8941 ttttccatca ggcttgcgat tgtcgtctgt cacattttga tttgctgacc aaatatatcc
     9001 atttaaattg gttggtttct taattgcact tccttctttt ccatccccta aattatagcc
     9061 tgtccaaaca gcaggcgctc ttcccatata agcatcattt tcggcgacat taacatcacc
     9121 ggcataagta ttaatattga aagtatcatt cgatgaccat tcttttaata gagccatacc
     9181 gactgaatta gaatctagat cattattttg tctggcataa ctgctagtat tgatcgctct
     9241 tatcattgca gattttttag atggttcata atctgtatca acgaacttga tttcggatgt
     9301 aggctttccg tcaggtacat caccaccagt atcagatgat ggagcaagat tttgagtaga
     9361 agcccaaagt cttgtgttaa tgatgtagtc agaaacaaca tttgttggta tatctccatc
     9421 tttttttgct gactggatgc cgttgatata aactcccaat agcgatcctg ccactggtaa
     9481 gaatataaac aatacaaaaa tacgaacaat ccatttttca agtactggtg ataatccctt
     9541 cgagttaaca ccatctttcg acaataattt cattccttga taacctagta ttgctgtgaa
     9601 aaagagcata gataagcttg ctatcgcttt aaataattca ttatttaatc ccaaattgtt
     9661 aagcaagcta gttaatgctt tagataccgg attagataca tccgatccac cttctcccca
     9721 ccccataaga gaaaatatat tcatactcat gaaaaatttt ccaaggagtt catttatgga
     9781 ttcagcaata ttagaaagga ttaaactaat tgatccgata aaactaccaa tcgtcctacc
     9841 aatacttcct agaatggatg atgcttgact ggcaatagca tttcctgaga ctgtttcaat
     9901 atatccatag taactataag ttgctagagt agatgctagt tgtgtacctg cattcccccc
     9961 tgcaatcgaa ttaccttgtt tagaaatatc agaataagga aaatcagcac tgataccacc
    10021 ttgtcctaac aaattcattt gtccagtttt agcagcacta tcattgactt ctttatctcc
    10081 catcaagata cgagcatata gtgtccacat cccttgttct gccggtttac tttccgcata
    10141 agcaacgctc ttatttataa ctgtttcttg attgttttct tgattgtcag gagctggatc
    10201 attcattcca aatggagcgc ctaaagttac tgctaatacc cctccaataa ctagacctct
    10261 tacgacccat cgttttattc taaggttttg tagtaaatat ttcttgaatt tcatagctac
    10321 gcccctccaa tcgaaaatac aatcttgttt tctttacttt cagcaacgga aggctttgca
    10381 ataatttcag cattagaatc accttttcct tcaaacgctc cagcttcact caacttaaca
    10441 tcgaatattt ttgcgtttgg ataagtaatt attgcttcag taatcgcttc ttcaaactca
    10501 gattcaacta aatgcaagat attccttgca ccccctgatt tagaatcctc ttgtgaagcc
    10561 atgtcaaaaa ctaagtgcat ggagagatcg tctgcataaa aattttgacc atatactctt
    10621 tctttcctag gtggaagacg taagtctata tttaaatgtt ttcgtactaa ttcagcactc
    10681 ttcaacattt cattataagc aatttccaga agagtttgtt cggataaagc ccaaaatgga
    10741 actattcgtc caaatcggtt aataacttca ggctttagac cttcaccaat caaggcttgt
    10801 ttgatccctg atgcgacttt tcggtattct ctttttagtt cagtgctgta ttcattagaa
    10861 ttttccttgt ttgtaacacg agatagtgag ctatatcggt ttaacgaatc gaacatctcc
    10921 gcacctgcat ttgttgtcgc aaataaaatt gtatttgaca atgaaacggt gtcgtgaact
    10981 tctcgatcat cccatcctct ttcagtaaaa cttatcctac cttcatctaa aattgataaa
    11041 aaggcatcga gtgtttctac accagctttc tcatattcat cacacacaat aatcgcagaa
    11101 ggatcatgcc ttacatgagt acctatgagt cgtctaaaac gcattgcatc atctttggtt
    11161 ttgtaatccg gcatattaac taagagaact ttattttcat cgccataaag tcctcttgcc
    11221 acggctttag ccattgcagt tttaccgacc cctgttgatc ctgtaaacag agcagttgct
    11281 tttggttgct ttcgaccttt aaaacgatca aatccaagct tcatacgttt aaacaacatt
    11341 gttatagtat aaactgctaa cggttgtcct tttatggact ttaattcctc ttgaagtttt
    11401 tccatatcaa aatcagaatc tagctgaata ttatacttac caaatatttg caatagactt
    11461 tctcgattat aagcatttcc gtctactcga tcttttgcaa tcaaatcctc cattacagca
    11521 attgatttag ccggttcgga caaatcctca cgataaagtt tgtttgcttg tacgatttct
    11581 tgaagtaatt catcaggaat cctttgcgat aatctaaaag aagcctgagg atcatcttta
    11641 acagaggatg tatttagcca ttgtctcaaa atatcgacga caacatcgct ttttagttca
    11701 ttcaattcta acggttgtag ccgtctccct aaggcatcat ctcttttgat atagttttca
    11761 tattcttttc ttgtagtagc ggtaatcatt tgaagtgatt catttgctaa cggttctttt
    11821 agcgaatcac ccccaatttt attgccactc ccaaaagagc taacaacttt atgaaactcg
    11881 tcaatgaaga ggacaaattc attatctttg tcctcagctt ttaaaattcg ctcatattct
    11941 ttcaatcttg gaattaatgt ttctaaacgt gtttggagga catcatctcc cccaacacgc
    12001 aattgtccga ttgataattt atatacttgt actttctttc catcttgtcg ctgtctttca
    12061 gcccattcac ttgcaagtgc tgttttacct gttccggctt cacctaacaa gatagcttgc
    12121 ggtgtttccg gtctattcaa gatcagtgac aactcatgga gttcgtcatc acgtcccact
    12181 aacttatgtt ttggtggttt cattggtgtg agagcatgat taaattcggt ttgaatattc
    12241 ttaaaaaact cggtactatt ttccatctgt ttgtgttcac ttcctctaaa ataagaataa
    12301 tttacttcta gtcagtcaaa gttaggtact taaatgtcgt cttcatgtct ccaaaagaat
    12361 ctttgttaaa gtcagcattt ttgtattgat caatcaattt ttgtgtatca ttgattgctt
    12421 taatttcggt ctccattcga ctgtctagtt gacgaatact ttcttgcgaa tctttatcgt
    12481 caggattttg ttgtaaacgt tccagatact cagcttttgt ggcttcaatt ttcttgatgt
    12541 tagtatttga agcatttaac tgatcttcta gcttatttac aacattattc ttacttgtaa
    12601 ctgctacaac ttttgaataa tcaatatcgc catctttggt aagaaagctt ccttgataga
    12661 cattagttga atctgaatat ggatttattc tgaaattgat attatcagga gtattaacag
    12721 gttttgcttt tttcccaaaa gaaattactc cttcattatt aacatcattc atagaatatt
    12781 gagaaagtgc ttttgtaact gaatctgctt cattcacatt gactttggta tcatcgtcag
    12841 ctgtatcatc accagaaatt agtgatactt ggtttgcaat aaaaacttga taagctcttt
    12901 cctctaattt accttttagg aataagaaag catccccatc agtacttaac atcccatagc
    12961 tcatttcaag cttcttagga cgatcttttt cttttgttgc catatataag ttatagtttt
    13021 ttccttgcgt agataacaac tttctagcgt tttgggagta accaagtttt actactgtta
    13081 catctctatt ttgatctgtc cacacatctg aaatagtgat ttctgctcct gatcgagcaa
    13141 actccaatga agaccctata ggtgttgtat tgttcacgag ataataatta tagtttccta
    13201 attgataaat gtagctaccc aagcctgtta ataaaaaaag cactcctaga aaagttgcaa
    13261 gcataatgcg taatccgtga cgtgttctct tatccttatc accaagcaac cctccaataa
    13321 tactgtattg atctttggta gatgttgaat tgacgttttt ttccatcagt ttgtctgccc
    13381 cctttcataa aagagcaaac aatcagacgt tttttcggta attctaggtt cgggattttg
    13441 aatcatttcg gtatattctt ccccattaat atttaacaca aaaattatct tcctttcttc
    13501 attcctttct tttgctcttt attttccaaa taatcaatga tttttgctat tgtaccaaga
    13561 tagtaattcc cctcagcttt gttttttcgg attttgctaa ccattggagt aggtacaatc
    13621 tcttcatctc tcaattcact gaaagatttt ttacctagat attttccaat aacagatttt
    13681 aaaaaacgtt caaaatcttt ctcgtaagtt gttttcttgc cttttagttc atggttcata
    13741 ttgtcaacga cttttgcggt ttcaataatt gttttagttc tcagcttaat aatttcatct
    13801 ggaattttca atcctcgtaa tttcatcgtt gtactaactg gtaacccatg ctttactgca
    13861 tcagcaatac taaaactttt tagatcatct agtaagctct ccatatagtt aacaaattca
    13921 tgccaattaa tagcattcat tgtctcaccc cttcattttt ttaaaatgag gttcgggcgt
    13981 aacacattta tgttattata atatcataat aacataaata aatcaatcag caaatttaga
    14041 tatcccttaa agtatcagca attattcaac aagatattta ttttagctat ttcagtcatc
    14101 ataaaagact aattagcaat aaaataacta atattatcca tttatcagac gaaaagaaaa
    14161 aacttatcta attactctat attaactttt aacaaatagt tgacttgtcc aaaaatgtta
    14221 ccttcaatct cacaaagata ttaagatgtt tcctaccaaa actgatatca ccggactttt
    14281 tattctgtgt tgtccaaaag tgttaccttt agtcgatcaa caaattaaaa aaaattgttt
    14341 ttttcttgac ttgtccaaaa gtgttaccca tagttgttaa aatttgtata gattagccat
    14401 tctttcgaat attatttaga ctgaaacttg cttacaaaaa aacttcttgc atttattgtc
    14461 caaaagtgtt acccatactt ttttaaagaa gcaggttgta ttaaaattac aaccttcttc
    14521 ttctcaatct tcttctaacc aattatgcat tgaaatggtt ggcaagtttg attctggctc
    14581 actaaaagtg aatctgagcc gatcaactct tcttcctttc tttgcataga ttttttcata
    14641 atcaaaagtt tcaaatacct gtattccgtt ttcatcagga gctaaaaatt cttctttcgc
    14701 ctgctctagt attcttttgt caatatttgt cattctataa cttgaaggta tacttaatgc
    14761 tcttctaaaa tcttcaactt tgactaccca ataaccattt cgccactctt tatttctgta
    14821 ttgcatgaga tgacgataca attcttttgt gtagctactt cttagttttg taaaattatc
    14881 aagttcaaaa cgagtaaagt ttttgccaat tgagtttaag ataaattcaa attcctcatt
    14941 tgccttaatt gtaactgttt ccttctgttt atctatccgg aatttttgaa aaagccacag
    15001 ctgttcatag acgtctgaat cttcaaaacg gtaggttaaa gtacctagtt tgtcactcaa
    15061 agcttttaac acagaataaa aactgtcctt ttctcttctg tcgtatttga ccaaataacg
    15121 aaagaggttg aaatcaaaag ttacttctgc tgttccttga cgtttcattt ttgataaaat
    15181 agcccaaaac acatccattt caacaggctt aaatcctcgc aaagctgttg tattcagacc
    15241 attgttataa cgcacaattg catttgtttg ttctgatact aaatctacat cactatcaat
    15301 aatcttttcg gtaacttctt gagacattga ttactctcca atcttctttt tgcgctcctt
    15361 taatcatgtt aatctggtac taattaaagg aaacaatttt ttctgcttgt tacctaaatt
    15421 aaatcacaac taaaggtaac agtcaacaaa gatttgttac taatctaatt ttctattttt
    15481 tcgccttcca aatcaagaaa accgaaggta acgtcttatc ctagtgtgat acctcaaatg
    15541 tccacttttg ttacctttgc tgtccaaaag tgttaccctt actgtccact tttgttacct
    15601 ttactgtcca aaagtgttac ctttataccc ctttaatact tactctccca agagattaag
    15661 cctcccctaa cagatatatt aacagatatt taacagatat attaacagat atttaataaa
    15721 ggaggattac tcctctctta tttcaaaatc ttaaaaataa aaaatcttaa aaaaaactaa
    15781 cactatctag gagtttattt tgcttgtgta actttgaata attgaaaaaa aggaaaagga
    15841 ggaagcttca gcttcccctt ttatttatat ttataatata tattttaatc tttaaaactt
    15901 tagcaacctt caaaaaggct ttatatcaat cttaaataga ttttagggtg gacataaagc
    15961 gatgcgaaca tggacaaaaa gcgatgtgaa tgtggacaaa aagcgatgca aacgtggaca
    16021 aaaagcgatg tggtattccg aatatatcca aaaaaagaaa aagtatgtga atttatttct
    16081 aaaaactaga aaaatatttg ttttttaatt ttggtaattt ttataatttc gtattttttt
    16141 gaaaaggatt tttatattga aaatgttcta aaatagcgat ttctgctgtc ttatcaatga
    16201 aaattgatta aaaaaataaa atgtgtttta cagagattga gttatgttat aaatttacga
    16261 tacatttctt attgtatcgt atcgaaaaca ggtctatact tattcaatgg aatcgtacca
    16321 gttccaaatt tttaagctcg taccaagctt agaaagaaga ggcaatcgta tgaaaataac
    16381 agaaaagaat gtaaacccga aatttgaacc taccgaaagc aatctaaaaa agtttgattt
    16441 aactgttact ttatcgaatc aacttgtaga tagcattgtt gaaatggatg ctatgccaca
    16501 aaaaatttat ctttatgcta taggacatct tgaccctaaa aatccgcctt ttaataatac
    16561 tgttaaagta tctaaaaagg ctttatttgc gttgatgtct tctagttcat cttcttcaaa
    16621 acattcaaga ttaaaacaag ttattgaaaa attgcatcag caatctgtca ttaaaattca
    16681 aagaactaaa cctaacggta agactgaata caaagtcatc tcaccgctag aaattactga
    16741 attcaatgat ttttctgatg cagtatctat taaatttagt agttcaatta tgcctttatt
    16801 aacgaactta gaagatcatt ttacgcaata tacatttgat caagtagtta acttgacaag
    16861 caagtatgcg atcagttttt ttcaattatt cactaaagat tttaatctgt atgaagcaaa
    16921 tagagataac ttaacatgga gtacaaaatc acttgataga ttaaaaaatc ctgttttctc
    16981 cattgcagat ttacgaaaga aatttagtat tgagaaaaaa tataaagctt ttcgagactt
    17041 tgaaagacgt gtaattaaag aaccgattgc ccaactgaat aaatacactg attttaaaat
    17101 tgactatgag cttatcaaga agggtcatac ttatacaaac ataaagttct tgcttacacg
    17161 taccatgtca gttccggtac cttataagca agaacaaaga gaccctgctt atttaaaagg
    17221 cgcagaagat aaaacagaga aaatgaatca atcgtacttg aaagctatga atagcaaata
    17281 cactaaatta ctaatcaaaa actttttgat cagttctgcc gacttatgcg atcttaaact
    17341 tatgagccga cttcagaata aagtttatcc gctttatgat cgtttatcca aagatagagg
    17401 atttgaatca gttgtcaatc acctatctta cgttgctact cataaggagg attacacaat
    17461 aaaaaatact gttaaatatc tcgaaaaatc aattacgagt tatttgcctt atgtgaacct
    17521 ttatgattaa caactgaaaa aatgcgtgaa ggaaggaaaa acttttgaaa aaagatgaac
    17581 tgacaaagta caatgaggag ttcttagcaa caattgacat gaaaatggat ttaaagtcgt
    17641 atgaacttta tcaattgctg ttcagttata tctcacccac aaagctaaag aaaacaggcg
    17701 tagtagattt acctgttaaa gcgattatta attacatgga tgttgatcaa aatcttgaaa
    17761 cggaactaaa aaccatagtt aacactttgc aaagacaagc agtttatgaa atttcgaatc
    17821 tagacaaaaa atataagacg tatactactg tatctgctat tgatcggatt cattgggatt
    17881 tagaagagca tagtgtatct ttcagttttt cagaagatgt aattccctta attgtctcaa
    17941 tcaaaaagaa gtatccgttc tttgtttttg aagacttagc atcttttcaa aaaaaatata
    18001 gcatcgtgtt atacaaatat ttggcgatgt attataaaaa atatcttatc gccaaaaata
    18061 aaggagattt tgagaaagct gaaatatata agaatcccct gattgaaata gttaaaattc
    18121 gtgagattac gaaaacagaa aatgagtata aaacaatatc gaattttgat agacgtgttt
    18181 taagggaatc agttgaggaa attaatgaaa aatcaatgct gaatattgat tttgctaaag
    18241 ttagaaaagg cagaaattac aattttgctg tccagtttaa tatttctagt gagtttgatg
    18301 aaaaaactta tagagaaaaa aaattaggtt aatgcaaaat atatcttact cgtttgaaga
    18361 tgcttcagac gtatgctaag atatatagtg ccttgggagc aaggtattaa tctcctttag
    18421 aggttaatta gcttgtgatc gcctatcaat ttttttggta ggcgattctt ttttttgaaa
    18481 caaaaaagca aaaattattt tttaattaga gaggtgcaat gcgatgaaat actttttttg
    18541 tgcagtattc gaatggaaag gaaatgaata tgcagttaaa ttcccagatt ttccagactt
    18601 atatacacat ggtgaaactt tagaagatgc ttttgagatg gcgcaagaag tattggaagg
    18661 atacttggtg cttttagagt tgcaagaaaa agaaattcca tcgcctacgc ctgttcaaga
    18721 gttaaatgca agaatcaagc gatctgagaa tgttaattta tttgtcgtaa aagctaatac
    18781 gaaagttgct aaagctcgaa acaagcaaaa ttatgatcga aaatcagtga ctgtggaaca
    18841 ctatttaaat gttttagcgg ctgaagagaa tattaatctg tcgaaagctt tgaatgattt
    18901 gttgagagaa aagttgattt gatgtctctc agatgatcat attaagcaaa aacagcttgt
    18961 tttaggacg
//
LOCUS       DACGLH010000053        18965 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.057, whole genome shotgun sequence.
ACCESSION   DACGLH010000053 DACGLH010000000
VERSION     DACGLH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.057"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(20..250)
                     /locus_tag="IXT21_001956"
     CDS             complement(20..250)
                     /locus_tag="IXT21_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869342.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(686..1252)
                     /locus_tag="IXT21_001957"
     CDS             complement(686..1252)
                     /locus_tag="IXT21_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4869343.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1252..2208)
                     /locus_tag="IXT21_001958"
     CDS             complement(1252..2208)
                     /locus_tag="IXT21_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ4869344.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2208..2864)
                     /locus_tag="IXT21_001959"
     CDS             complement(2208..2864)
                     /locus_tag="IXT21_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ4869345.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3234..5252
                     /locus_tag="IXT21_001960"
     CDS             3234..5252
                     /locus_tag="IXT21_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4869346.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5679..8093
                     /locus_tag="IXT21_001961"
     CDS             5679..8093
                     /locus_tag="IXT21_001961"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ4869347.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8441..9994
                     /locus_tag="IXT21_001962"
     CDS             8441..9994
                     /locus_tag="IXT21_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ4869348.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10375..12159)
                     /locus_tag="IXT21_001963"
     CDS             complement(10375..12159)
                     /locus_tag="IXT21_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869349.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12174..13895)
                     /locus_tag="IXT21_001964"
     CDS             complement(12174..13895)
                     /locus_tag="IXT21_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869350.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13995..14756
                     /locus_tag="IXT21_001965"
     CDS             13995..14756
                     /locus_tag="IXT21_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4869351.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15723..15991
                     /locus_tag="IXT21_001966"
                     /pseudo
     CDS             15723..15991
                     /locus_tag="IXT21_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16663..>18965
                     /locus_tag="IXT21_001967"
     CDS             16663..>18965
                     /locus_tag="IXT21_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869352.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         6231 a         3364 c         3805 g         5565 t
ORIGIN      
        1 tcattaaaaa gcataaccat tactccgatc tgtttttaaa attagtttct acctctaata
       61 tatatcgagt ttttattttg ccttcagaga atactgtttc tttttttgca gttacaggtt
      121 gtttattttc ggaaaaagct aatatagcta agattgaaac atccatctgg aatttatctt
      181 ttttactgct ttgatcataa aagtctgcat attcatcact gatatttttt ctaataaatt
      241 cttccatcat aaaaatcacc tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg
      301 atatttgagg ggcaaaaaag gggcataatt tgactgatat taagttagct attgatatat
      361 gaatgacata caaaaatata aaatctttta aaaatgctta tatatcaatg tttttgccat
      421 tcttttatat atctgttata tgtcaaaaaa atgtgttcaa tttcaatttg tatttgaata
      481 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaatc aaaaacagct
      541 tctcatgttt ttgatttggt tggacttatt accgcagata taagcttttg agcaccgtga
      601 ataaagaggt tatgaaatca acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat
      661 atcatttatg ttttgcgttt tttatttatc tttttttctc aatacaatat aaaataggcg
      721 gctggttctt ttggttgata aaccgataat tcagcacatt atatgtttcc tgtggcagtt
      781 tggaacaaaa agaatcaacc atatccagtt ctttttctcc accttcatga ccatagtaga
      841 cgacaaggat caatcgtccg cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc
      901 gtgtagtctc tggcatagtg atgattgctt tatcactttt tgggagatac cctagattga
      961 aaatccctgc tttgagattt tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt
     1021 gtagaaacaa tgagacacgt tcttctaact gacgctgttc tagcctttca cgagtagcat
     1081 cgattgcttg ttgctggata tcaaaactat agacatgacc agtttttccg atgtgttctg
     1141 ctagaaaaac cgtatcgtgg ccattgccca tcgttgcatc tacgacgtga tcccctggtt
     1201 gcaagatttc ttgcagtaat gtatggctga aatgcaaagc agtttgcaac atcagacgct
     1261 cacctttttc cgtacatcat attttccttg gaagctatct ctgcgaagaa gttcttcatc
     1321 aattgcattc aagacttccc attttttcaa gctccacatt ggtccaatca aggaatccca
     1381 cggcgcatcc cctgtcaaac gatggatgat gatctctttt ggtatcattt ccagctggtc
     1441 gcaaatgaca ttgacatagt caggacggct cattaattga aggcgacctt catgatagtc
     1501 ccgcaacatt ctcgttttgc gcatcagatg catcaaatgg agcttgattc cttggatatc
     1561 tgaatctaaa atggtgcgtc tgacattttc tttcatcatt tctaaacttt cgcctggtaa
     1621 tccgttgatc aaatgcgtac aaacacggat attatgtttg cgtaatttag ccacgccatc
     1681 taaataagtt tgataatcat gtgctcggtt gatcagtttt gaagtttctt caaaagttgt
     1741 ttgcaatcca agttctaccc ataaatacat ccgctcgttc agttcagcaa ggtaatcaac
     1801 tacttcatct ggcagacaat ccggtcgtgt accgactgat aatcctacga cacctggtaa
     1861 attgactact tgctcaaatc gttctttgat tatttcaact ggcgcatggg tgtttgtaaa
     1921 attttggaaa taaacgatgt attgttggac atgaggccat ttttgatgca tcatgtcgat
     1981 ttctttctgg aattggattg gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc
     2041 agatacactg caaaaagtac aaccgccatg ggcaacagtc ccatctcgat tcggacagtc
     2101 aaatcctcca tcgatcggca ccttgaaaat tttcccgcca aactcatttc gtaaagcgta
     2161 attccaagaa tgatatcttt tatttccttc tgaataagga aaagtcatta aaagtccttc
     2221 tttctgtaag agttatctat ttgttttctt ttttctctat acactggata agtcaggcac
     2281 agccaagaaa gactgagtaa gtagccgccg atgatatcag taggaaaatg aacgcccaga
     2341 taaatgcggc ttatcccgac acttactata ataaacatca tcagcaattg aagcaatgtg
     2401 cgtaaggttc ttttttgaac aaaagctgga agcagaaata gtaaagaacc gtaaagaatc
     2461 atactgcctg ctgcatgtcc actagggaaa ctgtaacttg tttcaactac taagtgcgcc
     2521 aatgaaggac gctctcgcat gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt
     2581 acaataaatc cagaaacgaa ccaaaatgtt tctgcccttt ttttcttata ccacatccat
     2641 aaagccgcac aagcaaataa aatgatgacg gtaagaggat taccgaattt agtcataaag
     2701 agttggtagc tgttcagatc aggataagaa gctctgacaa tgcctgtaaa ccactgatcg
     2761 aaaccagaaa gccacgacag atggaacttt acgatccagc ccaaaatgaa aaacaggaca
     2821 agaaagaagc taccagccac ctgaaatgat gttttatatt tcatacaatt cctcctttga
     2881 agcaatgact tattatagca tttttggtcc agcgaaaaaa acggtaaaga gataaaacca
     2941 atgtatgatt cctctttgaa taaccaatat tcttataaaa aaaataactg taatcggaaa
     3001 gacatttttt ggtaatgcaa atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc
     3061 cttttaaaca agaacgtaga cgtaggaaga acttgaaaat gaacagaagt tgttcattac
     3121 ggtaaattac aatatgatta caaaaaacaa caaagctctt aaataacttt taataaatat
     3181 taagagaagt tataattatt aataatagat aaattgaagt tggaggatag aagatggaaa
     3241 acattgcaag aaaagaacgg agacgtttag aacagaccaa acgttttcga aaagcaaaac
     3301 gaggggcagc aatcgtggga actgcgatgg taggctgttc tgtcgctgcc ccattagtac
     3361 agccggttcc tgttcaagcg gatgaaacac ctgttcagtt cggcgcaaga atcaatacag
     3421 cggcatttat cacagaaatt gctacttatg ctcaaccaat cgctcaagcc aacgatctat
     3481 atgcatcagt gatgatcgca caagcagttg tagaaagcgg ttggggcagt agtgcgttat
     3541 ctcaagcacc ttattataat ctttttggga taaaaggcag ctatcaagga caaaccgtct
     3601 acatggatac gcttgaattt ttaaataatc aatgggtaaa taaaaaggag cctttccgac
     3661 aatatccttc ttttgctgaa tctttcaatg acaatgccta tgtacttaga aatacttcat
     3721 ttggcaacgg ttattactat gccggtactt ggaagagcaa cacgaaatcc tatacagatg
     3781 cgacagcttg tttgacaggg cgttatgcga ctgatcctgg ttacgcaggc aaattaaata
     3841 atatcatcgc aacttacgga ttgacccaat atgatacacc agctagcgga aacgcaggcg
     3901 gtggagcgac tacaggtggc aataatactg gaagtgcagg tagtactggc ggttcaagca
     3961 attctggtaa tacaaactca ggttcagcaa cttcgaccac ttatactgtc caatctggtg
     4021 attctgtttg gggaatctca aataaattcg gtatcacaat ggatcaattg atccaatgga
     4081 acaacatcca aaacaatttc atttatccag gacaaaaact gacaatcaaa ggtagccagg
     4141 aaaatggctc ttcgacgaat aattccggta acaataccaa ctcttcagga aatgctggta
     4201 catcaaacgg aggacaaaca actggtgcga aatacactgt tcaatctggt gattctgttt
     4261 ggaagatctc aaatgatcac ggaattacga tggctcaatt gattgaatgg aacaatatca
     4321 agaacaattt cgtttatcca ggacaacagc taatcgtatc aaatggcggt tcttctaatg
     4381 ctggagcaaa tactggctca aatactggga atacaagtgg ctctagttcg tccagctcaa
     4441 gttcttctaa tacagcttca ggaacgaaat atacagttaa agccggagac tctgtttgga
     4501 gcgtctcaaa taaatacgga attacgatgg ctcagctaat ccaatggaac aatatccaaa
     4561 ataactttat ctacccagga caacaactga ctgtttcaaa cagtggatca acaaacagca
     4621 cgtcaggtaa tacaggttcg tcaaccaata caaataacag cagttcagtt aatactggca
     4681 gcagttcgtc tcaaggcact tataccgtga aagcaggtga atctgtttgg agcgtggcaa
     4741 ataaaaacgg catcacgatg gatcagttaa tcgaatggaa taatatcaaa aacaacttca
     4801 tttatccagg acagcaattg atcgttaaag gtggaacttc ggcaaataca aatacaggtt
     4861 caactacttc tgcagcgaaa ccaaatacac cgaatacgtc tgctacgaca agtacatcat
     4921 ccactggtaa tacgatgtat accgtgaaag cgggcgagtc agtttggagc gtagcgaaca
     4981 aacatcatat cacgatggat cagttaatcg aatggaataa tatcaaaaat aatttcatct
     5041 atccaggaca gcaagtgatt gtgaagaaag gttcatctca atcaacaaac caacaatcag
     5101 ttactggttc gaaaacctat actgtgaaat ctggagaatc tgtttggagt gttgctgaca
     5161 gtcatggaat tacaatggct caactaatcg aatggaacaa tattaagaac aactttattt
     5221 atccaggtca aacgttgatt gtagctaaat aaaatagctg ttcgaagaaa taacgattga
     5281 attccacaaa aatatttgtt actataaggc tacttaaata tgacaacact tgaaaaggaa
     5341 gtagtaaaaa gactgaagtt tcagagagcg catggctggt gagaatgtgc acggatgctt
     5401 ttgaactcgc ctttgagttg cccttgctga atacccgagt aagcaagcgg cggtgacggc
     5461 cgttacaacg ttcgaggcct tttcactaag gtaaaattag gtggtaccgc gtagattacg
     5521 tcctataaaa agagcaatct ttttatagga tttttttatt tcttttgtga agtttgagaa
     5581 aacaaagcaa aaggaaataa aaataaacag attctgccgg ttttttcgtt atatttataa
     5641 tagaaacaag tgttttcaat agaacaggag gacagcaaat gagttacaat cataaagaaa
     5701 ttgagaaaaa atggcaaaaa tactgggcaa aaacgaatac tttcaacacc catgatgatc
     5761 ctgaaaagcc aaaattttac gcactggata tgtttcccta tccatccggt caagggctac
     5821 atgtagggca tcctgaaggt tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc
     5881 aaggatacaa cgtgctgcat ccaatggggt gggatgcatt cggtctgcct gctgaacaat
     5941 atgcgctaga tacaggaaac gatccagctg agtttaccca aaaaaatatc gaaacattcc
     6001 gacgccaaat caattcttta ggatttagtt atgactggaa tcgcgaagtg aatacaactg
     6061 atccggagta ttataaatgg acacaatgga tctttacgaa gctttatgaa aaaggtctag
     6121 cttatgaagc agaagtagcg gtaaactggg tacctgaatt aggaacagtt atttcaaatg
     6181 aagaagtgat cgatgggaaa agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc
     6241 gccaatggat gctgaaaatc actgcttatg ctgatcgctt attggatgat ctagaattag
     6301 ttgattggcc agaaagcatt aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag
     6361 caaatattga atttaaagtg gctggtacgg ataaatccta taccgtattt acgacacgac
     6421 cagatactct attcggtgca acttactcgg ttcttgcacc tgaactagat cttgttagag
     6481 aaattacgac ccctgaacaa aaagctgctg tagaagctta tatcgaagag acagctaaaa
     6541 aatcagatct aaagagaacg gatcttgcaa aagaaaaaac aggagtattt actggtgctt
     6601 atgcaatcaa tccagttaat ggcaaggaaa tcccaatctg gatcgccgat tatgtattat
     6661 cttcatacgg cacaggagcc atcatggctg ttccagcaca tgatgaacgt gactttgaat
     6721 tcgctcaaac tttcggacta gaaattcttc cagttatcga aggtggagat gttgaaaaag
     6781 ctgcttacac agaagatggt acgcacatca actctgaatt tctagacggc atgaacaaac
     6841 aagaagcaat cgataaaatg aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag
     6901 tcagctatcg cttacgtgac tggctattct cccgtcaacg ttactggggt gaaccaatcc
     6961 caatcatcca ttgggaggac ggaacagtca cagctgtccc agaagaagag ctaccattac
     7021 ggttgcctaa gacaaaaaac atcaagccaa gcggtactgg agaatctcca ttagcaaata
     7081 tcgaggagtg ggtcaatgtc gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga
     7141 atacaatgcc acaatgggca ggaagctcat ggtattacct gcgttatatc gaccctcata
     7201 acaaaaaaga actagcagat tatgaaaagt tgaagcgttg gctaccagtt gacatctaca
     7261 ttggtggagc agaacatgcg gtacttcatt tactatatgc acgattctgg cataaattct
     7321 tatacgatat cggtgttgtt cctacaaaag aaccattcca aaaactatac aatcaaggaa
     7381 tgatcttagg cgaaaacaac gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg
     7441 atgatgtggt cgaaacttac ggtgcagata cactgcgcat gtacgaaatg ttcatggggc
     7501 cattagatgc ttcgattgct tggagcgaaa atggcttaga aggaagccgt aaattcttgg
     7561 atcgtgtatg gcgtttgatc gttgatgaga acaataaaat gcgtgaccgt atcactacat
     7621 tgaatgatgg caagcttgat aaagtgtatc atcaaacagt gaaaaaagtg acagaagact
     7681 atgaaaatct tcatttcaat acagcaatct cccaattgat ggtctttatc aatgaagcct
     7741 ataaagtgga cgctctgcct tatgaatata tcgaaggatt tgttcaacta ctcgcaccga
     7801 ttgctcctca catgggtgaa gaactatggg caatccttgg aaatgatggt ggtatttctt
     7861 acgcaccatg gccaacatac gatgaagcag cacttgttga agacgaagta gaagttgttt
     7921 tccaagtaaa cggtaaagtt cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg
     7981 agctagaaaa agcagcatta gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag
     8041 tcagaaaagt tattgcggta ccaaacaagt tagtcaatat cgttgctaat taattggctc
     8101 tttgtcaata aggactgatg agttgtgcaa aatcaaatct gggccagaat gaacttcatt
     8161 ctgtctcaga ttttttgtta tttcaaattg attaattgat tgatcaaaaa ggttttgatt
     8221 ttcatgttct caaacacgtt caatgtcttt ttcttctgaa tggtcttgta agagatctct
     8281 gtaatcatca aatttttgtc tattaatctt agtatttttt gatgtataaa ctacctgaaa
     8341 ttgagagtac ataccaaatt cataaatttt ttattagtgg gactagtgat tgtatggatt
     8401 ttgacgcata atgaatgaag tatgagaaaa ggagagacaa atggatcaag aactatttat
     8461 tatcgaagta ttatcagcta aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag
     8521 aataatgttc aagcttttaa gcttagtatt tttctctggt ttgctttttt tcggagctgt
     8581 atcgttccta tatcgccata tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt
     8641 aaataatttg tatcactttt cgtggggagt tggcggaata acaattgttg tgcttattgt
     8701 ttttcttcta cttatttggc tccgttacca tcaattgtcg cgtcatttcg atcaatggct
     8761 tagtcaaaga gcacaacagt ttttacataa taaaccaact tctcaagatg atgactatct
     8821 ttattttgag aagacttcga cgagtcaagc ttttcggata caaaaaagtg attgccagtt
     8881 attgctgaga tcacttgatg atttacctat ctacttaggc gaaagcaaat caaaattcgg
     8941 aatgaacaca ttccatattt ttttactaga aaacgaaccc agatgggaaa aaagccagtc
     9001 ttcgcctggt ggatacctga caactaagat gagtacatta gtcactggtt ttttcctcat
     9061 tcttttagct ggaacaatct attatgggag atacactcag ccaaaaatgt cttctcatat
     9121 aaatgctgct tctcacatga caatgaactc aactaacgat acgcatatac aaaagacaaa
     9181 tgaagggttg ttagctgatc ctaagcaaaa ggtaaatttg acaacacaaa agataaatga
     9241 tctcaaactt gatacagaga cgcactctct ctatatgaca acagatagtg gtgctaattg
     9301 ggtatttgtg ccgattgatt cgcaatggtt gcgttttggc gaatacaccc tgacaacggg
     9361 gctgattcct gaaggttatt ggatggacaa cacatatgat atttccgccg atttctcatg
     9421 gtttatctat tcttctgggg atgatgtacg tctgctgact tcaagagata atggtaaaac
     9481 atggcaaacg tctattatca cccaatcagt tcagccgata cgttatcgaa aagcggtatt
     9541 ttatggcaac gatggtctat ttatctgtac atcagccagt gggatgtctg cagaaacttt
     9601 gtacatttac actactatgg atggcggaat cacttggata aaaagtggaa gtacaatgat
     9661 caatcagcca gtacaaaatg tcagcttcgt tacaacttct ttaggttttg tttcgacacg
     9721 tgaaagtttg ttttatacaa acaacggagg acgctcattc aaagaatcag ttctgtcgat
     9781 tccggaaaac tatcagacag gggggataga tctttttcaa tcaccaaatg aagttactca
     9841 aataagcgcg aatacgttag agacaaaatt ttatttgttg aaaaaaggac caatcgatgc
     9901 agggaaaatg tttgcctgcc tcttccgttc aactgataac ggggaaacat ggcaattcgt
     9961 tcagcaacta tcgcaagtag aacaaggaga ttaaagaaga gagattgtga gcctgacgtt
    10021 cagcaccgag tcacaagaaa caaattcaaa aaacagcttc tcatatttgt tgaatagtgt
    10081 aactcgtgat tcggagttga caagagcaac aagaagcgat tttgaagtat agcttctcgt
    10141 attttacaag atcgtgtctt attgccgata ggttgtctct tgaacaccgt ttattcgtaa
    10201 aaagggattg tgacaagtct tttgtcacta cttaccgaac gaaactgaag gagctatcca
    10261 aaaagtatca ttagtattat tctttgtgtt tctagtggca ccactagtac atttaaaaaa
    10321 gctatcgatc atggactaat catacatgat cgatagcttt ttagataatg tttgttacgc
    10381 tatctgaaat tgactattat aaagactggc ataaagcgtt ggtgattgga gtagttcaga
    10441 atgtgttcct ttttcgacga tatcgccatt cttcattaca aggatcatgt ctgcattttc
    10501 aatcgttgac aaacggtgtg caatcacaaa acttgtccta tcttttgtca cagcttccat
    10561 tgcttgctga atcaatgctt ctgttcgtgt atctacgcta gaagttgctt catctaaaat
    10621 aacgacagga ggattcgcta gaatgactcg agcaattgtc aataattgtt gctgtccttg
    10681 agataacgtg ccgttttcgc tagaaatgat cgtatcatat ccgtctggca gtgttcgaat
    10741 aaagtgatca cattgtgcga tttcagccgc ttcgataatt tgttctcttg tggcatccat
    10801 tttcccgtag gcgatattat cagctaccgt tccttcaaac agccaggtat tttgtaaaac
    10861 catcccaaaa gaacgacgta gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa
    10921 ttcgatcttt cctttattca gttcataaaa acgcatcagc agattgacta aagtcgtttt
    10981 tcctgcacca gttggaccaa cgatcgctac cgtttgtttt ggctttactg aaaaatcgac
    11041 ttgattcatc aatattttat ctgatgtgta accaaactgt acatttttga aagctatttt
    11101 tcctttcggt tgttctaaaa gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc
    11161 taatatctca aatatcctat cgattgctgc cattgcggat tggatcgagt tgatcacata
    11221 agaagcggta gaaattggtt cagaaatctg attgatgtac tgaagataag cttgtaagaa
    11281 gccgatcgtc acaccacctg atagaacaag catagcaccg aataccgcac ttatgatgaa
    11341 ggcgagttga ttaatgaaac gaatggccgg atagatagca cagttcataa attgagcacg
    11401 tttaaaagcg ttgtattgct ttttatttac ttcgtgtata gcagcttctg catgagcttg
    11461 ctgattgaat gttttgacca ctagattacc agctaagatt tcttccattc gattatttaa
    11521 aacacccagt tcatactgat tctgttcaga aatctgtttg tttttgttcg caatcttagt
    11581 tgtaacaatt gaactgccaa cgatcagcaa taagacgagc aaagtaagct tgctttcgat
    11641 ataaaacagc atcagaccag cagcagctat tgttacgaca gaagtaaaaa attgattgat
    11701 acctgtcaat aacacttgag aaagttgatt gagaccggta gttgtccgac taatgatgtc
    11761 acctacttga tgattgtcaa aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc
    11821 ttttcgcacc ctcaatgtga tcttttcgct taacgctgcc attgtccgtt cctgaataaa
    11881 tgaagtgatg ctgcttaaga ctgaacagcc gatcagcaat aagacaggga acaacaatgc
    11941 ttgtttgata tcactgaaag aaagctgtcc aatacctgtt tttcttattt ctgataagag
    12001 atcatcgatc ccgattccca tgatcaaggg catggctacg actaataggt tcccgaataa
    12061 actgcataga aacaatccgt aaaaatattt atgttcaggt ttgatcattt tccaaaaacg
    12121 tatcaaagca cgcgtgttca acgttttttt cttttccatc gtatcccttc tttctatatt
    12181 ccttgtgatt ttgcaaactc ctgataagtt tggcaattag acagtagttc ttcatgagta
    12241 ccttgtccaa cgatttttcc ttcatctaaa acaatgatgt tatctgcttg ttggattgta
    12301 ctgaggcgtt gagcaacaat cacgagagtt ttctccgaca tgtgtttatg aagggctgct
    12361 cttaatgctg catctgtttt atagtctaaa gcggagaaac tatcatcgaa tacatagatc
    12421 tctgctggtt tgatcaacgc tcgagcaata catatccgct gtttctggcc ccctgaataa
    12481 ttagaaccgc cttgagcaac aaagctgtcc aatccgtcag gaagggtgtc cactagttct
    12541 tctaattgag cgatcttgat cgctttagac aactcttctg ttgaggcata ttcatttccc
    12601 atctgcaagt tgcttaaaat tgtcccgctg aataagaaag ccttctgagg gacataactg
    12661 atgtgggagc ggatctgttc ttgagtcatt tgagtaagag gaataccacc aagggttact
    12721 tttcctatcg tagggtcact aagtctcaag agaagtttcg ccaaactact ttttccagat
    12781 ccggtcccgc caacgatcgc tgttgttttt cctttaggta ttgtgaaatc aatatcttct
    12841 agaactggat cagagatttc agtatattta aatgtgacat gttcaaaagc agctgcattt
    12901 gtctgatcaa aggttggttc agctactgtt ttgatcgtct cttcctcaat ttcgatctct
    12961 gcttgcagta tttcttctat ccgtcgtaat gaggcaaacg atttagggat aatgaccaat
    13021 accatagctg cgatcatcaa gtaagcaagt gtcagcatag agtattcgac tacagcagtc
    13081 acgacaccga tttgtaatgc tccttctgct acaagatttc cgccaaacca caaaatagaa
    13141 gcataaacga ttcccatgag caaaaaagca attggagtga cccaagcaaa tattcggttc
    13201 actttgatca tttgattagc atagtcagca aagctcttat tcgttcgctt ttcttcatat
    13261 gattgattat caaaggcacg gatcatatta atcccagtaa agaattctct caatgtgatg
    13321 gtgatccgat ccatttttgg ctggatactc aaagataaag gtgcccctcg tttcatcagt
    13381 cgataaacaa tccatacata aaaaacaaca gagatcaatg taacccatgt tagtaataga
    13441 gaataacgaa acatcatgag aatggtgaaa acgcaaataa ccggtgctgg taagatcatt
    13501 tggcaaaata aaacaatcat ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa
    13561 agagatccag aactaaaacg actgatatcc ttcattgaca gctgttggaa tttacggaaa
    13621 tatttctccc gcgtttggaa tccaaaccta gcggaagcca atgctgaaaa gtaactagag
    13681 gcaactgcag cgattgttcc tagaaacgct acaagcagca tttcaactcc aatttttttt
    13741 ataactgagg cttcgccact tgctatacct gtgtcgatca gctttgctac taattttggt
    13801 acacctaatg taccggcgat ttctaaacag atcatcacaa aggtaaggag agcgagtcct
    13861 ttgtttttct gtaagaatgt ttttaagata gacatattat tcctcctttt ttcctgatac
    13921 ttttcgattc tataccttca agtaacttga aggtcaatag aaaaaagaat aaactgaaaa
    13981 agaggtgaga aaaaatgaaa aaaatgtata cgattggcca agtatcggaa ctatttgatt
    14041 tgccaaaatc aacgatccgc tattgggatg aacaaggatt aatccgttca agtagacaag
    14101 aagagaatga ttatcgattg tttgatatcg atgatatttt tatgctttat gatatcgttt
    14161 tttatagaaa attagatata ccgatcaaac agatgaagaa tttatatgga aaaacattga
    14221 cggagttata tgagacactt gatgaaacag aaagaagaat ccataaagaa ctggtggtga
    14281 tgaaacaaaa acaaaaagag ataagggagc aaaaaaaaca gttgaaattg atgatcgata
    14341 caaacgagga agaatttcct gtagaagaga tcccttttga ctgtatgatc tcaacggagt
    14401 ttgaagatat tgtggaaatc aaaaaatttc tacctaacta ttcaagcttt gggatgatga
    14461 gtatgccagc ttccagttca cagacgatgt atggattttt tatcgaccct agtgaagtac
    14521 atttatttac tcaagacgtg atttgggaga aaaaagatac ggctgtttat cgtcgattct
    14581 tattgaaatc cgagatgaac catgcggaac gtaataatat tttagaaatc agagaaagaa
    14641 tgtatgaaaa aggatggaaa acaggagaag tgatcggcca gtatctattg acgaatacgg
    14701 atgaaaacaa tatcagaact gaatattatc atgcatggat agaaatgaaa aaataggaaa
    14761 tatgtgttta agataactcg aaaatgatgg ttgttttagt tataaagaaa aattttctgg
    14821 ttcgattacg ggtttaatgt gggggattat tagtcgtgga tagatagaat tttagttttt
    14881 tagaggaaat atgtctttaa aaatggaatg gttgcaattc ttagcgatat atacgatgta
    14941 attgtcctct taaaaaagta tggaaaaata attgtattgg gtgttatgta taattcatgg
    15001 tgaactgaga ctaatgagaa gaaaaattca tattgttatg agtgagattg tatcagatga
    15061 gaatgataaa ttaaataggt gcataaaagc attacactta aaatacattg ttgcttacat
    15121 tattacaata tgttaataag gcaaaaataa atagatggat tgaataaaca tattttttaa
    15181 tcaaatagtc cctactgtga ctttcctcag ctatcggtga tactaccgat agcatttttt
    15241 tgcttataaa ataattataa gtatttagag atttttcttc taacattctt caaacaagaa
    15301 aaatcacaaa ggttattgtt atttttatat attcttttta agaatgattg tttttttata
    15361 gaaattttaa aaaataaaac acgttattga tggatagtta gtttgttatt tggtaatatt
    15421 atagaagttg actggtagtg atttattttt cgtcgtttta taaaaatgtt attttttgga
    15481 aaaattcaca aatatgtaac gaatgttttt ctggaatttg ctgtgttaaa aagcagtatg
    15541 actaatataa atactattgt catttatgca attttttatg gatagtaatt ttagagggga
    15601 atcaatgaag gaagaataga agggaaaaat agttgcaaat acgccagtta gcaaaaatag
    15661 caatacatct tcaagcttta aaattcgaga gaatcatctt ggtagtggaa tcaactacaa
    15721 caaaaggatt aaaccaatta atagatagaa ttaccaatgg aaaaatagaa aaggtagtta
    15781 ttctatacaa aagttgttta attcgcttcg gttatgaact tagtgaaacc ctttgcaata
    15841 tatggtacag aatatagaaa ttattgacat tacagaaaat gatgaagatt taatacagat
    15901 tgtcactatt tgtagatacc ttttacaagg aaaccgagcc aataaagcta agaagatgat
    15961 taaggagttg atggaagacg atacaagtta agaaagtgaa actacgccca aataaagagc
    16021 aaaaagagta attggacgtg gaaaatgcaa gaaatgaacc atcgattcgg tggacagttt
    16081 attagtaatg acggtttgag aacatccatg acgagaatga aggaacatcc taaatgcgtt
    16141 tagttgaacc aagtgccaaa taatatggcc aaataagcga tcagttatcg atgaatccca
    16201 agtattctaa actatatatt cattttgatg ctaagcattg atatattttt ttgggattga
    16261 tgaaggttaa ccaatacagg gactcactga tgaaattcta gagtctgacc ttggtatcaa
    16321 cgatttagtg attgtttcta atggaaaagt ataaaagtat ataagtacat taacaaaata
    16381 actattaggg ttacgctcca gcttgtgcaa ggcactggca ggaggaatcg tgaaatcata
    16441 attatggtta agccacacgg acaactaaaa gtcatacaaa gctatcgaat caatcaacag
    16501 aagacaatcg gcaatcatta gctttgttta cagcttacat tgcaacgctt gagttgaacc
    16561 gaaaatacga gggaggtaac gcagccattt tgagttgggg aatcttttta gaaaattatc
    16621 gggtaccttg ttgtttagtc aaaattataa gggggagtaa tagtgaggaa caaatttcat
    16681 aaactgttaa gtatgtttct agttatgctt attgtgatca atcaatttgg gaacataacg
    16741 gttctagctg agtctttagc agaacagcca gagcgttcgc tgacgatcga agttgatcaa
    16801 gaggaggctg ctttgggtga gcagttccag ttgcgagtca gaggaacacc agacctgcta
    16861 aatgcattgg tcattgataa gagtgaggat ttgattgaag gggaagtgaa gcaactcaat
    16921 gattacgaac gtctgattac ttattcttcc cagcattcag gtaaatacga attgaccgca
    16981 agaagcggtg atattcaatc aaatacggtc tcaatagaaa tcagtgtacc agaagaaagc
    17041 acaagtgagt cttttaatgt accagaagaa agcacaagtg aatcttttaa tataccagaa
    17101 gaaagcttaa gtgaatcttt taatgaagcc agatcatctg agccatctat aacgcaggaa
    17161 tctcagattg aaagacaaga ggagctcttg aattatgaca tcggcatctc tgctgagcgc
    17221 cccactttgc cgagcaggca tacacaaacg ttaaagggat acattcaagg aggcgctggc
    17281 gatattcttg aagaatatgg ggagatggtc gtacgagttc ctagagcatc gattggctta
    17341 attgagttca ttagagtgga ggtcgctgat gattcacctt taaagccact ggcattttac
    17401 caagaaggca cagacgttgt ttttccattt acctttgatt atgaaggatt aaatgaagcc
    17461 gctcagtcgt atacattcag tatcacgttt gaagttgctt tctttcaatc aggcggtact
    17521 gagccaaata atgactttca agtgagtctt acttatgctg agcgagcagc aaacacggaa
    17581 ttttcaatta cgcctattcc ttcatcaaga cccatttttt cgaaattata ctatggctct
    17641 agagaaatcg acagccaagg aaatcttggg aatctgctga ttgacgggca agacgtgacc
    17701 aacagtaata aatttatttt gcaggtaaat tatgaaggat cacgaaaaga cggtgtagtg
    17761 gttaccgatg tactaccgca aggaacaact ttggcaccga atccgactac atggagttcc
    17821 actggaaata gagaacctat ttcaggtggg gttcggatcg cagaagtaac gaatccaggt
    17881 gaagcaaacg ttagaactca ttatgtttcc gaggaatttg tggacaaagt atcttttaat
    17941 aaggaaacga atacacttgt tgtggacttt ggtaatctag cagagggtca atcgtatttt
    18001 attgaatatg ctcttgaagt gaccgatgtc gacttgttca atgaaggaag acaagaggat
    18061 gggttttctc ggaatcgagc gagtatgacc tacaataatc aggtaatgag ccgagactac
    18121 cgcttgagaa tccctgaaag taatcttccg gcaatctctt taagaaaaag tgtggacaaa
    18181 aatgttatca atctcaatga aaatgaacta acgtatactc tgcatgtctc gatgttttct
    18241 ggaacaagaa atacgcttgt tattgaagat gttttggaag aaggcatgga atttgtgtca
    18301 tttctatcag atattccctc tttcattgat gccgaagtca ataatggaat agttcgtttc
    18361 agtgcgaata atacgattgg tggtccgaca agttttaccc tctcttttaa ggtcaatgta
    18421 gaggcgtatg cggtcggcga tcaaatttta aactttgcaa cgcttgtgaa tagtgacaat
    18481 cgattgaata cgagtactgt aagaacaagg aaaatcgatg ggcgtattaa agtaattaaa
    18541 actgatgaga atggccaaag gctggcagga gcaaaatttg aaattttatc aagcgattca
    18601 caagtgatac aagaaggtat aacggcagaa aacggagaat ttttaagtga accgcttcct
    18661 gtaggtagct atcaagtccg agaaacagaa gcaccggaag ggtatctttt agatgcgacg
    18721 gtacatgatg tttttataaa tgaatactcc atagaagaag tgacgttaac cattgaaaac
    18781 atccttgata cgggtggtgt tgaactaatt aaacatgctg aaggcagtgg tgaagtcctt
    18841 caaggagctg tttttgagtt acagaaccga gaaggagaaa cgctacaaac cggtttaact
    18901 acaggagaag atggaaaact ggcaattgac ggtttagcac caggagcata tcagctggta
    18961 gaaac
//
LOCUS       DACGLH010000054        17812 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.058, whole genome shotgun sequence.
ACCESSION   DACGLH010000054 DACGLH010000000
VERSION     DACGLH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.058"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            181..882
                     /locus_tag="IXT21_001968"
     CDS             181..882
                     /locus_tag="IXT21_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869353.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            892..3603
                     /locus_tag="IXT21_001969"
     CDS             892..3603
                     /locus_tag="IXT21_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ4869354.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            4054..5682
                     /gene="treC"
                     /locus_tag="IXT21_001970"
     CDS             4054..5682
                     /gene="treC"
                     /locus_tag="IXT21_001970"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ4869355.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5742..6923)
                     /locus_tag="IXT21_001971"
     CDS             complement(5742..6923)
                     /locus_tag="IXT21_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4869356.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            7175..8698
                     /locus_tag="IXT21_001972"
     CDS             7175..8698
                     /locus_tag="IXT21_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ4869357.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(8767..9495)
                     /locus_tag="IXT21_001973"
     CDS             complement(8767..9495)
                     /locus_tag="IXT21_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4869358.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9508..10788)
                     /locus_tag="IXT21_001974"
     CDS             complement(9508..10788)
                     /locus_tag="IXT21_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4869359.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(10929..11531)
                     /locus_tag="IXT21_001975"
     CDS             complement(10929..11531)
                     /locus_tag="IXT21_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ4869360.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            11776..11982
                     /locus_tag="IXT21_001976"
     CDS             11776..11982
                     /locus_tag="IXT21_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869361.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            12294..13013
                     /locus_tag="IXT21_001977"
     CDS             12294..13013
                     /locus_tag="IXT21_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4869362.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(13094..13777)
                     /locus_tag="IXT21_001978"
     CDS             complement(13094..13777)
                     /locus_tag="IXT21_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ4869363.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            13995..16181
                     /locus_tag="IXT21_001979"
     CDS             13995..16181
                     /locus_tag="IXT21_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ4869364.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATVKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            16527..16937
                     /locus_tag="IXT21_001980"
     CDS             16527..16937
                     /locus_tag="IXT21_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ4869365.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            16951..17307
                     /locus_tag="IXT21_001981"
     CDS             16951..17307
                     /locus_tag="IXT21_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ4869366.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         6367 a         3007 c         3450 g         4988 t
ORIGIN      
        1 tccttctttc aaataacaat gttttttatg taagtaaatc ataaatcgtt tatttgtcaa
       61 ctaaattaaa ggattccttt ctttagtcta gagaaattca gtggtaaaat aaataagtgt
      121 gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg aggaatcaga
      181 gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga aacgaccatt
      241 acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat tatacttggt
      301 ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga tacacctgat
      361 gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa acagcttact
      421 gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt tcctaatcta
      481 acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct tgatcctgta
      541 gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc ccaattatca
      601 ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc caaacttctt
      661 ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt attgaaatta
      721 ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca taattctgct
      781 ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag gtctgttgaa
      841 ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt tatgaaaaca
      901 agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg attcattgca
      961 atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac tgctggacct
     1021 gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag gactttctga tttgcaaatc
     1081 gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat cccagatgca
     1141 aaagtagaaa caggaaaaca gatctattat tccaatactg gcaaaaacga agtgattcaa
     1201 atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga tggaaaactc
     1261 ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg gtataaaatc
     1321 ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac catcacgggc
     1381 tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa cgtcggaaat
     1441 ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga tatttattct
     1501 gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa agcttacaaa
     1561 gataaaatgg agcaaaatca agaaaaggca gaaaagtatt tacaagatcg acccgaagaa
     1621 cgcttagaag agctgaaaaa atcggccaac gaagaattag aaccggcaca gcaaaaaata
     1681 gcggatggca aagcacaaac aacgcaagca agaacacggt tagaagatgc taaaaaacag
     1741 ttagcgcaac agtcagcaac tatccagcag cttcctgaag cgcaacgaac aatcgctgtt
     1801 caaacattat ctcaacaaga agctcaactt gctgaacaag aaaggcaatt agttgagaaa
     1861 gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa aattgaaaat
     1921 ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt tcaagaattt
     1981 ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt ctttttcttt
     2041 attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa ccgcagggaa
     2101 atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa atatgcaatc
     2161 tatgcaagtc tagcctcagg gataggtatt gttttaggaa cgatattagg aacgaatctt
     2221 ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag tgcgcttatt
     2281 ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc cgcatttggc
     2341 gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt gcttcaaccg
     2401 aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct gtggtcacgc
     2461 cttagcttta atcaaaaaat cagttacaga aatctcttcc gatataagtc aagaatgctt
     2521 atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt gggattgaaa
     2581 gattctctaa gttctgtttc agccaaacaa tttggaacga tcattgacta tcaagcaatc
     2641 gttacattgg attcatccac ggaagatagt cgtcaaaaag cagcggatat gctgcagaag
     2701 caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt gagaaaaaaa
     2761 ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga tttggatcgt
     2821 tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac aggaatcgct
     2881 attacgcaaa aagtagcaga actatttgac ttatctacag gagatcatat ttcgctttat
     2941 gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa ctatctaggt
     3001 agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc gatgatggat
     3061 aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat gctttccgag
     3121 aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca aatcgaaacg
     3181 caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt attatcagga
     3241 ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga gcgtgtacgc
     3301 gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat gtacattgtt
     3361 cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt aggatatata
     3421 ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc attagtgatc
     3481 acatggtggg cttatgtctt atcagcagga ttaactatcg tatttacggt tattgtgatg
     3541 attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa atcgaatgaa
     3601 taaaaacaga ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggcaaa
     3661 atagtttttc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
     3721 atgaatcaga ggttgtaaaa aagccgtcct gcatcgagca aaaagaatac tcaaataaaa
     3781 ttagctcctc atgttttaca tttgagaaga ctgattgccg tagataaaag cttttgaaca
     3841 ccatttatag aagaaacaag aatattttgc tattttaaac aagaggatag acaaatacat
     3901 gtctgatctc ttgtttttta ttgggcaaca gacctcttag aatgtataaa gataaatttt
     3961 acaaacgctt tcggttcagt tataataata aatgataact tgtatatata agttgtggta
     4021 ggtaataaat cagttgaatg gaggtaaata tccatgacat ttaaagacaa agtcatttac
     4081 caaatctatc ccaaatctta caaagacagt aatggtgatg gaattggaga tttaaaagga
     4141 atcaaagaaa aactcccgta tctgaatgat ctaggcatcg acatgatttg gctaaaccct
     4201 atttatccaa gccctcaaaa agacaacggt tatgatatct ctgattacac agccatcgat
     4261 cctatctttg gtacgatgga ggaattcgaa cagctaatag ccgaagcaaa gaaataccgg
     4321 atagaaatca tgctcgatat ggtgctgaat cacgtttcga cagaacatga atggttcaaa
     4381 aaagcactag caggggacca gcattatcag gacttcttca ttttaagaga agagccaacg
     4441 gattgggtat caaaatttgg tggaagtgct tggtcgccat ttggagatac ggggaaatat
     4501 tatttgcact tatatgatcg gacacaagct gacttgaact ggcggaacca agcagtaaga
     4561 gaggaactat tcaaagttgt tcgtttttgg atggaaaaag gggtaaaagg attccgtttc
     4621 gatgttatca atgttatcgg taaagacgaa caattgaaaa ataataaaga aaatgatggc
     4681 aaacctgaat atacggacaa accaatcacc catacgtatc taaaagaatt gaaccaagca
     4741 acgtttggga cggattccac tatcatcaca gtcggtgaaa tgagctctac gacgatcgaa
     4801 aattgtatct tgtatacaga acctagtaga gaagaattat caatggtctt taatttccat
     4861 catttgaaag tcgattatga aaatgggaat aaatggtcta ctcctccatt tgattttgca
     4921 gaattaaagc gactatttca tacatgggga gaaaagatga gtgagggtaa cggctggaat
     4981 gctctatttt ttaataatca tgatcagcca cgtgcgctca atcgatttgt agctatcgat
     5041 cagtatcgaa aacaaggagc agagatgcta gcaaccatga tccatttgaa ccgtgggacg
     5101 ccctatattt atatgggaga agaaatcggc atgatcgacc cagactattc atcgatttcc
     5161 gactatattg acgtagaaag tttgaatgct tataagttac tccttgaaca aggattcaca
     5221 gaaaaagaag cgtttcgtcg gatcaaagca aaatctcgcg ataactctcg aacacccatg
     5281 caatggtcag acacagaaca agcaggattt actacaggga aaccttggtt aaaagttgga
     5341 ggcaagctag aagagattaa tgtagaaaaa gaactatctt ctgaagattc tattttgtcc
     5401 tattacaaga aattgattcg gctgagaaaa acatatccaa tcgttgcaca aggagattat
     5461 catgcttatg aagcaaatca tccggaagtt tatggctttc ttcgccaatt tgaaggacag
     5521 cagctgcttg tattaacaaa tttctatgat aaagatacaa ggatcgccat cccagaagga
     5581 ttcacagaag ccaatattct tatcgataat tattccgata ctgttcttca tcaaagtctg
     5641 gagctaaaac cataccaggc aattgctttg ctgaaaaatt aaaacaaaaa cacaaaacgt
     5701 cgttcctacg agctattctc gtaagatcga cgttttgtgt tttatttaaa ataatcttct
     5761 tcagaaaatg aagagttttc atactctcct ttatcgccat aaaatttatt gtagcgttgt
     5821 ttgaatttac ggaaaataaa agtaacgagt aatttgacta acgtatagat aggaatacca
     5881 aaaataacgc ccacaattcc cattagatca cccatgacaa gtaaaacgat taagatcgta
     5941 atcggatgga tctgtaaacg atcacccatt actcgaggaa tcaccaagtc accgtggaga
     6001 agttgtacgg caaaccagac aatgacaaat ttgacagcca taaaagtaga gtcttggaat
     6061 gcaatgatca aacctggaaa gaaggcgata aacggcccaa tatatggaat gatacataac
     6121 aacccagcag aaatagctaa aacactagca aattctaatc cgatcagtaa gaaacaaacc
     6181 cagtagactg ctccaaggat aaatgaagca atgatttgcc cctttagaaa tgaacccaat
     6241 tgttgattag caatctttgt caaattttta aaatctgtac gaaatgctgg tgggacaatc
     6301 gctaaaactg actgataaaa tttttgagga tctctcaaaa ggaaaaaagc gacaattgga
     6361 ccagtaaata aagtaataaa aatagctgta acagttgaaa aaatgttgct gatccgttgt
     6421 gcgccacttt cccaataatt tccaataaat ccagctatat cagacgtcac gtcgtcaata
     6481 gaagcgaaaa attgattaaa tgaatcggca aaaggtgttt tctccaaaaa ttgtcggaga
     6541 ctctcttgaa aatcttgaaa caaatctggc aaactactga tcaatgtctt tgcctgttct
     6601 tcaagaaaag gaataagcca tatgcttcca agccccaata aaaataaaat aatcacataa
     6661 acgataatga ctgacaagtt cctcgaaagg tgcttgctca aacgtttgat gactggttcc
     6721 aaaagataat acaagatcag tgcaaacaat aatgggggca agatagtagc gataatgatt
     6781 ccgacaggct tgaatataaa cgagatctga tggaaaaaat aaatgatcag cgcaagtaag
     6841 ataactattc ctaaaacata gtaagaggtt tttcctccta aaaagcggat caaacgattc
     6901 gttgtatctg tttctttttt cactcttttt cctcctttat ctataacttc attatagaca
     6961 aaagcgggta taaaaggaaa atgatagagg ttgattgcaa aaatatcgtc aatcattcat
     7021 ataaactttc tcatcaatta aaataagaaa acgcttgaca gttcttttaa ccatatgata
     7081 aattaaatcc tgttgctaaa acgtaactat tacggtttgc gaaaagcgaa tcggaacatt
     7141 caaatgaaag aagatgagaa gaggagtgtc ataaatgaaa cgattgatag gtatattggc
     7201 catgctggtt ttagcaggta tgttaactgc ttgcggggca agtgggaaag ccgaggacag
     7261 taaagaaaaa ttgagcgtga tgacgacctt ttatcccatg tatgatttta caaaagcaat
     7321 cgtcggggat gaaggagaag tagagttatt gattccagca gggactgatt cccacgatta
     7381 tgaaccttct gctaaagaca tggcaaagat ccaagataca gatatctttg tttataatga
     7441 tgaaaacatg gaaacatggg tccccgcaat ccaaaaaaca ttacaagaag gcaatgtcca
     7501 tacgataaaa gcgacagaag gtatgctttt acttccggga agtgaagaag gacatgatca
     7561 tgaccatgaa cacggggaag aaggccatac acatgagcta gacccacatg tatggctagc
     7621 tccaagttta gcaatcaagc aggtagcaaa catacgtgat cagctgattg aagcttaccc
     7681 agaaaaacag gaagtatgga caaaaaatgc agcagcctat accgagaaac ttcaagcact
     7741 ccatcagctt tatcaagaaa cctttaagca agcaaaacaa agatcttttg tcacgcaaca
     7801 tgctgcattc aactatctag cgttagaata tggattgaat caagtatcga ttgctggtct
     7861 ttcttctagt gaagaaccgt ctgcggcgag aattgctgaa ctgaaacact ttgttaaaga
     7921 acatggaatc aactatattt attttgaaga aaacgccaaa gatagtattg ccagaacgtt
     7981 agcaaacgaa gcaggtgtat ctttagaagt attgaatcct ttggaaggat tgacgaatga
     8041 acaaatagaa aatggagaaa actatctttc tatcatggaa gcaaacctcg aagcgttgaa
     8101 aaaaacgaca gagacagaaa acccgctaga agatactttg acccccaaaa aagaaaagag
     8161 cgtctacaac ggttattttg aagacgagga tgtacaagat cgctctcttt cagattgggc
     8221 aggaacttgg caaacagtag atacgtatgt agaagatggg acttttgatc ctgtttttga
     8281 atataaagaa aaattgaatc aggataagac agcacaagaa tataaagaat attatacgaa
     8341 gggctatcaa acagatattg cttcaatcaa gatcagtgag aaagagatgg catttacgtt
     8401 taagaatgga acaacgaaac gttcaaatta tcgttatgta gggaaagaaa tcttgaccta
     8461 cgaggcagga aaccgtggcg taaggtatct attcgaagca gaagataagg aaagtggcgc
     8521 ttttagatat attcaattca gtgatcacac gattgcaccg gctgactccg aacattttca
     8581 tttatatgca ggaaatgaaa gtcaagaagt attgctgaaa gaaatggaga attggccaac
     8641 attttatcca gatacaatgg atggacgtga aattgcacag gaaatgcttt tgcactgata
     8701 ggcaaaaaag aaccgaaggg tttggatcgg ctggtcattt agtcggtcca aactattcgg
     8761 ttctttttat gaaatcaatt tctttaatcc acggatagag gctgggatgg tctcgccgtt
     8821 tgccaacgtg acttctctgg tttttttatt aatcgaccgc acattttgtg tattgataac
     8881 ataggattta tgacaacgga taaaattccg atgcattttc tcgatttctt tgatcttctc
     8941 atagaattgt aattgggcat gagtcaaatg gacttccagt ttatgtggcg ctcctagcga
     9001 agtagaaaaa tattggatgt ctttgatcgg taaaaatttg atctcatcat taaactctat
     9061 tttgaagata tctttttgga tttgggacat ttgaaaatct tcgaaaattt tgctcaggat
     9121 atgctcgaca ttttctttta aatcataaag atcttctttg acgatataat cagtcggttc
     9181 gatgttgctt tttaagattt tcatcgacat ttccgtatgg gaagtcacat agattatttt
     9241 tcctaaagga tctaattcac ggatcaattt accgatcgct acaccatcca tttcactgtc
     9301 ttctagttcg atatctaaaa agtaaatacc aaaagaagaa gttctgatct tggctgcatt
     9361 gagaagttct tccggatcag aggtagaaat atggacataa ctgtctaagt tttcaatcat
     9421 gattctattt ttgatcaccg ttgataaaac ttctaattgt tttggatcgt cttcacaaat
     9481 ataaatggga atcatatcat tactccttta ttttatttgt atataaatcg tttgagaaaa
     9541 acgctggttt tcaattgatg tttcaaggaa aagataagga ttggtctttg tcaattcatc
     9601 caagataaac aagccaaggc cttcgttttt tggatttgtt tttgttgaga accctttttg
     9661 tttcaactgg tttaacggta atgtatttgt tgttgtattg atcacacgaa tcatcacgga
     9721 atgattcttc ttgagtatgg tcaattcgat ttttttcttc tttgcagcac tagctccttc
     9781 aatcgcattg tccaagataa taccaagcat acgtacaagg tttaccgtat gtttcgtatc
     9841 cagttcgatt ggctcaggaa aatgaagctg gacgatcaac cctttttcct gtgccatcat
     9901 gagttttaat gacagtaaac tacggatctc aggaactttc atattatcca acatacttag
     9961 attggccgga aaaaggttca tcatctggcg tgtaggtaat atcgtttgat cataataaga
    10021 ccgtaatcct tctaagtcgc cttctgaaat atagctgtga agagaaaaaa gcagattttg
    10081 gtaatcatgt ttgaattctc tcaagtcttg atagtttttt tctagatctt tgatataatc
    10141 ctgttgcatc ctgacttgtt gctctaataa ctgatcagct aatttcttat tagagatttt
    10201 ccaataataa accaagatga tcaggccaac acacagatat agaatgaaaa aactcgtatt
    10261 caaagctaag acatcaggtg tttcaccaga aaaacgggta taaaaaataa tgatatagta
    10321 tgtaaataat atcaatgtcc cgatcgtacc gatcatacgg cggtccacag tgtgaaacca
    10381 agtctctaac gattttttta aacaaaaagc taataaagca gataaaaaag cggataagac
    10441 aatgtgaaaa tactgcaatg aatctcctgg ttcaataacc gaagatttca aaacaaaaaa
    10501 atcgccgatg cttgttaagt gatctcctaa aagcgacgtc aataacccaa aaggaagcgc
    10561 gtaaaccaat tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca gcattcctat
    10621 agcaattaga gcagtaaaaa tccctaaaat cgaatagacc catccaatca aaatacaaga
    10681 aatcagaatg actcccgcaa acagatattt gtatcggatg tgatggtcaa tcaataagat
    10741 acaaattgtg taaatcaaat attgcaacgc tatggaccat aaaaacataa agtacagctc
    10801 ccattgattt ttaccaaatt atagcacaat tggcgtatta tttttttaat ttttcaggga
    10861 ttttgaattc agaactcata ccaaagcagc agccagatac aaaaaagtca gataagttag
    10921 ttaattgttt aatgattgtt tttttcatga aagattcctc caaaattcat caagtagttt
    10981 gtaataggaa gcaacgtagc tatttctaaa acaatcccaa gtagcaaata ggtagaaaag
    11041 taagaagctg agaaaaataa cgtaatgaga gaaaaaatta gaaacatgct gatcgccttc
    11101 tttaataatg ggggacgttt ctcgggttca atgggattga ttgctgtacc tcttgatcca
    11161 tagcgaagca gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg gaaggagata
    11221 gtcaattgca gatgtttcat caccaaggga acgccccata aataaagtag tgtccacagc
    11281 agacaaacga attcagagtc ggaatgcaat ccataagcat atctgcggat gcagatataa
    11341 gaaagaaagg tgattcctgt ttccgcccat gagcctgtca ccagagaaaa gactagaacg
    11401 accgctagtt ttccaagatt attcaataaa acttctaaga caaattttac ttggacatac
    11461 accatatctt cttccgattt gtctccgctg aataaggctt ctaataattt gttcacaact
    11521 ttatctttca tctcatacgt acctccacca caaattttat gctaaaaaaa ataaaaaacg
    11581 tcttttgttt cataaaaagt ggttttgaca tcaaaaccgt catgatctcc taaaaaaatc
    11641 ttcgttttcc aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa gtacttttta
    11701 tgacaagttt gtgctttttc gagtgattta tgttaaaaaa ttactgacct tataaaaggt
    11761 gatggaggga ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac atatgcattt
    11821 aaatctgatt tatttgaatc accaataaaa ggaaaaatag aaaaaatcta tgaaaatagt
    11881 gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg ataccgctca taatctgcac
    11941 tggcagttag ttgtacgcaa atcaagtaca atagaagaat aaaaacagat aaaatgttga
    12001 gagcataaag aggttgtgag tctcccgttt agctctgagt tacaaggaat aatgttaaaa
    12061 aacagctttt catgttttat gaatattgcc ttgtgaccga ggtgttggca ggagaatact
    12121 gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt tcatgaatag
    12181 gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt
    12241 ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga
    12301 taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacacta actgaaatgc
    12361 ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc
    12421 cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca
    12481 aacgcttttt ggcaatcaaa gatgctttca gccatgatga caacgtacta gaccgctcca
    12541 tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag
    12601 aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc
    12661 cacaaaagaa accagatcta ctggtttata ttcgtgtatc ctttgaaact atgttagcga
    12721 gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct
    12781 actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca
    12841 aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa
    12901 tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga
    12961 tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa taatataaaa
    13021 actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt
    13081 tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg
    13141 tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa
    13201 acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta
    13261 gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt
    13321 tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga
    13381 ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg
    13441 cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata
    13501 gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg
    13561 ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc
    13621 attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa
    13681 aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa
    13741 gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat
    13801 atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt ttcagaaaac
    13861 taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat
    13921 agacaacgaa actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga
    13981 aggaaatcaa tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct
    14041 gggagaacaa gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta
    14101 cctatgaagc ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag
    14161 aaagtgaagg aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca
    14221 atcaagaaca aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag
    14281 gagctgggag cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa
    14341 aacaagtaga cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac
    14401 taaaaaaacg agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc
    14461 atagtatctt ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc
    14521 aaggaataca aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac
    14581 agctgatacg taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt
    14641 ctcggttaga ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta
    14701 aagaaaaata tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg
    14761 gattgctcac ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg
    14821 tactccaaaa tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa
    14881 aatatgatta tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg
    14941 tgaaacggat gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga
    15001 gtatctatgg attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc
    15061 ctaccaaaac tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag
    15121 caaatagatt gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac
    15181 aaaaagaagc aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt
    15241 ggctaatcaa acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca
    15301 tattataccg atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata
    15361 ttccttttgt actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc
    15421 aacatttcta tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc
    15481 aggcctacca gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat
    15541 gcgatcaata cttttctcct aaatatcaaa atatatccgt tgcagattat attttgtcga
    15601 taagccccaa tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta
    15661 aaacagacca gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta
    15721 tagctaagga aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg
    15781 ctactttcag aagactccaa gccttatcgc aagaaaccca agcgacagtt aaagaattaa
    15841 aaacccgctt gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc
    15901 acaaaagtaa aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt
    15961 tgccttataa cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac
    16021 cagtaacgac gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa
    16081 atcgtctgta tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat
    16141 ttatcaaaga aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca
    16201 gaagtggtca gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt
    16261 gaaatttcag cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga
    16321 ggtgtgacaa gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat
    16381 caaaatagag aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg
    16441 atttttttta tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg
    16501 gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt
    16561 cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata
    16621 tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag
    16681 gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta
    16741 acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac
    16801 gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt
    16861 catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata
    16921 acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac
    16981 tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt
    17041 gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg cagttttgaa
    17101 gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt
    17161 gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc
    17221 ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg
    17281 atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt
    17341 tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac
    17401 tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg
    17461 ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt
    17521 gttttaagta gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca
    17581 tgttagaaat gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg
    17641 attctatcga aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata
    17701 aatgagttgc tgtaagattt cgaaaaacga aatcaaaaaa acttttaaaa aagagttgac
    17761 aattacttgt ctctctgata tgatataaaa gttgctacgg cagccaaaaa cg
//
LOCUS       DACGLH010000055        17311 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.059, whole genome shotgun sequence.
ACCESSION   DACGLH010000055 DACGLH010000000
VERSION     DACGLH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.059"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            119..2653
                     /gene="secA"
                     /locus_tag="IXT21_001982"
     CDS             119..2653
                     /gene="secA"
                     /locus_tag="IXT21_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ4869367.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2779..3880
                     /gene="prfB"
                     /locus_tag="IXT21_001983"
     CDS             join(2779..2841,2843..3880)
                     /gene="prfB"
                     /locus_tag="IXT21_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ4869368.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            3985..4671
                     /gene="ftsE"
                     /locus_tag="IXT21_001984"
     CDS             3985..4671
                     /gene="ftsE"
                     /locus_tag="IXT21_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ4869369.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4655..5548
                     /locus_tag="IXT21_001985"
     CDS             4655..5548
                     /locus_tag="IXT21_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4869370.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5700..7148
                     /gene="cls"
                     /locus_tag="IXT21_001986"
     CDS             5700..7148
                     /gene="cls"
                     /locus_tag="IXT21_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ4869371.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7485..8342
                     /locus_tag="IXT21_001987"
     CDS             7485..8342
                     /locus_tag="IXT21_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ4869372.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8457..9377
                     /gene="pstC"
                     /locus_tag="IXT21_001988"
     CDS             8457..9377
                     /gene="pstC"
                     /locus_tag="IXT21_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ4869373.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9377..10261
                     /gene="pstA"
                     /locus_tag="IXT21_001989"
     CDS             9377..10261
                     /gene="pstA"
                     /locus_tag="IXT21_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ4869374.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10276..11082
                     /gene="pstB"
                     /locus_tag="IXT21_001990"
     CDS             10276..11082
                     /gene="pstB"
                     /locus_tag="IXT21_001990"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869375.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11098..11856
                     /locus_tag="IXT21_001991"
     CDS             11098..11856
                     /locus_tag="IXT21_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869376.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11869..12546
                     /gene="phoU"
                     /locus_tag="IXT21_001992"
     CDS             11869..12546
                     /gene="phoU"
                     /locus_tag="IXT21_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ4869377.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12784..13815
                     /locus_tag="IXT21_001993"
     CDS             12784..13815
                     /locus_tag="IXT21_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ4869378.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13812..14579
                     /locus_tag="IXT21_001994"
     CDS             13812..14579
                     /locus_tag="IXT21_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ4869379.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14679..16247
                     /gene="liaX"
                     /locus_tag="IXT21_001995"
     CDS             14679..16247
                     /gene="liaX"
                     /locus_tag="IXT21_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ4869380.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16547..16864
                     /locus_tag="IXT21_001996"
     CDS             16547..16864
                     /locus_tag="IXT21_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ4869381.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            16864..17214
                     /locus_tag="IXT21_001997"
     CDS             16864..17214
                     /locus_tag="IXT21_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ4869382.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         5681 a         2972 c         3768 g         4890 t
ORIGIN      
        1 gaagttctta ctttaatacg cttaagatat ttcaattatc tataactaat gataaaatga
       61 aaagtaaagt ctacatagtc tggataatac acagaaggaa aaggagatta cacaacgcat
      121 ggccaatttg attcgttcac ttatcgaaaa cgataagaaa gaactaaaac gtttagatag
      181 cattgcaaaa aaagttgaag catacgctga ccaaatggca gcattaactg atgaacaatt
      241 acaggcaaaa actgctgaat tcaagggcag ataccaaaaa ggagaaacat tagatcaatt
      301 acttccggaa gcttttgcag tcgtccgaga agcagcaaaa cgtgtattgg ggttatatcc
      361 acaccacgta caattgatgg gtggaatcgt gcttcacgat ggaaatatcc ctgaaatgcg
      421 tactggtgaa ggtaaaacct tgaccgcaac gatgccagta tatctaaatg ctctgactgg
      481 cgaaggggta cacgtagtta ccgtcaatga atatttagca acccgtgact cgacggaaat
      541 gggcgaactt tataatttct taggattaac agtggggttg aatatcaact caaaatcttc
      601 ggatgaaaaa cgcgaggctt ataattgtga tatcacttat agtacgaata atgaattagg
      661 atttgactac ttgagagaca atatggtcgt ttaccgccat caaatggtac aacgtccgct
      721 gaattatgcc attgtcgatg aagttgactc aatcttgatt gatgaagcga gaacgccgct
      781 gatcatttca gggcaagcag aaaaatccac agctttatat acaagagcag acaacttcgt
      841 gaaacgtttg aaagaagaag aagattataa aatcgatgta caatcaaaaa caatcagttt
      901 aaccgaagca gggatcgaaa aagcagaaga aacattcggt ttggataact tatacgacat
      961 tgaaaatact gcattgaccc atcacttgga tcaagcattg agagccaact atatcatgct
     1021 tttagatatc gattatgttg tccaagacgg aaaagtactg attgttgacc agttcactgg
     1081 tcggatcatg gatggacgcc gttattcaga tggacttcac caagcaatcg aagctaaaga
     1141 aggcgtagag atcgaagacg aaacgaaaac catggcgacg atcactttcc agaactattt
     1201 ccgtatgtac aagaaacttg cgggaatgac tggtacagct aaaacagaag aagaagaatt
     1261 cagagaaatt tataacatcc aagtatacca gattccgaca aatcgtccgg ttatccgtga
     1321 tgatcgtcca gatttgcttt acccaacatt acaaagcaag ttccacgctg tcgtgcaaga
     1381 tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc ggaactgttg cggttgaaac
     1441 atctgagtta ttatcagata tgctgaatca agaacgtatt cctcatgaag tgctgaatgc
     1501 gaaaaatcac tttaaagaag cagagatcat catgaatgct ggtcaaaaag gtgctgtgac
     1561 gattgctacc aacatggctg gtcggggaac agatatcaag ttaggacttg gggtaagaga
     1621 acttggtggt ctagcagtta tcgggacaga acgacatgaa tctcgtcgta tcgataacca
     1681 gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg tcacagttct acttgtcatt
     1741 ggaagatgat ttgatgaaac gattcggttc agaacgaatc aaatctttct tagatcggat
     1801 gaagatcggt gaggaagatg ctgttatcca aagcaagatg ctgacgaaac aagtagaatc
     1861 tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt aaaaacgtct tacaatacga
     1921 tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag cgacaagaag taatcatgga
     1981 agaaaaaagc ttatccaagc cactaatgaa catggtcaaa cggacaatca gtcgtgtagt
     2041 agatgcgcat acacaattag agaaagaaaa ttggaatttg gaaagtatca tcgattttgc
     2101 cggcagtact cttgtccatg aagatagtat tactcttgca gatatcgaag gaaaaacagc
     2161 tcaggagatc aaagagtatc taaacgaacg agcacaagaa gtatttaata ccaaagcggc
     2221 tcaactaaat ggtccagagc aactgttgga atttgaaaaa gtcgtgatct tgcgtgttgt
     2281 ggactctaaa tggacagacc atattgatgc aatggatcaa ttacgtcagt caatcggctt
     2341 acgtgcttat ggacagaaca atccattagt cgaatatcaa acagaaggct ataagatgtt
     2401 tgaagatatg gtaggcgcca tcgagtatga agtcactcga atctttatga aagctgagat
     2461 ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca gcttctcact cagaagacgg
     2521 agatgccaaa gaaaatagta atactagcgc gaaaaaacag ccaatccatg tgaacaaggt
     2581 cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa tataagaact gtcatggaaa
     2641 aaatgcaaac taatttctga cagcaggatt ggtgagacaa attctttttt taagaattcg
     2701 tctcaccttc tttctgttat ttgctgattc tgttagacta gatataatga aaaaagtata
     2761 gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa atctcctgac agaaatgcag
     2821 gaaaaggtca caagcttcag gaggtctctt tgacttagac cagctggaag aagacattgc
     2881 ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat gatagcgaag ctgctcaaaa
     2941 agtgatcagt gaaaacaatg caaataaaga aacttatgat caattccatt ctcttgctga
     3001 agaactggaa gaaatcgaat tgatggtgga gatgttgcag gaagaacctg atgcagccat
     3061 gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa aaaatgaaga catatgaatt
     3121 atctatgttg ttagatggtc cttatgatcg aaacaacgcc atcgttgaat tgcatccagg
     3181 ggctggcgga acggagtccc aagattgggg cagtatgctt ttacgtatgt atacacgctg
     3241 ggcagagcag catgggtatc aagtagagac actagattat caagcaggtg atgaagcagg
     3301 aatcaagagt gtgaccctac tgatcaaagg acacaacgca tttggttatc tgaaatcaga
     3361 aaaaggtgtt catcgccttg tacgtatctc gccttttgac tcagcgaaac gacgtcatac
     3421 ttctttttgt tcggtagatg tgatgccaga gcttgatgaa acgatcgata tagaaatcaa
     3481 tccagatgat ttgaaaatcg atacgtaccg agcaagtggt gcaggtggtc agcacatcaa
     3541 taaaacagaa tctgctgtac ggatcactca cttgcctaca ggaacagtag tagctagtca
     3601 ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg gggatgttaa aagctaagct
     3661 ttatcaatta gaagtggaaa agaaagaaca agaagcagct gcacttcgag gagaacaaaa
     3721 agaaatcggc tggggttcac agatccgttc ttatgttttc catccatatt ctatggtaaa
     3781 agaccatcgg acaaacgtag agacaggaaa tgtccaagca gttatggacg gtgaccttga
     3841 tttatttatt gacggctatt taaaaatgca tctacaataa gaacccattg ttaaattgaa
     3901 ataaaattgt aagattttgc aagcatgttg aaacattgcc atgctataat agctaaagac
     3961 tgaaaaagat atggagagaa cgccatgatt gaaatgaagg atgtaatgaa gaagtactca
     4021 aatggtacga ccgctatccg caatctctca attgtcatcg aacaaggaga gtttgtctat
     4081 gtggttggtc cgtctggagc cggtaaatct acattcatca aattgatgta tcgagaagaa
     4141 aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc taatagcaat caaaaaccgc
     4201 gaagttcctt atctacgcag agaaatcggc gtggtgttcc aagactataa attattacca
     4261 agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag ttatcggcag aaaacctcgg
     4321 gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg gtttgaaaca caaagttcga
     4381 gtctttccta gcgaattatc cggtggggaa cagcaacgtg tctctattgc tcgggcaata
     4441 gtgaatacgc ctaaagtact tatcgcggat gaaccgacag gaaatctgga tccagaaaac
     4501 tcatgggaaa tcatgaaatt attagatcgg atcaatgccc aaggaactac tgtcgtgatg
     4561 gctacacata acagtacgat cgtaaatacg attcggcacc gagtaattgc tatcgaaaac
     4621 ggccgcatta ttcgagatca agcggaaggg gaatatggat acgatgatta gaaccttttt
     4681 ccgccattta ttagaaagta tcaagagttt gaaacgtaac ggctggatga cagttgcttc
     4741 agtcagtgcg gtaacgatca cattgacttt agtcggtgta ttcatggcag ttatcatgaa
     4801 cgcaactaaa ttagctcaag atatagaagg aaacgttgat gtctctgtat tcgtagatat
     4861 cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa ttgaatgact tagatcatgt
     4921 taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa aaaatccaaa acgaaatggg
     4981 tgattcctgg aaattgtttg aaggtgacaa taacccgtta tatgatgtgt atgtagtaag
     5041 tgctactgat ccttcttata caaaacaagt agcagaagaa gcagcagatt tgaagaatgt
     5101 ttctcgttct gattacggtg gagcttcctc agatcggatt ttccaaatcg ctggtgcagt
     5161 tcggacatgg ggattagttg cagcggcatt gctgctgttc gtagcgatgt ttctcatttc
     5221 gaacacgatt cggatcacga ttttgtctag acagcgtgaa attcaaatca tgcgtttagt
     5281 cggtgcaaaa aatggatata ttcgctggcc gtttttcctt gaaggtggct ggatcggtct
     5341 gatcggtgcg attcttccga ttttgatcat cacatttggt tatgcatggt ttttccaatt
     5401 gatcaatcca tcattattac gttcccatta ttcattgatt catccacaaa atatcgtttg
     5461 gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt ggttcacttg gctcgatcat
     5521 ttctatgaga cgtttcttga aattctaata ccacaatata aataaaatga gaaagttgga
     5581 cagtttcgct ccagctttct ttttttattg gctaggagaa tagaaggaag cggctttttt
     5641 ccctttactt taaaaaacaa cttgggtatt cttaagttgg tagaagaaga ggtgaagccg
     5701 tgaaaattat cgttgacaat ttttttacga tccttttgat tatgaatatt ttgctttcct
     5761 ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac atgggcatgg gtgctcgttt
     5821 tgatgttcat tccagttgta ggatttattt tatatatttt tttaggaaga gggatttcca
     5881 aagagaaaat ctttgatttg aagatccaag ggaaaatcgg taagaacctg gaaattgaag
     5941 aagacaaaca ggcattgatg cgagggcttt atcctcatcc gcctactgga caagtagatg
     6001 tgaaacaatt gatctatatg ctcacggtat ttgaaagtac gctctatacg actaaaaatg
     6061 aaatcacttt atttacagat ggtcgtgaaa aattcgatgc gctgattgaa gatatcaagc
     6121 aggcagaaga ccatatccac ttccagtatt atatttatcg cagtgatgca ttaggtgaag
     6181 aagttcgaga tgcattgaca gaggcggcaa gacgaggggt aaaagtcaga gtccttcttg
     6241 atgcatgggg ttccacacaa gtttcttcca gcttttttca gaatttgaaa aaagcaggcg
     6301 gagaagtcgc atttttcttt cctctatttg ttccgtatat taatcctcgg atcaattatc
     6361 gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg atatacaggt gggttcaatg
     6421 ttggaaatga atatctagga ttggtaaaga aatttggtta ttggcgagac aatcatttgc
     6481 gtatttacgg agaagcggtg tataccttgc aaaaccgttt tttgatggac tggaattctc
     6541 agcatacaaa agaagtaggc tattctccca aattttttcc gtccattcac tctactggtg
     6601 atatagctgt tcaaatcgtg acaagcggac cagataccga acatgaacaa atcaaaatga
     6661 cttatttgaa aatgatttcg ttagccaaac gggagatatt gatccaaacg ccttattaca
     6721 taccggatgg atcgattcat gaagcactga agttggctct tctttcaggc gtaaaagttc
     6781 atatccaaat acctaacaaa ccagatcatc ttctcgttta ctgggcaacg tattctttcg
     6841 ctgcagagct aatcgagtat ggagcaagga tcgaaacata tgagaatgga tttattcatg
     6901 caaagacgat gattatcgat ggtggcattg tttcagttgg ctcagcaaat atcgatgttc
     6961 gttcatttcg cttggacttt gaggtcaata cactggttta tgatgaacga atggcttcac
     7021 gtgtaagaaa agcttttttt gaagactcga aaatttctac gcatctcaca aaagaaatgt
     7081 atgaaaaccg aggaataatc atcaagatga aagaaggatt agcaagattg atttcgccgc
     7141 tcctttaaaa cataaaaagg acatgagaac catcaacaga tggagatctc gtgtcctttt
     7201 ctaacggggc catttctgag agtacgtcag aaatggagaa agcgtctcgc atgagcgaaa
     7261 ttaactttgg ggtatctctt gttttttggg gaaagcaaga aataagctaa tatatgctat
     7321 taaaagagac acatttcaaa gccagtatag catagaaact tttaatgaga tagttttttt
     7381 tacaattatg agaatttatt ttgttattta atgattttga ttcgttttca tcaagtaatg
     7441 aaaatgataa aataaacata ctttaggatt aaaggagatt tctaatgaaa aaacttaaat
     7501 tattcttacc aataatagga ttagctattc ttttatcagg ctgttcaaag tggattgatc
     7561 gaggagaatc cattacggct gttggctctt ctgctttaca gcctttagta gaaacagttg
     7621 ctgaaactta ccagacagaa aatcctggaa aattcatcaa tgtacaaggg ggcggaagcg
     7681 gaactggttt gtctcaagta cagtcaggag cagtcgacat cggaaactct gatttgtttg
     7741 cggaagaaaa atcggggatc gatgcctcag cattagtcga tcatcgagta gcagttgttg
     7801 ggatcactcc tattgtgaac aaggatattg gcgtaaaaga catcagtatg gaaaacctga
     7861 aaaaactatt tacaggcgag atcaaaaact ggagtgaagt cggtggaaaa gatcagccag
     7921 tagttatatt gaaccgcgct tcaggaagcg gaacccgtag cacgtttgaa aaatgggtat
     7981 tggacggaga aacagctgtt caggcacaag aacaagattc aagcggaatg gtgcgccaga
     8041 tcgttgccga cacacccggt gcaatcagct atgttgcttt ttcttatgtt tccgatgatg
     8101 tagatacgtt gaatgttaac ggtgtagaac cgacagatga aaatgtgaca acgaacaaat
     8161 ggacgatttg ggcatatgag catatgtaca caagagatca tccaaaaaaa ctgacaaaag
     8221 aatttttaga ctatatctta tctgatgaag tacaagataa catcgttgga gaactaggtt
     8281 atatccctgt ttctcatatg aaagtcgaac gtgattggca aggaaatatc gttaaagagt
     8341 aatctttaca caaaatttac aaacttagct aaacttttac atgttattaa aaccaaattg
     8401 atgttcaatt tgtacactga gtgagtatgt taaacctgat taagggggag tcatctgtgg
     8461 aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc aaaaatggaa cagcgtggaa
     8521 aattcatcag ttttttatgt atcgcgctga tcgtgttggt tgtattatct attttttatt
     8581 ttgtagctag taaaggattg gccacctttt ttactgataa gatcaatgta tttgattttc
     8641 tgtttggtac agattggaat ccaagccaaa tcggagcaaa tgggaaacca atggtagggg
     8701 cattgcctat gatcacaggt tcgtttattg tgaccttttt atctgctatc gttgccacgc
     8761 catttgccat tggtgcagca gtattcatga cggagatctc tcctaaaaac ggaacgaaga
     8821 tcttacagcc ggtcatcgag ctgcttgtag gtattccttc tgtcgtttat ggttttatcg
     8881 gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg cgggactggg tttggtatct
     8941 tagctggtac atttgtgttg ttcgttatga tcttgccgac tgtcacaacg atgactgttg
     9001 atgcattaaa ggcagtgcca cggcactatc gggaagcttc attagcgctt ggtgccacaa
     9061 gatggcaaac aatctacaaa gtcgtgctac gagcagccgt gcccgggatt ttaacagcag
     9121 tcgtttttgg tatggctcgt gcgtttggtg aagccttagc tatccaaatg gtcatcggta
     9181 atgcagcatt gatgccaaca ggtcttacta ctcctgcttc tacattgacg tccatcttaa
     9241 caatgggaat cggaaataca atcatgggaa cagttgaaaa taatgtgtta tggtcattag
     9301 cgctgatctt gctattgatg tcattattct ttaatatcgt gatccgtatg attgggaaga
     9361 aaggagagct gaaataatga acgcaaaacg ctcagataaa attgctacag gtgttttata
     9421 tatagtttcg gggatcatcg tattgatttt agcagcttta ctactttata tcttagttcg
     9481 gggaataccg cacatttcat ggtccttctt gaccgaacct tccaaagcct accaagaagg
     9541 cggtggaatc gggatccagc tatttaactc gatttatttg cttttgatca cgatgatcat
     9601 cagttttcct atttctttag gtgcgggtat ctatctttca gaatatgctg gaaaaaactg
     9661 gctaacagat gtgattcgca cgtcgattga aatcttgagc tcgttgcctt ctgtcgttgt
     9721 cggtttattc ggtttcttgg ttttcgttat tcaattccag tatggtttct cgattatttc
     9781 tggtgcattg gctttgactg tattcaactt gcctcttttg actagaacag ttgaggagtc
     9841 tttacaagcg gtccattaca cacaacgaga agcaggattg gccttaggtt tgtcacgctg
     9901 ggaaacagtg ataaaagtgg tggttccaga agcgacacca gggattttga ctggtgtgat
     9961 cctaagttct ggaagaattt ttggtgaagc agctgcattg atctatacgg ctggacaaag
    10021 tgcacctgcg ttagatttta cgaactggaa tccattaagt gtttcaagcc cgatcagcat
    10081 tttccgtcaa gctgaaacat tagctgtcca tatttggaag atcaatacag aaggcacgat
    10141 gcctgatggt gtttctgttt cggctggcgc ttctgccgtg ttgattattg ctgttttact
    10201 gttcaacttc ggcgctcgtg cgattggcaa acgattatac cgaaaaatga cttcagccta
    10261 aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa caaatgtcat caccatgcca
    10321 aaagagaaag aagtagcact atatacggat gatctacatg tctggtatgg cgacaatgaa
    10381 gcaatcaaag gagtggatct ggaatttgaa aaaaataaga tcactgcact gattggtcct
    10441 tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc gaatgaatga tgggatcgct
    10501 aatacgaaaa tcactgggaa aatcatgtat caaggcgtag atattaatgc gcctcaagtg
    10561 gatgtttatg agatgcgcaa acggattggc atggtttttc aacgtcctaa tccgttcagc
    10621 aagtcgattt atgacaatat tacatttgct ttgaaacagc acggcgaaaa aaacaaaaag
    10681 aaattagatg aaatcgttga aacaagcttg aaacaagctg ccttgtggga ccaagtaaaa
    10741 gacagcttgg acaaaagtgc cttggcttta tctggcgggc aacagcaacg tttatgtatt
    10801 gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag acgagccggc aagtgctttg
    10861 gaccctattt cgacaggaac ggttgaagaa acacttgtaa acttgaaaga agactatacg
    10921 atcatcatcg tgacgcataa catgcaacaa gcagctcgta tcagcgacta tactgccttt
    10981 ttctatttag gaaaagtaat ggaatatgac aaaactaaca agatatttac cagaccaaaa
    11041 attcaggcaa ccgaagatta tgtgtccggt cattttggtt aagacggagg cctcaacatg
    11101 acaaaagaga ttatttcatc taaagatctt catctgtatt atggaaagaa agaagcacta
    11161 aaagggatcg atctaagctt caatcaaggg gaaatcactg cgatgatcgg tccttctggt
    11221 tgcggaaaat ccacttatct gcgttctttg aaccggatga atgacctgat tccgaatgta
    11281 acgatcaccg gaagtgtttt atataaagac aaagacatct atggtccaaa aacagatgtt
    11341 gttgaactaa gaaaagaaat cggcatggta ttccagcagc caaacccatt tcctttttct
    11401 atctatgaaa atgtcatcta tggattgaaa ttaaaagggg aaaaagataa aaaagtcctt
    11461 gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt gggatgatgt gaaagacaaa
    11521 ttgcataaaa gtgctttatc tttatccggt gggcagcagc agcgtgtatg tatcgcacgt
    11581 gttttggcag ttaatccgga aatcatctta ttggatgaac caacaagtgc gcttgatcct
    11641 gtttctactg ggaaaatcga aagtatgctg ttagaattaa gagagcggta tacgatgatc
    11701 atcgtgaccc ataatatgtc tcaagcttca cgtatttcag ataagacggc attcttttta
    11761 gatggtcact tgatcgaata caatgatacg aagaagatat tcatgaatcc agaaaaacaa
    11821 gagacagaag attatatttc aggtcgattt ggataaggag gaaacggaat gctacgtaca
    11881 caatttgaag aagaattgct gaatctccat aatcagttct acgaaatggg gatgatggtg
    11941 agcagcgccg tgcacaagtc agttcgtgca tacatcaaac acgataaaat cttagcccaa
    12001 gaagttatcg aaaacgatat aaatatcaat aacatggaaa cacgcttgga gaagaaaagt
    12061 tttgaaatga tcgcattgca acagccagtt acaactgatt tgcgtatgat cattacggtc
    12121 atgaaagcta gctcggattt agaacgtatg gccgatcatg cggtgtctgt tgctaaatcc
    12181 acgattcgcg taaaagggca gacgagaata ccggaaatcg aaaaagagat ctccgatatg
    12241 tccgattatg tgaaaaaaat ggtggacaac gtcctagttg cttatgtcaa aacagacgaa
    12301 aaagacgcac gtacgatcgc caatatggat caaagagtca atgactattt caatcgaatc
    12361 tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga cagtcatcag tggaacagac
    12421 tacttgaatg ttgccagtta tctagaacgg atcggagact atgtgacaaa catctgtgaa
    12481 tggatcgtct acttggctac agggaaaatc actgagttaa atacgaacca caatgagatt
    12541 ttctaaactg aagctgtgat tatgccgttt gtttaggaaa agaggttgtg aaatcagcgg
    12601 ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc aaacaaaaaa tagctcctca
    12661 tatttttcgt ttggctggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg
    12721 ggaaagggtt tgtgacaaaa catgtcacaa acccttttca tcatttcata gaaaggataa
    12781 agaatgcaaa aaaaaattag catcatcact atatttttta tttgtgtgct tacttcatgg
    12841 gttattcagc atcaagccgc tttatatgaa gtacctgtca tcgagataga aaaagtacga
    12901 ataaaagagg atcatcaatt gattacagga aaactgatga acacggatca aaaaggagaa
    12961 tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg
    13021 agacaaatgt atctagtcca aatgcatgga gatgaatggc agctagtagc aaaaaaaaat
    13081 gatggctggc ttttcttctt cacaagtatc ttcattggcg ccatgttgct tattggtgga
    13141 aaacagggtc tatttgcttt aggaagtctc atcatcaata tcgttttact tttacttttg
    13201 ttgctcatct ttactaaaac agaagggatt tctctattaa atatatctat cctgttcgtc
    13261 gttataggga tgattattag catattggct ttagatggat ggaatagaag cagtataatc
    13321 aaaatttcag ctgcagttac gagtgttttt ttagcatttt ttatttgtct tttgacaatg
    13381 aatcattgga aagaccaagg actgcgctat gaggaactga attatctgac acgaccgtat
    13441 cgttctgttt ttctttctag cgtactgatt ggaacagctg gaggaacact cgataccgtt
    13501 atcacagtca ttgcgacatt agaagaattg actaagcgac aaccagaaat aacagcgaaa
    13561 cagttatggc tttcaggaag gcaagtgggt cgagatgtga ttggctcgat gaccaatatc
    13621 cttttgttcg tctatatcag tgggtcagtt ccatcgcttc ttttatattt aggaaatcaa
    13681 tggtcgttca aagaaacgat agaacagcat ttatcattgg aatttttacg cgtcattgct
    13741 ggcagcttag gaatagtatt atcgatccct atctcggtcc tcttttttct gactgtaagg
    13801 aggctgaaac gatgaatgta cttattatac ttgttgggat tttagcaatc agcgtgctac
    13861 ttattggcgg tacgcaaggt atgtatattc ttttaggctt attcatcaat ttagggatct
    13921 ttttcctgtt gttattcggt tatcatcaga agtggccgat actggcattg tcggtcatag
    13981 gatttttatt gattgctgtg gttatcttat tttttatcaa cggttataat ctaaaaatgc
    14041 gtgcagcttt tgtgagtata ctgatctttt tattttgttt cctactattg cttcctatca
    14101 ctgatttttt agcaatccaa ggttttacat cggtagaact agaagaatta tctggcttag
    14161 ataagacttt agcgattgat tttagattac ttacgagatc gttgcttctg attagtctta
    14221 gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg gacattcgaa gtatatcagg
    14281 cgaatccgca attatctttt taccaattgc gccattctag tttcgctatt gctaaaaaaa
    14341 tattggcgtc aacagtaatg acacttcttt ttgcattcat gggaagttct ttagcgttga
    14401 tcctctggtt tattgattta gacattcctt ttcagcaagt gatcaatgaa aaatcatttg
    14461 ttctagaata tacgatggct attcttacca cattgtccgc actacttgtt cttcctttaa
    14521 cctgcgtgac tacagcatat ctctttacaa aaaagaaaga acaactcaag atggagtaaa
    14581 agttacaact taagtcctat gacattttta ctgaatcagt gcataatatg gatgtaagga
    14641 aaagcttgca aacaattttg cttaaggagg caatttccat gaaagaaaga gaacgtattt
    14701 tagatttagt gaaaaaagga atcctttcga ctgaagaagg cttagattta ttagaaagca
    14761 tggcaactga aaaagacgaa aagcaaatca aaaaagaagc agacaaggta actgctagtc
    14821 ataaagaaaa agatcaggca agtcaattga tcgataaact tgaaaacggc gaagaagaaa
    14881 tttctgagcc agttgatcca aaggaaagag aaagacaaga tcaagaaaac ttagaaaaga
    14941 tcttagatga actagcaact gaagcgaata aaacttcggc gcgtctagat gaagtaaatg
    15001 ctcaattcgc tgacaataag gcagcacgca atgagaaaca ggaagcattg atgcagctga
    15061 atacaaaaga agaattaggc gagttgacag aagaagaatt agcacagcgt cagacgttag
    15121 aagcagaaat caaagaatta gaagctagtg gcgatacact gttagaagaa caattgaaat
    15181 tagaagcaga attaaaagat atccgtaaga atcaatggtc tgagaaaaaa gaaacgttta
    15241 ctgataagtt tgaattgcca gatgattgga aagatcaagc aacagataca ctgaatcaag
    15301 taggcgaaaa aatgtcagaa gctggatcac agttaggcaa attcttgaag aaaacattcc
    15361 aaactgtttc agaaacagtg aatgacaaca tggaatggaa agatgtcagc ttacgtgtcc
    15421 ctgggattgc gacaacgaaa tttgagcatg aattttacta tgaagctcct gcagcaagta
    15481 tccttgatat caaggcagca aacggaaacg tgacgttaaa aacgtgggac agcaacgatg
    15541 tgaaagttga agctaagatc aaactttacg gaaaaatggg tgctgaacca tttgaagcat
    15601 tctctgaacg gagccagatt gaggttaatg aagatcatat ctccttccaa attccaaata
    15661 aacgtgtgcg tgcagacctt gttttctact tacctaaacg agtatacgac cacgcagcta
    15721 ttaaacttct aaatggcaac atcatgattg aaacattgga agctaaagat atttatacaa
    15781 aatctacgaa tggtaacatc atcgtggacc aattaactgc aacgatgctg gaagtagaag
    15841 gtgtaaacgg caatatcgat attcgaaacg ggaacatctt agattctatt atcgaaacag
    15901 taaatggtac agtgacattt ggtgcgacag cagaaaacct ttctgtgtct ttggtaaatg
    15961 gtgatgtacg tcttacgatt aaagaagata atttgaaaaa agtagaagca agttcagtga
    16021 acggaaatgt caaagtcgct ttaccagatg gcatcggttt ggaaggacat gctaaaacaa
    16081 gtcttggaag tatcaacagc cgtctgtcca attacgaagt agttcgtgag aaaaaagaac
    16141 ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg tgaaatcgcg caagtgcaac
    16201 tttcaacgac aacaggaagt atctatttaa aagatacaga taaataaaga ggttgtgaaa
    16261 gagatgttca gccttgagca ataagaagcg attttgtaaa atagcttccc ctattttaca
    16321 aaattgtgcc ttattgccga tagtctatct cttgaacacc gtttaccaag agattatgaa
    16381 cctgccaatt atcgccgagt cacaaggaat aatttcagaa aaagctcgtc acgttttatg
    16441 aatatggtaa tttgtgaccg agaattttgc agaaagacac cgattaagaa gaaggttgtc
    16501 acaacgattt tcttcattta aaatagtaga acagaggtga gacatgatga aaaaaaagtt
    16561 gacaaaatct aataaaaatg ttgttttgac agggacttta gcaggaatag gagaatacat
    16621 cgggattgat ccgactgtta tccgtgtgct ttatgtattc ctgagcttag ttgcttttgg
    16681 aagtccaatt attttataca ttttattagc tttgattatc ccatctgcgc gttctgcacg
    16741 aacaaactat ggacataaca atgcatatta tgaaaaaaac aattatcgtg aacaaactcg
    16801 tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat ggagtgactt
    16861 ttaatgtcgt actttcaacg tattttagtt aatacgttaa cattcatatc attggctgtt
    16921 ctttttcctg cgatggtcca tgtgagcagt atttggacgg ccgttcttgc cagcttcgta
    16981 ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga tactgtccct gccctttaca
    17041 ttagtcacat tgggattgtt tacgtttgtt atcaatgcag cgattcttaa aatgacctcc
    17101 ttttttgtgg gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt gttagtagct
    17161 atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata acagtagaaa ataaaagaaa
    17221 gctgtgccga actatcggcg cagttttttt atttagaatc aggaatgcgt tttctattac
    17281 attggtaatt aaataaaaac gtttttttta a
//
LOCUS       DACGLH010000056        17224 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.060, whole genome shotgun sequence.
ACCESSION   DACGLH010000056 DACGLH010000000
VERSION     DACGLH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.060"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            1..369
                     /locus_tag="IXT21_001998"
     CDS             1..369
                     /locus_tag="IXT21_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ4869383.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="IXT21_001999"
     CDS             549..1490
                     /locus_tag="IXT21_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ4869384.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="IXT21_002000"
     CDS             1559..2290
                     /locus_tag="IXT21_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ4869385.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="IXT21_002001"
     CDS             2434..4614
                     /locus_tag="IXT21_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ4869386.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="IXT21_002002"
     CDS             4634..5116
                     /locus_tag="IXT21_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ4869387.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="IXT21_002003"
     CDS             5132..5893
                     /locus_tag="IXT21_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ4869388.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="IXT21_002004"
     CDS             6024..7358
                     /locus_tag="IXT21_002004"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ4869389.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="IXT21_002005"
     CDS             7665..8150
                     /locus_tag="IXT21_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ4869390.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="IXT21_002006"
     CDS             8143..8826
                     /locus_tag="IXT21_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ4869391.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="IXT21_002007"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="IXT21_002007"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ4869392.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="IXT21_002008"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="IXT21_002008"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ4869393.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="IXT21_002009"
     CDS             12442..13071
                     /locus_tag="IXT21_002009"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ4869394.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="IXT21_002010"
     CDS             13123..13455
                     /locus_tag="IXT21_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ4869395.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="IXT21_002011"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="IXT21_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ4869396.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="IXT21_002012"
     CDS             14281..15654
                     /locus_tag="IXT21_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ4869397.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="IXT21_002013"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="IXT21_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ4869398.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="IXT21_002014"
     CDS             16709..17149
                     /locus_tag="IXT21_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ4869399.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5874 a         3008 c         3795 g         4547 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggg
//
LOCUS       DACGLH010000057        17005 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.061, whole genome shotgun sequence.
ACCESSION   DACGLH010000057 DACGLH010000000
VERSION     DACGLH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.061"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..124)
                     /locus_tag="IXT21_002015"
     CDS             complement(<1..124)
                     /locus_tag="IXT21_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ4869400.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="IXT21_002016"
     CDS             complement(228..539)
                     /locus_tag="IXT21_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4869401.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="IXT21_002017"
     CDS             complement(541..738)
                     /locus_tag="IXT21_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ4869402.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="IXT21_002018"
     CDS             complement(1132..2859)
                     /locus_tag="IXT21_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4869403.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="IXT21_002019"
     CDS             complement(2852..4045)
                     /locus_tag="IXT21_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4869404.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="IXT21_002020"
     CDS             complement(4232..4876)
                     /locus_tag="IXT21_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4869405.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="IXT21_002021"
     CDS             complement(4970..5104)
                     /locus_tag="IXT21_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ4869406.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IXT21_002022"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IXT21_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ4869407.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="IXT21_002023"
     CDS             complement(7235..7708)
                     /locus_tag="IXT21_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ4869408.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="IXT21_002024"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="IXT21_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ4869409.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="IXT21_002025"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="IXT21_002025"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ4869410.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="IXT21_002026"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="IXT21_002026"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ4869411.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="IXT21_002027"
     CDS             complement(11755..12894)
                     /locus_tag="IXT21_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4869412.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVFQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="IXT21_002028"
     CDS             complement(13203..13916)
                     /locus_tag="IXT21_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ4869413.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IXT21_002029"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IXT21_002029"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ4869414.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="IXT21_002030"
     CDS             15105..16637
                     /locus_tag="IXT21_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4869415.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="IXT21_002031"
     CDS             complement(16828..>17005)
                     /locus_tag="IXT21_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4869416.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3495 c         2909 g         5322 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg aacactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DACGLH010000058        16904 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.062, whole genome shotgun sequence.
ACCESSION   DACGLH010000058 DACGLH010000000
VERSION     DACGLH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.062"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..190)
                     /locus_tag="IXT21_002032"
     CDS             complement(<1..190)
                     /locus_tag="IXT21_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869417.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="IXT21_002033"
     CDS             complement(200..1015)
                     /locus_tag="IXT21_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4869418.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="IXT21_002034"
     CDS             complement(1277..1720)
                     /locus_tag="IXT21_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ4869419.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="IXT21_002035"
     CDS             complement(1854..2192)
                     /locus_tag="IXT21_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4869420.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="IXT21_002036"
     CDS             complement(2180..2557)
                     /locus_tag="IXT21_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4869421.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="IXT21_002037"
     CDS             2781..3974
                     /locus_tag="IXT21_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4869422.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="IXT21_002038"
     CDS             complement(4140..4562)
                     /locus_tag="IXT21_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4869423.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="IXT21_002039"
     CDS             4957..5151
                     /locus_tag="IXT21_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869424.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5642
                     /locus_tag="IXT21_002040"
                     /pseudo
     CDS             5148..5642
                     /locus_tag="IXT21_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5804..6976)
                     /locus_tag="IXT21_002041"
     CDS             complement(5804..6976)
                     /locus_tag="IXT21_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4869425.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="IXT21_002042"
     CDS             complement(7182..7997)
                     /locus_tag="IXT21_002042"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4869426.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="IXT21_002043"
     CDS             complement(8147..8593)
                     /locus_tag="IXT21_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ4869427.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9594)
                     /locus_tag="IXT21_002044"
     CDS             complement(8590..9594)
                     /locus_tag="IXT21_002044"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4869428.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9634..9963)
                     /locus_tag="IXT21_002045"
     CDS             complement(9634..9963)
                     /locus_tag="IXT21_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ4869429.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10089..12416)
                     /locus_tag="IXT21_002046"
     CDS             complement(10089..12416)
                     /locus_tag="IXT21_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4869430.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(13259..13552)
                     /locus_tag="IXT21_002047"
     CDS             complement(13259..13552)
                     /locus_tag="IXT21_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ4869431.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(13955..15133)
                     /locus_tag="IXT21_002048"
     CDS             complement(13955..15133)
                     /locus_tag="IXT21_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4869432.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(15237..15551)
                     /locus_tag="IXT21_002049"
     CDS             complement(15237..15551)
                     /locus_tag="IXT21_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4869433.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            15658..15873
                     /locus_tag="IXT21_002050"
     CDS             15658..15873
                     /locus_tag="IXT21_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869434.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(16173..16415)
                     /locus_tag="IXT21_002051"
     CDS             complement(16173..16415)
                     /locus_tag="IXT21_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4869435.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(16402..16830)
                     /locus_tag="IXT21_002052"
     CDS             complement(16402..16830)
                     /locus_tag="IXT21_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4869436.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         4927 a         3271 c         2935 g         5771 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca taacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag cgaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aaaaatcatc aaataatcct ttacacgtta ttctaaattt aatttttatt
    12481 attttattga tgtatagaaa gtttggggct ttttttctct ttttattcta aggttatttt
    12541 agtcttgtcc tttaatacaa agattttatt tttaaataaa aaatactctt tttttacgat
    12601 tattgaaaaa aaaagataaa acttgattaa ttgaagtatt gcttaaattt ggaagctatg
    12661 agggaagaat aaaagagtta ggacgggatt atctactatt tttctttgtt ttaaatattt
    12721 gttatctttg aacataaaag agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa
    12781 taaacggtgt tcaattagct gatcttgtat tcacttaaaa aatggtaaag tacgagttgc
    12841 tattccccat tttcaatttt ctaacgctca ggacaaaata ctgttttcac aacctcattg
    12901 gaataaaagc atatatattg tttcaagaac gaaatcttgt tatcttttta aagggcatct
    12961 gtccaaaatc taattttagg aggtacgttt tggtatttat tcgtacaaat aaaagagaat
    13021 tatctttatt catgtaacgc atccaattga ttttttcatg tacgctcctg gaacgatttt
    13081 actgacgatt gcttatttaa ctgcgaatcg ctcattagct tttatcatag ctatccatat
    13141 tttgaataac gttcttggct ttgtattata gatacagatg acaaaaaaag ttcaagtatg
    13201 cggtgtagaa ccaatatcgc atacttgaac ttttttcaat gagtataaat taaaacttct
    13261 aattttcaat tcccatcatc tgatatgccc gaccgtctga acttcttttg aaaaagccaa
    13321 atgtgattag ataacgtctt tgttcgacat aatcgccact agaaaatacg ttcagcagta
    13381 tcgaattaac ctctgtttct gtataagtca ctccatggtc aaagcgtttc aagacttctt
    13441 tcaataggat gtatttcttt tttgttgaac ttggtacttg ttttactttt ccatcattca
    13501 tgaatgtgtc taaaactttc atcttttctt gctccgtata agtataaacc atcttctttc
    13561 tccttttgat tctaagaata ctccatttat gttagaatat tctggatttt ttattaaagc
    13621 ggttactctt aagaaagata ttatcatagt gctctttttt ttactactac ttataggtag
    13681 atttattcta atccattatt ctcccctacc aataattaaa gaaaaaactg gatcagggtg
    13741 ttcaaattac atgctaagaa aaaagaaccc ttgaatacca agggttcttt ctcagtctga
    13801 taacaatgat taacgacgga tttctttgat acttgtgaaa agtcgttaaa gtattgatat
    13861 aaaaggattt ttattacctc tgactacata ttgactaatt tgttaaaaaa tcgtttttca
    13921 agaatcctgt aataccagtg ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa
    13981 tcagaggttt taccttttca tttgacgtta agtggcgata aattttcgaa gttgtttggt
    14041 cagtatggcc taagcgatct ttcaagactt cccagctaca gtgttccgct actaagcttg
    14101 ccattgtatg acgagctaaa tgagtatgaa atggcttatt aattcctgag cgattcacgc
    14161 accgtttgac gtgatcattt acatactctc gacgataagg aacaccacgt ttactagaat
    14221 caagatacac aaacaaatat gtttcttcta taataaacct gtaatgttgc ctcatctcac
    14281 ccaaagcctt aatcaactgt tgtagctttt ccataactag aggagttact agaactcgtc
    14341 tagcagaact aactgtctta gtagtttcaa ttcgaaaatc cttactctta cctttaactg
    14401 ccgtttgctt atagaacaga atttcatttc ttttgaaatc tatatctcca ggctgtagag
    14461 caagcgcctc gctgactcgg cagccagtgt aaaatagaac atggaaaagt acagacatcg
    14521 gtaattcctc atgaacaatg ccataattca aaaaagttct caattcatcg acagtaagaa
    14581 acttgtcctc aatattattt atggcttctt ttaaatcagt taccgttaac tgatattttg
    14641 gcattctcac agacttcatt ggattttcac ttatcaaatt ctttctttga caatatttaa
    14701 aaatcatatt tagaacttga ataagtgatt gtaaactatt tttggcatag ccaacatttg
    14761 ttccattaac gccacattcc tttaaggtaa aaagcaattc ttgaaggcac tctggtgtca
    14821 aatcagacaa ctttttatca ccaatccatc gaacaataat ttttaattgt tcctctcgct
    14881 tatcataggt tatttccttt tgttcattta acgaacggta atattggaac cattgctcac
    14941 ctacaacact aaacttgtca ttgcttgcaa caattttttt cttaagcctt tgttgtttct
    15001 ccatttccag ataagcgagt tgcgcctcac gttttgaatt aaagccacgt cttgttgttc
    15061 gatatgcttg aaagtaccat gtgatcctac cagtagaact gatttgcttc ttgaatcgat
    15121 ctgttttttc catttattga ttcctcctat tttgaaggaa cccaacaagc agaaaccctt
    15181 tggaaatctt tctatctatg tcatcattgt accgcatctt agaaagaaaa tgtaacttat
    15241 aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg aatcactctt tctcatccat
    15301 tctaaaacat catcacgaaa atagtgtcct cgaatcccgt accaacaggg aggaaactca
    15361 ccaagtttta ctaaacggtt cagtgtacta tcacttatct gaaatagtcg ctttaaatca
    15421 gtatttgtta ataccagagg taaatgactg actgcaagca atttttccgt gtaatcttcc
    15481 agtttttttt gtaaaattga atagaactct atttccagag aattaacatt tgttttaact
    15541 tccaactcca tcacccttct ctctcaaaat caaaacgacc accacggtac cccttaaaat
    15601 tgtaaaaaaa cactccagta ctccatcaaa acggactaga aaagaggaat ttttaatatg
    15661 aaacaaggaa ttgcagacat aaaaataatt aaagaaattc ttgaaaaatc tacggctaat
    15721 gcaatagcat tcggaacagg aatcaacttg agtacagtaa aaaaacttaa aagtggagag
    15781 agagctgaag aaaaattaaa tcttgccgat gctattaaaa tcacagaatt tggcatgaaa
    15841 aatatgccaa ccaaaatcga aatatggaaa tagtagtttt tctgatctat cagctgtttt
    15901 gccaaagcac ataggaatct cacctccccc cagtaatggc gggccgattt ggttacggaa
    15961 gtatcattat cgtagtcgcg attctcatat taaaatttgt cgcttaaaag ctaaccttgt
    16021 catggcgtaa ctagagaatc aaaatctaca tgtactgata gatttgctat tcatttgtca
    16081 aagagcgcta ggctattttc agcctatgga aatcctttat tttgacccga taaaaaactt
    16141 ccatagaatg aaaaggaaag ggaaaaaata ctttaacgag ttgtatcaaa ttcaagtatc
    16201 ttctcaatca attttgtttc cagttgtcta cgcatatatt catctacata cataaccatt
    16261 cctttttctt cttcataaac tggtttcatg gctaaccgtg aaatataatt ttgatatttt
    16321 gacagaacga tggacatggc aattgaatca ccagaagctg cggctttgat tgtcgttagt
    16381 ggtatgaatg gtatatatcg tttattttgc ttcatatttg acacccaaat actttcctag
    16441 ctcttccaaa gccatttgtc gttggtacca aacactgccg agacttttcc caataatatc
    16501 gccaatttca cgatcattaa gaccgatgaa gaaggacata aaaatgattt cttgtctttg
    16561 actaggtaat gctgcaatcg catttgccaa ctcctcattg gaaataaaga ttttcatacc
    16621 aaggacaata aaagtaccac tactattcaa atttggttcc aaataataga actctgtacc
    16681 taaccctttt tgttccaata tttccattga tatttgccgt tggcgaaatc tagcgtactt
    16741 cttttgaata tttaacgcct cattgcagac tactttctta caaaaactgt caaacgtatg
    16801 ttgaatgtgg gtttcctctg agtcgcccat aatattctcc ccctcaaaaa attcattcaa
    16861 ctcgttttac ttctatccct ttccattatt actacaatct agga
//
LOCUS       DACGLH010000059        16710 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.063, whole genome shotgun sequence.
ACCESSION   DACGLH010000059 DACGLH010000000
VERSION     DACGLH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.063"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(41..1147)
                     /locus_tag="IXT21_002053"
     CDS             complement(41..1147)
                     /locus_tag="IXT21_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ4869437.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1357..2358
                     /gene="ccpA"
                     /locus_tag="IXT21_002054"
     CDS             1357..2358
                     /gene="ccpA"
                     /locus_tag="IXT21_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ4869438.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2695..5040)
                     /locus_tag="IXT21_002055"
     CDS             complement(2695..5040)
                     /locus_tag="IXT21_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ4869439.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5319..6221
                     /locus_tag="IXT21_002056"
     CDS             5319..6221
                     /locus_tag="IXT21_002056"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ4869440.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            6434..8209
                     /locus_tag="IXT21_002057"
     CDS             6434..8209
                     /locus_tag="IXT21_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ4869441.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8275..9861)
                     /locus_tag="IXT21_002058"
     CDS             complement(8275..9861)
                     /locus_tag="IXT21_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ4869442.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            10112..10336
                     /locus_tag="IXT21_002059"
     CDS             10112..10336
                     /locus_tag="IXT21_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ4869443.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10478..12229
                     /locus_tag="IXT21_002060"
     CDS             10478..12229
                     /locus_tag="IXT21_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4869444.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12229..14013
                     /locus_tag="IXT21_002061"
     CDS             12229..14013
                     /locus_tag="IXT21_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869445.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14339..15190
                     /locus_tag="IXT21_002062"
     CDS             14339..15190
                     /locus_tag="IXT21_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4869446.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15283..>16710
                     /locus_tag="IXT21_002063"
     CDS             15283..>16710
                     /locus_tag="IXT21_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4869447.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFTLTFFFWKDEAGETDDIQKK"
BASE COUNT         5168 a         3058 c         3429 g         5055 t
ORIGIN      
        1 taaaaaggtg ggagcggact agctctcacc ttttttactc ttatttttct aaaacaagta
       61 attcttttgg tgtagtcgtg aatggcacac agccatcgct tgtcacatat acacagtctt
      121 cgatacgtac ccctactttt tcaggaatat agattcctgg ttcaagagag aagcacatcc
      181 cttcttcgat tactaagtca tttccttcta caagggaagg gaattcgtgg acggttgttc
      241 cgatcccgtg acctaaacgg tgattgaaat attcgccgta gccatagctg ttgatcacgt
      301 ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg acggacggct tcttgtgcgg
      361 caagctgtgc ttcaagagtg atgttgtaga ttttttcttg gaagtcggtt ggtttttgat
      421 aagcaattgt acgagtagca tcactgcaat agccattcca aacaacgcca agatcgaata
      481 acacaagttc attcggtgaa atcttcgtat ttcctggtgt accatgaggg cttgcagcat
      541 ttgctcctgc aaggactaat gtgtcgaaag acatttgtga aacaccttgt tttttcaatt
      601 ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc ttctttcact gctttaaagc
      661 caatctcaaa tgcaacatcg gcccatttac cagcttcgat cagtttgtct ttttcctcta
      721 gagttttgat cagttgtaga cgttggatga tggaagttag atcagtgtaa gaatccgttc
      781 ccgtcaagat atcagagata gcatcgaagc gctcgacagt caaggcgttc ttttcaatcc
      841 ctacattgcc aggctgacca tagcgagcac gaagttcttt tccgatgatc tcccaagggt
      901 tttcgctgtc taaatagcca cggacatcat acgcccaact actgttttga gcatcttcaa
      961 cttccaaagc aggagtgaaa aggaaaggat cttgttcaag agggataaaa agagctagta
     1021 cacgctcatg aggatcactt tcgtatccgg aaaagtaagc gatgtggcca gggtcgctga
     1081 tataagccaa ttcgacttgg tgttgtgcca tccattgacg tagttcattg atttttacgt
     1141 tattcatttg gcaacctact ttctaaatta attttgtatt caataattgt accactgatt
     1201 tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa attttcatga aaacagagat
     1261 aaaacggttg caattcttga gattatttga tattctattt tagaaatgta agacgtattt
     1321 tcagaaaaca tataaaatat gaataggaga acacaaatgg aaaagcaaac aatcactatc
     1381 tatgatgtag cgagagaggc aaatgtttcg atggctactg tttcacgtgt cgtaaatggc
     1441 aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg aagttatcga tcgcttggat
     1501 tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga aaacaacaac agttggtgta
     1561 atcatccctg atgtcagcaa tatgttcttt gcttctttag cacgtggtat cgatgatgtg
     1621 gcaacaatgt acaaatacaa tattatctta gcaaattcag atggcaacga ccaaaaagaa
     1681 gtcaatgtat tgaataactt actggctaag caagtggacg gcgtgatctt catgggtcac
     1741 catatcacag acgaaatccg tggagaattt tcacgttcaa aaactcctgt tgtattagct
     1801 ggctctattg atccagatga acaagtaggt agcgtaaaca ttgattatgt atccgcaaca
     1861 aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa aaatcgcttt tgtcagtggt
     1921 gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag gctataaaga agcattaagc
     1981 gaaaataacc taccatatag cgaaggattg atctttgaag cacaatacaa cttcaaagat
     2041 ggtctatctc tagtcgatcg gatccataat agcggtgcaa cagctgtcta tgtttcagat
     2101 gatgaattag ccatcggtat tttggatggt ttattagacc gtggcgtgaa agtaccggaa
     2161 gaattcgaaa tcatcacaag taacaattca ctactgacag aagtagctcg tcctcgttta
     2221 acaagtatca cacaaccatt atacgattta ggggcggtat ctatgcgcct attgacgaaa
     2281 atgatgaata aagaagaagt cgaagaaaaa acgattatct tgccttatgg aatcgaagaa
     2341 aaaggatcta caaaataata aaaggacaac cattcagagg ttgtgtgcct gccgtttaac
     2401 tctgcgttac aaggagcaat ttcaaaaaac agcatctcat gtttgatgaa tattgtgact
     2461 cgtgattcgg agttggcagg agaacaccgt ttatttggga aaaggggtta tgacaaaatt
     2521 ttgttataat cctttttttg tgtcttaaaa aagagagaaa gtggttgaaa ttacatttat
     2581 cgtatataaa acaatccgtg tatagatgct gcattttgtt caccaccatc aaaaaaataa
     2641 gggactgtga caatactatt gtcacaatcc cttattactg tgtattctat taaatcaatc
     2701 atctttctca tctgattctt tagttgcttg attcgctctt gcataagctg aagcggtgtt
     2761 ccagtaatct gtatagtttt catcggtacc gccaacgcca aatttaaagg cgcttttctt
     2821 cggtccattt ttcgcccata gcgattcaac tgtttttcct ggtgtttgga tggatttttt
     2881 attgacagta acttttccag gtgtttgacc agtaaatcca gatacttttt tcttcatcac
     2941 atcagatgat aattcgaatt tctcatcagt gccaaaaatg tcaggttttg cttggtaagc
     3001 ttgattgatc agattggcaa gataattagc cgaacgtatc cttgctttgc tgtccattcc
     3061 agtattgtca tcgtgccctg cccagctact gatggtaata gacggagtag agacaattag
     3121 ccatgaatct cggtattcat tggtagaacc agtttttccg acccaatctg cttttcctag
     3181 atttccgttt aagcttgaga tggcatcttt gaatggtgta gtgatttttg cattgatcac
     3241 actacgcatc atgtcattca tgatcgatgc cgttgcttcc gaatagatcc ggacagagtt
     3301 gctttcgtgt ttataaatta catttcctgc gttatctgtg atggaatcga tgatatagcc
     3361 ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg ttggtctgtt gaagcgtcgt
     3421 gacatctact gtacctaaag gagcagattc aacgccccaa ttgtcatttg ccgggtagtt
     3481 catctttgcc aagtagtgct cgtaagcata ttgtttcgat ccaccggaat tcaacacatc
     3541 ttgatataag tgataagccg ggatattgtt ggaccattct agtgactcac ggacggtttg
     3601 gaaggtgttc gatccttcgt tagtagcatt gacgattttc tcgccagcat tttctccttc
     3661 ttgccaagtt gttgcataat ctgatacacg ggattctgat ccgataagcc ccatgtcgat
     3721 tgcaggtcca taaacaagaa ctggtttgat tgatgagcca gcttgacgtt ctgtatcgaa
     3781 ggcatggtta ttttgattct gactgtaatt ccgtccgcca acgaatccgt agatgcggcc
     3841 agttctgtta tccatcaata catttccggt ttctacttga ctgccagcac catcatctaa
     3901 catgtatcca taattttcaa cgccattttg caatgcagca tagacatctt tatcgattgt
     3961 cgaatggatt gtatatcctt tattttggat cgtttgctgt gccttttgat aataagcgtc
     4021 actgaccgat gaatcagaca tatcagcgtt gtctttttca gccaattgtt gtgcaatgat
     4081 tcttgttgct tcattcatca cagtataata caagaattct ctgtcagtct gttctgcgat
     4141 ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt gcttcttcgt attgttcttt
     4201 cgtaatttgg ccttcacgat acatactgaa taaaacaaaa tcttttctgg caaggccggc
     4261 agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga ctatacgtga tcgggctttg
     4321 gggtaatcca gcgatataag cagcttgcgg cagcgtcaca tcttttgcat ctacaccaaa
     4381 gattcctttt gcagcttcct ggactccagc aatgttttgt cccttattat tacgaccaaa
     4441 tggtgagaca ttcaaatacg tagcaatgat ctcatctttt gaaaagtatt tttctacttg
     4501 tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt gtttcatctg taaggatctg
     4561 ctgtttgact aattgctgtg tcaaagttga tccaccagaa gagccaatac cagtagcttc
     4621 agatactaat gctcgtaaaa cagctttagg aacgtaacct tggtgtttat cgaagtattc
     4681 atcctctgtg ctgataattg cagttttcaa taaaggtgaa atcttatcag atgaaatagt
     4741 cgtgcgcatc agatctgtct ggatttttga tatctctgta ttatctgcat aaacaatctt
     4801 tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct tttttagtcg gcaattgtgt
     4861 gtcttcgacg agataggcaa aatagccagc gcctattcca atacctagag ctgcgaacat
     4921 caaaagaaga acagtaataa aaacagtcaa tgattgaaaa actctaaaaa taatcttagg
     4981 aacaaaccat ttttcttttt ttcttctatt ctgttttttt cgagtatttt tgcgtggcaa
     5041 aagggtcacc tcaactaatg gttttatttc gctaattata gcaaataata gaacaaaaaa
     5101 acagacaagg ctgtttttgg caggaatttc ttttttaaaa gagttaaaac gtgagaaaat
     5161 gatctttatt ataaaagagt tcttaagctt ttctttagag aaaatttcag ttactatgga
     5221 gaacagggca aagaaaaatc aatcattttc tttgtatatc atcttaccaa tatataggaa
     5281 ttgttagtaa caattgcctt tcaaggagga atccatccat gttattaggc tcacacgtca
     5341 gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga agcagccagt tatggatctt
     5401 cgacttttat gatctataca ggcgcccctc aaaacactag aagaaaagcc atcgaagaga
     5461 tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg tctaacaaat atcgtcgttc
     5521 atgcccctta tatcattaat ttggggaata cgatcaaacc agaaatgttt ccttttgccg
     5581 tccaatttct gagagaagag attgttcgtg cggaagcttt aggcgctact caaattacac
     5641 ttcacccagg tgctcatgta ggggcaggaa cagaagccgg aattgctaga attattgaag
     5701 gcttgaatga agttttgaca aaagaacaga cggcacaaat cgctttggag acaatggcag
     5761 gtaaaggtac ggaaatcggt cgaacatttg aggaattagc gcaaatcatc gatggagtaa
     5821 aattgaatga aaaattatcc gttacttttg acacctgtca tacgaatgat gccggttata
     5881 atattaaaga agacttttca ggtgtaatgg aagagtttga taagatcatc gggttagatc
     5941 ggcttaaagt actccatatc aatgattcta aaaatccgca aggttcgcat aaagaccggc
     6001 acgccaatat tggttttgga acaatcggat tcgacacgct gaataagatc gtccatgatc
     6061 caatgtttga caatatttcg aaaatcttgg aaactcccta tgtaggcaaa gataaaaaga
     6121 aagcttacgc accatatgga aaagaaatcg ccatgttcaa agcgcaaaca ttcgatccag
     6181 aagtgtttcc tgagttatta gcagaagaag tgatactcta agattttcta tctgacagca
     6241 gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt taagaaacaa aggagtactt
     6301 tttgcagtct ttttcatcat gcatcttctc ccccctcttt ttaaatttag acaaacaagg
     6361 tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag caagtggcaa aacgtgattg
     6421 gaaggacgaa aagatgctga acactttaaa acccttgatc aaccaactaa aaaagaaaca
     6481 tacatacttt attcttggca tagccctatt atttacaatt attcgtttgt atttatatag
     6541 taaagcaatc tatggaacag acatgtcggc aacctatgat gaccagctgc tgattcgcta
     6601 tgctcaaaat attttggacg gtaaatggct aggggagtat acgactacaa cactttccaa
     6661 aggaatctcc tacagcttat ttttagtact tgcacataaa ctgcatcttt cttatgcagt
     6721 tttgttcggc atgttcagta tttttgccag tatattggtt gcactcgcgt ttaaaccgat
     6781 tgtgaagaac aagtatcttc ttttaagtat ctatttttat tttttgtttt ctccgattag
     6841 ttttacacat gaatattcca ctcgaacata tcgtaatgcc ctcgtgattc cggctgttga
     6901 gatcattatc gcttgtttat tagctattta ttaccgcaaa tacggttcta gcagaaaatt
     6961 gctgccatgg tttttagggc tttctgtcgc ttttcctttc ttttggttca ttcgcgaaga
     7021 ttctatctgg cttcttcctt ttactgtagt agctttgggt atcagtttcg tccagatctt
     7081 gctagggcaa gacttttctt tcaaagaagt aaagaagtta aaaaaacgac tctttcttct
     7141 ctcgaaacgg gaatggctga aactttcatt gtttttactt cctatagttg gcttttccat
     7201 cgtctatcaa tggatcgaat ataaaaatga gacaacatac ggtattgcaa ccatcaatga
     7261 ccgttccagt ggagaatttg gacgtttgat gaaacaactg atccgaatag atgatgaaac
     7321 gaacttgaat aaagaaaaca gtgagatatg ggtctcacat gctgcactaa ataaagcatt
     7381 agccgcatct cctacattcc atactgtttc tacccgtatt gacgaattat atacgactca
     7441 cgcgtggact caaggtggaa aagtaaaaga attacccgga gatatcatct tttgggcatt
     7501 gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa ccaatgattt
     7561 ttggaaaaaa gtcaaccaag aattagaaga agcctatcac aagggaacaa tcaaaaagaa
     7621 aaaagagttc tatctgatgg gatcaggaga tggaaagacg atgagtgatc tgccaatcgt
     7681 attcgatttc cttcaatcag gatacgctta tgcatggcga tataaagatt tccatcaagg
     7741 aggaaattat agtttcggaa cgcaagaaca agtcaaacag gctcaagaat tattaaacct
     7801 tccgctaatg gagaattgga tcgatagtaa tgaaccgaga acacctaatt tccaattaac
     7861 aaaatcagca aaagtcgcga atgtcatcat aaaaatttac caagttctgg ctattcccgt
     7921 gctgattgct tcaatagcag ggatattact tatgatacta ggaaccgtat ttgcaaaagc
     7981 tgatcgtgcc tattatagtt cctctttgat tgtggtcacc ggattaatac tgacacaatt
     8041 ggtttttctt ttcggtgtct catggttttg ttcttatgcg ccagaaaaaa aggactattt
     8101 tttaagtgtc tacactggtg caggcgtgcc attaacgcaa ttcatcgtag taacaagcat
     8161 gctagttttc agtaaattaa atctgaaaaa agctttatct tctaaataaa aagacccttg
     8221 gtagctagat ttttaaccta actatcaagg gtctttgtgt ggaaaaatca gtttttattc
     8281 gatttcttct accgtttcct gcaaaagctg ttcatatttt ttgtttcctt tatataaatc
     8341 accaatcgtc catgccaaca ggagaaggac aagaacaatg attacataag cgatgtgatc
     8401 agccaatgct agttgagcgg aagtggcttg ctcgccgaag aaactttcga tctgtcctgg
     8461 taatcctcgt aagttcaagt aagttagact aatcacagag aaccagccaa gaagttttac
     8521 gatgaatgaa tttttaaatc ggctacccat ctccgcttgg ctgttggtca tcatcagtaa
     8581 aggaatcatc gaaaacggca acgcaaaagc caagaacacc tgggattcat tcattaagtt
     8641 attcaaagcg gcatgttctt cgatcgttcc ttttgtactt gtaaacaaaa cacaaatcaa
     8701 tacaggaatc acagaaagca atcgtgtgac cagtcttctt agccaaatcg ggatacgcat
     8761 atggatgaat ccttccatga tgacttgacc ggtcaacgta ccagtaatag tagagttctg
     8821 gccagaagca agaagtgcta cagcaaataa agtggacaag ataccagatt ttgctacagc
     8881 aatcaaaata ccattgctca tagctgatgg atcagataaa gcttcataca gaccaaaaaa
     8941 ggaaggatct tggacagaac cggttttgaa gacagcgaca cccatgatca atagcagtgc
     9001 attgactaga aaagccattg acaactggat attagaatcc caagtagaaa aacgtacagc
     9061 ttgtgctaca tcttcttcgt tttggtgatc gatcttccga gtttgtgaaa ctgctgaatg
     9121 aagatataag ttatgcggca tgactgttgc accaataatc cctaaagctc cagtcagcgg
     9181 agtttcgccg ccgatgaccg gtgagtctga gaatgtatga ctgttaggga taaagccagc
     9241 aaataaactg tgccaatctg ggttagacaa gccaacttga tagacaaaaa cgaataaaat
     9301 gacgaaaatt aagcagacaa ccagtgcttc gatcttacga aacccgactt ttgtcagtag
     9361 taagagaacg aaaacatcta atacagtaat aaagacggca atcaccaatg gaatatcaaa
     9421 aagtaagtag agggcgattg ctgcaccaat gacttctgca atatctgttg ccataatggc
     9481 taattcagtc aagatccata aaatgacacc taatgtctta cttgtgcgtg ctcggattgc
     9541 ctgtgccaaa tccatttggc tcacgattcc taattttgct gccatatact gtaaaagcat
     9601 agcaatcaga ctagacatca aaataactga catcaataaa tattggaaat tttgtcctcc
     9661 ggtgatcgaa gtagaccagt tacccgggtc catataccca actgctacta acgcaccagg
     9721 tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc tttggtacat caactgttcc
     9781 atttacttca gctaaagata aaccatttgt atgttcaata agtcgatgct tatgaaattg
     9841 tttattggat tgattattca agaaaaaacc acctttccta tttatctacg attacattct
     9901 acacctcaaa aagaaaaaat taaagaaaaa gattcggtac gcctaatttt ttttcatggc
     9961 gcgtcttgct agatgacgaa agaaattttg ttctcctcta aaaaaaccac tattttttgc
    10021 atgaaactaa agaatacgcc tttctttttt caataaactt tagtaaaata agtcattgag
    10081 agcaattcgg aaatagaaag gagagattta tatgttatca acaggtattg tcgtacttat
    10141 tgctattatt gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc gtaaatatat
    10201 gaaagactat ttagaaaaaa atcctcccgt taatgaggac atgttgcgat caatgatgat
    10261 gtcaatgggg caaaaaccat ctgagaagaa aattcgccag atgatgcagc aaatgaagaa
    10321 ccaagggaaa aaataggttc tgacccaacg actttttacg ggctgatttt ttcagtccgt
    10381 tttttatttc gtaatttgtt agactattca gattattcag caaaaaagac aagcacagac
    10441 aatcctaggc tcttgataaa gacaaaggag gagtaaaatg tcgattttta gaaaattagg
    10501 ctggtttttt cgtcaggaaa agaaacatta tttaattggg gttttctcgc tattcgcagt
    10561 ggccttggta cagttagttc ctccaaaagt gattgggatc atcattgatg agatcgccga
    10621 taaaaattta tcaataaaga tcattttcgg gtggatcttc ctattgatta tcgctgcttt
    10681 tttacaatat ctttttcgtt atatctggcg gacgaatatt tggggaagtg cagctcgatt
    10741 ggagaaagaa cttcggcagc agttattcaa acattttaca gaaatggaca atctctttta
    10801 ccaaaaatat cggactgggg atttgatggc tcacgcaacg aatgatttga atgcgatcca
    10861 gaatgtagca ggagctggta tcttgacctt tgcagattca gtgattacgg gcggaacaac
    10921 gatcattgcg atgattttgt ttattgattg gcgcttaaca ctgattgcgc tgattcctct
    10981 gcctttgctg gccgtcactt cacgagtgct ggggtcaaaa ctccatgatg cttttcgtga
    11041 ttcgcaagaa gctttttcta gtatcaatga taagacacaa gaaagtatca ctgggatcaa
    11101 agtcattaaa acatttggac aagaaaaaga agatctagcg gattttgaag aaaagattga
    11161 agatgcaatt attaaaaaca aacgagtcaa ttttttagac tccttatttg atccattcat
    11221 taccttgatt atcggtctgt cttatgttgc aacaattatt ttaggtggaa agtatgtctt
    11281 ggaaggcacg atctcgatcg ggcagatggt ctcatttatt agttatatcg gtatgcttgt
    11341 ttggccgatg tttgcgattg gtcgattgtt caacgtattg gaaagaggaa atgcttctta
    11401 tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc gtggaacaac tgggtgcagt
    11461 gcagaccccg gcaaaaggcg agctgaatgt agctatttcc tcttttaatt atccgaaaga
    11521 agaatcacca gcattagaac aaatcaagtt tgcgatccaa gaaggagaaa cattaggtat
    11581 tgttgggaaa actggtgcgg gaaaaacgac gatcatcaaa ttactgatgc gtgaatacga
    11641 tgattatcaa gggaaaatcc aatttggcgg acgttcgatc aaagaatatt cattgaatgc
    11701 tttattagga tcaatcggct atgtgcctca ggatcacttc ttgttttcga tgaccattcg
    11761 agataatatc cgtttttcta atccaaaact ttcagaagaa gaagtagagc atgcggcttc
    11821 tttagcattt atcaacaatg aaatcaagga attaccaaaa ggttatgata ccttagttgg
    11881 agaacgtgga gtctctttat ctggcggaca gaaacaaagg atttcgattg ctcgagcatt
    11941 gatcaaaaat cctgaattat taatattaga tgatgcactc tctgcagtag atgccaaaac
    12001 agaagaagct atcttgcata atttaaaaga aataagaaaa gagaaaacaa ctatcattgc
    12061 agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc attgttgtcg atcaagggaa
    12121 aatcattgaa cgaggaaccc atcatgagct gcttgcacta ggcggctggt atgaacaaat
    12181 gtgggagaaa cagcaattag aagcgaagat agaaggaggc gaggcctgat ggatgaaaat
    12241 tatcaatcgg aatggacaaa atcgattcca cttaaagaac agaaaaagat catcaaacga
    12301 ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg ttgctatctt atttgcgttt
    12361 gctttgtctg tgatcaatgt cctattgcca aggatcatcc aaacttttat ggatgatcat
    12421 ctggctaaac agtcggctac gacacaagtt attttgtttt ttgctggtat gtacttattt
    12481 ggagtcatag taaaaagtat catttggttt ttccaatggt atttgtattc catggcctca
    12541 ttgaagacct atcagtatat tagggtaaaa ttatttgaaa agctgcatac actaggtatg
    12601 cgttattttg atcagacacc ggcaggatca accgtttctc gagtgacgaa tgacacagaa
    12661 acgctatttg aattttggta cgtctttttg atggtattga ctgggatctt tgcagtcgtt
    12721 tcttcattcc ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac tttggtgttt
    12781 ttgccgattt taggaatcgt catctggtat taccagaaat tcagttctcg tatttaccga
    12841 aatatgcggg aaaaattaag tcagctgaac acgaaattga atgaatcgat ttccggcatg
    12901 cgtattatcc agcagtttcg ccaagaagct cgtttagcta aagaatttga acaagtgaac
    12961 aatgagtatt tagacacgcg ttatgcgatg atcaaaacga attcgattct gttaagtccg
    13021 atcatcaatt ttctatatgc attggcgatt gcgttggtgt tgactttgtt tgggatcgat
    13081 gcgttgcatt ctcccgtaga agttggactg atctatgcat ttacgaccta tgtccaggga
    13141 ttttttaatc caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt
    13201 gctggtagtc gtatcttgaa gattatggat acggaagaat atgcaccaca acaaaaccct
    13261 ggagcagatc aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat
    13321 gatggagagc acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt
    13381 gcattagtgg gacacaccgg aagcggaaaa agctcgatca tcaacgtatt gatgcggttt
    13441 tacgaatttt atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg
    13501 gaagagttac gtaagaaaat ggggctggtc ttgcaagatg ctttcttgtt ttatggggat
    13561 atcgcaggaa atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg
    13621 gctgagtttg ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa
    13681 gtgatcgaac atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga
    13741 acaatcgtga ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact
    13801 gaaacagaag gattgatcca agaaggattg gcaaaaatgc gtcaaggacg gacaacgatt
    13861 gcgattgccc atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa
    13921 ggtcatatag tagaacgcgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca
    13981 gatatgtaca aactgcaaag cagtggtata taaaagatgt tgtgacagta gaattttgac
    14041 ctaagcacta gagtgaaaat aaaacatagc ttctcatatt tttcatctgt gagagttagt
    14101 gtcgtagtca ccagatttaa aaaaagattc gacagctagt cagaagtgcc taatagccac
    14161 taagaaagcg ttgacaaaag catttgcttt cgataatata aagaaaacga tacaacttgg
    14221 atagtgattg cctgatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt
    14281 tttgggtttt tctttttgtt ttggatacag ccctatacat catggaaagg agcaaaacat
    14341 gctgattgag aaagtactga ataacaacgt cgtaataacc agggatgaaa gaaatcagga
    14401 aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga
    14461 ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg ctttcacaac agttccaaga
    14521 attggcacaa gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac
    14581 aaaagaggct ctacaagtcc agttgaatga ttctgtgtat attgcattga cggatcattt
    14641 gtatacggct atcgaacgtc acaaaatggg aatatttgta ccaaatgtat tgctgtatga
    14701 tatcaaacaa ctatttccta aagagtatgc catcgggaaa aaaacagtga agaaaatctc
    14761 ggataaatac cacatcaagt tatcagaaga tgaagccgga tttatcgcat tgcatttagt
    14821 caatgcacaa ggcattcaaa gccagcaaga caatacctac ctgtttacta taacaataca
    14881 ggatattcta aatatcgtcc gctattacta taagattaca tttgatgaag attcagtcca
    14941 tttttttcga ttcgtcacac acttacgttt ttttctgaca cgcgtcctag atggagaaaa
    15001 atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt attcaaaaaa aatataagga
    15061 tacgtttggt tgcgttgaga aaatagcaga ctttttagcc gagacgttca actaccagat
    15121 gtccacggac gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg
    15181 ttgtaaataa aaaaaggagg ataaattaac aagaacgtat gaaataaggg tagtgacatt
    15241 cagttggcta aaccttccac taataaattg gagggaatag aaatgggaaa ataccaagaa
    15301 ttagcgagaa aaatcgtaga aaatgtcggc gggaaggaaa atatcaatgg tttgacacat
    15361 tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa aagcaaatga tgagatcttg
    15421 aaaaatatgg acggtattgt aacggtaatg cgagcgggag ggcagtatca agttgtcatt
    15481 gggaatcatg tgccagtcgt gtttgaagag gttataaaag caggaaacct tacttttgat
    15541 gaggctgttt caacagaaaa aatgagaccg tttgatatgc ttattgatgg aatatcgggc
    15601 tgtttccagc cattccttgc tatcctagct gctggcggga tgattcgtgg actgacagca
    15661 ttccttgtct tcttaggcgc gtttgaccgc ggttctggca catttgttat gtttgataat
    15721 atcggtgatt ctgtattcca atttatgcct gttattatcg gtttaacagc tgcccgtaaa
    15781 tttaaagtaa atgaattcgt agggatgttg cttggcgcag cattaatgaa tcctagcttg
    15841 tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa cgaccatatt ttctggcact
    15901 atttttgaag cacctattta tcaaacagta ttcggcattc cctggattgc gcgtaactac
    15961 gcttcttctg tcattcccat tatctttatt gtgttgttag ctagccaaat tcagaaacca
    16021 attaaaaagt tagtacctga gatgatcgcg aatttcttcg ttccattctt caccgttctt
    16081 attacaatgc ctttaggttt tttactcgtt ggaccagtat tcacattcgc aactgatatt
    16141 ttaatggctg gatttgaaac acttttagca ctttcgccag ttatttatgg agcaatcgta
    16201 ggcttcttct ggcaaattct ggtgatgttt ggtctccatt gggcaatcgt accaatggga
    16261 ttgatgcaat tttctgtgaa tggttggcaa aatattatga cacctgttgc agtagtgtct
    16321 tttggacaaa ctgcagcact taccgcactc tactttaagc ttagaaatcc aaaagacaaa
    16381 gcaattgcaa taccagccat tgtttctggt attgtgggga ttacagagcc agctatttat
    16441 gggtttacat taccacgtaa aaaaatattt atttttacct gtgtcggtgg tgcgttaggg
    16501 ggcgcgtata gtgggttaat gaatttaact tcttggaatc aaggtggact aggcatcttt
    16561 acaattccca actatattag accagatggt gatttaacag atgtgattaa tgtattgatt
    16621 gggatcgcca ttgcgatggt ggtatccttt acgctgactt ttttcttttg gaaggatgaa
    16681 gcaggtgaaa cagatgatat acagaaaaaa
//
LOCUS       DACGLH010000060        16324 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.064, whole genome shotgun sequence.
ACCESSION   DACGLH010000060 DACGLH010000000
VERSION     DACGLH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.064"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(109..765)
                     /locus_tag="IXT21_002064"
     CDS             complement(109..765)
                     /locus_tag="IXT21_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ4869448.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            958..1236
                     /locus_tag="IXT21_002065"
     CDS             958..1236
                     /locus_tag="IXT21_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869449.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1255..2463
                     /locus_tag="IXT21_002066"
     CDS             1255..2463
                     /locus_tag="IXT21_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4869450.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2611..2976)
                     /locus_tag="IXT21_002067"
     CDS             complement(2611..2976)
                     /locus_tag="IXT21_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ4869451.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3123..3896)
                     /locus_tag="IXT21_002068"
     CDS             complement(3123..3896)
                     /locus_tag="IXT21_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4869452.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4070..4930)
                     /locus_tag="IXT21_002069"
     CDS             complement(4070..4930)
                     /locus_tag="IXT21_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ4869453.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4930..6348)
                     /locus_tag="IXT21_002070"
     CDS             complement(4930..6348)
                     /locus_tag="IXT21_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4869454.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6405..6854)
                     /locus_tag="IXT21_002071"
     CDS             complement(6405..6854)
                     /locus_tag="IXT21_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4869455.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7299..7562)
                     /locus_tag="IXT21_002072"
     CDS             complement(7299..7562)
                     /locus_tag="IXT21_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ4869456.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7661..8956)
                     /locus_tag="IXT21_002073"
     CDS             complement(7661..8956)
                     /locus_tag="IXT21_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ4869457.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8953..10224)
                     /locus_tag="IXT21_002074"
     CDS             complement(8953..10224)
                     /locus_tag="IXT21_002074"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ4869458.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIEDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTIGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10389..11258)
                     /locus_tag="IXT21_002075"
     CDS             complement(10389..11258)
                     /locus_tag="IXT21_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ4869459.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11550..13160)
                     /locus_tag="IXT21_002076"
     CDS             complement(11550..13160)
                     /locus_tag="IXT21_002076"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ4869460.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14353..15693)
                     /gene="glnA"
                     /locus_tag="IXT21_002077"
     CDS             complement(14353..15693)
                     /gene="glnA"
                     /locus_tag="IXT21_002077"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ4869461.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15721..16113)
                     /locus_tag="IXT21_002078"
     CDS             complement(15721..16113)
                     /locus_tag="IXT21_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4869462.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4660 a         3462 c         2772 g         5430 t
ORIGIN      
        1 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgtttgt
       61 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttgttcatc aaaagataat
      121 attgtcttct aacaagtcca tattcaaaat aacgatatga tggtctttaa cggtgataat
      181 accatctttt ttcagttgtt ttaaaattct gtttacactg ctagcagttg agatgccaca
      241 aaatttggcg atttcttcat tcgtgatcga aaaatcgatc agcatccctt cttctgtccg
      301 cacaccgaat aaatcatata attcgtaaag ttgcgtacaa acagctccca gctttccgtt
      361 taatagcatt tgctgcattt ttttcatcga ataaagcaga cgatagcggt aatactcttt
      421 tacatagtgt tgtagctttg ggtcttggtt gatattttgc caaaagcgca cacgattgat
      481 tcgataaaac tctgcatatt ctgattcaac tcgaatatta tacggcgaat cgatgaattg
      541 tgaatattcg tcttttaaaa gagagacgat ttcaagatct ttgatatagc gaaggttaaa
      601 ttctctccca tcatttgaaa taacacttgc ttttaccgtt ccctctttca agatatacgt
      661 atgggaatct tccatccctt cgaatgtcaa atatttatga tatcctttgc gtataatcgg
      721 aaaatcattt ttatccaaat attcctttaa gactctacta tccaatgatg ctcccccatc
      781 caaactttca tctaaaaaga tcatacagta ctttaaaact ttttaaagtc caaaggtatt
      841 ttactaatga tattaacatt tgttaagtac ctttttccat cacttatgcg atttttttcc
      901 ttttttttat tcgttatact gcagaagtaa aattttttaa gaatagaggg aaaaaacatg
      961 aattactgga tgaacacaat cattaatcga ttagaaacag catatcaaac aagatttgat
     1021 atgaaagctt ctttagtctt tttaaatgat gcgtatcaaa actcgatcga attgatcaaa
     1081 gcagtggatg agaatccaac aaacgaatgc gaagaatttt taaatctgtt tatgtctaca
     1141 agagatttat ttattcgcca acttgttgat cgctatccat ccaattatca cgatgtagaa
     1201 gttcaaatac aaaaattaaa agcatattca gcttaagggg aatgtataac atacatggaa
     1261 aagaaattat ttaataaata ttttattgga atcacattga tcaattttat tgtctacctg
     1321 atttattatc tgttgatggt catcatggca gtcattgcgc aagacacctt acacgccagc
     1381 ttgagccaag caggttttgc ttctgggatc tatatcatcg gaacattgct ggcacgctta
     1441 ttcatgggga aacagttaga actatttggt cggaagttta ccttaagagg cggcgcgatc
     1501 ttctatctcc tttcaacatt agcatatctt tatacgccaa ctatcggggc attgtattta
     1561 gttcgattct tgaatggatt cggctacggt acagtttcta cagctacaaa tgctatcgtt
     1621 actgcatata tccctgcatc taaaaaaggg gaaggcatca attattatgg attaagtact
     1681 agtttagctg ccgctgttgg gccattttta ggaatgattt tgctgaatat gactgatttt
     1741 cgtttcatta tctggttttc aattgtactg gtcttctttg ttacgattgc ctgccttgtc
     1801 tttcctgtaa aaaatattcc attatctgaa gaacaaaaaa tatctcttcg ctcatggaat
     1861 atcaatagtt ttattgaaaa gaaagcactt ttcattacag gaattgcctt tttgatgggt
     1921 ctttcttatt caagtgtttt atcattttta tcttcttata caaaagtaat ccacttagtt
     1981 gctgctgggt catttttctt cgtcgtatat gctttggtca tcacatttac acgtccattg
     2041 actggaagga ttttcgatgt caaaggagaa caatacgtga tgtatcctag ctatttattc
     2101 ttaacagcag gattattttt actgagtgta acgacgaata gtgtgacatt gcttatctct
     2161 ggagcattag tcggcctagg ttatggtacg tttatgtcaa atggacaggc tgtttgttta
     2221 aaaatcgtcc gcgaacatcg aatcagtatt gctttatcta cctactttat tggtttagac
     2281 ctaggcttag gcgttggtcc ttatttgatg gggatgctaa aatctcttac ttcatttaga
     2341 ggattgtatg tgattgccgg agtcattcca ttgatatgta cagcactcta tcttgttaat
     2401 ggccgaaaac atagtcatga agcagagggc gaattgattg aatcattgaa aaatgaaatt
     2461 tgatcttgat gaatagataa aaaaaaccgt tcagatctga aaataatctg agcggttttt
     2521 tgagtaaaaa tagtttatga ttattcttaa ttaaacatct atttattttt aaagctttca
     2581 tttatcgata agaatagttt aactgttgat ttattccatt ttccgttcat ctggtacgat
     2641 gccttgactt agcaaccact tgtggaagaa ccattctgcc acaccgatca atagagcagg
     2701 aatcaatact ttcatccaaa tatcgactgt atcattcata aaatatagat agagccacaa
     2761 gataactgca gctgaaccag catctgcgat cgtcgctgcg atgttcccta ctttacgtaa
     2821 gatcaacaga tcccctagca catagatcat gactacaata attaccccaa caatcgttga
     2881 atccataaag ctaacaccgt ataaacctgt cagcacgacc cacagaatcg cccagatcat
     2941 gattgccttc actaccaacg ccttcacatg tttcattttc tgcactcctt tctcctggat
     3001 aaaaaagtaa gattatctaa caagataacc ttttcattat tataccatcg gctgagagaa
     3061 gcgagcaaag ataaggtcct atctaaaaat gaggaagcca cttagaacat tcacgtaata
     3121 atttatccgt acagacattc gatatggtgt tcctttactt tcgcatactg ttcttttgtc
     3181 agttctttac ttgtcaccag ataatcgatt tcatgaaaag caatggcttg atgcatgaat
     3241 ttatgtgcaa ttttttgctg tgtaactaaa acggcaagcc ggtctgattg ttttctaaaa
     3301 gcttttttga ccattgcctc tccttcatga gtttctgtta ctccttggct gaaatctagc
     3361 ccgcgacaac tcattatcgc taaatccgca taatatgaca atgcatcgtt ataggctttt
     3421 gaaccgttga ttgttgctcg tttcggaaca attgcccctc ccaatacaga tacttttgcc
     3481 gttgtatatt cactcaacat agcagctgtg acgatgccat tcgtaatgat ctgcaaacca
     3541 tcaaatcttg ctaggtttct gactaagtgt aaagaggttg tactggaatc caagaataag
     3601 accatatcat ctcgaatcaa agaagaagct gtatctgcaa tcttacgttt ttctgtttct
     3661 tcttctaatt tggtgatctt catcaggtaa ggcacgtcaa tttttcgatt gtcaataagc
     3721 atggcgccac cgcgaactct acggatcaat ccactttttt ccagaaaaac aaggtcacgt
     3781 ttaatcgtgg cttcgctata atttactaac tcgatcaaat cggtgactct tgcaaattgg
     3841 ttgtttttta gataattcac aatctcctgc tgtctattct ccacttcatt caacataaaa
     3901 tcaccccatt tactttatat agtaacagga aaaaagaggt tgtgacagaa gtgattggct
     3961 tcgagaaata agaagaaatt tttcgaaaaa actacttctg aacatcgtgt gtggttaaga
     4021 gggtatgaca agactattgt cataccctct tgaccacaca tttttatttt tagtataaac
     4081 tagccttgcc tactgtgttc aaaatttcca ttttatgttt gaccacttct tgtacctttt
     4141 tattagctgc tggataaatt gcattcggct cgtacatcga tggattttca ctcaatattt
     4201 ctctcaaagc attgaaaaag acgcttttaa gatctgagct caaattcact ttggcaactc
     4261 cgtattttac tgcttcgctg acttctgcgt ccggattacc tgaaccgcca tgtaaaacaa
     4321 atggaagatc gatttgttgg ttcaattctt tcagtaagtc gatatttaat tttggagttc
     4381 tgtttttcgg atacaagcca tgcgctgttc caattgctac agcaagtgta tcgattcctg
     4441 tttctttcac aaaaatcttc gcttgatcag gatctgtata aatgattgtt tcagcacctc
     4501 cttcagcaga accattattc ccaatcgttc caagctcagc ctccactgag acatttaccg
     4561 catgagctaa cgcaaccact tttttcgtaa tctgtacatt gtcgtcgaat gatttcaaag
     4621 acgcatcgat catcacggaa gaatagccgt ttcgaatcgc ccgtaagaca tcttctatcg
     4681 taccgccatg atccaagtgg ataacgatcg gcagtttact tgtataagca taggctctta
     4741 ctgccgcaac aaactcatca cttaaatagg cgatttcatc aggatgaatt tctaagatta
     4801 ctggtgcttc taatctctct gcttcttcga tgattgcttt catcatatca tagctgcaaa
     4861 tattgaaggc tggtactgca aattgattct ctttagccac tttcaataac tctttcattg
     4921 tatataacat tacgcttcca cctcatttgt tagattttca tttttcgtcc acttgtcttg
     4981 ttttttctct ttcactgttt ttttcaatag tccaagcata aaggctgtag tgactgtacc
     5041 tgatgcaatt gccaagacat acataggtaa attgttcata acatttggaa ttagcatcac
     5101 ccaaatccct ccatgaggtg ccattgaagt aactcctact gccatggaaa tccctgcagc
     5161 gactgctgaa cctgtcatca aactaggtat cacgcggatc gggtcagata ccgcaaacgg
     5221 tatagcacct tctgtaatat acgaagcgcc taaaatccag catgattgac ctgaagtccg
     5281 ttcttctttg gtaaatttat gtttgaataa taaagtggct aaagcaatac ctaggggagg
     5341 tgtcattcct gcagccatac aagcagcaat cggtgcattt actccagctg acatcaaacc
     5401 gatcgagaaa gtcgaagtcg atttattgat cggcccaccc atatcagctg ccatcatgat
     5461 cccgataagt aatccgaaca atagcccgga tgtatttccc aagctattca gccaatttgt
     5521 caaagcatcc aacacccatg tgatcggttg ttcaatcaca aataccatca aaagcccaat
     5581 aattaagatc gataaaaagg ggacgacaat cagctgataa atagcagaaa attctttttt
     5641 tacgctaatt ttatttgcca gaaaatcaca gatataccct gctagcacgc caccaatcat
     5701 tccgccaaga aaacctgaac cacctgtaga ggccagtaat ccggcaacaa ttcctggggc
     5761 aaaacctgct tttccagcaa tcgatgtagc aatccccgcg cctaacgcag gaaccatcat
     5821 ggcaaacgct gtatctcctc cgatccttga aaatgcagct gctaacacat tatagtctgc
     5881 tgagttggga tcagccgctg taatcccaaa ggcaaaactc agtgcgatga tgattccgcc
     5941 tgcgatcaca aatggaagca tgtaattcac accattcatg aaatgattat agattgtcgg
     6001 ctttttctca tttgcagctg gttctttttc attagacaca acgaatacgc caatatctct
     6061 caatgcttcg tctattacta cttgcggttc tttgattgct cggcttgtag agacacgctt
     6121 tacttttttc ccggcaaatc ggtccaattc gatttcttta tctactgcaa gaatgattgc
     6181 atctgcctct ttgatttctt gttcagataa ctgattttct gtttttacac cttgtgtttc
     6241 gaattttgct tgatggttta gatttcttgc tgttacttct aatttttctg ccgacatata
     6301 agtatgagca attccagtcg ggcaagcagt tacaccaacg attttcatga ttattttcct
     6361 cctttttctt cactatcgat actcggacaa agactctact attttcattc ttgactaatc
     6421 tgtctgatga tatctgctgg cgtagtggct tcttttattc cctttctaaa atctgcattc
     6481 atcaatcgtc tggagagtgt gctgagaatc tctagatgca tatctgctga tttttcagga
     6541 actacgatgg caaaaatata ttcaattggc atttcatcta tcccgctata gtccgttaat
     6601 ttggctgtat gaaaaaaaca cagtgctggt tttgtcacat atttgctttt tgcatgggga
     6661 atcccaaatc catcttctat tcctgtggag atctcctcct ctcgtttgaa tagtgcctta
     6721 acgacttttt gaacatttct taccttattt gtttttttca acacatcccc tattcgctca
     6781 aataatgatc cttttgaatc tatcgatgga tcgaaaacaa tcagttcttc tgcgatcaat
     6841 tgatcaaacc tcataaaaaa cctctccccc acaaaaaata ttaacgtttt cttggaactg
     6901 aggtcagtat agcatcgcta gctgtaccgt tcaatattat gaatgaacag aggaaaaaga
     6961 cccatcatcc gcgtgtataa gtcattaaaa tgaacgatct agctcacaaa caatcgcaag
     7021 tctataacaa aacaaaaagc cttccttgaa aggaaagctt agcttctaat tttaggtaaa
     7081 tggtttttta cttcgaaata acccctcttc atttcgttaa tataaggtgt ttttatcaag
     7141 tggataatta aaactatata ctagtttttc attgttttgt gcattatgat cctacgactt
     7201 tttcagcatc aaacgaagtt ttaaattggc aaaaaacata aaaaaacaga ctatccctat
     7261 cagatagtct gctaaacgtt gagttatcag cgtttttatt attccgcagc gtttgcgtct
     7321 ttgagaccgt attttttgtt gaaacggtcc acacgtccgt ctgcttgtgt gaatttttga
     7381 cgtccagtgt agaatggatg tgagtcagaa gttacttcga cacggatgac tgggtatgta
     7441 ttaccgtctt cccattcaac tgtttcttgt gaagtttttg ttgaacctga caagaatttg
     7501 aaaccagttg ttgaatccat gaatactact gggtggtaat ctggatgaat gttttctttc
     7561 atgatttttt cgctcctttg ccctgctcca tttgctgaac cagagttgat ttgtcgattt
     7621 acaacattaa catcttaaca tgtttcagtc aaggttgcaa ttatcttttc agatttctgg
     7681 tcggattttt tttaccaaag gagacatctt gaaattcttt gaaaaattgc tgattgtttt
     7741 ttgttttacg caaaaagcgg atcagttgtt ccgtatattc taatgaatct ccagtcatat
     7801 tttttcgaag tttccaaatt tcttctaatt cttcacttgg catcaataat tcttcttttc
     7861 gggtacctga ttttttgata tcgatggctg ggaagatccg acgttcagat aattcacgtg
     7921 aaagttgtaa ttctagattt ccagttcctt tgaattcttc atagatcacg tcatccatac
     7981 ggcttcctgt atcgactaaa gcggtcgcta aaatcgtcaa actgccacct tcttcgatgt
     8041 ttcttgctgc cccgaaaaat ttcttaggtt tatacaatgc ggctgggtca atcccgccac
     8101 tcaatgtacg tccgcttggt ggaacgacta agttataggc ccgagcaagt ctagtaatac
     8161 tatccatcaa gataacgaca tctcgtttat cttcgactaa acgcattgcg cgttccaata
     8221 ctaattcaga aactcgagta tggttttgcg gctgcagatc aaatgtagaa gataccacgt
     8281 cgcctttcac gcttcgttct agatccgtta cttcttccgg tcgttcatca atcagtagaa
     8341 cgattaattc tacttccgga tagttttcgg tgataccatt tgcgatttct tttaatacag
     8401 aagttttccc tgcttttggc ggtgcgacga tcaatccacg ctgaccaaaa ccgattggag
     8461 aaaagatatc gatcattcgg gtagatagtc tttgcggtgt tgtttccagt tgaagctgtc
     8521 tttgcggata aagaggtgtt aaggctggaa aatgcggacg ttcttttgct tcttccggat
     8581 cttttccatt aacgctttcc acgtgcatca aaccataata acgttccgat tctttaggtg
     8641 gacgggcttt tcctgccact ttgtccccat ttctcaaact gaatcgtctg atttgtgagg
     8701 cagaaatata gatatcttct gcacttggtc cataattgat tggccgtaaa aaaccgtaac
     8761 catcttgacc aacgatatcc aatattcctt ccatataata aaatccctgt ttctctgctt
     8821 gtgcacgaat cacagctaga gataattcct ttttattcat cttactgtaa taaggaattt
     8881 taaattcttt ggcataacta taaacttctt ttaacgtttt gttctctagt tctgccattg
     8941 tcaaataatc gctcattcag ctcccacttc ctctatcatt tcaattgatg cccctaaggc
     9001 tgttagtttc tcgataatat gatcataacc gcgtaagata taatctacat tgtaaatcgt
     9061 cgttgtccct tctgccaata gtccagcaat cactaaacaa gcacctgccc gaagatcaga
     9121 agccacgact tctgtgccgt gaagtttatt tggaccattc acgatgatca tatttccttc
     9181 cacggtaata tcagcgccca ttcgtgctag ttccggcaca tggttcacac gtttcgcata
     9241 gatcgtatcg atgatttctg aagtgcctat agtcatcaat agtaacgcag taagtggctg
     9301 ctgcagatct gtggcaaatc ctggataagg ataggtcatg atgtttgcgg gttttaattc
     9361 tttcgagggg taaacttcga tttcatcttc gctgattttc atatggacac caatttcctg
     9421 caattttgct ataaagcttt ccaaatgttc aaagatcaca tttttcactt tgataccatt
     9481 tcctactgca gctgctaatg ctagataggt tcctgcttcg atgcggtcag gaatcatgaa
     9541 atgccgacag ccatgtaatg tttcgactcc ttcgatacgg atcacatctg tacccgctcc
     9601 tcgaactttt gcccccatat tattcagtaa agttgccaca tctatgattt ccggttctcg
     9661 tgcagcgttt tcaattactg tctgtccttt tgcttttaca gcagctaaca tcacattgat
     9721 cgttgctccg actgaaacca cgtccatgaa aatccgattg ccacgcaatc ctttattttc
     9781 tgtccgtaaa tacatggcac catgttcatt tgtcacttca gcacctagag cttcaaatcc
     9841 tttgacgtgt aggtcaatcg gtctagggcc taaataacag ccacctggca ggcctactac
     9901 cgcttcgcca aatttaccaa gcagtgttcc cataaaataa taagaagcac gcaaactatt
     9961 gatttttccg tgcggcatcg gaatcgatac gacttctcga ggatcgattt ctagtatgtt
    10021 gttggaaaaa tggacagttg cgcccatgat ctctaaaatt tcaattaatg aatgaacatc
    10081 ttctatgtcc ggcacacctt ccaagatcac tggggaatca gctaagattg cagctggaat
    10141 caatgcgact gcactatttt ttgcaccgct gatcgttact tcaccagcta aaggtcggtt
    10201 tcctttgatt acgatttttt tcatctgttt ccaccttgtt tattaatctt ttttcatgga
    10261 tatgcttgta ttttatcata gataaaaaaa agaaacgagc atggtctgcc gttttaagta
    10321 aaaaagaatg gagagcgact tcaaaagtcg ttctccattc ctatatatcg tatctgttat
    10381 gtggatgctt acgctttgtt agctgaaccg aaccagtcga tatgagattt agcatcttca
    10441 acgattgctg ctttacctgg agctaataat ttacgagggt cgaatccttt accttcttta
    10501 tctttaccag cttcgatata tttacgagta gctgcagcaa atgataattg gaattcagtg
    10561 ttaacattta cttttgagat acccattgag atagcttttt cgatttgatc ttgaggaata
    10621 cctgatccgc catgtaatac taaaggcatg tctggaccta cagcatcagc aattgcttgt
    10681 aagtggtcaa aagctaatcc tttccagttt tctggatatt gaccatggat gttaccgata
    10741 ccgcaagcta agtagtcgat acctgtagca accatttgtt tgcattcttc aatatcagct
    10801 aattcgccgt taccgatgat tccgtcttct tctccgccga ttgaaccaac ttcacattca
    10861 actgagatac cttttgcatg tgctttttca acaacgtctt tagctaattt caagttttct
    10921 tcaaatggta gatgtgaacc atcgaacatt actgaagtat agcctaattc gatacattcc
    10981 aaagcagctt cgtaatcacc atggtctaag tggatagcaa ctggaacagt gatgttcatt
    11041 gcttcaacta aatcagtgat taaatcttta cagattttgt aaccacccat gtatttagct
    11101 gcacccattg atgtttggat aagtactggt gcttgttttg cttcagccgc ttcaaggatt
    11161 gcttgagtcc attccaagtt gtttgtgttg aatccgccga cagcgtaacc gcctttacga
    11221 gcagctttta aaaattcagc tcctgatact actggcataa taaaattcct cctaagtttt
    11281 gtaaaactaa atttgttaag acaattctta accaacgacc ctattttacc aaacatttcg
    11341 gtagttttct actaaaatca atgaaaatga aaattttcaa gaagtttttt gcaaaaaata
    11401 aaatatccaa tagcgcttta atcgaaaaaa gtcttttgct ttctatcttt tttcctacaa
    11461 ataataacaa atcagaaaaa gagaaaagtg acaagaaaat tgcttatccc cttctcttgc
    11521 tacttttctc tttctatcaa gttcaaaaat tattttgttt cgtgatctgc taatgatgct
    11581 ccgacaaatg ctttgattaa tttttgcggg cgatttggac gagaaatcaa ttctgggtgg
    11641 aattggcatc ccacaaagaa ttttttctct ggcagctcta cgatttctac taaacggtta
    11701 tctggtgaga cacctgaaaa gactagccct tgttcttcaa acaattgacg atatttgtta
    11761 ttgaactcat agcggtgacg gtgacgttct tgcacaacat cggctccatc atatgcttca
    11821 gccgtcttcg ttcctttttt gattttacat gggtataatc caaggcgtaa agttccaccc
    11881 aaattctcga tgttttcctg atcagccatt aaatcaataa tattgtttgc tgtatctgga
    11941 ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt tacgtgcaaa ttcgacacaa
    12001 gccatctgca taccaagaca aatacctaaa aacggaacgt cattttcgcg agcataacga
    12061 atcgcttcga ttttcccttc tactccgcga tcaccaaatc caccaggaac caagattcca
    12121 tctgcgtctt tcaaacgttc ttctacattt tctctcgtta attcttgtgc tttgatccaa
    12181 tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt tcaatgcttc aactactgag
    12241 atatatgcat ctggtaactc gacatattta ccgacaagcg caatccgtac ttttttcttc
    12301 aagttcaaga cacgttcttc taattctttc cattctgtca tgtctgcttc tggtgtatct
    12361 agttttaaat ggtcgcaaac aatttgatcc attccttgtg cttgtaaaac taatgggatg
    12421 gagtaaagtg tatctacatc tggcgactcg ataactgctt ctggttcgac atcacaaaat
    12481 tgtgccaatt tgtttttcac gctttgagaa acaggttgct ctgtacgtac caccaaaata
    12541 ttcggttgga tacctaaacc acgtagctct tttacactgt gctgcgttgg tttagttttc
    12601 atttctccag cagctttcaa gtaaggaatc aatgttgtat ggatatacat aacattatct
    12661 gtaccgacat ctgctttcat ttgacgaagt gcttcaagga atggcaaaga ttctatatcc
    12721 cctactgttc cccctacttc tgtaatgatc acatctgaat cagtcatttt agctgctcgc
    12781 atgatttttt ctttgatctc atttgtaata tgaggaataa cttgaacagt tgctcccaag
    12841 tattcgccct tacgttcttt acgcaaaact tcagaataga tttttcctgt cgtcacgttg
    12901 gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt ggcccagatc caaatctgtt
    12961 tcagcaccat catctgttac gaatacttct ccgtgctgat atgggctcat cgtccccgga
    13021 tccacgttga tgtatggatc aaatttttgg atagttactt ttaagccgcg gtttttcaat
    13081 aagcgtccta aagaagctgc aacaatccct tttccgattg atgaaacgac accgccggtc
    13141 acaaaaatat attttgtcat aagttcaaaa actccttttt aatttatgtg caggaaaagt
    13201 ctcagacaat aaacaaagct ccctattctt caataagaat agggagccga aatttctgta
    13261 aacttctgac cctctaaaag ggtgcccaag gtgtataata caagctgact aagggaaagt
    13321 caagtactca aatgaatact atccgcttac aattttttct ttttcgatat cctttgagaa
    13381 attatttctt aagtccttta tgagtgcaaa aaaaatttaa cccacataat aagcttgata
    13441 taaacatagc taacgttaaa cctaagcaaa tcagaaaatc gttactgaaa aattttaagt
    13501 gaaataatag ataaaaaaat aaaacaaaca ttatgataga taatatccca cctaacaaag
    13561 aactactttt tcctacttgc agcaacagtt tatatagctt atcgtagaaa caatcttttt
    13621 ctttcaccct tatttacctc ctttacgtaa aaaacgtagt ccacagttct cggatttttg
    13681 gcatatcttt tctattaaat aatatcaaaa acaaaacaat tccactaatt ttttcaaaga
    13741 tgaccttttt ttcaacaaaa gtgccttgta gggattcatt gaaggtaacg aatcacataa
    13801 gtaaaaaata aggagatgcg acaaaagtcg tgcctcctta tttctaaata aacggtgttc
    13861 tcctgccaac tcctcggtca caagtcacaa tatccatcaa acatgagaag ctgtttttga
    13921 aattatttct tgtgattcgg agttaaatgg taggctcaca acctctggat aaacggtatt
    13981 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
    14041 caaaattttt cttaatactc gaaactaaac gtctttttca aaacctcttg gtaaacggtg
    14101 ttcaaaagct tgcatctgcg gtaataagtt cagccaattc aaaaacaaga aaagctgttt
    14161 ttgcttggct attcttatta cttgttgcag ggcagctttt tcacaacctc tccactaaac
    14221 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    14281 ttttccgtta gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac
    14341 ggtattcctg ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg
    14401 tctgaatgcc gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc
    14461 taatgcgtca atcattacat tatccttacg caattctttg atagcattat ggatagttga
    14521 aggtaagtcc tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg
    14581 gtcaacagct gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa
    14641 tactgccatt gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt
    14701 ggataaaccg cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc
    14761 gacataaaca ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt
    14821 gcaaacggct gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt
    14881 ttcacttagt tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa
    14941 agacatattg caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt
    15001 cgcgtgaagt ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt
    15061 atcacaagca tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac
    15121 ttcatgatgt gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg
    15181 gcggcaattt tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa
    15241 ggtcgttgtg atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc
    15301 taaattgaaa gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc
    15361 gcgaggatcg ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc
    15421 aacttttcca tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa
    15481 atacatatcg ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat
    15541 tttattttct aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc
    15601 taagatgtct gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt
    15661 gatttcttca cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat
    15721 tcagtcaaag aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc
    15781 accttgcgcc aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt
    15841 cttgtttttt gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc
    15901 catattcaat ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag
    15961 cgaatacata cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata
    16021 atagcgaatt tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac
    16081 ggacatagat cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt
    16141 ggctaaagtg taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat
    16201 gagatctcta ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg
    16261 attttcgtac cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt
    16321 tatt
//
LOCUS       DACGLH010000061        16011 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.065, whole genome shotgun sequence.
ACCESSION   DACGLH010000061 DACGLH010000000
VERSION     DACGLH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.065"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            226..894
                     /locus_tag="IXT21_002079"
     CDS             226..894
                     /locus_tag="IXT21_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ4869463.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IXT21_002080"
     CDS             complement(1094..1480)
                     /locus_tag="IXT21_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869464.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IXT21_002081"
     CDS             complement(1452..2834)
                     /locus_tag="IXT21_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ4869465.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IXT21_002082"
     CDS             complement(2990..4177)
                     /locus_tag="IXT21_002082"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ4869466.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IXT21_002083"
     CDS             complement(4184..5215)
                     /locus_tag="IXT21_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ4869467.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IXT21_002084"
     CDS             complement(5231..6685)
                     /locus_tag="IXT21_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4869468.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6860..7765
                     /locus_tag="IXT21_002085"
     CDS             6860..7765
                     /locus_tag="IXT21_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4869469.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            7856..8533
                     /locus_tag="IXT21_002086"
     CDS             7856..8533
                     /locus_tag="IXT21_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ4869470.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IXT21_002087"
     CDS             8814..9710
                     /locus_tag="IXT21_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4869471.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IXT21_002088"
     CDS             complement(9765..11135)
                     /locus_tag="IXT21_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4869472.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IXT21_002089"
     CDS             11373..12119
                     /locus_tag="IXT21_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869473.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IXT21_002090"
     CDS             12116..12955
                     /locus_tag="IXT21_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ4869474.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IXT21_002091"
     CDS             12956..13906
                     /locus_tag="IXT21_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ4869475.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IXT21_002092"
     CDS             14047..14799
                     /locus_tag="IXT21_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869476.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IXT21_002093"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IXT21_002093"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ4869477.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5195 a         2999 c         3223 g         4594 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggatctactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctgcattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacaaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccacagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg t
//
LOCUS       DACGLH010000062        15962 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.066, whole genome shotgun sequence.
ACCESSION   DACGLH010000062 DACGLH010000000
VERSION     DACGLH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.066"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..450)
                     /locus_tag="IXT21_002094"
     CDS             complement(<1..450)
                     /locus_tag="IXT21_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4869478.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IXT21_002095"
     CDS             674..1465
                     /locus_tag="IXT21_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4869479.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IXT21_002096"
     CDS             1431..2384
                     /locus_tag="IXT21_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ4869480.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IXT21_002097"
     CDS             complement(2782..3756)
                     /locus_tag="IXT21_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4869481.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IXT21_002098"
     CDS             3871..4305
                     /locus_tag="IXT21_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4869482.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IXT21_002099"
     CDS             complement(4430..5416)
                     /locus_tag="IXT21_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAQ4869483.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..6266
                     /gene="arsC"
                     /locus_tag="IXT21_002100"
     CDS             5844..6266
                     /gene="arsC"
                     /locus_tag="IXT21_002100"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ4869484.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6546..8468)
                     /locus_tag="IXT21_002101"
     CDS             complement(6546..8468)
                     /locus_tag="IXT21_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ4869485.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8555..10495)
                     /locus_tag="IXT21_002102"
     CDS             complement(8555..10495)
                     /locus_tag="IXT21_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4869486.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IGVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(10783..11523)
                     /locus_tag="IXT21_002103"
     CDS             complement(10783..11523)
                     /locus_tag="IXT21_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ4869487.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(11536..12627)
                     /locus_tag="IXT21_002104"
     CDS             complement(11536..12627)
                     /locus_tag="IXT21_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ4869488.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(12617..13723)
                     /locus_tag="IXT21_002105"
     CDS             complement(12617..13723)
                     /locus_tag="IXT21_002105"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ4869489.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKANSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(13742..14419)
                     /locus_tag="IXT21_002106"
     CDS             complement(13742..14419)
                     /locus_tag="IXT21_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ4869490.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AILPAVN"
     gene            complement(14430..15209)
                     /locus_tag="IXT21_002107"
     CDS             complement(14430..15209)
                     /locus_tag="IXT21_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ4869491.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(15238..15588)
                     /locus_tag="IXT21_002108"
     CDS             complement(15238..15588)
                     /locus_tag="IXT21_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ4869492.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(15604..15960)
                     /locus_tag="IXT21_002109"
     CDS             complement(15604..15960)
                     /locus_tag="IXT21_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869493.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         4737 a         3345 c         2686 g         5194 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggcat cgatatttcc aaacagacgt ctgatttgat tgatagagat tactttactc
     6061 aggcagactt gatcattaca ctttgtggtg atgccaaaga caagtgccct gccgttccaa
     6121 acaatcacga acaccttcac tgggacctaa aagatcctgc acaagctgtc gggagcgaag
     6181 aagaaatatt aacggaattc agaaaagtca gagaaggaat caaaagaaaa gttttagact
     6241 taaatcaacg tacatcgatc gtataaagaa gtattcaaaa tgatttttca agcactaaga
     6301 aacaatgtta aaaaaatagc tttccatatt ttacaaaatt gtgccttatt gccgacaaat
     6361 tgtctcttga acaccgttta ccagtagtca agacatcgta agcaactata aaaaattata
     6421 agaaactcaa aataaaagga tgtgccacaa ctttggccac atcctctttt tttgtattta
     6481 gttttctact tccaaataaa tagatagaat tattataaat ataaaggcag ttttttaatc
     6541 gctacttatt cataactaga taacaacgtt aagatttcta gcggatgatt ggattgcatc
     6601 aactgagcca tttgttctgg ctgcttttct agatagtgaa taacagaaaa tatttttttg
     6661 aactctgcat gtttcaaatc atttttcttt attgctaata gcagtacgat acgtatctgt
     6721 tccccgtgcc ataggactgg ttctttcatt gtagcaatag aaatcatcga atgcttcaca
     6781 tacagcggat taccgtgagg gattgttaca aatggtgcta ctctagtact ggagtgactg
     6841 tctctttcca agacagaatc gatgaattcc ggctccacat aatgatgttc cactaagacg
     6901 tttcccataa agtttaaaat atcggctggg tctgtccgtt caagttgagg aaagatcaga
     6961 aatggatgag taaagcttgc tagatcaaat gtttttttct tcttgatagg cacatgctcc
     7021 agcatatatt ttgtcaaata ggctagatca gtttctgtga tcattggtga gatctcgatt
     7081 actggaatgc ttatatgttt aaatggtaac gtagccaaga taaaatcaac agagggaaaa
     7141 tcttgtctct ttaattctgg cttatattca actagatcca taatcgttaa ttctggaagt
     7201 tctctgttca gccgtgcttc gagaaaagta gccatagctt ttgaatattc actaactaaa
     7261 accgtttgat atcttctact tttctgatgt ttgtgtcgct cgatcgctgc ttggaaatgg
     7321 atcgttaaat aagctgtctc ctcttgtgga acataaaaat tttccttttc aaaataatct
     7381 tctaaaatta actgtaaaac taagaaaagt tgtgtgtacg tactaaacac atttttcgtc
     7441 aatggattag aaatctgcaa accataatgg agtcgaaaat aagttgaaga taaatgagac
     7501 cttaaatttt gttttagtag ctgatcttct tttaatgaat agcctagaaa ctgttccact
     7561 tgttcgatca aatgatcagc aagtgcgcta gattcctttt gaaaacgtgt ggctgcttgt
     7621 gaatttgtaa aaagattggc gatacgcaaa gccagataat tgatttcatc aggagaaaat
     7681 tcaattggat agatggtggc tagttttgct aatatttgac tacttaactt ttgtgcttgg
     7741 gtgtgttcca cgacttcgtt ttcgtcttta cttaatttca ctttctggta ctgtttcatt
     7801 cgctcgagca taaagtagat gtgaatctcc acactagcca gtccattcgg ctgttctaaa
     7861 tggttttcat aaaaaacatc ttgtaatgta tgttggataa cttttaatgt atctttagcg
     7921 aagaattctt tcaacgacgg actttgtgga tcttctttct gcatatttcg taatgtctta
     7981 acaagcaact gtcttttttc ttgttcatcg cctttaagaa aagttcctag ttttgttttg
     8041 gatactagct gtaaatggtg atactcaaaa aagctttgca actctgtcaa ttctttacga
     8101 atcgtacgtt ttccaacata tagtttttct gaaagttctt caagactaaa tccgtctttt
     8161 gctactagca gatggaaaat caataactga cgaagctgtt ttttatccat ttctgtttga
     8221 gtctcttcat taagcgctcg tttcagttgt tccctttcgt gaggcagacc attcaacaag
     8281 atacctgtac ctttttttct ttcaaacatt aaaggcagat gtttgtctct caaccaagtt
     8341 tcaatggtat tcaaatcgct atgaatcgtt ctttctgaca catggaaatg ctctgctagc
     8401 ttttttgttg ttatataatt attgttctca cttaattgat agatcaaaga ttgctgacgc
     8461 tgattcataa tgtgcagaaa ctcccttcta aataaaaaat cgatcatgat aacacatcta
     8521 ttatgtctca tcacggtcga tttttcatct tatgttacat ttcgcttaat aattggtgaa
     8581 ctgttgtttt atctggcgct tccatcagtg cttgacggaa gtcatcatcc atcaatttac
     8641 gtgatagacg ttgcagtatt ttcaagtgca tatcaccttg gctgttttca ggtacagcaa
     8701 tcataaagat catatgtgca ggttttccat ctaaagaatg ccaatctacc ccagattgac
     8761 ttctggcaaa tgccactgtt ggtttaagaa cataagctga ttttccatgt ggaatagcaa
     8821 tccccatacc gattcccgtt gtactttcat gttctctatc atagatgact tctttaaatc
     8881 tggttttatc tgtgatcgcc gctgtttttt ctaaacgttg gactaaatct tcgattactc
     8941 cgtcttttgt tgttgctgtt tcacctaaaa cgattgcttc atctgacata atatccgtga
     9001 tagaatcaaa agtttcagtt ttcgtataaa catcacttgt ttcagtctct gatagatctg
     9061 ctactgtcac accatttgct gcagcaaccg cagtttgttg taatgtcggt ttaaggattc
     9121 ttaacattag cacggttaca ccaataccag caatcactgc tacaaaaaac atggctacgt
     9181 gatccactgc tcctaaaacc gctacgatcg gaccgccatg agctacatga tctgtcactc
     9241 ctgaaagcat cgcgatcaca cttcctgtca ttgccccgat tacaatgctt ggaatcaccc
     9301 gtagtggatc tgttgaagca aacggaatag cgccttctgt aatcccaaac agtcccattg
     9361 ctaaggacgc cttaccagct tctcgttctg ttttcgtaaa tttggttttt aaaatcaatg
     9421 atgccagccc caatgctaat ggagggatac agatagctac agcaattggc cccataatgg
     9481 aataattgcc ttctgcaatc aagccagatc cgaacaaaaa ggctacttta ttgaacggcc
     9541 cccccatatc aaaagcaatc atcgctccta agatcatagc tagaacaatt tcacttcctc
     9601 cttgcatccc tgctaaaaag tgagtcaatg aactaaatag aagagctact ggttctccaa
     9661 taacatagat aaacacaaaa ccaactgcca atgatcctaa aattgggata acaataattg
     9721 gcatgatcga ttgtaaagct ttcggtactc gcactttctt gattcctaaa gcaatgaatc
     9781 ctgctaaaaa tccagtgatg atcccgccta agaatcccgc attcgcatca ctaccatata
     9841 atgctccgtt tgccgctaac atcccaccaa caaatccagg aacaagtcct ggacgatctg
     9901 caatactttg cgcaataaat gcagatagaa ttggaaccat caaccccata gcgatacttc
     9961 cgatactatt catcgttgcc caaatcgttc cttctggtat cttgatccct tcaggtgtag
    10021 ctgttcctcc taatgttaaa gaaatcgcga tcaataaccc tcctgtaact acgaaaggta
    10081 ccatgtacga aacgccattc atcaaactct tatagatttt tttacctaaa ttttctgatt
    10141 cacttttatt ttcggaagaa ataattgcct ccgtactttt atagactggc gcttttcctg
    10201 ctaaaatatc tctcaccaac tcatctgcat ggtggattcc ttcttgcaca cctacggtta
    10261 tcaaaggttt accgccgaat cgtgatttat ctattttagt atctgctgcg atgattacac
    10321 cagctgcttc ttctatatct cttgcagtca attcattttc aataccgatg gaaccatgtg
    10381 tttcgacttt tacctcggcg ccaactgctt tccccgcttt ctctaagttt tctgctgcca
    10441 tgtatgtatg ggcaatacca actggacagg atgtgattgc aataattttt ttcattttcc
    10501 taccgccttt ctacatttat agtatataaa aacggtttct ttttattaag cacaaaatga
    10561 ttccgtaata actgaaattc ttggaataaa tagaaatttc gctaaaaaaa ttgatatacg
    10621 atttattatg ttgaataaaa taatattgga aaatcttaca tttctgtact tctgcttacg
    10681 tacatctaaa ttattatcta cattaacaat ttccgtaatt aattataaaa agcttattcc
    10741 atttaatttc attctggaat aagcttctta ttttctaatg agttagtgaa taattttttt
    10801 taccatgctt aatagtatct ttacgtctcc tatacgcgta tctcctgttt ctttatctat
    10861 aattgaacta taaacatgag gaatcacttt ttttacacct gcatcgattg caattttcag
    10921 aatttcttca aagtttgaaa gatcaattcc tcctgttggc tctaaataga attcatattt
    10981 tgcgcaagct tccgcaactg ctttaaattc ttccctatgt gctaagcctt tcatcgggaa
    11041 atacttgatt gaagtacctc ccatgtcttg cagcatttga atggcagttt caataggcac
    11101 ttcagctgct ggcgcttgtg aacttaacgg acctgtagca atattgacat agccgatctt
    11161 acctgttggt gaaaccaatc cattgatgac tgtatcattt tggcccaata aagcacgaga
    11221 agtccccacg ccagtaaata cttgattgac atgttgtggt tgtaaaaccc cagataaacg
    11281 cgaaaccatc tgactttggt ttggatctcc agcgcctaat ccaactgaca acgcattatt
    11341 ggttgcgtct tggtaccttt tcatatcttc gattgctgct tcatcgctag cataattttt
    11401 cgatagcaca cctagtacga catgcccttc cgctgcttca tagcatgctt ttgcattttc
    11461 aactgaatga gctaatacat ttaaacatac acgattttta taataatttg ggataactga
    11521 caatttaaaa gcctcctaca tgatttctct caatcttttt ataatttttt ccatttcgtt
    11581 ttggctgacg gaacgaatgt cgaactcaat gattccgtta tttgcttgat attctcgggt
    11641 gtagactgct ggatttcgtg cttttaattc ctgaatgact tctttcgcac ttttatcgcc
    11701 attaatcgtt acacttgctc gataaatatc tctgccggca ccatcttgaa ctattttcgc
    11761 tgaaaggtca ggaagttgat tgattgcgtt aacaaaaggc gctaatcgtt gcttcatgga
    11821 gtcaccggat tcgctcccat tttttaaata atcttctagt gcctgggtaa agcctaaaat
    11881 attatcttta ccgattttca ttgctcggcc aataccttta gactgtagcc gaatccattg
    11941 aatatacttc ttttttccta tgaccagtcc ggcactcgga ccttcgattg cttttgcccc
    12001 agagtaaatc actaagtcaa cgcccatttc gctatatttg aataaatctt cttccgcagc
    12061 tgcatcaacg atcaatggca aacgatgagc tttggcaact tctaccattt ctgccaccgt
    12121 cagcatactt ttctgaactg catgatgact tttaatatat agaagcgcag cagttttttc
    12181 cgtaatcatc atggaaacat gttccggtgt acacatattt gcatagccag cttctatgac
    12241 ttttcctcca ccttgagcaa ccattacttc aaccggtgtc ccataatcta cattatgtcc
    12301 cttaggtatg atgatttccc gtctagcgat tttttctgta tagggatgat agacatggta
    12361 catgtcgccc tgtccgatta aagcagcaac agattgagca atccctgctg aagcagaaga
    12421 aacgatttgc gcatcttcca ctttcagtaa ttgagaaaga taagcgccgg ttttgatggc
    12481 aagatcactc atctcaaaaa aatgctcgcc tccaaatcgt tgtgctgcta ggacactttc
    12541 agatacctta gaaaccccta ggatcgtcat ttttcccgat gcattaatga cttcttttaa
    12601 atcaaacttt ccatagctaa ttgtcatata tgacgcctcc aataattgtt ttaatcggta
    12661 aaatttgttc tttagctata cgtgtaaatc cgttggaatc tttcaattcc ttttcatttt
    12721 caacaatctc aaatatcgtc aaatctgcat cataattgac agctaactgg cccttattct
    12781 tcaaatgaaa attccgtgca ggaactgctg taactttttc aatgatttct tcccaagaat
    12841 agccgactac cctcaatttt tctaaagtag ttgctagatc aaaaactggg ccatttgtac
    12901 gattgcggat gtagatatcc gtactaatag agttagcttt cattcctgct tttagtgcgg
    12961 tttctgccac atggaaatta aagctatccg tcccatgtcc aatatcaaag acgacaccat
    13021 tattgtaagc atcccagaca aagtttttaa tttgatctgt ctttggatct aaaataccgt
    13081 ttggttttcc attaaaacaa tgggtcaata catcgcctct ttccattctt gacaatactt
    13141 cttctaactc aggaggtgca gaaccgatat gaaccatcaa tggcagatca ttgttttctg
    13201 tctggatttt tttagctaat tctaatggct taatgccatt ttcaccaaca actgtcttac
    13261 tcattctcgc tttaatcccg acaataaatt ccggcagttc tgctaacgca gtacttacta
    13321 gatcttcttg aatctttgtt agatcagcaa gttcatcttg tttgacaatg ccccatttag
    13381 agatattcat tagcgcatac acatttgtct tgaccgattt cgtcaattga taaaattcac
    13441 ggatgttttc tgctcccgta gttccagcat caatgaccgt cgtcacacct ttttcgaccc
    13501 ctatctgatc aggatagtca taataaagat tcatcttttc atagcagtgg acatgatcat
    13561 cgatccaccc agcggataaa taatactttc cctccaagtc aactacttct tgcgccggct
    13621 gatttatttc tgctgcaact gctttaatca tgcctttttc tatagcgacc tctattttag
    13681 atccatcaac taaccttcca tttttaatca aagtatcaaa catttaatga ccctctttct
    13741 attaatttac tgccggtaag attgctccta aaatcattgc tcctaaaata gcacctccag
    13801 caattggttt tttccaagca tagaatccta gtgctcctaa tgtagcgcca atgccgattg
    13861 gaatagatgc tgcacacgcg cttaatataa ttaatggacc caaaaaacgt ccagatgaat
    13921 ttcctgcccc catcatgacg tcagcaccaa aagtggaatt tgattgattg atcgtaaatt
    13981 tacgaatcaa gacaatgatg ccaccaacaa tcaaacctag aaccaaacca gtcaataaag
    14041 agagtgcaaa attatcgatt ggtgccgcta ttttcatact taagagaatt gctggtaccc
    14101 caattccgat cccagttagg atcgaaccgc ctaagtccaa gatcccaact aaagagcctt
    14161 ctaaaattct ggcaaataag aaactagcac caaacgctgc cgctgcgcca taggatccgc
    14221 catccagccc ggcttttaac atcgccacga tcgaaacttc attgaatgca cccacaccat
    14281 acatcacata catgtgtgtt cctgcaaata cagcagcgga cataattcct acgattagcg
    14341 gaaaagacca gtctgcaaac caaaagctct tttgccgctt tgcttctaat acttttgctt
    14401 gttcttctgt tgtctccatg attctcctcc tatttcacac taaagatgtt gtgcaagtta
    14461 tcaagccaat taggcacttg aagctggaag ctcttgatca tttccatatc aaatccacgg
    14521 aaaaatccac tgaaaataaa taataaaaca atagcgatca tcatgctctt cgtgacgcga
    14581 ttccaaccgc tctcatcgac gcctttacca atcaagattc ctagaacaac accaggtacc
    14641 gcatttccca taatcaattg cgcgatacca ccaaaaagag tgccccaaaa accagttcgt
    14701 ttccctgcgt ctaaagctgc caaccaaaaa ataactggca ttactgtatt gatcagaatc
    14761 gtcgctgctg gtaccaaaac ggaaaccgcc gtcacttgta acgcctctgg aattgctgca
    14821 gctgttgtgt ttaagaaggc taccaccact gccccgataa ccgctccaat gatccccatt
    14881 tttttcggat catgcatggt ctgggtgaca tctttgtttt ttgctagaag tgctgccgct
    14941 gcccaattgg gaatgattcg atgcgtcaca tcttgtgtga atgcaccagc accaactgaa
    15001 gaagcccatg cattaaagaa aaagcctaaa ccaaatgaaa aatgagaagc tggatcaccc
    15061 atgcaggcat tcatttcgcc taatgttcta aaagctccta aaccttgtgt attaggtgca
    15121 tgaaacattc gagctgcccc gacagatgct gcaaaaccaa gtaatccccc gataatgacc
    15181 gatttgacta gaatgattac gaaatccacg ttattctttc ccccttatga ttcaaaatta
    15241 gattttttta gttacaaatg gaatggcaat cccatttgga tcttcagtca tttgctggct
    15301 gaacgatatg tctgccaatt ttatttctgt tacagccaca gtaacagaga gttcgacttc
    15361 atagctaata cgttgccgtg ggaagaagaa aaatagaaag cgttctgtgt atttgttttc
    15421 gaccgccttt aaaatatcca cattgactgg ttcgattcgc aataatacgt ttgattgttc
    15481 cattattttg ttttgaattt ctgctaacgc tgatgcaaaa gcatgttgct ttgtttcccc
    15541 tttaccggag acgataactt tttgctgaat tgtcttttga acgctcattt catgtccctc
    15601 tttctatttt ccatgctttt tgataaattc ttctgtcaga cgtttcccta attcctcttg
    15661 atccataaag ccgaaaccta gaacagtttt gccatccttg atagctgtga tcccttcttc
    15721 aatcgaacgc ataccatgtc gttctggata gccatatttt gttgctgctg ttaatgcacc
    15781 tgcaccacca ctaccacaaa aagagatacc catatctgca tgctcttgtt gcatcacatc
    15841 gcccaaacgc atatctgccc ccattcctgg tacgacaact gcttttccac cagctgcttc
    15901 tacccctttg gccacatttt gtcctttacc catacggtct gcaataacta ctttaatcat
    15961 ga
//
LOCUS       DACGLH010000063        15913 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.067, whole genome shotgun sequence.
ACCESSION   DACGLH010000063 DACGLH010000000
VERSION     DACGLH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.067"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(148..450)
                     /locus_tag="IXT21_002110"
     CDS             complement(148..450)
                     /locus_tag="IXT21_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869494.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(670..1036)
                     /gene="ssrA"
                     /locus_tag="IXT21_002111"
     tmRNA           complement(670..1036)
                     /gene="ssrA"
                     /locus_tag="IXT21_002111"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1207..1980)
                     /locus_tag="IXT21_002112"
     CDS             complement(1207..1980)
                     /locus_tag="IXT21_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4869495.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2124..2474
                     /locus_tag="IXT21_002113"
     CDS             2124..2474
                     /locus_tag="IXT21_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ4869496.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2455..3171
                     /locus_tag="IXT21_002114"
     CDS             2455..3171
                     /locus_tag="IXT21_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4869497.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3171..4619
                     /locus_tag="IXT21_002115"
     CDS             3171..4619
                     /locus_tag="IXT21_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4869498.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4690..5199)
                     /locus_tag="IXT21_002116"
     CDS             complement(4690..5199)
                     /locus_tag="IXT21_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869499.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5189..5914)
                     /locus_tag="IXT21_002117"
     CDS             complement(5189..5914)
                     /locus_tag="IXT21_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869500.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6137..8257)
                     /locus_tag="IXT21_002118"
     CDS             complement(6137..8257)
                     /locus_tag="IXT21_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ4869501.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8487..9665
                     /locus_tag="IXT21_002119"
     CDS             8487..9665
                     /locus_tag="IXT21_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ4869502.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9681..10439
                     /gene="aroD"
                     /locus_tag="IXT21_002120"
     CDS             9681..10439
                     /gene="aroD"
                     /locus_tag="IXT21_002120"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ4869503.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10462..10947
                     /gene="ybaK"
                     /locus_tag="IXT21_002121"
     CDS             10462..10947
                     /gene="ybaK"
                     /locus_tag="IXT21_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ4869504.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11018..12271)
                     /locus_tag="IXT21_002122"
     CDS             complement(11018..12271)
                     /locus_tag="IXT21_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4869505.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12388..13737
                     /gene="gdhA"
                     /locus_tag="IXT21_002123"
     CDS             12388..13737
                     /gene="gdhA"
                     /locus_tag="IXT21_002123"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ4869506.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13849..15195
                     /locus_tag="IXT21_002124"
     CDS             13849..15195
                     /locus_tag="IXT21_002124"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ4869507.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15503..15889)
                     /locus_tag="IXT21_002125"
     CDS             complement(15503..15889)
                     /locus_tag="IXT21_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4869508.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5208 a         2843 c         3187 g         4675 t
ORIGIN      
        1 agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc atcatattac
       61 acaatcttaa tactaactta aaaaatatcc cttttataag tatggtgata aaatccgttc
      121 cgggctacct tttttaggta gcctacttta atctttatac ctttctggat caacgaaagt
      181 atacttcaaa tagtcataac gccgatgatc gctccgtgcg tctggcacat cagtcacgat
      241 atcaaacaaa aaatacacat ctttcttcat tctggttttt gttgccggaa ttttaaagta
      301 gttcttatta gagtaataga gattgattaa taagctatct tcgattgcta aaaagaaaac
      361 ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa
      421 ccataattgt gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact
      481 aatgttcttt ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa
      541 aagtgttctt tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg
      601 aaaataaaaa atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct
      661 gaaatctcac aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa
      721 aatctacgtt catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc
      781 attctgtatc gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta
      841 tcccactaaa gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta
      901 actgttttta ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact
      961 gtaacgtttt tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg
     1021 aatccgtaac gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta
     1081 tagcatatat tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata
     1141 actgcccctt tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa
     1201 aagatcttaa atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt
     1261 ctttgtgata aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt
     1321 gttgtcgcaa gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg
     1381 aactttttcc agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc
     1441 tttcttcact gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg
     1501 ttcgtgatag tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc
     1561 tttaaagcct aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat
     1621 cattgtttgg ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg
     1681 accgttcaac tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga
     1741 atagatttct tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat
     1801 gcccgcatct tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg
     1861 gagaacattg ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga
     1921 tttcgcatct agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac
     1981 aaataaaact ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa
     2041 acctttcgtg aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa
     2101 gaagagaatt taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa
     2161 ttggcagtct ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt
     2221 cgcctcttgc cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact
     2281 ggttttttct tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc
     2341 atacgaatta ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta
     2401 ttatgacaga aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat
     2461 attttaatga ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat
     2521 gaaggttggg aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa
     2581 aacccacaac attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg
     2641 ctagtaggaa gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct
     2701 agagattctg aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca
     2761 aaacctttca gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag
     2821 ttggcagaag caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt
     2881 catttcaaga tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga
     2941 ctgacgccta aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc
     3001 tctcgggagc agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca
     3061 gtggacgccc atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc
     3121 caaactgttt ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt
     3181 tgtatcagca actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag
     3241 tttcttttac acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg
     3301 gatatgctga atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta
     3361 gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa
     3421 atgtgaactt tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt
     3481 tactgaattt taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa
     3541 aattcacatc gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc
     3601 catttaattt gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata
     3661 tcgataacag cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta
     3721 tcgttgcagt tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt
     3781 taaggaatgc gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg
     3841 acaaagatga atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag
     3901 aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt
     3961 ctcacgagat gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca
     4021 atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc
     4081 gtctgatccg tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca
     4141 gtatggtcat taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag
     4201 aagctaaagc agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg
     4261 tttacgctga ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac
     4321 agtttactga aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca
     4381 tcacgattga agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc
     4441 gttattataa agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt
     4501 taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac
     4561 taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa
     4621 aaacaagcgg tataaaagat catgaatcga tcttctatac cgcttgtttt ttactataaa
     4681 atgattgatt tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc
     4741 atcatgagta aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg
     4801 gagtagctct tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt
     4861 gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa
     4921 ctcagctgcc gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa
     4981 acgatcttgt ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag
     5041 ataaggaaac atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag
     5101 aacctctttt ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac
     5161 ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt
     5221 gcaaatgatg gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga
     5281 gcccaataag agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga
     5341 aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca
     5401 agatatcacg gatatctggc agttcagtag tctttaagac aagaaaattg atcctttgaa
     5461 gaagcgcttc ataaactgac tggaagctta cgatccacaa taaaaccaag atacaacaaa
     5521 gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa taatttggtt ttagaaagac
     5581 cagtcaggtg ccaattgatc aattcctgct tgttttgcct gactgaatag taaaagaaaa
     5641 agataaaaag gaaggcaaat agaaaaaata aactgaatat gccttttata taaaatcggc
     5701 tagctaattc taaagccgga agaagctctg tagatgcaga agcgccaata aatgaaagcc
     5761 gatcttggac ttgattggct aaagccgatt gcaaatggta aagagtcagg catgctatca
     5821 ataaaaaagc agaaaagctt gaaaataaac aatagagtat tgtctgcttt ttgtaataaa
     5881 ttaatcgagc agtagcatag cggattattt tcatactttt cacctctata aaaagtatag
     5941 aaaatgaata tgaataaagt atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa
     6001 atattttgta aaaagcaaga ggttgtgaag aagctgccct attctattac cgtagatgca
     6061 agcttttgaa cactgtttat tcgaaaagag gagcagcgac aataattttg tcgctgctcc
     6121 ttttaaattt attttatcat tcggaagaac ttgtggtcgt tccgtgaagt tcttggtacg
     6181 tttgcgtttt ataaagatca acagttgatt gtccgttgtg ttcactaaag acactagttg
     6241 acttgtcacc aagagatttt tcagcttctt ttaatttctc taagccattc ttgtagtcat
     6301 aatcactggt atcaattggg tctaatccac tattcgtata gaaccgaaga agatcaccat
     6361 tattgatttg gtcagaaaca gctaactgtt tatcaacttt ttctttcagt gaatcaattt
     6421 cattctgtac ttcctcagta ggttcagtaa tagcttcacc agtttcgtta ctatagatcg
     6481 taccaccgta gtaagtatat tttggtgtaa caaaatcacc atcacggaaa gaaacaagct
     6541 gttcatgatc tttcgagaat agatcttgtc ctaactgaat ataatttttt gtatctacac
     6601 ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc gccgtaagtg tgattgattc
     6661 cgccctgtgt ttgtccaggg atatggatca tataagggac acgctgcatt tgtgcattgt
     6721 cgtagtttgt ccaagtagaa cttgtttttc caactaattc agctagattt ttatttcttg
     6781 aattcgagac accgtaatgg tcaccgtaaa ggacgatgac tgaattatca tacaagccgc
     6841 tttctttcaa ataattgaag aattcttgta cggcggtatc taaataattg gctgtcgcaa
     6901 agtaaccgtt gatcgtttca tcatttgtat tggctaacgg gaatcctgct tcgtcattgg
     6961 tgaattttga ataaggataa tggttagata cagcaatgaa ttttgaataa aatggttgtt
     7021 gcagacgttc caagtattgt acggattgtt ggaaaaatgg tttatcatgc aacccgtatt
     7081 ggaacgaatt gtcatcatta acatcataat aagaagaatc aaagaaataa tcatatccta
     7141 aattcttgta tgtctcgttt cgattccaga atgtaccagc gttcccatgg aataccgcac
     7201 tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc ttggaaagta ttttttccgc
     7261 ctaattgtgt gaacaaggag ccttgattca agccaaacaa tgaattttcc atcagcgttt
     7321 ccgcatcgct tgtttttcct gcttttactt gatggaaaaa gttgtcaaaa ctgaacgtgc
     7381 tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc atgctggaga ccattctcat
     7441 ctgtcaattt ataatcgatc aggaattgct ggaagctttc taaatggata taaatcacat
     7501 tttttcctct agcgagcccg aacaaatcgt catttggtgc tgcataatgt tcttttacat
     7561 aattttctac ttctttcata tcattgggac ttgcttctgc ccgcacttgg tttgtctggt
     7621 aagtttgaac accgtcataa accatgaaag cattcaagcc aagatatttg accaaataat
     7681 cacgagagaa tgttcgagtc agcaattctg gacgatcagc ttccgccata aaaagatttc
     7741 ctgagaaaat cataactgct aatgtagaga tcgcaaaagc cattcttgct cggatcgggc
     7801 gagggtccaa cttgattttt tttgcaatca gcagaacagt caagataata aaatcgatcc
     7861 aatagatcac atcatatggg cgaaacagtc gaatggcact ttctcccaga ccactcgcta
     7921 ctttcccagc acctaaaatc gtgttgatcg taatgaagtc cgtgaattct cgataataag
     7981 tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat gaccatcatc gtgatataag
     8041 caactctagg gcgtctgata taaagagcaa ttccaagtaa taacaatgta gttgcaatag
     8101 ggttgataaa caagataaag tattgaatgc cactttcgat tccgagatgg aaatcaacta
     8161 aataggcaaa aatcgttttc aaccaaagta aaacgactag taaagaaaaa aatccgagcc
     8221 tcttattcaa aaaggcaggt gtttttatac atttcaagat aatcttcctt tctgcacttc
     8281 ttaatcacag aagtgtcctg aaaccatgtt acacagctca cttattctca tttattttac
     8341 tagtatgaca atatagtgtc aattgaaacc agaagtttat gctgtttcat taaggttttc
     8401 tttaagcttc atgaaaaaat gagcagtaag acaagtccta tgttttttgt tatacttggg
     8461 aagagaaagc taaagcaggt gaaggaatga aagtaaaagt aaaaaaaaga gcaagcaatc
     8521 gattgaaaaa aggatatcca ctgatccaaa aagaagacct tgctcatccc caaaaagaaa
     8581 ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt tatcgcaaaa ggatatctag
     8641 gaaagcagaa taagggaatc ggttgggtat tgagccagca gaacatccca tttgatcaat
     8701 ctttttttga agaatttttt gtgaagtcaa aagaaaaaag aactttcttt ttttcagatg
     8761 attctactac cgcttttcgt atattcaatg gagaaggaga cgggatcggc ggtctgattg
     8821 ttgattatta tgcaggttat gctgtttttt cttggtataa cgaaacgtta tatgcacatc
     8881 gtgaattatt gattcaagct tttcgtgaaa tctacccgga aatcatagga ggctatgaga
     8941 aaattcgatt tgaatcagta aatctgcctg agagccagtt catttacgga aaagaggcac
     9001 cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc cgtttattta aatgaaggat
     9061 taatgactgg gatcttttta gaccaaaaag aagtacgggg atcattagca gaaggtttcg
     9121 cttctgggaa aacagttttg aacatgttta gttataccgg cgccttttct gttgctgccg
     9181 caatgggagg ggcaatcgaa acaacaagtg tcgatctagc aaaacgcagt ttaaaaaaaa
     9241 cacaggaaca gtttgaagtg aatggtttgg atgtcgatca gcaaaaaatc attgttatgg
     9301 acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt tttatatgac atgattgtat
     9361 tggatccacc aagttttgcc agaaataaga aaaaagtctt tcgcgtagca aaagattatg
     9421 gggaactggt caaggatagt ctggatattt tagcagaaga cgggatactt attgcgtcta
     9481 caaatgctgc gaatgtctcg atagaacaat tccaaacaat gattgaagag gaattccatg
     9541 ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct gccaaaagat ttccagacga
     9601 cggatacctt tttagaaggc aattatctga aagtgtttgt ttatcaaatt aaaaataacc
     9661 attagaagga gcgatgcaga atggttcggg taaagggatt gaatatagga agcggtagac
     9721 caaagatttg tgcgcctctt atcggggccg tccgagagga agtattacgt gaagcagttc
     9781 ttgcacgtga agcaggggcc gatttagtcg aatggcgagt cgatcattat cgtgaagttt
     9841 tttcttttga tgaagtagca gaaacattgg ttcttatcca tgagatgctt gatggactgc
     9901 cgttattatt tacgtttcgt accttagctg aaggcggaga acaagacata tcgatccgtc
     9961 agtatcgcga cctttatgaa catgtgatac acacaggatt agtagatatg gtcgatatcg
    10021 agttgttcaa agtagaaagt ctaggaaaaa gactgataga ggaaatcaag tctttaaaca
    10081 ttcctttaat tatcagtagt catgatttta aagaaacacc agcagaccct gtcttgcttt
    10141 accgattgaa tgttatggaa cattttggag ccgatatcgg aaaactggca gtaactccca
    10201 ataatgaacg agatgttttg cggttgatgg agttgactag acgggcaaat gcttttgtct
    10261 ccatgcctat tattacgatg tccatgggaa atcttggtaa aatttctcgt ctggttggcg
    10321 gaacgacggg ttccgttatg acatttggtt ccttaagcag cgattctact tctgctccag
    10381 gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt attaaatgaa ccgttgtaga
    10441 acaagtttga ggtgatattt tttggcaaag aaaaaaatag taaaaacgaa tgcaatccgt
    10501 atggtcgaac aaaaaaagat tccctataca gagcacgaat atgagtggga cgaaagccac
    10561 ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc aatctcgtat atttaaaaca
    10621 ctggttgctg tgggcaatgt gacggggcct cttgttgctg tgataccggg agaagccgaa
    10681 ttgaatctaa aaaaattagc aaaagtttca gggaataaaa aagtagaaat gctccattta
    10741 aaagatttgg aagcaacgac cggatacata cgtggtggct gctcgcctat aggtatgaaa
    10801 aaactatttc ctacttattt agatcaaatt gcagaaagct atgaacaaat cattgtctcg
    10861 gctggcagaa gagggctaca gatggagtta gcacctcaag atatctgtgc attaacgagc
    10921 ggacaatttg cagatataaa acaataaatc atacgtataa aagaaagaag gaggccggga
    10981 tgttgtttgt ccagtctcct tctttctttt ttatacatta tgctctttgc ccggcattca
    11041 ttcctgctac atgccctgta gcgaaagccg ctgtgatatt aaagccgcca gtataaccat
    11101 tgacatccag taattctccc gcaaaataca gaccatcaat gattttactc tccaatgttt
    11161 taggataaat ttcctttaga gaaacccctc cgccagttac aaatgatttc tcaataggaa
    11221 aagttttgtt gattgatagc ttaaactctt tgcatagttg aacaaaatct tgaatttgtt
    11281 tttgagaggc ttgttgtcct gtcagatgat ccatttcaag ccgctcaaga aagaaaacta
    11341 acaatcgctc aggcaatata ccatgccaag cattgaccaa gttctttttg gttgctttac
    11401 ttttttctgt caatatatga ataagctctt gtttggtttg tgtagggaaa caatccagag
    11461 aaagtgtcac tggttctccc gttttctcta attctttatt gacaaaactt gaacaacgca
    11521 gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa atccatcgta tgttcagtca
    11581 atacacgacc ttttttattt aaaacgcgta aagtgatgtc ttgtaaagaa agcccttgaa
    11641 gtgttttctc ctgaatatac ggttcatccg atatcaaggg ggactctgtc gcataaagag
    11701 gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc atctccagta gaaccagtag
    11761 atggataagt ccgtccacca gttgttaaga taatggcagg agcatggaac tcttcgaatt
    11821 ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa tttctctact cgagtagaga
    11881 atagaagcgt cacatctagt tctttcagac gattgagcaa tgcttcgata atcgtttttg
    11941 atttatttgt tacaggaaac atccgtccat gatcctcttc ttttaaatgt acgccttgtg
    12001 attcaaagaa gttcatgata tcgaaattat tccattgcgc aaacgtacta tataaaaact
    12061 ttccatttcc aggaatatgt gcaatcaaat catcaacagg acgattgttc gtaacattgc
    12121 accgaccacc acctgtcatc agtagttttt ttcctgcttt tttgtttttt tcgatcaaga
    12181 gtacgcgtgc gccttgttca gctgcactga tcgctgccat catcccgctt gttccagcgc
    12241 cgactacgat cacatcatac agtttttcca ttccctcacc tcaaggaaag tgtagcatat
    12301 ctgaagaaag aaaacagaaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
    12361 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
    12421 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
    12481 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
    12541 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
    12601 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
    12661 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
    12721 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
    12781 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
    12841 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
    12901 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
    12961 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
    13021 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
    13081 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
    13141 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
    13201 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
    13261 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
    13321 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
    13381 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
    13441 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
    13501 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgtccagcga
    13561 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
    13621 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
    13681 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
    13741 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
    13801 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
    13861 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
    13921 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
    13981 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
    14041 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
    14101 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
    14161 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
    14221 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
    14281 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
    14341 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
    14401 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
    14461 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
    14521 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
    14581 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
    14641 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
    14701 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
    14761 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
    14821 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
    14881 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
    14941 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
    15001 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
    15061 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
    15121 aagaaaaaca tgttcgcact tctaggcaaa ccaggctttg aagatcttgc taaagaattg
    15181 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
    15241 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
    15301 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
    15361 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
    15421 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
    15481 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
    15541 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
    15601 agatcgttcc ttcggggtaa gcagaaccat tggcagagtc accagaagta atgacatcga
    15661 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
    15721 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
    15781 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
    15841 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
    15901 ttttttattt tta
//
LOCUS       DACGLH010000064        15515 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.068, whole genome shotgun sequence.
ACCESSION   DACGLH010000064 DACGLH010000000
VERSION     DACGLH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.068"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            46..327
                     /locus_tag="IXT21_002126"
     CDS             46..327
                     /locus_tag="IXT21_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869509.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            430..744
                     /locus_tag="IXT21_002127"
     CDS             430..744
                     /locus_tag="IXT21_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ4869510.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            722..2416
                     /locus_tag="IXT21_002128"
     CDS             722..2416
                     /locus_tag="IXT21_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ4869511.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2436..3614
                     /locus_tag="IXT21_002129"
     CDS             2436..3614
                     /locus_tag="IXT21_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ4869512.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            3577..4263
                     /locus_tag="IXT21_002130"
     CDS             3577..4263
                     /locus_tag="IXT21_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ4869513.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            4263..5423
                     /locus_tag="IXT21_002131"
     CDS             4263..5423
                     /locus_tag="IXT21_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ4869514.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            5433..6308
                     /locus_tag="IXT21_002132"
     CDS             5433..6308
                     /locus_tag="IXT21_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869515.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            6305..6616
                     /locus_tag="IXT21_002133"
     CDS             6305..6616
                     /locus_tag="IXT21_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869516.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            6606..6959
                     /locus_tag="IXT21_002134"
     CDS             6606..6959
                     /locus_tag="IXT21_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ4869517.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            6949..7350
                     /locus_tag="IXT21_002135"
     CDS             6949..7350
                     /locus_tag="IXT21_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869518.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            7343..7747
                     /locus_tag="IXT21_002136"
     CDS             7343..7747
                     /locus_tag="IXT21_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869519.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            7759..8367
                     /locus_tag="IXT21_002137"
     CDS             7759..8367
                     /locus_tag="IXT21_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4869520.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            8387..8749
                     /locus_tag="IXT21_002138"
     CDS             8387..8749
                     /locus_tag="IXT21_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869521.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            8752..8934
                     /locus_tag="IXT21_002139"
     CDS             8752..8934
                     /locus_tag="IXT21_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869522.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            8952..12284
                     /locus_tag="IXT21_002140"
     CDS             8952..12284
                     /locus_tag="IXT21_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4869523.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            12335..13072
                     /locus_tag="IXT21_002141"
     CDS             12335..13072
                     /locus_tag="IXT21_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4869524.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            13082..15373
                     /locus_tag="IXT21_002142"
     CDS             13082..15373
                     /locus_tag="IXT21_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869525.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
BASE COUNT         5501 a         2272 c         3274 g         4468 t
ORIGIN      
        1 tagaaagctt tgctaacttc gatgcaagtg aaaggtggta agtgcatgat catcaatgat
       61 aatggcagag agtatgatac agaaaagatt gaagagtatt catcttatac tcagggatta
      121 attaaacgtt tgatatacgt tcgctatgta ggtattaggg atctgttatc agataactgt
      181 tgtagtaaat acaaagtgaa tcaagtaaga gaagcgttga ataaagataa taacgtcgaa
      241 agaataaaaa atgtttttgg atatagtatt gaagagatta attattacat tgacttcgct
      301 gaagctttca ttccgatggt gagataaccc ccccttaaaa taaatcgcaa attttttggg
      361 ggtgatgaaa cggagggggc tgtcaggaaa agagattttt tcgaacttta tcatgaaagg
      421 agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct acagagaaaa attacgccgc
      481 tctttaataa gccaattgga aagtcagaaa acaaatattg agccattctt agataatgtt
      541 gatcgttata tcagtttatg ggaaacggcg atatcactgg aagaagatat atccgagaac
      601 ggcattagac tggagaatgg taaaaagaat gaatcagtag cgttgcttgt ttctgtcaac
      661 aaacaaatgg gattgatgtt ggataaactt gccattactc ctgaattggt aggtgaagca
      721 aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg ttcaaagcta tagaagaagg
      781 cactatcaag gtatgcaaag agcaattatt gctaaaaaat tatctagaag aaagagtctt
      841 tactagagaa gatatttact tcgataagca gatggtagag gattcaatca atataccagc
      901 acaatacttt ccattcgaat taattccgtg ggaaaaattt ctacaatgtt ttatttatgg
      961 tgttcgatgg aaaaaagata aaacactagt gttcaataga tatctttcat taatgggacg
     1021 tggtaatggt aaaactggtt ttgcttcttg gaacaacttc tttctactaa ccgctaaaca
     1081 cggtattaaa aattatgata ttgatatcta tgccaataat gaaagccaag caaagactag
     1141 ttttgatgat gtatttaaag taattaaaga tcatcctgat ttagataaaa aagtatttaa
     1201 agctacgaag gaagttattc aaaatatcgc tacaaacagc aaacttcgtt ataacacggc
     1261 aaatgctaga acaaaagatg ggaagcgacc aggtgcaaac cgctttgatg aaattcacga
     1321 aaatgaagat tattcaatga taaatgtggc tacttctggt ggtggtaaaa ttcgagatta
     1381 tagagaattt tatgatacaa ctaatggtca tgttcgtggt ggtccgcttg atgacattat
     1441 agaagaatca aaaatgattc tttctggaga acttggaatt gacaaggatg gagcagaatt
     1501 ttctagtttg tttccattta tttgtcgctt ggataacgat aatgaagttg atgatcccga
     1561 catgtgggaa aaagcttgtc caactattaa ttacaatgca gatttaaaac ggaaaatgtt
     1621 tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga cttacgttca tgaccaaacg
     1681 aatgaacaga cctatggaag atacacgatt tgctgttgct tcatatgatg atgttctgca
     1741 tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg ataggaacag tcgattttgc
     1801 tgatagacga gattttgcca gcgttgggtt gctaggaaaa tacgataaag atgtttattt
     1861 tacacaacat acttttatcc acgaatcagc ccttcgatta caaaacatca aacgagaggt
     1921 tatagatatt tctatagatc aaggaaaatc acagatcgtt catggaaaaa atatagaagc
     1981 tgattatatt gtaggttggt ttcttgaaat gagtaataaa tattatatta aaaaaatcgc
     2041 tatggatatg taccgtgcaa aaatattgaa gcccgcttta gaagaagcag gttttactgt
     2101 ggaaattgtt cgaagcggat ctgttacaca tggtatgtta aaagatctgg ttgatgacct
     2161 ttttattaat caacgtttat tttttggtga cgatgcgatt atgcgttggt attgcatgaa
     2221 tgtatatgaa gagcatattt ctaatggaaa tatacgctat gaaaaaatag aacctgaaac
     2281 tagaaaaacg gatggctttt tttcattcct tcatggtttg aattttttag atgatattta
     2341 tgattctgct cctgtaacag tcacaaatag ctcagtagaa aatacaggaa ctggatttac
     2401 tcctctagta ttctaacttg aaaggaggtg agaaagtggg gatttttcaa aaggcggtag
     2461 gatacttcac aaaaaaagca acggttcctt tagaagaata cttttgtaaa ttgcaagttg
     2521 attttgtgta tcgaaaattt gcgattgaaa cttgtattga tttgattgca aatgcgatga
     2581 gtaaagcgga attcaagtca tatgaagatg gaaaaaataa aaagaatgat ctttactata
     2641 ggctgaatgt agctcctaat aagaaaaata atgcaacaga atttagaaaa aaactgatca
     2701 ggagattaat attctacaat gaagtattga tcgtttctcc gtctaataat tctagcgaaa
     2761 tatttattgc ggatagttgg gatgtcacag aatatgcatt gaaagatgat gtgttttctc
     2821 aagtgcaaat taacaacata gtccttgata gagaatttct agaaagtgat gttatctata
     2881 taaaatacgc agatcaacaa attaggcaac tagtcgatgc gtattatcaa gcgtatggga
     2941 aactcatttc tagtgccatg aatgtttaca agcgctctaa cgctcgtaga tacgtactga
     3001 aagggaattt attccgaccg caagacaata caacacaaga tcaaatcaat aaaatgatga
     3061 catcacaatt taaggctttt atggaagctg ataatgcagg tgcggtattt caattacaaa
     3121 atgagtacac attagaagat ttcagcggaa acttccaaag caattcaaga gatataaaaa
     3181 acttaataga cgacatcttt gagatgacag cagcagcgtt tcacgttccg aaaaacctac
     3241 taaagggaga catgagtggg ttatcggatc aagtggacgc ttttttaatg ttcgaaatca
     3301 taccgattgc tgaacttatt caggatgcgt ttaacgctag tctctatgaa gtagaagaat
     3361 acttgtcagg gaattttgta cgtgtggata caactatgat caagattact agcttcaaag
     3421 atttggttga cgctattgat gtaggcatta gaaatggagt atttacaatc aacgaaggaa
     3481 gagaacgcgt tggaaatgat cgctctgata aggcgatggc agatgaaata tttataacta
     3541 aaaacaatca acaagtatcg aaaggaggtg aggcgaatga cgacaatgaa aacatttcta
     3601 gcagtaaaga atgaaggcgc agtaccgcaa atttttattc agggatttat tggttctagt
     3661 tggttctttg aagggaatac tgacaaggga atcaaaaata ttttggatag tctaggtgat
     3721 caagaagaaa ttgaagtagt aattaattca aacggtggag acgtatttca agggattgct
     3781 attgggaact tacttaagtc aaataaagca aaagttaacg ttgtgattaa cggcttagcc
     3841 gctagtgctg cttcaattat cgcaatggct ggcgatacta taaaaatcta caacaatgca
     3901 caattgatga ttcaccgcgc ttccacatac ggagaaggaa atgtcgatga cttccgtacg
     3961 attgctgacc aactggaatc aattgataaa tcggtaaagg cttcatataa aacacgattc
     4021 aatggcacag atgaagcatt gcaagaactt cttgaaaaag aatcgtttat ggatgcagaa
     4081 acagctttga gttatggatt ggtcgatgaa attatcgatg cagaaaatag ctcaggtact
     4141 gaagctaaaa aagaacaaag cgttgaagaa attttgaatg acgttaaaga aaaaagagca
     4201 gaaaaaattg ctgcatttac agcagcatta aataaaacat ttggacaagg agatgtaaaa
     4261 taatgacagt taaaaattta aaaggtgtaa cagctgcaag cgaccaattg atgaaagctt
     4321 ttaaagatgg taacgaagaa tcttttagcg cagctatggt aagcttatct aaggaaattc
     4381 aggataaaat tttagaagaa gcaacagcaa aaaatcaaga tcaattagta ttaatgaacc
     4441 gtggtcagcg tgtattaact acacaagaaa caaaattcta taacgaagta gtgaaaaacg
     4501 aaggttttgc aggggtcgaa gaattagtgc cagctactgt atttgaacgt gtatttgaag
     4561 atttagaaca atctcatcca ctattgcaaa aaattacttt tgttaacaca actggtgtaa
     4621 cagaatggat tgtgtcacgt ggagtcaatc cagcatggtg gggtaaactt tgcgaagctg
     4681 ttaaaaaagt tttagataat ggctttgatg taattaacat gaagcagttc aagctatcag
     4741 gttatattcc tgtatgtaag gcaatgcttg atttaggtcc agtatggtta gatcgttatg
     4801 tccgtactgt tttagtagaa tcattgagaa ttgcattaga acaagcaatt gttgatggta
     4861 ctggtaaaga tatgccagtc ggaatgatgc gtgacatgag caaacaaact agcggagaat
     4921 atgctgaaaa aaaagcagaa cctattacag ctttagatgc tgtaactatg ggcggtttga
     4981 tggcgcgact atcaaaattc aatatcgaag gtgtgaatga tccgatttat cgtaatgtga
     5041 atccttctga tgtggtccta attgtgaatc caactgatta ctggtctaaa gtattcccag
     5101 ctaagactgt actaactgct aatggagaat acgtacaagt attgccagta ccagtttcag
     5161 atttgcagtc aacggctgtg ccagaaggaa aagcagttat tggggtagcc tcagattact
     5221 tcatgggtgt aggatctaca ctaaaaattg aagcttcaga tgaataccat tttgttgaag
     5281 acgaacgcat ttatctagct aaacaatatg caaacggtca acctaaacgt aacgatagtt
     5341 tcattgtatt agatattagc gctttgggaa ctactactac aactacaaaa ccaacaacca
     5401 caacaactac aacacaagcg taggtgatca gaatgaagta tattctttgt cagccggcaa
     5461 tcaatcggtt taaatgggag cttgaagttt gtttaactaa tctgaagaaa ctaggaatca
     5521 aagatatcgt attgcttttc agcagacacg atgatcagat tcctattttt tttgagaagg
     5581 aatatggtgt tgaagttcat gtgtacgatg atctgcggga cgacaaagag tatattcctt
     5641 cgattaaacc atatttatgg tggaaatatt tagaagaaga tcattcgcgt gaggacgacc
     5701 gatatttcta tatcgattcg aatgtcattt tcaataaaag aattaatttg cgcaaattgc
     5761 cttctaaaga tgatgtttgg tattgtagcg actgctgtag ttatctaagt cttgattata
     5821 ttagaagctg tgaaaacgga gaaaatattc taaaagatat ggcaaacatt gtaaatgtta
     5881 cagtagaatc tttggaaact ataaacacta attcaggagg cgcacagtgg gttattaacc
     5941 gtcctaaagc gaattattgg aaaaaggttt atctggattc taatcggcta tatcgctacc
     6001 ttagagggca aaaaacaaat atacaaatct ggacagccga gatgtgggca cagctttgga
     6061 acatgatgta tttcaatatt ggtcctaaag ttcacgagga attagacttt tgttttgcta
     6121 ctgatccaat agaaaaagtt aaagaagtaa aaatcttaca caatgctgga gtaacaacaa
     6181 acgatgaaga tttatttttc aaagggagat acgtgacttc tacgcctttt gatgaagatt
     6241 tatcatttgt aaacaagaaa aaatgctctt acgcatatgt taaagcaatt aaggcggtgg
     6301 ttagatgacg cctgaacaag tgactgaaga attgctaata gctgtgaagg ataatattta
     6361 cgttacctgg aacgaagaag atgagtcaat taaaaagatg atagctaaaa atgctgttta
     6421 tcttcaaagt aaagtgagta caacactttc tttttctcct gaaagcttag aatacggatt
     6481 gctaatcgaa agatgtagat acgactggaa tcgtgcttta gatgagtttg aacaaaattt
     6541 cgctagtgag ttattaggtt tcattcaaca ttatgcgcta caagaatata ttgcaggtga
     6601 tgtgaatggc gaataatcgt agactcgaag aaacattcaa cgatggttgg ttaaagattt
     6661 tgacgcaaac taccaaaaga aatgaactag gaaaaaagat tggtgtagaa gatacagaaa
     6721 tcacttcttt aaaatttaga aatctttcca tgagagatag tgatataaca gctatggatg
     6781 cgatgggatc gaaattaact aagaaagtaa agactccatt tcatccaatc gccaagaaat
     6841 ttaataaaga tcaatatttt atcgtaatcg atagtatgcg ttacaacgtt atctatgccg
     6901 attacgataa tttttatatc tatttttatc ttgaaagtgt gggtgaatat ggtgattgat
     6961 aattctaaag aaaaagaacg tttaaataag caaatttctg ctatcaaaac ttccttagaa
     7021 gagcattttg gcctcaaact ctttcaagac tccgtaggcg aggatgagct acctgatgat
     7081 tttaattact tcattctcga aacaggagaa atagaaatga tcactgagcc aaaatatagc
     7141 gtgggtcaaa atctatatct aactttctat tcagaaaata gagaagattt aacaggagac
     7201 tcactagata ttatttcatt gattcaaaat cgttcgattc gttttcagag aatggatccc
     7261 aatcatttaa aactagagaa ccaagatcgc tatatcgatc aattggtatt tacgtttaga
     7321 cgattattga agagtgattg tcatggctaa aaatagttgg gagctaaaaa taaatggaca
     7381 tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac tgattaacga
     7441 agcattgaaa tcaaaaggtt cggctattgc agtggatagg attacagaaa aaattcctgt
     7501 ttctgaagca gatttaagaa gagggcacca acacgcaaaa aatagtcgtc cacttaagac
     7561 tcaatacatt aatttgggtt tcatcattag acctacaaga aaatttgagt atttaaaata
     7621 tcctgatttg gggataggta cttctaaaag aaatcagccg gacgaattta tgagaagagg
     7681 attaggtctt gcacttgatc caattacaga acttctgatt cgtcaattcg ataaattaaa
     7741 taaatagggg gaacaacaat ggctaaaaca aaaactgtag taacaacgtt cgataacgta
     7801 agtatcaaac gaattgcttt taattttaag aacgcaggaa atgcaatcgc aacgaattgt
     7861 aatggacaat tagatggcga aacagaaatg caaacggtgg ttaaaaaatg tggagcgaca
     7921 gaagtaaaat caaaatctaa accaatcaat atgacggtaa caattactgc acatgtaccg
     7981 atggaagttt atcgacgttt caatgggttg aaacaagatg gacgtattaa accaggcatt
     8041 tactcttacg gtcctgattc cgtaggcgaa gatttctcac ttgctgcaga gatcgtggat
     8101 gacttcgaag aaaatagaaa gttagttggt atgttagcat gcacttcgaa tacaggatta
     8161 acattctcta ttgaaaatgg tgcggatgaa gtagctgcgt tagaactaga aacaaaagtt
     8221 atgcaagatg aatttggtaa attttatcat gaagcaattg ttgcagaact tgaagaagac
     8281 ttaacagatc aatggatgac gaatctatct gctgatgtga ttaaaaaaac ttcagttgtg
     8341 acaactacgg ccactcaatc acagtaaaaa aaacggaggt agcgaaatga acgaagatta
     8401 ctcaaaaatt gaactaaacg atggaacaat tttgaattta gaacctaaac tgaatatcaa
     8461 gaaattattg atgatcaata gagattttaa cacagacgag tttgcaaaaa tgtcgatggg
     8521 aaaaggctct atggatatta cagttattca aggtgcaaaa gccgtatatg tagcttatcg
     8581 tcaagcgaac atggtcgatt acatttcatt cgatgaattt atagataaat gggattttga
     8641 tatggaggtt gcagtagctg tatacagtac tatgatgttc aaacaagcac gtgatgccta
     8701 tcaaaaagaa ttcgaaaaag caaataagga aaaaaagctt caaaagtaaa aatgccaaag
     8761 ctcttagttg aaacgtgggt cgatgtctat tcgatgttga ccgacgtttt ttctatgcct
     8821 tcagatttgg ttttaagcga tatctgttta gatgacattt tgcaaatggc ttacaacaag
     8881 agcgcttatg aaggatggaa gaactatgca ataaatcaat cccagaaaaa ctaaaggaaa
     8941 ggaggtaaaa aatggctaaa aagagaacgg aagcagaagt aactttcata gctaacgatg
     9001 acggattgaa atctacgtta aaagaaatca gtgctgaatt aactaaaaat agagcagaat
     9061 taaaactaga acaagctcaa ttacaacaga ctggttctga atcagacaag ttaggaagta
     9121 aattatcttc tttagaaaag cagtatgaat tacaaagtca aaaagttgaa gtaactagtc
     9181 aacgtttagc caatgccaaa aaatattatg gagaaaattc caccgaagtt cagaaacttg
     9241 aaagagaact gattaatcaa caaacagctc agcaaaggtt atctaatgat atagataaaa
     9301 cgagtaaggc attggctcaa tctaaaggtg aatttaaaac gtatgagtca actatgaaag
     9361 acttagataa tgagcaacaa catctaaagt caagcgcatc attagtagaa tccgaatata
     9421 aaaaatggca agcaactgct ggccagtctg ctacagaatc tgaaaaatta gcaaaagcac
     9481 aagaatatgt cggcaaacag agtgatattg ctgaacaaaa aatcgaagtg ctaaaaaaac
     9541 aattagatgc cacacaaaaa gaatttggag aaacatctac cgaagcttta gaaatgaaaa
     9601 ctaagcttaa tgatgcggaa agagaattcg aagaattaag taatgctgct aaaaacgttg
     9661 atacctccac agtagatgat atcggcaaaa aacttgatat gggtaattta atggaagcta
     9721 ctgatcactt gtcagtgatc ggagataagc ttattgatgt aggtagtaag tctattgaag
     9781 cagctggaaa agcacaagct atgcaggccc aatttaaaca agtctttggc tctttagaag
     9841 gggaagcaca ggacgccgtt gagggaatgg ctgaagaatt tggaatgtta ccaaatacga
     9901 tcaagcctgt ttttacacaa tatacgtcaa tgtttaaagg acttggatat gataccaaag
     9961 aagctatgga gttagctggt gatagtactc agttagcagc agatgcagca gctttttacg
    10021 ataagtctat ggatgatgct agtgaatctc ttaattcatt tataaaaggg aactacgaag
    10081 gtggggagca aataggttta tttgctaatg atactcaaat ggcagcttat gctgttaagc
    10141 ataatttgat accagcgact gaaggagcaa aaaaagccag tgaagagtca ttgttagctg
    10201 ttgaaaaagc acaatctaag tatgctgatg ctattaagaa acatggtgaa ggatctttag
    10261 aagcaagaga ggctgcttta aaacttaaag atgcgcaaga taaaataaat gaagaattag
    10321 gcccacaaac gcaaaaatgg tctgatttag atgaggctac caagcaagca gttcgagttc
    10381 aatatgctga agatatgcaa aaattagccg gtgctacagg tcaggctagt agggagtctg
    10441 atggtttaga aaaccaaatg acaagagcaa agcaagcgtt agaagatttt tatgcctcat
    10501 tgggtgaaga tatactgcct gtgtttatca aagggttgca agcaggagcg aaagctttgc
    10561 aaggattagc cgaatggtgg agtaaacttg atgggccaat gaaaaatttc attttagctc
    10621 ttggaggaat tctagcatta ttaagcacat tagctcctgt tataaccgca gttgttacga
    10681 tagttggcac atttggttct acagttttgc tgccaataat aggaattata gcgggcgttg
    10741 cagctgtaat agcgattgta attacagcgt ttcaaaactg gggcgcaatc actgactggt
    10801 ttagtgattt atggaaaaaa tttaccgatt ggttaggtga tacctgggaa agtataaaag
    10861 aaagtgcctc atcagtttgg gatggagtca aagaaacctg gtctggattt gtagattggg
    10921 ttcaagaaat ttggcaagga gtttctgatt ggtttggaga gttatggagc ggattagttg
    10981 aaggagcttc caacatctgg caaggagtcc aagagacttg gcaagcattc gttgattggg
    11041 tttcaaatat ttggaacgga gtcaaagaag tatggtcgat tatttgggca gacattgtag
    11101 gaattgttca aataccatgg acattaataa cgtcattgat tcaagctggt attaatatta
    11161 tcgtgggtat ctttgatgta gctggacagt tattaggcgc agcttggcaa gctgtttgga
    11221 cacctatttc tgatttcctt aaaaatactt gggatactat gacacaatgg ataagcatcg
    11281 cttggaatgg aattgtaact acattccata ctatatttga tccagtagtg gcatggtgga
    11341 atggtatatg gacatctatt agtactacgg cttcaaatat ttggaattca attagtgcaa
    11401 cagcttctag tatttggaac agtatcaaga atacaatcac tagcttggta caagcagctg
    11461 ctacagtaat tcaaaatatt tggtcaactg tatctagttg gttaggtgga atttggaatt
    11521 caatcagctc tacagcatca aatatctgga atagtgtgac tagtagtata agcaatgcta
    11581 taaacgcagc taaaagtgcc attcaaagtg tttggaatag tatatcttcg tggatcagcg
    11641 gaatttggaa cggtatcaaa aatactgctt tgaatctttg gaatggaatt acaagcacta
    11701 ttagctctaa agtaaacgat ggaaaaaatg caatttcaag cggttggtcc aatctaacag
    11761 gtattgtttc cgacatattc aataatgtta aaagtacaat tgctaacata tgggaaggca
    11821 tcaaaaagac tgttagcgct ccaattgatt ggattagaga caaaatcagt ggcatttttg
    11881 ataatttgaa tatttctata ccacatattc cgttaccgga atttatcatg gaaggcagct
    11941 ttaacccgct aaaaggtcaa atcccccatt tgcgtgtaaa atggcatgct aaaggaggta
    12001 tctttactaa accaacttta ctaggtggaa tgaacggtgt cggtgaagca ggacctgaag
    12061 cagttttacc tttgaaaaga tctgttttgc aagaaattgg tgatcgtatc ttgagtagca
    12121 catcagtttc atctagggca caaacgattc aacctgtgaa taactacgaa ttcaatttca
    12181 caattgatgg taacgcagat gaggttacta tgaagcaaac aactcaacaa atcattgata
    12241 gcattacaaa aattcaaaat gataatgctt cggcatggcg ttaaacagga gagtatttct
    12301 cctgtttttt tagtattaaa aaggatgtga aaaaatgact gattgtatac attctataat
    12361 cgatggattt cctgattatt tgcataaatt ggctttagct gaaagaccaa ccataccttc
    12421 tccaaaaaga cagagagttg aaacttctgt tttaggtagg ttaggtggct tagtacaaga
    12481 ttactcgttt gaagatatgt cgtttacatt gcactataac tatttagagg atgtggaaga
    12541 ccatcaagcg ttcaagcaat cgttttatat catgcgtcat tggttaaact atgcaaagaa
    12601 attagaattc tctgacgatc ccaacgtcta ttacgttatc cagactatcg atattgggga
    12661 tgcagaaaac gatattgttg aatggggaga gttcgatgta aatattactg cgaaaccatt
    12721 cgcaagagtt caagaagatg tacctataac cgtagataaa ccacagtcat ttaacttgct
    12781 gaataatagt ttagaagaaa gttttccaaa gattatcatc actccttcag ctacttcatg
    12841 ccagttcatc ttaaatgatt atgtgtttag tttcgaaggc ttagtagcag gaactgatgt
    12901 agtcattgat agtgatttga tgctttgcta cgaagagcaa tcggacggag atattttaga
    12961 tcggtccaac aaaatgaaga ccatgcaata tccaacgttg caagtggata ttaatcattt
    13021 taattgtact ggtttgagca aaatacaaat ttatcgtaat gggttaaggt aggtgaaata
    13081 gatgatcgat aatttaataa ctatttacga taaaaatgac gcgaataatt tagctgaaca
    13141 tttatatgat acgcaaggtt taggcgcttt gtcagactgg ttaacagcta ctgttagcaa
    13201 taaactaaac ggagccgaga tatttcaggg tacttattca ataaacggaa ctaatgcaga
    13261 tttgattgta gaaggacgta ttattcagtg ttatgtagat gaaaatcgag caaaacagcg
    13321 tctacggatt tattatgcaa aaacttctgt aataggcaat acgatagaag taaaagctga
    13381 acctattttc aatgatataa gaaaatcggt gttgaataaa tatgacagcg gaacagaaaa
    13441 gatcactgct actcaggcat ggcaaaacgc aaaagtttta gcgaaaccag ctatcccttc
    13501 gcagttttct ttctcgtcat tagtagatac gcttgctaat gtgaagatag aaaaggcgaa
    13561 ttttttagaa ttctttggtg gaaaagaggg atctattcta gatcgatttc atggtgagtt
    13621 tctaaaagat aataacacat tacgtcatga aaaaagtcta ggtacggatc ataaaatcaa
    13681 agcgatttat actaaaaact taactggtct tgacttagag attgatgctc aaagtgtttt
    13741 agttggagtt tatccattca ttagcagctc ttcagaagga gaagacgaga tcactctacc
    13801 agaagaagtt attttcacgg attacgtgga tgattatcct gctggatatg tttcttttgt
    13861 tgattttaaa gacaaagcga ctgatgtagc cacattaagg gaagctgcta aagactggtt
    13921 gaaaacaaac atagataaac aaaaaccaca agtgagtggt tcgattgaat tagtaccatt
    13981 gaggcatcaa agaggctatg aaaaatttgt tgatctagaa aaagtttcga tgggtgacgg
    14041 agtagatgtg tatcatccac agttaaaagt gaatatgtca gcgagaattg tggaatatac
    14101 gtttaatgtt ctaaccaatt catacgataa attagttgta ggaaacgtca aaacaaactt
    14161 cttagaaaac acagagaata atgtcagcaa tttgattaat gatgccattg atcaattgaa
    14221 aaatggtggc gaaatcagtg atttaatcaa tgatattgta gatcatcaaa ctgatatgat
    14281 tactggtcaa aatggtggtt acgttttatt agatcctaaa gaagccccta gtcgtatttt
    14341 gattatggac acaccagata agaatactgc acggaacgtt ttgcaaatca acaacgctgg
    14401 tattggtttc tctaaaactg gcattaatgg aacgtatgac accgcatgga cgttagatgg
    14461 cggattcaat gcctcgttta ttacagctgg tgagatagta gggattacta ttagaggtac
    14521 tacattaatt agtgatggca ctgattatag aacaagtata gctaatggca aaatgacttg
    14581 gtactcaaaa aaagttaaca aagatattat ggagctagaa gcacgtgatt atgtaagtgc
    14641 tgatgccggc attgtatcat acaccatgaa aactggtggt ggtttcatga ttagaaatcc
    14701 acagggtaac ttggttttta gtacgtggga taatggtaat aacagaccgt ttttatcttt
    14761 tggtgcgcca aatttcagat acagtaatgc tagttatata aatgatggtg acggtaattc
    14821 tttaggtatt gatggtagtg caggtaattc atgggaattt aaagtagctg gtaggactat
    14881 gaaatttact agtgacggta tgctaacgtt gccaggttgt ttttttggtt catgggaaga
    14941 tgggaaactt gctagatttg aacaatcaac ggtacaagta tataaagatt ttactgttag
    15001 aggtactaaa aactcaaccg taccaacaga acattatgga caacgactat tgaacgctta
    15061 tgaaactcca gaatattatt tcgctgatta tggggaagcc gttacaggtg atgatggtaa
    15121 agttcgtgtt gatattgacc ccatgtttgc tgagacagta aatctaagtc ggtatatgac
    15181 acatgtgaca cctacagaac tagttttgtg tgctgttact catgaagatg ttgaccattt
    15241 catcattgaa actagtaagc caaacgtatt ggttagatgg aatttagtgg cacatcgact
    15301 agggtatgaa gatgttagat taaaagagga tacagcatat gatagcacag tgcttgacca
    15361 aaaacgtttt taaaacgaag acaaggaggt atataaatgg ctagcagttt atataatttg
    15421 gctttagatt tcagcaaaga attaaactac accaaagcta ttatggctcg tcaaggtgat
    15481 aaagggatca cggtgactgt taaaccgttt ttaaa
//
LOCUS       DACGLH010000065        15282 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.069, whole genome shotgun sequence.
ACCESSION   DACGLH010000065 DACGLH010000000
VERSION     DACGLH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.069"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..375
                     /locus_tag="IXT21_002143"
     CDS             <1..375
                     /locus_tag="IXT21_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4869526.1"
                     /translation="RLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEG
                     FQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAF
                     GYRNFYNFRARIYLQQGLIFEN"
     gene            complement(758..1486)
                     /locus_tag="IXT21_002144"
     CDS             complement(758..1486)
                     /locus_tag="IXT21_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4869527.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1464..3038)
                     /locus_tag="IXT21_002145"
     CDS             complement(1464..3038)
                     /locus_tag="IXT21_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4869528.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3267..4205
                     /locus_tag="IXT21_002146"
     CDS             3267..4205
                     /locus_tag="IXT21_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4869529.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4217..5128
                     /locus_tag="IXT21_002147"
     CDS             4217..5128
                     /locus_tag="IXT21_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4869530.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            5152..6624
                     /locus_tag="IXT21_002148"
     CDS             5152..6624
                     /locus_tag="IXT21_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4869531.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6675..7286
                     /locus_tag="IXT21_002149"
     CDS             6675..7286
                     /locus_tag="IXT21_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4869532.1"
                     /translation="MNENKWIKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFQILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7316..9229
                     /locus_tag="IXT21_002150"
     CDS             7316..9229
                     /locus_tag="IXT21_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAQ4869533.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLVLMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9357..12338
                     /locus_tag="IXT21_002151"
     CDS             9357..12338
                     /locus_tag="IXT21_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAQ4869534.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12335..13996
                     /locus_tag="IXT21_002152"
     CDS             12335..13996
                     /locus_tag="IXT21_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAQ4869535.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14036..14968
                     /locus_tag="IXT21_002153"
     CDS             14036..14968
                     /locus_tag="IXT21_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ4869536.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4967 a         2428 c         3063 g         4824 t
ORIGIN      
        1 cgactgttta aacgaccaat tagtcaaaca gaaatcgttg atgagctgtt aagctacaac
       61 gaagaattgc gaactgcgta tcatttttgt caattattac gctattattt cgttaaacga
      121 ggtacggaag gctttcaaga aacccttaaa acaatttcat ctacgttacc tcagtcgttt
      181 aaaaagaaat tcacgatctt ccattgctac caatcgggta tttcaaatgc ctttaaggtg
      241 tcccactcaa acggcgtcac tgaggggctg aataacaaga tcaagctgat taaacgaatt
      301 gcgtttggtt atcgcaactt ttataacttt cgggcacgga tctatttaca acaaggtctg
      361 atctttgaaa actaaaaaaa tatgcggcgc acagaacgcg ccgcatatcg gactaagttt
      421 tattcaaatt tgtcttcacc aacacgattt gacgaagagc ctatttaaat aactacatcc
      481 atttattcta tcttctaaaa tagccttttt catttcagtt aaatagttca gcgccatttt
      541 tgcactcgac ttgcatagta gttttttcca tattcctcaa cctcagcggg ggttgaagac
      601 ataaggaaaa tcctatgcgt tatgctcaac tggatctgat taaatattct aatgtatttt
      661 tctctaccat cattaaatgg ttaatagcca aaaatcacca cttttcaatg gctccttgtt
      721 gtataagtat aactagaaga tgaacacgtc tgagaaatta tagaagcttt ttacggtaat
      781 tactggggct gtgtccagta gtttttttga agagaacaga aaaatagctc acgtgatcta
      841 ttcctacttt ttgactaatt tcatcaatag ataagaatgt gtctttcagt agagcacttg
      901 ctcttttgat tcgaaagtct agcaagtaat catgaaagga ataacctgtt tcttttttga
      961 aggcccgtcc taaataagcg ggatggtaat ggaattgtgt agctacatct ttgagcgtaa
     1021 gcttttcact ataattgttt tgaacgtaat caatcacttg ttgaattgac tcattaaaag
     1081 aatcatcagt tagagagttt tgattagctg ctgatttacg cattttttct tcttgtacta
     1141 acttaactaa ataaggaaaa tcaatgggtt tcgttatgta acttttaata gataaatcaa
     1201 tccccttttt tgcatactga aaattttggt gggcactaat gacaatacta gtaaagtagc
     1261 gattggatgt ttgttgcaaa gattcgatta attcgagtcc agaaattttc cccatcgtaa
     1321 tatctgtgat aagtaaatca aagcgctggt tttgcaggtt tttaaatgct tcgtctccgt
     1381 ctgtgtagca gtgatcgata atgatgtcgg ggactttctt caaaagataa tgccttatgc
     1441 cttctacaat tgtacaatca tcgtcaacaa ttgcaattct caacatgcat attcccccct
     1501 ttttgtattt tgatagtaat cgttgttccg acgtgtagtt ggctttctat aacaaaacgt
     1561 gtatctccag gatagagatg ctttatacgt cgaaaagaat ttgagatgcc gatattctca
     1621 ctataaggaa tatcatcatt aagtttttgt tgaatatgct ccagcttgtc ttggtccatt
     1681 cctttcccat tttcaacgat ttttaacaca taatggttgg ttgtttcagt tgcatagaca
     1741 gtcaaacgat tttccttttt tgatgaatcg aagccgtgtt taatataatt ttcaacaaat
     1801 ggctgtaaag aaagttttgg taacattaat tgatcaatat gatcaatcag ctgatagtca
     1861 aagttatgtc gataacgacg ttgatgtagt tgcaaggaga ggtcgatatt aattaattcc
     1921 tctttcaaat caattatgtc gggctgatat gtattattgc gatacaactt gctcaataaa
     1981 tatatactgt cgctgatttc ttcgtcatca ttcaatatgg cagtcattcg aataaattcc
     2041 aaattattaa atagaaaatg aggctcgatt tgatattgaa gtgctgcata ctttgcttct
     2101 tgcttcaacc aattattttc ataaagtgtt ttttgatatg tagcattatc taaataaaac
     2161 tgattgatat gcatagcgat ttctttcaaa tcaccgactt tgttttcgat attgattgac
     2221 ttgtagtctc ctgtagatga atgatttaag gaagtaatga tttcctgata ctgctggata
     2281 tagttacgaa aacttctatt taataattga tgaatagcaa gaataaacaa actcattaat
     2341 aagaaaatca tgattgccag acctaataag gtttgtgacg aataatttgg attttcatat
     2401 acttgtaatg tgtggttacc gatagctatc tgttgcgttt ttttccattt agaaggcgag
     2461 atgtcttcag ataaattgac ggaatcaata ccattagaca aagaataatt gtaatgtgtt
     2521 ggaaagagac tcttttttag cgcgttgatc gaaacgtcta gaatcaatgt agcatcgggc
     2581 atgtcctcac taatgaaact taatgaacga ataaaagaaa ttttttcgtt ttttgtaggt
     2641 gcgtaggcac ttaagtattt ttcgtaatct aatgagatag atatattttt aacttcatca
     2701 tcttgcacaa gtttttttaa gttatctact aaataggtag gggagcgatc ctttgacaaa
     2761 atatcttcca tgtattcagc aggtgatgag aggtagtagt cattaaggtt ttcttggaaa
     2821 tcataatttt gatagatctc tgtcacgata ccggagagaa tacgattata tgtttccata
     2881 tagtgggtga tttgctgaat gttttgcagg ttttgatttt tttctttttc aatcacattg
     2941 atataggaaa agaacaatgt gaaacaggca agtaaagtaa taaaaaaaat caatatgtat
     3001 gtaaaacgca taataacttt tgataaatcg tttttcattc aaagtcctca ttttgataat
     3061 ctctaatttt atgaatcatt atagcacata ttacaagtga tgtaaacgct ttgcaaacag
     3121 ggctacttat gattgtgaat acagtttccg tttaaaaact attctattgt agaggtttaa
     3181 taaactaaaa ataatgtata aaaaattaat gtaagcgatt aaccaagtgg gtataatcaa
     3241 ggaaacgctg agagaggaga gacaaaatga agctaaagaa agggttatta ggaaatctaa
     3301 aaaaaaaccg agcattgttt attatgcttt taccaggtgc gttatggtta tttatatttt
     3361 gctatatacc aatgtttgga gttgtaatcg cttttaaaga tttcagaatt tcatcaaggg
     3421 gattctttac aagtttgttc aacagtcctt gggttggctg ggaaaatttc aaatatctct
     3481 ttgcttcgca agatgcttgg atcattactc gcaatacggt tgtttataac gttgtaggta
     3541 ttttgctaag tactttttgt acagtgtcat tagcaatcat tctgagccaa atggcgaaca
     3601 aacggttatt gaaaaagatg cagacattta tgatttttcc taatttttta tcatgggtag
     3661 tcatcagtta ctttgtttac gcatttttga gtacggataa aggattaatt aatattttac
     3721 taagcaattt tggtgtggaa cccataaatt ggtatagtga accgaagtac tggcctttta
     3781 ttattgtttt tacgagttta tggaaaggcg taggctatgg atccatcatt tattacgcgt
     3841 caattatggg gatcgatact agttattatg aagctgcttt agttgatggt gccagtaaat
     3901 ggcaacagat aaggaatgta gtcttgccac atttaagatc gattattgtc attatgttga
     3961 ttttgtctgt tggtagtatt tttcgagctg attttggatt gttttatcaa ttgccaagaa
     4021 attcgggggc tctttatgat gtgacgaatg tattagatac ttatgtctac cgtggattaa
     4081 tgaatagcgg agatatcgga atgtctacgg cagcgggatt atatcagtca tcagttggct
     4141 ttgtcttgat tttgattact aataagatag tcaacaagtt agatcctagt tcagcactat
     4201 tttaggagga atcaaaatga tatcaaaatt aaaacggcag atgtactttt cgctgccagt
     4261 cgaacggttt ttcacaatat tactaatctt atttactctt agctgtattt ttccctttat
     4321 tttcgtaatc gtcatttcat taacagatga aaaagcatta caactcaacg gttacgaatt
     4381 attgccggct caatggagca ctgatgcata tcaatactta atacaagatg gcggacagtt
     4441 acttcggtca ttaggcgtta caattatgat tacagtgatt ggaacgttga ttactgtttt
     4501 tatgacggga acctatgctt atgttttatc aagagcctct ttcccttacc ggaagttctt
     4561 tactttttat ctttttttta ccatgttatt tgcgggaggg atggttcctt cttacttagt
     4621 gatgacgaag atgcttggct taaaaaatac aatttgggct ttgattctac ctcttgcttt
     4681 tagcccatac aatgtcatta ttttaaggac attctttaag aagtcgattc cagaatcgat
     4741 tatcgaatcc gctaaaatgg atggctgtag tgagtttcga gtttttttcc aaatcgtttt
     4801 gcctttagcg atcccagggg ttgcaacaat aggtttattt tcaagcttag gttattggaa
     4861 tgactggttc aacgcattgc tgtatattga caccaacaaa ttgattccat tgcaatattt
     4921 gttaatgaag attcaaaatt caatggaatt cttagcaaat aataatgaca ttacattagc
     4981 gcagcaacaa gcaattcaaa atagccttcc tcaagaatca acacgaatgg caatggtcgt
     5041 agtagctact ttaccgattg caattgttta tccgttcttc caaaaatact ttgttcaagg
     5101 attgacgatc ggtggagtga aagaataaaa aagaatagga gatgttaaaa gatgaaaaag
     5161 aacatgcaga aatatttgat tggtggtgct tctgtactaa cccttggcct attaggtgct
     5221 tgtggtagca gtaatggcga agcaaataat gaaacaggaa ccacaaaagt tacttggtat
     5281 caggttggag aagaacccaa agattttgat gaggtcatga caaaagtcaa cgaaaaatta
     5341 aaggaagaaa tcaatgtaga gttggatatg cggctgattc cggacgggga ttatcagcaa
     5401 aagcttggtg tgatgatcaa ttcaggagaa gaatatgata tttgtttcgt caatggcaca
     5461 gattatgtga attatgggaa caaaggtgct ttcattagtc tcaacgatca tttggataaa
     5521 gagttgaaag attatgcagc tgaattgaat gaaggattta ttgaaggtgg ggcaattaat
     5581 ggcgaaactt atggattgcc agtcaataac gatattgcca caacggaata ttggaccttt
     5641 aataaagatt tggtagatca ataccaattg gatgtagaga atgttgaagg tgtagaaaca
     5701 ttggctccag tattagaaaa gtttgcagaa aaaaatactg atacgaatat tacgccaatc
     5761 ggtatgaccc gtaccagtgt ctgtattcct tatgacaata ttatcggtgg tggtgtaccg
     5821 ctagcggttg catttgaagg cgatacacaa acagttgtta atacttatga gactccagaa
     5881 atgatgactt atttgaaggc tatgcatgag ttttataaaa aaggatatat tttgaaagat
     5941 gcagccactg ttacgacaga tccatttcga attgatagta aaaactggtt tttaggtcgt
     6001 aactcaagtg gttatcaacc attagcacaa aaaaaattag aagctctagc gggacatgga
     6061 ttaattttaa aaccagctgg aaaagctttt aaaacgactg gatttgcaag agtggcaatg
     6121 catgcggtat ctgcttcaag taaacatccg gaagcagcct tgaaattgtt gaattatgtg
     6181 aataccaatc aagaagtagc ggatttaatg gtttatggaa ttgaaggaaa acactatgat
     6241 cgcaatgaag atggttcgat tactttaaca aactctgatg attattatca aggcgcttgg
     6301 gcattggtaa atacttatct agtttcaaaa acttcaccga ttacagaaga aagtaaaaaa
     6361 ctagaagaag aaagtaaaaa gtttcaaaat gaagcaccga tgtctcctat tttaggtttc
     6421 tcttttgacg cttctgattt tcgaacagaa attgcaagtg tacaaaatat cgtgtcacaa
     6481 tatgaagtac ttttaagctc gggaacagtt gaccctgaac aatatgtacc agagttgtta
     6541 gaaaaactag aaaaagctgg attggataaa attcaggcat ctgctcaaga acaatacgat
     6601 gaatggcgaa aatctaacaa ataatttttt actaaatgaa aagaatagac taagcagtta
     6661 gtggggggat tcaaatgaac gaaaacaaat ggatcaaagg atttaattgg gtttttcaat
     6721 tgttgttact aaactttact tattttatac taatttgttt aggatttgga attttaggtt
     6781 ttttccctgc tacgatagct ttgattgctg gcgttgtgaa tcgaaaaaaa tatggcttgc
     6841 aagagtctgt gaagttcatg agtaaaatct ataagaaaaa ttttgttaga tataatctat
     6901 attgctggtc agcaactttg ttgttggccg gcaatattac tacgatctgg ctttgttggc
     6961 agcaaagcgc accattgttt tatttattaa gtacattatt atcggtaatt acactgttcc
     7021 tagtctttgc aatgttgaat tgggtcgtga tcaaaagtct tgggttgtgg atgatgggag
     7081 accgactgat tgactatttg cttttcacag caacgatgtt gccaacggta gtcatgcaag
     7141 gcttggtgct tggaatttat ttccaaatat tgatgatttt gcctgtgatt tttctgtttg
     7201 gcggaacagc tgtaacggta atattgatta gtaaattaac tgaaagagcg ttagcaaagt
     7261 tgccttgtca gatacaatcg cactaatttt ttcacaaaaa ggagtatttc tttctatgaa
     7321 gttaactgat aaacaagtag ctaatatagc ctttatccag caacttgaga ttccattgac
     7381 acaaaaagat gaagaacaaa tagacttgat ttttactgac acaaaggcta atgtgcgaca
     7441 aatcaagatt actaaaaatg agcagcaacg aatctgcttg tcttattcac atgaagactt
     7501 gctgtatcga ggattgttta tctgccttcc acgtttttta gctcaaggag gagagatgcc
     7561 atacattgaa gaaacctcga tcattgagac cagttattca ttagatgctt ctcgtaatgg
     7621 cgtgatgaat aaagagactc taaaaaaatt catttcttac ttagtgttga tggggtacaa
     7681 cacattgtat atttacacag aagataccta tgaagtagaa gaccttccat attttggtta
     7741 ccttcgcgga gcgtatacaa aagaagaagt gaatgaaatt gttgaatttg cttctggttt
     7801 tggcatagaa gtagttccat cgatccagac ccttgctcac ttgacccaat ttttgaagtg
     7861 gtatgctcaa caagaactga tggatgatca aaatactcta ctgatcggtg aagaaaaggt
     7921 ttatgcggca atcgaaaaga tgattattgc ctgcaaagaa atgtatcaga cagagcgtat
     7981 tcatttggga atggatgaag cctatcaagc aggtctggga cgatatttaa gcctgtatgg
     8041 gtattgtgat cggacaactt taatgattca acatatggac cgtgtgttat caattgttga
     8101 taaatataag atgaaaccaa tgatttggag tgatttcatt tataagctat tagatgaaac
     8161 cggtaaagat aggtattaca atcctgattt taaactcaaa gcattagcag ctaaaggatt
     8221 accagcagga ttaacctatg ttcattggga ctatggttca gatgaagttt cctcctatga
     8281 gaaagtaatt gataaccatt tgaaattttg tgacattgat cattacgttt tagctagtgg
     8341 tgcccatatc tgggggagac tagccccgaa tcatgggaaa acagaagcaa ttgtttctgc
     8401 agctgtaaaa gcttctaaaa ataaaggtgt acgttctgtg atgttaacta cttggggaga
     8461 tgaagggcag gaaacagagc attggcattc cttaattggg gcattaaaat tagcagatta
     8521 tgtctattct gaagaagagt ggaatgattt tgaggaaatc tacgatcgtt tattaggaac
     8581 aggtagttat caattaatgt ccgaattacg atatttcgat gaagtggata ccattacaaa
     8641 taacaactta caaatggctt ccgtgagtaa actgctactt tggcaagatc cgttagttgg
     8701 tatatatgac tttcatgtag ccgctcataa caaaattgcc actagaacaa ttggtactta
     8761 ttatcatgag ttagctcaga aattagatat gaataaaaat tggaatccaa aacaaccaat
     8821 tttaacaact attcgtcaac gatatatact tttagctgaa attttagcac aaaaggcaga
     8881 actaggcatc tatttacaac actgctgtct ggtagaagac cttgcaggat taatggaatt
     8941 agcgaatcgg tgtgaagcac tagccaatcg agtaaatgaa ttggcatcta ttcatgaaat
     9001 aatttggtat caaacataca aaacctatgg ttgggaagta ttagaacaac gatatgctgc
     9061 attagctcat cgtctgaaaa ccgttgcgcg tcgtattaaa aatcatcttc aggggcaaga
     9121 tgccctagag gagcttttgg aagaacgatt accgttttgt caatatcaag tgccgcttga
     9181 aatttctggc tttaattatc ggcaaagctc ggtgtcaggc tacaattaac gaaggaatct
     9241 tcccgttgaa cggaccactt gctcatctaa tgggaaagtg gttcgttttc tattcataag
     9301 tagcttacca ctaactcttt tattaagaag agaaaaaaag taggaagtga gaaaatatgt
     9361 tatcggacta ttatcaaaat ctgtcagtta taagtgtcaa tacagaacca ttacgtaatt
     9421 attatattcc ttatgcaagt aaagaagaag caaaacaaga gttagaaagg gaaaaaacga
     9481 gccagtttat cgatctcaat ggtacatggg attttcagta ttatccttct gtccaacagt
     9541 taccggaaaa tttttttcaa gaggccttta ctgaaagcta tgatagaatt tctgttcctg
     9601 gagtttggca aacagaggga tttgacttac ctcagtatat caatgtatct tatccaattc
     9661 cttttgatcc gccttatgta cctaatgata ccccttgtgg tgtatatcgg cgtcagtttc
     9721 aaattgagca aacagatgca cgcttttact taaatctaga aggagcagat agtaattgtt
     9781 ttgtttggat aaatagaaac tttgtaggct atgaccaagt gagtcatagt acatctgaat
     9841 ttgatgtgac caagtattta caggttgggg ttaatgaaat tcaaattcta gtgatgaagt
     9901 ggtgtgatgg tacctacttt gaagatcagg ataagtggcg aatgagcggt ctatttcgtc
     9961 cagtgtatct tcttcaacga ccaactatgc atctgacaag ttatcaacta cggacgttga
    10021 ctgatcctaa aaaaggaagc ggaacattaa tggtttcttt tcaacgattt actcctattg
    10081 aagtatcgtt acctatttct tacgaattat tccatcctga tggacgtctc atagcgaaag
    10141 gggagtgtta ctcggaacaa caaataaatt tggatgttga agattgcctt ttttggacag
    10201 cagaaacgcc taaattatat acattacttt taacatgtaa tggtgaatat tttaaagaaa
    10261 aagttggctt tcgagcactg acgatcaaga atcgtaagtt attgctgaac ggaacaccta
    10321 ttaaattata tggagtcaac tatcatgacg tgaatcagca aggcacgcca gtgatgaccc
    10381 gagagcagtt acgtgcagat ttgttattaa tgaaaaaatt aaatgtgaat actgtacgaa
    10441 cggcacatta ccctaaatca ccagattttt atgaattggc ggatgagatt ggtctgtatg
    10501 taatcaatga agcagatttg gagtgtcatg gtgtggttaa tctatatgat actgaagcga
    10561 attatcaact aattgctaat gatccgacat tcgaaaagcc gattatggac cgagtgatcc
    10621 gttctattct tccttatcaa aatcgaagct ctatcattat gtggtctatg gggaatgaat
    10681 ctggttatgg agtaaatttt gaaaaagctc aagctttagc acggaaattg gatgattcac
    10741 ggttgattca taatgaacgc tcgattgaac cagttattga ccaagtgaat gattttagta
    10801 acttggatgt tattagtcaa atgtatcccc cactggaaga tgttcatcgt cattgtcaaa
    10861 gaagtgatac tgaaaaacct ttttttatgt gtgaatatag ccacgctatg ggcaacggtc
    10921 ctggagatct taaaacgtac ttcgatttga ttcaacagta tgattccttt cttggcggtt
    10981 gtgtctggga gtggactgat catgcaatca atagaggaac tttagaaacg ccatcttatt
    11041 tatacggtgg tgattttggt gaaaaatatc acgacggcaa tttttgcgta gatggtttat
    11101 tgaccccaga tcggcaatta acagtaggtg cattggagta ccgtaatatt cattcgccta
    11161 ttcaactgat tgctgtagat gaggagctgc aagaagtaac cttgagcaat ataactgctt
    11221 ttacagccgg ttcagcattt aacctttctc ttgaatggta tatagatgga aaaaaaacag
    11281 tttatgaagt aatgaatcct ttcatacttg agcctggcaa aagttgttca ttgcccatca
    11341 cagtacctac aacgattcat ggtgctcata cggttgctgt ttcgctatct gtttataaaa
    11401 atgatgagct ttatggacag caacagctct ttcttaaaaa agaaaaaatg agattgccaa
    11461 gaactgctca tcataatgat gcaccacaaa tttcagtaaa aggcacagag ctgataatca
    11521 aaggagaatc ctttatttat atatatcaat tagcaaaagc tgcgttcact tctgtaaaga
    11581 tcaatcaaaa ggaacttttg gaacgtccta ctcaattttc aatttggcgt gcaccaacgg
    11641 ataatgatcg taacttagta aacaaatgga aagcagctgg atatgctgaa atgtctttag
    11701 aaatcagaaa aaaagactgg aaattcgtag atgatcatct cctgatcgaa acgtcttatc
    11761 gaggagttat tccaggatta cagccaattt ttacaatgca catacagttg aaaataaatg
    11821 gtgcaggcgt aatcaaccaa aaagtatcag tggagaaaaa tccgattttt ccagatttcc
    11881 ctcgctttgg gatggactgg ccgttgaatg atactttttc gaaggtttct tattgtggct
    11941 atgggccgta tgaaagttat cctgataagc actatgctgc gatgtttggt atttatgaga
    12001 tggagattga ggaatttgat cagccatata tcaaaccaca agaatatggt aaccgctggc
    12061 aatgtgattg ggtatcaatc aaaaatcatc aaggatctac catgacggtt tatggaaact
    12121 ctaatttttc tgctctgcct tatagtcaag ctcaattaac cgaaaagtca caccattatg
    12181 aattaataaa agaaaataga acttggttca atttaagtta taaacaaaat ggtatcggct
    12241 cacacagttg cggtccttat ttagaagatc gtttccgttt tatggaagat acttttgact
    12301 ggaatatagt gattactttc aaaggagaaa atagatgaaa gaaacaaaga tcaatcaagt
    12361 caaaaaaaaa ttatctcgtt atcaagaagt tgtttatcga aaccattgct taaaatggtc
    12421 aaaaaataag ctgaatgttc gttttaatgg attattttat cgtttcgaac agttagaaga
    12481 agtctggaaa ccggtaacga atttccaatc atgtcctgta cagctcataa atgatccaaa
    12541 agaaacatac gatttatgta taacacacct ttataaccag catttgaaaa atgacggatt
    12601 ttatttaaag gttactaatg acaatcaact tacgattgaa tcagcaaacc ttcgagggtt
    12661 tcaatatgct atggaagcct tattagagag ttttttttcg gtaggagatc aattattatt
    12721 accggaattg attctgaaac atgaaccatc agtgaaaatt cgaggaatca ttgaagggtt
    12781 ttatggtatc ccctggacac atgaaatgcg cctctctctt ttttcatata tgaaagataa
    12841 ccaattgaat acttttatgt atgcccctaa agatgatgaa ttattacgta aaaaatggcg
    12901 tgaactctac gatgcgcaag aattagacaa atttaaggag cttttatctg ctgctgaggc
    12961 tagcaatatc gatttttggt acttaattag tcctggaaat gatattgata ttacgtgtga
    13021 agaagacatt caagttcttc tgaaaaagtt agaacaactg attgagcttg gcgtatttca
    13081 atttggtcta ttaatggatg atattgacta tcaattaaaa ggtgctgcta aacgccgttt
    13141 tcgagagcca gcattcgctc atgcctattt agtgaatcga gtagaggaat atcttagcac
    13201 tcggttgtcc aatcatgaat tagtcatttg tcctacagaa tatgataatc gtcatggttc
    13261 tcgctattta gcaacgttaa gtcaagaaat accagaacaa ttaccgttct tttggactgg
    13321 cccaagcacg ttagcggcgt caatttctac agaagagctg caggaaatgg cttctgtcta
    13381 tcaacggccg atgctgattt gggataatat tccggtaaat gattatttag aggataaaga
    13441 actattgttc atgagtccat atgagaatcg aacaccgaat ctttctaagg agagatacca
    13501 ggttacagga gtagtttcga atccaatggc tcaattagaa gcttcaaagt ttacgatcaa
    13561 tagtatggct aattacttat ggaactgtga gcgttttgat cctttggaaa cttggacgtc
    13621 agtcgttgaa aaagtagttg gaagtaaact acaaccagct tatcttacct taaccaatgc
    13681 ttttccaaat cattacacaa gcagtctgct ttcagatgaa gaattactgc tagccaaaga
    13741 cccagaatgg gtcacaaaag aaatggctgc tttagtccaa gcagtaaaat ggatccagca
    13801 agaacaacat acatctagat tgagaacaga attggagcct tggttacaag caatcacaga
    13861 aagttggact ttctggcaag aatggcaatt aaaaaaagat ccaaaagaag cagaagaatg
    13921 gttggctaag aaaaaaacac accgcatagg acgtgattta gtaactgccc atttagagca
    13981 aattatcaaa agttagatat cttaaaaaat aaaaagcaag aaaaaggaga acaaaatggt
    14041 caagagatta attagtgcaa actacagtga tgtcaagaaa atgacgtcaa aagagttgaa
    14101 gcaatccatc aaagcgagtg aaggacgggt gattgtttgt gaaacggttg ctaatactct
    14161 tccacaatct ggagaaatta cgaatgcaga agttgcgtca gcatttggtg cagatttgat
    14221 tcttttaaat ggtttcgatt gttatcgtcc tgcagttttt ggactaccaa atgtgacgat
    14281 tgagaaggtt tttgatctac agcaaacgat tgaaaacccg attcctaaat taaaagaact
    14341 agtcggtcgt cctgtgggaa ttaatttaga acccattgcc cctaaaacaa atatgatgag
    14401 tgagaaagta gaaatcagcc aaggaagagc aagctctgtt gaaacgatac aaaaagcaga
    14461 agaactagga gtcgatttta tttgctttac cggtaatcca ggcactggag taaccaattt
    14521 agaaattact aaatccgtaa aaatggcaaa aaagtatttt tcgggattga ttattgctgg
    14581 gaaaatgcat agcgccggct cagaagagcc agttgtgaat aaagaggcga ttatttctta
    14641 tgcggaagca ggcgcagata ttttactagt tccttcagta gggtctatcc caggcttttt
    14701 agagcaagac ctaattgaag cggttcggtt ggcaaaaaaa tatgatttgt taacgatgtc
    14761 tgcaatcggt actagtcagg aaagtgcccg accagaaaca ttgcggcaat tagccattat
    14821 gaataaaagt tgtggtgttg atttacaaca tattggagat gctggatacg ggggattggc
    14881 accagcagaa aatattttcg aaacctcggt tgcaattaga ggaatgcgac ataccatcaa
    14941 ctgcgttagc cgctcgatca atcgctaaca aaattaaata aaaaatggtt aaattgatta
    15001 tttaagtggg aacatacttg agatatcagc taacttctgt cactttaaaa aagggactgt
    15061 gacagaagtg aaaatagaca aaaaatatcc gaacaagaga ctaggatagc agaaaaacta
    15121 tcgttttgca acgaggacca actggaccgt aggagcaatt ctcttattgt tcggatattt
    15181 cgcttgttca aggacttatg tctgtagtgc ccttttttgg taggaaaaat ggtagggaac
    15241 catatcattt ttaatgaaaa tcaagcattt caaaagttga aa
//
LOCUS       DACGLH010000066        14955 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.071, whole genome shotgun sequence.
ACCESSION   DACGLH010000066 DACGLH010000000
VERSION     DACGLH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.071"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            105..614
                     /locus_tag="IXT21_002154"
     CDS             105..614
                     /locus_tag="IXT21_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4869537.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            707..1869
                     /locus_tag="IXT21_002155"
     CDS             join(707..983,983..1869)
                     /locus_tag="IXT21_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ4869538.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2149..3129
                     /locus_tag="IXT21_002156"
     CDS             2149..3129
                     /locus_tag="IXT21_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4869539.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3251..4669
                     /locus_tag="IXT21_002157"
     CDS             3251..4669
                     /locus_tag="IXT21_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ4869540.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4740..6209
                     /locus_tag="IXT21_002158"
     CDS             4740..6209
                     /locus_tag="IXT21_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4869541.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLTNRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6219..6686
                     /locus_tag="IXT21_002159"
     CDS             6219..6686
                     /locus_tag="IXT21_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ4869542.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6722..7594
                     /locus_tag="IXT21_002160"
     CDS             6722..7594
                     /locus_tag="IXT21_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4869543.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7675..10209
                     /locus_tag="IXT21_002161"
     CDS             7675..10209
                     /locus_tag="IXT21_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4869544.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10365..11138
                     /locus_tag="IXT21_002162"
     CDS             10365..11138
                     /locus_tag="IXT21_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4869545.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11131..11922
                     /locus_tag="IXT21_002163"
     CDS             11131..11922
                     /locus_tag="IXT21_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4869546.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            11932..12423
                     /locus_tag="IXT21_002164"
     CDS             11932..12423
                     /locus_tag="IXT21_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4869547.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12441..12851
                     /locus_tag="IXT21_002165"
     CDS             12441..12851
                     /locus_tag="IXT21_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4869548.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12855..14255
                     /locus_tag="IXT21_002166"
     CDS             12855..14255
                     /locus_tag="IXT21_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ4869549.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14278..14657
                     /locus_tag="IXT21_002167"
                     /pseudo
     CDS             14278..14657
                     /locus_tag="IXT21_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         5205 a         2100 c         3000 g         4650 t
ORIGIN      
        1 ggataatcag ctttttattt attttttctt ataaatttga taaatagaaa ttagtatagc
       61 cctaaacaaa ttatttgtgt tttattgttt agggctatac tatattggta caaggaatag
      121 aaagaggtgt ttatgtggtt gataaaattc agtttaatca agttatagaa gaattcatca
      181 aagtaaataa tttaatgacc catattcaaa aacgaccaat cagaataact aatgatctaa
      241 aactcagtac gagtatgatt catttgattg atgtgattgg aaattatcct gaaactagta
      301 ttacggaatt agctgatcga ttaggcgtta ccaaaggagc tgtatcccaa cagattccca
      361 ctttagagaa aatgaatctt ataagcattt ctcaaaaaaa aggaaataag aaaactaaac
      421 ttgtatcgtt aacaaaatat ggtcgaaagg tgtacgaatc acataattat ttacatgaag
      481 agctttatag ctcaataaat gaagcgttag aatcattctc accagaccaa atttcaacca
      541 ttaaacagat tctagaaaaa gtatcttata caatcaatga atatcaagta agtctgacaa
      601 gaaaggaaga ttgacatgaa aaaaatgatt tttgaatcgg ctacactaga ctagacagaa
      661 aaaataaggt gtgtaaaata aatgaaaaca cactggagga atttttatgt caagacgtca
      721 acgtagaacc tattcaaaag aattcaaaca acaagtcgtc gatctttatc tcgcaggaaa
      781 gccccgagct gaaatcattc gagattatga gctgactacc tcggcctttg ataagtgggt
      841 caagcaagcg caaactactg gctcgtttca ggaaaaagat aacttgatcc ctgaacaagt
      901 agaactaatt gcattacgaa agaaaaataa acagcttgaa atggagaatg acattttaaa
      961 gcaagcggcg ctgatattgg gacgaaaaga caagtaattg ataccaataa gcacaaatac
     1021 tcaatatcag cgatgtgtaa agtgttaaac atctcttgcc aaacctatta ttatcaagcc
     1081 aaaccagttg aagatgaatc agaactggaa gaactcgttc aagaagaatt tattcgcaat
     1141 cgaaaagctt atggtaccag gaagctgaag cagtgcttgg ccaatctagg tcttcaagtg
     1201 agtcaacgca gaatcggccg tatgatgaaa cagcgtggct tgcgttcaac atacacattg
     1261 gctcatttca aattccattc ttctacttgt aacgaagcca agaccgccaa tattctagac
     1321 ggaaccttcc tacaggaaaa gtcattagaa gcgattgtaa ccgaccttac ctatgttcgt
     1381 gttgggaaga aatggcagta tatctgcctg attcttgatt tgtttaatcg agaaatcatc
     1441 ggctattcat gcggggagaa gaaagatgcg acgttagtca aggaagcttt tgcaaagatt
     1501 ccgtactcgt tgaataatgt acaacttttt cacacggatc gtggaaaaga attcgataac
     1561 cagacgattg acgaaatttt atgggccttt aatatcacgc gttccttaag caaaaaaggc
     1621 tgcccctatg ataacgccgt tgcagaatcc acctataaat ctgtcaaagt agaattcgtt
     1681 catcaatacc gctttgagac attggctaat ttgcgattag agctatttga ttacgtccat
     1741 tggtggaata ccatccggct gcacggcacg ttaggctatg agacaccgat cagttttcga
     1801 cagcagagat tggcgcagcg aatccttgat aatgagctcg gatgtgatac cggtggcgag
     1861 gcaccttaat tatttgaatg agcttctgcc ttagagaatc agattcgagg aggctcgttg
     1921 tcaagggcag tcggaacacc agcagcaagc ttacgcaata ccttataatt tttgtcaaaa
     1981 aaagtgttgc cattccacat atatctttaa acagctattg ccagataaaa ataaatgata
     2041 ttatatatat aaatacttat ccgaaaaggg gaatgagaga gtcaaggttc ttggaatgtc
     2101 gaatagtcga gtaggtagat tttctatcta caaccttgtt gtttaaaaat gaaaaaaatt
     2161 ggaattagag aagttgcgaa agaggcaaat gtttctccta cgactgtgtc gagggtattg
     2221 aatgatcgtg gatatttgag tgaggaaaca aaaagaaaag tttttgatgc catgaaaaaa
     2281 ttagaatact atcctaatga actggcgaga gcacttttta aaaacgaaac atataccgta
     2341 ggtttgattt ttcctacaat tactaatcca tttcacgctg agttaattca agatatcgag
     2401 ttcgagcttt ccagcagggg atataaagtg ttgctgtgca atagcttaaa caatccaaca
     2461 aaagaaaaag agtacttgac gatgttgaga aagaatcagg ttgatggaat tatagtagga
     2521 acgcacaata aagatattgc ggattatgat attccagggc tacctattgt agcgattgat
     2581 agaagtcttg gtgaaaatac agttacagta tcttgtgata attacagggg tggggaatta
     2641 gctactcaac tacttgtaaa tcatggctgt cagaatatac tgtgtattcg gggagatagt
     2701 aagataaagc tacccgcgaa tagtaggact ttagcatata aagatgtttt gaataaattc
     2761 atgctggaac cgttagtcct agaagtacca tttgtaaaag aaatcaatga aaaaaatgaa
     2821 ataattaaga catatctgga aactcatcca aatattgacg gggtttttgc aggagatgat
     2881 ttattggctt ctttggcgat taacaactta ggaagttcgg gcaaaagagt tccaaaagag
     2941 gttaaagtga ttggatttga tggagctcaa caaacattga tttataatcc agaattatca
     3001 actattcaac agcctataga tcaaatatct aaagtagcag taaaaaaatt attgaatatg
     3061 attaagggcg aaaaggagac ggatgaactt tcacttccag taaagctcat aaaaaaaaat
     3121 acagcataag ttaaatcggc gaaaaattcc ttcgtcgatt taaaaatatt tttatgaaac
     3181 gggttgacat atgaaacggg ttgacatata ttagttgtga agataaaaac cttttcactg
     3241 gaggaagaaa atggagaaat tattaattaa aagcaataat catcaacttt actaccaacc
     3301 taaggatgtt tgggtcggag atgttatgcc ttacgggaaa gaaggaaaat tctttattta
     3361 tcatcaacgt gatactagaa atccagttcc ttttggagaa ccgtttggct gggctttagc
     3421 aactacgaaa gattttgttc attttgaaga ctttggggaa tctcttttaa gaggggatga
     3481 caatgagaga gaccagttta tctacgctgg ttctgtattt gaggcaaatg gaaaggttca
     3541 cgcattttat acagggtata atcgtgaatt cttagcgcag ggaaagacat ctcaagtgtt
     3601 gcttcatgct gagagtgatg attttgttca ttgggaaaaa tcagaaacag cattggagtt
     3661 agaaccacaa gaagggtatg atattcgaaa ctggagagat ccatttgtcg tttgggatga
     3721 tgaaaaagaa gaatatttgc taattttagg ggcgcgaaag ggtcaggata aacatctaca
     3781 aaccggacgt ttagtggaat ttacatcaaa agatttaact aattgggaat tcaaaggtga
     3841 tttttgggct ccgggattgt ataccatgtt tgaaatgcca gaacttttca agattggtga
     3901 ctggtggtat cttgtctatt cagaatactc agatggaaat aaaatactct atcgaatgag
     3961 tcaaagtttg gaaggtcctt ggattaagcc taaagatgaa gcttttgatg gacgggctta
     4021 ttatgcaggt agaacagctt atgatggaaa acgtcgagtc ctatttggat gggtaccaac
     4081 aaaagatcaa gaaaatgata aaaacaatta tttatgggga ggtactttgg ttcctcatga
     4141 agtctatcaa aaagaggatt tttcacttgg ggtaaaaccc atagacgaag tgtggaatgc
     4201 attcataaat ttacatgata ttgccgatgt agagatagaa acagcagaca caagagaaga
     4261 agtaacgctt gtcaaaaata gtggaacttt gtttaattta gaaacaacaa tttcatttga
     4321 tgagaatctt agtgactttt cactgagatt atataaaaac cttgagacag atgaatctta
     4381 cgaatatcgc ttcaatttag atgaggggcg tcttacattt gataaaaacc cttgttaccc
     4441 ttggtttcaa tatatggata aaggacttat tagacctata tctttaaagc ctaaccaatc
     4501 ttataaacta aaagtgattg ttgatgacaa tataatgaca ctttatattg atggaatcgc
     4561 tcttaacgct cgtgtacatg gttgttttgg tcaagcaatt actattacag caacaaatgg
     4621 atcaatgact gcaataaata ccaagttttc aaatgaaata aagaaataat aaacgtcaat
     4681 catgtggcag gtctgaaaat caaacatgac ctgtcacatt taaaagggag gataaatgaa
     4741 tggactacaa aaaaattgcc gaagatgtat tggaaaacgt tggaggaaaa gagaatatct
     4801 caagcgctac acactgcatt actcgtttac gtttaattct taaagacaaa agcaaagcga
     4861 atatggaaat gcttgaggaa atagaaggtg caaaaggtgt tcttttcaat agtggacagt
     4921 tacaagtaat tttcggaaca ggtataattg aaaaggttta caaagagttc gttgaattga
     4981 caggagctaa ggaagtatcc gtttctgaaa tgaaagacga aggagtcaat aattcaggaa
     5041 atagacttca acgagctttt aaagttttct cggatatctt tattccgatc attccagctt
     5101 ttattggggc agcaatggtt ttaggaattc gttcgttgat taccactcct ggaatgtttg
     5161 gaatggatgg ctcattcgct gatcacaatg tattcctaaa tgatttatcc aaatttctgg
     5221 gtattattgc aaccacattt gattatttac cagttttagt tatgtattct gcaacaaaac
     5281 gttttggcgg aaaccctatt ttaggattac ttgtaggatt tgttatgatt catccaggac
     5341 taaccaatcg gaatgatttc gtcatgggaa ctataaagcc tgattattgg catttatttg
     5401 gattaagcat tccagcagtt gcgtttcaag ggggtgtttt tccagcaatt ttgacttctt
     5461 ggtttttagc aaaagttgag aaaattacaa ataaatattc tcctcaagta cttgcttaca
     5521 ttttagtccc aactgtgaca attgttcttg ccaactttgc actattttta gtgtttggtc
     5581 cattggggaa ttttatcggt aatgttttag gtggaatcat tgatttccta tatatgaaga
     5641 tgggtgcctt tggtgctttt gtttttgcag gcgcgttaca accattagtt gtaacaggaa
     5701 cacaacatgc tattcaaggt attgaggcaa acttagtcgc aacaactgga ttcaattata
     5761 tccaacctat ttggtcagtt tcaattattg cccaaggtgg cggttgtatt ggtatgtatt
     5821 tactctttaa gaaaaaatct aaagatagag atattgcgat ttcaagcttt attccaacac
     5881 tggtaggtat tactgaaccg gctatctttg ctgttaattt gaaatattca atcgttcctt
     5941 ttgtttgctc gtttattggt gctggttttg gtggagcatt tatgaaaatt tttgatgtaa
     6001 aagccattgg acaaggattg actgtattgc caggtctgac tattgctaat ccagttcttc
     6061 cttatatatt tggtaatctc atagcattca ttttaccaat catttttatt ctcgcttatg
     6121 ataaatataa agtgtttatc cctgaagcta aacaccgtaa atttgcagaa aaaactgact
     6181 caaaaaaatt gccagttcaa gtaaagtgag atgagagcat gttaaatttt tttagaaaaa
     6241 atgaaataag cgttgtagca cctgcaaccg gtgtttttga aaaactggag aaattaagtg
     6301 atccagtctt ttcaaaagga atgatgggac aaggatttgc aattgatgca aaaaacgaaa
     6361 cgattgtatc tccaatcgat ggttttgttt caagtatttt tcctacaaaa catgccttag
     6421 gaataaaaac aaaatcagga attgagatac ttgttcatgt aggaatagat acagttgagc
     6481 taaatggtga gggatttgat attaaagttc aagaaggaca aaaagttaag gctggggata
     6541 ttcttatgac cattaatttt gtagtttttg aaaagaatca caagactaaa gatgtcattg
     6601 ttgtttttcc agactaccaa gacgaaattt tcattccgaa acttgaagaa gtaaaacaag
     6661 gacaagaatt gtttgttctt ggctaatgat aagtaattaa taatagaatc gaggaaaatg
     6721 tatggttttt ggggctattg aagctggtgg aacaaaattt gtttgtgcca ttggagataa
     6781 agatttgaac atatttgaaa aagtcagctt tcccacgact acacccaaag aaacaatgaa
     6841 tttagtttta gattttttta atcatcataa agatgattta gaaggtatcg gagtgggatc
     6901 gttcggtcct attgatattc attgtgaatc taaaacgtat ggcttcatta catcaacgcc
     6961 aaaacttgct tggcaggatt ttgattttat tggaagttta aaaaagcaac ttaatattcc
     7021 aactgcttgg acgactgatg taaatgcctc ttgctatggt gaatatgttt ctggaaatgg
     7081 cagaggcctt tcaagtgttg tttactatac cgtaggaacg ggcataggag gtggcgctat
     7141 ccaaaacgga aacttcattg aaggttttag tcatccagaa atgggacata tgcttgtaaa
     7201 acgacacaat aaagacaact tttctgggac atgtccattc cacggagact gtttagaggg
     7261 cttagctgct ggtccggcaa tcgaaagaag atcagggaaa aagggacaag aagtgactga
     7321 aaatgacgtt acttgggaaa tagaagctta ttacattgct cagtgtgcat ataatacaac
     7381 attgatgtta tcacctgatg tcattatatt tggtggcgga gtaatgaaac aaaaacactt
     7441 aaaaaagaaa gtacaagata agtttattga gttgatcaac ggatatgtcg ctattcccga
     7501 tgttgacaat tatattatga caccaaaatt gttagataat tctggtattg taggttgttt
     7561 agctttagca agagacgtta aagaaaaatc ttaatttgga aaaaagatcg tctcgactat
     7621 ttgtttggtt gatgcgatct ttttttcaat tagtaattga agggacgcct aaaagtgaaa
     7681 aaaaaaatga aagatgaagt attagagtat atcaaaaata atttaaagta ttacgatttt
     7741 tctgctcaag atattgcaat gaaattttgt attaaaagaa atgtcgcttc ccactatttg
     7801 aatcagttat tttcagatgg aaaattacta aagaatgatt ctgttagacc agtaatgttc
     7861 aagtataatc aacaaaagcc aaaagactgc ttttctaagt ttattggagc agacatatct
     7921 ttgaaatcca ctatagataa atgcaaagct actgtaatgt atccccctaa tggcctccca
     7981 ttaataataa aaggaaacag tggcgttgga aaaagttttt tagcaagttt gatctatcag
     8041 tatgctcttg atagaaaagt aattcataat gacgcaaaat ttgttgttgt gaattgtgca
     8101 gattatgcaa ataatccaga gctattgtca gcagttttat ttggatataa aaaaggagcc
     8161 tttactgggg ctgaaaaaga tactgcaggg atgctatctt ctgctaatgg aggatacctt
     8221 tttttagatg aggttcacaa tctaagtgca gaaaaccaag agaaattgtt tcttcttatg
     8281 gattctcaaa agtatcgtaa acttggtgaa agtgtgcaat gggagtatgc aaatgttcgc
     8341 cttatcttag ccacaacaga agataaaaat acctctttgt tagcaacttt tagaagacgc
     8401 atcccagctg aaatcactct gccagattac aataatagaa gtacatctga aaaaattcaa
     8461 ttattatttg aatttcttaa agatgaagca attacgattg attcaaaaat attttgttcg
     8521 attgaacttt ttactgatct tttatcgaaa acatttgaag gaaatgttgg agaattgagg
     8581 aatgaaatca agcttctttg tgcagaaggt tatttaggaa ataagagaaa gtcttcaatt
     8641 tatttagggg acaaactaga cagcggtttt tggatagatc cagaaattag tatagattta
     8701 aaaaaaatgt ttcttaaatc actttctgaa attgatttga ttgaactttt tcaaaatcac
     8761 atttcaattg aagaaagcat gagtcaatta agagatagaa tacttaaaga gtgtgcaggt
     8821 tcttctgagt ataattattt agaaagtgac gaatttatag cattaagaaa ctatgttgtt
     8881 aataaaatat caccaattat tgattcgaca ggtctatgtt tattagatga attcttggcg
     8941 gatatcagtc tatttataat gtttattgat gtaattgaag aaacatcaag aaattttttg
     9001 ctgtctaagt tcagaaaatt tcgtatgaaa aatgataaac aaaaattatt ggcaaatgag
     9061 atttggagta gtttagaagt cgaagaaagt aaacaagaac ttttgttgaa atggatactt
     9121 tttcttgtaa agaagttcta tgtgaaaata ccagaaaccc attgtctgat agttatgcac
     9181 ggtaaaataa cagcttctgc gatcgcaagg gaaacaaata agttgctgaa tacatatgtt
     9241 tatgaggctt tcgatatgcc aattgaaggt gagactagtg acttaattaa cctaatcaac
     9301 aatttttgta aaagtataga tacgacgaat ggactcattc tgctagttga tatgggatca
     9361 ttggaacaaa tgtatgaaaa aattgaatcc aatgtaatag gtgatttggt aattttaaac
     9421 aatgtttcta cagctttagc gattgaatgt gggattcaag tttgccaaaa aaagccaatt
     9481 agtcattttt accagatgga ttttgattcg tttcaagtta aagttcagta ttataaagga
     9541 ttatcacaaa agaaaaatgt aatagtatct tgtctctctg gtgaggggat ttctgaaaaa
     9601 gttaaggata ttttaaaacg gtatctcgat aatcaagtgg agattttgat ttttgatttt
     9661 aacgcattaa agaaaatagc aaaggaaaaa gatactatga tttttaagaa tacaatttgt
     9721 gttctttcaa ccactgaatt ttacattcaa ggaatcgatt gtttaaattt agaaaatcta
     9781 attaatggga atcaaacctt agaaaaatta aataagtata tggactcgga tagctgtgag
     9841 ttctgcttaa atgaattagt taaactcttt actattgaag gcgctagtac taagcttcgc
     9901 tttttagatt ccaaaaaagt attcaatgag atagaaaaag tactatattt gtatgaaaaa
     9961 tattacgatg tcaaaatccc aagttttctg agaattaatc tatttctaca tctttctgga
    10021 atgattgaac gaattatgat tggtgatgga ataacaaatg aacatttgaa agagggagcg
    10081 caactatttg atactttttt aaatgtatca aaagattttt tttcagaaat caaagatatt
    10141 tactgtattg aaatacccaa aggagagtat gagttaatct atacaatttt tgagcagact
    10201 attttctaaa tagcatacta gcgtctaaaa aaatgaaagg gtgtccaaag tatatttaga
    10261 cgctcattta attaatattt actgtatttt cccgctttat tggataatgt tagttggcac
    10321 agtaattgct tatataaaag tataagaaga aaggagagaa caaaatggcg ttttggcaaa
    10381 catttttagt tgtgttgtat ggatttttca ttaactggga taaggaaggg cccaaaatcg
    10441 gaacctccca acctgtcgtt gcaggtttct tgaccggact tattatggga gatattcaaa
    10501 ctggacttta tatcggtggg actttgcaat taatgacact aggtattact agttttggtg
    10561 gtgcatcagt accggattat caaacagcag cattgatcgg tacttattta gcaatctcaa
    10621 ctaaccagga tgcttctata ggaataactt tagcaatacc ggtagcaatg ttaattgtac
    10681 aactagatgt tctaaagtgg tcaacaaaca ttttttttca acaaaaggca gaaaaattcg
    10741 cacaaaaaga agattatcaa aaattagagt ggatgcaata tttggcatca attaatacaa
    10801 gctttgtatc tggaattcct gttttgttaa ctgtgatatt aggcccaacg ttagtgggaa
    10861 gtgcaatcaa ttatattcct gaatgggtta ccggtggttt aaaggtagca gggggactat
    10921 tacctgctgt tgggatagga atgctactta aatatttacc tacaaaagat tactttgcct
    10981 atttgttagt tggtttcgtt ttggcagttt atttccaaat accaatttta gggatttcgt
    11041 tacttggcgc tgcaatcgca ctaatcgtat ataaaaatag atcaaataat actcaagtag
    11101 caccagctgc aacaggaggt tttgacgaag atgaataaga aactgacaaa aatcgacaaa
    11161 caagtaattt tccggtggat tctcaatggt gcaacttcat taaattatga aaaaatggaa
    11221 gggttgggtt atgcgtattc tatgcttcct tttatcaagg acacatataa agatgatcct
    11281 gaaggaatga aacaatccgt tttagctcac ttacaatttt ttaacactac gccgtataca
    11341 gcgccttaca ttattggtat gaatgttgga attgaaaaag aagaaaaaaa taagtctttg
    11401 gaagcagtaa gtgctctgaa aacaggtcta atgggtcctt tagcgggaat tggagatagt
    11461 gtatttgtag ttattccatg gactattttt ggagcaattg cagctaatat ggcattggaa
    11521 ggtagttttg ttggaattat tttgtggata ctggcatcaa ttttattaag gattactgct
    11581 tatcctttat accttgctgg gaaagaatcg ggtgcgagcc tactaactca aattgaaaca
    11641 aaattaaagt ctattactga tgcagtatca attttagggt taatggtggt cggtgcactc
    11701 attgccacag ttgtaaaagt gaatgttggc tttgaattta aacaaggcga cttaacaatg
    11761 accggaaatg aaatctttga tcaagttatg cctggtttat tacctgcatg tgtagtagca
    11821 attgtgtatt atttgttagg acgaaaggtt aaaccagttt atgtaattct attaatctta
    11881 gtagtttcta ttgcgttaag agcgttaaat attttgaagt aggtgttgaa tatggatgaa
    11941 aaaatgaaag gtattataca tgttcgtatt gatgatcgaa tgattcatgg acaagtagca
    12001 acacaatgga caaatcaatt gagtgctaac agaattatgg ttattaataa tgaagtagcc
    12061 aatgatgacg taaaaaaagc tgtagttcgc ctcgctgcac cacctaatgt tagaacttca
    12121 ataattacaa gggaagtagc agttaaaaat attctttctg gtaaatatga aggacaaaaa
    12181 gttcttattg ttgcagtttc tccaatcgat gttaaatttt tgattgataa cggtttacca
    12241 ataacaagtg taaacgtagg aaatttatca agaaggaatg gaacagaacg catccgtccg
    12301 acgataaata ttactgaaga agagagagag gcttttaaag aactaattag tgatggagtt
    12361 gaagtgacag tgatacaaac tcctagagat tcaaaagaat ttttaaagga ttttatttat
    12421 taagaacaga ggatgagtgg atggagttta taattacatc tcatggaaat attgccattg
    12481 gatttgtaga tacattaaaa atgttttttg gcgataattt aaattgtcat gtggtaacac
    12541 ttggagatgc aggcattgaa gaattccgtg aaaacattga aaaaatagtt tctcaagtaa
    12601 aaaatcaaga agttatggta tttgctgact tgattggtgg aacacctttc aacgaatttg
    12661 ctaaaagaag tgaagtattt caaaatgggt ttcaacttct tggtggcatg aatttaggaa
    12721 ttttgattga ggctgtcaat ttgagattac aaggaaaaaa aagcgatgat atcatcgaaa
    12781 atctaagaaa tatgaataca attgcgtgtc ttgcagatat gcaaagacat actaacgaag
    12841 aggatgagtg ataaatgcaa aaaatttttt acaagcctaa agatttttgg tttggggatt
    12901 gcatgccttt ttataaagat ggaacttttt atctatttca tcaacgagat acgagaaatc
    12961 ctaaaccttt aactgatcct tttgggtggt cactagttac aaccactgat tttgtgaaat
    13021 ttgaagaata cggggaagta attcatcgtg gtggcaacga tgacattgat caatacattt
    13081 acgctggaag tattgtccaa ggaatcaatg agtatttagc tttgtatact ggacataatc
    13141 gcaaagctaa gttggatgga aaaacttcag aagtgttaat gattgcaagt tctaaagatt
    13201 taattcattg gaaaaaggat ggcgaagcag caaaacttgt tcctcaagaa ggatatgata
    13261 agaatgattg gcgcgatcct tttgcaattt ttgatcaaga tcatggaaaa tacgttttga
    13321 tactaggagc tagaaaacca gcaagtaaga aaaagccgac ggggagattg gtctattttg
    13381 aatccgaaga tttaaaagat tggaatttca aaggagacta ctgggctaat aatgaattta
    13441 acatgattga aatgcctgat atatttgaaa ttgatggaac gtggtattta cttttttcag
    13501 agtattgtga agataaaaag actaaatatc gagtcggagc aggattattt tcagattgga
    13561 aatcaccagt agacgaagct ttcgacggaa aagcctatta tgcagcaaga acagtcggaa
    13621 ctaatgaaac tggccgtttt ctaactggct gggtggcaac aaaagaaggg gagaacgatc
    13681 aaaataactg ggattggggc ggtgcacttg ttccacatca aattattcaa aagacagaca
    13741 aaactcttgg tgttaaactg cctgaaaaaa taattgagaa ttttaaagaa atcactccat
    13801 tttcaagttt taagttagag aaagaatttg gaaaaaaaga aagagtaatt gttcctaaaa
    13861 tcgaagatta ttttctattt gaaacaactt tgactattac atcgttaaca aaggagtttg
    13921 ctttaaaatt atttgtagat agtgatactc aagaaggata cgaatttcgt atttcattga
    13981 ttaataatca gcttactttc ggaaaaactc cgaactatcc ttggcctcaa atgtttgata
    14041 aggggctgca acgaccacta tttttggaaa taggcaaaga atatgaacta aagttaattg
    14101 ttgatgatac gatagctgtt ttatatattg ataatgttgc tttaagtact agaatgtatg
    14161 ataaagtcgg aacagcatta agtatgactg ttacggaggg agcaatcaag gtagataaac
    14221 tagactattc aataaattat gaatgtggat tttaaaacaa ttgaagggat gatgtaagtg
    14281 aaattaaatg gtttacaaca tattggaatt ccaacaaaag attttggagc ttcaaaaatc
    14341 ttttatgaat ccttagggtt tagtctgatt aatgaagaaa tgaatgtggg aagtaaagtc
    14401 ggctttttcg agttgaatgg aacgataata gaaatatggg aagataaaac tactgagtgt
    14461 agaggagcca ttgatcatct agcattagat acggatgaca tagatgaggt attcaattca
    14521 attcaattca agatttagga tatgaattat tagataaaaa tattatggaa ttaccgttct
    14581 gggaaaatgg aatccgttac tttaatttct acggaccaaa tcatgagatt atagagataa
    14641 gccaaatgaa gaaataaata gataacgaag tgctctaagt tggtatgaac gaaaagtact
    14701 tcattttttg gtatcagcat ttcaatagtt taatgagaat ttactatttc aaattaattc
    14761 aaagtgacag tattttttac ttatttggtt tcaaattttg taatccttcc tatttattgg
    14821 aatacactcc acattttgta tacaagtcat atcatgtttt agcaaagctc ttaccaagca
    14881 aagttataat gatgttttcc aaagtttgat ctttcgatgg aggctaacac atattttttg
    14941 caggacagcc aaaat
//
LOCUS       DACGLH010000067        14549 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.072, whole genome shotgun sequence.
ACCESSION   DACGLH010000067 DACGLH010000000
VERSION     DACGLH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.072"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            4..816
                     /locus_tag="IXT21_002168"
     CDS             4..816
                     /locus_tag="IXT21_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ4869550.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IXT21_002169"
     CDS             813..1613
                     /locus_tag="IXT21_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ4869551.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IXT21_002170"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IXT21_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ4869552.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IXT21_002171"
     CDS             3110..4819
                     /locus_tag="IXT21_002171"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ4869553.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IXT21_002172"
     CDS             5031..9389
                     /locus_tag="IXT21_002172"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ4869554.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IXT21_002173"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IXT21_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ4869555.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IXT21_002174"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IXT21_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ4869556.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IXT21_002175"
     CDS             11227..11520
                     /locus_tag="IXT21_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ4869557.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IXT21_002176"
     CDS             11517..11828
                     /locus_tag="IXT21_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ4869558.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IXT21_002177"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IXT21_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ4869559.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IXT21_002178"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IXT21_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ4869560.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2581 c         3236 g         3773 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag cagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtggtc acagcagccg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcagtaata ggagtttacg agctgtttcg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtattg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattta atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg ttcgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcctgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccggataaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcgt attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct acaccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gattatcgga gacggtggcg caatgggcgg
     3721 gaaagactca aaagaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgcggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtactttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaat ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tcttcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaactg
     4501 gccaaaagga attgcgccat ttgatttgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaaac ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gtgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctgcatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttag ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DACGLH010000068        14440 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.073, whole genome shotgun sequence.
ACCESSION   DACGLH010000068 DACGLH010000000
VERSION     DACGLH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.073"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(58..855)
                     /locus_tag="IXT21_002179"
     CDS             complement(58..855)
                     /locus_tag="IXT21_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ4869561.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSVPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVIKAISQQLKDVTSIFKLPKAVGKLLGSIQTNLPESVLLDCGMDFL
                     KDNNKKIDTLSVPVDGSWDFNDNTPSGSVLELDLTKNQEAIKKFLNN"
     gene            complement(947..2110)
                     /locus_tag="IXT21_002180"
     CDS             complement(947..2110)
                     /locus_tag="IXT21_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ4869562.1"
                     /translation="MKKRKKIDWWILGPYLTLSMIGLLEVYSASSYRLLQADENTKSL
                     LLRQLIFIFLSWSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGA
                     QRWISLFGIQFQPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPK
                     IAGALMILSIAWVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNH
                     AYERIATLRDSFIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIF
                     SIITEELGLIGALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVG
                     SIAGLMPMTGVPLPFVSYGGTSYLILSLGIGITLNISSKIQAEELPLYRPEKQ"
     gene            complement(2627..2833)
                     /locus_tag="IXT21_002181"
     CDS             complement(2627..2833)
                     /locus_tag="IXT21_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869563.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2849..3463)
                     /locus_tag="IXT21_002182"
     CDS             complement(2849..3463)
                     /locus_tag="IXT21_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ4869564.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETKEAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(3610..4293)
                     /locus_tag="IXT21_002183"
     CDS             complement(3610..4293)
                     /locus_tag="IXT21_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ4869565.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RSNTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(4402..4860)
                     /locus_tag="IXT21_002184"
     CDS             complement(4402..4860)
                     /locus_tag="IXT21_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ4869566.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4944..6707)
                     /locus_tag="IXT21_002185"
     CDS             complement(4944..6707)
                     /locus_tag="IXT21_002185"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ4869567.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6928..8388)
                     /locus_tag="IXT21_002186"
     CDS             complement(6928..8388)
                     /locus_tag="IXT21_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869568.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(8596..9522)
                     /gene="glsA"
                     /locus_tag="IXT21_002187"
     CDS             complement(8596..9522)
                     /gene="glsA"
                     /locus_tag="IXT21_002187"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ4869569.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(9545..10972)
                     /locus_tag="IXT21_002188"
     CDS             complement(9545..10972)
                     /locus_tag="IXT21_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4869570.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(11003..12217)
                     /locus_tag="IXT21_002189"
     CDS             complement(11003..12217)
                     /locus_tag="IXT21_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ4869571.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(12558..12800)
                     /locus_tag="IXT21_002190"
     CDS             complement(12558..12800)
                     /locus_tag="IXT21_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4869572.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(12832..13389)
                     /locus_tag="IXT21_002191"
     CDS             complement(12832..13389)
                     /locus_tag="IXT21_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4869573.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(13402..13590)
                     /locus_tag="IXT21_002192"
     CDS             complement(13402..13590)
                     /locus_tag="IXT21_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ4869574.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(13603..14169)
                     /gene="amaP"
                     /locus_tag="IXT21_002193"
     CDS             complement(13603..14169)
                     /gene="amaP"
                     /locus_tag="IXT21_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ4869575.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4308 a         2968 c         2365 g         4799 t
ORIGIN      
        1 aaaagaagtc agaacataac atagattgtt atttctaact tcttttattt tctttactta
       61 attattcaga aattttttga ttgcttcttg gtttttggtc aaatctaatt ccagaacact
      121 tccggaaggc gtattgtcgt tgaagtccca actgccgtct actggtacgg ataatgtatc
      181 gatcttttta ttattgtctt tgaggaaatc cataccacaa tccagaagca cactttctgg
      241 aaggttcgtt tggatactac caagtaattt cccaaccgct ttgggtagct tgaatattga
      301 agttacgtct ttcagctgct gagagatagc tttgatcact tgctctcctt tgtttatata
      361 tacgccatct aaatctagag atttttctgc atcgatcttg actccaccag gaaacagttc
      421 attaacgcaa tcgataaaat cttgaaatgt gatacttgca taatagggag cttcaaattt
      481 gaaattttcc tttaatgttt gatttaataa atcaactccc ccataagagt aagctgcatt
      541 tatcttattc attccgtaac ctggaatatc gacatatgaa tctctcatga tagagatcag
      601 ttttgcttgt tgtgttttct ggttgtattg tgcaaccaga agtgaatccg agcgaccgtt
      661 ttcgtctttc gaacgagcat ctgaaccaat caaagccaca tttagtgtgg tagggacact
      721 tttcggctct acctttttca cagttgaact ctgttttgca gacgaaaatc catggataaa
      781 ctcaatcgaa aaatagccaa ctactaatgt aagcagtaac aaaatagata atatgatttt
      841 ttgagatact ttcattgcga attcctcttt ctaaaagaac ctctcgcttg aaaaataaaa
      901 gattgtactt tatgataatt caaactgaga gtttctcagt ttctctttat tgtttttcag
      961 gtcgatagag cggcaactct tcggcctgta tctttgaaga aatattcaac gtgattccga
     1021 ttcctaaaga aaggatcaga taacttgttc ctccgtagct aacaaatggc aatggcacac
     1081 cagtcattgg catcagcccc gcgatacttc ccacgttcat tatcgtctga acgaatagca
     1141 aagttccgaa tcctaaaaga aacaatccag cttgctgatt tttacagcga ctgcttaaac
     1201 aaaagattct catgcataat gagaacaata agaaaagcac acacaatgca ccgattagcc
     1261 caagctcttc tgtaataatc gaaaaaataa aatctgtttc tgtctctgga agataccctt
     1321 tttttgtgat actatttcct aatccccgcc caaatatgcc accattatat agtgcataaa
     1381 acgaatgggt catttgatag cccgcaccat gactatcgat aaaagaatct cttaaagtcg
     1441 cgatcctttc ataagcatga ttaaacatct gtggaagcca acctttattt cctaaatata
     1501 ataccccgcc tgctgctcca atcagcaatg cagaaaaagt aacgattaga tagatccctt
     1561 ttttaaatgg aaccgccgct gcccaaaata tgacccacgc aatcgaaagg atcatcaatg
     1621 ctccagcaat ctttggctga aataaaatca gaaaagttat acctactgta atcaggaatg
     1681 gtttttttag atcttttggg ctactatttc cgtcacgaaa aaaccagctt aaataaaaaa
     1741 tcaaaaaaag atttgccagt tcagaaggct ggaattgaat gccaaacaga gagatccaac
     1801 gttgtgcgcc gttgacagtg acaccgaata tccctattct tactaatact aaaaagaaaa
     1861 tcgataaggc taaaccgtat cctgctattt tagggtgaag taaatagtgt agtttgactg
     1921 aacgagctaa gaagatcacg ctccaactca aaaatatgaa aattagttga cgcaataata
     1981 gactttttgt attttcatcg gcttgcaata aacgataaga actggcactg tatacttcta
     2041 aaagaccgat catggacaaa gtcaaataag gacccaaaat ccaccaatcg atcttttttc
     2101 tttttttcac cgcatctcct cctcacacag caatccactt gtcaggtgag gagcgagttt
     2161 gtctcatcaa ataagtcagt cacaaaaata taaagaaaag gtcagaaacc ttttcgaaca
     2221 agaattctga cctagcatca tctcatcgtc cgagttttag cactatacaa cgtaaagaaa
     2281 acattcttag ctattggaaa aagccttaat ccgacagttt ctgttttacc cattggcgac
     2341 tttcgacgtt tctgggcaac agcctatgtt tgtataggag cctcacctaa caaggttatt
     2401 actatattct atttacgagt tcatgataga cttttcctaa tgattaggca atggaatttg
     2461 aaatgaaata gtgaaattta acaatcttta atatttttta gaaaaaaata acaaataagt
     2521 tgaaagaatc ccgttgcaaa ttctttcagt tgcaccagga ttctttgata attttttagt
     2581 tttatgtgtt tttttatatg tttttttcac ttttcggaat aaatatttat ttgttaaagt
     2641 aattaaacaa tccgctaatc gtttgatctg gcatttctat ttccttacca tctaaagtaa
     2701 cagtagtaac gacaaaatgg tcttgcaatg gcggaacttc taagtactgc ccttgcagaa
     2761 aatcattcac tgtttcatat tcttttttca tttcttttga atctgtcgat ggtttatagc
     2821 ttatttttaa cataactagt gctccttttt attcgggacg tgcaatcgtg aaaatttctc
     2881 ctattgctgc ataatcattt cctgccaaac ggcttacagc attcaatttt cttggatcga
     2941 tccttcctgt cttatcatca tagactgttt catttaaatg atatttctgt acttctagca
     3001 aaaataaatc agctgttact ttatttttat tccgtatctc gacatgttca tataaggatg
     3061 tttcaaaacg aacttttgct tctgtcaatg ctggtactga aatgattact gaatcagtta
     3121 aagtcaagtc agtcaggtac agttcgcttt cctcattagg cagattggct gctgttttat
     3181 ttacttcttc tacgttgtcc tcatcaagga tatggataac agcttctttt gtctcaagaa
     3241 tattctttgc tgtgtctttt actgcatctt gttttctctg gatcgccaag gataagatgg
     3301 gtcgattcga actgacgata ttgaagtaac tgaagggtgc gatattcact atatcttttg
     3361 ctgatttagt cgttactact gctactggtc tagggatgac tgaaccaatc agcaacttgt
     3421 aattttcacg ttctgtcaat tctgaaggac tccgttcaaa catggtttcg ccttctttct
     3481 ataaaattcg ttaaaaagca gatcttgaag acaaaatgaa aagtaatgct tttttcatct
     3541 tcttagaatc tgctttaaac tatctatcaa tttttctttt ttgtcaagaa tctgtgccgt
     3601 gcgtgttttt tagttatttg ctttacttgc ggcttcgaca tatcttttag tgatcaattg
     3661 cggacgggtg ataatagatc caactacgac tgaaaaagca cctaattcat atgctttctc
     3721 tacttgttca ggagagtcag tatgtccttc cataattact gggatatcga cttgttctat
     3781 gatttgattc aataaatcaa aatcaggtgt ctgcgtcttt cgtgaatatt ctgtatagcc
     3841 tgctaaagtc gtagacaaaa gatcaaaacc atcttcttgc gcttgaagcg cttcctcaaa
     3901 agtcgaaata tctgccatag ctaaccgacc gtgttcgtga atcatttgga caagttcagc
     3961 agttgttttt tctgtcggac gtttacgaga agttgcatcc agtgcaatga tttctgcttg
     4021 cgtctccaaa agttcttcca cttctttttc tgtcgcagta ataaacactt ctgacccatc
     4081 gtaatctctt ttgataatcc ctattaccgg taaatcaacg attcgcttga tttcattgat
     4141 atcttctgtc gtatttgatc gaataccaac tgctccgcct tcttttgcag ccaaagccat
     4201 tctccccata ataaaagaac tgtgtagcgg ctcgttttcc aaagcttgac aagaaacgac
     4261 gactccgcct ttgatttgtt ccagtgtatc cacaatatct cctccagtaa tgttatataa
     4321 aaatacagct tcatataaaa atgtaaccct tttctattca ggagtcaata atgagctatt
     4381 tctttcagca gacctttctt actaaaagaa aattacttca aattcttcgt atacgaccag
     4441 cttctcttct gtaaattcta tgttcaacgc ttctttataa gcatgtttaa aaaactcagc
     4501 atggactttc cgccaataag ctaatgtccg atccccttct ccttctttcc aagcatgctc
     4561 tttcgtgact tcactaaaag gcaagatggt cacatttata tttttgatga tacaaactgg
     4621 ttcatttttt ccattcagga ccacgtctaa ttgaccaact tttggtaaag gctcattctc
     4681 ttgtttatac agatcgtaac cacttgatgt accagtcttt atctttttta caactagttc
     4741 gctcaactcg tctgccattt tttctgtatc cccaaatgaa aagaaaccta atttttctga
     4801 ttgataacta ggatattttt cttggaactt tttgacatat tcttctactt gctgatacat
     4861 aacatccacc cctttgcttt ttataatgaa aaaaccatcc agatactagt ataaacaatt
     4921 atctggatgg tgagcataat gaattattga atattcaaga cacgcagact atcttcaacg
     4981 atttgttttg gtgttaaatg ataacgttca taaagctgcc ctacaggaac gctatccgtg
     5041 aattctttct ctgcaccaaa atttaatacg ttcattgaag aatcaccata ataacgactg
     5101 atattttcgc caaatcctcc tgacaaacta ccatcttcta atgtgatgac taattcgtgt
     5161 gtttctttta atttatctaa tgtttctacg tcattttctg tgatcgttcc tggattgatc
     5221 aacgttgctt cgattccttt ttttctaaaa gcttttactg tttcttttcc taattcaaga
     5281 aaaccgccta aagctaaaat tgctactttt cctccattat ttactgtttg ataggctgga
     5341 atactataat catttgcgat agctgtacca tgttctacac catgtgtagg aattcggatt
     5401 gctacaggct gcgctgtttg ttcgaacgcc caatctagca tagagatcat atcctctttt
     5461 gttgctggag caagatattt gatgttcggg atacttgata agtaaggaat gtcaaatgtt
     5521 ccttggtgtg ttcggtcact agctgagatc gttccgccac cgactaaaat aatcactgga
     5581 ttctcgttga tcgctaaatc atgtgataat tgatcatatg cccgttgcaa gaaagtactt
     5641 gattggaaga cgactggacg tactccctga gaagccaaag cagaagcgaa agtgatagag
     5701 tgctgttcag cgatccccac gtcaaaataa tgatctggat gtttttcttg gaatgttttc
     5761 agtccaaagg cgcctggtat cgctgcattg actgcggcaa taggagctgt acttgtttgc
     5821 attttcttat ccaaataatc catgatgatc atattaaatg tttctccaga agttacttgc
     5881 tttgcctccc cggtggacag atcaaatggt acatgccaat gatatttcat tttattatcg
     5941 attgccggct gataaccgcg acctttttcc gtatggacgt gaagtacaat cggatgatca
     6001 atatctttta cttcttcaaa taaacggatc accgtttcca gatcgtttcc attatccacg
     6061 taacgataat caaatcccat tgctttaaat aggttattgc tgcttttacc gttggtttga
     6121 cgaagctcag ctaagttttt atataaactg ccatgattct catcaataga catctcatta
     6181 tcgtttacta atacgattag attggattgt aaaacaccac cattgttcaa tccttcaaaa
     6241 gctaagccac cacttaggga gccgtcaccg ataaatgcta ccacatttcc tgattctttc
     6301 ttcagatctc tcgctttagc taaacctgtt gctaagctga tagatgtaga tgtgtgcccc
     6361 actgtaaaat agtcatgttt actttcatgt tggttggaat atccgctcac ttcacggtaa
     6421 tgtccttttt caaaaccatt ttttcgacca gttaaaagct tatgaccata agactgatgg
     6481 gaaacatccc aaacgatttt atcctttggt gaatcaaata cataatgaaa cgcaatcgtt
     6541 aattctacta cacctagatt cggaccaacg tgcccaccta catcgctgtc cttttgcaag
     6601 attttttggc gcatctcttc tgccaactgt ttcatttcct caacagataa ttttttgaca
     6661 tctgcggggc tattgattgc atccaataca tgtaatactg tactcataat acattctctc
     6721 ctccgtacta tcttctttta atgaaaatag atttgttaca ctttatctat tctactactt
     6781 agagttgact ctaagtcaat agcattttaa aaaaatttca gagaaagctt gttatataag
     6841 tagatcttcg cacgaatcag gactcaaata aaaaccttct acagacaatt aaaaagcttt
     6901 gtagaaggtc tcctaattaa ggtctcatca agaaatatgt ttttttaata ggtgaagttc
     6961 ctgagcaata tgatacaaat ggatgtttgc accttttaat gagattggat agatctttgt
     7021 cttttcagtt tctaatccat tttgatagct agcagcatcg cacaaaacca ctcttttttt
     7081 cgcacataga cattcatcag gcaattcttc taatgaacga aataaaatag gatttatttc
     7141 agcgatgcct tcttccgcat acaaatgaaa ttgggttcga taaaaaaatt gtttgactga
     7201 actttctgaa acaacaaaaa tttctatttc ctgtctcccc gcttgttcac tgttgatcaa
     7261 agaaagactt ttgacgaatt ttcgaatgaa attttcattc catcttagtt ggatttgatt
     7321 tttctttttc cactcttcca atatttctac aatttttccg tacagaagtt gttctttttc
     7381 tgatagaaga tagatccttt ccggtaaaaa tacttgtcca tctgcccaca acgtacggac
     7441 aaattgtaaa aaggattgct tgaaaataaa attttctgca tcgtcgattt cagcgatact
     7501 aataatctgt aaaatcaatt cattcagtga taggtcattg agaacaaaat ttttataaac
     7561 aacttcgaaa tcttttctta acagctccat gttctcattg gtataatttc tactgttgaa
     7621 caaagaaaaa atataataga catcttcttc ttgtaagtct agaattcctt tttgctcttc
     7681 caatccttga cagactaatt gaaataacgg cttcgtttct gcgcttttct tatcttcttc
     7741 tttcagtcca aggggaaatg cctttcttcc gattgcgatc tctattcctc gtatgataaa
     7801 ggacttttca tcttccgaat atcttctttt aaaaaaatga gtctctacat aatcaatcaa
     7861 ctcgttacaa gcttcttcta catacggatt tttattttca tagccttccc aattgacata
     7921 acgtactaaa gctagcaaga tgttccgcga gtcaaattct gggataatga tttctttatt
     7981 tttcaaataa tcattttctt taaaaaaatc caatacgatt tttttgattg tatatacttt
     8041 gctcaaagaa ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata
     8101 ataacttaaa aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag
     8161 agcaatacca tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat
     8221 acaatattct agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac
     8281 ccttagagaa atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca
     8341 cagatacact tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg
     8401 cctccagttt ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt
     8461 tttatttaga aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac
     8521 aagctcacaa tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc
     8581 ctctttcaag aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa
     8641 caattggata ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat
     8701 gccgtaacga tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac
     8761 accaacatgg acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga
     8821 tttgacgatt gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc
     8881 tttatttgcc agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca
     8941 ttgtctgaaa taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt
     9001 catatagtaa gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc
     9061 ttcattcaat gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc
     9121 aaatttttca tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt
     9181 aacaaaagga ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa
     9241 ggcaaaaccg gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt
     9301 gattgctaat aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc
     9361 gtctcctccc tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt
     9421 gggatccact tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg
     9481 gaaatttttt ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca
     9541 ttttttattt ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa
     9601 ctaagacgat aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg
     9661 cgatactagc aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta
     9721 aacaagcaac gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac
     9781 tgtcatcttt aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt
     9841 agattacaac agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta
     9901 atgtaaagac ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag
     9961 gaacaccgtg tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta
    10021 cgtgaagtgc ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac
    10081 aaacaagaat cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata
    10141 ggaattctaa cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag
    10201 tagcgacggt caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca
    10261 ttgctaatgg ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt
    10321 ctacgcccat atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa
    10381 aatcaggaat caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat
    10441 aatggatccc taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga
    10501 tccgagcaat caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga
    10561 aaataatcaa taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg
    10621 ttgcatagga taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct
    10681 caaaccattg gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc
    10741 aagtatagac accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg
    10801 ccacagggat aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg
    10861 aggtcgcgaa tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa
    10921 agccaaagaa cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc
    10981 ctcttttcta tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc
    11041 cttcttcttg ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa
    11101 tcactgttgt cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc
    11161 ctaagaagta ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta
    11221 tcagaatcgt acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat
    11281 cccattgttt ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc
    11341 caatcagcat ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc
    11401 cagcaacgga tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa
    11461 tgatcagcca agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga
    11521 ctgcttgttc atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa
    11581 gcagtcctgt accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag
    11641 gaatctttct ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt
    11701 ggatgactgc tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc
    11761 atatccagtg gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat
    11821 agccttttaa attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga
    11881 taaacatcag ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt
    11941 tggggaagct tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc
    12001 cgatatcccg tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc
    12061 caacggagag aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc
    12121 cgtaaaaaaa agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga
    12181 atgctgtatc tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat
    12241 tacgagtata aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc
    12301 atttataaaa gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca
    12361 cacaaataaa gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct
    12421 ttttttattt ttaaagatat tttttcataa ggaataaaga atacttcata taaaatatat
    12481 aaaaaagaac cgcagcgagt gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa
    12541 gtttttcttc aatttgctta taatttacgt gcaaagaatg aaacgactaa tactaaaatg
    12601 attgcaccga tcaatgatgg aatgatagcc attcctgcaa ttgttggtcc ccaagttcct
    12661 agtaaagctt gtccaattgc tgaacctact aaaccagcaa tgatgtttgc gatgatcccc
    12721 attgatgatc ctttattcgt gattgctcct gcaattgctc caatgatacc gccgacgatt
    12781 aatgcccaaa taaatcccat tttaaattcc tcctaactga cttcttactt cttattgtac
    12841 acgtggttcg ttattttctt tgactttttg tgcacctttg cctacagcag attttgcttt
    12901 atctgtttgg cgtgaagcaa attgtcctgt ggattttgct gcttctgtta ctttgtcttg
    12961 tactgtttct tgatcttttt ggtattcttc ttgtgttttg atgtctgcaa cattcacatt
    13021 cacttcgata acatctaaat gtgtcatatt gtttacttca gtactgatca attctttgat
    13081 ttggttgtaa atatcttcga tgtctttacc gtattctacg atcacatcta gatccacagc
    13141 tacttgtttc ttacctactt ctgtattgat acctgctgtt acgttatctg tgttgactaa
    13201 tttttcagcc atatttgaga aaaatccgcc atctaatgtc aatagaccat ctacatcttt
    13261 caatgcataa ccgatgattt tttggatgac tttgtcttca taagtcaatt caccattcat
    13321 catgcggttt tctccttttt catttgccaa aactgatcca tttgttgtat tcatgttttt
    13381 gttttccatc tttgtttcct cctatgattt aaaaaattga tcaactattc ctgactgttt
    13441 caaataaaaa ccgattcctg cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa
    13501 aaatccaatc gttaaccata aaacagctag cagtagtcca agacccccaa agataatagg
    13561 gagcttgtat gctgaaaata gttctttcat tatttggccc tcctattcca ctcgagagtg
    13621 cttgtagtct cttgtatctt cttgttcttc ataaccgctg tatgtgactg ccactttgac
    13681 cgtttctttt gttccgagta ctttagttac ttcttcttcg atatcttgca tcaatacacc
    13741 tgttttccct actaatgaag aagttcgttt caagtctccg ttgacatgga tatggattcg
    13801 atttttcgtt gaacgtatcc gtactttagg tgaatgaatg aactcttctt cgtgtaaatg
    13861 ggaacgtacc aacccttcga tcgctttttt gtcgattgtt agtttgccat cttcccgttt
    13921 cataacgaaa gttcctcttg actttggata gaaaagaacg actagcaaga tgataatagc
    13981 aagaacggca aatactacag ctgcccagaa aatatattgt tgcatgtagt aattcgttaa
    14041 agtaaagaag cgaaatcgtt ccaattgttc aggcataata tacggcgcat tgatcaacgc
    14101 aatcgctaga aatactgaaa gtagcagtaa actcatgata acaccgatta tttttaaccc
    14161 cttattcatt ttccacctcc tataaactta tttttaccct gtaaataact ctaccgacaa
    14221 tcacagtata gatagcgaac agttagcata gcatcagtaa ataaagcgct accaaacatt
    14281 gctggatcat aacggcgttt tattgccagt ctgatactga aataacaatt gcttacttat
    14341 tctctattta cttttgattg ctggctatac ttaaaggata ttattcaaga ttaaaaattg
    14401 caaagattat gctcagttat tattacaaga ttctatttat
//
LOCUS       DACGLH010000069        14119 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.074, whole genome shotgun sequence.
ACCESSION   DACGLH010000069 DACGLH010000000
VERSION     DACGLH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.074"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..596)
                     /locus_tag="IXT21_002194"
     CDS             complement(<1..596)
                     /locus_tag="IXT21_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4869576.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
     gene            789..1820
                     /gene="queA"
                     /locus_tag="IXT21_002195"
     CDS             789..1820
                     /gene="queA"
                     /locus_tag="IXT21_002195"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ4869577.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            2439..5078
                     /locus_tag="IXT21_002196"
     CDS             2439..5078
                     /locus_tag="IXT21_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4869578.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(5246..5944)
                     /locus_tag="IXT21_002197"
     CDS             complement(5246..5944)
                     /locus_tag="IXT21_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ4869579.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            6162..7307
                     /gene="tgt"
                     /locus_tag="IXT21_002198"
     CDS             6162..7307
                     /gene="tgt"
                     /locus_tag="IXT21_002198"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ4869580.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            7404..7784
                     /gene="yajC"
                     /locus_tag="IXT21_002199"
     CDS             7404..7784
                     /gene="yajC"
                     /locus_tag="IXT21_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ4869581.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            8090..10687
                     /gene="adhE"
                     /locus_tag="IXT21_002200"
                     /gene_synonym="adhC"
     CDS             8090..10687
                     /gene="adhE"
                     /locus_tag="IXT21_002200"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ4869582.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            10916..12022
                     /locus_tag="IXT21_002201"
     CDS             10916..12022
                     /locus_tag="IXT21_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ4869583.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            12039..13063
                     /locus_tag="IXT21_002202"
                     /pseudo
     CDS             12039..13063
                     /locus_tag="IXT21_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            13069..13548
                     /locus_tag="IXT21_002203"
     CDS             13069..13548
                     /locus_tag="IXT21_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ4869584.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            13564..>14119
                     /locus_tag="IXT21_002204"
     CDS             13564..>14119
                     /locus_tag="IXT21_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ4869585.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         4521 a         2543 c         3096 g         3959 t
ORIGIN      
        1 gcttcatcca tgtcatgggt tacaaatacg atcgtttttc ccaaacgctc ttgtaaatct
       61 ttgacaaggt cttgcaagga atcacgagtg attggatcaa gcgcaccgaa tggctcgtcc
      121 atcaagatga tatcttgatt cgctgcaagg gcacgaacga ctccaatacg ctgctgctga
      181 ccaccagata attcatgtgg gtagcgatct aacatctcac ggggcaattc cacaagatca
      241 atcatttttt cagctgtttt attccgttct tcttgactga ctttcaacaa tttttggaca
      301 agagtgatat tttcacggat cgtcatgtga ggcatcaatc caatactttg gataacatag
      361 ccgatctgac gtcgcaattc cactggatca atttcttgga tggattttcc atcaatcaaa
      421 attttccctt gtgttggatc agtcattcgg ttgatcatcc gcatcgtcgt cgtttttccg
      481 cttccgctcg taccgataaa acaaatgaac tcaccctttt caaatgaaag attgacgtcc
      541 tctacagcga cttttccacc tttataaatt tttgacacat tttgaaattc aatcatggtg
      601 ccacctcttt ttctattttc aatgaagaac tatctattac tagtatgcct aaaacaatga
      661 aaaaatacaa atagggtagt ttttttattt aggaaaacaa aaaatagaag tgaaaaagtg
      721 ctgattttgc caacagaacg aaaaacatga tagaatgatg aagcacatga tagaaagtga
      781 gtgacgatat gttaacaaca gaagattttg attttgactt gccggaagaa ttaatcgcac
      841 aaacaccatt aaaagaccgc gatcattctc gtttacttgt tttggatcgg aaaactggag
      901 agatggaaga taaacatttc catgaaatca ttgaagagtt acagcctggt gatgcgctag
      961 ttatgaataa tacacgagtt ctgcctgctc gtttatatgg agaaaaacca gatactggtg
     1021 cgcatttaga agtccttctt ctaacgaata cacaaggaga tacgtgggag acattgatca
     1081 aacctgcgaa acgtgccaaa gttgggacaa gaatctcttt tggtgatggc agattgcaag
     1141 cagtagtgaa agaagaattg gaacacggcg gacggatcat tgactttgag tatgaaggaa
     1201 tttttctaga gattcttgaa tcattgggag aaatgccatt gcctccatat atcaaagaac
     1261 gtctggaaga cccagatcgc tatcaaactg tctatgcgaa agaaaatggt tctgctgcag
     1321 cgccaacagc tggattgcat tttacgaaag aattacttga tgagatcgaa gcaaagggtg
     1381 ttgaattagt ctatctgaca ctacatgtag ggttaggtac attccgacct gtcagcgtag
     1441 ataaaattga agaacaccat atgcatagtg aattctaccg tttgacggaa gaagcggctg
     1501 agcgattgaa ccaagtacgt aaaaatggcg ggaaaatcgt ggctgtcgga acaacttcaa
     1561 ttcgaacatt agaaacgatt ggaacgaaat ttaatggaga aatcaaagca gacagcggat
     1621 ggactgatat tttcattacg cctggctatg aattcaaagt ggtagaagct ttctcaacta
     1681 atttccattt gccaaaatcg actttagtca tgttagttag tgcctttgca ggaagagaat
     1741 tgactttatc tgcttaccag catgcgattg aagagcgcta tcgtttcttt agttttggag
     1801 atgcgatgtt cgtgaaataa ggttatgaaa agccgctagt tttgaataac aaaaaagctt
     1861 tacaaagatt ttatgacaaa gaaaaagccg agctctcgat aagctcggct ttcttgtacc
     1921 tcggaaggat tcaaataaac agaaatgatt gtaactttcc atcatacatg caaagcacgg
     1981 tgttgaattt cttcatgtta aacaaaaatt ttgttttcgt tacatcttca acatttgaaa
     2041 catctcttgt tcctattcaa ttttcagacg tttacgttat tcaattgtta cgtatcgcat
     2101 tacttcttgt ttcggatact tcgtgatcca aacatccata cgcattcact atttataggt
     2161 ttgtactcta cacgcatgtc tgggtgaaac ctgtacttct aacaactttg tttgcttaca
     2221 ctgttctatc gagaaattct tctgtctatt tgatggtatc ctccttcctt acatctatat
     2281 aatagcatcc tttggtagcg tttgcaaata taagcacttt aaattttacg ctttcttaac
     2341 aaataatcga taagcccttt atttgaagta aataaaaaaa ggatgttata gtaaaataag
     2401 tacctttttt tgtacgaaga ttttaaagga ggaataggat ggaagaatac aagcagacaa
     2461 tagaagaagt aaagaaggta acagaagcag atccagatac tgggttgtct tcacaagaag
     2521 tggatgaacg gcgaaaaaag cagggattga ataaattcga tgaggcgccg aaagaatcga
     2581 tgatcaaaaa attctttcga agcttgtccg attttacaac gatcatcctt cttgtagcag
     2641 cggtgatttc cttttatacc gcatttgcta cagaacatgg ggatttattt gaaggactgc
     2701 tgatcatcgc tatcgttgtg atcaactcgg tattggcgat tgtccaagaa ggaaacgcag
     2761 aaaaagcact ggaatccttg caagatatga ataaacaaac ggcaactgtc atacgagacg
     2821 gcaaagtgac cactgttgag tcagagcaac tggtagtggg tgatattctt gttttagagt
     2881 ccggtgatgc tatttctgcc gatgcccgtt tgatcgaagc atcacaatta cgtgtagaag
     2941 aatcggcgtt gactggagaa agtgaagcag ttgaaaaaga tgcagcattt attgctgaag
     3001 aagatgagtc attaggtgac caacttaata tggtatttaa aggatgtacg gttgctgcag
     3061 gtcgtgggaa agcgatcgtc actgccattg gcatggctac agaaatgggt aagatcgcgg
     3121 atcttttgaa cgaaaatact atgcaaaaaa cacctttaca ggtccgattg aatcagctag
     3181 gcaaacgaat cagtatgatc gctttagcgg ctgcggcttt agtatttgtg attggtgaac
     3241 tgcaaggaga accactcctt gagatgttca tgacttcgat ttccttggct gttgcagctg
     3301 taccggaaac cttgactgtt attgttacgt tgacacttgc atacggtgtg cagaagatgg
     3361 cgaggaaaca tgcgatcatc cgacagttgc cagcagttga aacgctagga accgctaatg
     3421 ttatctgctc agacaaaaca ggaacgctga cacaaaatga aatgcgcgtc cgccgggtat
     3481 ggagcaaaga agatgaagta acgaatatcg aagattcaat gacgaacagc gcgatggaaa
     3541 tattgaaaat ggctgcacta tgtacagatg tgacggtcga gcaagaagat gacgaccttg
     3601 ttatcaaagg taatccaaca gaagtagcca tcgtaagggc tgttgaagag aattatcata
     3661 caaaggcaga attagaagaa aaatatccac gagtaaacga gcttcctttt gattctgaac
     3721 gaaaaatgat gacaacagtt caccaaatgg gcgaaaaata catctcaatc accaaaggag
     3781 cattcgatgt tcttgctccg cgctttagtt caggcgatgt agaacaagcg gcgatcgtta
     3841 atgatcgctt tggtaaacgg gcactgagag tgattgctgt tggttatgct acttatgacg
     3901 aagaaccgca agatatttct tctgaagcac tggaaaaaga cttacgtttg attggtctga
     3961 tcggaatgat cgatccacca cgtccagaaa gtaaaggagc aatcaagcga gcgaaaaagg
     4021 ctggcatcaa aacagtcatg atcactggag atcatgtggt gactgccagt gccatagcga
     4081 aagaattagg tatcttgaat gatccgtcag aagcactttc aggatcagaa ctgcatcaat
     4141 tatcagacga agaattagat gcgcgtgtca aagctctctc tgtctatgct cgggtaacgc
     4201 cagaagacaa gatccggatc gtcaaatctt ggcaacgtac gggtgcagtc gtagcgatga
     4261 ctggggatgg tgtgaatgat gcaccagcgt tgaaagcaag taacgttggg tgtgccatgg
     4321 ggatcacagg aacagatgtg gcgcaaagtg ccgcagatat gattttgacg gatgacaact
     4381 ttgcgacgat cgtagacgct gtcagtcaag gacgttcggt ttatcagaat attcgtaaag
     4441 ctatcaactt tttgctaagc tgtaatattt cggagatctt tattgttttg attgcgatgc
     4501 tgttaggatg gggagcacca tttacagctg tgcaactact gtttgtcaat gtagttgctg
     4561 atggacttcc aggatttgct ttaggaagag agccagcgga acacggcatc atggatcagc
     4621 cacccattcc gaaaaatgaa gggatctttg ctcgaggttt attgcaaaaa attgccataa
     4681 atgcaggggt cttcaccatc gtaaccttgt ttggttatta tttaggtagt tatgtcgata
     4741 ctatcagccc atgggtagac gcaagtcagc atgtcggtca aactgttgca ttcttgatct
     4801 tagcttattc atcgattctg catgtgttca atgtacgcag cagccaatcg atcttcaaag
     4861 tcaatttagc gaccaataaa gctttggtag aaatggcgtt actggcatta gctattacaa
     4921 cggctgttgc tttgctgcca tttacgcaag aactattcgg cctagtacac atcagtttga
     4981 atcactggtt cttggtaggg attttatcta tcgtaccaat cgcggtgaat gaactgatca
     5041 agttccatcg attaccggaa gcagaagaag aggaatgatt aactgtttct aagttgattt
     5101 tctttttact ggaactatac aaaaaggagg tgtgacatga atgttgtcac acctcctttt
     5161 tgctaatggg ctgaatgttt cttttactgc ctctcgataa acggtgttct cctgccaccc
     5221 cctcggtcac aagtcacact ctcttttaaa tatcttttgg caattgttca taaaatgctg
     5281 cttttgttgc agaaatgtat gggctgagcc atagataacc aataccagca gttgctaatg
     5341 ctaagatatg ccagccgata aatgaaagat ctaaccagaa aagttgtcct ttataaccat
     5401 ccatcaattt tcgacttgca gtgatcgtat ccagtacttt aggtgtttgt cctgtttctt
     5461 ggacgatatc gtagtaaata aaatagcttt gagaataaga atagctttta atgattccag
     5521 gaataactaa tagcaaaccc cataatgttg taaagatcga gatcagcaca gctaagctga
     5581 tcactccgcc gataaacaaa cctttgaatc cacgaaatgc atctttgaat ggttcgatct
     5641 gatctttttg tccacgtaaa atatcaagat aagtccaaga aataccgctc atgaacaaag
     5701 cgctaatgat gctggaaata attccgccac cgcctcctga actaccgcct cctgaagttg
     5761 tcgcttctga gacgctcgct gatccatcat atatggtggt ggcaaacaag ataataggaa
     5821 tcataataag aagaatagta gcaataccaa tcaatgtagg gattagattt agtaaaacag
     5881 cttgtcccca acgtcctttt agactctctt ttgcttggcg ttttaattct gcagaagatt
     5941 gcatgatttt tccctccttt tctttaataa caccaatata ccatgtatca aaaaatattt
     6001 ctgtcataat gtttttcttt tttttgttat tcataaaaaa aggataaaaa aaagaatatt
     6061 aaataaagta gaaggaagcc gagggaactt gcaaaaagca cttgaaacga ttatgatggt
     6121 gggagttgtc aagatatttt atatagaaag tagggaaatg aatgacgcaa cctgccattc
     6181 gctatcgttt aatcaagaaa gaaaaacata ctggcgcgcg tttaggggaa ttgatcacgc
     6241 cacacggaac tttccccaca ccaatgttca tgccagtagg aacattagct acagtaaaaa
     6301 caatgtcccc agaagagttg aaagaaatgg gtgctggcgt tattttaagt aatacatatc
     6361 atttatggct acgccctggt gaagacttag tagaagaagc tggcggactg cataaattta
     6421 tgaactggga tcaaccgatt ttaacagatt caggcggctt tcaagttttt tcattaagtg
     6481 atatgcggaa tatcgaagaa gaaggcgtgc atttcaaaaa tcacttgaac ggttcgcgta
     6541 tgtttttatc accagaaaaa gcaatcaaca tccaaaataa attgggctct gatatcatga
     6601 tgagttttga cgaatgtcca ccattcgatg aaagttacga ctacgtgaaa aaatcagtag
     6661 aacgtacgtc tcgatgggca gaacgaggat tgaaagctca tgctaatcca gaccgtcaag
     6721 gattatttgg aatcatccaa ggagcagggt ttgaagatct tcgtcgtcaa agcgcgaaag
     6781 atctagtcgg gatggatttc cctggttact caatcggcgg gttatctgtc ggggaaccta
     6841 aatcagaaat gaatcgtgta ttagaattca ctacaccgtt gatccccgaa aacaaaccac
     6901 gttacttgat gggtgtcggt gctgctgatt cattgatcga tggtgttatc cgtggagtag
     6961 acatgtttga ctgtgtcttg cctactcgaa tcgctcgaaa tggcacatgt atgacttctc
     7021 aaggccgtct agtagtgaaa aatgcgaaat atgcccatga tttccgtcca attgatgaga
     7081 aatgtgattg ttatacatgt aaaaactata cacgtgccta tatccgccat ttgatcaaat
     7141 gtgatgaaac atttgggatt cgtttgactt cttaccacaa tttatacttc ttgttgaatc
     7201 taatgaaaca agtacgtcaa gcaatcatgg atgataattt gcttgaattt agagaagcct
     7261 tttttgaaga atacgggttc aataaagaaa acgcaaaaag cttctaaagg aaaatctttt
     7321 aaaaattaag taaaaaacta gtgttatcag catttttctg ctaaagtgtt ttttgtagat
     7381 acagaattag tgaggtgaaa aaaatgggaa cattaccaat gcttgtgatg tttatcgcgc
     7441 tattagcaat gtggtttttt atgtcacgtt cgcaaaagaa acaacagcaa gaacgtcaaa
     7501 acttgttaga tagcatgaaa ccaggtgatt cagtcgtaac gatcggcgga ttacatggag
     7561 ttgtttcaga aatcgacaat gacaaacgga cagttgtttt agactgcgaa gggatcttcc
     7621 ttgagtacga tcgtgcagca atcaaaacgg tcaaaccagg gactgtcgta acaaatgatg
     7681 caacgactac tgttgaaaca acagaagaac cagctgagac agttgaagta aaaactgaaa
     7741 caacagaaac gctggaagag acagaagaga aggaagaaaa ataagtatca aagcttattt
     7801 aaagacgagg tagggcagaa agatgtccta cctcattttt tattcaaaaa agaaagagat
     7861 aagagcatgg gatatattgc tcaatattga ataatcgggt gtgtcatata aataataata
     7921 aataacttta tatatttttc tatacaaaca cctgtaaata aagcaatttt atatttaaaa
     7981 ataactgtga aaaaaaatca caaaaaagtg tttacataga aaaataaata ttgtatgata
     8041 tacatgtgaa ttatttaaca aataaccatg acccattagg aggataatga tggctaaagt
     8101 aatagaaaaa gaaaaaacga agacaattga cgtacaagca atgattgatg atttggcaga
     8161 aaaagcaaat gcagcattga aagaaatgga aagctttgat caagaaaaag tagaccatat
     8221 cgtgcatgaa atggcaatgg cagctttgga tcaacatatg ccattagcga agatggctgt
     8281 agaggaaaca ggccgcggtg tttacgaaga taaagcaatc aaaaatatgt atgcgtccga
     8341 atatatttgg cataatatca aacatgataa aacagttggt gtgatcaatg aagatgtcca
     8401 aaaagggttg attgaaattg ctgaaccagt cggtgtggtt tgcggagtaa ctccaacaac
     8461 gaatccgact tccacaacga ttttcaaatc aatgatcgcg ttgaaaacaa gaaatccaat
     8521 cgtttttgct ttccatccaa gtgcccaaaa atcttctgca gaagcagcac gagtggtacg
     8581 tgatgctgct atcgctgccg gagctcctga aaattgtatc caatggatcg aacatccttc
     8641 gatcgaagcg acttctatgc taatgaacca tccaggtatc gcgatcgtct tagcgacagg
     8701 tggagctgga atggtcaagt ctgcttactc tacaggaaaa ccagctttag gtgtaggtcc
     8761 aggtaacgta ccagcttata ttgaaaaaac agcaaaaatt aaacgtgcag tgaacgattt
     8821 gatcgtttcg aaaacatttg ataatggaat gatttgtgct tctgaacaag ctgtgatcgt
     8881 ggataaagaa atctatgcag cagtaaaagc agaattccaa gcacaccaag tttatatcgt
     8941 aaaaccagat gaattgcaaa aattagaaga tgcagtgatg aacgaaggaa aatatgcggt
     9001 caatccatca atcgttggac attctgcgat ggaaatcgca aaattggcag gaatcagtgt
     9061 accaaaagga acgaaaatgc tgattgctga attggaaggc gtaggcccag attatccgct
     9121 atcaagagaa aaactttctc cagttcttgc catgatcaaa gctaacaaca cggaccatgc
     9181 gtttgatttg tgtgaaggta tgttgaatct tggaggatta ggacatacag cagttatcca
     9241 ttcagaaaac gaagagttgc atgtcaaatt cggtcttcga atgaaagctt gtcgtatttt
     9301 agtgaataca ccatctgcag aaggcggcat cggtgatatc tataacgaaa tgattccgtc
     9361 attgactttg ggatgcggtt cttatggtaa gaactctgtt tctcgtaacg tctctgctgt
     9421 aaacttaatc aatgtcaaaa ctgtagcgaa acggagaaat aacatgcaat ggttcaaatt
     9481 accgccaaaa atcttctttg aaaagaactc tctattgtac ttagagaaaa tggaaaacgt
     9541 tgaacgtgtc atgatcgttt gtgaccctgg tatggtccaa ttcggttact gcgatacagt
     9601 tcgtgaagta ttggcacgac gtaaaaatga tgtgaagatc gaagtcttct cagatgttga
     9661 accgaaccca tcgacaaata ccgtatacgc aggaacgaaa ctgatggcag acttcaagcc
     9721 agatacagta atcgctcttg gaggcggttc tgcaatggac gcagctaagg gaatgtggat
     9781 gttctatgaa cacccagaca catctttctt tggtgcaaaa caaaaattct tggatatccg
     9841 taaacggaca tataagatcg acaaaccaga aaaaacacaa ttcgtatgta tcccgacaac
     9901 atcagggact ggttctgaag taacgccatt tgcggtcatc actgacagtg aaacacatgt
     9961 gaaatatccg ttagcagatt atgcattgac gccagatgtc gcaatcgtcg atcctcaatt
    10021 cgtcatgtca gttccagcat ctgtcacagc agataccggg atggatgtgt taacacatgc
    10081 aatcgaatcc tatgtttcag tcatggcttc agattataca cgtggattga gcctacaagc
    10141 aatcaagtta gtctttgact atttagaaaa atcagtaaaa acacctgata tggaatcacg
    10201 tgaaaaaatg cacaacgctt ctacaatggc tggtatggcg tttgctaacg cattcctagg
    10261 tatttgtcac tcagtcgcac ataaaatcgg cggggaatac gggatccctc atggacggac
    10321 aaatgcgatt cttttaccgc atattatccg ctataatgca aaagatccac aaaaacatgc
    10381 gatgttccct aaatatgact atttccgtgc agatacagat tacgcagata tcgcaaaatt
    10441 cttaggtctg aaaggcgaaa caacagagga attagtcgaa gctttagcaa cggctgttta
    10501 tgaattagga aaatctgtcg gcatcaacat gagtctaaaa gctcaaggag tcacacaaga
    10561 aacactagat acaaccgtag accgtatggc tgaattagct tacgaagacc aatgtacgac
    10621 agctaatccg aaagaaccat tgatctctga attgaaacag atcatcattg atgcttacaa
    10681 cggataagca aaagaaagcg tagaaaaaag ataagaaaat agatgaaaaa gaacttggaa
    10741 aacgttgttt ctccaagttc tttttttaat ttccaagtaa ctgaaagtta cttttgttat
    10801 ttaacattta ggtcaatttt agacagtttt tgttcaaaaa gcgatattta tagtggacgg
    10861 tattactgct atattaagga tataaaatga aagcgtttaa aaaggtggcg taaagatgat
    10921 tattaaagaa gagatttttg tacctaaaga attagtgaaa gaaaaaattg acctgctcgt
    10981 tgaaaacctg acaacgatca aagacgataa tggtgaattt ttgttggact ttgatggact
    11041 gaaagtagat gacaaaagct ggacagtgtg gaattggcca caaggtgtcg gtctatatgg
    11101 tatttataaa aattaccaga tgacaaaaaa tcctcgcgct tatcaagtgg tcaatgaatg
    11161 gtttgaggat cgtatggaag aaggcgctcc gcccaaaaac gtcaatacaa tggcaccgct
    11221 attgactatg gcttacttgt atgaagacac gaaagattca cgctatttgc cttatttaga
    11281 acaatgggca gaatgggtca tgaatgacat gcctcggaca aatgaagacg ggttacagca
    11341 tgccacatat ggaccggaaa ataagaatca gttgtgggac gatacattga tgatgacagt
    11401 gcttcctttg gcaaaaatcg ggaaactgtt gaatcgccca gaatatttag aagaagctcg
    11461 ttaccagttt atgatccata tcaaatactt gatggataaa cgaacaggat tgtggtatca
    11521 cggctggacg tttgaaggaa atcataacta tgccgaagca ttttgggctc gtggaaactg
    11581 ctggttgacg attgctattc cagagatcat tgaaatctta gaattatcga aagaagatgc
    11641 attgcgcaaa ttattgatcg aaacacttga agcacaagtg cgtgcattaa aaacttatca
    11701 gtctgaaagc ggtttatggc atacgttgtt ggatgatcct tcttcttacg tagaatcttc
    11761 tgcaactgca ggatttgctt acggtatttt gaaagcggtc cataaacgtt atttaccaca
    11821 agaatataaa gaagtcgctt atcgtgctat ccaaggattg cttgaacaaa tcgatgaaaa
    11881 aggagaagtt caaaacgttt caatcgggac aggaatgggg gacagtttag atttttatcg
    11941 gaatatcgga atcacagcaa tgccttatgg acagtcgtta acggtgcttt gtttgacaga
    12001 attgctcgtc tcttattgtt aaaagaaagg aaacccatat gaaaaaaaca tcactgctgt
    12061 ttgcagcaag ttcgcttagc ctaggtctgc taggtctaag cggctgcgga aataattcta
    12121 gtgtggcaac tgtcgcagat gcagacgtaa atgagatagt taccttgcgc tttgcttatg
    12181 caagcaacag ccagtaattg atgcaatgaa tgaatttggg cgtttagtaa aagaaaaaac
    12241 agatggttcc gtacagatcc agtatttccc agatggacaa ctgggtggag aacgtgaact
    12301 gatcgaattg acacagtcag gagcagtaga tttcacaaaa gtaagtgcct cagcgttgga
    12361 aagtttctct aaagattatt cgattttttc cgttccttat ctattcaatg atgaagaaca
    12421 tttctttaag gtgatggata atgaagaaat aatggagcca atctaccaat cgaccaaaaa
    12481 tttaggattt accggattga cctattatga ttctggtcag cgcagctttt atatggtaga
    12541 cggtccgatc catacgccgg aagatttgaa gggaaagaag atccgtgtga tgcaaagcga
    12601 aaccgctatc aaaatggtag agcttttagg cggttcgccc gtacctatgg gaagtgatga
    12661 agtgtacacg tctcttcaat ccaatttgat caacggatca gaaaacaatg aatttgtctt
    12721 gtatacagct ggacatggcg gggtagctaa atactattcc tatgatgagc atacacgtgt
    12781 gccagatatc atcatcatga atgatgccat caaagaacag ctaactgatg aacaggaaaa
    12841 agccattgaa gaagcagcga aagaatcaac ggtttttgaa attgaagctt ttgcaaaagc
    12901 aattgaagaa gaaaaagctt tagcacaaga agaacatgga gtccaattca atgatgtaga
    12961 taatacaccg tttcgagagg cagttgcccc acttcatgat atattcaaaa acgatcctac
    13021 ctacaaagac ctttatcaaa agatccaaga gcaaggagcg tagctgtaat gaaaatgatt
    13081 ttaaacaagg tattagagat cctgggagct gttttgcttg ttgggatggt cgctgttgtt
    13141 ttatggcaag tattttccag aacggtttta ggaaatccaa atacggtcac ggaagagctt
    13201 gttcgattcg gtcttgtctg gtttgccatg ttgtcttccg cgtatgttgt gggacaaaaa
    13261 gcacgtttag ccgtaacggt tttaagtgaa aaattaactg gcaaaaaaag taaaatttta
    13321 gaagttgttg ttcaaagttt atttctggta tttgctgcga tgatcatggt gtatggcgga
    13381 tggaatgctg tcacattgac aatggggcag atctcgcctt ctttaagtat tcctatggga
    13441 tatgtttatc tgtcagtacc tgtatcgggg gtactcatca ttatctatag cctgatcaat
    13501 ctaatcgata cattaagcaa caagccagca attcaagaaa aaatataaaa atggaggaat
    13561 aaaatggcaa tagaagccgg attagtgtta ttttttgtat ttgttgtgtt gcttgtatta
    13621 gggatgccga ttgctataag tattgcattc tcatcgatga tgactttatt gttagttata
    13681 ccgtttgatg tttctgcttt tagttcagct caaaaaatgg tgggaagtat caatagtttt
    13741 tcgctgatcg ctattccgtt tttcgttctc tcgggcatta tcatgaataa tggagggata
    13801 gctaagaaac tagtggattt tgcgatgttg ttcgtcgggc gcgtgccggg ggctttggct
    13861 catacgaatg tgttagggaa tgctttattt ggttccatct ctagctcggc cattgccgct
    13921 tctaccgcga ttggtggagt gttgatccct cagcaagtgg aagaaggata tgaccggaag
    13981 tttgcgacag cggtcaatat cgcgtcagct ccgacaggta tggtcattcc tccaagtact
    14041 gcttttatca tgtattcact tgttgctggt ggtgcttcga tctctgcttt gtttatggga
    14101 ggttatctcg taggcgctt
//
LOCUS       DACGLH010000070        14104 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.075, whole genome shotgun sequence.
ACCESSION   DACGLH010000070 DACGLH010000000
VERSION     DACGLH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.075"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            65..766
                     /locus_tag="IXT21_002205"
     CDS             65..766
                     /locus_tag="IXT21_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ4869586.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            763..1884
                     /locus_tag="IXT21_002206"
     CDS             763..1884
                     /locus_tag="IXT21_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ4869587.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1899..3131
                     /gene="pepT"
                     /locus_tag="IXT21_002207"
     CDS             1899..3131
                     /gene="pepT"
                     /locus_tag="IXT21_002207"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ4869588.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3263..4171
                     /locus_tag="IXT21_002208"
     CDS             3263..4171
                     /locus_tag="IXT21_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ4869589.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4206..4478
                     /locus_tag="IXT21_002209"
     CDS             4206..4478
                     /locus_tag="IXT21_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ4869590.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4489..5535
                     /locus_tag="IXT21_002210"
     CDS             4489..5535
                     /locus_tag="IXT21_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ4869591.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5786..8395
                     /gene="clpB"
                     /locus_tag="IXT21_002211"
     CDS             5786..8395
                     /gene="clpB"
                     /locus_tag="IXT21_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ4869592.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8546..9217)
                     /locus_tag="IXT21_002212"
     CDS             complement(8546..9217)
                     /locus_tag="IXT21_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869593.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9210..9704)
                     /locus_tag="IXT21_002213"
     CDS             complement(9210..9704)
                     /locus_tag="IXT21_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ4869594.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9724..10944)
                     /locus_tag="IXT21_002214"
     CDS             complement(9724..10944)
                     /locus_tag="IXT21_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869595.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11091..12926
                     /gene="lepA"
                     /locus_tag="IXT21_002215"
     CDS             11091..12926
                     /gene="lepA"
                     /locus_tag="IXT21_002215"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ4869596.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13020..14102)
                     /locus_tag="IXT21_002216"
     CDS             complement(13020..14102)
                     /locus_tag="IXT21_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4869597.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4554 a         2592 c         2920 g         4038 t
ORIGIN      
        1 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
       61 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
      121 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
      181 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
      241 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
      301 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
      361 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
      421 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
      481 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
      541 tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga tgttcgggcc
      601 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
      661 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
      721 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
      781 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
      841 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
      901 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
      961 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     1021 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     1081 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     1141 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     1201 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     1261 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     1321 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     1381 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     1441 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     1501 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     1561 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     1621 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     1681 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     1741 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     1801 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     1861 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     1921 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     1981 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     2041 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     2101 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     2161 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     2221 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     2281 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     2341 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     2401 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     2461 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     2521 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     2581 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     2641 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     2701 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     2761 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     2821 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     2881 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     2941 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     3001 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     3061 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     3121 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
     3181 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
     3241 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
     3301 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
     3361 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
     3421 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
     3481 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
     3541 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
     3601 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
     3661 cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga tcagtcaaga
     3721 agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt tattgctgaa
     3781 gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag gaaaatccca
     3841 actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg acgactcagc
     3901 gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata ttttccgatt
     3961 tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg gcattactta
     4021 tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga
     4081 tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag gattaacagt
     4141 ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa gttggaagaa
     4201 gagtaatgga tgtattgtta cacaatcttt tgtggtttta ccttttcatt gtgaattttt
     4261 acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc tggcgtgttc
     4321 ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga ttgatctctc
     4381 gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc gctgcaggtg
     4441 tacttgttga tttgattctc atttattttt tccattaagg agcaggagat gtcgtctaaa
     4501 ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt tgccattatt
     4561 tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc taccagtgtt
     4621 gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga agggcgctca
     4681 atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg gttcttgacc
     4741 tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct gatctcggag
     4801 atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt gacagcactc
     4861 catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt ggtcatccaa
     4921 ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc tggtatcctt
     4981 ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa tgttcaaata
     5041 ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa atgggtggat
     5101 acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat ccaagacagg
     5161 actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg gtatgtactt
     5221 ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac catttcgttt
     5281 atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat tggttccttt
     5341 gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc agtttcttcg
     5401 cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt tgtgtatgtg
     5461 attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa agagaataaa
     5521 gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa agaaaaacgt
     5581 tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac aatcaatttt
     5641 gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaaccatgtg tatattttga
     5701 ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata agtgatctta
     5761 cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga cgcttcaaga
     5821 agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata tcgacattgc
     5881 ccatgtatgg aaaatctttt tacaacctaa tcattttggt cgaaatttct atacggatgc
     5941 cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg agtatccagt
     6001 tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct ttcgattatt
     6061 gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt
     6121 tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga ataaaaacgg
     6181 attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag aaagagtgac
     6241 ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg atttagtaca
     6301 gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa tccgtgatgt
     6361 catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag aacccggtgt
     6421 agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga
     6481 aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg ctggagcaaa
     6541 attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca aaaaaagtga
     6601 tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga
     6661 aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg aattacattt
     6721 gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca aagcgttgga
     6781 acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga tcagtattct
     6841 gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg acaacgcatt
     6901 agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac cggacaaagc
     6961 aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact ctatgccaac
     7021 ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag ctgcactgaa
     7081 aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga
     7141 acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa
     7201 ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag aagatgcaga
     7261 aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac ctcaattaga
     7321 aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga tggttcaaga
     7381 gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta tccccgtaac
     7441 gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac ttcataaacg
     7501 agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc gttcacgggc
     7561 tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac caactggtgt
     7621 cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag aagatcatat
     7681 ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc tagtcggagc
     7741 tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg tcagacgaaa
     7801 tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg tcttcaatat
     7861 cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag tagtagattt
     7921 caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc tggaaggcgt
     7981 gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg
     8041 aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta caccattgag
     8101 cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac aacgtctgga
     8161 acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg ctgaaaatgc
     8221 ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg aagtagaaac
     8281 accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag taacgattac
     8341 tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg tctaatcttc
     8401 cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc tccttttttc
     8461 aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt cttgaagctg
     8521 cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat ttataacttt
     8581 caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt aaggcagcac
     8641 ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca
     8701 gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg tcgcataaga
     8761 attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg aataagagga
     8821 acaaccaagg actggttttt gatatctcag catctttcat caagtaacca atcaatccat
     8881 agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc caaaaagaga
     8941 tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag aaaaaccagt
     9001 aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg acttgtggca
     9061 tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct tgagaagtcg
     9121 aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca ccaaaagcaa
     9181 gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc atctaaaaat
     9241 ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt caatttgatc
     9301 aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc ttgtagtgca
     9361 gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa gccgatcact
     9421 gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc gatcgtcagc
     9481 ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc agcaagagca
     9541 atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc ttgaggaaga
     9601 tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg gttatataac
     9661 aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac atcatccttt
     9721 ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct ctagtgactt
     9781 cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag cacggcgata
     9841 attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc ctataggttc
     9901 atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac cattattcca
     9961 aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa caaacggaaa
    10021 ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt cgatctgctc
    10081 tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa tatctccatg
    10141 tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat ttcgagcttc
    10201 ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc agtagaacag
    10261 aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct catcttcatt
    10321 ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac catggttttg
    10381 ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta atcgctcaac
    10441 cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa aggaaagttt
    10501 atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag gatacaaata
    10561 agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta gatcttgcat
    10621 caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac ccattttata
    10681 acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga tcagtttatt
    10741 ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg aacggataaa
    10801 agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt ggaacacata
    10861 ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta attcaagatt
    10921 ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga taacagtata
    10981 gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt aaattactgc
    11041 ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct atggatataa
    11101 agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct catatcgacc
    11161 atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg acgtctcgcg
    11221 aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga atcacgatta
    11281 aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat atctttcact
    11341 tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc cttgctgcct
    11401 gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa acattggcca
    11461 atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat aaaatcgacc
    11521 tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg
    11581 ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat attctcgagc
    11641 agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta aaagctttga
    11701 tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg atcacggacg
    11761 gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta
    11821 cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg
    11881 tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg gatacagtaa
    11941 cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg aatccgatgg
    12001 tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg gaagctttag
    12061 aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct caagcattag
    12121 gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc caagaacgct
    12181 tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt taccatgtta
    12241 ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct gaacctgtaa
    12301 cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca aatgattttg
    12361 tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg atggattatt
    12421 tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt gtctttgact
    12481 ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat gaaatgtctg
    12541 gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa aaagtcgatg
    12601 cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca atcgttgaaa
    12661 aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca gctattggac
    12721 aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt
    12781 atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc
    12841 ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa
    12901 tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt aaaaaataaa
    12961 ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg tggcaaaaat
    13021 caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat cttatctgtt
    13081 acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg ttcagagatt
    13141 tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct gagagaatgg
    13201 aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt cactacactt
    13261 ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat gtagagcttt
    13321 ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat tgctagcaag
    13381 gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc atatacccat
    13441 gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga aaaatcgaca
    13501 tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa tccaatattc
    13561 aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc aaagtcctca
    13621 agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt tggtattttt
    13681 acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat attcagcgca
    13741 ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt tttaaattca
    13801 gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt ctttcctaaa
    13861 taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt gtattttaga
    13921 tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg agtatccgta
    13981 cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa agcagcttcg
    14041 gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc atcatcattg
    14101 atat
//
LOCUS       DACGLH010000071        13917 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.076, whole genome shotgun sequence.
ACCESSION   DACGLH010000071 DACGLH010000000
VERSION     DACGLH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.076"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            131..505
                     /locus_tag="IXT21_002217"
     CDS             131..505
                     /locus_tag="IXT21_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ4869598.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IXT21_002218"
     CDS             complement(619..1248)
                     /locus_tag="IXT21_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4869599.1"
                     /translation="MKIVFFHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IXT21_002219"
     tRNA            complement(1382..1454)
                     /locus_tag="IXT21_002219"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IXT21_002220"
     tRNA            1668..1741
                     /locus_tag="IXT21_002220"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IXT21_002221"
     CDS             1696..1845
                     /locus_tag="IXT21_002221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869600.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IXT21_002222"
     CDS             2338..3666
                     /locus_tag="IXT21_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ4869601.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IXT21_002223"
     CDS             3868..4701
                     /locus_tag="IXT21_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ4869602.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IXT21_002224"
     CDS             4717..5454
                     /locus_tag="IXT21_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ4869603.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IXT21_002225"
     CDS             complement(5609..6577)
                     /locus_tag="IXT21_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ4869604.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IXT21_002226"
     CDS             6809..7642
                     /locus_tag="IXT21_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ4869605.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IXT21_002227"
     CDS             7676..8515
                     /locus_tag="IXT21_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4869606.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IXT21_002228"
     CDS             8541..9101
                     /locus_tag="IXT21_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ4869607.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IXT21_002229"
     CDS             9300..11021
                     /locus_tag="IXT21_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ4869608.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IXT21_002230"
     CDS             11186..12328
                     /locus_tag="IXT21_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ4869609.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IXT21_002231"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IXT21_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ4869610.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4753 a         2297 c         2832 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg aaaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attatttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACGLH010000072        13837 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.077, whole genome shotgun sequence.
ACCESSION   DACGLH010000072 DACGLH010000000
VERSION     DACGLH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.077"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(139..1341)
                     /locus_tag="IXT21_002232"
     CDS             complement(139..1341)
                     /locus_tag="IXT21_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4869611.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1344..1538)
                     /locus_tag="IXT21_002233"
     CDS             complement(1344..1538)
                     /locus_tag="IXT21_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ4869612.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1585..2433)
                     /locus_tag="IXT21_002234"
     CDS             complement(1585..2433)
                     /locus_tag="IXT21_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAQ4869613.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2638..2988)
                     /locus_tag="IXT21_002235"
     CDS             complement(2638..2988)
                     /locus_tag="IXT21_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869614.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3583..4029
                     /locus_tag="IXT21_002236"
     CDS             3583..4029
                     /locus_tag="IXT21_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ4869615.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4653..5645
                     /locus_tag="IXT21_002237"
     CDS             4653..5645
                     /locus_tag="IXT21_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4869616.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5661..6545
                     /locus_tag="IXT21_002238"
     CDS             5661..6545
                     /locus_tag="IXT21_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4869617.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6594..8201
                     /locus_tag="IXT21_002239"
     CDS             6594..8201
                     /locus_tag="IXT21_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4869618.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8202..9686
                     /locus_tag="IXT21_002240"
     CDS             8202..9686
                     /locus_tag="IXT21_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ4869619.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9863..10855
                     /locus_tag="IXT21_002241"
     CDS             9863..10855
                     /locus_tag="IXT21_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4869620.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10907..11776
                     /locus_tag="IXT21_002242"
     CDS             10907..11776
                     /locus_tag="IXT21_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4869621.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12196..13761)
                     /gene="guaA"
                     /locus_tag="IXT21_002243"
     CDS             complement(12196..13761)
                     /gene="guaA"
                     /locus_tag="IXT21_002243"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ4869622.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4240 a         2343 c         2855 g         4399 t
ORIGIN      
        1 aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat tcccactcaa
       61 tatgagtagc ctcgtaaaga agctattata tcagtgcttt ttcagcctat tcctcatttt
      121 ggtagggaaa tggtagggtt agatttttac tgcttgattt ccattaaagt ttgcaacttt
      181 agattttgtt gcacaggtaa atttaatgtt tcctgtcatt tgttctgcaa ttccttcatc
      241 attacggctc catagatgag cgtagtgctt taaagtgatt tcagggctag aatggcctaa
      301 ccgttgcgac acgaccagaa tgtctgcgtt aaactcattg attaggtagc taacatgaga
      361 atgacgtaat ccttttcctt gtattagggg taccttagct agtttggcat aacgctgaat
      421 aattcgttga acagttgaac gatagagtgg gaggtcagtg tagctaagaa taaattgttc
      481 tacaccatga tttttttgaa taactttcca cttttttagc acttcaattg tatcatcgtc
      541 cagagaaatg atccgcaagc cgctttcagt ttttgtgtaa ggtttccgaa taaaatcttt
      601 ttgatttgtc atttccaagg tgtgatgaac cctcaacttt ttacgtttga agtccacgtc
      661 attccaatta agtgctaatc cttcgcttac acgaacacca gtcatgtaat atagccagat
      721 cattaaaaaa cacatatgtt cgtagaaatc atctttataa atttttgcca aaacttcttc
      781 aaattcttct tttgtccagt aaggaacgat tgattttcct tttggaattg cttttgttct
      841 tttggaaata ttctcattca gaaattgcag ggtgaccgca tagtctaatg atttacgaaa
      901 cataccgtac ataagtgaac tgtatgcttg tgagtatccg ctatcgttca ataaccaaat
      961 tcgataattt tcacaatctc gcacattgat ttcacgaagc ttcttatcac cgaaaaattc
     1021 agtaatctga atcaatccac actcacgaga actccaagta cttttttgta cgctagcttt
     1081 gtagtgtgga acgtaagact gctccataaa ttctttgtaa gttaaatcat aattcgcaaa
     1141 tccatttaca accatgtatt cattttttat acgcatgact tctttgtgtg cttcacgagc
     1201 tgatgagaat cgttttccta gagaatcttt tgttcctttc ttctggatcc tctttccagt
     1261 cacttgatct gttcctaact caactagata agaaaagcga ccatttttat cttgatacac
     1321 acaagggtat tttgttttca tagttattcc tcctgtgaaa gaggaacacc tataatttct
     1381 tcaattactt gtcggggtac taccccaact cttttattat tataatatgg atatccctga
     1441 tttaccattc ttgtttttgc ttgtcggatt attgtggtag cttgatgtgt tttaaatcct
     1501 agagcctcta aatcttttcg acaaattgta tttttcattg tataaactca ctttcaaaga
     1561 aaacactatc attgggtaat agtgttattt ctttattttt gtataatgaa cggtactttc
     1621 ttgaaaatcc atcacttgtt caggacttcc caactcattt aaagctaaag cattgtaaaa
     1681 aaatggacgt ctaactatat attcgcttgt atatagtata tcttgattcg tggaatcata
     1741 gggtttcttt accatctttt tggtgacagt tggtagtttg agattccggg acttgaaaaa
     1801 agcttttttc ttatgctctt tcaattccag ctctttatcg aaatatttgc tgatatagcg
     1861 cccgcgattt tcgatactgt ctacatcgat cttgttgatc ttaacgaaac cctgacccca
     1921 aatcgtagtt aaccggttaa atgagataaa ggggaaatca aacaagataa tatggtagtg
     1981 gattgcaccg cgttgctgct tttcccacgt ggcgagatac tttaaatttg cttttttagt
     2041 tttatagagg tcataattca gtcgtttgat aaatttttta aattcattat ttgcatactc
     2101 aatgtcttga atgttttctt tgaaagtgag agttaaaaat ttcgtttgat tgtcaaaatt
     2161 cgtgtctaca aggcgggcaa tctcaaatcg catactttga taatgtttct gtttgcgttt
     2221 taaactgtca tattgtcctt gagcggataa ttcatcatag ttttttcttt tttcaagagt
     2281 aatatcattt tcataaatcc aagctaattt ttgttttcgt tccagattta cttcgttttc
     2341 gatcgtttta cgtttgggag taatgattgc ttcataatca taaatttcaa tatacgtggg
     2401 agtttcaatg attttctgat tataagcttg caaaaatcat cactccttgt tgcgtattcg
     2461 attgtcaaag aaccagtcat tttctcctga tgttgatcta taaatcaagt taagggggaa
     2521 ggcctgcgta gcagcccttc tagcctatgg ggtgttgggc ggtcttaacg gacagaaacg
     2581 gctctgtggg cgctgccccg ccgtttcgtc ctaaccaccc aacactccat agtatagtta
     2641 gttttgtgta gtggtacctt gttttctggg aatcaaagtt gccgtatcag cggttgccga
     2701 taacccataa ctgacaaatt gtccattcga gcgtccccaa ggtacaatca ccaaattggt
     2761 aaatgtaatt atggctaaac cttgttgggt aagttcttcc ggcgtgatca caggatgttc
     2821 agatttgact ttgacactga ttttttcgta gcgaagtttt ggtaggatac attcgtagct
     2881 ccaacctaga atttcgccct ccttttgcca gcgagtgaca ttgacgactt ggtattcttt
     2941 ttcgagtgtt tcttttggta aactgagatc ggtaattttt attggcatca caattctcct
     3001 tttttatacg aaagtttata cgatttgatt aaaaaatata gatggccatc tacgacttgt
     3061 attatatacg agaacttata cgtagtcaag agattttcta aaaaaatata tacgattttt
     3121 tatacgagtg ttataatgaa aaaaatgata aggaggaatt tgaatatgtt tggtttagta
     3181 caacctaata aagtgaaggt gggccaacga atcaaagaaa taaaagacgg aatgtctctc
     3241 tcttttacag aattagggaa tcgattaggt ttaaaaaagc cgacgatcag ttcctacgtt
     3301 caaggctatg cgttggctcc taagagtgtg atcaaccaat tatctagtat cagcggaaaa
     3361 cctgtcggct ggttttattt tgggcatgtg gaagaatata tccgagagta tttgtttctc
     3421 attggtcaga aaaaaatagt ggacgattat cctgaaatca ttgagaaaat aaagcaagaa
     3481 ttttatacgg gcgagttcaa aaatcctgct tgggagaatg agctaggcta tcctttggag
     3541 gaatttatcc aagaatgttt tgtagaatat tcgagtgaaa tcatgaagga ctatgtcgta
     3601 cagattacga aagaggtact tgaagaatcc aaaacgctaa acaaactatc atcaaaagca
     3661 aaagaagaag ctgtcacgat tctttcatct gggatcattg actatatcga gatgagtggt
     3721 gagatcaagt atggagagaa agatcgcatt gcgaaaatgg tgaaagaatc aataaaaacc
     3781 tttgatgtga aacaagacct gacatttaat gattcctatt tgattggaaa gttgatcaat
     3841 attcttggta cagatgaaga tacggagctg tttctttctt ctttatcaat gatatttact
     3901 gataagcatt tttccactcg atttggtggg gaagaattag tcgaaatatt tcaatcgatg
     3961 cgaccagcgt taatcaaact ctatcgggag aaaacaccag atgaatttta tgattggttt
     4021 gagaagtaaa ataagaaaag tggcttagat tacaagttgg tgaaaaaatt tttttcagca
     4081 taacatcagt aatctataaa ggggttgttg attatactgt agaagttaag tactcaagag
     4141 attttttgta cgtaaacaaa gcaaaaaagg gtgcagtttc actagaaaat gtactctttt
     4201 ttgtgatttt tcttagttat agagtttttt tgaaacttta aaaatgagaa aacatattga
     4261 ttttaaagag tgtagacata gcttcttttg ctaaatagat taaacttact ggtgagtggt
     4321 cttgaacata cttttaaatc acattttact gagttttatt ggattttata attcattgga
     4381 aacctcgtac ttaatctgtt tttatctatc gactagaatc tgtatatatt ccagctatca
     4441 atgaggccgc agaaaagggt gcttaaaaat aatctgttgt ttaaaatatt attatcagaa
     4501 tacttagata caaaattctg gcacaaagta aaaaaaatat tgacttaaaa aaatggatat
     4561 ggtaatctat aaacgaaaat agtggtaacg tttccaaaat gcatcttcgt taaattttta
     4621 tttttttaaa cgaaaatgga aacgtttcta aaatgtattg ggaggagatt gaattggaaa
     4681 agacattggg accaggtggt gagataaggg cggtacaacg ggataaaaaa aatatttcga
     4741 agtgggaaaa attaaagaaa cagttgcggc gtgataagtg gatgtacttg ttgctcttac
     4801 caggtatcat catgacgttt atctttaagt atgtgccaat gtacggtgct gtgattgcat
     4861 ttaaagacta taatcctatg ctaggaattt tggaaagtcc ttgggtagga ctagatcact
     4921 ttagggaatt tatcagttca ccaaatttta cgcttttgct gagcaacaca ttaaaaatta
     4981 gtatttatgg tttattgtta ggtttttttc caccaattat tttagcactg agcttgaatc
     5041 aaattatcaa taaaaaaata aaacagaaaa tccagttgat tctatatgcg ccgaatttta
     5101 tctcagtagt tattattgtg gggatgctgt ttttgttcct atccgcaaat ggacctatta
     5161 atgggttagt agaaaaggtt acggggcaac caattatgtt catgtctaat ccagcttatt
     5221 tcagaacgat ttatatttta tcgggaattt ggcaaggaat cggctggtca tccattcttt
     5281 acaccgcaac gctggcggga gtcagtaccg atttatatga ggcagcaaat atcgatgggg
     5341 caaatatttt acaaaagatt tggcatatcg atttgcctgc attgaggcct gtgatggtga
     5401 tcagcttcat cttggccgcc ggcggtatta tgaatgtagg atatgaaaaa gcctacttga
     5461 tgcaaacttc aatgaacatc cctgcctcag aaatcatttc cacttatgta tacaaagtcg
     5521 gcttgcaatc aggtgattat gcctactctg ctgctgttgg tctgttcaat atgattatta
     5581 atgtcgtttt attactgatt gtcaacaata taaccaagcg gctaaacgat ggtcaaggct
     5641 tgtaatgaaa ggagaaaatt atgaatagtg ctttatcaaa aatcgaccgt caaatcttat
     5701 ggttgaatcg agtgattgta acttttttag tcgtcatcac cattgttccg ctgatttatg
     5761 tactggtagc ttcttttatg gacccagcta ctttgatcaa taaaggtata agcttcaatc
     5821 cgaaagattg gacaatacag ggatatcaac gggtatttgc agatgattcc attttacgcg
     5881 gttttgtaaa ttcgttgttt tactctttta gcttcagctt attgactgta tttattacga
     5941 tgatcactgc gtatcctttg tctcgaccta acttggcggg gaaaaagatt attatgactt
     6001 tttttgtcat tacaatgttc gtgggtggtg gcttgatacc tacctattta ttggtaaaga
     6061 acttgggcat gttagatagt gtatgggcga ttatcgtacc aggtgctata aatgtgtgga
     6121 atattatctt agcccgtacc tattttagtg ctttgccaaa agaactttct gaagctgcta
     6181 ctatcgatgg cgccaatgac ttgcaaatac tgttcaaagt catgcttcca ttagcgaaac
     6241 caattatgtt cgtattgttt ttatatgcct ttgttgggca atggaactca tactttgatg
     6301 ccatgattta catcaaagat cccgacttac agccgttgca actcgtatta agaaatattt
     6361 tgatccaaaa ccaaccttca caagacatgg tgggagccaa tactgccatg gcagaaatga
     6421 aacaaattgc tgagttgatc aaatacgcaa cgatcgtcat ttctagtctg ccattaatca
     6481 taatgtatcc tttcttccaa aaatattttg ataaaggtgt tttagttggt tctgttaaag
     6541 ggtaagtatt gttcactatt tgtatgaata aaaattataa aggagatttt aagatgaaag
     6601 caaaatggat gctgccaatc gtttttactt ccgtactaat tttgggggct tgtggaggct
     6661 caaaagaaaa gagtaaatca agccctgatt atgaactaga aaatgtctct tttccattga
     6721 aagaaaaagt atctttaaaa atgatgtctc aaagtgcccc tttggctccc aatgatccta
     6781 atgacaaatt gattttccaa cgattagaag aagagaccaa tctccatatt gattggacaa
     6841 actacagtag tgattttgcc gaaaaaagaa atttggatat cgctagtggt gatttaccag
     6901 atgccatctt caacgccgct gcaggtgact atgatttatt aaactgggcg gaaagtggcg
     6961 ttattgtacc gattgaagat ttgatcgaag attatatgcc aaatctgaag aaaatttttg
     7021 atgaaaaccc cgaataccga caaatgtcta ctgcacctga tggtcacatt tactctatgc
     7081 cttggattga agaattaggc gaaggaaaag agtcgattca tacagtaaat gggatggctt
     7141 ggatcaacaa ggagtggttg gataatttgg gtcttgaaat gccgcaaacc actgatcagt
     7201 tgatgactgt gttggaggct ttcaagacgc aagatccaaa tggtaatggt gaagcagatg
     7261 aaattccttt tagctttatt aacaatggag gaaacgaaga cttaaaattt ttaatggggg
     7321 cattcggtct aggtgataat gatgaccact tagtcgttga taacgatggt caggtacaat
     7381 ttactgctaa caaagaaggg tataaagaag ccatcaaata tttcaatgaa atgaataata
     7441 aaggtcttat tgataaagag gctttcgaac aagattggaa tacgtatgtt gccaaaggga
     7501 aagaacataa gtatggcgtg tactttactt gggataaagc aaatatcacg ggtgcagatg
     7561 atagttatga cgtcttgcca gtcttagcgg gaccagatgg aacgaaacat gttactcgta
     7621 cgaatggcat gggtttctca agagatcgtt tcgtaattac tagtgcgaat aaaaacttgg
     7681 aacttacagc taggtggata gataaaatgt atgaaccgtt acagtctgtc caaaataact
     7741 ggggaaccta cggtgatgaa gcacaggcga atatttttga attaaaggat ggtatgttaa
     7801 aacatttgcc tttagagggg acagcaccgg gggaactaag acaaaaaacc gaagctggag
     7861 gaccattagc agttcttgat tcttattacg gcacagtcac tactatgcca gatgatgcga
     7921 agtggcgttt agatttgatg catgacacgt atttgccata tattacagaa gacaatatct
     7981 atccacgtgt ctttttagaa aaagaagaaa cagatcgatt agcaaaaatc gaaacggata
     8041 tgtgggatta catctatcgc aaaagagcag agtggatcac cggcggtaaa atcgatgaag
     8101 aatgggaaga ataccttgct gaattggaac gtgtaggttt ggaagaatgg attcaaataa
     8161 aacaaactgg ctatgataat tttttgaagg gagcacaata agtgttagta gaagaaacgg
     8221 cgattttgga taaagcaaat cgatttattg aaatgatgcg aggaaaagtt aaccctcagt
     8281 atcgacaaaa ctatcatttt tccgcaccca tcgggtggat aaatgacccc aatgggttta
     8341 tatattacaa agggcattac catctcttct atcaatacta tccttacgat agtcgatggg
     8401 ggcccatgca ttgggggcat gcgcgcagta gtgatttggt ccattgggaa gatctaccag
     8461 tagcgttagc cccagatcaa ccttatgaca gcggcgggtg tttctcagga agtgcaattg
     8521 aaagagacgg aaaactatat ttattttata caggctgtca agaaattgat ggtattagcc
     8581 atcagcatca atgtttggcg gtctctgaag atggtattca ttttagcaaa tacagtggta
     8641 atcctgtggt agcagaaaaa cagctaggtg aaaatgggct aatacaagaa tttcgtgatc
     8701 ctaaagtaat tgttcacaac gatggttact atatggtagt agctactaaa tctacacagt
     8761 ctactggtag aatagtgatg tttcaatcgt cagatttgta tacttggcac ttttactctg
     8821 tgctgctaga agggacttca gagcaaggaa taatgtggga atgtcctgat ttattccatt
     8881 tagacggacg agatgtctta atcatgtctc cgatacaaat caagaagaac ggactggaat
     8941 atcataatat cagttctacc atggcttgta ttggtgaggt aaactgggaa acaggtcaga
     9001 tgatagttga taatttccat gaaatggatt ttggaatgga tttttatgca ccacaaacaa
     9061 caagagatcc taatggtgga cgggtgatga ttgcttggat gcagatgtgg gatcgtacat
     9121 tgcctacaca tgagttaggc catctttggg caggctctat gagtttgccc cgagagttaa
     9181 gagtgaaaaa aaatcgattg gtacaaaaac ccatctcaac tgtatatagt catttagttt
     9241 atcaacaagg cagtgaaaat attgcagtga ctgacacatc agtcgtattt cgaaacgcgc
     9301 tgtctgattc ttcatattta catctagtcg cagatttgga gaaagctgaa gtttttacaa
     9361 ttgaattact gcggtctaag acggattgtc tcaaactagc ctatgacact gctacacagg
     9421 tttttgaatt ggatcgtcag aaactaggaa gagagcttac aggggcggaa gaagaccctc
     9481 tgaatgtacg aaaagttgaa gtacctttga ttgataatca gctcgttctt gagattttta
     9541 gagatacctc atcaattgaa gtctttatta atgggttaga agttatgacg gctactttct
     9601 atgagctgga aaaagctcga gatatcttgt ttcattcgat cggagaaagt cgtattcaag
     9661 cttttgaaac agcgagtgtg aaataaatgt ttgattgctt aggcgatgaa tcaattattt
     9721 aataggtttt gaaggataat gtttttagaa aagaacagat agctagattt cccatggatt
     9781 tgctattagt attttgaaaa aattagtaat ttatgacaaa attcattata ctaaacttgt
     9841 gcagaaatgt gagggatcaa ctatgaaagc aacaatgaaa gatgtcgcaa aattagcagg
     9901 tgttggagta ggaactgttt cccgggtatt gaataacggt tctgttaaag cttctactag
     9961 agaaaaagtt gaagcagcaa tgacagagtt aaactatcaa ccagattttt atgcccgggg
    10021 gttaaagatg aagcatacga acacggttgc tttgattcta cccacggtat ggcatccatt
    10081 ttttggcgaa tttgcctacc atgtagaaac agcattagaa agacaaaact acaagcttta
    10141 tttgtgtaac tccagtggta atagtgaaaa agaacaagaa tatatccaga tggtcagcca
    10201 aaataaagta gacggcatca ttggaattac ttattcagat atcgatgaat acatttcgag
    10261 taatctgcct tttgttagta ttgaccgtta tttcactgaa gaggtgattt atgtgaccgc
    10321 tgacaatgcg gccggtggac gtttggctgc aaatgaactt tatcgccgag gatgtcagaa
    10381 tgtagcatat atcggtgggt accaagaaac accgaatgaa acgaagagtc gtcgaaaata
    10441 ttttgaaatc gagtgccgtc aactggggat ttcctataaa gcgttagata tgttagaacc
    10501 tttagaccat ctctcagaac gtgtaacaaa ttttttgtta gagaatcccg atatcgatgg
    10561 gatttttacg atcaacgatc caatggcatt agctgtcatt gatatgcttg aaaaaatagg
    10621 gaaaaacgta ccagaggatg tccaagttat cggatttgat ggtcaaaaga tgacagaagg
    10681 agatccttat ctagtctcaa cgattgccca acctacaaag caaatggcgg aagtggcagt
    10741 ggcaaaatta attgctgtca ttaatggaga gacagtagaa aaacgtaccg tattgccgat
    10801 tgaatttggc gaaggtgaca caacgaaacc tttagtgaga aacgattttg cttagtgcaa
    10861 atacgtttct ttttttgaaa atgagtgaat gaaaggtgta gataaaatgt tatatggtgg
    10921 aatagaagcc ggtggaacaa agtttgtctg tgggattggt aatgaacaat tacaagtgat
    10981 agaacggaaa agttttccta ccacaacacc agaagaaacg atggagcaag tgtttgattt
    11041 ctttgataag tacaaaggaa atatcgcatc aattggtgta ggagcatttg ggcctattga
    11101 tattcaaatc gaatcaagaa cttacggata tatcaccaac acaccgaagt tagcttggca
    11161 aaattatcat tttgtaggag ctttggaaaa cgccttgtcg gttcccatct attggactac
    11221 cgatgtaaat gctgcttgtt atggagaata tatggcaggg tatggaaaag gtaaaaatag
    11281 tgttctgtat tacaccgttg gtacaggtgt tggtggtggt gctgtagtgg atgggaaaat
    11341 agtcgcaggt tttagtcatc ctgaaatggg acatatgctc ttacaacgtc ataatgaaga
    11401 tcaatttgaa ggaatctgtc cttttcatca tgattgtttg gaagggttag catcaggacc
    11461 tgcgattgaa aaacgctttg gaagaaaagc ccagactatc tcatttgatc atccttattg
    11521 gacgattgag gcagattatc ttgctcagtg tgtgtataac acaacgttac tatatgcacc
    11581 ggaagtaata attttaggcg ggggtgtaat gaaacagccg caactactcg agttgattcg
    11641 ccaatcgttt aaagaaaaaa tgggttccta cgtggatacg cctactctcg aagaatatct
    11701 actaactcca aggcttgctg acaatgcggg cttgattggc tgcctagctt tagcaataaa
    11761 gaaactcaat cattagaata gtatttatta gaactattca taacataccg gaagtttaaa
    11821 agggtattct gaaacttaaa tagtctcaat tcattcttct ttcttgagaa tctgagtaga
    11881 gacttttatt ctgtttcaaa ttaatataat tacgaaatga tattgagtac aagaaagttc
    11941 tttttaccag atcttttaaa tcgaaaaatc tgatatatat atttatagta ctaaaaatct
    12001 gattagataa aaagctaatt caataggaaa ataaaggtag tgaataaaag caaagccctt
    12061 gttagtagtt aatctaacaa gggctttggt agggaaaatg gtaggaaacc atcttatttt
    12121 ttacgaaaat caggaacttt agtaattgaa taatgttgat ttataggtat tcctcatcca
    12181 tattatgttg gctctttatt cccactccac tgttgcaggt ggtttgctgg taatatcata
    12241 cacgatacgg ttaacgtgtg cgacttcatt tacgatacgg acactgattt tttgcaagac
    12301 ttcccaaggg atacgggcaa agtcagcggt catgccgtca atagaagtga cagcacggat
    12361 acctacagtg tagtcatacg tacggccatc tcccataacc ccaacagagc gaatgcctgg
    12421 taggacggtg aagtattgcc agatgtcgcg atctaaaccg gcattggcga tttcttcgcg
    12481 caagatagcg tctgattcac ggacgatttc taatttttct tctgtcaatt caccaaggac
    12541 acgaatacct aagcctggtc cagggaatgg ttggcgccaa acgatgctgt caggcatgcc
    12601 taattgggta cctaattcac gtacttcgtc tttgaacaat gtgttcaatg gctcgatcag
    12661 cttgaattgc atgtcttcag gtaatccacc tacattgtga tgtgatttga tggtttgggc
    12721 agtttcagtt cctgattcga tcacatcggt ataaagggtt ccttgtgcaa ggaaagaaac
    12781 gccttcttca ccggcaagtt tggttgcttc atcatcgaag acatatacaa attcatttcc
    12841 aatgatttta cgtttttctt ctggatcaga cacgccagca agttttgata agaaacgttc
    12901 tttggcatcg acttttataa tgttcaagcc aaattttccg ccaagacttt ccataacttg
    12961 ttctgcttca cctttacgca acaatacgtg atcaacgaag atacatgtta attgatcgcc
    13021 gatcgctttt tgtaaaagaa caccaacaac agaggaatct actccgcctg aaaggcctaa
    13081 taaaactttt ttgtcaccga cttgttcacg aatttttgcg atctccatgt cgatgaagtt
    13141 atccattgtc cagtcaccag tacattggca gacatctagt gtaaaatggc gcaacaagtc
    13201 atttccatat tcggaatggc gaacttctgc atggaattgg attccgtaca ttttgcgttc
    13261 tgcattttgg atcgatgcga tggggcaatc tttgctggta gcaacgacat cgaaatcagt
    13321 cggtgcttgg gtaactaggt caccatgact catccaaacg gtttgttttt caggagtgtt
    13381 ttggaaaagg gccgcttctg cagaagtgat ttccaattct gcttttccgt attcacggtt
    13441 tttggctggt ttaacttttc cgcctaattt ataagtaatc aactgcatac cgtaacagat
    13501 tcctaaaact gggatcccca aatcaaaaat cttttcatca ataccaaagg ccttctcatc
    13561 ataaacactg tttggtccgc cagaaaggat gattccttta gggttcattg cctttacttc
    13621 ttctgcggta atgcggtggc tcatcaactc tgagaaaaca ccgaactcac gaatacggcg
    13681 agtgatcagt tggttatact gactaccgta atcaagtacg ataatttttg gctcttcgtc
    13741 aaatcgtgtt ggtgaagaca aatttgaata aaacttagtc cgatatgcgg cgcgttctgt
    13801 gcgccgcata tttttttagt tttcaaagat cagacct
//
LOCUS       DACGLH010000073        13807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.078, whole genome shotgun sequence.
ACCESSION   DACGLH010000073 DACGLH010000000
VERSION     DACGLH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.078"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXT21_002244"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXT21_002244"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ4869623.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IXT21_002245"
     CDS             complement(2260..2511)
                     /locus_tag="IXT21_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ4869624.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IXT21_002246"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IXT21_002246"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ4869625.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IXT21_002247"
     CDS             3461..3679
                     /locus_tag="IXT21_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869626.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IXT21_002248"
     CDS             complement(3968..4480)
                     /locus_tag="IXT21_002248"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ4869627.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXT21_002249"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXT21_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ4869628.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IXT21_002250"
     CDS             complement(6933..7367)
                     /locus_tag="IXT21_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ4869629.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IXT21_002251"
     CDS             complement(7377..8165)
                     /locus_tag="IXT21_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4869630.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IXT21_002252"
     CDS             complement(8162..9103)
                     /locus_tag="IXT21_002252"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ4869631.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IXT21_002253"
     CDS             complement(9280..9867)
                     /locus_tag="IXT21_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ4869632.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXT21_002254"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXT21_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ4869633.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IXT21_002255"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IXT21_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ4869634.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IXT21_002256"
     CDS             complement(12784..13719)
                     /locus_tag="IXT21_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4869635.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DACGLH010000074        13747 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.079, whole genome shotgun sequence.
ACCESSION   DACGLH010000074 DACGLH010000000
VERSION     DACGLH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.079"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(96..1661)
                     /locus_tag="IXT21_002257"
     CDS             complement(96..1661)
                     /locus_tag="IXT21_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ4869636.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(1654..3105)
                     /locus_tag="IXT21_002258"
     CDS             complement(1654..3105)
                     /locus_tag="IXT21_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4869637.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(3119..4135)
                     /locus_tag="IXT21_002259"
     CDS             complement(3119..4135)
                     /locus_tag="IXT21_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4869638.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            4356..5444
                     /locus_tag="IXT21_002260"
     CDS             4356..5444
                     /locus_tag="IXT21_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4869639.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            5498..7024
                     /locus_tag="IXT21_002261"
     CDS             5498..7024
                     /locus_tag="IXT21_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869640.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            7026..8231
                     /locus_tag="IXT21_002262"
     CDS             7026..8231
                     /locus_tag="IXT21_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4869641.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            8488..9465
                     /gene="guaC"
                     /locus_tag="IXT21_002263"
     CDS             8488..9465
                     /gene="guaC"
                     /locus_tag="IXT21_002263"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ4869642.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            9729..11153
                     /locus_tag="IXT21_002264"
     CDS             9729..11153
                     /locus_tag="IXT21_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ4869643.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            11614..12723
                     /gene="pdhA"
                     /locus_tag="IXT21_002265"
     CDS             11614..12723
                     /gene="pdhA"
                     /locus_tag="IXT21_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ4869644.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            12726..13703
                     /locus_tag="IXT21_002266"
     CDS             12726..13703
                     /locus_tag="IXT21_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ4869645.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         4385 a         2353 c         2971 g         4038 t
ORIGIN      
        1 aaagacagaa taacttgaaa gctgttttca gcttcgagat attctgtctt tttttattgc
       61 aaatggaaag agtatcattg tacggatttc gtttattagt tatttttgtt tttaaggaaa
      121 agctgtgtcc gatattcttt tggagaaatc ccttgttttt tcttaaataa ataactgaaa
      181 tagttagggt cattataacc aatttcaaaa gcaatatctg aaatacgata gtctgattgc
      241 tgcaataatt tttttgctaa ttcgattctt tgttctgtca gataatcgat gaatgtttgg
      301 ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg
      361 acccaagtta aggacatatc tggatcggta aaattttctt tgataaaatc caatgcatgg
      421 ttcaaaattg gctgatattt gttccttgaa ggctgtgagg gcagagcagt taagaaagaa
      481 atgaacatat aaactgtcga acggtaaagc gagtcgtcgg cgctgattgc taagcgttga
      541 ctggtggatg tgcaatgaac catttcttgc gggattttct tttctttcag gcagacatgc
      601 tggagtagct tagtcaagac aaataaacga tataatcgtg ttcgagttgg tgtgccttgt
      661 ggtttcgtca agtgttcgat gtacttagat acttcgtttt tcgataatga agacaaatca
      721 agtgagaggt caaagtaaaa gacagaatca actggaagtt tcgcttcttg aatcaaatcc
      781 tcatagctga taatttgttc tttaagggaa ctggcggttt caagtatcat ctgcgtgatc
      841 tcaaaagatg caggtaattc gctgattctt tctgcgacag gaccaatggc gacagaaaaa
      901 ttattttttt gtcccttttc taattcatgg accaacgttt gggctacctg ataactggtc
      961 tcgatcacat tttcttcaag agaatcaact attaaaaatt tgatgaatcg agaggaaaca
     1021 gcagagaaga tcacttgttg attctcccta aataaaaact tcaaataatc actgagatga
     1081 gcgtaatctt caaagtctgt actgacttgg tttgctgcta ataatacggt catttttttc
     1141 cctgctaaag agcgattgaa agattcagca agtttcaatg tttcactgat cgtaagtgag
     1201 cctttgaaca aaccgttcaa gaaatgattt tttttcaatt cgaaaacaag atcagaagaa
     1261 gtgtgcgttt ctttttgctg caaatctaaa gcagttactg ctttttctat tgcttgtttc
     1321 aattcgctac ttttaatcgg tttaagcaga taatcatctg cctgtacctg gattgctcct
     1381 ttcatataat cgaaatcgtc gaatccacta ataaatatga gacgagtcca aggaagtagt
     1441 ttccttgctt cttttgaaaa agtcaacccg tccatgaaag gcatttggat atcagtcaga
     1501 atgatgtcgg gctgtaagtc gataattgaa gacagtgcca tctcaccatc tccagcttcg
     1561 ccgacataag tgattggata ataagaacca aggacagtta tttgttcccg caagttattg
     1621 cgaatgaggt attcgtcttc gatgataaaa actttataca tgtttgtttc ctccaattgt
     1681 tttgggagat agagggatca tcagtgtgac agttgttcct tctcggtatt tgctgtcaat
     1741 cgtgataggt ttttgatcat ttccgtaata aagaaggaga cgcttgttta cgttgtaaag
     1801 tccgtagccg atactgaaat ctgaatcgat ccctttggct aactctttac gtatttctgc
     1861 cagtttttct gcagggatgc caatcccgtt gtctataact ttgaattgga tcatctcatc
     1921 aattttgcat gtggtaatac gaataaatcc tttgtgacgg acgtacttcg ttccatggta
     1981 tacagcattt tcaatcaatg gctgcaagat catttttaaa atagaaacag gaaaaagtgt
     2041 ttctggtata tcgatcttat agtcaagcat atggccgtat cttattttta agatgactag
     2101 ataatcattg atatggctaa tttctttttc aagaggaatc cagtcttgtc ctttgcttaa
     2161 agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc agagcttctt caaagcagtc
     2221 ttgttcgatc aaagaaataa tggcatccaa actgttataa ataaaatggg gagtaatctg
     2281 agcttgcaat acacgaactt cactttgtgc taaatggtat tgtttcaaag cgttctcccg
     2341 taaagactgg tttagatctt ccgccatttt attcatgcta gcggtaagtg tttgtaattc
     2401 cagtgtttcc ggcagggtta aacgaaagcc aaggttccct gaagaaagtt gttctgacat
     2461 ctcgatcagc tgattgatcg gttcttggac ctttctctct aaaaagcgac gattcttttt
     2521 cgcatagaaa aaaatggtac taagaactcc aagttccact attgttaata gaatgagcga
     2581 gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc tccacacgaa caaaatcttg
     2641 taagatatct gacagcagtt gagtgacaga atctacctga ttgatgatct cttcattggt
     2701 atccataaaa tcttttgcca agatattatt gatcagctcg ttttgataat cttccagtgt
     2761 atcgatcgta cgtagcgata catctaacgt actaatttct ttaagtgtat ttgtattatt
     2821 tttgatctct cgaatatcat tcctaagttc ttctaggata ttttcctcat tgaactcatc
     2881 tacggttact tggccagaaa tcaagtccca catactttcc aaaacttttt gatcaacctt
     2941 caaagcaatt gcatctgctt tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg
     3001 ttgcgaatat aacagtatgc tcagtagcaa agggataaca gcaaatagaa gcatggcttt
     3061 gtttgcttgg gcaaaaatct gttttatgct taaatttttt gtcatgatac ttctccagtc
     3121 aataatttct ggttgggatg ttagctatat tctgctggga aaagacagtc tcggaaataa
     3181 aaatatcttt aggtaatgtt tttcctgttt gaatatttcc aaaataacga gtaacggtat
     3241 tggctacata ccagccgaca tcaggtttac actcgactac acaattgacg attcctgagc
     3301 gtagcttctc gatcatatct tcttgtccat caatagttac aatgatcaaa tcttctccag
     3361 gtttgattga tgtttctttt atcgcagcta aagcaccgtg agtcatttca tcactatggc
     3421 tgtataatac atcgatttcg gataaatcat ttgtttctaa atatcgtttc atctggtctt
     3481 ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat
     3541 ggtcgatggt tccatctttt tttagggttg gtttacgatt gattgattcc aaaaatcctt
     3601 cggagcgtag cttggtcggt gtggagttct ctgaaccagc aagttctagg attctgattt
     3661 cctgtttctt tgaatttgtg aagtgattgc tgacaaataa acccgcccga tttccttctg
     3721 ctttgaaact ggggccaatg tgggtgacga aaagctcggg atcattcgtt tgaatatgtc
     3781 tgtcgacaag tatgacaggg atccctgctc gctttgcttc ttttaataca gagtcccacc
     3841 cggattcttg caaaggagta aaaatgatca tatccacttt ataggcaatg aacgtccgta
     3901 tatcttggat ttgccgttcc tgattcatgt agccatttcg gtagagaact tggtaatctt
     3961 ctttttcaag cgcagtgata attgattcag tatgcttttt ccgccagcta ctctccgttc
     4021 ctgattgcga aaaaccaacg actagtgggt caggtgcttc ctttgtatta ggtgtacacc
     4081 ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa
     4141 atcactccat ctggatttgt tagtactatc ttatcagata aaagggaaaa tcaaagtcaa
     4201 aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac
     4261 ctaaaaaatt taaagtaaat cccataaaaa aaatcaatgt taacgctttc tttattagct
     4321 atactcggtg tatcaattga aatgggggat aaaaaatgaa aaacaaattt agaaagattg
     4381 ttgggactat cgctattttg accgcaagca ttctattaag tgcgtgtggc aattcaggat
     4441 ctgcagatga tagtacgggg tatgtaggaa tcgctatgcc gaccaaatca gcagaaagat
     4501 ggatcgctga tgggaacaac atggtctcgg aattagaaaa actgggttat aaaacagatc
     4561 ttcaatatgg agaagataaa gtagaaaatc aagtagcaca aatagaaaat atgattacaa
     4621 aaggggtaga tacacttgtg attgcttcta tcgatggttc ggctttaact gatgtgttag
     4681 ctaaagcaaa agaagcagat atcaaggtga tcgcctatga tcgtttactg atgaattcag
     4741 aaaatgtcga ttattacgcg acttttgata attttggagt aggtgtatcg caagcagctt
     4801 atattgaaga gcacttagga ttgaaagaag gaaaaggacc attcacgatt gaattatttg
     4861 gtggctcgcc tgacgacaac aacgcactaa tcaattacaa cggagtgatg tctgttttac
     4921 aaccatatat ggacaacggt cagttggtcg tgccatctgg acagacgagt ttcagtcaaa
     4981 tcgctacgct tcgctgggat ggttctacgg cgcaagcaag gatggataat ctgttaagcg
     5041 caaattatac tgaccaaaca ttggatgctg ttttgtcgcc atatgatcca ataagtttag
     5101 gaatcatttc gtcattaaaa ggcgtggggt atgggagtga aagtaaacct ttacctgtga
     5161 ttactggaca agatgcgaca gttgctggcg ttaaatcgat cattgccggg gaacaaacac
     5221 aaacgatctt taaagacaca cgtatcttag caaaaaatac gattgaaatg atcaaagcaa
     5281 tcagtgatgg agaagaggtt ccggtcaatg atactgaaac ttatgataat ggggtgaaaa
     5341 ctgtccctac gtatttagca aatacagtat ctgtcgataa agataattat caagcagagc
     5401 tgatcgacac cgattattat aaagaatccg atctgaaaaa ttaagaggaa ccaaacaagg
     5461 aactaaagag atctaagtca gagagaggat aagaattatg gcggattgta ttttaaaaat
     5521 ggcagatatc accaaaaaat tttctggaat ccgagcgtta gattgcgtga atctgatgat
     5581 tgaacgtggg gaaatccatg cattgtgcgg agaaaacgga gcgggaaaat caacattgat
     5641 gaatgtcttg agcggcttgt atccatatgg ctcctatgac ggggaaatct tttatgatgg
     5701 aaagctgtgt caatttaaag atttgaaaga tagtgaagaa aaaggcatcg tcatcattca
     5761 tcaagaatta gcactaagtc cttatctttc aataaaagag aatatttttc tcggaaatga
     5821 acaagcagaa agaggcgtga ttgattggga actgacagaa aagaaaacat atgaactgct
     5881 gaataaagtc ggtttgtcta tcgacccgaa tcttctagta tcccagattg gcgtagggca
     5941 acagcaacta gttgaaatag ctaaagcatt atcaaaatcc gtccgtctgt tgattttgga
     6001 tgagcctacc gctgcattga atgaagaaga aagcgcgaat ttgctgagat tgatccgaga
     6061 gttgaaagag caaggtgtaa cctccatcat tatttcacat aagctgaatg aaatcgtgga
     6121 tatcgcagat cgaatcacca ttttacggga tggacagacg attgagacac tagaaaatga
     6181 aatgatcagc gaagagcata ttattcgcgg aatggtgggc agagatttag ttgatcgtta
     6241 tcctgaaaga aaaccggata ttggagaagt ttattttaaa gtagaagact ggacggtcta
     6301 tcaccctctt gatcacgagc gaatcataaa cgaccacatc aacttgcatt tgaaaaaagg
     6361 agagattgta ggaatagctg gactgatggg agcgggcaga actgaattcg ctatgagtct
     6421 atttggacat tcttatggaa gcaagatatc cggacgtata ttaaaggaag gggcagagat
     6481 atcagttaag accgttcctt cagccattgc aaacggactc gcttatgtct cggaggaccg
     6541 aaaatcttta ggactgaatc tgctgatgga tattcgggaa aacgtgacgc tgagcagttt
     6601 ggataaaata agtcaaaaag gagtattaga taaagaaaaa gaagtaactg aggcagaaaa
     6661 tttccggaaa aagatgcgta taaaaacaaa ctccatccat caaattgtca gtagtcttag
     6721 tggtgggaat caacaaaaag tcgttttatc caagtggctg atgacacaac ccgatattct
     6781 ttttttggat gagccaaccc gtgggataga tgttggggca aaatatgaga tctacacgat
     6841 tatcgaagaa atggcagcaa atggcaaagc tgtctgtctt atctcttctg aacttcctga
     6901 aatattaggg atgtgtgatc gaatctatac gatgaacgaa ggacgtatta caggagaagt
     6961 ctcgcgagaa gatgcagatc aggaagtctt gatgaaatta atgacgaaag aagaggtggg
     7021 ttaagttgga acaggcaaaa aatcttacgg ataaaaaaga gaaaaaacta aatttgaaaa
     7081 gtagagcagc tgatcttttt ggtcgctaca gcatggtgat cattttagcg ggactactta
     7141 ttgcgtttca attgatgacg gatggtattt tctggcgtcc attgaatata actaaccttg
     7201 ttctgcaaaa tagccatata ttagtactag ctgcagggat gttattagtt gtgctcttag
     7261 gtcatgtgga tttgtcggta gggtcagtga tggcgtttgt tggtgcaatt gcgggcatga
     7321 tgatggtcaa tcgccaaatg aacccgtgga tgacagttat cgcatgtctt gcagtaggcg
     7381 ggctgatcgg cgcatggcaa ggtttttggg tagcttatgc agggataccg gcgtttatcg
     7441 tgactttagc aggattgctg atgtttcgtg gactgacaca agttgttcta ggaggccaat
     7501 ctctagcacc ttttccagct gttttccaaa aaatgtctac gggttattta ccagacttaa
     7561 gcgaaggaag cagccttcac ttcttgacat tgattttagg aatgatcatc acgatcgtgt
     7621 tagttgttgg tcaatggcga acaagaaatc gtcggaaaaa tcatttattt gaagtggaat
     7681 ctccaacagc tttcttggta aaggcggtat ttacagcagt cgttgtacta ggaataactt
     7741 atatctttgc ctcttatcaa gggtatccga ttattttgat cattcttggt ttgatcgttg
     7801 cagcatatgg gtttttgaca aataaaacag tagcaggtcg acagatttat gcgacgggcg
     7861 gaaatcgaaa agcggctgaa ctatcaggga tcaaaacaaa gcgaatcacg ttctgggtat
     7921 tcgtcaacat gggagtcatg gcagctttag ctggattagt tttagctgct cgattgaatg
     7981 cggcaacacc tcaagcaggt acatcgatgg aactggatgc aatggcggca gtatattttg
     8041 gtggtgcttc gacttccggc ggcattggaa caatcatggg tgcgattgtc ggtggcttgg
     8101 tcatgggtgt attgaacaat ggcatgtcga ttttaggcgt cggggtagac tggcagcagg
     8161 caatcaaagg attgatcttg cttttagccg ttgtgctaga cttgtataat aagaaaaaac
     8221 gaacaacgta ggagaacgcc ggttattcag aaaaaagact gtgacaagac atttgtcaca
     8281 gtcttttttt cgctaaaagc gaacattcat tgtttcgttt gtatagacta aaaataatat
     8341 atgttttttt accattcgta taaattctct ttttgctctt gatttttttg cttaattgag
     8401 ttaaattaca tagggcgcta aaaaaagcct tgattaaatt aataatgttc gtgttttgct
     8461 ggatgtgaac atcaggagga taatgtaatg aaagtatttg attacgaaga tattcaatta
     8521 attcctaata aatgtatcgt aaatagccgt tctgaatgtg atacaacggt cacattaggt
     8581 aaacatacat tcaaaatgcc agtagtgcct gccaacatgc agacgattat tgatgaaaca
     8641 atcgcagaat tcttagcgga aaatggttat ttctatatca tgcatcggtt tgatgaagca
     8701 gctcgaattc cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc gatcagtgtt
     8761 ggcgtcaaga aagaggaata ttcctttata gagaagttgg cagaagaatc gctaaatcct
     8821 gattatatca ctatcgacat cgcgcatgga catgctaact cagttattga tatgattcag
     8881 catattaaaa aatatttgcc tgaaactttt gtgattgccg gtaatgttgg tacaccggaa
     8941 gccgtccgtg aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat tggtccagga
     9001 aaagtatgta ttacaaaaat caagacaggc tttgggactg gaggctggca actggcagct
     9061 cttagatggt gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg gatcagaaca
     9121 catggcgata ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat tggttcattg
     9181 tttgccggtc acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat cgtgtataaa
     9241 gaatactttg gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt tgaaggaaag
     9301 aaaatctgga tccaacacaa aggatctttg aaagatacac tagtggaaat gcaacaagac
     9361 cttcaatcat ctatttctta tgctggagga agagatctgg aagccattcg aaaagttgac
     9421 tatgtcatcg tgaaaaattc gatttttaat ggagacacaa tctaaaaaat aaataaaata
     9481 ttaggatgtt gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa acaaaaatag
     9541 ttctttatat gttttgtttg gctggaatta ttatcgcaga tacaagcttt tgaacatcat
     9601 tattcagaat aagggactgt gataagattt ttgtcacagt cccttatgtt ttgcaaaaaa
     9661 agtttttctt ttttctcgta aattcggtaa aatcaaaaga aaaaatagac aatagaaaag
     9721 agaagcttat gtgggaaaaa gtagagaaaa aacaaggacc gatttatcgt caaattatgg
     9781 aacagattat gagagaaatc gaaaacggaa ggatcaatcc tggaagtcaa cttgaatcag
     9841 aacgaaaact agggcaactt tttggtgtga atcgttccac cgttgtccat gcattggaag
     9901 agcttaggga gcttggggta ctaacgagca aacgtggcag cggatggttt gttaatcaga
     9961 ctgaatgggg gaaattcgct gttccaagaa tcgattggcg gcaaatgatc tctcctcgtt
    10021 atgagcagac ggactggtat gagcaaaaaa taagagaagc aaagcagatc caaaaaacat
    10081 cgtttcttga tttatacgct agcgagatgc cagatgagct tttgccgaat tttgagtttc
    10141 cttcttattc tcttgaagaa atcttagcgg aagaaaaaga aatggatctt ttaggctata
    10201 ctccgttacg taacaaaatc aaacggtatt tggaaagtaa atttgaattg gcattttccc
    10261 ccaaccagct tcttgtgact ggtggcggtc agcaagcaat ctttttgatt ttacaaacga
    10321 ttctttcagc tggagagggg atagctgtag agtcaccttc ttttttttac cgattacctc
    10381 ttttcaaagc ttcaggaacg cgtttatttg ggataccgat ggatgaagaa gggatcgact
    10441 tagaaaaact gaaagaatcg atccataaaa ataaattgaa agcagtgctt atcaacccga
    10501 attttcaaaa ccctacagga aaagtgatga gtcaaaaacg acgagaaaag ctagtagaat
    10561 tgtgtcgaca gtatcggctt ccaattattg aagacgatgt atttacggat ctttcatttc
    10621 taaaccaaga agaagtcctt cctatacgaa cgattgatcc ggataatgtg ttatacgtcg
    10681 gttcgttatc tagggtttta ggaaaaacaa cgaaaatcgg ttgggtgata ggacctgaga
    10741 cttttatctc gcaactagct caagcgcaag aaatgatgga gttttcgatg aatatcctta
    10801 cacaaatcac agtggctaat gtatttaatg aagcaattga tttgaaaatg caagatgtaa
    10861 gaaaaaagtt gaaagaaaaa agccgactga taagcgcttg gggagattca caatcgtttt
    10921 tcaagcttca ttcaatttta ggtggttact atgcctggat tacttgggat gggaaaaaac
    10981 tagaccccga actagcagaa aaagtagtag atatgggtct tggaattgca ccaagtctac
    11041 tttttgggca agatataaat ggaatcagaa tcaattacag taggctggat gaaaaccaaa
    11101 taccgttgtt ttattcaaag atgaaaattc tagaaaagtg gttaacagaa taatttcaca
    11161 aagtaaatag attaaagaat ataacagctt ggatttaaat ttcaagctgt ttcttttttt
    11221 tcggaaaaag aatacgatat aaagaaacta agaaaaaacc attatttgta cgtccgttca
    11281 cgatgtgtaa ataaaataaa tgtttgcaac aaaactgtat aacaccaacg tttttaaata
    11341 gaaacagatg gttgtaagtt tgattagaaa atagaaaaaa tgcagatgaa atataaaacg
    11401 ctttccttat tgtgatttta ttcactaatg ctgttaatat gcggattggc ataatttcct
    11461 ttcatcgtga aaatttgtgc ttttagcttg tatttctcat aaatccatga taatttatta
    11521 atgaagacgg ttaaatagtg atacagtttt attcactgtt tcaatcgcag aatgaagaga
    11581 ttttcttata gatgagagag gaatgtgtga caaatggcaa acaaaaaagc accgatcgac
    11641 ttccaagcac tgctagatga agtcaatgca gatttcccag tatatcaagc acttgatcaa
    11701 gatggaaacg tagttaacaa agatttagta cctgatttat ccgatgatga actcgttgaa
    11761 ttaatgactc atatggtatg gtctcgtgta ttagaccaac gttcaacagc gctgaaccgc
    11821 caaggacgcc taggcttttt tgcaccaact gctggacaag aggcaagtca gctggctagt
    11881 gcttacgcat ttgataaaga agatgttctt cttccaggtt atcgggatgt acctcaacta
    11941 gtaaaacatg gtttaccttt atctcaagct tttttatggt ctcgaggaca tgctgcaggt
    12001 aattcttatc cggaagaatt gaaagctcta ccaccacaaa ttatcatcgg tgcacaatat
    12061 gtacaagcag ccggtgttgc attaggtttg aagaaacgaa acaagaaaaa tgtcgtcttc
    12121 acttatactg gtgatggcgg ttcatcacaa ggtgattttt acgaagcaat caatttcgct
    12181 ggcgcttacc atgcaaatgc cgtattttat atccaaaaca acgggtatgc gatctctaca
    12241 cctcgtgaaa aacaaactgc agcaaaaaca cttgcacaaa aagctgtcgc agctgggatt
    12301 cctggtattc aagtcgatgg aatggatccg cttgcagtat atactgtatc taaaatggca
    12361 cgggaatggg cgatcagtgg aaatggacca gtcctaatcg aaacactgac ttatcgttat
    12421 ggaccacata cactatctgg tgacgatcca acccgctacc gtacaaaaga cacagatgac
    12481 gattggcaga aaaaagatcc attgatccgc ttccgtaaat atttgacgga aaaaggactt
    12541 tggtctgaag aaaaagaaga acaagtcatc gaagcaacta aagaagaaat caaagcagct
    12601 attgctgaag ccgacaaagt accaaaacaa aaggtttcag atttcttgaa aaacatgttc
    12661 gaagtatctc cacaatcaat caaagaacaa attgcaattt atgaagcgaa ggagtcgaaa
    12721 taatcatggc acaaaaaaca atgatccaag caatcacaga tgccttagca cttgaactag
    12781 aaaatgacga gaatgtcgtt gtttttggtg aagatgttgg gaaaaacgga ggagttttcc
    12841 gggcaactga aggattgcag gaaaagttcg gagaagaccg agtattcgat actcctttag
    12901 ctgaatctgg aatcgcagga ttgagtttcg gtcttgcgtt agaaggattt cgtccagttc
    12961 ctgaaatcca attctttggt tttattttcg aagcaatgga cgaagtagta gctcaaatgg
    13021 ctcgtacaag atatcgaatg agcggcacta gaaatctacc catcacaatt cgttcgccat
    13081 ttggtggcgg tgttcataca ccagaattgc attcggataa tttagaaggt ttgatcgctc
    13141 agtctcctgg tattcgagta gtgattcctt ccaaccctta tgatgcaaaa ggactattga
    13201 tttcttctat tagaagtaac gatccggttg ttttcctaga acacatgaaa ctatatcgct
    13261 cattccgaga agaagtgccg gatgaagctt acgaagttcc tttggataaa gcagcagtta
    13321 ctcgtgaggg aacagatgta tctattatca catacggagc aatggtgcgt gaagcaatca
    13381 aagcagcaga caatcttgca aaagaaaata tatctgttga aatcattgat cttcgtacag
    13441 ttgcgccatt agatgtagaa acgatcattc aatccgtgga aaaaactggt cgcgtagttg
    13501 ttgtccaaga agcacaacga caagcgggcg ttgccgcgca agtagtttct gagatctcag
    13561 aacgcgctat cctttcatta gaagccccaa tcggacgagt ttctgcacct gatacagtct
    13621 tcccatttgg acaagcagaa aatgtctggt tgccaaatgc aaaagatatc gaagacaaag
    13681 taaaagaaat cgcggaattt taagaaaaga agggacgaaa caaagtcatg gcttatcaat
    13741 ttaaatt
//
LOCUS       DACGLH010000075        13731 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.080, whole genome shotgun sequence.
ACCESSION   DACGLH010000075 DACGLH010000000
VERSION     DACGLH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.080"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(63..1268)
                     /locus_tag="IXT21_002267"
     CDS             complement(63..1268)
                     /locus_tag="IXT21_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ4869646.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IXT21_002268"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IXT21_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IXT21_002269"
     CDS             complement(1447..2832)
                     /locus_tag="IXT21_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ4869647.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IXT21_002270"
     CDS             complement(2861..3247)
                     /locus_tag="IXT21_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4869648.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IXT21_002271"
     CDS             complement(3263..3577)
                     /locus_tag="IXT21_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4869649.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXT21_002272"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXT21_002272"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ4869650.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IXT21_002273"
     CDS             4837..6126
                     /locus_tag="IXT21_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ4869651.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="IXT21_002274"
     CDS             complement(6165..6770)
                     /locus_tag="IXT21_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ4869652.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="IXT21_002275"
     CDS             complement(6966..7430)
                     /locus_tag="IXT21_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4869653.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="IXT21_002276"
     CDS             complement(7452..7772)
                     /locus_tag="IXT21_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ4869654.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IXT21_002277"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IXT21_002277"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ4869655.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="IXT21_002278"
     CDS             9194..9544
                     /locus_tag="IXT21_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4869656.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="IXT21_002279"
     CDS             9729..10682
                     /locus_tag="IXT21_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4869657.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="IXT21_002280"
     CDS             10695..12482
                     /locus_tag="IXT21_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869658.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="IXT21_002281"
     CDS             12469..13671
                     /locus_tag="IXT21_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869659.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3937 a         2689 c         2519 g         4586 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg g
//
LOCUS       DACGLH010000076        13700 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.081, whole genome shotgun sequence.
ACCESSION   DACGLH010000076 DACGLH010000000
VERSION     DACGLH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.081"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(24..359)
                     /locus_tag="IXT21_002282"
     CDS             complement(24..359)
                     /locus_tag="IXT21_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4869660.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            645..2525
                     /locus_tag="IXT21_002283"
     CDS             645..2525
                     /locus_tag="IXT21_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ4869661.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2604..2831
                     /locus_tag="IXT21_002284"
     CDS             2604..2831
                     /locus_tag="IXT21_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ4869662.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2836..3378
                     /locus_tag="IXT21_002285"
     CDS             2836..3378
                     /locus_tag="IXT21_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4869663.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3629..4306
                     /locus_tag="IXT21_002286"
     CDS             3629..4306
                     /locus_tag="IXT21_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ4869664.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4627..5868
                     /gene="sstT"
                     /locus_tag="IXT21_002287"
     CDS             4627..5868
                     /gene="sstT"
                     /locus_tag="IXT21_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ4869665.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            6015..6650
                     /locus_tag="IXT21_002288"
     CDS             6015..6650
                     /locus_tag="IXT21_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ4869666.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6841..7584
                     /gene="truA"
                     /locus_tag="IXT21_002289"
     CDS             6841..7584
                     /gene="truA"
                     /locus_tag="IXT21_002289"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ4869667.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7818..8651
                     /gene="msrA"
                     /locus_tag="IXT21_002290"
     CDS             7818..8651
                     /gene="msrA"
                     /locus_tag="IXT21_002290"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4869668.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKKIRKNHF
                     D"
     gene            8732..9725
                     /locus_tag="IXT21_002291"
                     /pseudo
     CDS             8732..9725
                     /locus_tag="IXT21_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            10014..10712
                     /locus_tag="IXT21_002292"
     CDS             10014..10712
                     /locus_tag="IXT21_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ4869669.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10862..11317
                     /locus_tag="IXT21_002293"
                     /pseudo
     CDS             10862..11317
                     /locus_tag="IXT21_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            11398..12524
                     /locus_tag="IXT21_002294"
     CDS             join(11398..11626,11626..12524)
                     /locus_tag="IXT21_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ4869670.1"
                     /translation="MTRYEENFKQMIIELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIILKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            12569..13525
                     /locus_tag="IXT21_002295"
                     /pseudo
     CDS             12569..13525
                     /locus_tag="IXT21_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
BASE COUNT         4595 a         2210 c         2822 g         4073 t
ORIGIN      
        1 atactcgttt acataaaaaa agattattta gatgcgataa tatcatagat aaagaaacat
       61 tcatcataag aaacagtgat cccaaattct gtttcaatat ttttgaaggc cttttttaca
      121 tgaaaaattg tttcatgaga aggtcttgtg tcatcttctg atataatttt aagttcatga
      181 ccgtttatca atgtacgctc aattagacac gctgtatgaa tcaaaagttt taaaagaatg
      241 gaatgagtga attttttgtg gaaagtacat tccaattctt ggataaaatc cattaagctt
      301 ttttggatct tattcccagc taataagtag agatatttgt ccactgcttc aactcccata
      361 tcaaaaaacg atatttcttg tgaatgagtg gctgctgccc ttttttgatt gttgttcttc
      421 agtcgtaaga tgacaaagca tcttatcttt gaataaaaaa atagaactac ctaaggaagc
      481 gtttcggcgc ttcctttttc tatatgatcg tttaatgact gtaggaattc agtataccaa
      541 aagaaaaccg cctttttgaa taaacttgat agtggtcaat tttttatgat ggataagtat
      601 ctatacttag tctcgtaaga atgaaaagga aaagagggag aaagatgaaa ttacaaaagt
      661 ggatccaaga gcatcgaaat catatcaccg ccttatcagc tgtgttgatt atttttggtt
      721 ttatcgggaa atttactgga tttgtcttgt tctataacat tgctctagcc atagcttcta
      781 ttattgcggt catacctatt ataatccatg cgtatcaggc agtaaaagtc aaagttatct
      841 caattgattt actcgttact attgctgtgc ttggtgcctt tatgatcgga gaatacaagg
      901 agtcagctat cgttacattc ttgtttttgt ttggtagtta tctggaacaa aaaacattga
      961 ccaaaacgcg ggaatcaatc aaggctttga ccgagatggc cccatcgact gccagcgttg
     1021 tccaagaaga tggaacgacc gaagaagtgg aaatcgatga cgtggaagaa ggcgatatcg
     1081 tcttagtaaa aactggtgga gctattcctg tggacggacg agtcattgac gggaaagggt
     1141 atgcggatga agccgcagtg acgggtgaat cacgacaagt cgtcaaaatc agtggagata
     1201 acgtattttc tggaacgatt ttagcagatg gctacgtgac gatcgaagca actaaggtcg
     1261 gagatgatac gacctttgcc aaaattatag aattagtcga agaagcacaa gataccaaat
     1321 cacatgcgga aaaattcatt gatcaatttt ctcagtatta tactccaacc gttttactta
     1381 ttgcgttgct tgttggtctg atcagtcgtg attttcgttt agccatcact gttttggttc
     1441 ttggttgtcc tggcgcttta gtcattggtg cgcctgtttc gaatgtagca ggaatcggta
     1501 atggagcgaa acggggcatc ttagtcaaag gcggtgaagc aatggatact ttttccaaaa
     1561 tcgatacttt tgtctttgat aaaaccggta cattgaccaa aggaactact gctgtatcca
     1621 ctatcaagaa ctatacagag gatgaagggg acagcttagc tttagtggct cgattagaaa
     1681 cgttatccga tcatcctctg gggcgcgccg tgatccatta tgctcaggag aaagacattg
     1741 attttcaaca attagcagtc aaagataatc agacgatcaa gggacaagga atcacagctg
     1801 agattgatgg tcatacagtt tgtgcgggca acaccaaatt gattgccgct cacaaacttt
     1861 ctctcacacc aaagcaagta caagatttac atcaattaca acaaagaggc agctcagtca
     1921 ttattgtagc aatcgacaaa aaaatcactc aaatcattgg tgtgtcagat gtgattcgac
     1981 cagaagtcgc tgaacaatta gctatgttga aacaagcagg agcaaaacat ttagtgatgc
     2041 tgacaggtga taatcagatg actgctgatt atgtagctga catgttaggc attgatgaag
     2101 tccatgctga attattgcca gatgaaaagg ttcaatttgt gaaaaaatac caagaacaag
     2161 gattgcacgt tgctttcatt ggagatggaa tcaatgattc tccctctttg gctgcggctg
     2221 atatcgggat tgctatggga tcaggaacgg atgtcgctat cgaaacgagt gacgttgtct
     2281 tgatgcaatc tagttttgga tcgttggttc atgcttaccg tttggctaag aaaactgttt
     2341 tgaatacccg tgaaaatgta gtgattgcca ttggtgtcgt cttattttta ctcatcggct
     2401 tatttgccgg gtttatttac atggctagtg ggatgttcgt tcacgaagct agtatcttga
     2461 tcgttatctt taacgcgatg cgtttattgt attttggaca cgaaagcaaa gaagaaaaat
     2521 cgtaaaactt gataagaatc aattttttta tggaagctgt aatctagaat aagactatca
     2581 agaaaaagga aggaagaact acgatgaaaa aaatgattat gcaattagat acactagctt
     2641 gtccaagctg tatgcaaaaa atagaacatg cggtttctca acagcctggg gtaagcaata
     2701 cgaaagtttt gtttaatgcg agcaaaatca aagcaaactt tgatccagat gaagtccacc
     2761 ctgaacaatt agctgcagtt gtggaaaaat taggttatga agtccaatca acaaaagtaa
     2821 aagaggcgta aatcagtgaa tatagtggaa gaaaaatttg ctcaagaaac aaaacaagca
     2881 gatatcgatc atcataaacc aactgctggc gccatggtgg ggcatgtatt ggcaaatttt
     2941 aaattagaag aaatgaaatt gatccaagca caattgtatg ttttgggtac atcttcgcaa
     3001 gaattaagta cccttttttc agaatatgct gatcaagaag cgagatggtt caataaaatc
     3061 tctcatgagt tagtgattga aaatgaaatc gttccgtcta ctttagaaga agtcatgcgt
     3121 tatggaaaaa tttacgaaca tggtcaaaac aaatatttac cagcctcaac aatgttggaa
     3181 aactttgcaa aagattttga tttccaaaat gtctttatca cacgggcaat caaattagct
     3241 gaaaaagaag aaaaatatgg cttgaaacaa ttgcttgttg aacttttaag tttcaataaa
     3301 gaaatcatct accaagtaca aagactccaa ggaaaaactg tccgacaaga tttggatgag
     3361 gaagacgatg atgactaaaa gtctatgttt cttcaaatac aaatataaag cgtgaggtaa
     3421 attggaaaat cgcgaagatt aaaataaaag aagcacccgc tgatgtatta cagacaacat
     3481 cagcgggtgc ttcttagttt ttaaaaggtt taaaagatgg atgagaaaat acaaaaaatc
     3541 aataaaatca cattgcttat ttgatagaat aaagaagtgg gagtaatcga aaggagtttt
     3601 ttaatttaaa aaaaggattt tagtaacaat tggtctagct tttttgttag ctggttgtac
     3661 caacgggaat gaatcgaaaa aagaatcaac aaaagaaaca gcgaaagcaa caattgcaac
     3721 tactacaaca actagcacag agagcaagcc aactgcagca agtacggaaa cagcagatac
     3781 ctctacttcg tcagagtctt ccaaaacgac tgaaacaact caaacatcta cttcaagttc
     3841 ttctgtagag gcttcatctt cagaaagcag cgaacaagtc gaacaaacac tgtggaatgc
     3901 agacaaatcc gcacaattag aggcatttat ggctacatgg ggccaaacaa tgggacaaca
     3961 atacaaaagc tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagt
     4021 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     4081 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     4141 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     4201 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttta acgagacagc
     4261 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat gggtaagtaa accagtgaga
     4321 gtgagagaaa gaagagactc agtggaaaat attaattgat tgctatgtct aagaattaag
     4381 atgaaggatg aaatttctag tttcgtcctt catcttagtt acgttttact tggataacta
     4441 taataataac gataaatagt tttctctcga cttctttaga gattgaataa accctccaat
     4501 actaaaattg aatgttgcaa gagcatgaat tgccaattgt attattagaa tttttagaaa
     4561 attgcacctt tagtttcttt agataaaatg atacaatgat tgttaaaatt gtgaaagggt
     4621 ggtcatttgt tagagaagtt tttggatatg tcgttagttc aacgtattgt tgttggtatt
     4681 ttagtcggaa tattgtttgg tatctttttc cccacgtgga catttatttc aatattgggg
     4741 acgttgtttg tcagttgctt gaaggcaatc gcacctattc tggttttttt cttgacgatg
     4801 gcgtcaattg ccaagcataa aattgggaat aagacattcg tgaagcctat tttgatctta
     4861 tatttagtgg gaacattgct ttctgcactt gtagcagtca ttgtaagttc aatttttact
     4921 gttcctatta ctttacaaga aacagtatct gaaaaagcac cgcaaagttt ggaaagtgtt
     4981 ttatctacta tgttgacgaa tgtcacacaa aatcctgttc aatctcttat tgatgcgaat
     5041 taccttggcg ttttattctg ggctgtattg ttgggactag cttttcgagc acgttcagaa
     5101 ccgacaaaag agttggtgga tcagatatct atagcgttgt cacaagtcgt acaagtgatt
     5161 attgcatttg ctccgatagg gattttagga ttggtttatc aatcaattgc tacgactgga
     5221 atcgctggat tggcagagta tctgcagctt ttacttgttt tgatcggtac gatgctgttt
     5281 gtcgcattag ttgtgtaccc attcctcacg tttttgttta ttaaagaaaa tccctatcca
     5341 ttgattttct tttgtttgaa agaaagcgca cttcccgctt tctttacccg aagttctgcc
     5401 gcaaatatcc cgataaacat gatgttggct gaaagattga aattgacaaa agagtcttat
     5461 tccatatcga tcccgttagg tgctacgatc aatatgggcg gtgccgcgat taccatttcc
     5521 ttgatgacgt tgactgctgt acattcttta gggattgaag caccatttgt tttgaaagtc
     5581 cttttatgta ttttgtctgc tttagctgct tgtggagctt caggtgttgc gggcggttca
     5641 ctgttattga ttccattagc atgtagtttg tttggtatct ctaatgatgt cgcgatgcaa
     5701 atcgttggta ttggatttat tatcggtgtt atccaggatt caatggaaac tgccttaaat
     5761 tcgtcaagtg atctgttgtt tactgcaata ggagagctag gcgcgcgtaa aagaagtggg
     5821 gaaaaaatca aattgaaaga ttgtttagat ggattaagtg aacgttgaac gttagatatt
     5881 tagtattttt aaaatgattc gagtttttaa tgagttatat agatgttcgt gtaaatagag
     5941 gtttctgtta aatcaagata aagtcgttta taataacaat taaaagcata ggtaattgtt
     6001 attggaggcg tatcatggat tttttacgta aatttggttt tttcttttta ttattaggcg
     6061 tgctgagtga ttttttgact ccgtacgtat taggattgta ttatcctgaa ttagatcaaa
     6121 tgaagagcgt gatcagtgta tttggagagg tttccagtcc tgtaagaaaa gcgtttctta
     6181 tttggtctgt ggtttcaggg atctttttca tcctgtcatt gcctgcattg tatgctgcat
     6241 ttgtaagtac atctcgtgtg ttagctaccc tagttacagc agccatcgcg ttctatggta
     6301 taggtgattg tatctttaca ggtttgttca gtatcaatac ccatgaatct tcgtggaatg
     6361 tctcgacatg ggtccataat atcggatcag gtttaggtta cagtggtttt ttattgtttc
     6421 cagttcttct tttcttgatc tatcgaaaaa atgggcaaat ggacgtaagc cacttatacc
     6481 ttggactgtc tgtgatcagt ttgctttttg cggcgattta tggactggca agaattcctt
     6541 ctataaattc ttggccatta ctgaataaaa taggtttttg ccaaagaatc agttttttct
     6601 ttaattatgt gcccattgcc tggttgagta tcttgcatct caaacagtaa aacttgagcc
     6661 gtcaaattga acagtcagcg gatacttcat ccggtggctg ttttttgttt ttctgaaata
     6721 tatatttcca cagttttctt gtatagcttc tgtcttagag aagcaaggag ctatattttg
     6781 tgctacaatc ttaaaaaggt agagagaaga ccaaatggaa gacagaggag ccactaaaat
     6841 atgagaaaca tcaagttgac aatcgaatat gacggaaaaa gatattcagg atggcaaaga
     6901 cttggagatg acgataaaac cattcaagga aaaatcgaaa aagtattgca tcagatgaca
     6961 aatgaagaga tagaaattat tggatcagga agaacagatg ctggcaccca tgcgcgagga
     7021 caagtagcaa attttaaaac aaacacagaa attggactgg cagaaatgat cgactttatg
     7081 aatcgttacc ttccgcggga tattgtcatc aaaagaatcg aagaaatgcc tgagcgcttc
     7141 cattcaagat ataatgcggt gggcaaaaaa tacagctatt atgtttggaa caacgtgatt
     7201 ccttcagcat ttgagcgaaa ccacagcttt tatttccctc aagagttgga tatggacaag
     7261 ctgaatgcag cttgcgaaaa attagtgggt acacatgatt tcattggttt ttctgcattg
     7321 aaaaaatcaa aaaaatccac agtgcgtacg attgatgaat tatctatcga acgaaaggga
     7381 gaaatgcttc attttacttt tgtcggagac ggattcttat ataaaatggt gcggattatc
     7441 atggggacat tactagaaat cggcacaggt gccatgccga tagaaacaat cgatgaaata
     7501 tttgaaagca aagtgcggca acatgcagga gaaaccgtcc ctgcacaagg tctgtttttg
     7561 gatgaagttt attatcgctc ataaaaaaga atcatcttta ctcgacttct gagaattgga
     7621 gaacatccct tttttcagag gtcgtttttt agttaaacca aaccatagag tatgcttttt
     7681 aagataaaga tcatcttata tcatctgaag ctgcttatct tctttgaata aatacgattc
     7741 aatcgattat ttcctcactt tatgataaat tacagctaaa gtcactagct tgtcttcttc
     7801 ggaaaggagg agaatctatg gatgaggaac aaatattcat tcaaacactt tataatctga
     7861 ttttgaatcc gaatacacga gattgggaac gaaaagtatt gattcagaca aagaatgaca
     7921 caagagaaaa tatatccgtt aaagaacagt tatcaaaact agaagtaaca ctacgtccat
     7981 tagctatccg aatgaatctg acgcctgatg taatggactt ttatttattg attacggaag
     8041 gatttgataa ggagcaaaaa tatgactttt ccaagcatgc catgcaagat gctgactatc
     8101 aagaacgtgc agtatttgca gggggatgtt tctggtgtat ggtcgagccg tttgaatcga
     8161 aaaaagggat cctatctgtt ttgtcaggat acacgggcgg acatgttgaa aaaccgaatt
     8221 atgatcaagt aagtggtggt tacacaggtc atgtggaagc tgtggagatc atttatgaca
     8281 caagagaaat aagttatagc gagttgctga caatctattg gcaaattact gatcccacag
     8341 atacatttgg acaatttcaa gatagaggga agcaatatcg tccagtgatt ttttatcaag
     8401 acgaacgaca aaaagaatta gcggaacaat caaaacagaa actagacagt tcaggaactt
     8461 tccaccaacc aatcgtcaca aaaatcgaac cagccggtac gttttggcca gcagaaaact
     8521 atcatcagca attttacaaa aaacaaccga aacgctataa aaaaatccag caagcacgta
     8581 atcaatttct gatctatcaa cgagtaaaaa acaaatggca aaaaaaaata cgaaaaaatc
     8641 attttgatta actccttttc tttaaaatgt agtaaactaa aaatagaaaa caagaaagcg
     8701 ttttaaaaag atttgatgag gaggagaaaa attgtatatt gcagatagtg cacgttatga
     8761 aaaaatggaa tatcgtaaat ccggtcattc aggtttaaaa ttaccgattt tatctctcct
     8821 ctcggattat ggcaaaattt tggtgattat gatcctatcc ataatcaaag agagatttta
     8881 agaggcgcat tcgacatggg aattacccat ttcgaccttg cgaataatta tggaggccct
     8941 gctggagctg cagagaaaaa ctttgggcgt atttttagag aagattttca agcctaccgt
     9001 gatgaactga ttatttctag caaagcaggt tatcatatgt gggaagggcc atatggggaa
     9061 tggggctctc gtaaatccat catatcaagt tgtgaccaaa gtttccaaag aatggggcta
     9121 gattatgttg acatcttcta tcatcatcgc ccagaccctg atacaccttt ggaagaaaca
     9181 gcagaagcgt tgatgcaact tgtaagacaa ggaaaagcat tatatattgg tatatccaac
     9241 tataatggag aagatacaaa aaaatgactg aaattttaaa aagaaaggaa gcaccgttca
     9301 ttattcatca gatgcgctac aatatgtttt ctcgtgcact actagaagac gatctatctc
     9361 cagtattaga agaagaaggg ctaggggcaa tcacgttcag tccgcttgct caagggttgc
     9421 tgacaaatcg ttatctacat ggtattcctg aagattctag agcacatcgt aaggaaatac
     9481 cattcctttc agaagaacaa gttggatcaa ctctggaaaa aatcaaagca ctgcaaacaa
     9541 tcgccgtatc acgaggacaa tcattagcgc aaatagccct tgcatggaat ttacgacaaa
     9601 aatctgttac aagtgtactg atcggagcaa gtcggttaag tcagttacaa gaaagtgttc
     9661 gaatgatgga taatttagat ttctcaccag aagaagaatt gagaatcgac caaattctag
     9721 aatagaaacg atgtaatcaa actttttatc catatattaa aaaaaacaaa agatgacatc
     9781 aagaatcgaa tgaagatcgt ctgttattga acatcatatt ttttgtcgat caaaataaaa
     9841 atgatcgaag gctaggagtg aattttatcc tagtcttttt tatggtaaat atcttcttcg
     9901 tttgtatgtc tatgaacgga ttaatcgatt ttttatacaa aaaatcatca gatgattcaa
     9961 acgtgttata ataattacga aaaaatacat cagatgaaaa ggaggtatgt ggtttggaag
    10021 aaaataaaac cctagtagaa gtaactactg acaagatcct tagatattta aaggaaatgc
    10081 agttgcaagt aggagataaa ctgccgaatg aatatgaatt gtccgattat ttagaggtgg
    10141 ggcgaagcac ggtgcgagaa gctgtgcggg ctctagcttc caggaatatc cttgaagttc
    10201 ggcaaggagc ggggacattt gtcagtgata agcgagggat tgccaatgac ccactcggtt
    10261 tttctttgat tgaagatcag gatcggatga tacgagactt atttgaactg agatatttat
    10321 tggagcctaa gatggcaggt ttggcagcgg aatatgcaac gaagaatcaa atcgtccaaa
    10381 tgaaaaaatt aacgcaaaag attgaaactt cttttcgaga aggcacacaa gaacatgttc
    10441 aattagacat cgattttcat acgatgattg cagaagctag cggaaacata gccttgctgc
    10501 atattatccc aatcattaat gaatcgattg ctttgtttaa taaaaattat gatatccagc
    10561 atctgaaaat cgagacgatc gatattcata cagagatcgt tcaggccatc gaaaggcgga
    10621 acgtacttgc tgcgtttgac gcaatgactg tccatatggc aataaatcgg agagaattac
    10681 agaaaataat aggagaaaaa gaaactaatt gacagttcat ctgatgtatt ttatgataaa
    10741 tacatcagat gattttttgt tttatatttg gtaaaaaaga ggagtaaata gtttctgttc
    10801 accaaaatgt aagcgctgac taatagacaa aaagagatga cgtcaaggag aagaagcagg
    10861 aatgagggga tctggaatga gttttatgga taaattagcc aaagttttag ggaacgtggc
    10921 aacgagaatc aataatttaa ggtatatcat ggtgataaaa aacgcatttg ctgcattgat
    10981 tccggtaatt attacgggtg cttttggcac attgttttca gcaatggtct ttgactcgga
    11041 aaatggctta gcacagataa gttacttgcg ttttttagaa gaattaaagc ctatcgcatc
    11101 tgccatcagc tatgtcacac taagtttctt gaccatctat gctgtttttc taatagggat
    11161 cgaattagcc aagctgaata aagtggatgg tgtattccct ggtatcatcg cagtgatgtc
    11221 ttatttatct gttaatccaa ctgtttatga attagtgaat gaaaatcaga ctgttatagt
    11281 agaaaacgta ttggctaaac aatatacaga cacgaaagtg gtgttagtat caaatcttag
    11341 acagcttttc gtagagacaa gaataaatta aacttatcta cgagaggatg aatttttatg
    11401 acccgatatg aagaaaattt taaacaaatg attattgaac tgaatcaaac tggacgttct
    11461 gttcgagggt tagcgaaaga atatggctta tctgaagcaa cgatttacaa atggaagaat
    11521 ttatatttac ctgatcagtc cacaggactg actggaaaag aagtagctga actgagaaaa
    11581 gaaaatgctc gtttaaatga ggaacttgaa atcttaaaaa aagccgcagc catattctct
    11641 cggaaaacct aaattcgctt gttcaattta ttgaaaaatg gtgtaaggat tacactgttt
    11701 ctttgttgtg tcggttatta gaaatcccta gaagtgtcta ttatttttat aagaataaac
    11761 cgttgacagc tacagaaatc agaaataata agttgaaaaa gaagatttca acaatttttt
    11821 ttacgaataa acaacgctat ggtgccacaa agatccatca agttttatta aaagaaggga
    11881 tttcagtatc tcttaaacat gtccaaaagc taatgaaaca attaaattta aggtcgattg
    11941 tagttaaaaa atatagacct caaaggtcta ataaaccgat cattttaaaa gagaatctct
    12001 taaatcagga cttttctact gaaacgatct gtgagaaatg ggcagctgac attacgtaca
    12061 ttcctactaa gaaaaatggt tggtgttact tatcttcaat catggattta cacacgaaaa
    12121 aaattattag ttatacattt tcaaaacgaa tgactgtgga ttgtgtcatt caaacattga
    12181 ataaagcaaa aatacattat cacattccag aaggaatgat tctacacact gacttgggca
    12241 gccaatacac agcaagagaa gtggaacaat ggcttaaaac caacaaaata aggcattctt
    12301 atagtagaaa aggaacacct tatgataatg ctggaatcga atctttccat gcctcattga
    12361 aaaaagaaga agtctacacg actagctact cagattttga agaagcaaat cgagcgctat
    12421 ttagctacat tgaaggattt tataaccgaa atcgaatcca tagctcgatt cactatctaa
    12481 ccccacagga atttgaagaa ttagcaaaag aaaaaatggc ttaaccgtct ctactaaaat
    12541 gtgtccaaga tattgactca aatccattga tcgttacggt gatagggtaa aaaactggtt
    12601 tacattcaac gaaccaatcg ttccccaaac gcgttgctat ttggatgctg ttcgctggcc
    12661 gcatgaacaa aacacaaaaa agtggatgat gtggaattat cataaagttt tagcaacggc
    12721 taaagcagta gagatttacc gcagcggaaa ttatggtgga aggattggct gtattttaaa
    12781 tcctgaaatg gtctatgctc gatctaattc tccagaagac caaaaagctg cttatatgta
    12841 tgatttgttt tataatcgag tattttttga tccgatggta aaagggtact acacagaaga
    12901 gttcctggag ttgtgtcaaa aacaatcgat ttatttcgat cctgcaacag aggatttaga
    12961 aacgattcag catcatacag tagatttcct tggaatcaat caatattatc ctaaacgcgt
    13021 tcgcgctcct cgttatcaat ggaatgaaga gacacctttc catccagaac gatattatga
    13081 agtctttgat ttacctggga agaaaatgaa taattccagg ggatgggaaa tctatccgaa
    13141 aatcatgtat gatatagcca tgtatctaaa agaaaattat cataatatcc cttggctgat
    13201 aacagaaaac ggtatgggaa gagaacatga agaacggtat atggatgaga aaggaatcgt
    13261 gcaagatgat taccgtatcg aatttatcgg acagcatttg cgttatttga tcaaagcaac
    13321 agaagaaggc gcaaattgcg aaggctatat gttatgggca ttcactgact gtgtgtcacc
    13381 aatgaatgca tttaagaatc gatatggatt agttcgaatc gaattgaacg atgaacgaag
    13441 cagaacattg aaaaaatctg cttcgtggta tcagactttg attgataaga gagaattgga
    13501 tttgcctgac gaaatctata aataaataga agttttttgt agatgttcat tgtatttact
    13561 ggttatttga aataaaagag gttgtgaaaa agctgtcctg catcaagtaa taagaatagc
    13621 aaagtaaaaa caacatctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
    13681 ttttgaatac cgtttattcg
//
LOCUS       DACGLH010000077        13691 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.082, whole genome shotgun sequence.
ACCESSION   DACGLH010000077 DACGLH010000000
VERSION     DACGLH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.082"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(623..695)
                     /locus_tag="IXT21_002296"
     tRNA            complement(623..695)
                     /locus_tag="IXT21_002296"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Lys,seq:ctt)
     gene            1076..1645
                     /locus_tag="IXT21_002297"
     CDS             1076..1645
                     /locus_tag="IXT21_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ4869671.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1632..2492
                     /locus_tag="IXT21_002298"
     CDS             1632..2492
                     /locus_tag="IXT21_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4869672.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2682..3386
                     /locus_tag="IXT21_002299"
     CDS             2682..3386
                     /locus_tag="IXT21_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4869673.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3391..5226
                     /gene="walK"
                     /locus_tag="IXT21_002300"
     CDS             3391..5226
                     /gene="walK"
                     /locus_tag="IXT21_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ4869674.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5223..6539
                     /locus_tag="IXT21_002301"
     CDS             5223..6539
                     /locus_tag="IXT21_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869675.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKVKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6540..7409
                     /locus_tag="IXT21_002302"
     CDS             6540..7409
                     /locus_tag="IXT21_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869676.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7464..8273
                     /locus_tag="IXT21_002303"
     CDS             7464..8273
                     /locus_tag="IXT21_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4869677.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8376..9005)
                     /locus_tag="IXT21_002304"
     CDS             complement(8376..9005)
                     /locus_tag="IXT21_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ4869678.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9175..10467
                     /locus_tag="IXT21_002305"
     CDS             9175..10467
                     /locus_tag="IXT21_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4869679.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10543..11205)
                     /locus_tag="IXT21_002306"
     CDS             complement(10543..11205)
                     /locus_tag="IXT21_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ4869680.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFGVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11430..11714
                     /locus_tag="IXT21_002307"
     CDS             11430..11714
                     /locus_tag="IXT21_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ4869681.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11765..13390
                     /gene="groL"
                     /locus_tag="IXT21_002308"
     CDS             11765..13390
                     /gene="groL"
                     /locus_tag="IXT21_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ4869682.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4581 a         2345 c         2918 g         3847 t
ORIGIN      
        1 tacttcagta tcccagactt tatataaatc tttgacaaat ttttctgatg gatcgtattt
       61 aaaccaaagc agatgttcct ctagctcgat tgtcatgttt ttcacctcaa cgaaagtata
      121 cgaactaatg tttgttttgt aaaggataaa aatagggtag caaccggtac ccaaactagt
      181 acccatttat caaaaacgac gaaatttatg taaatagaaa aataagaata aacattgatg
      241 tatcaacatt tatatatctt tagaacaaat gaaaagggtg gttctcataa gaaaaacatg
      301 ttcgcacttc taggcaaacc aggatttgaa gaccttgcta aagaattgaa cgaacgcctg
      361 taaaataagg atttgtcgct agatttaaaa aatcagtgat agaaacatgt ttgctttaaa
      421 atgaaactag tgatagtttt agtgatagtg aacttaaaaa aagcactcaa tttgagtgct
      481 tttttattgt tattaaaaat taaaaaacta aaatacatac ccgaatttca tacccgaaat
      541 ataaaaaatg tcatcacgta catctttttc gaaactaccg aagtagccaa agatgtactg
      601 atgacgtggg tttggcaccc aatgggccgt gaggggctcg aacccgcgac ccgctgatta
      661 agagtcagct gctctaccaa ctgagctaac ggcccgtaca aaaagtactt aataatagta
      721 gcactaagaa ttttaaaatg caagactaca tttaaagaaa atcagaaaaa tctttacttc
      781 cttataggaa atcattcata gattattcac atttgctcgc tattatataa gcataccaac
      841 aaacaaagtt cttgcaagag aacaaaccct aacacttttt tcatttttta ctcctttccc
      901 gccgtctccc cggcgggtct cctgttctta acgataaaag acaagaaggc ttagccttct
      961 tgttttttgt tttcatggaa tcccgctaat caaatcacct agttaaagca actagattga
     1021 atgtttatca taagtctgat atcatgttta tgaaatgaaa aaaggagcgg ttatgatgga
     1081 aaaaatgcgt cagcaattaa tggagtgggg gaaggaacta gaaacattcc gtctgcctca
     1141 ctgggaagaa ctccctgact tagaacttta tatggaccaa gtcagaacgt tagttgatcg
     1201 ttatctttca cctgtgatac aaggagaaaa gcatccattg cttacctctt ctatggtaaa
     1261 caattacgtt aaactaggtt tgataccagc accagttaaa aaaagatata acaaagaaca
     1321 cgtagcattt ttacttgcaa tcactacgtt gaaacaagta ttgactattc ctgaaatcaa
     1381 agaagggata ttgttccaag gaaaaacagt aggaattcgt gaagcctata atctattttg
     1441 cgatgaacaa gaagcagctg tgtggatggt cagccaatta gcacaaggta aatctcatcc
     1501 gcaaaaattc gaccaggcaa cccctgtaga atatattgct gtaagagcgg caactttatc
     1561 gtttgcaatg aaacttcttg cggaaaaaac gattgttcta gaaaccgaat atttaaaaga
     1621 ggagaaaaca aatgaaaaac aataaaatcg caattttagt agattcaggt acggatgtgc
     1681 cagaagaatt gataaaaaaa tacgagatgt atatgatccc attgaaaatt atttataaag
     1741 atcaggtata tacagataaa attgacatta cctctgaaga gatctatcaa cgtttgccag
     1801 aggaaatccc tggtacatct ttgccggatg gtgagtcgat cacagaaatc tttgagcaaa
     1861 tcaaaaaaga cggttatgaa aaggtacttg cggtaacaat ttccagtgga ttgagcggga
     1921 catataatgt ggttcgttta attgcacaac agcaagaaga attggaagca tatgtactag
     1981 atacgaaaaa tattggtatt ggtgcaggtt tttctgcgat ccaagcagca aaatggctgg
     2041 aagaaggagt agaatgggat caactgatca gtaatctgaa tgagttagtc gaaagaacaa
     2101 aagttttctt taatgttgcg acattggaat atcttcaaaa aggtggacgg atcggtttag
     2161 ttgcttctat tgtaggaacc gctttaaagc ttaacccaat catctcatgt aatgaagaag
     2221 ggatttatta cacagtagga aaagctaggg gacgtaaaaa aagtttggat aaaacggttg
     2281 cttacgtcaa agaaaaaatc ggtaatgcaa aggtcttcaa cttagctgtt gcccatgggg
     2341 atgcgaaaga agaagcaatt gaaatggaag cgagacttaa aagagagttc ccacaagcaa
     2401 accaaatcta ctttggccag atttctccgg cattagtagt ccacactggt ccgggattat
     2461 tgggtgtcgg tgtacaagtt ttagaaggat aaatcaaaaa aaggaaacac tgaggtgaaa
     2521 atcagtgttt tcctttttta ttgaagttag aacaaaaaat aataaagtat tttgcacttt
     2581 cattttaatt ttctcttttg ttttataggc aaagatgaaa caaaagcaga aatacggtat
     2641 aatgagaagg tatgacaata atatataaga ggtggctctt tatgaaaaaa gttttagttg
     2701 tcgatgatga gaagccgatt tcggatatcg tcaaattcaa tttagccaaa gaaggctatg
     2761 atgtttatac tgcatatgat ggggaagaag ccttggaaaa agttgctgag gtagaacctg
     2821 atttgatttt acttgatttg atgctaccta aaatggacgg cttagaagtt gctcgtgagg
     2881 tacgcaaaac ttatgatatg ccgatcatta tggtaacagc caaagattct gaaattgata
     2941 aagtcctagg cttagagtta ggcgccgatg attacgtgac caagccattt tccaaccgag
     3001 aattagttgc acgtgtcaaa gcaaatcttc gtcgcggtgc cacggctgcg aaggaaccag
     3061 aagaggcagc acctgcggaa ttgacgattg gtgatttgac gatccatcca gaagcttaca
     3121 tggtgacgaa acgaggagaa acaatcgagt taacacatcg tgaattcgaa ctactctttt
     3181 acttggcgaa gcaccttgga caagtgatga caagagaaca tttattacaa acggtttggg
     3241 gttatgatta ttttggagat gtacgaacag tggatgtaac tgtacgtcgt ttgcgtgaaa
     3301 agatcgaaga taatccaagc catccaaatt atttagtgac acgtcgtggt gtcggttact
     3361 atttgcgtaa cccagaacag gagtaattgg atgaaaagaa aagtccgctt ttttcgatca
     3421 gtcaatttta agatcgctat tacctttatt ctgattttat tgatctcgat cgaaattatt
     3481 ggtgcctact ttatacgtgg tttagaacgt tccacgatca atacatttat taaagatatg
     3541 aatcaaacgg tggaatcatt agcaacaaca atcagcccgg aattgaatcg aaaagataat
     3601 gcagatgatg aagaggtaaa tgcgaatatc aaaagattta ttgaaaacag tgcgactagc
     3661 gatatcatcg aaatacgggt cgttgacgaa aaagggatca tccgaggaac aaccgatgtc
     3721 aatgaacaaa gcgctgtggg aaagaaaaat gattatgttg atatcaatga tttcacgact
     3781 aaacgttatg ttgctttaga taacgacgat aaacgggtga atatcaatgt tcaaccgatc
     3841 ctgtccccga ccggcgatgc cgttatcggg gctctttacg tcaaaagtaa tatcgaacaa
     3901 aaatattcgg aaatcaataa cacagcagtc attttcttta ctgcctcact aattgcggca
     3961 tttatttcaa tgatcgtatc tgttttagtt gctcgttcga ttacacagcc gatcggtgaa
     4021 atgagagaac aagccattcg aatcgctaga ggagactaca gtcggaaagt caaagtgcat
     4081 ggacaagatg aacttggaca gctggctgat accttcaatc agttagctga acgtattgaa
     4141 gaagctcaag atacaatgga agctgaacgc aatcgtttgg atagcgtgct atcccatatg
     4201 acagacggcg tcattgcgac agatcgcagg ggtaaagtca ttacgatcaa tgatatggct
     4261 gtttccttgc tagatatcaa aaacgaggaa gcaatcggac aatcgatttt gacactttta
     4321 gatatcgaag aagaatatac gttgcggaaa ttattagaag atccaaacga aatggtactt
     4381 gaccgttcaa ccagtacatt ggaaagtgat cagatgatct tgcgagtaga cttttctatg
     4441 atccgtcgcg aatcaggttt tatcagcgga ttagtagctg tcttgcatga tgtgacagaa
     4501 caagaaaaaa cagaacggga acgtcgtgaa ttcgtctcta atgtttccca tgaacttcgt
     4561 acaccgttga caagtatgcg tagttatatc gaagcattga gcgaaggtgc gtggaaagac
     4621 gaggaagtag caccaaattt tttgaaggta acgctagatg aaacagaccg aatgattcga
     4681 atgatcaatg acctcttaaa tctatctcgc atggatacag gtaataccca gcttcaatta
     4741 gaatatgtca atttcaacga gttagtgaat tttgttcttg accggtttga tatgatggtg
     4801 ggaaatcagg aaaaaaatta taagatccgt cgtgaattta cgcaaagaga tctttgggtc
     4861 gaagtagata cagataaaat catccaagta gtcgataata tcatgaataa cgcgatcaag
     4921 tattcaccag atgggggaac gattacttgt cgcttattag aaacgcataa taacgtggtt
     4981 ttaagtatca ccgatcaagg attggggatt cctaaaaaag atctgaaccg agtgttcgag
     5041 cggttctatc gagtagataa agcacgtgca agagcacaag gtgggacagg acttggatta
     5101 gctatttcta gagaagtgat taaggcgcat cgaggcgcta tttgggcaga gagcaaggaa
     5161 ggaaaagggt cgacttttta tatctcgctg ccatatgaac cttatgagga ggaatggtgg
     5221 gaatgagaat ctctgaaaaa gtcgttcgta taggactcat tttgatggtt gcgttaagca
     5281 tatacttctc ttatgcaatc tggctgagcc cagcaggaaa aagttcaatc aacgtagatg
     5341 agacaaacac tcaaatgatc gagtctcaaa gcaatcgaaa aacttccgaa gtctttcttc
     5401 cgcttcatgt tacatggcac cattcgggaa tgatccaaga aaccaacagt gaaaatctcg
     5461 tgtaccgatt gcaattgatc atcgataaag caagatttgg aaagctttct gaagtagttt
     5521 caggagacaa atcacagttt gaacaggtga aaaatctgaa ctctggtttt gagttggcct
     5581 ataatgcgcc ttttttgcta agagaataca aggaagcatt tgatttagat ttagatgtgt
     5641 cgaacttggc aaaagaaagc gaaaatatgt attttactag aatacaattc gatcaaggaa
     5701 ataaaaaaat ccgatttttg aattattcga accatctcgt atatgaagca aatatttcgt
     5761 tgaattggac agatatcaat aaagaattaa cagcaaaaga cacaaaatgg acagaaatga
     5821 caacagaacc atcgacgatc gacacgcaat atgctacgaa acattcgatc aaactgaaaa
     5881 agtatagtta tatcctgtca cagcaaccct atacactttt tagaaatgct tttttccaga
     5941 aaccagatag cgtgaaaaat aatgatgaaa gcaatgaatt gattttttac ggtgggaatg
     6001 aaacaatgtc tatccattcg gaaacgcaag tagttgattt tcgtggagag ctgccagaga
     6061 aaaaagaaaa tgctagtgta ttttctgaaa gtttccccta tattagcaaa ttaggcagtt
     6121 ctttagggaa cctcagatat tttgaccgca gtgacaatac gatcgattat cggatattcg
     6181 tggaaggctt cccggttttt gggacagaca gcagaggaca gattggtgtg gaagttggcg
     6241 gtgaaacgaa tggttctgtg caagtgaata tccaaaccag tttgaatacg attcaagtgc
     6301 cgataccttc agacgaagaa gtagaacttg caagtacgaa tgaaatcatc cagcagttaa
     6361 aagttaaggg agcagatagc aacaagattg gttcagtgat cgttggctat acatggcaaa
     6421 atgtcaaaga aacgaatcgt gtcgttgatc tcttacctga atggtatgtg aaatataaag
     6481 ataactggta ttcagctaac gaccttctgg aaaaattacc tggattggag gcgaattaat
     6541 tggatttcaa aaaaatcgaa tggatatttt tcgtcgcttt tttagggttg aatgtgtttt
     6601 tattcagcag ttatcatgac gcggtatacc aagaaaacaa tgtcattcgc tctaatcaga
     6661 ctgaatcaat ccaacaacgt ctggctagcg acgatatcac ttacacgggg aagatttcgt
     6721 ctgagaacaa gcaaggctat tatcttagcg cggagcagac caatcttagc acttcattag
     6781 cgaattatcg caaggctggt aaaagcggct tgctggcaag tggtgtgttt ttagacggtg
     6841 atgttttgac gaaatcttta tccagttcag atgctgaaaa gaacgtgatc gatccagata
     6901 aaattagtag ttccttggaa gattttttat ctaacaaaga caatatttta ttcggtaatg
     6961 attatgtgta cacgaaaaac ttttctcatt tagaagaaga accactagaa atccaagcaa
     7021 gccaagagta tgaaggcatc ccattcaatg atgatacagc aaaaatcgtt ctttctttag
     7081 aaaaaaacga taatgattat agcatcacga aatacatgca gacgcattta tctgatatcg
     7141 agcagctgcg cgaaaagacg gaactgtata cggaagagga agcaatcaat acattgtaca
     7201 tcaataacaa aataccaaga ggatcaaaaa ttttatggcg tcagctagct tattcctgta
     7261 ttctgaaagt ccgggaaaaa aatgtgtatg ttcctgtttg gcatgtagcg atcgaaacaa
     7321 gcgataaagt ggtccaaatt gagagtgtca acgctttcag caatacgatc gtgacaaaca
     7381 atactgtacc aaaagtggaa gatcaataaa aaagcgagta taatgaaaaa agtttacaaa
     7441 gtaagaaggg aagtaggagt caaatggctg aagaaaaacc agcttttaaa atcagtatcc
     7501 tagcaagtgg aagttcgggt aattcacttt atatcgaaag tgaaaaaaaa cggcttttgg
     7561 tcgatgcagg acttagcggt aaaaaaatca cttccttgat ggctcaaatt gatcggacac
     7621 cggaccaatt agatggaata cttgtgacgc atgaacatcg agatcatata catggcgtgg
     7681 gtgtcttggc tcgtaagtat catttggatg tttttgccaa tcagcagact tgggaagcga
     7741 tggaaccact gattggaaat gtaccagtgg aacaaaaaca tctatttgag atggggaaag
     7801 tcagaacatt cggcgatatc gatattgaga gtttcggcgt ctcccatgac gcagcggctc
     7861 cgcaatttta tcgtttccat aaagaaaatc ggtcattcgt catgttgacg gacaccggat
     7921 attgcagtga ttatatgcga ggtgtcattg aaaatgccga tggttatttg atggaaagta
     7981 atcatgatct tgagatgtta cgcatgggac cttatccgtg gaatttaaaa cagcgtattt
     8041 taggagatag aggacatctt tccaacgaag acggtgcact tgtcatgact gatgtgatcg
     8101 gtaatcggac caaacgaatc tatcttggcc atcttagcaa ggaaaacaat atgaaagaac
     8161 ttgctcattt aacaatggaa aatgttttga aagaaaaaga ttttggcgtt ggacatgatt
     8221 ttgaaatcta tgatacagat ccagatgaag caactgagct attttcgatt taaacagttc
     8281 aatcaataaa aatataaaaa gcagaagata aaatcaatca gggttggttt tatcttctat
     8341 ttttattttt ttagcatatt aaaaaaataa ccgtattatc gactgactac actgatgtgc
     8401 tgcttttcat gtttctcatc cagtatctca tctactaatg caatcgcgta atctgccatg
     8461 ctgatcgtac tgtcgccagc agcgtttctt tgtaaataat catcacttaa caaataagta
     8521 cctgtacgtt tgccatgtgg atcaaataaa gaagctggac tgatataagt ccaatgtaga
     8581 tttttagatt gtttcaattt taacaatcct tcgtacatat tgaaagctgt tggatagaag
     8641 ggtgcatttt taggtacttg gtcaatcata cgagtcgtct tttctgtatc taagaataac
     8701 gaagaggcac ccccaacgac aattaagcgt gtatcggttc cttcaagcat ctcaatcaaa
     8761 tgggacaaag aagtttgatg catttcttct tttcctttgg gagcgttgaa agcatcaacg
     8821 acaacatcaa aagatgcaag atcagtagtt gtcaactcaa ataaatcctt ttgtataaac
     8881 ggaacatctg atggcaattt ttctggatga cgcacgattg ccgtcacgtc taaatctctg
     8941 gacaatgctt cgtctaaaat aaatgaacct gcatggccag tagctccaat gagtgcaatt
     9001 ttcataaaaa acctcctttt ttattcctgt ccattgtaag cagttgctga atattcgtca
     9061 aagaatccga tttttgaaaa gctcagtgcg cattcctttc atggttcgtg atcattgggt
     9121 atagttaaat taagaaaact gattgataaa aatagaagtt gaaggagata agaaatgaaa
     9181 gagtggttag atcgtgcctt tttaccaatg ataacatctg ttgtgaatct tagaccctta
     9241 actgctttgc gtaatggaat gacttacacg atcccaataa ttattgttgg ctcgctgttt
     9301 ttcttatttg ctcattttcc tgtggattac atcgctcagt ggatagacaa aacaggattt
     9361 tctatgtatt ttacacaagt ataccaggct acatttgatg tgatagcctt ttggatcgtt
     9421 tttgccattg catacgctta tgcaaaagaa gaagacatcg atcgcttacc agtcggcata
     9481 acagccttgt ctgcttatct actgcttatt cagccactaa atactcagat agatactgtc
     9541 tggacaggtg ctgaagggat actaggagca gttacaactg ggctcatcat cggatggggc
     9601 tatgcacgtt tagtaagact tgcgaaaggg tggaagcaaa gagagaagat acctgaaaat
     9661 gtttggcagt cttttacttc attgattcca atcattgtta tagtcacttt tacgcttatc
     9721 ggttcagcgg tttttcaaca gttgttcaga atgactgttg ttcaaggagt atggacatat
     9781 gtccagcttc ccattcaatc gttcatagac acgttgtttg gtgtgctttt attaggtttt
     9841 tttgttccgt ttttatggtt atttggtctt catggatctg cgatggtcaa tgggttggtt
     9901 agtcccgtct tgcaagcgaa ttcattagcg aatgcagaaa tcttagcctc aggacaagag
     9961 ttgacggtgg caaatggcgg acatatcgtt actcagcaat ttctcgatca gttcatgacg
    10021 gttacgggtg ctggattgac gttaggggct gtatttttta tgacggtttt tgcgaaatcg
    10081 aagaaataca gagaattagg aaatcttgct cttttaccag cctttttcaa catcaatgaa
    10141 tcaattattt tttccacacc gatcgtgatg aacccgatga tggctgttcc ttttattttc
    10201 gcaccggtcg tttcaggatt gattacctat tccgctttat attttggatt cgttccttta
    10261 tttacagcag tccaagtccc ttggacaaca ccgccgatcc tctcgggttt cttagtaggc
    10321 ggagtgcctg cagcgatttt acaaggtatc gtgttgtcaa ttagtttttt catttatttc
    10381 ccatttttga aaaaaataga ttctgcaaac tgcaaaaagg aaagacagac aaaaaatgat
    10441 ggagcagcag aaaagatgaa cgactaaaaa acaaaaatac tcctgtcgct ttagattggg
    10501 aaaatcacca atctaggatg acaggagtat ttatagtcat gtttatacta gccctttttg
    10561 gacagctaat tgaacaagaa caactaatgt gttcattcct acgtgtgtga tcatcgatgt
    10621 ccagatccga ccagttgctt tgtactctag gctgaagaag aaacctagga aaaagtagat
    10681 tagcagatga ccatcattat gagcaaacgc aaacagcaaa gagctgatca ctgctccgac
    10741 cccaaaattt gaatatcgac tgatgatccc cagtatactg cggcggaata cgaactcttc
    10801 catgatgggt ccagcaacag ttgtcgccaa aataaaagcg ggtgcttcca tgatcatctg
    10861 gatgatattt tgtgtattct cggaaggggt tgctttgcca aacaatacag attctaacat
    10921 gacagcgatg ctttgcagaa tcaatgcgac aaagattcca accaatccat aaataacaat
    10981 ctttggagca gaagcttttt ttaccgctga ttcgatcgct aagggttcac gttgcttaaa
    11041 ataaaaaata gctaggacaa cggcgcctag tatatacatg actgcagttg ctgtgatcaa
    11101 ttcagacgta gattttacaa gtcctatcga agctaggatc aagggtgaga taaaaattag
    11161 accatagcaa aaaatgctga ggaagctata tttttttaaa gacatgtaat tcctcctttt
    11221 aagggataac aagcatgcaa ggcaagctta tatcgattga tttcaattat atcataattt
    11281 caataagcag ctgtcagaat aaaatttctt ttctttgatt taaaagcttg caaaaaagag
    11341 ggagaatgag tattattaaa aatgtaggtt agcactcgaa ggttaagagt gctaacagat
    11401 taaaaaaaga gttatcggag ggatttatcg tgttaaaacc attaggtgat cgcgtcatta
    11461 tcgaagtcgc aaaagaagaa gaaaaaactg tcggaggaat cgttttagct tctgctgcta
    11521 aagagaaacc ccaaactggg acagttgttg cagtcggtga aggccgtttg ttagaaaacg
    11581 gtgaaaaagt tcctgccgct gtaaaagcag gcgaccaagt tatgttcgaa aaatacgccg
    11641 gtacagaagt aaaatacgaa ggaaaagaat atctgatcgt cgcaggaaaa gatatcatgg
    11701 cgattgttga gtaaatttgg aagataaaaa aataatcaaa aataatcaat gaggtgaatc
    11761 aatcatggca aaagaattga aatttgcaga agacgcacgc gcagctatgt tacgcggggt
    11821 agataaatta gcagatacag tgaaagtaac attaggacct aaaggccgaa atgtcgtact
    11881 agaaaaatct tacggctctc cattgatcac aaatgatggt gtgacgatcg caaaagaaat
    11941 cgaattagaa gatcattttg aaaacatggg tgcaaaactt gtatcagaag tagcatcaaa
    12001 aacaaatgat attgcaggag acggtacaac gacagcaact gttctgacac aagcaatcgt
    12061 tcgcgaaggt ttaaaaaacg taactgcagg tgctaatcct ctaggcattc gtcgcgggat
    12121 cgaattggca acaaaagcag cagtcgaaga attgcataat atctcaactg ttgttgattc
    12181 aaaagaagcc attgcacaag tagcagctgt ttcttcgggt tctgataaag ttggacattt
    12241 gattgctgat gcaatggaaa aagttggaaa cgacggtgtc atcacaatcg aagaatcaaa
    12301 agggatcgaa acagaactag atgttgtaga aggtatgcag tttgatcgtg gttacttatc
    12361 acaatatatg gtaacagata acgacaaaat ggaagctgtt ctagaaaacc catatatctt
    12421 gatcacagac aaaaaaatct ctaacatcca agatattctg ccattgttag aacaaatctt
    12481 gcaacaatca cgtccattgt tgattattgc tgacgatgta gatggtgaag cattgccaac
    12541 tcttgtattg aacaaaatcc gtggaacatt caacgtagta gctgtaaaag ctcctggttt
    12601 cggcgatcgt cgtaaagcaa tgcttgaaga tatcgctatt ttgacaggtg gtacagtaat
    12661 tactgacgat ctaggtttag aattgaaaga tgtaacaatc gaaaacttag gaaatgcttc
    12721 taaagttgtt gtagataaag acaatacaac gatcgtagaa ggttctggag aaaaagaagc
    12781 aatcgaagct cgtgtgcaat tgatcaaaaa ccaaattgct gaaacaactt ctgattttga
    12841 ccgcgaaaaa ttacaagaac gcttagctaa attagcaggt ggtgtagctg ttgttaaagt
    12901 tggtgcagct actgaaacag aattgaaaga attaaaatta cgcattgaag atgcattgaa
    12961 tgctacacgt gccgcagtag aagaaggaat ggtctcaggt ggtggtacag ctctagtgaa
    13021 cgttatcagc aaagtttctg ctgtagaagc agaaggagac gtggcaacag gtatcaagat
    13081 cgttgttcgt gctttagaag aaccaattcg tcaaatcgct gaaaacgctg gttatgaagg
    13141 atcagtgatt gttgataaat tgaagaacgt ggaactaggc actggtttca atgctgctac
    13201 tggagaatgg gtaaacatgg ttgaagcagg tatcgtagac ccaactaaag taacacgttc
    13261 tgccttgcaa aatgccgctt ccgtttctgc cttgttatta acgacagaag cagtcgttgc
    13321 agataaacca gaaccagcag cgccagcagc tcctgcaatg gatccatcaa tgatgggcgg
    13381 tatgatgtaa gaaaaagcaa cttgcttatc tacaactgat cattagtgaa agacagataa
    13441 aaagaggctg gaacagaagc gtttaactcc aagaaataag aagaaattca cggaaattgc
    13501 ttttcaaatt tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgctgtt
    13561 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctcttttttg
    13621 tgtgtaatta taatgaaaat agttattttt aatagttata atattttatc tattaaaagt
    13681 aaatatccat t
//
LOCUS       DACGLH010000078        13581 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.083, whole genome shotgun sequence.
ACCESSION   DACGLH010000078 DACGLH010000000
VERSION     DACGLH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.083"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            132..1754
                     /locus_tag="IXT21_002309"
     CDS             132..1754
                     /locus_tag="IXT21_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869683.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(2021..3190)
                     /locus_tag="IXT21_002310"
     CDS             complement(2021..3190)
                     /locus_tag="IXT21_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ4869684.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            3394..4569
                     /gene="mltG"
                     /locus_tag="IXT21_002311"
     CDS             3394..4569
                     /gene="mltG"
                     /locus_tag="IXT21_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ4869685.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            4705..5184
                     /gene="greA"
                     /locus_tag="IXT21_002312"
     CDS             4705..5184
                     /gene="greA"
                     /locus_tag="IXT21_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ4869686.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            5353..6084
                     /locus_tag="IXT21_002313"
     CDS             5353..6084
                     /locus_tag="IXT21_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ4869687.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            6081..7148
                     /locus_tag="IXT21_002314"
     CDS             6081..7148
                     /locus_tag="IXT21_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4869688.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            7155..7787
                     /locus_tag="IXT21_002315"
     CDS             7155..7787
                     /locus_tag="IXT21_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4869689.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            7927..8592
                     /locus_tag="IXT21_002316"
     CDS             7927..8592
                     /locus_tag="IXT21_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ4869690.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            8620..8913
                     /locus_tag="IXT21_002317"
     CDS             8620..8913
                     /locus_tag="IXT21_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ4869691.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(8981..9928)
                     /locus_tag="IXT21_002318"
     CDS             complement(8981..9928)
                     /locus_tag="IXT21_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ4869692.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(10106..10909)
                     /locus_tag="IXT21_002319"
     CDS             complement(10106..10909)
                     /locus_tag="IXT21_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ4869693.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11055..11969
                     /locus_tag="IXT21_002320"
     CDS             11055..11969
                     /locus_tag="IXT21_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ4869694.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            12017..12487
                     /locus_tag="IXT21_002321"
     CDS             12017..12487
                     /locus_tag="IXT21_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ4869695.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            12572..13180
                     /locus_tag="IXT21_002322"
     CDS             12572..13180
                     /locus_tag="IXT21_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ4869696.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            13314..13514
                     /locus_tag="IXT21_002323"
     CDS             13314..13514
                     /locus_tag="IXT21_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4869697.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4589 a         2049 c         2613 g         4330 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt ttaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
     1021 taggaatctt atctaaaatc acttatataa tgggtataat atctattcta ctttgtacaa
     1081 aaatatttcc atggtttgcc ttacaaaata ctcctttaaa tattattcaa gctccttggc
     1141 gttttatgat attttcaaca ttgttcatat ctttttcagt atctctattt atttctccac
     1201 agttggaaaa attttcttat acaaggagaa gaaattttat actattatta atattagcta
     1261 tttcaatttt aaattattca tcagtagtta acatggtgaa agaaatgaat tttcaaggaa
     1321 cttattctaa tgaacaaatg gaaaataaac ttgaagaatt tgagaatttt gattatgtac
     1381 ctataggcta taaaaaagat caattgcttt tgaaaaacca tcaagttaca gaagatggtc
     1441 aagttattta tagtagcttt tctccaaata aagatgaaat tacatttacg cttaaatctt
     1501 caaaaggcaa aacaatagtt ttacctgtat tccggtataa aggattaacg gttactgtta
     1561 attcaaaaaa tgctacagta ctaaaacaag gaggtccttt tgttacagta aaaagtgaaa
     1621 gtggaataaa taaagtaaca atcaggtatt catacacatt tttttctaaa atctcattat
     1681 ttatttctat aatcacgcta attgttcttc ttttttattg cataaaatta aaaataagaa
     1741 caagccatga atgatttgct agcacatatt ttagcaatct aaagatacta ttttagattt
     1801 aataggccct ttgtcaaata atgttggtaa agagaaatct tagaataatt tggacagaac
     1861 agaaagaaga acacctcttt ctgttctgtt tatttggaat taaagtctgt agcaaaaatt
     1921 gatttttaga tttgtcccac actctttcct acgtttagtc gagtactttt tttaagcaaa
     1981 tgctattcaa agaagtagta tttgctatct tttcatcata tcagctattt gccaacttag
     2041 tagttccttc catcaggtca tagatttggt ttcttagatc agcagtaggc agtttgatag
     2101 ttttgtcagc ttttatattg ctttccatga tgatagcatc ctgtgaatct ttgctaaata
     2161 tagcagatgt atcaaaagag tcgatatttc cttgcgacag gatataatta tctccgctat
     2221 ataccccacc actatatgga tgtccattcg cattttcacg ccataatgag aagagtaagt
     2281 ctttggaaac tagttttcca gataaaactt gtgtaaacag cgtatttaaa tcatagacgg
     2341 acatactgat attaccagtc cctaattcgt tatggatctc tgtttcagat tctgtcagtg
     2401 gtttaaagta atcatcctct ttagacattt gataggatag tgcatgattg gatgaatttt
     2461 tgacttggtc ataaaatttt gtatgctgta atcctaaagg ttcgatgata tcatcggtca
     2521 ataatttttc atacggttga tttgttaatt tgatcaaaat tccagtcaat atggtaaagt
     2581 tcaatgaatc ataatgagag gtctgataat ctgtaaattt taagtgctga atcgcaaatt
     2641 gaacgacttc ttcatcagtc atcggtatgg ttggagaagc agttcggtgc aagcctgagc
     2701 gcatatacag caagtcacgg atagtaattt gctggctgcc agcaatttgt ggatagaatt
     2761 gagccagctt agtatctaaa gaaaggtgcc cacgctcgac ttgttttaaa atcaaaagag
     2821 tagtcaatgc tttttgaacc gaaccaatct gataataaag agaaggatca tttggttgtt
     2881 gcgtgagttt attcgcatag ccatagcctt tttccatgat gatgcgatta tttttcttta
     2941 cgtaaattga gccaatataa tcattttttc ttaatagttg atcgattttt tcaacgagct
     3001 gttgttcatt ggtagtcagt atcaactttt tttgagctga ttgagcatgg gcaactatct
     3061 gagagtgttc attattgtaa aattgccatc caagaatagt tcctgtagag aacgcactaa
     3121 ggctaaacaa cacgacaacg atcattaaaa atgaatattt tcttttttta gcatgttttc
     3181 cttttttcat aatagtttat ttagtctcct ttctttttta cagaaagaat aaagctaaaa
     3241 tagcttgtat cttgataaca tttattttaa tcttatagta atggatttac tacttacatt
     3301 tttgatagaa tatttaaata aaatcattca cgccaattca aatatcatgt ataatgaata
     3361 aagttacgaa cttgatagta aggaggagtt gttttgtcag gaaacaaaca acacccaagt
     3421 aaagataaaa aagaagccag taaggcagct acagcagata taatcgacaa gcggagaaat
     3481 ctgcgtaaaa aagaagataa gattgtgagg aagatcattt tagtgattgc tcttacatta
     3541 ttgattattg gcggatttct tggatttacc gtgtatcgtt atgtagatag cgggttgaag
     3601 cctttagata aatcagatga ccaattagtc caagtcgaga tcccgagtgg ctcttctaac
     3661 aagcagattg gtgagatttt ggagaaggac aatattatta aaagcgggat tgttttcaat
     3721 tattatacaa aatttaaaaa tttaactggc tttcaagctg gctattatca gttagcgcct
     3781 aatatgactc ttgacgagat tggcaaacag ttgcaagaag gcggtacttc tgagccaaca
     3841 aaagttgcag atgggaaaat tgccattcca gaagggtacg atatcgatca gattgctgaa
     3901 cgggtagcga aggtaactgg taaagacaag aaagaatttt tagatttagt gaatgatgag
     3961 actttcttta acgagttgca ccaaaaattc ccagaactgt tggaaagtgc atctaaagct
     4021 gagaacacaa aataccgttt ggaaggttac ttattcccag ccacatatga ttattacacg
     4081 agtacttcgc ttaaggatct agtgattgaa atggtgaaca agacgaatac ggtcatgcaa
     4141 aactattatt cagctatcaa gcagaaaaat ttgactgtac aagaagtttt aactctagct
     4201 tctctagttg aaaaagaagg cgtaaaagaa aatgatcgta aaaacattgc acaagtcttc
     4261 tttaaccgaa tcaaagcaaa tatgccactt cagtcagata tctctgtttt atacgcatta
     4321 ggtgaacaca aagaattagt gacgtatgaa gatacagctg tagattcacc atataatttg
     4381 tataccaata ccggctatgg acctggacct tttgacaatc caagcgaaga ggcaatcaag
     4441 gcagtattag aaccagcaga aaatgactat tattatttcg tagcagatac gagcactgga
     4501 aatgtttatt tcgctaaaac ttatgaagaa catatggaat tagtggaaaa atatgtaaat
     4561 aattcttaga aaaacaggct ctagacattg atcattttta gaaaaacatt tacatttgaa
     4621 gaaaagcgtg gtaatctttt ttggactata ttcctcaaaa gattaccttt tgcggtattt
     4681 tatcgaaaaa aggagacgtt acacatggta gagaaagttt atccaatgac tctagaaggt
     4741 aaagaaaaat tagaaaaaga attagaagaa ctaaaaacag tcaaacgcgg agaaatcatt
     4801 gaacgaatca aaattgcccg cggtttcgga gatctttccg aaaactctga atatgaatca
     4861 gcgaaagatg aacaagcatt tgttgaagga agaatcacaa caattgaaaa tatgattcgt
     4921 tttgctcaaa tcatcgataa cgacggtgta gatgcagatg aggtatcaat tggtaaaaca
     4981 gttacattca tcgaactacc agatggagaa gaagaagagt acacgattgt cggaagcgct
     5041 gaagcagatc cgttttctgg caaaatttcc aatgattctc ctattgcgca agcattgatt
     5101 ggcaaacgta aaaatgatga agtaactatt gctactccag gtggcgatat gttagttaaa
     5161 atcgtcaaag tagaacaagc gtagtctgac tgaaagatct agagagttca tcttactgat
     5221 gatacactct agatcttttt ctctttgtgt attttagatt tcaacaaacg atcttacatc
     5281 taaagtccta tagccaacag ttaaaaaaat aggtatgatg aaatgagaat gtgtcaggag
     5341 ggagaaagag cgatgaatag ttcttggcgt ttttttgtgg tagttgaagc actactgctg
     5401 ttatttgctg tttggcaaat cgtaaacaat actggacttt tgcttttagt gatattcggt
     5461 attttcaata tatatctagc tatgcgcaaa catcctcgta ctaaatttca aaattttcaa
     5521 ttgatattag gaagtttggt catttttttt agcttagtca acagtcctgc tttatggatg
     5581 atggctgtat ttgccgtttt atttattggt ctgaaaggtg tagagatttc agggatagat
     5641 ttaaccaaaa atgctttttg gcgaaagaaa cagatcatga tgatccaaac agagcaatta
     5701 aaaacacata ataatgagcg gaaaagacag caattattcg gtaaccaacg tattggaaac
     5761 gacgtctatg aatgggacga tatcaacatc gccgtcattt ctggtgatac tatcatagat
     5821 ttgggcaata cgcttttgcc aaaagatgat aatattgtga ttgtaagaaa agggataggc
     5881 agaacacgga tcttagtacc tttgggcgta gccatccgat tagagcatgc gacattagta
     5941 ggaaatgtct tgtttgaaga agagcaattt tctttgaaaa atgaacaaat caaaatctac
     6001 agcagtgatt atgatgaaaa ccctcgtaga ttgaagatta ttactaatac cttgattgga
     6061 gatgtcgagg tgatacgaat atgatgggaa aaatatccag agcgatgcta gctgtttatt
     6121 cggggattgc ggctttcctt attattctat tttcactttt cacttatttt tatgccagca
     6181 atcaaagtca ttggtgggga gaattactgc gtgcacgttt attatatgtt ccgcttatct
     6241 tccatttgtt agccatttcc ttaggtgtag gattaattgt ctttctatta ttatcactta
     6301 ttcaaaagac aaaatacggg aaaatcgaag aaaagctgcg tgcactttct tctggcaatt
     6361 atgaatccaa attgttgctt cttccgatcc caagtgcatc ggacgattta tacatcaaag
     6421 atattgataa ggaaatcact aagataaaag aaaaaatgat tgaagtatct agtgaattac
     6481 aaattgtaac gagccgtccc caatatgtag atggacaaac caaagaagaa attttagaat
     6541 tagaaagaca tcgattagcc cgtgagctgc atgattcagt ttcgcaacaa ttgtttgcag
     6601 caatgatgat gatgtcggca ttgacggagc aagcagaaaa aagcgagaca ccagaaatgt
     6661 ttcgcaagca gttgaaaatg gtagcagaga tcatcaacgc ttcccagtct gagatgcgtg
     6721 cgctgcttct tcatctacgt ccagtcaatt tagaagagaa aagtttaaag caaggtatcg
     6781 agcagttatt gaaggaattg caaaataaaa tccagatttc gctgaaatgg gatgtagaag
     6841 atgtaaaact atctagttcc attgaggatc acttgttccg aatcgttcaa gaattgttat
     6901 caaatacatt aagacatgct aaagccaatg aattagaggt atatttgcac aaaatagaca
     6961 ataatctttt gttacgtatt attgacgacg gaacagggtt taatatgaat gaaacgaaaa
     7021 cgggaagtta tggattgaac aatatcaaag aacgagtagc tggtattggt ggaacagtaa
     7081 aaatcattag ttttaaaggt caaggtacaa gtgtagaaat caaggtccct ttgatgaagg
     7141 aggcatagat aaggatgata aaagttttat tagtagatga ccatgaaatg gtgcgcttag
     7201 gcgtctcttc ttatttatct attcaagaag acattgaagt aattggagaa gctgaaaatg
     7261 gacgacaagg ctatgagaag gcgatgacac ttcgaccgga cgtcatttta atggacttag
     7321 tcatggaaga aatggatggt atcgaatcaa caaaagcaat cttaaaagat tggccagaag
     7381 ctaaaatcat tatcgtaacc agttttattg atgacgaaaa agtttatcct gccattgaag
     7441 caggtgcagc agggtatttg ttgaagacct ctaccgcaca tgaaattgct gatgctataa
     7501 gggcaacgca acgtggcgag cgagtattag aaccagaagt gacaacaaaa atgatggaaa
     7561 aaatgagtcg gcgaaatgag cctgtattac atgaagaact aacgaatcgg gaaaatgaaa
     7621 ttttaatgct gatttctgaa ggaaaaagta atcaggaaat agcggacgaa ttgtttatta
     7681 cactgaaaac agtaaaaaca catgtttcca atatattggc taagctagaa gtggaagacc
     7741 gtacacaagc cgctatctat gcattcaaac atggtttggt gaaataaaaa cgatgataaa
     7801 gaggataatc aaaagctgag aaaataacta ttatttttct tggctttttt acaataatga
     7861 tgcttgcttt tgtttttgta aaggctttgt tatactgaga aaagaagata ctaaaggagg
     7921 aaaacggtga aacaaaattt tgcaattgta ggtcttggac gatttggcgg aagtatctgc
     7981 cgaacactga tcgaagctgg tcaagaagta ctggctattg atagtaatga agatcgggtc
     8041 aatgaatata tgaatatagc cactcatgct gttgtggcaa atgcacagga tgaaatgaca
     8101 cttcgatcat taggcataag aaatttcgat catgtaattg tcgctattgg agaagatatt
     8161 caagccagta tcttagttac tctgatggta aaggaaatgg gcgtaccaaa tattttagca
     8221 aaagcccaaa atgagtatca tgccagagta cttgaaaaga tcggagctga tcgcgtcgtc
     8281 catcccgagc gagacatggg aataaggatt gcgcataatt tagtgtcaaa gaatatttta
     8341 gattacctag agttgtcaga taaatattct ttagcagaaa tccgagtctc caatcctaaa
     8401 tttttcaaca aaacattagc tgaattaaat ttcaggcaac gttttgactt aacagtagtc
     8461 gccatacgaa gagtagatca aacggttatc gcttctcctg cagcagatga atatgttcgt
     8521 gaaaatgata atctactggt tattggagag acaggcgatg tggatattct agacgataag
     8581 atgaatcaat agaaagaaaa gagaaagtag gttgttggga tgaaattaac ggtaacgcca
     8641 aaagcacaaa agtggtttga ctcagaaatt gatctgccag aaacttacgg aatacgtttt
     8701 tttggaaaag tctacggcaa aacagaggtt catgatggtt tttcgattgg aatgtcagtt
     8761 gaacagccag aacatcctgt aaagaaagtg gaaattgatg ggacactttt ttttatcgag
     8821 gaagcagatg aatggttctt taaaggatat gatttacttg ttgattatga tcccaacctg
     8881 gaggagccaa cctatcactt taaaaaacaa taaaaaaaga gagggtgaca caagttttgt
     8941 caccttctct tttttctttt gaaaatattg ttttcagcta ttaatatcct gcagacaatt
     9001 ttacttgatt ggtttgaagc tcatgggtgg caaagtagct tttcagattg gttaaaaaaa
     9061 tagccattaa tttcttttga aaatcaggtg tcaaacctgc aatgtgggaa gtaatcagta
     9121 cattgtccat tttccaaaga ggatgatctt gtggaagagg ctcttcctca aaaacgtcta
     9181 atgctgcaaa ggaaagttgc tggttattta aagcctgcac cagatcttgt gtcttaacag
     9241 aagcaccacg gccaacattg atgaatacag attttggatc aaagtgttca aacatatgct
     9301 gatcaaagag cccagttgtt tcctccgtta gaggtaagat attgactacg aaatctactt
     9361 caggaagaac ttcctttagt tgatttaaag aaaaagtctt ttccattcct tctgctggat
     9421 gaccggatgt attgattcca tagcagttca tgtctaatgc tttgcatttt tttgcaatca
     9481 tttttccaat atggccagtt ccaatgatta gtattttttt cccttggata gattggtaat
     9541 ggaccgatga acctaaccat ttcatgtcac tctgtgcctt ttgtgcttga aaaagaccac
     9601 gagaataccc aaggatgatt cctaagacat gctccgtaat cgattcactg tggatgccac
     9661 taccatttga aagtaaaata tgtttctttg aaaaagtctc aaggggtagc tgatcaacac
     9721 cagctgagat agattggacc catttcaagg tagaagaggc taatagtttt tcttggtagt
     9781 ctgagttcca gcccaaactg atctctacat tttgtagatc ctctggttgt aattctgttt
     9841 taaagatata accaggcgct atagtatgga tttcttcgat aaattcatca cgaaatggac
     9901 gatctgcata aatgattctt tctgacatag ttaatttccc gcctttcttt actctaagtg
     9961 tagcgcatac aagaagaact ttgaagcgcc ttgcttgttt tttgagaaag aaatattttt
    10021 caaataaaaa aggggctgtg acaaaaaaat gtcacaacct ctaaaagggt ctattgaagt
    10081 agaaataacg agttgtcttt atttcttaat tgatcgatat ttttgcccat cctttgacag
    10141 cttgagcaaa aataaagatt cctgcaaaag caatgaaggc gctagtgtca aaagaaggaa
    10201 gaaagaccat caagattgtt gcaattatag taggcaatga aattgaatag atcaatgttt
    10261 ttaaacaatc aagaaaagta gcttttgtta gtcgaaagcg tgcatagaga aaagcagcaa
    10321 tgttggtaaa tagcaaagtg atcagcagat tcagaaaact aggataaata gaaaatataa
    10381 atgtcaggac ttttatccaa agtggtatag atgcttcact taaaaaagaa cggagctgct
    10441 tacctgttaa gttttttaaa gattcattgg tgtagggaag ttctaattgg ttattgttca
    10501 acaaagcaga agtcgtgcct gtattaggaa gagctatgat aagtttgttt tttaacatcc
    10561 cgacactaac aaaattcccc atcaaatcag tagagatgtc ctgttctgtt cgttttccct
    10621 ctggatcaaa tgtgaagata atggagtttg tttggtaaat gaatccttct ttttcttcgg
    10681 tttgaagctg cccgttttgg attgtaaaat caggaatgcg agtagcaatc ttttgcccat
    10741 ccaattgaat gttgtttaag acttgaaaaa cttgaaaaga gattggcaaa gccataatta
    10801 tactcaaaac taaaagatag acaatggctt tccaaaatgg gacatttctc gcctccttta
    10861 ggtctttgaa tcgtataaag gaactgataa ttaattgttt tgttgtcata agtactcctc
    10921 atctagtagt gtcatcactt gcaattctag cgaagtatca agtgaaatac aagtgcttta
    10981 aggaaacagc tgaatgattg gtgaaatatc aaaaaaatgt tattcttgtc taaagactat
    11041 ccaatataaa taaaatggag gaaggaatag tgcctacaca gccacagaga tcatcaaaaa
    11101 aactatttgg agtagtggtt ttgaatgcgg tgatcacttt atcagaattt attggaggga
    11161 ttatgtcagg gagtttgtct cttgtttcag atgctttcca taaccttagt gatacattgg
    11221 caattatttt ttcttacggg gcacaaaaaa tggcccgaaa agaagcaaat aaaaaacgta
    11281 cgtatggcta ccagcgttta gaaatattga gtgctttcat caactcattc atattgatca
    11341 tattatcctt gtttttaacc gttgaagcat tcaagcggtt cacttcacca gaaaagataa
    11401 atagtcattt gatgctaaca gtagcagtta ttggattgct cgcgaatttg ttctcagtat
    11461 tattattacg acaagaagca gatgaaagtt tgaatataaa atctagttat ttgcatttgt
    11521 taagtgatac actgtcttcg gtaagtgtga ttatcggagc aattttgatc cgttttttcg
    11581 atatctactg gatcgatcca gttattacac tgatcatttc gatatatata ttgatagaag
    11641 cgataatggt gatcaaaaaa gctgccgcta ttttgataca aagtgcacct gctatcgact
    11701 atgaaaaaat ggaacaagaa attgaagcaa tcgatggggt aaaggatgtt catcatgtcc
    11761 atatttggca gtatagcgaa aagataatta ttttcgacgg acatattgat tttgaagatc
    11821 agctactaag cgagattgaa aagatatatc ctcgtataat ttctttatta aaattaaaat
    11881 atggtattac acatgtgacg atccaagcag agacaaaggc aaaggatcaa aaaaaattaa
    11941 tttttttaaa taaagatgaa agttattgaa atatagagta tgataaataa gaatttttaa
    12001 taaggtaggt tggctcatga agcagtatcg acagttaaaa ggatatttag aagcaaaagg
    12061 atattgggag atactagtct atctcttttt tggtggtttg gctacgattg taaattttgt
    12121 tagttttgct ctcgcacgcc aatattttga tttaagtatg gccttatcca attctatttc
    12181 gtggttttgt tctgtattgt ttgcttttgt aacgaacaaa ctttgggtat tccattcaaa
    12241 atcaccgaat tttacacatg cacttattga atgcggtaag ttcttcttct accgtatcct
    12301 ttcttacgga ttggacatgg gggcaatggt attgttgatc aatgttatga acagcaatga
    12361 gtatgtggcg aaaatcatca ctcaaattat tgttatcctt gcgaactata ttttcagcaa
    12421 gttgtttatt tttaaagaaa ctgaggtttt tgaagaagaa gtaggtcaaa gtgaaaaaga
    12481 cgactaggca taacaattga agaatttcct tctctttgtt actctttgta atgtagacat
    12541 tattaagaaa acattgagga ggaatttttt tatgacttac acattaccag atctacctta
    12601 tgcatatgac gcattggaac cttatattga cgaagaaacg atgcatctgc atcatgataa
    12661 gcatcacaat acttatgtga cgaatttaaa ttcagcaatt gagaaatacc cagaattagg
    12721 cgaaaaaaca atagaagaat tattatctga tatggacgct attccaacag atatcaagac
    12781 agctgtacgt aacaatggtg gcggacatgc taaccattca tttttctggg aaatcatggc
    12841 accaaatgct ggtggcgaac ctacaggaga aataaaagaa gcgattaatg aagcttttgg
    12901 tgatttttct tcttttaaag aagaattcaa aaaagcagcc gctggacgat ttggttctgg
    12961 atgggcttgg cttgtaatgg aaaatggaaa attagctatt acctctactg caaatcaaga
    13021 ttctccattg atggaaggaa agacaccaat tctaggtttg gacgtttggg aacatgctta
    13081 ttatttgaaa tataaaaatg tccgaccaga ttatattgca gcattctgga atgttattaa
    13141 ttgggatgaa gtaaacaaac gtttcgaagc agcaaaataa ccatttcctc cgtcatatca
    13201 gtcttaaaaa taatttggaa aataaaagag cgaaagcttg ctagcgtttt ctaaattggg
    13261 tataatgttc ttgcaaggtt acttgcaaga acttttttgg aggatataca tttatggaac
    13321 acggaacggt taaatggttc aacaatgaaa aaggatttgg gtttattaca gtagacggcg
    13381 gagatgacgt atttgttcat ttctcagcaa ttcaaggaga cggcttcaag agcctagaag
    13441 aaggtcaaga agtagaattc acgattgttg aaggtgcacg cggacctcaa gctgctgaag
    13501 tgactaaatt ataataattt gttgaataaa gacacgactt cttagagagt cgtgtctttt
    13561 tgtctattta tgaagttttt a
//
LOCUS       DACGLH010000079        13488 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.084, whole genome shotgun sequence.
ACCESSION   DACGLH010000079 DACGLH010000000
VERSION     DACGLH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.084"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(164..388)
                     /locus_tag="IXT21_002324"
     CDS             complement(164..388)
                     /locus_tag="IXT21_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ4869698.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="IXT21_002325"
     CDS             complement(586..2166)
                     /locus_tag="IXT21_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ4869699.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="IXT21_002326"
     CDS             complement(2567..3943)
                     /locus_tag="IXT21_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ4869700.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="IXT21_002327"
     CDS             complement(4071..5189)
                     /locus_tag="IXT21_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4869701.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="IXT21_002328"
     CDS             5331..5765
                     /locus_tag="IXT21_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869702.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="IXT21_002329"
     CDS             complement(5843..6376)
                     /locus_tag="IXT21_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ4869703.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IXT21_002330"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IXT21_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ4869704.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IXT21_002331"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IXT21_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ4869705.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="IXT21_002332"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="IXT21_002332"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ4869706.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYVLPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="IXT21_002333"
     CDS             complement(10101..10679)
                     /locus_tag="IXT21_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ4869707.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="IXT21_002334"
     CDS             11001..11471
                     /locus_tag="IXT21_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ4869708.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="IXT21_002335"
     CDS             complement(11579..12805)
                     /locus_tag="IXT21_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ4869709.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3959 a         2718 c         2248 g         4563 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgt actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaa
//
LOCUS       DACGLH010000080        13427 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.085, whole genome shotgun sequence.
ACCESSION   DACGLH010000080 DACGLH010000000
VERSION     DACGLH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.085"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..912)
                     /locus_tag="IXT21_002336"
     CDS             complement(<1..912)
                     /locus_tag="IXT21_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ4869710.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="IXT21_002337"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="IXT21_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ4869711.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="IXT21_002338"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="IXT21_002338"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ4869712.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="IXT21_002339"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="IXT21_002339"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ4869713.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /locus_tag="IXT21_002340"
     CDS             4438..5319
                     /locus_tag="IXT21_002340"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ4869714.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /locus_tag="IXT21_002341"
     CDS             5316..6032
                     /locus_tag="IXT21_002341"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ4869715.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="IXT21_002342"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="IXT21_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ4869716.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="IXT21_002343"
     CDS             6749..7750
                     /locus_tag="IXT21_002343"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ4869717.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7747..9108
                     /locus_tag="IXT21_002344"
     CDS             7747..9108
                     /locus_tag="IXT21_002344"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ4869718.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9068..10219
                     /locus_tag="IXT21_002345"
     CDS             9068..10219
                     /locus_tag="IXT21_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ4869719.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="IXT21_002346"
     CDS             10194..11363
                     /locus_tag="IXT21_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4869720.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="IXT21_002347"
     CDS             11538..12917
                     /locus_tag="IXT21_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4869721.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13198..13427
                     /locus_tag="IXT21_002348"
                     /pseudo
     CDS             13198..13427
                     /locus_tag="IXT21_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2922 g         3682 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg aaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gaggaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcaaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg
    12961 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggctggac
    13021 ttattaccgc agatgcaagc ttttgaacac cgtttatttt caaaaaaggg actgtgacac
    13081 gatttgtgta ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt
    13141 ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt atttggatgg
    13201 caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaaatt
    13261 cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt
    13321 ttctctatca ataaaaagaa agttcagcgc ttgatcaaga agctaggaat aaaagtaacg
    13381 gcatacacgc gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DACGLH010000081        13404 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.086, whole genome shotgun sequence.
ACCESSION   DACGLH010000081 DACGLH010000000
VERSION     DACGLH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.086"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            79..1125
                     /locus_tag="IXT21_002349"
     CDS             79..1125
                     /locus_tag="IXT21_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ4869722.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IXT21_002350"
     CDS             1474..2106
                     /locus_tag="IXT21_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ4869723.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IXT21_002351"
     CDS             2694..4133
                     /locus_tag="IXT21_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4869724.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IXT21_002352"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IXT21_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IXT21_002353"
     CDS             4895..5110
                     /locus_tag="IXT21_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869725.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IXT21_002354"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IXT21_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IXT21_002355"
     CDS             complement(5770..6681)
                     /locus_tag="IXT21_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4869726.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IXT21_002356"
     CDS             complement(6700..7704)
                     /locus_tag="IXT21_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4869727.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IXT21_002357"
     CDS             complement(7701..9824)
                     /locus_tag="IXT21_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4869728.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IXT21_002358"
     CDS             complement(9829..12276)
                     /locus_tag="IXT21_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4869729.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IXT21_002359"
     CDS             complement(12263..12652)
                     /locus_tag="IXT21_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4869730.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IXT21_002360"
     CDS             complement(12712..13215)
                     /locus_tag="IXT21_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4869731.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..>13404)
                     /locus_tag="IXT21_002361"
     CDS             complement(13228..>13404)
                     /locus_tag="IXT21_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869732.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4099 a         2643 c         2454 g         4208 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaacccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DACGLH010000082        13335 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.087, whole genome shotgun sequence.
ACCESSION   DACGLH010000082 DACGLH010000000
VERSION     DACGLH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.087"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IXT21_002362"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IXT21_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ4869733.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IXT21_002363"
     CDS             complement(738..1460)
                     /locus_tag="IXT21_002363"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ4869734.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IXT21_002364"
     CDS             complement(1596..2477)
                     /locus_tag="IXT21_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ4869735.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IXT21_002365"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IXT21_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ4869736.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IXT21_002366"
     CDS             3717..4196
                     /locus_tag="IXT21_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ4869737.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IXT21_002367"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IXT21_002367"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ4869738.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAISSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IXT21_002368"
     CDS             5497..5625
                     /locus_tag="IXT21_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ4869739.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IXT21_002369"
     CDS             5629..6174
                     /locus_tag="IXT21_002369"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ4869740.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IXT21_002370"
     CDS             complement(6628..8319)
                     /locus_tag="IXT21_002370"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ4869741.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IXT21_002371"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IXT21_002371"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ4869742.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IXT21_002372"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IXT21_002372"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ4869743.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IXT21_002373"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IXT21_002373"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ4869744.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="IXT21_002374"
     CDS             12601..13302
                     /locus_tag="IXT21_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ4869745.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3752 a         2710 c         2366 g         4507 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttaacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgata tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacatga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaac aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taaat
//
LOCUS       DACGLH010000083        13112 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.088, whole genome shotgun sequence.
ACCESSION   DACGLH010000083 DACGLH010000000
VERSION     DACGLH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.088"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            154..1650
                     /gene="glpK"
                     /locus_tag="IXT21_002375"
     CDS             154..1650
                     /gene="glpK"
                     /locus_tag="IXT21_002375"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ4869746.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            1664..3487
                     /gene="glpO"
                     /locus_tag="IXT21_002376"
     CDS             1664..3487
                     /gene="glpO"
                     /locus_tag="IXT21_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ4869747.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            3488..4204
                     /locus_tag="IXT21_002377"
     CDS             3488..4204
                     /locus_tag="IXT21_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ4869748.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            4510..6438
                     /locus_tag="IXT21_002378"
     CDS             4510..6438
                     /locus_tag="IXT21_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ4869749.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            6564..7118
                     /locus_tag="IXT21_002379"
     CDS             6564..7118
                     /locus_tag="IXT21_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ4869750.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(7603..7917)
                     /locus_tag="IXT21_002380"
     CDS             complement(7603..7917)
                     /locus_tag="IXT21_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ4869751.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(7931..8191)
                     /locus_tag="IXT21_002381"
     CDS             complement(7931..8191)
                     /locus_tag="IXT21_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ4869752.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            8361..8675
                     /locus_tag="IXT21_002382"
     CDS             8361..8675
                     /locus_tag="IXT21_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ4869753.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            8677..10332
                     /locus_tag="IXT21_002383"
     CDS             8677..10332
                     /locus_tag="IXT21_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4869754.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            10415..12550
                     /locus_tag="IXT21_002384"
     CDS             10415..12550
                     /locus_tag="IXT21_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4869755.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            12725..13090
                     /locus_tag="IXT21_002385"
     CDS             12725..13090
                     /locus_tag="IXT21_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869756.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYAVCIIKRDK"
BASE COUNT         4384 a         2246 c         2859 g         3623 t
ORIGIN      
        1 aaaaaagaaa agaattgatt aaaaaatagc ttcataataa aaaactgtca aaaaaagaaa
       61 aaaagaataa aattttgaca gtttcttttt ttaaaacagt agtatgataa gagtagttaa
      121 agaaagcgtt tgcatataat ggagggaatc acaatgacag aatcaacgta tatcatggcg
      181 attgaccaag gtacaactag ctcacgagca attatctttg acaagaaagg aagacacatc
      241 ggcagttctc aaaaagagtt cacgcaatat ttcccgcaag aaagttgggt ggaacatgat
      301 gcaaatgaaa tctggaattc tgtacagtca gttattgccg gagcgttcat cgaatcaggg
      361 atcaaaccaa atcagattgc tgggattggc ataacgaacc agcgtgaaac aacagtaata
      421 tgggaaaaag atacaggacg tccaatctat catgcagttg tttggcaatc cagacagtca
      481 gctggaattg cagataactt aaaaaatgaa ggctatcaag aatttttcca cgaaaaaaca
      541 gggttagtga tcgatgccta tttctcagca acgaagatac gctggattct tgatcatgta
      601 gaaggcgcgc aagaacgagc tgaaaaaggc gaattgatgt ttggaacgat cgattcttgg
      661 ttagtatgga aactgaccga tggacaagca catgttactg attactcgaa tgccagtcgg
      721 acgatgttgt tcaatatcca tcagctagat tgggatcagg agattttaga tttactaaat
      781 attcctcgag tgatgttacc aaaagtcact tctaactcag aagtttatgg ttatacgcaa
      841 ggttatcatt tttatggaag cgaagcacca atttcaggga tggcaggaga ccagcaagcc
      901 gcattattcg gacaaatggc attcgaacca ggcatggtca agaatactta tggtacaggt
      961 tcatttattg tcatgaatac cggtgaaaaa ccgcaattat ccaaaaataa tttattgacg
     1021 acaattggtt acggcataaa tggaaaggtt tactatgctt tagaaggaag tatttttgtg
     1081 gctggttctt cgattcaatg gctgagagat ggacttcaaa tgttgcaaaa agctagcgat
     1141 tcagaagacg cagcgaaacg ctctacaagc gaagatgagg tttatgttgt tcctgcattc
     1201 gttgggctag gtgctcctta ttgggatcag gcagcacgag gggctatgtt tggcttgaca
     1261 cgtggtacga caaaagaaga tatcattaaa gctactttac agtcgattgc ttatcaagtg
     1321 agagatatca tcgatacgat gcaagatgac actggaatca agattcctgt attgaaagta
     1381 gatggcggtg cagcaaacaa tgaatatctg atgcaattcc aaacagatat cttgaatgta
     1441 ccgatccaac gagcagaaaa cttggaaaca acggcgctag gggcagcatt tttagcagga
     1501 ttagctgtcg gttattggaa agatacagac gagatccgcg aattttatga agctggcaag
     1561 ctatttgaag ttcaaatgga ggaagagcgc cgagaaaaac tttacaacgg ttggaaaaaa
     1621 gcagtaaaag ctacccaagc atttgaataa ggaggaaatc catatgtttt caaacaagac
     1681 aagacaagat agcattcaaa aaatgcagca agaagaattg gatctgttga tcatcggtgg
     1741 cggaatcact ggtgccggtg tggcagtcca gtcagcagca tcaggaatca aaacaggatt
     1801 gatcgaaatg caggattttg cagaagggac ttcctctcgc tcgaccaaac ttgttcatgg
     1861 cggtattcgt tatctgaaga catttgatgt ggaagtagta gctgacacag ttggtgaacg
     1921 tgcagtcgta caaagtattg ccccacacat cccaaaacca gatccaatgc ttttgccaat
     1981 ctatgagggc gaaggagcaa caaccttcaa tatgttctcg gtcaaagtag caatggacct
     2041 ttatgacaaa ctggcaaatg tgacaggaac caaatatgag aattatacgc tgacaccaga
     2101 agaagtattg gaaagagaac cgtttttgaa aaaagaaggg ctaaaaggcg cgggtgtcta
     2161 tctagatttc cgcaacaatg atgcccgttt agtgattgat aatatcaaaa aggctgccga
     2221 agatggggct tatctagtaa gtaaaatgaa agcggttggc tttttatatg atggcgatca
     2281 aatcgttggc gtcaaagctc gtgacctgct gacagatgaa gtgatcgaaa tcaaagcaaa
     2341 attagtgatc aatacgagtg gtccttgggt agataaagta cgaaacttga attttacgcg
     2401 tccagtctct cctaaaatgc gtccaactaa agggatccat ttagttgtag atgcgaaaaa
     2461 actgcctgta ccgcaaccca catacttcga tacaggaaag caagatgggc ggatggtttt
     2521 tgctattcca agagaaaaca agacttactt tggtacgaca gatacggatt accaaggaga
     2581 ttttacggat ccgaaagtca cacaagaaga tgtagattat ctattggatg tgatcaatca
     2641 tcgctatcca gaagcaaata tcacattgaa tgatatcgaa gcaagctggg caggacttcg
     2701 tccactgttg attggtaatt ctggttctga ttacaatggt ggagataatg gatcgatttc
     2761 agacaagagc ttcaataaag ttgtcgatac agtaagtgaa tataaggaaa acaaagtttc
     2821 tcgtgctgaa gtagaagacg tgttgaacca tttggaaaac agccgtgatg aaaaagcacc
     2881 ttctacgatt tccagaggta gttctttaga aagagaacca gatggtttgc tgactttatc
     2941 tggtgggaaa atcaccgatt accgtaagat ggcagaagga gctttgcgat tgatacgcca
     3001 attattaaaa gaagaatacg gaatagagac gaaagaaatc gactctaaaa aatatcagat
     3061 ttcaggtgga aacttcgatc caactaaatt agaagaaaca gtgacagaat tagcaaaaga
     3121 aggagtagca gccggtttag aggaagaaga tgctacttat atcgctgatt tttacgggac
     3181 taatgcccga cgtatctttg aattagcaaa agaaatggca ccttatcctg gtttgagtct
     3241 cgctgagtca gctcggttac gttatggttt agaagaagaa atggttttgg ccccaggcga
     3301 ttatctcatt cgtcgtacaa atcatctgtt gtttgaacga gatcagcttg atgagatcaa
     3361 gcaacctgtg atcgatgcaa ttgctggata ttttgaatgg acagaagagg agaaggcgca
     3421 acagactaaa cgtttagaag cattgattgc agaatcagat ctgcgggaac taaaggggga
     3481 gaaataaatg aacagtgata tgactcaaat catgggggag tttattggga cactgatcct
     3541 agttttgctt ggagatggtg tgtgtgcggc tgtgaattta aacaaaagta aagcacaggc
     3601 ttctggctgg attgtcatcg catttggttg gggactcgct gtaacaatgg ctgtctatat
     3661 ttctggattc atgggcccag ctcatttgaa tcctgcagtc tctctagcta tggcgatgac
     3721 aggggcaatt agttggaatc ttgtcgttcc tttcatcatt gcgcaagttt taggagcttt
     3781 tgcaggggcc attcttgttt ggctttctta tttgccacat tggaatgcaa cgaaagatga
     3841 aagcgcgatt ttaggaacgt ttgcaacagg tcctgctata cggaattatc cagcaaatgt
     3901 gatcacagaa ttaattggta catttgtatt agttcttggg ttgctagctt ttggacaaaa
     3961 cgaatttgca cctggaacga acgtattcgc tgtaggtgga ttgatcttag ccatcggtct
     4021 ttcattagga gggccaacag gatatgcaat caaccctgct cgggactttg gtcctcgatt
     4081 agcacatgcg gtattgccaa tagctaataa aggaacttca gattgggcat attcatgggt
     4141 accaatcgca ggtccaatga tcggagcaat tatcgcggta ggtgtctatt cattaatggt
     4201 gtaaatctat aaaacatact aaagtttgac aaccaaaatg tgtcttgcta tagttaagtg
     4261 gaaaataaat aagacctaga agaatggggt atgttttgtg agtgtagctg gtatctaatc
     4321 agtaagtcga atagccaata aacaaagttt atttggtgtg cttatgaaac ggtacctgtc
     4381 aggaaaacaa tatctttgta ttctaataag gataggacat gactttttta agctgtgttc
     4441 tatcctttta tttatctcta ggcccttatt tattttggga caggtatctc ttcaagaaaa
     4501 ggagagaata tggacaacga aatgattagt caattacaat ttgatcggat caaaaaggaa
     4561 atccaagcaa gagcaattgg aaactatagc aaaaaacgaa tcagcgatat gacggtctcg
     4621 acaaacctac aaactgtctt ggatcgtcaa gaagaaacaa gagaagcacg cttgatctta
     4681 gaaagcagtc agcatgttcc atttatgggg ttgccgagga tcgatgcact gacagaacaa
     4741 gtcaaaaaag ggttgattct gcagccaacc gatttgatcg aatatgcgga ttttcttaga
     4801 agcagtcgga tgatcacaaa gttttttgat aagaatcaat accagacacc cttgttgttt
     4861 gcttatagca aacatcttcc tgatttgata aatgtagaag agttgattga ccagaaaata
     4921 aaaaataata aagtcagtga cgacgcgtca cgtaatttgc gtaaagtacg caaacaatta
     4981 caactaatcg aaaaagaaat ccagtcaaaa ttactgaaat tcttgcgtca tccgaaaaac
     5041 aaagaaatga tccaagaagc aatgattgtt caaaaaggcg aatactatac gattcctatc
     5101 aaagccagct ataaaaataa aatagatgga acgattatcg atgaatcaaa taaaagaacg
     5161 acggttttta ttgaaccaac tgtgatttct aaattgaatg aacactatca gttgttaaaa
     5221 gctgaagaaa tcagtgaaga atatcaagtc ctggcggcat tgactggtgc tatcgcagaa
     5281 aacgaagaag tgatcgattt attgatcgaa acgatcacgg tgttagatat catctttgct
     5341 cgggcaaaat ttagccgaga aatcaatgga agcacaccta gaatcaataa atcggaacat
     5401 atcattatca agcaaggcag acatccattt ttacctgagc atgcggtacc attggatgtt
     5461 gaaatcggta aagactatcg aggcttgatt atcaccggag caaatgccgg aggcaaaaca
     5521 gtcgtgttga agacagtcgg tttattgact ttgatggcga tgttcggtat gcaagttccg
     5581 gcaaaagaag ggacagagct tgctgtcttt gatgagattt tcgtcgatgt cggtgaccac
     5641 caaaatttag aaaatgcgtt gagtacattt tctggtcata tgcaaaacat cgctgctatt
     5701 ttgaaaaaga taaaaaggaa tacgttggta cttttagatg agattggcag tggaacagag
     5761 ccaaatgaag gggctgcact agcgatcgca atcatggaat ctatgtatga gcaaggggca
     5821 ttgatcattg ccacgaccca ttatggagaa atcaagaaat tcgccggaga tcatgaagat
     5881 tttgtgccgg ctgctatggc tttcgatcgg gaagcacttc gtcctaaata tcagttgcgg
     5941 gttggagaaa caggagaaag ccaagctttg tggattgctc ataaaatgga tatgtcgatg
     6001 aaattgatcc atcaagcaga gcgttacttg gcggaaaaag cctatcggac acagaaaaaa
     6061 gaatttcttc ctaagacagc agttacaaat aggaaggaga ataaaaaaga acgccagctc
     6121 tttgcaaaag gtgatcggat atttgtcaat gaataccaaa aagaagcgtt agtttatgaa
     6181 gatataggtg aagatacgat tgatgtatat ttagacaaaa aaataatcca cgtgcctcgt
     6241 cagcgtgttc gtctggtgcg ttcagcagaa gatctgtacc cgacaggtta cgatttagac
     6301 agtctgttta tcgattataa aacacgtaaa cggcagcgtg acctagagcg aggatcaaaa
     6361 aaagctcata aagtactagt aaaagaaatg cgcaaacgac aggaagaaag aagagtaaac
     6421 gatgaaaaca gcaagtgatt atctcaaatt agctcataat atcagtggct gtaaagaaaa
     6481 ataaaaacag tgaaatcatt ggttttattc cccgtgtaag ctaaaataaa aagaaaagag
     6541 tgctgaggag gtagggcgat aaaatgaaat taagcacaga ctggcgtaaa gaaatacaaa
     6601 cgattccgaa tcttttatcg atattccgta tttttttgct gccgatttat ctttattttg
     6661 tgctgagaca gtcattttat atagcaggag cggtcattgt tgtctcaggt ttgtctgatt
     6721 atctagacgg ggtcattgct cggagatata atcaggtaac agacttaggg aaggtgcttg
     6781 atccgtttgc agataaattg acacaacttt ttctgatttt gtctatggca tggtatcgac
     6841 cgtggctttg gctgttgttt ggtctattct tgataaaaga aggctttatg tttgtagctg
     6901 gattaatcgg gctatctaaa aatatcaaat taagtggagc gaagtggtat ggcaaagtag
     6961 cgactgctgt catctatgtt ggcatgatcc ttttgctgct tttcccagaa ttaccaacgc
     7021 tgtgggtgag agtcattttt gccgtcatca cttacggtct tttacaatct tttgtcttgt
     7081 atgctgtcga gtaccgaaag atgtttcaaa gaaaataaaa aaggggtgtg acctatgtcg
     7141 cattcctttt tttgataaat agactcccag tagctacttc tcgataataa gccaaccaaa
     7201 ccaaaaacag gagaagttgt ttttgaaatt atttcttgtg attcggagtt aaatggtagg
     7261 ctcacaacct cttggtaaac ggtgttcaat cagctgacct tcggtattag ctcaaaaaac
     7321 ggcaaaatat gaggagctat tttcccgttt ttttctctaa tacttaggtc aaaccgctga
     7381 tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccaac
     7441 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg atgcaggaca
     7501 gcgttttcac aacctttgat tcacggtttt ccagaagtag cttcttcgaa aaataagccg
     7561 aaatttcttg aagctgatca cttctgtcgc aacctctact gactaaggct tgatataagc
     7621 ataaccttgg gattgcagtt caactaaatc aaggacacct gctggtacaa cgattacttg
     7681 ttcaggtaaa gaactttctg aaatatgatt agcacgcata gcattggcgc aagcgtgaaa
     7741 aacgactcct tgcaaatcta agaattctgc attggcagag tctagataac cggtaatcgc
     7801 gatgccattt acagatacga caatcgtcgt ttctggagaa gcttttatca ggttttttac
     7861 gtttttccct gtttctgacc atttttctaa ttcatcaata tggaaaacga ctttcattga
     7921 aatcattcct ttacttttta ataatcatct tgatcgcttg ttctagttta gccgcttgtt
     7981 cttctggatt actatcaggt ttctcaaagc aatttttcag attatctgca acgatcatgc
     8041 ccattacgcg atcaatacta gaacgaacag cgcttaattg agtgatgaca tccatacatt
     8101 ctttttcgtc ttcgatcatt ttttggatac cacgaatttg tccctctgta cgtttcaaac
     8161 ggttcaggat atttttatta tcaatctcca taacacattc tcctctattt ttataattag
     8221 ataatacccc ttatcggtat tttatgcaaa tataaaaagt agtgaaaaaa atgaagagtc
     8281 attctggttg acatattaat aaagctcctg tttaatatac cccatgaggt atttataaaa
     8341 atgtaaatga ggtgcagaaa atgaatttgc tatttcaatc aataaaatcg ataacagtcc
     8401 cagaattaaa ggaaaagctt ttggaaaatc cagcgttact tgatgttaga acacctgcag
     8461 aataccgggg cgggcatata aaaggagcta aaaatgttcc attacaaagt ataaacagat
     8521 atgatggaga caaagaaaag accgtctatg tgatttgtca atcagggatg aggagtaaac
     8581 aagcagctaa agaattaaag aaatctggtt atgatgtagt gaatgtacgt ggtggcatga
     8641 atcaatggtt tgatcgaaca gtaggaggga aataagatga aaatcgttat tgtcggaggt
     8701 gtagcaggtg gaatgtctgc tgctacacgg cttcgccgat taatggaaga tgcagaaatc
     8761 gttgtttttg aaaaaggacc gtatgtttct tttgcaaatt gcggcttgcc ttattatctt
     8821 tcgggagaaa tcagtgaacg ggaaaatctt cttgtccaaa cgccagaatc attatccgct
     8881 cgtttttgtt tagatgtgcg tccaaatcat gaagtgacag ccatctttcc cgaaaacaaa
     8941 acggtagaag tcgtacatga gggtcaaaaa cacattgaac agtacgatgc attggtttta
     9001 tctcctggtg caaaaccagt tgttccatcg attccaggga taacagaagc cgacaatgtt
     9061 ttttctatta gaaatgtacc agatatcgat aaagtgatgc atgcattaga aaaacagcca
     9121 aagcgtgccg tgatcgttgg tgcaggattc atcggattgg aaatggcaga aaacctaaaa
     9181 agaagaggtt tagaagtcat ggtgatcgaa caagcaccac atattcttcc aacactggat
     9241 gaagaaatgg cagcttttat agaaaaagaa ttgtctcatc aaggagtaga agtgattact
     9301 tctcatgctg tcgctggatt tgaagaccac gggaaacgat tgcgactgga tgatgggcgt
     9361 accatccctg ctgatttagt tattttatcc attggtgttc gtcctgataa ccagctagca
     9421 gtgaccgctg gaatcgaatt aggcatacgc gggggtatcc tagtagacga acgttatcaa
     9481 acgaatattc ctgatattta tgcggtgggg gatgctatcg ttgtaaaaca gcaaatcact
     9541 ggaaaagatg cacttatttc tcttgcttca ccagccaatc gtcaaggtag acaagttgcg
     9601 gacacgattt ccggaatttc tcgaagaaat caaggcggta ttggaacagc aattatacga
     9661 acgtttggaa tgactgccgc atccaccggt ttaagtgaaa gaacagccaa agaaaacgaa
     9721 ctgtcttttg aagtcattca tgtatcagga aaagatcatg caagctatta tccagaagca
     9781 acagatattt tactgaagtt gatcttccat ccagagactg gcgagattta tggtgcacaa
     9841 ggtgttgggg caaaaggtgt ggataaacgg atcgatattt tagcaacagc aatcaaaggg
     9901 catttgacga tcttcgattt accggaatta gaattgacgt atgcaccgcc atttggctca
     9961 gccaaagatc cagtaaacat gctaggatat gcagcaatga acattgcaga agggcttagt
    10021 gaaaccgtac aatggcatga attgccgaca gaactagcaa aaggaaaaat tttattagat
    10081 gtgcgaacag cagaagaatt ggaaaagggc aaattcaagg aagccaaaca tatccctttg
    10141 aatgaacttc gggaccgatt aaatgaatta gacagccaac aagaatatat cgtcagctgt
    10201 catagcggat tacgtagcta tctagcggaa agaatcttga agcagtctgg ctaccacgta
    10261 aaaaaccttg atggtgcatt ttctttatat caaactgtcc gacctgaaga actgatatat
    10321 cctaacaaat gatactcgtt aaaaagcttg cattcgactc ttaaaagagg taaactaatc
    10381 ggttccacca ctttaaaaaa agaggagaat gtgtatggtc aacacacaaa aacagttaga
    10441 agaagcatat ctgaagaacg tgtatcaaaa gctaattgat aaaaaaaacg aactgcggga
    10501 atcgatggat acggcaaaaa tagaaggaaa ccagtcgttg aaagaaatgg ctggcgagct
    10561 tcgattgaat tttgacagtt acttagataa cctggatact ttttcgatga ttgaaatgaa
    10621 aaatagagaa atcgatcaga tgaatatcaa acagaaaaac gcagaaaatc agctagcaaa
    10681 gatcgaacgt ttattgaaaa gtccctactt tggaaaagtc gttgtagact ttctggataa
    10741 tgagccaaaa gaatcctttt atatcggtac agcaaatttc acagatgatg cagatgaaaa
    10801 tcttgtttat gattggcgtt caccaattgc cgaacttttt tacaataaca tgattggtcc
    10861 atcttcttat acggttcgtc aaaataaaat cgatacggtt attaaagaaa gacgacaatt
    10921 tcttttggaa agagatcgtt tggtcaagtt ttttgatacg tctgtagcta ttcaagatga
    10981 cgtattatta gaagtcttag gccaagatga gaccaatgaa atgaaggcta tcactgctac
    11041 gatccaatcg gaacaaaaca caatcattcg ggatgtgaaa agtcaaaata tcttggtaaa
    11101 tggcgtagca ggaagtggta aaacctctgc tatcatgcag cgtattgcgt atctgctcta
    11161 tttatatcga gatacgatga catccgatga cttgctgatt ctatcaccta atcatcggtt
    11221 tatcgactat atttcgaatg tcctaccatc attaggtgaa agaaatccac tgaatctaac
    11281 catcatacag ctggtttcac agctttcagc agaggaaata gaaggtgaag aggcttattt
    11341 caaacgtatc acgggagaaa atgtatcgga gcagacagaa cgtttgcgta gcaaaaagtt
    11401 tatcgataat ctaaaacaat cagatccttt gtttttagat catccgaatt ttatccgtgg
    11461 acttactaaa aatggaaaaa cagtcctttc taaaaaaacg atcgaaaaaa tatatgagaa
    11521 agttcctgct catcctaaat tgatcgatag gcttcaagca acgaaaaagg cactgatgag
    11581 cgaatggaag aatcatcttt tgaaacaagc aaaaagtcct gctgtgcaga atcaggtact
    11641 ctcgttgaca gaagataggc agttggaatt attcggtaaa ttgatttctg atgattcgga
    11701 acaaagtatt gtagcttatg caaggaaact tttacaaaaa aaatatcgaa aaataaccag
    11761 acaaatcgaa gaaatggcat gggtggatga gcatcagttg tttgaaagaa tttatgaaaa
    11821 acgatatggc tcagcttatg cttggcaacc cacacgtact gttgatgaag cagtcattat
    11881 attagctatt cgtcatttac ttgttgaaaa ggtaaatgta ccagcatttc gctatctatt
    11941 gattgatgaa gtacaagatt ataccctggc acaattaggt ctattgatcg aattatttcc
    12001 aaaaactcac tttactttag taggtgacga gaatcaggca atctttaatt cttccactac
    12061 ttttgctgat attatgcgtt gtttcgatga ctaccatctg ccgatccatc ggtatgatct
    12121 tagaaatagt taccgttcta gcggagcaat cacagagctt ttcaaaacct atgctgtgga
    12181 tcaagagaag atcgacatcg tatcgatccg accgcaagga aaagaacccg aataccgctc
    12241 ttttcgatcc tctgaagaat tgttgaaaat actagctgag attttggttt ctctaaaagg
    12301 agaaaaagcg gcagttatca cccaaacaga agaggaaacc caaacgctac aagaatcgat
    12361 taaaaaaaat tcatatggat tagaaaactg ccaaatcatt cctttgagtc tagcaaaagg
    12421 actggaattt gatcatgtaa tcttatatcc atttgaaaac gatggagatg agcaaagaag
    12481 acgcagacaa atgtacacgg cgatatctag aggaatgaaa agcattgtcg tattggaaag
    12541 agcaacataa ttaagagata aaaaataaaa agaacaactg ataaaaagaa caaaatattt
    12601 ttcatttgct gatgagaaat gttttgttct ttttcttagg aaaaatttag gaacacttta
    12661 tgtgaaaaga aatgaaatgt atggtaaact cctacgtcgt aacaaaaaaa cgtgaggtga
    12721 ggatatgaaa aaagtaacaa gagaagaagt tgcatcaatt ttggtgaaag ataagtattt
    12781 ttcaaaaggt aattactggt taaagatttg gcaaacgtta gtggctcttt taggatggat
    12841 gatcgttgtt cttcctttta tatgggtctt tttcccatta acgagaccag aaagagccaa
    12901 ggatttttcc ttgtacgctt ttttatcaga aaagaaaatg ctgtatttct taatcggctt
    12961 ttttgtattg atttttttca gtttcctgat tacctcattt gtattgacta ggtggaacaa
    13021 tcgaaatcta tacaaaaaag taaataaatc ttttgaatac gccgtttgta taattaagcg
    13081 agacaaataa aaaagccatt tatgttacac tc
//
LOCUS       DACGLH010000084        12670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.089, whole genome shotgun sequence.
ACCESSION   DACGLH010000084 DACGLH010000000
VERSION     DACGLH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.089"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            6..305
                     /locus_tag="IXT21_002386"
     CDS             6..305
                     /locus_tag="IXT21_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4869757.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IXT21_002387"
     CDS             378..1739
                     /locus_tag="IXT21_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ4869758.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IXT21_002388"
     CDS             1761..2021
                     /locus_tag="IXT21_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869759.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IXT21_002389"
     CDS             2035..2787
                     /locus_tag="IXT21_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ4869760.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IXT21_002390"
     CDS             complement(2881..3486)
                     /locus_tag="IXT21_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4869761.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IXT21_002391"
     CDS             complement(3539..4513)
                     /locus_tag="IXT21_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ4869762.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IXT21_002392"
     CDS             complement(4891..5670)
                     /locus_tag="IXT21_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4869763.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXT21_002393"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXT21_002393"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ4869764.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IXT21_002394"
     CDS             7161..8558
                     /locus_tag="IXT21_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4869765.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IXT21_002395"
     CDS             8639..9079
                     /locus_tag="IXT21_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4869766.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IXT21_002396"
     CDS             9227..9553
                     /locus_tag="IXT21_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4869767.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IXT21_002397"
     CDS             9554..10303
                     /locus_tag="IXT21_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869768.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IXT21_002398"
     CDS             complement(10383..10838)
                     /locus_tag="IXT21_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ4869769.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IXT21_002399"
     CDS             complement(11265..11540)
                     /locus_tag="IXT21_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869770.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEKGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IXT21_002400"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IXT21_002400"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ4869771.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2174 c         2588 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aacctttttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaggt
    12661 tgtgaaaaag
//
LOCUS       DACGLH010000085        12583 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.090, whole genome shotgun sequence.
ACCESSION   DACGLH010000085 DACGLH010000000
VERSION     DACGLH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.090"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            4..2376
                     /locus_tag="IXT21_002401"
     CDS             4..2376
                     /locus_tag="IXT21_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869772.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     VPTDFLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VREIEATPEDKKRIESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3906
                     /locus_tag="IXT21_002402"
     CDS             2437..3906
                     /locus_tag="IXT21_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ4869773.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3917..5926
                     /gene="topB"
                     /locus_tag="IXT21_002403"
     CDS             3917..5926
                     /gene="topB"
                     /locus_tag="IXT21_002403"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4869774.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6272..6868
                     /locus_tag="IXT21_002404"
     CDS             6272..6868
                     /locus_tag="IXT21_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4869775.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6881..7780
                     /locus_tag="IXT21_002405"
     CDS             6881..7780
                     /locus_tag="IXT21_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ4869776.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7783..7914
                     /gene="ssb"
                     /locus_tag="IXT21_002406"
     CDS             7783..7914
                     /gene="ssb"
                     /locus_tag="IXT21_002406"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ4869777.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7936..8268
                     /gene="ssb"
                     /locus_tag="IXT21_002407"
     CDS             7936..8268
                     /gene="ssb"
                     /locus_tag="IXT21_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ4869778.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8290..8547
                     /locus_tag="IXT21_002408"
     CDS             8290..8547
                     /locus_tag="IXT21_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869779.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8706..8828)
                     /locus_tag="IXT21_002409"
     CDS             complement(8706..8828)
                     /locus_tag="IXT21_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ4869780.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9685..9891
                     /locus_tag="IXT21_002410"
     CDS             9685..9891
                     /locus_tag="IXT21_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ4869781.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9891..10142
                     /locus_tag="IXT21_002411"
     CDS             9891..10142
                     /locus_tag="IXT21_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869782.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            10323..10595
                     /locus_tag="IXT21_002412"
     CDS             10323..10595
                     /locus_tag="IXT21_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869783.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11040..11219
                     /locus_tag="IXT21_002413"
     CDS             11040..11219
                     /locus_tag="IXT21_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869784.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11278..11634
                     /locus_tag="IXT21_002414"
     CDS             11278..11634
                     /locus_tag="IXT21_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869785.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         4640 a         2066 c         2617 g         3260 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgta
      661 ccaactgatt ttttgaagga gaaagaaaac gatcattttt atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcatactca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaatagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaaactta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gttggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 tcattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gctgaactaa agccgaaaca tgaacggcaa aattttttag ttggagacga ccgtttagat
     2941 catgcggttg cttttttatg gaaagatcca cagacaggag agacagtagg ggcaagttat
     3001 caaggaacga tcgtcgattt taatcgtttc ggtaaacggg gaacgtataa acacattgac
     3061 aagaatccta cgccaaatca cggatttaat ttaaaaatag gcgatccgaa gcatttaaaa
     3121 ttctttgaaa gtagtattga tctgttaagt tacgctgcat tgaatcggga gaaactacaa
     3181 gatgcctggt tagtttcgat ggacgggtta aaacatcatg taatcagtca ttacgttgaa
     3241 gagtcgattt ctgaattaag acgaaaacaa acatttcctc aatcgattga aatatgtgtg
     3301 gacaatgatc gtgcaggaca tattttttat gaaaaagaac agatgaaggg aatcgttgat
     3361 ccttttacca ataagaaaat ccgttgtgag cgaggaattc caaatgattg gcaagtacct
     3421 aaagaataca aagctactta tgaagcagtc gcaaaagaaa tgagtgttga acctgaagca
     3481 attatggcca tacacaaaac tgaaactaat ttacagctga caaatcaatt ggtgtctgct
     3541 catgatgttc aatctacgtt tggaaagatg ttggcgaagg gcgaaccagt agaaacaatt
     3601 gatttgaaag aagcatgtac cacggtcgcg aaggaactga aagtatgtga acgcgcagat
     3661 ggaacataca actttgatcg tttttatagt agaaaagcca acataaaaga cgttaatgca
     3721 ggaatccttc tttcctataa agcagaacaa tactacaaag gctataaaaa gcatgaacat
     3781 gaatttgtac ctgaagtgaa aaaggattgg aatgatcaat tgaagcatga gatccagcaa
     3841 caagaaatca gaaagcaaaa acgtgcgatg ttgtttcaac aaggcagaca acaagaaagg
     3901 gagtgaagtc gtcactatga aaaccgtcat tttagcagaa aaaccgtcac aagcgaaggc
     3961 ttatgccgat agcttttcta aagctacccg aaaagatggg tattttgaga tccaagatcg
     4021 attgtttaca ggagaaactg tgatcacgta tggttttggt catttagtcg agttagattc
     4081 gcccgatatg tacgatgaaa aatggaaaca atgggcatta gaacatttgc ctattttccc
     4141 caatcaatac cactatcatg ttcctaaaga taagaaaaag cagtttagtg tcgtcaaacg
     4201 acagctgcag tcagctgata cgattgtgat cgcgactgat agcgatcgtg aaggggagct
     4261 gattgcctgg tcaatcattc aacaagcagg cgccaatcaa ggaaaaacct ttaaaagact
     4321 ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt tacgagacgc
     4381 agaagagacc tatccaaaat ttgaagaagt acaagcacgt cagatcgccg attggttaat
     4441 tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     4501 tttttcttta ggtagagtcc aaaccccaac actctatatg atttatcaat tgcaggaaaa
     4561 gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gcccaagtaa tcgctcaaaa
     4621 tggcgcattt gatgcgaagc ttgatccaaa tgaaacccaa gcgacacaag aagcatttga
     4681 agcctattta aaagaaaaag gtgttcaatt aggaaaacag ccaggtacga tccttcaagt
     4741 cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga gccttcagtc
     4801 taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca tgcaagggtt
     4861 gtatgaagga aaatatctaa gctatccgcg aacggatacc ccttatatta cggaagggga
     4921 atatgcgtat ctgttggatc acttagacga atacaagcat tttttaaaag ccgaagcgat
     4981 tccgactcct attcatacac cgaatagtcg gtatgtgaat aataagaaag tccaggaaca
     5041 ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac aactaagtcc
     5101 actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     5161 gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagt taaaggcaat
     5221 cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga gcgacggaaa
     5281 agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg tgacggtgga
     5341 tctacaagtt cttgaaaaag aaaccaagcc accacaacct tataccgaag gtacgttgat
     5401 cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat ccatattgaa
     5461 agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca
     5521 aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag ggattttgct
     5581 ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg ccaaatggga
     5641 aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc tagccaatat
     5701 ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat ccactgattt
     5761 tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt
     5821 aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg tggcagcttg
     5881 tacgtctgaa gcctgtgatt ttaaaaggtc agaagggttc atttgaagca acgattgttt
     5941 taaaagaaga cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga
     6001 aaagaacacg ccgcactgca aaataatgga gggttaatct tatgcgtaat accacacaca
     6061 aacaacaaga aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg
     6121 ccagcaaacg aagactgcag caattgaaga accagcagag gattcaaaaa gaaatcgaat
     6181 tagatcgata aaatcttcct aactattgta tacgtataaa cttatgttat actgttttta
     6241 taagtttata cgtatacaaa ggagggagaa aatgacaaaa gcaatggtat acaagctgct
     6301 agaagaattt aatggaactt tggctttgaa ggatgccaaa aaagcgggca tttctcctgt
     6361 aacgatcaaa cgtatggtag atcgagggga gctggatcga gagtatccag gttttttcac
     6421 cttacctggt caatttcctg atgaattgtt tatggcgcaa aaaaaatatg aacgcggcat
     6481 tatttcacat attaccgcgt tagatttgta tgatttaaca gatatgatcc ctagacagat
     6541 cgatctgact gtaccttacg gttatcacgt ttctgaaaaa ggattgaagg aattcgcagt
     6601 tgagctacac tatacaaaag cggaatggta tgaattaggt aagattgaaa taaaaagtcg
     6661 ctatgggaat cctattatag cttatgatcc agaaagaact ttatgtgaca tttggaatcc
     6721 ctggtatacc gttgaggatg aaatcaaagt aaaagcaatc aaaaactata tggagtcgga
     6781 cagaaaaaat cttagaaaat tgaatgagta tcgacgaatt ttaccaacag acaaaacgat
     6841 gcgttcttat attatggctt taaattaaag gagaagatac atgacaccta cgcagttaaa
     6901 agcaaaagtg aagaatattt ctagagaaaa agaagttgat ccgcagcttg ttttaagaca
     6961 ttttatgatg gaaaaattct tggaaaaaat ctcagagtct ccttatcgtg aaaattttgt
     7021 gttaaaaggc ggcttcttga ttggctcaaa gtatggcatt gaaaatcgca caaccaaaga
     7081 tattgatacg actttacggg aaatgaaagt aaccaaagaa acattaacaa cggttctaaa
     7141 cgaaattttt tcaacgccga cgaaagaagg cattcaattt gagattcaag gaatgaaaga
     7201 aacgagagaa gccgattact atccaggatt tagcttacgt gtgctggcac atttggaaaa
     7261 catgcggcca gattttaagg tagatgtgac tacaggtgac tcgatctatc ctgcaacgat
     7321 cactcacagt cataaattga tgtttgagga tcgaacgatt cctttggaat cctatcctac
     7381 agaacaaatt attgctgaaa aattaagtgc aacctttgat tttttaacag ataattcgag
     7441 aggaaaagat ttttatgatc tctatactat tcctaaaatg gagaagatcg atgagaagac
     7501 cttgaaagat agtgtgagaa atacatttac acgtcgagga aaaactgatc cgttaaagat
     7561 atactatgaa aaggatatgg atgtactgaa aaatagtcac gagctaaaag atacctggaa
     7621 gaaatatcaa aaaagcaatc cttatgctgc gtcgatcacg tacgatgaga caatggattc
     7681 gattagtaat ttgatggaaa aagtgattca cgtcgaagaa aaagagcgaa aactagctta
     7741 tcaaagacac caacagatga atagagaaat ggagagataa agatgattaa taatgtgaca
     7801 ttagtaggac gcttaaccaa agatcctgat ttacgttata ccgcaagtgg tactgcagta
     7861 gcgactttta ccctagccgt caaccgaaat ttcacgaatc aaaacggcaa ttgagaagcc
     7921 gattttatta actgcgtgat ttggcgcaaa ccagcagaaa caatggcgac attagctaaa
     7981 aaaggtagct tgattggtgt agttgggcgg attcaaacgc ggacttacga caaccagcaa
     8041 ggacaacgtg tatatgtgac tgaagttgta gcagataact ttcaactatt agaaagtaaa
     8101 gcagcgacag agagccgtgc gcacgctgat caatcgtcca ccagtccaag caccgccacc
     8161 tttgaacaac ggaatacagc gacgcctaac aacaatggat tgaacgcttc acagaatcca
     8221 tttggtggac aatcaatcga tattagcgat gatgatctac cgttttagtt tgagaaagaa
     8281 ggagtaagca tggcaaaaaa aattagtctg acgatggatc tattgatacc cgtaattgca
     8341 tttatagacg ggatttcttt gggcgtcact gaaaatcctt tggtaaatag aattgcgaaa
     8401 ggatcactat ttcttgtatt cgtgtatctt gtctgttctt atcttttaaa aagagcagga
     8461 aaggataatt ccttttggcg attgactaaa gtcacagtag cagcgacagc tgttatactt
     8521 acggctattc taccgcttta tatgtagagt taacgggaaa tcgttgaact tcttgcgaaa
     8581 tattgctata atgaacatga aaaagaggag ttgcgctaac aacccctcat taacagcacc
     8641 gattaaagaa cggtggcaga atattagtta tttggaaata accgctgacc tgctaaagta
     8701 gacggttatt tctttttgtc atttttaagc atatttacaa tcaaactaat caaagcaatg
     8761 gtaaacatac caaaaccaag aatggtttga attgtctcat atgcggacaa aaggcttact
     8821 cctttcatag attttagcac ttacattcat aagcaccacc acctttccag aagtcgccac
     8881 cgtcttcaaa ctttactgtc ttgactatta tagcatatct aaaatcgaat aaaaattctc
     8941 aattttagct gtttttgcta taattttagc gaggtgatcg tgatggaaca atatattggt
     9001 ttactcgata aattagggtg atcaagacac agccattgct tgtgcgattt acattacgca
     9061 cgagtcatga accaatcaat tgtgtagtgg ccaatataga aatagcggat aaattgctga
     9121 ttttggaaga aggcaagtat actatttcag tgacaggtca cttcaataaa cgaaagcaac
     9181 tagtcattga atcaatgcaa attagaaacc ctgatcattt tacgcgatcg atgagcatat
     9241 aaaagaagag ctgcagtcct aaatgaggat tgtagctctt tttgttttac atagaaacag
     9301 taaaaatgaa aaggcctgtt taggaaggca caagcgcctt ctcaattgaa agacctttct
     9361 ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctacg cgttcactaa
     9421 gataaagtgt cttgcatagc aagacaaaat agaaaaaatt tcctctcacc aaaaggttcg
     9481 ctcctgttcg ttatctcttt attgatttgt cagaccggca acaagttgcc ttttatcacc
     9541 aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcttg tcgcttttcg
     9601 ttgattcatt ctggccaaca aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt
     9661 tcctaagagg aggcagaatc aatcatgcga aagaaatttg attattgggg tgttcccttc
     9721 agcgaattat tcccaaacta tcatgcacca catactgtcg aatgcgattg tggagaaaga
     9781 gcgaaatgta tcaaaagcta ttgtttgtat caatgtccaa catgcgggaa aaaatatacc
     9841 ttgtcttatg gcgattatat tttaattgat gagaaaggaa agaaacgctg atgaaaacac
     9901 aacacaaatt attgactgta aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt
     9961 ctgaaaaagt agcagaagtt ttacttattc cgcggtactg gacgatagaa acccctttag
    10021 tagaggatgc accgactatg ctatttgaag acctagtgaa gattgcagga tatgaactgc
    10081 attatcaaga aaacttggaa acttatgaaa acgaatattg gttaaccaaa ttagagaact
    10141 aaaaaggaga aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg
    10201 ataaaatggc acaaaaaatg gaggatatga cgttcctata caacgaaaca cgtgttccaa
    10261 aaaaacatta taaagaaaaa ttgtcggtag ctgtggaaga aatgattgaa taaggtgtcg
    10321 aaatgaatct tattgcgacg tactatcgaa cactggaaga actgaaaaag caaaatgcaa
    10381 aatggttttt ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actattaaac
    10441 cttctgaata ccaagcttta gaactaacgt atgcgaaatt tatcgaaacg aaaaaagcca
    10501 agactgtttc aagtgaatgg ttagattatt ttgaaaatat caataagtat ggcgcatatt
    10561 acaccatgaa aaaggaagac aacgaaaatg aataaggtga tttatcaact aacaacggat
    10621 tccatatcaa cgatttgaaa gaaaaaaagg tcaataatca tttataccga aaagtgagaa
    10681 tgtgtgaaac aaggacaaaa aaagcctagt aactaaggaa atggttctta tttcgcacca
    10741 atcgctccaa ccgcccccgc cgcttaggtg gtgcgaaata aacgggcaaa gcccgataac
    10801 ttaagagaaa gaaggtgatc catttgaaca agattattcc cttattgtta gtactgtttg
    10861 ttggcgaaat acttatgcga gatagtgcag ctgtacagtt aaacctcgct gtatttttgg
    10921 taatactggt gttttgtttg atcttttggc ggtttgtatt agtcctcgtg agtctactat
    10981 tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag tgagtaccaa
    11041 tgccaattgc aataggaaat aaacggttac ccgtgacatt agatgaaaag agacaaaaag
    11101 aattgcagca actaaagcag aagtatggca aaagtgaatc taagattatg tgtattgcgt
    11161 tagatctatt gatcgcgcaa gaaaaagcag gatttgatgt accagcgcta aaaaagtgac
    11221 gtcacttttt aatttgatct atataagtga tatcactttt accaaggagg gaattaaatg
    11281 ttaaaaacta aaaagaattc gtacaaatta aagcttagta tactatctat tatttgtctt
    11341 ttaggcatga taattagtgg tcatttgtat agagtggcat atacgcagtt tattgagtac
    11401 aaggttcact ctttaggatt agctggggat cgatacgttg aatatgtcca ttattcatta
    11461 aaatgttctt catatattct tctttggctt attttttcat tgtttgaatt gctaaattta
    11521 atagcaatat cttcatatca cttagataag ctaaatacca aatctagccg ttataacgaa
    11581 gtgtttaagg aaaatgtgaa gttagaaagt gacttaaaca agctaaaaaa ataaaaagaa
    11641 gaaaacataa gttaactaaa attattttat taagaatgat ggaatataag gataaggtta
    11701 ccaacataac gatcattact tctctgatat gctatggact tctatcaatc agtattttgt
    11761 ctatagatta tggagtaact atactgcaag ccttagtttt ggctatgcct atgtatctat
    11821 ctttgtttat catgcatgaa atatataagc tatttgtttt atcaaaaaat gtagaaatac
    11881 gggtaaatat tttggtgaat agttatcaaa aagaactcaa agaaagaaga cggtggcggt
    11941 atttattgaa aggatgaaaa agtaggattt gacatactgg tattcagaaa gtgccgtcac
    12001 ttttaaaaca atggaaaagg tggagataag atgaagaaaa aacaaactaa actatcatta
    12061 gcagatattt aacacagtta accgcaactg aagatggtgt tgttgaattt gaacaatctg
    12121 aaattgcggt agtagatgat cgttacctta aaatgcctta cttctttgac caagcaaagg
    12181 tcatttgcct ttttcactac gatggggtac gtgattttag gcgttaaaaa gtagtgatta
    12241 tatcaacttt acgagtaaat ataaatttag acatgaaact gaattatcca atggaaaatc
    12301 atggtttata atctagaaaa attatagtaa tgattggatt atatatattc tttcgtattt
    12361 tcgtacaact gtagcttgtc aaaattgaga aatttaaccg attaggtact ttacatcgtt
    12421 atttgggtaa tttctaaaac atgaagctta ccaacgaata aactgagact atcttattaa
    12481 ttgatcaata taagaaaata tctttgagga gatgatgtac tatgttttcg tctatccacg
    12541 attggttagg tggtcttttc ggcggtggcg gtaaaggttt tta
//
LOCUS       DACGLH010000086        12226 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.091, whole genome shotgun sequence.
ACCESSION   DACGLH010000086 DACGLH010000000
VERSION     DACGLH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.091"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..566
                     /locus_tag="IXT21_002415"
     CDS             <1..566
                     /locus_tag="IXT21_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ4869786.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /locus_tag="IXT21_002416"
     CDS             967..1572
                     /locus_tag="IXT21_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ4869787.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="IXT21_002417"
     CDS             1588..2160
                     /locus_tag="IXT21_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4869788.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="IXT21_002418"
     CDS             complement(2412..2669)
                     /locus_tag="IXT21_002418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869789.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="IXT21_002419"
     CDS             complement(2795..3610)
                     /locus_tag="IXT21_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869790.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="IXT21_002420"
     CDS             4127..5452
                     /locus_tag="IXT21_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ4869791.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="IXT21_002421"
     CDS             5445..5795
                     /locus_tag="IXT21_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869792.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(6866..7141)
                     /locus_tag="IXT21_002422"
     CDS             complement(6866..7141)
                     /locus_tag="IXT21_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ4869793.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="IXT21_002423"
     CDS             complement(7113..8066)
                     /locus_tag="IXT21_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4869794.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="IXT21_002424"
     CDS             8678..10171
                     /locus_tag="IXT21_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ4869795.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="IXT21_002425"
     CDS             10306..10602
                     /locus_tag="IXT21_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ4869796.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="IXT21_002426"
     CDS             10703..11554
                     /locus_tag="IXT21_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869797.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="IXT21_002427"
     CDS             11575..11787
                     /locus_tag="IXT21_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869798.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4205 a         1873 c         2286 g         3862 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca cctggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaacggttc tgttgcaaag ttttaaatct actatcaaat
    12181 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatc
//
LOCUS       DACGLH010000087        12202 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.092, whole genome shotgun sequence.
ACCESSION   DACGLH010000087 DACGLH010000000
VERSION     DACGLH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.092"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            506..1069
                     /locus_tag="IXT21_002428"
     CDS             506..1069
                     /locus_tag="IXT21_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869799.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1130..1495)
                     /locus_tag="IXT21_002429"
     CDS             complement(1130..1495)
                     /locus_tag="IXT21_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869800.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1571..1816)
                     /locus_tag="IXT21_002430"
     CDS             complement(1571..1816)
                     /locus_tag="IXT21_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4869801.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1886..2140)
                     /locus_tag="IXT21_002431"
     CDS             complement(1886..2140)
                     /locus_tag="IXT21_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869802.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2304..2627)
                     /locus_tag="IXT21_002432"
     CDS             complement(2304..2627)
                     /locus_tag="IXT21_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869803.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2814..2887
                     /locus_tag="IXT21_002433"
     tRNA            2814..2887
                     /locus_tag="IXT21_002433"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2848..2850,aa:Ile,seq:cat)
     gene            complement(3272..3769)
                     /locus_tag="IXT21_002434"
     CDS             complement(3272..3769)
                     /locus_tag="IXT21_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4869804.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3809..4618)
                     /locus_tag="IXT21_002435"
     CDS             complement(3809..4618)
                     /locus_tag="IXT21_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4869805.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4952..5809
                     /locus_tag="IXT21_002436"
     CDS             4952..5809
                     /locus_tag="IXT21_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ4869806.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5802..6704
                     /locus_tag="IXT21_002437"
     CDS             5802..6704
                     /locus_tag="IXT21_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ4869807.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6737..7087
                     /locus_tag="IXT21_002438"
     CDS             6737..7087
                     /locus_tag="IXT21_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ4869808.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7121..8059
                     /locus_tag="IXT21_002439"
     CDS             7121..8059
                     /locus_tag="IXT21_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ4869809.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8137..9030
                     /locus_tag="IXT21_002440"
     CDS             8137..9030
                     /locus_tag="IXT21_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4869810.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9068..9631
                     /gene="amaP"
                     /locus_tag="IXT21_002441"
     CDS             9068..9631
                     /gene="amaP"
                     /locus_tag="IXT21_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ4869811.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9645..9833
                     /locus_tag="IXT21_002442"
     CDS             9645..9833
                     /locus_tag="IXT21_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ4869812.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9846..10748
                     /locus_tag="IXT21_002443"
     CDS             9846..10748
                     /locus_tag="IXT21_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4869813.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10780..11022
                     /locus_tag="IXT21_002444"
     CDS             10780..11022
                     /locus_tag="IXT21_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4869814.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11278..11649)
                     /locus_tag="IXT21_002445"
     CDS             complement(11278..11649)
                     /locus_tag="IXT21_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869815.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            11855..>12202
                     /locus_tag="IXT21_002446"
     CDS             11855..>12202
                     /locus_tag="IXT21_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4869816.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKN"
BASE COUNT         4297 a         2065 c         2313 g         3527 t
ORIGIN      
        1 attggtcgtt gaatgggctg aatagtagtg tataaaacaa ttttgtggag cgtgaagctt
       61 ttatggtaga ggaaaatacg aataaagtga tgttgataaa aagattccta tcttgaatat
      121 gaatcgagat gtcttgaaaa tgaatgagtt tgaccaatca ttatcctatc gaaatttaca
      181 gtttcgttcg attggtctgt caattaatga aaaagtaaaa aagaatatgt tctaggaaga
      241 gggcggcaca tctaaatttt taagtaacgg acagttcaaa tatcaagcat tgaaagaggc
      301 tatcctactg tttgaaggaa tacgaatata taaaaacgac aaagaggaaa aagtagttca
      361 ttaaatccct tcaagtgatg aaagaatgag atctaataaa acctgatctt tcatctgttt
      421 ttttaataac gtaataattt ttttattttt tgtgctttcg ccgttattgt agagtgattt
      481 agcattctta tttttaaaaa taactgtgtt atatattaaa aaaggcataa tagttataag
      541 cttttttaaa gaatatttag aaattattaa ttttcgtctg aaaacaaaaa aagaaagcgt
      601 tattatttta gtctattttc agggggggaa ccttatgata aaaaaactat ttttactatc
      661 aatattaacg ctagcaatgg caggttgttc tgactctggc ttagcaactg aactgatcac
      721 aaaatcaact gaagccacga cagaggagtt aaaatgtgga aacgatcaca gtgactacag
      781 aggcttaaat cgacgatgca tgggaatatt aggcagaagc aattgtaagc atcgatgtag
      841 taatcgaaga aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa
      901 tctgtgctgt gcatcgtata cagacgtcga agatgcggta cttttaaacc acgtcaggag
      961 atgttcattt atgtctaggg aagttggcga tgaaccaaaa ctagagtacg cttttagttt
     1021 tgcacgtgag gatgaagata accctaaaac ttacgacatt ttagcttgat ctaagatggt
     1081 taaagctttt taatgaagta agaggagata gaaggctttt ctagttatat taggtaagat
     1141 aaattccttt ttgctccaaa tttgctaaac attctgctag ataaacttca tcgtgttccg
     1201 gataaatcgg cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg catgatttgc
     1261 cacgatacat aggatttttc cactctggtg ctgggctgta accacgtttt tccatttcat
     1321 ccatgacaag ctgatgatat tggaataatc tataaggaga atagtgaaaa acataatcca
     1381 cagtagcatg tttctttccc cagccatttc ctcttaatgc acagcattcc cgatgttgtc
     1441 ctaaaagctg ttgacgagga agctttgaaa tcaatttttc atgccataat cgcatagaaa
     1501 tgtctccttt atatatagat tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg
     1561 ctggaaaaaa tcacttcagt ttgcttgctg tttctgagcg accaattagg taatcaacgg
     1621 aaatatcgta aaagtcagcg atatagatta attcaatgat gcttggaatt tcttctcctt
     1681 cttcgtactt acgatatttg aataaaggaa tacttaacgc gctagaaaca gctacttgag
     1741 ataaagatcg gccagttctt aagaaattca gacgacaacg taaagtatcc agtaaaatag
     1801 cttcttcgat actcatttaa tcacctttca ttatagtaga atagttataa tgactataga
     1861 aagctacaca tgcaatcaat agtactcaat gttttttatt taaacgtatc aaataaagaa
     1921 tgacgatcaa taaagacaaa accagtcctg ataattcttc tataaaaaca ctatcgggtt
     1981 caaagtagtt gtaattaaaa actttccaca taaagtagat agccggaaga gtcaaaatag
     2041 ctaaataaaa ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga
     2101 ccgcaggcac ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat
     2161 tcatataata aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa
     2221 aagaagaata gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa
     2281 ctgttccgct gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa
     2341 ttcaatacat ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca
     2401 tagcgaatgg aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct
     2461 ttatagctga gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca
     2521 ataaacagaa ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact
     2581 aaatcgaata aaatcgcaat aaatggcaaa ataatcactc ctaacattga gaataaataa
     2641 aagaataaca ttttttctaa aataagcaaa taaaaattaa agtaacaaaa tagttatgac
     2701 acaagtaaaa gaaataagaa taacaatatt attttactta tattttgaaa cttgtgagag
     2761 tttgtcattc gtgctatagt tgaaaagtaa caaaaataaa ttttatttat gatgtacctt
     2821 tagctcagtt ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca
     2881 aggtacataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
     2941 aaacagcttc ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat
     3001 accgtttaag gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg
     3061 gaaaaatagt tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga
     3121 acaccattta agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa
     3181 tctactttga tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg
     3241 tgttaaatat cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat
     3301 ctttcaacag ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa
     3361 caaaaaagtc tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca
     3421 agtttgtttc aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag
     3481 tttcctggat ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc
     3541 gttcattttc ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt
     3601 tgtccttcag ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg
     3661 ctttagctaa tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt
     3721 cgctgtttgg atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc
     3781 ctacattcat atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac
     3841 cagcttcttc attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat
     3901 tccccatcgc aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc
     3961 caaaagccat gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat
     4021 gagctttagt gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt
     4081 ggataccatc tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg
     4141 ttaatgctaa tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa
     4201 aagaatggtt ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt
     4261 catcgaatgt cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt
     4321 cttgagcata gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa
     4381 ctttcgccat ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat
     4441 agccaccgat tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag
     4501 cgacggttac gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg
     4561 gtatctttcc atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt
     4621 tatcttccta ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat
     4681 ttaaaaaaat gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag
     4741 aaaaaggtgg ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa
     4801 acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga
     4861 aaacaaagca tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt
     4921 tagaaaaaga ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt
     4981 ggtaactgct tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt
     5041 agaaaattgc aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga
     5101 atatagtaaa gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca
     5161 attcctgaca gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac
     5221 taccgcttac ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt
     5281 cctttactta gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc
     5341 gttaaatgga aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag
     5401 attaggaatc gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga
     5461 tcaatggaat aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga
     5521 tcgaatcatc tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca
     5581 acttcatcca aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa
     5641 tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac
     5701 aggacatgat aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa
     5761 aaaattagca cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag
     5821 cagtagtcgc aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag
     5881 tattagataa ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg
     5941 attttgaaag taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg
     6001 tggaattctt gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc
     6061 cagaaacagc ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac
     6121 gtatcttata tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc
     6181 ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg
     6241 aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca
     6301 atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata
     6361 atccagaaaa attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt
     6421 ggttaccact tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac
     6481 atccaacagc agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc
     6541 atttttcagg acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt
     6601 acgaaaaata tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa
     6661 aatatgcaaa agttcaacca aaaacagatg tagttgttaa ataatagaag acaacaactg
     6721 aagaggggga gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga
     6781 aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct
     6841 aagggacaaa ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga
     6901 atttggagaa tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt
     6961 agagcaaaat tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt
     7021 ttcattagaa aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt
     7081 cagctaatag tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga
     7141 tgaagaaaag aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag
     7201 tgcaaagccc gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt
     7261 cgtaggaacc gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact
     7321 agagcatcca ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa
     7381 aaaattaacg ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag
     7441 aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga
     7501 aaagatcatc acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga
     7561 gatgaaaaag cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga
     7621 actgtataaa caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga
     7681 tcaacgggaa acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga
     7741 tgaacagcta ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat
     7801 ctatcgagat ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct
     7861 aaatcatatc atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt
     7921 cgcagggatt tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt
     7981 tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg
     8041 gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt
     8101 tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac
     8161 ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta
     8221 cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa
     8281 ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg
     8341 attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa
     8401 gatgcagagg aagtagcaaa atacgtgaaa gttgcaggac gtaagattgt cttactacct
     8461 tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt
     8521 ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct
     8581 gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct
     8641 ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct
     8701 gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc
     8761 gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt
     8821 gtagcacctg gtccaatctg gacaccatta caactagacg aaggacaacc ttctgattcg
     8881 atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca
     8941 ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga
     9001 gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg
     9061 tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc
     9121 tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa
     9181 gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt
     9241 tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat
     9301 cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga
     9361 aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt
     9421 caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc
     9481 actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt
     9541 gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa
     9601 acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt
     9661 cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga
     9721 tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta
     9781 tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa
     9841 catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt
     9901 ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca
     9961 aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag
    10021 aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta
    10081 aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc
    10141 cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa
    10201 aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa
    10261 tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg
    10321 aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac
    10381 ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag
    10441 cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa
    10501 tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca
    10561 acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc
    10621 aagacaaagt aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata
    10681 aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg
    10741 ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt
    10801 atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg
    10861 gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta
    10921 ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc
    10981 attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag
    11041 gtcgtgaaaa agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca
    11101 tatttttcat ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg
    11161 gaataagagg ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca
    11221 attagatttt taaaagtttt tccttaaatg tgtctttatt gatacagaga agcttcttta
    11281 ataaaacaat gcctccctaa attttcttga gataggaaag gtgaacatac aaaataatac
    11341 gaccactttg aaaacccaga tgatccacaa atggatcgta tgatactgat gaagtaaaaa
    11401 gcaatggatc agaaactcaa agagattgaa aaagacaagg aagaaataat atctcttttt
    11461 tactcttgtc tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag
    11521 taaacaaagt ttcgtaagag cagcaatcgt atcatgttgc tctgctagac gattgacgag
    11581 caggaaaaat gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat
    11641 aaatggcata ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt
    11701 tgtttgtgaa gctttttcgt gtcgtaacaa atgttatcta atgtattttt aaataaataa
    11761 tcattattat caaaataatg tcagactgta gacaaagtcc attttatgga tgatgtctac
    11821 agtcttttct tttataatag atttatgaaa acgaatgcaa ggagtgatag ctatgatgtc
    11881 taaaaacaat acgaatggta gaaatcaatt tgcgatgctt acgattgatg atctagttcc
    11941 tcaagatcat ctcgttcgaa agattgatgc cgcgcttgat tttgaattta tttatccaat
    12001 cgtcgaagcc acttactccg atttaggtcg cccaagtatc gatccagtga ttctgatcaa
    12061 attggtgttt attcagtatt tattcggtat ccgttccatg cgacaaacca ttaaggaagt
    12121 cgataccaat gtcgcttacc gctggttttt aggctactcc ttcgaggaga aaatccccca
    12181 tttctcaact tttggaaaga at
//
LOCUS       DACGLH010000088        12099 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.093, whole genome shotgun sequence.
ACCESSION   DACGLH010000088 DACGLH010000000
VERSION     DACGLH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.093"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            347..955
                     /locus_tag="IXT21_002447"
     CDS             347..955
                     /locus_tag="IXT21_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4869817.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            1123..2760
                     /locus_tag="IXT21_002448"
     CDS             1123..2760
                     /locus_tag="IXT21_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ4869818.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2832..3944)
                     /locus_tag="IXT21_002449"
     CDS             complement(2832..3944)
                     /locus_tag="IXT21_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ4869819.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(4019..5392)
                     /locus_tag="IXT21_002450"
     CDS             complement(4019..5392)
                     /locus_tag="IXT21_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4869820.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5613..6230
                     /locus_tag="IXT21_002451"
     CDS             5613..6230
                     /locus_tag="IXT21_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869821.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6328..6783
                     /gene="argR"
                     /locus_tag="IXT21_002452"
     CDS             6328..6783
                     /gene="argR"
                     /locus_tag="IXT21_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ4869822.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6801..8303
                     /locus_tag="IXT21_002453"
     CDS             6801..8303
                     /locus_tag="IXT21_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ4869823.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            8398..8604
                     /locus_tag="IXT21_002454"
     CDS             8398..8604
                     /locus_tag="IXT21_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869824.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            8690..9091
                     /locus_tag="IXT21_002455"
     CDS             8690..9091
                     /locus_tag="IXT21_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ4869825.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            9246..10577
                     /locus_tag="IXT21_002456"
     CDS             9246..10577
                     /locus_tag="IXT21_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ4869826.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            10599..11225
                     /locus_tag="IXT21_002457"
     CDS             10599..11225
                     /locus_tag="IXT21_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4869827.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            11408..11989
                     /locus_tag="IXT21_002458"
     CDS             11408..11989
                     /locus_tag="IXT21_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4869828.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
BASE COUNT         4173 a         2160 c         2526 g         3240 t
ORIGIN      
        1 ttgttgtaac aattactggt ttttttgtat tttttacatt tttttaagag aatttgataa
       61 aaatcaaaaa tttttttgct tttttttatg gattattgca gtgaatgtta cagaatctgc
      121 gtttttcatt caaaaataaa aaaaacatag aaaaacgatt actttgctta tacaacaagg
      181 cttttatggt tttgtatcaa aaaaatattt gtttaaatgt tacaaatatt aaataattcg
      241 ttattttgtc attaaatgtc atgtttcttt tacctatgaa atatttctaa atgataaagt
      301 taatagcgtc gaaagaataa aaaacggaga aaaaaaggag tttaatatga attcatttaa
      361 gaaaattgtt ttaggtacaa cttttgctgc tggtgctact gcaatgtttg taggaactac
      421 aaatgctcac gctgatgaag tttatacagt aaaatcaggt gattcattat ctaagatctc
      481 acaaaaattt gcaggagaca actcaatgat cgatgcaatt gctgaaaaaa attcgatcgc
      541 aaacatcaac cgcatttatg ttggtgaaca attaacaatc ccaacttcaa atgattcttc
      601 agcaacaaca gaaaacaaaa caacagaaaa tacagcatct acaactgaaa cagcaacgca
      661 agaacataca tacgttgcac ctgttgaaac agttgaagta gctcctgctg ccccagctgc
      721 tgcaacagcg ccaacttcaa gctctgcaaa agaatggatc gcacaaaaag aatcaggcgg
      781 ttcatacaca gctactaacg gacgttacat cggccgttat caattagatg cttcatactt
      841 aaacggcgac tattcagctg ccaatcaaga acgtgtagca gaacaatatg taacatctcg
      901 ttatggttca tgggatgctg ctaaaacatt ctggttagca aacggctggt actaagccga
      961 taaaaggaaa atgatgaaac cgagaaactt gtttattcaa gttctcggtt tttttgtgtg
     1021 aaaaataact cgtttgcttc ccacagcgca gcagtacaaa taaaaactcg aataatgtat
     1081 aataaagaaa acgttttttt gtttagggaa tgaggaggag ccatgagtac aaaagagagt
     1141 ctcgaattaa acgaaatcac aagtggaaat ccactgttat ccagtattcg atcgattgta
     1201 gaaacgacat tttacggtaa taatgttcat gaagtttttg ataggaagac agcttatcaa
     1261 ctagcaaaag ggagtccagg aacgatcatc acagatttaa caatctcaca tgctgaagaa
     1321 ctagatttac cagctgatgt taggacacta gtattcaatg atggaagcat tgtgggaaga
     1381 acagcaagtg ctcggcggat ctttgaggac ttagacaagg aacaaagcaa atacgagaaa
     1441 attttaagag aagctgtgta tcaaagtcgc aagcgccagt tttaccatac aaaagtaatc
     1501 gttggcctat ctgaagaatt ttctgtgcaa agtcatttgc tgattccaga aggatttgag
     1561 aacaatttgt attcttattt gctgaatttt cagccagcga attcgaagat cttagagact
     1621 tatcaaaagt ctgtttcctt tgatgaggga gatctttata tcttttgtga tcccgaatgg
     1681 acacatgaaa catttccaaa tggactcatt ttgtttgatg ctctccataa tacagcggtt
     1741 gtgctaggat tacgttactt tggagaatta aaaaaagcga ctttgacgct tgcttgggca
     1801 ttgggaaatc gcaatggctt tattgcctgc cacggtggga tgaaacaata tgctagtaat
     1861 gaccagacct atacgatggc tgtatttggg ttgtccggtt ctggaaaatc aacgattacc
     1921 ttagcaaaac atacggaagg tcactctgtt tcgatcttgc atgacgatgc tttcgttatt
     1981 tctcgaaaaa atggttttgc tactgccttg gaaccttctt actttgataa aacacaagac
     2041 tatccgtctg atgatccggc catttcttct ttccttactt gtcagaatgt gggcgtgaca
     2101 tgtacaagta aaggagaaaa agtcctagta acaggagata tacgaaacgg aaatgggcgg
     2161 acagtcaaat cacgttatgc gacacccgac cgagtcgatc atcttagcga aaagatcgat
     2221 gctgtttttt ggatcatgaa agatgattcg cttccgccaa ttatcaagat caatgaccca
     2281 gttttagcag cgtcttttgg tgcaacatta gcaacaaaac gatcagacgc ggaaaatatc
     2341 actacaaaaa gacagctaga tgagctagtc attgaaccat ttgctaatcc tttccgtgtc
     2401 tatcctctta gggaagacta tcaagctttt cgagagttgt ttttgaaaca gcaaacagcc
     2461 tgctatgtcc tgaatactgg atatttcgat gataaaaaaa tcaaacctga aacgacattg
     2521 tcaatcgttg aaagaatcgt gcaacaaacg gcagaattca agtcgtttgg tccatttgaa
     2581 gacatccact atttacctat accagatttt cctgtttctt ttgaggatac gtcctataaa
     2641 aatctggtga aacaacgact tgatattcgt ttacgattca ttgaagctca gataggaaaa
     2701 gatctcttgc cagatgaagc aaaagaaagt ttggagaatt tgattcagaa attgaactaa
     2761 aataacagct ttttcaagaa gtaatccatg cgcaatatcg ctcgggatcc ttacttgaaa
     2821 aagctgtttt tctatgatgg atttttcgta atataggtgg agatgtcctt ttgataatct
     2881 tccgtaggcc aatgaccagt atttaagaag ttggctaata gatagccgat ataaggacct
     2941 gtggtcaagc cagaagagcc tagaccgcta gcagccagta agcttggatc atctggcagt
     3001 acgccgaaaa atggggaaaa atctgacgta taagcacggg tacctacacg gaactcgtat
     3061 ggccagtttt ttatctgatc tggatcagtc aagaactgtt ctgtactctt agtcaatccc
     3121 ttataagctt cgctagtagg agccaaatcc cagccttgat cgttttcatg ggtagcacct
     3181 aaaaggatct ttccatccat gaaaggaatg aaatcagctt ctccgtcaag catagcaaca
     3241 ggccagtctt gactcatctt ataagatgtc tgaaatgcta agagctgccc tttttgcgga
     3301 cgtatatctg cagcatagcc taattcttgt aataaaggct taagtgccgg gccaggtgtc
     3361 aaaattacac gatcagctgt gtattctcca aacggtccct tgatgagcca agaatcgctc
     3421 ttcttatgca aagtgacttt tgcctggatc tgctccattt gtttttgtgc taaacgctga
     3481 ccaagatgtt ctaaatatgc ttttccatcc agacgaccac caccactgat acgcagtgaa
     3541 actgctggtt tcaataacgg aagtgcttga gatgtctgtt caggtgaaag ccattcgatg
     3601 tttccaattt ctggcgcttc ttcttttcgt ttctcggcaa gaacagccaa ttcagctaaa
     3661 tctttttcag gtcgaagcaa aagtgtccct gattgtgcgt aaatcgtttg atcaagttgg
     3721 aaatctttta taagtttagg gaaaaaagca gcgccgtctt tagccagacg ataccattgt
     3781 ttgtttctgc gtttcgacaa ccaaggggag ataatcccag cgctagcttt agttgcctgt
     3841 cctgtccctt catcaaataa agcaacattg aattttgatg tatctaagta actggctagg
     3901 gtcataccaa tgatcccgcc accgataatg gcaatttttt ccataaaaaa gcctcctttt
     3961 acaaacaacc attttcacac aaaaaaacgg cagatgcaac acctgccgta tatggaatct
     4021 acgctttttt agcaaagaac agataatagg cgaatcgagc aaagcttacg agaagcaaca
     4081 gcaacaagac gcctaatgct gcttttgatc ctaatgtagt agacacttca tagttatggg
     4141 aagtatgatg ctggacgtaa ttgataatga ccgctactaa tgaagtagcc acagcgccag
     4201 aaaactgctg caaggtattg aacattgcgt tcccgtctcc ttgtagttct tcattcagtg
     4261 cattcattcc ggtggtcatc atattgctgt aagataatcc gataccgatc atataaaaca
     4321 catgacaagc aatcaataca cccactgaag cgtgtcctat gagtaggaat aatgctagcc
     4381 aaccaatcaa agaaagaatc agcccagcaa aaattggctt cttcggacca tattgatcca
     4441 gcattttccc ggaaaaagga gaaagcaaag caccaaccgc tgcgccggga agcatcgcta
     4501 atccagcgac aaaaggacta cttccttgaa cgatctgaag gaaattcggc aagacaaaag
     4561 atacaccaag taataaaaac tgataaacta agaagccaat caaaaacaag cggaatgttt
     4621 tatttgaaag aatagacagt cggatcagtg gatgctccac ttttttactt cgatagtaaa
     4681 aaaggaacca gccaataccg ccgatgatta aagcaatcca gctggaaact gttcccaacg
     4741 tactcaaaaa tgttagactt ccactaaaca aaagacaaat tcctaaaacg cttatccaat
     4801 ccaaataacc acttttttgt actgccatct cgggaatagc aaaccagccg ataatcaaag
     4861 aaagaatcaa aacgggcaca agaaacagga agatatgatt ccatgtaaaa tgtgaagtca
     4921 taatgcctcc ataagtgggg ccaattgctg gagcaatggc cgttgtcaaa gtaccgacac
     4981 ccatcatcgc gcctcgtcga ttgagggggg caaatgtcaa gataatatgg aacatcaacg
     5041 gaagagcgat acctgtcgct gcaccttgga gcaatcgacc aaacaatagg accatgaagg
     5101 taggagagaa gaaatcgatc attatacctg ccaagaaaag cagatttgaa gctaaaaaga
     5161 gttttttgag actaaaattg cgattcaaat aagtagacag cggaacaatg atcgaaatca
     5221 tcaaaagata aatcgttgtt acccattgga cttgtgcagt cgttaaatca aactgtctga
     5281 taagagttgg gaaagttaca ttcatggccg tctcgatcaa tacgcccgca aaagacatca
     5341 tccctgttgc aattactgca ggtactaaaa gcagctgttt ttgttttgtc atttacatca
     5401 cctctaaaaa atttcgtaaa aaaagagagt gatttctatt ataaaaatag aaaccgcctc
     5461 tctctcgaca cgccgaaacg tgttatctgt acgatttatt tacttcactt attctagcac
     5521 aaactgaaaa atagtgtcaa cacgattagc caagtcactc aaaaagaggt atgataataa
     5581 ataactaaat ggaatttcgc ggaggaagaa aaatggaaaa aattgaatgg cgtaaagaca
     5641 agagctatag tatgaaaaaa agcccgcaaa tactagaggt tcctaaaaaa tcattcttta
     5701 gcatcagagg gatcggcgac ccgaatcagg aagactttaa acgaagagta gcctgtctat
     5761 atgcagtcag ctatgcaata cgcatgtcgc ctaaaaaaaa ttggctgatt cctgattatc
     5821 agatgtatac tgtctatcca ctggaaggac aatggggact gcaagaaaag tatttgcatg
     5881 aaccagtgat gaaaaaagaa catttttctt atcaattgat gatccgacag cctgactttg
     5941 ttacagaagc gatagcacaa gaagccattc agcgttctag caaacttccg gaagacttga
     6001 aagaacaagt agtattcaaa aagatggaag aaggtctttg tgcccaaatc ttacacgtcg
     6061 ggccctatgc cgaagaacca gcaacgtttg agaaattaga agcattcatc gcaaaagaag
     6121 gctatgaaag agcttctaaa gaacacaaag agatttacat cagcgatcca agaaaatcag
     6181 cacctgaaaa aatgaagacg attttgcgtg ttaaaataga aaaaggttag caacctgaat
     6241 gaaaacaaaa aataaaagta aaaaatacca aaatatgaag aaatattact tatcttttgc
     6301 tataatcaac attgagaggt gtctaatatg aaaaagaaac agcgacaagc actgatccgc
     6361 cagatcatta cagaacaacc tattgggaca caggaagagc tgcttgcgcg gctgcatgaa
     6421 gcaggcgccg atgtgactca agcaacgatt tctcgagaca ttcgagaaat gaagttagta
     6481 aaaagtcaaa atgaaaacaa aatagtacgc tatacattat ttaaccagcc atctgtttct
     6541 ttaaatgaag aacgtttgcg tacagcaatt agacgcgagg ttttgcgtat ccagtctgtt
     6601 cagttcatgg ttatcgtatt gactgaacga aatggtgccg atgtggtaac gaattggcta
     6661 gatgaagtgg cttaccctga agttgtcgga acgatggccg gtgtagatac attcattatt
     6721 atttgccgct cagaagaaga agcacagcga tttgcagaaa agctggaaaa aatgagggaa
     6781 taaatgagga ggacttttat atgaaagata tgaaagcgct gcaaaatgga tcggatattc
     6841 gaggaatcgc attaccagtt gaaggatatg aagtaaactt gacgacagac atggtaaaaa
     6901 agatcggctg gggactagca aactggataa agaaaaagaa gaaatcaacc gatccaaata
     6961 aaaaatggac gattgggatt ggtcatgaca gtaggataag cggaccagca ctgaaagagg
     7021 cgttgatcca aagtctgaaa gaacaaggga tacatgtcat cgatttcgaa ttagctacta
     7081 cgcctgctat gtttatgtcc actcaatttg aacagttctc gtgcgatgct gcggttatgc
     7141 tgactgctag ccatcttccc tattattata atggaataaa attctttacc tcagaaggag
     7201 gagcagaaaa agaagatatt gcttttatcc tatcacagca gcaaaaaata aaaagcgaac
     7261 aaactggaag cgtagaaaaa gcagaccttt tgacacctta tgcgcaagat ttagtcacaa
     7321 aaattaaaaa ggggattgga acactagaaa gcaaaccact aaatggttgg aaaatcattg
     7381 ttgatgccgg aaatggtgct ggtggattct ttgctgaaaa agtattgaat agcttaggtg
     7441 cggattcaac aggttcccaa tttttggaac cagatggccg atttcctaat catatcccaa
     7501 atcctgataa taaagaagcg atggaaagta tcaagcaggc tgttttagca aatgaagcag
     7561 atcttgggat cattttcgat acagatgtgg atcgttctgc agttgtcagt gcttctggaa
     7621 cagtgatcaa tcgaaataat ttgatcgcag tattaagtac aatcgtatta aaagagcatc
     7681 caggaacaag aatcgtgacg aattccccta cttcttctca tctacaaaga ttcattgaag
     7741 aaaaaggagg catccaagtt cgttatattt ccggctatcg aaatgtaatc aataaagcat
     7801 tagaacttaa ccgtcttggg aaagatacac agctagccat cgaaacaagc ggacacgctg
     7861 cattcaagga aaactatttt ctggatgatg gtgcatatgt catcgcaaag atcctgatgc
     7921 ttttgccaga attaaaaaga aaaggtatca cattagatga acaaatcaaa gagttgaaac
     7981 agcccgtaga agtacaagaa atccgattaa aattgttcag cgatgaacca cgcgaacaag
     8041 gaaacaagat attagaagaa atgaagcgac tgttggaaga agaaaaaggg atgaacttcg
     8101 acccggaaaa tgaagaaggt attcgatatg atctagaaga gccatacgga aacggctggt
     8161 ttttgatccg tttaagctta catgagccgt tactagtatt acagatagaa aatgatgaat
     8221 tcggaaagaa caagttggcg ttagtaaaat tagcaaaatt attcggacat ttttctgatc
     8281 ttgaatcatt aacagcagaa taaaccagtc tttttgaaag gctagttgct tggacggcaa
     8341 aaaaagtggt atggttaaat cgagtgctgg tagggagacc agcaaaagga ggaaaaaatg
     8401 gtaacgaata tggttatttt tttaacagta ttggtttatg gggtcaactt tctggtatat
     8461 atgtatctta aaatcaatca tataaaagat tatgtagaaa agattgctat ttattttgga
     8521 acaaatatga cgctgttgtt cacgagtagt atttttttgt tttttggaaa aatagtagaa
     8581 gacggcattc tggtattcga ataagcagaa tttttgtaga aagcgcctta cttgtcatac
     8641 actataatta tagccaagta gcaaatggtc aatcgaaagg agcagtacaa tgaaaaaagc
     8701 atacgaaaca gcaatgatca accatggcgg gcgcaatgga gaagtcgagg caccaaatgg
     8761 ctcgatgcat atgaaaatca ctcctccagg aatccatgca gaaggaacaa atccagaaca
     8821 attatttgca gcaggttatg cttcttgttt caatggggcg gtacaacata tgatcaaaga
     8881 agcaggaatg acagcagagt ccacagtaaa agcacgtgtc tctctttata atttagatga
     8941 tggcggctat cagatcggtg tagtattaga agtgcatatc gatggcatca gcgaagaaga
     9001 agcccaaaaa attgcagaag atgcgcatga attctgccct tactccaaag caactagagg
     9061 aaatatcgat gtagaagtaa cgattgttta gaagaataaa ggaaatcgaa ccagactaaa
     9121 aacgtgaaag gaccgctttt tgtctggttt tttttgacaa ataaagcaga tgatttggag
     9181 aatagacaat tatcggttat catatttttg taccaatcgg taggtaaaaa agaagcgagg
     9241 gaaaaatgat ggaaaaatat caaaatattg tgatcggttt tggaaagggc ggtaagactt
     9301 tagcgaaggc gttagcttca aaaggtgaat cggttttagt cgttgaaaag tcaacacgta
     9361 tgtatggcgg aacatgtatc aacatcggct gtattccttc taaatcattg attttcaatg
     9421 gagaaagagg aatcgacttt acagaggctg ttgctcggaa agaaaaattg acgggcatgc
     9481 ttcgcgccaa aaattatcat atgatcagcg atgaggcaac tgggacagtg atggacggta
     9541 cagcacgttt tctttctaac catcaaatcg aagtgacgaa taatggtgaa aaagtgattg
     9601 ttgaaggcga acggattttc atcaatactg gttctgaacc aatcatttta ccaatcaaag
     9661 gattgaatac aagtcgctac ttgattgatt ctacgcaagc gatggatcag gaacaattac
     9721 cggaaaaatt agtgatcatc ggggctggat atattggctt ggaatttgca tcaatgttca
     9781 atgaatacgg atctaaagtt gtcgttcttg atgcccattc tgaatttctt ccacgtgaag
     9841 atgaagatat cgcgcaaatg attttagaag acatgacaaa tgcgggaatc gaattccatc
     9901 taggtgtatc tgtagatcaa gtagcagacc aagaatcaac agctgccgtc acctttacag
     9961 aagatggcca agaagtaaca atccaagcgt caaaagtttt ggctgcaact ggaagaaaac
    10021 caagtacagc atcacttgga ttagaaaata cagatgttaa actaacagat cgcggagcaa
    10081 tcgctgtgga tgaatacctt cgtacagatg cagacaacat ttgggcgatt ggagacgtca
    10141 aaggcggatt gcagttcact tatatctctc tagatgatta tcgaatcatt atggatcagt
    10201 tgaagggcga gaataaacga acaacgaata accggaaagc tgtgccttac agtgtattta
    10261 tcactccgac tttatccaat gtaggtttaa cagaaaaaca agccaaagca caaggcgtca
    10321 attacaaatt attcaagtat atgacaagcg gtgtccctaa agcacaagta ttagaagatc
    10381 ctaaaggtgt attcaaagtt ttagtcgacc ctgaaactga cttgattcta ggggcaagta
    10441 tctatgcgga agattctcat gaagtcatca acttgatttc gctagctatg aatggaaaac
    10501 ttccttatac gttattgaga gatcaaatct attctcatcc gacaatgagt gaagctctaa
    10561 atgatgtatt aaaataaata acgaatggag aatccaccat gttcaataat aaaaatacag
    10621 cagtagttct cactgacccg caagtcgagt tcttgaaacc agcaggcggt ggctttggtt
    10681 tgacaaaaga tatcttagaa aaatacaata cgatcgaaaa tatgattgaa atggtaaaac
    10741 aagcaaaaga aaaaggatac aaactcttta tttctcctca ttatttttac ccacatgacc
    10801 ataaatggca atttggcggt gcaggagaac atatgatgct tgagcagcag atgtttgcta
    10861 gaaaaagtca atatgaatca gtggaagaag gttcaggagc cgattttatc gaagaattga
    10921 aaccttattt ggatgaaaat acgatcatca gctctcctca taaaatcttt ggaccagaaa
    10981 gcaatgattt ggcgctccaa ttgagaaaaa atggaatcga cacagtaatt cttggtggaa
    11041 tgaacggaaa cttgtgtagc gattcccata tgcgtgaatt ggtagagagt ggattcaatg
    11101 ttgtggtagc gacagatgca attggtgctc ctggccaaga agcaatggat gcagcgttga
    11161 cgaactttgg ctttattgca gatcaagtga tgacaacaga ggaagtatta gagcaactat
    11221 cataagatag gaattgcttt tgtcctcgca gttcttgaat caatgaacat ctagctatat
    11281 gagaacagtt ttatagtgag ggctgtgtca gataatttgg cacagtcttt tttaaacgat
    11341 taagaagaaa aaatgatgaa tttactgtat aatataggac tatgagaaat gaaaaaggag
    11401 aatatcattg aaaacaaagg aaaagatctt ggaagcgacc aaagatctta tttataaaaa
    11461 aggctatcat gaaacgtcaa tccgagatat tctagcagct tctgatacag gaaaaggtca
    11521 attttattat tacttttctt caaaaaaaga agtttgtcta gcggtgatcc atgcgcatat
    11581 ggacgtatgg cagacagaat tgttcgaaga catcttaaat gctggaaaac agccagaaga
    11641 agcattagaa gaaatgttgg actggatttt cgctgatcat gagacacaga tcacttatca
    11701 tggctgtcct gtaggtaatt tgattattga gttgagtaca gtggatgaag aataccgtct
    11761 gttgctacta gaattagtca atcaatgggt caagtatacg aaacaaaata tcctggcact
    11821 ttctgaagaa aaactgtccg atgaagaagc agaccgtcaa gccaaagcgt tgattgctga
    11881 aatccaggga agtatcttat tgttgaaagt cacacaggat ctatcactac ttaggcagaa
    11941 ttttcaattg atcaaagaaa gattgttaac ttcgcaaaag gttctttaat aattagaggt
    12001 tgtgacagaa gtggtcagct tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa
    12061 tttttgtgaa atttcggctt atttttcgaa gaagctact
//
LOCUS       DACGLH010000089        11490 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.094, whole genome shotgun sequence.
ACCESSION   DACGLH010000089 DACGLH010000000
VERSION     DACGLH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.094"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            64..2103
                     /locus_tag="IXT21_002459"
     CDS             64..2103
                     /locus_tag="IXT21_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ4869829.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2212..3363)
                     /locus_tag="IXT21_002460"
     CDS             complement(2212..3363)
                     /locus_tag="IXT21_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ4869830.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(3517..4047)
                     /locus_tag="IXT21_002461"
     CDS             complement(3517..4047)
                     /locus_tag="IXT21_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869831.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4146..4496)
                     /locus_tag="IXT21_002462"
     CDS             complement(4146..4496)
                     /locus_tag="IXT21_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ4869832.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(4653..6182)
                     /gene="ahpF"
                     /locus_tag="IXT21_002463"
     CDS             complement(4653..6182)
                     /gene="ahpF"
                     /locus_tag="IXT21_002463"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ4869833.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6192..6755)
                     /gene="ahpC"
                     /locus_tag="IXT21_002464"
     CDS             complement(6192..6755)
                     /gene="ahpC"
                     /locus_tag="IXT21_002464"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ4869834.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(6899..7561)
                     /locus_tag="IXT21_002465"
     CDS             complement(6899..7561)
                     /locus_tag="IXT21_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4869835.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            7745..9391
                     /locus_tag="IXT21_002466"
     CDS             7745..9391
                     /locus_tag="IXT21_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ4869836.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            9382..10464
                     /gene="uxuA"
                     /locus_tag="IXT21_002467"
     CDS             9382..10464
                     /gene="uxuA"
                     /locus_tag="IXT21_002467"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ4869837.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(10572..>11490)
                     /locus_tag="IXT21_002468"
     CDS             complement(10572..>11490)
                     /locus_tag="IXT21_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4869838.1"
                     /translation="GMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDLSVD
                     ILRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESL
                     CTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAI
                     EQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISK
                     DLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKK
                     VIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         3841 a         2140 c         2127 g         3382 t
ORIGIN      
        1 tgttagaata aacaggtata aatagtgaaa taaaggaatg acaagcaaga gaaggaggaa
       61 aaaatgaaaa gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat tgccggcgca
      121 gtgatcttaa tagcagctgc gggtggcggt tatttttact accagcacta tcaagaaacc
      181 caagcagtag aagctggaga aaagacagtt gagcaatttg tccaagcttt gaacaaggga
      241 gattataaca aagctgcaga aatgacatca aaaaaggcgg caaataaaag tgcattatct
      301 gaaaaagaga tcttagataa ataccagaat atatacggtg ctgctgatgt caaaggactt
      361 cagatatcga atctaaaagt agataaaaaa gacgattcta cttacagttt ttcatataaa
      421 gcaaagatga atacctcatt aggtgaattg aaagatcttt cttataaagg aacattagac
      481 agaaatgatg gtcaaaccac gatcaactgg caacccaact tggtttttcc agaaatggaa
      541 ggaaatgaca aagtaagcct gaccacacaa gaagcagcaa gagggaatat tatagatcga
      601 aatggtgaac cattagcaac aacaggcaaa ctaaaacaat taggagtcgt tccaagtaaa
      661 cttggagatg gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc cttcgactta
      721 acagaagatg ctatcaatca ggcgatttca caaagctggg tacaacccga ttactttgtc
      781 ccattgaaaa tcattgatgg agcaacgcca gaacttccag ctggagctac cattcaagaa
      841 gtagacggca gatattatcc tttgggtgaa gcagctgctc agctgattgg ttacgtggga
      901 gatatcacag cagaagatat tgataagaat ccagaattaa gcagtaatgg aaaaatcggt
      961 cgctcaggtt tggagatggc ttttgataag gatcttcgtg ggacaacagg tgggaaatta
     1021 agcattacag atgcagacgg tgtagagaaa aaagttctga tcgaacatga agttcaaaat
     1081 ggcaaagata tcaaattgac aatcgatgcg aaggcacaaa aaacagcttt tgacagtcta
     1141 ggaggaaaag ctgcatcaac tgttgcgaca acgccaaaaa ccggtgatct tcttgcgctt
     1201 gctagctctc caagctatga tccaaacaaa atgacaaacg ggatctcaca agaagattac
     1261 aaagcttatg aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac aggttatgct
     1321 cctggatcta cgtttaaaat gattacagca gcaatcggtc tcgacaacgg cactatcgat
     1381 ccaaatgaag tgttgacgat caacgggctt aaatggcaaa aagacagttc ttggggatct
     1441 tttcaaacaa cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac tgctttgatc
     1501 tattccgata atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa aaaatttcgt
     1561 acaggcttag ataaattcat ttttggtgaa gaccttgatt tgccaattag tatgaatcca
     1621 gcacaaattt ctaatgaaga tagctttaac tcagatatct tgctagctga tactggatat
     1681 ggacagggcg aacttctaat tactcctatc cagcaagcag caatgtattc tgtttttacc
     1741 aacaatggca cacttgtcta tcctaaattg attgcagata aagagacaaa agataagaag
     1801 aatgtcatca gcgaaacagc agtacaaacg atcgtgccag atctgagaga agttgtgcaa
     1861 gatgtaaatg gtacagcaca ttctctttct gctttaggga ttccgttggc agcgaaaact
     1921 ggtacagcgg aaatcaaaga aaaacaggat gtaaaggggc aagagaacag tttcttgttt
     1981 gctttcaatc ttgataacca aggttatatg atggtcagca tgttggaaaa taaagaagat
     2041 gatgattcag caaccaaacg agcatcggaa ctattgcaat acctcaacca aaattatcaa
     2101 taaaaaattg ctttttaagt acagtaaaag gactgtggcg ttatcgtcac agtccttttt
     2161 tcaaataaac ggttttcacg atgtctcttt cacaacttct ttttgattta ctcacacttc
     2221 ataaactttt tttgtaaaat atttgagaac aagtggagaa ataagtgtac ttaataaaat
     2281 cacgataact aaaggtgaat agtactgtgg ttcgatcaat tgactttgct gtccaatttg
     2341 taaaatgatc aaagccattt ctccacgcga aatcatacca gcaccgacca tcaatgaact
     2401 atgttgagaa aaaccggcaa tctgagcacc aaaataacct ccaagcaatt tggtcaaaat
     2461 cgctacaagc gtcaatatag aaataaagag tagttgttca ctaaagtcag aaaaatcaac
     2521 ttctaatcct acactgacaa aaaatactgg tataaatact gcatatccta aagcctctac
     2581 attgtggtat acttcttctt taacctttgt ctgactgaca gctatcccag caaaaaaagc
     2641 accaatgaca gagctaagcc caacaagatc cgctacgtaa gacattccta aacaaataac
     2701 tagcgatacg atgataatag aagaatttgc gtacactttt tctgcaaatg aaagtaggaa
     2761 aggcgcaatc catttgacta ataagaaaat aaaaacaaaa taaattagct gttcaatcaa
     2821 aacaagcggt aaagaagcat tgctagaagc ttcccccgtt agaaaagata ggctgaaact
     2881 tagaactaaa acgactaaaa tgtcatctac cacagaagca cccaaaattg ttgatccttc
     2941 ttttgtattc actacgttta gttcttttaa tacttctact gaaatactga cagaagttgc
     3001 cgcaagaata atcccaaaga aaatcgcttc atttcccgaa acttggaatg ctttgcctgt
     3061 caaccagcca aaaacaaccg ggaagatgat cccaagcaaa gcaacgaaca taccaggtcg
     3121 gaaatatttt ttcaaaagag ataaatcact ttccagacca gctaaaaaca ttaataagat
     3181 cacaccaatc tccgaaaagt catggactag aatatcaggt ttgacccaat tcaatccagc
     3241 ttggcctagc aaaacaccaa caagtaattg tccaatcact gctgggatac ctaatctccg
     3301 cgagatatgt gagccaatcg ttgttgaaat caatatcagg cataaaatac caataaattc
     3361 cattctcatc attcctttgt ataaaatata gcccgaactt agcataacat acattattga
     3421 aaaaatagga aaatatactc acaaaaaaac caacaatcca aaagattttt ttggattgtt
     3481 ggtttttata cggtctattt taataactaa aaatgattaa cgtatagtca tttttcttat
     3541 tctgatgaaa ttgataatct cctggatgcc catgaaaatc aaaataaagc cgaatatttg
     3601 gaatagaagc atcaaacttt tgaatggatt gaacaataag acgaatccgc caatcaacaa
     3661 cagaatacta ctataaacac tccaactttt agcttttgta ttcatgctga agaacaactg
     3721 gaacaagctg ttgatgataa cagctaatcc aagaagaatc ggcaggatag aaacgattgc
     3781 aggtgcaaaa gcatagacga tcagagctaa gatcaggaaa aaaagaccac tgaacagacc
     3841 gatactccaa gaacctgttt tccttttaaa acggtaagcc tcaaaaagat tcaacaaccc
     3901 aagcaaaaca agataggcgg tgatcagata accgataaaa tgaaaaacag cagaagggtt
     3961 tagcacaatc gcaatcccag aaataatata aacagcagct cttaataagg cgtatttttg
     4021 aatattttgt ttaatcgtct caaacatata agaaaacctc ctttgactta ttttaacgtg
     4081 aactagtcat tttaccaaat taaatgttac aaaatgagcg aaggagaaga atatctctgg
     4141 aaaaatcaac ttctgaggat cttttccatt tttttgcctt ttgctagctc atcgatcatt
     4201 ttatctaaat aacgaatttc tttcatcaag ttatcctcga tgtcttctac tcgtacacca
     4261 caaacaacac ctgtaatcaa ggaacgtgaa ggattgggga caggggcttc cgcaaaaaat
     4321 tgttcgaaag tcacttgttt ttgtagttga tattctagtt ctgtttggga ataacccgtc
     4381 agccaacaaa tgatttcatc tacttcctct tttatacgat cttttctctc cgcttttgct
     4441 acatacatag gataaacgct tgcaaaactt gtcgtaaaaa tacgattttg cgccataatc
     4501 agtcaccttt ctttttttca aattatttac tacttatagt atataagaaa aagagaagaa
     4561 aaagcgatta ttcaaaaaaa caaaaaggat tgtgacaaaa ttcttgtcac catccttttt
     4621 tccgctctct gactttttac aatcttcata gctcaagtgc gcatcagata atcgaatgct
     4681 cctaaagctg cagtagctcc tgaacccatt gaaacaatga tttgtttgta tggactatct
     4741 gtacaatcac cagctgcgaa aacgccgggg atattcgttg cgccattttt ctcggtcact
     4801 atttcatttc ttggattcat ttcgatcgtt ccatctagcc aatccgtatt agggagtaag
     4861 ccgactagaa tgaatactcc tgaaacagct agtgtatgca tctccatcgt acggcgatcg
     4921 atataggaga gagattctac ctgattcgta ccgctgattt ctttcgtcgc tgcatgtttg
     4981 ataacagtga cattcgcaag agagactagt ttatcctgta agatttgatc tgcttttaat
     5041 tctggtaaga attctagaac atatacatgt tttgctaacc cagccaaatc gattgctgct
     5101 tcaacaccag aattaccacc accgataaca gcaatctctt ttcctttaaa taaagggccg
     5161 tcacagtgtg ggcaataagc aattccttta tttttaaact ctttttcgcc tggtacattg
     5221 atagaacgcc agtgcgcacc tgtcgcgatg atggcggttt ttgctttaag tgatataccg
     5281 ttagctaagc caatattaat aaaatgttgc tctttattta aggaaacggc ctgttgattc
     5341 atcataatgt cgatagggta actacgcaca tgactttcta cttgctgcat caattttgga
     5401 ccttctgtgt aagaggtacc aatcatattt tcaatcccta gtgtttcaac gacttgtccg
     5461 ccgaaagaat cagcgacaag tcctgtacgg attcctttac gtgctgcata gatagctgca
     5521 ctactagctg ctggtccacc gccaatcact aatacatcat aaggttcttt ttcaaacaac
     5581 tcttcttcaa tcaaagggcc agtgattttt tcaacaattt gttcgatcgt catgcgtccg
     5641 ctactaaatt cttctccatt taaaaacaca gttggcactg ccatgatttt tttatcatcg
     5701 atttcttttt ggaacatccc accttcaatc atcgtatgag taatatttgg atttaataca
     5761 gctaatatat tcaatgcctg tacaacgtcg gggcaattat gacaagtcaa gctaacataa
     5821 ctgtcaaaat gcaatgtttg tgttattttt ttgattttaa gactcaaaga ttcactgatt
     5881 ttcggtgccc gtccactaat ttgtaacata gccagtaaaa aagaggaaaa ttcatgaccg
     5941 agggggatac cagcaaaaac gattccactt tctttgccaa ctggattgat agaaaaacta
     6001 ggtgtgcgca tcgaatctct tttttcccaa gagattttag gagacatttc tgctatttcc
     6061 ttcgtaaatt cagctagttt ttgactattt tcactattat ccaaactaac agaaaaaaca
     6121 ataggagatt caatcagtgc taaatattgt tcgagatttt gtttagtttc tttgtctaac
     6181 atatccacca cctaaatctt tccaacaaga tcaaaacttg gtttcaatgt ttcgccactt
     6241 tctttccatt ttgccggaca aacttcacct gggtgggtac gtacatattg agcagcgcga
     6301 atcttatcaa tcagtgcact agcgtcacga ccgattccat ctgcattgat ttccatagct
     6361 tgaacgattc cgtctggatc aataataaat gttcctcgct gagctaatcc ttgctcttca
     6421 tccaaaacat caaaaatacg agaaatctga tgagacggat caccgatcat cacgtattcg
     6481 attttaccga tagcatctga tgtatcatgc catgctttgt gagtaaagtg ggtatcggta
     6541 gagcaagaaa agacttcaac acctaaagat ttcaatgtac tgtattgttc ttgtaagtct
     6601 tctaattcag taggacaaac aaaagtaaaa tcagctggat agaagcaaag aatactccag
     6661 ttccccataa gatctttttc actgacttct aaaaattctc cttgatgata agcatgtgca
     6721 ttaaattctt cgattttttt cccaatcaat gacatagtaa aacctccaat aataattatt
     6781 tatttataat tattgtaatt tagttggagt tgcaaagtca atggatattt tgttatttaa
     6841 taaagattac tcaaaaacga aggttaatta taaaaataga agaggtcgca agaaaatgct
     6901 actgctggaa atactcggga taacgttgag taacagtttc ctgttctttc agcatcatgt
     6961 gtaagtgcaa tcccgtttga aatcgagctt cttctggttt tttatcaact aaagccttga
     7021 gtaaatcata atgttgttcc atgatcgttt cccatggaag cccctgaatg gtcactcgta
     7081 accagcggaa acgttctaaa tgggtattat ggtcatctaa ccagttccaa acttcttttc
     7141 ttcctgcaat ttcgaaacaa gcttcatgaa ataaattatc tgcacggaaa aaagcgcgtt
     7201 cgtcatttat tttgactgct ttttcttgtt cttctaaaat cgtttctagc actattcgtg
     7261 tttgtgtgtc caatttggaa caacattcaa tcatgatttg ctcttccaac tgttctcgga
     7321 caaagcgagc attttctgca gaaacaagat taattttgga aacatacgta ccactttgag
     7381 gtatcgtata cacgagttct tgctgacgta gttgaatcaa tgcttcgcga atgggagtcc
     7441 ggccgatagc aagatgttcc tccagtgcct tttcagatat tttccttcca ggttctaagt
     7501 cagcatagat aatttgttta cgaatcgctt gataagcttg tgattgcaag ctgcttttca
     7561 tctttattcc tccaaaatca tttctatgaa cgtagtataa cagaagaggt ctgaaaaaca
     7621 atctggtatg tcacattaac tagtaaattt gtataaatag taaaattttg gttgacatag
     7681 cgatttttat tgcataaaat tcacttgaat acaaaaacta atatgttagt ttaggagggg
     7741 aaaaatgatt gatttaaaca actggaggaa ctttatccct gaattatctc aaaaagaagt
     7801 agaaatacct cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa atcctgtctg
     7861 gatccatttc ggtggcggaa atctttatcg aggatttcat gcagaaatcg ctcaacgttt
     7921 agcggatcta ggcgaattgt ccactggtgt cattgtttgt gaaacattcg atgaagaaat
     7981 aattgaaagg gcttaccaag attttgatga tttgattctt gaagtggtca tgccaagcag
     8041 tggagagttc gtcaaacgta tattagctgc gacagctgac agtctgtatt gtcatccaga
     8101 ttgttctgaa cactttgata agatgattcg acactttgaa aatcctagtt tacaattagt
     8161 tacttttgct attactgaaa aaggttatcg attgaaagat acaaataata aatatatagg
     8221 accgattgca gtcgatataa aaaatggtcc aaagaagcca tcacatacaa tgagtattct
     8281 tgctgtgcta ctttataaac gttttttatc tggagcttgg ccgattgcac ttgtctctac
     8341 agataatttt tcacaaaacg gtagtcaatt acgtacttct ttgctaacga tcgcaaaaga
     8401 atggcaaaag gaaggttatg tacaaaatac atttatccaa tacttatcga atgaagaaat
     8461 tgttgcgttt ccatggacga tgattgatcg tatcaccccg aatccttcct tgtcagttag
     8521 caaaatgttg gcaaaagagg gcttttcaaa tatgtcgatt ttccaaaccg caaaaggaaa
     8581 taatattgct ccatttgtca atacagagga catccattat ttaatcatgg aagatcattt
     8641 tcccaatgga cgtccgaaat tcgaaaaagc tggggtattc ttgactgatc gtgcaacggt
     8701 agataaagca gatacgatga aggtgacgac ttgccttaat cctttgcata cgtctttagc
     8761 tattatgggt tgtttactgg ggtataggag catagctgag gagatgaaag atccagattt
     8821 atcgctgcta gtttctgaaa tcgggtacaa tgaaggcctc ccagttgtgg agtctccagg
     8881 tatcattgat ccaaagatct ttctggaaga agtaattact gtgcgctttt ctaatccaaa
     8941 tattcctaat atgcctcagc gaatcgccac ggatacctct caaaaggtgg cgattcgtta
     9001 tggtgagacg attaaaaaat atatggaaat agaagttaaa gacccgcaga cacttgtatt
     9061 cataccattg actctcgctg cttggcttcg ataccttatt ggcattgatg atcaaggaaa
     9121 accattcatc ccgagtccag acccactatt gacagacctt cagcataagc tttccgatat
     9181 tcaactgggt acgcaagata catcacatat tcatgagaaa atggctccta ttttgtcaaa
     9241 taaagaaatt tttggtgtag atctttacca agcagagctt ggcagaaaaa tagaagaaat
     9301 gttcatcaag atggtagcag gaccaaatgc cgtacgaaaa acattacagg attacttaca
     9361 agaacatgga ggaaatttat catggaaatg acttttagat ggtacggatc acaggaagaa
     9421 aagattacct tagatgaaat cagacagatt cctgcggtca aagggattgt aggcacattg
     9481 atggatatac cagtaggaga ggtatggcct agggagagga tcaaagaact aaaagaagaa
     9541 attgagtctc gtgggttaac actaaaagta attgaaagcg tcaatgtgca tgatgccatt
     9601 aaaattggat taccttcccg tgagcagtat atcgaaaact ataagcagac aatccgaaac
     9661 ttggcagaat atggaataga agtcatctgc tataatttta tgcctgtatt tgactgggtg
     9721 aaatctgacc ttgactaccg cttagaagac ggctcatcca cgttagcttt tatatctgat
     9781 gatattccag atgatccaag agaaatcgtc gcgcgcatcg aacattccag caatgaattt
     9841 ggattgccgg gatgggaacc tgagcgctta gctcctatca aatccttgct tgaagcctat
     9901 gcatcagtag acgaagaaaa acttcgagaa aattttgcct attttctcca atcaatcatc
     9961 ccaacatgtg aagaggtagg ggtcaaaatg gctgtacatc cagatgatcc gccatactcg
    10021 atttttggat tagctcgaat cgtaaagaat cgagaagatt tagattggtt gtgtcgcata
    10081 gtagacagcc cttataacgg aatcactctt tgtacaggga gtattgcaga agatccagaa
    10141 aatgatgtat atgggatttt agcagaattt tgccaaagag atcgaatccc atttgcacat
    10201 gttcgaaata ttaaattttt agctaatcga gatttctatg aggcaccaca taaatccgaa
    10261 tacggttcat tagacatgta ccgcatacta aaaacaatgt atgacaacgg atttaacgga
    10321 tacatccgtc ctgatcatgg aagaatgatc tggaatgaga caggaagacc aggatacggc
    10381 ttatatgatc gagcattagg tgctgtttat ctaaatggat tatgggaagc aatcgaaaaa
    10441 ggaaacaaaa gtaaagataa ttagtgaaaa aaactgtaat tttattatgt aaataagaag
    10501 gatgttcaaa ttcttgatac ataaatccgg aggttgttaa taagcattcg gatttatgta
    10561 tcttatttgg tctaggaagt aacttgattt tcttttttcg attgatccat gagtgcttct
    10621 tcttttaatt caaagatata ttctgagatc ctcataatca cttttttcca gtcttctggt
    10681 gtgatgattc cttttaaaat atagattttt ttgatatgag aaggcaattg tttcaaaggg
    10741 cagtcgctga taacgatgtt gcagttctcc ataaaatcat tttgtatttt gatcgattca
    10801 tttgaaaaaa tttgttctat cttaacgatc agatctttac tgatataaaa atcacgcgta
    10861 tagttcacac caatcacaat tcgcggctgt tttttgtagc gatcatacaa atgtactagg
    10921 tcttcaacca tccattgcac attttccttt tctaaaataa ctggtaacaa gtgttgattt
    10981 tgttctctga aatccatata aaattgttca atggcggttt ggacttcttt caatacgcta
    11041 tgttcttgat aaagcatgaa agtaggaagg ggcgtatttg gttgattgaa gttaagaaat
    11101 ccattataaa gtttatttcg tgcgaatgcc aatgagtaaa gaatcttttc tttttcaggt
    11161 ataaacaaat caaatacagt acaaagggac tccaccattt ctcttgaata aagaacatga
    11221 ggaacgtttg ctttttttaa aagtttgtat tgctccaagc ttgtttcttc cgattcgatt
    11281 ttcgtgatag aaagtcgagc taaaatattc actaagatgc gttgttcgcg acaaattggc
    11341 agattaggtc gcaaaatatc tacagagaga tcaataaaaa agtcaatata ttcatactct
    11401 gtgaattctt tttgtacgtc tttcacagaa gttgcaggaa aattctcttt acatattttt
    11461 aatagaaaat ataatttatc tatcataccg
//
LOCUS       DACGLH010000090        11242 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.095, whole genome shotgun sequence.
ACCESSION   DACGLH010000090 DACGLH010000000
VERSION     DACGLH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.095"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(99..1229)
                     /locus_tag="IXT21_002469"
     CDS             complement(99..1229)
                     /locus_tag="IXT21_002469"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ4869839.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1788..2861)
                     /locus_tag="IXT21_002470"
     CDS             complement(1788..2861)
                     /locus_tag="IXT21_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4869840.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRK"
     gene            complement(2858..3685)
                     /locus_tag="IXT21_002471"
     CDS             complement(2858..3685)
                     /locus_tag="IXT21_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4869841.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(3686..4492)
                     /locus_tag="IXT21_002472"
     CDS             complement(3686..4492)
                     /locus_tag="IXT21_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4869842.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(4504..5589)
                     /locus_tag="IXT21_002473"
     CDS             complement(4504..5589)
                     /locus_tag="IXT21_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869843.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(5606..6148)
                     /locus_tag="IXT21_002474"
     CDS             complement(5606..6148)
                     /locus_tag="IXT21_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4869844.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            6406..7395
                     /locus_tag="IXT21_002475"
     CDS             6406..7395
                     /locus_tag="IXT21_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ4869845.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7392..8129
                     /locus_tag="IXT21_002476"
     CDS             7392..8129
                     /locus_tag="IXT21_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ4869846.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            8126..8677
                     /gene="coaC"
                     /locus_tag="IXT21_002477"
     CDS             8126..8677
                     /gene="coaC"
                     /locus_tag="IXT21_002477"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ4869847.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            8693..9325
                     /locus_tag="IXT21_002478"
     CDS             8693..9325
                     /locus_tag="IXT21_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4869848.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            9410..10057
                     /locus_tag="IXT21_002479"
     CDS             9410..10057
                     /locus_tag="IXT21_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4869849.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            10062..11045
                     /locus_tag="IXT21_002480"
     CDS             10062..11045
                     /locus_tag="IXT21_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ4869850.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         3445 a         2291 c         2024 g         3482 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaatt ctaagaatag gtcattatag atgcctaggt atttcgatcc tagatctttg
     1861 tagacttcaa gatgggagat cgtgtcatca gatggataga attgttcgtc cgacgaaatg
     1921 tcttttggca ataacgcttt tgcttcttca tttggtgtag aataaccaat atactctgca
     1981 ttttgtgccg cattttccgg ttctagcata aagttgatga aatcataggc accttcttta
     2041 tttttcacgg tttttggcat aacgatatta tcaaaccaaa gattggatcc ttctggtggg
     2101 atcacataat gtaaatgttc attttcccac atcatatcgg cagcttcgcc agaaaatgtc
     2161 actgccacgg aggcttcttc attgatcata tacattttga tctcatctgc cacgattgct
     2221 ttgacatttg gtgttaaatg attcaattta tttgctgctt ctgtcagctg tgccatattc
     2281 ttactattca aagaatagcc taaactattc aaagaaagcc ccataacttc tcgtgcaccg
     2341 tcaatcagca tcaaactgtc ttttaattcc ggcttccaca aatcatccca atgcttaatt
     2401 tgatttgcat caaagaattt atcgttatat acgatcccta aagttcccca aaaatatgga
     2461 atcgaatact gattatctgg atcaaaatcc aagtctaaaa atcgtggatc aatattattt
     2521 aatcctttga tcttcgaatg atctaatgga agtactagtt tttctttgat cattttttgg
     2581 atcatatatt cacttggtat agcgatgtcg tagtttgtcc caccttgctg gatttttgtg
     2641 aacatcgctt cattagaatc aaatgtttca taattcactt tgtatccata ttctttttca
     2701 aatttcgtga tcaaactagg atcgatataa tctccccagt tatagatggt aagtaccttt
     2761 gctccactca tgccacttgt tttttcaagt tgacgaacac caagcagcag caccgcaata
     2821 atgatgagaa tacctaaaaa cagagattgt aattttttca tcgcagccgt gccaccccct
     2881 cgcgtactct tctgtttttc ttcagccgtt ttggacggtt ttctttacta ataaagtagt
     2941 atccaatgac caatatcatg gagaacaaga aaactaacgc acttagcgca ttgatttcta
     3001 aactgattcc ttgtcttgca cgagaataga tctctactga gagcgtcgta aagccatttc
     3061 ctgtgacgaa aaatgtaacc gcaaaatcat caagagaata ggtaaatgcc ataaaatatc
     3121 cagctatgat tcctggagaa agataaggca aaatgatatt tttgactact tggaaattat
     3181 tagcaccaag gtctctagcc gcatcgacca ttgaatcgtt catttcctgc aattttggca
     3241 agaccatcaa gacgacgatt ggaatgctga aagcaatatg ggaaagcaat acactctgaa
     3301 aacctaatcc gaatcctagt agtgtaaaga aaatcaagaa actagcaccg ataataacat
     3361 caggagagac taataagatg ttattcatgc tcaacaacgc tgtccgtgca cgtcgttttt
     3421 tcgtgtaata gattcccata gccccaaatg tcccgatgat cgtggcgatc aatgcagata
     3481 agaaagccag catgaacgta ttcaatacga taatgatcaa acgtgtatct tcaaaaacag
     3541 cttcataatt ttcccaagta aatcctgtaa agctgttcat cgttcctcct tcattgaagg
     3601 aataaaaaat cagatagaag atcggtgcat ataagaataa aaagacgatc cctaaataaa
     3661 gatacgacca tttaaatttt ttcatttatg agcgacctcc ttttttcttc tcgcctgtca
     3721 ggagcatcac gatgaacata gcaatgatta aaatcacacc gattgtagaa cccatccccc
     3781 agttttgtgt aaccaagaaa tgctgttcga tagccgttcc taaagtgatc acccgatttc
     3841 cgccaatcag acgagtcagc atgaataaag aaagagaagg aataaatact gcttggacac
     3901 cacttttcac accatttaat gataacggaa aaatcactcg tcggaaggtc tcaaagctat
     3961 tcgcgcctaa atctctacta gcactgatca aagaaggatt catctcttcc aatgcattga
     4021 aaattggcat aatcataaat ggaatctcga tatacgttgc cacgaacaga aaactgaagt
     4081 ctgtgaataa tatctgtttc ggcccgatcc cgataaaatc aaagaaatga ttcacgctgc
     4141 cgtgcacact gaaaatccca ataaatgcgt aagctttaag cagcagattc acccatgtag
     4201 gcaaaataat cagcatcagc cataattgct tgtgcttcag ttttgttaaa aaataagcag
     4261 tcgggtaact gatgatcagc gtgaaaaacg tgatcaaaaa tgcataccat acagaattga
     4321 tcgtcataga aagcgatgtc cccgacgtaa aataagatag ataattattc agcgtgaatt
     4381 gcccattcat atcaaaaaaa gattgataaa tgatcattaa tacaggcgcg atcacgaata
     4441 aaccgagcca caaaatataa gggacggaat agatcctgcg cattgttttc attcttgtcc
     4501 cccctattct tcgtagctgt caagacgtgc atcgaattct tcttcagact cgttgaaacg
     4561 catgacatgg atatcttctg gctcaaaggc caatcctact ttgcttcctt ctttagcttt
     4621 tttcgttgaa tggaccatcc attcattttg ctgttcatca taacagatga tttcataatg
     4681 gacaccacga aacagctgag tatcgaccgt tacgactagt tttcctttat ctggtgtcgt
     4741 gatcgtcaaa tcctctgggc gtaaaacaac ttcaactggt tcatttagac gcatcccgcc
     4801 atcgacacat tcaaattgct taccaacaaa ctcaactaga ttatcttcga tcattacacc
     4861 gtccacgata ttgctctccc caacaaaatc agcaacgaaa tgattgattg gctcatcata
     4921 aatatctacg ggtgtaccac tttggacgat tttcccttta ttcatgacaa aaatctcatc
     4981 actcattgcc aacgcttctt cttggtcatg agtaacaaaa ataaacgtga ttcctaatcg
     5041 ttgttgcaga tcccgcagtt catactgcat atctgtacgt agtttcaaat caagcgccga
     5101 taacggttca tctaatagca atactttcgg ttcatttaca attgctcgtg caatcgctac
     5161 acgctgtctt tgaccaccag acatttcact aatctcccgt ttttcgtatc caggaagctg
     5221 gaccatacgc aaagcatctt ttaccttctt ttcgatatct gatttggaca ttttcttgat
     5281 cttcaatcca aaagcaacgt tatcaaaaac attcatatga ggaaaaagtg cataatcttg
     5341 gaagactgta tttacttgtc gcttattagc tggcaaatca ttcaaccgtt tcccttcaaa
     5401 atagatgtcc ccttctgtag cttcggtgaa tccagctata atacgtagga tcgttgtttt
     5461 accacagccg gaaggaccaa gcagtgtata aaattttcct tgttcgatct caaaacttac
     5521 tttttttaat actgtttcat catcatagcg tttcactact tgatcaaatg aaatgattgg
     5581 tttccccacg tttttacccc ctatattaca aatatgattg cgttgcaaca atcaatactt
     5641 ttgttcgttt tttgagatga ttccttaatt gatgttgttt tgtcgcttga taatagatcg
     5701 actgtcctgc ttttgccaca tagaccgtct ctcctaagct taactctaat tccccttcta
     5761 atacaaaaat aaacgtttca gataatgaag gttcaaacgt cttatattct cctgcacctt
     5821 caaaagtcag caagacaggc tccatttctt tttcattcga atcagccacg agccattcca
     5881 attcataccc gttttcttca tcgaaataaa ccgtatgatc ttcttttgaa taaaccactt
     5941 gcagattcgc tgcttcttga tgaaaaaatt cttctggagt cacacctaag acttcaagaa
     6001 tagaaaagaa tgtttccatt gatggtgaac ttaaatctcg ttccaactgg gaaatatacc
     6061 cttttgacaa atctgtgcgt tcgcctagtt cttcttgtgt caaattcttt tggatgcgca
     6121 aatttcttaa cttctcacca atttccataa tttcctccga atcgaagttt tctaatagta
     6181 aacttcctgt ttactaccac tcgtattagt atacgaaatt tattgaaaaa ggcaaccctt
     6241 tgtaaaattt tttttgctga atatttcttt atcttttgga ataaattcta gttgtttgat
     6301 tctttatttt tcacctaatt tttgtctaaa tttacggaag aacaagaata aaagagtagg
     6361 ctttatggta tgataaaaga aaattttaaa aggagtgtct cacgaatgaa cgcttttcga
     6421 tcttttcagg taataaagga acctgttttc catacagcaa tcagtgatct gccatttact
     6481 gagttggctg aaaatgaagt cttggtaaaa attgcctatt ctgatgtcaa ttataaagat
     6541 gcattggcaa tgagcgagtc tggtcaggtg atacgaacat atccgatgac tccagggatt
     6601 gatttatccg gaactgtcgt ccaaagtaac aatccacgtt tttcaaaaga agatcttgta
     6661 cttgctactg gttttgggct tggcgtcacc catccaggcg gttatagcca atatcaaaaa
     6721 gtaccaggag actggttagt tcctctacca aaaaatatga cgctgcgtca agcgatgatc
     6781 cttggtacag cagggtttac tgctatgttg tgtgtgaacg ctttaatccg tcaaggtatg
     6841 acctccgata aaaaagttgt cgttactggt gcttctggcg gtgtaggcag tacagcgata
     6901 gccctgttac ataaactagg attccagtca atcattgcct tttccagaaa agaagagtct
     6961 gtcacatggc taaaaagtct aggtgctttg caagtcgtac gacctgatga atttctgcca
     7021 gagacaacaa aagctttagg aaaacagcaa atcgactatg tcatcgacac agtaggtggc
     7081 gagcagctaa gcagtcttct tccattgatc tcttataacg gtgcagtagc tttatgtggc
     7141 aatgctgggg gaatcaaact aaacgcgact gtcttgcctt ttattctgcg aaacatccag
     7201 ttgattggaa ttgattctgt caatgtacca atagatcaac gtctttcttt atggcaacag
     7261 atggcggatt tgcagattgc agatgaactg gtcgtccaag agattactct ggatcaattg
     7321 cctgaaacag catctaaact acttgctgga acacaccaag gaagaacact agtaaatgta
     7381 ggtgaccata aatgaagatt ctaatcacag ctggaggaac gactgaaaaa atcgatcaag
     7441 tacgtgctat tacaaatcat tcgacaggac gattagggca agcattagct gattatttag
     7501 ctgccaatcc ggataccaca atcgattacg tgactacgaa acaagcactg aaaccgaaaa
     7561 gacactccaa tataacgatt tatacgatcg aatctgcact ggacttattt ttacaattag
     7621 aagaactaac gaaaaaagaa cactacgatg cgattatcca cagtatggca gtcagtgatt
     7681 ttactcctgc ttttagtttt tcagaagaac agctggcaaa aaacctaccc acttcttcaa
     7741 cgcaagaaga actggacaat tggtttgcgg aaaatgaaca gacagatacc actgtttcaa
     7801 agatctcttc taacaccgag catcttgtgc tcgttttgaa gaaaacacca aaaatcattt
     7861 cttatctacg tgaatggcag ccaaaagcaa aaatcattgg tttcaaacta ttagtagatg
     7921 taccaaagga atctcttctg gcagtcgcaa aaaatagtct gataaataac aaaactgatt
     7981 ttattttcgc aaatgatttg acggaaatcc atggcgagac acaccacggc tatctcttat
     8041 ctaaagatgg aacagtggaa gaagctcaaa gtaaatctga gattgctgca ttgattactg
     8101 agaaaattag actggaggaa tcaaaatgaa aaagatctta ctaggcgtta ctggaagtat
     8161 ttctgcttac aaaagcgctg atcttacaag tcaattcgtc aaattaggtg cccaggtaga
     8221 tgtgattatg tctaaaagca gcacacattt tatcacgcca ttaaccttgc aatctctgtc
     8281 gaagcgcgca gtccatacag acgtcatgca agaatcaaat ccgcaggtaa tcaatcatat
     8341 cgaactagca aaacaagctg acttgtttct catcgcgcct gctacagcaa acattatcgg
     8401 caagctagca aacggcattg cagacgatct tctttcaaca gtcgcaatgg cgcttccagt
     8461 gggaacacca aaattgattg cccctgccat gaacacgaat atgtatcaaa acccgatcac
     8521 tcagcgtaat ctggaaacat tgagatctgt cggctatcag gaaattgaac caagagaatc
     8581 tcttttagct tgtggagatt ttggaaaagg tgccttagct gatattcaga cgatcatcga
     8641 acaggtcatg ctgactctta atgaaagaaa tgcttagaaa aaaggagtac ttatgaaaaa
     8701 tacaaaaaac tttacattaa ctgcgatgtt tttagctatt ttgattttat tagctgttac
     8761 gcctttaggt tttatcccta tcggtccaat caatgcaaca accatgcata ttcctgtaat
     8821 cattgcttcg atcgttttag gaccaagact aggtgctttt cttggcggta catttggact
     8881 gatcagcatg atccgtagca cagttatcca aacacctttg tcttttgtct tttctccgtt
     8941 tatcccagtc atcggaacag aacatggaag ctggaaagcc ttgctgatcg cgtttattcc
     9001 tcgcatcttg attggggtgg ttccttattt cgtttataaa ggtctgcgca aactaatgaa
     9061 acaaaaagca gattccgttt ctttattctt agctggactt tccggttcat tgaccaatac
     9121 gattttagtg atgaatatga tctacttttt attccagcaa gattatgcca aagtcatcgg
     9181 aacaaattta aatgctgttt attcagctat tctagcggtt atttttacaa gtggtatacc
     9241 tgaaggaatc gtcgcaggtc ttgcgactgc agcagtatgc aatgtcttgt taagatactt
     9301 caagcaccct tctcttacta actgatagtc atcgtagccg atctctgatt tttttctctt
     9361 ttggtgttac tattgaatgg aaagatttga cgtcgaaagg atgatccaaa tgaaaatttt
     9421 tgttgtaggt gcaaacggac aaattggccg acacctgata aaagatttag ctccaagttc
     9481 ccatgaaatt tttgcagggg tgagagatgt tgcttcccaa acattagtaa aaaaagagaa
     9541 tgtttcgtat gtctcttttg atttaacttg gtccgtggaa aaaatgaccg aagcattcaa
     9601 aggcatcgat gtattgattt ttgctgccgg ctctcaagga aagaatcttc tgcaagtaga
     9661 cttggatggc gcaatcaaaa cagtgatcgc agctgaaaat gcgcatgtct ctcgctatct
     9721 gatggtcagt gctgtttatg cagatgaacc agcaaaatgg cctgagtcca tgacagatta
     9781 ttatattact aaacattatg cagatgaatg gctgaaacga actaacttag attttgtcat
     9841 tcttcaacca gtcacattga ctaacgatga agaagttact tccattcaat taactaagcc
     9901 taatgagaag gcatcaaaaa cgatcactcg ctctacagtt gctgctgtac ttgccgctct
     9961 agtggaagaa acggatattt ctcgtacgac tctagtactt tctgaaggta gcaaagagtt
    10021 gaatacagca ttccaagaat gggcaaagga ggaatagatc catgcgtgcc atttcaatca
    10081 agcatcctgg ttctgtagat gtattagaac aaattgaagt gcctgttcct aaaccaaaac
    10141 aaggcgaatt actgatcaaa gtccatacag cagctgtcaa tcgaacagat attatgaaaa
    10201 gagaatcagt ctctttgaaa cctccctacc ctattttggg agtcgaagta gctggagagg
    10261 tagttgagaa taaaagtgat atctccactt ttactcctgg gacaaaagta tacggattag
    10321 tcaatctagg tggttatgcc gaatatgctg tgatgcctgc cgatcgtgca atccttctcc
    10381 ctgatacgct agactatgtt tctgctgctg gaatctcaga agtttttttg actgcttatc
    10441 aaacacttta ctggcttgga aaattacaag aaggcgaaac tgtcttgatc catgcaggag
    10501 caagtggtgt gggaacagca gcgatccaat tagctagaca gctttctcac gcgaaaatca
    10561 tcgttactgc aggatcagaa gaaaaactcg ccttttgtaa agaacttggt gcagatgaat
    10621 tgatcaacta taaaaaacaa gactttcaag aagaggtacg aaaaatcact gaaggtcggg
    10681 gagtcgatgt tattttggat ttcatcggcg cttcttactg ggaaaagaat ttagcttcta
    10741 tagccgttga tggacgttgg gtcttgattg gtatgctagg tgggaccatc gtgcctgaga
    10801 tagaccttgg tgtattgatt gggaaacgtg tccaactgct tggtacatta ttgacaccta
    10861 gaagtgatca gtataaagca gagctcgcac aaacttttat gcaaaaagct gggccttttc
    10921 tagaaaatgg caagattcgg ccaatcattg accgtacatt ccaattcagc gcagtcaaac
    10981 aagcgcatga atatatggaa gcaaacaaaa acatcggaaa aatcattttg aagataactg
    11041 aataaaatca tgctgatcaa aaaagaggct gggacagaag cgtttaactc caagaaataa
    11101 gaagaaattc acgaaaattg cttttcaaat ttttgtgaat ttcagcttat ttccgaagga
    11161 atttcttctg cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact
    11221 tttgtcccac gctctttttc cg
//
LOCUS       DACGLH010000091        10999 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.096, whole genome shotgun sequence.
ACCESSION   DACGLH010000091 DACGLH010000000
VERSION     DACGLH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.096"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(201..1073)
                     /locus_tag="IXT21_002481"
     CDS             complement(201..1073)
                     /locus_tag="IXT21_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4869851.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGI
                     IGGLALARKAIRNK"
     gene            complement(1112..2230)
                     /locus_tag="IXT21_002482"
     CDS             complement(1112..2230)
                     /locus_tag="IXT21_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ4869852.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2227..4326)
                     /locus_tag="IXT21_002483"
     CDS             complement(2227..4326)
                     /locus_tag="IXT21_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ4869853.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETMNYFAQLFGQLSMSDDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEALEQLSLSERYKDIACKLKESICKQMTIDGTF
                     GEQFLEGIGDEEKTTYSVKNYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDPLYQNTMTFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNH
                     QAFVTRLEELICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIYIQRRKR"
     gene            complement(4377..5198)
                     /locus_tag="IXT21_002484"
     CDS             complement(4377..5198)
                     /locus_tag="IXT21_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4869854.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5195..6004)
                     /locus_tag="IXT21_002485"
     CDS             complement(5195..6004)
                     /locus_tag="IXT21_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4869855.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6025..6519)
                     /locus_tag="IXT21_002486"
     CDS             complement(6025..6519)
                     /locus_tag="IXT21_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4869856.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6531..7019)
                     /locus_tag="IXT21_002487"
     CDS             complement(6531..7019)
                     /locus_tag="IXT21_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4869857.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7096..9714)
                     /locus_tag="IXT21_002488"
     CDS             complement(7096..9714)
                     /locus_tag="IXT21_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4869858.1"
                     /translation="MKKSAKDRVFDELIRLTGNKSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEN
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKDENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     DYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLEHILSP
                     EMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
     gene            10197..10412
                     /locus_tag="IXT21_002489"
     CDS             10197..10412
                     /locus_tag="IXT21_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ4869859.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            complement(10653..10839)
                     /locus_tag="IXT21_002490"
                     /pseudo
     CDS             complement(10653..10839)
                     /locus_tag="IXT21_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         3306 a         2480 c         1785 g         3428 t
ORIGIN      
        1 aacggttgtg taaaaatatc attttagagc atgaaaaaag gagtggattt ctccactcca
       61 aaaaagtcta acttttgggg gtcactacag tttgctcgat ccttttttat ttgaattcat
      121 tttcgcttta tcaaacatca tttgattact cagttaaata cttttgacat gagtaccaat
      181 gaaacattta ttcattaaaa ttacttattt cttatcgcct ttctagccaa tgccaatccg
      241 ccgattattc cagcactatc acctaattcg ggcgtaacaa tgtagctagc aattggtggt
      301 atttcaacat atccatttat aagctcaaca aatttatcct gaactttttt cttcatgtgt
      361 tcttgtttca ttacaccacc gccaaaaata attctatctg gcgaaagcat cagtgttgta
      421 ttgtatgcac attgggcaag gtaaaaggct tcaatctgcc aaaacgaatc gtcagccaat
      481 aaattctggc cttttacacc caatcgtttt tcaatcgctg gtcctgctgc catcccctct
      541 aaacaatctt ggtggaaggg acagtttcct gaaaaattgt cctttggatg tcgcttgatc
      601 aacatatgcc ccatttccgg atgactgaac ccttccacga aagtcccatt gaacaaggca
      661 ccacctccga tacctgttcc cacagtgaag tatgcaacac ctgctaaccc tttgccagaa
      721 ccagcaacat attcaccata gcaagccgca ttgacatctg ttgtccattc aatcggaata
      781 ttgaaatatt gctttagcgt tcctacaaaa ttgaaatttt gccaagcaag ttttggtgtt
      841 gatgtgatat gaccgaaact cgttgatttg cattgaatat caattggacc aaagcagccg
      901 accccgatgc ctgccaattg ttccttaaaa ggtttaaaaa aatcaatgac ctgcttcatt
      961 gtttcaactg gagttgttgt gggaaaactg actctttcca aaatcgttaa ttcctcagta
     1021 ccgactgcac agacaaattt agtccctcca gcttcaattg ccccatatat catcataggt
     1081 tcctcccttc gaatatgtga aaaacacttc attaacgcgg caagcgttct cgatcagtga
     1141 aatgacgttt agtatttcct gaagcttctg ttgaatacca ataggcgaca ctagcaacat
     1201 cgtcttgccg ctcaaataag cgaacatcat cgttgccgat ttgttgtaaa gtgaccgata
     1261 aatcttcggt aaaacagatg gggtcttgaa gatgccaacg ataaagacca tgtcgtggca
     1321 ttgcgtcgtc accaaaggca tgaacggcat tcaattttcc cgtttcaaaa cgatctcttg
     1381 tggcatctct atttgattgg taaggatacc ctaaaaaggc tgttgaatag tttttcgcat
     1441 gtggacgtcc gaactcatcg tactcatgaa aggcccacgc cccgccaaaa taatcttctg
     1501 aacctgttga ggttacggtc ggatactcac tatccccatc aatgtagaat ttaaactcgc
     1561 cttcgcccca ccaataacgt tctaggcagg taagcgcaag gtaagtaccc acgtaatagc
     1621 cttgaccctt taatcgatca attaacttat aatcctcctg taatttggta atgcgttccc
     1681 gatgccaatt tgcgtgaaaa tataggctat tttcttccaa ttcgtccacc aaagcgtaat
     1741 ttatcgtata aaaaaatgag gacatatcct tcggatgtaa attctcgatc gtaattttag
     1801 cggatgtttg gaaaggcatt tcgaaatagg aattaaagcc ccccgtagga ttaaccacta
     1861 taggcaacga gttcacctcg caacgctctc caaacccatt acaaaagaaa tcccctaaag
     1921 gactttcaac agccgggcta tccgacccat cccaataaat tcttaagatt aaatcacgta
     1981 aaacaaaact gccaaattct gttcgatcac gaatcgtcat ccacatatga cgaattattc
     2041 ccgttccagt aatatccgca atcacagttt cttcaccaga agtcagcgga attgcgccac
     2101 gacctttgcg gcttggtcct agctcgcttg gcaacatcgc agctttacct ttctcgcctg
     2161 ttggattttc gggagtgatt gctcgactct ggattttttt aaattgggta atatttttta
     2221 gtatactcat ctttttctcc tttgaatgta aatcgttttt cccttttcta cgtgaagaat
     2281 ttttttcgta tcacttattt tcaatggctt ttctgacaaa tttgaaatac tcaactcgga
     2341 ttgcgtacat tcaatcgaaa tgaaggcttg accgaacttc aaatttttcc aagaaaacgt
     2401 taaattatca acaggatcaa tttgcaatac tccttttttg aatttcaatc cgaaatattg
     2461 actgatcatt atactgacaa aaaggcccga tgcccagccg catttgccgc agccaaagtc
     2521 tcgcacaact tctccttgtt tagcctctaa acgatacggc caccaccacc atgaaccgtc
     2581 taaatctgcc aaacaaatca gctcctctaa tcgggttacg aaggcttgat ggttgtgcat
     2641 gtcactcatt aaaacagtga taaagccagg aaaagtggcg ccggattcta atccccaagt
     2701 gatgcctttg atttcctcgc tataagttgg attgaaagaa ctagcagaaa aggtcatggt
     2761 gttctgatat aaaggatctt tattatctaa aaattgataa aaaggcatca acgtagtgtc
     2821 tgattcttct ccatcatgca tcaataaatt gattttccca tccacaatca catcacttaa
     2881 aaagattaac ccttgctcaa gaatcggttt ttgatagttc ttcactgaat aggttgtttt
     2941 ctcctcatca ccgatcccct ccaaaaattg ctccccaaaa gtaccatcta tggtcatttg
     3001 tttacaaatt gattccttta gtttacaggc gatgtctttg tatcgctcgc taagactcag
     3061 ttgttctagg gcttcaaaaa tttctgcaag tccactacag gccttccaaa gacaaagatt
     3121 gctgccggta tgatactttc caagtgcaaa ggcgtctgat aaccaaacag atggaaaaag
     3181 cattggttcc cctgaccgac gttgagctaa tatgtcctca ataattcttg aagcattcag
     3241 tagaacctgc ggacaccgct caaagaattt tgtatcccca gtatgttcaa aataaagact
     3301 agccagtaac gcactggata acgaattact cagagaatga ttgatacctc ctgtaaattt
     3361 tgaaccagga aacttaacgc catattgatc aaaccataga accgtttttt gacataactc
     3421 cggttcaaac atggtgaatg gcaatgatgc gtaatacata tccttattcc aaattcgatt
     3481 agtagcagga tagctgcccc aattgctgcc gacgacctgt ccctgtcgat tcataccgaa
     3541 actagaaaag ctttgataaa gtgcacgacg atagagatgg acaactctat cgtctgacat
     3601 tgacagctgc ccaaatagct gagcaaaata gttcattgtt tccttcagcc atttttcgat
     3661 atcagattcc tgaaaagtca ttacttcttc aaaagcattt gggtccacaa aagcaatggc
     3721 gaagcttgcc acttcctgtt gtgcgatcgt gtccttattt cctttttgag aaatcaacac
     3781 attttgctga tccgaatact tctcttggta caaaggcaca tctttcactg aaacttgaag
     3841 gctttcgtgt gtttcattca caatgtaaac ctgttgaaca agcatactta aacgcttatt
     3901 agccagtatt ggagcaaagg atactacaat gaccttaaac gtcgaataat gatgaatata
     3961 tctaggcaaa cagtcttgaa tcacatcgac ttcaatacga tgatccgatt ctgacaaatg
     4021 gtagacaatc ccgtttactt caacctttaa ggataacgaa ttggtttgag aaagctgttt
     4081 attgacccaa acacctggtt tttcctcagt tagtaaattt tctcgataaa aggtcaacgt
     4141 ttgaatcgtc ccgcaaacat caaggtttcc taatgtcttt tgattactta catcaaaggt
     4201 caatcccttt acatcaggaa tattccgata gtagctgtat tcgtttgaag tagtcacatt
     4261 tccgtcgttt aactcagcat taaataatgg gtgtttgggt gaataaatta cttcattaat
     4321 agtcatcttt tccctccttg ctaaaaaaac tgcagcctag tagctgcagc ttcctactat
     4381 gcgattccaa gtaatgacaa taaaattccc aagacgacca ttccaaaaat aattttgatg
     4441 acgccgactt ttttatctag taatttgaaa cagcccaatg ttaccaataa cggtacaatt
     4501 cctttaaaaa tactatcgag gatttcttgg acattcatta gttcttcgcc cttcattgaa
     4561 aagctcaaaa cggtattgaa tttgaccata ctgatcgtca ttgcgcctac catgatcaga
     4621 cccaagatac tagcagcttt tgtcagaatt tcaattaacc cgttcgcata cagcttttca
     4681 atatatttag agcctaatga atagcctaaa acgccgccat agtatcgcat caaataatta
     4741 ggtatattga acataactaa aaagataatt ggtgccaaaa tatttccggc attggctaag
     4801 ttgatcgcta gtcccgcggt aacgacacgc caaactcccc agaacaatga atcgccaatt
     4861 ccagccaatg gtcccatcaa actcgttttg atcgcgttga tactttccac gtcaaaacca
     4921 gctttttcag aattttcctt ctccatcgat gccgcaatcc ccatgacaaa ggacgcgcaa
     4981 ggaatggtcg tcgaaaaata ctgtgtatga cgttccagcg ccgcagctcg atcttcttca
     5041 cttttgtaga aatgattaat gaagggcatc atagaatagg cgaaaccacc gccgcccatc
     5101 gtcaccggat tcaccgaagc atacatggtc atcgagcgcc aaaacatctt ccgaaccaat
     5161 tttttctcat ctttagaaat tccatcagct aatttcattg aaagaaatcc tcctcttctt
     5221 ggtcaagcgt tgcatttaca gcagcatcgc tttgaactgc atgaacagat atatttttta
     5281 gttgtagatc tctttgggct ataacaattg caataatcgt tccaactgcg gcaattgcaa
     5341 tcaatggtaa ctcgagatac gccataacaa cggccccaaa gaaaaaccac acagcaatat
     5401 ttttactcca aagcatccgt aacagcatcg ccatcccaac tgccggcagc agatttccag
     5461 cgactgttaa tccgttcatg atgacctctg gaatattatc caagacgctt ttgacaacat
     5521 ctgctccaaa taaaacggca aagaatggaa taagagcata aagcgcatag tggacaaacc
     5581 acataacgaa gtgcagattg atgattcctt tttgatcacc agattttgca aatttgaaaa
     5641 atttagcagc catcggtcca catacaaaag aaaaaatcat attcattaac tgtaatccta
     5701 atactgctag tggaaagaca atgggaataa tcgcatccac gtttcctccc attttgattg
     5761 cgaacgctgt agccaaccca gcagccaaag aaggctccgc cgtactagag attccgatat
     5821 tgacgacacc cataaaaatg gcttctagtg acgccccgac aatcaacccc gtctgcaaat
     5881 cccccatcaa cgctcctgct aacggagcaa caacaatcgg acgactcagc atggacatcc
     5941 cgaacatttc atgtccagca actccaataa aaacggccaa agcgactaca aatgcatcat
     6001 acataatttt tctccttcct ttttctaaaa atcactcaaa atagagatag ctgaacgttt
     6061 ttccatcgcc ggtgtaggct gaaaaacggt atctggataa agttcaacta attcttttaa
     6121 gtcttgaatt tctgacgtcg ttaacatcac ttcctttgat aaggttttct tatcttccga
     6181 tggcggtgtc attttcccag cattccccac gttcattaat tcgatttccg ttgggaaagc
     6241 tttcgcaact tttaccgcat ctttgacatt agggactaaa accatcaagc gcatgtcctt
     6301 cgcccgcgga tcttttaaca aatgaatcgc gtcatcaact gttttaataa tgactttgac
     6361 attcgatggt gcagccatct tcaatgtcat cttttgaata tcgttggtag ccgcctcatc
     6421 gcttgctact actaacccgg ttaaatttag actctttgtc caagtcatcg ctacttgtcc
     6481 atgaattaat cgttcatcaa ttcgcactgc tttaatcatt tttcttcctc ctaaaaaaaa
     6541 tcttcctctt catttactac atgtacctca tcaagaatgg tcatctttaa ttcctgttgg
     6601 catttattta ccagttcctg aatatcctcc tttgttaatt ccttggttgc caatactatt
     6661 tcgagcagaa ttggcagatt aaagccagac aaaatagtta cagcaatctc tttctccaaa
     6721 aaggttttgg taatcttctg attgacactt ccgccaaata aatcggtaaa aacaaaaatc
     6781 tgatcctgct cgttcacctg gtttacaaat acattcaact cctgttcaaa atcacactca
     6841 tccacataag cattaatcac cgtaatctct tgaaccttcc ccgtcagtaa ttgaatactg
     6901 ctaagaaagc ctgaagctaa gtgaccgtga gtcgctatta gaattttctt catatggtta
     6961 attcctttct ttctgttcac ttttatataa gcaattcctg tgccaacttt taaaaacatt
     7021 ggtttaatca cgttaattca cattaaaaaa agtgtctaac ctaaaaaaaa cagattagac
     7081 acttttttag acactttatt tttgaatata cggtcctaat aattcataga gcagcgaaag
     7141 ctcataagca ttgactttaa aattatattt catctccatt gattgaaaaa tacttctaga
     7201 aacataataa aattcatctt catctggttt caaatcataa ttcggttctt cagatttttc
     7261 tgtccgagcc aagaataaac gttccatcat caatgccgta tgcatataga gatttagttt
     7321 tacacggcca tttaaagtga aatgatagta attttcatat ttagaaatga ctgtttccac
     7381 ttcactcatc acgatcgttg ggttcagaaa gttcaaacgg tccgcaattc cttccaacga
     7441 aaagaatttt actagctcct gaatcaagcg gttaaattct gccggcttca tttcaggact
     7501 caaaatatgc tccaaattgg ctgctccatc accgtctaag atgtcataaa cattaatatt
     7561 aggaattgaa aaggaacgtg gaagatccga agtcgtgatg accaattttg tcttgtcaaa
     7621 ataagtttca tcattttggt cgattaattc acgtagttct ctgtagtcct tcgtgaaaac
     7681 ttcgatggat tcatccgata aatgatgacg aaaaacatct ttcaatttgt ccgaaatccc
     7741 caaacccgac atacaggaaa tgataatgtt gctactttga gaaaagcctt caaagtattg
     7801 aacattgatt gaatacttgt ctgctgcatc ctccgcaatc tgtttaaatg gcgcattcat
     7861 cgtcatcttc aagccaatat caagtgcaat ggaagtcgtc aaattattca gaagaagtaa
     7921 ctcccctttt aaatcgttct tgatctgtgt gtaaatttga tttaaagagc ccatatctac
     7981 gatcagcact agactatctg tattcacttg ccgtttcacg aaattccttg tcgattcgac
     8041 aatctctgac acattactct ctactggcat atccaacgct tcaaaaacat aattgccgca
     8101 taattgattc actactgctt gaatgctgct agccgtacta tctccgtgag caagcaacag
     8161 gcccttcagc tcgatggatt catctacata atcagataaa gtcacgacaa taagcagtct
     8221 tagaaagttc tcgtcctttt ctgagagcgg tggtaaatgc tccaagaact gattacataa
     8281 gtaagtcgtc cgaggtagat ttgcagctaa tgtacgattt atcccggtta agtctgtctt
     8341 atagcttggg gaaatgacat aggatctgct aataatttct gcaacaggtc cactcgtcgt
     8401 caatccgtac gtttttccta aaacggatac ccagaccttt ctaaacatct gaatcatcaa
     8461 ttttctactc attgataaaa aagaatcctt atcaatgtga tgcaccaaat gctgtacttc
     8521 attctttatc gaagaaaaat tttcattgaa attaccttca tttatcagct gagtaatttt
     8581 ttccaaaatc gaatcataat caaacggttt gattcgatcc gtttttatct tttgattttt
     8641 ctttatcaat aaactagcaa tcggcaagct ttctacttcg gattccagca tatttaactg
     8701 aatcaagatc tcatttttcc caatcgttaa tggctcttcc cgtgttcgac tgtacacatc
     8761 cgcacaactg attttaataa tattcttcag cttaccaata ttcccactaa agggatgaga
     8821 taacattaga gacaaagctt cttttgaaac aataatatct ttttgtaaaa tatccgcttc
     8881 attttgaaaa aataattgaa tcagctctaa ccgttctcga atgggacgat cttcaaaagt
     8941 tggcaacata acactgattt gaatacgtcg atcaaaggtg tctaataaat aatcttcact
     9001 tttttccgtc gtggcgaaga taaaacgaac cttagcagaa tgccagttta cattttcacc
     9061 gactggacga taatttccag tatcaataaa aacaaagagc ttttcttgat tttctttcga
     9121 caaacgatgg acctcatcga gaaaaagata gccattgttg gctgttgcca ataacccctc
     9181 tgtatccttc tcagcccccg taaaggcccc tttcttgtat ccaaacagtg ttgccgaaag
     9241 cagttcggga ttgtccgcat aatcggcaca atttaaaata gcaaatggtg catcttcttc
     9301 aataatttgc tcgtggatag cgtattcaaa aatttttgtg gctaaaaaac tttttccaac
     9361 ccccgtggct ccatttatta atacactgag tccgttaggc ggatatttga cagctgcaat
     9421 acatttttga atgacctctc tcagacttcc gtcatgaccc accacagaat gaaaggcttc
     9481 gtttatttgc tccgtattat ctacaaccgc atattcatct aaaggcttcc acaaaacagg
     9541 ctttcccggt aatttctcga cctttttatc ttccactaat ctcgtcaaat aatggctggc
     9601 attctgacga gagataccta gattcttcga aattttctcg gcagtgacag aagacttgtt
     9661 ccctgttagt cgtatcagct catcaaaaac tcgatcttta gcgctctttt tcattttccc
     9721 ctccagttca acgtgtctag aagcataata aactatcggc tacaatatgt ctattctata
     9781 tttctgttcc aattttactt tgaaacaaaa ataatcgagc ttaaactgat attagttata
     9841 aaactatagt tagattacca atcttcctct catacaataa acttctctca cctgcatcca
     9901 acctacttat gtgaaagaat aactgccgat tatttatctt ttttacagtt actttcgaac
     9961 ataaaaaaat caccttcaaa tactagaaaa actactattt gatggtggtt tagaacaatt
    10021 tccgttgatt cagtatcgtc tggtcccaca tcctctgtct cattaatcac aatagcatac
    10081 tccaaaggat aaagctggtt gatatctcaa tgcctttcac acctatcaat tttgtcacac
    10141 ttatcttttc ctaggttcta ttctatataa tctagaaaaa caaagaagga gacaccatga
    10201 aaaaattcat tattccttgt atatttcttt tcttgagtat tttgttattt tcaacttcca
    10261 tcctagtcgg ttcttacctc gattcaagcg gaatgctgat tgaaccagcg ttcttttgtg
    10321 ttccgttggg ctacttaaca ttaggacttg ctttctttac tgccttatac agtttcgtca
    10381 aacaaagatt attgaagaaa tactcggctt gatttgcacg taaatcctct gttgaagaaa
    10441 agttagtttt ttcaacaacg aaccttcgag tattaagaat ggatgttgat gtagacatca
    10501 aacgtctgca taaacattca ttttattcct ccattaccaa caattattcc caatcgccaa
    10561 aagaagcttt tttcggaatt accttctcca ttttttcacc tgctggttat attgtctttt
    10621 gtaataccga tagtcctctt caatcatttt caatatgcga aaagaaccaa ttttaacatg
    10681 gcactcaaat cacaattcct agggcggcca aaaagatacg gctcgttgat ttcaagggtt
    10741 tcaacgagtt cattgacaaa cccagcggtg ctatcttctt ttggaataag aagttgtgat
    10801 atctaaagtg agttgattca tgttataatt tgtatgcatg attttgatcc tttcaagggt
    10861 tgaatgattt agagtgcgag tatcctctcg tactctttta ttttacctaa aaagctccag
    10921 aaatgacgaa tgactcgtca tttctagagc ttttttcaga gaattatgtc acaatctctt
    10981 tttgttatat tgatattct
//
LOCUS       DACGLH010000092        10936 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.097, whole genome shotgun sequence.
ACCESSION   DACGLH010000092 DACGLH010000000
VERSION     DACGLH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.097"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(68..1624)
                     /gene="rny"
                     /locus_tag="IXT21_002491"
     CDS             complement(68..1624)
                     /gene="rny"
                     /locus_tag="IXT21_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ4869860.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(2035..3084)
                     /gene="recA"
                     /locus_tag="IXT21_002492"
     CDS             complement(2035..3084)
                     /gene="recA"
                     /locus_tag="IXT21_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ4869861.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3193..4434)
                     /locus_tag="IXT21_002493"
     CDS             complement(3193..4434)
                     /locus_tag="IXT21_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ4869862.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4674..5252)
                     /gene="pgsA"
                     /locus_tag="IXT21_002494"
     CDS             complement(4674..5252)
                     /gene="pgsA"
                     /locus_tag="IXT21_002494"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ4869863.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5461..6345)
                     /locus_tag="IXT21_002495"
     CDS             complement(5461..6345)
                     /locus_tag="IXT21_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4869864.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(7123..8409)
                     /locus_tag="IXT21_002496"
     CDS             complement(7123..8409)
                     /locus_tag="IXT21_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ4869865.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8402..9670)
                     /locus_tag="IXT21_002497"
     CDS             complement(8402..9670)
                     /locus_tag="IXT21_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ4869866.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9922..10383
                     /gene="mscL"
                     /locus_tag="IXT21_002498"
     CDS             9922..10383
                     /gene="mscL"
                     /locus_tag="IXT21_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ4869867.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10442..10888)
                     /locus_tag="IXT21_002499"
     CDS             complement(10442..10888)
                     /locus_tag="IXT21_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869868.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         2964 a         2383 c         1931 g         3658 t
ORIGIN      
        1 ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc ttcttttgta
       61 ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt actttgatgt
      121 gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact aaacgaacag
      181 cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt ccagcttgaa
      241 ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga cttcttaaac
      301 gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct gagagcgcat
      361 ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca tgatgagaag
      421 cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc gcaccgatct
      481 caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg agcaacccgg
      541 cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca gctaattttg
      601 caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat cgcagacgtc
      661 cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac gctgcctctt
      721 ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc atttcttcga
      781 tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc cgtgcgattt
      841 ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca tcgatgatta
      901 aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt ccaatgatac
      961 gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct gaaacttgat
     1021 ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga tctgcttctt
     1081 ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc agctcttctt
     1141 cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca attttttcta
     1201 gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct tcaattaatt
     1261 gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt ttttctaacg
     1321 agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct tgagatttta
     1381 attctaactt actttctctc agctcacttt caacttcaga acggtatttc tgattttctt
     1441 ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt gcactatcga
     1501 tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga
     1561 ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga atgttgaata
     1621 ccataaaatc cacctccata ctatcttttt catttttatc atctatacag cttctatgat
     1681 aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa aacatgcaca
     1741 actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag aaacggcttt
     1801 ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat ataggtaaac
     1861 ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga tatttttatt
     1921 ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata aaaaaacggc
     1981 aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata atttttattc
     2041 ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga cttcttcacc
     2101 aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag gatgattagc
     2161 catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat aagaatacca
     2221 agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt ctccttcttg
     2281 tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag gtgctacttt
     2341 gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt gtttcaattg
     2401 ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg
     2461 agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt tgatgaaaat
     2521 agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt gagacatcaa
     2581 acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg ctcgtggaac
     2641 taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata ctaaagcatc
     2701 agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt
     2761 cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa tgaaagcggc
     2821 cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg ttttacctga
     2881 actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga cacctaaagc
     2941 gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga ttttttcgcc
     3001 caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg cagcatctaa
     3061 ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa atcagagcaa
     3121 catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa
     3181 tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc cttgcataac
     3241 cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg aaaccacgcc
     3301 gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta acgtgtccgg
     3361 tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac gtgcttgaat
     3421 ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata gctccggatc
     3481 aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc ctggaaatac
     3541 ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg tcaaactttc
     3601 cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt ttgctagcga
     3661 attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt tttcttctaa
     3721 tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt
     3781 cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct caatcagatc
     3841 ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa ctttagaaat
     3901 tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt ccacaaacat
     3961 tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat ctgtttgata
     4021 aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt cgataatctg
     4081 ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat atgccaataa
     4141 ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg cagttgcttc
     4201 ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga gttcacttct
     4261 tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta caactgtttg
     4321 ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg cattcgtatt
     4381 cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt tcattacttc
     4441 ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa agagggcaag
     4501 gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt tctcctgcca
     4561 aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt tgaaattatc
     4621 ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga tacttacatt
     4681 gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata gatagtaaag
     4741 atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct gaatgggata
     4801 ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac ttttcccggc
     4861 caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc tgtgacggct
     4921 aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg gtcaaccaac
     4981 atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa
     5041 ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt gatactagct
     5101 aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc tcctacgtta
     5161 aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat catacaaatt
     5221 ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt ccctattcta
     5281 cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa tttattcaaa
     5341 ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc gacacagatt
     5401 tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta atttatacga
     5461 tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt tttcccctgg
     5521 attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg caccaatcgt
     5581 gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat ttgctgccag
     5641 tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac gattgacagc
     5701 agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga tggcactgcc
     5761 agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg ttgttgattc
     5821 tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact cctcgactga
     5881 tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt gacgatcttg
     5941 ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta ataaaatgac
     6001 aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg cttttctaga
     6061 agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt tgcttttccc
     6121 atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt attgccgaat
     6181 aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc
     6241 caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata tttttttatt
     6301 caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt catcttctcc
     6361 agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg tttgggtgct
     6421 gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc ctgccaactc
     6481 ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa cttgttcctt
     6541 gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt caatcagctg
     6601 ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc gtatgtttct
     6661 ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct
     6721 tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt tttacttggc
     6781 tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac ggtgttccag
     6841 aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca attttcgtaa
     6901 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat tacttcgttt
     6961 gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt ttctcactaa
     7021 tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt cctactacaa
     7081 gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg gtctcatgta
     7141 aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta cgtctttcat
     7201 ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa ataaactttg
     7261 cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt gtccaagcat
     7321 tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag cgaatccaag
     7381 aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc
     7441 cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat cgataattcc
     7501 ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt ggaacaacag
     7561 attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg tgtctaatcc
     7621 tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac tttgtttgat
     7681 gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag ggaaattttt
     7741 tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct taccaactac
     7801 aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca agtcttgagc
     7861 tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg
     7921 atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt catacatttg
     7981 gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga agtaaggtgc
     8041 ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg tagaaaacag
     8101 atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt tcccaaataa
     8161 ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat gggcaattcc
     8221 atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat cgatcgaacc
     8281 ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca acaaatacac
     8341 ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct catattctgt
     8401 tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg atttgctcag
     8461 ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat tcttcatcag
     8521 ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag
     8581 agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac agttccaact
     8641 ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga agtacttgat
     8701 ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac gtatccacgc
     8761 tactagaggc ataataagct agactttctt tctctctgac attcatgaaa agtttagagt
     8821 gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt
     8881 aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc tcacgtactt
     8941 gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga taaactgttt
     9001 cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga tcaacatcgc
     9061 cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag gacgctagcg
     9121 aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc ttttgttccg
     9181 ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt ttatcttcct
     9241 tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa
     9301 actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct accgcttgag
     9361 aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct gcgtttagcc
     9421 aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa agctctgcaa
     9481 gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc aacatgctgc
     9541 tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa cggataaaaa
     9601 tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct tctgccaatt
     9661 gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc ttttaatggg
     9721 tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg caattcgccg
     9781 ttttttctaa atgatgttac actttatagt atagcataga tgttttttat caaaagaaac
     9841 agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac taaagacaaa
     9901 taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat gcgtggagat
     9961 gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa
    10021 gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat cacaggtact
    10081 tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg cgtcacattt
    10141 gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt tgtcttattc
    10201 ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa
    10261 gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct tcttgccaaa
    10321 caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga tacttctaaa
    10381 taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt tttttagatt
    10441 cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct tccaaataca
    10501 catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc
    10561 cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa
    10621 tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt ccaaaatgga
    10681 tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca gtatcttgaa
    10741 aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga cactgttgtt
    10801 ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct gttgacaatt
    10861 cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt ttattcttag
    10921 cataccgatt cgcaca
//
LOCUS       DACGLH010000093        10510 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.098, whole genome shotgun sequence.
ACCESSION   DACGLH010000093 DACGLH010000000
VERSION     DACGLH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.098"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IXT21_002500"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IXT21_002500"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ4869869.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IXT21_002501"
     CDS             1085..2005
                     /locus_tag="IXT21_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4869870.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IXT21_002502"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IXT21_002502"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ4869871.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IXT21_002503"
     CDS             3311..4789
                     /locus_tag="IXT21_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4869872.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IXT21_002504"
     CDS             5066..7126
                     /locus_tag="IXT21_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869873.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEVHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IXT21_002505"
     CDS             7209..7778
                     /locus_tag="IXT21_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869874.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IXT21_002506"
     CDS             complement(7953..8759)
                     /locus_tag="IXT21_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869875.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IXT21_002507"
     CDS             8897..10411
                     /locus_tag="IXT21_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ4869876.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3726 a         1751 c         2071 g         2962 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagttcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DACGLH010000094        10488 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.099, whole genome shotgun sequence.
ACCESSION   DACGLH010000094 DACGLH010000000
VERSION     DACGLH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.099"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..357)
                     /locus_tag="IXT21_002508"
     CDS             complement(<1..357)
                     /locus_tag="IXT21_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ4869877.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
     gene            complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IXT21_002509"
     CDS             complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IXT21_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ4869878.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IXT21_002510"
     CDS             complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IXT21_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ4869879.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IXT21_002511"
     CDS             complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IXT21_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ4869880.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2237..2869
                     /locus_tag="IXT21_002512"
     CDS             2237..2869
                     /locus_tag="IXT21_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ4869881.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2871..3776)
                     /locus_tag="IXT21_002513"
     CDS             complement(2871..3776)
                     /locus_tag="IXT21_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ4869882.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3861..4514)
                     /locus_tag="IXT21_002514"
     CDS             complement(3861..4514)
                     /locus_tag="IXT21_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4869883.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4836..5780
                     /gene="mvk"
                     /locus_tag="IXT21_002515"
     CDS             4836..5780
                     /gene="mvk"
                     /locus_tag="IXT21_002515"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ4869884.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5773..6750
                     /gene="mvaD"
                     /locus_tag="IXT21_002516"
     CDS             5773..6750
                     /gene="mvaD"
                     /locus_tag="IXT21_002516"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ4869885.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6763..7848
                     /locus_tag="IXT21_002517"
     CDS             6763..7848
                     /locus_tag="IXT21_002517"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ4869886.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7845..8909
                     /locus_tag="IXT21_002518"
     CDS             7845..8909
                     /locus_tag="IXT21_002518"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ4869887.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9158..9961)
                     /locus_tag="IXT21_002519"
     CDS             complement(9158..9961)
                     /locus_tag="IXT21_002519"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ4869888.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="IXT21_002520"
                     /pseudo
     CDS             complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="IXT21_002520"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10268..>10488)
                     /locus_tag="IXT21_002521"
     CDS             complement(10268..>10488)
                     /locus_tag="IXT21_002521"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ4869889.1"
                     /translation="ALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVI
                     IIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3100 a         2219 c         2008 g         3161 t
ORIGIN      
        1 acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg gtccgattcc
       61 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
      121 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
      181 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
      241 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
      301 cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
      361 gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg
      421 agataactaa caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt
      481 tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt
      541 tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgttattt
      601 tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga
      661 aagaacataa cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa
      721 agcttctttt gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc
      781 agctaacatt ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga
      841 gtagctcaag ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa
      901 gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc
      961 ttttgctgtt ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac
     1021 tcttttacga cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc
     1081 ctaataaaaa taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt
     1141 gttggcgaat acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc
     1201 gttgtttttt tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc
     1261 cgcctttacc agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca
     1321 tgactatatt tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt
     1381 tagtcattca aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc
     1441 gtccattttc gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt
     1501 caagactttt tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc
     1561 tttcacttta tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc
     1621 attcaagtcg atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt
     1681 acgcgcttcg cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg
     1741 cgcaactggt ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc
     1801 aatattcaat gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa
     1861 tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca
     1921 cctccaaata ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt
     1981 tctcttacag aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag
     2041 tgacattatc aactaaggcg agaagcgggt gcttctgctt ttatttctca accattatag
     2101 tatagcaaaa actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa
     2161 aatgattaga ttagcaggct ttctagcagt gattcccgct tctttgctat actaaagaaa
     2221 aacgaggtga acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac
     2281 aaagggagag ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggagg
     2341 taaccaaagc tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact
     2401 tacgatgcaa aaaacaggtt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg
     2461 tgatttttcc aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg
     2521 taaattaaca ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg
     2581 tattgcttcg atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg
     2641 tcaattagaa gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa
     2701 tcagttaacc ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa
     2761 tttcttcaca acaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaatcaaga
     2821 gcaagcactt gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc
     2881 cgccgccaca gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt
     2941 tcccatccga aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc
     3001 acaatagttg gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc
     3061 aagttgttat tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct
     3121 aagttcgtca tttcaactgc ttgtcgtgct tcgatcagtg catcgtccaa ctcttctttt
     3181 tccttttctg acaacttgcg ataaatagcc agtgccttga tttgttctaa aagtactaca
     3241 ttttgttttg ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc
     3301 agattcttgt tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc
     3361 acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa
     3421 ctgtatacac tcatgtctgg catccgttcc aaaagcgatc gaattagtcg aatcacgtac
     3481 tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga
     3541 ctagtttcat aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac
     3601 gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc
     3661 agcatctcgt cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt
     3721 tgctgtacat tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc
     3781 tatcctcctg acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc
     3841 tttatttgag caactgtaaa tcaaatggat actttggttt ggcgaatctc tgctaatttt
     3901 tcttcgatca ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg
     3961 tctccatcga tttggatttt tggactttct ttgtattctt cataccattg atcataacgt
     4021 ctgttcaatt ctttgtaata atcgtataga cttgggtcaa aggacaattg ttcatacgaa
     4081 cgaccacgtt tttcgatacg ctctaacatc gtatcaaaag atacacgaat atgaactaat
     4141 aaatccggat gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt
     4201 tcatcataca cacaaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa
     4261 agcagtgaat cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt
     4321 ttgatgcttg caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca
     4381 gggtttgcat aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga
     4441 ctatcaaagt gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct
     4501 gctaaaacga tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac
     4561 tccaaccttt tttaaatata cccccaatat ctagtagtgt caattgtatt ttttctagtc
     4621 aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta
     4681 ttcttagttt tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc
     4741 cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc
     4801 ttaaaaatca cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat
     4861 caagcggaaa gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg
     4921 cctttccttt ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg
     4981 atcaactggt ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa
     5041 atatcaaaaa attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac
     5101 tgaccattga aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca
     5161 cagcagtcac tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat
     5221 tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa tcctagtgga atcgatgcag
     5281 ccgctactag cagcttgcag ccgatttatt ttacaaaagg gcatcctttt gactactttt
     5341 ctttgaacat cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag
     5401 aagccgtcaa agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga
     5461 tccaaaaatt aggatacttg acgaagcaag caaaacaagc aatcattgag aattcaccag
     5521 aaacgttggc acagacaatg gatgaatcac agtcacttct ggaaaagctg acaatcagca
     5581 atgattttct taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat
     5641 taactggcgg tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc
     5701 aagaaatcag tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag
     5761 gagtacatac ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa
     5821 tattggggta agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca
     5881 ttggatgcat tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa
     5941 ttttatctag atggcacctt acaagacgaa aaagcaacaa aaaaagtcag tcagtttctt
     6001 gacctttttc gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc
     6061 gttcctaccg cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggagct
     6121 tgcaatactg ctcttaagct tggattagac gatctctctc tttcaagatt tgctcgacgc
     6181 gggtctggtt cagcttgccg aagtattttc ggtggtttcg ttgaatggga aaaaggccat
     6241 gacgacttaa gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca
     6301 atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt
     6361 cgaacagtcg aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta
     6421 tatcaattga aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa
     6481 agaaacggac taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg
     6541 tctcctgaca gcttaaaagc gatggatgcg gtgcggcaat tgagaaaaaa aggcattcca
     6601 tgctacttta cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta
     6661 tctgaggtac aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcccat
     6721 gctggtccag gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca
     6781 ccaggcaaac tctatattgc cggagaatat gcagttgttg aaacaggaca tccagcagtt
     6841 atcgctgcag tcgatcaatt cgtaacagtc actgtagaat ccgcacgaaa agtcggaagt
     6901 atccaatctg cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta
     6961 gttttagata ttcgggaaaa tccttttcat tatatccttg ctgctattcg tttgactgaa
     7021 aagtatgcac aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag
     7081 ttagacagtt caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca
     7141 acggtcaaag ccttgaatgt tttttacgcg ttgaatttat ctcaattgga gattttcaag
     7201 attgcagcac tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct
     7261 agctgttatg gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa
     7321 gaaactcagc attctatcag tgagttactt gccctggatt ggccaggtct atccattgag
     7381 ccattgattg ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct
     7441 acttctgatt tggtcgatca agttcaccgt tcgagagaag acaaaatggt ggcttatcag
     7501 cttttcttaa aaaacagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat
     7561 aatgtaacgt tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct
     7621 gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa
     7681 cagtatgaag gagccgcaaa atcttctggt gcaggtggtg gcgattgcgg aatcgtaatt
     7741 gttgaccaga aatctggcat ccttccttta atgagtgcat gggaaaaagc agaaatcact
     7801 ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg
     7861 aacatgtttc actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac
     7921 ggatcatcca taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg
     7981 cagttgggtt tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa
     8041 aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg
     8101 ccactggttc agtcagcgct gccttgaaag acccttcttt agccgatact tatacgatta
     8161 tgcgacaaga atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg
     8221 aaagagccca agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg
     8281 cacctcaaga gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta
     8341 ttcaagagac tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca
     8401 tgacaagaga aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg
     8461 gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga
     8521 gttatttagc tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag
     8581 acacttctat ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag
     8641 ccctttgtct cggcgcaaat gcagtgggta cttcaggaac cgtactgacg catctgatga
     8701 atcacggcgt agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc
     8761 tttatactat ggtcggcgca acgaataccg cggccctaca ccaacaatca ctgatctttt
     8821 ccggaccagt gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac
     8881 gcacagaaaa gagcgtggga caaaagtaaa gaacactttt atcccacgct ctaaacacgt
     8941 ataaacggcg gaagcaaaag caactccttc ggaaataagc tgaaattcac aaaaatttga
     9001 aaagcaattt tcgtgaattc cttcttattt cttggagtta aacgcttctg tcccagcctc
     9061 ttttttcgaa taaatgatgt ttaatcagct gtccttcggt attcgttcaa gccgctgatt
     9121 tcacaacctc tccactaaac ggtgtactaa agctctttta aggttgtttt ccgatatagg
     9181 caagtatgcc tccgtccaca tacaagacat ggccgttaac gaaatcagat gcgctagaag
     9241 ccaagaagac agatggtcca gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac
     9301 gtccaacgat aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg
     9361 gagcagtttg cggtgtagca atataacctg gtccaattcc attacattgg atattgtatt
     9421 gaccgtattc agaagcgatg tttttcgtca gcatttttaa tccgcctttt gccgcagcat
     9481 aagcactgac cgtttcgcgt cctaattcac tcatcatgga gcagatattg atgattttac
     9541 cgccgccttt ttcgatcata tcccgaataa ctgcttttga aacgatgaat ggggcattta
     9601 aatccacgtc gattacttgg cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt
     9661 tgatgatccc tgcattgttg actagaatat caactgaacc tacatcttct ttgatctgtt
     9721 gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca gacataacct tttgcatcga
     9781 tgcctgcttc tttataatgc gctatacctt catcaactga ttcttgattc aagttattaa
     9841 atacgatttt tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca ataccataga
     9901 cagcaccggt gactaatgct acttttccat ctaaacgaaa catatccata ctaaattcca
     9961 tcattttctc tcccttatgc ttattttaat tgatccattt gcaccatatc catgtctgta
    10021 taggtgatat tttctccgca cattgcccag atgaatgagt aattgcttgt acctacacct
    10081 gaatggatcg accagcttgg cgaaataatg gcttgttcat tgtctacgac taaatgtttt
    10141 gtttcatctg gtttgcccat catatggaac acacgtgtat cttcattggc ataatcaaaa
    10201 tagacataag cttccatacg acgttcatgt gtatgacaag gcatcgtatt ccaagcactt
    10261 cctggttcta aaatcgtata acccatttat aaatgacagc tatcgctgaa gcttctactg
    10321 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
    10381 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
    10441 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgccc
//
LOCUS       DACGLH010000095        10487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.100, whole genome shotgun sequence.
ACCESSION   DACGLH010000095 DACGLH010000000
VERSION     DACGLH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.100"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(251..1462)
                     /locus_tag="IXT21_002522"
     CDS             complement(251..1462)
                     /locus_tag="IXT21_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4869890.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(1580..2215)
                     /locus_tag="IXT21_002523"
     CDS             complement(1580..2215)
                     /locus_tag="IXT21_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ4869891.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2298..2720)
                     /locus_tag="IXT21_002524"
     CDS             complement(2298..2720)
                     /locus_tag="IXT21_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ4869892.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2738..3058)
                     /locus_tag="IXT21_002525"
     CDS             complement(2738..3058)
                     /locus_tag="IXT21_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4869893.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3351..3542
                     /locus_tag="IXT21_002526"
     CDS             3351..3542
                     /locus_tag="IXT21_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869894.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            3556..3807
                     /locus_tag="IXT21_002527"
     CDS             3556..3807
                     /locus_tag="IXT21_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4869895.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            4019..4336
                     /locus_tag="IXT21_002528"
     CDS             4019..4336
                     /locus_tag="IXT21_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869896.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            4269..4748
                     /locus_tag="IXT21_002529"
     CDS             4269..4748
                     /locus_tag="IXT21_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAQ4869897.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPMPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            4769..5509
                     /locus_tag="IXT21_002530"
     CDS             4769..5509
                     /locus_tag="IXT21_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4869898.1"
                     /translation="MVKKIKAEDLSVEKGTYMIYANPGMGKTYSLGFLPGKSLVLDVD
                     GSSSTLAKHPNKENIEVWELDSSDIWQEWLDTISDLVANKSSYEKQFDNICVDNISEL
                     FKAQLEDLGKKGKNSGVPSQADYQRTDFMNLRGLRALNNLDCRIVLTAWETTDTYTEP
                     NGQFFTRSMPDIRSKILNNFLGLCDVVGRLVIKKDDDGNETRGLILKPTSNVYAKNRL
                     DERSGCLVEQLVVRAGGEPNVSTPTVSD"
     gene            5481..7004
                     /locus_tag="IXT21_002531"
     CDS             5481..7004
                     /locus_tag="IXT21_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4869899.1"
                     /translation="MYQLRPYQIKLVQEARKHLSQGKKGVLIQSPPGSGKSVVIAEIV
                     RLATRKGGTVLFLAHRRELLDNIRETLEQNEVDLSKVIILSAVMAKNRLNKLPKLSLI
                     ITDEGHHGKAKTYMDIYNHFKEIPRLGFTATPYRLNGEGFTDIYEEMVEGPSIQWLID
                     HHNLAPYRWYSIPLIDRSKVDFKNMSREAESSARLFESDATIQGDIVKNYKKYADGQQ
                     AIVYAPTIQVSKMIVKWFNDNGIYAVHADGKTPTKERDDIMANFKSKKITILSNVDLI
                     SEGFNVPDVGVIILCRPTQSIVLHLQQSMRGMRYRENKTSIVLDHVGNGANLGLPTDE
                     FEWSLSARKKKSNGSSSEAPRMTCSNCGQQFLLKSLLKIENKPHCPFCLQEIVSEEKE
                     NSVTFDEAVQMVELNAENAKLARLSRKKFSKKQSLELNYAIAKAKVSFEGKGNPLFKM
                     FGSLTAYQGQHYSIDQLEELSLIKDVSMESILRAYKWALEKLNSKQEEPEWVKNTFY"
     gene            7022..7528
                     /locus_tag="IXT21_002532"
     CDS             7022..7528
                     /locus_tag="IXT21_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAQ4869900.1"
                     /translation="MTAFKVDYNEAQDFGAVPDGDYEVVIFNVTEDASKGGTEFINFD
                     MVIRNDIKQPRQNSHLFHRVWKSKETGKYNRGMIMSLAKSFGLPDGKEYQSFENFLED
                     FAMRPAKVKVKNEQSEYNGKTYDNTNIKKFETTKFPELQHQWKKNNAEKSVNSSPAFD
                     ISDNDLPF"
     gene            7528..9864
                     /locus_tag="IXT21_002533"
     CDS             7528..9864
                     /locus_tag="IXT21_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ4869901.1"
                     /translation="MNNYERIPLELRELNQWGIYKRSWNEQRGKWSKKPYDPFTGELG
                     SSTNESKWSDFKTALSAVSTFNADGLAFYFKPPYIGIDLDDIGDDLERYLQGDVENNL
                     VYVFMNSTKTYSEISMSGKGIHIIGKAAIPGERRRKGNVEMYTEGRFFAITGNFFGNN
                     EEINEIPEIQMNFLYKRYLENETVIKQDFSKSNWSDGNDLSVNEIIQTAVNSSTGNRF
                     RLFMDGGWEKIYDSQSEADLAFANDLAFWTAGDFQKMDEIFRMSSLFRDKYDQKRGKT
                     TYGIGLLNKAISENTNHYTGKKTADDYFLSIPGITVDETKPTKFYSYDDTGNAERYLD
                     LFGSFTKYSYVNKCWYFYNGKNWEQDNIGAVRKWVDQTIEIFKNEPVSIPKDATEDEE
                     KAYIEAKEKHLRRSRNNAGKEAMTRELKHQVAILPDEFDSDDMLFNAQNGYLDLSNGI
                     LYEHDISKMFTRISNAEYTDKSDCPRWKLFLEQIFDNNTELIRYIQKAVGYSMSASIR
                     EQVMFILFGNGRNGKSVFLDIISEIMGSYAMGMQASTLMVKQGGSSGHNEDIARLNGA
                     RLVTSSEPNEGVRMDEGLIKQLTGGDKVSASYKGGHMFDYKPKYKIWLATNHKPIIRG
                     NDDGIWRRLPLIPFTVQIPLDKVDKNLKEKLMRELPGIFNWAVEGCLMWQREGLNPPA
                     DIQRATMEYRKEMDIIGAFIDECCETGPGYSIGATDLFKAYDKWARDMNEHPFSQTQF
                     GKKAADRFEKSKSKGKVVYRGIDLKKEFREFNVLVPGL"
     gene            10179..>10487
                     /locus_tag="IXT21_002534"
     CDS             10179..>10487
                     /locus_tag="IXT21_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAQ4869902.1"
                     /translation="MTAEIEIQNAIRRELPKYGHFVYRGNVGKVKTIDGRWFDTGLPK
                     GWPDLFGWTKEGKFFAIEVKNEKGKLRPDQVKFGDFLQKQPVLYGVARSVEEALKIVE
                     E"
BASE COUNT         3574 a         1662 c         2017 g         3234 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttatata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta atagtttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ctaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gtaaagttcc gtcttgtttt gtatcaaata
      541 taaaatagtg tcttaatttc tttatattta atttccaacg aattttttta ttctctttga
      601 tcaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttcttccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggttttat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaataaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctattcgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtaccctaat acagctctta gtctatatgt ttcattgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctattgtgta ggtttgtttt ttcttagaac acgctcgctt tggtcggtgg
     1561 ggcgtgtttt ttatactaat tagttatgaa gagcaattga aactgtctct gttccaaagt
     1621 aacctgtttg tgcttgcaat acattatttt gtgcatttgc ttgggcttca gagatatcaa
     1681 aaactatttt accagtttgt tgcatatcag gatttaagct ttccataaag aaagagttag
     1741 tgatattacc attttcatct tgatttgccg acatagatgc agccgaatca gcttcgaaag
     1801 tttttccatc tgcttttaat ttgaagaatg agctatcaac agtcacagcc ttatcacctg
     1861 cattttttac agataagtca actactaaga aagtgtcttt tgcgttagta ggaagtacag
     1921 atgggccaac ttgttttact acttcaacgg agtttacttt gtattccatt ttccctacag
     1981 caacgtcatc accgatttta taagtctttt cttcttttgt tgtagtttct tttgtagttt
     2041 ctttattagt acttgttgat gttgcagtag ctgaatcttt gttttcaccg ccacttagag
     2101 caccaccaat accaaaaaac aaaagtacta ttaataccca aaaccatacc cgtttataaa
     2161 acggtttttt aaccttatac attttcccat cttgacccat aacttttttt gccatttaaa
     2221 tatccctcac ttcttgttat aatatatttg tgatctcaga aatgaggtat gagtccgtgt
     2281 tgcagcacgg gcttttttta ctgtgcataa gagtattttt tcttgaaata tgactggcaa
     2341 acattaaaac attctgttct taacttatta ttgatagagt agaattccat gaaattttct
     2401 aatttgaact gagattcatc agtcaattcg ttctcaataa agatatttag taaaatcata
     2461 attgcgattc tatcagcttc agcttcgaat tttgaatgaa aagttgtaga gttatcgtac
     2521 agtactgaat attcaaaatg tgaagcaatg aaatgaccga gctcatgggc taaatgaaaa
     2581 gcttcagaac tgtcttcgtg tagtttttca ttcaaaaata ctattcttgg ttttggataa
     2641 taaaaacctg gttcttccat ttccatatag attaacttta aattatactc actcagcatt
     2701 tctttcaact ttaaatacat acaacccatc actccaacta ttcattttcc tctaaagctt
     2761 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
     2821 atacagtatc atcttctgat aaatccacat gtttaggcgt ttctttttct tctctaccta
     2881 gaagatagtc tacagagaca ttgaaatagt cagctacctt ttgcaaatct cttgctttgg
     2941 gttctgttgt tttccatcga tagaaaagat tttcgctaaa tcctaattct aaagcgactt
     3001 ctttcatgct tttatctctt tggctagcta atgattttat cctttcaaac agtgtcataa
     3061 taatgttctc caatcctaaa caaataaaag tacacaaaat gtaaattata ctgttgacat
     3121 aagtataagt ttgtattatt atttgtttgt aagttaattt gatagaaaaa aagcaaacta
     3181 aaaatacacc ttatagcatt aagtttggcg accgattgca taataaaggg tttgttgtag
     3241 gcttatttaa ctatgtttat atagtatacg attgtatact attagtcaat agtttatacg
     3301 ttttttctat cattttgact tacaaatcta tatgtaaggg gagtgaatag atgtcaaatc
     3361 tagataacgg ccgtgctgca atcaagaagt ttatgcgaga aaacggagta actatggaag
     3421 atttggctac tgcatatgga tacacacgtg ttcgaatgca gcaaattatt gatggccatt
     3481 ggagtggacc aaaagctaat aaaactatct tagaaatcat ccgtgattac agaattcgat
     3541 aggaggaatt aatcaatgga acaactagct ttagttaacc tctcagccct caaagcttct
     3601 ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc ggaatatttg
     3661 aaaaccacga cacgcacgct aactaaagat gccgaatctg gaaagattcc agcagcgaaa
     3721 gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa aggaggaaaa
     3781 acatgggcaa attcaacaga gcactagtat tcagcgcacc gctaatcatc tacgctttag
     3841 ggctttgggg aaatagacaa gcgttgatag gaacgattgt gtacatggtt tggattttta
     3901 tagggcttga tgaagctgag tacagagcga aaaaaagaca cgaacgccag caagcatgat
     3961 cgtgtcaagg aaataaacta tctttctata ttttaccaca gaaaggaatg aaccgtaaat
     4021 gctaattgca acggatacac tagaccgcat ctttttaaaa gacgaataca aactgcgcaa
     4081 tatagatgcg tcaggaattt tagtatttga tctttatgac aatggaaaaa ttggtatcta
     4141 tcaagcaagt gatattgaag aaacaaacct tgctttcgag caaattgatg attctgtgga
     4201 attggattta gatgaggcaa tcctagcttt tgaacaaatt gcaaaattat taaaggaggc
     4261 acaaaagaat ggcaactctt taccaactca gcgagtcata tatcaaagtc ctggaactgg
     4321 cagaacaatt ggatgaagaa attcttcgtg atactctcga ttcgattaat gaagcgatcg
     4381 agtataaagc agaaaactta gcaaaaatag ttaaagaagt agaagggaaa gcagagttaa
     4441 tcgatagtga aattaaacgt ctacaggaac gtaagacatc gcttttgaac aatgctaaga
     4501 gtatcaaaca ctatttacaa gaggaaatgg aaaagactgg taaaacgaag attaaaggcg
     4561 aattattcaa cattggaatt caaaataatc cagtatcggt caatgtaatc aatgagaact
     4621 taattccaaa aggattcttt acccctatgc ctcccaaatt ggataaaaag caattgaagg
     4681 aggaactgaa gcatggagat attcctggtg ctgaactcgt ccaaacaaaa ggtttgagga
     4741 taagatgatg caagtggagg tatgagtaat ggtaaaaaaa ataaaagccg aagatctatc
     4801 agttgaaaaa ggaacttaca tgatttacgc aaatcctggt atgggaaaaa cttattcttt
     4861 aggatttctt ccaggtaaat cattggtatt agatgttgac ggatcatcat cgacattggc
     4921 aaagcatcct aacaaagaaa atattgaagt gtgggaatta gattcttctg atatttggca
     4981 agaatggctt gatactattt cagacttagt cgcaaataaa tcttcctacg aaaagcaatt
     5041 cgacaatatt tgtgtagata acatttctga attgttcaaa gcgcaattgg aagatttagg
     5101 caagaaagga aaaaatagtg gtgttccttc tcaagccgac tatcaaagaa cagattttat
     5161 gaatttgcga ggtcttagag cattgaataa tttagattgc cgaatagtcc tgactgcatg
     5221 ggaaacaaca gacacatata cagaacctaa tggacaattt tttacaagat caatgccaga
     5281 tataagatca aaaattttaa ataatttttt aggcttatgt gatgtagttg gcagacttgt
     5341 aataaaaaaa gatgacgatg gtaatgaaac aagaggctta atactaaagc ctactagtaa
     5401 cgtttatgca aaaaacagat tagatgaacg tagtgggtgt ttggtggaac aattggttgt
     5461 tagagcaggc ggtgaaccga atgtatcaac tccgaccgta tcagattaag ctagttcaag
     5521 aagccagaaa acatctatct caagggaaaa agggagtatt gatccaatcg ccaccaggaa
     5581 gtggcaaatc agttgtaatt gcagaaattg ttcgattagc aacaagaaaa ggtggcacag
     5641 tactatttct tgcccacagg agagagttac ttgataacat tcgagaaact cttgaacaaa
     5701 atgaagtaga tttatcaaaa gtcataatat tgtcagctgt aatggctaaa aacaggttaa
     5761 ataaattgcc aaagctgtct ctgataatta cggatgaagg gcatcatggt aaagcgaaaa
     5821 cttatatgga tatttataac cattttaaag aaattcctag actaggtttt actgctacgc
     5881 catacagact caacggagaa ggatttacag atatatacga agaaatggta gaaggaccat
     5941 ccatacagtg gttgattgat catcacaatc tagcgcctta tcgttggtac tctattcctt
     6001 tgattgatcg ttctaaagta gattttaaaa atatgtcacg tgaagctgaa agttctgcgc
     6061 gattattcga gtcagatgcc acaattcaag gtgacattgt taaaaattat aaaaaatatg
     6121 cggatggtca acaagcaatc gtttatgctc caacgattca ggtaagcaag atgattgtta
     6181 aatggtttaa cgacaatgga atatatgcgg ttcatgcaga tgggaaaaca cctaccaaag
     6241 aacgtgatga tattatggca aatttcaaat cgaaaaaaat cactatatta tcaaatgtcg
     6301 atttaatcag cgaaggattt aatgtaccag atgttggagt gattatcctt tgcaggccta
     6361 cgcaatcaat cgtcttacat ttacaacagt ctatgcgagg catgagatat cgagaaaata
     6421 agacttcgat tgtgttagat catgttggga acggagctaa cttaggttta cctactgatg
     6481 aatttgaatg gtcgttaagc gctaggaaaa agaagagtaa tggaagcagc agcgaagcgc
     6541 ctagaatgac ttgctcaaat tgtggacagc agtttcttct gaagagctta ttaaagatag
     6601 aaaataaacc acattgcccg ttctgcttac aagaaatagt aagtgaagaa aaagaaaatt
     6661 ccgttacttt tgatgaagcg gttcaaatgg tggaattgaa tgcagaaaat gccaagctag
     6721 cgcgactttc aagaaagaaa ttttcaaaaa aacaatcttt agaattaaat tatgcgattg
     6781 caaaagcaaa ggtaagtttt gaaggtaaag ggaatccgct ttttaaaatg tttggctcac
     6841 tcactgctta tcaaggacaa cattactcca tcgatcaatt agaagaatta tcgttgatca
     6901 aagatgtatc aatggaatca attttaagag cttataaatg ggctttggaa aaactcaatt
     6961 caaaacaaga agaacccgaa tgggtaaaaa atacatttta ttaaggaaga ggtaattaat
     7021 tatgacagca tttaaagtag attacaatga agcacaagat tttggagcag taccagacgg
     7081 agattatgag gtagttattt tcaacgttac ggaagatgcc tcaaaaggcg gcacagagtt
     7141 tattaatttt gatatggtca tcagaaatga tattaaacaa ccacgacaaa acagtcatct
     7201 tttccataga gtttggaaaa gtaaagaaac tggcaaatat aatcgtggga tgatcatgtc
     7261 gctagcaaaa tcatttggcc ttcctgatgg aaaggaatat caatcattcg aaaatttcct
     7321 tgaagatttt gctatgagac cagcgaaagt aaaagtaaag aacgaacaat cggagtacaa
     7381 cgggaaaacg tatgacaata caaatattaa aaaattcgaa accactaagt ttccagaact
     7441 ccaacatcaa tggaagaaaa acaacgctga aaaatctgta aattcctcac cagcatttga
     7501 tatttcagat aatgatcttc cattctaatg aataattacg agcgtattcc cttagagttg
     7561 cgtgagttaa atcagtgggg gatttataaa cgctcatgga acgaacaacg agggaaatgg
     7621 agtaagaaac cttatgatcc gtttactgga gaattaggga gcagtacaaa tgagagcaag
     7681 tggtctgatt tcaagaccgc tctctcagct gtttcaactt ttaatgcaga tggcctagct
     7741 ttttatttta aaccacctta tataggtatt gatttagatg atataggtga tgatttagaa
     7801 aggtatcttc aaggcgatgt agaaaataac ttagtttacg tttttatgaa ttctacaaaa
     7861 acatattcag aaatatcaat gagtggcaag ggaatccata ttataggtaa agcagcaatc
     7921 cctggagaaa gacgtagaaa agggaatgtc gaaatgtata ccgaaggtag gttctttgct
     7981 attacaggta acttctttgg taataatgaa gaaattaatg agatacctga aatccaaatg
     8041 aatttcttat acaagcgata tttagaaaat gaaacagtaa taaaacaaga tttttctaaa
     8101 agtaattggt cagatggaaa tgacttatct gtcaacgaga tcatacaaac agcggtgaat
     8161 tcttccacag gtaatcgttt tagattattc atggatggag gatgggaaaa aatatatgat
     8221 agccaatctg aagcagattt agcatttgca aatgatttag ctttttggac tgctggagat
     8281 tttcaaaaga tggatgaaat attccgaatg tcttcgttgt ttcgagataa atatgatcag
     8341 aaacgcggaa agacaactta tggaataggg cttttaaata aagccatatc tgagaatacc
     8401 aatcattata ctggcaaaaa aacagctgat gattattttc tttccatccc aggtatcact
     8461 gtggacgaaa ctaaaccgac taagttttat agctatgatg atacaggaaa tgcagaaaga
     8521 tatcttgatt tgtttggatc atttacaaaa tacagttacg taaataaatg ctggtatttt
     8581 tataacggta aaaactggga acaagacaat atcggtgccg ttcgaaaatg ggtagatcag
     8641 actatcgaga tattcaaaaa cgaacctgtt tcgataccta aagatgcgac tgaagatgaa
     8701 gaaaaagcct atattgaagc aaaagagaaa catttaagaa ggtctagaaa caatgctgga
     8761 aaagaagcta tgacacgtga gttgaaacac caagtagcga tacttcctga tgaatttgat
     8821 agtgatgata tgctgttcaa cgctcaaaac ggttatttag atctttctaa tggcatttta
     8881 tacgaacacg atatttctaa aatgttcact cgaatttcta acgctgagta tacggataaa
     8941 agtgattgtc cacgttggaa gctgtttttg gaacagatat ttgacaataa cactgaattg
     9001 attcgctata ttcaaaaagc tgttgggtat tctatgagcg catcgatcag agaacaagtc
     9061 atgttcatac ttttcggtaa tggaagaaat ggtaaatctg ttttcttaga tatcatttct
     9121 gaaataatgg gaagttatgc catgggaatg caggcatcaa cattgatggt taaacaaggt
     9181 ggtagcagcg gccataacga agatatcgca cgattaaacg gcgcacgtct agtaacttcc
     9241 tcggagccaa atgaaggcgt aagaatggat gaaggtttaa tcaaacaact aactggcgga
     9301 gataaagtgt cagcatctta taaaggaggc cacatgtttg actataaacc taaatataag
     9361 atttggcttg caactaatca taagccaatc atcagaggaa atgatgatgg tatttggcga
     9421 agattgccat taattccttt cactgtacaa attccattgg ataaagtgga taagaatttg
     9481 aaagaaaaat tgatgcgcga attgccagga atattcaatt gggcagtaga aggatgtttg
     9541 atgtggcaaa gagaagggtt gaatccaccg gccgatattc agagagctac aatggaatat
     9601 cgaaaagaaa tggatattat aggtgctttt atagatgaat gttgcgaaac aggacctggt
     9661 tattcaatcg gggcaacgga tctatttaaa gcatacgata aatgggcaag agatatgaat
     9721 gaacatccat tcagtcagac ccagtttggt aagaaagctg cggacaggtt tgagaaatca
     9781 aaatcaaaag gaaaagttgt ttatcgaggg attgatttaa aaaaagagtt tagagaattt
     9841 aacgtattag ttcctggatt gtgaaacaaa aaagtttcac aaatgtgtgt aggtagacag
     9901 ttgggtagat agtaagtaga tagtttttta caaactgtct acccgataaa acctttgggg
     9961 ctgtaaggct cattactgtt aggtagatag tagggatagt ttatatatat agtaaataaa
    10021 ataataaaaa taaggaaata tataaaaata cagaagcaac ctagaaataa ctgtctactg
    10081 tctacccgaa ctggttaata cttagagcca caagggatag agcgattata aactgtctac
    10141 ccatgtatat atactatcta cccgagcggg aggcgcttat gacagcagaa attgaaatac
    10201 aaaatgccat tcgaagagaa ttaccaaaat atggtcattt tgtttataga ggcaatgtgg
    10261 gcaaagtgaa gacgattgat ggcagatggt ttgatactgg attgcccaaa ggatggccag
    10321 atttatttgg atggactaaa gagggaaaat ttttcgccat tgaagtgaag aacgaaaaag
    10381 gaaagttgag accagaccaa gtgaagtttg gtgatttttt gcaaaagcaa ccagtcctat
    10441 atggtgttgc acgatcagtg gaagaagcat tgaaaattgt ggaggaa
//
LOCUS       DACGLH010000096        10266 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.101, whole genome shotgun sequence.
ACCESSION   DACGLH010000096 DACGLH010000000
VERSION     DACGLH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.101"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            147..377
                     /locus_tag="IXT21_002535"
     CDS             147..377
                     /locus_tag="IXT21_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ4869903.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            474..1775
                     /gene="murA"
                     /locus_tag="IXT21_002536"
     CDS             474..1775
                     /gene="murA"
                     /locus_tag="IXT21_002536"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ4869904.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1788..1964
                     /locus_tag="IXT21_002537"
     CDS             1788..1964
                     /locus_tag="IXT21_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ4869905.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2099..2395
                     /gene="yidD"
                     /locus_tag="IXT21_002538"
     CDS             2099..2395
                     /gene="yidD"
                     /locus_tag="IXT21_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ4869906.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2579..3046
                     /locus_tag="IXT21_002539"
     CDS             2579..3046
                     /locus_tag="IXT21_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869907.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3131..4015
                     /locus_tag="IXT21_002540"
     CDS             3131..4015
                     /locus_tag="IXT21_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4869908.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4132..4365
                     /locus_tag="IXT21_002541"
     CDS             4132..4365
                     /locus_tag="IXT21_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ4869909.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4453..4533
                     /locus_tag="IXT21_002542"
     tRNA            4453..4533
                     /locus_tag="IXT21_002542"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4487..4489,aa:Tyr,seq:gta)
     gene            4540..4611
                     /locus_tag="IXT21_002543"
     tRNA            4540..4611
                     /locus_tag="IXT21_002543"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4572..4574,aa:Gln,seq:ttg)
     gene            5126..5833
                     /locus_tag="IXT21_002544"
     CDS             5126..5833
                     /locus_tag="IXT21_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4869910.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5893..6537)
                     /locus_tag="IXT21_002545"
     CDS             complement(5893..6537)
                     /locus_tag="IXT21_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ4869911.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6664..6951
                     /locus_tag="IXT21_002546"
     CDS             6664..6951
                     /locus_tag="IXT21_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869912.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7059..7379
                     /locus_tag="IXT21_002547"
     CDS             7059..7379
                     /locus_tag="IXT21_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4869913.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7460..8773
                     /locus_tag="IXT21_002548"
     CDS             7460..8773
                     /locus_tag="IXT21_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ4869914.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8770..9489
                     /locus_tag="IXT21_002549"
     CDS             8770..9489
                     /locus_tag="IXT21_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ4869915.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9617..10168
                     /gene="raiA"
                     /locus_tag="IXT21_002550"
     CDS             9617..10168
                     /gene="raiA"
                     /locus_tag="IXT21_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ4869916.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3424 a         1693 c         2190 g         2959 t
ORIGIN      
        1 tttttttgca tatttttagc atttatttcc taaaaaatgt aaggggattc ctatattttt
       61 aagtatactt gtttttcctt ttatacattt tatggtatta tcattttatt agttattata
      121 aaacaatcgt tagaaaggac gtcattatgc aagtatacgg aattgatgcg gtgcttcgaa
      181 tcgtgagtca tttggcgttt atctacttag ccttctggtc tttacgttcg ttacgcattg
      241 aaactttttt cagaccaatg cataatacgc aaattagaat ggcaatcgta cttttttcaa
      301 tttttttagg gtacactgca agtaattttt tcttagaagt tatcgcttta tgccgaaatc
      361 ttttcgttag cctgtaaatt ttaaaaaaag gtactatttt aaaagcaagg atgttataat
      421 agaaaagatt ttgagtaagt gtatgctcat tggaattttt tggagggaac aacatggaag
      481 agatcatcgt aagaggtggc aatcaattaa atggaacagt aagaattgaa ggagctaaaa
      541 atgctgtttt gcctatttta gctgctagtt tgttagcaga agaaggtatt acgacattag
      601 acaacgttcc gattctttct gatgtcttca ctatgaatca agtgattcgt cacttaaacg
      661 tagatgtaga ttttgatgaa caaaagaatc aagtaacaat cgatgcttca agacaattag
      721 aaatcgaagc gccttatgag tatgttagtc aaatgcgtgc ttcaatcgtt gtgatgggtc
      781 cgctacttgc cagaaatgga catgcaaaag tagctatgcc tggtggatgt gcgattggta
      841 aacgaccaat cgatttgcat ttaaaaggat ttcaagcttt aggagcaaaa attattcaaa
      901 aaaacggcta tatcgaagca attgcggatg aattgatcgg taacacgatt tatttagatt
      961 tccctagtgt aggtgctact caaaatatca tgatggctgc cgtaaaagca aaaggaacaa
     1021 ccatcattga aaacgtagct cgcgaaccag aaatcgttga tttagcaaac attttgaata
     1081 aaatgggcgc tcaagtctat ggagctggta ctgaaacaat gcgtattgaa ggcgtggatc
     1141 atttacatgc agtcaaccac tcaatcgtac aagatcgtat cgaagcaggt acctttatgg
     1201 ttgctgctgc aatgacacaa ggcaatgtgc tgattgcaga tgctatctct gaacataacc
     1261 gcccattgat ttctaaattg atcgaaatgg gtgcagaaat cattgaagaa gaaggcggag
     1321 tccgtgtcat cggaccaaaa catatcttac ctacagatgt aaaaacgatg cctcatcctg
     1381 gtttccctac agatatgcaa gctcaaatga cagctatcca attagttgca gaaggtacaa
     1441 gtgtagtgac tgaaacagtg tttgaaaatc gtttccaaca tttagaagag atgcgccgta
     1501 tgaacgctca tgtcaaaatc gacggaaatg tagcaatcat ggatggaaac catgaattgc
     1561 aaggtgcaga agtatatgca acagacttgc gggcagcagc agcattagtt ttagctggct
     1621 tgaaagcaaa tggaatcaca cgtgtgcgta acttgaatta tctagaccgt ggatattata
     1681 atttccatat caaactacag caattaggtg cagacgttga acgtgtagac atggatcaaa
     1741 cttctgctga aaaaacagca caaacgattg cttaaggaag tggctgaatg agtaaccaac
     1801 gatatatctt attaactttg ataaagattt tagtggtgat cttattgctt atccttcttt
     1861 ttgtggccgg aacgatgatc ggatatggtg tcatcggtgg cggcaatcca tttaaagtat
     1921 tccagccaag tttgtggatt catattcgag acttttttca ttgataaata agaatcacag
     1981 tggttctagt cgatatgaag tgagccatga agcagaacat gacaaaagaa aacagaaggg
     2041 aacgttgacg ttagtcgcga tccctttttt tagtaaaaga aaagaaggaa tctaattgat
     2101 gaagaagata ttgatttatt tagttagagg atatcagcgc tttatttcgc ctgcatttcc
     2161 acctagctgc cgttattacc caacctgttc caattatatg ataaaagcaa ttcaaaccca
     2221 tggtgcacta aaagggacgg taatggggac tgcacggatc ttacgctgtc atccttttgt
     2281 caaaggcggt atcgattatg taccattgac attccaatta acgcgaaata agaaagataa
     2341 aaaagaacca gattataaaa aggttgtgaa aaatacgagt agcactgagc gataaagaaa
     2401 caatttggaa aatagcttct tatattttgc aaaattgtga gttattgccg aagtgttgga
     2461 ttttgaacac cgtcaaaaaa aggttgtgaa aaatacgggt agcactgagc gataaagaaa
     2521 aagaggaaaa ttattgtcaa agtaacaact ccgcaactac ttatatgggg gacaaaaaat
     2581 gatcgaagaa ctaaacaaga aagatttacc atggtccttg ttattagatg cagatcctga
     2641 taaagaaaaa gttcaagtat atgtttccag agggagtggc tggatatgga aagaaaaaga
     2701 aaaaacaata ggtgtactga tctatgtggc aagggaaaaa gaatacgaaa tcgtcaatgt
     2761 agcagtagcc ccaacgtatc aaggaaaggg gattggcgga aaattacttg agacagcttt
     2821 tcagaagctt tcacaactcg cttccagtca aactcagatc atcatacgaa ccggcagtac
     2881 tagcagtgcg gcgttacatc tctatcaaaa aatgggctat gtggagattg gtcgagtcaa
     2941 agactatttt atccatcatt actctgagcc catttttgaa aatggagatc aattaaggga
     3001 tcaagtaatt ttagcaattc acttacctaa aaatagcttt aactaataaa ctttcaattc
     3061 gagaaaatgc tttcaaatgc taaatagata cattacaata gctttagcaa acaaaaagga
     3121 gaatattttt atgggaatat tagtttttga ttttggtgga tcggctgtca aatatggttg
     3181 ttgggatggc aaagagatta aaggtaaggg acaatttgca acgcctgaat cttgggaaga
     3241 aatgaaagcg caattatttc aagtttataa aaaaatactc gtttcttttg aagtcgaagg
     3301 aattggaatc agctctcctg gagtagtgaa taatgagaaa caggtgattg agggaatcag
     3361 tgcgattcct tatattcatg gatttaatat tttccgagat ttggaaactc tgtttcaact
     3421 cccagtaacg attgaaaatg acgccaactg tgccggaatg gctgaatttt atgaaggtgc
     3481 tgcccgtgat tatcaagatg ttgcttttgt tgtggcagga actgggattg gcggggcgtt
     3541 gttcacgaaa ggacaaatca ataaaggggc acatctttat ggtggagaat ttgggttgat
     3601 gttcttaaat ggcgataaga cattcagtaa acttggtaca gcggtacaga tggcttggcg
     3661 ttattgtgaa cgcaaaggtc tcgataaaaa ttcttatacc ggaaaagatg tctttgaatt
     3721 agcagaaact ggagatgcaa tagcaaaaga agaagttgag agtttttata cgtatcttac
     3781 aaagggatta ttcagtattc aattttcgtt tgatccagaa gtgatcgtat taggcggcgg
     3841 tgtttctgca aaagaagggc tcattgatga gatcaaaagc cgaatgaaga agctgacaga
     3901 acaattcgat ctgcatgatt tcgaaccgca gattttatta tgtgaataca gaaacgatgc
     3961 taatcttgta ggtgcagcag caaactatct cgctgtacgt caaggggaga attaagagct
     4021 ttagagaagg tgcagcttgc ataaaagaag tgaatgaaat gaataggaga tttttaccta
     4081 aggaatgttt tatttgctgc agtatgataa aatatctgag aggtgaaaat gatgtcaaaa
     4141 aaaaataaag atgttgaagt gcgtatagaa gaagtgaaaa aagatatcaa aggtaagaat
     4201 tatgaagtca ctcagttatt tatagggaaa aaaatgattg gtgagatctt agcctatggg
     4261 ccaaaagaat atgaaatttt ctttggtgaa gaagattttg gaaaagaaaa aagtcttgaa
     4321 aatgcgatag aaacagtgat tcgacactgg aatctgcacg aataaaaaaa tatcaaaaaa
     4381 actttcaata aagggttgca aaaatctgga aaattaggta ttataactaa gtcaggttga
     4441 tacaaacctt tcggaggagt agcgaagtgg ctaaacgcga cggactgtaa atccgttcct
     4501 tagggttcag tggttcgaat ccactctcct ccatttcatt ggggtatagc caagcggtaa
     4561 ggcaagggac tttgactccc tcatgcgttg gttcgaatcc agctacccca gtataccaca
     4621 atcgtaagct ctcgttagtt cgagagctct tttttttgct ttgctacctt gttccttgaa
     4681 gattctgctg atttaagata gagttaagtt aactacaaga attggaggga tccacatgaa
     4741 atggaaagaa aaagtagacg aagcagctga aaagttatat gatttggtca aatcagaaaa
     4801 atacaatatt gaagtgaata ttccgaaaaa aggcggaaaa gcggtccagg taaaatcaaa
     4861 acgcccaacg aatcatacaa aaaaatggat ggctaaaaac cgctaataat aacaaaagat
     4921 ccataagtcg gcattttcat cgattttagg gtcttttgtt attttctagg ttgaacatat
     4981 ctaaaaaatt gtttctgaca ataggagtag gaagaacgat gtgtgtctaa atcctctgtt
     5041 gatggataga aacctttatc tttttcagtt gtgaatcagg tatataccag ttataataga
     5101 agaaacgaaa ggacgggtgt acgttatgtc gaatcaatta cctgtatata tacagattca
     5161 tgaccaatta aaaaatgaaa tcgaacaagg tatatggaaa gtcggagacc gtcttccttc
     5221 agaaagagaa ttagcagtga aattcaatgt cagccgtatg acgttgagac aagcaattca
     5281 aaacttggca gatgaaggaa tcttagaaag aaagatcggt tcgggtacat atgttgcgcg
     5341 agaaaaagta caagaaaaaa tgtctggtgc aacaagtttt acagaaatta tggaatcgca
     5401 aaatcgagta ccgtctagtc ggacaatctc ttatttcttg acttcgccaa gctcaagtga
     5461 aatggaaaaa ctaaacttga gtaaagatga cacgatcttg cgaatggaac gtattcgttt
     5521 cgcagatgat gtccctattt gttttgaagt tgccagtgtg ccacaaaagc tgatcgatgg
     5581 ctacagcaaa gcagagatta ctgaatcgtt gtatcgcacg ctagaagaaa aaggcgggaa
     5641 aaagatcggt ccagcgaatc aaacgattac tgctgtgttg gcttctgaga aaattgctga
     5701 ctatttagag gtaaaacgag gagatgcagt tcttcgtttg cgccagattt cttattttga
     5761 agacggtacg ccgtttgagt atgtgcgtac gcaatacgta ggaaatcgtt ttgagtttta
     5821 tttggaaaaa taaaaaacag gtgatccctg attccttact tagaaataaa agagaatcag
     5881 aatcacctgt ttttatatat gatcagttac cagttgctcg tgataagaaa caggaccctc
     5941 ttcaaattgg acttgtccgt tcagcagctc aatgatggat tctttaaagt ggtcagcact
     6001 tttttcatct acagcacaga tgacgctaac ttgatcagta tattggattt cttttataaa
     6061 aaaatgttgg agttcaagat aattctgtaa ttttcctagt tgcggatacg tgatcatcac
     6121 agagatctcc tgttgcaagg taccttctac gattccaatg tcagacaatg cttgagagac
     6181 agcatgagtg tatgctcgta ttaatccgcc tgtgccgagt ttcgttcctc caaaatatcg
     6241 agtgactact gcagctacat tgatcagttg attttttttg aggacttcca acatcggtac
     6301 gccagccgtt ccacttggtt ctccatcatc gctgcttcgc tggatttcat ttttttctcc
     6361 aatgatgaag gcgctgcagt tatggttcgc tttccaatgt tcttttttta tttcttgaat
     6421 gaaacttttt gcttggtcct ctgattctat tcgtctaaga gaacagataa agcgtgattt
     6481 tttgatctca atctcacttt gtccattttc ttttattgta cgatagcgct cgatcatctt
     6541 gtcacctctt tttttcattt tctttattca gtataaataa agttctaggt tgtttcaaca
     6601 ctgtaacggt tgcgcaatcg ctgtagtatg cgataataaa gacaaaggta ggtggatctg
     6661 gggatgcagg aacgaatgaa aaagtacgat gcaattacgc attatttgaa gaacaatggc
     6721 ggctctcaag taacgttgac ctttacccaa tttgacgaac tgctgtttcc cagtaacgga
     6781 cttcccaaaa cagcaaggga aagtacagat tggtgggcaa atgattataa acaccctgaa
     6841 aaaggtgcct atggctggat aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa
     6901 gaatatgtcg tgttcaataa attagtgaaa tctagttggc tatttgatta aataaaccgc
     6961 gtttagaaaa cgttgacaaa tttctttctt tttatataat agagtaacag acgcgaagaa
     7021 aacgtttgcg tagtttgttt taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc
     7081 ttgtatgttc ggctggtatg agcacaagtt tactagtaac aaaaatgcaa aaagctgctg
     7141 aagcaaaagg aatcgactcg gatattttcg ctgtgtccgc ttctgacgca gataacaatc
     7201 ttgcaaataa agatgtagat gtactgttat taggacctca agttcgcttt atgaaagctg
     7261 attttgaaaa acgtctggaa ccaaaaggca ttcctttaga cgtcatcaat atggctgatt
     7321 atggtatgat gaatggcgaa aaagtgttag atcaagcaat cacgctcata ggtgaataaa
     7381 tagaacagta taactatcga acaagcgatc caaaaaatag atcgcttgtt ttttcgtata
     7441 tttttttagg gcgaataata tgaatcattt ttggggtaga aggcttctga cgagagatat
     7501 taaagcaata gactgtgagg aggtaaattt accaaattat gagaagatcg cagcagtgga
     7561 acgtttgggc aactggattc tttgtcatcg ctgtggaatg aggacgtccg tcattgatgg
     7621 acagcttgca gattacggtt atttttgtac acattgtcta tatttagggc gatgcgacac
     7681 tcaacaagat ttatatttgt ttgatcagcc tgaagcaaaa cctcgagaag tcgtcttttt
     7741 ttggacagga cagcttactg aaaaacaaac ggaaatcgca aagagaatac tttgccattc
     7801 agaaaaaaga catcatctta tttgggctgt aacaggtgca gggaaaacag agatgcttta
     7861 tcctatttta gtggaaactt taaaagctgg aggaagagta gcgatttgta caccgcgagt
     7921 cgatgtttgt aatgaattgt ttttacgcta tcgttatgtt tttccagaag aaaaaatcac
     7981 gttgctccat gggaatacag caacggctta tacctttact tcttttgtta tctgtacgat
     8041 tcatcagcta taccgttttt atcggtcctt tgatttaata gtgatcgatg agatcgatgc
     8101 gtttccttat aacggggatg caggattagc tcatgctgtg caaacagcga ttaaaccaga
     8161 cggacgattc atctacttaa gtgccacacc tgataaaaag ttactaaagg aaatcaagca
     8221 gacgtttgac atccacaaac ttgctttgcg ctttcatcgg cgtttacttc ctgaacccgt
     8281 cttggttttc atgaataatt ggcgacaaaa atgcaagaac aagaagaaga taaaatcttt
     8341 ggtgcggatc attcatcaat taattaaaga aaaccatatc cttttatttt gtccaagtat
     8401 cacgatgatg catcgtttga aagccacact tcaagaggct cttccgagat atgagatcac
     8461 agatgtttct tcaagagaca atcagcgggc ggaaaaagtc cagaaaatgc gagaagcaaa
     8521 atatgacata ctgttaacca cgatgatcct tgaacgtgga gtcacatttg aaagaatatc
     8581 cgttatcgtt ttaggagcgg atcatccggt ttttactaaa tcttctttgg tacaaatcgc
     8641 aggaagagcc gatcgtaaag ggccttatac gaacagtcaa gtatactttt tctatgaaga
     8701 aaaaactgca gctattcgaa aagcttgtca ggaaatcaaa gaaatgaatg aagaagggaa
     8761 gaagatgctg tgaggtgcag ttgctgcagt aaagatatcg tccaaaattt aacattcagc
     8821 gagattattg gtgtttcgaa atacagatta cctcagactt gttcagtttg tcgcgcacaa
     8881 tttcatttat tggaaacaag aggctgttct ggctgtcaaa aaccaatcca tttcaatcta
     8941 gagagtacga aaaagaaagt aaagtctatt cccgtttact gcagtgactg tcaagaatgg
     9001 aagaagaaat atccgaccta tgattttcag caccgtgcct tttttgtgta cgatgaaaat
     9061 atgaaggcct ggctcaatca atacaagttt atgggagata tccgactagc aggcaccttt
     9121 gcatataaat ggacgcagat aaaaaaagaa tacccgacat acatctattg tccaatacct
     9181 ttatctgaaa aaaggcgaac agaaagagga ttcaatcaag taagcgagat gttgaaagca
     9241 gcaagaattc cttttcagat gctactcaaa agagagaagc atctgctggc tcaagcaaaa
     9301 aacacgcgaa aggaaagatt ggagatgcct caaccttttg aacttgcagt caccttttct
     9361 aagatacaaa ataaaaaaat attgattatt gatgatgtct atacaacagg acagactctt
     9421 ttccacgcag cagattgttt gcaggtcgca cagccgaaaa tcatccgaac catttcttta
     9481 gcaagatgaa ttagatgtgg aaatttgata gcgatttaat ttgcaaataa cctagagttc
     9541 gatataatag aattaagaag agggaaagag tggtccttct ccttcttgat tgtggtagac
     9601 gaaaggggta atttttatgt ttagatataa tgtacgcggc gaaaatatcg aagtaactga
     9661 agcgattcgc gactacgttg agaaaaaagt aggcaaacta gaacgctatt tcagtgacgc
     9721 acccgatgca acggcgcatg tgaacttaaa agtttataca gaaaaaacag caaaagtgga
     9781 agtaacgatc ccattacctt atcttgtatt acgtgccgaa gaaacttctc cagatttata
     9841 cgcaagtatc gatttggttg ttgacaaatt ggaacgccaa atccgcaaat tcaaaacaaa
     9901 aattaatcgt aaatcccgtg aaacgggcat gaatactgca gatgctactg ctttattcaa
     9961 tgaagaggac gaaaacgaaa atgattctga attagatatc gtacgtacga aacgcctttc
    10021 attgaaacca atggatagtg aagaagcagt cttacaaatg aacatgctgg gacataactt
    10081 ctttattttt gaagatgcag aaacaaatgg tacaagtatc gtttaccgcc gtaaagatgg
    10141 aaaatacgga ctgattgaaa cagactgatc agccttactt aaataacaac gaaaacttgc
    10201 agcagctact gacagctgcc gcaagttttt ttgagtttcc tcctctttta gatgaaatat
    10261 gaataa
//
LOCUS       DACGLH010000097         9889 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.102, whole genome shotgun sequence.
ACCESSION   DACGLH010000097 DACGLH010000000
VERSION     DACGLH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.102"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(37..282)
                     /locus_tag="IXT21_002551"
     CDS             complement(37..282)
                     /locus_tag="IXT21_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869917.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(397..975)
                     /locus_tag="IXT21_002552"
     CDS             complement(397..975)
                     /locus_tag="IXT21_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4869918.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1052..1582)
                     /locus_tag="IXT21_002553"
     CDS             complement(1052..1582)
                     /locus_tag="IXT21_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ4869919.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1586..2446)
                     /gene="rlmB"
                     /locus_tag="IXT21_002554"
     CDS             complement(1586..2446)
                     /gene="rlmB"
                     /locus_tag="IXT21_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ4869920.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2439..2834)
                     /locus_tag="IXT21_002555"
     CDS             complement(2439..2834)
                     /locus_tag="IXT21_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ4869921.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2831..4243)
                     /locus_tag="IXT21_002556"
     CDS             complement(2831..4243)
                     /locus_tag="IXT21_002556"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ4869922.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4240..4776)
                     /gene="cysE"
                     /locus_tag="IXT21_002557"
     CDS             complement(4240..4776)
                     /gene="cysE"
                     /locus_tag="IXT21_002557"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ4869923.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5098..6558)
                     /locus_tag="IXT21_002558"
     CDS             complement(5098..6558)
                     /locus_tag="IXT21_002558"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ4869924.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6728..7885)
                     /locus_tag="IXT21_002559"
     CDS             complement(6728..7885)
                     /locus_tag="IXT21_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ4869925.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7919..9289)
                     /gene="radA"
                     /locus_tag="IXT21_002560"
     CDS             complement(7919..9289)
                     /gene="radA"
                     /locus_tag="IXT21_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ4869926.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9352..9879)
                     /locus_tag="IXT21_002561"
     CDS             complement(9352..9879)
                     /locus_tag="IXT21_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ4869927.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2515 a         2219 c         1786 g         3369 t
ORIGIN      
        1 caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc
       61 gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt
      121 ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacgccgcg
      181 gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg
      241 accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat
      301 caactatacc acatattttt aaaattttca agtttaccat aaaaaaaaaa gaaatgcaat
      361 aaaaataaat aagaagctca ttaagatttt tctaaattat tcctcattat aaaatttcaa
      421 gtgttttttt attttgagtt ttactctccc gtaagcgctc attacttgct tgtgatccat
      481 tgctaaacat tcagccatgt ctacaaaatc aacgccttgc atataataat gaaaaatttt
      541 agcttctatt tttgaaaact ggatttcgca attttcgatt gcttctttta ataaaagatg
      601 ctgaatcgct aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc
      661 tattttttgg tcgattgaga cttcatcttt ttgatcacga cgcttcagcg cgttctgctt
      721 cctcaacaga gaatagattt gattgcttat aatgctttta taataacttc caaagctaac
      781 acctttgctt ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg cttcttgttc
      841 ccaatcctca gaaacataat ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg
      901 aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa
      961 taactcctca gacataaaat aattcctcca taaattcgtt tatggaggaa tcttatcatc
     1021 aatgattcat cgaactgatt agaacgatat gttatttatt ttttctcatt aaatcgtcca
     1081 atttttggga caattttttt aactgctctt cattccatgg agaattacgt cggaaatcaa
     1141 taaaatgcat atctttattt tcttgggcta ttgttttttc cgtttttttt atcgtcttat
     1201 ataattcata agcagaagtt cgtaatgctc cttgggaaaa gatcatccac tgttctgcta
     1261 aatcgcttgt cgccactgtc acttgtgtca aacggtcatt cagctgacca gcgattctct
     1321 cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac tggtatttct
     1381 tataattttg ttggatgccc ggaactagtt gggcatcgaa gacaacgatg acttccattc
     1441 cttcatattt cgcataattt gataaacgat gtaaaagagc ttctcttgca tccgccattt
     1501 tgtcctgctt tttaagctgg acaagttccg gccacgaacc aatcatgttg tagccgtcta
     1561 caatcaacag ctgttttttc atggtctact ccccctgacg ttgggaaaaa gcctgataca
     1621 taagcaaacc agcagcaaca ctggcattta agctctggac gtgcccggtc atcgggatcg
     1681 tcagcaattc atccatttct tttttcaatc ctggactcat tcctttgcct tcattaccaa
     1741 tgactaaagc aattgatcct ttggcattcc atttttggaa gtttgtgccg gtcatatctg
     1801 tgccaaatac ccaaaatcct gtttctttga gctgttgcac agcttgtacc aaattcgtca
     1861 cgcgtgcaac agggacatgt tcgactgccc cagtggaagt ctttgttacg ataggtgtga
     1921 ttccaactgc tcggtgtttt ggtatgatca caccatcgac gccacttgcg tctgctgtcc
     1981 gtaaaatcga cccaaaattg tgtggatctt cgatgctatc caaaatcaaa aagaaggggt
     2041 cttctttttt ctcttttgtt cgttcaatca gttggtcaag ggataaatat tcataaggtg
     2101 taatcgcaag aacgactcct tgatgcacgc cgtgatcgct caaagtttct aatttttgtt
     2161 taggaaccca tttgactggc acagcttgtt cacgtgcgat ttctttcagt tcttctactt
     2221 tttctcctcg tgaatcttct tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt
     2281 ctactgcggc atggtgccca aagacgaaat tatcttctaa tgcttcttca ttttgatcag
     2341 aaactgactg atttcgtcca gtttgctttt tatttgaata tctattttgt tttttgtctt
     2401 caaatccacg tttcgattga tgtttgtttg ttctttcttt acccattttt ttcaccaact
     2461 ttctgaatac accagcggat caattcttcc atccgctcgg tttgttttgt caaatgcaga
     2521 tagcccatga gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc agcatttttt
     2581 gcgctcgtat ggcttttagc atttcttcct ctgcgataat aaagttcttc ttcttctgtc
     2641 aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa
     2701 ttcgttgccg cacgatgcag cttattgggt tttgtttctc cttgatcaat caggtggtca
     2761 cgaacataga tttcgtaaat cgcatctcca acataagcca aggctaaacc gttcaattgt
     2821 gtgtaatctc tcatgcttct cttctccatc gggttccttg cggtgtatct tctaataaga
     2881 tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc
     2941 ttgcttgatt tcgttcttcg atcaattgtt caatctcttc atctaataac tcttctgctg
     3001 taaataaaat accgaagatc gaaagccatt gcgagaataa agcttctcct tgtgccaaga
     3061 cagcggcaga aactttcggc tggtctagat aagtgttcag ccacttagca agctcataga
     3121 ccactgtgat cccatttgct gcattaaaat catcgtccat ttcttcgatg aatcgctgtt
     3181 ctaaattttc taacaaagcg attttatccg catcatctgc caaattctct gaggcagttt
     3241 cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt
     3301 ctttcatcgt cgtttcactg tagcggatcg gacgacggta ttgtgtggtc gccatgaaga
     3361 aacgcaagac ttgtggatca acgtttttga tcatttcatg tacagtgatg aaattcccta
     3421 gagatttact cattttttca tcgtcttcgc caatcgttac atagccattg tgcatccagt
     3481 aattagcgaa ttttttccct gttttcgctt cactttgtgc gatttcattt tcatgatgag
     3541 gaaattctaa atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg
     3601 ccataacgga acattcaata tgccaaccgg gacgtccttt gccccatggt gaatcccaag
     3661 aaatctcgcc aggttttgct tgtttccata aagcgaaatc taatgggtct tctttttgct
     3721 gctgttcttc ccctgtacgc tgacttgctc cgacttccag ttcatcgatg gattggtcac
     3781 ttaatttccc ataattttca aatttacggg tacggtaata cacatcacct gaagattcat
     3841 aagcatagcc tttttcaacc aacacttgaa taaatgctaa aatatctggc atatgatcca
     3901 tgactcttgg atgcagtgta gcaggctcga cattcaacgc tttcgtatct tcttcaaatg
     3961 ctcggatgaa acgatcagca acttctggtg ctgtaatacc taattcgttt gctgtacgga
     4021 tgattttatc gtctacatcc gtgaaattcg atacgaaatc gactttatag cctcgatatt
     4081 caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg agcatttccg atatggatat
     4141 aattatacac agtcggtccg cagacataca taccgacatg tcccttttgt atagggatga
     4201 attcttcttt ttttctggtc aatgtattgt agatttttat cattctgatc tcctcacttt
     4261 ttctcctttg attcgaacga cttttgctgg tactcctacc gctgtagcgt cgtctggaac
     4321 atctttcaac acaacggcac ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc
     4381 taatatctgc gcattcgcag agagcatggc tccttgttta acagttgggt gtcttttacc
     4441 cgtttctttt cctgtgccgc ctaatgttac gccatggaat aaaatcacat cgtcctcgat
     4501 ttctgcggtt tcgccaatga cgattcccat tccatggtcg atgaacacgc cagttccaat
     4561 cgttgctcct ggatggatct caacaccagt taggaaacgc cagaattgag cattgatctt
     4621 cgccaataaa aagagccggt gctgatataa aaaatgagag aaacgatgcc aaaacaaggc
     4681 atgaaaacca gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct
     4741 ctttgctgca tcaatcgctc gggtaaacca actcatgact ttccactcct ttcgtaagaa
     4801 ttttatgtag gaaatcatct tcttataaag aaaagaagcg ccttttaaat aagtttcctt
     4861 atcaaaagac gctttccgcg tggttccact tttcttcata atcaatgatt atctctgaca
     4921 gctaacgcct gttatacgtt cttggctact ttgtttccta taaaacagtt tttcctgttt
     4981 tctgtgtttc gccaagacca ctcccagagg catttcggca accattcatt gataccttgc
     5041 accacccggt atctctcttg taatgatcag ctgcttactt ttttctgttc atcgtgttta
     5101 gttcagtact tgatttaaat gtgctaatga tttttcacga ccaagcaatt cgatcgtttc
     5161 aggcaattct ggaccatgca tttgtcctga tacagctaca cggattggca tgaaaagatt
     5221 ttttcctttt acgccagttt ctttttgaac agctttgatg gcagctttga cagttggagc
     5281 atctactact tccatttgtt ctagctgtgc tttgaatgcg tggagaacag taggaactgt
     5341 ttcaccagca agtacttctt ttgctgcttc attcaataca ggatgttcat tgaaaaatag
     5401 cgtagacaga tcaacgattt ctgcagcata gctcatttga ggttgataca ggctaacgat
     5461 cttcttcacc cattcgattg tcttttcatc tgggttttct gctacacgtc catcagcgat
     5521 tagataaggc agacacatgt cagtaaatgc atctagatcc attgctttga tgtaatggtt
     5581 gtttacccat tgcagttttt tcgcatcgaa agctgcgggt gatttactta ggcgttttgg
     5641 atcgaacatt ttgatcaatt cttggtgttc gaagatttcg tcttcgccca caggtgacca
     5701 gcccaataat gcaataaagt tgaacattgc atctggtaga tagcctaatt cgcgatattg
     5761 ttcgataaat tgcaagattg attcgtcacg tttgcttagt tttttacctg tttcactgtt
     5821 gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt catagatcat
     5881 cagttgtttt ggtgtattgg caatatggtc atctccacgt aaaacgtgag tgattttcat
     5941 caaatgatca tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc gtttttgtat
     6001 gacaaagtcg ccaccgatat tatcggattc gaacgaaata gcgcctttca ccatatctgt
     6061 aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca acagattcta gaccttgtgc
     6121 ttctttttct gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat
     6181 ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg tgcaatagca
     6241 tttgtatgct cgattgctag caagcagctg atcaatcagc ggttgataga tttctttacg
     6301 ttcagattga cggtaagggc cgtattctcc aggattttca ggagattcat cccaattcat
     6361 tcccaaccaa gcaaggtttt ccaactggct tttttcgcca tcttcgatat tacgtttttg
     6421 gtcagtatcc tcaatacgaa taataaagtc tccatcattg tggcgggcaa acaaataatt
     6481 gaataatgca gttcttgcat ttccaatatg caggtgccca gttggacttg gtgcatagcg
     6541 tacgcgtact ttcgtcattt tcttcctctt ctttcaaact agactttgac taacagcaaa
     6601 aattccgtca gttacggctt ctcaaatatc atttatttaa agccactcag cacaaatttt
     6661 accttttcta aggtaaatag taaagccaac tggttgaatt tccttaccag ttggctgaat
     6721 ttctttttta ttttgattgt ttagtctttt tctctgtctc ttttttattg tcgattcctc
     6781 gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc agctgtttga agggcacttg
     6841 ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc atcttctacg acaaccatcg
     6901 tgccgtcatc aaggtaggct actccttgtt ggcgttctgt cccggctttt actactagaa
     6961 cattcatcgt ttcaccaggg atgacgactg gttttacggc atttgccaac gcattgatat
     7021 taagaacagg tacattttgg aattcagaga ctttattcaa gttatagtca ttggttacaa
     7081 tgacaccatc taaaagctta gccaatttga tcaatttgct gtctacttca ctgatatctt
     7141 caaagtctcc atcgtacatt tcgacagata taccctcttc tttttgcaag gcatttagga
     7201 tatctaatcc gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt
     7261 gtagttcgta aagaacaaaa ttagggatca tcaaggttcc ttctaagaat ccagtctttg
     7321 taatatcata aatccgaccg tcaatgatca cgcttgtatc caagatttta tatttatgga
     7381 aattttctcc tgattttcgt tctaacagtt gactctcaac ttgactagct gtttcttgct
     7441 cgattttttt gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt gtcgttccca
     7501 ttctcaatcc cagatagcct aagatgatca tcagtaaaat cggcaataca ctattgacga
     7561 atggaatttc catattgtaa agaggggtcg agatcacaac acctaaagtc aatccgatga
     7621 tcgcaccgat cgcaccgaag agaagataag tcaagctgac ctcacttagt ttttgttcta
     7681 cttgtttgat aatagctgag atatgtttgg ccaacagcaa agataataaa aagaaaataa
     7741 gtgcgcctaa caggctgttg gtaaatgtat tattcagcca tccgtttgac tgttgaccga
     7801 ttgcctgcca agctgagggt aaaaatgaaa tacccaagct tgcgccgaca atgatcatca
     7861 gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca tttattagtt
     7921 agcggaacac acgcttcaac gtttcagcca aagttgctac tccaactatc tcgattcctt
     7981 ttggcgcttc ccagttacct aagttgtttt taggtaggta tacttttgtg aagccgagtt
     8041 tttggacttc tttgactcgt tgttcaatat gactgacacg tctgatttct ccagtcagtc
     8101 caatttcacc gatgaaacat tccgttggct gtgttccttt ttctttatag cttgaagcaa
     8161 tactgacagc gatagctaaa tcaatcgctg gttcatttaa ttttacccca cctgcagctt
     8221 ttaagtaagc atcttgattc tgaagaagca acccagctct tttttccagc actgccatga
     8281 tcagagatac ccgattgaaa tcaagacctg ttgttgttcg tttggcattt ccgaacatag
     8341 taggtgtgac taatgcttgg atctctacca aaattggacg tgtcccttcc attgccacaa
     8401 cgatagcaga gccagttgca ccgtctaggc gttcttctaa gaaaatctgt gaaggattag
     8461 cgacttcttc caatccatgc tcatgcattt caaaaatccc aatctcattc gttgagccaa
     8521 atcggttttt tactgctcgc aaaatccgaa aactatgatg ttgttcacct tcaaaatata
     8581 acaccgtatc caccatatgt tctaacattc ttgggccggc gatcgagcct tctttggtaa
     8641 catgaccgac gataaaaatg gctatcccat ttgttttagc gattttgagt agttctgcag
     8701 tggtttctct tacttggctg acacttcctg ccacacttgt gatgtctggc tgcgtcatcg
     8761 tttggatgga atcaatgata acataatcag gggagatatg ttcgatggca cgactgattt
     8821 cgttcatatc tgtttcagcg tatagataaa attctgtgtt gatacttccg aggcgttccg
     8881 cgcgtaattt tatctgctct gcgctttctt cgccggatac atataagact tttccgccta
     8941 tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga tttcccgata cctggatcgc
     9001 caccaatcaa gaccatagac cctggcacca cacctccgcc taacacacgg ttcagttctt
     9061 ctaattttgt tttgatccga ggttcttttt tagggacgac gtctgcgatt tttgttggtt
     9121 ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct tgttcgattt
     9181 cttcgaccat cgtattccat tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca
     9241 catacccgca attttggcaa acaaattgta cttttgattt ttttgccatt gtaccgcctc
     9301 cttttatcct aatcatgaga cattaaatca agcttaagta tctttaacgt tttatttact
     9361 ggaagaacca aatccgcctt gacgttgagc agtcgtttga tcttgatctg cttttaaaaa
     9421 cggcaaaaag atcccttggc cgatacgctc gccttttttg atcactacat ccttgaaacc
     9481 aaagttggtg aattggaaca tgatatggcc ttcatttgcc tcattattgt aataatcact
     9541 gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg
     9601 gttagcaagc tgcaaaaatt catcttctcc catgtaagct ttgattccag taggcacgag
     9661 ggttggtttc aattgttttt cattttctga tacatctttt ttcaatccat ctgcgactgt
     9721 ttccaacccc tttttccagc ctgctttcca gatacttgga atcacgacct cttctgctgc
     9781 ttcaaaatca tagccagcag catgatgtgt agcgcgctgc ggtagattga tattttgatc
     9841 tttatattta gaaataactt caaatcctct ttttctcacc ggttttcct
//
LOCUS       DACGLH010000098         9692 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.103, whole genome shotgun sequence.
ACCESSION   DACGLH010000098 DACGLH010000000
VERSION     DACGLH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.103"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..505)
                     /locus_tag="IXT21_002562"
     CDS             complement(<1..505)
                     /locus_tag="IXT21_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869928.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTDESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQ"
     gene            complement(729..1613)
                     /locus_tag="IXT21_002563"
     CDS             complement(729..1613)
                     /locus_tag="IXT21_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ4869929.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1708..2145)
                     /locus_tag="IXT21_002564"
     CDS             complement(1708..2145)
                     /locus_tag="IXT21_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869930.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(2362..3273)
                     /locus_tag="IXT21_002565"
     CDS             complement(2362..3273)
                     /locus_tag="IXT21_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4869931.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(3730..4158)
                     /locus_tag="IXT21_002566"
     CDS             complement(3730..4158)
                     /locus_tag="IXT21_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869932.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(4186..4824)
                     /locus_tag="IXT21_002567"
     CDS             complement(4186..4824)
                     /locus_tag="IXT21_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869933.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(4828..6864)
                     /locus_tag="IXT21_002568"
     CDS             complement(4828..6864)
                     /locus_tag="IXT21_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ4869934.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6990..7655
                     /locus_tag="IXT21_002569"
     CDS             6990..7655
                     /locus_tag="IXT21_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4869935.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            9096..9692
                     /locus_tag="IXT21_002570"
                     /pseudo
     CDS             9096..9692
                     /locus_tag="IXT21_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3098 a         1829 c         1534 g         3231 t
ORIGIN      
        1 tctggaactc gatcgactga ggtatctcat tgatataaat atggtgtgtg ccgaaattgt
       61 gagcgaatcc accaaaatca ataacgatat ctcgtttgat ctctaattcc ctgtctaaag
      121 tcaaaggctg aatcagtcgg atcttagtta ctgattcgtc tgcgagaagc tgcgccagtt
      181 cttctgttgt ggcagcttcg ctgtacccat ccgctgaaac agctcttgcc agtgccgctt
      241 cactgctatc agacgtttgt tcagctactt ctgaggtctc acttgtctta ttttcttctt
      301 gccttttatt agtagaagcc tcactttgag attctgtagt ttcagaagta atcgattctt
      361 ctgaaaaagt agtcttgtca aaactttgat tctctgattg cgttgattcg tctgttttct
      421 ctacatcggc aaaaacggat gtcaccatca agagagggaa gacaaacaaa atcgacgcaa
      481 tcagcttttt tacacattga ttcattctcc accatcctct ctgacgtttt gacaatttta
      541 ttttatcgtt ttaatctatt tccaattgta ctttcatatt taaaatatgt atttttatgg
      601 aaaaattttt ctattaggtg taaatttctt ttttgtacaa aaaaaaggct gtgacaaaag
      661 tcttgtcaca gtcccttatc ccgaacaagc ggtgttctcc cgccaactcc tcggtcacaa
      721 cctccatttt aacaataaac ctgtaaatgt gagggcaata ctttgattgt cacaggtaat
      781 ttatcccctt catctccatc aacattcgtc gtcaattctt cctcatcagc taatgacaca
      841 actaattgct cacaagtaag gtaggctaga tttttcgtag attcatctac tccttttaat
      901 aggtctggca atgttttaag cgtatcgatc aaagaagaat cttttaaata aaccaaatgg
      961 agcttaccat catccacttt tgcatttgga agtaatgttt caaaaccgcc gactgagtta
     1021 gttagtccta tcagcaaggt actgctctca atttcttcct tctttccatc aacttctacg
     1081 tgaaacgagt aggattgtgt ggaagctaac tgtttgattc ctgacatgaa ataagctagt
     1141 tttccaaatt tcgtcttctt ctccgcatct acatcattga ttgattcagg gatagagcca
     1201 attgcgacga cattcataaa atagtcgtca tttatttttc caatatccaa tggcttgact
     1261 gattcaatcg aaaaatgatt gatcgcttcc tcaggttcta gcggtatccc taatgctcga
     1321 gccaagtcat tcaccgtacc caatgggaaa aatccgaaat ttggccgatg ttcttgttca
     1381 gcgattccac tgattccttc gttgactgtt ccatctccgc ccatgacaaa cacactatga
     1441 tacccttccg tagccgcttc gcgagtaaag ttttttgcat cccctgcttt ttttgtatgc
     1501 aagacgacca cttcatcaaa caccgattcc aattttgcta tcgctaattg ctcaaattcc
     1561 tttgcttgtt caccgcctga actggggttt accactaata aaactttttt catcactttc
     1621 tttcccttct gattgatttc tttaatataa cgatagcata aagaaaaaaa ttaggctgtg
     1681 atgaagtact gttcatgaaa cattcattta ttttttgaca agataaaaca tccatactat
     1741 gaaactgagg aaaattaaaa caatgatata ggaaataaaa gaaaatgata ggcattgtat
     1801 acctaataaa gtaaaaatct taggtggcag ataaaaacac aaaaatagca agagaatgta
     1861 atagcgtttc ttccactttt tgttatcaga ccttatagcg atcagaggaa taccaaatgc
     1921 ataactgaaa aactcttgat gtactgttaa tatgtcacgg ccttgaataa taaatgcgac
     1981 aatccccgct ataattagtg tgcctaacac caaaacggaa aattgatgaa tatattttat
     2041 atacaaatga tccattgtac ttcctgcctt tttatttgtg agaaaaacaa atccgtatac
     2101 gtgaataact tctagaaata aaagaaattt gcaaaaccac tccatttatc tacttctacg
     2161 tgcatttttt gatattgttt tctttattta agattataca gaaaaatatc tagggtcgct
     2221 ggattctaag taaaaaaaat ttttaaaata aaaagaacta aatcaagtag taattaaact
     2281 actgttgatt tagttctaat aaaaagattt agatgaacag ttttattgat ttaaatagtt
     2341 catatttttt atttataccg cttatttact acttgctgta ctagaatctg aagtgaatgt
     2401 actcaaaaca ttttcaaagt ctttgtcttt gatttttaca ttagcgtctt tcaatacttc
     2461 gccaattact ttattctgga aggtactgtc acttaatttt gtgtctgttg caatttcttt
     2521 caattctttt ttgtatttat ccatgtcgtt gcctttgttt tggtttttca ccattttaac
     2581 cacataatat tccgtagtat aagttgaagc attggtagaa gtgatcacat cagaaacttg
     2641 accatctttc aacttaaagg ctgcttcttt gacttctgcc ggaaccgttg tagtagtgga
     2701 atcaaatttc acttttccgc catcttcttt tgtcgtagta tcagttgact tgtctttggc
     2761 taatttagag aaatcgtctc ccttatcagc agctttcttc acatcttttg catcatcttc
     2821 acttgctact tggataattt gagcttccac ttctggatgg aaagattccc aagccgtttt
     2881 taaatcttca tcggtcaatt tgatatgttt ctttagacct tcttgaaagg ctagactttg
     2941 tttgatactg tctttgaatg tttgttctgt gtaacctgca gatttcaatt gtgaatcaaa
     3001 tgaatcaccc aattgtttct ttgtttgatc atattgagaa tcgatttctt tggttgatac
     3061 gtcatctcca tatttatctt caaatacttg ataaacgatc aactgttgca cagcggaatc
     3121 tgctccttgc atttttttca agctatccat tacttcttgt tcgctgattg taccgccttt
     3181 ataactaacg gctgtttttt caccggcatt cgtacagcct gacaataagc cagtgcctag
     3241 tacgatggca agtgctaaaa atcttttttt catttatcaa ttctcctact tttcgttttt
     3301 tgcgaattgc taaaaacaaa ggtgttcaag atgaaaatca tcaagaacac ctttactaat
     3361 ctgctcgata cgtcctgaat agatataagg ctacacaatc cagaaagttc gctcagcaga
     3421 ataaatgtca agtagcacat cacaccttaa tatactactt cttttgttac cgtttttcaa
     3481 gtctatcttg cgttccgtat ttaacgtaac taattcttag aacaaatgtt atcgattttg
     3541 tttttcttct tccgctgctg ggatattcaa atcagacact tttatgttcg attttccaga
     3601 catttttttg ttgtttttat tatgatatat ttttatacaa ctaaaaatga atatattaaa
     3661 actaccaaaa ataactttta acgctgtttc attatatgtt ttttctattt tatataaact
     3721 tttggatact taataccaac cggttggatt gtaccaacat gagaatgtgt cagttctatt
     3781 tcctgtcgca cttgcatagg cccatactcc ttttgttgca tttgctgatc gaatagagtt
     3841 tccaactttc gcttttgcac cgtgacgatt atagacacga ctatagtggt tagaatgacc
     3901 tgtttttctc gtacaagttg tatcactata ataaacccat cttccagagc tatgattaat
     3961 tgctgttcta gaaatttccc ggtctactgg taaatttgac ttaataatat tacctttagc
     4021 atcaaaatga atctctcctg gctttaaaga tttgtaatag atttcaccgt gaactgaatt
     4081 tgcttccact tcagatgaca tagcgtttcc tccaacaaaa cctatcccta gtgcacaaat
     4141 aataactgct tttttcataa aacgacatcc tttccttttt gatttttaaa gcatgatctt
     4201 cttgtttcct attttggtca gatatggatc atgcgtgact acgataacta acgtaccttg
     4261 ggaagcttgt tgcgataaga tttcaactac tttatcctta tttcttttat ccaatgaccc
     4321 agtcggttca tcggctaaaa tgattttgct attcttcaat aagactctcg cacaggcgat
     4381 tctttgcttc tcaccaccag ataatgtgta gataggcgta cttagcttct tgtttatatt
     4441 tacttttttc aatgcctcat actttgctgt tcgcttatct gaaacattta aattcgaaaa
     4501 cttcaatcca atatctaaat taaaatcgat tgtttcatat tcaatcaacc caaaatcttg
     4561 aaaaagaaaa gatatggtat ttctttttat caagcgcttc tctctcccag ttaattgttt
     4621 cgtctcttga tcgaacaaat agaattgtcc tgaactgaac tgatcattca atcctaatat
     4681 attcaataac gtacttttac ctgctccact ttctccaaca acagtcacaa gatccccagc
     4741 atccgcagtg aaacgatcca aatgaagaat cgttttgttt cgaatgattt ttttcgcacc
     4801 ttttatcact atcaacggct ccaattgtta acctccattc atcagcaata actgactttt
     4861 cttttctgta gacaaaataa aaacaactga aagaataaaa tcaatgaagc taaagaaaaa
     4921 aatattaaaa agatatttag gctctctttc tataagaaaa aggaagatta cacttgtcgt
     4981 tccccatttg atgaggttca tcatgaagaa atctttatac gtttcaaaga gcgaataacc
     5041 acttgaacga aagatagcga ttttccgaca attgtagaca aaatatatgc ataacatgac
     5101 atagctcaac aagaagaaag aaaagaaagt gagtaaaaat atctgtatca agtaatgaaa
     5161 tgatctgctt aacactttga cagctatctc ttcagtctga gtatacggaa atgataactg
     5221 caagttatca cgtaactgac tttcttcaat cagctcaaat aacctttctt tcgacaaaga
     5281 gcctatcttg atctttaacg gtggatagat ttctccgctg agaatatttc tttccaaata
     5341 catactgttt ttcaatgtca gtatttcaac aaccggataa tgttcgatcc aggggataga
     5401 aggaataaat gtataaatgg gttgattgtt ttttataaat agaaatttcg gttctttttc
     5461 actgatttct tgataatatt ctgttatttg cgccaggtca ttttttaaat ctgtatgtct
     5521 atatcgttca gggatcaata taacagggtg cgtttctttt tcggttattt ggatcaacct
     5581 tccagagaca tcaatcaaag gaaatttctt aagataattc agatttattt ttatccctct
     5641 gccaaactgt tcattttctt cattcaagta atctgataca ttgactaata tacttccgtt
     5701 tttattcaaa gtttcatata attcctgatc tgctgctgat gcaaaacgat tggaataaat
     5761 aaaatccacg tggtttttcc cgatggttac tggataaaat acatgatagt cctcaccagc
     5821 gatttctttt ggtggttcat gggaaacagc taactgtgaa gaagaaaaaa cgatagttgc
     5881 taattcgaca ttggcaacgg tcaaaagaag agaaaacaac aatccaaatg taaaaagaat
     5941 gtattggata tagttgaacg cttctcttaa cgaagcgatt ttttcaacta ataaaagaga
     6001 aatcaaagag ataccatata ttccgctgaa caagaaaacg tacatctgga acaaataaag
     6061 aaattgctcc ttataataca taaaaataaa accaccaatg attcctgcaa ttatgaatgt
     6121 aataatgatt ttctttttcc ccaataaaca ccacaatact tgatgtaatg aaaagtcatt
     6181 attcaacaat aaactgattt ctttattcat caataagaaa aaggatgata acaaaaggaa
     6241 taaaagaatc aatccaatag ttaaatacac tcgataatta tatacgtctg tatttgaaat
     6301 aaccgaatac gaatagctat tttcaaagtc ttcaaaaata ttagtggcat aatcagtgtg
     6361 aaatgcttga ttgaatcgat cagtgaatag tgaaatgacc cgttcatagg cttctttttc
     6421 tctcgtcatc aagaaaaatt caccttcaaa atcttttatt ttgatcgatt cttcaatgga
     6481 cgttacgcga aaggaggtat tagaataggg taccttctcg tgagaagatt tgacatcccc
     6541 ttgataatac aatgtcattt caagctttgg ctgtatcttg aagttccaag atgtatctac
     6601 ttcattggat tcatttactg tttttttcat ataagaaagc tcagtttctg atgagacatt
     6661 ttcaaatacc atgattagct gctcatattc ttcttgccca ataaaatgtt gtggcaatcg
     6721 aatcggagta taagtatctg tttgatggaa aaattcattt gtccgataga gtacctgttt
     6781 atttatacta accaaggcaa aaccactcat gataccggtc actatgattg tgagtaaaac
     6841 tgtgaacttt tttttgatta tcaccactct cccttactta aatgattctt tgataaaatc
     6901 ataccgtttt tcatcaaaaa aacgtatcta tcaagtgtat aggtcatgta gaataaatct
     6961 atatactgta gaatcgaaag gagaagaaga tgaacaccgg agaaaaaata cgaacttacc
     7021 gcagaaattg taagcttacg caaaaagaat taggcgaaaa aattggtgtt tccgataaaa
     7081 cgatttctag ttgggaaaat tctcgtacaa tgccagacct tgaaatgctg tcacttttac
     7141 atcatacttt aggtctgccg attgattttc cttctattga atcagctacc gaatcaatag
     7201 aaccaacatt gccagatgaa cctcttgtta acaagaaatc ttattcatca aaacaaccct
     7261 tctcactcaa aaagacacta tctttctttc tgctcgtctt ttgtttggtc tatctttttc
     7321 ttatcaatta tcaatacaaa aatgaaacga tcgatcgatt caatcctttt ttgaaaccac
     7381 aaatcggcta taccacactc cctgtaggat acgtggacaa ttccatcgaa cgttgggtat
     7441 gtaatgatat ctttggccat ggtacatggc tgtcttttca cgctggagag gtagaaaaag
     7501 agaaaaacca tgctgtaatc aaacacaaag gaaaacacgt catgaacgtt cgcctgctca
     7561 cttgggaagc cattccctca gattaccaag aaatcctcag ccaagactat gacgacgcct
     7621 taaaaagaag ccctcaggag ccagataggc attaagtagg agatgaacgt agagatagaa
     7681 aaaatcaatg cacatgatct tatccaaaaa acaggggtac aaattaattg aaaaagcctt
     7741 gagatgaaag ttcctaaata aaaaagttca gaatgatagg tatttctggc ttatagtata
     7801 atatttacgc cacagcctcc caatgattct ttttggaatc ataatcaagg atggacattc
     7861 acaaaaatat tttgctcatt cgatccgaat agattaacga ccaatctagt aagtaatata
     7921 gattggtacg tagtcaagct gcacataatt tagatcaatt ttagttcttt tttatatttc
     7981 tatgatctgc tcatttactt ttatatgatg gcttacagta acataaataa atatgataat
     8041 cgctggtagt ttatccaaga gtactcaata aaatacaaaa tggattgttc aagcgcctat
     8101 cataatgata aaatgaaaca aataaacgat acaaggagga agagactttg agtaatggca
     8161 gaacaatttt ttcattgtaa aaattgttca gttgttaatc tatgggattt cggtagcatg
     8221 ggcatttttt tatagaggaa tcacaccaac ctggccgttt tatctcttgt tagtgggaat
     8281 attagtagga aattttgccc ctcttcacta tcgtacgaac ttttttccaa ctgattctgg
     8341 cctttttctt aaaacaaaga cgcgaaaatt agaaaacatt attgaaatga caattacctg
     8401 tataatcatt ttcttagtta tattcctaaa cgtcttctaa taagaatctc ttaaaattag
     8461 cttgttacag ttagttacct agtttatata acacagttat cttatcttcc ttatcaacat
     8521 cttttgccaa ctctttgtct taaattcgaa acatataagc agagctacga acaccaagac
     8581 tgtttcggct ctgttttttg catcaaattc actcttatat agctgataat ctcggctctt
     8641 taaaagaaat tattttcgct tcattttatt ataaaaaggc taggaacctt gatgttatca
     8701 ggttcctagc ctttatttaa gtattacccc acagaccctt ccatatagat tttcaaatta
     8761 cacactagtt taagataaaa caaacaagga aaaacttacc atttactcaa aaaattaact
     8821 catataagta cattagaatg taaaatagac tggtcaaaat ctggtcaaaa aagtagatgt
     8881 gacacaagtt cgcattcaaa gcgatgatcc atgatttcgt aatggtcaat tatatggatc
     8941 aaagccacgc gacaagagcg caatggatgt ctctaccgat tggttgaata aacgaaataa
     9001 atagaatgtg aaaaaggatc ggacaaatgt tccagtttgt accgaccccc aaaagttaga
     9061 ccaaaaaatc taacttttgg aggtcggtat tttttatggc taagtatact tttgaactta
     9121 aattaaagat cgttcatgat tatttggatg gcaaaggcgg aagtgattat ctggcaaaga
     9181 agtattccat taaggctcca tcacaagtga agcgctggat caatgcctat caagaatttg
     9241 gtgaagaggg tctagtaaga aagcgacaaa agaaaaaata ttctgttcaa tttaagctgg
     9301 atgcgataga gttatatcta acaagtgagc tgtcctatcg tgaagttgcc aacacactta
     9361 acatgaacaa ccctaatttg atcgctagtt ggatgcgtca attccgtgag ggtgggatcg
     9421 atggactctc aaaaacgaaa ggatgtccac ctatcttgtc taagaaaaat gaacccaaaa
     9481 ataagaaaaa atctacaact aaagcaacgt ctaaagaacg tgaacgcatc gaggagttgg
     9541 agaaacgagt ccgttccctt caaattgaaa atgccttttt aaaagaattg aggaagctgc
     9601 gaaaacagga agcccaacaa cgacgaatga agcaatcgca cgaatcatcg caagcctccg
     9661 aggaccgttt aaattagttg agcttctaga aa
//
LOCUS       DACGLH010000099         9437 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.104, whole genome shotgun sequence.
ACCESSION   DACGLH010000099 DACGLH010000000
VERSION     DACGLH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.104"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            75..1379
                     /locus_tag="IXT21_002571"
     CDS             75..1379
                     /locus_tag="IXT21_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4869936.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1471..1836)
                     /locus_tag="IXT21_002572"
     CDS             complement(1471..1836)
                     /locus_tag="IXT21_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ4869937.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1833..2198)
                     /locus_tag="IXT21_002573"
     CDS             complement(1833..2198)
                     /locus_tag="IXT21_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ4869938.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2390..2638)
                     /locus_tag="IXT21_002574"
     CDS             complement(2390..2638)
                     /locus_tag="IXT21_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869939.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2637..2765
                     /locus_tag="IXT21_002575"
     CDS             2637..2765
                     /locus_tag="IXT21_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ4869940.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2822..4516
                     /locus_tag="IXT21_002576"
     CDS             2822..4516
                     /locus_tag="IXT21_002576"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ4869941.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4598..4789
                     /locus_tag="IXT21_002577"
     CDS             4598..4789
                     /locus_tag="IXT21_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869942.1"
                     /translation="MDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKVV"
     gene            5167..6300
                     /locus_tag="IXT21_002578"
     CDS             5167..6300
                     /locus_tag="IXT21_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ4869943.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6331..6651
                     /locus_tag="IXT21_002579"
     CDS             6331..6651
                     /locus_tag="IXT21_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869944.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6680..7144)
                     /locus_tag="IXT21_002580"
     CDS             complement(6680..7144)
                     /locus_tag="IXT21_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869945.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7273..8522)
                     /locus_tag="IXT21_002581"
                     /pseudo
     CDS             complement(7273..8522)
                     /locus_tag="IXT21_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8551..8868)
                     /locus_tag="IXT21_002582"
     CDS             complement(8551..8868)
                     /locus_tag="IXT21_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4869946.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8894..9214)
                     /locus_tag="IXT21_002583"
     CDS             complement(8894..9214)
                     /locus_tag="IXT21_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4869947.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3044 a         1860 c         1684 g         2849 t
ORIGIN      
        1 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
       61 tttgcaaaac cactttgctc aatgatatca ataacataaa aaaagaattt gaagaacaga
      121 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      181 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      241 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      301 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      361 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      421 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      481 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      541 aaatcttatt gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      601 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      661 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      721 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      781 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      841 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc attcgaacgt gcgctgattc
      901 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttc
      961 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1021 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1081 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1141 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1201 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1261 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1321 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1381 ggactgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1441 aatctcttga tacacatcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1501 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1561 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1621 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1681 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1741 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcaaa
     1801 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1861 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     1921 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     1981 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2041 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2101 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2161 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2221 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2281 tctaatgata acggttcgct taaatttttc tttttcactc tccccttttt ctccctccat
     2341 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2401 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2461 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2521 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2581 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2641 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2701 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2761 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2821 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     2881 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     2941 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3001 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3061 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3121 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3181 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3241 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3301 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3361 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3421 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3481 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3541 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3601 agatgaaatc actgtgactg gtttgaaaat gaccaataca ctgactcaag aagaagaaca
     3661 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3721 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3781 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3841 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     3901 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     3961 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctatcg tcgtgcatca
     4021 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4081 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4141 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4201 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4261 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4321 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4381 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta tccctatgtc
     4441 acgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4501 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4561 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4621 agcttttgaa caccgtttac caagaggttg tgaaaaagct gccctgcatc aagtaataag
     4681 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttggacttat taccgcagat
     4741 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa gtcgtttagt ttcgagtatg
     4801 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4861 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     4921 cattttttca acgtttactc tcttgccaat ccaacatcat ctaacagata caactatttc
     4981 aatgattacc attacactaa aaaatcggaa actcacctct ccgaacacaa atacataaga
     5041 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5101 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5161 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5221 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5281 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5341 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5401 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5461 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5521 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5581 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5641 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5701 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5761 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5821 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     5881 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     5941 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaaacgg cacaatctct
     6001 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6061 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6121 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgaaat gattgcagaa
     6181 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6241 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6301 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6361 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6421 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6481 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6541 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6601 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agatttgaaa
     6661 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6721 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     6781 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     6841 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     6901 gatttggtgt caaatcaggg cggttatgga agtcgttttc aataacgccc gctcctccga
     6961 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7021 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7081 acttcgcact ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7141 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7201 aaattagcaa tgacaacaat agtgatgtaa aaaaaacgga aaacaatcac ttgttctccg
     7261 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     7321 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     7381 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     7441 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac
     7501 cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa
     7561 catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat
     7621 atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc
     7681 tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct
     7741 atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat
     7801 caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa
     7861 tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac
     7921 tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa
     7981 agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc
     8041 cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa
     8101 gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc
     8161 taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc
     8221 ttttcggtag ccgttagcta aatgataagc aagaaagcaa tcaagacaga aagaatggca
     8281 aatgtaccgt tccaagcagc agccccgaat cctttccatg attcaccacc aaaaattgag
     8341 ttcatcaatt cttggaaccc gggaatcggt aagttattga tcattactgc taatgctcct
     8401 aagatcatta acggcatact caccataaat gagtcccgaa tcgcaactaa atgccgttgt
     8461 gcaccaattt ttgatgctac aggtataaaa tgtttctcca taaatgcaac aaatttatcc
     8521 ataaatcttc cctcttttct ttactgtaga ttacgcgttc aatcgacgat acagatcaat
     8581 gatctctttt gctaaatctg taaaagctat ggatgtcatc agatgatctt gactgtggac
     8641 tgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg
     8701 agagtgatgt gcttggacta aagattcctc tgcatctttg attttttcat ctgctaaagt
     8761 gaaatcacct tttttcgcag cagcaatcgc ttccattgca tcgctttttg catttccgcc
     8821 aaacatgatc agacccatga tggcttctag atttttttga tcttccattt attgtatcct
     8881 ccgataattt ttattatttg tttattagtt tttctgcttg gtcgagtact ttttcaccat
     8941 tcatcatgcc atagtcagcc atattgatta tatctaaagg aatgccttta ggtgctagct
     9001 tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc taaaagtaaa acgtctacat
     9061 ttttagattc tagttgatta tccgcatctg aagcagatac agcaaagatg tctgcttcca
     9121 ttccttttgc ttctgctgcc ttttgcattt ttgtgactag gagacttgta ctcattccag
     9181 ctgaacatac aagcatgatt gttttctttt ccattttaaa cacgttcctt tctatactta
     9241 cacttgtata taagcaagta gcgtgccaac tttgaaagcg ctttaaaaag ttgatatcat
     9301 cacatttaag gtctatacca agttgcatac acactaaaaa agaaagtgca cactaaacac
     9361 acaaattaat ttttattacc ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa
     9421 acttgtcaca acttttt
//
LOCUS       DACGLH010000100         9235 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.105, whole genome shotgun sequence.
ACCESSION   DACGLH010000100 DACGLH010000000
VERSION     DACGLH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.105"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..670
                     /locus_tag="IXT21_002584"
     CDS             <1..670
                     /locus_tag="IXT21_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ4869948.1"
                     /translation="SKMKISEVTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDF
                     FESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAE
                     SFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPT
                     ELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISG
                     YLDK"
     gene            737..1231
                     /locus_tag="IXT21_002585"
     CDS             737..1231
                     /locus_tag="IXT21_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4869949.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1287..1925
                     /locus_tag="IXT21_002586"
     CDS             1287..1925
                     /locus_tag="IXT21_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869950.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1997..2392
                     /locus_tag="IXT21_002587"
     CDS             1997..2392
                     /locus_tag="IXT21_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4869951.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2376..4829
                     /locus_tag="IXT21_002588"
     CDS             2376..4829
                     /locus_tag="IXT21_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4869952.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4826..6853
                     /locus_tag="IXT21_002589"
     CDS             4826..6853
                     /locus_tag="IXT21_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869953.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6850..7872
                     /locus_tag="IXT21_002590"
     CDS             6850..7872
                     /locus_tag="IXT21_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4869954.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7889..8818
                     /locus_tag="IXT21_002591"
     CDS             7889..8818
                     /locus_tag="IXT21_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4869955.1"
                     /translation="MIIKIERKEKKERPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            9063..9179
                     /locus_tag="IXT21_002592"
     CDS             9063..9179
                     /locus_tag="IXT21_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ4869956.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         3133 a         1536 c         1836 g         2730 t
ORIGIN      
        1 ttctaaaatg aaaatatcag aggtaacttt agatgagact gacgatttta cttattataa
       61 aggatataaa aagggctgga atacgctttg cacatatctt aaaataagag ttccttttga
      121 tgatttagac ttttttgaaa gtcataaaga tttgattacc cagacagcaa gctccatata
      181 tgataaacaa ggtgataatg tattagtgga tactatttta gtaccacttc cagagaatta
      241 cgaagtaatt aactttagtc agttgaaaat tagtgatgtg gtttcacaag ctattgaaga
      301 cgctgagtct tttatgagta atggtgaata tcaacgtgcc tttgacagag ttcacactgc
      361 ttttcatgga tatttaattg aaatactaaa aaaatatgaa attacagttc ctagagacga
      421 aaatctatct aaattatata gtaggataca acaattaatt gaaaaagaaa ttcaacctac
      481 tgaacttgct gatatagtga agacaacgat aaggtcatca aatggaatga ttagctctct
      541 taatgaagca agaaataggc attcacttgc acaccctaat acaaatatta ttggaaagag
      601 agaagcaaaa ctaataatag gtatttcaag tacagttaca gattatatta gcggatacct
      661 tgataagtag cgtcattaac tgattgctac tttttttatg actattttta taaaaaggat
      721 gtgataagaa ttggaaatga aagtctatgt ggcaaattta ggacggtata atgaaggtga
      781 attagtcgga gcatggttta ctccaccaat tgatgaggaa gaaatggcag aaagaatcgg
      841 tttaaatgag gactacgaag aatatgcaat acatgatttt gagcttcctt ttgatgtgga
      901 cgaatacacg ccgattagtg aaatcaaccg tctatgtgaa gccattcaag aaatcgaagg
      961 tacacctatt tataatgaac tcaaagaaat tcaaggcatg tggtttagta gcttggaaga
     1021 attactggaa aacaaagagg atattcattg ttatagtgat tgtgattcga tggaagatgt
     1081 cgcccgttat tacgtggaag aaacaggaca gcttggtgaa gtcccttcca atttacaaaa
     1141 ttatattgac taccaatctt tgggacgtga tatggaaata gagggtaatt acctagttac
     1201 ctctcatggg gtatttgaat attgtcagta agtttaacaa tccgtgttat aattaaatta
     1261 ataaatagca cggagaggta taaattatgg taaatgtatt tgaaaatctt aaactattaa
     1321 aactagacat gaaagataaa ggctgggtga tagattcctt ttactttcgt tacaaacagc
     1381 aaaattatat tgtacttgtt aagctatttg aaaaagagga aaaagttcca gaatatgctc
     1441 tgttaaaact agaattttta aaggaaaacg atttttcaga tatgctagct gtctatgcta
     1501 attcggtaaa attatataca gacacgaaaa caataagaga atattttggt atcgaataca
     1561 gtagtaactt gggagatgtt ttatttcaat ttagccaaac tcttgctcga tttattccga
     1621 cagaagttag tgagaagaaa aatgaagacc aaaaagaagc catgtgtttc tcattaagtc
     1681 aaagtgattc agaagatccc agaaaaaaat attgtttttc agttagaaga aatcctttaa
     1741 agggaaatgg tgagctggga aaacgaagtc cttttaatga caataaaacg agattgtata
     1801 gacccagctt atatgaacga ttagggtcag ataccaattt aagtttcaga tattcaatga
     1861 atccagatga tgaagaaact gatgaaggta ttattgcaaa atggacaaaa aataaaattg
     1921 aatagttaaa gcattgaaag agcaatcacc tattttgatt gttctttttc tatttttacg
     1981 gaaaggacgt gaagacatga aaaaaataag aagttacacc agtatttggt cagtagaaaa
     2041 agtgatttat gcgattaatg atttacaact ccctttccca attaccttta cgcaaatgac
     2101 atggtttatc gtttctttgt ttgtcgtcat actctttggc agtttaccac cactttctct
     2161 gattgacgga gcactactca aatacttagg gattcccatt gttctcactg ggttcatgtg
     2221 ccaaaagacc tttgacggaa agaaacccta tggatttcta aagtctatca ttacctatgc
     2281 tctacgccct aaagtaacct acgctggcaa acaaatacga ttaaaaaaag aaacaatgaa
     2341 tgaatcgatt acgattgtaa ggagtgaaca actagatggc atatccgatt aaatatatgg
     2401 aagataatct cgtatttaat catgatggcg aagtattcgc ttattatgaa ttaattccct
     2461 ataattacag ttttttaagt tccgatgaaa aaattcaagt tcatgataat ttctgtcaat
     2521 tgattgccca aaatcgtgat ggcaagattc atgctctgca actagcaacc gaatcaagta
     2581 ttcgagccac acaggaacaa tcgaaaaaag aaatcactgg acgattaaaa gagatagctt
     2641 acgaaaaaat agatgaacag accgaagcgt taatttctat gattggtgac aatcaagtag
     2701 actatcgctt ttttattggt tttaaactct acctcaatga acaggaagta tccgtaaaaa
     2761 atgttgggaa agaaattcaa aaagcatttg cggaattctt tcatggtgtg aatcacaaat
     2821 taatgggcga ttttgtttct atgcctatgg cggaaatcga acgcttttct aagatggaac
     2881 agttgttaga aagtaaatta tccagacgct ttcagattcg taaattggag aaagatgatt
     2941 ttggttatat tattgaacat ctttacgggc aaacaggtgt cccttttgat gactacgact
     3001 accatttacc gaaacgatac gaagataaaa aagtgttggt aaagaaatac gatattctca
     3061 aacccactcg ctgtttaatc gaagaaaatc aacgctattt acggattgaa caggaagata
     3121 ccactactta tgtggcgtat tttacaatta atagtattgt tggtgaattg gatttcccta
     3181 atagtgaaat tttttattac cagcaacagc agtttacttt ccccgttgat accagtatga
     3241 atgttgaaat tgtcgccaat aaaaaagcct tatctaccgt tcgcaataaa aagaaagaac
     3301 tgaaagattt agatagccac gcatgggaaa gcgacaacga aaccagtact aatgtggtgg
     3361 acgctttaga atctgtcgat gaattagaaa gtaatttaga ccaaaccaaa gaatctatgt
     3421 acaaactaag ctatgtggta cgggtatcag caaagaattt ggacgagctg aaacaacgtt
     3481 gtgatgaagt aaaagatttt tatgatgatc tgtctatcaa attagtacga ccttttggcg
     3541 atatgctagg cttgcataat gaatttattc cctcaagcaa gcgatatatc aatgactata
     3601 ttcaatatgt cacgtcggat tttttggctg gtcttggttt cggtgctaca caaatgattg
     3661 gtgaaaattc tggtatttac gtcggttaca atctcgatac aggcagaaat atttatctcc
     3721 aacctagcct tgcaagtcaa ggaattaaag gctctgtaac aaatgcatta gcgtccgctt
     3781 ttattggttc attaggtggc ggaaaatcat tctctaacaa tatgctggtc tattattcag
     3841 tactctttgg cggacaggca accattgttg acccaaaagc tgaacgtggt gaatggaaag
     3901 aaaccttacc tgatattgcc catgaaatca atattgttaa tctcacgagt gagaacgaca
     3961 ataaagggtt acttgaccct tatgtgattt taaaactccc gaaagattca gaaagccttg
     4021 ccattgatat tctcacgttc ttaacaggta tttccagccg agatagcgag aagtttcctg
     4081 tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca acgagggttg ttattggtga
     4141 ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag tattgctgac catatcgaaa
     4201 gctttgtcga ttatgatttt gcccatctgt tattcagtga tggcagtgtc acccaatcca
     4261 ttagcttgga aaaacaactc aatattattc aagtggcaga tttggtctta cccgattcca
     4321 atagctcttt tgaagaatac actaccatgg aattattaag tgtggcaatg ctgattgtca
     4381 ttagtacctt tgctttagac tttatccact ctgaccgttc catttttaaa attgttgacc
     4441 ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa agctctatct atgcgattgg
     4501 ttcgagcagg acgtgccatg aatgcagggg tctatttcgt tactcaaaac gcggacgatt
     4561 tactagatga aaagatgaag aataatattg gcttgaaatt tgcctttcgt tccactgacc
     4621 tagtggaaat caaaaagacc ttagaatttt ttggtgtgga caaggaagat gaaaacaatc
     4681 agaaacgctt acgtgaatta gaaaatggac aatgtttagt ttcagacatc tatggtcgtg
     4741 taggggttat gcagtttcac ccgatttttg aagagttgct tcatgccttt gataccagac
     4801 cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa aagaaatttc ttcactatag
     4861 ttggataacg ctcctttgtg tgggcgttat tttactttta ttaggcacgc tgggcaatgc
     4921 agtacaagcg acaggcttag ttgatgaaac cattgataca agcaatgaat actccaagta
     4981 tggtttgaat caatatcagc ttgattatta tgtggacaat agctggggct ggcttccatg
     5041 gaattggagt gatggcatag gacaatcggt gatgtatgga ttgtatgcca ttactaattt
     5101 tatttggaca atttctcttt atctatcgaa cgccacaggt tatttggtga aagaagccta
     5161 tgccttagat ttcattagtc aaacatccca agcaattggc gaaaacatgc agacgattgc
     5221 aggcattaca ccgaatggct tttccagttc agggttttat gtcggtttcc tccttctttt
     5281 tattctcatt gttggaatct atgttgccta tacaggattg gttaagcgag aaacgacgaa
     5341 agtgatacga gctgttttaa actttgtcgt ggtgtttatt ctatcggctt cctttattgc
     5401 gtatgcaccc gattatattg cgaaaatcaa tgatttttcc aaagatgtca gtaccgccag
     5461 tctgaatatt gggacaaaaa ttgttcttcc taacacggat agtcaaggga aagacagtgt
     5521 ggacatgata cgtgacagct tattctctgt tcaagtaaag caaccatggc tattgcttca
     5581 atatggctcc actgatgtag aagcgttagg ggaagaacgt gtggaaagtt tattgtctac
     5641 cagtccagat acaaacaatg gtgaagatag agaaaatgta gtgattgaag aaattgaaga
     5701 tcatgacaat acctatttaa ccctaccgaa gacgatttcc agattaggaa cagtcttctt
     5761 tttgtttgta ttcaatattg gtatctcgat ttttgttttc cttctaacag gaatcatgat
     5821 tttttcgcaa gtgctattta ttattttcgc tatgtttttg ccaatcagtt tcctattaag
     5881 tatgttgccg agttttgatg gcatgggcag gcatgccatt accaagttat ttaatgtgat
     5941 tatgacaagg gctggtatta ccttgattat taccgttgcc tttagtattt ccactatgct
     6001 ttattcgctg tcagcaagtt ctcccttttt catgattatg tttttacaaa ttgtgacctt
     6061 tgcagggatt tattttaaac tgggcgattt aatgagccta ttcactttac aaagcaacga
     6121 ctctcaaaac atgggcagac aagtgtttcg cagacctcgt caaatgatga atcgacaaac
     6181 cagaagattt caacgcagta tgagtaagat ttttcgtgga aaagcagggt caacgaagca
     6241 aaagacaatg acaacaaagc agaatcaaac caataaagct aaccatactc gtcaaaatga
     6301 acggactaca ccaaagaaag aaacagcact ttccaatgtg aaaaagcaac aaggcggtat
     6361 cgggcaacga ttaggtgaaa agacagccca tgtattggac acgaaaaatc gtctagtcaa
     6421 taaaagaaag caggcaaaac aacagataca ggcgacacca acccatgtca aatatgccct
     6481 tcatcaagga aaagagaatg tcactcataa tgtaacagac tttaaacaat ctatggcaga
     6541 taccaaagct caacgcaaac aacagcgtat gaaccagcag gagcaaaaac gaaagaacat
     6601 tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa agacgaaaag aaaacccatc
     6661 atctcctgtt cagaataacc agcagcatgt tcgcaagcgt cagcaaaccg tcacgcctgt
     6721 tttgtcacaa aacactaaac gtgaaaatca tcagcgacca ttgaaaaaag agcaaaaaca
     6781 aaagacacca ccgaagcaat tcaatcagag gaagctaaat aaaagaacgg ataagcgagg
     6841 acaacaaaca tgaagctaaa aataacagtg atttgtagtg tggtcttctc tctcttcttt
     6901 tttctcttat tattcctagt cattttcttt gcagatgata cggacagtgg cgaaaacaac
     6961 aaggattctt ctattccaca aggcggtgtg accgtttcac cagaagtgtt ggcacaccgt
     7021 ccgttgattg aaaaatatgg taaggaatat gggattgaag actatgtttc ttatatctta
     7081 gccattatgc aagtggaatc gggcggtaca gcagaagatg ttatgcaaag ttccgaatct
     7141 ttgggtttac cgcccaacag tttgagtacc gaagagtcca tcaaacaagg ggttaaatat
     7201 ttcagtgaat tattaaccag tgcagaacaa caaggagtgg atatagattc tgttatccaa
     7261 tcttataatt atggcggtgg ctttttaaat tatgtgagga gtcatgggaa aaaatatacc
     7321 tatgaattag ccgaacagtt ttctaaagag aagtcgggtg gtcaaaaagc agactatccc
     7381 aatcctatag ccatacctgt gaatggtggt tggcgatata actatggaaa tcagttttat
     7441 gttcagttgg tttcacaata cctaaccgac acttccccaa cagaatttga tgatgaaacc
     7501 gtgcaagtca ttatggacga agccttgaaa tacgaagggt ttccctatgt ctttggaggg
     7561 gcgtcgccta ccacctcttt tgattgtagt ggcttaatac aatgggtgta tgacaaagct
     7621 ggcatttctc tccctcgtgt cgcccaagat cagtatgacg ctacgcaaga aatctctatg
     7681 gaagaagccc aagcaggcga ccttatcttc tttcattcaa cctataatgc aggcacatat
     7741 gtgactcatg tggcgatata tttagaggga aatcgctttt atcatgcagg cgacccaatc
     7801 ggttatggcg acttaagcag tcgttattgg caagaccatc tgattggggc tagacgtgtt
     7861 attcataact aagaaaggaa tgaatcaaat gattataaaa atcgagcgaa aagaaaagaa
     7921 ggagagacca aagaaatcta aaaaaccgag caagcaaaga aaagttccga tgattaaagt
     7981 tggcacgcat aagaaattaa cctttgtttt atgggtttta ttgattggta gtgtcggatt
     8041 tggaatctat aaaaatttta ctgccattga cactcatact gtccgtgaaa cagaaatcat
     8101 taaacagcag attgtcgata ccaaccaagt agaaagtttt gtgaaatcct ttgcgaaaga
     8161 ctacttttcg tggcaacaat cccaagaagc cattgataaa cgaaatgaaa aattgaccca
     8221 ttatttgacg gaagaattgc aggtattaaa tgaagaaatg attcgtaagg atattcctac
     8281 tagttctagt gtgaacgata tacaggtgtg gcaggtttct caagtgaatg agaacacctt
     8341 tgaggtactg ttttctgttg aacaagttat taccgaagac aaagacaagg aaactatatc
     8401 gtctagcttt catgtggttg tccatataga tgaatcagat aatatggtta ttatcaaaaa
     8461 tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc tagaaagtga
     8521 ccatacagta gatacagaaa cgatggacga aatcactagc tttttagaaa cgttcttcca
     8581 gctctatcct accgccacag aaaaagaatt gacttactat gtaagtaatc atgtgttacc
     8641 tatgattaac aaggaatatg ttttcgagga attggtgaat cctatcttta ccagaaaaga
     8701 taaccaagta atcgtgaatg tcgctgtgaa atatttagat caagaaacaa aggcaaccca
     8761 aatctcgcaa tttgagttga tattagaaaa acaagataat tggaagattg taaaataaca
     8821 aatattggta catgattaca gatactttgt aatcatgtac tctttttgat aaaaaattgg
     8881 agattccttt acaaatatgc tcttacgtgc tattatttaa gtgactattt aaaaggagtt
     8941 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagcgg gaacaaataa
     9001 ttagatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc
     9061 atgtgattct aaagtatcca gagaatatct gtatgctttg tatacctatg gttatgcata
     9121 aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa
     9181 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgc
//
LOCUS       DACGLH010000101         8876 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.106, whole genome shotgun sequence.
ACCESSION   DACGLH010000101 DACGLH010000000
VERSION     DACGLH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.106"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..2289
                     /gene="fss3"
                     /locus_tag="IXT21_002593"
     CDS             <1..2289
                     /gene="fss3"
                     /locus_tag="IXT21_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ4869957.1"
                     /translation="TDKEGIATLDEIPHGTKVTITEKSVPAPYTIDTTPMTTTIKAGE
                     TIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSLAGNTFAIRKDSPTGEIVQEMTTD
                     ENGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELKYANQTVALVTSNVKG
                     QNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTLFTAKDGKAVKWSEAFKPELAKGT
                     KGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAPEGYSLDETKYPVSIKKVDDSEKN
                     AVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGFNDLTFKVSPLEGTNEITGAEDEA
                     TTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQKITPLEIRSTFKENKEDFVKSE
                     YVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNRLMLYDLPEEEDSLTSLATWKDGN
                     KELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDVDATKAAQEKDEKAKPVVIAETSA
                     TLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYENKEAFEAGDKPVAKDVSLNNQAQ
                     TVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFDDVSITHDVLDGSKEAFETILYAL
                     LPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDTGKYPEGTKFTFAEINYDKDGNIN
                     GKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVPSGSQESSPTVKTFPQTGEKNSNV
                     LLFIGFTLIFATAGYYFWNRRN"
     gene            2402..2716
                     /locus_tag="IXT21_002594"
     CDS             2402..2716
                     /locus_tag="IXT21_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4869958.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            2729..3103
                     /locus_tag="IXT21_002595"
     CDS             2729..3103
                     /locus_tag="IXT21_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4869959.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            3113..3448
                     /locus_tag="IXT21_002596"
     CDS             3113..3448
                     /locus_tag="IXT21_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ4869960.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            3531..4871
                     /locus_tag="IXT21_002597"
     CDS             3531..4871
                     /locus_tag="IXT21_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ4869961.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            4949..5623
                     /locus_tag="IXT21_002598"
     CDS             4949..5623
                     /locus_tag="IXT21_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4869962.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            5856..6290
                     /gene="dcm"
                     /locus_tag="IXT21_002599"
     CDS             5856..6290
                     /gene="dcm"
                     /locus_tag="IXT21_002599"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ4869963.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            6291..6998
                     /locus_tag="IXT21_002600"
     CDS             6291..6998
                     /locus_tag="IXT21_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ4869964.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            6988..7278
                     /locus_tag="IXT21_002601"
     CDS             6988..7278
                     /locus_tag="IXT21_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869965.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARMELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            7534..8718
                     /locus_tag="IXT21_002602"
     CDS             7534..8718
                     /locus_tag="IXT21_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ4869966.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            8733..8852
                     /locus_tag="IXT21_002603"
     CDS             8733..8852
                     /locus_tag="IXT21_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ4869967.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT         2918 a         1493 c         2037 g         2428 t
ORIGIN      
        1 actgacaaag aaggcattgc tacattggat gagattcctc atggtacaaa agtaaccatt
       61 accgaaaaat cagtaccggc gccttatacg attgatacca cccccatgac taccaccatt
      121 aaagcaggcg aaaccattta cgtgacttca aaaaacgcac gggaaaaagg gcaaatcatt
      181 ctggataaaa gcggagttga aacagggagt gatctttgga atgacaatta ttccttagct
      241 ggtaatacgt ttgccattcg caaagacagt ccaaccggtg aaatcgttca agaaatgacc
      301 acggatgaaa acggtcatgc ggaaacacca aaagagattg ctaatgcgtt ggaattggga
      361 acctattatg tgaccgaaac taaagctagt catgggttcg tgaatacgtt caaacctgta
      421 aaagtcgaat tgaagtatgc caatcaaacc gtagcccttg ttaccagtaa cgtaaaaggg
      481 caaaatcaag aagtcactgg cgaaaccact ttgacgaaag aagacaaaga tactggggat
      541 aaagcacaag gtaaggcagt ctttgagggt gctgaataca ccttgtttac tgctaaggat
      601 ggaaaagctg ttaaatggag tgaggctttt aaaccagagt tagcgaaggg aacgaaaggt
      661 tctgatgaaa cggtcacttt gactttagac gaaaagaacc aagttgccgt taaacattta
      721 gctatcaacg agtattattg gcaagaaaca aaagcgcccg aaggatatag ccttgatgaa
      781 acaaagtatc ctgtatccat caaaaaggtg gatgatagcg aaaaaaatgc cgtgattacc
      841 cgaaatgtta cggcaaaaga acaagtgatt cgctttggct ttgatttctt taaatttgct
      901 ggatcagctg cgggcactac cgaaactggc ttcaatgatc tgacctttaa agtgtcgcca
      961 ttggaaggga ccaatgaaat cacaggtgcc gaagatgaag cgaccacagc ttataacgag
     1021 caattagggt ttgatggtta tggcaagttt gaaaatcttc cttatgggga ttatttactt
     1081 gaagaagtag aagccccaga aggttttcaa aagattacgc cattagaaat ccgttctact
     1141 tttaaggaaa acaaagaaga ctttgtgaag agtgagtatg tctttaccat taccgaacaa
     1201 gaccaaaaac aaccaattaa aacggtgacc gttccttacg agaagctgac gaacaaagca
     1261 ttttctgtta gtttgaaccg tttaatgctc tatgatttgc ctgaggaaga agatagttta
     1321 acttctcttg cgacttggaa agacggaaac aaagaattaa cttctctgga ttctaccgag
     1381 ctagttgata aattgagtta caatctacat gaaatcaaag aagactggta tgtggtagcg
     1441 caagccattg atgtggatgc gacaaaagct gcccaagaaa aagatgaaaa agccaaacca
     1501 gtggtgattg cagaaacaag cgcaacgttg gcgaacaaag agaaaacagg aacttggaaa
     1561 attcagcata aattaaccgc cgaacaagtt ttaaataaaa ctattgtctt attcaactat
     1621 gtgtatgaaa ataaggaagc ttttgaagca ggcgataagc cagtagcgaa ggatgttagc
     1681 ttgaataacc aagcccaaac tgtcagttgt acagtggaac accatgtttc tatccaaaca
     1741 aaggcccacc tagaaaacgg ttctcagacc tttactcatg gtgatgtggt ggatatgttt
     1801 gatgatgtgt cgattactca tgatgtactg gatggctcaa aagaagcttt cgaaacaatt
     1861 ctgtatgctt tattaccgga tggtacaaac aaagaaattt ggaaatctgg caagattgat
     1921 tatgaagtaa atgataaaga attcaccaaa actgtgcttg cgaaaaaagt agataccgga
     1981 aaatatccag aaggaaccaa gtttactttt gcggaaatca attacgataa agatgggaat
     2041 atcaatggaa aacacaatga agatttgaaa gaaaaatctc aaaccttaac accaaaagaa
     2101 gttccaacca tactaagtac gccgaaacaa ccggaaacac caactgttcc aagtggctct
     2161 caagaatcta gtcccacagt gaagaccttc cctcaaaccg gggagaaaaa ttccaatgtt
     2221 ttactgttca ttggttttac tttgattttt gcgacggcgg gctattattt ctggaatcgc
     2281 cggaactaag gtgatgagat gatacaagta aatcctaaga accaacgtgc gcgaccgcct
     2341 cctaaaaaag tagtcgcgct atttttgtat ccaaaactaa caatgaaaag aggaaaagaa
     2401 gatggaattg aaatttgtcg tgccagatat ggctgaaacc tttggaaagt taagttatgc
     2461 cggtgaagga gaaattataa cagaaggata tggacggaat actacggtga ttggtcgcag
     2521 ttatcatttg tattccagta agcaacgggc cgatgatatt gaggtggtag tagctgcgga
     2581 agccggtgaa aaggattttg atcaagacca accgctaaaa gccgtgaatc cccatttggt
     2641 tgccaaaggc tatgagattg agaatcgtgg gtttactgat tacgtgttgt atgttgatga
     2701 tttagtgaaa gcataggagg aaaagaagat gagattaaca gaaggtattg tcgtagattc
     2761 aggtttaaca tttgggaagt tgcgtttttc cgcattacgc cgagaagtac ggaagcaaaa
     2821 tgaggatgga acaattggta atgaggtaaa agaacgaacc tataatttaa aatcctctgc
     2881 acaagggcga atgattcaag ttagtatccc agctaatgta cccttacgcg agtttgctta
     2941 tgatgcagag gtagagttgg tcaatccaat cgtggatacc gttgccaatt atgtttttcg
     3001 agaaggaact accgttaatt ggttcattaa ggcggatgat ttagtcttga agcggcagcc
     3061 gaatcaagga aatcctacga accaaaatga aagaaagaaa taggtgaaga ttatggaaat
     3121 taatgttgaa catattttag attgtctcga tcaatatgga aaaggcgagc tgactgagga
     3181 acaactcgtt aaagcattaa cctctgatga aaaaatgttt ctgattatac atcaaggttt
     3241 acttggagaa aatagcgaca gtgaagaaga ctctgatgta ctagactggt taggagaaga
     3301 agagtctttc tttatagttg tcgaagtaaa tgaaaactta tgtcggcagg cagaatccat
     3361 tttggaagaa attggtgtgg agatgccgga tgctattgaa ggctttttaa atcaattggt
     3421 tgaaacgaaa caactcccct cctcgtagca gtaaatgact agcataactt tttgaaattg
     3481 cctaatgaaa tcctattggg ctttttcgat tggaggtgga aaccatcaaa atgtacaaag
     3541 gacatcgcat acgcgcagga gatcagcact tggtttatca ctttgttctt ggctggctac
     3601 tcgctctgtt catcggttgg atgagcgtct tttattttca agagtttaga caatttgata
     3661 tttctaagct atcactttct actattgaaa tcgtttggtc tataaaagat ttagtatgtc
     3721 tgcttggaag tctcgctttt tcaggagcta tgatattgct ttatattcac ttttttcttg
     3781 atcattggcg aagcttgtgg catcgacaaa agctagctcg gatgattcta gagaaccatt
     3841 ggtatgaagt gaaacaaact caaagtgaag gctttttcaa agatttgaat agtagccgaa
     3901 ccagagagac tatcagttac ttccccaaaa tctattatcg aatgaaagat ggattactgt
     3961 ctattcgcgt tcaaatttca cttgggaaat accaagatca gcttttaaaa ttggaaaaga
     4021 agttagaaag cggactgtat tgtgagttag tagaaaagga actcaaagac tcctatgtgg
     4081 agtatacttt actgtatgat atgatcgcca atcgaattgg gatagacgaa gtagtggcag
     4141 aaaatgggac tctgcgattg atgaaaaatc aagtatgggc ttatgattct ttaccccata
     4201 tgttaatcgc tggtggcacg ggtggaggga agacctattt cctcctcacc attatcgaag
     4261 cactattgaa gtcagatgca gaattgttta tcctcgatcc caaaaatgca gatttagctg
     4321 atttaggtac ggtgatgcct catgtttatt cacaaaagga agaaatttct gcttgtgtgg
     4381 aagatttcta tgaacgtatg atggctcgta gcaaggcaat gaaagaaatg tctaactaca
     4441 aaacaggaga gaattatgcc tatcttggac ttccacccaa ctttttaatc tttgatgaat
     4501 acgtggctta tatggagatg ttaacgacga aagaaagtgc ggtgattttg aataagctca
     4561 aacaaatcgt gatgttgggt cgtcaatctg gtttctttct gattttggct tgccaaaggc
     4621 cggatgcgaa atatttagga gacggaatcc gcgatcaatt caactttcgc gtggctttgg
     4681 gtcgtatgag tgaactcgga tattccatga tgtttgggga ggtcgaaaaa aacttcttta
     4741 tgaagcacat caaaggtcga ggttatgtgg atacaggtgg cagtgtgatt agtgagtttt
     4801 atacgccact tgtaccaaaa gggtatgatt tcttagattc aattagtaaa ttagaaaaga
     4861 tgaatgattg agtgatcttc aatactgact ttatacttca agtaacgtat aatactttta
     4921 tatagtagga agaaagggga taattaatat ggaaagtaat gattcttatt tagagtatgc
     4981 aaaagcaaat aaggataagt ttatttctta tattatcaaa gggaaatccc ccgaagttgg
     5041 acgagacgca gtttttatgg cagggagtcc tggggctgga aaatcagagg ttgccattgg
     5101 attagctgag aattatgata accatgtgat tattgatgcg gattactttc gcacccagtt
     5161 tcctgattat aatggaaaaa actctagtct ctttcaaaaa gcatcttctt ggttggtaga
     5221 gcaggcattt agatatgtct tagatcatgg ctattctttt attctggatg caacttttgc
     5281 aatattaagt gctgaaaaaa atatctcgcg ggcgttgaaa aaacattttc gagtgacgat
     5341 tttctatgtt tatcaagacc ctaaaatcgc ttggaaattt acacaagaac gtgaaattgc
     5401 agagggacgt catgtgccga aaaaggcatt tattaacgct tttttcaaat caagagagaa
     5461 cattgcaaaa gtaaagagta aacatccaga tgtattgctt catatcataa taaaagatta
     5521 tacgaataat atttccgaag ttcattatgc tacggataat ctagaattat ttttaccact
     5581 agggtatagt aggaaagatt tggaggttca attacatgac tgagaaagaa caagtaacga
     5641 aaattgtaaa gaaatacaat aagagcattg cggatttatc ggaaaatgct actgcaaaag
     5701 agttcaaaac ggtgattaaa tatgtagccg atcaggccaa tgaaaaacaa cggaaattag
     5761 ttggattaaa taaaaaatag taggacaaat taataatgta tgagccaaga gcaaaatgtt
     5821 cttggctttt ttctttgcaa tgaatgaggt aatgtatgag atttttagat ttattcgccg
     5881 gtattggtgg ctttcgttta ggtatggaac aggcgagcca tcattgtatc ggtttttgtg
     5941 aaattgataa atttgctaga cgtagttata aagcaattca tgatacaagt aaggaggtgg
     6001 aaatgcatga catcacaagt gtatcagatg agtttattca atccctcgga ccagtggaca
     6061 tcctttgcgg cggatttccg tgccaagctt tttcaattgc gggaaagcgg caaagatttt
     6121 ccgatactcg aggtacttta ttctttgaaa tcgctcggtt caccactctt ctccgaccta
     6181 agcttttatt ccttgagaac gtccgaggat tgctcaatca cgaaggaggg gctacgttcg
     6241 agacgatcct ccgaacgctg gatggattgg ggtatgacgt ggagtggtag gtgcttaact
     6301 caaaggccta cattccccaa agccgggagc ggatattcct tatcggacat tctcgagacg
     6361 catgtaccga acaagtattt cctatcattg gaccgtcttc aacatctgat caaaacatca
     6421 gaaatttggt aaatatcaat ccttccaatc gaggaatggg gggacaagtc tatggttcag
     6481 atggcgtagc gccaacctta acaggtgatg aaggaatcaa aatcgcactg ccagcaaggg
     6541 atggaatctc tgtagcagga ctattaccgg gaaagtttga acaaggtaat cgagtttatg
     6601 aagttactgg aaccgccccc accttgtcaa cgaaacaagg tggtacaaaa atcatgattc
     6661 gaaaaaatga aacagaatat caagaggtga agccagaaga cagtgtgaat ctggcttttc
     6721 ctaattccac aagtcgccga ggtcgtctgg gaaaacagag tgttcatacc ttactaacag
     6781 gagaccaaca agcgatcgtc acggatcagt atcaaattcg gaagctaaca cctagagagt
     6841 gttggcgact tcagggcttt cccgattggg cttttgatcg agattctcaa gtcaattcag
     6901 atagtcagtt gtataagcaa gccggaaact ctgtaacagt tccggtcatt tttgatattg
     6961 ccagaagatt aaaagagttg gatgtacatg gtttatgatg agttgattgg agaaatttat
     7021 tgggtgattg gaaagattca gtcagaccca gaattagaag aacaattgcg acggttaaaa
     7081 tttgatattc gcaagaatgg tgtcaaagtc gctggcgatc catatttaat gaatgaggag
     7141 acggatgctc gaatggagct gaatcaagtc atcagtgaat tggaacgaat tgcggacttt
     7201 gctaaagaac ctgatatccg ccaatacttg tttgagataa aagcagagtt ggaaattgag
     7261 ggtattaccg cggagtaacg gccaaataaa ccactgatag gcactgaccg cgggggattt
     7321 gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg acggcgttag ccggccatga
     7381 tgtctcaccc cccgtatcta acaggggggt acaaaaacac aaaaaataag caagcaacga
     7441 taaggaatgg ctgtcctatc aaggataata agcaatttgt gtagatgtta actgatttga
     7501 aaaagttaac accttaaatt tggaggtgag aacttggcac aaaggaattt agattatcgg
     7561 ctattaaaag accggcgtaa tgaatatggc gtttcacaaa ataaattagc tacgacttgt
     7621 ggacttagtc gcccgtactt aaatcagata gaaaatggtg gcgtaaccgc atctataaaa
     7681 accatgagga aaatttttga ccaactggaa agtttcaatc ccgagttacc tttaacgtta
     7741 ctgtttgact atgtaaggat tcgctttccc acaacggatg cacgaaaaat tattcaagaa
     7801 attctccatt tgaaatttga ttatatgctc catgaagatt atgcctttta ctcctatcaa
     7861 gaacagtatg tcatgggtga tattgtggtg atgttgtctc atgaagaaga taaaggcgtc
     7921 cttttagaat taaagggtcg tggttgtcgg cagtttgaaa cttttttact cgcccaaaag
     7981 cgtagctggt atgacttttt cgaagattgt ctaaaagccg gtggcgtgat gaaacgctta
     8041 gacctggcaa tcaatgatcg agttggcctg ttagatattc ctgatttaac gaaaaaatgt
     8101 cagaaggaag aatgtatctc cttgtttcgt accttcaaaa gctatcgttc cggggaactg
     8161 ttgaaacctg atgataaaga tggtatgggc aatactttgt atattggcag tcttaagagt
     8221 gaggtctatt tctgtttgta cgaaaaagac tatgagcaat atatcaagtt agggattcca
     8281 ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac tgaaaaatga tcgagcttat
     8341 catgcaattc aagatttgtt aaaaggtcgc agtattgaaa gtaccacatt ttccattatt
     8401 aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa ggcgaactaa ctggcccttg
     8461 aatgaacaat gggggcgctt cattggacgg aatcagaagg aaattcaatt aacttctgaa
     8521 ccgaagcctt ataccattga acgaacgttg aactggcttg gacgacaagt cgcccctacg
     8581 tggaagatgg ccaaggagtt ggatcggttg aatcagacaa cgtacattca ggatatggta
     8641 aataatactc gactatcaga ccggcacaaa aagattttgg agcaacaaag tatggcaatt
     8701 gaaaatttga ttatatgaaa agaggaatcg tgatggaaat tgtgaaagat attttattgg
     8761 tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgcatgca agtgcagcgg
     8821 cggaccgagc aatggaaaag aaaggaaagt gatgaaatga atttcggtca aaattt
//
LOCUS       DACGLH010000102         8491 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.107, whole genome shotgun sequence.
ACCESSION   DACGLH010000102 DACGLH010000000
VERSION     DACGLH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.107"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..174)
                     /locus_tag="IXT21_002604"
     CDS             complement(<1..174)
                     /locus_tag="IXT21_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4869968.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFLM"
     gene            complement(594..1715)
                     /locus_tag="IXT21_002605"
     CDS             complement(594..1715)
                     /locus_tag="IXT21_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ4869969.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2043..3452
                     /locus_tag="IXT21_002606"
     CDS             2043..3452
                     /locus_tag="IXT21_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ4869970.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3449..4177
                     /locus_tag="IXT21_002607"
     CDS             3449..4177
                     /locus_tag="IXT21_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4869971.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4305..5216)
                     /locus_tag="IXT21_002608"
     CDS             complement(4305..5216)
                     /locus_tag="IXT21_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4869972.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5233..6240)
                     /locus_tag="IXT21_002609"
     CDS             complement(5233..6240)
                     /locus_tag="IXT21_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ4869973.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6237..8366)
                     /locus_tag="IXT21_002610"
     CDS             complement(6237..8366)
                     /locus_tag="IXT21_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4869974.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8371..8478)
                     /locus_tag="IXT21_002611"
     CDS             complement(8371..8478)
                     /locus_tag="IXT21_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869975.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1729 c         1486 g         2677 t
ORIGIN      
        1 cattaaaaag ttagtaaata attgccctct aagttccacg gtctgttcat taataacttt
       61 atatcctcgt ttttcaaaaa atggctttgc ggtaatggaa gcgtgggtag tgattttttg
      121 cataccagtt tctaaaacat ggttttccaa ttcatctgca attttagtcg caataccttt
      181 tccttgatgg ctatgttttt gtcacaactt cagtattcct gtaacaatac ggatttcagt
      241 atacaacaac agcttttgaa aagatacttg caaaaggatt aagatatcta tgacctgcta
      301 tgctcactga tgttgaaaag aatgttttat taggattgag gtaagcaaga tagtgaatat
      361 gtccggaaag cctgtaaaat aaggcttttc ggacatatgt gaagttataa aaagatagcg
      421 aaaaatctat ttagtatcac taatgttgcg aacatatcgt cagaatagtc tgacatttat
      481 ttcttgctaa aaatttcaaa aaagtcaagt gctgacgcag cggatgacta ttttccagaa
      541 aattttagta tgtattcaaa aatcaaacgt gacaacagct attaactaca tcctcatcca
      601 ggtctgtaac gtttgaacca ctcttctgcg ggctcaaaca ttaagtaatg gaaggtacgt
      661 ttcaattgaa acagcgtcgc tgtcgtattc aactcaattt ttatcacata agtgagaagc
      721 gttgcgatta aggtgaggat gacttgattt atcgcccctt gttcactatg tgaatatagt
      781 cgtttaatgg tcagattttg ttttaagtgt ttgaacaaca attcaatctg ccaacgggct
      841 tgatacatgt cggccacttc gtttggcgat acatcaaaga gattggtcac cattcgcagg
      901 attttttgtc ccttggtaag aaccgttacc aacctaaaac gctctgtcac atggttgaca
      961 ccgccgacaa gaatcacttg ttggtcactg attatcgtcc catcacgggt tgttgtatct
     1021 gctacttcaa tttgatccaa gatggtaatt tttgtattag attttgtccg tgtaacgaag
     1081 aaatagccgt cacgatttag tttgtctaac aacttgtaat caaagtagcc gcgatccacc
     1141 acatacgttg cttgcgtttt atcaacaaga acttcaaggt gattacggtc atgctcactc
     1201 gcatttgtca tcgtaaaact ttcaggatac tgattggttt tatcaagata acagagattc
     1261 aaatgtagct tgatgccaga ctttgttttc cgaaacttgg cccatggaaa ccatgctttg
     1321 ttcaacggaa tagtcgttga atcaatcaat tgtagactgg ttgtttttga gcttggctgc
     1381 tgagcagaaa ttctggcgac taattgcgta aaaatttcca tcaatacttc ttgcttgatt
     1441 tctggcgctc tacgagataa cgagctgtaa cacagggaat caatgccaag ttccttgcag
     1501 aggcgctgat ctaaaaaggc gcgatcaatc tcgcgatagc ttggcaattc ttcataaacc
     1561 gcatgaagta acactttaag cgtcgtttgg aagtctagtt ttttactgta gaaatcaaag
     1621 tcgctaatta agctttgaga gttttctgat aaaccagaaa aatttattgc agaaaaccat
     1681 ttttgaaatg aggtttttac gttatactta tccatgcgtg agtccttttt attggtcttg
     1741 gataagtcat ccgagaatag tataaaggac ttttttgcgt ttggatagct ttgtattagg
     1801 cttttgatgg gttaaaaagt ctttgcaaca ctagtaattt agtatttatg tataaattga
     1861 aagatatttt accaccactt agtcttctta aaagtgatcc aacattttgt ttgtggcaga
     1921 atttaactac acactaatgc agccgaaagt acacaactat gcagcagttt gactgcgaac
     1981 tggtttcctt ccatcataag tttgaatgac ttagtgatgg aggtacagaa aggttgttaa
     2041 ctttggatga tattaaaaca ataaaacatt tgagaaataa tcgtgatcag tcaattgatc
     2101 agataagaaa agcaatgaat atcaattggc gaacagcgaa aaaatacgcc gatagtgatc
     2161 aactattcat tacagatccc actcgaaaaa acaaatccat gatggatgat ggttggggag
     2221 aaatcatcgg tagttggcta gaagaagacc tacgtttacg cagaaaactt cgtagaacgg
     2281 ccaaagctat ccatgaacaa ttgaaacctt tgggtttccc tggctcctat cgaacagttt
     2341 gtgtatacat ttctcaatgg aaaaagcagc gttacgacga aacgaattac caagctgtcg
     2401 atcgtcttga tcatccgatc ggagaagcac aggtcgattt tggaacaatg gaagttatgc
     2461 atgaagatca catgaaagat atacaacttc ttatcatgtc atttccctat agtaatgcga
     2521 cgtccgcaag tgccatgcca gctcaaaacc aagagtgttt cctggaagca ttaaaacaac
     2581 tatttaatca atgcggcggc gttccaaaaa cgatcagaat agataactta actccagcgg
     2641 tgaaaaaaac aaaatcaaaa tttgaagaag cacaactaac cgatgaattc ttacgtttca
     2701 ccattcatta tggttgtgat gttcaagtct gtaacccacg tagtgggcat gaaaaaggac
     2761 atgtcgaaaa taaagtgggt tatgtccgat acaatttctt ctcgacttcg ccaatcatgg
     2821 actcttttga gtccctaaat aagcaacttg aagaacaatt aaaagcagat cgaaaaagga
     2881 cgcattattc aaaacatcaa ttaatcgaag atttgtggaa gaaagaaaat aaagcgttac
     2941 attctcttcc acaagaggat tacccaattt ttaaagaagt ggaagctaaa ctaaatcgtt
     3001 atcacgaaat gaaattggat caaacggtta ttcatattcc taaagcatat ggctattctc
     3061 aattgactgt tgttttgact tggaataaat tcaaagttct tacgcctcat ggagaaatat
     3121 taatgaacga atatcgcccc tatatgaaca aaaaaagatt gatcccatgg agtgacatca
     3181 tgaaacaatg gagtaataaa ttgaatgtcg ttagttattc gaggtattgg aagtacttgc
     3241 ctgcacgcct tcaaaattat ctagcgattg ataataatca cctgaaatac agacggataa
     3301 aacaattact aagtttatta gcgacctacg atatccaaca aattaatgaa caattttacg
     3361 aattagtcgt tcaagaagac gccccctctg actttgatgt gaattggtca aactatgatg
     3421 cattaacaaa aacaggaggt gtgcaaaaat gattggacag acgttagaag attattgtaa
     3481 aaagcttcga ttaagccata tcatcacgaa cataccaaga gcttctttcg aagctccaga
     3541 acaatttatt accgatttat tccgtttaga actggagacc agacagcaga tcaaattaga
     3601 acggatggaa aaattagcca aatttcctgt accaaaatcg ctaaagaact atgaatggca
     3661 cgaagatatt tttctaccat ctcatctaga taaggagacc cttgtctctc ttgattttct
     3721 aaaaaggcaa gaaaacattg tttgtgttgg ctctcctggt acagggaaaa cacatctagc
     3781 gatcgcatta gggagaaaag cctgtagtga aggtgtttca accaaatttt tccgagttaa
     3841 caatctcgta gagcaattag agaatgcatt aaaaaaacaa cagctagcca gtttcaagaa
     3901 gcgattcgaa acaacagaat taattattct tgatgaaatg gggtacttac cattgacaaa
     3961 agaaggagct gaacttctct tccaactgat ttcagattgg tatgagcaaa agagcattat
     4021 tattacctct aatttagaat tcagtcaatg gaatcgagtg tttatggatt ctcgattaac
     4081 agctgctctc gttgatcggc taatccatca cgctcacatt atcagcttca aaggcgaaag
     4141 tttcagattg aaaaattcat tatctaaatc atattgaagg aaaaaactag ttgcagcaaa
     4201 actctgcact ttcggctgca tttttttgta aaaagctatt gcacaacaca aacatttgaa
     4261 cgacgtaatt ccaaatgttg gattgctttt agataatctt ttatttattc acaattgcta
     4321 atagtccaat tttcattttt gtatagtttt aatctatact gaaaaacact tgttgttttg
     4381 gacaactcat caagatactc aacaccaaca atcacttgaa tcgctttacc cttttgtttg
     4441 taaataggat tcactaattc cacaaatttc aaattagtat taattggttg catcgcgtct
     4501 ttttcaacat aatgctctag ttctttttct gaggctgctg gataaagcct gaaaaacgtc
     4561 tctaaaaact cggtgatttc ttccgttgtc tttgaatcaa tttttgaatc actttttact
     4621 tgtcttactt tatgttttgc cttttctggc ttaccaatca tagtgggtaa actcgttaca
     4681 atatgattgc ctttttcatc ttcaaagatc atcagacgat atgcagaaga aattgattgt
     4741 gtctttttcc cttctgtaat cttttgatct accacaaatg aaacgacaaa ttcttccgag
     4801 tgtttttcaa catctagaat ttttactgat acaacctcgg aactagtggg aatatccgct
     4861 cgaaccatat cttggctcaa agcgagccct tcttctgtaa ggtactgctc aagattagtc
     4921 attctctttt caattacctc cttgttattc ttccatgaaa aatattcttt cacaaaatct
     4981 acagtaaaat tttctactcc agaggtattc actattttct ctttcacgac cactttctca
     5041 tgagttgtat gctgatcaat ggccgtgaag tttttgtaga ttccaaaagc aaggctacaa
     5101 gacaataaaa gccaaagggc aagcaccatt tttcgatgct tgcccacgga tactactcgt
     5161 tctttcttct tttttggaat cttttccttg ttacttcgct caatttttat cttcattttc
     5221 cattcctctt ttctattgtt tataccgacc agcaccaata atgtgctgtt gccaatagct
     5281 atcggtaaga ttggtccaac ccaccggatc tcctgcatga aacattctgt tctctcctgc
     5341 ataaatgccc acatgagtca cataagtacc ggcatcataa gttgaatgaa agaaaatcaa
     5401 atcgcctggt ttggcatctt ttaagtcaat atgagtcata gcatcatatt gatcctgagc
     5461 cacccgaggg agtttcaaac caactttggc ataacaccat tgagttaaac cactacaatc
     5521 aaacccagtt ccgggacttg acccaccaaa tacataagcc gttccttcat acttgaacgc
     5581 ctcgtcaaaa attccttgga ctgttttatc atcaaactgt gttgtagtta ggtactgctt
     5641 cactaataag caataaaaca tattgccata gttataccgc cacccactat tttccttaat
     5701 cgcgatggga ttgggataat cgactttttg accacccgat ttttcttttg aaaaagattc
     5761 ggctaaagca aaactatgct tctttccgtt tttcgcgaca taatccaaat acccgtcacc
     5821 atagttataa gcctgaatga tactatcttg atcgcatcct agttctttgg aacacttaac
     5881 caaagacgaa aaatagagac acacttgttt gatggatgcc tcggtactta atgaattcgg
     5941 tgataaacct gctgattcag aactttgcat gacatcttcc attgtacctc cagattcgac
     6001 ttgaataatc gccaaaagaa tcgaaacata ctctgttata tctgattctt tggcatactt
     6061 ttccaccatt gaacggtgtt tcaaaactac ttctgagaca ctactacctc caggagaagt
     6121 tccagaacta ccacttccct caccgtcctc accaaacaac aaacccacac acatcaagat
     6181 gcccacaaaa gagatggtca ggaaggttaa cgaaataata ctcaattttt ttaagttcat
     6241 cttttctcac ctggctttgg ttttgggcga gtgttctttt ttctttgtac ttgttgattg
     6301 gttcgctttg gaaaagtgaa tctctgtttc acttgttgaa aggaattgtt cttgcttagt
     6361 ggctgagtgc ttccgtttgg aatgacttct tgactagaaa gtttccgttt gatttcagga
     6421 ttcgaatgta cttgtaccct tggttttaaa gacctatttt ctggtagttc atttttctgt
     6481 ggttttatct ccttttttat cggaacattt tcccttcctt tctcatgacg attccatgct
     6541 tcgttcaata ctttgcgttt ttcatccatt tttgcgtctc ttcgttgtcg ttgtttttct
     6601 cgcaactccc ctcgatttgc tttttcttcg aaaggccctc gcttaaagtc ctctggtact
     6661 tttttcgtct tttccatgcc tttatgaagg ctgtatttca agttagttgg tgcatcggtt
     6721 acttgatttt ttgcttgttt cgctttgtcc ttcacgattt cttttgtatc gagtactttt
     6781 ccaacttttc taccaactaa atttattcgt gattctttct tcgtgcccac tggttgttct
     6841 tgttttttca gatcagaatt tagctcgtta atgcttctcg cggaatggtt ctttctattg
     6901 gacactctat tgatttttga actacctttc ccttttgtag aactggtagc tagtttccca
     6961 ccaactgctg caccgccacc aaaagcaaaa gttcgaccca catttcgatt aagctgtcgc
     7021 aattttcgat tcatcaacat ttgcggtttc cgcatgactc gccttcccat tgactgacta
     7081 tcattacttt gaaggttaaa catgctcata atgtcaccta atttgaaata gataccggca
     7141 aaggtcacaa tttgtagaaa agctaccata aagaagggat aggtggccga aatattgaaa
     7201 aacatcgtgg agatactgaa tgcagtcgta atgactaagg taactcccgc tcgcatcata
     7261 atcgtattga acaaacgaat aatcgccctt ttgcccaaac tctcatacgt cggcaacatc
     7321 gacaataaga aactaatggg cagaaacatc gcaaagataa tgaaaaggat ttgtgagaac
     7381 aacataatcc ctgtcagaag aaacacaaaa aaggaaatcc caatattaaa caacactaaa
     7441 aagaccacgg ttcctaaacg gttcatggtc ttggtaatac tgagattcgc attgtcatta
     7501 tcttcaatct ctgctttgac tgcttcttcc cgatccttac ccttattttc atccggactc
     7561 acggataaaa tcttattaac ccgaccctcg ccaatttctt ctatattgga atcatcgaat
     7621 tgtaatagta accagggttg ttgaacttga atcgaaaata aactgtctcg aatcaaatcc
     7681 acactatctt ttccctggct ttctgaatta ggtacaacaa tcttggttcc aaggctaaga
     7741 gccgcttcac taacgtctga gctaaagtca ttgatttttg taatataggt gggcgcatac
     7801 gcaatgaaag aacccgagag tagaaaaatc atcacaaaat taatgacggt tcggactgct
     7861 ttggtagttt ctcgttttaa taatccggta tacgccacat aaactcctag agctaaaatc
     7921 atcaataata ggaacccaaa gtagaatccc gaagtctgta atccattttc agtgataccg
     7981 gctaaagttt ggatattctt tccaatactt ttagccgtat ccgaaatgaa atccaacttg
     8041 taagcttctt gaaccacata gcccgtggcg tttgataaat acagacttac tgtccaaata
     8101 aaattggtaa tggcataaag accatacatc acgcttttcc ctaagccatc cccccaattc
     8161 caaggtaacc aatcccaaga actatccaca aagaaatcca gttgataatt gttcagagaa
     8221 tatttggaat aaaggtttcc ggcttcaact gtatcatcaa ccagacctgt cgcctcagct
     8281 accgtaccga ctaaactcaa tagaactgtc agagtgacga tgctaataaa aacgattccc
     8341 acaatccgaa aaagtttctt tttcatcccc tcactccatt ctctcttgaa ccggcggacg
     8401 agtgtcaaaa gcatggaaga gttcttcaaa gactggatga acttgaacca cacctactcg
     8461 cccatacaag tcttgcatca agcattgacc g
//
LOCUS       DACGLH010000103         8351 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.108, whole genome shotgun sequence.
ACCESSION   DACGLH010000103 DACGLH010000000
VERSION     DACGLH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.108"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..729
                     /locus_tag="IXT21_002612"
     CDS             <1..729
                     /locus_tag="IXT21_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4869976.1"
                     /translation="ETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQL
                     TKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKN
                     RASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELVEAFRDKDP
                     DLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNTHIKTMKRV
                     SYGFKSFENMRIRIFLINQLINVR"
     gene            complement(908..1243)
                     /locus_tag="IXT21_002613"
     CDS             complement(908..1243)
                     /locus_tag="IXT21_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4869977.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1227..1499)
                     /locus_tag="IXT21_002614"
     CDS             complement(1227..1499)
                     /locus_tag="IXT21_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ4869978.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1950..2753)
                     /locus_tag="IXT21_002615"
     CDS             complement(1950..2753)
                     /locus_tag="IXT21_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ4869979.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(2731..3855)
                     /locus_tag="IXT21_002616"
     CDS             complement(2731..3855)
                     /locus_tag="IXT21_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869980.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(3871..4686)
                     /locus_tag="IXT21_002617"
     CDS             complement(3871..4686)
                     /locus_tag="IXT21_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4869981.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(4676..5557)
                     /locus_tag="IXT21_002618"
     CDS             complement(4676..5557)
                     /locus_tag="IXT21_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4869982.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(5633..6922)
                     /locus_tag="IXT21_002619"
     CDS             complement(5633..6922)
                     /locus_tag="IXT21_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4869983.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7150..7794)
                     /locus_tag="IXT21_002620"
                     /pseudo
     CDS             complement(7150..7794)
                     /locus_tag="IXT21_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2594 a         1790 c         1312 g         2655 t
ORIGIN      
        1 gaaacaggaa acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat
       61 ttcttaggct gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct
      121 tacttccagc ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat
      181 attgtcaaac acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt
      241 cgtaaagcag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta
      301 aaaaatcgtg caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca
      361 aaatacccat ttctcactga agcaatgatg attgatcgat tgcttgagtt ttctgcgcct
      421 ctaaaggagg cgtatccctt ttttcatgaa ttagtcgaag cctttcgaga caaagaccct
      481 gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacgg ctttcgggag
      541 aagcttcaaa accttctgac ctatgaagaa ggtatcacca gcgcaatgat ctatccttat
      601 tccaatggaa aaattgaagc gaagaatacc cacataaaga caatgaaacg agtatcctac
      661 ggatttaaat cattcgagaa catgagaatt agaatctttt tgatcaatca attaatcaat
      721 gtaagataac aaaaaatctg aaccagaatg tggttcattc tgacccggat ttgattttgc
      781 acaactcatc agtccttatt gacaaagagc caaaaatctg agccagaatg aggttcattc
      841 tgacccagat ttgattttgt acaacccatc agtccttatt gacaaagagc ccttcttttt
      901 agtattatta taattcttcc atgcgttctt ccagtcgctg taaatcgctt ggacttaatt
      961 ccccaatttt tttccatttg acctttttgt tgtttttggg taattcgttt agagttccta
     1021 cgtcaatcca agattctttt tttagtcctg cctgtttcca atcccttatt tcaacgtatt
     1081 tcagctttat tttatcggat ttagtatgat attttgaagt aattgacaga aacgaaattg
     1141 tgtccagtcc attttttaga atatagacag gccggcgttt accgccctct gcagctgtga
     1201 aagcaacgta gacggttgct atatcattag tattcatctt cattgaacca ttcttcaatt
     1261 ttttcgtcag tgtctagttt ttggacaggc aatttttccg ctaatctttg aatattatgg
     1321 attcgtttac ttttttccga caattcaata gaaaatggga tttttccttg ctcaacaatt
     1381 tgcttataaa aaacggtgac ggctgttttg gggctcatgc ctagttcatc aataatgctt
     1441 tctgcttcat ttttcaattt ttcgtccata gaaatttgta acttagccat tttctccacc
     1501 tctttctgac aaacacatta tatcacggaa agagttcaaa tggaattaat acaactactt
     1561 ttgaactcct tgaaatgcag tgtatttatc taaatataag gaaaggatat agtgctaaat
     1621 ggccttgata gatagaaaag tttcaaaatc cgccacagcg ataattgaag cgaacactca
     1681 acgacacaca gaacaggaaa agactcctaa aagaaccaaa ttaactagga cgtgtctgaa
     1741 aactccacaa agatgtggta tgctctggta aaaaacgaat ggcacaacca cgaagaccga
     1801 tatggttctt ggaaaacttt ctccaccctt tttgtaattg gagggatagt ggtttattga
     1861 tagcgatttt ccaagttctt caagtggaac cagacattga gcaccacggc taaaaagtga
     1921 atgaagtctt tatttttaat caataatttt taatgattac cagtaattgg ttttttcatg
     1981 cgccctattt ctatttgatg tggagttaaa taatgctgat aagtaaaaat ttttccgtca
     2041 attaaactgc cactagtgaa tcctgttcta ttggtaaaaa gagaatagtc tgggtattca
     2101 tctacaccaa atgataaaga ttcagccata aactgttttt taccagcaaa ttcagcactg
     2161 ctaccttgat ggatatgtcc tacaaaaata cctaaaatat cactttcggc aatcaattca
     2221 gcaaaacctt ccggaacttc agttacaata gatggatctt cccacgacaa tggatgatga
     2281 accaataaaa tcgttccctt ttcagcaggt gtacccaacc actcttgtaa ttgaatcatc
     2341 tgtcctatcg atatttttcc ttcgtgctgg gtatcttgag cagaatccaa ggaaataatt
     2401 cgtaacccat tttgattcgt acaatggata tactctcccg tttcattgct ttcaacactc
     2461 attccttcaa agaaaacact gcgacgatca tgattcccac gacaaaaata gtaaggtgtg
     2521 gttgggaaat attgatccaa cattttccgt aagacttgat aatcagaaat ccccccttca
     2581 tgtactaaat cacctgttaa tagaataaaa tcgtacactg ttaacgagag ttcttgtaac
     2641 aatttttcca atgccaactg tggattttta attgttggaa atagttcagc agtaaacaag
     2701 ttagtttgat agttttctct aaaatggata tcagagagat gcaaaaagcg catggctatt
     2761 ccctctttct tcaataaaat aatcaggata tcctaaattt tcttgcgtct gataatcaaa
     2821 aaaatgcatt ctttcaggtt ctatgcggag attgactgca tcaccaatct gccaagtagg
     2881 tttttcatta aggacaatca tttctatacc atcgacttta acggtgtacg cataactttg
     2941 acctaatagc tcacgatact tcacttgacc gtgtaaatcg cttgtttctt ttgaaaaggt
     3001 tacgtgttct ggtcttatgc ctaaatacaa tgagccacta acctgaagaa gttgtttttg
     3061 ccaagctgga ttcaatgtta aaaattgact ggctaccttt aatgttcctt gatcataagt
     3121 aactggcata atattcatgc ctggcgaacc aataaaattt gcagtaaaag tatttttagg
     3181 tttcgtataa acatttttcg gcgtatcaat catctgaatt ctaccttcat tcaataaaac
     3241 aatccggtca gctaacgtca tcgcttcaat ttgatcatga gtgacgtaga caaaagtttg
     3301 tttataacgt tgatgaattt ttaccaattc ttttcgtgca tccaatcgta aacgcacatc
     3361 caaatttgat aacggttcat ctaataaaaa ataattactc cgtttaacca ccgcacgagc
     3421 caatgctaca cgctgccgtt gccctcctga caaatcttta ggaagtcgat cttggtatgg
     3481 cgttaaacct aacatcttca aagcttcttc caaacggtgc tgaatgtcag ctgtcttaat
     3541 tttattcgcc cttaatgcaa aaataatatt ctcctttaca gtcatgtgag gatataaagc
     3601 ataattttga aaaaccattg cgatctctcg ttctccacaa ggtagcttat ttgctagttg
     3661 tgtacctaaa aaaagctctc cactcgtaat tttttccaaa ccagcaatca ttctcagcaa
     3721 agtagatttt cctgaaccag atggtcccag taacacaatt cgttctccag cggccacagc
     3781 taaatcaatt tcttttaaaa tggtatgttc tccatatttt tttccgatat ttcttaattc
     3841 aagcgtttct cccatgattt gtcctccaat ttatttaata cctgcagaaa caaaagtccc
     3901 gataatatat ttttgaaaaa ttgcatacaa gactaacggt acaaacattg taattaaact
     3961 aatcgccatt gctacggtaa aatttgtccc accttcagaa ctaatatagg tctgcatcgc
     4021 caacggcaaa gtataattat ctactgtccg taaaattaat accggccaaa catactcatt
     4081 ccaagaggta ataaaaaacc aaataccggt agaagtaatc gcatgtctag taagaggaaa
     4141 aacaatttta ctcatgacgg ttgtttgttt gatgttatcc aatgccgcca catctagaag
     4201 gctcactgga atttcccgca ttgtttgcgt caataaaaaa atgcccatcg catcagcaaa
     4261 ttgtggtatt atcacacccc aagtagtatc aattaaatta agttttgcaa tcaacaaata
     4321 attaggaatc atcgcaacag taaaaggaat gaacatcgta ccaatgatga taaagtacca
     4381 gattttcttc cctttgattc ggcaataagt aaaactatat gccgcaaaaa aagctacgcc
     4441 taacttaaca accgttataa ttgtagcaat aaaaaaagta tttctcgtaa ttttgataat
     4501 tggaagattt tcgaacaaat agcaaaaatt ttccaaggta aacggctgtg gtatcaaact
     4561 agtaatcgtc tgataggcat ctttcaatgg cttaaaagaa ttggccataa taaacaataa
     4621 tggatagata ataatgatta tgatgaacat aaataagaga tgtggcaacc agcgtttaat
     4681 tttcatagta aacccccttt tccacaaatt taaatattac ccctaagaaa acagcaaata
     4741 ttaataaagt taaaactgaa attgctgaag attctccgac tttatacatt tcaaaagcct
     4801 tttgataagc ataatagatc acgtttgcac taccattatt cggtccacca cgagtgatga
     4861 tcttaatcgg agtaaacaca tactgcagac cttgaacaat ggtcatcacc aataaatata
     4921 gtcctgtcga actactcata ggcaacacaa ttgtaaaaaa aatgcgccag atgggtacac
     4981 gatccaactt agctgcttcg atcatctctt gatcaattcc cacaattgct gcaaaaatga
     5041 caatgaaatt ataaccaaaa actttccaag cagtaatacc tgcaatcaca gcaattaccc
     5101 aaccttgtaa attcatccac ataggcagtc ttaaaccaaa atttcccaac aagacagcaa
     5161 ttggtcccga taccggattt aatatccatg taaaaaccat ccccgccact accaaagaaa
     5221 tcacagctgg aataaaaaat aatggtttat acaaattctg ccctttttct ataactattc
     5281 ctaataaaaa ggatgtaata tatgggaaca cacagctcag caagacaaat agaacggtat
     5341 aggacaaagt attcttcaac actttatgaa agactggatc agtcagcacc tttacatagt
     5401 tggcaaagcc aacaaattct ttagtggggg ccaccatatt ccagtcaaaa aaactcaaat
     5461 agatcgtata aatcaatgga taaaaaacaa acacgcccaa caaaataatt gctggcgcca
     5521 gatacaaata acctacatag cgcttcattt ccgacagcct cctcatcctt atgtcaaaag
     5581 cagacgacct tgtttttcct ttcttacagg cgctgtcgtc tgcttgcttt gcttatttga
     5641 gtaactcatt aatttccttt tgagtagctt tcatcgtttc attgacggaa ttacctgata
     5701 aaattttatc ccgcatatcc aacaattttt gttcagcttc taatccagca tctccaggaa
     5761 atgaactcca actgaccatc ttatccatct gatcaattgc tggtatcatc atctgatttt
     5821 cagctaagaa agcttttaat ccattttggg cgtctgcaac atctttacgt ggtggtaaat
     5881 aaccagtacc ttctgtccat ttagctaaag attcaacact ataaagatat tttaagaaat
     5941 cccaagttgc ttcctttttc ttatccgttt gtgatgtaac agctaacatt gcgccaccag
     6001 caggtagttt aacttttttc ccttcccaac ttggtgactc aactgcttta gcagtaaaag
     6061 tactattttt ttgaatgttg tccctttgag caatagtggt atagaccatc gcgacgttac
     6121 catcaataaa gctttgcaca ccttgttccc acgtagtgtg taatgccgtc tggtctttta
     6181 ccatcctttg atataattca taagcagcga taccatcttt agaagcaaag gtagctttgc
     6241 catcttttat tacacttgcc ccattacttt ctaaaattgc ttgttgtgcc caattatcag
     6301 caggttcttg aatatacacg ccatactttc cagttttttc ttttattatc ttagaaaaag
     6361 tttgaacttc ttcccaagtt tttggaccat tttcatccaa gcccgctgct tttaatatat
     6421 cttgatttaa atataaaacc ggattactaa tggagtaagg aatacccact tgtttaccat
     6481 ctttttcaga cagtgccatg atattgtcta aaaaatgatc tttaaaataa tcagcattat
     6541 ccggatccac ttcttctact aaagattgag gattagtata tttgaaattt tctgcaaaat
     6601 aatctttaaa agcccatccc acttgtacta cgtccggtat ttctttggag gcaacagaag
     6661 cttgtaaatt ttgcatcaag cctttataca tatcaggatt atattttgct acaaccttca
     6721 ctttgtcttg agattgattg tatttatcta ctaattcatt cactgttttt ccacctaggg
     6781 tatccgcatt aacatgccaa tactctatcg ttacacgacc ctccgatttt tttgttgtct
     6841 tggtattccc actaccacaa gctgccaaag agatgacact taaaacagac acaaccccta
     6901 taacaagatt tttctttaac ataacattaa cttcctccta atttctttat ggccattttt
     6961 ctaatcaaga aatattatga actaccaatg taaatttcta gttaattttc tgtaaataat
     7021 tcgaaatttg tgcatttcgg ggtacagggt cactggacat atccatcaag ctaagaataa
     7081 agcccccata tggtcatgta tcccatctcc aaatctctta actcctctgt tttgattata
     7141 aaatagttta ttctgtgctc ctttcttcca taaataggcc catgcacaac gtgaggttat
     7201 attataaaca ccacagcgcg caaggtactc aatcgtttct agttcattga ctgtccatac
     7261 agaaaatgtc aatcctagct gctgccccac ttcacgggcc aacttagtta accatccttc
     7321 atgaatattg acgatttgaa cgctttgata ggctaatttc tgtaaggtat cgatcaaatc
     7381 tgcttcggta atatccccaa gttttttccc ttcgaactga ttttcaatat tataaaatat
     7441 atgctttccc caaatcgcag cccaagataa ttgtacatag ccactaagaa caatttgtgg
     7501 ccagatttga taggtttgtg ccagcgaaaa aacggccatt tccaatccct cttcttttaa
     7561 atcacagtta atggtcatgg ttgccttctc attggccact gcttgaaaca cctgctcaag
     7621 ggttaccgaa tctgggacaa cttcttacct gtcatgggct agtattagcg taccatctgt
     7681 ttgctgacgc acatctactt caaaacaaag ctggcgatcc aaacaaaagc gaagaaatgc
     7741 ccagctattc tcctccgtat tgtcactacc agagtgtgca gtgatcatgg tcattttgac
     7801 ccctccttta attccgtgat tttctcccac acgtccacta taccgatgaa gcgttaattt
     7861 cttgttaatt tcttgtaaat tccctgcgtt ttttcagtaa actagttgta aatcttgcat
     7921 gctccttcct acgaactcat gaatcgtcga ttttgccctt ctagctagtt tcaccaatta
     7981 agtagttcta cattccccgc ttttaaccta ctacaccttt ttttgttttc agctattcct
     8041 tcttcttgct gaaagtcctt gaggttcttt cttacaggat accatcaagg tgttcaatgg
     8101 cttattccca ttgaatgacc gagacttact atcagatttc tgctcgttga accaagtctt
     8161 tgatacagga tttcttccgt catcttctca caaataccac cttgcgtttc tctagcgatt
     8221 ggtcgatgtg caccctccca gtggactgtc actacccctt tttgctatgc ctagcccaca
     8281 aaaaaagctg tcctcacaga cagccagact gtagacaaaa gctgttccaa actcgtaact
     8341 cgagcttgga a
//
LOCUS       DACGLH010000104         8323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.109, whole genome shotgun sequence.
ACCESSION   DACGLH010000104 DACGLH010000000
VERSION     DACGLH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.109"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..278)
                     /locus_tag="IXT21_002621"
     CDS             complement(<1..278)
                     /locus_tag="IXT21_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869984.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFA"
     gene            complement(329..1957)
                     /locus_tag="IXT21_002622"
     CDS             complement(329..1957)
                     /locus_tag="IXT21_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869985.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            complement(1977..2180)
                     /locus_tag="IXT21_002623"
     CDS             complement(1977..2180)
                     /locus_tag="IXT21_002623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869986.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            complement(2181..2870)
                     /locus_tag="IXT21_002624"
     CDS             complement(2181..2870)
                     /locus_tag="IXT21_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAQ4869987.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(3125..3445)
                     /locus_tag="IXT21_002625"
     CDS             complement(3125..3445)
                     /locus_tag="IXT21_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869988.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3478..4596)
                     /locus_tag="IXT21_002626"
     CDS             complement(3478..4596)
                     /locus_tag="IXT21_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869989.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4625..5419)
                     /locus_tag="IXT21_002627"
     CDS             complement(4625..5419)
                     /locus_tag="IXT21_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869990.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(5429..6304)
                     /locus_tag="IXT21_002628"
     CDS             complement(5429..6304)
                     /locus_tag="IXT21_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869991.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(6521..6778)
                     /locus_tag="IXT21_002629"
     CDS             complement(6521..6778)
                     /locus_tag="IXT21_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869992.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(6781..7005)
                     /locus_tag="IXT21_002630"
     CDS             complement(6781..7005)
                     /locus_tag="IXT21_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869993.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(7178..8221)
                     /locus_tag="IXT21_002631"
     CDS             complement(7178..8221)
                     /locus_tag="IXT21_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ4869994.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2354 a         1703 c         1033 g         3233 t
ORIGIN      
        1 caagcaaaaa tttctaatat cacattaaat cttttcattt ttccaaaact actaagactt
       61 gatactaatt caatcaaaag cttatcgcta cttgctgatt catcatattt agttcctaga
      121 ttatctaaac ttagcatata agaaattttt tcctgatctg ttagttttga aaatgaactt
      181 tcttttaaac tttggattat acaatcagca ctaaatattt cgtttaataa agcagttttc
      241 ttttctaatt ccttagaaga taaagttagt tctctcatag tgacttacct actttccaaa
      301 ttttgtgtcc tatttatcac ttctctcttt atataagtga taaaagatcg taaatacgtt
      361 taattgttaa gttcatattc acatgattat ccaaataaaa tatcttatct ttattagaaa
      421 tttcactcat aacacgataa tatagaccat gattcaagta atctgtatat agaaaaatcg
      481 catcacacgt attcaaattt ttaagtatct ttccattatt ttcgcttaca tcaaacattt
      541 taatatttgg taatagttca gttaatttat tttgaaaatt tacatgtcct cccactaaaa
      601 gaatgttttt atgttttaga taagctatca tttcagacag tgaaggctct tcagttttac
      661 catttaatga ttgtctatgt tcttgctcct gcaattgctt taatacttta ttttcaaacc
      721 tcagctgttc aagttctttt ctgattcctt ttaattcttt aatctcttgc ttcaaaagtt
      781 cctcttcttt ttttgccgat ttgactaaag attctaaccg accgttttct ttactcgcaa
      841 tacttaattc ttttcttaat ttgattactt cagatttgtt ttcttttcta atacttgcaa
      901 tttgttttgc tatttcataa tcagaattca ggatcaattc ttccttagca tttttaaaca
      961 accgagttaa aacgaactga tataacaaac tataaaaaac tagcaaatag tcaatttgtc
     1021 ggtactcggt attctgatca attgcatata gagagattaa aacgaaatac acaatatcat
     1081 tcagctcgct acccttgatc tcaaaattat tgactaagta actatcaata cccatcaaat
     1141 ctgcaaaatc tccatacaaa gaaaaatatg attttgacaa tatatctata tctgaatcgt
     1201 caaacccatt tactattttc ccatcattgt tagtagttgc taacgaagca atagtgactt
     1261 ttgaattggg ttttacaaat cctataccta atagttcttt catcttttta atttcaagtt
     1321 tgttatcctt ttgttcggct ttatgctgtt tcatattgta aagcggataa aatgctgttc
     1381 cgtaagcacc ttttagtacg ttatctataa aataatcttc agaacctgtt ttgaatagaa
     1441 agttaatcgc agccaaatta gataacagct cttcagtgtt gtgcatcaat tttttatcat
     1501 ttaataaatt atccccaaga tatttcattg cataataagg gtttgtttcg ctacttattt
     1561 ttaatatttt ttgatagcta ttcctcacga tattaaccaa aatatctgtc tgattaattg
     1621 aattacagta caaagccact cctaatgcca tataaacatt caaatcatgt tttgccgtac
     1681 cgttgaataa aacgtcttca acttttgaat tatctcgtct atactttttg atcatatttt
     1741 tgatattttg atcattctct tggtatattt ttttcactcg ttccaatgcc ttgggtttgt
     1801 tggaaacagc aacagcgaag tctagttctt tgaaatcaat atttagtatc tgtacctcat
     1861 cacctagatt ataatctcta tttacgctcg gaaaactgct gaaatctaac tgttttttac
     1921 ctatttttgg cgaaaatatt ccttcgattt ctggcaaatg taatcacact ctctccttat
     1981 ttatctttag aaaatttgtc actattctcc ttatatttat caataaacct ttttaatcga
     2041 gcaatataag cgttaaccct tttaggttct tcttttcgcc attcatcgtt tacgtgacca
     2101 ttttcaaaga aagaagctaa aagagtttcc acttcattaa ccatatctgc gatcgtatat
     2161 ttttctgtac cctcactcaa ttattcatga actgttaagt gttcatagct aagttcattc
     2221 ataattttgt tatagcgttt tcttgctttt tcaaattctt tctgttcgtc aaattccact
     2281 tgattcttca tgttgttctt cctctctgtt tgatattttt tcacttcttc aagtttagca
     2341 tctagttccg ctttaacttc tgtcacttct ttaagcgctt gggataactt atgagacaat
     2401 ttcgcataag tttctctctt gtattcattt cctactccca actcttcgga gtagaactct
     2461 tctagtagtc ttgcaaaatt aacatttctc ctgccagcat agttttttag cttctcaaaa
     2521 tcagattgaa agacagcaac acttttgtaa tccttttcac cctcaaaatc aaaagcatag
     2581 tcatactttc ctgaaatcaa ttcatcaata actgtagcaa tatattgaga gatagaaata
     2641 ttatcttcac ttctatgtcg ctctataaga ttatatatat cagggctgac atgaaacgta
     2701 cttctctttt ttgtttgcct ttttctaact gttacgacca cttttggaac ttcactatct
     2761 ccattgatct ctttgtattt ttcaataaat aattttctag cttgttctcg actttttgcc
     2821 ggaatttcat ctacaatttt atcttctaaa taaatatagt gtgttctcat acattcgtcc
     2881 tctatcttct ttactctcaa tcttcatgat cttagttctt accttgttta ctcttaaaca
     2941 ttgcaaaaaa atctagtcaa aatcaaactt gaaatcatcg tctatcacaa cacccttaac
     3001 ttcatcttta gatttcccaa aggaaccctt attttcaaat cctttatttt tgaatgatga
     3061 tggttatgga aaacttcctt tgaaattctg acaagcataa ctagtcagaa ctgttttact
     3121 tagattaagc tttcaaaaga tactgatgaa gaactaagtt ctcagtacct tcccattctt
     3181 caataaaaaa tatttctcct tcttttaccc actctatagc taatttctta taatcaaata
     3241 aaccatatgt gtttaagcct ttgtctttca ttaaactcaa aaactgatcc ccttttaatc
     3301 tttctgtatc atccatcaga aattcaacta aatctttatc catgcgtgca actttttcgt
     3361 cttttagtgc cattgattta gcatagtcag ttgaatcttt tagaataata acacctacat
     3421 ttccttccac aattttcttc ttcatattta cctcctacca tcttttccaa agagtattta
     3481 gtcaaaatca aactcgaaat catcgtctat cacaacaccc ttaacttcat ctttagattt
     3541 cccaaaggag cccttatttt caaatccttt gtttttaaat tctaaagctt tttcgacttc
     3601 ttctttggat attgtatgaa cttttttctt aactactcca agtttataca gctcttcaaa
     3661 ctctggtact tcggatctga aatacaatcc atcagtacga atttttaata gcccatatgc
     3721 gctatgaacg tatttctgtt catatctaac catacgatct ctaaatacgc ctgctaccat
     3781 ggaggaatac gctcgattat acatactgta tttatcgaag gtgcttgttt ctccttctct
     3841 taatgatttt tgtccgatag caccaaatac tttcgttcta agatttttat aaagcttcaa
     3901 cgtatcagga tcagttttta atggctcgat tctttcaaaa tattctttga gtaatccttt
     3961 tttcgtcaaa aatgtgtggc tatctaggac ttccatctct ttaatatcat aataatcaag
     4021 gataagtgtt agctcaatct ccgataacca aataggctca ttagctgttt tgggattttc
     4081 gccataatcc gcaaacatcg actgtgattt tggattagca tatgttcctt ctttggctgt
     4141 tccttcaaaa ttaaccaaaa agaacatata cgttcttaaa ttttcatacg gtcttacttt
     4201 tggtctattt atcgctctca aaatagcttg atctgtatta tattttgctt tcaaataagt
     4261 gtttgacggc aaaggataat ttctcatgat cctcgaatag gcttcgttga aatcatattc
     4321 gatcatcttt gctccttcct caaacaaatc caagttatac atgctaccgg aggtactggc
     4381 tctataggct gaacttggaa caaagaaagg tgcatcttcc ggcttatcat agcccaaaaa
     4441 atgtattcct gtttgcttgt tctgacggta aatgtccatc aaaagatatt tgatcgtact
     4501 aaaagaatct aattttcctc ttgtcatcca ccctaatccg acaagtcttt taattttatt
     4561 ctgcatttca ggtgtgtata tgctattgtt tcccatttat ctgctcatct ccggcttttt
     4621 tattctagac cctatatttg ttcattacac cacttatctc gtctacaaag gctcccagat
     4681 cgtgtgtaaa ggcatcatag taatcttcaa tctcactcga ttcagttctc cggtttcttc
     4741 cgtttttatt tctgaataga tacatctgcg tttctcttaa atcccttgct tttttcgcaa
     4801 gtgctaataa atccgtaaaa ttatcaataa ccataatgtc attgatcata aaaccttctt
     4861 catgaccata cttttcagcc atctcgataa aatcattaac atcgaacaac tccaataatt
     4921 ctgctttata tttctctaac aatgtattta aatagcgatt tctcaattcc catgaacttt
     4981 caccagttcc gtattcaacc ttgatcccta aattcttagt gtagcctttt gctgtgtatc
     5041 gtccaatgaa gtctccctta ttccgattgc tatcttcttc tttgaaaaac tcacgccaaa
     5101 tccccttttt cttcttagga attagacctc tgctcttgaa atcatcatat ttttttgaag
     5161 cttctcttcg tgtatctaag tacctttgct tcagctctgt agctgattca ttcgttccat
     5221 attcaacagt gatccttaag cgcctgctat aaccttttgc tgtatatcgt ccaacaaagt
     5281 caccgccact tcgatcacta ctgacttcct taacaaattc tctccatata ccttttttgt
     5341 cctttggtat taatcctcgt cttttcatat cttgatattt ttcagaagct tcttttagag
     5401 tattcttttt tgctaccata ttttccactc actctttgtt atctagtatt ttaataactg
     5461 gtttcatttt tgtaatatct tctttttcag ctatatacaa ctccgttacg gtaacgatct
     5521 tagtatgatt tagattaatt actttcactt gatcgccaat tttgattttt cctcgcaacc
     5581 gtttgggaat aaaccagttg tataatcgtg atttattatt atctacataa gcttttattg
     5641 cattttcagg aaacaggtta tttagtcttt caattttttt acttttaaaa aattcattcg
     5701 caaacttttc atcaacaact aaattaaata aagtttcaga atttacagct tccatatcta
     5761 ttcctttgta gataggagaa agcaattttt tatattttgc cagtaatgac tttccagaat
     5821 tactaatctt aatttggtta cttaaatctt cttgataaag aaccccatat cgaagtaacg
     5881 ccttaatata atgttttaat atgccgttga taccagaata aatatccatg ccgttctcaa
     5941 tatagtagaa agtgtcgtct ctccaaaaca atttgaagct attattcaaa taaagtaaca
     6001 agatcaatat atgtttgtca gaaattctga caaatctatt actttttaga tactcaccaa
     6061 tggcttcact taactctttg aagccattgt attttcccga taaaaatgtc gtaacattat
     6121 cttctctctt tttcctatgg tgtgaactta ccctgaaata atagagtcta ttctcattta
     6181 atattgaaaa ataaatactt tcagttgttt gtgagcattg tatatagttg aaatgatatc
     6241 catctggcat gactttttta actgtatttt gtactagctc tgaccacctt ttgttagtag
     6301 tcatatattt ttttccatta aaccaaacat aaatatcttt tttaaaaatt ttgttccccc
     6361 cctattgcgt tttctttgac attaaagccc tttgttttca tcacattcgt atagccctag
     6421 ccactcataa cctgatgctt ttgcgaagtt tatttaaaag tgttttcctt tgtaatctac
     6481 ctttcccctc gtcctggtac ttttcttggc gactattaaa ctattcaact tttttagaat
     6541 cattgtcagc ttgaaatcct tcacgtttca ttatgtcttc tgcttttcca atgctgattc
     6601 ctttccaatc acgccatcct acgcttccct tattttgacg ataggcttta atagctgatt
     6661 cggatatttg ccatcttgta gccaactctt tggctgtgta cttatttgaa aaaagtagct
     6721 tctctatcag tgaaatatca atcattttat tctcactcct gaaatcttca ttaatcatct
     6781 ctagctagct atatcttcaa tcaattctaa tatgtcggat aaaccatcaa cagcttcttc
     6841 aagttgatca acggattctt ctgctttttc atatctttct gatccctgta gatttttagg
     6901 tatattttct ttgtactctt cttcttgaat tacttctaac tcatccttta gctcggaaag
     6961 tcgctcgctt atttcttcta acctttttct tcttctttgg ttcatcaaaa tcacctcttc
     7021 gcataatctc tacgatttta atataccact tttgtgtact acattcaata ttttttgtta
     7081 tagattcgta ttacaaaaat ttcctctaaa aaaataagag cctatttacc atttcagtaa
     7141 ttaagctctt atttcattta gttgataatt ctatcaatta acgtacagca accgccattc
     7201 cagtcacatt aaatgctctc tcatacgtgc ttgcattgat ccattttgcg ccaaatacag
     7261 ggtcaacaac atggtatgca ccatttctga agccatccac gagaactaca tgtccattcc
     7321 atactgcacg accccaaggc atttgtttga actctggcgt agcatagtta taagtaatcc
     7381 aagttacaac cggattacca ttgcgaattt cattcttgat tccattcgtt cctgaaccag
     7441 tgatattgac ggcattaccg ccaacgtttt ttgcccattt aactactggg tcagccatca
     7501 tgccttgata cgttccatta acgttatgac gccactctcc tgagtaacca tgataaggat
     7561 tattgtcatt agcttttggc atatagttta caaaactggt taagctttga tttgtggcta
     7621 cacctttgta gtgtaaacct tctaataaag aagctccttc gcataacatt gtatttcctt
     7681 gccatacaac aggaacgttc aacacttttt cagtgcttgg agcaggtgta ggatttggta
     7741 cgctgtttgg cattgcatac cagcctgtac cttcaatctt ccaaccttgt ttactaatta
     7801 cgttcttttc attttgatct aacgtgtagt gatgccaatt cacgtatttg ttatataaac
     7861 gtgttacagg aactctattt gctccgttgt aggaatagaa cgcaacacct tcgtacgtcc
     7921 atcctgtgtg tgaaatctta tctctttctc cggcatcaac tgtgtaaaaa tgttcaccgt
     7981 tataccgatt atacaaacga taaacaggag taccacttgt tggagcttgc catgcgacac
     8041 cttcgtaatg ccatccataa cgtttcagat tgtctctttc tccaacgctt ctagtgtata
     8101 gatgctctcc gctattggga ttatacatac gatacatgga aatagagctt tctgctgaaa
     8161 ctgaaggtga taataatact agccccattg tgaatagaat tcctactaaa aattttttca
     8221 tttgatttta ccccttttca ttttttattg tcattattgc atgaaaatta ttcgtaagat
     8281 tgccaattac ataaacgatt gttttaagat cataatcgtt gtt
//
LOCUS       DACGLH010000105         8290 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.110, whole genome shotgun sequence.
ACCESSION   DACGLH010000105 DACGLH010000000
VERSION     DACGLH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.110"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(45..1511)
                     /locus_tag="IXT21_002632"
     CDS             complement(45..1511)
                     /locus_tag="IXT21_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ4869995.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IXT21_002633"
     CDS             1898..2494
                     /locus_tag="IXT21_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ4869996.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IXT21_002634"
     CDS             complement(2552..3034)
                     /locus_tag="IXT21_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869997.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IXT21_002635"
     CDS             complement(3140..3337)
                     /locus_tag="IXT21_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4869998.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IXT21_002636"
     CDS             3632..4390
                     /locus_tag="IXT21_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ4869999.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IXT21_002637"
     CDS             4450..4878
                     /locus_tag="IXT21_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870000.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IXT21_002638"
     tRNA            4924..4996
                     /locus_tag="IXT21_002638"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IXT21_002639"
     CDS             complement(5146..5517)
                     /locus_tag="IXT21_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4870001.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IXT21_002640"
     CDS             5732..5917
                     /locus_tag="IXT21_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870002.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IXT21_002641"
     CDS             complement(6000..6188)
                     /locus_tag="IXT21_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870003.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IXT21_002642"
     CDS             complement(6212..6475)
                     /locus_tag="IXT21_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870004.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IXT21_002643"
     CDS             6633..6890
                     /locus_tag="IXT21_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ4870005.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IXT21_002644"
     CDS             complement(6961..7152)
                     /locus_tag="IXT21_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870006.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IXT21_002645"
     CDS             complement(7293..7613)
                     /locus_tag="IXT21_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870007.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IXT21_002646"
     CDS             complement(7636..8070)
                     /locus_tag="IXT21_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4870008.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2628 a         1635 c         1314 g         2713 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg
//
LOCUS       DACGLH010000106         8251 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.111, whole genome shotgun sequence.
ACCESSION   DACGLH010000106 DACGLH010000000
VERSION     DACGLH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.111"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..197
                     /locus_tag="IXT21_002647"
     CDS             <1..197
                     /locus_tag="IXT21_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870009.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IXT21_002648"
     CDS             314..811
                     /locus_tag="IXT21_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4870010.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IXT21_002649"
     CDS             898..1290
                     /locus_tag="IXT21_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4870011.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="IXT21_002650"
     CDS             1274..3721
                     /locus_tag="IXT21_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4870012.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="IXT21_002651"
     CDS             3724..5907
                     /locus_tag="IXT21_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4870013.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="IXT21_002652"
     CDS             5904..6905
                     /locus_tag="IXT21_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4870014.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="IXT21_002653"
     CDS             6920..7834
                     /locus_tag="IXT21_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4870015.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="IXT21_002654"
     CDS             8079..8195
                     /locus_tag="IXT21_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ4870016.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DACGLH010000107         8129 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.112, whole genome shotgun sequence.
ACCESSION   DACGLH010000107 DACGLH010000000
VERSION     DACGLH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.112"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            144..1340
                     /locus_tag="IXT21_002655"
     CDS             144..1340
                     /locus_tag="IXT21_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4870017.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(1402..1587)
                     /locus_tag="IXT21_002656"
     CDS             complement(1402..1587)
                     /locus_tag="IXT21_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ4870018.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            1818..5132
                     /gene="dnaE"
                     /locus_tag="IXT21_002657"
     CDS             1818..5132
                     /gene="dnaE"
                     /locus_tag="IXT21_002657"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ4870019.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            5299..6261
                     /gene="pfkA"
                     /locus_tag="IXT21_002658"
     CDS             5299..6261
                     /gene="pfkA"
                     /locus_tag="IXT21_002658"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ4870020.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            6335..8119
                     /gene="pyk"
                     /locus_tag="IXT21_002659"
     CDS             6335..8119
                     /gene="pyk"
                     /locus_tag="IXT21_002659"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ4870021.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
BASE COUNT         2621 a         1448 c         1724 g         2336 t
ORIGIN      
        1 ttgttctgat ctctttttgt tttacataaa aagaacgtta catataaaat gacctttgcg
       61 ggatttttct ctttttactg gcgagatcgt tgcttgttta ttcgaaaata ggtatgataa
      121 aagagacgaa agagggatga tagatggaga ttaattggag aaaaaattta gtgattgcgt
      181 ggattggctg ttttttcact ggtgcaagta ttagtttagt tatgccattc ataccagttt
      241 atgttgagca actaggaaca ccaaaagatc aaatcgaatt gttttcggga ctagcaatct
      301 ctgtgactgc ttttgcatct gccgtggttg caccaatctg gggaaatcta gctgatcgaa
      361 aaggccgaaa actcatgatg atccgtgcag cagcagggat gacattaaca atgggatctt
      421 tggcttttgt accaaacgcg tattggcttt tgatcatgcg gttttggaat gggattttgt
      481 ctggatatat tccaaatgcc acagcgatga ttgcttcaca ggcacctaga gaaaagagcg
      541 gatgggcgtt aggaacattg tctaccggag cgattgctgg aaatctgatc ggaccttcaa
      601 tgggcggtgc actggctcag tggtttggta tggaaaatgt attcttgatc acaggggctt
      661 tgttgatgat cacaacggta ttgaccattt tcttagtcaa agaagatttc catccaatcg
      721 agaaaaaaga tctgataagc accaaagaaa ttttttcaaa aatggatcat ctctctattt
      781 tgatcggtct cttcattacg accttgattt tacagatagg aatcactacg atcagcccta
      841 ttttgacctt atatattcgt gaattgagcg gaagtacaga aaatatcctt tttgtcagcg
      901 gcttgattgt ttcgatagct ggtgtttccg ctgtcttttc ttcaccgaaa ctaggaaaac
      961 taggggataa gattgggaat caaaaagttc taattggagg actcatttta tcattttgct
     1021 gttatttacc tatggcattc gtcactacac cgctccaatt aggtatactt cgctttattt
     1081 taggcttttc aactggcgct ttgatgcctt ctgtcaatac actgatcagt aagattacgc
     1141 ctcaagaagg agtcagccga atctatagct acaatcagat gtttagcaac tttggacaag
     1201 ttttaggtcc catgctagga tcgacagtcg cgcatgctta taattacagt gctgttttta
     1261 ttgtgacaag tttgtttgtt ctgtcaaata ttcttctttc tttattcaac tttcgtaaag
     1321 ttcttcgcag cagattataa tggtggtgag gagtagaaaa taaaaaagag cttgagaaat
     1381 ctcaagctct tggctgtttt tttacgaatg ggcagaggtt tcttttcttg gcgcaactgc
     1441 accaatcaaa aggataatga cacccgcaat gaatgaaatg attgttgtca aagtaaaatc
     1501 atatgttccg cttgttaatg cagcaccaat ataaccgact acttgaccta gaacgacagc
     1561 ccagaataat gtaacgatat atcgcataaa gaacacctct tttctatctt aattttacca
     1621 gattttcttt gaatgtaaag aaacgagacc aaatctcaat aaaatttgag ttcttgcgaa
     1681 agaactcatt cctacagatt acaagcaagc aaatgaagtg gaataatcaa cttcagcgcc
     1741 tgctatctta atgaaagtaa aaggatcaaa ccttttaacg ctagtgaatt ttaacgaaaa
     1801 aaaaggcgga atgcaagatg tttttcccac aactttatac aattacttct tattctcttt
     1861 tacaaagtac gatacgtatt cgagaatatg tacaagaagc aaaaaaacaa ggctacactt
     1921 cattagccat aacggataaa gatgtcttat acggtgcacc tgaattctat cgtatttgtg
     1981 tagaagaagg agtacagcca atcattggat tacatcttag ttatacttat gaagaaaaaa
     2041 attatggctt gcttgcgtac gcaaaagatg aaataggtta tcagcagctg atgaaacttt
     2101 ccagccgaaa aatgatccaa gaaaaattgg aacttgcaga aatgacagaa accgaccatc
     2161 tagcgtttgt tgttccggca aatcattctg tttctgaggc tttcaataaa gaggataact
     2221 ttttcggtca ttggcagttt ttacttgaaa atgtttttac tagaaatttt tatatcggct
     2281 tgacagaaaa cacgcctgat caattgcctg aatggtatca gtatgtgata aaagaaaatt
     2341 attcgctttg tgcattacat cccatcgcta gtttacaaca agaagaactt tttgcacttg
     2401 aagtgatgaa acatatcaaa gagggaagcc aaataccttt agaaaattta tctgcatctt
     2461 taccactaac agcaaatgtc tatcttcttt cagaacaaga aatgatcaaa cgattcgaaa
     2521 aagcgggtct ttcccaagct gtaataggcg cccagcgttt agctgaatcg atttcatttg
     2581 aattccagtt ccatcagaaa ttacttccgc attatccagt tcctaatgga aaacaagctg
     2641 gagaatattt aagagagtta tgctattcgc aagtggaacg ccgaatcact gactatacaa
     2701 aggaatacca agaacgtttg gactacgagt tatctgtcat ccatcaaatg ggattcgatg
     2761 attatttctt gatcgtatgg gatgtaatgg cttttgctca tagaaacaaa atcgttaccg
     2821 gtgcaggtcg tggttctgct gcgggttcat tagtagccta tgtgttagaa atcacagatg
     2881 tggatccatt agaatatgat ttgctgttcg aacgcttttt aaaccccgaa cgctttacga
     2941 tgccagatat cgatttggat attcctgaca atcgacgtga agaagtattg aattatgttc
     3001 gagaaaagta tggacaatat catatggcac aaatcgcaac atttggcaca atggcagcaa
     3061 aaatggttct tagagatgca gctagagtgt ttggtttatc gcaaagtgaa gcaaaccgct
     3121 ggtcaaaagc aatacccaat caattgaaaa tcacattgac ccaagcttat aaaacttcgc
     3181 atcaattagt agaattagtt caggcaagcg aaaaaaacca acttttattc aaaactgcag
     3241 tgattttaga aggattgcca agacatgttt cgactcacgc agcaggtgta gttatcagcg
     3301 ataagaatct acttgaacta gttccgctcc aacgcggttc tgatgaagct tttctgaccc
     3361 agtatacgat gaatgaggta gaagcaatcg gtcttttgaa aatggacttt ttaggactac
     3421 gcaacttatc gattattgat tttactttga aagctgttaa gcgagtagaa gggtatgaga
     3481 tccgattaaa agagatcccg ctaaatgatg caaaaacgct gctcttgttt caacgaggcg
     3541 aaacctcagg agtatttcaa tttgaatctg ctggtatccg gaacgtttta cgaagattag
     3601 atccagaaaa catcgaagat attgccgcag tcaatgcatt gtatcgacca ggccctatgc
     3661 aaaatataga taccttcatt gccaggaaga aaggaaaaga agccattcgc tacccagatg
     3721 attcactgat cccgattttg aaaaacacat atggagtgat cgtctatcag gaacaaatca
     3781 tgcagatcgc ctcgactatg gctggattta cattagggca gtcagatatt ctgcgacggg
     3841 cagtgagtaa aaagaaaaaa gacgtattag atgaagaaaa gtcgcatttt gtatcaggag
     3901 cattacaaca gggatattcg gaaaaaacag cagcgaccgt atatgaatat atcgaaaaat
     3961 ttgcaaacta tggattcaat cggtcacatg catttgctta ttcattcgtg ggattccaaa
     4021 tggcgtatct aaaagcccat catccaggtg ctttctttac cagcctgatg aattctgttc
     4081 ggcataacac atcaaaattg aaagaatata ttgcagaagc acgtaaaaat aaactcaaac
     4141 tttcctcgcc gtccattaat caaagtaatt ccagttttga gttggtcaat aaggaagaaa
     4201 tcagattcgg actgacagcg ataaaaggta ttcggcgtga ttttgtagaa gatatattaa
     4261 acgaaagaaa acaaaatggc ccgttccagt cagtggatca attcctcata cggatcgata
     4321 agcgttggct gaaattagag ttactgcaat cactgacagc agtaggagtt tttgatgaat
     4381 tatctcctaa caggaaacaa ttgatgcttg atctcgaagg aaaaatccaa aatatcgttt
     4441 acagtggtgg aagtcttgac ttgctgggaa tcatggcatt aaaagaagaa gaagtcgctg
     4501 actatacact agaagaacgc ttgaagctgg aagaggagta cttaggtatt tatttgtcag
     4561 gtcatcctgc agaagggttt gatcgtcttc gtttagctaa acaagttcag ctgattacag
     4621 atatcgtccc taatcaacca gtcaatttat tagtgatgat caaggaagtt agagaaattc
     4681 ggacaaaaaa aggagaactg atggcctttg ttgaaggaac cgacagtagt ggagaaattt
     4741 ctgtcacagt ttttccaact ctttatcgta aatatcgtaa tttgtttgcc gttgatcgag
     4801 tgatctttgt tcaagggcgg agcgaaatca gcaaatataa tcaggaactg caactaattg
     4861 gagaaagctt tgctgatcct aaccagttag aagcccagta tccttcacaa acttgctatt
     4921 tgcgtattac agaagaacgg gaagatcccg agataatgaa acaattacaa gtgatttttc
     4981 agaaacaccc aggatatata ccagtagtta tgtatcacga aaaaacgcaa cgaaaagtag
     5041 ttctgtcaga tgcttattgg gtggatgaca gcaagagtat aaagcggcaa cttatttatc
     5101 tgcttggcga agataatgtg gttttcaaat aacttacggt aaaaaatact acttgctttc
     5161 atttttttca tttttttatc cgtaaatcaa gacaagtgag aaaaataatg ttagaatgtt
     5221 agtcgtggtt aacgcaaaaa tggttgtaaa cctaagtcat acttaggata aagtatttta
     5281 atgaggtgaa tgtttattat gaaacgcatc ggaattttga ccagtggcgg agacgcacct
     5341 ggaatgaatg cagctgttcg tgccgttgtt cgtaaaggta tttacgaagg actggaagta
     5401 tacgggatca attatggttt tgccggttta gttgctggag atattcgtcg tcttgatgtg
     5461 gcagatgtag gtgacaagat ccaacgtggt ggtacattct tgtattctgc acgttatcca
     5521 gaatttgcaa ctgaagaagg tcaattaaaa ggaatcgaac aattgaaaaa attcggaatc
     5581 gaaggtctag tggttatcgg aggagacggc tcttatcatg gcgcaatggc tttgacaaaa
     5641 cgcggtttcc cagcagtagg tattcctgga acaatcgata atgatattcc tgggactgat
     5701 ttcacaatcg gttttgatac agcaatcaat acagtactag aatcaattga ccgtattcgt
     5761 gacacagcga cttctcatgt tcgtacattc gttatcgaag taatgggacg caatgcagga
     5821 gacatcgctt tatggtcagg tgtggctggc ggagcggatg aaatcatcat cccagaacat
     5881 gattttgata tggcttcagt tgctaaaaaa atccaagaag gccgcgatcg cgggaagaaa
     5941 cattgcttga tcatcttggc tgaaggtgta atgggcggaa atgaattcgc tgagaaactt
     6001 tcagaatttg gtgactatca tacacgtgta tctatcttgg gacacgttgt ccgtggtgga
     6061 gcaccaagcg cacgcgaccg cgtgttggca agtaaattcg gtgcttacgc tgtcgaattg
     6121 cttcaagaag gaaaaggtgg attatgtgtc ggtatccatg acaacgaaat cgttgcgtct
     6181 gacatcattg atacacttga aaacaacaaa cataaaccag acttatcact atatgatcta
     6241 aataattcac tttcatttta agagtgaaag aaaccaagct atttaagctg tgtacagctt
     6301 taaataaaca aaattgaatg ggagagtttt ttaaatgaaa aaaaccaaaa tcgttagtac
     6361 attaggacca gcaagtaact cagtagaaat tatttctcaa ttgattgaat ctggggcaaa
     6421 tgtcttccgc tttaattttt ctcatggtga ccatgaagaa caattatcac gtatgactat
     6481 ggtccgtgaa gcagttaaaa agacaggaaa agatgtcggt attcttttag atacaaaagg
     6541 agctgaaatc cgtacaactg ttcaaggcac aacagaagct gattttggtc gtgcaggata
     6601 catcaaattt gaagtaggag atcaaacacg catctctatg gatcctgaac atgtaggttc
     6661 aaaagaaaaa atcgcggtta cttacccagg attatttgac gatgttcacg ttggcggaca
     6721 cattttattt gatgatggtt tgatcgatat gcaaatcatt gaaaaagacg aagcaaaccg
     6781 tgaattagtc gtagaagtta aaaatgcagg tatgctagga tcacgtaaag gggtaaatgc
     6841 accaggcgtt tctattagtt tgccaggtat tactccaaaa gatgctgacg atattcgttt
     6901 tggtttagac aacgatatcg actttatcgc tgcaagtttt gtacgtaaag cacaagacgt
     6961 tctagatatc cgcgaaatct tagaagaaaa agacatgact catgttcaaa tcttccctaa
     7021 aatcgaatct caagaaggta tcgacaacat tgacgaaatc atcaaagttt ctgacggtgt
     7081 tatgattgcc cgcggagata tgggtgttga aatcccagct gaactagtac caatggttca
     7141 aaaacgcatt atcaaaaaat gtaatgctgc tggtatccca gttatcactg ctacacaaat
     7201 gttagaatca atgcaagaaa acccacgtcc aacacgtgcg gaagcttctg acgttgctaa
     7261 cgctgtattt gatggcacag atgcaacaat gctatctggt gaatctgcaa acggggacta
     7321 cccagttgaa gctgtttcaa caatggcacg tatcgaccaa gaagctgaaa atgcattatc
     7381 agaattaggt acattccaat tgaacgaatt cgataaaaca gatgtaactg aaacaatcgg
     7441 tttatctgta gctcgtgctg caaaaaatct aggcgtgaaa acaatcgttg ctgcaactga
     7501 atcaggatac actgcaaaaa tgatttcaaa atatcgtcca gatgcagata tcttggctgt
     7561 tacttttgac gaacgcacaa aacgtggttt gatgttaaac tggggcgttt acccaacagt
     7621 tgctgaaaaa ccatcaacaa ctgatgaaat gtttgaacta gcagctaaaa aagctgttga
     7681 attaggtttc gcatcagaag gcgatttaat cttgatcact gctggtgtac cagttggtga
     7741 acgcggaaca actaacgtga tgaaaattca aatgattggt tctaaattat tagaagctca
     7801 aggcgttgga caaaaatctg tcgttgcaaa tgcagttatt gcttcatctg ctgaagaagc
     7861 tgtaaaaaaa gcaaaagacg gaatggttct agttgtacca tcaactgaca aagaatatat
     7921 gcctgcaatc gaaaaagcag ctgctgttat cgttgaagaa ggcggattga cttctcacgc
     7981 tgcagttgta ggaattgcac aagatattcc tgtaatcgtt ggtgctaaag gcgcaacaac
     8041 aacaattaca gaaggtgaat tgattaccgt tgattcacgt cgcggcatcg tttaccgtgg
     8101 cgaaacaaca gctatctaat ttagcttaa
//
LOCUS       DACGLH010000108         8067 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.113, whole genome shotgun sequence.
ACCESSION   DACGLH010000108 DACGLH010000000
VERSION     DACGLH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.113"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..883
                     /locus_tag="IXT21_002660"
     CDS             <1..883
                     /locus_tag="IXT21_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ4870022.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IXT21_002661"
     CDS             931..3060
                     /locus_tag="IXT21_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ4870023.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IXT21_002662"
     CDS             3035..3724
                     /locus_tag="IXT21_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870024.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4818)
                     /locus_tag="IXT21_002663"
     CDS             complement(4132..4818)
                     /locus_tag="IXT21_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4870025.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4954..5148)
                     /locus_tag="IXT21_002664"
     CDS             complement(4954..5148)
                     /locus_tag="IXT21_002664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870026.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="IXT21_002665"
     CDS             complement(5198..5638)
                     /locus_tag="IXT21_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ4870027.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="IXT21_002666"
     CDS             complement(5642..6487)
                     /locus_tag="IXT21_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ4870028.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="IXT21_002667"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="IXT21_002667"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ4870029.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1713 g         2358 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccgggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaaa
//
LOCUS       DACGLH010000109         7866 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.115, whole genome shotgun sequence.
ACCESSION   DACGLH010000109 DACGLH010000000
VERSION     DACGLH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.115"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(140..1093)
                     /gene="menA"
                     /locus_tag="IXT21_002668"
     CDS             complement(140..1093)
                     /gene="menA"
                     /locus_tag="IXT21_002668"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ4870030.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1106..2209)
                     /locus_tag="IXT21_002669"
     CDS             complement(1106..2209)
                     /locus_tag="IXT21_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ4870031.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2299..3234)
                     /locus_tag="IXT21_002670"
     CDS             complement(2299..3234)
                     /locus_tag="IXT21_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ4870032.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3536..5470
                     /locus_tag="IXT21_002671"
     CDS             3536..5470
                     /locus_tag="IXT21_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4870033.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5927..6343
                     /locus_tag="IXT21_002672"
     CDS             5927..6343
                     /locus_tag="IXT21_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ4870034.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6357..6923
                     /locus_tag="IXT21_002673"
     CDS             6357..6923
                     /locus_tag="IXT21_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ4870035.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6946..>7866
                     /locus_tag="IXT21_002674"
     CDS             6946..>7866
                     /locus_tag="IXT21_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ4870036.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPA"
BASE COUNT         2340 a         1536 c         1590 g         2400 t
ORIGIN      
        1 caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
       61 aaaaatataa ggagctattt ttatttggct gttcttatta cttgatgcag ggcagttttt
      121 tcacaacctc ttcatgctgt taaatggctt gcataatgat tgttagtaat agccccaata
      181 cataactggc attgaataag accatatttt tgatggaata gctgaaactg ataggctggg
      241 gtaacgtttg cttgaattga tccaaatgct tcttcacaat tggcaaggtc aagaaggtga
      301 ccaagatcgg ccattcatag ataccgctca acaaccccat taaaatcacg aaatagcaag
      361 catacatcaa tagctggaat aagactgttc ccgcaggacg tccgatataa aaaactagtg
      421 tatagcgatg attttcaata tcacggtcaa gatcccgcaa attattagcc aacatgatat
      481 tggcaatcgt aaacaccaag ggtaaggaag ctaatacgat tgctgctata gcccaaagat
      541 ttcctaccaa atcaaattgt ccagatgaaa agttgactgt cagatcatag acatctgaac
      601 cgaatgtatt gacataaacg accatggcaa aaattccaag gcccatagtc accccactga
      661 aaatttcccc taaaggcatt cgagataaag gaatcggtcc gaatgtatag aatactccaa
      721 taaaacaaca gataaatccc atcaaaagta aaagccaacc cgtattgatc gttagaataa
      781 cgccgataac aaaaacaaat gcaatcatag ctaaaatcat gtttcgaatc aatacaggag
      841 atagatttct gcggccaatg acattttctt catacttata tacttcagaa tgagcctttt
      901 cataatccat gtaattattg atagcggtag tcgctaaatc aaaaactagc attccaataa
      961 aaaataaaaa agtgtttagc caatgtatct catgaaaaaa agcgatagag aataataccc
     1021 ctaccgcaaa aggaaataca ctagccacct ttgttcgtat ttcaactact tctaaaaaga
     1081 tttttagtgt catggttcgt actccttatt ttagttcaga acggtcaccc atcgtgtaat
     1141 gggagtcctc cgataatttg aaggtgtctt tgattggatc ggtcacatat actttatcgt
     1201 cttttgtcac gaagatcgct tccgtcccgt tattcaattc gctttcgacg tattccagtc
     1261 cttttttcac acccattgaa aagactgctg tagacaaccc gtcaccatca attgatttat
     1321 ctgttatgat cgtgacactt gcgatatcat tgtcgaacgg ataacccgtt tttggatcaa
     1381 acaaatgatg gtattttttg ccatctactt ctaaataacg ctcgtaaatt ccagaggtaa
     1441 ctaatgtttt gttccgttct ttaatagaac ctaaaacaga tccgcgagcc atgttcggat
     1501 cttggatgcc gactgtccag tcttggtttt cgcctcttgg gctgtggcct aagacataga
     1561 cattaccgcc caagtcaacg atggcagtgg tcactccttg ttttttcaag actttcacta
     1621 cttcgtccgt aatgtaacct ttggcgattg cacctaaatc gatgatcatt cctttttctt
     1681 ttagataaac agttttttct ttgtcattta gttcgatctt atgataatcc actaatttca
     1741 atgcctgatc gatttcttct tgggaaggtt ttctggcgtc atcaaaaccg attcgccata
     1801 attgtgtgat tgcaccaatc gccatatcga atccgccttg agaatcttgg ctatattcgt
     1861 aggcccgctt gactaacgtg tagacgtcat cagaaacttt tactggtttg atcccagctt
     1921 gctcattcac ttcatcgatc tcagaacctt tttgattgat cgtgatttta tcaccaagtt
     1981 cttttactcg atcgaaggca ggtttcaatg ctgattcttt gccttcatca tacacacgga
     2041 tacgaacgta agtccctaac aagaactgtt catcagaata tggatctttc aatatcttgg
     2101 tagctgctga actttcagtg gttgttttat ttgctgagtt gctgcagcca gttagcacca
     2161 taaaagagat gcccagtgtg attaaggaaa atagtagttt tttccgcatc tttttcctct
     2221 cttcattttg aaaggtgaga cgactgccta atctggctat cgtcccaccc tccaactttt
     2281 attagtatga ttatgtgatt atttttctgt tacgatatta tctacttcga ttgtttgagt
     2341 gtcgcctttt tcagctgcat tcactaattg ttgtgcgtac atgatgaatg aatgtgagct
     2401 gtgtgtagca cctgtaacaa cttctacatt agcaggtgag ccatcttctt cgcccattgc
     2461 tttgacgaat tcgtcgttca gagtagggat gtaagttttt ggttctgtac cgctttttgc
     2521 tttcatattt tcattatatt ctgtatcatc ttgttttgat ttaccgtctt tgtcgatata
     2581 atcaaagttt gattcagtta ctttgccgcc agcaactgtc atttcgaatt gcacacggta
     2641 gccgttagag tagttctttt cttcaagctt gtaagtgccg tctttcaaat cagcaccatt
     2701 gtcgatttcg attgtatctg tgttaccagc ttgagcagct tggatcaatt gttgtgcgta
     2761 gttttggaag ctttcagatg agtgagtagc tccagttacg acttccacac cgcttgcgct
     2821 ttgtgctttt aagaattggt cattcaactc aggaatgtat ttttctgggt ttgtgccaga
     2881 ttttgcttcc atgtttttgt tgtattctgc atcttttgtt tttgattcgc cgttttcatt
     2941 gacgttatca tattttgatt cagtgatttt gccgtctttt acaaccattt caaagactgc
     3001 acggtaacca tttgaataat ttttttcttc taatttatat gtgccgtctt gcaaatctcc
     3061 gccagctaca acttttgcag atgattctgt tgtgcttttt gcagtcgaag attgtgctgt
     3121 agatgattca gaagaagagt ttgagcttcc gttgtcatct gcgccgcaag cgcctaacac
     3181 tagagctgaa aatgctaata cggcaaatcc agttacaaaa cgttttttct tcattttaat
     3241 accccaccat tccatttcta gtccacaatt tttctttgtg attcattata cattctaatc
     3301 tttttagcct ataagtcaac tatttttgat aaataaccca tgtaatattt caaacgtttt
     3361 ctttcagcaa atttcgtgaa aaaacgcttt attttaaaat ttgaaaaaaa tccaaaagga
     3421 gttagacaaa agaacagaac atctatataa tatatggaga ttgtgtaata agtatcaaac
     3481 taaaagaatt atcatatatt cattatatag atagtctata aggagcggat gatcaatggc
     3541 aaagcaaaat attgtcgttg taggagcagg atatgccggt gtttcagcta caaaatactt
     3601 ggcaaagaaa ctgaaaaaag aagatgtagc aattactctg atcgatcgtc attcgtacca
     3661 tactatgatg accgagttgc atgaagtagc tggcggacgt gtagaaccag aagcaatcca
     3721 atacgatctg cagcatttgt ttgctcgtca aaagaatgta caacttgtca cagacactgt
     3781 aatcggtatc gataaagaac gaaaaatcgt caagaccaaa ttaggttcct atccttttga
     3841 tcagctgatc cttggaatgg gcggagagcc gaatgacttt ggtacacctg gtgtcaaagc
     3901 acacggattc accctttggt catttgaaga ttctgtaagg attcgggaac atattgagaa
     3961 gacagtagca aaagctgcat tggaaccaga tgatgaactg cgtaaagcca tgctgacttt
     4021 cgttgtctgt ggttctggat ttaccggtat tgaaatgatt ggggaactga tcgactggaa
     4081 aaatcgccta gccaaagatt ataaattaga tccaaacgaa atcacactga tggtcgtaga
     4141 ggcaatgccg acgatcttga atatgttgga tcgaaatgat gctgcaaaag ctgaacgcta
     4201 tttgcgtaag cacaacgtgg aattattatt gaatgcacca atcgtagaag tggctgctga
     4261 tcatatcaaa ttgaaagacg gacgcaacat tccgactcac acactgattt ggacagctgg
     4321 tgtcaaagga acttctgacg ctgctgactt tggattagaa tcagcacgcg gacagcggtt
     4381 gatcgccaat gaatatatgc aggcaaaagg ctacgaagat aaaaacatct atatcattgg
     4441 tgatttagtc ttttatgaag aattcccagg aacaccgaca ccgcagatcg ttcaagctgc
     4501 tgaacaaaca ggacacacag ccgcagcgaa tatcgtggct tcgatcaaag gcggagaaaa
     4561 gcataaattt aaaggaaact atcaaggatt tatggtttct attggtgcca aatggggggt
     4621 agctaaccta tttgataaga tccatttaag cggattttta gctatcgtaa tgaaacatat
     4681 cgtgaatttg aaatacttct ttgatattcg ttctggttat tatatgttcc aatatttaat
     4741 gcatgagttt ttccatatca aagatgatcg caacgtgact cgaggacatt cttctcgtta
     4801 cggcaatgtc ttatggagtg tgccacttcg tattttctat ggtttagttt ggttcgttga
     4861 agcttccaaa aaaatcattg ggaatggtga agtgttcaaa ccaagtactt ggtttggtga
     4921 aggatcatgg ttcactggaa acagtgtgtt cccttggaca tgggaataca atgtaacaga
     4981 tgttgccagt ggtgcttctg ctgctggtga tgcaaccaca gctgccagcg gagcaggggc
     5041 aagtgccgga gaagcagcga ctcaagctgc acattttggt ttgagttatg catacggtga
     5101 agaaccaatg gctgtgattg agaagtcacc agactggttc acaaagatca tgcaagtgat
     5161 catgccaaac aatgatgtag caatgttctt ccaaaaattc atggtattct ttgaaattgc
     5221 cttggcatta gcattgattg ctggattatt cacatggcta tgcagtgcga caacgattgt
     5281 attggtgatc acgttctgct tatcaggcat gttttactgg gtaaacattt ggttcatttt
     5341 tgttgctttc gcattgatga acggttccgg acgtgcgatt gggttggatc gctgggtaat
     5401 tccatggcta caacgtacac taggtaattg gtggtacggt agaccaagtt cccagtacgg
     5461 caagaaataa atagataaag aacgttattt gaatgcaaga tttcaataga aaggggctga
     5521 gacaaaccgt cacagcctcc tttttatgta aaattaaagg aataagatgc ccgagataag
     5581 cctccatgag caagacacag gtgtcacaac ctctgttaca acctcttgtt ataaacggtg
     5641 ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaacttg aaaagtaatt
     5701 ttcgtaaatt ccttcttatt tcttgaagtt gcccacttct gtcacaacct cttgttataa
     5761 acggtgttcc acctgctgct tctcggtaat aagcccaaag aattagaaaa catggaaagc
     5821 tgttttctat tcttcgttct tattactcaa agcaagacac aggtgtcaca acctcttgtt
     5881 tttatgctaa aattgggtga gttaatctgg aaaaaggaga acaaaaatga gccttaaaga
     5941 atttgctgaa aaaagtctgc tcaaaccttg ggatgttttt attattttgc ttttgactgt
     6001 tctttctttt ttaccagtca tgatttttgg tcttcagcaa aacaccgcaa cagcagaaaa
     6061 agaagcggta ctgcgtgtgg atggcacaga aatcaaaaca ttccctttga tggctggaga
     6121 agagagcaac acatatactt acaaagatga acacggagat tataacttga tcgagataga
     6181 tggcgatcga gtacggataa aagaagcaga ttgcgatgac cagatctgcg tacgtcgagg
     6241 ctgggcaacg aaaaatggcg aaaccatcgt ctgtttgcca cataaattag tgatcgaagt
     6301 ccgttcaaca gatggaggtg acgaagacag tctgatttat tagaccttca cacaccatga
     6361 gtaaattaca aaaaatgatt tatatttcgc tacttgttgc acaaggagtg atcattggtt
     6421 tattggaaaa tatgatccct tatccttttt cttttgcgcc tggtgcaaaa ctaggcttag
     6481 ccaatctaat cacgatcgtt gccattttta ccatgaaaaa acaagacagc ttcttactac
     6541 tttggctacg actattcctg actactttgc taggcggaac gatttccaca ttcctttata
     6601 gtatgagtgg tgctttgctt agttattttg gaatgttatt ggtcaagcaa ttaggaccaa
     6661 aacgtgtcag tatcatcgga attagtgcgg ccggcggttt catgcataat gtcggacagc
     6721 ttttgatggc ttctttcatt gcacaatcat ggacagtcat gttgtacttg ccagtccttt
     6781 cagttttagg gattttatca gggattgcaa tcgggatcgc agcgaattat cttttacgac
     6841 atgtgcagac acttcaacga ttccaaaaag attatgagca gggaaccaaa caaaaatgga
     6901 gcccaatgtt taccaaaaaa taatcgtcac acaaggagtt tttttatgaa gctacatcca
     6961 atgtgggaaa actatcctgc tttaagtaaa gaattgacag aaacactaaa acggatggaa
     7021 gaaactgtcc gcctgaaaaa caaacctgtc gaacaagcca tcaaagaaac gatccatgcc
     7081 ggcggaaagc tgttgcgtcc tgcctatcag ctattgttct cacaatttgg accagagcaa
     7141 gatcgagaca aagccatcgc tttggcagct tctatcgaaa tgctccatac ggccacactg
     7201 atccatgatg atatcgttga tgaagcggat ctgcgacgtg gtcagcctaa tatccgtagt
     7261 cagtttggta atgtcgtttc tgtctatgca ggagactatt tatttgtctg ctgttttaaa
     7321 ctattgtctg attactccac ttccttgaaa agtctccaat tgaattctcg cagtatggaa
     7381 aaagtattga atggcgaatt agggcagatg gatgatcgct ataattatga actttctgta
     7441 aaagaatatt tggaaaatat ttcaggtaag acagctgaac tgttccaatt aagttgttct
     7501 gttggcgctt ttgaaagtgg aacaagcgag cgcttcgcaa aaaaagcagg agatatcgga
     7561 ctttctatcg ggatggcctt tcagattatc gatgatattt tagattatac aaaagagagc
     7621 caggaaatcg gcaaacctgt actagaagac atgcgtcaag gggtctattc tttaccgttg
     7681 atctactctt tgcaaaaaaa caaaccaaag ctactacctt atttagagaa gaaagcggca
     7741 atgacagaag aagatgtcga cgccgtacgc aaaatcgttg aacatacagg aggcgtggaa
     7801 gaagctagga agttagcggc ctcttacaca gaaaaggctt tgaaggaaat aaagaaatta
     7861 cctgcc
//
LOCUS       DACGLH010000110         7827 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.116, whole genome shotgun sequence.
ACCESSION   DACGLH010000110 DACGLH010000000
VERSION     DACGLH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.116"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..1253)
                     /locus_tag="IXT21_002675"
     rRNA            complement(<1..1253)
                     /locus_tag="IXT21_002675"
                     /product="16S ribosomal RNA"
     gene            complement(1939..3444)
                     /locus_tag="IXT21_002676"
     CDS             complement(1939..3444)
                     /locus_tag="IXT21_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4870037.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(3441..4127)
                     /locus_tag="IXT21_002677"
     CDS             complement(3441..4127)
                     /locus_tag="IXT21_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4870038.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(4320..5741)
                     /gene="gndA"
                     /locus_tag="IXT21_002678"
     CDS             complement(4320..5741)
                     /gene="gndA"
                     /locus_tag="IXT21_002678"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ4870039.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(5911..6090)
                     /gene="rpmF"
                     /locus_tag="IXT21_002679"
     CDS             complement(5911..6090)
                     /gene="rpmF"
                     /locus_tag="IXT21_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ4870040.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(6148..6717)
                     /locus_tag="IXT21_002680"
     CDS             complement(6148..6717)
                     /locus_tag="IXT21_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ4870041.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            6903..7805
                     /locus_tag="IXT21_002681"
     CDS             6903..7805
                     /locus_tag="IXT21_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4870042.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
BASE COUNT         2137 a         1748 c         1371 g         2571 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat caaaaccatg cggttttgat tgttatacgg tattagcacc
     1081 tgtttccaag tgttatcccc ttctgatggg caggttaccc acgtgttact cacccgttcg
     1141 ccactcctct ttttccggtg gagcaagctc cggtggaaaa agaagcgttc gacttgcatg
     1201 tattaggcac gccgccagcg ttcgtcctga gccaggatca aactctcata aaaagttcga
     1261 acaatcttgc gattgttaag ctcaatttgt ttgctagcat attgcttgct tgttaaaaat
     1321 gtttgttgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
     1381 gttttcaaag gtctaatctt tttgttttaa caactcttta atcttatcag attcagaaga
     1441 gaatgtcaac aacttctttg tttatttttt ctaaacgtct ttgccactca ttagcagcga
     1501 cttgattatc ataccatctt tattgttatt tgtcaataac tttttcgaag tttttttcga
     1561 taaaatcaag ctttattagc tatctcatcc aaacgacttc gttattataa catgacatca
     1621 aaagtgcgtc aacactattt tttataattt ttagtgacac cttaaaggaa tcatttagaa
     1681 caaataataa tatagcaatc tctttataaa attgcaatag aaaattccct atttttattt
     1741 attattttta aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac
     1801 ttacatttaa ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca
     1861 tccctgaatt tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa
     1921 acaagtaaag cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa
     1981 aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag
     2041 ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc ttgctttatc
     2101 cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa
     2161 gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt
     2221 tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg
     2281 attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata
     2341 gtctggatat aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt
     2401 tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg
     2461 aactaagctt ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat
     2521 ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac
     2581 aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa
     2641 acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt
     2701 gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc
     2761 tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact
     2821 taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc
     2881 atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt
     2941 agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg
     3001 aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt
     3061 ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga
     3121 atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc
     3181 atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt
     3241 agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac
     3301 aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat
     3361 gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt
     3421 ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg
     3481 atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc
     3541 actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt
     3601 gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt
     3661 agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg
     3721 cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc
     3781 aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca
     3841 tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata
     3901 atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct
     3961 gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca
     4021 gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca
     4081 aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc
     4141 ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat
     4201 ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa
     4261 tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat
     4321 tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta
     4381 tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg
     4441 tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca
     4501 gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa
     4561 tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc
     4621 aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc
     4681 aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc
     4741 atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc
     4801 gtgaactcaa aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta
     4861 taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag
     4921 tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc
     4981 acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg
     5041 atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt
     5101 tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc
     5161 atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg
     5221 ccaatatatg tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt
     5281 ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa
     5341 gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac
     5401 tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg
     5461 cctttatcaa ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg
     5521 accattagca agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat
     5581 gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta
     5641 cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc
     5701 ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac
     5761 gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa
     5821 caacatattt tttatttacc gactacaaaa aaataaacct caaggctcca tggttagcct
     5881 tgaggtttta cattttctaa ttaaaatgta ttaagcttct tttgacatta cgtccttacc
     5941 atcatagtaa ccacaatttg cacatacgtg atggcttttt ttcatttcac cacagtttgg
     6001 acattcgttt aaccctttga ttgtcaattt gtagtgtgta cgacgttttg cttttttagc
     6061 ttttgatgtt cttctagctg gtactgccat tttgctacac ctccttgtaa agtcttgtat
     6121 ctaaattaga cacaatatat tccaatctta cttgttatcg tcgtcttttg atttagaatc
     6181 aaaaagttca gatagttttg caagacgagg atcaatttta ttctcggctt cttccatttt
     6241 tctgcgctgg tattcttctt cggaaacaac ttcccaatca ttgccttttg gaagttctgt
     6301 tgctgcttcc tcttcttttg tcaatacccg cattggaatc gccaataaga tgttgtcttc
     6361 aatcgactct gctaaattca ataatggacc ttcaattagc aaaatctcat ctgcagacat
     6421 ttgttcatct ctactttgga attgttcttc tgtcatgaat acttcgtcta cagaaaaatc
     6481 catgggcagt tcaacaggtt ctagtgaacg tgttgaagga actgtcaaag tcgtctgcac
     6541 agtgtaatga acgatatagc ctttcttatc aactgagacc aaaccttcga cttccactgg
     6601 cgcaacatca agtattaaat tgtctcgctt catgagagcg gctttcaagt cgagcgtctc
     6661 atgaaaagtt aacggtgtct cctggtattt tctcaattcc aataaagacc atttcatttt
     6721 tcatcactcc taagcaacaa aagcaattat acagggcaaa aagcatgttg tcaatgaaat
     6781 tttcttgaca ccttgcagtt cataaaaaaa cagaaaaatc tttattagaa agcctctgtt
     6841 tgctatcatc ctcagaatca tttacaataa tcattattcc attaaaggag cggatgatct
     6901 tcatgaacac gaaagatatc gaatactatg tccaaatcgt caaacaaaaa aattttacga
     6961 aagtggctaa ttatttccgt gtgagccaac ctactattac atatgcatta caacgtcttg
     7021 aaaaagagtt tgacaccgta cttattacaa gagatcgatc gcatcacgaa ttgatcgtca
     7081 cgccttctgg aaagcagctg tttctccatg cacaaaccat tctccaagag atttctactg
     7141 caagagctga aatcactcag ttgcaaaaga aaaagcttcg ttttggcatg cctcctatta
     7201 tcggcaatga atactttcct aaactgtcaa gttactttat caagcatgaa ttgatgaatc
     7261 aaattgagat taccgatgga ggctctcggg acttatacgg gatgatccga caagggagga
     7321 tcgatttagc tgtattaggc tcaacacagc caatcgtgga tcaatattta gaaacagagt
     7381 tacttttgga caaacgtttc atgatcgtcg tgccacctac acatcctctt tccaagcagg
     7441 atactgtctc tttcaaagat atagcagatg aacagtttgt actattgaac gaacattatg
     7501 tccaccccgc agcattcact aagctttcta aacaaacaca tactcatcca aaagttgttt
     7561 atcaaagcaa tgacttgacg attttaaaga gtatggtccg agaaaatgtg ggtatcggtt
     7621 ttttggcaga gattgctgtc catcctgaag atcatttagt ttgcctccct ctttcagatg
     7681 aagttcagcc tcgcttcttg atttcattgg caaaacgaac acagcagatc gtcacgcctc
     7741 ttcaagaaca agtagtccaa gttatccatg aattcgttac agcacaaaaa aacagctctt
     7801 cgtaaaagaa gagctgtttt tattttt
//
LOCUS       DACGLH010000111         7760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.117, whole genome shotgun sequence.
ACCESSION   DACGLH010000111 DACGLH010000000
VERSION     DACGLH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.117"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXT21_002682"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXT21_002682"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ4870043.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IXT21_002683"
     CDS             complement(2032..3273)
                     /locus_tag="IXT21_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ4870044.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IXT21_002684"
     CDS             complement(3291..4214)
                     /locus_tag="IXT21_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ4870045.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IXT21_002685"
     CDS             complement(4435..6927)
                     /locus_tag="IXT21_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ4870046.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IXT21_002686"
     CDS             complement(6934..7401)
                     /locus_tag="IXT21_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ4870047.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IXT21_002687"
     tRNA            complement(7537..7610)
                     /locus_tag="IXT21_002687"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IXT21_002688"
     tRNA            complement(7668..7741)
                     /locus_tag="IXT21_002688"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DACGLH010000112         7615 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.118, whole genome shotgun sequence.
ACCESSION   DACGLH010000112 DACGLH010000000
VERSION     DACGLH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.118"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(14..706)
                     /locus_tag="IXT21_002689"
     CDS             complement(14..706)
                     /locus_tag="IXT21_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4870048.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IXT21_002690"
                     /pseudo
     CDS             791..967
                     /locus_tag="IXT21_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IXT21_002691"
     CDS             complement(1143..1712)
                     /locus_tag="IXT21_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4870049.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IXT21_002692"
     CDS             complement(1786..2109)
                     /locus_tag="IXT21_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870050.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IXT21_002693"
     CDS             complement(2263..3075)
                     /locus_tag="IXT21_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870051.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IXT21_002694"
     CDS             3429..4277
                     /locus_tag="IXT21_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ4870052.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IXT21_002695"
     CDS             4422..5363
                     /locus_tag="IXT21_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ4870053.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IXT21_002696"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IXT21_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2267 a         1508 c         1373 g         2467 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 tcccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt ttttt
//
LOCUS       DACGLH010000113         7351 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.119, whole genome shotgun sequence.
ACCESSION   DACGLH010000113 DACGLH010000000
VERSION     DACGLH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.119"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(58..354)
                     /locus_tag="IXT21_002697"
     CDS             complement(58..354)
                     /locus_tag="IXT21_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ4870054.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            709..1065
                     /locus_tag="IXT21_002698"
     CDS             709..1065
                     /locus_tag="IXT21_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ4870055.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1075..1974
                     /locus_tag="IXT21_002699"
     CDS             1075..1974
                     /locus_tag="IXT21_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ4870056.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2491..3222
                     /locus_tag="IXT21_002700"
     CDS             2491..3222
                     /locus_tag="IXT21_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ4870057.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3367..5394
                     /locus_tag="IXT21_002701"
     CDS             3367..5394
                     /locus_tag="IXT21_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ4870058.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5502..6599
                     /locus_tag="IXT21_002702"
     CDS             5502..6599
                     /locus_tag="IXT21_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ4870059.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6906..>7351
                     /locus_tag="IXT21_002703"
     CDS             6906..>7351
                     /locus_tag="IXT21_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4870060.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         2555 a         1181 c         1462 g         2153 t
ORIGIN      
        1 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
       61 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
      121 gggaagctta ctccaccgtt ttaataattg atagaaataa ctctcttctg ctttgatttt
      181 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
      241 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
      301 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
      361 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
      421 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
      481 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
      541 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
      601 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
      661 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
      721 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
      781 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
      841 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
      901 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
      961 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
     1021 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
     1081 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
     1141 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
     1201 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
     1261 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
     1321 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
     1381 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
     1441 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
     1501 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
     1561 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
     1621 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
     1681 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
     1741 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
     1801 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
     1861 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
     1921 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
     1981 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
     2041 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
     2101 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
     2161 atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat tgattgaagt
     2221 ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc
     2281 tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa
     2341 atcgggctga taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc
     2401 ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata
     2461 tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca
     2521 gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac
     2581 ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact
     2641 cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca
     2701 agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa atttgggact
     2761 caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg
     2821 tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa
     2881 ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca
     2941 cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa
     3001 acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct
     3061 aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat
     3121 caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc
     3181 actaaattag tttggacatt agaagataca cctaacaact agtttactga aaagaactgt
     3241 taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc
     3301 taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa
     3361 gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc
     3421 tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac
     3481 gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa
     3541 agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag
     3601 ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat
     3661 cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata
     3721 ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt
     3781 tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta tttatactta
     3841 ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga
     3901 atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct
     3961 actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa
     4021 aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga
     4081 catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc
     4141 gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg
     4201 gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt
     4261 atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca
     4321 acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca
     4381 tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga
     4441 aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag
     4501 aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc
     4561 aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat
     4621 ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac aactttgcag
     4681 gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg
     4741 attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat
     4801 gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg
     4861 aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg
     4921 gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac
     4981 agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa
     5041 atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat
     5101 gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga
     5161 gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg
     5221 acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt
     5281 ttagattcta cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc
     5341 ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa
     5401 gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt
     5461 gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt
     5521 tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta
     5581 acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga
     5641 atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag
     5701 aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga
     5761 atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag
     5821 acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa
     5881 aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg
     5941 gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa
     6001 ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc
     6061 aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa
     6121 tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc
     6181 aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg
     6241 aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg
     6301 gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg
     6361 aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg
     6421 tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg
     6481 ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt
     6541 ttattttatt atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag
     6601 gttaagataa caaatagtaa atttttatta ttgtaggatg gttgggctct ttttataaca
     6661 atataaaata taccatttta ttaataaaaa tgcatactag taaatgatat aattctaata
     6721 acaggtttga tcgcgagaaa ggccaaataa aacgctctcc aaaatgttaa aaaaaagaca
     6781 ggacgatgta tattatacaa acaagaatcg ctgacaacag tgactcggat ccaggaaatc
     6841 agttttatcc ccagctagtt ggtgaatgca tggaaagtaa tatacgtaga aagagctgat
     6901 gactaatgaa ttatgagttt tttttaagca aagaagatca cagaagttat tcattactta
     6961 aatatttaga agcaagctca aatttatcag agtcaatatc gaatgttcaa gaagagttgt
     7021 cgctatcgac atttctcttg aaaaaaacga tcgataagct gaaacatgat ttacaaaagt
     7081 tcgatctaga gaaaaatttg cgagtaaccg tcagtgaatt ggatatatgt ctggaaatta
     7141 atggaaaatg ttctagcaaa gctctgttat ctcgttatat aacggattct ttatctatga
     7201 aaatggtatt ggctttcttt caggaaaaat atatctcagt agaaaatttt gctaaacagc
     7261 atcacgtgag ttattctgtg gcttataaag tattacaaag actaaaaaga aatctaaaaa
     7321 aatatcaaat tttttttgaa aagagaaagt t
//
LOCUS       DACGLH010000114         7324 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.120, whole genome shotgun sequence.
ACCESSION   DACGLH010000114 DACGLH010000000
VERSION     DACGLH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.120"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..387
                     /locus_tag="IXT21_002704"
     CDS             <1..387
                     /locus_tag="IXT21_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAQ4870061.1"
                     /translation="QSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILN
                     AIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYF
                     GKDEDVVYLINQIENYTKILYAEVSK"
     gene            384..2177
                     /locus_tag="IXT21_002705"
     CDS             384..2177
                     /locus_tag="IXT21_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ4870062.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            2377..2691
                     /locus_tag="IXT21_002706"
     CDS             2377..2691
                     /locus_tag="IXT21_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4870063.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            2712..3089
                     /locus_tag="IXT21_002707"
     CDS             2712..3089
                     /locus_tag="IXT21_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4870064.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            3099..3872
                     /locus_tag="IXT21_002708"
     CDS             3099..3872
                     /locus_tag="IXT21_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870065.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            3894..5297
                     /locus_tag="IXT21_002709"
     CDS             3894..5297
                     /locus_tag="IXT21_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ4870066.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            5479..6663
                     /locus_tag="IXT21_002710"
     CDS             5479..6663
                     /locus_tag="IXT21_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ4870067.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            6660..6950
                     /locus_tag="IXT21_002711"
     CDS             6660..6950
                     /locus_tag="IXT21_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870068.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            6947..7168
                     /locus_tag="IXT21_002712"
     CDS             6947..7168
                     /locus_tag="IXT21_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870069.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
BASE COUNT         2552 a         1159 c         1394 g         2219 t
ORIGIN      
        1 caaagtgaac aatatttaga aagaaattta gcgatgttaa aactagccaa tcatgttggt
       61 aaggggtggt ttgcaattga gttaggtgaa aaaattgact ttttaatcaa aattcctaag
      121 tatattctta atgctattgc ttttgcatgt caggaaatag tagatctctc aatttataaa
      181 aaaattctac tccatacaat cgacttgtac gaggaaacta cagaattgtc agctttatca
      241 gctagtttaa aaacaagtga tacagctgat ttgaatatag caataatttc tttattagag
      301 tactttggta aagatgagga tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa
      361 atactttatg ctgaggtttc aaaatgacaa atatcttcga aaaaatcgta aatgatttaa
      421 atttagaaca aaaagatgtt gtggaaagtg aagataacct ttatgtaaat gcatgtcctg
      481 ggagtgggaa aacaagggta cttacaagga aaattgctta ccaagcaatt aaacacgatg
      541 gatcactaaa aagaataatt gctatcacat acactaatag agctgctgaa gaaattaagg
      601 aacgcctaag tttacttagt atagatgatg atattaatat ttgggtgggg acaatacatc
      661 agttttgtct tgaatttatt atttatcctt ttaaaatgaa tctctcaaga gtatcaaaag
      721 gatttacaat tattgatgat tatactcaaa gagcttacgt cagccaaatt aataatatgc
      781 tgaatttaaa tctcaaaacg tatgaactga acaaaattga tttgtcactt tctcatgatt
      841 ttaaaattaa tgaagttcag tatactcaag tttctgaaat gtatcacact atgttggaaa
      901 aaaataaaga aattgatttt gatatgattt tactagtagc gtataaaatt ttaactgtaa
      961 acagtaacgt tgcacgtaat attgctagta atataagggc gatttatgtg gatgaatttc
     1021 aagatactag agaattacaa tacaatgtaa tagctaaatt acttcaaaac aaccctcaaa
     1081 ttcagtcaat gtttgttgga gatattgacc aagcaattta tggatcatta gatggtatag
     1141 ctaaatcagt tgaagaacta gaaatgctaa ctagtcacac ttttcaatca aagacgctac
     1201 atggctgtta caggtctaat cagagattag tagacttcta ctctaatttt cagagttgtc
     1261 cttacgagat tgaaagcaga gggaataata aaaacgaccg tggttttata agttatgatt
     1321 gcaaagttac taaggaggat ttacccaaca taataaaaaa tataataaag gaaaagattg
     1381 aaagcggcat tcatgaaaaa gatatttgtg tacttgctcc tcaacactat cctctgtttt
     1441 cactgggtga gtatttaaag aaagaattac cgtattgcaa ttttgatgct ataaatatat
     1501 caccaattaa ggtaaataat catagtttat ttttcaagtt agcaattttg tactttactg
     1561 aatcaggtga aaaggttcga agaagaaagc taattgcaaa cgatatttta attatattaa
     1621 aaaatgaatt tggaatagaa gttaaagatt attttattga cttagattta ttacagctaa
     1681 taaacagtgt gaaaagaatg tatagtggag acgacaacgg tgttgattta tacaaatttg
     1741 ttaccaaaca tatatttgtt aatcttaata ttgacgagaa aaattacccg tctattttta
     1801 agcactatct attttttatt aacgaagtag aagatcggat aaaaaataat tcattatcta
     1861 gtagtgtatc tagttttaag aagatgttta aatctcgaga tggtatcaca cttactacag
     1921 cacataaagt taaaggaaat gagtatgata ctgttatcgc tattaatctt ctaaacggga
     1981 aaattcctca ttggaatgaa atttttaatg tttctgataa aggaattagt tcagcgagaa
     2041 aactactata cgtaatttgt tcaagagcaa aaaataattt atttcttttc tcagaggtgg
     2101 gttattttac gagtaaagga gacttgcttt atcccactca attattagat aaaatagaat
     2161 ttgattataa tatttaaaat acctaggtag tcgttgtttt aaatatagta ggaatcggac
     2221 gctcagtacg gagcgtctat ttttttaccc aattttgaaa ggaagtgaac ttatgaaaat
     2281 aaaaaatcaa gaatcaaaag gtcgctctcc cccctttaaa accatcacac aaataagatt
     2341 cattcaaaca taaaaacaga aaggataggt aaaaatatgg aacttaaata tgtcattccc
     2401 aatatggaaa aaacatttgg acaattagaa tatgctggtg aagggaacat tgaacaacgt
     2461 cgaataaatg gcagaccaac cattctctct cgtagttaca atttatactc aacgattcaa
     2521 cgagcagatg atattgttgt cattcttccc tccgaagctg gcgagaaaca ttttgaatcc
     2581 gacgaaaaag tcaaactcat caacccacat attaccgcag aaggttataa aattggcaat
     2641 cgaggattta ccaattacat tatgcacgca gacgatatgg tgaaagcata agaaaggaaa
     2701 ggtgaaaaaa tatgagatta gcagaaggta ttgtgattga aaaagaccag acatttggca
     2761 cattgaaatt ttccgccttg cgtcgtgagg ttcgtatcca gaatgaagat ggtacggttt
     2821 ccgaagaaat taaagaacgt acctatgact taaaatcaaa aggacaagga cgcatgatac
     2881 aagtcagtat ccccgcttct gttcctttga aagaatttga ctacaacgca gaagtggaat
     2941 taatcaatcc tgtggcagat accgtagcga cggctacttt tcgaggggca gacgttgatt
     3001 ggtacgtgaa agcagacgat cttgtattaa aaggacagac aaaacctcat cagaaatata
     3061 aaaaagaagc gtccgaaagt gaaaaatagg tgttgcttat gacaaacaca ccattgattt
     3121 tgaaagaaat tccgaaggat gaagccattt cttttattcg gcaataccat tattcaaaga
     3181 tactccctag attgtgtaaa tactttctag ggattttttc agaagaaaaa ttattaggcg
     3241 ttgtggaatt aggctggggt acgcaacccc tacaaaccat acgcaaactc tttcccgaca
     3301 gttcccttca aaccacagat tatttagaaa ttggaaagat gtgttttctt ccagaaatga
     3361 atcaaaccaa ttattttggc agtcaagctc tttcagctct aattaagtgg ttgaaagaac
     3421 atacggactg tcatttttta tatacactgg cagatggaat tgagggaaaa tgtggctatg
     3481 tctatcaagc gtccaacttc ttttactgcg ggtatttcaa gacaagtgtc tatcgtgata
     3541 agcagtcatg ggaaaagata caccctcgaa gtgctcgcct tttattagag gaaaatgctc
     3601 gctttgaaca ggtagagaaa aagcattggc tttcccaggc attttgcgaa tacaaaggga
     3661 ttgagaaaat caatggacgc atgtttcgct atctctatcc gttgacaaaa gaagcgaaaa
     3721 aactgttagg acacactctc tatcgacgtc attattatcc gaaagaaaaa aatttacgct
     3781 ttgaaaagcg aatcgcttat cagaaatatg aagccatttc tcaaccaact tttgataaac
     3841 aggctcgtat ctacaataca caattatttt gataggtgga aaggaggatt tttatgccat
     3901 tgcttcatga cagaggagag cgaatcaaag ccaacgataa gcacttggtc tatcattttg
     3961 tactaatttc attgctggta gtttttctag tcgtcctact cccttttcat tggagagcgt
     4021 tacaatcggt gaactggcaa gcagttcagt tagatacatt tttctatcaa cttcatacgc
     4081 catacttttg gagcagtatt ctcactgctc tttttgtatg cggacttatc agctggcttt
     4141 attatcacta tcgtatagat tctataaaac agttagagca ccgtcaaaag ttagctcgta
     4201 tgattttaga aaatcattgg tatgaagccg aagaaactca atcagaggga ttctttaaag
     4261 atttacccag caagaaaacc aaacaacgaa tccgccattt tcctaaaata tattatcgaa
     4321 tgaaagatgg actaatccat attcatgtag aaatcacctt agggaaatat caagatcagc
     4381 ttttgcacct agaaaacaaa ctagaaacag gattgtattg tgaattagtg gaaaaaacgt
     4441 tgaaagattc ttatgtagag tatgtcctac tttatgatac tattggcaat cgaattggca
     4501 ttgaagacgt ggtagctcaa gatggacgtg tcaaattgat ggaatcggtc tattgggagt
     4561 tcgattctct ccctcacatg ttaattgctg ggggcactgg tagtggaaaa tcctatttta
     4621 ttctcacatt gattgaatct ctcctgcata caaatgcaaa gctttatatt cttgacccta
     4681 aaaatgctga tttggcagac ttaggggcgg tcatgaataa tgtctattac cgtaaagaag
     4741 atatgttggc atgtatcaat caattttacg aagatatgat tgatcgcagt gaaaccatga
     4801 aacaacaccc taactataaa acaggagaaa attatgctta tcttggtttg tctgccaatt
     4861 ttcttatttt tgatgaatat gtggcaatga tggatatgct aggtcgtgaa agtaccaccg
     4921 ttattcataa actcaaacaa attgtaatgt taggacgaca agcaggcttc tttttaattc
     4981 ttgcctgtca acgtccagac gcaaaatatt ttagtgatgg gattcgggac aactttaact
     5041 tccgtgtggc tttgggtcgt atgtctgaaa tgggctttgg tatgatgttc ggaagcgata
     5101 cacaaaaaca atttttctta aaacccatca agggaagagg ttacgtggat acaggaaaaa
     5161 gtgtgattag tgagttttat acaccccttg taccaaaaag gtacgatttc ttaggagaaa
     5221 taggaaaagt cattcaaaaa aagcaatctg agcctgtacc gcacgaagtg aaaggtacag
     5281 gttcagactt gctttgattg gtgtggcgtt agccacgcca atcctttagc cccctctatc
     5341 taacaggggg gcacaaatac aactgaaaac aggcaattct catcaaccaa tgttgatggt
     5401 acaaaggatt gcggaaaata tgaaaggtgt aaactcaagg gtcatagttt acatcttttt
     5461 tagattggag gtttgcgaat ggaaaacgaa aaaatttgga ttaaggaatt gaaagaaaag
     5521 cgactggctt atggcgtttc tcaaaataaa ctggcggttg cttctcatat taccagacct
     5581 tacctatcgg atattgaaac aggcaaggca gtccctactc aacaggtgaa agaagattta
     5641 ctgaacgctt tggaacgctt caatcccgat aacccattgg agatgttatt cgactatgta
     5701 cggattcgat tccctaccaa tgatgtggct caaattatcg gtgaggtttt acgtttgaat
     5761 atggattata tgctacacga agactttggc tattattctt atcccgaaca ctatcgtttc
     5821 ggtgatattg ttgtcctagt ctctcatgat gtaagtaaag gtgttttatt agaactgaaa
     5881 ggaaaaggct gtcggcaatt tgaaaacttt ctattggctc aacatcggag ctggtatgat
     5941 ttctttcaag attgcatgga acataaaggt attttcaaac gcttggattt agctatcaac
     6001 gataaaacag ggattttgaa tattccagat ttggcaaaga aatgtaaaca ggaagaatgc
     6061 atttccgtct ttcgaagctt taaaaattat cgcagtggtg aattggttca ccgtgatgaa
     6121 aaaccataca tgggaaacac actttatatt ggctcattaa aaagtgaagt ctatttctgt
     6181 atatatgaga aagattatga gcaatacgtg aaaaatgatg ttcccttaga agacgcagaa
     6241 gtaaagaatc ggtttgaaat tcggttaaaa aatgaccgag ccacccaagc catgaaagat
     6301 ttattggcac atcaacaggc ggaaaaaaca gcctttgaaa ttatcaatcg ctatatccgc
     6361 tttgcagata aagacgacac taaacgtcgg agtgattgga aaactaatga acgttgggaa
     6421 tggtttatcg gtaaaaatcg tggagcatta cgcctaacca cacagccaga gccttattct
     6481 tttgaacgaa cattgaactg gttacatcat caagtcgccc ctacattaaa aatcgcaagc
     6541 attttagatg ttctcaacgg tacaaccatt atctccacca tgattcagga agccaaactg
     6601 acagaaaaac atgagaaatt aattgaacaa caacatctag ccatggagga tttgattaca
     6661 tgagcctttt aacaaaagaa ttaaagaagt tagggtttca gtgtggcatt gagtttcaag
     6721 cgtatataca aaatacaggg aaatatacgt ctctcattat tgagggaaaa cggcaagctg
     6781 gtgataccat ctatacctat gatttttaca aggtgagatt ttataataat tataccaatc
     6841 gagtaaccgt ctatggagag catttaaccc cttttcaatt attaaggcgt gtaaaaagct
     6901 atatctatta tcgagaaaaa tatcttaagg aaaggaggac aatcacatga attttgggca
     6961 gaatttgtac aactggttct tggataatgc acagtcattg gtattgttag cgattgttgt
     7021 cattggactg tttttaggtt ttaaacgaga attttcaaaa ctcattggct tcttgattat
     7081 tgctctaatc gccgtcggac ttgtgttcaa tgcagctggc gtgaaagatg tgttattaaa
     7141 cttatttaac cgaatcattg gtgcttaaca gatatttttc atgataaaat aggaagtata
     7201 tattttgtta tgaaaggatt tatgaatatg tttacaaatg atcaatttaa atctctttta
     7261 attaaaacat tagaaagaaa gagctcttca aattattatg aaggaggtaa cgagattgtt
     7321 tcta
//
LOCUS       DACGLH010000115         7307 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.121, whole genome shotgun sequence.
ACCESSION   DACGLH010000115 DACGLH010000000
VERSION     DACGLH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.121"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(561..1601)
                     /locus_tag="IXT21_002713"
     CDS             complement(561..1601)
                     /locus_tag="IXT21_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ4870070.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1950..2276)
                     /locus_tag="IXT21_002714"
     CDS             complement(1950..2276)
                     /locus_tag="IXT21_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870071.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2263..3051)
                     /locus_tag="IXT21_002715"
     CDS             complement(2263..3051)
                     /locus_tag="IXT21_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4870072.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3419..3709)
                     /locus_tag="IXT21_002716"
     CDS             complement(3419..3709)
                     /locus_tag="IXT21_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ4870073.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3845..4024)
                     /locus_tag="IXT21_002717"
     CDS             complement(3845..4024)
                     /locus_tag="IXT21_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870074.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4021..4371)
                     /locus_tag="IXT21_002718"
     CDS             complement(4021..4371)
                     /locus_tag="IXT21_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870075.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4364..5689)
                     /locus_tag="IXT21_002719"
     CDS             complement(4364..5689)
                     /locus_tag="IXT21_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ4870076.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6139..6753
                     /locus_tag="IXT21_002720"
     CDS             6139..6753
                     /locus_tag="IXT21_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4870077.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6918..>7307)
                     /locus_tag="IXT21_002721"
     CDS             complement(6918..>7307)
                     /locus_tag="IXT21_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870078.1"
                     /translation="KEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNF
                     FYKINDSFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYK
                     ELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         2236 a         1313 c         1074 g         2684 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 tttgtatttt caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatat cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatatgcaa acactagttc
      361 cctatttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg
      481 taacatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta
      541 tagtgatcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa
      661 ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt
      721 tgataagctt tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc
      781 agttcttcat aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga
      841 atagtttgga cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt
      901 aatacatttt ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta
      961 tctagtaaaa cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg
     1021 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
     1081 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct
     1141 cgcggttttg ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct
     1201 tccatgattt taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta
     1261 ggcatggatt caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga
     1321 cctgcttttt ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat
     1381 ttcattaatt cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta
     1441 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
     1501 gatagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
     1561 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
     1621 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
     1681 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
     1741 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct
     1801 catagtaact atatcacgat attacaataa tattatatca cgatatcgta atattacaat
     1861 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1921 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1981 tatccttcaa tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct
     2041 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     2101 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     2161 cttatatttt tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta
     2221 tttttatttc ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa
     2281 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     2341 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     2401 cctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     2461 agacgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg
     2521 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     2581 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     2641 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     2701 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     2761 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2821 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2881 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2941 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     3001 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     3061 ccccattcat cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt
     3121 atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg
     3181 atataaagat tattcatatg ttattcgaaa ttaaaataat cactttttat tactttaaaa
     3241 ttttttatta atgataagaa atatgttttg cgagcagcaa ctagaataat ttaaacatta
     3301 ctaaactgtc tatattgtta ctaataattc ccttcttata gtgcattttc ttagaaaaaa
     3361 acaatattac gatatcatga tattaagata tcgtaatatt atacaattgt gattgatatt
     3421 atatatgttt ttttattttt ttatcatgtt taatcttgaa ggaatcatat tctttttcta
     3481 aagacaggag cttttcataa acatcagaca tgtttaaact agaggccagg cttaaatcat
     3541 aaacgaatga agcaggtaat gattttattc tacgttgtcg ggttatccag cttgcaaccg
     3601 ttccttgtgg gtaggcatat aactcacaaa attgttctac agtgattcct aaattcttga
     3661 taatgtaaac attgatcggg tgcgagtaaa caaatgtatt gatagacatg gtgcgtaagc
     3721 ctactttctt ttattattgc gtttgcaata atagtttaac acacgcatta ttctagtatc
     3781 tactcctatt tgtaaaaaca aaaaaggatt catagagata actctataga atccgtaaaa
     3841 taccttatat atataacggg aagatataaa acctaaaatt atgataacct gctgcagctt
     3901 ctacatagaa agagaagcgc tcaacaccaa cctcatttat caaacgttgg ccataacccc
     3961 aaatctcttc tgatagctga tcaaaggtta tatactttag ctcatattgc tggataatgt
     4021 tcattctaat cgactccatt tattatcctg gtaaaattcg atatgacgta tttcgtcgta
     4081 gtgaattttg tcttttccaa cgtagatccc cagctcatca ctacctaaga tcttgccaat
     4141 gatatcttct ggatagtatc cttcagcgtt tcgttcttct ttttgaatag atactgatct
     4201 attttttatg actgcagcgt tcaatacttc cgcaatctct tcagtcgtca tttgttcttt
     4261 tgctaaattt tgactggttc gttgttcatt gtcggcatca atttcttcag tatgatcaga
     4321 taaataaaac cctgcccact ttaaacgtcc acgatcattg taatcaatca tttttaattc
     4381 ccccatttcc gcccgcatga ccaccaacaa gtgtgcttct agtaatgctt ctagcgcctt
     4441 ctaacattga agaagcatgt acaatcgacg taaaaccata tttttgccga atcttgtcaa
     4501 taattttatc taaattttct tcgtcaattt gcttttgtgg atcactaaat aaatccagct
     4561 gaagcgaatc ggtgtacaca agcttgctat aggttattcc aacatgcctt acttcttgtc
     4621 cttcataata cttatcaaac aagaaaagac aataattctg caattccttt gtattatctg
     4681 tgatagggat cttcatctgg tgtgaaaacc ctggtctcgt ttctaagatc gacgtgccaa
     4741 tgtgtaaatg gacacaacct gttttacagt tatgctgcct aatgcgtatc gctacttgtt
     4801 ccgccatttc ttttaccacc agctcgattt catttcttct agtataatct cgtggtaaca
     4861 cttgtgaatt tccatatgat ttttcctttg tcggttctgg tgggattgct atatctgttc
     4921 gatcgatccc attagcatgg aagtacagct gtaagccaat cacacctagt ctattcttaa
     4981 tcgtataagg attccagtta gcaagatctc taatgctcat aatccccatc tgattgagtc
     5041 tttttttcat tcgagagcca attccccaaa agtccgtcat ttcaggaata ttccatactt
     5101 tttcaggaac gttttcgtac gtccattccg caacaaatcc ttcattatgc tttgcttcgt
     5161 tgtctaaggc tagtttagcc aaaaggggat tatctccgac acctactgta gcaatcaatc
     5221 ctagctcttc tttaatattc tcctggatca tttgagccag cttctttctc cgttgacttc
     5281 gtgttccttc agtcgtaaaa agattcagtg atcgggtcac ttttaagatt gattcatcga
     5341 tcgagtaaat cagtagatct tcatcagcca catatcttct gaaaatatta tttacctgca
     5401 tatttcgctt gatatatagg ttcatacgtg gtggaacaac gtgtagtgtt ttaggaaatg
     5461 gttgtggtaa gtcacgtggt ctactcacat ttgtaatacc ataccgcttt tttgcttcag
     5521 gagaagaagc taatatcaat cctgaaccag tattatcagc tcgactcata acaacaagtt
     5581 ctgttgttaa tggatctaaa tttctttcta tacattcgac acttgcgtaa aagcttttca
     5641 tatcgattaa aaagtaatca tttactggct cttttgaata atccatcata aataacactc
     5701 cattctttca cattatacaa acatatgttc gatattgtaa agaagaaata gctaaataca
     5761 taaaaaaaga agcataggta tgctatcacc ctgcttcttc ttttgatttg tatgatcaat
     5821 ttattctatc actttactaa ttaatttgaa aatctaataa aaactctcac tctaaaagag
     5881 tgagagactg caaattagag tacggacttt tttggagttc cgttctttaa tattaacaaa
     5941 tgaataaaat ccttacaaca tttgagaata agttatcagt agacacttac aaaaaattct
     6001 catactattt cctccatcat tagtttttgt ctatataagg tttaccctaa atagacactg
     6061 gtataacatt gaaaaatatg gcttcctcgt gtatgattag ggtgtatttt aaatagttgc
     6121 agaaagtagg taaaagtttt gagagttgca tacatacgtg tctcatcgat cgatcaaaat
     6181 gaacaacgcc agattgaaga aatgaaaaaa ttcggtgcag agagaatttt catcgagaaa
     6241 caatctggcg caacgattac tcatcgacct gttttccaag aagcattaga ttacgtccga
     6301 gatcaagata tttttattgt ggaagctatt gatcgacttg gaagaaatta tgacgagatc
     6361 atcgcttcag tcaattactt gaagaagaaa aatgtaaagc tgatcattac tagtttaccg
     6421 atcatggctg aagcaattgg caatcctctc ctggataaat tcattaagga tctgatcatc
     6481 caaattttgg ccatgatcgc tgaacaagaa cggactgaat cgaaacgtcg ccaggcacaa
     6541 ggaattaaga tcgcaaaagc aaacggcgtc tacaaagggc ggcccaaatt atacagtgca
     6601 gacgcaaaag atccccaacg tcgccttgtt tacaaaagca tcgttgaaga tcttaaaaat
     6661 ggtgttgcga ttgctaagat tgctaaagac tacaatgtta cgagacaaac cgtgtataga
     6721 ataaaaaaag acagcatggt caatgacaaa tagttgatca tgctgctttt attatcttct
     6781 catctttaat ttcctacata taattactat ctataaaatt aagtcttact cagaacagat
     6841 aaagcataaa attccaacaa accatttttt acttaactaa ataaagagtc tgttaatata
     6901 atcttcactt atttacgtta atcccctact gtatttgttg gttccttatc tatattttct
     6961 acaaaatcct tattatcttg tgatttagct tcgtataatt ctttgtatgt cgacacattg
     7021 gttatactaa ttcctatagc tagttcttga gactctactt ttctctctag ctcctttgac
     7081 aaagtgattt gagatttatt tggttccgtt ggagtcaatt ctatagattc ttcataaaaa
     7141 tctctataca taaagctatc attaatttta taaaaaaaat taaatgtttt tagcactgcc
     7201 atataacatt caaaacattc ttctatagtt ggtttacttc cttgaggaga atgaaccaat
     7261 ttattacgtt tttctatgaa atttttaatt tcctttctta tttcttt
//
LOCUS       DACGLH010000116         7043 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.122, whole genome shotgun sequence.
ACCESSION   DACGLH010000116 DACGLH010000000
VERSION     DACGLH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.122"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..249)
                     /locus_tag="IXT21_002722"
     CDS             complement(<1..249)
                     /locus_tag="IXT21_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870079.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYF"
     gene            complement(386..799)
                     /locus_tag="IXT21_002723"
     CDS             complement(386..799)
                     /locus_tag="IXT21_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ4870080.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1255..1740)
                     /locus_tag="IXT21_002724"
     CDS             complement(1255..1740)
                     /locus_tag="IXT21_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870081.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1879..2094)
                     /locus_tag="IXT21_002725"
     CDS             complement(1879..2094)
                     /locus_tag="IXT21_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ4870082.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2318..3118
                     /locus_tag="IXT21_002726"
     CDS             2318..3118
                     /locus_tag="IXT21_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4870083.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3137..5005
                     /locus_tag="IXT21_002727"
     CDS             3137..5005
                     /locus_tag="IXT21_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4870084.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4998..5489
                     /locus_tag="IXT21_002728"
     CDS             4998..5489
                     /locus_tag="IXT21_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ4870085.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5477..6031
                     /gene="srlA"
                     /locus_tag="IXT21_002729"
     CDS             5477..6031
                     /gene="srlA"
                     /locus_tag="IXT21_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ4870086.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6049..>7043
                     /locus_tag="IXT21_002730"
     CDS             6049..>7043
                     /locus_tag="IXT21_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ4870087.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2474 a         1125 c         1366 g         2078 t
ORIGIN      
        1 gaaatattct aacacaaata tgaaaaagat ggccgaagct aataagccga tttcgtagca
       61 aaacaatgag ataccagagt cgtaataata aaatcctcta atcttatgga taaaatagat
      121 catcggacaa gcagataaga gtaagtattt gttaggcctt ttgaataagt aaaagaaagc
      181 actggtcatt atgataaatg gtatccaaaa caaaagttta cttaagacaa tcaaatgatc
      241 aactcccata ttttttacca agagagatca taacatcttt atcgtaaaaa caaaatattt
      301 ttttgctcat tttgaaaatt taaacatact cgttcttatc caaatatata aaaatcgccc
      361 ctcattcatc gagaggcgat aagttctaat ttaactcaat tttttttgtt tcttttggct
      421 gataagctgt tttaggtaat accaatttca gcacaccgtt tttaaaggtt ccagtgattt
      481 tttcttcatc aacattagga agatagaatg aacgtttgta agagaccgaa tgtctttctt
      541 tccgtacata gttgccatct tctttatctt ctgtatggga ctcatgattg gcggagatcg
      601 tcaaagtatt atctgaatat tcaactgtcg tgtcttcttt gtcacagcca ggcatatcag
      661 cagttagttt gtattctttt tcattttcga ctaaatctac ttttggataa cttgaaacac
      721 ctaaaaaatc attgaatgct ggactaaaca cgtcattgaa atcagggaac atgtcgcgaa
      781 tgctaggtac atttgccatt tttgatcatc ctttcaataa gtatttttta tttatcccta
      841 tttacgtaat aactctattt tgaaaaaaat gcaagtataa tggttagctt tgtgaatatt
      901 tcatctaata cgatacaaag gctatctttc taatttgaaa aagggatcat taaaataata
      961 agaagaaaat atgtcaaaag gtaaaaaagt aaattcttct atccaaatat agaaaaattg
     1021 aatctagaaa accgcccgac aataaatttc attaactcag ttgaccgaaa atgaagtaga
     1081 taccaaaaat ttatcaagag agcaagtaga gaagtgagta tattcataag agaaatagct
     1141 gtaacgaatt attaggttaa tgcaaatgct tatttatact ccgtataact ttctcagaaa
     1201 aaatgatacc aataatgact tgctagtact gtaaatccgt ataatatcat ctaatcaaaa
     1261 tggtgttcca tttgcatctg tcatcgtaat cgatccttcg ggtgacactc gataaaaacc
     1321 aacgcttcca gatcctcctt gggaacggat ttgtttgctg gaagctgtga acaaataatc
     1381 tcgatcaatt ttttgataaa agctaataac aatatcgtca ctttgtaatt cggaacggtg
     1441 ggcaataaga aatagaagtg ctttgtcaga gttttctaat ggaagagaag gtacagtgct
     1501 ttcttgcatt tcagatgttg aagaaatgct aaacgactga atacttgatt ggctatgttg
     1561 cgaatctaca ttaccagtag aagtattagt aatggaagta gtagaagtac ttttagttga
     1621 accaacctca gttgttgaaa tggctgctga agattcttta acagaattct ttgttttata
     1681 tccacaggca gaaaaaagta ttacagttcc caacaagcct gcgtaaatcc ctttatgcat
     1741 gatagctcct cctaaaatag attatcttta ttcagtatat cacttaaaca aagaaaagca
     1801 gatggtttaa aatgaaaaaa ccttcccaat aaagggaagg ttcatgaaga taataacttt
     1861 tttaataatt atagctttct aatatctttc atcaactttt ctagtaatca ttgggaaaaa
     1921 tttcaagcag tagatcgcac aaatacctac gacgatataa atgatcttcg caaaaatagt
     1981 tgttgaacca ccagcaatca tagctactaa atcaagttcg aacaagccta ccaacagcca
     2041 gttcaaccct cccacaatca ataaagttaa tgcaatagca tctaaagttt tcattagaat
     2101 tctcctttct taaatgataa aacaattata tatcaaaaag agaaacattg cttatataac
     2161 gctcaggaat tttagcaatc tatgcaaaaa gatgaaaatt ttttttgctc ttccattagt
     2221 acacattttt tagctgaagc tgtaaatttc atgtcttaat tttttttagt gagcttttat
     2281 actggatttg taagaaaaaa gaaggagagg atcacatatg tcggattggt tggatattgc
     2341 aggaaaagta gtaattgtaa ctggaggttc ctcaggaatt ggaagaagta tcgtcgaaaa
     2401 tttgttgaag cagaatgcac aagtagcgaa ttttgatgta actgaatgcc gtatacagca
     2461 tgaaaaccta ctatctttaa aagtcgatgt gtcttctaaa acggacatag aagaagggat
     2521 atataaagta atgaaacatt tcaagacgat cgatggtcta gtcaataatg caggaataaa
     2581 tattccttct cttctaatag atagaaatca tccaaaaagc aagtatgaat tatcagaaca
     2641 agtttttgat aagatgatcg ctgtcaatca aaaaagtgta tatttgatga gccaagcagt
     2701 tggaagaatc ttggtgcaaa agggatcagg agtgatcgtg aatctttctt ctgaatcagg
     2761 attggaagga tcagaaggac agagctgtta tgctgctaca aaagcagcga tgaacagttt
     2821 tactcgttct tgggcaaaag agttaggaaa gaggaatgtt cgtgtggtag gtgttgcacc
     2881 agggatactg gaagaaaccg gtttgagaac acaagaatat gaagaagctc tttcttatac
     2941 gagaggtata agcgtcgaac agctgagaaa cggttacagt cgaacaagta cgatccctct
     3001 aggaaggagt ggtaagctgc aagaagtggc ggatctagtc tgctattatc tttcagaaag
     3061 atcaagttat atcactggag tcacgactaa tatttcagga ggtaaaacaa gaggataaag
     3121 gatagggggg acgcacgtga cgctagtaaa cagatggtac caattattga aattactagt
     3181 cactcacaag aaagtttcaa tccaggaagt gcaaaaagaa atcaaaacca gtaatcaaac
     3241 cataaaaaaa gatattgggg agttgaatga agaaattgcc ggtattgcaa aaatcatcca
     3301 aaaaaacaaa cattttgaac ttgaaattta tgattttgat ttttttgatg agatcatgca
     3361 aggaaaattg aaaacagcca gtgatttcaa ttcggcatcc aaaagaatcg cttatttact
     3421 aaataggctg atcgaaacag aggatttctt acgtatggat gatttgtctg aagaagtcgg
     3481 tgtcagcaga ggaacagtgg tcaaggatct aaagacatta aaagagatga tcaatgattt
     3541 tgatgtaaag attacaggca caccaaataa aggactaaga ataataggag atgagctaga
     3601 attacgacta cttttatttt attttgtttc tccctacttt tcagagacga aaacagaaca
     3661 gtttctagaa gaaaaacaag caatcaaaca ttttcagaag aagacgaatg ctagtaaaca
     3721 agaaatcacc cttttaacga aagttatcgg tatttctttg aatagaatct ctagtaacta
     3781 tctgttgaag aagcccatca atcgttacag tgggtgtttt gaatatatcc aagaattcaa
     3841 tgaactgatc aatcagttgg aagaaatcta tgaactaact ttgagccaat atgaaaaaga
     3901 ttttatcagt tttcctttga acatcatcaa cacaagtata acgatgataa ggaaagctaa
     3961 ctctgatttg cgcttatatt tcgaccccat gatgatgcgt attcgacgat tagatctcat
     4021 tgatctggat gagtccttct tatatcaaga gatgaaacag cacctctctt tattgataaa
     4081 tcgggtaatt tttcgttatc gattgactga tttattttat ggtgagatag aaaaaaagta
     4141 tcctttttct taccaaatcg ctagcgaatg catacaagca ttagaagcta tcttgcaaca
     4201 taagatctct aaagtagaga tcagctacct aacgttgtat ttggagttag catttcgata
     4261 tcaaaaagag catttttcct ctaaaaaagt agccattgtt catacgacag gaaaaggaac
     4321 agcattaatg atccaaagac aaatcaaaag gatattgggg agggatatac aaataacaca
     4381 tgtttcagaa gaacaatatg aaaaagtaga tctaaacaaa tatttcgctg tttttacaac
     4441 gattccgtta aaaaatgtaa atgaagagac accggtgatc catttgacta atctttttga
     4501 tgactcttgg ttaagggcac aatggcaaaa agcaaaaaaa aagcgattag ccgggtttca
     4561 ttctactgtt tttttctatc aaatatggaa agaaaaaaag acgtatcaag aatatttaaa
     4621 caaattagtc gctcctttga tcagaggcta ttatgtggat gaagaatttg aaaaaaggat
     4681 cttgaaaaga gaatatcagc aaagcacatt tgttaacaaa gaatttgctt ttcctcacac
     4741 aaccaaccag ctatctcaac acattgtttt atcagtaggg ctaataactg atcaaggttt
     4801 agcagaaaca ggaggaaatg gagttatttt tttatttgcc atacctgaag aaatgacggc
     4861 tgaagttgag aatgaattat ttgagctatg cgatatccta tttgaactca taaaaatggg
     4921 aatagatgag tttattgaat tagcaccgac acaagaatca tttttaaaac taatcaaaag
     4981 taaaggagag agacttcatg aatgatatgt atctattcat agttttcgca gcttcagctt
     5041 atgtgctgca agcagttatc agttggaagc aactaaagca tttcaatcaa acatatagcg
     5101 aactgcgaag aaaaggaaaa gtagcgattg gtaaacgacc tgggaaaatc cgttcaggca
     5161 cgattgttat gctagctata gacgatcatg atcaagtttt agaagtcaga aaaatgcaag
     5221 gggttacatc tttggcaaaa tttcattcat tacctgtcta tgcgggtgaa gatatccatt
     5281 acatcgatcg ttaccatccc tcagttcgag cggaagatcg tttgacacaa gcagctttag
     5341 aaaatgccag agaagtatac ttacgaatag aggcaggaaa tgaaaaaagt gaacaacctt
     5401 cattgccttt tattgctatg ctcaataatt ttcggatatg gaaaagtacg atcaaagaaa
     5461 aaaggagtga caaacgatgg atatcataac aaaatttgct gagggattta tgaggctgtt
     5521 ccaaacaggt gcagaaacat tcataagctg gatgacagga atcgtaccag ttgtcttgat
     5581 gctgatggtt gcaatgaata cgctgattgc ttttttaggt gaagaccgag taacgaaagt
     5641 agccaaatta tcagcgaaaa atccgcttac tcgttacctt gtgttaccgt tcttatctgc
     5701 ttttatgttg ggaaatccta tgtcatttac aatggctcgt ttccttccag aatactacaa
     5761 accaagctat tatgctgctc aagctcaatt ttgtcatacg agcaatggtg tattccccca
     5821 tattaatcct ggagaattgt ttgtatggat ggggattgct caagggatcg aaaccttagg
     5881 gctaaattcg atggagctgg ctatccgata cttgcttgtg ggactgctga tgaactttat
     5941 tggtggttgg ataactgatt ttactactgg atttgtatgc agacagcaag gaatcgttct
     6001 tagcaaaaaa gtagagcttt cagttgatta gagaaaggag agttttaaat gtatggtgat
     6061 attaagatcg taagaggaaa cggcgggtat ggaggtcctt tgattattag gccgacaaaa
     6121 gataaacaca agttcatcta tgtcacaggt ggaggtgaga ggccaatgat cgtagataaa
     6181 atagaagaac tgacaggtat ggagtctatc aatgggttca aaacatcaat acctgatagt
     6241 gaaatcgcgc ttgcaatcgt tgactgtgga gggactctta gatgtggaat ttatcctaaa
     6301 aaagggatac caaccatcaa tatcgttccc acaggaaaaa gtggcccatt agcacaatat
     6361 atcactgaag atatttatgt ttcagcagta gggttagaac aaatacagcc actaatggaa
     6421 actgatgata acaaagaaac agtaaatact gcatctccag ataaaaagta caaatatgat
     6481 acttctaaaa agataaccga gcaaaaagca gagcagcaaa actggatagc aaaaattgga
     6541 atgggcgcgg gaaaagtagt tgctacattc aatcaatcag caagagaagc agtagatacg
     6601 atgatccgga cagtgatccc ttttatggct ttcgtttccc ttttgattgg tgtgatacaa
     6661 ggttctggga tcggtaatgt atttgctaac attatggcac ctttagcagg aaatgcagtt
     6721 ggattaacaa tcattggctt tatttgttca ctgccatttt tatctccttt gcttggtcca
     6781 ggggcagtta ttagccaagt gattggaaca ttgatcggtg tagagattgg gaaaggaaat
     6841 attcctcccc aattagcatt accggcacta tttgctatca atactcaaaa tgcatgtgat
     6901 tttattccag tcggtctagg tttggaagaa gcaaaagctg agacagtaga agtaggtgta
     6961 ccatcagttc tttattcaag atttcttaac ggcatcccac gagttatcat tgcttgggta
     7021 gcaagttttg gattatatag ctg
//
LOCUS       DACGLH010000117         7018 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.123, whole genome shotgun sequence.
ACCESSION   DACGLH010000117 DACGLH010000000
VERSION     DACGLH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.123"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            2..74
                     /locus_tag="IXT21_002731"
     tRNA            2..74
                     /locus_tag="IXT21_002731"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IXT21_002732"
     tRNA            78..150
                     /locus_tag="IXT21_002732"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IXT21_002733"
     tRNA            155..225
                     /locus_tag="IXT21_002733"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IXT21_002734"
     tRNA            238..311
                     /locus_tag="IXT21_002734"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IXT21_002735"
     tRNA            323..394
                     /locus_tag="IXT21_002735"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IXT21_002736"
     CDS             660..1853
                     /locus_tag="IXT21_002736"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ4870088.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IXT21_002737"
     CDS             1983..3455
                     /locus_tag="IXT21_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4870089.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IXT21_002738"
     CDS             3538..4326
                     /locus_tag="IXT21_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ4870090.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IXT21_002739"
     CDS             complement(4393..5061)
                     /locus_tag="IXT21_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ4870091.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IXT21_002740"
     CDS             5176..6888
                     /locus_tag="IXT21_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4870092.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
BASE COUNT         2066 a         1207 c         1566 g         2179 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacat
//
LOCUS       DACGLH010000118         6581 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.124, whole genome shotgun sequence.
ACCESSION   DACGLH010000118 DACGLH010000000
VERSION     DACGLH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.124"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            107..928
                     /locus_tag="IXT21_002741"
     CDS             107..928
                     /locus_tag="IXT21_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ4870093.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYLGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            948..1508
                     /locus_tag="IXT21_002742"
     CDS             948..1508
                     /locus_tag="IXT21_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAQ4870094.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNKTHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2416..3183
                     /locus_tag="IXT21_002743"
     CDS             2416..3183
                     /locus_tag="IXT21_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ4870095.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3209..3754
                     /locus_tag="IXT21_002744"
     CDS             3209..3754
                     /locus_tag="IXT21_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870096.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3755..4321
                     /locus_tag="IXT21_002745"
     CDS             3755..4321
                     /locus_tag="IXT21_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870097.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4336..4584
                     /locus_tag="IXT21_002746"
     CDS             4336..4584
                     /locus_tag="IXT21_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870098.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4603..4842
                     /locus_tag="IXT21_002747"
     CDS             4603..4842
                     /locus_tag="IXT21_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870099.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4859..5443
                     /locus_tag="IXT21_002748"
     CDS             4859..5443
                     /locus_tag="IXT21_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870100.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5550..5831
                     /locus_tag="IXT21_002749"
     CDS             5550..5831
                     /locus_tag="IXT21_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ4870101.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDKPVDVLDTDEKLEEWFNEA"
     gene            5821..6162
                     /locus_tag="IXT21_002750"
     CDS             5821..6162
                     /locus_tag="IXT21_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4870102.1"
                     /translation="MKPNEIVTVYAAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2490 a          845 c         1342 g         1904 t
ORIGIN      
        1 aatcaaattt tttataaaag taatatataa aataaattgc ttgaagtatt atatttagat
       61 atcataattt atggaagaaa aattaattaa aaaggatgag attataatgg ggttactggt
      121 tccgagagct aatacttata ctataccttt aacgaaacgc caattaattg ctaaaagata
      181 tcaaaggatt actagagcca tcaatagaga gttttggaat tctgaaagtg atactgctca
      241 cagtttatac gtaggttctt atgggagagg aacggcaatt agtactagtg atatagatat
      301 aatagttgaa ttaccgatgg cagaatttga cagattcaaa aattatttat caaatggacc
      361 gtctaagtta ttacaagtta taaaaaacgc atttcaggaa atactaccaa atagtgatat
      421 tagagctgat ggacaagttg ttaaaataaa tttccatgat ggaatcaaat ttgaaatagt
      481 tccagctttt aatgaaaaag attatttggg tgagtcaaaa gggtttattt atccagattc
      541 aaacatggga gggaactgga aagctacaaa tcccaaaaaa gagcaagaag ccatgaaatt
      601 aaagaataca aaaagtaata atttattata tgctacgtgt aaacattttc gacatgtacg
      661 agatacagaa tttacgagtt atcatctttc aggaatagta attgacagtt tcgtatatga
      721 ggctatggga aattggaaat tcgttgaaaa taacagtggg ggtcaaaaca ttagttctgt
      781 atcttatgag acagctttat tagaatacta taactcacat aaagttatgg gcggtttaaa
      841 tttatactct cctggaagca atcaatttgt taattctgat agtagtatca tatgtttaga
      901 aaaagtatta aaaaaaatag ctttataaag ttttatggag gaacaacatg ggagacgaaa
      961 aaaagcgctt ggaagttcaa ttacgtgaag catatggacg agtaacgtat acttacacca
     1021 ctcatttaaa gcaagccgat aatattattt ctgacaataa aattttaagg tgtcttcaaa
     1081 ttggtttgtc agcccttact actggagggg tacttagtac cattattact gataatagtg
     1141 agttaacctt tgttgctgga ttatgttcaa caatttctct tggattaaac ttatacttta
     1201 agaattttga tcttgctgaa gaagctaaaa atcatcaaat tgttgccgat aaattatggt
     1261 tgatcagaga agaatacgta agcttactaa cagatttaaa tcgtttaagt gttgaagata
     1321 ttgtgaaaaa aagagatcaa ttaatgaaca aaactcatga agcatataaa aatgccccta
     1381 aaacaaatag taaaagctat gaatcagctc agaaagcatt aaaatctgaa gaagagcaat
     1441 tttttacaga agaggaattg gataagatgt taccagagca tcttagaaaa aagagttcta
     1501 aatattgaac tcttttttta tgcttaaaat tttcttgtaa tttttctatt caatcaataa
     1561 ataatctata tttaagaaga aatatgatga tagaaacatc tcctaaagcc accgcaagcc
     1621 gtcctagcca ttgcccagtc cgacatcgct agattagtgc atctctccaa aaaagttttc
     1681 taaagccacc gcaagccgtc ctagccattg cctagtccga catcgctaga ttagtgcatc
     1741 tctccataaa agttttctaa agccaccgca agccgttcta agcatagctt agcccgacat
     1801 tgctagatat gctcatctca ccatgaacgt ttcctaaagc caccgcaaac cgtcctagcc
     1861 atcgcctagt gcctagtccg attttgcttg atcggttcca tttctgttta tttagtgggt
     1921 caacaccgta atcttaaata gccatcgcct atttaagatt actaggtctg ctttgaaggt
     1981 aaaaagtctt tcattcttca agacttttag gctttcaagc tacgcctgaa agcattgaaa
     2041 ttcatcgctc tttatttggt tttgccattt ataattcctt acgcccatta tctttttcgc
     2101 ctgtcatacc ttgtcagaag tgtttagagc ttatctcatc tttggattaa tacaagtggc
     2161 gtaaattttt ttctaaacgc aagcgttacg aaaataattt acttaaacac ttgtattaat
     2221 ccggcagata agaacgcatt ctaatcactc ccgacaaggc aagacaggcg aaaaagataa
     2281 ataaatgaac gaaaggaaga aacataaaat gcaaaaccaa atagcgatga agaaaaaacc
     2341 acctccctct aaaacaatcc aaacaaaaaa gaattttgaa aaaccaaaaa tcaaaaacag
     2401 aaagaagcgt tcaacatgag agatagaatt ttagaagtta atcaagaaat ggtaaacaag
     2461 ttaacaaata tcagtgggaa ctatgaaaag tacgatggta ctttacttga taattatatt
     2521 tttcacgata cacaaaatat cagaattggc agaagtaaac cgagagcgtt cattctcatc
     2581 aaagagaaat atttgaatga atggaatagc acctatgaaa tggttatgac tgataacttt
     2641 gaaaaggtat tggatttcag aaatgctttg ttgggttctg atatgggaaa tagcagtctt
     2701 gaatctattg aagttgaaaa caagaccgag aatgaaggtg aaggattgat taagaatgtc
     2761 ctgaaaatcg aacaaaaata tatcagtgaa gatagaagtt tttatagagt cacaagctcg
     2821 gttggtctag caaactttgc tactaactta attggaaaca atgctagtga atctttgcta
     2881 gtgatgtgtc tgaatacaaa gaatgaggtc attgcttata gcgaagtttt caaaggtgga
     2941 attaatcaat cagtagcgat accaagagag atttttcaac tagctttgct aaacaattca
     3001 gcaaaaatcg cagtatcaca taatcatccg agcggatatg aatttccaag tgaaaatgac
     3061 aaaagtttta ctacgaattt gaaaaacgca ggaaaaacaa ttggtattga attgttagac
     3121 catatcatcg taacaagtaa aaacacaagc tattactcat ttcaagaaaa tggttcttta
     3181 taaaaattag agaaaagagg aaaacaagat gggcgaaaga acacaagcgt taattacagt
     3241 caaatatggt aacagcaaaa cgaaagtaag cgtgtcaatt cattatcagt ggggatatgg
     3301 tcgattcatg ttgatggacg ttttgaatca aggaatatat ttaatcaata aagcatatga
     3361 cttacgagat gaatcaagag aagaattagc aaaaggaatc tttgatagaa gtacaggagt
     3421 tatcaatttt gtcacctatg aatctggagt gagtgcttat gatgaaacag ggtttggtgg
     3481 gcgctatgaa ccttatcaag aaacgattga agatgtgatg aagttttcag ataacaatga
     3541 tggctatgct catttagaga tggtgttcga tgaaaatgga tttagtctag atgataaaac
     3601 cagattgcga ttgtttgatt ttgagggtca acagatagga ctaaacaagt tttgtgaaag
     3661 cggaaaagag tttgctacga aagattttat taagggatac agattattgc tagaaaatta
     3721 tggaataaac ctagaagaag agagcgtgaa ataagtgttt gaaaaaagaa aagaccagtt
     3781 ccctaattta gaagcgaaaa gtcaaaaagt gattgatggt gaaatgattg aattattcca
     3841 aaaaagttat ggaaccatag aaactaccgc aagttgcaaa tactactttc aaggaaagag
     3901 aaacacgttt aaaggctttg ccgattctga tgaagaattg gcaaagaaaa tcattgattg
     3961 gcttgtcagc agtgaacacg tagcaagaga agataccgtt agaacaacga ttgagggtga
     4021 agtcctaaca ggtactagat gtgtttgttg taatcatgaa tttgtagaag atgaaggttg
     4081 ctaccgaatc aatgaatatg gtgattttat ttgccagagt tgccacgaga tagaatttcc
     4141 aaccgaaaga agttggtttg aatacatgct acaagattat gagatggaaa cagaaaatgg
     4201 tgaagtagtt ttttgtacca aagaggaatt gagtaagaga agcgatgatg aaattgaaga
     4261 ttggtgtgtg tggttagtag ggacagatga ttgcgttgca tattacacaa cagcagaata
     4321 ggagtgtgaa tcagcatgga aggaatggat tttatagatc atgaagattt gattgatttt
     4381 ggttatacat ggaaaggaat ggtaggtatt agcagaagtc ttgctaatgc cttttacgaa
     4441 agaaattatg cagtctatgt cctttatgat gacaatacag aaagccttgt tgatgaagag
     4501 tataaattgg atttagagaa tgttttatat gggatagaaa aagaagattt agcaaaatat
     4561 attttttcat ggttgggtca ataaacgaaa gagggtagca acatgacaaa attcaaaagt
     4621 aaaggtatgc aatggtgttc ttattattat gttgggaatg atgaagagct tttgagaaaa
     4681 gccaaacaaa aaggttatcc agataagatg ttaaaacaga ttggctatta catgccagaa
     4741 gagagaatag aaaaagaatc ttgtaaggtt ctttttgata gtgaacagct attagttttg
     4801 ttaactcaaa ataaattgat ttgtcaattt gaaaataatt aatatggggt gaagaaccat
     4861 gaatgagaat aagatggtat taagtgaata cgcaagattg agaaccatta attggttgaa
     4921 tggtatgcaa agtagtggcg cttttgatgg atttgatgag gagaatatgg actttgaaag
     4981 tttatcgaat gaggatatta gatattattt tatcgaatgg tattttacag aaccagatga
     5041 agaggtggaa gatgaaccag aattaatttt agaacgtcaa agaattttat tcagtgaaca
     5101 agatagtaag aggttgtatg gtttatatga cttacaactg aaaggattaa aagcgcagta
     5161 tgttgctgat agttacgatg atatggtaga acagggggca gatagggttg tcacttattc
     5221 agaagatgat gaagaggaat gggaaaacta ttcagatgat gagatatgct ttaatgatgg
     5281 gtatatgcct attgaaatca ccgaagaaga atatgataaa gtgtcggata gcgatgagat
     5341 tggtatgtgt acggttgtaa gtggattgga tatagatgtc caaagagtag aggttgcata
     5401 ccagaagatg aaagaacgta taaaaagtaa agtagaatct tgatttgatt ggattgtttg
     5461 caaagtagct aattaagagt ataatataat cataaatgta gttaatggat ataataatga
     5521 gtatattata ctcaaaagag gtgaacgaaa tgaaggtaaa aaatccaaaa gaaaaaagca
     5581 gaattcaagt cggaatagac aaggatttaa aagaaaacgc agaaatgata ttggaagagc
     5641 tagggttaaa tccaacaaca gcaatcacaa tcctttataa gcaagtggtc gctagaggtg
     5701 agtttccagt agagataaag ctaagcgaag aggaaaagca aggaatcaga ttgcaacagc
     5761 taactatgga taagccagtt gatgttctag atacagatga aaagttagaa gagtggttca
     5821 atgaagccta atgagatagt aacggtctat gctgcattta cagataaaaa tggtggtaaa
     5881 agaagaccta ttttggtggt tagtgataaa gaagatagag tagaattttt tggaatcaca
     5941 agtcaatatg agaataaatc agaccaaata aagagagtgt attttcctat tgcagaatgg
     6001 gaaaaagtag gtctaaagaa gcaatcatgg attgacgtag ggtctttaaa ggctattccc
     6061 aaaagccgag agaacatatc atttaaaaag gttggtatgc tatcagctat tgatacgaaa
     6121 aagctaaata ggtttatcga gagattacaa agaaaagtct aacaaggctt ttcttttttt
     6181 gtttatatgt atattaagat atacgtttat ataaacgtat ttatgtatat taaaatacac
     6241 gtaaatatat acgtgattat aaaaattaag aaaatattaa gtattgttaa atagtgataa
     6301 ttagtaatat acgaattata agatacattt ttatatttaa tgtatgtaaa taaataaaat
     6361 gactgacgga tattgaatag taataaataa caataattca gacggataca gtgagtgaag
     6421 tagcttgcta cgaacgaaca ggcagaatga aacggtagtt tcattagagc aagtcgcttg
     6481 cgacgtagcg ttaccactga tgtaactggc aagttacata gagagagttt gcttgcaaac
     6541 gaacgttacc cacttgaata gaaaacaaaa ataacatatt a
//
LOCUS       DACGLH010000119         6569 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.125, whole genome shotgun sequence.
ACCESSION   DACGLH010000119 DACGLH010000000
VERSION     DACGLH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.125"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            127..579
                     /locus_tag="IXT21_002751"
     CDS             127..579
                     /locus_tag="IXT21_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870103.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IXT21_002752"
     CDS             593..1282
                     /locus_tag="IXT21_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870104.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IXT21_002753"
     CDS             1407..3758
                     /locus_tag="IXT21_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ4870105.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IXT21_002754"
     CDS             3838..4233
                     /locus_tag="IXT21_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870106.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IXT21_002755"
     CDS             4558..5175
                     /locus_tag="IXT21_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4870107.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IXT21_002756"
     CDS             5216..5515
                     /locus_tag="IXT21_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870108.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IXT21_002757"
     CDS             5549..5716
                     /locus_tag="IXT21_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4870109.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="IXT21_002758"
     CDS             complement(5761..6366)
                     /locus_tag="IXT21_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4870110.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2109 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaatttt
//
LOCUS       DACGLH010000120         6447 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.126, whole genome shotgun sequence.
ACCESSION   DACGLH010000120 DACGLH010000000
VERSION     DACGLH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.126"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            116..865
                     /locus_tag="IXT21_002759"
     CDS             116..865
                     /locus_tag="IXT21_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4870111.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            877..1146
                     /locus_tag="IXT21_002760"
     CDS             877..1146
                     /locus_tag="IXT21_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870112.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1308..1610
                     /locus_tag="IXT21_002761"
     CDS             1308..1610
                     /locus_tag="IXT21_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870113.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1607..1768
                     /locus_tag="IXT21_002762"
     CDS             1607..1768
                     /locus_tag="IXT21_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ4870114.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1765..2070
                     /locus_tag="IXT21_002763"
     CDS             1765..2070
                     /locus_tag="IXT21_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870115.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2070..2426
                     /locus_tag="IXT21_002764"
     CDS             2070..2426
                     /locus_tag="IXT21_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ4870116.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2386..2631
                     /locus_tag="IXT21_002765"
     CDS             2386..2631
                     /locus_tag="IXT21_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870117.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2628..3047
                     /locus_tag="IXT21_002766"
     CDS             2628..3047
                     /locus_tag="IXT21_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ4870118.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3044..3601
                     /locus_tag="IXT21_002767"
     CDS             3044..3601
                     /locus_tag="IXT21_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ4870119.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3598..3894
                     /locus_tag="IXT21_002768"
     CDS             3598..3894
                     /locus_tag="IXT21_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870120.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3971..4384
                     /locus_tag="IXT21_002769"
     CDS             3971..4384
                     /locus_tag="IXT21_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ4870121.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(4842..5117)
                     /locus_tag="IXT21_002770"
     CDS             complement(4842..5117)
                     /locus_tag="IXT21_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870122.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            5571..5777
                     /locus_tag="IXT21_002771"
     CDS             5571..5777
                     /locus_tag="IXT21_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870123.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            5973..6140
                     /locus_tag="IXT21_002772"
     CDS             5973..6140
                     /locus_tag="IXT21_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ4870124.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            6166..>6447
                     /locus_tag="IXT21_002773"
     CDS             6166..>6447
                     /locus_tag="IXT21_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ4870125.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTA"
BASE COUNT         2346 a          991 c         1420 g         1690 t
ORIGIN      
        1 tccatcacac agagaagcac aaaataggtg tgattaattt caaaaataaa tatcaaatca
       61 aagggatcaa attaaaccag aaaacaatca aaaagttaaa tattggaggg taaaaatgac
      121 agaacatcga agttattatg cgattatacc agccaacgta aggtacgaca aaagacttaa
      181 accaaatact aagttgttat acggagagat aacggccttg tgtaatgaaa gaggcttttg
      241 ttgggcaggc aatgagtact ttgcagattt atatggtgtg aataaagaga ccatatcgcg
      301 atgggtaagt gatttgatta agtttggata cttgaatcgg gaaatcattt acaaagaggg
      361 taccaatcaa ataatcaata ggtacctacg aattaatcaa taccctattg acgaaaaacg
      421 caatacccct attgacgaaa aagtcaaaga taataataca tctattaata atacatttaa
      481 taatacaaaa gaatatataa gagagttacc gccttcgaaa aaatcgaagg ctaagcccgt
      541 ccgtcataaa tacggagagt ataaaaatgt tcttttgtca gatgagcaga tggagaaact
      601 caaaacagaa ttccctaatg attaccaaga gcgaatcgaa cgactgtcag agtattgtga
      661 atcatctggt aagacttata aaaactattt ggcaactatt cgaagttggg caaggaaaga
      721 aaaaagtgaa cctaagaacg caagcagtgg atacaagcgc acaggaagac gagagaagct
      781 tcctgaatgg gcaatcgacc aagaagccta tctcaagaaa aaagcgctag aacgagctaa
      841 tagacaatca aaagcaccat tctaagaggt ggaaaaatga agatcgatta tctagaacta
      901 attaatgaaa tagcgaatta taaaaagggc gaggaattag acgtcctgag agacgtatat
      961 gatcaactcg aagaagctgg aattgaagga attaagaatg atcgttcgag ttggagtaag
     1021 ctcagatact atttcgcact ttatatcgat acaacacaat taagaaattt agcatatacc
     1081 aaattactat ttgttgattg tgttaaaggc ttgcaaaaac atcttaatga acttgagcag
     1141 gtgtaatcag atggacctaa agacatttac agcacagatt gaactaatgc atcaagaagc
     1201 tttaagacaa agtgtatcgt acgaagacaa gtggctcaac acgttccatg gcggacgtga
     1261 gagcgcactt gatcaagtac tcaaattact gaaaggagaa tgtcgggatg gataagaaag
     1321 cggcaatgca gcgaattatc gaattgactt attcagaaga ttggcaaaat gacaaagaag
     1381 ctgcttcaga agtgatgagg cttggaagag cgatgtgggc agacaagagc aacaagccaa
     1441 gaccacgaaa aatcgcaatt tggcacggtg acaaacttct agtgataggg acagctgaac
     1501 agttagcaag tctcacaggc ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct
     1561 acacagacgc taagaagaga acgtttagat acgtggagga atcatcatga caacagaaga
     1621 agtgattcaa atgcgtattc gaagccttca gcgtgagatt gacgatctgg aacgaacaaa
     1681 ggcagtgatg gtcaatgaaa cggcgagaaa ggcaatcgat ttgcacatag agaatttaag
     1741 aagggaaatc catcgattgg aggaatgagc gtggataaga aagcagcaat gaaaagaatt
     1801 gctgaattaa ccaagtcaga atcttggcaa gaagacaaag aaatagttgc agaagtccaa
     1861 aagctcggta aatcaatgtg gactgaaaag cccaaacgga gaacgccgag aaaaattgca
     1921 atctggcatg atgaccgaat tttagtaaca ggtactgctg aacagttatc tgaaattact
     1981 ggattaagca aaaacattat ctgggataga gctaggagct tatggattga ttcaaaagga
     2041 cgacagttta ggtatgtgga ggagagataa tggatctcat tacacaatac agtgacatca
     2101 tcctcaagaa aatcatgatg aagattcaga aagataaaaa atcaaaagaa cgagctgaat
     2161 tagttaagtt agaaatggct gaaacaggag caggagtgcg aagtagcagg cattggaaag
     2221 cagcagcaaa cattgaattt tattacaacg aaattcaaaa agggttcgat cagatgcgtg
     2281 agctggatcg gcaaacaaat tggagcaaga aacttcatca agatcgtttc aaatttgtag
     2341 agaagtataa agaaatatta gaagagtatt tgaggaggac agcaaatgat aaaaaaactc
     2401 gttcaattca gcatggattt atatgatatc gaatcaggag caacactatc tgtggaatcg
     2461 gaccatctaa tcataaattt tggtggaaag cgccagatta ttttgtgggt agttgatgat
     2521 gtactgtttc cagaaattgt tcatgatttc gaagaatcaa aagcggttga gtttgaaata
     2581 gtgaaaaaag taatggaatt gattgaaaaa tacgaggagg acagcgaatg ataccgaagt
     2641 ttagagcgtg ggataaacga aagaacgtaa tgagagatgt agccgtcttg cattttacta
     2701 aaaacggcaa aacaaacttt attgaatatt ggataaatcc taccgaattg aaatcatatc
     2761 acgtgcgaaa catcgacctc atgcaatcca caggaatgaa agataagaat ggtgtggata
     2821 tatttgaagg ggatatagta ttagtcagcg tgcgaaatgg cttcgattac ttagataata
     2881 aagtctgtat tgtcaaaaat tcaatagatt attccggatt agtttgtgcc actgttgatg
     2941 aagacttaga gtatcaaatt tttaacacag agctgtttga agaatacacg tacgaagtca
     3001 ccggaaatat atacgagaat agcgagttat tggaggaaca gcgatgaata aacaggaagc
     3061 aatcaaaaag ttagaaagta ttaaagcgat aggaaatgat gcaatagctg cttgctataa
     3121 cgagagtata aattcaggta ttacgttaat gaaaaaaata gacgaaccgc agaaaccagt
     3181 tattccacaa ctcgtggccg gttggcttga gaaatctacg gaccctttta caaaagctga
     3241 aaaaatagcg tatttaatca aatctaaaga tggtgattca tattatttct gtgattggtt
     3301 tgtacgagat ggcatagtga cgcaagagca aggagaagaa ttactcgctt gggcaacaag
     3361 acaatcatat gaaacactat tgagcctata caacggctac gaggttgaga aagagccgtt
     3421 atgggcaata aagaatgccg atggaaacta tcttactaaa tgtgctttat ggggaaaaga
     3481 tggagtaaat tatagttttg aatgcaatcc atctcatcga ttgcttttca ctgataaagc
     3541 aacagcggat gctgcagcat tgttggtgaa tggaacagtg gaagaggtgg tagaaagatg
     3601 aaactaaaag acggatttta cgctagcagt cacggtatcg gcggtttaat gctagatatg
     3661 ccgacaaaga accctaaaac acgcaagaaa tcaaaattca aagtcggtga catggtccgc
     3721 tgtgaagcag aagagttcgt ttatccgttc agaggatacg ttaaaaagat actgtcaaac
     3781 tcagcaatca ttcgtattga aaatacaatg aagtgtgatc gttcgactgc aaaacacaag
     3841 cattatttag ctgtagcgag attagttgac atggaagtaa tcaagagcaa ataaaaaaag
     3901 ccggatcgct ccgactgatt caataaatcc aacacattta ttatatcaca taaaggagcg
     3961 gtttgacttg atgcaattgt tacgagaggt agatttcaaa cagacaagat gtaatgcgag
     4021 agatgtgctg aagaactttc ggcgtttgga gcggatggca ggtcgctctt tgatagatat
     4081 taagtcgccg attataacgg atatgccgaa ggcaccgaag catggcaata aggcagaaga
     4141 cgcgattatt cagatgatgg atatagaaac ggagagagat gcgattctag cggctttgat
     4201 ggctcttagt ctgattagtc gtcagatact ctactacagc ttctgtgtgc cagatagctt
     4261 ctcaaactac agaattagcc gtgaagtggg ttattcagaa agaagtatac aacggatgaa
     4321 gtcggaagct ctaatagagt ttgcagaagc atataaacac ggaaaaataa ttgcttataa
     4381 ataatttggc ggttttttgg cggaatgatg gcggttttta gacatttacc agtgttatta
     4441 tggtagtgtc gaaagattag gaaacaggat cgacaaaata aacgaaggga ggcaatctcc
     4501 ctcatcgttt taaacttctt cttagataga cagcaactaa ttatgaagaa aaggatgtga
     4561 attcaactcc tcctaaattc ttcatcggtt gctgtttatt ttgattagac agcagcactc
     4621 acaaaactaa acatgttctt gtcgtctttc aactgctgct gtctattaat ttatgtattg
     4681 gagggaagaa aaatgaaatt atcagtagaa ggcactccag aagaaataca agaattgctc
     4741 caagctattg gtagtagcaa ggagcaaaag gtaagtgttt ctaattcaga tattgacggt
     4801 atttgcaatt gtttaaacag agaaaaaata tctaatcctt actaagagaa aatatgttcg
     4861 ttaatgaatt tccaattttc ctctgtagct ctaccttgat aatttttttg cagttcacag
     4921 atgaataagg aatcgttatt atcaagtact gtatttaatt tttcacacat ttgatttgga
     4981 ttcaaattag ttttaactaa ccaaaaagat ttttgtagct ttatatatgc cccaaattct
     5041 tttattattt caaatacttt atcatacttt tgccctgggt tattaagatc ataactaatc
     5101 atgtagggtt tatacatgtt gtcttgcaca tccttgtttt atatttcagc ggaccactcg
     5161 ctgataaata aaattatacg cttagtattt attttcacaa tattaattta tcgtaataac
     5221 ttttgagtaa atagaacaaa aaacctgcac tagtttccgc tagtacaggc agtgactata
     5281 tcggttgata atctagcata tattaaaaat aattgcaaga aaagaaattt atagtggttt
     5341 ataccaaatt atcaaagcaa tgttattttg ttgctgtcta ttgtttttta attattcaca
     5401 cgataactaa aggtgggtga agagaaatga ttccattaat aatttcaatt tttgcgctct
     5461 gtcttaatgt ctatatgatt ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa
     5521 gaataatttt atagtgtcac tgtggcggaa agggtagacg ctaagcatgt gtgctaggtc
     5581 aatgcttcgg caaccatgca aggttcgatt ccttgccagc gacattaaat gcctatgacg
     5641 gttacgacta ccgaaaaaag atcgttaaga agctatacgg tgctacgtac ggcaatgtag
     5701 taagtgtgct atctgtacac caccaagctt cggtcactgt ggcggaagta gaagacgcag
     5761 cggtaaatgg cgagtagcct cgtgagagcc tggtaagttc tcgtgagtgg tgcaatccca
     5821 ctccagcgac tttaagcaac cgaggcatcg gcggtttaaa aatatagggg tgcgcaattt
     5881 cgtacgcgtt ttgtgcatcg tgcaagttac tattacatat tagatcactc tttgagtggt
     5941 ctttttattt ttgcacaaag gaggaaacaa caatgtatag accacaatac ttagaacaga
     6001 agtatgaagt aatcactgtt cataaaggta atggcgaaaa agtatatgag tatagaagac
     6061 caataaagag cgatacatat aaacgaaagg aaaacaatga agttattcca ttgtatggca
     6121 aaagaatagc taagcattaa ataagattgc gaaaggagac ggaacatgac cgaggaattc
     6181 tatagatggc tattacagtt gacaagagaa gatcgtttgg ttaagttcta tcagtctcct
     6241 aaatggcgca ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt
     6301 agaagactag gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat
     6361 agacctgact tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat
     6421 gaagttcatg gcagatatct tacagca
//
LOCUS       DACGLH010000121         6286 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.127, whole genome shotgun sequence.
ACCESSION   DACGLH010000121 DACGLH010000000
VERSION     DACGLH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.127"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..177
                     /locus_tag="IXT21_002774"
     CDS             <1..177
                     /locus_tag="IXT21_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870126.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IXT21_002775"
     CDS             190..690
                     /locus_tag="IXT21_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4870127.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IXT21_002776"
     CDS             787..2634
                     /locus_tag="IXT21_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ4870128.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IXT21_002777"
     CDS             2637..3533
                     /locus_tag="IXT21_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4870129.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IXT21_002778"
     CDS             3583..3972
                     /locus_tag="IXT21_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4870130.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6286
                     /locus_tag="IXT21_002779"
     CDS             3959..>6286
                     /locus_tag="IXT21_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4870131.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1347 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaac
//
LOCUS       DACGLH010000122         6173 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.128, whole genome shotgun sequence.
ACCESSION   DACGLH010000122 DACGLH010000000
VERSION     DACGLH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.128"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            725..811
                     /locus_tag="IXT21_002780"
     CDS             725..811
                     /locus_tag="IXT21_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ4870132.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IXT21_002781"
     CDS             complement(932..1621)
                     /locus_tag="IXT21_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870133.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILSGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IXT21_002782"
     CDS             complement(1866..2780)
                     /locus_tag="IXT21_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ4870134.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IXT21_002783"
     CDS             complement(2762..3142)
                     /locus_tag="IXT21_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ4870135.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IXT21_002784"
     CDS             complement(3592..3879)
                     /locus_tag="IXT21_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4870136.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IXT21_002785"
     CDS             complement(3899..4123)
                     /locus_tag="IXT21_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ4870137.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IXT21_002786"
     CDS             complement(4763..5296)
                     /locus_tag="IXT21_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ4870138.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IXT21_002787"
     CDS             complement(5317..6159)
                     /locus_tag="IXT21_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ4870139.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1632 a         1152 c         1043 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctgataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACGLH010000123         6028 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.129, whole genome shotgun sequence.
ACCESSION   DACGLH010000123 DACGLH010000000
VERSION     DACGLH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.129"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(25..1497)
                     /locus_tag="IXT21_002788"
     CDS             complement(25..1497)
                     /locus_tag="IXT21_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ4870140.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFIY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVMEGKVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQEKIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            complement(1511..2359)
                     /locus_tag="IXT21_002789"
     CDS             complement(1511..2359)
                     /locus_tag="IXT21_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4870141.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LTSFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            complement(2356..3222)
                     /locus_tag="IXT21_002790"
     CDS             complement(2356..3222)
                     /locus_tag="IXT21_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4870142.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            complement(3265..4578)
                     /locus_tag="IXT21_002791"
     CDS             complement(3265..4578)
                     /locus_tag="IXT21_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4870143.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMESGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDGSA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQVIDTSLK"
     gene            complement(4720..5691)
                     /locus_tag="IXT21_002792"
     CDS             complement(4720..5691)
                     /locus_tag="IXT21_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4870144.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGFSPYPNETNNRKAGFYNICEKNKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            complement(5817..6028)
                     /locus_tag="IXT21_002793"
                     /pseudo
     CDS             complement(5817..6028)
                     /locus_tag="IXT21_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1823 a         1304 c         1021 g         1880 t
ORIGIN      
        1 taaatcatca ctgagctctc ccttctatag attaattaag ccgctttcca atgaaaaggt
       61 tacttcactg tctgccagca aaagaaaatc ctgtcctttt tctgtctcat aaaaagttgt
      121 actcatagtc agacgatcat ttagaaaaag ctcaatagct gaagtatcac ggaaaatttc
      181 gatggttaga caattatttt ccaaaggaat cgtcacactg cgctttttca acacttcctt
      241 ttcagctcct gataaatcat acggcatgtg ttccctggaa gcagtaaagc actcccgttc
      301 aacatcatag ttaagcagta atgtatggtg gggtcccttc gcatagtgaa gttctagttt
      361 cgttgcctgt tttaaattga tttccaagcg taaatagtgc tggtcatata ctaagtcctt
      421 cagtaagaat cgattctctt cagtcttcgc ccattcaatc ttttcctgcg gatcaattac
      481 ttgataaatt gatgaaactg gccgttgcaa caattgatta tcagccaccc tcagctctct
      541 aggcaaggtc atggaacctg accaaccatg gcccagatca ttcgtaggta ttttacgctg
      601 ccacatttgc atccaagcag tcattattct ttggcctgct tcgttttcac aggtctgggg
      661 ggcataaaaa tctaaaccgc catcgatttc atgaaaattc tctactagaa atttgcctgt
      721 ttgccaatcg acagtaccaa tgaaagcaac agttgagttc gtattttggt aagcattgcc
      781 ttgtggagtc atttgaatag gagaaagaat caagacttct ttgccatcta gttcaaacaa
      841 gtctgggcat tcccatacaa tgccttgttc tggagttccc tccagcaata cggaataaaa
      901 ctcccaagtt agtaaatctt tggaatggaa catcacaatt tgtccacgag catctgcagt
      961 tttagtagcg actacagtaa aatagtcttc ttttcgctga aacactttgg gatcgcggaa
     1021 atcttcggtc gttgcaacag aagctaagtg atcttctgcg atgattggat ttccggcata
     1081 tttttcaaag tgaatgccat cttctgaaac tgccaaacac tgaacttgtc gaactttccc
     1141 ttccataaca ttgtgtcctg tataaatcaa atacagcttg ccatcctttt ctatagcact
     1201 gccggaaaaa cagccttcct tgtcatagct ttcacctggt gccagtgcta ctggcaaatg
     1261 ctcccagtga attagatcct tgcttttagc atggccccaa tgcattggtc cccagatgct
     1321 gtcgtaagga taatattgat aaaacaagtg atactcctct tgataataaa taaaaccatt
     1381 tggatcattg atccagccaa tcggagccat caaatgaaat ttattccggt attcttgatt
     1441 gactgtttga gcatttttct caataaactc attggccttt aaaagttttt gcaacattca
     1501 ctattcactc ctattttata cccgttccag aaccgccaag tccctgcaga atatattttt
     1561 gtgcaacgat ataaacaata atcagtggaa tcgttgcaat ggttaaaaca gccatcactt
     1621 gattgatata aactggctgt gtagtattga tcgtagtaat tgccacttgc attgaaaatt
     1681 tgctgctgtc agttaagacc attaacggcc aaatataatc attccagctg gagataaagg
     1741 ataagacacc tactgtggct atggctggtt tagacattgg cagcatgatg tgaaagaaaa
     1801 ttttaacaat atttgcacca tctaatttcg ctgattcaat gatttcttct ggaatcgcga
     1861 taaagaaatt tctaaacaaa tagatattaa acgcgctggc catcgctggt aaaatcactg
     1921 ccaagcgagt attcactaag cctagtgaat taacgatggt aaattgcgga attaaaaccg
     1981 tctccatcgg gacaatcagc agagctaaca aagctccaaa gataattttc ttgcctgaaa
     2041 aattaacctt cgcaaaggca aaacccgcca aagagtttac aatgattgac ccacaggcaa
     2101 aggttaaacc ataaaaaata ctgtttaata aataagttcc cacactgaac cgggaaataa
     2161 cttcttgata agttttaaac caattagctg gattcaaact aggtaaaaaa gctttgaagg
     2221 aagttaaatt ttgatagaca tctgcttcag gcttcataga ggaaactacc atccacagca
     2281 ttgggaaaat gaataatgcc gctaaaagga acagcaaaca atattctacg atcattagcg
     2341 gtccaaattt cttagtcatg ctggatcatc ctcctttacc aattttcttt gagcaattgt
     2401 aattaaacca atcatggtgg taaagattaa cgcaatggag ctggagtagc ctaccatacg
     2461 gtctgtaaat ccagtttggt aaatgtaata aaccattgtc atagtagaat tcattggtcc
     2521 accttgagtc attaccattg gctgaatgat tagtttaaag gccgaaatca atgtggtaag
     2581 taaaataaaa atcgccgtgg gctttaataa tggcatggtg atatagcgaa attgctgaaa
     2641 cttagaaaaa ccatcaattt cggctgcctc ataaacgtct tgcggaatgt tttgcattcc
     2701 ccctaaaaac agcagcatct gatatcctgc accttgccat gccgatacga aaacaagcgt
     2761 atacattgct tgtttgggac tagtcaaaaa aggctgcgaa gcaattccaa tctttgcaaa
     2821 tagcgtatta accagacctt cattaggatt taacaagtac aaccataaaa tcgaaataac
     2881 gactaaggac atcacgactg gcgcaaaaaa ggctacttta aaaaagatat ttcctttacg
     2941 atttttgttg ataataagtg ccattcctaa agctgcccct aattggaccg gaataatcca
     3001 aacaacaaat ttagccgtgt tccaaatact ttttacaaaa attggatcag cgaacagtcg
     3061 cttaaaattt tctaagccga taaattgtct agcatctggc gttaacaaat aataatcagt
     3121 aaaggcataa taaattacca tgattacggg tatgatcaga aaaatcgtca gcagcagcaa
     3181 agctggcgct agaaatccgt aacctatcaa attgtctttc attgtcggtt tttttaacac
     3241 ttgattggcc ccctttagaa acttctattt tagtgaagta tcaattacct gttgcatttc
     3301 agcagcgcga ctatctgcta ctttttgtac atctgggttt tcctcgaagt agctaatatc
     3361 ttgaactgct tgttggaatg ctctagatac ttgcggataa gctacaacca ctgggcgggc
     3421 atgtccagaa atttggtttt gcttaattaa tacctgcatt ccttccgaca cttcatcttt
     3481 aattaaatca actgttgaat ttctgatagg tagaacactg ttccctaaac tcataatacg
     3541 actagattct gtactggttg caaacttaat aaaggccgca gaaccatcct ttttctctgt
     3601 tttctcagtc attgctaact gccagctgcc ggttggtgaa actaactccc cagttgcatc
     3661 tgaaacgggg taaggcaaaa taccataatc aatctcttga tagttttcct ctaaatctgc
     3721 cattgtccaa gaaccgctca gcagcatggc atatttgcca gtttcaaatc ctttttcaat
     3781 aggactgatt gtggtgtaac ctttttcaac caattcttga ataaattcca acgcttcaac
     3841 ggatttttta tcgttaaaat agtcttcggc tttggttcca tcagcattaa cgacatcgcc
     3901 gccattcgac catacaaatg gtgtatatgc gtaggtcagc atttcgtctc ccgactccat
     3961 ttgcatatca attgcaggcg ttttgtattt atcagtcagc ttttggcaaa ttgcttgaaa
     4021 ttccgtccaa gtccaaggat cttctaacgt cggcaattga tcttctgtaa ttccagcatc
     4081 tttgaacaac gacttattgt aatagacacc cacactggac tctgagaaac caaacgcata
     4141 cattttgcca tcgtaggttc cttgctgaat aatgctgtct aatacatcat ccatgtccgc
     4201 atctttcaaa taatcatcta atggagcaat gactcctgaa tgagcataag ctgcagtatt
     4261 cggtccatct aacgtaataa catcaggaag ggtattggtt gtaattgcag catttacttt
     4321 atcctcatat ccaccgccgc ttccgctacg aggaatatac tcagtaacag ctttatattc
     4381 accctcgtat tcatcattaa aattgttcac aatctctgcc caagcttttc cttcaggcgt
     4441 ttcatcagaa aactgaaccc aaatcttaat ttctcctgaa tcactgctgc ctgaactttt
     4501 ctccttactg ccacatcctg caagtcctcc agctgttaat gctaaaattg ctgcaattgc
     4561 taacattctc cttttcatcg atgttcctcc tattttttga aacgtttccg ttttattctc
     4621 caattaattg gtaacgtttc caattaattg gttttaagat atcattgaaa ccactttctg
     4681 tcaacctatc tcaaaaaaaa atcgttataa accggttttt tactctttag tcgtttctcc
     4741 agcgtgaaag aaaaccggta ctaccacacg cttaggcggt tcttcattta aaatcatcgc
     4801 catgagtgtt tctactgaaa ccttggccat ttcctctatc ggctgtacaa tcgtcgatag
     4861 taaatagtct ttttgcaacg atagcttgac tccatcaaaa ccaataattt gaaaatcctt
     4921 aggtgcttcc ttccctagtt ttttttgggc ttttatcaca tccaaagcca tgaaatcatt
     4981 gatggtaaaa atgctatcaa tttcaggatt atccgaaaaa aaagcgagca attgttcctc
     5041 caaattgcta attggttcag gcatatccaa taccttcaca gctattttat ttttctcaca
     5101 aatattgtaa aaaccagctt tacgattgtt tgtttcgtta gggtaaggtg aaaaaccccc
     5161 aacatagcac aagtttttac agttacgagt aagtagttct tgagcggcaa tttgcccacc
     5221 taaataatta tctgccgtta cataagaaac ctgttccgaa aaatggcggt ctatactgac
     5281 aaatggtaaa tttgatgata catatttatc aatgtctgaa taggtaatac ctataattcc
     5341 gtctacttta ctctgcttga ccatttggat atattctttt tctttttccg cactgccgtt
     5401 cgcattacac aaaaaaagct tataattttt ttggctgaga gcttcctcta catagtaggc
     5461 aaactcggaa aagaagggat gccaaatcgt aggtaaaata agagctattg tattgctgct
     5521 attcgttttc atacctctgg catattcatc aggctcataa ttaagctcac taattgcagc
     5581 cattactttt tctttggtaa cctctttcac ctttactcca tttattactc tagataccgt
     5641 acccacacca actcctgcta acgcagcaac atccttcata gtagttttca cttcacgaac
     5701 aactcctctc aaaagaattt caggtattat tataccaaaa gaagaaaacg attccaaatt
     5761 agcaggaata tttttatttt aaaatagata ttagacgtga agcttttcgt aaaaaaattt
     5821 tcgtattcag atttaccgga ttgactctgt cgccaatata ggccggcata ctttgtcaat
     5881 tttatttcat cagaaaaacg ttcaattgga ccaatttcag cgagtattcc agcagcctaa
     5941 acaggaccaa tacctgggat actggttaaa cattgatact ccggtagcac cacgactaag
     6001 tcgtcaatgg ctttatctaa gtctttaa
//
LOCUS       DACGLH010000124         5919 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.130, whole genome shotgun sequence.
ACCESSION   DACGLH010000124 DACGLH010000000
VERSION     DACGLH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.130"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..636)
                     /locus_tag="IXT21_002794"
     CDS             complement(<1..636)
                     /locus_tag="IXT21_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4870145.1"
                     /translation="MQAPIKKICVHFETKQWRNKMVATVTLYLTFTLILDLGIYLFFS
                     KQLLVKNSFGCIALMVVTLLLPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFF
                     AITGLSFFLVFDLMDYLGKRHLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPT
                     EHYFGGICILLSWGVIYLVLGSVHFRSSNYLLLSLLSIVISFFSLFFSIMLI"
     gene            637..1377
                     /locus_tag="IXT21_002795"
     CDS             637..1377
                     /locus_tag="IXT21_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4870146.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1463..2221)
                     /locus_tag="IXT21_002796"
     CDS             complement(1463..2221)
                     /locus_tag="IXT21_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ4870147.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2218..3246)
                     /locus_tag="IXT21_002797"
                     /pseudo
     CDS             complement(2218..3246)
                     /locus_tag="IXT21_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(3266..4453)
                     /locus_tag="IXT21_002798"
     CDS             complement(3266..4453)
                     /locus_tag="IXT21_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870148.1"
                     /translation="MKNYNRISANNQLPMIESLRVPTNADKKIYGHVVVPEGVEAKPR
                     DAWTGEVEVTLRITYADTTKSSVEITALTQGQTDENEGAGYNPWGEGEQGGLFEVQVP
                     NDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVDVIPPEPANLSTTLINAASRTIT
                     GTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPENQLFAGDVLEIVLNDQAGEATAK
                     GVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKIVVEGGLSFLAPSKLDFGQIKAT
                     GLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQPFTNDAGFSMQDVLYFKKGEEY
                     QLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLVLTKENQIAGNYSAEITWTLADA
                     PTE"
BASE COUNT         1685 a         1259 c         1046 g         1929 t
ORIGIN      
        1 gatcaacata atggaaaaaa acaaagaaaa aaaactgatg actatcgaca aaagagatag
       61 aagcagataa ttagatgagc ggaagtgaac acttcccaat accaagtaaa tcacgcccca
      121 gcttaataaa atacaaatac ctccaaaata gtgttcagtt ggataagtaa aaagcaagtt
      181 gagaaatctc gtctgtttgt ctatactttt tactaataga tagaaaataa atgcgataag
      241 tagtgcaaaa aaacttgctg ttccaagatg tctctttcct aaataatcca tcagatcaaa
      301 aaccagaaaa aagctgagtc cagtgatagc aaaaaaaaga ttactgatca aacagctgat
      361 aaacaggttt ttcgtagtaa aataaaaaag aaacatcaat aataagccaa ctaggtaagg
      421 aaagctcact ttagggatag gaagtaaaag tgtcaccacc atcaaagcta tacaaccaaa
      481 gctgtttttt actagcagtt gtttcgaaaa gaataaatag atacctaaat ctaaaatcaa
      541 agtaaaggtc aaataaagtg tcactgttgc gaccatctta ttcctccatt gttttgtttc
      601 aaaatgtaca catatttttt taataggagc ctgcatatga aaaagtattt tatcatagaa
      661 gataaccagt ttcacagcga atggatcatc agagaattgg tcaatcatca tatcgagaat
      721 atcgtgcagc tcacttctat ctctcaaatc aatcagttga tccagttggg cactattgat
      781 aatcacgatg ttttctttat cgatatcgac ttgaagcaat cactcacagg tattgatgtc
      841 gccgaacaaa tacgtaaaaa aaatgcacat agtaatattg tttttttcac cgcttatacg
      901 aattttgcag tagagatcat caatcggaaa atccttccga ttggatatat cagaaaagac
      961 aggaatttat ctgaacaaat tcgtcaagtg ctagaacaga tcaattggaa agaacagcag
     1021 atcgtaaatg tcgataaaat ctttattgat caaaaaggtg gaagtctcgt cctgtatcct
     1081 cgggaaattt tatatattga aagtatcaaa ggtttgaaaa accaagttat ggtcaaaaca
     1141 actacagaag ataaattagt gaatacttct ttaaaacaat tgaagacaga gttagaaaag
     1201 tatgcttact ttttactttt aaaatcttac tgtatcaact tatcgcaatt gcagaaaatt
     1261 gattctgctc attatgtgtt agaatttttc aacggtgact ccttactcgt tggtcgaaag
     1321 atctttgaaa agacaaagga acgtttccat gacttccaaa aaactgcttc gagttgatga
     1381 ataagagctg agacgtaagc atagcagatt tcagggagtg ggccttgaat cggctccact
     1441 ccctgaaatc gttgatatga ttttacgctt cctcttctgt aggagcatct gttaacgacc
     1501 atgtgaatgt tgaaacgtag ttgtctacag cataattgga tacaggaaca aataattcca
     1561 ctgattgtct cccttgttcc acattctctc ctaatgaata aatccatgtg ccaatccctt
     1621 catttgctcc tgcagttact agatctactg cctgatctgc tgggatttcg attccttcag
     1681 cgccagttcc agaaacggta gatggcattc cactgctatt agttgcgcta tttcgaatca
     1741 cggcgttttt gaatttcacc tttgaaccag ttagtttatt cgtatcagat gttgtaccaa
     1801 taaattctgt tcgtttgact gataatttcc aaccggcata atttccgctt ttatccgtta
     1861 cttgtacgaa attctgtact tgtgtttgtg ctccagtact gtccgttccg ctttgtaatg
     1921 cagcatatac tgtttttgct gtacctaact tgattgttcc aaaatcaaga tctggagctt
     1981 ggtcaatcgt cagtaatccg gatgttccct gatttggttc gtcaggtgta aaaggagtat
     2041 caggatcatt tggatcaaca ggaggaactg gaacaacgtc ttcaggattt gcagcatctg
     2101 gtttggtcac agaaatcgtt ccttctgtag tggctgagct cttattctca gcaaaaacac
     2161 tttgtgtaca taataataga cttccagcta gaacggtact taatgcaatt gtttttttca
     2221 tttctttttc cttctttctt ttaaaataaa taatccagca aataagccaa taagaatgac
     2281 tccaacaagt atcagccatc gccagtcaaa tgtttctggc tgatttttta aatcaatatc
     2341 cttttcattc aattggtccg ctgcatcatc agttatttca aaatcggtat ggagattcca
     2401 gcgtttttct ttccaagcac ccttcacaac cgcttgataa tgtcccggag ctaatttttt
     2461 tccttctaat gggacaagta aatcaaaatt tgtattgggt gctacatcta ttttttcttt
     2521 ttttattgaa aggtaaggct cttttgtttc ttttttgtag atttcaaccg atgtagtcaa
     2581 gccgaacata taagaagcat ttggattttg aagattcgcc ttaatcacat ttcttccatc
     2641 gctttgatcc gcataagctt tttttaattt caaatctggg gaagactctt tttcgctatt
     2701 tcaaatcaag attgctttca cttgagaaaa ctgattgacg acaccaactg tacttttgtt
     2761 agaaacgttc tctttttttt cttgaaacgt gatccctcct gcgaccacac cagccagctc
     2821 tttttctggc gctttcagtg aaagtgtctc agtgaccgca ctttgaggag gaacagttat
     2881 ttctcccact gtttttgcta agtttttcat atcataactt aatgtttcat caaatttact
     2941 ttttgattct ccatattcga tcacaccgtt tgaattcgtc gtagcggagt ggatcaatgt
     3001 ctcaactgtt actgatttat ctgttcggtt aaagaccgtg taggggattt cctgtgtttt
     3061 cccaggctcc atccttacat ttatatatgt tttgttctta tcaatctgat tagccggtaa
     3121 ttcaaaatct accgcaaaat tgaattcgga ggcatggaca ggtgtctgcc aaaggatgaa
     3181 tccaaggaga aggatcataa gtaaagagca gttgtttcga tgacttttat tccatattcg
     3241 attcatcaat catcctcctt gatctttatt ctgtcggcgc atctgctaat gtccatgtta
     3301 tctctgctga gtaattacca gctatctgat tttctttggt taagacaagt ttaaacgccc
     3361 gaccgttatt taaatattct gaataatctg cttgcacatt cgttgaattt tgtcctctga
     3421 caatttccag ttcttcttca ttcactaatt gatactcctc tcctttcttg aaatatagga
     3481 catcctgcat agaaaatcct gcatcatttg tgaaaggctg agataatttt atcgttaaga
     3541 tccacggatt tttcgtttct cgctgatcat aaatccataa tttttcctgt atatccattt
     3601 ggccaaatgc ttcttgtgtc aaaccagttg ctttgatctg accaaaatcg agtttggaag
     3661 gggcaagaaa tgagagacca ccttctacta cgatcttcgt agcaggtgca aaaggcgggg
     3721 catcatgata aacaactggc gtatcacttg gattgtgatt cccaatttca tcgttggtac
     3781 ttggcttgtt ctgcacaccc ttagcagtag cttctcctgc ttggtcgttt aatacaattt
     3841 ctaatacatc tcctgcaaaa agttgatttt ctgggataga aagaacaaat tcccctgtat
     3901 catcaatagt tgtttctgac aaaacagctt gatttttttt caatgaaacc aaggctcctg
     3961 cttcacctgt acctgtgatc gtgcgagaag ctgcattgat caatgtggta cttaaattag
     4021 caggttcagg aggaatcaca tcgactgtcg ttttggcaga gtccagttct gtcacttctt
     4081 ggccacctct tactttttta gcagcaacaa cttgcacttg atcctccgtt tttaatctct
     4141 catcattggg tacttgaact tcgaacaagc cgccttgttc gccttctccc catgggttat
     4201 atcctgcccc ttcattttca tctgtttgtc cttgcgtgag agcagtaatt tctacagatg
     4261 atttcgtcgt atctgcgtaa gtaatacgta aagtcacttc tacttcgcct gtccaagcat
     4321 ctcttggctt tgcctcgaca ccttcgggaa caacgacatg cccatagatt tttttatctg
     4381 cattcgttgg cactcttaag gattcaatca tcgggagctg attattggca ctgattcgat
     4441 tataattttt cattccggca cctgtctgag caaattcatt tgttaaacct gcactagggc
     4501 ttgatacgaa attgcctaag tcggtcccat tgatcgataa gtctgtcata tcagaaaaat
     4561 aatccgggtc tttttcaaag ttatcgaatg ttgcgcctgt ttttcgccat aaagcgattt
     4621 ctgaatgggc gatctttaaa gaagaatctc catttccttg gaatacccag ccgcctatgc
     4681 cattggtctc gcttccaatc cgtttgtttt ggaaatcaaa ataggtagga gattctaagt
     4741 ccacattcaa cacatcccca ctgatgatgc cggtcttatc ttcccctgtt ttcccgacca
     4801 tgactaaata agttttttca cctgccgtaa aatttaaatc aatatctcca gttgtatcta
     4861 cagttgctcc ggaatctgcg cgtatatcga tatttgattt ttctccagtg actgtaaaat
     4921 cagctgaacc tggactttct agaaaaccag aagcgtcata aaattggatc gcttgatttc
     4981 cttgattgag tctgttcacg taagatttgc catcacctgt atttcttacc ctcaatgagc
     5041 ctccattggt tactgtcagc ttttgcgtac cattctcaaa acgaactgcg ctggaacgcc
     5101 cactctgctt atcgatgtcg ataactgatc cggctccttc tacgatcaaa gaaaggcctt
     5161 ttcctacaaa acgcatccca ttgttcccgg gttgatcgtc atctccagcc attttgaccg
     5221 agatttgtcc accacttgat acatggattt gactagaatc tccttgcaag cgaaatccgc
     5281 ccgtagttgc tgtattgatg gtgattttcc caccattcga tacagtgact ttcgatcctg
     5341 aacctctcat ttggatgatt ccgcgattgt tgtcattggt aatattgatc tttgtattta
     5401 ccgaaaattc gcttcccgaa ccactgactt caaacatgac attaccaggt agcgtatcga
     5461 ctgcctctcc ttcatcgcta acgccatctg cccgatcata atcaagagag acctttgaac
     5521 cgcttaagat atggattcct ggcgctgttt ttccactacc agtctcatct cctgaatcta
     5581 gaaaaccgta gaatttccct agatacggtc cataaagttg actaccattg atcacattca
     5641 attgatagct tctacaaaaa taggctttac ccggagcggc attcagtcct tccttcactg
     5701 gttggacatc caacacaccg gaaacaccat ctaatgtgac agttgcacga ggagcgtaaa
     5761 tcagtcctaa tttggaccca tcatacaaat caagtgtccc ggtaaatttt atttctcccg
     5821 taaaatgata tctgcttccc caaaaatcgg tcatataagc aatgataatg gcatttccat
     5881 cgatatttgg tgtcaatcca cctggatgtg cagctgttc
//
LOCUS       DACGLH010000125         5884 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.131, whole genome shotgun sequence.
ACCESSION   DACGLH010000125 DACGLH010000000
VERSION     DACGLH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.131"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(74..1168)
                     /locus_tag="IXT21_002799"
     CDS             complement(74..1168)
                     /locus_tag="IXT21_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ4870149.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1357..2118
                     /locus_tag="IXT21_002800"
     CDS             1357..2118
                     /locus_tag="IXT21_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4870150.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="IXT21_002801"
     CDS             complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="IXT21_002801"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ4870151.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2893..3648
                     /gene="fabI"
                     /locus_tag="IXT21_002802"
     CDS             2893..3648
                     /gene="fabI"
                     /locus_tag="IXT21_002802"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ4870152.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3758..4771)
                     /locus_tag="IXT21_002803"
     CDS             complement(3758..4771)
                     /locus_tag="IXT21_002803"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ4870153.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4936..5856
                     /locus_tag="IXT21_002804"
     CDS             4936..5856
                     /locus_tag="IXT21_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ4870154.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1789 a         1174 c         1054 g         1867 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt
     1321 aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta
     1381 agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact
     1441 tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc
     1501 agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat
     1561 gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa
     1621 cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga
     1681 ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat
     1741 ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt
     1801 tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc
     1861 cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat
     1921 cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat
     1981 actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa
     2041 acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa
     2101 gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta
     2161 ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat
     2221 caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt
     2281 tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat
     2341 ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt
     2401 gatcccaccg atataagctc tttttcctag aaactcttct gattttagga taagtatcga
     2461 acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa
     2521 gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc
     2581 tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc
     2641 cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg
     2701 ccattttcat tactttgact atcaaagtat catattccta aatatttagc aatctttttt
     2761 taatttaaga aaaaatagtt tgacaatcaa aacattttga ctaataatta agaaatcaag
     2821 aagttatttt agctattatt ctctattttt ttacgactat caaattttta tcagaaacaa
     2881 aggagagtca ttatgtcatt tctagaagga aaaaaaatcg tcattatggg cgtcgctaac
     2941 aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga aacaaggggc agaaattatc
     3001 tatacttacc aaaatgaacg aatgaaaaaa tcattagtca aattggtcgg cgaagaagca
     3061 ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg aaaaagcaat gaatgccatc
     3121 ggtgaatatg ctggggagat ccacggcctt gtccatgcag ttgcttacgc caacaaagaa
     3181 gaattgtccg gaaatgtgtc tgatatttcc agagacggct atcaattagc tcaagatatc
     3241 agcagctact ctttaattgc tgtatcaaaa tacgcacaac cgtttcttgc accaaacagc
     3301 gggatcgtta gcatgactta tctaggttca gaacgcgcaa tcccgaatta taacatgatg
     3361 ggtattgcga aagcttctct tgaagctgct atccgttatt tggcagctga gttctcgcct
     3421 aaaggaattc gggtcaatgg aatctcagct ggggcaatca aaacattagc tgtcacaggt
     3481 gttaaagact atcaaaaact gatccagtta tctgaatctc gtacgcctga tcagcaaggt
     3541 gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa tcagcccact ttccagcgga
     3601 atcatcggtg atattatcta cgttgacaaa ggtgtccatc tttcataaac ttttgttgca
     3661 ttctttaaag taaaacatat aaaaatcagg aaaaaacttt ttaaagtctc ttcctgattt
     3721 tttcttttta cgtttttttc aactcattac tttgaaatta ttccacgact tcaatccagc
     3781 cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa ttcatcatac aaccgttttg
     3841 ttactggtcc tacttccgtt tcactataaa atacatgaag attgttattg tgataaatcc
     3901 caccgattgg agaaataaca gccgctgtac cacatgcacc agcttctgca aattgatcta
     3961 gatcatcaat gaaaatatct ccttcaattg cttgcagtcc tagtcgttct tcagctaact
     4021 gcaacaagga atatttcgtg atacttggta agatagaggg cgattgcgga gtgatgaatt
     4081 ttccgtcttt tgtaattcca aagaaatttg ctgcacctac ttcttcgatt ttcgtatgtg
     4141 tggctggatc aagataaaca caatctgagt aatccagttc ttttgcttct gttcctggaa
     4201 gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc agtcccctta ggagcagcac
     4261 gatcgtaatc agatacaata aaattcgttg gttttagtcc acctttgaaa tacgctccta
     4321 ctggcgtaca gaagacgaca aataaatatt ctggagctgg atggacacca atgttttcac
     4381 cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc tgttccatag ggcggcacaa
     4441 aatcttcatt tgcttttacc acttgcttga cagcatcaac gaacatcttt tcagggacca
     4501 ttggcatcag caatcgtcta gcactttgat tcatccgtcg actattttgt tcaggtcgaa
     4561 agagattgac cttgccgtct tttcgacgat aagcttttag accttcaaaa cattgctgac
     4621 cataatgaag cgctggagag ccttcatgaa tcgtcaggta attatcttct gtcaattctc
     4681 cttcttccca ttgtccgtct ttccatttgg caataaaacg ccaaggagtt ttgatataag
     4741 aaaaacctag attgttccaa tcgatttgca tatacttacc accttcgtta ttttctccac
     4801 attttatcat tatccatatt atttgtcatt ttattttcag cttttttcct tcattatagt
     4861 tatttttgct atttcctttt ttgaaaactt cttggtacaa tacagaaaga atcaaatttt
     4921 agaaaagagg attttatgtt ttcattatta gcaactgccg gtacgacaga ctcttctggc
     4981 gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt ttcagcggtt ttgggacggg
     5041 attgattggg acgccatttt ggctgccttt atccaaaaaa gtttatatct gatttttttg
     5101 atcgtccttt tttggattct tcaccgtgtg ggaaaatatc tgattgataa gtcttttaat
     5161 cagtattcta aaaaagccat tctaaacgag agccgcttaa agactttaca tagtctttta
     5221 aatactattt ttcattatac ccttggtttc ttttttatct atgcaatttt atctgctatc
     5281 ggtgtgccga tcggatcact tttggctggt gccggaattg caggtgtggc aatcggtctt
     5341 ggtgcccaag ggttcatgag tgatgttatt accggatttt ttattatcat ggaacaacag
     5401 atggatgttg gggactacat caagctagca aacttaacga ttgaaggaac cgtcgtatct
     5461 gttggtatcc gtacccttca gctgaaagca acggatggta ctgtccattt catcccgaat
     5521 cgaaacatca ccacgatcag caatctctct cgtgcaaata tgcaagtact tttagatatt
     5581 cggattgttc ctgaagaagg atatgacaaa atctatgaga tcattgatcg agtaaatcgt
     5641 acattagctg aaaaatacca agaagagctc caaactgaac cttctatctt tggattagtg
     5701 gatcttggaa acagcaattt tgcgattcgt acggtctgct acgcattgaa cggaaaacaa
     5761 tatgccttaa aagaagagtt cttgagctcc tatgtcaaag aattgacagc ttctggtttc
     5821 acgattccga acagtccaat tgctgtcgga aaataatttg gacaaacgat gcaacgaatc
     5881 tcac
//
LOCUS       DACGLH010000126         5877 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.132, whole genome shotgun sequence.
ACCESSION   DACGLH010000126 DACGLH010000000
VERSION     DACGLH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.132"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            641..1375
                     /locus_tag="IXT21_002805"
     CDS             641..1375
                     /locus_tag="IXT21_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4870155.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IXT21_002806"
     CDS             1391..2560
                     /locus_tag="IXT21_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4870156.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IXT21_002807"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IXT21_002807"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ4870157.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IXT21_002808"
     CDS             3650..4576
                     /locus_tag="IXT21_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ4870158.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IXT21_002809"
     CDS             4595..5845
                     /locus_tag="IXT21_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4870159.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1204 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DACGLH010000127         5781 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.133, whole genome shotgun sequence.
ACCESSION   DACGLH010000127 DACGLH010000000
VERSION     DACGLH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.133"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(20..2191)
                     /gene="copB"
                     /locus_tag="IXT21_002810"
     CDS             complement(20..2191)
                     /gene="copB"
                     /locus_tag="IXT21_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ4870160.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2211..4397)
                     /locus_tag="IXT21_002811"
     CDS             complement(2211..4397)
                     /locus_tag="IXT21_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ4870161.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4397..4606)
                     /gene="copZ"
                     /locus_tag="IXT21_002812"
     CDS             complement(4397..4606)
                     /gene="copZ"
                     /locus_tag="IXT21_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ4870162.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4619..5059)
                     /locus_tag="IXT21_002813"
     CDS             complement(4619..5059)
                     /locus_tag="IXT21_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ4870163.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5133..5672)
                     /locus_tag="IXT21_002814"
     CDS             complement(5133..5672)
                     /locus_tag="IXT21_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4870164.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1495 a         1302 c         1008 g         1976 t
ORIGIN      
        1 tcacaacctt ttttcattat tatctgattt tcaatgtcaa agcatttaga gctacaacta
       61 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
      121 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
      181 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
      241 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
      301 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
      361 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
      421 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
      481 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
      541 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
      601 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
      661 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
      721 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
      781 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
      841 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
      901 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
      961 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     1021 ctattgatgt ggaacgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     1081 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     1141 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     1201 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     1261 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     1321 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     1381 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     1441 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     1501 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     1561 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     1621 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     1681 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     1741 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     1801 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     1861 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     1921 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     1981 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     2041 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     2101 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     2161 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     2221 atgtgtcgat ttaagcacag ggagttcaac aatacactga cggaactaaa cgccatcgct
     2281 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     2341 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
     2401 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
     2461 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
     2521 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
     2581 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
     2641 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
     2701 cctttattct ggagctgctt gattgcttgt ttgacttcta gtttgatttg atcagccacg
     2761 gcaatcaacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
     2821 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
     2881 ttacgtgtac ccgcaaaata gcgtactccg ttaatcgtac cgctgattcc agcacctggg
     2941 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
     3001 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
     3061 tctttagaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
     3121 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
     3181 attccatttc gtgcaccaac acccgttcct accataatag ctgttggagt cgctaaacct
     3241 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
     3301 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
     3361 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
     3421 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtaacttcc
     3481 atgtgaagta gaccattcgt attgattgtc ccgccaaaga gcgtgtcatc cggattcttt
     3541 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
     3601 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
     3661 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
     3721 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
     3781 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
     3841 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
     3901 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
     3961 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
     4021 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
     4081 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
     4141 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accatagccg
     4201 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
     4261 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
     4321 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
     4381 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
     4441 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
     4501 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
     4561 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
     4621 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
     4681 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
     4741 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
     4801 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggataataa atgaattttt
     4861 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgacccagc aaagttttga
     4921 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
     4981 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
     5041 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattatctta
     5101 tgaatacaga ttacacttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
     5161 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
     5221 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
     5281 aaagataaaa attctaagta ttccctgttt agtttctccg aaaatgattc aggcatatct
     5341 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
     5401 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
     5461 gtagaagtat aatttccttc aacgatccat gacggattag ataccataaa caattgctgt
     5521 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
     5581 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
     5641 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
     5701 ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt
     5761 caaagtccct tgtttcgaat a
//
LOCUS       DACGLH010000128         5769 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.135, whole genome shotgun sequence.
ACCESSION   DACGLH010000128 DACGLH010000000
VERSION     DACGLH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.135"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(1025..1423)
                     /locus_tag="IXT21_002815"
     CDS             complement(1025..1423)
                     /locus_tag="IXT21_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870165.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1639..2694
                     /locus_tag="IXT21_002816"
     CDS             1639..2694
                     /locus_tag="IXT21_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ4870166.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2714..3067
                     /locus_tag="IXT21_002817"
     CDS             2714..3067
                     /locus_tag="IXT21_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870167.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3249..3545)
                     /locus_tag="IXT21_002818"
     CDS             complement(3249..3545)
                     /locus_tag="IXT21_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870168.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3549..4367)
                     /locus_tag="IXT21_002819"
     CDS             complement(3549..4367)
                     /locus_tag="IXT21_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4870169.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4593..4814
                     /locus_tag="IXT21_002820"
     CDS             4593..4814
                     /locus_tag="IXT21_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ4870170.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4816..5082
                     /locus_tag="IXT21_002821"
     CDS             4816..5082
                     /locus_tag="IXT21_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4870171.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5195..5734
                     /locus_tag="IXT21_002822"
     CDS             5195..5734
                     /locus_tag="IXT21_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870172.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
BASE COUNT         2036 a          953 c          861 g         1919 t
ORIGIN      
        1 agtttttacc tattcccaaa atccatttat tcgttatttt tttcactgat taagaataat
       61 tatttgatgc ttaaattagc taaaaaagcg atttgttact gatataatca tgatattgca
      121 gctgaattag gcggaaagat aaaatgttcg caaaatcaaa gtatcaatta tcttgttttt
      181 ttataaattt gtaagtaaaa atctaaaaaa gttttctttt aattggtata tgtattaaaa
      241 tcacatttaa tcacccataa agtatgcgct ttcatttttt tggatgagtt taatatcaat
      301 aaataacgat gtaaaaatca tttattatgc tcttttataa ggatattaat tgatctaatt
      361 tgaaaaaaat caaaatttaa atgttttaat attacaaata aaatcatgtt attgtaaaat
      421 ggtataaaaa agctaaatat tctatttttg ttattttatt agcaggaaaa tttaaatagt
      481 tttacaagaa tccgtagaaa tatattggtt ttgccttcag attaatactg gatcatatta
      541 atctgaatat aatatatgaa ctaataaata gcttaacctc gctattttgt tttttctgat
      601 cactatctat acagcattag taaatagaat tttaaaaata aaaaaattaa gacaaaaaag
      661 tatcaacata aacatataca tatacacgtt tatgttgatt cacttaattt gtcctttaaa
      721 aagcacttat tttaatataa tgttatttta taatcttatt acaagctgat tgttaggcgt
      781 tgtcctttca ttacatttat taacggttat tttattgttt aatttattaa ttatttttga
      841 tctaaaactt agcttaacaa ataaataatt aaccaaccgc cagataaaaa tgcgatcatt
      901 gctaatacat aagcaaagat aaaaatatgt tcgatcagct taaaccctga tgcgattaag
      961 cagaccaagt aaaatagaaa ttctcttaca gataatggta attttctaac tagcatactc
     1021 ataattaaca ccccaaagta atattgtatt ttgaacattt tacatccaat acagcatcaa
     1081 tcaaccattg ccaatcaaaa atacaaggct tcattgaagt atgtttttca gatgaaaaga
     1141 tataatataa tttcttcgga ataatatttt cagttgttgg gatcaagcta aatctaatac
     1201 cgtctaactc atagctacca gctttctctc cattaaaaat ttccttagca ctttcttcta
     1261 aagaatcaaa atatttttcg acagcaatct ttgaagcttt tactattgaa gcatttccct
     1321 tttcgataaa atctgctttt gttttaccga caataagtaa ttcttgatca gtaaaatctt
     1381 ctaaaataat ggtagtgtaa ttcttatttc ttctcaatcg catcaggatc actccttaat
     1441 ataatttatg tttttataat atcataataa gataaatttg caagcattta tttacacctt
     1501 tttatttaca ctttattata attaattaga cttttatgat tgcatttttt tcttataaac
     1561 agttgcaact aatagtaaat aggtgtaaac tattttcgta ccgaaatacc ataaacacaa
     1621 acagaaagag gtcataccat gaaagaacca ttattgtttt ccgttgactt agggaataaa
     1681 tcagtaaaag aaatgagtac acacgtttct ccactctcac ttccctctcg tattttgaac
     1741 aaaaatatca ttaattcagg acgtttttct aatataggtt cctcattaaa acgatcacta
     1801 aacgtttctg aatatagcct agtaaacact actgaaagct attactttgg gaaggatata
     1861 cacgagctag gaaaagatga ctacgtgata gaatcactag gttttgggat cagcagatac
     1921 aagcaggaaa cctaccaaaa tatcctatct ttctctttag ctaacctagc acgtttagaa
     1981 gatcaatctg atgacgtttt aaccgctaat ctagttttag gtctgccttc atccgactat
     2041 accgaaacaa tcgtcaaata cgtgatgcaa caagccacac gacaacattc agttgaagtt
     2101 gacggtaaag cttataatat aaacgtagat accgtttggg tactgccaca gccgattgga
     2161 acactatata acctcctact tgctgatgat ggtagtgtac aagatgaaac attgatcagt
     2221 cagaagatcg gttttgttga tattggtggt ggcacgaaac tttttgatgt tgtccaaaat
     2281 tttgaaattg acgaaaacaa ccgtgaacaa aaggataccg gtatttttac tttatataat
     2341 cgtatcgtaa atatgcttga ccttccgatc agtcctaatg ctttccaact tgaaaagatt
     2401 atccgagaag gcaaagaaaa aggcgtatat ctatttaaac caagcggaaa tgaaagtttt
     2461 gatatttcag aagtagttga aagagaaata aaaacataca ccaatatggt tgttgcagaa
     2521 gttttagcta cattcaaaaa cttcacaacc ttagataaag ttgtctttac tggtggtggg
     2581 gcttctatta tcgacaaaca acttgtaaaa gataaaattc cgcatacctt gtttgttgaa
     2641 gacagtgaat tttctaatgt aaaaggcttt tacaagtttg gtttaactca ataaaacagc
     2701 gaaggagatc aacatgaata agggaaaatc agagcgttat gtagttataa tcaatagaga
     2761 aaaggttagc gagttacctt tagcagagtt tcttgaatct gaatatgaaa cactaggcgt
     2821 taaatactct cagacgatca aaaaagcttt gaatctatat cggcgtgtaa agaacaatga
     2881 agttacaatt attgaagcag gcaacgatag aaacgatagc aaagtagctg aagtaaagaa
     2941 taaaccaact gatcataacc ctattcaaca agcgcatcct tcaacggtta catccgatca
     3001 ttcagcaaag aaagaaaagc caatgctaaa agggttcaat gcaccaagta acaaagactt
     3061 actttaaaaa taaaaaggcg aggttacaat acctagcctt tttattatgt acaaatacaa
     3121 acataaacat atacgtatac atgtatataa acatgtatac gtatatatcc ataaacacgt
     3181 atacgaaatt tagttaagct atacctaacc gcttattcca ataaaaaaag tgatcagctt
     3241 taaactgatc actcaccaaa aacttcatca tacaagttat ctatcatttc accaagtaaa
     3301 gctgaatctg atcgaattcc taacttttcg ctgattttag atagtttctt tctttgtgat
     3361 ggttgaagcg tgaattgata atttttcttc ttttcagaac tatcactttt tagtttagct
     3421 accaaagaac ttgttgtttc tttaggtgtt tgtttaggtt cttcaactgc ttcaaccact
     3481 tcaggaactt cttctacttc agcttcagcg aattttttaa atctattttc tttcacttca
     3541 atcactcctt attcttgtgc cgtatcaatt attttgttgt atagcgtaaa cgtgctttca
     3601 aagaaatcct tattcttacg atagatattc ttatccttag cgaactcagc aatattctta
     3661 ccttctaagc tagttgcatt aagcaattcc ttctcttgta catatccaag ataattagat
     3721 aacgttggta ccaattctag gaactcacga cttgaattag tattatgttt gatcctgcta
     3781 ccgatcgtag cgtatttagt ttccatcagt gtttcaccag tcttaatatc aatcaactgt
     3841 tttgctagtg catcaatatc tttaccaagc ttaagcaaag cttcaaaacc atctttagat
     3901 ggatcagaaa tgcctacgac aagatcagat acagcccaag cattttgagc tagtagacct
     3961 gcatcattat gagtatcaat cagaattaga tcatacttct ctttaagctg ttcatagtta
     4021 acaacgatcc atctaaacag tagggttctg ttgttaggca tatcacgcat ttgcatctca
     4081 acttgtttta aatcttcacc tgctactaaa atatccatgt tctcatttat agctaaaggt
     4141 tcagctgatc catgaagata taaatgattg attgtattct ctcttttcgc tttactgaaa
     4201 gcatcaggaa atcgttttga taggttttgc gacttatctt catcaatata cagcaccttc
     4261 atacctaaat tttcgacagc atatccacct agattgtaga ttgttgttgt tttacctgtt
     4321 ccaccttttt caatgtttcc agtaatgatt gacaacttct tttccatgat taaacctccc
     4381 ttattaatct tttgtaactc cattatacac tataaacacg tttatgtata tatttatata
     4441 cataaacgtg tttatattta tatttgcttt tatattctac aacataaaca tatatatgta
     4501 tatgtttatg tttatgtata tattcaaata tataactatt gcatttgtca tacaatcgta
     4561 gtacaatgta attgaaaagg agttgataaa ctatgccagt aataagtatt cgtgtatcta
     4621 aagaagaaaa agattttctt gaaaaagttg ctgaattcaa cggagataac atttctgaat
     4681 ttatgcgtag taattccctg caaacagcag aaacgttagt agactttgcc acctacaagc
     4741 aactaatgaa agagcatgag gaaaacgatc aaagtatttc ccacgaagat atgctaaaag
     4801 agttaggctt ataagatgac ttaccaagta agatatgaac gtgaagctca aaaagcgcta
     4861 aaaaaaatgg ataagtttca ggcaaaggtt atcctaaact ggatcgagaa aaatcttgta
     4921 ggcactgatg atccaagaaa gcatggtaaa ggtctaacag ctaataaatc aggctactgg
     4981 cgatataggg taggtgcata ccgtataata gctgatatta gcgagcatga agtgacaatt
     5041 cttatattgt ctattggtca cagaagtgaa gtttataagt aacagacaag ctgataaggc
     5101 ttgtctgttt ttttgttaac ccaacaaaat cactgtttac gatcccactt tacctaaaaa
     5161 agaacaatat cctcttgata ttatacacac cagtgtgtat aatataagtg taaacaagct
     5221 aattaacttt caaaaggagc gattttttat gggatggtat ggtgattatt catcagcaaa
     5281 tgaagtgatc gaagaaaaga aagggtacgc caaaaatggt ttggaaatcg taggtaccaa
     5341 agtaacgaag acctatggaa ttatcctttt caaagatgca gaaggaacct acgatattga
     5401 cagcttcatc tttcgagatg aaatgtacaa accaatctac ttcttaaatg acagtcctgc
     5461 ttactttaag aaaattccga aaaaatggtt gaaattcttt gtcggtgatc aagaaaaaat
     5521 ggttaagcta caactagata gaattaaaga agaaagcaat aaaccaaaac ttgatgactt
     5581 attagttgca ggtaaaactt actcaatctg ggatggatcg tatgtcgctg tctatcagta
     5641 taaactcaaa cgtagcttta tttttcgact acaagatgga tcactcactc ggttcactcg
     5701 actatcagca ggagatgtta aggaagtaac ctaaaactta aatacataaa cataaacgtg
     5761 tatacgttt
//
LOCUS       DACGLH010000129         5695 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.136, whole genome shotgun sequence.
ACCESSION   DACGLH010000129 DACGLH010000000
VERSION     DACGLH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.136"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(45..2969)
                     /locus_tag="IXT21_002823"
     CDS             complement(45..2969)
                     /locus_tag="IXT21_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ4870173.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3115..3690
                     /locus_tag="IXT21_002824"
     CDS             3115..3690
                     /locus_tag="IXT21_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4870174.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3904..4599
                     /gene="vanR"
                     /locus_tag="IXT21_002825"
     CDS             3904..4599
                     /gene="vanR"
                     /locus_tag="IXT21_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ4870175.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4577..>5695
                     /locus_tag="IXT21_002826"
     CDS             4577..>5695
                     /locus_tag="IXT21_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4870176.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELP"
BASE COUNT         1755 a         1078 c         1077 g         1785 t
ORIGIN      
        1 taaaatactt tgttttttta cctgtctact tgacaggggg cagttcactc tgagttaaaa
       61 tggtattctc ctagtaaatt aatatgttcc caacctaagg gcgacatatg gtgtaacaaa
      121 tcttcattaa agctacctgt ccgtttttta tattcaactg ctgttgttag gtggagagta
      181 ttccaaatac ttatagcatt gataattatg tttaaagcac tggctctttg caattgatgc
      241 tgtatggtgc gttctctaag ctcaccttgt tttccgaaga aaatagctct tgccaatcca
      301 ttcatggctt ctcctttatt caatcctctt tgtatttttc ttcttaatga ttcatccgat
      361 atataattca aaataaagat cgttttttct attcggccca tctcacgtaa ggctgtagct
      421 aagctgtttt gtcttgaata ggaacctagc ttccccataa taagggatgc tgaaactgtt
      481 ccctccctta tagaatgagc taatcgcaaa acatcctcat aattttcttt aatgaccttt
      541 gtatttattt gtccacgtaa aatggcttct agttttggat actcacttgc tttatctatc
      601 gtaaataatt ttgagtccga taaatccctt attcttgggg caaatttaaa tcctaataaa
      661 tgagtcagtc cgaatatttg gtcagtgtaa ccggcagtgt ctgtataatg ttcctctatg
      721 tttagatccg tctcatgatg taacaaacca tccaaaacat gaatcgcatc tcttgaatta
      781 gtatgaataa tctttgtgta gtaagaagag aattgatcac ttgtaaatcg gtagatggtg
      841 gctccttttc cagttccata atgtggattt gcatctgcat gtagtgatga aacacctagc
      901 tgcattctca taccatctga cgaagatgtt gtaccgtcgc cccaatagaa aggcaattgt
      961 aatttatgat gaaagtttac taatatggct tgggctttat tcatggcatc ttcatacatg
     1021 cgccattgag atacattggc tagttgctta tatgtaagtc cgggtgtggc ttcggccatc
     1081 ttgctcaagc caatattcat tcccattcct aaaagggcag ccatgataat gattgtttct
     1141 tccttatctg gttttcgatt attggaagca tgagtgaatt gctcatgaaa tcctgttata
     1201 tgggccacat ccatgagtaa atcagttaat tttattcttg gtagcatctg ataaaggctt
     1261 gcactaaatt tttttgcttc ttctggaaca tctttttcta agcgtgcaag tgatagcttt
     1321 cctttttcaa gagaaacccc atctaactta ttggaattgg cagctaacca ctttaacctt
     1381 tcattaaagc tgctggttct ctccgttata taatcttcga atgataaact aactgataat
     1441 ctcgtattcc ccttcgattg attccatgta tcttccgaaa acaaatattc ctcaaaatcc
     1501 ctatattgtc tgctgccaac aatggaaaca tctcctgccc gaacatgctc ccgaagttct
     1561 gttaaaacag ccatttcata gtaatgacga ttaattgttg taccatcatc ctcgtataaa
     1621 tgtcttttcc atcgttttga aataaaatcc acaggtgagt catcaggcac ttttcgcttt
     1681 ccagattcgt tcattcctcg gataatctca acagcttgta aaagtggctc atttgccttt
     1741 gtagaatgaa attccaatac tcttaatagc gttggcgtat attttcttag tgaataaaac
     1801 cgtttttgca gtaagtctaa ataatcatag tcggcaggac gtgcaagttc ctgagcctct
     1861 tctactgaag agacaaaggt attccattca ataaccgatt ctaaaacctt aaaaacgtct
     1921 aatttttcct ctcttgcttt aattaatgct tgtccgatgt tcgtaaagtg tataactttc
     1981 tcatttagct ttttaccgtt ttgtttctgg atttcctctt gagccttacg accttttgat
     2041 aacaaactaa gtatttgcct atcatgaatt tcaaacgctt tatccgttag ctcctgagta
     2101 agttgtaata aatagatggt taatatcgaa taacgtttat tttcttgaaa gtcacggaat
     2161 gcatacggct cgtatcttga gcctaagcga gacagctgca acaggcggtt acggtgcaaa
     2221 tgactaattt gcactgtttc taaatccatt cctcgtatgt attcgagtcg ttctattatt
     2281 tttagaaaag tttcgggtga aggatgaccc ggtggctctt ttaaccaacc caatatcgtt
     2341 ttattggatt cggatggatg ctgcgaggta ataatccctt caagcttttc tttttgctca
     2401 tttgttagag atttactaac cgtattaaat agcttctttt cagccattgc ccttgcttcc
     2461 cacaccattc tttcaagtgt agtgatagca ggcagtataa ttttgttttt tcttagaaaa
     2521 tctatgcatt catgcagtag atgaatggca tcaccatttt ccaaagctaa ttgatgaagg
     2581 tacttaaatg tcattcgata ttcactcagg gtaaaagtta caaagtcgta ttcacttcga
     2641 atttctttca aatgatccca aagtgtattt tccctttgag gataatgatc aagcgaggat
     2701 ggactaacac caatctgttt cgatatatat tgtatgaccg aatctgggat gcttttgata
     2761 tgagtgtatg gccaaccggg ataccgaaga acagctaatt gaacagcaaa tcctaaacgg
     2821 ttttcttccc tccttcgctt attaactatt tctaaatccc gtttggaaaa agtgaagtag
     2881 gtccccagta tccattcatc ttcagggatt tgcataaaag cctgtctctg ttccggtgta
     2941 agcaattctc tacctctcgc aattttcatt cagtatcatt ccatttctgt attttcaatt
     3001 tattagttca attatatatc aatagagtgt actctattga tacaaatgta gtagactgat
     3061 aaaatcatag ttaagagcgt ctcataagac ttgtctcaaa aatgaggtga tattttgcgg
     3121 aaaatcggtt atattcgtgt cagttcgact aaccagaatc cttcaagaca atttcagcag
     3181 ttgaacgaga tcggaatgga tattatatat gaagagaaag tttcaggagc aacaaaggat
     3241 cgcgagcaac ttcaaaaagt gttagacgat ttacaggaag atgacatcat ttatgttaca
     3301 gacttaactc gaatcactcg tagtacacaa gatctatttg aattaatcga taacatacga
     3361 gataaaaagg caagtttaaa atcactaaaa gatacatggc ttgatttatc agaagataat
     3421 ccatacagcc aattcttaat tactgtaatg gctggtgtta accaattaga gcgagatctt
     3481 attcggatga gacaacgtga agggattgaa ttggctaaga aagaaggaaa gtttaaaggt
     3541 cgattaaaga agtatcataa aaatcacgca ggaatgaatt atgcggtaaa gctatataaa
     3601 gaaggaaata tgactgtaaa tcaaatttgt gaaattacta atgtatctag ggcttcatta
     3661 tacaggaaat tatcagaagt gaataattag ccattctgta ttccgctaat gggcaatatt
     3721 tttaaagaag aaaaggaaac tataaaatat taacagcctc ctagcgatgc cgaaaagccc
     3781 tttgataaaa aaagaatcat catcttaaga aattcttagt catttattat gtaaatgctt
     3841 ataaattcgg ccctataatc tgataaatta ttaagggcaa acttatgtga aagggtgata
     3901 actatgagcg ataaaatact tattgtggat gatgaacatg aaattgccga tttggttgaa
     3961 ttatacttaa aaaacgagaa ttatacggtt ttcaaatact ataccgccaa agaagcattg
     4021 gaatgtatag acaagtctga gattgacctt gccatattgg acatcatgct tcccggcaca
     4081 agcggcctta ctatctgtca aaaaataagg gacaagcaca cctatccgat tatcatgctg
     4141 accgggaaag atacagaggt agataaaatt acagggttaa caatcggcgc ggatgattat
     4201 ataacgaagc cctttcgccc actggagtta attgctcggg taaaggccca gttgcgccga
     4261 tacaaaaaat tcagtggagt aaaggagcag aacgaaaatg ttatcgtcca ctccggcctt
     4321 gtcattaatg ttaacaccca tgagtgttat ctgaacgaga agcagttatc ccttactccc
     4381 accgagtttt caatactgcg aatcctctgt gaaaacaagg ggaatgtggt tagctccgag
     4441 ctgctatttc atgagatatg gggcgacgaa tatttcagca agagcaacaa caccatcacc
     4501 gtgcatatcc ggcatttgcg cgaaaaaatg aacgacacca ttgataatcc gaaatatata
     4561 aaaacggtat ggggggttgg ttataaaatt gaaaaataaa aaaaacgact attccaaact
     4621 agaacgaaaa ctttacatgt atatcgttgc aattgttgtg gtagcaattg tattcgtgtt
     4681 gtatattcgt tcaatgatcc gagggaaact tggggattgg atcttaagta ttttggaaaa
     4741 caaatatgac ttaaatcacc tggacgcgat gaaattatat caatattcca tacggaacaa
     4801 tatagatatc tttatttatg tggcgattgt cattagtatt cttattctat gtcgcgtcat
     4861 gctttcaaaa ttcgcaaaat actttgacga gataaatacc ggcattgatg tacttattca
     4921 gaacgaagat aaacaaattg agctttctgc ggaaatggat gttatggaac aaaagctcaa
     4981 cacattaaaa cggactctgg aaaagcgaga gcaggatgca aagctggccg aacaaagaaa
     5041 aaatgacgtt gttatgtact tggcgcacga tattaaaacg ccccttacat ccattatcgg
     5101 ttatttgagc ctgcttgacg aggctccaga catgccggta gatcaaaagg caaagtatgt
     5161 gcatatcacg ttggacaaag cgtatcgact cgaacagcta atcgacgagt tttttgagat
     5221 tacacggtat aacctacaaa cgataacgct aacaaaaacg cacatagacc tatactatat
     5281 gctggtgcag atgaccgatg aattttatcc tcagctttcc gcacatggaa aacaggcggt
     5341 tattcacgcc cccgaggatc tgaccgtgtc cggcgaccct gataaactcg cgagagtctt
     5401 taacaacatt ttgaaaaacg ccgctgcata cagtgaggat aacagcatca ttgacattac
     5461 cgcgggcctc tccggggatg tggtgtcaat cgaattcaag aacactggaa gcatcccaaa
     5521 agataagcta gctgccatat ttgaaaagtt ctataggctg gacaatgctc gttcttccga
     5581 tacgggtggc gcgggacttg gattggcgat tgcaaaagaa attattgttc agcatggagg
     5641 gcagatttac gcggaaagca atgataacta tacgacgttt agggtagagc ttcca
//
LOCUS       DACGLH010000130         5680 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.137, whole genome shotgun sequence.
ACCESSION   DACGLH010000130 DACGLH010000000
VERSION     DACGLH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.137"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            133..1287
                     /locus_tag="IXT21_002827"
     CDS             133..1287
                     /locus_tag="IXT21_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4870177.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQ
                     REMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1385..2758
                     /gene="tet(L)"
                     /locus_tag="IXT21_002828"
     CDS             1385..2758
                     /gene="tet(L)"
                     /locus_tag="IXT21_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ4870178.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3322..4584
                     /locus_tag="IXT21_002829"
     CDS             3322..4584
                     /locus_tag="IXT21_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ4870179.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            4829..>5680
                     /locus_tag="IXT21_002830"
     CDS             4829..>5680
                     /locus_tag="IXT21_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ4870180.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1953 a          805 c         1167 g         1755 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
      901 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     1021 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     1081 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     1141 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     1201 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1261 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1321 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt
     1381 aattatgaag tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg
     1441 cgttttatct ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat
     1501 tgcaaatgat tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt
     1561 aaccttttcc attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag
     1621 gttactccta tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca
     1681 ttctttcttt tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt
     1741 tccagcactc gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc
     1801 atttggtctt attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg
     1861 aatgatagcc cattatattc attggtccta tcttctactc attcctatga taacaattat
     1921 cactgttccg tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga
     1981 tatcaaagga attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc
     2041 atatagcatt tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat
     2101 caggaaagta acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat
     2161 tggagttctt tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc
     2221 ttatatgatg aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt
     2281 ccctggaaca atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag
     2341 aggtccttta tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc
     2401 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
     2461 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
     2521 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
     2581 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
     2641 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
     2701 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
     2761 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
     2821 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
     2881 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
     2941 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
     3001 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
     3061 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
     3121 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
     3181 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
     3241 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
     3301 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
     3361 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     3421 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     3481 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     3541 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     3601 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     3661 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     3721 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     3781 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     3841 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     3901 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     3961 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     4021 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     4081 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     4141 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     4201 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     4261 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     4321 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     4381 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     4441 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     4501 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     4561 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     4621 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     4681 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     4741 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     4801 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     4861 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     4921 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     4981 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     5041 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     5101 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     5161 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     5221 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     5281 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     5341 ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac
     5401 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     5461 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     5521 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     5581 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     5641 gttaatctcc tatggtggtt tgttaaaaga aatacataaa
//
LOCUS       DACGLH010000131         5212 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.138, whole genome shotgun sequence.
ACCESSION   DACGLH010000131 DACGLH010000000
VERSION     DACGLH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.138"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            604..954
                     /locus_tag="IXT21_002831"
                     /pseudo
     CDS             604..954
                     /locus_tag="IXT21_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            948..1370
                     /locus_tag="IXT21_002832"
     CDS             948..1370
                     /locus_tag="IXT21_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870181.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="IXT21_002833"
     CDS             2540..3508
                     /locus_tag="IXT21_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ4870182.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="IXT21_002834"
     CDS             3525..4049
                     /locus_tag="IXT21_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ4870183.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="IXT21_002835"
     CDS             4425..4706
                     /locus_tag="IXT21_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870184.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4708..5124
                     /locus_tag="IXT21_002836"
     CDS             4708..5124
                     /locus_tag="IXT21_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870185.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1969 a          894 c          867 g         1482 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc
      301 gaaaaatcaa gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaaataaact gcttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DACGLH010000132         5200 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.139, whole genome shotgun sequence.
ACCESSION   DACGLH010000132 DACGLH010000000
VERSION     DACGLH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.139"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..293)
                     /locus_tag="IXT21_002837"
     CDS             complement(<1..293)
                     /locus_tag="IXT21_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4870186.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRW"
     gene            complement(265..1464)
                     /locus_tag="IXT21_002838"
     CDS             complement(265..1464)
                     /locus_tag="IXT21_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ4870187.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1594..2970)
                     /locus_tag="IXT21_002839"
     CDS             complement(1594..2970)
                     /locus_tag="IXT21_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4870188.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2970..3626)
                     /locus_tag="IXT21_002840"
     CDS             complement(2970..3626)
                     /locus_tag="IXT21_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4870189.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3636..4913)
                     /locus_tag="IXT21_002841"
     CDS             complement(3636..4913)
                     /locus_tag="IXT21_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4870190.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4932..5087)
                     /locus_tag="IXT21_002842"
                     /pseudo
     CDS             complement(4932..5087)
                     /locus_tag="IXT21_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT         1647 a          948 c          656 g         1949 t
ORIGIN      
        1 aaccagcggt aagcgacatt ggtatcgact tccttaatgg tttgtcgcat ggaacggata
       61 ccgaataaat actgaataaa caccaatttg atcagaatca ctggatcgat acttgggcga
      121 cctaaatcgg agtaagtggc ttcgacgatt ggataaataa attcaaaatc aagcgcggca
      181 tcaatctttc gaacgagatg atcttgagga actagatcat caatcgtaag catcgcaaat
      241 tgatttctac cattcgtatt gtttttagac atcatagcta tcactccttg cattcgtttt
      301 cataaatcta ttataaaaga aaagactgta gacatcatcc ataaaatgga ctttgtctac
      361 agtctgcgca ttctcattag agaacactcc cttttttgtg acttttctga agctagagtg
      421 aatactttca actgcattag tagtgtacat gatttttcta atggcgctgc cataatcaaa
      481 gagttgttca acatgagaaa agtttcgttt gcatacatct actgcaccag aataatgaga
      541 ccaccgattt tgaaaactgt cgaaggcagc gtgtgcagcg tttaaagaag aagcactgta
      601 gaattttttc atgtctcgac agacttcctt ataatcctta cttgggaggt atcgtaacgc
      661 atttcgaaca agatgaacaa tacagcgctg cacaatgaca gaaggaaaaa ttgcttttgc
      721 gccttctttg agaccggaaa caccgtccat cgaaatgaaa aaaacatctt ctacaccacg
      781 tactttcagt tcatcaaata cttgcatcca gcgattttta gattctgttt gattgagcca
      841 taggcctaaa atctccttgt ttcctttaag atcatagcca agaatggtgt acacagcata
      901 ttctttagct tcatagtttt cgcgtaaagt gacgtacata caatcaacga atagaaatgc
      961 ataacacttg gctaatggcc gagcttgcca ttcttccaat tcaggaagga cggcatccgt
     1021 gatatcagaa atcatttcgt gggaaatatc aaagccgtaa atatcttcaa cggttgcggc
     1081 gatatcgcat tgactcattc ctcgtgcata catggaaaga accttacctt cgatgtcgga
     1141 gacatctcgt tttctattag gaatcaactc aggttcaaaa gaagcctccc ggtctctggg
     1201 aacatcaatg tttactttac caaagctcgt tttaagcgtc ttattgccat aaccgtttcg
     1261 tcgattgtca tgtttctttg gctctttaga atgggcgtca taccctaaat ggttattcaa
     1321 ttctccttga agcatttttt caaataaggg accaaacaca tcttttaaaa catcttgcat
     1381 atcctctaca gattcgggtt gataagtatt caaaatggat tcagctaact tctcagcatc
     1441 aggatttctt ttctttctag ccatcgtgta atcacctctt gatcttattt tagaaaaata
     1501 aaaaccgtga agcaaccacc cgcctaggat tgatggttac ttacacggtt tatattacac
     1561 tctcattttt ggtgctaaaa agcaccgaaa attttaatta ttgtcaatta ataattcttt
     1621 tggattcctt cttaatatat tgaatgaaga aataatcaat gatacaagaa gtactaaact
     1681 cataaatata actacataaa tcatgaattt aggtaataca ttgatatcta aacttgataa
     1741 tgtcttgtta aatccttcag cttctgcccc accacctaat tggctagatg cagattgttt
     1801 agctatttgt ttagctatat caccagtaac cttattcaat atattattcc caagcttgtc
     1861 agctgtatat tgagctaaga aataggaacc aacgaatgcg gggattgata taaataccat
     1921 ctcaattagg aattgaccaa aaatttctaa ttttgatata cctaatgata gtaatactgc
     1981 tatttctttc tttctagcat tcatccataa gaataataat aatgaaacta caactcctgc
     2041 aaatattaat gagccaaaaa ataatttatt tgaaattgag taaatacctg atatagattg
     2101 ttgtaatgca gggtaatttg atgaactttt aatcaaatta tattctctcc aatttatatc
     2161 taatttccca agatctttta ttacactatc aagattctta tcacctttta caaagaatgt
     2221 tgcatcttgg tatacagctg tatcttcggt attaccataa actttagcag ctgtatgaat
     2281 atcagtgatt aatgtatttt cgtatagttc ttgtgctgca cttactccac cgctattatg
     2341 cccatcgaat agacctttaa tttctacttc tactgtttca tctgcacctt tctcattatc
     2401 agcatcaaat aaattagatt ttatttttat cttatctcca actttaaggt tatttttttc
     2461 agctaaatct ttatgcatta agattttatt tttatcttca ttttctaaat gctttccttc
     2521 tactaattta tatgcttctg atacaaattt tgtttctttt gttgagtcgt taaccccagt
     2581 taacataact gttcttttaa aattctttgc tctttcaggt gattgattcg caagagtctc
     2641 ttgagtttca ataatatcat aatcaactaa atctgcaaca ctgtttattc tttttacata
     2701 ggagtctata ctatctgttt gagatatctt tttaatatct tcacctttta catttccccc
     2761 acctctaggt gttcctggat ttacttgtct atttatctcc atagaaaaac tatttgttat
     2821 attagcaaat gtttcttttg aagctctgtc cgtagcatct ttaatagata aactaatgag
     2881 acttaaagtt gacatagata aaataactaa tatgataatt aatgatttta aactttttct
     2941 agttacataa gcaaatgcat tttttatcat tattcaacct ccaaattcat tttatttact
     3001 tttttcaact ttttaccact tagttctaaa atgatatccg cagaatctgc tacttcctta
     3061 ctatgtgtta caactattac acatttattt ctatctttag ctaatgtctt taatatatta
     3121 attatttctc cagcagtaac actgtctaga ttaccagtag gctcatcagc tagtattatt
     3181 ggagcatctg ataccaatgc tctagcaata gctactcttt gttgctgtcc accagataat
     3241 ttcataacat ttctttttat ttgtttttta tctaaaccta gttcaaacaa gatactttca
     3301 tctgctgatt tatttactaa tctaatattt tcaattggtg ataaataatc tattaaatta
     3361 tagttttgaa atactaaaga tatattattt tttctatgat tactatatcc tttattttgt
     3421 atatcttcat tcttaaacaa tatttttcct gtttgaggtt tatcaagtcc agcaagtaag
     3481 gaaagaagtg tagattttcc tgttcctgac tttcctacta tcgcataaaa cttcccaagt
     3541 tcaaattttt gatttactcc tgacaaaact ttttctttag aatttgcata actatatgct
     3601 acattcttta tttctaatat atccattatt ttctcctaac ttatttttga taagatttct
     3661 ttaggcttct tgattaacat taatgaagaa gcaaatacaa ctgataaaac aataatactt
     3721 attaatatta aataactttg tccaagtgtt gttatatttg acataaaact actattattt
     3781 attaaacttc caccggaaat cattgaatcc tctgagttaa tgaatccatc tacaattact
     3841 tttagtaata catttcctaa aaataaggaa gatattatac ttggtatcga tatgaatatt
     3901 aactcgaata taaattgcat tataatttgt atcttagatg ttccaataga taagaatata
     3961 cctatttcat aaattctttc ccttaaccat agaatcaaga ttaatgaaag aacaaccatc
     4021 ccacctaaca taatagaata agtcattact tttattatat attttattcc actcactgac
     4081 tctaaagact cttcaaacgc attagaatct ttttcaacaa aatactttga ctcatcaatt
     4141 ttaagctctt tcaatttgtt taaggctaag tctgtagatt ctgcactacc agaatacatt
     4201 aaaattttat ttgcaatttc attattttct gaattattta atatctcttg gcttgttgaa
     4261 taatctacaa acaccatatt ttcactaaaa tcagaagata atcctgtata tgtttcctgt
     4321 tttttaccag aaaagatccc tataatttta aatttatgac tttttatttt tccacttttt
     4381 tcaatatcta gtaattcaag atcaacttca tcacctaatt ttagattgtt ttgtttagca
     4441 aattcttcat gaacaataat tgaattctta tcattttctt ctatattttt accttcttta
     4501 attgtaaata ctccactact aaataaaata tttcttttag tattatttgt agcttcgagt
     4561 gaaacaacat tcttaaattc gtcagataaa tcttctctat ttattctttg ttctccgcta
     4621 actactttag catcttttag ttttgctaat ccatcatatt gaattatttt ttcttcaatt
     4681 tcttttaatt tttcaatatc tttaaattga ttaacattaa aatatttacc atcttttttt
     4741 gttattgata ttgaagaatt agaactttca tataaagcct tttctatttc attgcttgat
     4801 ttcattattg ttaaacaaga atacaagcaa gaaagaacta tagttaatat gataaaaatt
     4861 attaaagttc tatttttctt tcttgtaata tatgctatag cattttttat cactttccta
     4921 actactcctc tctattttct tactccataa ctaattgcat ccactggaca agcattttta
     4981 catcgtccac atctgataca ctccagatga ttgcaattct caactgaatc tttattcatt
     5041 tggcaaactt ttttacattt tccacaatta atacacttat ccttatcctg aatcggctac
     5101 actagactag acagaaaaaa taaggtgtgt agaataataa aaaacacact ggaggatttt
     5161 tcatgccaag acgtcaacga agaacctatt caaaagaatt
//
LOCUS       DACGLH010000133         5072 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.140, whole genome shotgun sequence.
ACCESSION   DACGLH010000133 DACGLH010000000
VERSION     DACGLH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.140"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            73..1011
                     /locus_tag="IXT21_002843"
     CDS             73..1011
                     /locus_tag="IXT21_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ4870191.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            1026..1856
                     /locus_tag="IXT21_002844"
     CDS             1026..1856
                     /locus_tag="IXT21_002844"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ4870192.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            1900..2925
                     /locus_tag="IXT21_002845"
     CDS             1900..2925
                     /locus_tag="IXT21_002845"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ4870193.1"
                     /translation="MNYRVAVLGPGSWGTDLAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            2940..3878
                     /gene="galU"
                     /locus_tag="IXT21_002846"
     CDS             2940..3878
                     /gene="galU"
                     /locus_tag="IXT21_002846"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ4870194.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            4003..4443
                     /locus_tag="IXT21_002847"
     CDS             4003..4443
                     /locus_tag="IXT21_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ4870195.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            4446..5048
                     /locus_tag="IXT21_002848"
     CDS             4446..5048
                     /locus_tag="IXT21_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4870196.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         1740 a          869 c         1119 g         1344 t
ORIGIN      
        1 taattggtag tacttttggc tagacaatga taaaatgata gagaacactc taatagaaat
       61 gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa attatctttg
      121 gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg ggacatctca
      181 cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg tttacagcta
      241 tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg tttgatcatc
      301 atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg attaccagct
      361 ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg ttctcccatc
      421 tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct tgcccctcga
      481 acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat tcaaggtgac
      541 agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggcca tcgattgatc
      601 gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg agaaccgcct
      661 aaaatcctgg aacatctgat tgaaatccga ggaatcggga tcattgatgt aatgaatctt
      721 tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta tctggaagca
      781 tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt tgaaatcgcg
      841 gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt tgcaatcatc
      901 attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc aacgaaaaca
      961 tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta gcaaggagtg
     1021 acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt cctatctact
     1081 ggtatgcatt gatcatcgta tcaggtatca ttatcgctat gtggcttagc agcagagagg
     1141 ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg ggattgccta
     1201 tctccattat tggcgctcgc ttatattatg ttttatttga tcttccccag tatatagctg
     1261 atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc ggactgattg
     1321 caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg tggacttttt
     1381 tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg ggaaatttca
     1441 tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt ctgcatttac
     1501 cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca acatttcttt
     1561 atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga aagaaaccac
     1621 accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt ttcgggcgtt
     1681 tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc cgtatttctc
     1741 aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg cgcagaaaaa
     1801 aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt atctgatttt
     1861 tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag agtagcggtt
     1921 ttaggaccag gctcttgggg tactgatttg gcacaagtcc tcgccgaaaa cggacatgac
     1981 gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca acacacgaat
     2041 caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac caatatggaa
     2101 gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc aatacgttct
     2161 gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca tgcaagtaaa
     2221 ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga aatcccagca
     2281 gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga agtagcaaaa
     2341 catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa atatgtacaa
     2401 gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat cggtgtagag
     2461 atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca cggcttaggg
     2521 tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat cagccgttta
     2581 ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggaat aggcgatcta
     2641 gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt gttaggacaa
     2701 gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg tgtgaatact
     2761 acccaagcag cgatggaact agcccaaatg atggaaatcg aaatgcccat cactcaaaca
     2821 atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga aatcatgtta
     2881 cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt tttgaaggag cgaatgtata
     2941 tgaaagttaa aaaagcagtt attccagcag caggattagg aacaagattt ttaccagcaa
     3001 caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc cagttcatcg
     3061 ttgaagaagc gttgaaatca ggtatcgaag acatcttgat cgttacagga aaagctaaac
     3121 gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta agtgaaaaag
     3181 gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg catttcatcc
     3241 gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc tttattggaa
     3301 atgaaccatt tgtcgttatg ctaggcgatg atataatgga agacgaagta ccattgacac
     3361 agcaattaat cgatgattac gaacgtactc atgcttctac gatcgccgtt atgcaagttc
     3421 ctcatgatga aacttcaaaa tacggaatta tcaatccagg agaagtattg gaagatgggt
     3481 tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca agtgatttag
     3541 ctattattgg acgttactta ttgactccag aaatttttga tgtattgtcc gagcaacaac
     3601 caggagccgg aaatgaaatc caactaactg acgcaatcga tacattgaat aaaacacaac
     3661 gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt cggtgacaaa tttggtttta
     3721 tgaaaacaag tattgaatat ggtctaacac atccagaagt aggtgcacct ttacgtgagt
     3781 acatcctgtc tttaggtaaa acattagaag cagaaggaag caagatcgat cataatccaa
     3841 aaggaataac agaaatcaaa gaagaaaaga aaaaataacc cttttttcta acgagcttca
     3901 gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa aacacgaaaa
     3961 tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg aggagaaatt
     4021 gcaggattga tcgcagcaat cgcctttgcg atacttgtcg tttttatcgt tcgtgtgttg
     4081 ttgcaagttt ctaaaacttt agaaaaagta gatcagactg tagcagaagc aaatacgacg
     4141 atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac aagtagaagg cttactggtc
     4201 aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg caacgatcga tccattgttc
     4261 acagcagttg ctgatttaag tgaaagtgta tcagaactta atcattctag taaaaacatc
     4321 gcgacgaaag tcggcggaat cggtaaaacg acagctaaag cgacggttgc cggaaaagtc
     4381 ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac aggaggaaaa
     4441 taaaaatggc taaaaaaggt ggatttttat taggtgcagt agtcggaggc acagccgcag
     4501 caatcgccgc gttattgtta gcaccaaaat ctggtaaaga acttcgggat gaattagctg
     4561 cccaagcgga tgatttgatg gatatggctt cagattacac agatatggcg aaagaaaaat
     4621 cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga tctttctcaa caagcaggtg
     4681 atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc agatgaagca ggaagtgaat
     4741 ctgacgatat cttgttaaat cttaaagaac aatcagctga cttatctaat cgtttcaaaa
     4801 aaacggcaga agatatgaaa aaacctgcag atgaattagg aagtattgct aaagatactt
     4861 cagaagatat catgattgat gtaaaagacg ctgctggtaa agcaggagat actgtcaatg
     4921 aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa agaaatgatg aaaatggcaa
     4981 aagaagatgc gaaagaagct aaaaaagaaa taaaagatac aatgacagat tcatctacaa
     5041 atcactaaaa tcacaaattt atagaataaa aa
//
LOCUS       DACGLH010000134         5023 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.141, whole genome shotgun sequence.
ACCESSION   DACGLH010000134 DACGLH010000000
VERSION     DACGLH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.141"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            282..521
                     /locus_tag="IXT21_002849"
     CDS             282..521
                     /locus_tag="IXT21_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870197.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IXT21_002850"
     CDS             complement(883..1143)
                     /locus_tag="IXT21_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870198.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IXT21_002851"
     CDS             complement(1328..1825)
                     /locus_tag="IXT21_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ4870199.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IXT21_002852"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IXT21_002852"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ4870200.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IXT21_002853"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IXT21_002853"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ4870201.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..>5023)
                     /locus_tag="IXT21_002854"
     CDS             complement(4547..>5023)
                     /locus_tag="IXT21_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4870202.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1528 a         1042 c          771 g         1682 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gca
//
LOCUS       DACGLH010000135         5014 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.142, whole genome shotgun sequence.
ACCESSION   DACGLH010000135 DACGLH010000000
VERSION     DACGLH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.142"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            192..290
                     /locus_tag="IXT21_002855"
                     /pseudo
     CDS             192..290
                     /locus_tag="IXT21_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(513..743)
                     /locus_tag="IXT21_002856"
     CDS             complement(513..743)
                     /locus_tag="IXT21_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ4870203.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(973..1791)
                     /locus_tag="IXT21_002857"
     CDS             complement(973..1791)
                     /locus_tag="IXT21_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4870204.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1952..2641
                     /locus_tag="IXT21_002858"
     CDS             1952..2641
                     /locus_tag="IXT21_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ4870205.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2655..4157
                     /locus_tag="IXT21_002859"
     CDS             2655..4157
                     /locus_tag="IXT21_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4870206.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4170..4646
                     /locus_tag="IXT21_002860"
     CDS             4170..4646
                     /locus_tag="IXT21_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ4870207.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4743..4853)
                     /locus_tag="IXT21_002861"
                     /pseudo
     CDS             complement(4743..4853)
                     /locus_tag="IXT21_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1694 a          724 c          888 g         1708 t
ORIGIN      
        1 accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga
       61 cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt
      121 tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta
      181 gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga acacctttgg
      241 aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga caattaagag
      301 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
      361 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      421 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      481 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      541 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      601 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      661 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      721 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      781 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      841 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      901 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      961 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
     1021 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
     1081 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
     1141 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
     1201 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
     1261 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1321 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1381 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1441 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1501 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1561 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1621 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1681 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1741 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1801 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1861 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1921 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1981 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     2041 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     2101 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     2161 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     2221 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     2281 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2341 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2401 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2461 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2521 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2581 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2641 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2701 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2761 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2821 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2881 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2941 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     3001 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     3061 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     3121 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     3181 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     3241 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3301 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3361 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3421 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3481 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3541 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3601 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3661 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3721 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3781 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3841 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3901 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3961 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     4021 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     4081 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     4141 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     4201 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     4261 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4321 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4381 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4441 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4501 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4561 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4621 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4681 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4741 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4801 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4861 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4921 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4981 tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DACGLH010000136         4991 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.143, whole genome shotgun sequence.
ACCESSION   DACGLH010000136 DACGLH010000000
VERSION     DACGLH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.143"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..81
                     /locus_tag="IXT21_002862"
     CDS             <1..81
                     /locus_tag="IXT21_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ4870208.1"
                     /translation="MVNETARKAIDLHIENLRREIRRLEE"
     gene            84..383
                     /locus_tag="IXT21_002863"
     CDS             84..383
                     /locus_tag="IXT21_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870209.1"
                     /translation="MDKKATMKRIVELTKSESWQEDKEIVAEVQRIGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            383..700
                     /locus_tag="IXT21_002864"
     CDS             383..700
                     /locus_tag="IXT21_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ4870210.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYKE
                     DSK"
     gene            697..1113
                     /locus_tag="IXT21_002865"
     CDS             697..1113
                     /locus_tag="IXT21_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870211.1"
                     /translation="MIPKFRAWDKKTETIQEIESISFKEKKLVIDQKSVTWFNSDYIR
                     NFGEVELMQSTGLKDKNGVEIFEWDIVLVNVSNGFDHLVNEKTVVQESEFHSGLIYKS
                     LASGMEYRVFKHKKMDYEYEVIGNIYENSELLEEQR"
     gene            1110..1670
                     /locus_tag="IXT21_002866"
     CDS             1110..1670
                     /locus_tag="IXT21_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ4870212.1"
                     /translation="MNKQELINELKKISLEKQHKALTHPESKNYYEGMVMAYSQVVHM
                     VNTVLKLDEPQKPVVPKFVAEWFEENKDDLEFEIWSLCVNSYCSTTGQEMMNWIQQSE
                     NKPIETLIRMKEGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDTSCSLCAWEKSAYQ
                     LTEAEIKAIDERYWPFAVPVEEVAEG"
     gene            1837..2205
                     /locus_tag="IXT21_002867"
     CDS             1837..2205
                     /locus_tag="IXT21_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870213.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            2202..2507
                     /locus_tag="IXT21_002868"
     CDS             2202..2507
                     /locus_tag="IXT21_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870214.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            2531..2734
                     /locus_tag="IXT21_002869"
     CDS             2531..2734
                     /locus_tag="IXT21_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ4870215.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            2731..3048
                     /locus_tag="IXT21_002870"
     CDS             2731..3048
                     /locus_tag="IXT21_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870216.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDMEVINNDCKWE
                     EMR"
     gene            3385..3801
                     /locus_tag="IXT21_002871"
     CDS             3385..3801
                     /locus_tag="IXT21_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ4870217.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            4710..>4991
                     /locus_tag="IXT21_002872"
     CDS             4710..>4991
                     /locus_tag="IXT21_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ4870218.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSSKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTA"
BASE COUNT         1797 a          710 c         1181 g         1303 t
ORIGIN      
        1 atggtcaatg aaacggcgag aaaggcaatc gatttgcaca tagagaattt aagaagggaa
       61 attcgtagat tggaggaata aacgtggata agaaagcaac aatgaaacga attgttgaat
      121 taaccaagtc agaatcttgg caagaagaca aagaaatagt tgcagaagtc caaaggatcg
      181 gcaaatcaat gtggactgaa aagcccaaac ggagaacgcc aagaaaaatt gcaatctggc
      241 atggtgatcg aattctagta acaggtactg ctgaacagtt atctgaaatt actggattaa
      301 gcaaaaacat catctgggat agagctagga gcttatggat tgattcaaaa ggacgacagt
      361 ttaggtatgt ggaggagaaa taatggatct cattacacaa tatagtgata tcatcctcaa
      421 gaaaatcatg atgaagattc agaaagacaa aaaatcaaaa gaacgagcgg aattagttaa
      481 gttggaaatg gctgaaacag gagcaggagt gcgaagtagc aggcattgga aagcagcagc
      541 aaacattgaa ttttattaca acgaaattca aaaagggttc gatcagatgc gtgagctgga
      601 tcggcaaaca aattggagca agaaacttca tcaagatcgt ttcaaatttg tagagaaata
      661 taaagagata ttagacgaat acaaggagga cagcaaatga taccgaagtt tagagcgtgg
      721 gataaaaaaa cagaaactat acaagagata gaatcaatat ctttcaaaga aaaaaagcta
      781 gtgattgatc aaaaatctgt cacttggttc aactcggatt atattagaaa ctttggcgaa
      841 gttgaactca tgcaatccac aggactgaaa gataagaatg gtgtggagat atttgaatgg
      901 gatatagttc ttgtcaatgt aagtaatggt tttgatcatt tagttaatga aaaaacggtt
      961 gtacaagaat ccgagtttca ttctggattg atttataaat cattagctag tgggatggaa
     1021 tatagagtat tcaaacacaa aaagatggat tatgaatacg aagtcatcgg aaatatatac
     1081 gagaatagcg agttattgga ggaacagcga tgaataaaca ggaattgatt aatgaactaa
     1141 agaaaatttc actagaaaaa cagcacaaag ccctgacgca tccagaaagc aaaaattact
     1201 atgagggcat ggtgatggct tattctcaag tcgttcatat ggttaatacg gtgttaaaac
     1261 tagacgaacc gcagaaacca gttgtgccga agtttgtggc ggagtggttt gaggagaata
     1321 aggatgattt agagtttgaa atttggagct tgtgtgtaaa ttcttactgt tctactactg
     1381 gacaagagat gatgaattgg attcaacaat ctgaaaacaa accaattgaa acccttatcc
     1441 gcatgaaaga aggttacgag gtcgagaaag agccgttgta tgaagttatt attggtgact
     1501 tatatcttat caagaaattt aataacagaa atgatttctg ttttgatact agctgctcgc
     1561 tgtgtgcttg ggaaaaatct gcttatcagc taacagaagc ggaaatcaaa gcaattgatg
     1621 aaagatactg gccatttgct gtgccagtgg aagaggtggc ggaaggatga gcagacctct
     1681 aatgctccat ataccagacg gaacaaaggc aattgcaatt agcattgttg ctgaacaaaa
     1741 agatggcggt ttggcgttga aaacaaaagg tattgatacc aaacagattt tagaaggcag
     1801 agatgtcgag atagagatcg atgaggagga agcggaatga acgaggaaat aaaaaaatct
     1861 attcgttaca ttgatagagc gttatcgcaa ctcgaaccct tgcgaattga aagtcataaa
     1921 ggaatgagtg tctacccaaa tcagtatgac aaaatgaacg ctgatttacg taatgcaaag
     1981 ctggccttaa ttaacattaa aaataacaaa ggctgccagt attgcaaaca cagcgaaccg
     2041 ttgaatgata ccagcaactc taactttgca actgcaattg agtgtgagga aaatggatac
     2101 tacattgcaa ctgagtatga cgatggcgat tatgcagata atggctattc tgcttatatc
     2161 caatattgcc ctatgtgcgg tcgaaaattg gaggtagcgg aatgaatgtt caagtttatt
     2221 taaaaagaaa tttggccagg tttcctcgaa gctgggatac aaattcccac ccatccgagg
     2281 cagaagaaga gagctatcta atgtctgcta acatgataga aattacaaga gatgatgcgg
     2341 atgagtttga taaaaaaaga tcatggtact cggtttcgaa tccaatgtat acggctgtaa
     2401 tagaagatta tcatatatcc gacagattta ttctggttga tctagaaaga cctaagaaac
     2461 gaattaaaca gtatagacgt tgcgggtttt caacaaagaa atggtgaaaa caaaacagag
     2521 gtgactcaga atgaacaaca gacatcgcag aataacaaaa ctaagaaaac aggaactgaa
     2581 tgtactaaag acaaagtttg aaaaagaata cggagtttct gcagaagaaa catataaagt
     2641 ggcaagtcag tgtgttgctg atgcaagtga tgctattcgt aagtttggaa tttcgatatt
     2701 aagtgatgat cgtaaatggg aggaaaaaga atgaaactaa aagacggatt ttacgctagt
     2761 agccacggta tcggcggttt aatgctggat atgcagacaa agaaccctaa aacacgtaag
     2821 aaaccaaaat tcaaagtcgg tgacatggtt cgctgcgaag cagaagggtt catctatcca
     2881 tttcgtggat atgtagaaca tgtctataat cactcagcga tcattcgcat tgaaaacacg
     2941 atggaatgcg acaagttatt agcgaaaagc aaagagaatt tagcagtggt gagattggtg
     3001 gatatggagg ttataaacaa tgattgtaaa tgggaggaaa tgagatgaag gaattaattt
     3061 taaataaaga aaatattctt gacttattag attgcgtgaa cgcttggatt ctgctgtatg
     3121 gctatgaaaa catgcgtcct gaaatgaaag aattagcttc taaattatcg gaagcgattg
     3181 aagtttagat ttagcaaagg aggatacaga atgttagtaa aaattattgt aggagttatc
     3241 ttttggatta tgactttgat gctaggttac tttatcggaa aagaagaagg tcgtaaagaa
     3301 ggcaaataaa aaagccggat cgctccgact aacataataa aacagacaag tttattatat
     3361 cacataaagg agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca
     3421 agatgtaatg cgagagatgt gctgaagaac tttcggcgtt tggagcgtat ggcaggtcgc
     3481 tctttgatag atattaagtc gccgattata acggatatgc cgaaggcgcc gaagcacggc
     3541 aataaggcag aggacgcgat cattcagatg atggatatag aagcagagag agatgcgatt
     3601 ctagcggctt tgatggctct tagtctgatt agtcgtcaga tactctacta cagcttctgc
     3661 gacgtaaata agcattctaa ttatgaaata ggtcaattga tacgaggata cggagagaag
     3721 aatgtagaga agttgaaatc catcgcattg attgaatttg ctgaagcata taaaaaaggc
     3781 gtattaattc agtatcgttg attttgtagg gtttttgtag ggatagtgta gggtttttga
     3841 gcggtttaac gtgatattat ggtagtgtcg aaagattagg aaacaggact tcgacaaaat
     3901 aaaatgtaag ggaggaaatc tccctcatcg tttaattaag cttcgataga cagcagcgga
     3961 aatattaaga ataaggatgt gaattttaac tccttctaaa ttgttcttat tatctatcat
     4021 ccgttgctgt ctattgtcat tatgtcactg tggcggaaag ggtagacgct taaaaataag
     4081 gtcaatacgt cgagggatag ccttaacgtt ttatgatttg accatgcaat gttcgattca
     4141 ttgccagtga cttaaggaac ctacggaaac aattcatctt atcggatgcc gatgaattgg
     4201 ctgactagtc gggatgctac tagcagttag aaggcataaa atactagcgc agacgtgcgc
     4261 cactctcagg tgtaggttag gagagaaaca ttagttgggg ttattaggaa tacgataacc
     4321 tgcttgcgac aaagctttgt actgtcgcgt tggtcatgaa cagagacggt attctgtttc
     4381 agtattcgtt agcaaccgag ggatgtggca gtggtgaggt gcaggaagta tcagacttgt
     4441 ttgtgtgcaa gttactatta catattagat cactctttga gtggtctttt tattttgcac
     4501 aaaggaggta acaacaatgt atagaccaca atacttagaa cagaagtatg aagtaatcac
     4561 tgttcaaaat ggtaacagtg agatagtacg aaagtataga agaccaataa agagcgatac
     4621 atataaacga aaggaaaaca atgaagttat tccattgtat ggcaaaagaa tagctaagca
     4681 ttaaataaga ttgcgaaagg agacgggaca tgactgagga attctataga tggctattac
     4741 agttgataag agaagatcgt ttggttaagt tctatcagtc ttctaaatgg cgcaggctta
     4801 gagagaaagc gatgaaacga gatcactatg aatgccaaga gtgtagaaga ctaggtaagt
     4861 atcatagagt agagaacgtt catcatataa aggaagtcaa ggatagacct gacttagctt
     4921 tagatttaga taatcttatt tgtttatgtg ttgaacatca taatgaagtt catggcagat
     4981 atcttacagc a
//
LOCUS       DACGLH010000137         4938 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.144, whole genome shotgun sequence.
ACCESSION   DACGLH010000137 DACGLH010000000
VERSION     DACGLH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.144"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(207..1169)
                     /locus_tag="IXT21_002873"
     CDS             complement(207..1169)
                     /locus_tag="IXT21_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4870219.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IXT21_002874"
     CDS             complement(1171..2610)
                     /locus_tag="IXT21_002874"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ4870220.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELFLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IXT21_002875"
     CDS             2795..4741
                     /locus_tag="IXT21_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ4870221.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGVNQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1596 a          828 c          803 g         1711 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaaataac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagtaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttgtctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtctattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatgtatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaa
//
LOCUS       DACGLH010000138         4864 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.145, whole genome shotgun sequence.
ACCESSION   DACGLH010000138 DACGLH010000000
VERSION     DACGLH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.145"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            115..2112
                     /locus_tag="IXT21_002876"
     CDS             115..2112
                     /locus_tag="IXT21_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ4870222.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAAWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2298..3107)
                     /locus_tag="IXT21_002877"
     CDS             complement(2298..3107)
                     /locus_tag="IXT21_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4870223.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            3400..4836
                     /locus_tag="IXT21_002878"
     CDS             3400..4836
                     /locus_tag="IXT21_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ4870224.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         1508 a          882 c         1029 g         1445 t
ORIGIN      
        1 ttagaaaaaa taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat
       61 gttccttttc gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt
      121 gctagattgg gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct
      181 acttctacga ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga
      241 aaagctttag ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt
      301 acggctaaag atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta
      361 gatgaaaatc tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg
      421 aaagaagcca caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat
      481 agtgcgatgg tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa
      541 attgctacca tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa
      601 aactctggtg ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca
      661 ggagtttcga ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt
      721 attcttggca gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac
      781 ctagcaaaag gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat
      841 gaagatgtcc tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat
      901 gtttcgaatc aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg
      961 aatgcagaat tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc
     1021 aacaacggca aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca
     1081 ggagaagtgc tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag
     1141 aatgctttag gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact
     1201 ttgacagcag cctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg
     1261 attcgtctgc aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct
     1321 cttgatgcag tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta
     1381 aagatgttag gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct
     1441 aaagcttatc agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg
     1501 atcgatttgc cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc
     1561 aatgcggata atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga
     1621 cagtttgaca catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc
     1681 gggaaacgag tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac
     1741 ttgggtgaag tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca
     1801 gaaaatatgg cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac
     1861 acaacagcca aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca
     1921 gaaacggttg cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac
     1981 ggaccaacag acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa
     2041 caagaccaca taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc
     2101 atggcaaaat aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt
     2161 ttgtgaaaat agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt
     2221 ttgaacaccg tttactcggc aaaaaaggtt gtgacaaaaa ttgtcacaac ctcttttttg
     2281 tggaatctaa ttctctatta tagtttaagt agtttttcca gataaacagc taaaccgtct
     2341 tcctgattgc tcaaaggagt gacatcattt gcaatgcctt tgagttggtc agtaccgtta
     2401 gccatagcta ctccccaacc tgcataatcc aacaattcca aatcattatg ctcgtcacca
     2461 aaggcaatga cgtctttttg atcgatagac aggtagttag caatctcttg cacgcctttt
     2521 gctttttgga ttccttttga aacaatctct aagatggcag taggtccacc ccatgtccga
     2581 acatcgacat aactgccaaa ttgacgtgtt aattcagcag atacagctcc tgcaaaacgt
     2641 ttgtctgttc ggacaagcaa agatgtagga tttgtcgtca aatttttagg agaaagcaaa
     2701 ttcttctctc ctggacgaga agaagcaaaa attttttcat caaagaaatc aaaactgtca
     2761 ataaaaaaag tatcacgatt ttcagcagcg ataaaatcta aatttagctg ctttttttgc
     2821 gataagattt caaaagcgat ttcacgattg atcaacgttt ctctttcacc atcccagtgc
     2881 tgattaggga tgtgtactaa tgcgccgtta aaattaacca tcggtgttgt taattctaat
     2941 tgttggtaaa actgatggct cattcggaat gggcgtcctg tagcaatact gacatgatga
     3001 ccggcattga ttgctttttt tagagtctct tttgttttag atgtaatcat cgaatcttga
     3061 ttgagggtag ttccgtccag atcgatggca attaattttt tattcaataa gggtccttcc
     3121 ttcctcaact agcatttgtt ttagtctagc atattttagg taattaacaa gagcaatcag
     3181 ctattcctaa caaaaactac agttatggct atataaatag ctaaacataa gctaaacacg
     3241 aacgttaagt attattttcg ctttttttgt taaaaaaacg ttgaaaagcg aatgaaacgt
     3301 ctatataatt tgaaattgtt cgatggattt gccctatttt acttattttt tattattata
     3361 gttttccatc agaaaaaaat tcagggagtg gaacttttta tggaaaagtt tttcggttta
     3421 aagaaaaaca atacaaatgt atcaactgaa ataatggcgg gagtaacgac attctttgcc
     3481 atgagttata tcttattcgt aaatccgacg attttgtcag catcaggtat gcccttccaa
     3541 gcagtatttc tagcgacaat cattgcatcg attattggta cgctggtaat gggattgttc
     3601 gcaaatgtac catatgctca ggcacctggt atgggattga acgcattttt cacttttacg
     3661 gtcgtttttg ggcttggtta ttcttggcaa caagccttgg caatggtgtt tatttgcgga
     3721 ttaatcaata tttttattac tgtcacaaat attcgaaaaa tgattattcg ggcgattccc
     3781 gaaagtcttc aacatgcgat tggtggcggg atcggtattt tcgttgcgta tgtcggaatc
     3841 aaaaatgcag gattcttatc tttttcagca gaccagtcag ctatttcgag ctctgtcgtt
     3901 gaaggcggaa aagcgacaaa tgttacgata aatggtggaa tcgtccctgc gttagccaac
     3961 tttgacaacg ctcctatctt attggctgtg attggtttgg tactgacagc cattttagtt
     4021 gtaaaaaatg tccgtggggc tattcttatc ggtatcgtag cgaccacagt ccttggtatt
     4081 tttatgggtg ttgttgattt aagttccatt gattggcaaa cgaattcgtt aggtaattca
     4141 tttaaagaat taggaacaac tttcggtgca gcatttggtt ctgaaggcat gcaatcttta
     4201 ttcagtgatt cttccaagat tcctcaagta ttgatgacga tcatcgcatt cagtttatct
     4261 gatactttcg atacgatcgg aacatttatc ggtacaggac gccgcacagg tattttctca
     4321 aaagaagacg agatcgcgtt ggaagatggt cgtgggttca aaacaaaaat ggataaagca
     4381 ctatttgccg atgcgatcgc tacttctgtg ggggctatct ttggtacatc gaatacaaca
     4441 acttatgtcg aatctgcagc tgggatcggg gccggtggac gtactggttt gacttcagta
     4501 gtagtggcag tgctattcgc attaagtagt ttattctctc cactgatcgc cattgtacca
     4561 aatcaagcaa ctgcgccagc attgatttta gttggagtaa tgatgatggc ttcttttgca
     4621 gacatcaaat ggctagatat ggaagaagct ttaccggcct tctttgcatc gatctttatg
     4681 ggattgtgct atagcatttc ctatgggatt gcagcaggat ttattttcta tacaattgtg
     4741 aaagtaatca aaggaaaata caatcaagtt tctgttgctt tgtggatcgt tgatatttta
     4801 ttcattatca acttcatcat tctagcaaca atttaactaa agagaaggga ttggaacagt
     4861 attt
//
LOCUS       DACGLH010000139         4807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.146, whole genome shotgun sequence.
ACCESSION   DACGLH010000139 DACGLH010000000
VERSION     DACGLH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.146"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXT21_002879"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXT21_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ4870225.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IXT21_002880"
     CDS             complement(3250..3768)
                     /locus_tag="IXT21_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ4870226.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          729 g         1713 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACGLH010000140         4634 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.147, whole genome shotgun sequence.
ACCESSION   DACGLH010000140 DACGLH010000000
VERSION     DACGLH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.147"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            175..519
                     /locus_tag="IXT21_002881"
     CDS             175..519
                     /locus_tag="IXT21_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ4870227.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="IXT21_002882"
     CDS             520..939
                     /locus_tag="IXT21_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ4870228.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="IXT21_002883"
     CDS             complement(1031..1381)
                     /locus_tag="IXT21_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870229.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2678)
                     /locus_tag="IXT21_002884"
     CDS             complement(1347..2678)
                     /locus_tag="IXT21_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ4870230.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2934..3500
                     /locus_tag="IXT21_002885"
     CDS             2934..3500
                     /locus_tag="IXT21_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4870231.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3645..3732
                     /locus_tag="IXT21_002886"
     tRNA            3645..3732
                     /locus_tag="IXT21_002886"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3677..3679,aa:Ser,seq:cga)
     gene            4066..>4634
                     /locus_tag="IXT21_002887"
     CDS             4066..>4634
                     /locus_tag="IXT21_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ4870232.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaaacac ctgtaaaacc aagataaaaa tactttcttt tctgtgaaac gattcgtttt
     2641 tgaggatgaa tataaataac gtcagtttcg ttttccatta cacaccttct ttttcatttt
     2701 ctttaactat aacttgaaat gaaagatttg gacaactatc atttaggtca aaactaaaaa
     2761 tgtaagggaa cccattgaat ttaaccaaat ttttttaatt ttgcaaaaaa aagttaaaga
     2821 agactgaaaa aaaatttaaa atataacgtt ttgagtaaaa tgtttgcaaa aaatgctaaa
     2881 aatagggtac aataaaacaa catatactgc agtaaagaag tgggggagtt ttaatgagaa
     2941 gaaaagtcta taccaaggat caaattctaa aagctgctta tgatgttgtt gcaaaggaag
     3001 ggtttaaagg atttactgcg agaaatattg ctaaaaagat ggggatttcg acacagccga
     3061 tttatctgga attcaaaaac atggaagatt taaaaaatac gttgctggat tctatctttg
     3121 aacaactaaa agaagatgtt ttccctattg aacgtactgg tgataaaatc attgatctcg
     3181 gtttgaacta tatctacttt gcaaaaaagc atcatagtct ttacatggca ttgtacattg
     3241 atgaacatgg ggacggaaag aaaatgcatg atttttcata caattatttc cgtgaaatag
     3301 ttaaagaaga tgaaagatac aatcaattat cggatgaata tgtcgatgct ctgcatactg
     3361 gtacatgggt agcagtgact ggcttagcgt cattgatgtc atcaaacatc atgcatccaa
     3421 cagaagaaca aatcattgca ctgatcaaac ggacaattga ctcgatttta gcattagata
     3481 atcctgaaat aatcagatag tgttctatca gtatgaattt gatcggtaag tccgttggct
     3541 gcaaactgtc aatggacttt tttgctaaaa aaagcgattt ctgttattta tctattagaa
     3601 ataggcttga catttcgtga aaaaagttta aaaatagtac ttacggaagg ttggcagagt
     3661 ggtaatgcac cggactcgaa atccggcgaa ccggctaata ccggcgcgca ggttcaaatc
     3721 ctgtaccttc cttatatcaa cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat
     3781 aagaacggcc aaacaaaaat agcttttcct attttttcgt ttgattgggc ttattaccgc
     3841 agatgcaagc ttttgaacac cgtttgttcg gaataagggg ctgtgacaag acttttgtca
     3901 cagcccctta ttgttgtact tttaacctac aatatttttt gattccccct gagtactatt
     3961 ttttataaat gctagtaaat caatcttctt tggccttttt ttgataaaaa aaatactaga
     4021 tgtaagataa aagtgtaaaa acgatttgtt ttaggagggg aaacgatgtg taccgcaatc
     4081 acttatgcaa cgaaagacca ttattttgga aggaattttg attatgagat gtcttataat
     4141 gaagtggtca ctattactcc aagaaattat cgtttcgatt ttagaaaggt aaaaaattta
     4201 gataaacatt atgcaatgat aggaatagca gctggggtat ccaactatcc actctattat
     4261 gaggctacaa atgaaaaagg gttgagtatg gcaggactga attttcctgg caatgctgat
     4321 tataaagaat tacaagaagg aaaagataac gttgcaccat ttgagtttat tccatggata
     4381 ctagggcaat gctcaaccat agacgaagct aaagaattat tggctactat caatctagtc
     4441 aatatcgatt ttagtgaaaa actacccttg tcacctttac actggttatt agctgataaa
     4501 gaaaaatcga tagtaattga aagtatgaaa gatggacttc atttatatga taatcctgtc
     4561 ggtgtgttga caaataatcc gccatttgat tatcaattat ttaatctgaa caattatcgc
     4621 tcgctatcaa atgg
//
LOCUS       DACGLH010000141         4416 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.148, whole genome shotgun sequence.
ACCESSION   DACGLH010000141 DACGLH010000000
VERSION     DACGLH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.148"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            59..913
                     /locus_tag="IXT21_002888"
     CDS             59..913
                     /locus_tag="IXT21_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4870233.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="IXT21_002889"
     CDS             1010..3259
                     /locus_tag="IXT21_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ4870234.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="IXT21_002890"
     CDS             3262..>4416
                     /locus_tag="IXT21_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4870235.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DACGLH010000142         4372 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.149, whole genome shotgun sequence.
ACCESSION   DACGLH010000142 DACGLH010000000
VERSION     DACGLH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.149"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            60..260
                     /locus_tag="IXT21_002891"
     CDS             60..260
                     /locus_tag="IXT21_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870236.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            394..1620
                     /locus_tag="IXT21_002892"
     CDS             394..1620
                     /locus_tag="IXT21_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870237.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2470..3795
                     /locus_tag="IXT21_002893"
     CDS             2470..3795
                     /locus_tag="IXT21_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ4870238.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            3788..4135
                     /locus_tag="IXT21_002894"
     CDS             3788..4135
                     /locus_tag="IXT21_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870239.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT         1710 a          541 c          832 g         1289 t
ORIGIN      
        1 acttgtatac acgtattcaa ataaaaaagc agttattaca tgaaggatta agtggtaata
       61 tgttaaaaag aattgcatgg gataaagata ataaaacaga tattgagctt aaagaatata
      121 aatacaatta tttctcgaga tgtatagata gaaagttact gtttaaaaaa gaaggattag
      181 aatttagttt tgatgaagat gaatttgaaa aattagtgat ggactggcgt aagaatttac
      241 tggatacggt acaattctaa aagcaattga ggtcgaaatt ccgatacttg aatcatgatc
      301 tatattatcg tgttatgagt gaaatttcta ataaagataa gatatttcat ttggacagtc
      361 atgtgaatat gagcttaaca attaaacgta tttatgatct tttatatgtt tataaacatt
      421 aatgaggtgc taaaattgac aacaataact atgacaaaaa aaatagaagt atatgttgat
      481 ggatcacaag aagaaaggga ttcctttttt gatttttttt ggaaaaatag tagagaatat
      541 gtaaaagcgt tgaatctggt atataaacag ctaatgtttg aagagttgat aaaagatgca
      601 attaaagaaa tggatcaagg ttatgctgag agagaagaaa gtattcaaaa tcggattgaa
      661 aagttagcat atactgatcc aatagatcaa aaaaaacttg ataaagcaaa agctgaatta
      721 tctaaattga gaaatgaaaa aaataaagaa gcgagagaaa tattaacgca agcgataggg
      781 ttgaagagtc aaacgagaac acgagatgta gcaaaaaaat tagactttga ctttggagat
      841 tttgttgatt ccgcaaatat gaaagctagt aaagattttg gcaatgattt tatcggaatt
      901 gtgaccggac aaagaacgcc tagaatatat aaaaatagaa acaaatatgg attactacct
      961 tttagaggtc gagatattaa aatccataaa gatgataagt tttatattta tttacctaaa
     1021 ggctttaggt ttaaagtgaa tttaggtagc aagccaagaa aagcaagaga tgttgcttca
     1081 gttttacaac acattatgga tggcgattac aaattaaatc aaagtaatct tatttttgat
     1141 ggaagaaaat tattttttaa tctaacaatt tcttttgaaa aaagctataa tgaaatggaa
     1201 ttcaagaaag gtaatgaatt gatcgtaaac tttgcagatt tgtgtagcgg tgaatgttta
     1261 attaatgata aatctgtaat gtcttttggt gatagccaat atataagaaa cttcaaaagt
     1321 aaaattttag caatgaaatc aaaagaacaa agccgaggag tcttttctaa gggcggtcat
     1381 ggtcgaaaaa gaaaattaga tcaagatcga tgggaagcaa taaagaaaag agaaagtaat
     1441 tttgttaaaa cttataataa tcaatgttct gcaaaaatcg ttaaaactgc tattcgcaat
     1501 aaagctcaaa ttattcgtat cattgaacca gataaggctt atggcgaatg ggcttactat
     1561 caattgaaag aacaactagt aaacaaagga aaaagatatg ggatagaagt cagaagttag
     1621 taaattaaac aatcgttgat ttggcaacga aatagtgagg gaaaatccac tcactgaagg
     1681 gtaaactccg tcaagttgtg tgaccagacg gaaggacgaa tgttgttgag gttttagaac
     1741 catataaaac agcatgtttc tcaaattttt aggataaggg aaattgctag cagtttgttt
     1801 tagaaccata taaaacagca tgtttctcaa atgagtactt taaattaatt gatggcgttc
     1861 ctgctttaga accatataaa acaacatgga tttaaaacaa gaagtaagtc agttctcctt
     1921 ttactttagt aaggaaaaat aaagtattct caataaaact taaaataagc aaacctttgt
     1981 tttaatagct aatcgaagta aaacccaaag ccatatgaac ccctgaatca gcgaaagttt
     2041 atgatgattt ggattactaa tagtttagga attagatggt aaatctaaat taaacaatcg
     2101 ttgatttggc aacgaaatag tgagggaaaa tccactcact gaagggtaaa ctccgtcaag
     2161 ttatgtgacc agacggaagg acgaatgttg ttgaggtttt agaaccatat aaaacagcat
     2221 agttctcaaa ttgtttaaaa tgattgtcaa gttaaaaaaa tctgggatca tataaaatag
     2281 tatagttctc aaatatgatt agaaacccta ttaaagagca atattaagcc aacttactta
     2341 aatcgtttaa ttaagaacaa taatctcttt tagtcagact tttagtctga cttttttgta
     2401 tttccaaaat ttattcttta caaaaacgaa catacgtttg tataattgct tgtgaatggg
     2461 ggaataaaaa tgtttgatta cagtagagaa ccggtgaatg actatttttt aatcgacatg
     2521 aagtctttct atgctagcgt ggaatgtatt gaacgaaatt tagacccaat gactacaccg
     2581 ttaattgtca tgagtagatc ggacaacact ggttcaggtc tgactctagc ttcttcacct
     2641 actgcaaaga aaaaatatca tattactaat gttagtagag ctactgattt accttatcca
     2701 ttaccaaaag aacttcatat tgttccacca cgaatgaact tatatataaa aaaaaatatg
     2761 gaagttaatc agatttttag aaagtatgtc gcagatgaag atttattgat ttattcgatt
     2821 gatgaatcaa ttttaaaagt gacaaaatcc ttaaatttat ttactagttc aggaacaaga
     2881 agcgagagaa gaagagaatt agctaaaata atcaaagatg agattcagaa agaaacaggt
     2941 ctaatagcaa cagtcggaat aggagataac ccattattag ctaaacttgc actggacaat
     3001 gaagcgaaga gtagtaaaga atttatagct gagtggactt atgagactgt acctgaaaaa
     3061 atatggaaaa taaacgagat gactgacttt tggggcattg gatcacgcat gaaaaaaaga
     3121 ttgaatcata tgggaattat gagtatagaa gatttagcaa attggaatcc ctacacaatt
     3181 aaaagtcagt taggtgtaat cggactccag ttatatttcc ataccaatgg aattgatcgt
     3241 acggatattg ctattccacc agaaccaacc aaagaaaagt cttatgggaa ttcacaagtt
     3301 ctaccaaaag attatacgag gagagaagaa gtagaactag tagtcaagga gatggcagag
     3361 caagtagcca ttcgaattag acaacacaat tgcaaaactg gttgtgtacg cttagcaatt
     3421 ggtacgtcaa ttttggaaga aaaatcaggt ttctcgcacc agatgaaaat accaattaca
     3481 gataatacga aagaattaca aaattattgt ctttttttat tcaataagtt ttatgagggg
     3541 caggaagtac gacatatagg agtaacttat agcagattag tatacacaga ttccctacaa
     3601 ttagatttat ttagtgatcc aagaaaaaag attgctgaag aaaatttgga taggattatt
     3661 gataagatta gacaaaaata tggatttaca gctattatac atgcttcatc tatgttagaa
     3721 ggtgcaagaa gtattacgag aagcacttta ataggaggac acgcaggagg attaggaggt
     3781 ttgcataatg attgaatata acgatagagg acgtttaaaa tggtcgggat tttatttgtc
     3841 tgatcatact gaaagaatca atgcagacaa tgtacaacgt gctaatcata atttggcaaa
     3901 agaacaaatg acgactgaag agattgcgaa aatactgaga actgctgtca taaaaaatag
     3961 acccgtctgc attcaaaaag aggaacgaaa cgctgaaggt tactatccag atgatgtagt
     4021 aggaaaaatt attggatatg acgagcttgg tgtatttgtg gaagaagaaa aagttcacta
     4081 tgatgaaatt aggaatgttt ctttctcgga attattaaaa tggagtcaga tatgaacatt
     4141 attaaaaaat acgaattcgg ttatattacg tatgaacagc taatagagga attatgggga
     4201 tatggtcaac aattaattaa tgaagtgggg attgattgct tttgtttcta tattgaatcc
     4261 ggagcaggat accataagaa cagatactat atatttccat atccaccaaa atagtctctt
     4321 agatattttt aaattttctc caaatacttt caattattgt gaaatatttc cc
//
LOCUS       DACGLH010000143         4343 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.150, whole genome shotgun sequence.
ACCESSION   DACGLH010000143 DACGLH010000000
VERSION     DACGLH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.150"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(387..1853)
                     /locus_tag="IXT21_002895"
     CDS             complement(387..1853)
                     /locus_tag="IXT21_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4870240.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IXT21_002896"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IXT21_002896"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ4870241.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1306 a          928 c          698 g         1411 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacatgact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaa
//
LOCUS       DACGLH010000144         4095 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.151, whole genome shotgun sequence.
ACCESSION   DACGLH010000144 DACGLH010000000
VERSION     DACGLH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.151"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            114..1463
                     /locus_tag="IXT21_002897"
     CDS             114..1463
                     /locus_tag="IXT21_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4870242.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1591..2181)
                     /locus_tag="IXT21_002898"
     CDS             complement(1591..2181)
                     /locus_tag="IXT21_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ4870243.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2328..3680
                     /gene="brnQ"
                     /locus_tag="IXT21_002899"
     CDS             2328..3680
                     /gene="brnQ"
                     /locus_tag="IXT21_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ4870244.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1184 a          747 c          792 g         1372 t
ORIGIN      
        1 tgaattactc ataaaaggta aatttctgaa aattttggaa tcttagttgt gtttttcaga
       61 aaactctctt aatatggaag taattaaact tttttagtta ggatgattta aaaatgaatg
      121 aagagcaaga actgcagcgt ggactgaaaa atagacatgt ccaactgatt tctattggcg
      181 gagcgattgg aacggggttg tttttaggtt ccggaaagtc tatccagcta gctggccctt
      241 cgatattgct cgcctatctt attactggat gcatctgttt tttcattatg cgtgcgttag
      301 gcgaacttct gttatcaaac accaataacc attcttttct ggattttgtt gcagaatatc
      361 ttggcaaaaa agcggctttc atcactggct ggacgtattg gttctgctgg gtatcgattg
      421 caatggccga tttaacagcc atcggaatgt atgtacgcta ctgggcgcct ggtgttcctc
      481 agtggcttcc tgaactgatt gcattaggtc ttcttctttg cttgaatatg gtggctgtct
      541 cattgtttgg tgagttagag ttttggtttg cattgatcaa agttgtagcg atcattgcct
      601 ttatcctcgt aggagcttat atgatcctca cccactatac gacagatatt ggcacagcca
      661 gtatcacgaa tctatggtct catggcggat tctttccaac tggtgcaaaa ggatttatct
      721 tagctttcca aatggtaacc tttgcttttg ctgggataga attagttgga ttagtcgctg
      781 gagaaacaga aaatcctgaa aaagtgttgc cggaagctat caataatatt cctatcagga
      841 tcatcttatt ttacttagga tctctttttg tcattatggc aatctatcca tggaacagct
      901 tgaatccaga taacagtcca tttgtagaag tattttcgga aattgggatc acgattgctg
      961 cttccttgat caatttagtc gtattaagtg cagcagcttc tgcctgcaac agtgcaatat
     1021 atagtacagg acgtatgctt cgttctttag cgcaagaagg cagtgcccct aaaaaattca
     1081 aaaagttaac aacacatcat gtgccaggga atgctttgac attttctact atcgtcattt
     1141 tcatttctgt tatcttgaat tacgtgatgc ctagtgaagt attcacacta gtaagcagta
     1201 ttgccactac ttgtttcttg tttatctgga gtatgcttgt ctacacgcac atgaaatatc
     1261 gtaaaagtat cctcggcaaa aaagctcatt catttaaaat gccgttgtac ccatttagta
     1321 attatctggt atttctttac atggcctttg tgtgtgtcgt cttgttccta ggaaaagata
     1381 cgagaatcgc tttattacta acacctgtgt ggttcttgct tctattacta atctatcata
     1441 tgaaatatga acgtaagaag taagaaagta gttgtgacag aagtggtcag cttcgagaaa
     1501 taagaaggaa tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttcttg
     1561 gagataaaag cttctgtccc agcctctttt ctatttcccc ggaaacaacc gctgataata
     1621 ggcatctgct tttttatagg aaaaaatcca aataccagat acaaaaaaac gtccgaataa
     1681 cccgatcaac ggtacaaaaa agacaccggc aatcaagcat aagttaatca tcatggagat
     1741 aagacttcga cggtcctttg acaaaactct cacgacatgg gaatctggtt tatttgcttt
     1801 catgatttgc ctcatcaaga taccaaaaga aaaactccac atcgtgtata ctaagacata
     1861 agccatctca ggccaaaaag cttcagggtt acgcccgacc caactagtaa cagaaggaca
     1921 taaagtcagc caaaagagca aaaagatatt cgaccagacc actcggtaat taattcgttc
     1981 tgccatctga aatatacgat gatggttcac ccagatcacc gcaacaaata taaagctgat
     2041 ggtataagct aataatgtat ttcggatgac ccataaagaa tgaaagctag atgtattagg
     2101 gatcttgatc tctaatacaa taactctcaa aacaatggct accactccat cagtaaatgc
     2161 ttctaatctc gactttgtca cgatgattcc tcctcagatg attcagcgct tttattttat
     2221 cacttacgtt attaaataac catcgtttta tatagggaat tttctgaaaa ttgttgcata
     2281 tttttcgaaa ttttgttact tttaaaaaaa ccgtaaagga gttatgtatg acaaaaaaat
     2341 tatcatttaa agactatctt tttataggct ccatgctttt cggtctattt tttggtgctg
     2401 gaaatcttat ctttcccgtt catttgggtc aagaagcagg cgctgctacc ttttgggcga
     2461 atctaggttt cttggttacc ggtatcggtc ttcccttctt aggggtgatt gccattggtg
     2521 tatcgaaaag ctcaggtgta tatgaattag caaaacggat aaatcaaaca tatgcgatgg
     2581 tttttaccat cttgctttac ttagttatcg gaccgttttt tgctttacct cgccttgcaa
     2641 ccacatcttt cgagattggt ttagcaccat ttatcgatcc tagcagacag acgctatatc
     2701 tagcaatttt cagtattgta ttttttgtgc tggcttggtg gttttcaaga aaaccgacaa
     2761 agatattaga ttatgttgga aagttcctga atcctgcatt tttggtttta ttaggtgctt
     2821 tgcttctcct tgcttttttg aatccactag gttctgtaaa tcaagcgcct attcagccaa
     2881 attatcaaga gcatgctttt ttcacagggt ttactcaagg atataacaca ttagatgcgc
     2941 ttgctgcttt agcatttggg atcatcattg tcacaacaat ccaaaacatg ggtgtaacaa
     3001 aacctgctga aatcgctaaa gacacgatca aatctggtgc gatcagcatt atattgatgg
     3061 gaatcattta tactttgctc gcttatttag gtgctatgag cttaggtagc tttgctctaa
     3121 gtgaaaatgg cgggatcacg ctggctcaga ttgctgatca ttatttaggt acttatggca
     3181 gtatcttatt agcatttatc gttatattag cttgcttgaa gaccggtatt ggtttgatta
     3241 cagcttttgc agaaacattc acagatttgt ttccgcaaag aaattacgca ttctttgttg
     3301 cacttgctag tatccttcct tgtttagcgg caaacgtagg cttgacgaat atcattcaat
     3361 tttcgttgcc tgttttgatg ttcatctatc cattagcaat gacactgatt ttactagtgt
     3421 tagttggtcc tttgtttaag cagcgtacgg ctgtttatcg aatgactact tattttacat
     3481 tgattgcttc gatttttgat gggttgaacg cttgcccaga aactatcaaa caaacaccta
     3541 ttgttcaaaa tcttttatat gttgcggaaa gctacttgcc gtttttcaaa ttggggatgg
     3601 ggtggatcgt accagcagtt atcggttttg ttatcggact gatttggagt ttcgctaaga
     3661 aagaagaagt agcagattga taaaagaggt tgtgaaagag acgttcagcc ttgagcaata
     3721 aaaagtaatt ttgaaaaatg gcttctcaca tttttgcata aatttgactt aataccgaga
     3781 aactgacttt tgaacaccgt ttattgagag gtcgtgaaaa agccgcttag tttcaagtat
     3841 taagaaatat tttgtgaaaa tggcttctca catttttgca taaatttgac ttaataccga
     3901 gaaactggct tttgaacacc gtttattgag cgtctataaa ctgacctgag tattagagaa
     3961 acaaaacggc aaagtagctc catatacttt gccgttttgt tttgaactaa taccgaagag
     4021 aagctgatca aacaacattt cttttataat atagatttta gacatatata tgatataaaa
     4081 atagttttcg caaaa
//
LOCUS       DACGLH010000145         3718 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.154, whole genome shotgun sequence.
ACCESSION   DACGLH010000145 DACGLH010000000
VERSION     DACGLH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.154"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..353)
                     /locus_tag="IXT21_002900"
     CDS             complement(<1..353)
                     /locus_tag="IXT21_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870245.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="IXT21_002901"
     CDS             complement(613..1161)
                     /locus_tag="IXT21_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870246.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="IXT21_002902"
     CDS             complement(1162..2019)
                     /locus_tag="IXT21_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4870247.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="IXT21_002903"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="IXT21_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="IXT21_002904"
     CDS             complement(2581..2910)
                     /locus_tag="IXT21_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4870248.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="IXT21_002905"
     CDS             complement(2987..3259)
                     /locus_tag="IXT21_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ4870249.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="IXT21_002906"
     CDS             complement(3259..3522)
                     /locus_tag="IXT21_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4870250.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DACGLH010000146         3633 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.155, whole genome shotgun sequence.
ACCESSION   DACGLH010000146 DACGLH010000000
VERSION     DACGLH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.155"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(45..1223)
                     /locus_tag="IXT21_002907"
     CDS             complement(45..1223)
                     /locus_tag="IXT21_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4870251.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSFYSKSSEIKVFIKEKNNKNEIQ
                     IKDNINVNLESDSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLL
                     ISILFSAIISLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDL
                     YSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYK
                     ERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQ
                     KKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYG
                     NNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS
                     "
     gene            1253..2431
                     /locus_tag="IXT21_002908"
     CDS             1253..2431
                     /locus_tag="IXT21_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAQ4870252.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            complement(2491..2697)
                     /locus_tag="IXT21_002909"
                     /pseudo
     CDS             complement(2491..2697)
                     /locus_tag="IXT21_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(2694..3347)
                     /locus_tag="IXT21_002910"
     CDS             complement(2694..3347)
                     /locus_tag="IXT21_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4870253.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         1214 a          621 c          451 g         1347 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaacga gagtgtaaaa tattttgtgt aaatagaaaa aaggaagtcc
     1201 cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc atatgaacga
     1261 ttttactaca gaaattctaa agactctagc gaacaaaggc gatttgaatg aattattccg
     1321 tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg ctttcctcga
     1381 ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg gctcctatga
     1441 ccgtacggtc aagaccgagt acggggaact tcatctccag attccgcgcg accgcaacgg
     1501 cgagttcaag caacagactg ttcctgctta tagacggacg aatgatacct tagaggagac
     1561 cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact tgattgagaa
     1621 aatgtatggg catcactaca cgccccaaac catgtccaac atgactaaag tgctgactga
     1681 agaagtaaat gcatttaaag ttagaactct aaatgataag tatgtcgcta ttttcatgga
     1741 tgctacttat attcctttaa aacggcaaac cgtctccaaa gaagcaatct atattgccat
     1801 tggtatacga gaagacggca ctaaagaagt actaagttat gcgattgccc cgactgaatc
     1861 gacctacgtt tggaatgagt tgttacagga catttactcc agaggggttc atgatgtctt
     1921 actgtttatt accgatggct taaaaggcat gaaagatacg attcaccaga tttatcctaa
     1981 agctaaatac caacattgct gtgttcacgt ttcccgtaat attgctcata aagtacgtgt
     2041 caaagatcga aaagaaatct gtgatgattt taaggctgtt tatcaagcta gctcaaagga
     2101 agaggcaaat acctttttag ggagtatgat tgagaagtgg cagaaaactt atcctaaagt
     2161 gacgcagtca ctgataaaaa atcaagattt attgactttt tatgagtttc cgcctggtat
     2221 ccgccgaagc atttactcaa ctaatttaat cgaatctttc aataaacaaa tcaagaaata
     2281 cagccgcaga aaagagcagt ttcaaaatga agaatcacta gagcgtttcc tagtatccat
     2341 ctttgataca tacaatcaaa aattcttaaa tagaagccat aaaggcttcc aacaagtgac
     2401 ggatacatta gcttcaatgt ttactgagta acccattatt ttgcagaagg acgagttatt
     2461 tacacaaaat tattgacgct cccaataaaa ctccaaagtt tgttctatat attttatttc
     2521 ttttccattc atattactag aaatttcatt tgccatatta tgaatctttt cttttctcgt
     2581 ctccaaatat gttttaggaa agataaaaaa aattaatgaa tgaactaata taattattat
     2641 tccaagaaca gaaaatattt gtatgaacat ttttggaaat atctttaatt ttttcatttt
     2701 ctctccaatt tatatcctac atttcttata gtagttatac agtctaattg taattttttt
     2761 ctaagttctt ttatatatac atctattact cgatcataag gagtttcttc gctatctttc
     2821 catacataat caataatttg cattcttgtt aacacttgtc cattattatc caataaacat
     2881 tttaatactt ctagttcttt tgcatttaca tctatttctt caccatttat tttagctgta
     2941 tagctattaa aattaaccga tagatcctta tattcaaact tctctaaatg tccataattc
     3001 ttcttaatta aagaatctat tctagctttt aagaccggca atgaaaatgg cttttctaca
     3061 tatccgtcta ctaaattagt aaatgcatca attttatatt cttcatcact aaatgcagtc
     3121 aagattagaa ttggtaaatt gcttttcttt ctaatttctt tcaacacttc taaaccattt
     3181 ataaaaggta tctgaatgtc taagataacc aaatttatat cgctattaaa ttttgacaag
     3241 gcttctcttc catctttagc ttgaataaca gtatatccaa attctgaaag atattcactt
     3301 attccctctc ttatcatatt atcatcttca acagttaata ttttcatata tacctccact
     3361 taagcctata cactagatat tatacagcat attatggctc tataatatct agtgttggtg
     3421 acaccttctg ttgccaatac tttttctttt ttctaataaa tgcaaacaga caccttgaaa
     3481 tcctttagaa taaagggttc tataatattt tgtgttgttg gaaaatcgaa ggcgattttc
     3541 tactaacact ttttcttcgt ttcaactcaa aaagagactc acttttggta aaatttagtc
     3601 gaccaaaaca tcaccctacc aaaggagtcg ctc
//
LOCUS       DACGLH010000147         3523 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.157, whole genome shotgun sequence.
ACCESSION   DACGLH010000147 DACGLH010000000
VERSION     DACGLH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.157"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            11..610
                     /locus_tag="IXT21_002911"
     CDS             11..610
                     /locus_tag="IXT21_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4870254.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            691..1704
                     /locus_tag="IXT21_002912"
     CDS             691..1704
                     /locus_tag="IXT21_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4870255.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            1812..2729
                     /gene="rbsK"
                     /locus_tag="IXT21_002913"
     CDS             1812..2729
                     /gene="rbsK"
                     /locus_tag="IXT21_002913"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ4870256.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            2757..3152
                     /gene="rbsD"
                     /locus_tag="IXT21_002914"
     CDS             2757..3152
                     /gene="rbsD"
                     /locus_tag="IXT21_002914"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ4870257.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            3152..>3523
                     /locus_tag="IXT21_002915"
     CDS             3152..>3523
                     /locus_tag="IXT21_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HAQ4870258.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSG"
BASE COUNT         1185 a          688 c          703 g          947 t
ORIGIN      
        1 aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa aaactgcttt
       61 tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc cacacactgc
      121 agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta ccgctgcctt
      181 gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac aaactgatat
      241 gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga aggattcttt
      301 gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt tttttggtac
      361 ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa gcggtgtagc
      421 gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt accatatcgt
      481 gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt tcataaaaaa
      541 gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag ctatccaaca
      601 aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt catgggaaaa
      661 taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac aatcaaagaa
      721 gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa tggaaatacg
      781 gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga attaggctat
      841 cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat tggtgtactc
      901 gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga ggatatcttg
      961 tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca aaaagaaatc
     1021 gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc gacttctgct
     1081 gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt cattgttttg
     1141 gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt tcgcggtggt
     1201 tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct tgtctatcct
     1261 gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc tcttgatgtt
     1321 taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa acaaggtggc
     1381 taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc tttaaatgat
     1441 gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat ccctgaagac
     1501 tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc gaaactgaca
     1561 acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt gttagatcgg
     1621 atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt agaaaaaaga
     1681 ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc gaactattaa
     1741 aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg agatagttgg
     1801 aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac actacccttc
     1861 gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt tttcacgctg
     1921 gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca cggacatctt
     1981 ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta ctgacaaaag
     2041 aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga caagcaatga
     2101 ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca aataatgctt
     2161 tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac tttattattg
     2221 ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca aaagacgcag
     2281 gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact gaactacttg
     2341 aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca ggaatcagag
     2401 tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta ggtattggaa
     2461 cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa gaacacggta
     2521 tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat acatttattg
     2581 gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg atcctttatg
     2641 gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct atcccttacc
     2701 gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa acaaacatga
     2761 aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg gggcactttg
     2821 ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa aaaatagatt
     2881 tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt ttagaagaac
     2941 ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg gaaatattgg
     3001 aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat gaagaaatga
     3061 aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca ccttacgcca
     3121 atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc ctgaacagat
     3181 ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag acaatattga
     3241 ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc cagtagccaa
     3301 tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac tcgatagata
     3361 tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga cgcgtcaaat
     3421 cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg gtttgatatc
     3481 ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggt
//
LOCUS       DACGLH010000148         3292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.158, whole genome shotgun sequence.
ACCESSION   DACGLH010000148 DACGLH010000000
VERSION     DACGLH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.158"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            109..321
                     /locus_tag="IXT21_002916"
     CDS             109..321
                     /locus_tag="IXT21_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870259.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(599..2398)
                     /locus_tag="IXT21_002917"
     CDS             complement(599..2398)
                     /locus_tag="IXT21_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ4870260.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2522..3118)
                     /locus_tag="IXT21_002918"
     CDS             complement(2522..3118)
                     /locus_tag="IXT21_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ4870261.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1192 a          594 c          580 g          926 t
ORIGIN      
        1 tcaaaataaa aaataaaaaa atggataaaa acgttttcaa atacaaggta atttgttatt
       61 ataaaataaa gaaaaaacaa aataaaaata aagaaaagag ggaatgaaat ggtaaaagca
      121 gaagtaggaa gatcttatat atgcaaaccg atcggagtat ctcatgaact aatcggctgt
      181 attgaacgta catatatcaa cacggctgtc gtgtatgttg aaagttatga ccaacgagac
      241 tatcaactta tcaatgaaag caagtatcgc atgctcgtaa aattctgtga tattagcgca
      301 cctgctgaat tggtttctta aacagtattt ttgagaattc gcaagaactc ccttataata
      361 taaataaaga cagaagggag cgagaagata tgccaaaaag aaaaagttca gggaaaatgg
      421 gaattattct tgcagtgatc tctgcaattc ctgctatttt agctattgtt cgtgcgatga
      481 aggaatcaag agaaagttaa gcacacatta tgagcgtcaa acaaaaaacg catatacctg
      541 cggtggcagg ttatgcgttt tttgttgtat aaagactttt ttcattaacg atttttgatt
      601 agccgactcc caaaacaaaa tttagcagtt tatctgaata agtgatttct tcatctgttt
      661 gtttcacact ttcatttagt agtcccatct catcggttat gataccatcc acaccataaa
      721 acatcatgcg actcatagca tctgaatcgt tgattgtcca tacatatact tcttttccgt
      781 cttggtgggc agaattaatg aagttacggt tgatcgtact atattccatt gtcagaaaat
      841 cagcaggcgt cacagggggc ccaacaatat tgaaaggtaa gatgtagccc acataaaagt
      901 ttggttctgc ttcttttaaa tctgaaacgg tttggtacgt caaactttgc aaaatatgat
      961 ggttctctaa gataactttg cggtatttat ggacaaatcg ttcgacaagg tcatccgaat
     1021 cttttttaga ggtcttgatc tcaataagta atttttggtt tagcttatgt gctttttcca
     1081 aataagaatc aaaagaacaa acaggggctt gcactccgtt ttcagtaact gtaagatttg
     1141 tcaattcttt caatgtaaga tcctgtggtg ttccatttac ccctgttaag tgacgaagat
     1201 ttgcatcatg catcactacg aaatgaccat cttttgtttc ctgaacatcc atctctacat
     1261 aatcaggatg atagtctctg cttgtttttt caagtgcttc taaagtattt tgcgccccat
     1321 tttgggtact cacacctctg tgagagacag tcactggttt tgtctgaaca gctgaagtca
     1381 gataatccat attgtaaaga cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac
     1441 cagtgttttt cagcgcagag aaacgcaagt tgggggcttg tgggataaac cacttaggga
     1501 tctcaggcaa gaagccttcg tcatccatga aatcaacaat gatataaaaa atccctacgg
     1561 tagacatcac gatattgaat aataaaatcc cttgtaaaag cgtcatagca aacacagcgc
     1621 tgatcaatgc atagtctggg aaaaattgtt caaccatact ttgtgccaaa ataacaacga
     1681 tatatcctaa agaagaaagg agtaaaatcg taccgccaat cacaataaat tggcccgtga
     1741 tggccaaaag acgcgatttg gtcaatgacc agctttcccg aatggctgct cgaaaaggac
     1801 gatctcgtag aatcatttcc ggtaaagcaa aaatcagtcg aatcccaata tagccaagga
     1861 aaatataaac gagtaaaaag gaactgacga tgatccaacg attagcaaaa ataaaatcca
     1921 taatgaaagc ggggattttt atttttgaca gtagatcaga attaaaactt aatccgctga
     1981 tcggtagaat caataaaaaa taagcaagga aaaacaaaaa agtaatgggt ctcactttct
     2041 tcagctgaag tatcgtcaag tgtaagagtt gcttcaaaga gattggttct ttctttttaa
     2101 taaaaaaaac actcatcaaa agaaaagtaa attcgaaata aactaacagt aaaatcgtca
     2161 tcaaaactac gattaacgag agtagaaccc caggatgttg agaaaaaatt gaaggaatat
     2221 tgtcataaga caaatagtca attgcccctc ggtgtaaaat aaaacgggtc gtactgctca
     2281 ataagggtaa acaaataaat aaaataaagc catgcatcag caagacatca cggaaataag
     2341 cttgtgttcc ttttaaaaag tcccaagcat tacctaagct acgtttcaaa taattcatcc
     2401 tatcacctgc tcttcacatt gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt
     2461 tttgcagacc ctttcccaga tttaacattg tcaatcaatg actaatttta caaaataaat
     2521 attatccaag aagaccactt agatattgac ctccataaat caaggccata ctatataaga
     2581 aaatcgacag tggtttagca atcaaaatga ttagtgtata tcgtttcact gacatgtttg
     2641 tcaatccagc catcaaacat aacgcatcgt caggagcaac tggaagaaaa atagctagag
     2701 caaacagctt ctcaaaacgg ttttgattat ccagccagcc aatatatttg ttatatgtct
     2761 tatcgctaat catggaaagt atgaatggct ttccataacg acgccctagc agaaaaatga
     2821 taatcgaacc aatgcagatt ccaatatagt tatagataaa tccagcataa gggccaaata
     2881 tcaggacacc agctgcagta ctgatccctc caggaatgat tggaataact acttgcagga
     2941 tttggatgaa gacaaaaata atcggtccta aaatgatgga atcacctact aatcctctta
     3001 acgcattcaa atctttgaaa acacctaaat taataaaata aatcgtcagc gcgattgata
     3061 ggcctaatcc gataatggat atgaaattta tcagttttcg agaggcagca atattcatta
     3121 ttcatcaact ccttagaggt tctttgttaa ctatacttta ctagaaaagc aactaactgc
     3181 gccatttttt gatatgacag aaaacaaaca attttgtaag ttcatcataa gtataaaaga
     3241 ttgatctcaa aaaaacatcc aactaaagac ttatttaata acttgttttt tt
//
LOCUS       DACGLH010000149         3242 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.159, whole genome shotgun sequence.
ACCESSION   DACGLH010000149 DACGLH010000000
VERSION     DACGLH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.159"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(552..2165)
                     /locus_tag="IXT21_002919"
     CDS             complement(552..2165)
                     /locus_tag="IXT21_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ4870262.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="IXT21_002920"
     CDS             complement(2306..3214)
                     /locus_tag="IXT21_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4870263.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1045 a          557 c          572 g         1068 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ct
//
LOCUS       DACGLH010000150         3199 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.160, whole genome shotgun sequence.
ACCESSION   DACGLH010000150 DACGLH010000000
VERSION     DACGLH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.160"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            301..585
                     /locus_tag="IXT21_002921"
     CDS             301..585
                     /locus_tag="IXT21_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870264.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="IXT21_002922"
     CDS             588..1004
                     /locus_tag="IXT21_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870265.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="IXT21_002923"
     CDS             complement(1141..1860)
                     /locus_tag="IXT21_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ4870266.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="IXT21_002924"
     CDS             2513..2608
                     /locus_tag="IXT21_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ4870267.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="IXT21_002925"
     CDS             complement(2910..3155)
                     /locus_tag="IXT21_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870268.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DACGLH010000151         3177 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.161, whole genome shotgun sequence.
ACCESSION   DACGLH010000151 DACGLH010000000
VERSION     DACGLH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.161"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..234
                     /locus_tag="IXT21_002926"
     CDS             <1..234
                     /locus_tag="IXT21_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870269.1"
                     /translation="SHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLI
                     ERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            851..>3177
                     /gene="esp"
                     /locus_tag="IXT21_002927"
     CDS             851..>3177
                     /gene="esp"
                     /locus_tag="IXT21_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ4870270.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1164 a          468 c          612 g          933 t
ORIGIN      
        1 agtcatatta atggtagcaa ttttaaacaa acattattta atctgattaa gctagatgct
       61 aaaatatcaa gttatttatt ctttttgata catgatgaat ttatggatta tcaaaaattg
      121 gatcagttaa ttgaaagaga aagctgggaa gattattacg tgggtctaac tttttcagaa
      181 aagttaaaca actacaagtt aatagattat aagtatttga gtgaaagcaa ttaattattc
      241 atgtagagta ctagacactt taaatatgga caattcaatt ataaagaaaa agaatcaata
      301 atttaatgag gctgtgacaa atgtcatttt ttgtttcgct aaaaacgagt gaaaaaatgt
      361 tgataactcc aatcattcgt ttaagattct aggaatcatt cagaaatgct gtgaaaccga
      421 tactttataa ttataaagaa ggtgatttca aattccgaag aaggaatttg aagtcacctt
      481 tttacttatg tcacagtctc attcagtaat gcgggataag gctccttatt ccgttatttg
      541 aatactctga agtttaatct aagaaagggt aatattttga aaacatgctt catcgcatag
      601 aaatgaactt aatttcagaa agaatacata catatctttt tatatgtttt cagctgctga
      661 atggttaaaa agcacaaata tccgaaaaaa acatgaagaa atgtactttt tttgttgtaa
      721 tgttcatgaa attctctaag taaattagtt aacataaggt tagtaagatt cctattctgt
      781 gaataatacg tatcagattt atcttttatt cttactagga aagtaattat taagggagtt
      841 gatttgaata atggttagca agaataataa gagagtattt ctagaaaaaa caaagaaacg
      901 agtattgaag tacagtatca aaaaattaag tgttggggtt gcatctgttt tgattggtgt
      961 tggtcttgtc ttgggaacaa ctgaactagt taaagcacaa gatgaaattt ctccaagcag
     1021 cccacttgaa acagcgataa gctccgtaca agtaggtgac aaagtagctt cggggaatac
     1081 ttttcaggag aatcctggtt atacgaaaaa ttataacttt tcggatttac aatttagtcc
     1141 tcaagaacta actggagaca cactaaaagg caatacaatt ggatttgaag tttacggaaa
     1201 acataatatt gcagcatcaa ctaaaaactg ggaaatccgt cttcaattag atgaacgatt
     1261 agctaaatat gtagaaaaaa ttcaggtcga tccaaagaaa ggaattggat cttctagacg
     1321 gacctttgta agaattaatg attcgctggg tagacctaca aatatttgga aggtcaatta
     1381 tattcgtgca agtgatggat tatttgctgg ggcagaaaca accgatacgc aaaccgctcc
     1441 taatggggta attacgtttg aaaagagcct agatgaaatt tttaaagaga taggcataga
     1501 caatctcaag accgatcgtt taatgtatcg aatctacttg gtaagtcatc aagacgatga
     1561 taaaattgta ccgggtattg atagtacggg ctacttttta acagattcag atgatttcta
     1621 taatagctta gatgtatctg aaaataatcc tgaccagttt aaacatggat cagtaagtgc
     1681 aaaatatgag aaacctaata ctcaaacaaa agatggttca ggatccacag gtgctaatgg
     1741 agctataatc ttagatcata agttaacgaa aaattataat ttctcatatt catcttccgc
     1801 aaagggcacg ccttggtatg ctaattacaa aattgatgaa cgattagttc catatgtagc
     1861 gggtatacag atgcacatgg ttcaggctga caaagtaaca tatgatgttt cgtttgaatc
     1921 cggaacaaaa gtagctgatt tagcaatcga gcgacgggaa ggtcatgaaa attatggcat
     1981 aggttcaata actaataatg atttaactaa acttatcgat tttgctaatg caagtccacg
     2041 tccagtcgtt attcgatatg ttttacaatt gactaagcca ttagatgaaa tcttagaaga
     2101 tatgaaagca acagcacaag ttgaagaaaa taaaccattt ggtgaagatt tcatctttga
     2161 ttcttggttg tcggatacga ataaaaaatt aatccagaac acttatggaa caggttatta
     2221 ttatttgcaa gatattgatg gtgatggaaa ccctgacgat aaagaagaga gcggagacac
     2281 gaatccatat atcgggaaac ctgaattaga agaagtatat gatgttgaca caacagttaa
     2341 ggggaaagta ttcatccacg agttagcggg aacaggtcac aaagcccaac ttgttgataa
     2401 agaaggtact gtattagcag aaaaaactat cgctccaaat gaaaaagatg gggctccaat
     2461 ttcagatact gtagaatttg aatttacggg tgtagattca agtaaattaa tcgcgaaaga
     2521 tgaattaaaa atccaaatcg tttctccagg ttttgataaa ccagaagaag gttcaaccgt
     2581 tattaaggaa tcaccaaaag cggttgataa acaaaccgtg gtagttggat ttaaaccaga
     2641 tgctaaagaa tcaattcgga ataataaaaa cttacctgaa gatgcagagt attcatggaa
     2701 aacagagcct gatacttcta acgttactga tagtacgaaa ggtattgtaa ctgttaagat
     2761 cggaaatcga actttcgacg tggatgtaga gtttgctgta aaagcttctc aagctatgga
     2821 aaatgatgca acatacgtac ctataacaac aactccagaa acgacagttc aaagtggaaa
     2881 acctacattt gataaaccag atgttccttt agctaaagat gccttttcaa tttcagatgt
     2941 ctataataag gacttcggta atgcaagtgt tgatgcaaac actggtattg ttacattcac
     3001 tccagctaaa ggtgtaggag aatcggagcc gattactgga acaattccta ttaaaattgt
     3061 ttaccaagat ggttctgtag gcacgaccga tttagcagta actgtaagta aagatattta
     3121 tgaaaatcca ggagaaaaca ttcctgcagg ctaccacaaa gtaaccttca ccgcagg
//
LOCUS       DACGLH010000152         3125 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.162, whole genome shotgun sequence.
ACCESSION   DACGLH010000152 DACGLH010000000
VERSION     DACGLH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.162"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            288..554
                     /locus_tag="IXT21_002928"
     CDS             288..554
                     /locus_tag="IXT21_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870271.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            612..1124
                     /locus_tag="IXT21_002929"
     CDS             612..1124
                     /locus_tag="IXT21_002929"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4870272.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="IXT21_002930"
     CDS             1121..2158
                     /locus_tag="IXT21_002930"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ4870273.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2318..2494
                     /locus_tag="IXT21_002931"
                     /pseudo
     CDS             2318..2494
                     /locus_tag="IXT21_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            2549..3125
                     /locus_tag="IXT21_002932"
                     /pseudo
     CDS             2549..3125
                     /locus_tag="IXT21_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1159 a          517 c          613 g          836 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacaccgac catcgtccag taaagagacg
     2341 caataaattc tatcgaagtt tacgcactgc ctctacaacg attaaaggca tggaagccat
     2401 ccgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
     2461 tgaaatcaag gtattattgg gaatcccagc ttaaatcata gataccgtaa gggattttat
     2521 tctttattta aaactttgca acagaaccga atcattaaaa aaacattata cagagccttg
     2581 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
     2641 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
     2701 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
     2761 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
     2821 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
     2881 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaaggag attgttattt
     2941 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     3001 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     3061 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     3121 aaaag
//
LOCUS       DACGLH010000153         3012 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.163, whole genome shotgun sequence.
ACCESSION   DACGLH010000153 DACGLH010000000
VERSION     DACGLH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.163"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            178..360
                     /locus_tag="IXT21_002933"
     CDS             178..360
                     /locus_tag="IXT21_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ4870274.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            463..747
                     /locus_tag="IXT21_002934"
     CDS             463..747
                     /locus_tag="IXT21_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870275.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            763..999
                     /locus_tag="IXT21_002935"
     CDS             763..999
                     /locus_tag="IXT21_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870276.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1021..1275
                     /locus_tag="IXT21_002936"
     CDS             1021..1275
                     /locus_tag="IXT21_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870277.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1357..1542
                     /locus_tag="IXT21_002937"
     CDS             1357..1542
                     /locus_tag="IXT21_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ4870278.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1609..2004
                     /locus_tag="IXT21_002938"
     CDS             1609..2004
                     /locus_tag="IXT21_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ4870279.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1994..2371
                     /locus_tag="IXT21_002939"
     CDS             1994..2371
                     /locus_tag="IXT21_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870280.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2372..2767
                     /locus_tag="IXT21_002940"
     CDS             2372..2767
                     /locus_tag="IXT21_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870281.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1059 a          507 c          524 g          922 t
ORIGIN      
        1 gtttatgttt atgtatatgt atatgaatat aaacgtatta ggtacgaaca cttttaaatt
       61 cttttctggg ctttgttttt agggtagggc taaaaaaaac atcaccatct gatccaaaac
      121 agctcagaaa cgcatttaaa cgtatctgat accaatatta cagaaggagt gtatcgtatg
      181 attaacctat ttcttttagt gacagctagt acattattct ttactggaat atcactgata
      241 atttcctatt tgatctataa gaaagccgaa aatcacacaa gcaaagcaat cggactagcc
      301 gtttcatcct ttttaacttt gctgacttta tttgttactt tatcgttttc ttttgtttag
      361 aacagctgat ccattagcac ctttttctct ttacattata cacactgttg tgtataatgt
      421 atttgtaaac aagctaatta acttttagga aggagtgaca agatgctaac agctacttta
      481 cagcaaccta ctcacgttgc tattgcagaa agttatacaa ttaatgcagg gatcgttaaa
      541 cagctgatca aggatcgaga attcaaaaag tttaacagtc ggcaagcgta taacagacag
      601 ctaaagaaag ctattctaaa gcacaaagat tccttcatcg ggaacttgga aagtgtagat
      661 gttaaaactg tttcaacggc attttcagtt gttgaagtga ctattgacgg tcgcttgatt
      721 tctctatttg cttttggaat taattgatag ggagtgtgaa ctttggaagg taagactttt
      781 gcagaaattt ggactgaatt tattgaggta ccaactcgca ttgatccgat aacttctgaa
      841 aaggtattgg atgcagactt ctacaatctc aaagcaggta ctagccttga atgcgtttgg
      901 atgtatttag aagagcattt caaaggcgta acgctcggtc acgtcttata ccacaatggt
      961 gccgagtggg atgcaattgg aaatatccat ttttcttaat ataagaaagg agaacacaat
     1021 atgaattata ccgatgaaga acaaatcgct ttggaatacg ttttcacaaa tgaagaaggg
     1081 gaggaaattg aaaaagtctt gatcatttca tcaaaagact tggagaaggc tttggcttat
     1141 gaaccatacg aatcaaccgt aactgtcatt gaattcttag atacctatac ttctaatgat
     1201 acttgggaaa ttatgaatat ttgcaacgct aataacattc aatttgctga aaaaaccaat
     1261 aaatcatttt attagttttg caataaaaca tcctcttttt ctattgattc catacacaac
     1321 aatgtgtata atagattttg taaagggagg gtagctatgc ctaagacagc aagagaaatt
     1381 gtaaaaattc tcaaatctaa cggcttcaaa aagactggac aaactggcag tcatgcaaaa
     1441 ttttataatt ccaagactaa aagaacggtc gttgttccga tccacaatgg tgatgtacca
     1501 ctaggaacag aagctaatat ctggaagcaa gcagggcttt aacagccttg ccttcctatt
     1561 tggtattagg catactatcc tctcttacac tagaaaagga gcgaggaaat gaagacaaaa
     1621 aatatttttt atccggcaat cttcgagaaa gaaggagaag cttataacat cgttttccct
     1681 gacttaccgg aaatatctac ctttggaaat agtcttgaag aagcatattt aaacgctaag
     1741 gatgctttag gattagcgct atatagtgta ccagatgcag aatttcctaa accatcagca
     1801 gtggaagaat taactttgaa agccaaccaa gtggtagtaa tcatccaact aaacattaaa
     1861 cttttccgaa gaagcttaaa tacaaaaaca attagaaaaa atgttagtgt tccggaatgg
     1921 ttagttcttt tagggaagga gaaaaacata aacttctctc aactacttca aaaagcacta
     1981 gaagaagagt tgcttgaaaa gtaaaaagga gaccgaacct atgaaagcta cacacttaaa
     2041 ctatttctct cgaacgttaa tcaataatct attaaaagat acaactggca ttagcgttag
     2101 taccaaacac tttgccaatg atctaattat ttttgaggat aataacttgc gaattgagat
     2161 gtttattgaa caggcaaccc ctaactcaat acctgaattc tgctatacgc acactgaaaa
     2221 aatgaaacat gcaaaatcag ttaaagatgt tgaagcatta agtgaatcac taacgaacgc
     2281 tttaaacctc gttaagaagg caaaattgtt atttgaattt caaaccgact accacaatga
     2341 gttgcttgct caatctgaaa ttttagatta attgaaacag gagaagagaa agatgaaaca
     2401 aacacaaaca gaaaccgtta acgatacgct tgctgatcaa ttagcaggtt attataaatt
     2461 atttgagatg ggtaaagcac agcgcttgat ccactatctt tggaaataca aaattttgtt
     2521 tcttttttgg gaatcaacac taacattctc agttcttttt tataagtcag atttttataa
     2581 tcaacactta gttttaggga gttttttgct tatctacacc attttgatgc ttaatttata
     2641 tacctacatc acacttgaag aacacttagt aattagcgat gcagggatcg agagaaacat
     2701 caaaagggta tccttcaaga tcacgttaaa caagcctacc gataaaaagc tctcagaagc
     2761 gttctgagag cttttgacat tttcttgtat aattttatcc taacacatta caaagcacct
     2821 aattcgagct tatatcaagc aaatttgcta tttattactc aatacatatc taaaaattac
     2881 ttgatggaaa attagataca tggtaagcat gaaaagaaaa tcaatttgag cagatgcagt
     2941 acaatacata gcaatttaaa agctctcaga aggcttctga gagcttttaa tatattcttt
     3001 gataatttgt ct
//
LOCUS       DACGLH010000154         2965 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.164, whole genome shotgun sequence.
ACCESSION   DACGLH010000154 DACGLH010000000
VERSION     DACGLH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.164"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..439)
                     /locus_tag="IXT21_002941"
     CDS             complement(<1..439)
                     /locus_tag="IXT21_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870282.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIETSLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPK"
     gene            complement(1077..1334)
                     /locus_tag="IXT21_002942"
     CDS             complement(1077..1334)
                     /locus_tag="IXT21_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ4870283.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1327..1596)
                     /locus_tag="IXT21_002943"
     CDS             complement(1327..1596)
                     /locus_tag="IXT21_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4870284.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1727..1906)
                     /locus_tag="IXT21_002944"
     CDS             complement(1727..1906)
                     /locus_tag="IXT21_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870285.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(2287..>2965)
                     /locus_tag="IXT21_002945"
     CDS             complement(2287..>2965)
                     /locus_tag="IXT21_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870286.1"
                     /translation="EDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFIQRVEEQLYLE
                     SETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKYIMNVYLERSELVENFVSYT
                     ICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLTCGTESIVLMG
                     MDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQR
                     FERKKRQ"
BASE COUNT          895 a          565 c          397 g         1108 t
ORIGIN      
        1 ctttcggaaa ctcaatatta aagtaccctt taaacatgct ccctaataat ttgtctacag
       61 ggggagattg taaattctca aatattagag ttaaatcagg tttctcttgt tgataaaatt
      121 cctttacccc tatttcacag gctgtaatta tcgaaataaa cctatctcta gtatttgata
      181 tatttttact tcttttaagg agtataagag aaaaggggat actttcgtgt ccatgcatgt
      241 aattaactaa ttgttgagct tcagaagtta tatctctaat tatatagtta acggaattag
      301 gaagtgtctt taactctaat gaagtttcta tccaattact atcttctaat ctgtatatgc
      361 tcgaagtata atttgattcg tcagcaagca tcgctacatc ccaaacactg atcataaaat
      421 cttttaaacg ttcaatcatt tcagcaatat tgctaaaatc agctaatttt tctttagaat
      481 aaaaaaaaca tttatatcag aatcaataaa ttctattttt tttattacta tatcgtcatc
      541 attatcttga ccatttatgt ttattgttcc ctgagtgggg agtacttctt tcaaattcaa
      601 gttaaattta tattccatta cttctcctcc aatatataaa aattacttaa ctgtatccaa
      661 atttaatatg taaataaatc attaattttt agctaaattc tatgagaagt ttatttttgt
      721 tgtttttgct aattaactaa aactagtaca aaaaatttaa aatagattat taaacctatt
      781 gcttattcta ttcagtcata tatataagcg tcatccttta gtaactaaca tcccacacta
      841 caatagtatt ataacatatc tattcaactt caaattgttg aaatttaagc catttatatt
      901 tacttttcct caactttaga aatgatttta ctaaccttat atccgtaata cgcgtaacaa
      961 acacattcat taataacaat caacactaag ctatattagc tttttaaatg actagggtaa
     1021 ataaaataag gatcaaaaac ttttaagttt ctgacccttt ccttacttcc gattggttaa
     1081 tagtgatctt ttgcagaata aataaatatc gtttcatttt caactctata tatcagtcta
     1141 tgttcatctg taattcttct ggaccatttt ccagataaat catgctttaa tggctcaggt
     1201 tttcctaatc cagcaaaggg ggaacgatcg atatctttta ttaacttgtt aatctttttt
     1261 atattgcttt tgtttccttg ctcatgccaa taaagataat catcccaagc atcatcagac
     1321 caagccttaa tcatcagatt caacctcgat taagtcatgt gttttaaatg cacctttgga
     1381 gaattgttca tctcctcgac gaattttttc catgacgtaa ttattagaaa gtgttctcaa
     1441 cgtttcttgc atagaatcat aatctctttt tgataataca acaactgtat cttctacatc
     1501 tttacttgtt acaataagtg tttcagcatc ctcattaact tgtttcatat aactacgtaa
     1561 attttggcgg aaatttgaat aagctactgc ttccattcct ttcacccctt atttatttct
     1621 ataaaacaat tgtacattat attgtacaat taagcaaatg attaatattc taacttctta
     1681 taattaaaag tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg
     1741 ctggtacctc aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa
     1801 tcctggattc acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt
     1861 catccaacgt aacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct
     1921 ttcatcgaaa taaccctatg aaaaatatgg ctgcaatcat cttgaatagt agactcacaa
     1981 gggctaatac aaagcgccaa aagatcaaac aaaataccag tattgccaaa aatactacga
     2041 agtttaaatg cacagctgca gcatggcgca taagtatttc gccaacaaat aggactaaca
     2101 atagaggaat gattttattc atatggatct ccttctttct cttaggttat cgggtgttgc
     2161 ccgcttattt cgcaccacct aagcggcggg ggcggttgga gcgattggtg cgaaataaga
     2221 accattccct tagttactag gttttttgtc cttatttcac acattctcac ttttcggtat
     2281 aaatgattat tgcctttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag
     2341 ttcattttgg atttcttctg tatcgtcgta agtataggtg tctaaaaaat cagttaaagt
     2401 cgtgtaccag tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc
     2461 gggtacagcg agttcataat ccattcccat taacacgata gattccgttc cacacgtcaa
     2521 aaatctagta aatacaaggt aataatcgtc tatcgtcgca tctttgctgt ttagctcttc
     2581 catttcttct tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta
     2641 atagtgtttc ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag
     2701 gtaaacattc atgatgtatt tttctgtttc tagtcgtaga agctccgttc ctttttttac
     2761 cgtttcagga cgacgtttat acacgtctaa acgattatgg ctgactgtga ccttcgtttg
     2821 actcgtttca ctttccaaat acaattgttc ttcgactctt tgaataaagt acttcatttc
     2881 ttccatacaa ctaaagtctt tttgaatccg tctgattact tcattcaaat gttctttgtt
     2941 tgttaaaaag tgtgacacat cttcc
//
LOCUS       DACGLH010000155         2952 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.165, whole genome shotgun sequence.
ACCESSION   DACGLH010000155 DACGLH010000000
VERSION     DACGLH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.165"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            89..406
                     /locus_tag="IXT21_002946"
     CDS             89..406
                     /locus_tag="IXT21_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ4870287.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="IXT21_002947"
     CDS             complement(497..862)
                     /locus_tag="IXT21_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870288.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="IXT21_002948"
     CDS             1070..1552
                     /locus_tag="IXT21_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870289.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /gene="pstS"
                     /locus_tag="IXT21_002949"
     CDS             1749..2639
                     /gene="pstS"
                     /locus_tag="IXT21_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ4870290.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          918 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tg
//
LOCUS       DACGLH010000156         2949 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.167, whole genome shotgun sequence.
ACCESSION   DACGLH010000156 DACGLH010000000
VERSION     DACGLH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.167"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..1259
                     /locus_tag="IXT21_002950"
     CDS             <1..1259
                     /locus_tag="IXT21_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4870291.1"
                     /translation="VIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGT
                     VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEV
                     TDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKK
                     GTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDL
                     PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITE
                     GDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGN
                     TSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNI
                     FTISGGLILLGTLGLLGYKNRKKENE"
     gene            1393..2736
                     /locus_tag="IXT21_002951"
     CDS             1393..2736
                     /locus_tag="IXT21_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4870292.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1170 a          397 c          554 g          828 t
ORIGIN      
        1 gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt ccagtagaag
       61 tgacagacaa tcgttctgac gccgataaat atgaaccaac agtagaaggc gaaaaagtag
      121 aaattggagg cacagtagat ttaacagata atgttactaa cttgccagcg ttaccagaag
      181 gaacaacagt aacagatgtt actcccggcg gtacaattga taccaacaca ccaggcaatt
      241 atgaaggtgt cattgaagta acgtatccag acggtacgaa agatacggtg aaagttccag
      301 tagaagtgac agacaatcgt tctaatgcag ataaatacaa tcctgaagga caaaaagtga
      361 ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatttac
      421 caaaaggaac tacttatgct tggaaagaga aagtagatgt tagcacagct ggaaataaaa
      481 aaggtactat tgtagtgaca tatccagatg gatcatctga tgaagttgaa gtagacgtta
      541 cagtgacaga tactcgttcc gatgcagata aatataatcc tgaaggacaa aaagtgacta
      601 cagacttaaa taaagagcca gatgcatctg agggaattaa gaacaaagaa gatctaccaa
      661 aaggaactac ttatacttgg aaagaaaaag tagatgttag tacagctgga aacaaaaaag
      721 gtactgtagt agtaacatat ccagatggat ctaaagaaga agtagaagtt actatttctg
      781 tagtagataa aaaagcaccg aataaacctc aagttgatcc tattacagag ggtgaccaaa
      841 ttgttactgg taaaactgaa ccaaatgcag aggtgacagt tacattacca gacggaagtc
      901 aacaccatgg gacagctgat aaaaatggta attttacagt taaagttcct aaattagagg
      961 cagggacaaa agtgatagta actgcaactg atgaatctgg taatactagt gaacctacta
     1021 atgtagttgt ttcttccaac aaaaaagaca gtggaaaaaa cggtagtaaa ggtagtaaga
     1081 ctgacaatca aggtagcaac agcaatcaag acaaaaatag aggcaaaagc cagtcctcaa
     1141 aagtttttcc taagacggga gagagtgaca gtaatatctt tactattagc ggtggtttaa
     1201 tactgttagg aacgttagga ttattagggt ataaaaatcg aaaaaaagag aatgaataga
     1261 aaatcaattt cttaagtctt ctctgagaag aggaagaatc tttttcttcc tctttttgct
     1321 tttttatgat gggaaataag tgttagttaa agatgaatat actttttata gaaaggaaaa
     1381 gaatgtaatt aagtggaaaa aatagtaatt attggttcaa accattcagg gctagccgca
     1441 gctcgatttc taaacttaag cgattcaaat tatgaagtta ttctaatcga taaaaaatca
     1501 gaaattagtt acttggaatg tgggacatcg ttatttataa aaggagaagt aagtaattta
     1561 gaagatcttt tttatcaaaa tagaatgaca ctaaattctg agataaaaaa gttatttcta
     1621 gagacagaag ttgacaaaat tgatattcaa aataaaaaga ttagttgtgt aaaaaaagat
     1681 ggtacaagat tctctcaaga atatgacaaa gttatattag ctacaggttc aaagcaaatt
     1741 atgctagata taccggggaa taatttagct aatatttttt cgataaagaa ttttccaaat
     1801 gcaaaagaga tttttaaagc tttggatgat agtactatta aaaaagtagg aattattgga
     1861 gcaggatata ttggagttga aattgctgaa gccataagaa aaagagggaa agaagtatat
     1921 cttttcgatg ttgcagatag agtgttgtcg acttattacg accgtagttt ttcagataaa
     1981 gttgaagaaa tactatcaaa aaatggaata catctttgtc tatcagaaag tcctaaaaaa
     2041 tatttaggaa gaaaaaagat tgaaaaaata gtcactacaa gtggtaccca atactctatg
     2101 gatatggtcg tacaagctat aggatttcta tctaatagtc caattgggga aaaggaactc
     2161 ttgagagatg ttagtggagc atatctaact aatgaatacc agcaaacaaa tataaaagat
     2221 atatatgcta ttggggattg tgctacaact ttttttacgt ccattaatag aagtagtgtt
     2281 gatttttcta tttctaatgc tctaagaaca gcttatattg caactcaaca tattaacaat
     2341 caagatttta caccttctgg ttcccaattt acaaatggct tttctatatt taattacaat
     2401 ttttacgcag caggtttgaa tttaaaaaca gcacgattat taagtgagtc tatagattat
     2461 atagaaatag aagatttcat tagacctgct tttttgagaa aaaatagtcg tgtaaaatta
     2521 agaattagct ttgaaaaaaa tacaaagaga atagtaggtg tacaactttg tagtcaagaa
     2581 gatttatctg gactattatc aatgttttct cttgctatag aagaacaaat tacattagac
     2641 aaattgaaaa tgttagatta tttatttcat cctagtttta gccaacccta taattttatt
     2701 ttaaaggcag taacaggatt aaacattgaa acataatcgt tactaaatga aatcaagaaa
     2761 tatactgtat tactcaattc attgaaaata tttatcataa tttttaatag aaaacactat
     2821 taaagagaaa aaagagaggt aaatcaaatg aaaaaatctt taaaacttgc atcagcagtt
     2881 atattaatgt cccaactcct tatagcaaca cctttccatg tgttggcaag cgaaaattca
     2941 acatacaaa
//
LOCUS       DACGLH010000157         2947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.168, whole genome shotgun sequence.
ACCESSION   DACGLH010000157 DACGLH010000000
VERSION     DACGLH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.168"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(449..865)
                     /locus_tag="IXT21_002952"
     CDS             complement(449..865)
                     /locus_tag="IXT21_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870293.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="IXT21_002953"
     CDS             complement(867..1148)
                     /locus_tag="IXT21_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870294.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="IXT21_002954"
     CDS             1948..2922
                     /locus_tag="IXT21_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ4870295.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DACGLH010000158         2900 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.169, whole genome shotgun sequence.
ACCESSION   DACGLH010000158 DACGLH010000000
VERSION     DACGLH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.169"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..811
                     /locus_tag="IXT21_002955"
     CDS             <1..811
                     /locus_tag="IXT21_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4870296.1"
                     /translation="QIRDIAMIFQDPMTSLDPTMPIGKQVAESLRKHNKVSKKEGQKA
                     ALELLKLVGIPEAEKRINSYPHQFSGGQRQRIVIAIALVCYPEILIADEPTTALDVTI
                     QAQILELLKEIQTKIDTSIIFITHDLGVVANVADRVAVMYGGRIVEVGTSEEIFYNPQ
                     HPYTWGLLGSMPTLDSANDRLYAIPGSPPDLLNPPKGDAFYPRNEFAMKIDAEQEPPF
                     FELSKTHKAATWLLAPQAPKVTPPEEIQRRWAIFKEKQKHTYGQGGIADAK"
     gene            801..1754
                     /locus_tag="IXT21_002956"
     CDS             801..1754
                     /locus_tag="IXT21_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4870297.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            1766..2776
                     /locus_tag="IXT21_002957"
     CDS             1766..2776
                     /locus_tag="IXT21_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4870298.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
BASE COUNT         1013 a          553 c          584 g          750 t
ORIGIN      
        1 tcagataagg gacatagcga tgattttcca agatccaatg acctctttag atccgacgat
       61 gcccattggg aagcaagtcg ctgagtccct tagaaagcat aataaagttt caaaaaaaga
      121 gggacaaaaa gctgcattag aattgcttaa attagttgga atacccgaag ctgagaaacg
      181 tatcaacagt tatccgcatc agttctcagg aggtcagcgt caacggatcg tgatcgcgat
      241 tgcactggta tgttatccgg aaatcctgat tgctgatgaa cctacaacag cgcttgatgt
      301 gactattcaa gcacagattt tggagctgct gaaagaaatt cagacaaaaa tcgatacatc
      361 aattatcttt atcactcatg accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt
      421 catgtatgga ggaagaatcg ttgaagttgg aacatcggaa gaaattttct acaacccgca
      481 acatccctat acttggggac tgcttggttc aatgccgaca ttagatagtg cgaacgatcg
      541 attgtatgct attcctggtt ctccaccgga tttattgaac ccgccaaaag gagacgcatt
      601 ctatccaaga aatgaattcg cgatgaaaat cgatgcagaa caagaaccac cattttttga
      661 actatcaaaa acacacaagg cagcaacttg gctacttgct ccgcaggcac ccaaagtgac
      721 accgccagaa gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa aacatactta
      781 cggtcaagga ggaattgctg atgccaaata aactacttga agttaaagga ttgaaacagt
      841 atttcaatgt aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc tttcacattt
      901 atgaaggaga aacattcggt ttagttggag aatctggaag cggaaaatcc actactggcc
      961 gtacgatcat tcgcttgaat gaacctactg atggtgagat attattcgac ggtcaagata
     1021 taacgaagat caaagataaa aaaggtttga cgaaattccg gcatgatgtt cagatgatct
     1081 ttcaagatcc atacgcttcg ttgaatcctc gtatgaaagt cagagatatc attgccgaag
     1141 gaattgatgt taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc
     1201 tattgagaac tgttggattg aatccaagcc acggtactcg ttatcctcat gaattttcag
     1261 gtgggcaacg ccagcgtatt ggtattgcac gagcattggc agtaaaaccg cgttttatca
     1321 tttgtgatga accaatttct gcactagatg tgtcgatcca agcccaagtg gtcaatttgt
     1381 tgcaagatct ccaaaaagaa catcaattga cctatctatt tattgcacat gatttgtcga
     1441 tggttaaaca tattagtgac cgaatcggtg taatgcataa cggcttgtta ttagagatgg
     1501 gaacaagtga tgaaatctat aatcacggtg ttcatcctta tacagaaagt ttgttatctg
     1561 ctattcctct accagatcca gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg
     1621 aaccagatga cggacaagta cgtcaacttc gtgaaatcgc accagaacat tttgtctatg
     1681 caactgagca ggaagtccca tattacgcaa aaaaactaaa acgacaaaag gaatctcttc
     1741 tggttgctaa ctaggaggga catagatgct aaaagctttt aaattccgta tgtatccgac
     1801 agaagaacaa aaacagcaat tgattcgtac ttttggatgt gctcgattta cctataacca
     1861 tttgctgaaa aagcggcaga aaagttggca acaaacagga gtagccaatt tttcgctcac
     1921 acccgctaca ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc ttgctttagc
     1981 aaatgcacag cttaatttgg atcgggcttt tcgcaattat ttcaagggac gtgccagctt
     2041 cccaaagctt aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca accaaacccg
     2101 tacgatctat ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac taatcaagat
     2161 aaatcagcat cggccagttg aaggatcgat tcgctccgct acgatttctg ctcgatataa
     2221 tgaagaattt tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag aatctttagc
     2281 taaatggatc gggatcgcct atcatccgaa gacattgatc caaacatctc gacctctaga
     2341 agtaacttta ccaaaatttc accagacaga agaaaagctg caacatgcac agcggaaatt
     2401 gaatgtgaaa gtccgctccg ctcaccatag aaagatcagg ttagatcagg caagcaatta
     2461 tcagaaacaa aagagaaaag taatggatct ttatctgaaa caaaagaacc agcgagaaga
     2521 ttatcttgaa caattatctg ggaaattggt caaacaatat gactacttat ttgtagaatc
     2581 ttttccaaaa gaagaggcac atgctgattt ttctattcac gactggcata aattaataac
     2641 aaaattgcgt tataagtccc aatggtataa taaaaaattt cttttgatca ataccgacgg
     2701 agcagaagaa agcaattccg taagaaagag ccaagtagta gaaaaaatgg gcagacattc
     2761 ggttatcaag ggataaaaaa tgaatgaaac tgataggagc gttagggtta gccttgttaa
     2821 taaaaagtct taatgacttt tgtttctagg aatttttctt ttgaagaaaa gctcactaat
     2881 tatgacaaac caataaaaca
//
LOCUS       DACGLH010000159         2867 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.170, whole genome shotgun sequence.
ACCESSION   DACGLH010000159 DACGLH010000000
VERSION     DACGLH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.170"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            256..1224
                     /locus_tag="IXT21_002958"
     CDS             256..1224
                     /locus_tag="IXT21_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ4870299.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1217..2248
                     /gene="vanA"
                     /locus_tag="IXT21_002959"
     CDS             1217..2248
                     /gene="vanA"
                     /locus_tag="IXT21_002959"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ4870300.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2254..2862
                     /locus_tag="IXT21_002960"
     CDS             2254..2862
                     /locus_tag="IXT21_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ4870301.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          845 a          549 c          702 g          771 t
ORIGIN      
        1 ttccagcgat gccagacttg gttgataaaa ggaggtccta agagatgtat ataatttttt
       61 aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa
      121 acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag
      181 agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa
      241 gggttggtct taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag
      301 gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc
      361 aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat
      421 aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt
      481 tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc
      541 actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt
      601 cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg
      661 ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc
      721 cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat
      781 agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc
      841 gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa
      901 caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta
      961 gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat
     1021 gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat
     1081 caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat
     1141 tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt
     1201 gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga
     1261 ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa
     1321 atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc
     1381 ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat
     1441 gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt
     1501 ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc
     1561 cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt
     1621 gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa
     1681 agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc
     1741 aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga
     1801 atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt
     1861 cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg accaaatcag
     1921 gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa
     1981 cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac
     2041 ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt
     2101 acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata
     2161 cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg
     2221 cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat
     2281 gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga
     2341 aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg
     2401 cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt
     2461 taccgtccta agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac
     2521 ctgacaaagg aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac
     2581 gtggcttcaa aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta
     2641 gacacgggtg agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat
     2701 catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc
     2761 atggaaaaca gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac
     2821 gaaccatacc ccaatagcta ttttgatttc cccgttaaat aaacttt
//
LOCUS       DACGLH010000160         2811 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.171, whole genome shotgun sequence.
ACCESSION   DACGLH010000160 DACGLH010000000
VERSION     DACGLH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.171"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(2..301)
                     /locus_tag="IXT21_002961"
     CDS             complement(2..301)
                     /locus_tag="IXT21_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870302.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(307..537)
                     /locus_tag="IXT21_002962"
     CDS             complement(307..537)
                     /locus_tag="IXT21_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870303.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENNQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            1154..1816
                     /locus_tag="IXT21_002963"
     CDS             1154..1816
                     /locus_tag="IXT21_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870304.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1895..>2811
                     /gene="ecbA"
                     /locus_tag="IXT21_002964"
     CDS             1895..>2811
                     /gene="ecbA"
                     /locus_tag="IXT21_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family collagen-binding adhesin EcbA"
                     /protein_id="HAQ4870305.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKT"
BASE COUNT          982 a          461 c          518 g          850 t
ORIGIN      
        1 tttaatcttt atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat
       61 gatcgcttcg tgcgtctggc acgtcagtca cgatatcaaa caaaaaatac acatctttct
      121 tcattctggt tttcgcagca gggattttaa agtagttctt attagaatag tagagattga
      181 ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat
      241 ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca ttaaaaagca
      301 taaccattac tccgatctat ttttaaactt agtttctacc tctaatatat atcgagtttt
      361 tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttggt tattttcgga
      421 aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt cgttgctttg
      481 ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt ccatcataaa
      541 gatcacctca aaacgattat aggaacttac gttcttgttg taaagcgata tttgaggggc
      601 aaaaaggggg caaaaatgcg tttaactttg atatatctta gattgaacat agtgtttatt
      661 ttatgtcggt attgttgatt tatagctgtt ttgagggtgt cagatacata atagaatata
      721 gtggttttgt ttacgtgcat tacacggaaa agctgctcgt atcaaagaaa tcagaagata
      781 aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa gtccttgtat atcaagggct
      841 ttttcttttt tctctatcac agttttaagc catttttggc tgttttggac catttttcaa
      901 tcatattccc ttaaaaattg tcctagattg tccgacattg actatcagtg tcgtcaaaat
      961 agggcaaaat ttagtagtca gcttgacgac aaatataata cgaaaaatac caaaacattt
     1021 accgaattta gcaacttttg aacccaagtt tctcaaaggg tcagctatta gcaaaaaaga
     1081 caaaacatgt acttttctac taaaaagttg atgtgatagg cttctatcta aataactatt
     1141 aggagttttt atcgtgaagc aaaatgatgg acgtatcatt tggaaaaatc aaagtgagtt
     1201 aaaactgatt ttaacaatta atgaattcat cgaaaaacac ggaattacat ctagccgtca
     1261 ataccagaaa aagctatcag aaaatccaaa ttctgcacca agtatgtggt tcattaacaa
     1321 aaaatatgga tcatgggaga atttattgat tagtatagga cgagaaaata ctgggtatgg
     1381 aaaatgggct agaatgtcag aacaagaatt attggaaata gtagaagcct ttattaagtg
     1441 cgagaagata acctctcaac gaatgtacga acaaaaatca gtagggaaga atattccttc
     1501 acttagtacc attaaaaaga tgttaggaga tatacgtcca ctttttaaag aaaaaaatga
     1561 tggatctcga tttactgatt tcgaactgct gttagaacta aaaaatgaaa taatccgatt
     1621 aaaactacag gatgatttat caatgacaaa gtttcgaaaa ctagttcaat ccccaaaact
     1681 accatcagtt gatacaatta tgaaaagaac gaacaaaaat tgggaggaat tgatggcaga
     1741 gattggattt gattatcgaa gaatcaaaat ttataagcag agaaacaacc tatccaagac
     1801 aaagaaaact aaatagtatc actctaaatc agacgttcaa aattaaaatt tggacgtctt
     1861 ttttattacc caaaaacgaa aggagattta ttttatgaaa aagccaagat ttaagaattg
     1921 gcgtttgctc gcaacactga gtctattatg ccaaacaatt ggtggatcgc ttggcccaac
     1981 gattgccttt gctgatgaaa ttactcatcc acaaacggta actgtggaat tggatttggc
     2041 tcaccagtat gctgtcgaag gtacatttag cgatggtcgt cccatgtcag aagttaccgt
     2101 tccgcactat gcagtttaca atggtgtgaa gcaagatatt ttctgtattg agccaggggt
     2161 gccgatttac aatgagttca ctcctggtta tgagaaaaat ccattgcctg atatgtcgga
     2221 gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt acggatgtgg atacacatat
     2281 tgtggcccag aagatgattt ggcaagaagt taatggctac acgcttcact caattaaacg
     2341 ttctgatggt agtgcagtaa atatcgcagc cattgagtca aaaatcaatc aagccattgc
     2401 ggattatcaa aagaaaccaa gcttccataa ctccacagtg aaaaccgtgt taggtcaatc
     2461 gactactgtg acagatacga acggcttgaa tttatcggag tttgatgaag ttgtcgagaa
     2521 tatcgcaaat attgattatc gtgtgaacgg caatcaacta gtgattactc caaatgccaa
     2581 ttcaaaagag agtggtgaat taaccctaaa gaagtcagct aatacaggaa caccggtagc
     2641 ttataaaatg gctggtcaac aaactttgat ggcgggggca attgataagc ccaataccta
     2701 cacaatcaaa attgatgtgg aaactgaggg ttctttgaag attaaaaaag tcgataaaga
     2761 aacaggaaat gttgtaccgg gaaccgtttt ccatttagac tttggaaaaa c
//
LOCUS       DACGLH010000161         2719 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.172, whole genome shotgun sequence.
ACCESSION   DACGLH010000161 DACGLH010000000
VERSION     DACGLH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.172"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IXT21_002965"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IXT21_002965"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ4870306.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DACGLH010000162         2634 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.173, whole genome shotgun sequence.
ACCESSION   DACGLH010000162 DACGLH010000000
VERSION     DACGLH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.173"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            5..1150
                     /locus_tag="IXT21_002966"
     CDS             5..1150
                     /locus_tag="IXT21_002966"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ4870307.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IXT21_002967"
     CDS             1321..1779
                     /locus_tag="IXT21_002967"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ4870308.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IXT21_002968"
     CDS             1938..2408
                     /locus_tag="IXT21_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ4870309.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IXT21_002969"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IXT21_002969"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          863 a          504 c          496 g          771 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DACGLH010000163         2593 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.174, whole genome shotgun sequence.
ACCESSION   DACGLH010000163 DACGLH010000000
VERSION     DACGLH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.174"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..1152
                     /locus_tag="IXT21_002970"
     CDS             <1..1152
                     /locus_tag="IXT21_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870310.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IXT21_002971"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IXT21_002971"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ4870311.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          859 a          491 c          620 g          623 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca act
//
LOCUS       DACGLH010000164         2515 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.175, whole genome shotgun sequence.
ACCESSION   DACGLH010000164 DACGLH010000000
VERSION     DACGLH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.175"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(493..1785)
                     /locus_tag="IXT21_002972"
     CDS             complement(493..1785)
                     /locus_tag="IXT21_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ4870312.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IXT21_002973"
     CDS             complement(2059..2319)
                     /locus_tag="IXT21_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870313.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          847 a          463 c          380 g          825 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagtgt aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aaggc
//
LOCUS       DACGLH010000165         2483 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.176, whole genome shotgun sequence.
ACCESSION   DACGLH010000165 DACGLH010000000
VERSION     DACGLH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.176"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(1013..1303)
                     /locus_tag="IXT21_002974"
     CDS             complement(1013..1303)
                     /locus_tag="IXT21_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870314.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1511..2203)
                     /locus_tag="IXT21_002975"
     CDS             complement(1511..2203)
                     /locus_tag="IXT21_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ4870315.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          733 a          458 c          349 g          943 t
ORIGIN      
        1 tacttgcaaa gtaatactac aattcctaac ataatcgtag aatttaataa tttccatagc
       61 ataaagtgtt tagaaaagaa cattaagaat agttctataa taattcttga actccatgtc
      121 ttataatgtt ctatcgaagc acccaaatag ccaagttcat ctaatctact tgcataaact
      181 acgtcatccg ctctcatagt cggcatatac atattaaaga gtaaaaagat accccaaatc
      241 cctattagta acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt
      301 tttatactcc ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag
      361 ataacttaaa agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga
      421 aatctacgat agtcaacaaa aaaattttca ttatatccag aaaaattttt cttagttaag
      481 gacctatgga ttgcagtaat gcagaattcg tcgaataaac tgccaaagac atacaatcat
      541 attgctttta aaattgacga acaagatata gattcgtttg tctcaaaaat ccaaatgtta
      601 ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg aggcttcttc aatttacttt
      661 tacgactatg ataatcacct cttcgagtta cataccggta ctctccagga acgtttaaaa
      721 tcctacaatt ccactacctg agactagtaa tgactagtct ctttttatgg cctctgatcc
      781 ccccattgag cgaatactaa tagggcttcg aattgatctg ttcaatcttt aagcaatatc
      841 cctcaaatga gtttggaaag tttagtgatt catcacaaat acttaaatac atgtagttat
      901 atatagattg aggttcttgt gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat
      961 ccatctttcc aaaacaagcg gctaggacat ttttgtctca gccgcttgtt ttctagttta
     1021 ttctttcatc ttgtcggacc ttttttcttg ggtatagact tttctcatat tctaccacaa
     1081 ttttgggttt ccctaacaca tgataggatc tcacaacaaa ttgctttcta ttattataat
     1141 aacctgtaac gactaacttc atcccttcgt ctacttcagc taggaagttc aaactatggg
     1201 aagcaatcaa acaattgacc ccatttaaac tgaaacggat caacggtctg gcactaactt
     1261 gcaaagtacg gatcttatct actgttccat attttttctc cataaccttc acctcaatac
     1321 cattatacga acatacattc cctttgtaaa gaagaaactc tgttttatct ttttgacttt
     1381 ttgtctatta cctttcttaa aatcatattg gcacttcctg cgaagatgaa ggacatgtac
     1441 taaaaaaaag aggcagcagt atagattact gttgcttctt tactaattta ggtttacttg
     1501 ttaagaaaga ttatttacgc aaatgttctt caacaatttg ctgagattta tcagctgtat
     1561 cttccacttc atcttttgct ttttctaact tttgaatata ttgtttcaag cttgagtttt
     1621 cagcagttgc ttcgtctaat ttcttttgtg tatctgttaa ctgagtttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACGLH010000166         2463 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.177, whole genome shotgun sequence.
ACCESSION   DACGLH010000166 DACGLH010000000
VERSION     DACGLH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.177"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            289..735
                     /locus_tag="IXT21_002976"
     CDS             289..735
                     /locus_tag="IXT21_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870316.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            737..874
                     /locus_tag="IXT21_002977"
     CDS             737..874
                     /locus_tag="IXT21_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ4870317.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            912..1205
                     /locus_tag="IXT21_002978"
     CDS             912..1205
                     /locus_tag="IXT21_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870318.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            1202..1426
                     /locus_tag="IXT21_002979"
     CDS             1202..1426
                     /locus_tag="IXT21_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ4870319.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            1423..2448
                     /locus_tag="IXT21_002980"
     CDS             1423..2448
                     /locus_tag="IXT21_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4870320.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPQR"
BASE COUNT          860 a          406 c          520 g          677 t
ORIGIN      
        1 ggctcgatag accgttctac tttgaacagt gctcagttat ctattggtgc agatggagcc
       61 attaaatggc gaagaaactc aagttatgga tcggattata cctttgcaat tacttacacg
      121 atttagaaaa gcgtgaatcg atatgaaggc agcatataga ccaattgaac cttacggatt
      181 cgagcaaatc attgtgaatg atgaagaaca tttaccggaa gaatgcacag aagtcgaacc
      241 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
      301 tggaaatatg gacgtactgg cggagagtac gcaggaaaag tattggacga catgcttgta
      361 tccgttcctt acacagatca gccaccgctt gaaggaattc gtgctgatgg cgaaccgtta
      421 acaatcgctg atcaaatgtt tgatcctaaa ttgaatcaat ggattatttt agcgaacgca
      481 ctagatcaca acgatttaaa caatctcaaa gcaatgtatg agtcgttaga aaatgagaac
      541 ggcgatttaa aacagatcaa tgccaaactc atgctaagcg atgtagcaat taaacaggaa
      601 aatactgcat tgaaagaaaa agcggatagt ttagcacaaa tcaattcaaa aatgatgctt
      661 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
      721 aagggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
      781 tttcactaac gaacaagtaa tggttttcgt tccgttgtgc attactgaag aaaaagcaga
      841 taaaatcatt agcaaagaag agagcgcatc ttaattgatg tgctttttat tttgattcaa
      901 ggagttgtca catgattaat ttaggggaat ggggagcgat agcaggatca ataaccgcta
      961 tcgtttcttt gattttatta gtaataaaac caattactgc atctttctcg aagattactg
     1021 agactctttc aaaagtaagt cacaatttag atttgctgac taaagattta gaatcgagca
     1081 aatcagatcg attgatgatt catgaagaac taaagaaaca cgatgaaaga ttagatacac
     1141 atgcagaaaa attggtagaa cacacgcaac aaattaaaac tttatttagg gagagaagaa
     1201 aatgaataat aaaacgttcg aagtactaaa atggttcgca ctggtaatta ttcccgcact
     1261 agctactttc gtggggttag ttggtaaagc gctcaattgg cagtacacag atatctgtgt
     1321 tgtcatcatt actggttttg gcgcgttttt agggagtgtg ttgggtgtat caaatcgaac
     1381 ctacaaaatg ttctcggctg aaagcgaaga aggaggaaac aaatgaaaaa gaaaattact
     1441 attactgcga tgagcctgtt aacggctctt tttttattac ccattaatac gtttgcttat
     1501 actattaatg acgagtataa tttagcgccg aatcaaggag actccagatt agcaattcct
     1561 aacaaaatta ttttgcacga aactggaata gatgcaccag caagaaacgt agccgccaac
     1621 atgaaaaata attataacgg aagcaattct tatactacag atgttattgg tgacggtggg
     1681 attgtttacc gtgtgggtga gcaaggatat gtttcgtggg gagctggtaa cgctaatcct
     1741 tatgcgcctg tacagattga attacagcgc acatatgata aagcattgtt tgaaaaaaac
     1801 tatcgagctt atattgaata tacaagagat agtgcaaaaa aatatggaat tccattgact
     1861 cttgatcaag gaacttcttt atttacaaaa ggaatcattt ctcatttgtg ggtgacaaat
     1921 tatgtttggg ggaaccacac agatccatat ggttacttat cgcaaatggg agttagcaaa
     1981 gaaaagcttg cttatgattt agctcatgga tttaccgatg aaaatccaac aacttctgaa
     2041 aacaagcctg tcattgatcc aacacgagct ggtgcagcta atcctacgct gacagatgga
     2101 acaaatcacg cccacattga tcagtttggg gaaatcgaaa acgcaaactt gcatgtggct
     2161 ggatggcaca ttgctaacta taaatacgag tatattttca ttatggacta caatactggg
     2221 aaagaattag ctcgagtaag agctgatgga atttatagac cagatgtaaa ccaagcttat
     2281 aatacttctg gaaatgttgg ttatcatgta tctttcaata tgcgtaattt tcctagtaag
     2341 aaagtctatg taatgatgcg ggcaacgaat gatccagagg gaaacactaa aggcggtgcg
     2401 caagatttcc atgacaaacg ctggtattta aatattccac aacgataaaa aatagcccct
     2461 cga
//
LOCUS       DACGLH010000167         2439 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.178, whole genome shotgun sequence.
ACCESSION   DACGLH010000167 DACGLH010000000
VERSION     DACGLH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.178"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            950..1423
                     /locus_tag="IXT21_002981"
     CDS             950..1423
                     /locus_tag="IXT21_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ4870321.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="IXT21_002982"
     CDS             1432..1659
                     /locus_tag="IXT21_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4870322.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(2093..>2439)
                     /locus_tag="IXT21_002983"
     CDS             complement(2093..>2439)
                     /locus_tag="IXT21_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAQ4870323.1"
                     /translation="KQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKL
                     ATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCHYYQNNVKNKKELSITV
                     IQIILSKLHSKC"
BASE COUNT          774 a          409 c          362 g          894 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgacagactg tagacaaaag ctgttccaaa ctcgtaactc
     2221 gagcttggaa cagcttttta tctacttcgt ttcgaatttt tgaacaaaaa aataaaggct
     2281 ccttgccttt ccatagccag gtggctaatt tctttaaatt cattgcagca aacactagca
     2341 tcgtatgcgt cgcgactttt tccaagcctc gatacttggt ccaacgcatc ccatgctttt
     2401 cttttgcatc cgcaaatatt cgttcaatcg tttgtttcc
//
LOCUS       DACGLH010000168         2397 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.179, whole genome shotgun sequence.
ACCESSION   DACGLH010000168 DACGLH010000000
VERSION     DACGLH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.179"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            206..2068
                     /locus_tag="IXT21_002984"
     CDS             206..2068
                     /locus_tag="IXT21_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4870324.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IXT21_002985"
     CDS             2068..>2397
                     /locus_tag="IXT21_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4870325.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACGLH010000169         2313 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.180, whole genome shotgun sequence.
ACCESSION   DACGLH010000169 DACGLH010000000
VERSION     DACGLH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.180"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            191..445
                     /locus_tag="IXT21_002986"
     CDS             191..445
                     /locus_tag="IXT21_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4870326.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IXT21_002987"
     CDS             446..763
                     /locus_tag="IXT21_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4870327.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="IXT21_002988"
     CDS             1322..2257
                     /locus_tag="IXT21_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870328.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          846 a          313 c          434 g          720 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag aaaaatgtct
     2281 aaacatgttg gtaaatcaac aaaaaaacgc ata
//
LOCUS       DACGLH010000170         2136 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.181, whole genome shotgun sequence.
ACCESSION   DACGLH010000170 DACGLH010000000
VERSION     DACGLH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.181"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(449..522)
                     /locus_tag="IXT21_002989"
     tRNA            complement(449..522)
                     /locus_tag="IXT21_002989"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(486..488),aa:Ile,seq:cat)
     gene            complement(711..1730)
                     /locus_tag="IXT21_002990"
     CDS             complement(711..1730)
                     /locus_tag="IXT21_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4870329.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1741..1947)
                     /locus_tag="IXT21_002991"
     CDS             complement(1741..1947)
                     /locus_tag="IXT21_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ4870330.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          644 a          424 c          348 g          720 t
ORIGIN      
        1 ttttaagtta gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc
       61 tataaaatca aaaataagtc actaattaac taccactcca attgtaagcc ttttttctca
      121 ctttttttca aaaatatggt atgcctttta atagcttcaa atataaaaga gtttaaagcg
      181 taacacactt atggggaagt ggcttggggt gcgctttaaa ctcttcttta ttatcatctc
      241 acaatttaac ccaaatgtct ttctatttaa aaatcaaagt aaaacttttc aaatatacag
      301 aaatataact atgtgaaaca tcctttcatt aatccataaa aggatacata aaaaagccac
      361 tcatttgagt ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaattctaa
      421 cacagaacga ttcaaatggc tacgttaatg taccctgtag gactcgaacc tacgaccgga
      481 cggttatgag ccgtctgctc tgaccaactg agctaagggt actggttgtt gccacataaa
      541 gccataaaca atcaaccagt agaatgtgtg gcaacaaacc tgttatcgca tagcttggag
      601 tgtgactatt atgggtgata gtgaagatat gcgataacat cactatttta tcgaatgatt
      661 tttatagttg tcaatatagt tatgtaatgc tcctcaacga ggagctattt ttatcgttta
      721 ggaatattta aataccaacg tttgtcatgg aaatcttgcg caccgccttt agtgtttccc
      781 tctggatcat tcgttgcccg catcatgaca tagactttct tattagggaa gttacgcatg
      841 ttaaaagata catgatagcc aacatttcca gaagtattat aagcttgatt tacatctgat
      901 ctataaattc catcagctct tactcgagct aattctttcc cagtattgta atccataatg
      961 aagatatact cgtatttata gttagcaatg tgccatccag ctacatgcaa atttgcattt
     1021 tcgatttctc caaactgatc aatgtgggcg taattcgttc catctgtcag tgtaggatta
     1081 gctgcaccag ctcgtgttgg atcaatgaca ggcttgtttt cagaagttgt tggattttca
     1141 tcggtaaatc catgagctaa atcatatgct aatttttctt tacttacgcc catttcagaa
     1201 agataaccgt aaggatctgt atgatcgccc cagatgtttt gtgttaccca taaatgcgat
     1261 ttgattcccg gttggttata aggagtgtcc aacgttaatg gaataccata tttcattgct
     1321 gaatctctag ccaattcaac gtatgccttg tagtttttct caaacgttgc tttatcatgt
     1381 gtgtgttgta actcaatctg cacaggactg ttggcattag catacgaacc agcaccgtac
     1441 tgtacataac caggttgacc gacttgataa acaattccgc cgtctcccac aatataagca
     1501 gtataagcgc tagtccatga acgttgcata tactgcgctt cattgcgtcc tgttgctgtt
     1561 tcattagccg tttcatgcag taaaatatac tgattatttg ctacttgtga gctaccttca
     1621 tttgcgccca aattaaattc attgttgata gtataggcaa atccgttaat tggcaataaa
     1681 aaaagagccg ttaacaggct catcgcagta atagtaattt tctttttcat atctatcctc
     1741 ctatttatct gatgaaattt tcttatacgt tcgatttgat acacctaaaa tactccctag
     1801 aaatgctcca aaacctgtaa taatcatcac cgttatatct gtgtaccgcc agttaacacc
     1861 ttttccgaca agaccgacaa aagtcgctaa agcggggata actactatag cgatccattt
     1921 caaaatatca aaggttctat tgttcatact atccacctcc cttcattggt tgggctactt
     1981 tctttttcaa tcgcttgttt tcttctatca atatttctct ttctgaaagc aaggcatcaa
     2041 tttgcccgtc cagttgttca attttatctt tcttaattgt caaattaagt tagacagatc
     2101 atcaatactt acgtaactca aaaatacttc caaagg
//
LOCUS       DACGLH010000171         1933 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.183, whole genome shotgun sequence.
ACCESSION   DACGLH010000171 DACGLH010000000
VERSION     DACGLH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.183"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(209..694)
                     /gene="vanZ"
                     /locus_tag="IXT21_002992"
     CDS             complement(209..694)
                     /gene="vanZ"
                     /locus_tag="IXT21_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ4870331.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(847..1758)
                     /locus_tag="IXT21_002993"
     CDS             complement(847..1758)
                     /locus_tag="IXT21_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ4870332.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          606 a          374 c          264 g          689 t
ORIGIN      
        1 tttattaaag agaccaattc ctcagcacta aaattgataa ttgggctttc tttgttacta
       61 tagtgttagg taagcttttt actttatcac cggttttccc ctcacttcac acctacgggc
      121 gagtttcccc acatcccgtt ttccatctaa tccaattcat tcaatatctt tatgtgtatg
      181 aaagatcaga ttgcttgatt tagacatctt acacgtaatt tattcttaaa tgggtacggt
      241 aaacgagcaa taatacgagc aaaagtatac ctacaaaaat aataactctg tctaattttt
      301 tttgattcat atgcttattg cttaaaccat ataatttcag tccaagaaag cctccaacag
      361 tatttgtaat tacatctgtt atgtctgtcg ctccaatagc gaagataaat tgaattattt
      421 caaaagtaag acttaaaacc agtacaaaag caaacttagg taaaaatccg atttctttaa
      481 aattgacatt caaaagcaag ccaaatggaa taaagattat aacattatct atcatctctc
      541 tgaaattccc agtagcagta aatggagtca agttaagact tctttgatga taattaaata
      601 ctgataaaat attgtattgt aatttgaata acactaacca gattagtgtc actaaatata
      661 aagctagcaa tcctctagat aatatttttc ccaatataat ttcctctaat tcttttgttt
      721 aagttgtatc attagtatag caatgtccta gttaacgtgg gttttaaatt aaaaagctaa
      781 cgttacccca aaagtgctag tacaataatg ctgttcatta atttcgttgg tttcattccg
      841 ccatccttac ctccttgaat tagtatgtgt tgatccggga aacactgtca caattacacc
      901 gtctatattg tttcctgata tctcataacg aagattagtc ggcacatgaa tggtggtatt
      961 tttagtaaca ggataataaa agatctcata tttttcccca tttacactaa cagaaatggt
     1021 tttttcttct tttaggtaat ccatatattc ctcgagaacg aaattctttt ctttcataat
     1081 cgcactatgt ggtaaaccaa catagcgaat atgccatggt tcatattgaa ttcctgttaa
     1141 ctctgttttg tcctctggat aacgtaaaat gaacccgtat ttccaagcat tttcttctat
     1201 ccactttcct tcaggggctc gttccatttt cgtcaagctt gatcctacat ctagtgataa
     1261 acctgaatta tgctcactat aacctgctgg taaggcatac tcagccccca tttcttggta
     1321 aagcacactt tgctcatcaa agtctcgata gccactatta ataataaaat gactaacgcc
     1381 accctttaca gcatcattga ccatctctga aaatttttgt gctatttctt ttgacatata
     1441 aatattacta tcaagcaacc cgtatccatt tattaattcg tcatgtttag ataaattcac
     1501 gatatctgac ttcacacttt cttggcgaac aggatattta ctattgatta atagcagatt
     1561 tccttgataa acctgttctt ctgtaattgt tttctcttgg gtattttcag aagtcccact
     1621 attttctaaa tgttctttgg gattttgatc ataattttga aattcgactt tttcctgaga
     1681 aaacagtgct tcattaacgt agtcataacc taagtatatt aagaataaca ataacaataa
     1741 aaaaaacaac ttcttcattt tcagtctcct ccttaactat atagttggaa gtatgaaata
     1801 tttctagaat cttcccctta aacggcacaa tctcgtttga gattgtgccg ttttgccccg
     1861 taaattacag tcaattattt cgccctactg gatttactat tatcaccaat gtagatgaat
     1921 tttcataagt gat
//
LOCUS       DACGLH010000172         1901 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.184, whole genome shotgun sequence.
ACCESSION   DACGLH010000172 DACGLH010000000
VERSION     DACGLH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.184"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(1..673)
                     /locus_tag="IXT21_002994"
                     /pseudo
     CDS             complement(1..673)
                     /locus_tag="IXT21_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            751..1790
                     /locus_tag="IXT21_002995"
                     /pseudo
     CDS             751..1790
                     /locus_tag="IXT21_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          553 a          403 c          398 g          547 t
ORIGIN      
        1 cgggaattct aaattcagta atatctgtta accatttttc atttggcttc tttgctttaa
       61 aattccgatt taataaattg gggtgtgcag gagaaatttc cccagcatac gaagaatact
      121 tttttcgtct agaaaattta acaaatagat tttcctcagt catcaatcta cgtacaactt
      181 tttcagaaag ctttttaccc atatttttta aagcttggtg aattctacgg taaccatacg
      241 cacagaagtt atgattgaaa atcgtgataa ttaattgacg ttctttgtaa tacttatctg
      301 gtttctcaag tgcttgtttt tgataaaaat agctgctttt aggcaactgg agctgttgaa
      361 gaagctcttt caaggtgaac tgatttcgta aggcatcaat caatatcgtt ttttcttgat
      421 ttgatagaca aagaagattg acgccttttt ctttttttag cagttcagcc gctccttcga
      481 gtaatgcttt ttcgagctgt aattggtgta cttgtttttg taagtcttct acttccttta
      541 aaagtagctc tttttgtttt tcctgatttt ttaaagtcat acgcaatgga aaatcctttc
      601 caagaaccgt gtttttccat tgatatagag taactctact aattccttca gattcggcta
      661 aatttttcct attatataaa ttgagttaaa ttgtattcgt ttttcctcct ataggcgtat
      721 actaaagaaa acacttgagg agtggggaac atggagacag aacgccaaat ccgtgaattg
      781 gaaaaagcga tgaatacgac agacaaccgc cggttgtacg agcgtttcct ggcagtcaag
      841 ttggttcttg aaggacacac ctggaaagaa gccgggcaca ccatcggccg cgacgagcat
      901 actgtcagcc actatgtgaa aaactactgc cataatggaa tccaaggttt agtctccaaa
      961 aaacaaagcg gacgaccttc acggctgaca gatgaacagg aagcggaact ggtccaaata
     1021 gtcgcctacc acactccgga ggaagtaggt ttcaaatccc gtgccaattg gacattggcg
     1081 attgtgtgtg acctgattct ccgggaatgg agttatggat atacgcaaaa aggcgtggcg
     1141 gacctccttc accggctagg actaagctgg acccgtccga cgtataccct caaaaaagcg
     1201 gattccaaga aacaacaggt atttttggaa gaaaccttcc cgagcctaaa aaaaaaattg
     1261 ctgaatagcg atattgacca cctcctattc gaggacgaaa ccatgatccg ggattaccaa
     1321 gccattggct gcacctggtt tctccgcggc caccagcgaa aaatccccac ttatggacaa
     1381 cacaaagggg taaaattgat aggcaccttg gattatgaag ccggtcggat catttgtaca
     1441 gaagaagggg catacgacgc agcgaccttc ctccgttttc tggagcggac gttgcgggaa
     1501 taccccaccg gaaaaatcgt gatggtattg gataatgccc ttatccacca cgctaagctg
     1561 atccagtctt ttcttgaaga aaacgctagg cgcctggcac tcatcttcct ccctccctac
     1621 agccctcagt tgaacctaat ggaaggcgtt tggaagtggc tcaaggaatc ggtcatcaac
     1681 aatgtttttt tgaccacgtc cagaaaatca aacagtcggt ccgcggtttc ttagccgatg
     1741 taaacgagcg tccccttgag gtgattgacc gactgtgcgt gcggatgtaa agtatttaat
     1801 tcaacctata tagttaattg tagcctatcc aaatcggaat tttaaagcaa agaagccaaa
     1861 tgaaaaatgg ttaacagata ttactgaatt tagaattccc g
//
LOCUS       DACGLH010000173         1892 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.185, whole genome shotgun sequence.
ACCESSION   DACGLH010000173 DACGLH010000000
VERSION     DACGLH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.185"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..1029)
                     /locus_tag="IXT21_002996"
     CDS             complement(<1..1029)
                     /locus_tag="IXT21_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4870333.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1111..1800)
                     /locus_tag="IXT21_002997"
     CDS             complement(1111..1800)
                     /locus_tag="IXT21_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ4870334.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
BASE COUNT          638 a          425 c          328 g          501 t
ORIGIN      
        1 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
       61 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
      121 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
      181 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
      241 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
      301 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
      361 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
      421 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
      481 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
      541 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
      601 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
      661 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
      721 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctac
      781 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
      841 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
      901 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
      961 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtg ttttttcatt
     1021 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
     1081 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
     1141 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
     1201 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
     1261 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
     1321 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
     1381 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
     1441 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
     1501 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
     1561 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
     1621 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
     1681 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
     1741 taccagtatc agaatgattc ccaacgagat caacatcaat cggattattt tctttttcat
     1801 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
     1861 caccgaaatc caacatagga tctcagtgct tt
//
LOCUS       DACGLH010000174         1772 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.186, whole genome shotgun sequence.
ACCESSION   DACGLH010000174 DACGLH010000000
VERSION     DACGLH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.186"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            39..590
                     /locus_tag="IXT21_002998"
     CDS             39..590
                     /locus_tag="IXT21_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ4870335.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            984..1058
                     /locus_tag="IXT21_002999"
                     /pseudo
     CDS             984..1058
                     /locus_tag="IXT21_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003725297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transposition-like protein"
     gene            1058..1738
                     /locus_tag="IXT21_003000"
     CDS             1058..1738
                     /locus_tag="IXT21_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HAQ4870336.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
BASE COUNT          645 a          270 c          353 g          504 t
ORIGIN      
        1 agagcgagca agacaggaac gagagaacaa acatactcat gaaaagacta gaggcttcga
       61 cctcgaactt taataataag ttagatgttt ttgaagaaaa gactaagcat gcaagattaa
      121 actttgaaac aactgcaaag cactatatta aacgacttga tgaagataat ttgaaattag
      181 attttcaaca agctattcaa gatgaattaa gcgatactaa agacgaaata cgtgaagtga
      241 cgaaacaagc gagagaagaa acaaaagagt ataaagaaat cttagaaagt aaaataaaag
      301 accataacaa agtggttgat aaatcgaata cagcgctaaa agtgatgaca aaaggcgtta
      361 caaatatatt ttttgtgcta attatttttg tacttgtaat gcttgtgaca ggtccgatag
      421 gtcatttctt tggtattgaa catttatata gctttattaa tggttttatt gatgaccacg
      481 aaagtgcatg gcgatatttg atgttgatac tttatgcagt accctatgta ttcttcgcta
      541 tcattctgtg gctcatagca ttgtttttta gagtatttaa tgacctttag gtagatttat
      601 atataaaaag ggctaaatac gcaaaatacc cccttaaaat gcaaaataaa tgcaaataag
      661 ggggtaactc cttatgaaaa aacaactaat agtattataa cacgttttta tgccgagaaa
      721 acttattggt tggaatgggc tatgtgttag ctaacttgtt agcgagttgg ttggacttga
      781 attgggatta atcccaagaa agtaccaact caacaacaca taaagccctg taggttccga
      841 ccaataagga aattggaata aagcaacgaa aggagttgag taaaatgagt gagaaaacga
      901 agaaaatcat tcgagtatca ttagcttttt cacaagtagt agtgcaatca ttgatagttt
      961 attatactta ccagcaatat aaatggttct gttgcaaagt tttccaaaaa atctatttta
     1021 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
     1081 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
     1141 tcgtgaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
     1201 tcgttgggtg caagagtaca gcaaagtcct ctattatctt tggaagaaga aaaatagaca
     1261 atccttctat tcatggaaaa tggacgaaac ctatatcaaa attaagggac gttggcatta
     1321 tctttatcgt gcaattgatg cggacggctt aaccttagat atctggttac gaaagaaacg
     1381 ggatacgcaa gcagcctatg ctttcttaaa acgactccat aaacagtttg gtgagccgaa
     1441 agcaattgtg accgataaag caccttctct tggctccgcc tttagaaagt tacagagtgt
     1501 gggtttatat actaagacag agcaccgaac tgtgaagtat cttaacaatt taatagaaca
     1561 agaccatcga cctattaaac gacgaaataa attttatcaa agtctccgta cagcctcttc
     1621 cacgattaag ggcatggaga ccattcgagg aatatataaa aagaaccgaa gaaatggaac
     1681 gctcttcggc ttttcggtgt ctactgaaat caaggtatta atgggaataa cagcctaaga
     1741 tatttggagt tcagagaggg cgcgtttgat tt
//
LOCUS       DACGLH010000175         1715 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.187, whole genome shotgun sequence.
ACCESSION   DACGLH010000175 DACGLH010000000
VERSION     DACGLH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.187"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..1647
                     /locus_tag="IXT21_003001"
     CDS             <1..1647
                     /locus_tag="IXT21_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4870337.1"
                     /translation="IGEAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANF
                     IYSILFTQVVGKSTNRMRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQ
                     ALLQVITNAVLLVGILIMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDE
                     VGKLNGYMDEKISGQRVIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQG
                     MSLVNTAIVIFFGGWLALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLA
                     ITGARRLNEIFDEKDEVKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKG
                     EMVALVGPTGSGKTTIMNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESV
                     LFSGTIKDNITFGKPDASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQK
                     QLISIARTIITNPSLLILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILN
                     ADRIVVLRDGEVIEEGSHHELLAENGFYAELYHNQFVFES"
BASE COUNT          557 a          280 c          378 g          500 t
ORIGIN      
        1 atcggggaag cttttcaaga attagctaat tacgtaggag gcttgatgca aggcgtagac
       61 gataaaagca aatttgtttc tgtaatatgg aaactcttgc tgttttatgt actagctagt
      121 gcagccaact ttatctatag tattttgttc acacaagtag tagggaagtc aacgaaccga
      181 atgcgtatcg gtctattcaa caaattggaa aaattgacga tccgtttctt tgattctcat
      241 caagacggtg aaattttaag tcgttttacc agtgacttag ataatattca aaatagtttg
      301 aaccaagctt tgcttcaagt catcacgaat gctgttttac ttgtcgggat cttgatcatg
      361 atgttccgcc aaaatgtaga gttagcttgg gctacaattg cttccactcc agtagccgtt
      421 ttgatagctg tcttgattat ccgcaaagca cggaaatatg tggatcttca gcaagatgaa
      481 gtggggaaac taaatggtta tatggatgaa aaaatcagcg gacaacgtgt catcatcaca
      541 aatggcttac aagaagaaac gattgaagga ttcttagagc ataatcaaag tgttcgcgaa
      601 gcaaccttca aaggacaagc ttattctggt ctactatttc cgatgatgca aggaatgtca
      661 ctggtcaata cagcgatagt tatctttttc ggcggctggt tagcattaaa tggtgatttg
      721 gaacgttcgg tggcattagg cttagttgtg acattcgtcc agtattcaca acaatattac
      781 caaccactga tgcagatttc atctggatac agcatgatcc aattagcgat tacaggggca
      841 agacgattga atgaaatatt tgatgaaaaa gatgaagtca aaccagaaaa cggtaaagca
      901 tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag attttggtta ttctccagaa
      961 aaactagtct tgaaagatgt ctcgatcaat gtggacaaag gagaaatggt tgctcttgtt
     1021 ggtccaacag gatcggggaa aacgacgatc atgaatctgc taaaccgttt ttacgatgtc
     1081 aatgatggtt cagtagtcat cgatggaata gatatccgag attatgatct tgatgcatta
     1141 cgtagacatg taggaatcgt tttgcaagaa tctgtattgt tctccggaac gatcaaagac
     1201 aatatcacat tcggcaaacc ggacgcttca gatgaagaag tcatcaatgc agcaaaacaa
     1261 gcgaatattc atgacttcat catgacgtta gaaaaaggtt atgatacaga gatatccgaa
     1321 gaaaataact tattcagtac aggtcaaaag cagttgatca gtattgctcg aacgatcatc
     1381 acaaatccat ctttattgat tttggatgaa gcaacaagta atgtagacac ggtaacagaa
     1441 gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa gaaccagctt tgtgattgct
     1501 caccggctga aaacaatctt gaatgcagat agaatcgttg tcttgcgtga tggggaagtc
     1561 attgaagaag gcagtcatca tgagttatta gcagaaaatg gattttatgc ggaactttat
     1621 cataatcaat ttgtatttga aagttaaatt aaaagaacct cgttataacg gggttctttt
     1681 tatatatttt atgttgtttg tttttataag gggca
//
LOCUS       DACGLH010000176         1712 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.188, whole genome shotgun sequence.
ACCESSION   DACGLH010000176 DACGLH010000000
VERSION     DACGLH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.188"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..161)
                     /locus_tag="IXT21_003002"
     CDS             complement(<1..161)
                     /locus_tag="IXT21_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4870338.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
     gene            364..1296
                     /locus_tag="IXT21_003003"
     CDS             364..1296
                     /locus_tag="IXT21_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ4870339.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1368..>1712
                     /locus_tag="IXT21_003004"
     CDS             1368..>1712
                     /locus_tag="IXT21_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870340.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          709 a          208 c          276 g          519 t
ORIGIN      
        1 tattctaagg ctgattttcc ttgcttgaag aacagatata ctttatggat cgtttgtctt
       61 gaacgattca agcaatgggc agttttagca acagattgat tcattttgaa ataagactct
      121 attattacaa gttcatccgt tgtaagatgg gtataggtca tttgtactca ctccttataa
      181 ttctttggtc ggaactattt tgagtgtagc acaaatgact tttttagttg tctagcttaa
      241 ttttacaatc ggcgtgatga aataaatcaa aagtatgatg aaacattaaa aaatgtctat
      301 tcttcactta tcgtttcaaa tatagaagaa acttcatctt ctgaaataaa aaataagctt
      361 tctttgttaa aattaaaaga aaaggaatat attattgacg atgctgtttc tgtccctctt
      421 attttaaata aacgagagaa aaataattta aagaaaaaaa tgctcattcc atttgattca
      481 gaagtgaaag gattactcaa taaactaact atttctaata tagactcgac tagggaaaaa
      541 atcataaaaa tatttgataa aattaataat ttattcaaga atgacaatgc tgaattaagg
      601 aagggtcttt tagagtttaa acttcaagaa ctagaacttc attactctta tttagtgaaa
      661 attaccgaag aaaaagaaca acaaaaagca ataaaagaac agatgataga agaggaaaaa
      721 gttcgtagag aaattgatcg tgaaaagaaa cgtattgata aagaacaacg tcaattcaat
      781 tctgaaattt ccaaattaat cgcatatatg caaaaagcta atgcagatgt ggaaaaagaa
      841 ttatatgcaa ataaaattca agaactggaa gaaaaattaa aagaattaga agttatacgt
      901 gaaaatgtat tacaaagaga attaaatact agagccggtt atgtgtatgt aattagtaat
      961 attgggtctt ttggtgaaga catatataaa attggtatga ctagaagatt agaacctatg
     1021 gatagaataa aagaattgag tagtgcatcg gttccatttg aatttgacgt tcatgcgatg
     1081 attttttcag aggacgctcc gtctttggaa actaaattgc ataatcattt tagaaaacaa
     1141 gaagttaata aaattaatca aagaaaagaa tttttcaaag tttcactaga cgaaatagag
     1201 aaagttgttt tagaaaatta taatggaaca gtaacattta caaaattagc taaagctgaa
     1261 caatatagac gatctttaga attgtcaaaa gattaaatat ataaaaagcc ttcgggcttt
     1321 ttcttttaac aattaatagg aacgtatatt cgaaaggaat agaaataatg tggttagaaa
     1381 aaataaatga caaaaaattt aagtacaccg aacgttatac ggatccctta actgaaaaaa
     1441 agagaaaagt gtccgttact ttatctagca attcacgcca agcttggaat caagctaact
     1501 tacttttgaa tgaaaaaatc gcagaaaaaa taaaacgaaa tgaagaatta ccactaactt
     1561 ttggagaatt aaaaactaaa tgggatgaaa aatacaagcc aactgtaaaa gaaagttcct
     1621 atcgaacaac tcaggtttat ctatcattaa tttcaaaata tataaaagat gatgtacttg
     1681 taaaaaacgt taatagtaat cttatacaag at
//
LOCUS       DACGLH010000177         1584 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.189, whole genome shotgun sequence.
ACCESSION   DACGLH010000177 DACGLH010000000
VERSION     DACGLH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.189"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            292..1479
                     /locus_tag="IXT21_003005"
     CDS             292..1479
                     /locus_tag="IXT21_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ4870341.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          316 g          505 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta cttg
//
LOCUS       DACGLH010000178         1578 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.190, whole genome shotgun sequence.
ACCESSION   DACGLH010000178 DACGLH010000000
VERSION     DACGLH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.190"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            378..>1578
                     /locus_tag="IXT21_003006"
     CDS             378..>1578
                     /locus_tag="IXT21_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ4870342.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTL"
BASE COUNT          516 a          300 c          283 g          479 t
ORIGIN      
        1 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
       61 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
      121 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
      181 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
      241 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
      301 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
      361 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
      421 atgtttgttt tttaacagac gacaatcaat tgtctatctt atctgaaatc tctgttgcca
      481 atgacatcga aggtgcttca ttcatacgag aaacaattct tgaattcaat aacagctacc
      541 actttgatca aattgtgatt ggcatggagt caacgtccat gtacagcttc catccttcca
      601 tgttttttca tgaagatgag aaactaaaga agctcaacac acttgtaacg attgaaaatc
      661 cttttcgggt gaaacaattt agtcgcatgt ttgatgagaa taaaactgat cgaaacgatg
      721 cattaagaat cgctgatttc ttgcgtattc aacgattcac aacttcccct atcaaagaag
      781 agaaatacat ggccttacag cgtttaacac gtactcgcta tcaacttatt aagcaattga
      841 ttcgtacaaa gcaacatttt cttgaaaatc taacgtacaa atgtaatacg cttgctcgag
      901 aaatgcgaga cgaatcgacg agtcttttta gtgcaaccct catttcactc atgacggaag
      961 atttcacatt ggatgaactt gctgagcttc ctttagaagc tttctgtgat ttacttcaag
     1021 aaaaaggaaa aggccgtttc aagcaaccag aaaaaatcgc taaagcaatt caacgtgcaa
     1081 ttaccatgag ttaccgccta ggtgctcttg cacaagaatc aattaatgtt gttttaagtg
     1141 ttctcgtacg agaaatacga gcacttgaaa aaaatatcaa agaattagat aaagccatcg
     1201 aacaagtaat tgttgtcatt ccagaatatc aatgcttaac cagtattcca ggcgttggta
     1261 aagtctatgc tgccggttta atcgctgaaa ttggtcaaat cgaaagattt gaagatcaga
     1321 caaaattggc aaaatacgct ggattaagtt ggaaagtgaa ccaatctgga aactatcagt
     1381 cgcaaaatac acctctgaca aaacaaggaa atcgatattt tagatactac ttagttgaag
     1441 ctgccaactc tgtaaaaaat tatcttcctg aatacaaagc tttttatcaa agtaaatata
     1501 aggaggttcc aaagcatcaa cacaaacgtg cactcgtcct aaccgcaaga aaatttacgc
     1561 gtctggtgga tacgctac
//
LOCUS       DACGLH010000179         1573 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.191, whole genome shotgun sequence.
ACCESSION   DACGLH010000179 DACGLH010000000
VERSION     DACGLH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.191"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DACGLH010000180         1508 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.192, whole genome shotgun sequence.
ACCESSION   DACGLH010000180 DACGLH010000000
VERSION     DACGLH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.192"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..>1508)
                     /locus_tag="IXT21_003007"
     CDS             complement(<1..>1508)
                     /locus_tag="IXT21_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870343.1"
                     /translation="GGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYK
                     HCYVEYRKDNQILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEI
                     KGRVDSLNQQITDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPN
                     LMPYITEPWVGPLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRY
                     LITTLRPSTTYTLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERD
                     KMVTHTFTGITKDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWL
                     GKVPLGENIADPTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYI
                     AAGRVKVGDMKPTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLK
                     LEKGNTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEIT"
BASE COUNT          424 a          299 c          255 g          530 t
ORIGIN      
        1 gtaatttcag aaattttaat atctgtggcc aaattatctt cgacatattc tggtgctaga
       61 tcccaaacat aatcttttgg attgtttgaa tcacgcatac cagtaccacg atatttatac
      121 tcactaatat tcggggttcg agtattgcct ttttctagtt tgagccactc aatttgaaca
      181 gctccttttg tagctgacgg gtattggtat atttccagat aattagttac cccactatct
      241 atatgttgct tagttacagt aaaagttaac tcccatgtat tagctaaccc ctcagtaggc
      301 ttcatgtctc ctactttaac gcggccagca gcaatataca cggcaaacga ttgaactgtt
      361 agtttagttg ctttcattga tatggtatat gtttggtcag cgatatagtt ttctgtattg
      421 cgtctaccat aaaccatata ttcactagtt ttgtgtggaa agacaactgt agggtcagca
      481 atattctcgc ctaaaggaac tttacctaac cagtaagggc catctagtaa gttagggtag
      541 tgtgctttta acttaacgtc atagctgaca ctaaccgttc ctttatattc actattaagt
      601 gagaatattt caacataaca gtttgttatg ctagttacat tatcttttgt tataccagta
      661 aatgtatgag tgaccatttt atcgcgttct acatttgctg gtattaatgc attaatgggc
      721 agttctatac caccttgttc atttaaatat ctaagcctaa cccctatagt attggtctct
      781 ccagtccaat ctgaaccgac actcatagta acgcttaatg tgtatgtcgt agatggtcgt
      841 aaagtggtta taagatacct attattagct tccgctgtac acggaatacc taagccaagc
      901 gataatatgt cgccggaatt agctgttcta gttttagtat gagttataac ccttttaaca
      961 ctatctttaa ctgtatgtcc attacctaac aaaggtccaa cccaaggttc tgtaatatat
     1021 ggcattaaat tcgggttctc cgaataatca tagtccccga agtcgatgct gttactgtac
     1081 atcacttgta agttacctaa cttagaaatt tcttctttca gagcatctaa cttgtcttgt
     1141 agcgttttag cttgaccagt taaatcagta atctgttgat ttaagctatc cactctacct
     1201 ttgatttcag ccataaaagc atcaaaagtt tcgttgtact ttcgaatcaa ctcttctaat
     1261 tgcgaaacat attcatcggc ttggccttgc gaaatgtcag acactcctag tgagaaaaaa
     1321 atgatatctt gcgttgttaa aatttgattg tcttttctat attctacgta gcagtgttta
     1381 taatatcctg cttcactcat aaatgtgcca tcaagagaaa acgtgacttc ttcactagtt
     1441 acactagttg caacattatc tacgtaacgg ttagatggtg ttgttccttt taaagtaaat
     1501 gttccgcc
//
LOCUS       DACGLH010000181         1495 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.193, whole genome shotgun sequence.
ACCESSION   DACGLH010000181 DACGLH010000000
VERSION     DACGLH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.193"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(10..369)
                     /locus_tag="IXT21_003008"
     CDS             complement(10..369)
                     /locus_tag="IXT21_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ4870344.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(366..1217)
                     /locus_tag="IXT21_003009"
     CDS             complement(366..1217)
                     /locus_tag="IXT21_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4870345.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1234..>1495)
                     /locus_tag="IXT21_003010"
     CDS             complement(1234..>1495)
                     /locus_tag="IXT21_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAQ4870346.1"
                     /translation="KVDIEKWADTIRLMEERDKASIEAIEYVINWLPTNEFWFGNIRS
                     AKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI"
BASE COUNT          366 a          320 c          226 g          583 t
ORIGIN      
        1 accacctcgt taaaaacgct cttccttgaa cgtattccga tattttttag ctaatatcaa
       61 cggcgcttga tattgatgac agaacaactt tgccttgatc ttaaagtctt ttgtctgcat
      121 tcctttgaca tctacgactt tgacaagttt gccgttttta taaaatgtga agtcgggaat
      181 atactcgatc ttgcgatact tctttccgtc tagttcaaat ttcggcatca gctcaaatct
      241 ttcctgaagt tttactttcc agccatttgc ttcagcttgc cacaaggcta gatcgtaata
      301 ctctgcttct gcgatagaat caaacttgat acctcgatga acagtttttt tattacggta
      361 tttattcatg cgatactacc tttcactggt tttatgcgct tgtctgctgt ttgttggaat
      421 ttcagcgcat aaccttctga attcttaaat atcctagaaa caattctttc accgtaggct
      481 tctcttagtt caggaccaga taagtttgtt gtgatgatcg ttgccttgtt ctgtctggct
      541 tctaagagcg tgtttaacgt gttgtttgta aactgcctac tatttgatac cccactacct
      601 aattcagctc caatatcgtc aaaaaccacc aaatcagttg ttttaatatc ggctataagc
      661 gatccttcaa tttcttttct cagttcagta ttgttataag aaaactttat ttgctctaat
      721 aattcttgat agcttataaa aagtattttc ttgtcataat ttgagcgctc aagtatttcc
      781 caagctgtgg ccattgataa gtggcttttt ccgcttcctg atttccctga tagaatgaaa
      841 tgtgcaggat ggttcagcag aacctcattc gtatagcttt ttgctttttc taaagcgatt
      901 tttgtttctt gatccactac gtgataattt tccattttgc atttaaacaa agttttatct
      961 gttaataccg aaccattttg aaaaaaactc aacgctcgtg cttttaagct atcgttatat
     1021 atccgttcgg tctgtatatc ctctttcaca cgtaacgctt tataaccaca actcatgcat
     1081 gttggtttac aacgttctga accatcctta tttttagctc gccaactata caaaggttcg
     1141 ctacattctg gacattttcc gctttgcact aatactcttc ttattagttt ctccatagca
     1201 tttgctaggc tttccatgtg atgcatctcc tttttaaatt ggcaagtcgt catattcact
     1261 aggattgctg tactgtagtt tttgactttg ctttttatga ttcttcttgt ctgctttgat
     1321 ttcgaatttg agcttctcaa atttttctct caatttctta gcacttctaa tatttccaaa
     1381 ccaaaattca tttgtaggta gccaattgat cacatactca atcgcttcta tagatgcttt
     1441 atctctttct tccatcaacc tgattgtgtc tgcccatttt tcgatatcta ctttg
//
LOCUS       DACGLH010000182         1478 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.194, whole genome shotgun sequence.
ACCESSION   DACGLH010000182 DACGLH010000000
VERSION     DACGLH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.194"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            69..803
                     /locus_tag="IXT21_003011"
     CDS             69..803
                     /locus_tag="IXT21_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ4870347.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          554 a          236 c          200 g          488 t
ORIGIN      
        1 aaaaaataag tggttataat ggattgttaa tataaacatt cattataacc tcataggagt
       61 gggttataat gaacaaaaaa aatataaaag atagtcaaaa ctttattact tcaaagcatc
      121 atataaatga aattttgaga aatgtacatt taaatacaaa tgataatatt attgagattg
      181 gttcagggaa aggtcatttc acgtttgaat tagctaaaag gtgtaattat gtaaccgcca
      241 ttgaaataga tcctaaatta tgtaggataa ctaaaaacaa acttattgaa tatgagaact
      301 tccaggttat caataaagat attttacaat ttaagtttcc taaaaataag tcatataaga
      361 tatatggaaa tataccctac aatataagta cagatataat tcgaaaaatt gtttttgaaa
      421 gcacagctac agaaagttat ttaatagtgg aatatggatt tgctaaaagg ttgctaaata
      481 caaatcgttc actagcacta tttttaatga cagaagttga tatatccata ttaagtaaaa
      541 tccctagaga atactttcat ccaaaaccta gagttaatag ctcgttaatt gtattaaaaa
      601 gacacccttc aaaaatatca ctcaaagata gaaaacaata tgaaaatttt gttatgaaat
      661 gggttaacaa agaatacata aaactatttt ccaaaaacca attttatcaa gccttaaaat
      721 atgcaagaat tgacgattta aacaatatta gctttgaaca attcttgtct cttttcaata
      781 gctataaatt atttaataga taagttaagg ggtgcataaa ctgcatccct taacttatct
      841 tattttgctt aaacaatctt ggcccgtaaa ctgcataact catatgtaaa tcgctccttt
      901 ttaggtggca caaatgtgag gcattttcac tatttccggc aattacttcc atgtaaagta
      961 taacccacta tactttgcaa gcaaagtgtg ggctcgccga ggggtcttgc agacggctct
     1021 ttcggctctt ttgagccgag tatcagcgca cccttatggt gcaattagct gatacctgtt
     1081 tgacgatttg ctgccctacc ttttcggtag gtatgcaaac ctcaaaccaa aataaaaacc
     1141 cccttcgact tcgtcagggg gtttttattt atataaatct ccgtaaataa tcgcgattta
     1201 ataacatctt caattttatt tttttgcgta ttttagaaat gttttcacta aaaaatatat
     1261 tatacaaaaa atacagtaat cgattataat tctttgccag ttaaatatca tatcgttatt
     1321 aaaatatcgg aaaaaaatat caacaacaat aaatgcaaaa gaagaccata aaaattgttt
     1381 attactcata catactctcc atttctatca tttttaaatt taccagtcat ctctcaaaaa
     1441 tctcctagct atttttcttt tttaaccaca taccgtca
//
LOCUS       DACGLH010000183         1458 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.195, whole genome shotgun sequence.
ACCESSION   DACGLH010000183 DACGLH010000000
VERSION     DACGLH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.195"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            336..1439
                     /locus_tag="IXT21_003012"
     CDS             336..1439
                     /locus_tag="IXT21_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ4870348.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDWGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          473 a          237 c          328 g          420 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagattggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaa
//
LOCUS       DACGLH010000184         1440 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.196, whole genome shotgun sequence.
ACCESSION   DACGLH010000184 DACGLH010000000
VERSION     DACGLH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.196"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            105..1406
                     /locus_tag="IXT21_003013"
     CDS             105..1406
                     /locus_tag="IXT21_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4870349.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DACGLH010000185         1418 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.197, whole genome shotgun sequence.
ACCESSION   DACGLH010000185 DACGLH010000000
VERSION     DACGLH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.197"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(150..1337)
                     /locus_tag="IXT21_003014"
     CDS             complement(150..1337)
                     /locus_tag="IXT21_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ4870350.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DACGLH010000186         1370 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.198, whole genome shotgun sequence.
ACCESSION   DACGLH010000186 DACGLH010000000
VERSION     DACGLH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.198"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..843
                     /locus_tag="IXT21_003015"
     CDS             <1..843
                     /locus_tag="IXT21_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4870351.1"
                     /translation="TKFVCAIGDEEMTIKERVSFPTTTPEETMPLVIDFFKQYQADLA
                     GIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPISWTTDVNAAAY
                     GEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGS
                     CPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVI
                     IFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREA
                     VLHS"
     gene            complement(1077..1370)
                     /locus_tag="IXT21_003016"
                     /pseudo
     CDS             complement(1077..1370)
                     /locus_tag="IXT21_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          402 a          265 c          304 g          399 t
ORIGIN      
        1 acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt
       61 ccgacaacga cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa
      121 gcagatttag cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc
      181 gccacttatg ggtacattac ctcgaccccg aaattggcat ggcaaaattt tgatttcatc
      241 ggtaccatga aaaaagaatt tccgatcccg atttcttgga caacagatgt caatgcggct
      301 gcctatggtg agtatgtctt cggcagtggc aaaggattga gcagtgtcgt ttactatacg
      361 atcggtacgg gtgttggcgg cggtgcctta caagaagggc gctttatcga agggtttagt
      421 catccagaaa tggggcatat gctcgtcgtc cctcatccaa aagatgactt tgctggcagc
      481 tgtccatttc atggcaactg tttagaaggg atggccgctg gacccgccat cgaaaaacgc
      541 ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt attgggaaat cgaagcctcc
      601 tatattgctc aatgtgccta caatacaacc ttgatgtttt ctccagatgt gatcattttt
      661 ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa
      721 ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat atatcgtgac cccagcatta
      781 gaagacaacg ctggaacgct cggttgtttg gcattggctc gagaagcggt cttacattca
      841 taaacgaaaa aaggctcttc gacatttagt gttggtagag aaagatacaa ctgaaaattt
      901 agtcataacc agttaggcag agtgcttaac tggtttttgt tttacttgga agatcaagcc
      961 ttgaatgaga taaattcttg ctcgaaagtt atggaaattt cgatagccgt aggctacttg
     1021 tattgtgcaa tagtttttta caaaaaaaag gggtgcggat atttttttgg acctttttaa
     1081 caaatatatc tttgcttctt ccgatattga agagggctta gccctcctaa agataactta
     1141 attcgttgat tattatacca atgaatataa ttatccaatt tgtttataaa ctgattgatc
     1201 gttgtattct tccagtcacg ttgataaaac atctcgtttt ttaatcgtcc aaagaaacct
     1261 tcgcacgctg aattatctgg tgaataaccc tttttagaca tagagtgaag caagttgtct
     1321 ttgttccaat agtccaacta acaatagcac catcgtaaca atcgacaagt
//
LOCUS       DACGLH010000187         1354 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.199, whole genome shotgun sequence.
ACCESSION   DACGLH010000187 DACGLH010000000
VERSION     DACGLH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.199"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            82..>1354
                     /locus_tag="IXT21_003017"
     CDS             82..>1354
                     /locus_tag="IXT21_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ4870352.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLI"
BASE COUNT          460 a          261 c          257 g          376 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atca
//
LOCUS       DACGLH010000188         1323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.200, whole genome shotgun sequence.
ACCESSION   DACGLH010000188 DACGLH010000000
VERSION     DACGLH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.200"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            54..1216
                     /locus_tag="IXT21_003018"
     CDS             join(54..330,330..1216)
                     /locus_tag="IXT21_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ4870353.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          478 a          234 c          264 g          347 t
ORIGIN      
        1 acagaaaaat taaggtgtgt agactagaag aaaacatacc aggaggaatt tttatgtcta
       61 agagaacacg aagaactttt tcacaagaat tcaagcaaca aatcgtcaat ctttacttag
      121 ctggaaagcc acgtgtagaa atcattcgag aatatgaact aacggcttca gcatttgaca
      181 aatgggtaaa gcaatctaaa acgagtggtt cattcaaaga aaaagataat cttacgcctg
      241 aacaaaaaga attgttagaa ctacgtaaaa gaaaccagca attagaaatg gaaaatgata
      301 ttttaaagca agcagcgctg atattcggac gaagagacaa gtaatcgatg cgaataagca
      361 tctttaccct atatcagcga tgtgcagaat attaggtcta tcacgtcagt cctattatta
      421 tcaatcaaaa ccaaagaaag acgaatcaga acttgaagaa gtagtcgctg aagaatttat
      481 ccgcagccga aaggcctacg gctcaagaaa aataaaaaaa gccttatcaa aacgaggcat
      541 tcagatcagc cgacgaaaaa ttagtagaat catgaaaaat agaggattaa aatcgagcta
      601 tactgttgct tattttaaag tacatcattc tacttgcaat gaagccaaaa cgacaaacgt
      661 attgaatcgt aaattcttaa gagacaaccc attagaagcg atcgtaacag acttgactta
      721 tgtacgagtc gggaaaaaat ggaattatgt ctgtttcatt ttggatctgt tcaatcgaga
      781 aattctcggc tattcttgtg gagaacataa agatgccgtt ctagtaaaaa aagcatttag
      841 ccgtatcaaa caacctctga cagaggttga gatttttcat actgatcgtg gaaaagagtt
      901 tgataaccaa gctattgatg aattattaac aacttttgac atcaatcgat cattgagtca
      961 taaaggctgt ccttttgata atgccgtagc tgaatcaact tataagtcgt tgaaagtaga
     1021 atttgtctat caatacacat ttgaaacctt acaacaattg gatttggagt tatttgacta
     1081 tgtcaattgg tggaaccacc ttcggttgca cggtacactt ggctacgaga caccggttgg
     1141 ttaccgtaac cagagattgg cgcagcgaat ccttgataat gagctcggat gtgctaacgc
     1201 tagcgaggca gtctaacttt aactgttagc tcctgccgaa gatcgtcaca tccgaggagg
     1261 ctcattgtca aggacaatcg gaatagcata cggaaagaag ttctgcacct tataaaattt
     1321 gtc
//
LOCUS       DACGLH010000189         1322 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.201, whole genome shotgun sequence.
ACCESSION   DACGLH010000189 DACGLH010000000
VERSION     DACGLH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.201"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            96..>1322
                     /locus_tag="IXT21_003019"
     CDS             96..>1322
                     /locus_tag="IXT21_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4870354.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYG"
BASE COUNT          415 a          263 c          254 g          390 t
ORIGIN      
        1 cttcaccaac atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga
       61 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
      121 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      181 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      241 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      301 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      361 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      421 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      481 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      541 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      601 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      661 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      721 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
      781 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
      841 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
      901 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
      961 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
     1021 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
     1081 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
     1141 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
     1201 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
     1261 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
     1321 gc
//
LOCUS       DACGLH010000190         1274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.202, whole genome shotgun sequence.
ACCESSION   DACGLH010000190 DACGLH010000000
VERSION     DACGLH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.202"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            33..530
                     /locus_tag="IXT21_003020"
     CDS             33..530
                     /locus_tag="IXT21_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4870355.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(513..1250)
                     /locus_tag="IXT21_003021"
     CDS             complement(513..1250)
                     /locus_tag="IXT21_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870356.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKG
                     KRIMVPLPVQKNVLSIKSEDVFLDFFF"
BASE COUNT          443 a          216 c          224 g          391 t
ORIGIN      
        1 ttatagtata ctatgtcaaa aggaggagtt agatgaacaa tcaaatcaca ctagcgtatt
       61 tagattatga tgatctagat gctttacaag ttgtaagtgt tgtcagtttt tatgaatcgt
      121 ttatcgatgg cgcagatcca cttgatatgc aaggatattt acagacacaa ttgacaactg
      181 aaatattggc agtagaattg gcacagtcaa ccagtaaatt tattggtatt aaggatcatc
      241 aaatactcat tggttatatg aaagtcaatg atgaaaaaga tgctatagag attcaacggc
      301 tctatctgct caaagattat caaaacaaag ggttagggca acggttactc gatgaagcga
      361 atcgttatgc taaaacgaaa caaaaacgtt atttacgttt gactgtttat gaaaaaaatc
      421 atgcaggtat tcgtttttat gaacgtcatg gatttaaaaa aatcgggatc aaacattttc
      481 ctttaggaaa acaagatcgt atttgtccga ttttagaaaa agaaatctaa aaaaacgtcc
      541 tcacttttga tgctgaggac gtttttttgg actggtaatg gaaccataat tcgctttcct
      601 ttttgataaa tcgctaattc cgtcacacct tttgctttta acagagcaaa aagatcatcg
      661 aaatggaaac ggaaatgttc aaggtaatgc ccatctgatc caacactaaa caaggtacca
      721 ccacgtgaaa gatataaatc gatgacatat tcatataacg ctaaattgtc atacaagtaa
      781 gcgctcttag catttaattc gaatgccaat ttatttttaa ttacttgatc tagaatcggt
      841 aaaaactgcg cttcatattg tttaaattca gcaacggata gaccaaatac ccgaaaacca
      901 tagtcaaaat gagcaaagac atctgcttct ataacaacta gtgcttctga aagttgtgca
      961 aaatactgag gaatcacaat agcaggatcc atatctttga cttcgtcatc taaataatca
     1021 aattgaccat tatgatgcac actgagtaat tttaaatcgt aatctttatc ggctaaatag
     1081 tcgatgatcc gatccttttc agaagcgatg taaccgattt cgatgccttt taaaacacga
     1141 ttgccaaaac gcttatttaa gctatcgatt gtttgactgt acatttcata atcggggata
     1201 tcatcgactt tagtaacagg attggctaag tcaaaatgtt cagttgtcac gacgtaacca
     1261 tcgtagtttg ttaa
//
LOCUS       DACGLH010000191         1272 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.203, whole genome shotgun sequence.
ACCESSION   DACGLH010000191 DACGLH010000000
VERSION     DACGLH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.203"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..>1272
                     /locus_tag="IXT21_003022"
     rRNA            <1..>1272
                     /locus_tag="IXT21_003022"
                     /product="23S ribosomal RNA"
BASE COUNT          338 a          285 c          384 g          265 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg ta
//
LOCUS       DACGLH010000192         1270 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.204, whole genome shotgun sequence.
ACCESSION   DACGLH010000192 DACGLH010000000
VERSION     DACGLH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.204"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            69..1241
                     /locus_tag="IXT21_003023"
     CDS             69..1241
                     /locus_tag="IXT21_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ4870357.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          469 a          235 c          226 g          340 t
ORIGIN      
        1 ggccatataa cagtcctttt acggtacaat gtttttaacg acaaaaacat acccaggagg
       61 acttttacat gacccaagta cattttacac tgaaaagcga agagattcaa agcattattg
      121 aatattctgt aaaggatgac gtttctaaaa atattttaac aacggtattt aatcaactaa
      181 tggaaaatca acgaacagaa tatattcaag caaaagaata tgaacgaaca gaaaaccgac
      241 aaagtcaacg aaatggctat tatgagcgca gctttacgac acgtgtaggc acgctagaat
      301 taaaagtacc cagaacacgt gatggccatt tttcacccac agtgtttgaa cgttatcaac
      361 gaaacgaaaa agccctcatg gcttcaatgt tggaaatgta tgtatcaggc gtttcaactc
      421 gtaaagtatc aaaaattgtg gaagaacttt gtggtaaatc cgtctctaag tccttcgttt
      481 ctagcttaac agaacagcta gaacctatgg ttaacgagtg gcagaatcgt ttattatcag
      541 aaaaaaatta tccttactta atgaccgatg tactctatat aaaagtacga gaagaaaatc
      601 gagtactctc aaaaagctgt catatagcga ttggaataac caaagatggc gaccgtgaaa
      661 ttatcggctt catgattcaa agtggcgaaa gcgaagagac ctggacaaca ttttttgaat
      721 acctaaaaga acgcggttta caaggtacgg aactcgttat ttctgatgcg cacaaaggat
      781 tagtctctgc cattagaaaa tccttcacca acgtaagttg gcaaagatgc caagttcact
      841 tcctaagaaa tatctttacc accattccta aaaaaaattc aaaatctttc agagaagctg
      901 ttaaaggaat ttttaagttc acagatatta acttagcgcg tgaggctaaa aatcgattga
      961 ttcatgatta tatcgatcaa ccaaaatatt caaaagcttg cgcatcattg gatgatggat
     1021 tcgaagacgc ctttcaatat accgtacaag gaaattccca caatcgacta aagagtacca
     1081 atctaattga acgactgaat caagaagtac gcagaagaga aaagattatt cgcatcttcc
     1141 ccaatcaaac atcagccaat cgcttaattg gagccgttct tatggaccta catgatgaat
     1201 ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa aaacattgta
     1261 tagcatttta
//
LOCUS       DACGLH010000193         1270 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.205, whole genome shotgun sequence.
ACCESSION   DACGLH010000193 DACGLH010000000
VERSION     DACGLH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.205"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(31..1203)
                     /locus_tag="IXT21_003024"
     CDS             complement(31..1203)
                     /locus_tag="IXT21_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAQ4870358.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          344 a          265 c          245 g          416 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc
//
LOCUS       DACGLH010000194         1245 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.206, whole genome shotgun sequence.
ACCESSION   DACGLH010000194 DACGLH010000000
VERSION     DACGLH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.206"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            199..741
                     /locus_tag="IXT21_003025"
     CDS             199..741
                     /locus_tag="IXT21_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870359.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            831..>1245
                     /locus_tag="IXT21_003026"
     CDS             831..>1245
                     /locus_tag="IXT21_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ4870360.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          513 a          141 c          238 g          353 t
ORIGIN      
        1 ttgatgaagc agatcccaaa gggctgcttc atcaattttt tcgtaagtgt ccgtacactt
       61 actgtgcttg ctcatatatt aatcgatcat atactaatcg tgtaagaaca actttaagca
      121 taatacttat attgttatac catagtttgg aaaaataaaa aattatattt catcaacgtt
      181 tcataaaagg tgtgtagaat gttattgaaa gtagctataa aatgctattg tgtaaaaaat
      241 aaaggaggaa taatttatga aaaaatagta ccaagaggag catgcctatt acttgcatta
      301 tgtttgagct taagcgcagt tgggtgtcaa aaaagtaatg aaaacaataa aataaaagaa
      361 ggacaatcca taagttctga agaggaaggc atgtcaatta atgcaaatgg caaaaatgaa
      421 acttttaagc catcagatta tacattggag gcaaagaaag aatatgttta tgaatattta
      481 gggcttaaat ttaaactctc agacaaattt agaaattata tagctgataa aaagatagcg
      541 atgttagatg atcaaagtcc tatagataaa gaactaaagt atgctatttt gacatttgag
      601 aaaatgacag aagaacaaaa gaatgcagtt atagaaaaga tgggcgatgg atataaaaat
      661 tggcaaaatg agcttgaaag aattgggact attggtatat ttgaaaaaaa tacatcagaa
      721 gaaaaaaatc taaaattata aaatgtgata ctcatactaa aataggagtt tcaagtgatg
      781 gaaaatacga ttgctattta agcacaaata gcggagctga aagtaatctt ctggatgaat
      841 taaaaagaac tgagattcaa attatagata aaaaagaacg tcctaaaaat ggttttgtgc
      901 tatcagaaaa aactgattta gaaaatacag aagcatttaa caaagaatcg gttaaagatt
      961 tacgtaaact accaacaaag gacataaatg gcaaggattt tacaagtaaa gattttgaga
     1021 agtatgatct tactatggta aatgtatttg caacttggtg tacagcttgt gtaaaggaaa
     1081 ttcctgattt ggttgaggtt cagaatgaaa tgaaaagcaa gggtgtaaat atcgttggag
     1141 ttgttacaga tcctgttgat gataacggtg aaaacaagga agcaattgaa aagtcaaagt
     1201 taatacatga aaagacaaag gcttcatatc ctttcttaat gccag
//
LOCUS       DACGLH010000195         1220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.207, whole genome shotgun sequence.
ACCESSION   DACGLH010000195 DACGLH010000000
VERSION     DACGLH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.207"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            141..398
                     /locus_tag="IXT21_003027"
     CDS             141..398
                     /locus_tag="IXT21_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870361.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IXT21_003028"
     CDS             complement(630..1043)
                     /locus_tag="IXT21_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ4870362.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          464 a          154 c          152 g          450 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag catttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga
//
LOCUS       DACGLH010000196         1101 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.208, whole genome shotgun sequence.
ACCESSION   DACGLH010000196 DACGLH010000000
VERSION     DACGLH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.208"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            300..833
                     /locus_tag="IXT21_003029"
     CDS             300..833
                     /locus_tag="IXT21_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ4870363.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          353 a          205 c          203 g          340 t
ORIGIN      
        1 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag aagttgtgaa
       61 aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa atggttcctc acaactttgc
      121 ataaatttga cttaataccg agaagtcatg atccccatct aactccaaat cacaaggaat
      181 gagttcaaaa actagctttt ctcaacaaat attacataaa aaattctatg ttttttgtta
      241 aatttatttg cttttttcaa aagtatgtta cagtaatttc ccaaggaggt tttacagaaa
      301 tgaattttgt tgccatggat tttgaaactg ccaatcatca gccttatagt gcttgttctc
      361 ttgcgctagt catggtaaaa aatagccaga tcgttgatga attctataca ttgatacagc
      421 ctgagactcc ttttttctgg cgaaatgtcc aaattcacgg aatccatcaa gaagacgtac
      481 gcaatgcgcc gaaattccca gaagtctggc aaactatcca accatatttt caaaaaaatc
      541 gtttagtcgt tgcacataat gctgcttttg atacaaaggt cttagcggga tgcttagatt
      601 attatcatct tgaacagcct aattatcttt ctttatgtac agtgaaaagt agccgtcgcc
      661 tgtttcctga atttccaaat catcgattga acactgtgtg cgaaaatttg aacatccaat
      721 tagatcatca ccatgatgca ttggaagata gtcgtgcatg tgcggaaatc ctgctttatc
      781 aagaaaaaaa attcgggaca gatccattga aaaaacttgt gactataaag taaaagaata
      841 cttatgaaac acacaaagag gtcgtgagcc tgccgtttca ctccgagtca caaggagcaa
      901 tttcaaaaaa tagcttctca tgtttattaa atattgtgac tggtgaccga ggagctggca
      961 ggagaacacc gtttattgga gaggttgtga aaaagctgtt ctgcatcaag taataagaac
     1021 agccaaacaa aaatagcttc tcatattttt catttggttg gacttattac cgcagatgca
     1081 agcttttgga caccgtttac c
//
LOCUS       DACGLH010000197         1078 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.209, whole genome shotgun sequence.
ACCESSION   DACGLH010000197 DACGLH010000000
VERSION     DACGLH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.209"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            298..507
                     /locus_tag="IXT21_003030"
     CDS             298..507
                     /locus_tag="IXT21_003030"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ4870364.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHGLYEKA"
     gene            complement(479..892)
                     /locus_tag="IXT21_003031"
                     /pseudo
     CDS             complement(479..892)
                     /locus_tag="IXT21_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(962..>1078)
                     /locus_tag="IXT21_003032"
     CDS             complement(962..>1078)
                     /locus_tag="IXT21_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ4870365.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          327 a          217 c          184 g          350 t
ORIGIN      
        1 ttattctttt cataaatatt gtaaatagaa gtattaaaaa tatatacaaa aagtattatt
       61 tcgagaaaga taaatatttt caaaaacctt ttcataatcc tcaccctttc ttacactgat
      121 aaatcatgcg tctaatattc aaattatact cgatatgctt tagctaatca gccaactctg
      181 tttgtctact atcattatat caatagacaa tatctgctcc cgtctttatg atcgtgccaa
      241 agcaatcaaa agaaaattgc gaatcactta gatcgaaagt ttactcaaac aaaagcattg
      301 tcagcggttg tcacggattt aacttatgtt cgggtcggga caaattgggc ttatatttgt
      361 ttgatgattg atttattcaa tcgtgaaatt attggccatt cttgcggaaa taaaaaagat
      421 gcgcagcttg taaaaagggc cattcaaagc attccttact cacttcaaga aatcgaactc
      481 ttccacggtc tgtatgaaaa agcttgacgt ctgttaaaga atacggtatc cgtccaaagg
      541 cttcttttac caatgaggca tctttcttct caccacagga ataaccaata atttctcgat
      601 taaacaaatc aagtattaag cagatataat gccacttttt ccccacgcga acataagtaa
      661 gatccgtaac gatggcttcc aatggctgtt cttgtgtaaa ggtccgatct aatacattcg
      721 ctgttttcgc ttcattacaa gctgttcgtt gccctttaaa atgagcgatc gtataggtag
      781 atgtcaatcc gcggcgtttc atgattcgac cgattcggcg ccgactgagt tgaagcccac
      841 gctttgctaa acacttcttc aattttcggg taccgtaagc ctttcggttt cgcttgataa
      901 tagtaggggt tctgttgcaa agttttaaat aaagaataaa atcccttacg gtatctatga
      961 tttaagctgg gattcccaat aataccttga tttcagtaca gaccgaaaac ccgaagagag
     1021 tgccttcttt tcgggttttc ttatataatc ctcggatggc ttccatgcct ttaatcgt
//
LOCUS       DACGLH010000198         1061 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.210, whole genome shotgun sequence.
ACCESSION   DACGLH010000198 DACGLH010000000
VERSION     DACGLH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.210"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(36..992)
                     /locus_tag="IXT21_003033"
     CDS             complement(36..992)
                     /locus_tag="IXT21_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870366.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACGLH010000199         1045 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.211, whole genome shotgun sequence.
ACCESSION   DACGLH010000199 DACGLH010000000
VERSION     DACGLH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.211"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            6..980
                     /locus_tag="IXT21_003034"
     CDS             6..980
                     /locus_tag="IXT21_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ4870367.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DACGLH010000200         1008 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.212, whole genome shotgun sequence.
ACCESSION   DACGLH010000200 DACGLH010000000
VERSION     DACGLH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.212"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..>1008
                     /locus_tag="IXT21_003035"
     CDS             <1..>1008
                     /locus_tag="IXT21_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870368.1"
                     /translation="YDYSGNPNLMSKLKSSDFNVGYHGSLTLDNEKLHFTSDGTGSII
                     MFTRINTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITT
                     DDVGKEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQ
                     PNLLAEPYNMCREYPNENIANPTVKFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTK
                     PASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTFTPTKLEPGLPKDLRILQSPKETASA
                     CQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLEPSYVENNLATDIKVAE
                     IT"
BASE COUNT          366 a          187 c          197 g          258 t
ORIGIN      
        1 ctatgattat agtgggaatc cgaatttaat gtccaaacta aaatcgagcg attttaacgt
       61 tggttaccac gggtcactaa ctttggataa cgaaaagtta cattttactt ctgatggtac
      121 aggaagcatt attatgttta cgcgtattaa tacacctcag cttgctagtg ggaaaaccta
      181 tactctgagt gcgaaagttc gatttgatga aggaactaca ggagctattg ataaattacg
      241 tttggtgtat cgtacatcac caggagaaaa gatattattg gaagcaaata gtacaaatat
      301 tacaacagat gatgtaggga aagaaataac aatcaaaggt acagctaacg ttaattatca
      361 aatcacaaat ttagatcgat tttatatgag tattagcttt gttgacaggg ataaaataaa
      421 tggcggattt aagttgtacg acatcaaaat cgaagaaggc tcaacagcca ccccatatca
      481 gccaaactta ttagcagagc cttacaacat gtgtcgcgaa tatcctaacg agaatattgc
      541 caaccctaca gttaagttcc caattaaatc tagcgcgtac gaaatatata aaggtaacac
      601 ggaagaagag cttataatag gtcaaacgta tactatcacg cttaaaggaa caaaacccgc
      661 aagtcaaacc tttgtagcat ataattattg gaacgttaat tttggagacc taaaaccagt
      721 tgaaggattg acagacgtat ggtctctaac attcacacca acgaaacttg aaccgggttt
      781 gcctaaagac cttcgcattt tgcagtcgcc taaagaaacg gccagcgcat gccaaattga
      841 ctggctcaag attgaaaaag gcgacacacg aaccccgaat attgagcaat ataaataccg
      901 aggaatcggc atgcgagact caaacaatcc aaaagattat gtatgggatc tagaacctag
      961 ctatgttgag aataacctag ctacagatat caaggttgct gaaatcac
//
LOCUS       DACGLH010000201         1001 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.213, whole genome shotgun sequence.
ACCESSION   DACGLH010000201 DACGLH010000000
VERSION     DACGLH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.213"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..>1001
                     /locus_tag="IXT21_003036"
     rRNA            <1..>1001
                     /locus_tag="IXT21_003036"
                     /product="23S ribosomal RNA"
BASE COUNT          311 a          198 c          288 g          204 t
ORIGIN      
        1 agctatgatc cagagatttc cgaatggggg aacccaacat ctttaatagg atgttacgat
       61 tgtgtgaata catagcacat tcgaggtaga cgcagagaac tgaaacatct aagtacctgc
      121 aggaagagaa agaaaattcg attccctgag tagcggcgag cgaaacggga aaagcccaaa
      181 ccagcaagct tgcttgttgg ggttgtagga ctccaatatg gtagttcttt cagatagtcg
      241 aatgacttgg aaaagtcagt caaagagggt aaaaaccccg tagacgaaat gtggaagaca
      301 cctaggagga tcctgagtac ggcggaacac gagaaattcc gtcggaatcc gggaggacca
      361 tctcccaagg ctaaatactc cctagtgacc gatagtgaac cagtaccgtg agggaaaggt
      421 gaaaagcacc ccggaagggg agtgaaatag aacctgaaac cgtgtgccta caacaagtca
      481 aagcccgtta atgggtgatg gcgtgccttt tgtagaatga accggcgagt tacgattgca
      541 tgcgaggtta agttgaagag acggagccgc agcgaaagcg agtctgaata gggcgtttga
      601 gtatgtagtc gtagacccga aaccatgtga tctacccatg tccaggttga aggtgcggta
      661 aaacgcactg gaggaccgaa cccacgtacg ttgaaaagtg cggggatgag gtgtgggtag
      721 cggagaaatt ccaaacgaac ttggagatag ctggttctct ccgaaatagc tttagggcta
      781 gcctcggaat tgagaatgat ggaggtagag cactgtttgg actaggggcc catctcgggt
      841 taccgaattc agataaactc cgaatgccat tcattcatat ccgggagtca gactgtgagt
      901 gataagatcc atagtcgaaa gggaaacagc ccagaccacc agctaaggtc ccaaaatata
      961 tgttaagtgg aaaaggatgt ggggttgcac agacaactag g
//
LOCUS       DACGLH010000202         1001 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.214, whole genome shotgun sequence.
ACCESSION   DACGLH010000202 DACGLH010000000
VERSION     DACGLH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.214"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            370..1001
                     /locus_tag="IXT21_003037"
                     /pseudo
     CDS             370..1001
                     /locus_tag="IXT21_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DACGLH010000203          938 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.216, whole genome shotgun sequence.
ACCESSION   DACGLH010000203 DACGLH010000000
VERSION     DACGLH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.216"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            153..554
                     /locus_tag="IXT21_003038"
     CDS             153..554
                     /locus_tag="IXT21_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870369.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            556..927
                     /locus_tag="IXT21_003039"
     CDS             556..927
                     /locus_tag="IXT21_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870370.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          363 a          118 c          170 g          287 t
ORIGIN      
        1 tataaacttt aatataaacg ccagaaatgt cttggttgaa gaagcaggag agcattatgc
       61 tgttgttaaa tttaatggaa ctgagattgg aaggcaaatc tttgatatca aggtcaacaa
      121 accagtggaa aaaaattaag gagacgatac ggatgcttga tatagtacca tcaaatacac
      181 atcggagtgg tagcttaata aattttgctg cgatttttgc ttgcgcatcg gtaattctag
      241 catctccagt tgttgtggat gcaagttcta ctccgaaaac taaaaatgat aatcaacttg
      301 taattactac aaaaaatgag attaacacaa tttcaagtaa tgatggcaaa atttttgatg
      361 tgataaatac ggttttaaaa agaaaagctg aaatcaatat aaaatatgat gaagatttaa
      421 acttgtattt ctttgttatc aaaacaacat ctgaattgtt ttcctcagat tacgatgttt
      481 tggatactct cgataatgta ttatctgact ataaatatat ggggaaatct gtagtagcaa
      541 ctttggggga ataaaatgtt tgactttaat gaatatttat ctatttccaa acaaattgaa
      601 tcagatgata aatataattc taaagaatct tatagaagaa ctgctgtttc aagagcatac
      661 tacagtgctt ttaaaaaatc tgatgagtat ttgaaagaaa attatgatat tatttataat
      721 ggaagtagcg gaaaaggaag ccaccaaacc gtttggaatt tgttttcaac agttaaggaa
      781 ttgaatacct taggaataca taatagtggg tatagaatgt tagagaaaag aaaatgtgct
      841 gactatattc ctaatgaaac cattacgaaa acagatatgg cgctgatgaa tcgagaagca
      901 gaaaaaataa taaacaaact cacctaatga atttcaag
//
LOCUS       DACGLH010000204          891 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.218, whole genome shotgun sequence.
ACCESSION   DACGLH010000204 DACGLH010000000
VERSION     DACGLH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.218"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(602..675)
                     /locus_tag="IXT21_003040"
     tRNA            complement(602..675)
                     /locus_tag="IXT21_003040"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
BASE COUNT          304 a          148 c          154 g          285 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc a
//
LOCUS       DACGLH010000205          863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.219, whole genome shotgun sequence.
ACCESSION   DACGLH010000205 DACGLH010000000
VERSION     DACGLH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.219"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..861
                     /locus_tag="IXT21_003041"
     CDS             <1..861
                     /locus_tag="IXT21_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ4870371.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DACGLH010000206          835 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.220, whole genome shotgun sequence.
ACCESSION   DACGLH010000206 DACGLH010000000
VERSION     DACGLH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.220"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IXT21_003042"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IXT21_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ4870372.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          237 a          159 c          140 g          299 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa
      721 aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa
      781 agatcgttca tgattacttg gatggtaaag gcggaagtga ttatctggca aagaa
//
LOCUS       DACGLH010000207          816 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.221, whole genome shotgun sequence.
ACCESSION   DACGLH010000207 DACGLH010000000
VERSION     DACGLH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.221"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(61..261)
                     /locus_tag="IXT21_003043"
     CDS             complement(61..261)
                     /locus_tag="IXT21_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4870373.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          268 a          135 c          128 g          285 t
ORIGIN      
        1 attttttcgt acaaaaaaac cgtcttttct ttcgaaaaga cggttaaaag tcaattttta
       61 ttaagcttta acaatgtttg ttgcttgagg gccacgttga ccatcttcaa catcgtaaga
      121 cactgcttgg ccttcttcta aagttttgaa gccgtcacct tggatagctg aaaaatgtgc
      181 aaatacatca tttccatctt caccagtgat aaagccaaaa cctttgtctg cgttgaacca
      241 ttttactgta ccgttattca taaataaatt cctcctgata cgtatcaatg tacgtttttt
      301 cgttgcaata aatatttgat aggcaaaagg agttttatct atgtagatat atcgctcaaa
      361 ttacgtttca aaatagatta ctcatttaat atatcatgct ttttctaata acacaaggat
      421 aatgcaatga aatataaaat tcattgtctt ctattgtctt cgaatcgaag aaaaagttga
      481 ataaccgcga ttgattcact ttttgttttc ttgtttattt gcttttatct gcaacttttg
      541 actttaaaat ttcttaacta tgaaaacctt tcaattggtg gtatacttat aaatggaatt
      601 tcgttatcaa cgcgttcgtt gcctttcagc aagtatttaa agaaaggaga atggctatga
      661 cattctataa caaagatata caatatcgag taaccttgga tacagatttg aatctatttg
      721 tcgtttttga caaagaaaat accaatcatg tagcgacagg tgttacgatc gaacaagcag
      781 tgcaagaatt aaaaaaatct gcataaggag tggaaa
//
LOCUS       DACGLH010000208          812 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.222, whole genome shotgun sequence.
ACCESSION   DACGLH010000208 DACGLH010000000
VERSION     DACGLH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.222"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..734
                     /gene="aph(2'')-I"
                     /locus_tag="IXT21_003044"
     CDS             <1..734
                     /gene="aph(2'')-I"
                     /locus_tag="IXT21_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAQ4870374.1"
                     /translation="YAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGT
                     FLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRET
                     IYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGD
                     SGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIV
                     YGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          357 a           53 c          131 g          271 t
ORIGIN      
        1 gttatgcaaa agaaaaagca atatataatt ttttaaatac aaatttagaa actaatgtaa
       61 aaattcctaa tattgaatat tcgtatatta gtgatgaatt atctatacta ggttataaag
      121 aaattaaagg aactttttta acaccagaaa tttattctac tatgtcagaa gaagaacaaa
      181 atttgttaaa acgagatatt gccagttttt taagacaaat gcacggttta gattatacag
      241 atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat atattgttgc
      301 gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa agttttatgg
      361 aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat aatgatttta
      421 gttgtaatca tctattgtta gatggcaata atagattaac tggaataatt gattttggag
      481 attctggaat tatagatgaa tattgtgatt ttatatactt acttgaagat agtgaagaag
      541 aaataggaac aaattttgga gaagatatat taagaatgta tggaaatata gatattgaga
      601 aagcaaaaga atatcaagat atagttgaag aatattatcc tattgaaact attgtttatg
      661 gaattaaaaa tattaaacag gaatttatcg aaaatggtag aaaagaaatt tataaaagga
      721 cttataaaga ttgattatat aatatatgaa aagctattat aaaagacatt agtattaaat
      781 agtttaaaaa aatgaaaaat aataaaggaa gt
//
LOCUS       DACGLH010000209          789 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.223, whole genome shotgun sequence.
ACCESSION   DACGLH010000209 DACGLH010000000
VERSION     DACGLH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.223"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..782
                     /locus_tag="IXT21_003045"
     CDS             <1..782
                     /locus_tag="IXT21_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4870375.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          106 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgccc
//
LOCUS       DACGLH010000210          787 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.224, whole genome shotgun sequence.
ACCESSION   DACGLH010000210 DACGLH010000000
VERSION     DACGLH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.224"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..737
                     /locus_tag="IXT21_003046"
     CDS             <1..737
                     /locus_tag="IXT21_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ4870376.1"
                     /translation="CTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYN
                     AVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKR
                     LSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNN
                     RAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPN
                     LPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          249 a          144 c          171 g          223 t
ORIGIN      
        1 tttgtacagg aaaagacagt cagcatccca tcgtttatta tcaccacgac gaaagtcgtg
       61 ctggagatgt tccgaaaact tttctcaagg agtttacagg gtatttacac tgtgacggct
      121 atagcgggta taatgccgtg gaaagtgtac gacttgtcta ttgtttcgcg catgttcggc
      181 ggaaattttt cgaagcaatt ccaaaaggaa agaaaaacac ggatattccc gcagctcaag
      241 cagtcaaaca attggataaa tggtttgtcc tagaaaagaa atggaaagac ttttctcctg
      301 agaagcgatt aagctgtcgg caacaagaac tgcgtccatt atttattgct ttttatgagt
      361 ggatggcaac gatagatcct gttgcaaaat ccaagttgga tgcagccgtt caatatgcgt
      421 gtaaactacg aagtggtttt gaacctattt ttgaagatgg tcgtttagaa ctcacaaata
      481 atcgtgcgga gcgaaatatc aaagagctgg tgataggacg gaaaaattgg ttgcattcca
      541 caagtcttga aggagcacga acttcaggaa tcattctgag tgtatataaa acagcagaac
      601 ttaacggatt gaatcctgta aagtatctag agtttctatt tgataagata ccaaatcttc
      661 ccgtactttc agcagaaact ttagatcaac tgttgccttg gaacaaggat gtacaacaac
      721 atttttctcg taactaaaaa cgccaaacat tcccgattct attctatagg tcgggttatt
      781 tgacgta
//
LOCUS       DACGLH010000211          740 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.225, whole genome shotgun sequence.
ACCESSION   DACGLH010000211 DACGLH010000000
VERSION     DACGLH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.225"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            176..379
                     /locus_tag="IXT21_003047"
     CDS             176..379
                     /locus_tag="IXT21_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ4870377.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            379..729
                     /locus_tag="IXT21_003048"
     CDS             379..729
                     /locus_tag="IXT21_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4870378.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          299 a           79 c          119 g          243 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttacactct cgtcaatcat
       61 tgttatataa taaaatgtta tataataaaa ttactaatga tatttaatat tacaagcaat
      121 cttaagaaat tcgaaaagga aaaaatgatt atgaaaaaga aagaaaaaga aagtattgaa
      181 acattgtgtt attctaggaa tattaggaac ttgtctagct ggcattggta caggaataga
      241 cgttgacgca gctacttact atggaaatgg tctttattgt aacaaagaaa aatgttgggt
      301 aaattgggga caatcatggt cagaagggtt aaaacgttgg ggagataatc tttttggtag
      361 ttttagtgga ggacgataat gacttcaata aacattgatc acacaaaaaa agtcatacat
      421 gaattataca atagtttaat gacacgacct aatcagacga aagcattatt agatattact
      481 gatgttttat ttcaggtata taaaaaaatt gatacagcaa aatatcctga aattcttgta
      541 aataaattag ttaattatat ttacatcgtg ggttttgata ataaaatacg ctttattgat
      601 aatgatgaaa aattattaat agaattaggg gatatttcta aaaaagctgg aattaatagt
      661 aaatacaaag caaactttac agataaatcg caattttata gttattcaga aacaatgcct
      721 caacgataaa atatagtata
//
LOCUS       DACGLH010000212          731 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.226, whole genome shotgun sequence.
ACCESSION   DACGLH010000212 DACGLH010000000
VERSION     DACGLH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.226"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IXT21_003049"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IXT21_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ4870379.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IXT21_003050"
     CDS             complement(481..675)
                     /locus_tag="IXT21_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870380.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DACGLH010000213          721 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.227, whole genome shotgun sequence.
ACCESSION   DACGLH010000213 DACGLH010000000
VERSION     DACGLH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.227"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(400..714)
                     /locus_tag="IXT21_003051"
     CDS             complement(400..714)
                     /locus_tag="IXT21_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870381.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          280 a          103 c           95 g          243 t
ORIGIN      
        1 acgttgttaa atcaacgttt ttttattttg gtcaaggaga gaaaagtaga gaaatacaaa
       61 attttttgca ttgattttgc attcaaaaaa acattcaaac atttagaaat ccccaatacg
      121 tattcaggaa aatctatttt aaggtttaca aaaagaactt ttgttcgtac actctttttg
      181 aggtgaactt ttatgctgat ggaaggaaaa acacaattat ggtttaagca tgttccttca
      241 aataagtttt ttaattataa actctagaga aattaattca tcctaacata aaataattgt
      301 tcactcaaaa gtttttctag aacaattaaa acagcaggct tcacattcgc tatacatttg
      361 taagaagtaa taattcctgt actaaaaggc ataattgctt taattatttt ttgacttctc
      421 ttcaaacaca agaagtagtt gtgtaataat tgcagaaata aggattccaa taatagatat
      481 aaaatttgac atggaaacgt ctataacatt aaaagaataa agtgttgaag aaagtaaact
      541 gattgaaata acccctccgt aaaaaatagt ctgtgaatat acccactttt tttcagaaga
      601 cgtacttcgt ttggttctaa aacctatccc tgcatttcta ctcttatcaa aaaaccaaat
      661 atttagaagt aataatattg ctattattaa gataaaatta ctgtaaaaat ccaaaagcta
      721 t
//
LOCUS       DACGLH010000214          705 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.228, whole genome shotgun sequence.
ACCESSION   DACGLH010000214 DACGLH010000000
VERSION     DACGLH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.228"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
BASE COUNT          238 a          114 c          105 g          248 t
ORIGIN      
        1 acagctaaca aaattgtcat aaaaaacgag atggttgata ttagaacttg tgtttttgtt
       61 taggtctaga gataaagaga gacaatttcc aaatatatgt aatgaataca aaaagacgtt
      121 tgtcgcggat taccgttaca aacgtctttt attgattttc cgagatctca tcagtcgcca
      181 tttggaatgg ttcactcctt atctagattc gtttaatgct cggactgctt gttcgattgt
      241 aataccaaac gcaacgttat tttcgttttg tgcatcaatc gccataaaac ggttagaagt
      301 tgtatcaaaa gctacgcggt attgcatatt tgtttttgta aacgtcataa aattcatctc
      361 ctttgtaatg taatttagtt cttgaaagtt tcctttaaac tttcgaactt ataccaagtt
      421 tagcatcgta cgttttttga tgcaattaaa aagcatttat cattttcctt atttattttt
      481 tcttaaatgg tttagctatt ttcttttaat ccgataagta gcccaatcat caaggttaca
      541 aatactattg cttgaatata aatcatatgc tcacctcttt ataaataact tagcaatata
      601 aagaaaaaaa cgcacgaaca acataggaat ataaacaaac gtttttgtca caacgtctaa
      661 gatcgtgtta aaaaatagtt atctaaaaag cagaaagata ctcaa
//
LOCUS       DACGLH010000215          689 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.229, whole genome shotgun sequence.
ACCESSION   DACGLH010000215 DACGLH010000000
VERSION     DACGLH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.229"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..477
                     /gene="scm"
                     /locus_tag="IXT21_003052"
     CDS             <1..477
                     /gene="scm"
                     /locus_tag="IXT21_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ4870382.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          249 a          147 c          116 g          177 t
ORIGIN      
        1 agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc
       61 acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagctca
      121 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
      181 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
      241 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
      301 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
      361 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
      421 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
      481 aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt
      541 tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag
      601 aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg
      661 taagggaaaa gagattctct tttgttcga
//
LOCUS       DACGLH010000216          687 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.230, whole genome shotgun sequence.
ACCESSION   DACGLH010000216 DACGLH010000000
VERSION     DACGLH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.230"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            45..380
                     /locus_tag="IXT21_003053"
     CDS             45..380
                     /locus_tag="IXT21_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870383.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          263 a           77 c          117 g          230 t
ORIGIN      
        1 caatttccta ggaaaaatag ttaacataaa aggaatgaat tgttatgaaa caatatttaa
       61 atgaatggaa agcgatagag gggtcattag taacgcaaag aataaaggaa ttacctgatt
      121 gcttagagaa ggatcatttg tttcaaatac gagaaatgtt gaaaaaagaa cagtttgatc
      181 cagaccaatt tttagtagtc gagtatccaa ctattggagt gtactgttgt aaccatatca
      241 atgatggaaa atattttatt attcaggagt atggaggaca gttaactcct ttctatacta
      301 catgggaaat gagtgaagaa ggaatcaata attttccatg tgaaagcatc gaagattcaa
      361 tttcacacac cgaatgttaa ttgattataa ctataaaaaa ggattgaaca ttatgttcaa
      421 tccttttttc aatgaaatgt tttccgccat ttcattgttt ggtgcgattt ctattttatt
      481 atatcaaaat aaagagagat tctaaagaaa gaagagggaa atatataaat caagcgacag
      541 tatatttatt attaaatgct attgtgagaa tataactaat tagtttaatt tgaaagtaaa
      601 tatacgctat aaaagtgcat cttgtgttat aaaaaattat tttattttgt gaacataatt
      661 taacaattct ttctcggaat tgatttc
//
LOCUS       DACGLH010000217          676 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.231, whole genome shotgun sequence.
ACCESSION   DACGLH010000217 DACGLH010000000
VERSION     DACGLH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.231"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..526)
                     /locus_tag="IXT21_003054"
     CDS             complement(<1..526)
                     /locus_tag="IXT21_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870384.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRK"
     gene            complement(532..>676)
                     /locus_tag="IXT21_003055"
     CDS             complement(532..>676)
                     /locus_tag="IXT21_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ4870385.1"
                     /translation="LIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRKEL
                     DKK"
BASE COUNT          180 a          144 c          119 g          233 t
ORIGIN      
        1 gctttcgtct tcctagcgct ttgtcaaagt gatcgatgtc agctcctgtt ttgccacaga
       61 tgcagcagac tctttttgtg atgcatttgt agaagtaata ttcttgattc gcaggtaaaa
      121 tctcatagcc ttctttgaaa ggaatatgat gttcaaagat gaaatctaag atgatatttg
      181 ctaagacatt ggcatcactc acagttgtat tcgattcgtc tttcaggctt attttgcgcc
      241 ctgtgacacc ttcaaaacga aagtagaaga attccttcca gaagtccgtt ggcatgcctg
      301 tatcgataaa aatatcgcct atcagcgcat agatgaagtt tcgttgctgt acggtgaaac
      361 gtctaggatc aataaaacga atttcaatga ctcgatcacc atcgtagccg tcatacatcg
      421 tctttagtcg atcaatgttc acttcctcat tgatggttgc gcttatgtct tttcctttga
      481 actttttcag aaccgctgaa tatgaatcga ttaatggttt aaacactcat atcacttctt
      541 atctaattct tttctcttag ctgctattgc tcgctccatc aaggcacatt gctcatagct
      601 taactgttca atagtttcaa cgttatcagc taagagccct aatttatctg tctgctcatt
      661 aacatattcg attaag
//
LOCUS       DACGLH010000218          649 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.233, whole genome shotgun sequence.
ACCESSION   DACGLH010000218 DACGLH010000000
VERSION     DACGLH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.233"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..>649
                     /locus_tag="IXT21_003056"
     CDS             <1..>649
                     /locus_tag="IXT21_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4870386.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DACGLH010000219          638 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.234, whole genome shotgun sequence.
ACCESSION   DACGLH010000219 DACGLH010000000
VERSION     DACGLH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.234"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..525
                     /gene="tnpB"
                     /locus_tag="IXT21_003057"
     CDS             <1..525
                     /gene="tnpB"
                     /locus_tag="IXT21_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ4870387.1"
                     /translation="KMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLH
                     EKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQ
                     YKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASIN
                     ILTEGLRLHSMGLA"
BASE COUNT          238 a          101 c          123 g          176 t
ORIGIN      
        1 aaaatggaaa agaaactaaa gcgtgaacaa cgcaaattgt ctaggcgtgc tttattggct
       61 aaaaataaag gtaccaatct atttgaagct aaaaattatc aaaaacaaaa acgcaaagta
      121 gctagattgc atgaaaaagt aatgaatcaa cgtactgatt ttctgaataa gttgagtaca
      181 gaaatgatca aaaatcacga tattatctgt attgaagact taaacacaaa aggtatgttg
      241 cgaaatcata aattagctaa aagtatttct gatgtttctt ggtcaaagtt tgtgactaaa
      301 ttacaataca aggctgactg gtatggacga aaaatcatca aagtagataa atggtttccg
      361 tctagtcaga tttgttcaga gtgcggacat aaagatggca agaaatctct tgaaattcga
      421 gaatggactt gtcctgtttg tcatactcat catgatcgtg atatcaatgc tagtatcaat
      481 attctgaccg aaggtctaag actacattct atgggattgg cttagatcca atcatcaaga
      541 accgtaggaa ctacggggat agcttggtaa ataagagaca ccgctgttag taaagaaata
      601 cgctatcaag tatgctctat tcccaagaag ctcccact
//
LOCUS       DACGLH010000220          573 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.235, whole genome shotgun sequence.
ACCESSION   DACGLH010000220 DACGLH010000000
VERSION     DACGLH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.235"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..442)
                     /locus_tag="IXT21_003058"
     CDS             complement(<1..442)
                     /locus_tag="IXT21_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAQ4870388.1"
                     /translation="MSDKQKKRYYWLKLKEDFFEEDTIEWLEEQPNGKEYCLFYLKLC
                     LKSLKTEGILVRNVGNLMIPYDPESLAKLTNSNTDTVKVAMDLFNKIGLIKILDSGEI
                     YLNQLSELVGSETEYARQKRIQRAKEDNVQKLSGKGRLEIELEKD"
BASE COUNT          132 a          117 c           84 g          240 t
ORIGIN      
        1 tatctttctc taactctatc tctaggcgac cttttccaga caacttctgg acattgtcct
       61 cctttgctct ttggattcgt ttttgcctag catattcagt ctctgatcct actaattcac
      121 ttagctgatt tagatatatc tctccgctgt ccagtatttt tattagtccg attttgttga
      181 atagatccat agcgactttc actgtgtccg tatttgaatt tgttagtttt gctagagatt
      241 cgggatcgta aggtatcatc agattaccta catttctaac tagtattcct tctgtcttta
      301 gagatttaag acagagtttc agataaaata agcaatattc tttaccattg ggttgttctt
      361 ccaaccattc gatggtatct tcttcgaaaa aatcttcttt taatttaagc cagtaatagc
      421 gtttcttttg tttgtctgac aattcagtac cacctttccc ttatcctccg atatttaact
      481 ttttgattgt ctcctgattt aacttaatcc ctttgatttg atatttattt ttgaaattga
      541 tcacacctat cttgtgcttc tctgtgtgat gga
//
LOCUS       DACGLH010000221          573 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.236, whole genome shotgun sequence.
ACCESSION   DACGLH010000221 DACGLH010000000
VERSION     DACGLH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.236"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
BASE COUNT          185 a           81 c           81 g          226 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct ttt
//
LOCUS       DACGLH010000222          565 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.237, whole genome shotgun sequence.
ACCESSION   DACGLH010000222 DACGLH010000000
VERSION     DACGLH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.237"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            51..>565
                     /locus_tag="IXT21_003059"
     CDS             51..>565
                     /locus_tag="IXT21_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ4870389.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRP"
BASE COUNT          205 a           90 c          112 g          158 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtcc
//
LOCUS       DACGLH010000223          555 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.238, whole genome shotgun sequence.
ACCESSION   DACGLH010000223 DACGLH010000000
VERSION     DACGLH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.238"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..>555
                     /locus_tag="IXT21_003060"
     CDS             <1..>555
                     /locus_tag="IXT21_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4870390.1"
                     /translation="KRSTEGWTPDVIIGRAERTFSCSVSTLYRRFKTGEFNVLHLPMQ
                     GKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEFGHLEGDTIVGIHHKSAVITLVER
                     LSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSISNTNDID
                     IYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          193 a          105 c          112 g          145 t
ORIGIN      
        1 aaaaagatca actgaaggtt ggaccccaga cgtcattatt ggccgtgcag agcgaacctt
       61 ctcttgttca gtaagtaccc tctatcgccg gtttaagacg ggagaattca atgttttaca
      121 tttaccgatg caaggaaaac gaaaaccaaa tggttataag gaaaaacgtg gaaaacaggc
      181 attcaaaaga aatatttctg agcgtaaaaa agattatgtc gttttcgaag aagaatttgg
      241 acatttagag ggtgatacga ttgtcggcat ccaccataaa agtgccgtca tcacactcgt
      301 tgagcgactt tcaaaagcca ttatcgcctt gaaaccagaa ggccgtaagg cagttgatat
      361 tgaaaattcg attaatgaat ggcttcaatc cgtacccaaa aatcttttca aatcaattac
      421 ctttgattgt ggaaaagaat tctctaattg gaaaagtatt agtaatacga atgatattga
      481 tatttatttc gcagacccag gaacgccttc ccaacgggga ttaaatgaac attcaaacgg
      541 actcctccga aaaga
//
LOCUS       DACGLH010000224          550 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.239, whole genome shotgun sequence.
ACCESSION   DACGLH010000224 DACGLH010000000
VERSION     DACGLH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.239"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            67..>550
                     /locus_tag="IXT21_003061"
     CDS             67..>550
                     /locus_tag="IXT21_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ4870391.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     I"
BASE COUNT          197 a           88 c          109 g          156 t
ORIGIN      
        1 acttgattga tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag
       61 aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag tcgtaagtta
      121 aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca cgcgtgggag
      181 attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtacaa acactacagg
      241 gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt tcttatggat
      301 gaaactggga cttatgtatc tgtctcagta acggttaaag gacacacgga aaccgaatta
      361 tttcctgttt tagattatcg aaaccaacca gttgttaaac caaatgcaat gcaaatcaat
      421 aactcattga agcgatgctt tgtgaaagca ttggctctac acggactggg attatatgta
      481 tttcaagggg aagatattcc aacaccacct agaatcgata caaagaaatt aaacatgcta
      541 gagacgattc
//
LOCUS       DACGLH010000225          548 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.240, whole genome shotgun sequence.
ACCESSION   DACGLH010000225 DACGLH010000000
VERSION     DACGLH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.240"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..>548)
                     /locus_tag="IXT21_003062"
     CDS             complement(<1..>548)
                     /locus_tag="IXT21_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4870392.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DACGLH010000226          543 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.241, whole genome shotgun sequence.
ACCESSION   DACGLH010000226 DACGLH010000000
VERSION     DACGLH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.241"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(136..426)
                     /locus_tag="IXT21_003063"
     CDS             complement(136..426)
                     /locus_tag="IXT21_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870393.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
BASE COUNT          231 a           88 c           78 g          146 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agt
//
LOCUS       DACGLH010000227          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.242, whole genome shotgun sequence.
ACCESSION   DACGLH010000227 DACGLH010000000
VERSION     DACGLH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.242"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(5..90)
                     /locus_tag="IXT21_003064"
     tRNA            complement(5..90)
                     /locus_tag="IXT21_003064"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IXT21_003065"
     tRNA            complement(107..178)
                     /locus_tag="IXT21_003065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IXT21_003066"
     tRNA            complement(190..262)
                     /locus_tag="IXT21_003066"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IXT21_003067"
     tRNA            complement(280..361)
                     /locus_tag="IXT21_003067"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IXT21_003068"
     tRNA            complement(369..441)
                     /locus_tag="IXT21_003068"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IXT21_003069"
     tRNA            complement(445..517)
                     /locus_tag="IXT21_003069"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACGLH010000228          517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.243, whole genome shotgun sequence.
ACCESSION   DACGLH010000228 DACGLH010000000
VERSION     DACGLH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.243"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            33..>517
                     /locus_tag="IXT21_003070"
     CDS             33..>517
                     /locus_tag="IXT21_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ4870394.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     F"
BASE COUNT          191 a           95 c           84 g          147 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttat
//
LOCUS       DACGLH010000229          481 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.244, whole genome shotgun sequence.
ACCESSION   DACGLH010000229 DACGLH010000000
VERSION     DACGLH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.244"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..449)
                     /locus_tag="IXT21_003071"
     CDS             complement(<1..449)
                     /locus_tag="IXT21_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4870395.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYLK"
BASE COUNT          136 a           94 c           61 g          190 t
ORIGIN      
        1 ttttttaaat atgcgttctc tgctcttaaa cgaataactt cttcttcaag agactcatct
       61 ttaagttttt taggaatgtt tagcttggaa ttcatactaa tttttctgcc ccttttttgg
      121 ctctcaagtg aagaagcacc gccttcttca tattgctcta tccatttact tagagttcta
      181 tttgaaccga tattaaattt tttagcagtt tcttggatag aaagaccatt tgtttccata
      241 tattctataa catcaagttt aaattttgta gtgtagcttt tgccacctcc aaccaagcct
      301 tcccaaccat gatagttata aatccttacc caatgtctaa ccagtgtacg atttatttga
      361 tatttatgtg caagatattt gtagccgcct tcgttattta aatagtctga aactactttt
      421 ttcttaaaaa caaatgtata tttccgcaaa aaaagcaccc cttttaatta gatttctagt
      481 c
//
LOCUS       DACGLH010000230          478 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.245, whole genome shotgun sequence.
ACCESSION   DACGLH010000230 DACGLH010000000
VERSION     DACGLH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.245"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(72..>478)
                     /locus_tag="IXT21_003072"
     CDS             complement(72..>478)
                     /locus_tag="IXT21_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ4870396.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          151 a           95 c           72 g          160 t
ORIGIN      
        1 acacgaccta aagatcgtgt ctaattatga ctgtatcaga aaatatgata ccaaacggat
       61 ctattcatta attaattgac atagtttatc tgttgctctt ttatgactgg ataactgacc
      121 aattcatgac gatccaagtc ttctttattc atatttacag cagtaatttg actgtcttca
      181 tacgtacgat aataataaat ccctttatca acgttacagc aagaagaata aatcgtatat
      241 tcgtattttc cttctccagt atcgcatact cctttttgtt gctcgactga gcctagtata
      301 tgaaaaaatt gactgatact ttcagattct gaatcacctg atgcagcatt cattttagta
      361 aatgtggctt ttacaaatcg agaaacagaa gagagatctc caggtaagcc caatcctccc
      421 atcccacgac tataaacatt caagctgatc tggtttgaaa aatgattttc tggtgttc
//
LOCUS       DACGLH010000231          474 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.246, whole genome shotgun sequence.
ACCESSION   DACGLH010000231 DACGLH010000000
VERSION     DACGLH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.246"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..>474)
                     /locus_tag="IXT21_003073"
     CDS             complement(<1..>474)
                     /locus_tag="IXT21_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ4870397.1"
                     /translation="YTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMDMFNSE
                     ILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIF
                     QSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIK"
BASE COUNT          137 a           90 c           82 g          165 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg taga
//
LOCUS       DACGLH010000232          464 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.247, whole genome shotgun sequence.
ACCESSION   DACGLH010000232 DACGLH010000000
VERSION     DACGLH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.247"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..>464
                     /locus_tag="IXT21_003074"
     CDS             <1..>464
                     /locus_tag="IXT21_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ4870398.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSY"
BASE COUNT          160 a           81 c           96 g          127 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttat
//
LOCUS       DACGLH010000233          460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.denovo.248, whole genome shotgun sequence.
ACCESSION   DACGLH010000233 DACGLH010000000
VERSION     DACGLH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.denovo.248"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(20..91)
                     /locus_tag="IXT21_003075"
     tRNA            complement(20..91)
                     /locus_tag="IXT21_003075"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IXT21_003076"
     tRNA            complement(113..200)
                     /locus_tag="IXT21_003076"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IXT21_003077"
     tRNA            complement(225..298)
                     /locus_tag="IXT21_003077"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IXT21_003078"
     tRNA            complement(312..382)
                     /locus_tag="IXT21_003078"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IXT21_003079"
     tRNA            complement(384..459)
                     /locus_tag="IXT21_003079"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACGLH010000234        83079 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.005, whole genome shotgun sequence.
ACCESSION   DACGLH010000234 DACGLH010000000
VERSION     DACGLH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.005"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            40..288
                     /locus_tag="IXT21_003080"
                     /pseudo
     CDS             40..288
                     /locus_tag="IXT21_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            347..1207
                     /locus_tag="IXT21_003081"
     CDS             347..1207
                     /locus_tag="IXT21_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ4870399.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            1314..3707
                     /locus_tag="IXT21_003082"
     CDS             1314..3707
                     /locus_tag="IXT21_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4870400.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            3889..5730
                     /locus_tag="IXT21_003083"
     CDS             3889..5730
                     /locus_tag="IXT21_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ4870401.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            5754..6863
                     /gene="rpoD"
                     /locus_tag="IXT21_003084"
     CDS             5754..6863
                     /gene="rpoD"
                     /locus_tag="IXT21_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ4870402.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            7083..9182
                     /locus_tag="IXT21_003085"
     CDS             7083..9182
                     /locus_tag="IXT21_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ4870403.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            9378..10241
                     /locus_tag="IXT21_003086"
     CDS             9378..10241
                     /locus_tag="IXT21_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4870404.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            10346..10819
                     /locus_tag="IXT21_003087"
     CDS             10346..10819
                     /locus_tag="IXT21_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4870405.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            10915..11802
                     /gene="xerD"
                     /locus_tag="IXT21_003088"
     CDS             10915..11802
                     /gene="xerD"
                     /locus_tag="IXT21_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ4870406.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            11821..12192
                     /locus_tag="IXT21_003089"
     CDS             11821..12192
                     /locus_tag="IXT21_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ4870407.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            12162..12959
                     /locus_tag="IXT21_003090"
     CDS             12162..12959
                     /locus_tag="IXT21_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ4870408.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            12943..13512
                     /gene="scpB"
                     /locus_tag="IXT21_003091"
     CDS             12943..13512
                     /gene="scpB"
                     /locus_tag="IXT21_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ4870409.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            13518..14234
                     /locus_tag="IXT21_003092"
     CDS             13518..14234
                     /locus_tag="IXT21_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ4870410.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      14316..14441
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            14564..15175
                     /locus_tag="IXT21_003093"
     CDS             14564..15175
                     /locus_tag="IXT21_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4870411.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            15553..17076
                     /locus_tag="IXT21_003094"
     CDS             15553..17076
                     /locus_tag="IXT21_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ4870412.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            17313..18653
                     /locus_tag="IXT21_003095"
     CDS             17313..18653
                     /locus_tag="IXT21_003095"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ4870413.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            18806..19543
                     /locus_tag="IXT21_003096"
     CDS             18806..19543
                     /locus_tag="IXT21_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ4870414.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(19708..19929)
                     /locus_tag="IXT21_003097"
     CDS             complement(19708..19929)
                     /locus_tag="IXT21_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ4870415.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            19960..20991
                     /locus_tag="IXT21_003098"
     CDS             19960..20991
                     /locus_tag="IXT21_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870416.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            20978..22381
                     /locus_tag="IXT21_003099"
     CDS             20978..22381
                     /locus_tag="IXT21_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ4870417.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            22447..23082
                     /locus_tag="IXT21_003100"
     CDS             22447..23082
                     /locus_tag="IXT21_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4870418.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            23131..23811
                     /locus_tag="IXT21_003101"
     CDS             23131..23811
                     /locus_tag="IXT21_003101"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ4870419.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            23946..25175
                     /gene="rpsA"
                     /locus_tag="IXT21_003102"
     CDS             23946..25175
                     /gene="rpsA"
                     /locus_tag="IXT21_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ4870420.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            25357..26667
                     /locus_tag="IXT21_003103"
     CDS             25357..26667
                     /locus_tag="IXT21_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ4870421.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            26910..27185
                     /locus_tag="IXT21_003104"
     CDS             26910..27185
                     /locus_tag="IXT21_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ4870422.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            27420..28466
                     /locus_tag="IXT21_003105"
     CDS             27420..28466
                     /locus_tag="IXT21_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870423.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            28468..29727
                     /locus_tag="IXT21_003106"
     CDS             28468..29727
                     /locus_tag="IXT21_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ4870424.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            29733..30314
                     /locus_tag="IXT21_003107"
     CDS             29733..30314
                     /locus_tag="IXT21_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ4870425.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(30396..31271)
                     /locus_tag="IXT21_003108"
     CDS             complement(30396..31271)
                     /locus_tag="IXT21_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4870426.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            31419..31751
                     /locus_tag="IXT21_003109"
     CDS             31419..31751
                     /locus_tag="IXT21_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ4870427.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            31863..33071
                     /locus_tag="IXT21_003110"
     CDS             31863..33071
                     /locus_tag="IXT21_003110"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ4870428.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            33225..33680
                     /locus_tag="IXT21_003111"
     CDS             33225..33680
                     /locus_tag="IXT21_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870429.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            34042..34317
                     /locus_tag="IXT21_003112"
     CDS             34042..34317
                     /locus_tag="IXT21_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870430.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            34330..36408
                     /locus_tag="IXT21_003113"
     CDS             34330..36408
                     /locus_tag="IXT21_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ4870431.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            36568..38454
                     /locus_tag="IXT21_003114"
     CDS             36568..38454
                     /locus_tag="IXT21_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4870432.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            38466..39413
                     /locus_tag="IXT21_003115"
     CDS             38466..39413
                     /locus_tag="IXT21_003115"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ4870433.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            39433..39960
                     /locus_tag="IXT21_003116"
     CDS             39433..39960
                     /locus_tag="IXT21_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4870434.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(40023..40676)
                     /locus_tag="IXT21_003117"
     CDS             complement(40023..40676)
                     /locus_tag="IXT21_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ4870435.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            40808..41650
                     /locus_tag="IXT21_003118"
     CDS             40808..41650
                     /locus_tag="IXT21_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4870436.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            41808..42704
                     /locus_tag="IXT21_003119"
     CDS             41808..42704
                     /locus_tag="IXT21_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4870437.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            42707..43420
                     /locus_tag="IXT21_003120"
     CDS             42707..43420
                     /locus_tag="IXT21_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ4870438.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            43440..43958
                     /gene="msrA"
                     /locus_tag="IXT21_003121"
     CDS             43440..43958
                     /gene="msrA"
                     /locus_tag="IXT21_003121"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4870439.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            43955..44182
                     /locus_tag="IXT21_003122"
     CDS             43955..44182
                     /locus_tag="IXT21_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ4870440.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            44334..44885
                     /gene="lepB"
                     /locus_tag="IXT21_003123"
     CDS             44334..44885
                     /gene="lepB"
                     /locus_tag="IXT21_003123"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4870441.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(44968..45510)
                     /locus_tag="IXT21_003124"
     CDS             complement(44968..45510)
                     /locus_tag="IXT21_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ4870442.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(45747..46145)
                     /locus_tag="IXT21_003125"
     CDS             complement(45747..46145)
                     /locus_tag="IXT21_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4870443.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            46405..47265
                     /gene="ylqF"
                     /locus_tag="IXT21_003126"
     CDS             46405..47265
                     /gene="ylqF"
                     /locus_tag="IXT21_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ4870444.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            47258..48025
                     /locus_tag="IXT21_003127"
     CDS             47258..48025
                     /locus_tag="IXT21_003127"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ4870445.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            48081..48938
                     /gene="dprA"
                     /locus_tag="IXT21_003128"
     CDS             48081..48938
                     /gene="dprA"
                     /locus_tag="IXT21_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ4870446.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            49060..51138
                     /gene="topA"
                     /locus_tag="IXT21_003129"
     CDS             49060..51138
                     /gene="topA"
                     /locus_tag="IXT21_003129"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ4870447.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            51107..52483
                     /gene="trmFO"
                     /locus_tag="IXT21_003130"
     CDS             51107..52483
                     /gene="trmFO"
                     /locus_tag="IXT21_003130"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ4870448.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            52681..53586
                     /gene="xerC"
                     /locus_tag="IXT21_003131"
     CDS             52681..53586
                     /gene="xerC"
                     /locus_tag="IXT21_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ4870449.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            53623..54171
                     /gene="hslV"
                     /locus_tag="IXT21_003132"
     CDS             53623..54171
                     /gene="hslV"
                     /locus_tag="IXT21_003132"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ4870450.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            54185..55585
                     /gene="hslU"
                     /locus_tag="IXT21_003133"
     CDS             54185..55585
                     /gene="hslU"
                     /locus_tag="IXT21_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ4870451.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            55603..56394
                     /gene="codY"
                     /locus_tag="IXT21_003134"
     CDS             55603..56394
                     /gene="codY"
                     /locus_tag="IXT21_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ4870452.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            56476..57351
                     /locus_tag="IXT21_003135"
     CDS             56476..57351
                     /locus_tag="IXT21_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ4870453.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(57483..58121)
                     /gene="plsY"
                     /locus_tag="IXT21_003136"
     CDS             complement(57483..58121)
                     /gene="plsY"
                     /locus_tag="IXT21_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ4870454.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            58248..58682
                     /locus_tag="IXT21_003137"
     CDS             58248..58682
                     /locus_tag="IXT21_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ4870455.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            58848..60893
                     /gene="parE"
                     /locus_tag="IXT21_003138"
     CDS             58848..60893
                     /gene="parE"
                     /locus_tag="IXT21_003138"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ4870456.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            60905..63355
                     /gene="parC"
                     /locus_tag="IXT21_003139"
     CDS             60905..63355
                     /gene="parC"
                     /locus_tag="IXT21_003139"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ4870457.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            63632..65863
                     /gene="pflB"
                     /locus_tag="IXT21_003140"
     CDS             63632..65863
                     /gene="pflB"
                     /locus_tag="IXT21_003140"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ4870458.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            66107..66868
                     /gene="pflA"
                     /locus_tag="IXT21_003141"
     CDS             66107..66868
                     /gene="pflA"
                     /locus_tag="IXT21_003141"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ4870459.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            66953..67879
                     /locus_tag="IXT21_003142"
     CDS             66953..67879
                     /locus_tag="IXT21_003142"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ4870460.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            68061..68954
                     /locus_tag="IXT21_003143"
     CDS             68061..68954
                     /locus_tag="IXT21_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ4870461.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            69088..69213
                     /locus_tag="IXT21_003144"
     CDS             69088..69213
                     /locus_tag="IXT21_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4870462.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(69347..69979)
                     /locus_tag="IXT21_003145"
     CDS             complement(69347..69979)
                     /locus_tag="IXT21_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870463.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(70127..70669)
                     /locus_tag="IXT21_003146"
     CDS             complement(70127..70669)
                     /locus_tag="IXT21_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ4870464.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            70804..71193
                     /locus_tag="IXT21_003147"
     CDS             70804..71193
                     /locus_tag="IXT21_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870465.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            71197..71961
                     /locus_tag="IXT21_003148"
     CDS             71197..71961
                     /locus_tag="IXT21_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4870466.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            72090..73037
                     /locus_tag="IXT21_003149"
     CDS             72090..73037
                     /locus_tag="IXT21_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4870467.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            73167..73907
                     /locus_tag="IXT21_003150"
     CDS             73167..73907
                     /locus_tag="IXT21_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870468.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(74198..74866)
                     /locus_tag="IXT21_003151"
     CDS             complement(74198..74866)
                     /locus_tag="IXT21_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4870469.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            75000..75287
                     /locus_tag="IXT21_003152"
     CDS             75000..75287
                     /locus_tag="IXT21_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870470.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            75703..77241
                     /locus_tag="IXT21_003153"
     CDS             75703..77241
                     /locus_tag="IXT21_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ4870471.1"
                     /translation="MNSFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            77238..78203
                     /locus_tag="IXT21_003154"
     CDS             77238..78203
                     /locus_tag="IXT21_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4870472.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            78190..79512
                     /locus_tag="IXT21_003155"
     CDS             78190..79512
                     /locus_tag="IXT21_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4870473.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      79575..79679
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            79753..80496
                     /locus_tag="IXT21_003156"
     CDS             79753..80496
                     /locus_tag="IXT21_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4870474.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            80511..81503
                     /locus_tag="IXT21_003157"
     CDS             80511..81503
                     /locus_tag="IXT21_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4870475.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            81515..82252
                     /locus_tag="IXT21_003158"
     CDS             81515..82252
                     /locus_tag="IXT21_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4870476.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            82242..82964
                     /locus_tag="IXT21_003159"
     CDS             82242..82964
                     /locus_tag="IXT21_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ4870477.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT        28559 a        14136 c        17575 g        22809 t
ORIGIN      
        1 tattctttag gatattaaaa ttaactagca ccacgggtga aagattatat tgcctatgta
       61 aaaaatcatg gcggagaaaa cacagtagaa ttagcagaag cgtattctaa agatgtgctg
      121 agcccacttt taggaaatga ggaagggaaa caataccgat attggcggat ccagtccttg
      181 ctttacaatg gcggctatct ctatcataat ggaggtaaca tgttttatcc tgatattgtc
      241 aaaatgaatg aaaaaataat agagacctat gaaaaagtct ttaagtaaaa tagcaggaaa
      301 tgttcaatca tgaataaaat taaatgaaag aaggagtgcg caaatagtgg aaaaaaggca
      361 aaaaatggaa aatcaagaag tggggtacac aaccaaagac tggatgttac ggtttgtgaa
      421 gggaatgttt atcggttcag gatttatctt gccaggagtc agcggtggtg cattagcagc
      481 tgtctttggc atgtatgagc ggctgatttc ttttttagcc caccttacaa aaaatttcaa
      541 agaaaatgtt ttgttttttc tgcctgttgg tctaggcgga gtgacaggca tctttctact
      601 ttctttttta gtcagttttt tattaggtgc gtatgaaaca acgatattat ggttttttgt
      661 cggatgtatc gctggaactg ttcctgcgtt atggaaagaa gcagggaaaa aaggaagaga
      721 tacttctgat gtgatcatca tggttgttac ttttgttctg gcttatttgt ttttattata
      781 cggcgctcaa ttatttgacg ggcaagtcga tcagaatttt ggaacatggc tgattgccgg
      841 cggattgatt ggtttgggta tgatcgtccc aggattgagc ccttctaatt ttttggtgta
      901 tatgggattg tataaagcga tggctgacgg atttaaagaa gtacgacttg aagtgattgt
      961 gccgatcgga attggtggga ttctttgtgt tcttggattg tcaaaaatca tggattacat
     1021 tttctcaaag gcatatagca agctattcca tttcatttta ggtgtagttt ttgcttcaac
     1081 gattatgatc attcctaccg attacgcggg attgggagtt gctggggtac tagcttgtcc
     1141 tgttcttttt atagccggta cagcactagg ttggtggatg agccgtttgg aggaacaata
     1201 taagtaaaga tagattgata ataaaaacag cacacgggtg attcaaacaa aaaggttggc
     1261 atcttttttg taaaatcatt cgtgtgtttt tctataaatt ttataaaaac aagttgtttt
     1321 taatcttgga acacgctaga cttaaacacg tgattatttt tgaaagttgg cggaaaatgg
     1381 agaaaaagaa aatgaatgga cttacacagc aagaggtagt tcagaaaatg gcttctggcc
     1441 agcaaaatga ctaccaggaa gatgcatcaa aaagtacaaa acaaatattc agcgataata
     1501 tattgacgtt attcaatttt ctgaactttg gtattgggat ttgtctgtta tttgtaggtg
     1561 cgtattccaa catggcgtat ttagcaatta ttgtcgtcaa tatcgttatt ggaatttatc
     1621 aagaaataca tgcaagaaat cttgtacgga aattatcgat tgtttcccaa tcagaagtac
     1681 tagtgatccg ggacggtaaa gaacaatcga tttctactaa agagctggta ttaggagatt
     1741 tggtaaaaat cggagcagga gaacagatac cagccgattt acaggttata tctggacggg
     1801 cagaagcaaa tgaagctctt ttgacaggcg aatctgattt gattgtcaaa gaaactggag
     1861 ctgaattgct ttctggaagc tttttgacaa gcggttctgt catggctgaa gttatccgag
     1921 taggtgcaga taattatgct gtccgcttga ctagcgaggc aaagactcac aaagctattc
     1981 gttctgaact tgtagattcg attcgcaaag tatccaaatt cactagtttc gtgattattc
     2041 ctttggggat catactattt ttggaagcac tgttcatccg cgatgcttct atcaaaattt
     2101 ctgttattgc atctgcagca gctttgttag gcatgcttcc aaagggtttg gtattgctaa
     2161 taagtattgc attaacgaca ggggtaacca aactggcaaa aaaacgaatc cttgttcaag
     2221 acatgtattc aattgaaacg ctggcccatg tcgatacatt atgcttggat aaaacaggga
     2281 ccattactga agggaaaatg agtatccagc gggtagaagc actacatccg ctgtacgaga
     2341 aagccatccc agaaattatg gggacgtatc tggcagaaag cagtgataac aacatcacta
     2401 tgcgggctat gagagattat tttgagcttt ctaatcggta tgatgtgaca aacaaagtcg
     2461 cattttcttc tgatcgcaaa tggggcgcga tggaatttcc agaactggga gtcgtgtacg
     2521 taggagcgcc tgaacgtttg gtggaagaag atcggcttcc ggacagtcta aaaacagccc
     2581 aagcgaatgg ctatcgcgta cttatgttgg ggatcgctcc aaatcagcaa ttgaacgaag
     2641 aaaaaccagc atttgttgag ccggttgctc tgtttgaaat cgatgatcca atccgaaaaa
     2701 acgctcaaga aacactagct tatcttcatg aggaaggtgt ggatctgaaa gtgatttctg
     2761 gagacaatcc ttttactgtc tccaatatcg ctagacgtgc aggattaccg aattatgatg
     2821 cgtatgtcga tctttctcaa attactgaag aaatggaagt gcgagaagct gctcatcgct
     2881 atacggtttt tggacgagtg agtccgcagc aaaagaaatt gctggtaaat gaattgaaag
     2941 aaagcggcag aacggttgca atgacaggcg acggtgtgaa tgatgttttg gcgttaagag
     3001 aagcagattg cagcattgct atggcagaag gtgatggagc aacacgtcag attgcgaatc
     3061 tggtattgtt ggactctgat tttactacat tgccagaagt cctgtttgaa ggacgccgag
     3121 tggtcaacaa tgtgacgaaa gtctcaggca tcttttttat caaaacgatt tattcattta
     3181 ttttatcgat tatatgtgcc gttacagcaa ttggatttcc gtttatccct atccaaatca
     3241 cgctgattga tttagcgatt gaagggtatc cttccttctt tttatctttt gaacaagata
     3301 aaagaaaaat cacttaccgt tatttgccta cagcacttat taatgcttta ccaaatgcct
     3361 tgcttgtagt agtgaatatc attgcggtgt atctacttgg aaaaacattc gcatggggaa
     3421 atgctgaaac tactactttg atgtattact tgctgattgg gatcagttgc atggcagttg
     3481 taaaagcttg ttatcccttc aacccattgc gcatcttcct agcagtaacg acgatcgtag
     3541 ggatatatgt agctgcaatg ctgttccatg gaattttgga agtaaactta gggttgggag
     3601 agagcgtagg atacttcttt gtattcatgc tcgtaaatat tgttcttcga gtattgtact
     3661 ggaaggctga tattcaaaat cagttactga aaagagtagc aaattgatgc ttaatgagat
     3721 agaaatgtgt tatacttgaa aaagcaatga aatatgatac aatcaaacat gtataattgt
     3781 gtcctttaaa atcgcactga tttcagttag tgcgattttt taccggttat tctcctgaaa
     3841 tatcttctgt taaacccaaa agatatcaat agagcagggg gtgaaaaaat ggctcaacgg
     3901 attcctcaag aggttatcga aacagtacgc agtcaaacga atatcgttga ggtgattgga
     3961 caatatgtcc agttgaaaaa gtctggtaaa aattacttgg ggctgtgtcc ttttcatgaa
     4021 gaacgtacac cgtctttttc tgttgcagaa gacaagcaga tttttcattg ctttggttgt
     4081 ggtaaaggcg gaaacgtgtt tacttttctt caagaactag aaggtctaag ttttccagaa
     4141 gcagtggtaa aagtggcaga cttcgaacag ataccgttag aagatcaatg gcgtcagcgt
     4201 actatagctg tccaaaatcc tgaatctgct acggggaaac tgattgctgc tcatgaaaaa
     4261 gcagcggaaa tctatcatca catgctattg catacaaaag ctggacagcc agctttagaa
     4321 tatttgcaaa gtcgtgggtt gtctttagaa ttaattgaag aattcaacat aggttttgca
     4381 cctaatgagc gttcttttct tcagcaagta ttcaagaatg aatcaatttc aaatgaattg
     4441 ttagaacgct ctggattgtt tgtcacgcat gatgatggcc gtatgtctga tcgtttttat
     4501 caaagagtga tgtttccact tcgtaattct caaggccaga cgatcggttt ttcaggacgc
     4561 tggcttgcac ctgaaaaagg gcaggaaaaa gatcagccca aatacttgaa tagtccagaa
     4621 acagaactgt tcaataaaag acaagtttta tttaatcttg ataaagcaag aagcgatatc
     4681 cggaaaaacg gagaaatttt gttgttcgaa gggtttatgg atgtgattgc tgcatggcag
     4741 gctggtatca aaactggatt agcttctatg gggacaagtc taacagctca acagatccaa
     4801 caaatggaaa agctgacgaa agaattggtc ttttgctatg acggagacaa cgcaggtttt
     4861 gaagcaacca atcgagggat tgaattgctt cggcaaaaca gtcgattagc actttctgtc
     4921 gtagtcgttc cagaaaagct tgatcctgat gaatatctcc gtaagtatgg agaagcatct
     4981 ttccaagaat tagtggctca tgggcgcgaa acagtcttta cttttaaatc acgttattta
     5041 aaacaaggga aaaatttaga taatgaaaaa gacaagatcg aatacctcga agaattacta
     5101 gttgaactta gccgagttgt ttcaccaatc gaacaagata tgtatctatc tcaactgtca
     5161 acagagtttc agatcggcag agagacgata caaaaacaac tgcgcgatct tcgaaggaca
     5221 aatcagcaaa atcgtcaaga acctcaggaa agtcaacaca ctcaggttac gagacaagaa
     5281 actgcacgga aaaagagacc tttgacgcaa gtagaaaagg cagagcagat tctattatat
     5341 cgtgcattta aagaagtttc tgtccgtaat attttaaaag aacgagaatt tcagtttatt
     5401 catgatgcat atgcagaagt ttatttttta ttcgatgcat atgtctcaca acattatgaa
     5461 ttcaaccttg cagagttttt agactttttg caagatgaaa atttaaggcg gttggtggtg
     5521 gaaatcgaat atttaccggt ggcagaagaa agttcgccac gagaaatcca agacttgcta
     5581 gatgtgatca gcaaatctgg cttagctgat gaaataacat taaaaaaaca aatgcagcaa
     5641 gaagccagac gaactggaaa caaacagctt gagcttgaat tgacgattga aatcatcaat
     5701 ttaacgaaac agttaaaaca agccaaatag ctactcaagg ggggccttct tttatggcaa
     5761 atggaacaga taaaaaaacg tatgaagcag cagtagcagc tttcatcaaa gaaaacaaac
     5821 ccaaacgaca agttgtatat gatgatttat ccaatcagct tgctacacca ttcactttga
     5881 atgcagatga aatggacaaa ttgatccaaa agattgaaga cgcaggtatc agtgtggtgg
     5941 atgaaaatgg tgatccatca attcacagct tgaagagtgc tgagaaaaaa gctgaacaag
     6001 caaaaacaga agatctttct gcgccaaccg gtgtaaaaat caatgacccg gttcgtatgt
     6061 atctgaaaga aattggccgt gtctcccttt taactgcgga agaagaagta gcgttagcat
     6121 tgaaaatcga agaaggcgac caagaagcga aacagcgtct ggcagaagca aaccttcgtt
     6181 tagtagtaag tatcgctaaa cgttatgtag gacgaggcat gcagttcctt gacttgatcc
     6241 aagaaggaaa catgggactg atgaaggcag ttgaaaaatt tgactaccgc aaaggattta
     6301 agttttcaac ctatgctaca tggtggattc gtcaagccat cactcgagca atcgctgacc
     6361 aagcacgtac gatccgtatt ccagttcata tggttgaaac aattaataaa ttgatacgta
     6421 tccaacgtca attgctgcaa gaccttggaa gagaacctac gccagaagaa atcggtgcgg
     6481 aaatggatct tccaacagaa aaagtccgag aaatcttgaa aatcgcgcaa gaacctgttt
     6541 cccttgaaac gccaatcggt gaagaagatg actctcactt aggtgatttc atagaagacc
     6601 aggaagcaac aagtccagca gaacacgcag cttatgagtt actaaaagaa caattggaag
     6661 atgtactaga tacattgact gatcgggaag agaatgtcct tcgcttacgt tttggtttag
     6721 atgatggccg gacgagaaca ttggaagaag tagggaaagt atttggtgtt actcgggaac
     6781 gtattcgtca gatcgaggct aaagcattaa gaaaattgcg ccatccttct cgttcaaaac
     6841 aattaaaaga tttcttagaa taagcgtgga ctattgacca gaagagaaat cagtaaaact
     6901 gtttggttta ttttaaaaga aaaaaagctt gagactacgc agtctcaagc ttttttttct
     6961 ataggaagga aattcattca ttatttttaa gtaaatccta aaaataagct attggcgtga
     7021 cgtatagaat atgatagatt aaatggtagc gtaaacctag taaaggagcc gatcgctaag
     7081 tgatgaaaaa atgtccaaat tgcggatcga aaaaaatgga gcagaatact atctgtccag
     7141 actgcggtca tcaaataact gcagaagatc agcaaataaa agaatcaatt gaagaaacaa
     7201 atacagatgc agctatgcag gataaatcaa ctgaagatgc agtctttcct gatacagaat
     7261 taaacgatcc aatcgaatgg tcggaattga aagatttacc tctagaatct gtaatggaat
     7321 tattcgaaaa agcagaacca gaagacgaag aaccacctgc tcacgatcaa gtaactaata
     7381 tagagaaaaa tgaacagaag aaagcaactg ttcatgagac ttcggaagaa tcaggaaaga
     7441 atcaagaaga aacgcttgaa tcaaaacaaa aaagaagagt agctgaactg aaagaaatcg
     7501 tcgataatga ggaagaaaat tcgatactat ccgcttatat caaagcacat cgtgaagata
     7561 caaaagaaga gcacgcgaaa gagctgctca aaatgatcag tgaaaaaatt gcacaggaaa
     7621 acaaagtaga gccagaaaag gaacaagaaa ctgaaaaaga ctctgataga gaaaacgcca
     7681 caggtaaaga aacagtaaaa ataaaagacg taaaaacgga tatcacagaa aaaactgaag
     7741 tatttactga aaaacaatta gaaaaggaaa aaacgaataa actgccggaa gcagagaaaa
     7801 aagaaatact cgaaaacaca gacaaaaagt caacagagcc cgatgaaata aatagtgaat
     7861 ccgatgctga aaatcaatct gtaagcaaag agggtactga tcttgaagca aaaatagaaa
     7921 aagaagagaa ccagcctaag ctgtttgaag cagaagcgaa atcaataaca atcgtgccag
     7981 atactgaaaa aacacatcaa gaatcaaact tagaaaaaac agatccagat aaagcgcaaa
     8041 agaaaaaatc aaaaaaagta ccttatctcg ttttagcagc agtacttcta ctcggtggag
     8101 gaagctgggc ttattatgac catcatcaaa aagtagaagc cgaagtagca gcagaagcaa
     8161 aaagacagca acaaatggaa gaacttcatg agaaacttgc tgccttttat acagatgatc
     8221 accatcaatt tattcgtgca gatatgattc atcaagattt atcaaaacta aaaaatgatt
     8281 tatccagtct taagaatgaa aaaggatatt cagagttaga aacaacttac caagatatcc
     8341 aatctaagat ccgagatatc caaaaagtga atgagtggtt catagcacct gccatcgtcg
     8401 atgatcatct agcagatgac atcaagttga aagcagataa agagatccaa gcaatcgaaa
     8461 cggataacac agattttgga aaattgattc aacaagcaca aacagaagct aaaaatcaat
     8521 atgctgaatt gcaaacagct aaagaaaaaa cagctatagt ctttaaagac ggaaaagtag
     8581 tggattcagc aacacgcgac caatacaaga cggcaaaaga agcagtagaa aaagtgaaaa
     8641 atacagatct atctgctcag ttgaatgaac aattgaaaca agtagataag atgctcactg
     8701 ataaagagaa aaaagaagaa gaggaaaaga aaaaagcaga agaagcgaaa aaagcagctg
     8761 aagaacaagc aaaacaagcc gctcaagcag cagctgcagc tcaagccgct caacaagctg
     8821 cggtcgcaca agcagcacag caagaagcgg catcctcgac cccttcctct tcaaactcag
     8881 cgaatcagcc aatcaaatct gccagagcaa gtgatattgc cgatgcatct aatccagcat
     8941 ggacgtgggc accaggtgtc caaagcaaag tgattgctac atgtatccaa cgtggatata
     9001 tcgttgaagg aggttacaga ttagaaaaag ccagaatcga aaatggggaa ggctattata
     9061 atctatatgc cacttcaaca aaatctgcat tgatgaaagg cattggagaa agtgctttgc
     9121 cattttacat cgtaaccatc aactgtaaaa ctggatggtt cggaggaaat gggagcaatt
     9181 aattttaggc aaatgaaaga ggctgtgagc ctgccgttta acaccgttta ttcgaaaaag
     9241 gggagtatga cacaactgtt gtcatactcc ttttttcacc tttccaaacc aaggctcagc
     9301 tattcaagta gataacatta tgttatgata gcaaagaaat gtattaagaa aacctttcac
     9361 tatagaggga gaaaaaaatg aacgatttat tagcaactat atttacaggt attattattg
     9421 atgaaaatga aaaaaactac tttgtccaaa aaaacggaca aacctttggc ttaagcaaat
     9481 cagaaggtga acacaaaatc ggtgaagcag ttgaaggatt cggttaccaa aatcaaaaac
     9541 atgaaaatcg gatcactaca caaattccta aaagccgtat cggtcattac gcttttggaa
     9601 tcgttactgc tacacgaaaa gatttaggtg cgtttgtcga tatcggtctt ccagataaag
     9661 actttgtcgt atctcttgat gaattgccga caatgacaga gctatggcct aaaaaagggg
     9721 atcgcttgat gatttccctt agagttgatg caaaagaccg tatttggggc agtttagcag
     9781 atgaaaaaat attcaaatct ttaagcaaat atggaacaga agaaatgaaa aacaaagatg
     9841 tgataggaac agcataccgc ttgaaattaa caggtacgta cgttttaacg gaagatttct
     9901 atcttgggtt tatccatcct tctgaacgat ataatgaacc tcgtctaggc gagcagataa
     9961 aaggtcgagt gattggtgtc cgaccagacg gtgtactgaa tctttcattg aagccaagag
    10021 catatgaagc tatatcagat gatgcacaaa tgattttaac cattttagaa cgttctgcag
    10081 atcagcaaat caactttaca gataaatcgg atccagaaga aatcatgaaa gcttttggta
    10141 tcagcaaagg agcatttaag cgcgcattag gtaatctttt gaaacaagga ttgattacac
    10201 aagaaaacgg tgtgacaaaa ctagtcaaaa aatctaattg agaatccctt gcatgttttt
    10261 cttaactagc ttataataat tttaattaac atagtgttat atcttttttt atttgtaatt
    10321 attataaatt gttggggttg agtaaatggt catgaatgct atttcggcga tgaaaaaaac
    10381 aaaacagcag ctacatgaag cggggtttaa gctgacaccg caaagagaag ctactctgct
    10441 tgtgcttttg gaaaatgaga aagagcattt atctgcagaa gaaatcttct ttttagtgaa
    10501 aaaaaagagt ccggaaattg gacttgctac tgtttatcga acccttgaaa tcttgacaga
    10561 attgaaaatt gtcgataaag taagttttaa tgatggatta gcccgttatg atttgagaaa
    10621 agaaggcgcc aaacatttcc atcatcattt actatgcttg aactgcggca cgatcgagga
    10681 agtagaagag gatcttttac ttgaagtgga gcaagtagta gaagaacgtt atcatttttt
    10741 agtaaaagat catcggctaa cgttccatgg aatctgtcag gaatgctatg aaaaagaaaa
    10801 aagaaaccag tccgtctgag tgtctagacg gctttttctt tgctcagatt atggaaaaag
    10861 actatcagaa tgaagcggta tctgatatga tagagaggca tgagggggat tattatgaaa
    10921 gagcaaatca acgattatct ccactacttg aatattgaac ggggattgtc cgccaataca
    10981 cgacaaagct atgaacgtga tttggaacag tacttgcatt ttctacagga gcagaaaatt
    11041 gcttcatggc aggatgtcga tcgcttttta gtcatccgtt ttctggagaa gttgcatgaa
    11101 gaaaaaaaag cgacagcaac tgttactcgg atgatctcaa gtctcagaag attccatcag
    11161 tttttacggc aagagcgtat cactgatcat gatccgatgc aacatatcga tacgccgaag
    11221 aaagcacaga aattaccaag tacattgact ctgacagaag tagaaaagct gattgaagca
    11281 cctgatacga caaaaacgtt agggataaga gatcgtgcaa tcttggaagt catgtatgct
    11341 actgggatgc gcgtcagtga attgattgga ttgaaattgg gagatcttca tctttctctt
    11401 ggactggtac agacagttgg aaaaggagat aaagagcgga tcattccttt aggtgattat
    11461 gcgatccaat ggctggaacg ctacttagac gaagcacgtc cattacttgt aaaggacgca
    11521 tctgaaatgc atgtatttgt caataatcat gggaaaggtc tttctcggca aggaatctgg
    11581 aaaaatctga agcagttagt acgagaggct gggatttaca aagaagtaac accacatacg
    11641 ctgcggcata gctttgccac acatctgcta gaaaatggag ctgatttgcg gacagtacag
    11701 gaattgttag gtcacgcgga tatctctacg actcagattt atacacatat cacgaagaaa
    11761 agaatgacgg atgtttataa gcagcatttt ccaagggctt agtcatagga aggtgagaag
    11821 atgttaacac attaccggaa agaaaaccgt aaaattgcta tgggactatt gagtttccat
    11881 aagaaactga caggtaaaac ttctttgttg aaggagattg atacgtacga atctgaagac
    11941 gattatgaac tttttttctt tacgccagaa ggttcaacca acattcaagg gattattggt
    12001 atttactggg caacgaacga tcagctgatc atccatgaca tttctttgaa tccgtcttat
    12061 cgcggtgaga atatgggctt tcaaatgtta gatgaattac aggaaaagta cccggatgtc
    12121 tccatccgtg caactgaaat cacagagcct tacattggaa aatggctagc aaagaaaaag
    12181 aagaaagagt gaatgagttg acggagatca atattaaatt agatgtattc gagggtcctc
    12241 tcgatctgct gcttcatctg attcagcaaa tggaaatcga tatatatgat ataccgatcg
    12301 ctacgatcac ggagcaatat atgaattata tccatacaat gaaaacactc gagctagctg
    12361 ttgcaggaga atacttagtg atggctgcta cattgatggc aatcaaaagc aagacattgc
    12421 ttcctgtcca agaattcgtc acagacgatg aagaattatg ggaagaagat cctcgagaag
    12481 aattggtcac tcagctttta gaataccgta aatataaata tgcagctgaa agactgacag
    12541 aaaaagcaga ggaacgaagt ctttattaca caaaagaacc tatgaacatc gatgggttag
    12601 cggatgctga aaagccgttg aagcgaggac aagtcaaaaa aatcgatttg atccgtgcca
    12661 tgcagaaaat cttagaacgt aagaaatttt ctcaaaaagt ggaaaaaacg atcacgcccg
    12721 atgacacaac gatcgaagaa cgaatggtat ttatcgaaga gcgtataaaa agaaatgcgc
    12781 atggctgtac tttcgattct ttttttgagt caccttctaa aagtgaagtt gtcactactt
    12841 ttatggcttt gctagaattg atgaaaaaag ggcagatcat tgcgcaacaa aaagaaaact
    12901 atgaatcaat tatgctttat ccagcagtag gagataccga acatgacgaa attaagtgaa
    12961 cttgaagccc ttctttttat tgttggcgag gaagggatca gtaatgaaga attaagcgga
    13021 ctgttaaatc tcagcgaagc agaagtcaca agtttagtga aagaattaaa agaatcttac
    13081 actcaaaact cgcactcagc tttgcatatt tttgaaacgg aaagccatta tgtattaacg
    13141 actaaaaaag agctggctcc cttacttaag cgatatgcgc aagggacatc aaacacatta
    13201 tctcaagccg ccgtcgaaac cttagcaatc atcgcttata aacagccaat cacaagagct
    13261 gaaatcgagc agattcgagg agttcaggtt tcaggagcgg tacagcgtct agctgcccat
    13321 aaactgattg aagaaaaagg tagagtagaa ggaccaggtc ggccgatatt atatggaaca
    13381 actggctact tcttagatta ttttggtttg aaatcattag aagaactgcc tgatacacaa
    13441 gaaatggaaa tttcacttga agaagacgtg tcaccagatc tattctttga tggaatgaat
    13501 aaaggagaat aattgtaatg gagagattgc agaaagtaat tgcccacgct ggcatcactt
    13561 ctcgtcgaaa agcagaagaa ttcatccttg caggacgtgt aaaagtaaac ggacaagttg
    13621 taagggaatt agggataaaa gttgggaaac atgacactgt ggaagtggat gaagtcccaa
    13681 tttatcaaga agaatacggt tactatttat tgtacaaacc taaaggagtt atttctgctg
    13741 ttgcagatga caaaggaaga aaagttgtta cggatctttt gccgatggta aaagaacgta
    13801 tctatcctgt aggtcgactg gattacgata cttcggggat cttgctactg acaaatgatg
    13861 gagattttgc tcagcgcctg acacatccaa aacatgaagt ggataaagtt tacgttgcaa
    13921 aggtaaaagg gatcgctaca aaatcgatgc ttgcaccttt gacaaaagga ataaagatcg
    13981 aaggacgaaa aacatctcca gctaaattcc aaattttatc agtggatcct aagacaaacc
    14041 acagtatcgt ggaattgacg atccacgaag gacgtaatca tcaagtgaaa aatatgcttc
    14101 aagcagttgg tcttcctgtg caaaaactaa aacgtgaaaa atacggagat ctgactttac
    14161 aaggcctacg tcctggggat taccgtgaac tgaacaaaaa agaagtcagc agtttgctga
    14221 accactcaaa atgaacactt actttttata agtgttagat gttgcgtttt ttgaatagat
    14281 atgtataatg aatatgtaaa caaaaattat ataaggttcg tcttcagggg cagggtgaaa
    14341 ttcccgaccg gtggttatag tccacgacct attttgcaaa aaaatagctg aatcggtgaa
    14401 attccgatac cgacagtata gtctggataa agaagataga gcttatttga cgacgcttga
    14461 tcaaataact ctaactctat tttccctgga aaatagagtt ttttcatttg gagagaaacg
    14521 tcaaaaagtt cgctgaagag gagtcctaac aggaggattt caaatgaaga acacccgtgt
    14581 acagaaaatg gttgcagtag cgatgtttgc tgcgatcgca ttagttttac agtattttgc
    14641 ttttccgatc atgccggctt ttagtttttt gaagatcgat tttagtgata tctcggtatt
    14701 gatcagcatg ttcttattcg gtccagcggc cggagttgca acagcgttct tgcgttcagt
    14761 attacatctg ctgactacag gtttttctcc ggataatttg gtaggagaca ctgctagttt
    14821 cttatcaaca acgatcttta cattcccgat ttattatttc tttagtgtaa agaaacagca
    14881 taaaggaaga aacaaatttt taggtgtcat tacaggaacc atcgctctaa ctatttttat
    14941 gagtatcgca aattatttcg ttattacgcc tctttactta atgtttttag ggctgaacgc
    15001 aacacagatg ctgggcatgt ctttagcaaa ctatgtactg attgggatcg ttcctttcaa
    15061 cttgatcaaa ggattcatcg tgagtgctgt ctttttagtg ctgcatgcgc aacttcttcc
    15121 atggttaagt cgaaaacaac atcagttcga acaacgtcat acagcagtca aataaatatc
    15181 aaaaagatca agaaatttct tggtcttttt ttttggaaaa aaatacaaga catgcaataa
    15241 tgtaaacgtt ttatttgttt gttttgagta tttttttgat ttatgcataa aaatcaatca
    15301 attctttgca aatgtgaaat tcttctcctt ttaaaagacg aaaatgtttg tttataaaaa
    15361 aaaggaaacg ctacaatgaa aaagaagaaa gctttaaaaa cctgaaaaaa gaggagggaa
    15421 tagggaaaaa cgaacttttt gaacaaaagt tttacaaaat gcgtgttatt atttttgttc
    15481 tgatactctt tttgataaaa taacattaga gccaaaattg ttgtctctaa cggaaaagaa
    15541 aggaagaaga agatggaaca aacaaaaaag aaaaaatttc aaatgccttc agcgtatacg
    15601 gtcttattta ttattattgc agtaatcgct gtacttactt ggtttatacc agctgggcac
    15661 tatagtgtag acgatgcagg caacatcgtc gctggatcat atgaacgagt acaacaaaat
    15721 cctcaagggc tgtgggatat tttcatggcg ccaatcaatg ggatgctagg tgtaccagcg
    15781 ggggagctaa ctaaagaaac acctgctgca atcccaatct ctttcttcat tttggttgtt
    15841 ggtggatttt taggtattat caacaaaaca ggggcactag atgccgggat tgcttcagtt
    15901 gtgaagaaat tcaaaggaaa agaaaaaatg cttattccag tattgatgct gttatttgca
    15961 ttaggcggct ctacttacgg aatggcagaa gaaacgattc cattctatgc tttactgatc
    16021 ccagtcatga tggctgtagg ctttgataca gtagtagctg tagcaatcgt attagtaggg
    16081 tctcaagtag gctgtctagc ttcaacggta aatccttttg caacaggcgt agcttcacaa
    16141 gctgttggga tttcgatggg tgaaggtatt ggtcttcgat tcttgatgtt cgttattttg
    16201 ttagctattt ctattgtata tgtgtatcgt tatgcatcaa agatcgaaaa agatccaaca
    16261 aaatcattag tatacaacca acgtaaagaa gatatgaagc atttcgatat tgaagcaatc
    16321 ggtcgtcaag aagtaatcac gaaaaaacaa aaacatgtta atgtattgtt ctttattact
    16381 tttggtatca tgatcatcag tttgattcct tggacaacct tgaatgcgaa tttcacgatt
    16441 tttgaatcat tgacaaactg gttaacaaac cttccggtac taggtacggt tctaggtaaa
    16501 agcatgcttc cattaggtga ctggtacttc ccagaaatta ctatgttatt cttagtaatg
    16561 tccatcgtag tagcaatcac ttatggcatg aaagagtctg aatttgtctc tgaattttta
    16621 gctggtgctt ctgatttgat cggtgtagca atcgtagtag ctgttgctcg tggtatccaa
    16681 gtggttatga acaatggatt aatcacagac accatcttac attggggcga acaaggatta
    16741 agttcattat cttcaagtgt attcatcatt attacattta ttttctatat ccctatgtca
    16801 ttcttgattc cttcaacttc cggcttagct gctgcaacaa tgggaatcat gggacctatg
    16861 ggtgaatttg ccggcgtcgg taaggattta gtaatcactg cttatcaatc tgcatctggt
    16921 ttagtaaact taattacacc aacatctgct atcgtaatgg gtgctgtagc aattgctcgt
    16981 ttcgatgttt cagtgtggtg gaaatttacc ggtaaattaa ttgcgatatt atttgtagcc
    17041 atctgtgtga tcctaggtgt agcagcaatg ttctaaacct agacaaatac aggtacaagg
    17101 tctatcataa gaatgaagaa accgataaac ggctatcgat aagcacgtgg ataagtcaat
    17161 agctccattc agtgagtgtt ttttctttca tcatttgtta agaacattct gcttgctgat
    17221 ggagcaggct taaaacgtca ttatttgaga agaagaggtt gtgtcggagg aatttgacat
    17281 gacccttttt aacatattag gaggataata tcatgaaaaa atttgtgaca gaacaacatc
    17341 atgaacaagc attagaatca ttgagcgaat tgatccgaat tccttccgta ctagatgaag
    17401 cagattcagg acaaggacat cccttcggta aaaaagtgat tgaagctttg gataaagtat
    17461 tggaaatcag tgaaaagcta ggatttagaa catttaaaga tccagaaggc tattacggtt
    17521 attcagagat tggctctgga gatgaattat tcggtatctt gtgtcatatg gacgttgttc
    17581 cagcagggga cgaaaataat tgggaaacaa aaccattcga tccaactata aaagacggct
    17641 ggttagttgg tcgcggatcc caagatgaca aaggtccttc tattgcggca atgtatgccg
    17701 tcaaagcttt gattgatgct ggcgtggaat tcaagacacg tattcgtttt atctttggta
    17761 cagatgaaga aaatttatgg cgttgtttag aaaaatataa cgaaaaagaa gaaggaatta
    17821 cgcaaggatt tgcgccagat gctgaatttc ctttaattta tgcagaaaaa ggcttattgc
    17881 aagcttactt gacaggacca ggaacgaacg agttttctgt aaaagctgga ggagcgttga
    17941 atgttgttcc tgatgcagct ccttactctg gagaaaagct ttctgaagta aaagaagcat
    18001 tgaaaaaaca cgaattcgat ttcgaagaac aaggtgaagg catcgtcgtt cttggtaaaa
    18061 gtatccacgc gaaagatgca gcacaaggag tgaatgctat ttctcgtctg gcaattgctc
    18121 tttcagaagt atttgatttt ggcccaatca atttcttagg aaaattagtt caagaaaatg
    18181 caactggtga agctgtcgtg ggcaaaacag aagatgaaca atccggcgag ctaacgatga
    18241 actttgcaag tttggaaatc acaccagaac aaacaaaaat cggtgtagat atgagaatcc
    18301 ctgttacatt caagaaagat gatttagtag ctaaattaac taaaacagca gaaaaatatg
    18361 ggttgactta tgaagaattt gacttccttg attcattata cgtgccacta gacagtgagt
    18421 tagtgaaaaa tctattgggg acatatcgcg atattactgg agacatgact gaaccgtttg
    18481 tatcaggcgg cgctactttt gctcgtacga tgaaccagtg tgtggcattc ggtgcgatgt
    18541 tccctgatac tcctgacttt atgcaccagg cgaatgaacg atgggaatta agtagtatgt
    18601 ataaagcaat ggaaatttat gcagaagctg tgtatcgttt atgtgctaaa taaaaataaa
    18661 catacaaaaa gggcggatat tcaaatggat attccgtctt tttttatttg atgctggaaa
    18721 gtttgcattt ctctcttatt tttttaaggt ataatcatac ttgattaaga aaaaggggtc
    18781 actcagatag aaaaggtggg agaaagtgaa aatatttaga cgaatactaa ttggcttagt
    18841 catcatttta ggattactcg gtggagccgg ttattactat atacagcaaa atacgtacga
    18901 accatcgtca gaagcgcaag tgttggtaaa agaaggaaaa aacaaagaag attacttatt
    18961 ttttgaagga gagaaaaaga atcatggaaa gtctccatta cttatttttt atcctggtgc
    19021 tctcgtagag ccagaaagtt atagtatttg ggcgcaagat ttagcaaaaa acggcttttc
    19081 agtcgctatt gtcaaaatgc cacttgatct agcagtgatg ggcggaaaca aagcggataa
    19141 ggtcaaagaa gattttccaa attccgatta tgtgatcggt ggccattcat taggtggcgt
    19201 aatggcaagt cgttatgtca ataagaatca ttcagacaaa catttaaaag gggtattctt
    19261 catggcaagt tatcccgaca aaaaaggaac attgacagaa tcagctgttc ctgttttatc
    19321 gatcacggcg acaaaagata aagtattgaa tcaggaagct tatcaagaag ctaagcagta
    19381 tttaccggaa agaacagagt atgtatcaat caatggcgga aatcatggag gttttggcag
    19441 ctatggtgag caaaaaggag atggagaggc agaaatttct aacgcagacc agcaaactgt
    19501 gatcgtcaat acattggaaa actggctgaa aaagttgaaa taatataaat gggtcctata
    19561 gggtccgtga aaaaactagt caattaacta tagatagcaa atgaatcttt caaaataagt
    19621 cgggatttca cggaacaaga gaaacagatc ccggcttatt ttcaagttta gagaacttag
    19681 tgtacgtttt taaagtttcg tttatattta tattttattt tctaataaaa tcgctcgaac
    19741 tggacatttt tgatacgctt tttttacata tgtttcttct tccggcatca cgaactgttg
    19801 atgagcctct ggttcttggg caaataaaac gattccttct tcatcataat cgaaaatatc
    19861 tggcgcttcg atttgacaaa gaccgcatgc aatacagcgt tcaggtacaa gtttacatag
    19921 tagtgacatt tctaaccatc ctaacttaag gagaataata tgattgtttt tattttatcg
    19981 ctattttcat caggacacaa gctgcggatt agttctcttt accaactcct tgtgggaaaa
    20041 cgaacgactt cagtacttat ttatggattt acccacgaac ttttgtttat ccataacagt
    20101 tttccagaat tgaagcagga taagttttat caaattttgc aaaaaattgc gcagcaaggt
    20161 tggatcgaaa taaatgaaaa tgaagcaaag ctaacgcctg caggggctga tatgttgtcc
    20221 gaacatcaaa tagaacatac tggtttgcgt tttgaccgct atggcagaac aggggaaact
    20281 agttggcggc tcattaaatt tgctgttcaa gtaatttcta atctggcaac gggtattcaa
    20341 gactatcttc cagcagagac aagtcctttt tatactttcc aactaaaaaa atggcttagt
    20401 gaaagccaat tgccacgtga aattttgatt gatactgcgt atgagagttt ggtgcagctg
    20461 ttttcggaga taccagaaga agcagcagat tttttagcta accaattatc tggaaatgag
    20521 cgaacagggc tgcttcctta tcagctggca aaaaacaatg atgagtcagc ggtttatctg
    20581 caacaaagca gatgtatcca ccttttgtta gcacaaatcg aaaaacggcc agattcgctt
    20641 tggcacgctt tgatcgattc attattacag caaaatttca atcaaagcat gatgatcacc
    20701 aagcagatgt ttatgaatgg tcagacaatt gaccagatca tggcaatccg tcatttaaaa
    20761 agaggaacag taacagatca tcttattgag tgggccttgt tttttgatga ttttccatac
    20821 gagtggattc tctctgcaga aacaatcgaa cgtttggaac caaataagga ttccgtgcga
    20881 gagtggcgct ttagcgaatg gaatgtagat ggacaattag attatggaga atttcgtttg
    20941 tatcaaatct atctattgag aaaggaagcg atacagaatg ttaataaatg aattaaaaaa
    21001 atattttgga tttgattcat ttcgtcccgg ccaagaagaa attattcagg cgttgcttga
    21061 tggagaagat actctcgcta ttttgcctac aggaacggga aaaagccttt gctatcaatt
    21121 gacggggtat ttaatggaag gtctggtcat catcgtgtca ccgctgcttt ctctaatgga
    21181 agatcaagtg acgcaattac aaaaacgagg ggagaaacga gtagctgctt tcaatagttt
    21241 actttcgcaa tctgaaagaa gatacgtcct ccgtcatcta tcgcaatata aatttttgtt
    21301 tttgagtccc gaaatgttga ctaatccttc tcttttagag catctgaaaa aacaaaatat
    21361 tgcgttatat gtagtagatg aggctcattg cgtgtctcaa tggggagttg atttccgtcc
    21421 agaatatcaa cagttaggaa aaatccgaaa gcttttagga aacccggtca cactggcact
    21481 aacggcgaca gcaacagatt tagtagcaag agatatacgt catgtactat ttgatcgacc
    21541 accaaaagag atcagacaat cagtcaatcg gcaaaatatc tcgctttttg ttcggaaaac
    21601 gcagcaaaaa gaacaagaac ttgaacactt catggaaaga gcacaaggag cggcaatcat
    21661 ttattgtgca acaaaaaaag aagtcgaacg attgtatcat ttattcaggg aacgttttac
    21721 agttggatac tatcacggtg gtcttgatgc agctcaaaga agacaacttc aacagcagtt
    21781 tgtaaaaaat cagctgcagt ttctcattgc tacgaatgca tttggtatgg ggatcgataa
    21841 atctgatatc cgttatgtgg tccattatga tttgccagac agtctagaaa actatgtaca
    21901 agaaatcggt cgagcaggca gagatcagga agaaagtgca gcaattcttt tttatcaaag
    21961 tggggatgaa cgtatccatt atttctttaa tcaactttct agagaacagc gacaaagttt
    22021 tgagctttat ttggaatatg ccacagagca agctcccttt gatgagctgc aaaaaaaatg
    22081 gatggagctg atccagcagt ctgaaaaacc agaaaattgg gtggaacggc tcaaacgtca
    22141 ggaaaaagaa aaagaattcc gcctgcagca gatgcttcgc tatattaatg aagaaaactg
    22201 ccgcagaaaa ttcatcttag cttattttgg cgaaaaactc tccgaaaagc cgcagaattg
    22261 ttgtgatatt gatggagcaa aagcagaact tctgccaaag aggaagcttt tttctaagac
    22321 tgaacaactt cattgggaaa cgattttact aaatttattt aaaaaaaaca tggaacattg
    22381 attttttagg acaagacact ttttcctatg gtataataca cacgatagag tttaggaggt
    22441 aaaacagtga gcagaaaaga tagacatcaa tctacagaaa ataacgaaac acaagaacca
    22501 tgggaacaac caatctacga tacagatgat gaagtttcat caagaagttc tcagagacaa
    22561 caaaagaaag gaaatacatt atttctttcg ctatttttga ttttactggt cttatgtatc
    22621 gctattccag caggtgctta tttctggatc agaaatggat caagcaacaa tgcaacagca
    22681 gcaacttctt ctgaaacttc gacatcagta gtagaaagtt ctacaagtac aacaaaagag
    22741 gagtcaactg agacttcaac agtagaatct agtacaatcg cggaagaatc tactgtgtct
    22801 caagatacag gtgtaacgtc agaaagcagt attgcagcag agacaacacc gacttcctca
    22861 agtgaggcaa cttatcaagc aggggattca agcagcaatg cagcagaaac aacaccttct
    22921 tcatcagctg cagccggttc aacaacgact gttcaagcag gcgaaggtcc aaaacaagtg
    22981 gctgagcgtg cagggatcac cactgatcaa ttgtttgaat tgaatggctt agatccaaac
    23041 aactttatgc tttaccctgg tcaagaatta agaattaaat aataatagcc gcgctttgcg
    23101 gctttttttc tgagaaaaag gagttatatc atgacacaaa tcagtattgc aatcgacggg
    23161 cctgcttcat ctgggaaaag taccgttgcc aagatattag caaaagaatt gaattatatt
    23221 tatacagata ccggtgccat gtatcgcgca gtaacttatt tagctattac gaacaatgta
    23281 tctttttcag atgaagaagg gctagtttcg ctgatcaaaa agtatcctat ctctttttcg
    23341 caacaagaag atggacagca tgtattcata gcatcaaaag atgtgacttt agaaatccgt
    23401 caaccagatg tcacaaaagc tgtttctgaa gtctctgcac atggaaaagt cagagaagaa
    23461 ttagttgctg tccaacgaaa aataggggaa cgcggtggcg ttgtcatgga cggaagagac
    23521 atcgggacag ctgtattacc gaatgcagaa gtgaaaatct ttttagtagc aagcgtaagt
    23581 gagcgtgcag aaagacgttt caaagagaac caagaaaaag gaattccgac tgatcttgaa
    23641 acactgacga aagagataca ggaaagagac cattatgatt caacaaggga cgtctctccg
    23701 ctgaagcaag cagaggatgc tgttcgtatc gatacgactg gcaaatcgat tccagaagtc
    23761 gttgcagcaa taaaagctgt tgttttgaaa aaaggttatc ttctttttta aattaagaaa
    23821 aaaaatgaaa aacaattgtt ttttcaagga aaacgaacga aatcgcttta ttttttttga
    23881 ataatcaatt aaactaaaaa cagtaccgta cttacggcag aatgttggtt aggaggacaa
    23941 gtgttatgac agaatttgat caaaatcaag agaacaacca gtcaatggaa gacgcaatga
    24001 aaagcgtaca agaagtaaaa gttggcgatg ttgttaaggg cgaagtttta gtcattgaag
    24061 acaaacaagt gattgtcgga atcgaagggg ctggagttga aggagtcgtt ccggcaaaag
    24121 aattatcaac tttgccggta gaagatatca gcgaagtagt aaaagtcggt gatgtattag
    24181 atttagtcgt tatcagtacg atcggcaaag ataaagaaaa cggcagctat ctactttcaa
    24241 aacgccgttt agatgctaaa aaagtttggg aagatatcga aagagatttc caagagggca
    24301 aggtcatcga agcacctgtt acaaatgttg tcaaaggcgg tttagtcgtt gacgtcggtg
    24361 tacgtggttt tgtccctgca tcaatggtgg aagatcattt tgttgcagac ttttcagact
    24421 ataaaggcaa aacattgagc tttaaaatca tcgaaatcga accatcagaa aatcgtttga
    24481 tcttatcgca taaagcggta gtggaaaacg aaagagaaga aaagaaaaaa gaaattcttt
    24541 caactatcca tgagggagat gttctcagtg gacgtgttgc acgtcttact gattttggcg
    24601 catttgtcga cctaggcgga attgacggtc tagtccatgt ctcagagatt tcacatagtc
    24661 atgtagcaaa accaagtgat gtattggctg tcaatgatga tgtaaatgtc aaagtgttgt
    24721 ctatcaatcc tgaacaagaa cgtgtttcct tgtcaatcaa agacacacag cctggacctt
    24781 ggtcagaaat cgatacaaaa gctccagttg gcacagtatt agacggaaca gttaaacgtt
    24841 tgacaagctt cggtgccttt gttgaagtgt tccctggagt agaagggcta gtccatatct
    24901 ctcaaatttc acataaacat attgcgacac cacatgaagt gcttcatgaa ggtgacgaag
    24961 tgaaagtgaa agtattagag atccatccag aagaacatcg tattgcattg agtatcaaag
    25021 ctttagaaga aaaaccagct tcagaagaag aaccaaaaaa tgttgagtct tatgaattac
    25081 cagaagaaaa tactggattt actatgggag acattttagg tgatgcgttg aaagaagaag
    25141 tagaagaaaa agcaaccgat gattctgaaa aataattgat tttgtatctg tgactgagat
    25201 gaaagcagct gcttttgtct cagtttttct tttgaaaagc gattagtatt tcaataagga
    25261 ataaaaatac ttcgtttaat tgacaatcta tgcttgaaag ttggcaatgc attcgttaaa
    25321 ctaagtgaga ttggaaactt gtgaggagga aataatatgg caaatccaac aattgcaatc
    25381 gttggccgcc cgaacgtcgg taagtctacg atttttaacc gcatcgctgg tgagcgtatc
    25441 tcgattgtcg aagatactcc tggagtaact cgtgatcgta tttatgcaaa aggtgaatgg
    25501 ttaggtcgag aattcagcgt gatcgacaca ggcggaattg atcttggcga tgaacctttt
    25561 atggatcaga tcaagcatca agcagaaatc gcgattgaag aagcagatgt aatcatttgt
    25621 gtagtcagcg gacgcgaagg cgtgacagac gcagatgaga tggttgcgaa aattttatac
    25681 agaagtaata aaccagtgat tttagcagtc aataaagtag ataatccaga aatgagaaac
    25741 gatatctatg aattttattc cttagggcta ggcgatcctt atcctgtttc aggaagccat
    25801 ggactaggga ttggtgatat tttagatgaa gcagtgaagt atttctctga agagactgaa
    25861 gaagaggacg acgacacgat taagtttagt ttgattggtc gcccaaatgt tggtaaatcc
    25921 tctttgatca atgcgatttt aggagaagaa cgtgtaattg tttcggatat tgaaggaacg
    25981 acccgagacg caatcgacac ccacttcgtt tctgaaagtg gtcagaaatt cttaatgata
    26041 gatacagctg gtatgcgtaa acgcggaaaa gtatacgaaa atactgaaaa atacagcgtg
    26101 atgcgtgcaa tgcgtgccat tgaccgttct gatatcgttt tgatggtact gaatgctgaa
    26161 gaagggattc gggaacaaga caagcgagta gcgggttacg cccatgaagc tggtcgtgga
    26221 attattatcg tcgtgaataa atgggatctt gtaaagaaag aaaccaatac aatgcgtgat
    26281 ttcgaacaag agattcgtga tgaattccgg tatcttgatt acgctccaat cgtttttgtt
    26341 tctgcagtga ctaaacaacg gttggaacgt ctgcctgaaa tgatcgaaca agtaagtatg
    26401 aaccagaatc ttcgtatctc ttcggctgtg ttgaatgata tcattatgga tgctgtagca
    26461 atcaatccaa caccaacaga taaaggaaaa cgcctgaaga ttttttatgc tacacaagtc
    26521 gctgtcaaac cacctacatt tgtcgttttt gtcaatgaag aggaattgat gcatttttct
    26581 tatgaacgtt tcttagaaaa tcaaatcaga aaagcattta cttttgaagg gacgtccatc
    26641 cgaattatcc ctcgtaggag aaaataggca ttttaccata tcgacgtttt tttttcaaaa
    26701 gaaaaagaca gtttcgtttg ttacgtttct atgactaaaa aaagcatgtg aaaattggta
    26761 aaaaccttgc tattaagcgg ttcatatgat atcgtgataa cagaatattg aagttaatca
    26821 atatttcttt ttaaacttct ggaccactca gaaagtttta actaaatgat atttctatca
    26881 tatctcttgt ggaggaggtg aatctatcca tggcaaacaa agcagaatta atcgaaaaag
    26941 ttgcttcagc tactgattta actaaaaaag atgcaactgc agcagtagat gctgtatttt
    27001 caactattca agatgcttta gctaacggcg aaaaagttca attaatcggt tttggtaact
    27061 ttgaagttcg cgaacgcgca gctcgcaaag gtcgtaaccc acaaactggt gaagaaatcg
    27121 aaattccagc tagcaaagta cctgcattca aaccaggtaa agcattaaaa gacgctgtta
    27181 aataagacag ttgaaaaagc gcttgtggct ctagggctac aagtgctttt ctttttattt
    27241 cctagcagaa aaggaataaa taagagacag agattccgaa tccgtatgtc atacatagaa
    27301 aacacatgta ttttatttaa tttttattta tttgcaagtt atgaggtgag ctttgctctt
    27361 atcttttcta gaatttgctt actaatggta aaataaaaat aagaagagga gggatgcgta
    27421 tggaacttga ttatcaagta gaagatgcgt tgacgaaagt gttttacgga gaaacgctag
    27481 tcagtcagct aagagaactt tccattgatc agccaatctt atttttaacg aatcagcgct
    27541 attatgattt gtttgcagat aagatcaacc cgttatttgc aaatcaagca aacaatgact
    27601 ggtatatttg tgccaacact caatgcaatc atttaacaga attaaagaac ctgcttgatt
    27661 tcgtcaaacg ctatcccgaa aatcaatcca tgtttatcat cggatttgga aatgaaggga
    27721 tcatggatct ggcaggtttt tttcaaaaac atacaaccct tgattcatca ctatggatga
    27781 tccctgtatc gatccgttct ctaagccgag cattaacacc gcagcggacg attttgcagc
    27841 taccaaatcg tgtagtgctg caaaccatca atctgccaga acgcatcgtt tatgatcaaa
    27901 caatcagcga gcggcaggtg gacggaaagc agattgactt tttgactttt atctgttgcg
    27961 gaatgatttg tgatcatcgt tttttgcaaa atctgtataa aaactatcca aataaaaagc
    28021 aattaacgac tcggccgttt gccgggatga tcgaagaaat gatctatttt tatcaattag
    28081 aggcagaaaa cctgtcaaat tatggtaaac tgtttgaaca agctttttat ctgaccgaaa
    28141 atggccatct actttctgct agtatgaaaa aatttttagg aatgatgttt catttcgtgt
    28201 ggaatctcca agcatatcct tttgatttcc aactaaaaaa cttttttgtg tggctggact
    28261 tgcttggcta tccaatccac tggctggaac agatttcaaa gatggagtat ttggaaaaag
    28321 ttcttttttt ggcagaaaaa agcaaaaaaa tacttgtttt agaaaaaata ggtataatag
    28381 acggatacca acatccaaac gaaaaagaat tattgagaac aatgacggta tttcaacaaa
    28441 tcgtcagtga gataagaggg aattgaaatg aatacaaata gcgaaaaaat gttgcaagct
    28501 ttatcagatg aagacttgtc acaagcacaa atctttttag aaaaagcatt aaaagaagac
    28561 ccagcagata tattagctga actaggcgaa gaattgctgg ctattggctt tttgatcgaa
    28621 gcaaaacaga tttttgaaca actgatcaac caatacccag aaaatgacgg actgaacatc
    28681 ccgttagcag aaattgcaat cgaagataat gagatcgact tagcttttga atacctggaa
    28741 aaagtggata agcaaagtga ttactatccg caaagcttat tggcgatagc tgatttgtac
    28801 caagttatcg gaatccctga agtgagtgaa gcaaaattga aagaagcagc tgggttgctt
    28861 cctgatgaac cattgatcca gtttgcgcta gctgaattgt atttttcagt tgatcgtttc
    28921 aaagaggcag cagtggttta tgaaacattg ttggcaagcg ggataaatga aatttcagga
    28981 atctcgatgc aggaacgttt aggacaatcc ttaagtatgc aaggagaatt cgaagcagct
    29041 atcgtacctt tagaagaagc actgaaagaa gaaaggacag atgatcgact gttccagcta
    29101 gcttttacga atcttcaatt gaaagaaaat cagcaggcaa tcaactatct ggaagaactt
    29161 cgagaagtta atccgcatta tcaatcgtta tatctatatc taggacaagc tcttcaagaa
    29221 gaagaaatga ttgaagaagc acaaacagtg ttagaagaag gaatcaaaga aaatccttac
    29281 caagtcgaac tttatcacct ggcctcggaa aatgctttcc gtctgcatga tagacagcga
    29341 gcagaagaac ttttactgaa agctttagaa gtaggagaca aacaagatga aacattactt
    29401 actttaagta atctttactt ggatgatgaa agatatgaag atgtgataca agtgatcaat
    29461 cagatggaag aaactgctaa tccttatgca gaatggaacc tagcccatgc ttataatgaa
    29521 ttagaagact ttaccgttgc agctgtacac tatgaacagg cttatcatga actatcacat
    29581 gaaccagatt tcttgaaaga gtatgccttt ttcttaagag aagaagggca gctgaaaaga
    29641 gcgcaagaat tattgactta ttatttaaca cttgaaccag gagatatgga ggcactgtca
    29701 ttattagatg acctaacaga gaggtgagaa aaatgctaat tgatgtacgg gaaaagaaga
    29761 attttttaac gtggatggtc aatcatgtat cttttagccg aagagaagtt ttttggatct
    29821 tgaattatct tgccaatcat gaagcgattt taagcaatgt ccactttgtt gaaggggcga
    29881 attttgcgga tcgaggactg cagatcagtg atctatctgt tgaaggggaa cccatgaaat
    29941 tgtttttgca gggaaaagaa tttacggaca cagaccagat ttttcatgaa atccgtttga
    30001 attggaaaaa gcctttatat gtggaatgta tattcgaaaa tgcttggcag acaagagaat
    30061 atctatctat cttagaagac aatccatttt cttcttggga ttcttcagtc agtgaagaag
    30121 atactgaatt aattgaacga tattttcaag aaaaagaaga agaggcaaaa ctaaaaatgc
    30181 tttatgctca aatcgatcaa gcactagaag atggagatag ggatgctttt ttgaaattat
    30241 ctgatgaagt taaccgaaag atccttttgc agacacaaaa gaaaagtaac cgggtaagtg
    30301 aacataacaa ttagttaaaa atagtatagt actaaaaaag acaaaggtca gctggatgtg
    30361 gaatctttcc tcatagccta tctttgtctt ttttcttata gttttttcag taatcttgtc
    30421 tgtggcttca aataagtaaa gccttgccct tccacttcat gcacattgat gattgaaagg
    30481 aaagcttttt cgtcaatctc gtggacgatc ctcttagctt cagttaattc actagagcta
    30541 acgaccatat agatgatttt ttttgaatta ccagagaatg cgccttctcc agaaaggaaa
    30601 gtcacacctc tttgcagacc ggtcatcagt agtggtgcaa tctcttcgct tttgttggag
    30661 acgacaagta gaccttttgc agaatatccg ccatctaaga tcgaatcaat cacacgactg
    30721 aaaacatacg aaacaatcaa cgtatacatc atacgtttca agtcgatata ggttaatgaa
    30781 gcaagtaaga cgatgatatc aaatgccaat agggaacgcc ccatagtgat gccgtaattt
    30841 tgttctaaaa tccgagcgat aatatcactg ccgcctgttg ttccacccat gcggtagacg
    30901 atcccgcttc ctacacctgc aactaaaccc gcaagtagag aaacgatcag tagatcatgc
    30961 tgtaaattga tttctagtgg aaccctttgc cagatccata aaaagaatga caaagaaagt
    31021 gtacctagaa cagtataggc caaggaacgt ttaccgagga tctttcctcc tagtaaaatc
    31081 aatgggatgt tgattaataa agtcgtataa gctggatcaa taccaaataa agctcttaag
    31141 atcaaggtga tccctgagac tcccccttct gctaaatgat tagcgatgtt caagtaaaca
    31201 agaccaaatg cataaatact tgttcctata atgataaaaa gaacttcttt tactgttttt
    31261 cgtttgacca tatgaattcc agctttcaat tatgtatttt cacagaattt ccatttagaa
    31321 attccttcct aaatgtagca tggaaattcg attttctcaa ttgctcttat ctaggatttg
    31381 cgttaaagtt ttggtaagat agttttggag ggaaaaagat ggataaaaag cgttctttat
    31441 acagtatgca gcaggaagtg gatgaatata tccagcaatt caaggtgggg tatttctctc
    31501 cattagctca gatggctcga ttaactgaag aagtaggtga attggcaaga gaagtcaacc
    31561 acacttatgg agaaaaaagc aaaaaggctt ctgaaccagt taactcggta gcagaagaac
    31621 ttggtgatgt tttattcgtt acgatgatca tggctaattc attgaatatc gacttaactg
    31681 aagtttttga aaaaaacatg gaaaaattca atcaaagaga ccataatcgc tttgagcgta
    31741 aggacgagta aacaagatga agtggtcctg gccttgaaga ctctgaaacg gacttgtcaa
    31801 acagtcacca tgcaactgag tctttatctc aggacaagaa acacgcagga aaggttggaa
    31861 aaatgaaatt aaaacaatta cctctggaat accaaaaggc aattccagtt ctgaaaaaat
    31921 tagagaatgc aggatatgaa gcttattttg taggtggaag tgttcgtgat atcttgctga
    31981 accagccaat ccatgatgtg gatatcgcaa caagcgcatt tccagaagaa atcaaacagc
    32041 tttttccaaa aacgatagat attgggatcg aacatggaac tgtgttggta ttagaaaatg
    32101 atgaacaata tgagatcacc acatttcgga cggaatcaac ttatcaggac taccgtagac
    32161 cagatcatgt cgaattcgtt cgttcgttgg cagaagattt gaaaagaaga gattttacga
    32221 tcaatgcgtt tgctttgaaa gaagatggag aaatcatcga tttgttcgat ggtctgcaag
    32281 atctagaaga acagatcttg cgagctgtag gcaatcctca tgagcggttc catgaagatg
    32341 ccttacgaat gatgcgcggg ttgcgttttg tcagtcaact agggttcaaa ttagaggaag
    32401 aaaccttcta cgccatcaaa gaaaaccacg agctattagg taaaatatct gtagaacgta
    32461 taaatgtgga attcatcaag atgctcttgg gaaatgaacg ggccgcggga atccatgcat
    32521 ttattgaaac agagtgctac atttgctgtc ccggtttgaa agaagaaggc gaagcacttt
    32581 tacgttttgc tgaattacca tccgtcccat tgaaagaaga gagccaagca tgggcattat
    32641 tgatcagcca actggatcta aaagaaaatg agattcgttc ctttttgaaa tcgtggaagt
    32701 gttctaacca aatgattaaa gacgttcaag cacttgtcca aggactacga caacgagaaa
    32761 ctggagcttt ggaaccattc gaattgtact tcttaggcaa aaaacatgca ctgcaagtag
    32821 aagaattgat gatctatttt gacgaaacac ctcagatgga tgccgtagga gaatcctatg
    32881 atcgtttacc tatcaagtca ttaagcgaat tagcagtaga tggtaaaatc ttgctagaag
    32941 aaactggaaa aaaacctggt aagtgggtta gtgaagcttt gcaattagct gaaaaagccg
    33001 tcgtagaaca aagaataaaa aatgataaaa aacaggtttt ggatttttta tcgaaagaaa
    33061 aattgattta aaaaacacta gactgtatct gctcagcttt gttggctact taaagtgggc
    33121 agatacgtca ttagaagaaa aaacataaga tgtgaccact tacataaatt tgtgctaaac
    33181 tgaggtagta caagaataaa ttacatcata aaggagtctt tttaatgagt aaagaaatga
    33241 cagcgctaaa attttatttt agaaatggag aaacctggac gatcaaccgc cgtcatatcg
    33301 gcgatctttg gatcaaacaa atcacaacaa gttttggacg aatcaatggc agtgaatttg
    33361 ttgaaatcca tccgtgtgct ggatttaaaa tcgaaatctt ccatgaaggg gacgcagttg
    33421 cgacacatga tatcaaccta ggcggactgg aaatgggcat gtttaatcgc gcattgaaat
    33481 acgaagatat cgagcgaatg gaaatccttt accgaaatgg cacacctgat cttgtgtact
    33541 tcccatatct agataaagga acagaaggat tagacaatca atatcaatca acaaaaatca
    33601 gcgaaaaaac aggtaacttg tatatcgtga tcaatccaga tcaacgtgta gaagatgtat
    33661 acggggaatt tttcgaataa atcgattttt acgcaaaaat gaggctgtga aagagatgtg
    33721 tcttattact actgataggc tgtctcttcc acaccgttta ttcagaataa gggactgtga
    33781 caagattttt gtcgcagccc catttttttg ttgtgataaa agaacaagaa aggcaggaat
    33841 gggaaagtaa acatcctttt gtcagaaact tttatctttc ttaagtttta cttgatatct
    33901 gatactattt ggaatagaat gaggcgttga aaaatttatg gaaatttcta actagcaata
    33961 aacgatgaaa caatgaaata tttttcatcc aaaaagaact ttgctataat gatggaagtt
    34021 tgtgtatagg agatctggaa aatgagagag agttttgaac aacaaaaaaa actgttgcat
    34081 gatcgctatg gcgccctttc gatggatgac cgccgtcaaa tactttgtaa gttgcgtaaa
    34141 agaaatatat tgatgtatcg tcagttggaa cgtctgaaac atgatctgct tcgtttggag
    34201 tcaaaacgtg tacagtgtga attagagggg aaccaaacac aggttgaagt agtcgaaacc
    34261 aaaattttaa agaaaaaaga acaattttta aaaatgctta cacaaaataa aaaataaagg
    34321 ggatggacga tggaatttgc tgagttagtt attgtatttg cagtaacgat caccttatcg
    34381 aacatagcta gccgcatttt gccaatgatt ccagctccac tgatccaaat tttccttgga
    34441 gtggtattag gtctgacaga gtggggacaa tcaatcgatt ttgaacctga attgttctta
    34501 gttatgatta ttgcaccatt gttatttaga gaaggagaaa aagcagatat ttcttcgatt
    34561 ctcaaaaatt tcgggacaat tttgttcttg gcatttggag gagtcatttt gacccttgtc
    34621 ggggtaggtg cgacgttatc attcctgttg ccaagtgtcc cacttgctgc ctgctttgct
    34681 tttggagcag cgttaggtcc gacagatgcg gtagcagtca gttctctttc tggacgagtg
    34741 aatatcccta aaaaggcgat gcatattttg gaaggagaag gtcttttgaa tgatgcatct
    34801 ggtgttactg ccttccagtt tgctcttggt gcgctgatca ccggatcttt ttcagcagta
    34861 aacgcgggga tgagtctagt agtttctagt attgggggag ctttgatcgg ctttttgctt
    34921 gtctggttca aacaaaaaat aattcatttg atcgaaaaag catcagcaca agatgtcaca
    34981 gggtatctgc tgatcgaact tttgcttcct tttctagcct atgtattggc agaggcattt
    35041 gatgtatctg gaatcattgc tgcagtggca gcaggtattc tccaagcttc tggttttagg
    35101 aaaatctctg tgtttgatgc agaattgtcg agcttgtccc acagtacatg gacaactatt
    35161 gcttttacac tgaatgcatt agtatttata tttttaggaa tcgaattgac gcaagtgttc
    35221 tcgccagtat ggggagacgg tttatatcca aacggtcttt tattagcgat tatcgtatta
    35281 atctctgtca tgctttttgt gatcagattc atttctatca gtctttttta tgtattcaaa
    35341 gatggcagta aaaagttcaa aaaacagttg aacgagatct tgatcttaac atttggcggt
    35401 gtaaagggaa cagtcagttt ggctacgatt tttattttgc cattttcaat caataatatg
    35461 atgttttacc agcggtcttt gcttttgttt ctgacggcgg gtgtcatctt agtgactttg
    35521 gtcataggga tcattgtctt gcctatgctg acggaaacag aggaagcgaa aagcacggat
    35581 ttgaatgctt tgatgatttt agaagaggtc gttgaagttt tgcgaaaaga aataaaggaa
    35641 attgatcgta atacaaaaga atttttagcc actgaagcag tgatagaaaa ttatcaagaa
    35701 cggatacgtg atctgtactt ggaagattta accgatgatg aaaagcaaga agtacaagaa
    35761 atacaagcac tgattttgtc gatcgaacgg gatggattgg atgaaagtta tcgttcgggc
    35821 aagctttctt ctaatggtta tcgtttttac tcccgttttc tttctcgatt cgaacattct
    35881 atcacaagcc agattttgtc ttttatcggc ttttggttca ttgttgtccg taggttaatg
    35941 cgaatcgttc tccatccgaa aatgttttgg caaagaagat atgctgatcg tcagacattt
    36001 attagtgatg aagatatcca agaaatcaga gagacatatc aaaaaaatac tcagttgatt
    36061 attgaaagtt tagataactt gactgatatt tatgatgaaa cgatggttca attttttatc
    36121 cagcaacgca aacttgaagg aataaaaatg aactcgggga atttgatttc ttcgtggatg
    36181 atccagcaag atagtctgtt tacgaaaaaa atgctaagag gttattatct tgaaagaaaa
    36241 atcattgatg aatacgaagt tgctgaaaaa atcacgacgt tttctgcgaa cagctatcgc
    36301 aggaatatca atttgttaga atcttatacg atgaacaaac catctgataa cttttctttc
    36361 cgatttgcgt ttcgagcaaa gtcaaaaaaa acgagtccaa taaaataaaa aggtaaaaat
    36421 tttcaagttt tataaaaaac aaaacatatc agaacaggat cgaatcggtc ctgttctttt
    36481 tttgagaaca caaaaagaac aaataccaaa aaaattttcc tgaagttcac tatttttatg
    36541 atacaatatc caagataagg agggaacatg ttgaaagaat taaaagtaac tgacctcaaa
    36601 aaaacttatg gtgaaaaaga tttgtttgac cagatttctt ttttggtcca cgaaaaagac
    36661 cgtatcgggt tgattgggac gaacgggacg ggtaaaacaa gtctcttaaa tattattgcc
    36721 ggtatcgata gcagcgacgg tgatcggcaa acagtgtttt atccaactga ttaccgtatt
    36781 ggttatcttt ctcaagcagc tgaattttcc gatgaattaa ctgttctcca agcagtcttt
    36841 caaggaaaca gccccttgat ccaaactgtt cgagcatatg aggaagcatt gatcgaacta
    36901 ggggaaaacg gagaagaccc tgatgtgcaa aaacgttatg ctaaagcaga agaacaaatg
    36961 aataaagaag atgcttggac tacagatacc aacgcaaaga ttattttgca aaaattagga
    37021 atcagtgaat tagataaaaa aatcagtact ttatcaggtg gtcagaaaaa acgagttagt
    37081 ctggcgcaag tgttgattga tgaacccgat ttactattac tagacgaacc aacaaaccat
    37141 ttagattatg aagcgatcga gtggttggaa aactatttga aacaataccg tggcgcacta
    37201 ttgatggtta cccacgatcg ttattttcta gatagagtag caaaccgaat cttcgaactt
    37261 tcatttggaa aattatacga gtataaagga aattatgaag cttacgtctt agaaaaagca
    37321 gaacgtgatc gtgtagaagt agaacaagaa gaaaagcgca agcggcttta taaacaggaa
    37381 ttagcttgga tgagggctgg cgtacaggca cgtggcacca agcagcaagc acggatcaac
    37441 cgtttcgaag atctaaagga gaacttgtac caagtaaacc aagaagatga tttagagctg
    37501 aacctagcga cacaacgttt agggaaaaaa gtactggaaa tcaaagatgg cagttaccgt
    37561 ataaatgacc aaacactgct agaacattta gatttattga ttcaatctcg ggaaagacta
    37621 ggtattactg gtaaaaatgg tgcgggaaaa tcgacattgc ttaatattct agctggcaga
    37681 attcccctgg atagcggtac aatgtctatc ggtgaaacgg ttcatcttgc gtattataca
    37741 caagaaaatg aagaaatggc accagataaa cggatgattg cttacttaca agaagctgct
    37801 gaagaagcaa aaacagcaga tggcagtcaa atcggagtag cagaattact tgaacgattt
    37861 ttatttccaa gatttatgca tgggacactg ataggcaaac tttcaggcgg agaaaaacgc
    37921 cggttatttt tgttgaaact gctgattcag caacctaatg ttttattatt ggatgaaccg
    37981 accaatgatt tggacatagc aacattgacg attttggaag attattttag aagttttcca
    38041 ggtgctgtga tcactgtttc ccatgaccgt tatttcttgg acaaggtagc agataagtta
    38101 ttagtattcc aaggaaacgg aaaacaagaa ctttattacg gaaacatgtc cagttattta
    38161 ctgaagcaaa aagagaccca gcagcctgct gaaaaagcga aaccaaaaat acagagcaag
    38221 gaacctgctg aaaagaagaa attatcttac atggaacaaa aagaatggga aacgattgaa
    38281 gatgagattg cagaacttga agaaaaaatc tcattgctcc aagaagagat gaatcatcaa
    38341 ggagataatt tcactcgatt acaagaattg caaaacgacg tatctgaaac agaagcacaa
    38401 cttgaagaaa aaatggcacg gtgggaatac ttgagtgaat gggtagaaga ttaggaggaa
    38461 aaaggatgga acaagcgtat cttgacttag gaagaaaatt gttggaagaa gggcatcgta
    38521 aagaagacag aacaggcaca ggcacattaa gcttattcgg ataccaaatg cggtttgatc
    38581 tgcaaaaagg gtttcctttg ttaacaacga aacgagtacc ttttggattg atcaaaagcg
    38641 aattgctttg gtttttaaaa ggagatacga atatccgcta tctattacaa aataaaaacc
    38701 atatttggga tgaatgggca tttgaacgct acatcaaaag tccagactat aatggaccag
    38761 atatgactga ttttggccat agatgtttga cagatcctga attcaatgaa gaatataaaa
    38821 agcagagcaa gcttttttgc gagaagatcc taaccgatga ttcctttgct gaaacatacg
    38881 gcgatcttgg acacatctac ggctatcaat ggcgtcactg ggaaacaaaa gacggtggct
    38941 ttatcgatca aatcaaacaa gtgatcgaag caatcaaaaa gactcctgat tctagaagat
    39001 tgatcgtttc tgcatggaat ccggaagatg ttcctagcat ggctttgcct ccttgtcata
    39061 cgatgtttca attttatgtt caagagggac ggctaagctg ccaattgtat caacgcagtg
    39121 gggatgtgtt tctaggcgtt ccgttcaaca ttgcaagtta tgcgttgctg actcacctaa
    39181 ttgcacatga aacaggttta gcggttggcg aatttgttca cacattagga gatgctcatc
    39241 tttatatcaa tcatatcgaa caaatgaaag aacaattgag ccgagaaata cgcgcatttc
    39301 cgactttagt tttgaatcag gaaaagcagt cagtatttga ttttgagatg gaagatatcc
    39361 aattagaagg atatgaccca catccggcga tcaaagctcc tatcgctgtt tagaatcaga
    39421 aaggatgaga aaatgttagc tgcaatttgg gcacaggatg aaaacggatt gattggaaaa
    39481 gaagaccgtc ttccttggcg tttgccaaat gatctgaaat tcttcaaaca aatgacagaa
    39541 gcaaatacac tagtgatggg acgaaaaaca ttcgaaggga tgggtagccg tccgttgccg
    39601 aatcgtcaga cgattgtttt gacgagagat tcttcctata aagcagaagg cgtgcatgtc
    39661 atgcatagtg tggaagacgt gcttgctctg gaaaaagaaa cagacggtat ctttttcatc
    39721 gctggagggt cagctgttta tcaagaattt ctgccatttt gtacgatatt ataccgtaca
    39781 gtcatccatc atgtattcga tggagatgct tattttccac ctgttgactg gtctgattgg
    39841 tcactgatca atactagtca aggagaaatg gatgaaaaaa atcgttatcc gcatcaattt
    39901 gaaacgtatc agcgtaatga gaatcctgta aaaagcggga caggcgaatt gacgatataa
    39961 aaaagctatg gatagactag ctgttattta gagctgtccc atccatagct ttattttttt
    40021 tattaagtaa agaataaaac agagaagtac ataaaacctg cacctaacat aacaaacaaa
    40081 tgccaaacaa catgcatata gcggatagaa ttcaagctat aaaagaatgc gcctatcgtg
    40141 taagaaagcc cgcctgctac catcaaaata acaccagtca ttccaagaga gtgataaaga
    40201 ggaacagcag ccacgacaca gagccacccc ataaaaatat agatgatcgt agatactttt
    40261 gatactctct cccgtttatg caacgtcaac gctttatata taatgccaag aatcgcaaac
    40321 aaccagatta atacaaataa agtccaacct agccatcctc ggatactcag caaacaaaaa
    40381 ggagtataac ttcctgcaat caaaagaaaa atcgaactat gatcgaatac ttggaacacc
    40441 ttttttgctt ttgtaaaaat caatgaatga aacaaagtag acgttaaaaa taaaagaatc
    40501 atcatcgatc catagatagc ataagagaca atatggaggg aggaatgcaa gtgggcacct
    40561 tttactaaaa gaaccactag tcccgcgata ctcaatccag tcccaattcc atgggtgact
    40621 gcattaaaca cttcatttaa tatttcatac gttcgattaa atttcgtttt ttccatgaaa
    40681 acggctcctt tactaggtgt aattaatgaa aaatcattat ttgtctgtta tactaaaaat
    40741 agaaattgtc ttacatgtag tttaacatga ataaggcatc ttgttaaaga aagagagaga
    40801 gaaaataatg acaaatgtaa aaatcgtaac agattcttca tgtacgatgg aaaaaaatgt
    40861 acgcgatgct ttaggcattc gtgtcatgcc gctatccatc atgatcgacg gaacagtata
    40921 tcccgatgat gaccaactag acggcgaaag atttatggaa atgatggcga aagcaaaaaa
    40981 tctgcccaaa acaagccaac caccaattgg agaattcatc tcgatgtatg atgagctagg
    41041 agaagacggc agtgaagtga tttcgatcca tatgacaaaa ggcttgagtg gaacagtaga
    41101 agcagcaaga caagccagta atctaacaaa aacgaaagta acggttatcg acagtgatac
    41161 cactgaccaa ggattatctt ttcaggtaat tcgagcagct gaattagcaa aagaaggtaa
    41221 aacagtagag aaaatccttg aagaagtcaa aaaagtacaa gacaatacaa aattatacat
    41281 cggaatatcc acactagata acttggtaaa aggtggaaga atcagccgaa caactggttt
    41341 gctgtctagt gttttgaaca tacgcgtggt gatgaatttc gcccattctg aattaatccc
    41401 tgtaactaag gggcgaggaa agaaaacgtt cgataaatgg tttaataatt tgaaaaatga
    41461 actgaaagaa ttgccaaatg tgcgacaaat cggtatttca catgcggatg caaaagaatt
    41521 ggcgcaagaa ttcaaagagg gtttgcaaga attattcccg gacatgcata tccctgtact
    41581 gcatacgaat ccggttatcg ctacgcatac tggtaaagac gcgtttgcaa tcatgtatta
    41641 tgtagattag tcgaaaagag gataagccct cagcctgaaa ggcttgcagc ttattctctt
    41701 tttctttata aaattaggaa aataattgtt ttcttttttc ataaagacta cctttttgat
    41761 aaaatttttg ataacattag aaccaatcga atacaagagg tgaaaaaatg aaattaacga
    41821 aacgacattt gatcctcgtt ttgttcccat tattaattgc gatcatcatc tatgcaggtt
    41881 tagctgcttc tataccgaaa gctggcccac taatgaaacc agtaactact gtgagagaag
    41941 atccaaataa aaaagaaaaa cttcactaca tagctatcgg agattcgctg actgaaggag
    42001 taggagatca aaccaagcaa ggaggattcg ttccccttgt cgcaaatgat ctaaaagacc
    42061 gttataattt gaccgcggtt gaaacagaaa actatggtgt aaatggtgaa cgaagtgacc
    42121 agatcttaaa gcgagtaaaa acaaaagaag aaattcgaaa caatatcaaa acagctgatt
    42181 ttatcacact gactgttggc ggtaatgatt tgatgaaagt tatccaaaat aatttattcg
    42241 gcttaacggt caattccttt aaaaagccac tgaaaaacta tcaggaaaat gtcacagaac
    42301 tattggaaga atttcgatcc ctcaataaaa ccgcacctat ttacatttta ggcatctaca
    42361 atccatttta tttgaacttt cctgagatca ctgacatgca gacaattgtt aacaattgga
    42421 acaaaggaac agaaacggtg acaaaaaaaa tgccgaactg ctactttatc ccaatcaatg
    42481 atttgcttta tcaaggattg aatcaggaag taggtgtcac agaagaagga acagatcaaa
    42541 caactagcac gacagaaaat ctaaatggca tcaaaaataa tgtgctctat gaagaagatc
    42601 atttccatcc aaataattta ggttatcaat tgatggcaaa tgcagtgaga gatcagctga
    42661 tcgagacaca aaaccaatgg ttagagaagg aggatgatca ataaacatga aaacaagatc
    42721 tgacgtatct tcaaacaaaa caaagaaaac aaattactgg aaattcgcct tccttatcct
    42781 tcttggggta gtacttggtt cgaccgtatt tttaggcagc cgtatttttg ctaatcggga
    42841 accagaactc ccagaaatac cagctcttac ggaaagacaa ggagatcctg tcttaacgat
    42901 caattcaaat aaagaaaaag tcaatcaaat catcagcttt ttccttagtg agtatcaaaa
    42961 agatagtgat attgaatata aattttattt ggaaaatgaa gcactactca atggtacttt
    43021 cgaagtacta ggttttccta tcgatttcta tttatatttt gatccgtatg tgatggaaaa
    43081 cggaaatgtc caactaaaag ctagaagtct ttctatcgga acattgagtt tgccaatcaa
    43141 agatgtgatg aatatgatca agagaaacta taaattacca gattggatcg aagtcaatac
    43201 agatgatctg actatcatgc ttaggttgga tcaattccga atgcaaaacg ggatgtatat
    43261 taaagcagat aaaatcgact tagtaaatga tgacattcgg tttagtctat atttgccaaa
    43321 agatacctct gatgcttcaa acaactcaaa taccaacaaa actagcacca gcagtactga
    43381 aagcaagaca aaagaaaata aaaaatcatc ggatgagtag tattagaggg ggaatcataa
    43441 tggaacgagc aatatttgca ggaggctgct tctggtgtat gatccagcct ttcgacacgc
    43501 ttcctggtat ccatacagtg atgtcaggat atactggagg gcatgtacca gatccgactt
    43561 atgaacaagt aaaaagccaa acgactggac atacagaagc agtggagatt atctatgatc
    43621 caacattgat ttcatatgaa caattagtag aactttactg gcagcaaact gatccaactg
    43681 acgctttcgg acaattcgag gatcgcggtg acaattatcg cccggtgatc ttttatttga
    43741 cagaagaaca gcggatcatt gctgaaaaaa gtaagaaaca gttagctgaa agtggacgtt
    43801 tcgaacagcc aatcgttaca aaaatcgaac cagcccaacc attttatcta gcagaagaag
    43861 accatcaaga gtattataaa aagcatcctg aaaattttaa aagaaaccat gcacgccggg
    43921 ctgcctttat cgaagaaaat tggaggaacg aagggtgaat cgaagctttt ttcattattt
    43981 aatgacactg cgaggcggtg gcacacatga tgctttatct gttttcgcta cagaagccgg
    44041 taaagacatc caatttccta agcacagctc tagctacgaa gaaatttcta attatctgga
    44101 actgaatgtc gactatttgc cgtccatgga tatctttgac gaagcatggg aaaaatattt
    44161 agaaaataat catgacatat aagagatagt tttgtacttt atgatcaaaa aagttttttg
    44221 tccaatagcc taattgaaag aattattttt atttcagcta tctagtcgca tttttttgat
    44281 atttttctta taataaagaa agaatgaaaa aaagagggat ggattgatag agattgacaa
    44341 aaaaacaacg ctatattaat cgcttctggc ttgtattcaa atacttactt gtttctgtat
    44401 tccttgcgtt catgttgcgg ggatttttat tcatcccggt accagtggaa ggcaattcaa
    44461 tggaaaacgt gttgaagcaa ggtgatatgg ttgtgatgga aaaattttcg gagatcagac
    44521 gatttgatat tgttgttttt caattagcag atggcacgat ttatatcaaa cgagtaatcg
    44581 gtcttccagg agaaaatgtc agctaccaaa atgatcaatt gaagatcaat gggaaagtag
    44641 ttaaggaacc ttatctaact aaaaacctga aatcagatca tgcaaatgca tcctatacga
    44701 cggactttac actacaagag ctaacgggac aatccaagct tccggaagat aattattttg
    44761 tacttggaga taatcgaaga gtttcaaaag atagtcgttc gtttggcacg atcaataaaa
    44821 ctgatattct aggtaaagca agatttgtct attatccact tgatgaaata aaatggatcc
    44881 agtgagaaac aataaagcgt gcagtctgtt gagactgcac gctttattgt ttaatccaaa
    44941 atcagtttac taccttagta cgtaaaatca aacagctcgt ttaaacactt ttctagcctc
    45001 taaagttttg ataaatgaat aaacaaccat tatttgaata gggatttgga tcatattcct
    45061 gatgaatcta cctagaaaaa ttactttgaa atctgcacca aacaaaacag acatcagcca
    45121 agaattgata aaaaggctga ctgggatcat aatcaataat tgggcagcga tgacggaagg
    45181 caggtttatc tttttatgat ataaaaataa gccattaata aatccaccaa ggaaagccgt
    45241 gataagaaaa gcagcttgaa acgttcctga accaaatatc gtgaaattta aagtgttcga
    45301 caacatttca agcaaaccag cccagatcgg tccatataga taacccataa gcgccatact
    45361 taaaaatccg aatgtgattt gaaaataagc aggaccaatc gctatcttgt ataagatgac
    45421 atcaagggct aataaaagag cagaggtcga caacaatctt gcattcattt cgggcaatct
    45481 aaaaacgtct tttgcaatca aagtgttcaa aatattctct cctttgtcag ttaataataa
    45541 aaggatacca taaaatcacc aatgtgtcat gacacattgg tgatttacat acactggcat
    45601 ctaacaatta aaaaaagggt gagaagactg cttttataaa ctgtaaaaaa gagattgtac
    45661 gaataaaaaa aatagaggcg gagaatactt atcgtatcat ccgcctttgt ttttttgtta
    45721 taaaaaacgt cagattagtt atctgcttac atcttcatcc atacgggttc taatgtattt
    45781 ccgtctagat cttggacttc taatgaaaac atttcttctt caggcattcc catatctact
    45841 tcgtggaaag taccaccatt ttcttttgca atttgagcaa atttttttac ttcatccgca
    45901 ttttcaacac taaacgaaat caaagcacca cttgtttttt tattatcagc tatttcttta
    45961 tcttttataa attttttata aaaatcatga gagagaagca ttatccaaaa agtgtcatcc
    46021 cataccattg aaccagcttc ttcagtagaa aattcttcat tttttttaaa acccagcttt
    46081 tcataaaaag caacggaacg attgacatca gcaacaggaa aattaacaaa aaccatacta
    46141 ttcatttgtt tcgtctcctt tctgttttgt tcgtctatca tctcttattt aggaaacgct
    46201 gtcaaaaatt tagattcata tatagaaacc aaaataaaaa tttgcatcat caaaaaagtg
    46261 aagatgtgat agaatgagga ctggttcact ttcacttaac taatagatgg aatttcttta
    46321 taataaaaaa gtgaatttat attgaaaaag aaaaaaacaa gtaaagccgg ggaatattcc
    46381 ggtatgaaag gaatggagag aacaatgacc atccaatggt ttccgggaca tatggcaaaa
    46441 gcccgacgag aagtttctga aaaaatgaaa tatgtggaca tcgtatttga attggtagac
    46501 gcacgattac ctttatcttc tcgtaatcca atgctagatc agatcattca gcaaaaacct
    46561 cgccttgttt tattgaataa agcggatctg gcagatccac aacagacaaa gctgtggcaa
    46621 gaatattttc gtaaacaagg ccatcttgca ttagctatca cggcacaaga aagtaaagga
    46681 attaaaggtc tagtacctgc ggctaaagaa gcattgaaag aaaaacgggc aagagatgct
    46741 gctcggggga tcaagcctcg cgctattcgg gcaatggtct taggtattcc aaatgttggg
    46801 aaatctacgt tgatgaatcg actagtaggc aagaaaattg cacagacagg aaataagcct
    46861 ggcgtaacaa aaggtcagca gtggcttcgt ttaggtaatg agttggaatt gttagacaca
    46921 ccagggattt tatggcccaa atttgaagat gaagaaatcg gcaaaaaact agcgctgaca
    46981 ggcgcgatca aagaccagct tgtccattta gatgatttag cgatttacgg attagacttt
    47041 tttgcccgat attattcagg aagaatcagc gatcgttatg gtttatcaat cgagcaagaa
    47101 gaaaaattgg caccggaatt actgatggat attacagcaa aacgtgggta taaagaagac
    47161 tatgatcgtg gcagcgaatt agtcgtgcat gatattcgtc aaggaaaact tggacgatac
    47221 acactggatc gttgcgaaga aatggagcaa aaaagtgatg actgaatcca tccaaagtat
    47281 caaagaacga ttgaaaagca tacagagaat agatgatcct acactagaat cattaagaaa
    47341 tgatccacgc aaaggcgtgc agcaagcact ggcttctttt gaacgacgat tgaaaaagga
    47401 agcattaatt aaggaaaaat atgaagaaat gttttgcttt gaaaagcaag ggtacgcaca
    47461 aggtcaccaa tggattgccg gcattgatga agtaggaaga ggaccattag ctggacctgt
    47521 cgtggcagct gcagttatct tgccgaaaaa tgagatcata ttagggctga atgactccaa
    47581 acaattatca gagaaaaagc gagaacttct ttatcaacaa gttcaagaaa aagccctggc
    47641 aatcgggatc gggatcgttg atcagcagac aatcgatgat gtgaatattt atgaagcaag
    47701 taaattagcg atgatccaag cagtagaaaa attgtccatt gtaccaagct atttgttgat
    47761 cgatgcgatg accttggatt tgcctattca gcaggaaaaa ataatcaaag gggatgcacg
    47821 atctgtgtcg attgctgctg caagtatcat tgctaaagta tatcgagacc aattgatgaa
    47881 agaatatgac gtgctttatc ctggatatgg tttctcgcat aatgcaggtt atggaacaaa
    47941 ggaacactta gaaggacttg aacgacaagg aattacgcca atccatcgta aaagttttgc
    48001 acctgtaaaa aaatatttac aataaaaaac agccgctttt acgtatctta ttagaaagag
    48061 agacgaaagg cggttttatg atgtatcaaa tagaagaaaa tttattgaaa cttgcttata
    48121 caaaaggaat cagtagtcaa ggaaaatggg cacttgctaa ctggatgatg cagtatcctt
    48181 ataaagaaat cggatttgaa gacatcatta agataggtaa aataaccaaa caccgggagc
    48241 tgtttcgcaa aagttggcag gaaattcacg ctaactgggg caaaatccaa tcgaaacagt
    48301 cgtttatcac ttgctttgat cccaaatatc cccctcagct gcttcacttg acctatccgc
    48361 caattgtact gttttataat ggtgatctat ctttactttc ttgtaacatg ttaagcgttg
    48421 ttggtggaag acacacttct gctttagcca aaaaaacagt ttattattta ctaaaaccag
    48481 tgatagaagc tggatatgtg atcgtcagtg gctgcgcgaa agggatagat acatacggtc
    48541 accaagcagc aatctctcat ggtggacgga caatcgcagt cattggaaca gggatagagc
    48601 aagtctatcc tgctgaaaat cgtgacttgc aagaggtgat tgcaaatgat catcttcttt
    48661 tgagtgaata tcctcctcaa tccggcccga aaaagcattg ttttccaatg agaaatcgga
    48721 ttatcgcagc tttgtctaaa ggaacatgtg tctttgaagc caagaaaaaa agcggctctc
    48781 tgataacagc acagcaagca ttggatcttg gaagaacagt tttttctgtt ccaggaaata
    48841 ttttaacaga acattctacc ggatgtcatc atttgatcca agacggtgca gtttgcacgg
    48901 tttcgggtga agatatcttg gcagaattta aagaataaca ccatactttt catttgcttt
    48961 tcttgacaaa cgttttctga atggatatga ttagctacga tttgtatgaa tcctctttta
    49021 atagtaggaa cacgacaatg gaaaggagtc cattcagtaa tggcgtataa atatttagtg
    49081 attgtagaat cacctgcaaa agccaagaca attgagaaat atcttggacg aaactataaa
    49141 gtaatggcca gtgttggcca tataagagat ttaccgaaaa gtaaaatggg tatcgatttt
    49201 gaaaacaatt atgaacccca ttatatttct atacgcggaa aaggcgatgt catcaaaagc
    49261 ctgaaagccg cagcaaaaaa agctcaaaaa gtttacttgg caagtgaccc ggatagagaa
    49321 ggagaagcga ttgcttggca tttagcgtac ctacttgggt tggatctgaa agaaaaaaat
    49381 cgggtggtct tcaatgaaat cacaaaagac gcagtcaaag cagcttttaa ggaaccaaga
    49441 acgatcgatg tagatttagt agatgcacag caagctcgtc gtaccttaga cagaatcgtt
    49501 ggttattcga tcagtcctat tctctggcgt aaggtcaaga aagggttaag tgcaggacgt
    49561 gtccaatctg tcgctttaaa aattattatt gaccgtgaaa aagagatccg agaatttgtt
    49621 ccagaagaat attggagcat cgacggtaat tttaaaaaag ctcgcaagaa attcaaagca
    49681 aatttctggg gaatcgacgg taagaaaaag aaattaccag atgcacaaag tgtaaaagaa
    49741 gtcactgcta gaattgatgg aacagacttc gaagttaaaa aagtagaaaa gaaagagcgc
    49801 aaaagaaacc ctgcgaatcc tttcactaca agtagcctgc aacaagaagc tgcccgtaag
    49861 ttaaactttc gaacaagaaa aaccatgatg gtagcacagc aattatatga aggaatcact
    49921 ttgggtaaac aaggaacagt cggcttgatt acatacatgc gtactgattc tacccggatt
    49981 gccgatacag caaaagcaga ggccgcagca tttattgaag atacgtatgg aaaagaattt
    50041 tctagacatg atcaccgaaa gatcaaaaat gctcaaggcg cacaagatgc ccatgaagct
    50101 gtgcgtccaa cgagtgtgct tcgtacacca gatgaactca aacaatattt agataaagat
    50161 caattgaaac tttatacatt gatctggtca cgttttgtgg ctagtcagat gacaccagct
    50221 attttagata caatgaaagt gacattagaa caaaatggtg tcatcttcat tgcgaacggt
    50281 tcaaaaatca aattcaaagg attcatgcaa gtgtatgtgg aaggtagaga tgacggcaaa
    50341 gaagagaaag aaaatattct tccagaactt gaagaaggcg atgtagtcca atctgttgat
    50401 attgaaccaa aacaacattt tactcaacca ccagcacgtt ttagtgaggc tacgttgatt
    50461 cgtgcactag aagaaaacgg ggtagggcga ccttccactt atgcaccaac acttgatacg
    50521 atccaaagac gctactatgt aaaattgaca caaaaaagat ttgagccgac tgaactagga
    50581 gaaattgtca attcattaat ctgtgaattc ttccctcaaa tcgtcgatat ccatttcaca
    50641 gcagaaatgg aaggagattt ggataaaatt gaagaaggca cggaagcgtg ggtcaaagtg
    50701 gttgatcgtt tctataaacc gtttgaaaaa gaactgacga atgcggaaga aaaaatcgaa
    50761 aaaattcaaa tcaaagatga accagcagga ttcgattgtg atgtctgtgg acatccgatg
    50821 gtgataaaat tagggaaata tgggaagttt tatgcttgca gtaattttcc tgattgtcgg
    50881 aatacgaaac caatcgtaaa agaaatcggt gtgacttgcc cagtatgtca tgaaggacaa
    50941 gtcattgaac gaaaatctaa gaaaaacaga ctattctatg gttgttcccg tttcccagaa
    51001 tgtgaattca cttcttggga taaaccagtg ggcagaaatt gtccaaaatg tgacggatat
    51061 ctggtcgaga agaaagtcaa aggcggtaaa caagtagtct gtgtaaatgg agattacgaa
    51121 gaagacatcc aaaaataata gaaaagaggt tctttcattg aaaatggtaa atgttgttgg
    51181 agcagggctt gccggaagtg aagctgcttg gcagatcgca caagcaggag tccctgtccg
    51241 tttatatgaa atgcgtccag tcaaatcgac tgaggcgcat cacacaagta attttgctga
    51301 actagtatgt tccaattcat taagaggaaa cagtttggct aatgcagtag ggttattgaa
    51361 agaagaaatg cgccgattga attcggtagt aatcacttcg gcagatgaaa cagcagtgcc
    51421 agctggtggt gccttagctg ttgaccgcga ttctttttct gaaacaatta cccgaaaagt
    51481 caaagagcat ccattagtaa cagtgataaa cgaagaaatt actgaaatac cggaaggaat
    51541 caccgtagta gcaacaggac ctttgacttc tcatccatta gcagaaagca tcaaagcatt
    51601 caatggttca gatggttttt atttctatga tgcagctgca ccaatcgtag acaaagctac
    51661 gatcgatatg gataaagtat atttgaagtc ccgctatgac aaaggagaag cagcttactt
    51721 gaactgtccg atgacagaag aggaattcaa agctttccat gaggcactaa tcaatgccga
    51781 agtggcagaa cttaaatctt ttgagaaaga aaaatatttt gaaggatgta tgccaattga
    51841 agtaatggca cagcgaggat tcaaaacgat gcttttcgga cctatgaaac cagttggatt
    51901 ggaagatcct aaaactggca aacgtcctta tgctgttatc cagctgcgtc aagacaatgc
    51961 tgcagcttct ttatacaaca tcgtgggctt tcagactcac ttgaaatggg gcgagcaaaa
    52021 acgtgttttc cggatgattc ctgggttaga aaatgctgaa ttcgttcgct atggtgtgat
    52081 gcaccgaaac agctttatga attcgccaga attgctgaaa ccgacttacc aatcaacgaa
    52141 acgcgaagat ctgttctttg caggtcagat gactggtgta gaaggctatg ttgaaagtgc
    52201 agcgagcgga ctacttgctg gaatcaacgc cgcacgctta gcaaaagaaa tggaaccagt
    52261 cgagtttcca caagaaacag ctattggaag tatggcttac tacattactc atgcggaagg
    52321 aaaacatttc caaccgatga atgccaacta tggtctgttt cctgaactgc cagagcgcat
    52381 tcgagacaaa aaagcaagat atgaagcaat tgcacaacga gctttaaaag ctaatgaggc
    52441 aataaaaaca gaattagaaa aaactgcaat cccaaatgag taaggaaact ccatgaagag
    52501 ggtgagacgg atagtctcgc tctttttctt gcgtttttct taagaatttc tcaaacaaaa
    52561 tgtgaggaat tgcgccaatt gtgaacaaac ttagaaatat tggttaaatt ctgacaattt
    52621 atttgtcttt ttataaacgc tgtgctaaat tattctctgg gacaaaagga aggtgagaaa
    52681 atgacagaag ccacgtggga acaaaaattt ttgcgttatt tgattgtcga aagaggatat
    52741 tcagaaaaaa cgaaagaagc atatgaagaa gacattctcc atttcaaaca ttttttggag
    52801 gaatctggag acgctgatct gctgaaggcg gaatatttta cggttcgtgt ctatttgagt
    52861 gcattagctg atcatcgtcc tagctatagc agaaattcta tcagtcgcaa gatagccagc
    52921 ctacgttcgt tttatcagta tcttctcaaa gaagaagtga taaaagaaaa tccattttct
    52981 tacgttcatc tcaaaaagaa aaatttgaga ctgccacgtt ttttttatga aaatgaaatg
    53041 caagcacttt ttgacagcgt agcaggagat agtcctttgg atttgaggaa ccgcgcttta
    53101 ctggaagtcc tttacgggtc aggcatccgt ttaagtgagt gtagcagttt gtcggtatca
    53161 gacattgatt ttgattcaga ggtcatgttg atccatggta aagggaacaa agagagatat
    53221 gctcctctag gttcttttgc tcaagatgct ctgcaagaat atttcaggga aggccgccag
    53281 gtattgatga caaaatatca cgaagagcat gatatcgttt ttgtgaatca tcatggaaaa
    53341 ccaatcacgc ctactggaat tgaatatgtc ttgaatcaaa tgatcaaaaa aagcagtttg
    53401 gatagtgata tccatcctca catgctgcgc catacatttg ctactcattt attaaataat
    53461 ggggcagatt tgcggacagt ccaagaattg ctaggacatg ccaatttatc gactacccag
    53521 atctatgccc atgtaacaaa agaaagttta caaaaaaact atcgttcttt ccatccaaga
    53581 gcgtaaaaat aaggaaaaga acaaatttag gaggaaacaa acatggtcga atcaccattt
    53641 cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat ttgctatggc tggagatggc
    53701 caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg caaagaaagt acgacgtatc
    53761 tacaatggag aagttgttgt aggtttcgcc ggaagtgttg cagatgcgtt tactttagaa
    53821 gaaaaatttg aaggcaaact aaacgaatat aacgggaatc tacaacgcgc agctgtcgaa
    53881 ttagctcaag aatggcggac acagcaatct atgcaaaaac ttgaagcgat gctgatcgta
    53941 atgaatgata aagaaatgct tcttgtttca ggtacaggag aagttatcac accagatgat
    54001 ggtatcttag ctatcggatc aggagggaat tttgcgttgg ctgcagcccg agcaatgaaa
    54061 aactatgctc aaaacgacat gagtgcgaaa gaaattgcag aaaatgcgtt aaatattgcc
    54121 gcagatatct gtatctttac taatcataat attatcgtag aagaactgta agaggtgagt
    54181 agatatgtat gaattaaaac aaacaccaag acaaatcgta agtgaattag atgaatatat
    54241 cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct ttgcgtaacc gctatcgccg
    54301 tttgcagtta gatgaaaaaa tgcaacaaga tattacacca aaaaatatgc tgatgatcgg
    54361 acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg gcaaaaatcg tcaatgcacc
    54421 atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt tatgtcggaa gagatgtaga
    54481 atcaatggtt cgggatttag tagaaaatgc gatccaaatc gtagaaaaag agcagtacag
    54541 ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt ttggtaaaaa tcttggtacc
    54601 tggtatcaaa aaagaacaga aacaatccac taatcaattt gaacaaatga tgaatctttt
    54661 taatgctagt cagcaaaata ccgctgaacc gcaagaagaa gtgacagaag atattcgaat
    54721 caatcgccgt acagtcttgg aacaactaca aaagggttta ctggatagtc gtgaagtaac
    54781 cattgaagtt gaagaccaaa agaaacctgc accaatgatg aacaatggat tggagcaaat
    54841 ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg aagaaaaaag tggaacggac
    54901 ggtaacagtc aaagaagctt tagagttatt gatcaaggaa gaatcagcaa aattagtcaa
    54961 ggatgctgat atccatagtg aggcaatcaa attagcggaa gaaagtggaa ttatttttat
    55021 cgatgaaatc gataagatca cttctaaatc acagcaatct ggagaagtat ccagagaagg
    55081 cgtccaaaga gacatattgc caattgtcga aggttcacaa gtaaacacaa aatacgggat
    55141 catccaaaca gatcacgtcc tattcattgc ttcaggagct ttccatttgt ctaaaccaag
    55201 tgatctaatt ccagaacttc aaggacgctt cccgatacgg gtagaattag atgatttgac
    55261 agctgaagat tttgtgaaga ttctgaccga accaaataat gctttagtaa aacaatacgt
    55321 cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca aaagaagcaa ttgaacggat
    55381 cgctcaaatc gctttcgatg tcaaccgtga cacagataat atcggcgcac gcagactgca
    55441 taccattttg gaaaaactac tagaagatct attatttgaa gcaccagata tgcaaatggg
    55501 agaaataacg atcacagaag catatgtgga tgacaaactt ggctcaattg cggcaaatga
    55561 agatttaagt cgctatattt tataaaaaat cggaggacga caatgacaga cttgctaacg
    55621 aaaacccgca agataaacac acttttgcag caaaagaata cttttgacat gcaagcagat
    55681 ttgccttacg ataaaatggc tgtcacacta ggtggtattc ttgacagcaa cacctatatc
    55741 ataggaagtg acggagtatt actaggttat aatgaacgtc acgatgtgaa caatgaacga
    55801 atcaaaacaa tgtttgttga gaaacaattt cctgaatctt atactgatac agtggcacag
    55861 ttgatgaaaa ctgaagctaa tatcccaatt actagtgata ttactgcatt tccggtagag
    55921 tcaagagaga aatatccatt tggtctgacg actgtcgttc cgatctttgg agctggcgaa
    55981 cgtttgggta cgattatctt atctcgtatg gaacaatcgt ttgcagatga cgatctagtc
    56041 ttagcagaat atggagctac ggttgtcggt atgcagatcc tttatcagaa gtctcgaagc
    56101 atcgaagcag atgtacggag tgcaacggct gtccaaatgg caatcaacac attatcttat
    56161 agcgaattga aggctgtaca agctatcttt gaagcattgg atggagatga aggtcgtctg
    56221 actgcttcta atattgccga tagtatcggc atcactcgtt ctgtgatcgt caatgcttta
    56281 cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt taggcatgaa gggaacttat
    56341 ttaaaagtat tgaattcccg ttttaaagac gagttagcaa aacacagtta ttagaagtaa
    56401 ggggactggg gcagatagaa atgtttgttc cagccttccc attttaaaaa gtaagtcgaa
    56461 gttggaggaa tggcaatgac tgttattatt caaaatgacc agctgatcgc cgaaatcgct
    56521 gaacacggtg cggaattgat cagtctgaaa tcaaaagaac atggtttaga gtatatctgg
    56581 caaggtgatc cagcgtattg gggcagacat gcaccggttt tattcccttt tgttggacgt
    56641 ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc aaatggggca acacggattt
    56701 gctcgggata tggattttga agtcattgaa catggacaag aaatggctag tttcatctta
    56761 aggagtacgc cggagacatt agaaaagtat ccatttgatt ttgaattagt gatcagctat
    56821 gaattaggag gagacggcat cacggttcat tatcaagtgg caaataccgg tgaaaaagaa
    56881 atgttctttt ctatcggtgg acatcctgct ttcaatgtac ctcttgaaga aggattgtca
    56941 tttgaagatt tctatctggc attttctcca ttgaaatcaa gattacgcct accattgaaa
    57001 ggcccattca tcgatatgga tcaaaagacg ttaggtcaga caaatacgaa tctggctttg
    57061 aatcgtgaac tttttgagca agatgccttg gtttttgaaa caaggggtct aaatgcgtac
    57121 gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa gctataaaga tatgccttat
    57181 gtagggatct ggtctcctta tccaaaagaa gcgcctttcg tttgtatcga accgtggtgc
    57241 ggaatcgctg acacggtgaa cagtagtgga aacttgatgg aaaaagtagg gatcaatcaa
    57301 ctaggtgccc atgaaatctt caagacaaaa tattccatca cagtgaagta agaggttgtg
    57361 accgagaagt tagcagggaa caccgtttac caaggtgtgt aaaaaagcta tcctgtaagt
    57421 aataagggag tgtgacatcg atttgtcaca ctcccgtttt atctctttgg aatgaaaaag
    57481 tatcaggatt ttttcttgct gctattcaaa ccaaacggga ttcgactttc agtatggttt
    57541 ctgatacgct tgatattgtc tttatgccga taaaagataa aggaagaaac taaaaaggca
    57601 atgatcgtca gtaaccaatt gggcttagcc aaaatcacag gagctacata aggaaggacg
    57661 atcgtagata aagtgatcaa tactgcactg atcatactgc tcaaactcac catacttgta
    57721 caaaataaac taacgataaa gatgatacaa gaataaacaa aaaatacagg attaaatcct
    57781 aaaagcattc ctgcacttgt cgcaacagct tttcctcctt tgaacccagc aaaaatcgga
    57841 aatgtatggc caagaatggc gcatacccca aaccaaagag ggttaatccc agaaaggtgg
    57901 aagatcatcg gcaaacaagt agccaatgta ccttttaaaa tatccataag caggacaaca
    57961 atcccggctg ttttgcctaa tacacgaaat gtattggtcg ttccagtatt accgctcccg
    58021 tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc agacaccgga aggaatagaa
    58081 cctaaaagat atgctaaaat aagtaataag atcaatttca ttttttaccc tctttcaagt
    58141 tgaaaactat cctttatttt agcatgaata ggtaacagga acaattgaaa aaatgaaaga
    58201 tacaagtata atagatggaa taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa
    58261 atccaacaca agaaacgatt ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag
    58321 gcttgagcaa taaaccagaa cgaacaagtt acatggttgc acaggtattg caagacaatg
    58381 gatataaaat cataccagta aatccattat tggctggtga agaaattcta ggcgagaaag
    58441 tctatgcgaa tttgacagaa atcgatgatc caatcgatat cgtagatgta tttagacgga
    58501 gtgaattttt acctgaaata gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg
    58561 cacaattagg gattgaaaac aaagaagcag ctgagatgct aagaaaagca ggacgtacag
    58621 atatcgtaat ggatcgttgt attaagattg aacttggtaa gttgaaagaa aaccaaaaat
    58681 aaaaatgacc acaaagaaga gatgatcgat agaggggaat gtcgaaaaag cggttaggag
    58741 ataattaaaa ttgatttctc gtctaccctc ttttttttca cgaccttttc tagtatgata
    58801 atcgtggtgc ggttttgccg caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag
    58861 taaacaacga atacaatgac tcgtccatcc aagtattgga aggattagag gcagttagaa
    58921 aaagaccagg gatgtacatt ggatccactg acagccgagg cttgcatcat ttagtctatg
    58981 aaatcgtcga taatgcagtt gacgaagcat tatcaggtta tggcagtgag attgatgtga
    59041 ccatccatga agacaatagt ataactgttg cagatagcgg acgtgggatg cctgtaggaa
    59101 tgcatgcttc cggaatacca acagttgaag tgatttttac tgtgctgcat gctggcggaa
    59161 agtttggtca aggagggtac aaaacttctg gtggactgca cggtgtagga gccagtgtgg
    59221 taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag agatggcgta gagtatcagc
    59281 agaaatttaa aaacggcggt aaacctgacg gtactttgaa aaagatagga aaaacaaaaa
    59341 aagcaaacgg gacgactgtc cattttttac cagatgatac aatattcagt acaacgaaat
    59401 tttcatatga aattttagcc gaacggttaa gagaatctgc ctttcttcta aaaggcgtta
    59461 aaatctctct ttctgatctt cgtggagaag aaccagtcaa ggaaattttt cattacgaag
    59521 aaggaatcaa agaatttgtt gattatctga atgaagaaaa agatacattg acaccagttg
    59581 tttatttttc aggagaaaaa gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg
    59641 gttattcaga aaatgtttta tcttttgtca ataacgttcg gacaaaagat gggggaaccc
    59701 atgaagctgg tatgaaagcg gcgatgacga aatcttataa tgaatatgcg agaaaagttg
    59761 gtttgctgaa agaacgggac aagaatctgg aaggtagtga tttcagagaa gggttagctg
    59821 ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa
    59881 aacttggtac gccagttgct cgtaccgtag tagataacgt catcagtgaa caaatgggct
    59941 tttatttgca ggaaaatagc gaaatgagcc agatgttggt ccgtaaagca atcaaagcca
    60001 gagaagcgag agaagcagca cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta
    60061 aaaaaggcga atctcttctt tcaggaaaat tgacacccgc acaatcccga aaccctaaga
    60121 aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg atctgctaaa caaggaagag
    60181 acagaaagtt ccaagcaatt ctacctttac gaggaaaagt catcaatacc gaaaaagcta
    60241 aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat gatctatacg attggtgcag
    60301 gagtcggtcc tgagttttct atcgaagact gtaattacga caaagtaatc atcatgaccg
    60361 atgcggatac agacggcgcc catattcaag tattactgct tactttcttt tatcgttata
    60421 tgaaaccgct gattgaagct gggaaagtat atatcgcgtt accgccttta tataaagtat
    60481 ccaaaggaca aggaaagaag caagtcattg aatatgcgtg gacagatgac gaattggcag
    60541 caatgattaa aaaagtcggc aaaggatata tgctgcagcg atataaagga cttggagaga
    60601 tgaatgcgga acaattgtgg gaaacaacga tggatccgac ttcccgtacg ttgatccgcg
    60661 tccgtatcga tgatgctgcg caagctgaac gtcgagtaac cacactgatg ggtgacaaag
    60721 tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca
    60781 gtatcttaga taaaaaagag gatactgaaa tctctccttc agtttctaat gatttattag
    60841 atgaagaacg agcagataaa aatgaaaaca atcagttatt tgaagtagaa taggaggtca
    60901 cctgatggaa aatcgacaag aagtacaaga attaacttta gaagaagtga tgggtgaccg
    60961 cttcggacgt tattccaaat atattatcca agaacgtgca ctgcctgata tccgtgatgg
    61021 attgaagcca gtgcaacgcc ggatattatt tgccatgaac aaagatggca atacgtatga
    61081 caaaggattc cgtaaatcag caaaatctgt cggaaatatc atggggaatt atcatcctca
    61141 tggtgacatc agtatttatg aagcgatggt ccgcatgagc caagactgga agttgcggga
    61201 agtactgatc gaaatgcacg ggaataacgg gagtatggac ggtgatccac ctgctgcgat
    61261 gcgttatact gaagccagat tatccaagtt aagtggagag atgctagcag atatcgagaa
    61321 agaaacagtc gatctcgtgt ggaattttga tgatacagaa aaagaaccaa cagtccttcc
    61381 agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt atatctgctg gatatgcaac
    61441 agaaatcccg actcataact tagctgaagt gatcgatggc acaatctata tgatcgatca
    61501 tccacaagcg agccttgaaa aattgatgga atatattcct ggacctgact ttccaacagg
    61561 agggatcttg caaggaaaag atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt
    61621 tattcttcgt tcgaaaacaa agatagaggc aatgaagggt aataaacagc aaatcgtgat
    61681 cacagaaatc ccatatgaag taaataaagc gaccttggtg aaaaaaatgg atgagatccg
    61741 tttgaataag aaaatcgatg gaatagctga agtacgagat gaaagtgata gaacaggtct
    61801 gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa ggaattttga attatttgtt
    61861 caaaaacact gagctacaga tcaattacaa tttcaatatg gtcgctatcg accatatgac
    61921 tccgcatcaa gttggactga aagacatttt aagaagttat atcgagcatc gaaagcaagt
    61981 cataacaaaa cgtagtcaat tcgatttagc caaagctcaa aaaagacagc atatcgtaga
    62041 aggattgatg aaagctttat cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa
    62101 agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt caatttacag aagaacaagc
    62161 agaagcaatt gtcacattgc aattataccg tttgacgaac acggatatca cagagcttca
    62221 aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg aataaaatcc tttcaaatga
    62281 caaagagctt ttctctgtta tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc
    62341 cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc aaaattgata cgcaagtctt
    62401 agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag
    62461 ccttcgttct tatagtgctt ccaaaccaga agaaatcggg atgcgagaag gcgactactt
    62521 gctatactca ggtgaattga gtacattgga ccatgttctt ctgatcacaa ataaagcaaa
    62581 tgtgatctac cgccctgtcc atgaactacc cgatttgaaa tggaaagatg ctggtgagca
    62641 catttctcaa acaatctcca atctgtctgt agacgaatcg attttagctg ttttcccata
    62701 tcaaaaaatt caagcagaga aaacatttgt attcatcagc aagaatggat tgatcaagca
    62761 aacacgaatg acagagttcg aaccatggcg cacctataag agccgtccgc tcagctgtat
    62821 gaagctaaag gcggaagaag atgaactgat tgctgtgtat ctgaaagaag atcagccagg
    62881 cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg cgctatcctt taacagatgt
    62941 tcccgttgtt ggagcaaagg cagctggagt gaagtctatg aatttgaaag atggagatag
    63001 gattatcaac gggctgcttg tgttagcaga aggtgatacg ccgatcgtaa tcgttactca
    63061 gcgaggagct gttaaacgca tgcttgctca agaaatcagc caaacttcac gtgcgaagcg
    63121 gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac cgtattatct atatgtcgga
    63181 aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc
    63241 aactgatttc cctataggtg atcgaacatc gaatggatca tttgcgatgg atgagaaaaa
    63301 aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt
    63361 gtagtatttt aatgaagaga cgccatctgt attaatacag gtggcgtctc tttttcattc
    63421 atttttaata aatccttata cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat
    63481 gttggtatgt caacgttgat ttaaatatat gtaaataaaa cataaaaatt gttaaataaa
    63541 aaacaagaaa acccttgcaa agatagggtg ctgtgttata ctaatcacgt aaaaactgtt
    63601 acataaaaca gaaatttagg aggtccatac aatggagcaa tggaatggat ttaaaggtac
    63661 caaatggaaa gaaggcgtag acactagaga ctttattcaa aataactaca ctgaatatcg
    63721 cggtgatgac agctttttgg aaggcccaac agaagcaaca gaaaaattat gggatgaatt
    63781 gcaaaagtta aatgatatcc aattcgagcg taacggtgtc tatgatatgg acacagaaat
    63841 cgtatcaact attacttcac acgatgcagg ctatttggat aaagatttgg aaaaaatcgt
    63901 cggtctacag acagacaaac ctttgaaaca agcattcatg ccgttcggcg gaatcaatat
    63961 ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac gatgaattga cacatatttt
    64021 cactgactgg cgcaaaacac ataatcaagg tgtatttgac gcttatactg ctgaaatgcg
    64081 tgctgctcgt aaaaacaaaa tcattacagg gcttccagac gcttacggtc gcggacgtat
    64141 catcggtgac tatcgtcgtg tggcattgta tggaatcgac tatttgatgg aacaaaagaa
    64201 aaaagatcat gatatgactg gttatcatac aatgagtgaa gaagtgattc gtttacgtga
    64261 agaaatcact gaacaatatc gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg
    64321 ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg
    64381 atatttagca gcaatcaaat cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc
    64441 attccttgat atctatatcc aacgtgattt ggataatgga gtaatcacag aaaaagaagc
    64501 acaagaattg atcgaccact tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc
    64561 tgaatataac caattattct caggattccc tatctgggct actttatcca ttgcaggtat
    64621 gggattagat ggtcgttcat tagttacaaa gaatgatttc cgtctgcttc atacattaac
    64681 aaatatggga ccttcaccag aaccaaactt gactgttttg tattctgaac accttccaga
    64741 aggattcaga acatatgcta gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa
    64801 tgataatcag cttcgtgaac aatggggttc tgatgattgt gcgattgctt gctgtgtatc
    64861 aggaacagtt gtcggaaaag acatgcaatt ctttggcgct cgtgctaact tggctaaaac
    64921 actattatat gccatcaatg gcggagtcga tgaagtgact ggcgcccaag ttggaccaga
    64981 atatcgtcca atcacatcag aaatactaga ctttgaagaa gtgaaacata acttcatgaa
    65041 tatgatggat tggttagcag aactatatgt caatacgttg aacatcattc attatatgca
    65101 tgataaatat tcttatgaag cagctcagct ggctttgatg gattctgact tgcaacgcac
    65161 atttgctaca ggtattgctg ggatctctca tgcggcagac agcttgtcag cgatcaaata
    65221 tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca gttgattttg aagtggatgg
    65281 cgacttccct aaatacggaa atgatgatga ccgtgcagat gaattagttt catggatctt
    65341 ggaatacttc atgggacaaa tcaaacgtca taaaacttac cgcaactcaa aaccaacaac
    65401 ttcattattg acgattactt caaatgttgt ttacggtaaa aatacaggaa atactcctga
    65461 tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat ccaagttaca atgcggaaca
    65521 aagtgggtta ttagctagcc tgaactcaac agctaaaatg aattacggct gtgcattgga
    65581 tggaatttca aatacccaaa caatcaatcc ttcaggtctt ggaaaagaac aagatacaag
    65641 aatcgaaaac ttgcgtaatg tcttagatgg atacttctca aaaggagctt atcatttgaa
    65701 tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc
    65761 gaacttgacg atccgggtat ctggttatgc agttaaattc cgtgacttga caagagaaca
    65821 acaattagat gtcttgatga gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag
    65881 tttttcgact tagcgtcgaa aggacgttga atagccatcg catcaaaatc gaataatcag
    65941 tatgcttatt tgaaagaaac gatttacagg ggcaggactt gtatgttccg tccctgtaaa
    66001 ttttacacat aacgaagcgg caattgcgaa aaattttgtt cagaaagctt cgcttcgaca
    66061 gctaacttgt acttaaaatg aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac
    66121 aatcggctat gttcattcta tcgaaacttt cggttcagta gacggacctg gactgcgctt
    66181 tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt tgtcacaatc cagatacttg
    66241 gaatattggc ggagggaaag aatacacagc agatgaactt ttagataaag cagaacgctt
    66301 cagaccttac tggggagata aaggtggtat tactgttagt ggtggagaac ctctattgca
    66361 gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag cggaagatgc atactacatt
    66421 ggatacatgc ggaaaacctt ttacgtatga agaacctttc ttttcacgtt ttcaagaatt
    66481 gatgaagtat acagatttac tgttatttga catcaaacac atcgataatg aagagcataa
    66541 aaaactaacg cattgggaca atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat
    66601 caacaaaccg gtttggattc gccatgtact agttcctgag cgaagtgatt atgatgaata
    66661 tttgatccga ctagacaact ttattcaaac cttgtcaaat gtagacagag tagagattct
    66721 tccttatcat acgatgggaa aatacaagtg ggaaacactt ggtttgaaat atccattaga
    66781 agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga
    66841 agagtataca aaatatttaa cgcgataggt cgcatcggaa agaagataag tattgcttct
    66901 ttccgttttt ttgctattat taaataggag aaatcacaaa ggagcgaatg acatgtcaaa
    66961 aattttagtt tttggtcacc aaaatccaga tacagatgca atcggggcag ccattgcgtt
    67021 ttcacacttg caaaatgaat taggtaaaga aacagaagca gttgctctag gtacacctaa
    67081 tgaagaaaca caatacgcat tagaccactt tcagctatct gcaccacgag tagtagagtc
    67141 agctaaagca gaaaatgcag atgtcatgct tgtggatcat aatgaattcc aacaaagtat
    67201 ttcagatatc agcgaagtaa atattttagc tgtagtagac caccatcgta ttgcaaactt
    67261 tgaaacagct aatccattgt actatcgtgc agaaccagtt ggatgtacaa gtacgattat
    67321 tttaaaacta tataaagaaa accaagtaac aattcctaaa gaaatcgcag gaatcatgct
    67381 ctcagctatc atttcagata cattgttgtt caaatcacca acatgtacgc ctgaagatga
    67441 agcagctgca aaagaacttg cagcaatcgc tgaagtcgat ctaaacactt atggacttga
    67501 aatgttgaaa gcaggaacaa atctaagtga taaatcagct gcggttcttc ttgatttgga
    67561 tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc gctcaagtca atacagtgga
    67621 ccttaatgaa gtgatggatc gacaagcaga gctagaagca gcaatggaag atgaaatgaa
    67681 agcaaatggc tacgatctgt tcgtattagt tgtcacaaac atcttagaca gtgattcaga
    67741 attacttgtt gctggtaaag gaaaagaaaa agtagaagaa gctttccaaa caactttgac
    67801 caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt
    67861 gacggctgta tttagctaat caaatggtag tatgaagcat tcaaaatgaa tggataagcg
    67921 attgatataa aaagaaacat agaacatact aaaggccgaa gaaatggagg agaataacag
    67981 tcaattatga ctcttgttct tcctcctttt tacgtaaaag ccttttaagt aaaataaaac
    68041 ggatagaaaa gagaacatat atgaactatt attcaaaaaa taaggaaaac ttttatcaaa
    68101 aactgaccga tgatccgctt ttttcttcat tgaccgatta tctatatgaa catcgagaac
    68161 aagaaacgat tctaagagaa ttgaaaaaag aattttcgca aaataaattt tcccattttc
    68221 ttgatttatt gatcgatgca ggattaatca agagggaaga acgaagatat catttgaact
    68281 ttccaatatt tgatcctaaa gattacttgc aacaagccac ttcggctgct gaaaccatag
    68341 ctgaacaatt gaagagactc tctgttgatg aacaaaaatt agcgatggga gaagttattt
    68401 gggcgtattg tttcgaagat gaacggaaag aagcgtattt ttatggagta agaaacagcc
    68461 gagagacaga attgctgagg acaactgcag gaaatgagaa gtaccgtttt atcacacttt
    68521 cctctataga acacttcccg cttactttgg ctaattactt ttttgtacag aagaatcagc
    68581 ttcctgtaac gaaggcattc aaagaattag ccgaacttat tggagacgta aatgaatcgt
    68641 acttttttga tcaaatagaa gttatcgttg acaggatacg taagaataaa tataaaaatc
    68701 gccggcctag tattttccat cagtctctac ttgtgacgaa tactataaag gaagaagaat
    68761 cattcacatt agaattacct attgtcgaaa aaaacaattt tgagattgaa ttaccaacat
    68821 tggatccttc attgacaatg gaggaaatgg cctttcttaa acgacaaatc ttttctgagc
    68881 tgagcaagaa atttataccg catgcatttt cttatatcaa agaatatgga acagttttag
    68941 tgagtaaaac ctaagagagc agtaagcaaa aattatactg actcttgcag gaggagcctt
    69001 tctcttcctt tatggttaaa agaattattt gagtattttg cctatctacc acctattaga
    69061 ttcactacat ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca
    69121 aaggaagtag tgataagaaa agttagattt ttaaataatc aatattatga ttcagtcaag
    69181 tatggcattt tatgggaaga actagctgat tagattagga agtccatttg ataatccaga
    69241 atcactaaga aacgaatggc gagtcattaa taatttatcc aatcttgggt tattctttta
    69301 gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat
    69361 gctttttatt ttgtttgttt tttctttgcc acataaatac acctagactg acgatgaaga
    69421 gcatatccaa cagatttcct gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag
    69481 caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt gacaggaact aatcgaaaga
    69541 agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta aaaatggata
    69601 aaatcacgac taaaccattc atgataaaga agaaaagcgt attgtgatta aacgaataaa
    69661 aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta
    69721 acgtaccagg atgtcgatga aaaaaatagg caccgaaata aacagaaaac agtaagccgc
    69781 aaagagaaga caaaatcaaa aaaaacggat tttcttcggc ttctgtgata tcagaatgat
    69841 ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga ggtcttaagg tatgaataac
    69901 atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg
    69961 ttctacgttg aatttccata atatctccaa attaaattga taagtatata aagtataata
    70021 acaaattgat gagtatatgt cgatgttttt taatcaaaat aataaataaa aactgtctaa
    70081 catttaaaat ttaatttaga tattagacag tttttaagtt tgtgagttat tttccttgtc
    70141 gttttctcac ttttttagtc cagaaaccac ctgaaatata ttttggctca taagaaatga
    70201 taaaagcacg ctcttcgatt tctttgatca ggcgatacag ttcacgttcg ctttttctag
    70261 gagaaagtat ttcgagaact agccgctcac cttccagccc cattgcagaa gtctgtgtca
    70321 caccataacc gtgttctctc agtatacccg gcaaactttt atcttctgcg ctatttgctg
    70381 gcaaaatcgt tgtgaccatg atgtatccta atgccaattt gtcttcaatc aaaattccga
    70441 cactgatacc tactgcgtaa cctaacgcat agacaaataa attcagtgga ttatctaaac
    70501 gatctaaaac aagggataag ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg
    70561 gagcaatcag tcgatagcct ttcattgtca acatgaaacg taacgtgttc agagtgatat
    70621 aagcaaaatt aatgaggaag atcatcccca acatttttgg atcaatcatt tgtaaaactc
    70681 ctttcattta tataacaaca atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt
    70741 atacatacaa aaaaatgata aaatgattat aatagaatta taaagatagg ttaggtgatt
    70801 aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg tgctaagtct ccttctcagt
    70861 atatacttag gtgtctattc gattcaacag ccaattgagg tagagaacat tgtttttcag
    70921 atgtcgctga cagatggttt aattttaagc gtttttttct ttttgatact gtttattggt
    70981 aatattttcg gtagcgttgc cagttttttg aatttttcta tttatccact cctttcgcta
    71041 gcattaggag tggtgggact ttgttctctg tgcttatttc ccgagccatt cagtcctgca
    71101 tttatattat ttggtacact gaacctgttc cagacaactg ttggagcatg gttgctatgg
    71161 cgatcaggaa atttaatgaa aataggtgag taacaattga atccaattat attgaaacgt
    71221 tctgtccatc atattccagt gttagaagtt gttctgaaag aattaagaga ggaacgtatt
    71281 cccgttgtca tctattatca cggatggcag acaagtaagg agttagtttt gacgcaagga
    71341 agaaaaattg cgcagcaggg cattcgtgtg atcctgccag atgcgatgaa tcatggggag
    71401 cgcaaacagc cggtttcttc tattccatcc tatacatttt gggatagtat ctacggaaat
    71461 ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa gaggtcttct acatgatcag
    71521 cttgcagttg gcggggtttc gatgggggga atgacaacat gcgccttgtt ggctaaacat
    71581 cctcatatag tagcgggcgt gtgtttgatg ggttcccctg cacctttgga atatggaaaa
    71641 gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac caaaagacta ttttgatttg
    71701 atttcatggg ttaagagcta tgatttgtca ttgcagccgg aaaagttagc tggacgtcct
    71761 ctgttctttt ggcatggaca agctgacgaa aaaatccctt atatacaaac ggaacgattt
    71821 gtagaaaaaa atcggcataa gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca
    71881 cagcgacata tggtacaaat accactaatg gaagaagctt ccactttttt agcaaaaaat
    71941 ttgataaaaa agtataaata attgaaacgg aaaatcactc atgacgatag aggattttcc
    72001 gttttttatt ttgggctata ataaactctg ttaggaaaat ttggtataat tgatagtaag
    72061 aatgacaaca tgttagaaag gaagagagca tgcccacttt agcagatgtt gcaaaacgtg
    72121 ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca tccggaacag gtgactgacg
    72181 aattgaaaga gttagttttc tcagcaatgg ctgaactgga ttaccgtcca aatattgctg
    72241 caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt atttatactg gaagaaatcg
    72301 acacaactga gccctattac atgaatttgc tgatgggaat cgctaaacgg atcggtaaag
    72361 cgcattattc tctccaatta gttacaaatg atgccttcga cgtcggttct tgtgatggat
    72421 acatcatcac aggtgtcaga aaaaaagact tcgattggat cgaccgtttg gaaaaaccag
    72481 ttgtcttgtt tggggaaaat tctgaaggat atgattatgt agacagcgat aatgaatatg
    72541 gtacagcaaa agcaacgcaa tatgccttag aaagagatta tcaaacgatt atttatatcg
    72601 gtatcgaagt ggacgagcct tttgaaattt caagggaaac tggttattta tctgtgatga
    72661 aggaagttga tcttcctagt aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa
    72721 gatatatgac agaaaactgg gcagatttta cagataaaac ttgttttgta tgtagttcag
    72781 atcggctagc actaggagca gtaagaggga ttataaatgc tggaggacac ttgcccgaag
    72841 aatttggtgt gattggattt gatggggtat ttttagacca aatcagttca cctaaattga
    72901 caacgatcaa gcaagatatt atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa
    72961 aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca
    73021 gagagacaac gaattaagtc ttattactat atatatattt atgaaattca tcgctatttt
    73081 cttaaaataa tcagcgcatt gctactgaaa acaatgaatc tgtgctatta ttgagaaaag
    73141 ttccattttt gagaaggatg gtttgaatga aagaaatcag acgaaaacat ttccgttttc
    73201 ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga caagaagcga gtattatttg
    73261 acggacaaga agatcttttg actgaacaga atcagtcccc gtttgaacag atgaaagaat
    73321 ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat
    73381 tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa aggaaaatta ccggattata
    73441 ccggccatta ccaatcagaa atcacatcga caggacgaaa aaaacttttt ggtgattcct
    73501 ctcgttctgt cagacaaagt gaaacaagga cagagtccaa gcatactaca cgaaacgcat
    73561 ctgttgacac gaaggtgcgg gagcgttctt attttgttcc taaatatgta cccgcatcga
    73621 ttatcccaga ccctagagaa agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa
    73681 aaacggatga ctcttatctg ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa
    73741 atggtgagcc gtctgtacga acattcaatc gaacaaaaac gttagaggaa acaaagcagt
    73801 cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga acgctcatta caaggattga
    73861 tcgatgatca aggaaactct ttgggagaaa atagctattt taaataagtg atatacaaat
    73921 ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt tccttatttt atactctatc
    73981 cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa gcgaattttt tctcccaata
    74041 agtaagaaaa taatggcaaa ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat
    74101 agtttatttt tttataaaga agattttact gaataatatc attttagttt acataatgat
    74161 atttctgtaa attaaataaa gtagtgaaaa ataagaatca gtgctctaac tgttcaagag
    74221 gatcaagtgg ttcaaatgtt tctgtagtct gttttgcttc caattggggc aaaacagatc
    74281 caatttccag atacttcact ttattcaatg tataagatag aatagaacag ctaagtaatg
    74341 tatacagaaa atgtgtgaga tccaaataaa gcaaggagac aactaaaata ccaaaagtaa
    74401 tcaagaaaag acttttgcca atcttaatat gaaaatattt ttcaatggct tgataaatga
    74461 tatctgttcc gcctgtgctt ccgccgaatc gaaagacgag accgcaacca atgccagaaa
    74521 gaatacctgt catcaaacta ttgatcagta gactatctac agcaaaatgt atatgaaggc
    74581 tctcccataa atgtaaaaag aaaactagcg caaatgttcc gacgatcgtc cggagaaaag
    74641 cgtgttttcc aaaaagaaac aatgagataa gcaataaagg taaattaaaa agcagtgaag
    74701 tgacagaagg agcgaatcca aaaagattat gcaaaagcaa agcaatacca gtaaccccac
    74761 catctgccag cttagatgga atattgattc ctgtcacagc taatccgtag attcctgcgc
    74821 ctaaaatgat tccgcacaat tctgtaaata aatttaattt tttcatttct agcctctttt
    74881 cttttgaaaa ttttcagcaa cttctttatt atactgactt atactcttac ttcaagagga
    74941 aaatgaaaaa aaattatttt tggctgtttc tttttattta tcaaataagg agtggaaaaa
    75001 tgcgagcagc agacttattg acaattctta caaaatcacc caaaaaaaca gagtatcaag
    75061 gagttttttt ggctcaagaa caaaaactgg tgcctgtgac aatggtccag ataaataaag
    75121 caaatcaatt tatcttttta tttgaacacg gcaaagcacc acttgctatg aaagacatct
    75181 ttcttttttt gatgaagaac cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca
    75241 ttcctcttta tggagtgaaa gagatagaaa ataaagtggt gatataagta aaaataagaa
    75301 aacaagacgt ttatgatgtc aaaacaacca ttttttacat ttttggcaaa aaagtgtcat
    75361 gatacagtat aatattcgtt gaacaggcca ataaaggtgt tctcccccac gagatttcta
    75421 ctttattggc tgtttctttt tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg
    75481 attccaagtg acaatttcgt atagattttt atggtattcg tagtgaaaaa acgagaacca
    75541 aaaagttgat ccgactttgt tgcttggatt ctttttttgt cagcatattt agaggtattt
    75601 ttttcttttt tttgttcgtg ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga
    75661 aagaaatgtt tatacttaaa gaataacaga aaggaaggtc gcatgaatag ttttatacaa
    75721 acattaagtg aaagaaaagg cgagttgctg gaagctgcat ttcaacatct gtccatttct
    75781 ctcatttctt tgctgattgc agcactgatt gctattcctt tagcgatttg ggcagccaat
    75841 cataaaaaaa tggcagaagt cttattgcag atcacgggtg tgctccaaac gattccttca
    75901 cttgccttat taggtttact tatcccattt gtagggatcg gaactgttcc agcattgatt
    75961 gcacttgtta tttacgcttt gcttccgatt ttccaaaaca cgtatattgg tctagctgaa
    76021 attgacccgt ctattgaaga agcagcggta gcatttggta tgtccagaat gagacgcttg
    76081 ctgaaagtag agcttcccat cgcattacct gtcattatct caggtatccg aactgcctta
    76141 gttttaatca tcggtacggc tactttggca gcattgatcg gagcaggtgg attagggaca
    76201 tttattttgt taggtattga ccgaaataca cctgttttga cattgattgg tgcgatcagt
    76261 tctgccttgc ttgcaattat attcagcgga ctgattcgct ggttgcagca taagaaacct
    76321 cgatattcca tcattacttt agcagcagtg gtgtgtttat ttggcggcgt ttctttatat
    76381 cagtctgcct tgtttaaaaa tgaaaccatc acgattgctg gaaaattagg agcggaacct
    76441 gaaatcctga tccaaatgta taaagaattg attgaagaca acacaaaaac agaagttgaa
    76501 ttaaaaccga attttggcaa gacgagtttt ttgttcaatg cactggatca aggtcaaatc
    76561 gatatctatc ctgagttcac aggtactgtc ctagaaagct tggtgaaggt acctgaagca
    76621 atgaaaagtg ctgatttgac aaaagaaaaa acatatgacg aagcagaaga attattgaat
    76681 aagcaattcg atatgacatt attaaaacca atggcttacc aaaatacgta tgccttggca
    76741 gtcaagagat cttttgcgaa cgaacatcaa cttaaaaaga tttccgattt ggcagctatc
    76801 tctgatcaga tcaaagcggg attcacactg gaatttattg atcgttcaga tggatataaa
    76861 ggaatccaag aagcatatgg cttgaatttt tcaagtgtac aaagcatgga gccaagtttg
    76921 cgttaccaag caatcaataa tggagatgtc aatattgtgg atgcctattc cacagatagc
    76981 cagctcaagg agtacgatct agtgactcta gaagatgatc gagggttatt cccagcttat
    77041 caaggcgcac cgctgatgaa aaaagatttt gctaaagaac atcctgaaat cgttaaagca
    77101 ctgaataaat taagcggaaa aataacagag gatcaaatga ttgatatgaa ttaccaagtc
    77161 aatgtcgaaa agaaaaagcc gacagaagta gcacgaatct tcctgaaaaa tgaaggatta
    77221 gtaaaggagg aagatcagtg actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg
    77281 atggaaagta tgtcatcgat gacctaaatc tggcaatcaa aaaaggagaa atctttgttt
    77341 tggttggtcc ttccggtagt ggaaaaacaa ctactttgaa aatgatcaat ggattatcta
    77401 aaccatcagc aggagacatc tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga
    77461 aaatgcggtg gaatatggga tatgtgctgc agcaaatcgc tttgttcccg accatgacag
    77521 tcaagcaaaa tattgaagtc atccctgaaa tgttaggttg ggaaaagcag aaaagagcag
    77581 accgtgtaga tgaacttctt caaaaagttg gcttatctcc tgatatttat cgagatcgta
    77641 tgcctagaga gttgtctgga ggagaacagc aacggatcgg gatcatccga gctattgctg
    77701 caagcccaga tgtgattttg atggacgagc catttagcgc attagatccg atttcaagaa
    77761 actcattaca agaattggtt ttgtccttac acgaagaatt gggcacgacc atcgttttcg
    77821 ttacgcacaa tatggaagaa gcaatcaagc taggtgatcg tattgcattt atgaaggatg
    77881 gagaaattat ccaatgtgac acaccagagc agttattgat gaatcctaaa aacgactacg
    77941 ttcggcattt ctttgatgaa ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg
    78001 tcaatggata tttcttaaat gaaataccag aaaatgctcg tcaacaagtt tgctttgata
    78061 cgcctatgaa agaagtatat tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa
    78121 agcaaagaag tattgggtcg ctgacaagta aagaaatctt tgctttttta agtcgagagg
    78181 aggaaggaaa tgaaaacata tgatgcaatc atcatcggtt caggtgtgag cggcttaagt
    78241 gctgcttatg gattgaaaga agcaggtaaa acagtactag tggtcgaaga agatctatgg
    78301 ggcgggactt gtcctaaccg tggatgtgat cctaaaaaag tcttgttaag tgcagtagag
    78361 gcacgtaatc gggtaaaaca attgagtgga aaaggtttta acgagatccc aactgccaac
    78421 tgggaagaac ttcaaaaatt caaacgaaca tttacggatc cagtcccaga aagccgaaaa
    78481 aaacaattag cagaagctga aatcgatcat ctttcaggaa ccgctcgttt tttggatgat
    78541 tcatctattg aagtcaacga agaagtcttc catgcggatt acttagtttt ggcaaccggg
    78601 caaagaccta cgatcttacc agtggaggga aaggaatact tgaaaactag tgcagacttt
    78661 ttatcattgc ctgtactgcc taaagaaatc atttttatcg gtggtggtta tattgctttt
    78721 gagctagcta cgattgccaa tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa
    78781 cggccattaa aagaatttga ggcgtctttg gtagaggaag ccgtccatca aatggaagca
    78841 tctggcattc agtttgcttt tggtgttgaa acccaaaaga tcatttcaga aggtactcgc
    78901 tatcgtttag tcggaaaaga gacggaatta gtagcagata tgattttttg tgcgactggg
    78961 agacagccga atacagaatc tttagcactt gagcaagcga atatcgtctt tgataaacac
    79021 ggaattgcag tcaatgatta tctgcaaacg agcaatccaa aaatttttgc gtgtggagat
    79081 attgtttcaa ggaaaacccc aaaattgaca ccagtcgcca cttttgaagg aaattatgta
    79141 gctaagcgga taacggatgc aacaagcgaa cctatcaaat atccgattat acctacgatc
    79201 gtctacgcca gtcctaaact tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca
    79261 gatcaagtgg tagagatgga tcttaccagt tggttcacct atcatcgtgt caatgaacca
    79321 gttgctaagg ctgaattgac ctttgaccag caaaattatt tgattggtgc tgcagtgatt
    79381 agcgagcaag cagacgaatt gatcgatgat ttgacactcg tcatcaatca aaaattgact
    79441 aaaaaagagt tggatagcta tatcatgggg tatccgacat tggcaagtga tctctcctat
    79501 ttattgaaat aatcagcatg ataactaaag cgcttgttgc tttttccttc ttgtgctaca
    79561 ctttaaaaaa tgaatacctg tttctgggga tgtcgtagac gacatgagag taagttgtga
    79621 acttagaccc attgaacctg tcagttaata ctggtgaagg gagaaacata gccgacgctt
    79681 gaagcgggtt actatgtcgt gcccatttca agcgtttttt gttattcata ttagaagaaa
    79741 ggaggtaaca aaatgaagaa aaatctttat ccagcagtca gtatggcatt cattttaatt
    79801 atttggcaga ttgttgtcat agtttggaaa atacctgcct ttattttacc cggacctgct
    79861 gctgtatttt ctgcattagc ggcagatgca caagtattac tttcccattc tgtcacgaca
    79921 ttaaatgaag cagtgcttgg tttggtaata gctagcgttc tttcttttat tactgccttg
    79981 gcaatggatt attggaagtt tttagaaatg tctttttatc cgttactagt gatttcacaa
    80041 atgttgccaa tcatggtgtt agggccatta cttaccctat ggtttggttt cggtatgctt
    80101 ccaaaagtga tccttgttgt cttgataagc tattttccga tcgtagtttc tttttcggat
    80161 acattgagga aagtatcaaa agagcaaatc atctttttga aaacgatggg ggcagataca
    80221 gggaagattt atcgaatata taaaatacca gcgggtttat ccggcttctt ttccggttta
    80281 aaagtggctg ctacttattg tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa
    80341 gcaggcttag ggtattacat gatacgcgta aaaaacagct accagatcga taaagttttt
    80401 gctgcgatca tttgtgtcat tctattgagt ttattgctga atggcgcttg ttcattattg
    80461 aaaaaagggt attatcgtat gctttatatt ttttaagaga aggggatcaa atgaaaaaat
    80521 tatggctttt attaccgcta ctgttgcttt tatccgcttg tggcactgca aaagaaacaa
    80581 gtagcaaaca agaaataaag gatttgaaaa aagtgacact tgttttggat tatgtaccga
    80641 atactaatca tactggcatc tacctagcaa aagaaaaagg ctattataaa gaggctggtt
    80701 taaatgtcca aatcatcgaa ccaggagata attcgacaag tattggtcta gtaggtgcgg
    80761 acaaagcaca atttggtgta agttatcaag aagatgtcac ttatgctcac gcagatggac
    80821 agaatatacc agtcaaagca attgctacag tgatcaaaca taatacttct ggattcgcca
    80881 ccctatcaga cagtaacatc cattcaccaa aagattttga aggaaaaaca tatgcaggtt
    80941 ggcaaagtcc aagtgaagaa gctgtcttaa aagctgtgat ggaaaaagac ggcggggact
    81001 tttcaaaact gacaatggta ggaagcaatg gagaagggcc agaaagctta ggaaaatcct
    81061 cagatatcca atggtacttt gaaggttggg atatgatcaa agcaaaagaa gcgggtattg
    81121 aactaaacta tatcccattg aaagaattag atgaacggtt agattactat actccggtaa
    81181 ttattacgaa tgatcaactg atcaaaagtg atccagaatt ggtacagtct tttatggatg
    81241 ccacgaaaaa aggctatcag gaagctatta aggatccaaa tgacagtgct aaactgttgc
    81301 aaaaatatgc aaaagaaaat gatcggacgt ttttagaaga atcacaagca ttcttgtcaa
    81361 aaaattatac ggatgatcct gagaattggg gattgatgga agaaaaagtt tggcgcaatt
    81421 atacagcttt tatgcaggaa aacggattga tcaagcaaga tgtaccttcg caagaactat
    81481 tcactaatca gtttataaaa taggagtgat agatatgtca atccaaatca gccacttgaa
    81541 tgtagtcatt gaaaacctga aaatattaga agacatccat ttcactatcc ctaaagatac
    81601 ctttacatcg attgtcgcgc caagcggagc cggtaagtca acattgctga aaactttaac
    81661 aggagtccag cctatgactt ctggaacgat caaagtagac gatcaaaaag taactggtct
    81721 ccatacagca tttagttata tgccgcaaga agatatgtta cttccttggc tgagcgtcta
    81781 tcaaaacgtt acgttatatc aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag
    81841 ggattatctg gatatctttg ggttaaacgg ctatgaacat tttttgccgg aacaattatc
    81901 tggcgggatg aagcagcgaa cggccttgtt acgaacaatc atgaatcctt cttcttactt
    81961 gcttcttgat gaaccatttg gagcgttaga tgctatgacc agagggcaga tgcaagactg
    82021 gatgttgaag ctgcctaaaa aagcaaagcg gactacttta cttgtgacgc atgatatcga
    82081 agaagcaatt tatttatcag acaggattct tgtgctttcc gcccgtccgg cacatgtgat
    82141 tgctgagatt caagtgcctg aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga
    82201 gttgaagcaa acgatttatc agttattggc aggtgaatta gatgttaagt gatgctcatt
    82261 gtcatttaga tgggagtcag agtttagcac tccttcagca ggaacacacg attttgacga
    82321 tcatcaattg tgattcgcca gaagaatgga aggaaaacag acagcttgca gcaagtaaga
    82381 ctcaagcctt aagttacggt atccatcctt ggaaagcaga tagctatacg tttgaacaag
    82441 ttgagccttt tttgaaaaaa gcaaggatca tcggtgaaat agggttagat aatatttgga
    82501 cgaatgtacc gatgactaca caaaaaaaag tatttgagcg acagcttgct tttgcagcca
    82561 taaatgagaa acctgttgta ctgcacacaa aaggatgcga aaaagagatc ttaaattata
    82621 tccaagctta ccctaatcac tatttgatcc attggtattc ttctttggac tatcaacaag
    82681 actatattga tttaggctgt tatttcagca ttggtcttga tttgaaaaag aatccagcag
    82741 tctggcaact agctaaagca gtcccaatcg accacttact tattgaaacg gatggactac
    82801 atgcagcaga gtgggcttta ggggggattt tttcagaaaa agattatgtc tcactgcttg
    82861 aagaacagtt aaagacaatc gctgagatca aagagctatc agttgaagaa gttaaaaaaa
    82921 atacttatca aaacttggaa cgtttcatta gattaggtaa ataaaaaaga gatgtgacac
    82981 aactagtgtc gcacctcttt ttggctttct ttgtataaaa gtaaactgtt tgattaataa
    83041 atgaatctta gtaccaaccg tttgcttccc agaatgctt
//
LOCUS       DACGLH010000235        39380 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.020, whole genome shotgun sequence.
ACCESSION   DACGLH010000235 DACGLH010000000
VERSION     DACGLH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.020"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            111..590
                     /locus_tag="IXT21_003160"
                     /pseudo
     CDS             111..590
                     /locus_tag="IXT21_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            1437..2012
                     /locus_tag="IXT21_003161"
     CDS             1437..2012
                     /locus_tag="IXT21_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ4870478.1"
                     /translation="MKFTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            2012..2452
                     /locus_tag="IXT21_003162"
     CDS             2012..2452
                     /locus_tag="IXT21_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ4870479.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            2468..3169
                     /locus_tag="IXT21_003163"
     CDS             2468..3169
                     /locus_tag="IXT21_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4870480.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(3657..4082)
                     /locus_tag="IXT21_003164"
     CDS             complement(3657..4082)
                     /locus_tag="IXT21_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870481.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            4447..5646
                     /locus_tag="IXT21_003165"
     CDS             4447..5646
                     /locus_tag="IXT21_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4870482.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            5668..5880
                     /locus_tag="IXT21_003166"
     CDS             5668..5880
                     /locus_tag="IXT21_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ4870483.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            5955..6431
                     /locus_tag="IXT21_003167"
     CDS             5955..6431
                     /locus_tag="IXT21_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ4870484.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            6428..8236
                     /locus_tag="IXT21_003168"
     CDS             6428..8236
                     /locus_tag="IXT21_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ4870485.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8313..8528
                     /locus_tag="IXT21_003169"
     CDS             8313..8528
                     /locus_tag="IXT21_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAQ4870486.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            8620..9483
                     /locus_tag="IXT21_003170"
     CDS             8620..9483
                     /locus_tag="IXT21_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870487.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            9514..10056
                     /locus_tag="IXT21_003171"
     CDS             9514..10056
                     /locus_tag="IXT21_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAQ4870488.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            10070..10492
                     /locus_tag="IXT21_003172"
     CDS             10070..10492
                     /locus_tag="IXT21_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ4870489.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            10422..12821
                     /locus_tag="IXT21_003173"
     CDS             10422..12821
                     /locus_tag="IXT21_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ4870490.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            12853..14844
                     /locus_tag="IXT21_003174"
     CDS             12853..14844
                     /locus_tag="IXT21_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ4870491.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            14867..15118
                     /locus_tag="IXT21_003175"
     CDS             14867..15118
                     /locus_tag="IXT21_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAQ4870492.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            15108..16337
                     /locus_tag="IXT21_003176"
     CDS             15108..16337
                     /locus_tag="IXT21_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAQ4870493.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            16334..18415
                     /gene="topB"
                     /locus_tag="IXT21_003177"
     CDS             16334..18415
                     /gene="topB"
                     /locus_tag="IXT21_003177"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4870494.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            18564..22484
                     /locus_tag="IXT21_003178"
     CDS             18564..22484
                     /locus_tag="IXT21_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAQ4870495.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITKIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            22485..23429
                     /locus_tag="IXT21_003179"
     CDS             22485..23429
                     /locus_tag="IXT21_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870496.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            23433..23636
                     /locus_tag="IXT21_003180"
     CDS             23433..23636
                     /locus_tag="IXT21_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870497.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(23936..24172)
                     /locus_tag="IXT21_003181"
     CDS             complement(23936..24172)
                     /locus_tag="IXT21_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4870498.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(24355..24726)
                     /locus_tag="IXT21_003182"
     CDS             complement(24355..24726)
                     /locus_tag="IXT21_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870499.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(24723..26051)
                     /locus_tag="IXT21_003183"
     CDS             complement(24723..26051)
                     /locus_tag="IXT21_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ4870500.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(26012..26341)
                     /locus_tag="IXT21_003184"
     CDS             complement(26012..26341)
                     /locus_tag="IXT21_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ4870501.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(26599..26970)
                     /locus_tag="IXT21_003185"
     CDS             complement(26599..26970)
                     /locus_tag="IXT21_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4870502.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            27115..27300
                     /locus_tag="IXT21_003186"
     CDS             27115..27300
                     /locus_tag="IXT21_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4870503.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            28300..29106
                     /locus_tag="IXT21_003187"
     CDS             28300..29106
                     /locus_tag="IXT21_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAQ4870504.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            29124..29951
                     /gene="vanW"
                     /locus_tag="IXT21_003188"
     CDS             29124..29951
                     /gene="vanW"
                     /locus_tag="IXT21_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAQ4870505.1"
                     /translation="MDRKRLTQRFPFPLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            29948..30919
                     /locus_tag="IXT21_003189"
     CDS             29948..30919
                     /locus_tag="IXT21_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ4870506.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            30912..31940
                     /gene="vanB"
                     /locus_tag="IXT21_003190"
     CDS             30912..31940
                     /gene="vanB"
                     /locus_tag="IXT21_003190"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ4870507.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAVCMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            31946..32554
                     /locus_tag="IXT21_003191"
     CDS             31946..32554
                     /locus_tag="IXT21_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ4870508.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            33142..33573
                     /locus_tag="IXT21_003192"
     CDS             33142..33573
                     /locus_tag="IXT21_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4870509.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            33580..33813
                     /locus_tag="IXT21_003193"
     CDS             33580..33813
                     /locus_tag="IXT21_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4870510.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(33735..33977)
                     /locus_tag="IXT21_003194"
     CDS             complement(33735..33977)
                     /locus_tag="IXT21_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870511.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            34234..34437
                     /locus_tag="IXT21_003195"
     CDS             34234..34437
                     /locus_tag="IXT21_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ4870512.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            34521..35714
                     /locus_tag="IXT21_003196"
     CDS             34521..35714
                     /locus_tag="IXT21_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4870513.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            36154..37125
                     /locus_tag="IXT21_003197"
     CDS             36154..37125
                     /locus_tag="IXT21_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4870514.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            37157..38554
                     /locus_tag="IXT21_003198"
     CDS             37157..38554
                     /locus_tag="IXT21_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4870515.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            38713..39336
                     /locus_tag="IXT21_003199"
     CDS             38713..39336
                     /locus_tag="IXT21_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870516.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        10669 a         9438 c        10266 g         9007 t
ORIGIN      
        1 cagccaaaca aaaatagctt ctcatatttt tcgtttggtt aggcttatta ccgcagatac
       61 acggctgttt ctggattacc atttattctg gataaggggg tgtggcacga ttttcagaag
      121 gaatctatga accaatcact gtattgattt tagcttcgat ggtaacccca ttcggcttga
      181 cgatcgctta tttcttaggg aaaatcattc gtaaaaatat attcaatcgt caagaaatcg
      241 atacattgaa gaccgctttt cctatgggga tctgtcagat cacagaggga tgtttcccta
      301 tcgtattgaa cgacttggca cgcaatgtca tagcaacggg agtcggtggg gcagtaggtg
      361 gcggattaag catgttttgg ggcgcagata gccgtattcc ggcatcggga atgtttgcag
      421 tcgctacgat gaccagacca tgggcattca ttggtgcatt gttagctggt tcttttgtca
      481 caggaataac cattttatta ttgaaaaaac cagtagatcc gaatgcagaa atcatccaag
      541 aagaaaaaga ggaagaagac atatcttggg atgatttgac aattagttag gagaggcaga
      601 atggattaca agaaaaaagt acaatagatg taccttgagg caggaatggt atgaaagaat
      661 cagagttaga aagaatcgaa tatacagatt ttggtttagg agcgatcgaa actgtccaga
      721 gcatcggcac cctccttgaa atgacgatga aggaaaaatg agaacctttc gttgtcgaaa
      781 aggtctggta taccttatgt cgaaggaaag gaaacaaaaa aactttcctg tactattcca
      841 aaaaggaaag aaagttatta tacagtaaaa aaagaaatta ttcttcatgc tagagatcag
      901 tacacgttcg agcccaatat caaacactag ttccaggcag gcgcaacagg tgcggttatc
      961 actgaatttt cttccagcag tgacgattca agtgatattt ttacaaatcc agctatttat
     1021 cgttaacaaa aagattttta ggataagctg atgaacaaaa atgatcaaat tttgatcgtt
     1081 tagttcgtcg gcttttttat ttagagaaac atcatttatc agtgaaacat aacacgagaa
     1141 caaaatgatt ttttcgttaa taaaagaaac aagtatttgt tgtttaaaat aatacacgtt
     1201 attggcatat aggacgaaat cccagtatct tctataagta agtcatttca tatcgatgaa
     1261 aacacgttat ttatttttgt tttatctata aaatagctga aaaattattt tttctaaata
     1321 ctatatatag tggtgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat
     1381 tttaaaaaaa cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga
     1441 aatttactgt tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc
     1501 aaacgttagg aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact
     1561 ccgggattat gggagtgatt agtaaaacag tactggaaaa taatggaaaa gtcacaggta
     1621 tctatccgaa tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc
     1681 ctacagaaac gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg
     1741 ttttccctgg aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa
     1801 tcggtctgac gcctgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct
     1861 tgcaacaatt attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc
     1921 aagtaatctt ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta
     1981 aaaatcagtt attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt
     2041 tttttcagag gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa
     2101 aacagtcaca agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag
     2161 cgctggatgg gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt
     2221 tgttgttgct atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt
     2281 aggtgtggca actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac
     2341 gaacttgatg attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga
     2401 atatgatttt gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa
     2461 gggttgtatg tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac
     2521 ggatatgctt gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga
     2581 agtattgccg ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt
     2641 tttgaacaag cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga
     2701 ccgatccatc tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac
     2761 ggatatcgaa gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga
     2821 tgttgctcca aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat
     2881 gctagaacgt atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt
     2941 atatacctat tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt
     3001 atgtcctaaa atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc
     3061 aaaaaaggtg atccatcaga tccaagaaaa actagctgaa ttggaaggaa agcctgctgg
     3121 aaaatatttt acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaaa
     3181 caaacgacgg gaaagaaaaa ttactacgtg atgtagaata agcctttcta tcgtttggtt
     3241 attttatatt caaatatttg aaataaataa atctttactc tttgatttgt aaactgaaat
     3301 gatccactgt ttgttttact gatgaagctt gatcaaagga agaagctgag tttgaagttc
     3361 ataaaaatca gccatagtga gttttgcttg gatttgttta tcttcttcta ctttttacat
     3421 aattttgaac aggataagat tcaaatgttt taaatgaatc tttgactagt ttgtgacagt
     3481 tgtcgcatat gctcaagctt ccttggtcat taaattctgt atggatattt ttgttgttat
     3541 tacatgtaca ataatcagga ctatcgatca tccatttcgt ctcctttaca atcatcttgc
     3601 ttctttacta tagcagaaaa gaaggggaga aacaaaagaa gatccaacag acggcactat
     3661 agactgtaga tgttgccatc ctcatccttc aaacggtaat taccgatttt ggtaaatgtg
     3721 atggttgctt cttttttatt ttcttttcct gaaataatat attcgtcatc tttggtagta
     3781 atcttgatgt ctgtcagcac tgttttttct tcagatgatt cagaagaatc agtttcttgt
     3841 gtctgatacg tgcgtgtcca ttccattttt ttatctgtaa atgtcaactg tcccgcggat
     3901 aaatccatat ctttacgtac ttttagcgta tatacactgt tagtgatatc tgctttttcc
     3961 gcttttaaga cagaagaatc ggttgtttca tccgaggatt ggtcagagcc gccaaacaaa
     4021 ttacagccag tcaatgtcaa aagtcccgca gacaaaataa atagtacgat taatttcttc
     4081 aaaatacttc ccccatttga taggataaag aaattatacc attggaaagt aaaacttcaa
     4141 acggatggaa gagaaaaacg cacaaaacac ccacaaatag agtaaatttt atttgaaaat
     4201 tttaggttat cgctgaaagc cccggaaata cgggcatttc gcaggatatg agaactcaat
     4261 ttactaccaa tttactactt tcggattgag ccgcatatat gctgaaccat actgctaata
     4321 cccgccaccc caaatacggc acatcataaa ttgaatacga caccgtaaac gcggcgttcc
     4381 tctttcccgt catgggagaa caggacgccg cttttttatg cccaaataga gaaaggaggc
     4441 gacgccatgc tatttacttc cggccctgac cgggcgtttg aacggctgat gcaggaaaag
     4501 cccggtttcg atcactacga cggcggcggg gcggacgcgc cggaggattg cggcacctgc
     4561 cgattttacc gcccggattg gaaatatcaa ttctgccagt ttgcagagtg tccctatcag
     4621 ccgggcaagc tgaccgacta tgcagcggtc aaattcacag tgaaaggagc gaacaacgct
     4681 atggcagttt tccgtgtgga aaagaacaaa ggctatacgg tgatgtcaaa tcaccacctg
     4741 cggaataaga acctgacctt aaaggccaag ggcctgctgt cgcagatgtt gtccttgccg
     4801 gaggattggg actataccct aaaaggactg tcctatatca accgggaaag catcgacgcg
     4861 atcaggacag cggtatggga gcttgaaaaa gctggatata tccggcgtga gcagggccgt
     4921 gacgcaaaag gcaaaatggc cgatatggtc tataccatct acgaacagcc ggtattggaa
     4981 aacccggtat tggaaaatcc aacatcggat aacccggtgt tggaaaatcc gacatcggat
     5041 aatccggcgt cggaaaatcc aacgcaatta aataaagaag tacaaagaac tgacttatca
     5101 aaaaaagaaa aaataattac agatggacaa agtacccatt ccattcctat cccatccccg
     5161 acccctgccc ctatggaggg cgaggcagcg gctacgccgc cggaacggaa aaggacagga
     5221 aaggatgagg ctgtacggat ttatcgggaa atcattttgg agaacatcga gtatgcctat
     5281 ctcattcagg acaggagcat tgaccgtgaa cagcttgacg aaatcgtaga cctgatgctg
     5341 gaaaccgtct gcaccgcccg aaagacaatc cgtgtcgccg gggacgacta ccctgccgag
     5401 ctggtgaaat ccaagtacat gaagctgacc ggcgaacata tccgctttgt ccttgactgt
     5461 ctgcgggaga acacaacgaa agtccgcaat atcaaacaat acctgcgggc tatgctgttc
     5521 aacgccccca gcaccatttc caactactac acggcccttg tcgcgcacga catggcacag
     5581 cccgattggg gaaagcctaa atccggcctg ccggactatt cctgcggcga gggcgaaagc
     5641 ctgtaacgag aaaaggagga cgaccctatg aagcagggcg cattgatttt tgacgagcga
     5701 acagaccgtt acgacatccg ctttgatctg gccgactatt acggcggcct gcattgcggg
     5761 gagtgtttcg acgtgatgat cggcggcaaa tggaggccca cccgcattga aatggccgaa
     5821 aactggtatc ttgtcggcat ccgggccgac gacctttccg gcctgcgggt gcggatttga
     5881 cctatatcta cctgcggcgt ctggtggttc cgccttagac gccgcttttg actttctgac
     5941 aaggagggat gcgattgcag gaagaagtta ctcaaaagac cattgccctg tccatgaaaa
     6001 cgggcaagct cacggctcaa gtgctgcaag cggcgctgaa aaagtacctg caacaccggg
     6061 ccaagggcaa aaacacgctg caccacggcc agcagagcct aaagcagctt aaaaagcatg
     6121 gggctgatct ctccaacatt gagatcaccg aggccaacat tggagcgttc aagccatgcg
     6181 ccaagaaata cggcgtggat tttaccttac gaaaagataa gaccacccag ccgccccact
     6241 acgttgtgat tttcaaggcc aaggatgcag ataaccttga tctggcgttt cgggagttta
     6301 ccgccaagac gctttccaag gagcagcggc cctctatccg caaggttttg tccgctgtga
     6361 agcaaaaggc ggcggcccag accaagcagc gggccaagga gaaaatcaag gaaagggggc
     6421 tggaactgtg aagcctgacg taaagaaatt gctgatcctg aacctcccgt atctgatttt
     6481 tgtgtacctg tttgcgaaat gcggggaggc gtaccggctg gccgctggtg ctgacctgtc
     6541 ggggaagctc ctgcattttg cggatggttt tgcggcggct tttgccaacc cgctgccaag
     6601 cctgcacctg tttgacctgt gtatcggtat cgccggtgcc gtgatcgtgc ggctggtggt
     6661 gtactgcaag agcaagaacg tgaagaaata ccgcaaaggc atggagtatg gcagcgcccg
     6721 ctgggggacg gccaaagaca tagcgcctta catcgacccc aagtttgaaa ataatatcct
     6781 gctcacccaa acggaacggc ttaccatgac cggcaggccc aaagacccca agacagcgcg
     6841 gaataaaaac gtgctggtga tcggtggcag cggttcaggg aaaacgagat ttttcgtgaa
     6901 acccaacctg atgcaatgct ttccgaccac ggactatccg acttcgtttg tggtgacaga
     6961 tccgaaaggc actttggttc tggaaaccgg caggatgttc cagcaggcgg gctaccgcat
     7021 taaaatcctg aataccatta acttttccaa gtccatgaag tacaacccct ttgtctacat
     7081 ccattcggaa aaggacgtgc taaagctggt aaataccctg atttccaaca ccaagggcga
     7141 gggggaaaag tcagcggagg atttttgggt gaagtcggaa cggttgtttt attcggccct
     7201 gatcggctat atctggtacg aggccccagc ggaggaaatg aacttctcaa cgctgctgga
     7261 aatgataaac gccagtgaag cccgcgagga cgacccggag tttcaaagcc ccgtcgatct
     7321 catgtttgag cgtttggagc agaaagaccc cgaccatttt gcggtgcgcc agtacaagaa
     7381 attcctgctg tccgcaggca agacccgttc ttccatcctg atttcatgcg gtgccagact
     7441 tgcgcccttt gacatccggg aggtacgcga gctgatggag gacgacgaaa tggagctgga
     7501 caccatcggg gacgaaaaga cggtgctgta tctcattatg agcgatacgg acacgacctt
     7561 taatttcatt ctggctatgc tccaaagcca gctaatcaac ctgctttgtg accgggctga
     7621 tgatgtgtac ggcgggcggc tgcctgtcca tgtacgcctg attttggatg aatttgccaa
     7681 cattggccaa atacccaatt tcgataaact cattgccacc attcgaagcc gtgaaatatc
     7741 ggcctctatt attttgcaga gccagtcgca gctaaaagcg atttacaagg acgccgccga
     7801 gatcatttcg gataactgcg atagtgtgct tttccttagt gggcgcggca aaaatgccaa
     7861 ggagatttcc gatgcgctgg ggaaagaaac gattgacagt ttcaacacca gcgagaaccg
     7921 gggaagccaa acgtcccacg gattgaacta tcaaaaatta ggaaaggagt tgatgtccca
     7981 agacgaaatt gccgtgatgg acggcggcaa gtgtatcttg cagctacggg gtgtgcgccc
     8041 gtttttatcg gagaaattcg atattaccaa gcatccccgg tacaaatacc ttgccgacgc
     8101 cgacaagaag aatacctttg acgtggacag atttttgact atcacgcgcc ggaaacggca
     8161 gcaagtggta acgcaggatg aagtattcga cctgtatgaa atcgacctgt cggacgagga
     8221 cgccgccgct gaataaacag cggctttttt cattccaaca acaaaatcaa aaaatcatta
     8281 tgagccggaa accggcagaa agaggtaaat ttatggcatt ttttaattcc gcagttacta
     8341 ctttgcagac tatcgtgatc ggactgggcg gcgctctgtg cgtatggggc ggcatcaatc
     8401 tgttggaggg atacgggcag gacaatccgg gggcaaaatc gcagggtgtc aagcagcttg
     8461 tcgctggcgg cggcgtggcc ctgatcggtg tcactctggt tcccctgctg tccgggctgc
     8521 tgggctgatc ctggcctttg tccctgtcca aacccttaac taatcgcctc cgcgtcctcc
     8581 cggccagacc gggagggcgc gggaaaggag gtgctacccg tgattagtga aatcattgaa
     8641 gaatggataa aaggcatcct catggacggt atcacgggca acctgtccgg gctgttcgat
     8701 acggtaaatg ccaaagtcgg agaaatcgcc gccgacgtgg gacgaacccc gcaggcatgg
     8761 aacagcggta ttttcaatat gctgcacagc ctgtcggaaa ccgtgatcgt ccccatagcc
     8821 ggggccatac tcgcccttgt catgtgctat gagcttatcc agatgataac ggaaaagaac
     8881 aatatgcacg actttgacag ctccatgttc ttccgctgga tattcaaatc cgcgtttgca
     8941 atcctgatcg taacgaacac atggaacatc gtcatgggcg tattcgacgc cacccaaagc
     9001 gtcgtcaatc aaagcgccgg tgtgattatc gggcagacat ccattgattt taacaccctg
     9061 ataccagacc ttgaaagcca attggaggct atgagcgtgg gcgctctttt ggggctgtgg
     9121 ttccaaaccc ttgttgtggg cctcaccatg aatatccttt ccatctgcat tttccttgtg
     9181 acctatggcc gcatggtgga aatctatgtt gtcacgtcgc tggggcctat cccccttgcc
     9241 acaatgggca acgcggaatg gcgcggcatg ggccaagggt atctgaaatc cctgctggcg
     9301 ctgggcttcc aagccttttt aatcatggtt gtggtgggta tctacgcggt gctgatacag
     9361 aatatcggtt ccgcttccga catatccggc gcaatctggg gctgtatggg ctatacggtg
     9421 ctgctgtgct tctgcctgtt caagaccggc agtatatcaa aggccgtatt cggggcgcat
     9481 tgatcccaaa atccaagcat acacaggagg aacttgttta tttcaacgta caacatcatt
     9541 tacgctgacc cgccgtggcg gtatgactgc aaaaacgtac agggcgcagc ggaaaatcac
     9601 tattccacca tgagcattga cgagctgtgc gccctgccgg tggaacggct ggcgtccaaa
     9661 gactgcctgc tgtttttgtg ggccacattc cccatgctgc cggaagctct gcggttaatc
     9721 aaagcgtggg gcttttcatt caagaccgtg gcttttgtat ggctgaaaca gaaccgtaaa
     9781 agccttacat ggttttacgg gctgggccgc tggacaaggg gaaacgcgga aatctgcctg
     9841 ctggcaacac acggcaagcc gaagcgtcgt tctgcctctg tccatcagtt tattatcagc
     9901 ccgattgaac aacacagcaa aaagccggat gtgacccgtg aaaaaatcgt agagctggcg
     9961 ggcgatttgc cccgcgtgga gctgtttgcc agacagaaaa cccccggctg ggacgtgtgg
    10021 gggaacgagg tggaatgtga ccttatcctg acgtgaaagg aggtgccaaa tggcttatgt
    10081 aaccgtcccc aaggacttaa cccatgtgaa aagcaaggtt gtgtttgggc ttaccaaacg
    10141 gcaactgatc tgtttttccg gggcgctggt gatcggcgtc cccttatatt ttctcacccg
    10201 tgactatctt accaacagcg cggcggccct gctcatggta tttgccatgc tgccgggctt
    10261 tttgcttgcc atgtacgagc gtcacggcca gccccttgaa gtggtgatta tgcagatcat
    10321 ccagtgctgc ttcatccagc caaaggagcg tccctatcag accgacaaca cctacaccgc
    10381 ccttgtgcgg caataccaga tggaacagga ggtaaaggcc attgtccaaa aaggaaatcc
    10441 caagagaaac cgaaaaaaca aagctgaccc gcgcccagcg aaaagaaatt gacgcggtaa
    10501 tccgtaaata taagggcgac ggcaggcccc acacggcgca gcagagcatc ccctacgagg
    10561 tcatgtaccc ggacggcgtg tgccgggtat cgcccggcgt cttttccaag tgcattgagt
    10621 ttgcggacat cagctatcag cttgcccagc cggacacgca gacggctatc tttgaaaaac
    10681 tgtgcgacct gtataactat gtggacgcct ccattcatat ccagttttct ttcctcaatc
    10741 ggaaagttga cccggtgcag tacgccaaga gctttgagat cgcgccgcag ggggacgact
    10801 tcgacgacat ccgggccgag tacaccggca ttctgcaaaa gcagcttgca aacggcaaca
    10861 acggcatggt aaagaccaaa tacctgacat tcaccatcga ggccgaaagc gtgaaagcgg
    10921 cgcgagccag attgaagcgc atcggctttg acctgttagg ctatttcaag agcatggggg
    10981 ccgtagccca tgtgatggac ggctgggaac ggctgaacct gctgcatggc gtgtaccacc
    11041 cggacggcga gatattcaat tttgactgga agtggctggc cccgtccggc ctctccacca
    11101 aggattttat tgccccgtcc tccctgtgct tcggcaatgc aaagacattc ggcatgggcg
    11161 gcaaatacgg cgcggtaagt ttcctgcaaa tcctgtcccc ggagctgtcg gacgatatgc
    11221 tggccgactt cctcaatacg gaaagcggtg ttttagtcaa tctccatgtg caggccattg
    11281 aacagaccaa ggcgatcaag accatcaagc gcaaaattac cgaccttgac gctatgaaga
    11341 ttgcggagca gaaaaaggcc gtccgcagcg gttacgatat ggacattctt cccagcgacc
    11401 ttgccaccta cggcgaggac gccaagaagc tgctgacaaa gttgcagacc cgcaatgaac
    11461 ggctgttcca gttgacgttc cttgtcctca acgtggccga caccaagcaa aagctgaata
    11521 acgacgtgtt ccaagccgca ggcgtggccc aaaagcataa ctgtcccctt gtccggctgg
    11581 actaccagca ggaacagggc cttgcaagca gcctgccgct gggcgtcaat cagatcaaga
    11641 tacagcgcag ccttacgact tccagcgtgg ccgtgttcgt cccctttgtg acgcaggagc
    11701 tttttcaggg cggcgcggcc atgtattacg gcatcaacgc caagtcccgc aacatgatta
    11761 tgctggacag gaagcaggcc cgttgcccca atgccttgaa gctcggcacc cccggcagcg
    11821 gcaaatccat gagctgtaaa tccgagatcg tcagtgtgtt cttaacgacg cctgacgata
    11881 tttttatttc agacccggag gccgaatatt acccccttgt caaacggctc cacggacagg
    11941 taatccggct gtcgcctacc agcaaggact ttgtgaaccc gctggacatc aacctgaatt
    12001 attcggagga cgataacccg ctggccctga aatccgactt tgtgctgtcg ttctgtgagc
    12061 ttgtcatggg cggcaagaac gggctggaag ccattgaaaa gaccgtgata gaccgggctg
    12121 tgcgcgtgat ctaccggccc tatctggcag acccccgccc ggagaatatg ccgatcctgt
    12181 ccgacctcca caaagcgttg ttagatcagc atgtcccgga ggctgaccgg gtggcgcagg
    12241 cccttgacct gtatgtgtcc ggctccctga acgtatttaa ccaccgtacc aacgtggaca
    12301 tcggcaaccg gcttgtgtcc tttgacatta aggagctggg caagcagctc aaaaagctgg
    12361 ggatgctcat tgtccaagac cagatatggg ggcgcgtcac ggccaaccga agccaaggaa
    12421 aggccacatg gtattttgcc gacgagttcc atttgctttt gaaagaggaa cagacggcgg
    12481 cctattctgc ggagatttgg aagcggttta ggaaatgggg cggcattcca acaggtgcca
    12541 cccagaacgt caaagacctg ctttcctccc cggaaattga aaatatcctt gaaaacagcg
    12601 acttcattac gctgctcaat caggcgtccg gggatagaaa aatccttgcg gaacgcttga
    12661 atttgtccac cgaacagcag aagtacatcg acaattccga acccggcgag ggccttttga
    12721 tctttgaaaa cgtggtgctg ccctttacca accccatccc gcacaacacc cagctttaca
    12781 agattatgac gacccgctta aacgaggtgg cgggagtatg acccggcgac gccacaaagg
    12841 aggtgaacca ccatgaaaga gctgaaagcc aaggcgaaag tgacgcaggc catgacccgc
    12901 gacgggctga cgctggaaaa tcaggccacc ggcgaggtgg agaacatttc cagccgggag
    12961 gccgaacagg atttgtccgc gccggggccg ggcggcaccg ccgataagct gctggaacgg
    13021 gccgacgcca tccatgacgg ccataaggcc aagaaagccg cgaagcaggc cggggctgtg
    13081 gccgccgagg gggacgctgc cctgcaccgg ccatccgccc ggctccaact gtcggaggaa
    13141 gaacgggccg acccggattt acagccctat atccgaaaat cggacaaacg ggccgataag
    13201 ctggataagg cccgcgccgc catccccaag aagcgcgtcc cggtcaagga aaccgtcttt
    13261 gacgaggcaa gcggcaaagc aaagaccgtc ctgcactttg acaaacagga caaggggcct
    13321 ccctccctga aacccaatcc ggcaggccgt cctgtttccg aggtttttct attcggccac
    13381 gggaaaatcc atgaagtcga gcatgaaaac gtgggtgtgg agggcggcca taagggggag
    13441 gaacttgccg agcggcaggc cggaaaagcc atccgcagca ccatccggca ccacaggctg
    13501 aaaccctact gggacgcgga aaaggccgag cgcaggctat tgtctgccaa cgcggagtat
    13561 ttctaccaga aatctttgcg ggataacccg gagatcgcac aggcggccag caaccccatt
    13621 tcccggttct ggcagaaaca gcagatcaag cgccagtacg ccaaggccgc ccggacagcg
    13681 ggccagaccg caggcgcggc ggcacagggc gcggccaaga cagccggggc ctccacgaaa
    13741 gcggccaagg ccgcagccaa agaaagtgaa cgggcggcct cctttgtggc ccgccactgg
    13801 aaaggggcgc tggccgtcct tgtgttgggg cttttgatgc tgcttatcat cggcggctta
    13861 cagtcctgca ccgccatgtt cggcagcgcc ggaagcggca tcacggcgtc ctcttacttt
    13921 tccgaggata gcgatatgtt ggccgccgag gacgcctacg cggagctgga agccgagctg
    13981 caagactatc tggacaacta cgaggccacc catgactatg acgaatacca ttttgacctt
    14041 gacgagatca gccatgaccc ctatgtgctg atttccattc tgtcggcgct ccatgacggg
    14101 atatttacca tcggggaggt acagggtgat ttgtccatgc tctttgacaa gcaatatatc
    14161 ctgacagaaa acgtcgtggt agaaacccgt taccgcaccg aaaccgatac atggacggac
    14221 gcggagggca acacccatac agacacctac caagtgccgt atgactatta catctgctac
    14281 gtcacgctgg aaaacttcga cctgtcccat gtgccagtct atatcatgga cgaggaaacg
    14341 ctaagtttct attccctgta tatgtccacg ctaggaaacc ggcctgatct gttttccggc
    14401 agccagtacc ccaacgcctc caccctgacg ccgccgacct actacgacgt acccccggag
    14461 gctttggagg atgccacctt tgccgccatg ctcaaagagg cggaaaaata tatcggttat
    14521 ccctatgtgt ggggcggcag ctctcccagc accagctttg attgcagcgg cttcatttcg
    14581 tgggtaatca atcatagtgg atggaacgtg ggacggctgg gggcgcaggg cctatataac
    14641 atctgcaccc ctgtttccag ttcgcaggcc aagcccggcg accttgtatt tttcaaggga
    14701 acctacgaca cccccggcgt gtcccacgtc ggcctgtatg tgggcaacaa tgtgatgctg
    14761 cattgcggga acccgatttc ctacacgaac cttacttcaa actactggca atcccatttt
    14821 tacagttacg ggcgtttacc ttaacgccag aaaggagctt tcaattatgg caaccaaact
    14881 tgaccgcatc gaaaaggaca tccagaaaac caagtctaag atcgcggagt ttcagaaaca
    14941 gcttcgggag ctggaaacgc agaaaaccga acaggagaat ttgcagatca tccagcttgt
    15001 gcgcggcatg aacatgaagc cggaggaatt tgccgcgttc ttacgcagcg gcgcaatgca
    15061 ggccgcccct gccgcaaccc cttaccatga acaggaggac aatgcccatg aagaataaga
    15121 ccagacggat ttcccgtatt ctccccgcac ttctggccgt gatgctctgc gtcacggcct
    15181 ttcccgttac cgcccttgca ggcggcaatg acccggcacc ggccccgctg ccggaggcta
    15241 caaccgaacc cgctacgggc ggtttggagc ctgaaacaga cgaaaccggc accgccacgg
    15301 acgggacaga aaccgaggcc gaaaaagata ccggccctct gaccggcaag gacatcaccg
    15361 acctgttttc cgcgctgttc ggttccaagg tgagcattgc cgccacggac gacggcatcc
    15421 agatcaccac cggcaccgac aaaaaagaac ctgaacagac cggcaccgtc accaccaacg
    15481 gcggcaggct gaatgtccgc accggcgcgg ggcttgacaa gaccgcgttc acccagcttc
    15541 ccaatgggac gacggttgag gtagtcggca cagacggcga ctggattaaa attctcttgc
    15601 cggagcgcat cggctatgtc cattccgact atatgaccgt cagcgaaaag gaagtagcgg
    15661 ctaccggcga gggcggtttt tccctctcca ttgacccgga ggaaattgct tctctgctgg
    15721 aactgttcaa tggcggcggg gccagcgccg ccctgtcccc ggacgggaac ctgtcactga
    15781 ttgacgacat cggcagcgcc gcgaaaaatg gcaagcagtt tatcaccgtg gaaaccaaaa
    15841 acggcaatgt gttttatctg attattgacc gggacgacga gggaaaagaa actgtccatt
    15901 tcttaaatca ggtggatgaa gccgatctgc tgacgctgat ggaggacggg gaaaccgtca
    15961 cccccgctgc tgtctgctcc tgtaccacga aatgcaaggc cggggctgtg aacacgaatt
    16021 gcccggtatg taaaaccaac ctgaccgagt gcagcggccc ggagccgcag gaaccccagc
    16081 cggaggaacc cgaagccccg gaggaagaac ccaagggcgg cgcgggcggc ctgatcgtgt
    16141 tcctgctggt ggcgctggcg ggcggcggtg cggcccttta ctatttcaag ttcaaaaagc
    16201 ccaaggctga aaccaccggc agcgacgatt tgggcgagta tgattttggg gaggacgagg
    16261 atttggagga cgaggaaccc gaaaccgagt ttgacccgga agctgatttt gtgagtgaac
    16321 aggaggacga ggaatgacat ataaacttgt aatcgcggaa aaacccagcg tggcccgcag
    16381 cattgcgggc gtgatcgggg ccacggagaa acacgacggc tatttggagg ggaacggcta
    16441 tctggtgagc tggtgcatcg gacatctggt gtcctttgcc gacgccggac gctatgacga
    16501 gcgtttcaaa aaatggcgct atgaggattt gcccattctc ccggaaacat ggcagtacat
    16561 cgtccccgac gacaagaaac agcagtttga cgttctctgt tcgctgatgc agcgccccga
    16621 cgtgaccggc cttgtgtgcg ccaccgacgc cgggcgcgag ggggaattga ttttccgttt
    16681 cgtctaccag atggcgggat gccaaaagcc ctttgagcgc ctttggattt cctctatgga
    16741 ggacagtgct attaaggacg gctttgccca cctgaaaccg ggcggcgact atgatccgct
    16801 gtatcaatcg gccctgtgcc gggccaaggc cgactggctg atcggcatta acgccacccg
    16861 gcttttttcc gtcctgtacc acaagaacct gactgtgggc cgcgtccaga cgccgacctt
    16921 aaaaatgctg gtggacaggg acgccaagat taccgatttc aagaaagaga aatatcacat
    16981 cgtccatatc acggcgggcg gcgcggacgc ggtaagcagc cgcttttccg acgctgccga
    17041 ggcaaacacc gtcaaggcgg cttgtgcaaa agcgcaggcc gtttgcgttt ccgtgacgcg
    17101 ggagaaaaag acggaacagc cgcccaagct ctatgacctt accaccttgc agcgggaggc
    17161 taaccggctt ttcggctata ccgccaagca gacccttgac tatgcccagg cactgtatct
    17221 aaagaagctg ctgacctatc cgagaacgga tagtcaatat cttaccgacg atatgctgcc
    17281 cgccgtggaa agccttgtgt ccggcctgtg ggagctggtg ccgtttgcca agggcctgaa
    17341 cctttccccg cagtttgacc gcatcctgaa cagcaaaaag gtgtccgacc accacgccat
    17401 tatccccacc gctgaatttg tgaagcaggg ctttggcact ctggccgaga gtgaaaaaaa
    17461 gctgctgtcc ctgatctgct gcaagctctt atgtgccgtg gccgggccgc atatctatga
    17521 ggccgtcacc gccacattta cctgtgccgg gcaggagttt accgccaagg gcaagaccgt
    17581 cctttccccc ggctggaaag agattgaacg ccggttccgt tcttccttaa agaccgacgc
    17641 cgacgaggac gccgaggcgg tgcgggagct gccggagctt tgcgagggcc agacctttgc
    17701 agacgtggcc gcctctgtca ccgagcattt caccacgccg cccaagccct acaccgaaga
    17761 tacgcttttg tccgctatgg aacgggccgg ggccgaggat atgccggagg atgccgagcg
    17821 caagggatta ggcacaccgg ccacccgcgc cgccattctg gaaaagctgg tgcagatggg
    17881 ctttgtccag agaaaaggca agcagctcat tcccaccaag gacggcatca atctggctgt
    17941 ggtgctgccg gaggcgctta cttctcccca gcttactgcc gaatgggaaa accggctgac
    18001 cgagatcgcc aaggggcagg ccgatcctga cgaatttatg gacggcatcg agacgcaggc
    18061 gcgggagctg gtgcagacct attcctgtat cagtgaggaa aagcagaaat tgtttcaggc
    18121 cgagcgtgtt cctatcggga cgtgtccccg ctgtggtgag gctgtctatg agggcaagaa
    18181 aaactactac tgcggaaacc gggcctgcca gttcgtcatg tggaagaatg accgattttt
    18241 tgaggaacgc aagaaagcgt tcaccccgaa gatcgccgct gcgctgctca aaaccggcaa
    18301 agtcaaggta aagggcctct attccgtaaa gaccggcaaa acctacgacg ggacggtgct
    18361 tttggctgat accgggggca agtacgtcaa ctaccgcatt gaacacagga gctaaaggac
    18421 agtaacacag caagcatagg gagtgtggct acactcccgg taaaatcccc cggccgcgtc
    18481 cctgccgggc cggggatttt tgcttgttgg ggaagtcccc aaccccctat tttttgaaac
    18541 agaacagaga aaggagtgac cgcgtgaacg aagtattttc tatccaagtc cataaccggc
    18601 agtttttgga acagggcaaa caaggcgtct ggctggattt gcccaccaca accgaaacgc
    18661 tgcaagccgc cctgcgggat gttggcatta ctgccgacaa tccgcaggat ttttttatca
    18721 acgggtattc ctgcccggag gacagacacc ttgccattcc ctatgacatg gtgctggccg
    18781 ccgacgtgga cgagctgaat ttccttgccg cccggctggg ccagcttgac gccgccgaga
    18841 tcgcagagct gaacgccgcc ctgcaaaatc cccgtggcgg ctttgagaat atcggccaga
    18901 tcatcgacta caccgaaaat gtggattact atgtgcatct tcccgacgtt aggggggatg
    18961 gacagttggg cgactactac ctgaaccgtt ccggcatggt ggatatgccg gaggaatgga
    19021 aagccgggat tgatacggcg cagtttggac gccacattgc ccagcaggaa caaggcgttt
    19081 ttacccagta cggctatctg gtgagaagcg gcgacgaatg gcagcgcgtc catgagggcc
    19141 agccggtgcc ggaggaatac cgggtgatgc actaccccca gccggaagct gaccgggagg
    19201 aaccgcaggc ccagcagccg gaggcatccg aaagccccga caaagcgccg cccccggtta
    19261 tcccgatcat gctggacggc atgaacagca aagaacgcat gaaagtgatt actgaccgtc
    19321 tggaaaccgg cgtacaggag ctttttgaaa gtagccgcta tatggcttac ttatccgcta
    19381 tgtccaagat gcacgattac agtctgaaca atacgctgct gatcgccatg cagggcggtt
    19441 cgattgtggc gggctataac aggtggcagg atgaatttga ccgccatgtg aagcggggcg
    19501 agaaaggcat taagatactg gccccggccc cgttcaaggt taagcagcag atggagaaaa
    19561 tcgacccggc cacccagcag cccgtgatcg gcgcggacgg caagcccgtg accgaagaac
    19621 aggaagtgac ggttccggcc tttaaggtgg tgacagtctt tgacatcagc cagaccgagg
    19681 gcaaggattt tcccatgccc tatatcagtg aacttaccgg cgacgtgcag cagtatcagg
    19741 atttctttgc cgcgctggaa cggacatcac cctatcccat aagcattgag ccgatttcag
    19801 acggggccaa gggccgctgc ttctacgcaa aacagcgcat tgccatcaac gagggcatga
    19861 gcgaattgca gaccctaaag accgccatcc atgagattgc ccatgccaag ctccacgcca
    19921 ttgaccggga ggcccccgcc gaggaacagg cggcccggcc agaccgcagc acccgcgagg
    19981 tacaggccga aagcgtggcc tataccgtct gccagtatta cgggctggac acgtcggact
    20041 attcttttgg ctatgtggcc gattggagca gcggcaggga gttatccgag ctgaaagcct
    20101 cccttgaaac catccgggcc acggccagcg aattgataac caagatcgac ggccattttg
    20161 cccagcttca gcaggagcgc gaggcccggc aggaacagga ggccgtcacg gttgacgtta
    20221 ccccggaggc agcggccccg gcgcaggccg aggaagaaca ggccccggcc cttgatccgg
    20281 cagccgagcc gcttgtgact gtgctatgga gcgaaagccc ccagctaaag gacggccagc
    20341 aaatgccgtt gcatgaggcc gacgcgctgt ttgcccgtct ggatgcagca tatccggcag
    20401 aggcaggcta tgacaaaacc gcgttccgca ttgactgtat ctttcagggc gagccggaca
    20461 gctacgaggg gcggcaggat tttggagata gggacggttc cctgatcgaa catatccaga
    20521 gctaccacca attttacgcg caggacgaaa actggaaaaa gcatgtaatc gctacaaccg
    20581 gcatagaggg ctgggagcag gacaaggccc atcgggatat gctgttaggc gagtttgtcc
    20641 cctatatgaa gctgcattgc agccttgccc aaatggaaca gacggcgcag ggccgcttac
    20701 agtccgggga aaccctgacg ccggaggaaa cggcctattt gggggcgctt ctctcttatg
    20761 tgcaggagtg ccgccccatg ctcaatcagg gccagtatca gttgccggag ccgccgaaac
    20821 tggctgactt cgaccagagc ttgcaggact acaaagcaca ggtacaggcc gagatcgcgc
    20881 aggaggccgc cgcagccggt atgaccgtgg aggaatacgc ggcggctggc tatgaagcac
    20941 cccaacagga caccttttcc atctaccagc ttcgaggcgg ggaggaaacc cgcgactatc
    21001 ggtttgagcc atacgagcgt ttacaggccg cagggctggc cgtggacagt gccaactatg
    21061 accttgttta tacggcaccg ctggataaaa gcacgtcttt ggaggatatt taccgcacat
    21121 tcaacatcga ccacccggca gattttaggg gacacagcct ttccgtttcc gacgtggtgg
    21181 tgctgcacca gaatggacag gacaccgccc actatgtaga cagcttcggc tatatggaag
    21241 tgccggagtt tttgcgggaa aatcccctgc gtaccgccga actgtctacg gagcagaacg
    21301 aaaacatgat tgacggcatt atcaacaata cgccgcccga accgcagccg gaggaacccg
    21361 aacagctatc ttttgaggat gtggcagttg cttccggcca gacacaggag gaacccccgg
    21421 aggctgcgcc cccgcaaacg gctgtccgct attatcccat caatgaggga gccgcccgcc
    21481 gcgccaagga agccatgagc ttttctgact accggcccgg cagcgccacg gcggaatacc
    21541 ggcactatgt cgatcaggcc gtggaaattg cggagcgcca gaaaaagcgt gtggagccag
    21601 agtatcacga aaagatagac agcctccttg acacctacgc ccgcaagctg gccgagaaca
    21661 tgaataaggg ctatgagatt accgcccgcg tcccctctgt cctgattgcc gggccgtcca
    21721 atttcccggt acgggcgaaa gaaaagcaga acgccgccag tgaccgcaac atggaggaat
    21781 ttcagtacat tcagggcttg cttgacaaaa tccgcagcac cggcatgggt ggtatcagcg
    21841 ccgacgatcc gcaggccgtt tccaagctgg aaaagaaact ggaaaagctg gaagcgtcgc
    21901 aggagttgat gaaggccgtc aatgcctact accgcaagca tgggacgctg gacggatgcc
    21961 cccatctgac cgaaagaggc attgaaaacc tgaaagcgga tatggcgtcg ggctggcact
    22021 atgagaaaaa accgttccag tcctggcagc tctccaacaa taacgccgag atacgccggt
    22081 taaagggccg cattgaggaa ttgacccgcc aaaaggaggc ggcctatgtg ggatgggagt
    22141 ttgacggcgg cactgtggag atcaaccggg tggccaaccg cctgcaaatc ttctttgagg
    22201 ggaagccgga tgctgctgta cgcgacgagc tgaaaagcaa cggtttccgc tggtcgccca
    22261 aggccggggc atggcagcgg cagttaaacg acaccacaat ccgcgtcgct gaccgtatca
    22321 agtgcatcca gcccctttcc ggcgaaaagc ccagcgcctt acagcttgcg gcccgcaggg
    22381 agaaagaaaa gccctctatc cgggcgcagc tcaacgcggc caagaccgaa caggccaagc
    22441 agccaccggc gcgggaaaaa tcaaagggat tggaggtaga gtaaatgcag aagtttgaaa
    22501 atgtggacgt tctggccgcg ctggaacaga tcatgcggca gaacaccgcg ttttatcaaa
    22561 gtgactttgg cattgacaaa gacattatca ggcgggatgc cgccagcgat caggccgcag
    22621 acaagaccct gctctggatg tcccggccct ccgggacgta ctgtttccgg gaacgtgatg
    22681 ttttcctgaa agacacccga cagtacaaca catggaaatt ttacggggag cagacccgtg
    22741 acaagatact ggcctacgcg gtggagctga ccggcacaca gggcggcact atccggggca
    22801 atctctatga gctggactat ccgcagcatt tccggcacgt cattgaaacc gcccagccgg
    22861 tgagtgtgaa ccggctcttt tatgagcatg gcacccgtga catcccggag agccagcgtt
    22921 ttgacggaag ccctgaccgg gtattgggaa actttttgta ctatgaggcc cagccccatg
    22981 acccggctgt gctgcaggag gcgctacgga cggagcagca cagccggggg cgggccgtcc
    23041 ccggcgattt caaagtccac gtcaccgctt tgcatgacag ccggattgaa accgaggccc
    23101 ggcgtattgt ggacagcctg aaagatctgt ccgcgcctaa cagccccaat aaaacgcatt
    23161 tcatggtgga gatctccccg cactttgcgg cgattgcttc caccaaggat aatgaccggc
    23221 tctttgccat gctgccctat aaatccctgt gttttaccgg gatgaaaggc aggcatggcg
    23281 tgtatgccgt gattaaccgg gacgagcgcc gggatgtgag tattcgcagg ccccggccct
    23341 ctatccgcaa gcagctatcc gacagcaaac aggccgccag cccaaaaaag gcggcggcca
    23401 gaaccaaaaa gaatgaattg gaggtataag gaatgatgca ctttaccgtg gaggaagaaa
    23461 acctgatctg tatgtaccac aatgcagacc ggcgcagaac gataaacagg ctcaccgccg
    23521 tcatgccggt tatggatggg gatatgcggg cgcaggccca gcagaccctt tccaagctgg
    23581 aacgcatgac ggatgccgac tatgacggcc agaagttcca cttcacagac gaataacgaa
    23641 cagcagacgc cgggcgcggc ggccatgtgg tcgcttgcgt ccggcgtctt ttctttttgc
    23701 ctattcctac gcatttagaa cccggtattg tcggggatag gatgaagtat tcgcttgccc
    23761 ctgcaaacat gcgggagaag ccttgcagcg ggcggttttc aatcccttaa atgcgtaggt
    23821 tgcagctata attagacccc tgaaacagat atagccgcgc agcgggtgta tctgtttcag
    23881 gggtgtgcag gtgatagccg caaagcggcg taagagggtg cagccctctt gccgtctatc
    23941 tctgttccag cgggaagctc ggcaatgctt ccagcagttt cagggaaatg cgctgccgta
    24001 tatcttcgtc aatgactttc ttctttttgc cgtttgcctg tctgatctcc accgtggaaa
    24061 gctcgttgat gtagtcagca taatgggcca acactttttc cgtggcccac ctctcaccgg
    24121 caacggcggc tttgatcgtt tcatagtcca gcagcttttc ttgtccgtcc atcctgatac
    24181 ccccgtcaga ctgatttttt ctatgatacc atattcggaa gcatggaacc agagaaaacg
    24241 gcgcttttct gctggtaaat tctacgcatt tagaacagca cttcggcggg gataggataa
    24301 aacacccaca taccccgctg accatgccgg aaaagccttg aaatataagg gcttttacag
    24361 ctctaagtgc gtaggtttcc agtatctttt ccgctggcgc tcggcctctt tgcggcggcg
    24421 catccgtttg gagcaggccg cgcagtattt ggcccggtta gacttcggcg tgaacgtggc
    24481 cccgcactca cagcagcgtt tcacttcgtc ccggtctttg gtgatctcgg catacagggc
    24541 cgcatccagc gggaggacag cggccttaaa ccagcggcac agaagcgaat aggaaatgct
    24601 ctgtacgcaa atacattctt cgccgtcgtc cagcaacagg cagttgccgt cacaatagtt
    24661 gcagcactca tgcgtcagct tccgggcctt gcgatattga gggtaattca ttcgtggaat
    24721 actcatagct cttgcgtcca ttcctttccc ggcgtcatac gcaaaatgcc gtccacattc
    24781 tgcttgatga tgccgtattc ctgcatctgc tttttcgctt cgccgtactt ctcatacagc
    24841 cgttcctttt cctcggccaa tcgcttgtac tcggctttca gggcgtacag atcggggagc
    24901 tttttcacac cctgtccctt tagggccttt gccgcagctt catacaaaat caacgtgctt
    24961 tcatgctcac gccggaattt tgccttatcc gctgcattgc ggtattccat agccacgggc
    25021 cagagctgct tgtaggtaga aatattcttt atcagcaccg ccatatccgc aagccggtgt
    25081 tccatagctt tgagggtagc ggcggcctcg tcgctggcgg cggtgatctc ggccactttg
    25141 ctttccaaat ccgggtaggt gtctatccca tgttcagtca ggaaattcaa agtctttgcg
    25201 gcctgcttca aattgtggag cttcgcccac ctctcatagc ctgccgactg ctgggcctta
    25261 atgttgtttt ccagatcaat ccgcaaagaa atccccttgc gctctttggg cgctctggtg
    25321 ataacgcggc ctttgatacg ctctttgatg gcttcctcgg tgtaggcttc ccccaatgtc
    25381 ttgcagcggg taaagcgttc ctgtccggga gcgcggaaag aaataaactt tcccggcttg
    25441 atctcatagc cctgcgcctg catgagccgc aggaaatcat caaaatcttt tgcctgcggg
    25501 atagcggcgt caatggctgc tttgagcttc gctttccagc tcgtcccttt ccgctgggcg
    25561 tcccactcgg catagctctt gcccttactc tggccgggct tcaccacgga caatccgttt
    25621 tccttgcaca gcctgtcgct ggtgcgccgg atataggcat agctctgttt gttggaaatg
    25681 tacttgcgct gatttaccat atccacggca cagaaaataa tgtgattgtg gacgtggccc
    25741 ttatcaatat gggtagtcag cacataggga tatttccctt gcagcacttc ctcggcaagc
    25801 tgcttcccga tctcatgggc ctgttccggc gtggcttcgc cgggggcaaa ggattgtatc
    25861 aggtggtggc ccagattatt gcccttttgg tatgcctgcg ccagcagcat ttcaaattca
    25921 atgtccgctg tttcataaga gcaggcaaag gaggccacca gcagcttgcc gtcagttttg
    25981 tccgggttct cgatgtagtc aagggctttt ttcagcgttg acttgatagg atgtatcttt
    26041 gtaactgcca tatctgcgcc agcgccccct ttatatcctc gatgtcctgt gcgtacaccg
    26101 gccccatacc gttcagcctc tttgcaatct gattgacgtt gacgccgatc ttctgtatct
    26161 cggcggtctg cgcccggatg tcgctggtgt ccatgttgac gatatagccg tcaatgagca
    26221 tcttccgggc gtaggctgaa aagttcttcg tatgcagcag cgccattttc tgttcaatca
    26281 tagcccgttc ctccggcgtc acagggacgc gcagcacgat ttttcgcttt ctgtttgcca
    26341 tgcttcaccg tcctttccat tcaggggttt ggggatttcc ccaacaagca aaacggacgc
    26401 ggcccggcag ggacggggca ggccgttttt ccgggagtgg ctacactccc tatgcttgct
    26461 acgttactgt ttctccacta acaagacgtt tccaaagccc caaactaccc gcaaaatagc
    26521 cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc tgtgcagctc ccgctaaaag
    26581 taaaacagag gatgccagtt acacatcctc cgtttcattc ttcttcggct tttcaattag
    26641 tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat tcttcggggc agagtttcag
    26701 cttgtggctg accctttgcc gcagttcgct ttcctcccgc atgatctccg ggttaaagta
    26761 gcgttccaca ggaagcccgc aggctttaat aagctgtatg acaaccggca gactgggaat
    26821 ggccccgtcg ttttcgatgt tggcaagata gcgccagtca atatttattt tctctgccat
    26881 tgctttgcgc gaaacgcgct ttgcttgccg ggccgctttc acatccgcgc cgaaagtttc
    26941 aaagccggga caatcttcaa tattcgccat atcgcacctc ccacttacat tgtatatttc
    27001 atactctgcc cgtggaatgt tggtatatgc acctttctta aagtttatat ttcacatttt
    27061 gtttctttta ttcttcgagc aaaagaatta taatgtaccc tgtggaggtg tactatggac
    27121 tacatgacgt tgaaagaggc aggcgagaaa tggggtgtgc ccccacgatg gataaactac
    27181 tattgtgctg cgggacgcat ccccggcgct gtaaagatgg cgacgatctg gctcattccc
    27241 aaaactgcgg aaaagccgat tgacaggcgt acaaagaaag gaaaggcggt tactgaatag
    27301 tataatgaaa ccggcgcatt ttggatatgg ttttgaagag agagaaagca tggtattttg
    27361 tcacggaaaa actataagat gatagatggc aggctgctcc agaccgataa aaagtattct
    27421 catctgaaac agaaagaaaa gatttacaca tggatgtatg aagaagcgaa actttatcat
    27481 gacagaaatg gaaaatgccc gaaacaaaga cgaggaagct gctgctgtat cagccgtata
    27541 tggtagaatt gaaaaggccg gcatctggat tctttatggt gaagtctcca gacattggat
    27601 aactgctggt ctaatgcgcc tttgagtatg gggtgattga ttttaatacc ggggaggtac
    27661 ggctttttga ggaaaatatt ctgggattta catttggcga ttattacatt tcctgtaact
    27721 gcaaggacac ggatgatgaa gatatgagga atatagactt gctgattttc tactgtcttg
    27781 aacggtatta ggactgaatc tgctgcgcgg agtgcggcaa gagcgaccgg agggagtaca
    27841 cacaggccct gcctgtgtga aaagccatac cgcaaggtgc attcgcaaaa aagaggccgc
    27901 gcattttgct gaggcttggg gtgtccccaa caagcaaaac gagaacgccg caggcgggca
    27961 agtggtggat cagaatatcc gtaaaggata ttctgatcca ttgcttgcca gtattatcgg
    28021 gatgttttga caaccgctgt aatcgaaaca tcccaaacat taagaaggtg ttaattatta
    28081 ttatcttgat ttaattcgtt taaaccaata atatagatac atacaccatg aaaagaagcg
    28141 agtatcccaa tgacggatga ttaaaatccg aaaagaaatc gctttgaaac tacagggaaa
    28201 ctacagactg gctacaggga aagtacagat gcgcttgcca taataacaat cgtaccagcc
    28261 acaaatcgta gttttattgc aaaggaggca ttcgatcaaa tggaaaaaag caactatcat
    28321 tccaatgcgg atcatcacaa acggcatatg aaacaatccg tggaaaagcg ggcttttcta
    28381 tgtgcgttca ttatttcgtt cacagtctgc acgctgtttt tggggtggag actggcttcc
    28441 gtattggagg caacacagat accgcccatc cctgcaactc atacaggcag cagcactgac
    28501 gtagtggaga atttggagga aaacgctctt gccaccgcca aagaacaggg agatgaacag
    28561 gaatggagcc tgattttagt gaacaggcag aaccccatcc ccgcacagta cgatgtggag
    28621 cttgagcaac tatcaaatgg tgagcggata gatattcgga tttctcccta tcttcaagat
    28681 ttgtttgatg ccgcaagaac tgatggagtt tacccgattg tcgcatccgg ataccgaaca
    28741 acagaaaaac agcaagaaat tatggatgaa aaaattgccg aatataaggc gaaaggctac
    28801 acctctgcac aggctaaagc ggaagcagaa acttgggtgg ccgtgccggg aacgagcgag
    28861 catcagcttg gtcttgctgt ggatatcaat gcggacggaa ttcattcaac aggcaacgag
    28921 gtttatagat ggctggatga aaacagctat cgttttggtt ttattcgtcg ctacccacca
    28981 gacaagacag agataaccgg tgtgagcaac gagccgtggc attaccgata tgttggcatc
    29041 gaagctgcca cagagatgta caaccaaggg gtttgccttg aggaatattt aaaaccagaa
    29101 aaatgagaaa aggatgttat gttatggaca gaaaaagatt gacacagcgt ttcccgttcc
    29161 cgcttccaat gagacgggcg cagagaaaaa tgtgctttta tgcgggaatg agatttgacg
    29221 gctgtcgcta tgcgcagacg ataggggaaa aatcgctttc ccatttgctc tttgaaacgg
    29281 attgcgcatt atacaaccac aataccggat ttgacatgat ataccaggaa aacaaggtgt
    29341 tcaacttaaa gctggcagca aagaccttaa acggcttatt gataagaccg ggggaaacct
    29401 tttctttctg gtggctggta cgccatgcgg acaaagatac cccctataaa gacggcctta
    29461 cggtgaccaa tggtaaactc accaccatgt cgggcggcgg tatgtgccag atgagcaatt
    29521 tactattttg gatgttcctg catacgccat tgacaattat ccagcgcaga ggtcacgaag
    29581 taaaggagtt tccagagcca aacagtgacg agattaaagg ggtggatgca accatttcag
    29641 agggctggat tgatttaaaa gtgcgaaacg ataccgactg cacctaccaa atatgggtga
    29701 ccttagatga tgagaaaatc atcggtcagg tgtccgccga caaagagccc caagcattat
    29761 acaaaattac aaatggcagt atccagtatg tccgtgaaag tggcgggatt tatgaatatg
    29821 cccaggttaa acggatgcaa gttgccttag gtaccgggga aataatagat tgcaagctgc
    29881 tttatacaaa caaatgcaaa atctgttatc ccctcccgga aagtgtggat attcaggagg
    29941 agaaccaatg agaaaaagtg tgggcattac tgtttttgga tgcgaacagg atgaggcaaa
    30001 tgctttccgc gctttatcgc cggattttca tattatccct acgctgatta gcgatgcgat
    30061 atcggcagac aacgcaaaat tggccgctgg caatcaatgc gttagcgtag gccataagtc
    30121 cgaggtttcc gaggcgacaa ttcttgcgct gagaaaggtc ggggtaaaat acatttctac
    30181 ccgcagcatc ggctgcgatc acattgatac gactgccgcc gagagaatgg gaatctcggt
    30241 tggcacggtt gcgtattcgc cggacagcgt tgcggattat gctttgatgc tgatgctgat
    30301 ggccatacgg ggtgcaaaac ccaccatgca cgccgtggcg caacaagatt tcagattgga
    30361 tcgtatccgg gggaaagaac tgggggatat gactgtggga gttattggaa ccggccatat
    30421 cgggcaagcg gtcgtcaaaa ggctgcgggg atttggatgc catgtgctgg cctatgataa
    30481 cagccgaaaa atggatgcag attatgtcca gcttgatgag cttctaaaaa acagcgatat
    30541 tgttacgctc catgtgccgc tttgtgcgga tacccgccat ctgatcggtc agaagcaaat
    30601 tggagagatg aagcaaggcg catttttaat caacaccggg cgcggagcgc ttgtcgatac
    30661 cggggcgctg gtggaggcgt tggaaagcgg aaagctgggt ggtgcggcac tggatgtgtt
    30721 ggagggcgag gatcagtttg tttataccga ttgctcgcag aaagtgcttg accatccctt
    30781 tttgtcgcag cttttaagga tgccgaatgt gatcatcaca ccccatacgg cgtactacac
    30841 tgagcgtgtg ctgcaggata ctacagaaaa aacaatcagg aattgtctca attttgaaag
    30901 gagtttacag catgaataga ataaaagtcg caatcatctt cggcggttgc tcggaggaac
    30961 atgatgtgtc ggtaaaatcc gcaatagaaa ttgctgcgaa cattgatacg gaaaaattcg
    31021 atccgcacta catcggaatt acaaaaaacg gtgtatggaa gctatgcaag aagccatgta
    31081 cggaatggga agccgacagt ctccccgcca tactctcccc ggataggaaa acgcatgggc
    31141 tgcttgtcat gaaagaaagc gaatacgaaa cacggcgtat tgatgtggct ttcccggttt
    31201 tgcatggcaa atgcggggag gatggtgcga tacaggggct gtttgtattg tctggtatcc
    31261 cctatgtggg ctgtgatatt caaagctccg cagtttgcat ggacaaatca ctggcctaca
    31321 ttcttacaaa aaatgcgggc atcgccgttc ccgaatttca aatgattgat aaaggtgaca
    31381 agccggaggc gggtgcgctt acctaccctg tctttgtgaa gccggcacgg tcaggttcgt
    31441 cctttggcgt aaccaaagta aacggtacgg aagaacttaa cgctgcgata gaagcggcag
    31501 gacaatatga tggaaaaatc ttaattgagc aagcgatttc gggctgtgag gtcgggtgtg
    31561 cggtcatggg gaacgaggat gatttgattg tcggcgaagt ggatcaaatc cggctgagcc
    31621 acggtatctt ccgcatccat caggaaaacg agccggaaaa aggctcagaa aatgcgatga
    31681 ttacagttcc cgcagacatt ccggtcgagg aacgaaatcg ggtgcaggaa acggcaaaga
    31741 aagtatatcg ggtgcttgga tgcagagggc ttgcccgtgt tgatcttttt ttgcaggagg
    31801 atggcggcat cgttctaaat gaggtcaata ccctgcccgg ttttacatcg tacagccgct
    31861 acccacgtat ggtggccgcc gcaggaatca cgcttcctgc actgattgac agcctgatta
    31921 cattggcgtt aaagaggtga taagtatgga aaatggtttt ttgtttttag atgaaatgtt
    31981 gcatggcgtc cgttgggatg ccaagtacgc cacatgggat aacttcacag gaaaaccggt
    32041 ggatgggtat gaggtgaatc gcatcatcgg cacaaaggcc gtggcgtttg ctctgcgcga
    32101 ggcacaaatc catgcggctg cgcttggcta tggcttgctt ttatgggatg gatatcggcc
    32161 aagaactgcg gtggactgct tcctgcgttg ggcagcgcaa ccggaggaca acctcacaaa
    32221 agaaaaattt taccccaata tagagcgagc cgagttgatt acaaagggtt atgtggcctc
    32281 acaatccagc catagccgtg gaagcgcaat tgatcttacg ctctaccacc tggatacagg
    32341 ggaacttgtt tcaatgggaa gtaacttcga ttttatggac gaacggtcgc atcatacagc
    32401 aaaagggata ggggatgcag aggcacaaaa tcgaagatgc ttgcgtaaaa tcatggaaag
    32461 cagcggattt cagtcttatc gctttgaatg gtggcactat aagttgattg atgagccata
    32521 ccccgatacc tattttaatt ttgccgtttc ataatgaaag tattggtttt gtatcagatt
    32581 ttggatttga taaaatctat gctggtcatg gacaatcttt tactggaaag tggttaaagc
    32641 atcttttgta atacattgca aattatagta ttcaatctgc ccagcggcag aaaagaaaaa
    32701 aaccgctgtt gggcagacct ttcccacgcc cattttgtat tttgctggcg gcggttttga
    32761 gaaatcaagg ccgccgttct tttctggcag caaaaggtgt ggcggcccat aggaggatgc
    32821 cgctatgctg ttttgctatt gccatgctct aatccacttc caccactcaa aaaaagcctt
    32881 gcccctccac agggctattc cggttatgag tatgtactat accatgtaaa tcagcagcat
    32941 agcaaagcat tttctcgccc ggtaggtttg cgacctgccg ggcgattttt gtcgtctttt
    33001 gcggcccggc tgtccccaaa aaaatttttt gagaaatttt caaaaaggag gtatttagcg
    33061 ggtagctggc ggccttgtca aagtcttgtt agcggaaagg gagagaacca cctaagtccc
    33121 ccacagaaag ggggtgagaa gatggatgct atcccccgcg actatgaagc gcggtgcatg
    33181 ttcgatgctt tttgcaagac ggtactgcgt aacgaggcca aaagctactt ggctgaaatg
    33241 aagcgccgcc gtgaccgtga agtgtcacta agctctttgt cacaggcaga tttggacaag
    33301 ctctgtacag tagatcacta ccccagcgac agctttacgt tttcgtcgca tggctatgac
    33361 ctacatatca acaatgagct tgttgccgaa gcctttgccg cgctgcccag catggagcag
    33421 agtattttaa tcctgcactg tgttctggat atggcagatg gtgagatcgg cggccttgtg
    33481 ggaatgtccc ggagtgccgt tcagcggcac aggacaaata ccttatcgga gctgcggaaa
    33541 cagctaaagg cgttgatgcc gaagggaggt tagacgcgga tgaagaaccc gaactacaag
    33601 ggcattgagc ttttgcccct ttcggtgatc gaggccgccc gcgctgggga tgccggagcc
    33661 gtagagcgcg ttttgcagta ttacagcggt tacatgaaca agctgtgtac ccggacgctc
    33721 tatgacgaaa acggctatcc gcatgtgtgt ctggacgagg ccatgaagcg ccgtctggaa
    33781 atcaagctca tccacgctat cgtcacgaac tgacgaaccg gccccggcag catgaaacgg
    33841 cttacccttt ccccgtttcg gtgttttccc ggtggcctga tcgcaccttg ataaatatgc
    33901 ccgcaagata acggcggcgc atcatagggc atacggacac attctgtctg tgccgagccg
    33961 gacggctggt gcgccatgat acttttccct acgggaaaag cggagcgagg aacaccacgc
    34021 cgcaatgtga gcgtggcggc ttgcgggcga cgacgtacag gcaggactat gatactccct
    34081 tatagccaca gtccgagcgt taaaagcgtc gcaggcaatg ggtagggccg cgtgagaacc
    34141 acgcgggggt gaaagtcccg tggagctgtg ccagcagccg tccgtctatg cccctaaatc
    34201 acacaaacct ttgaatttgt gaaagggggc tgcattatga accatgatgt gcctatttgg
    34261 gaaaaataca cgctgacgat tgaggaagcg tctaaatatt tccgtatcgg agaaaacaaa
    34321 ttgcgtaagc tggccgagga aaatccggcc tctggctggg tcattatgaa tggcaaccgc
    34381 atccagatca aacgcaagca gtttgagaaa ataattgaca ctttggacac aatctaatag
    34441 tgaaaaaggg ccgcagatat gttataataa tttataagca tatatgcggc tctttccgtc
    34501 atggaaagga gcatgacgag atgtcagaaa aaagacgaga cagcaaaaat cgcgttttgc
    34561 ggtctggaga gagccagaga aaagacggac gatatgctta caaatatgtt gatacctttg
    34621 gaaaaccgca gtttgtttac tcatggaagt tagtgcctac ggataagacc ccggcaggta
    34681 aacgggagga tatttcactc cgggaaaagg agaaagaaat ccagaaagac cttgacgacg
    34741 ggatagacac catcggcaag aaaatgaccg tctgccagct ctacgccaag cagatcaggc
    34801 accggggcaa tgtgaagcac aacaccaaac tgggccggga acggctgatg cgtattctgg
    34861 aacaagatcg gctggggagc tgcccgattg acagtgtgaa gctgtccgac gcaaaggaat
    34921 gggcgcttcg gatgaaagaa aagggccttt cttataagac catcaataac gacaagcgtt
    34981 ccctgaaagc ggctttttac accgccatac aggacgattg catccgaaaa aacccgtttg
    35041 acttccagct tagtgacgtt ctggacgacg atacggaacc aaaggtgcct cttacaccag
    35101 cacaggaaga aagttttctg tcctttattc aaggtgataa ggtttaccaa aagcattatg
    35161 acgcaatcgt tattctgctg gggacagggc ttcgcatttc ggaactgtgc ggactgacgg
    35221 acaaagattt ggattttgaa aaccgggtaa tcatcgtgag ccaccagctt ttgagaaata
    35281 ccggcgtcgg ttactatatt gacgaaccca agacccaaag cggcgtcaga aaaatcccca
    35341 tgaatgagga agtgtatcag gcgttccagc gagtgataaa aaaccgcaaa ggtgcaaagc
    35401 cgttcattat tgacgggtat gccaatttcc tgtttctcaa acagaacggc tatcccatga
    35461 cggcggtaga ttatggcggg atgtttggca ggcttgtgaa gaagtacaac aagagccatg
    35521 aagaagcgtt gccgaaaacg acgacgcccc atgctatgcg tcatacattc tgcacccggt
    35581 tagccaatgc gggaatgaac cccaaagcct tgcagtacat tatgggacat tccaatatca
    35641 ccatgacgct gaactattat gcacacgcca cattcgattc cgcaagggca gaaatggaac
    35701 ggctggcagc ctgacacaag gccggtttta ctactccatt tactactttt gagggggaaa
    35761 tggtgagagg taataagaac atttgtaagt atctaacaat atcaaaaatg ccggaaaagc
    35821 ccggaaacac gggctatacc gacattcaag aacttctaaa aagataatct aaaatcccat
    35881 ataattttag aataaaattt taaaaaggca aaaaattcgc cattttagga aagcgtttta
    35941 aaaaagtgtt gcaatctttt aaaaaaatgc tatattaaaa atgaaccggt tcaaatactt
    36001 gtttaagtat aattaatagt gagaggaagt tgctctactg aagactttag ttatatggat
    36061 attgatgaca agcaacacta tgcaaagaaa gcggtcatca taatcgaaca acaagaaaga
    36121 gaaatcaaat gtaactggca ggagtgagaa tgtatggcat cgatccgtga cgtggctaag
    36181 atggctggtg tttcagtggg aacagtttcc cgttacttga atggtcagca actgaaggaa
    36241 aagaacatga aaaaaatagc agaagcgatt tcagcgttgg actataaaga aaacatcatt
    36301 gctaaagggt tgaagaataa tcgaagcttc tctattggac ttttgataaa cgggatttcc
    36361 agccgttttg ggtcggaagt tgtctcaggg atcgaaagag tagctgaaga aaatgggtat
    36421 agcttattat taagcgggtt tgccgatcag ccagaaaaaa ttgaacaaaa aattgaatac
    36481 ctgatgaagc atgtgatcga tgggctgatt gtcttcttat ccgaagaaga gtggtctgga
    36541 tttgaaatgc tcacaaaaat gagtattcca gttatcgcat tgaattgccc agaaggacca
    36601 gcaggtattg atacaatatt agtgaatgat cgagaaagtg tagcaaaagt gatcgctcat
    36661 catggtgaac aaggacataa aaaaatcgga tttattgctg ccactcaaac agactacgta
    36721 gcaagagaac gtttagcagg agcaaaagaa gcggtacaaa atgatccatc gatccaatta
    36781 gaggtcttta caggagacta ttctagaaaa agtggctatt atggggcaaa agaattatta
    36841 gaaaaaggag taacagcgat ttttgtcagc aattataata tgtcgatcgg tgctatcgaa
    36901 tgtttcaatc aagaaggtat cagaatcggg aaggatattg cgtttagtca ttatgattat
    36961 ttggataaaa acgatttgag catccttccg aaaatcacga ttacaccacc aacagataaa
    37021 atcggggaac aagccgcaga acagctgttg aaacgaatca aaaaagaaga ggaagaaaat
    37081 gaaaaagtaa ttgtgatgga aaatatcata catggaatca actaaaacga actaaaaaag
    37141 aaaaagaggt gctttgatga aaaaatatca atttccagca aatttttggt ggggttctgc
    37201 agcaagtggc ccacaaacag aaggaaggat ttcaggtgat ggaaaaggag agaacatttt
    37261 tgattattgg tacaaaaaag aaccgcaaaa gttctttgac caagtaggac ctgaaaaaac
    37321 ttcacaagtt tatacgaagt atcaagagga tgtacagttg atgaaacaga ctgggcataa
    37381 ttcatttcga acatctatcc aatggagccg cttgatacct gatgaatctg gaacaatcaa
    37441 cgaagaagcc gttcgtttct atcatcgtta ttttgatgag ttgattgaaa acggtatcga
    37501 accctttgtg aatctgtatc atttcgatat gccgatgtat ctgcaagaaa aaggcggatg
    37561 gttgaaccga gaaacggttg aagcctatga aaaatatgcc aaaagttgtt ttgaattgtt
    37621 cggttcgaaa gtaaaaaaat ggtttactca taatgaacca atcgttccag tcgaagcagg
    37681 ttatttatat cgatggcatt atccagaaga gtcagatatg aaaaaagcga tccaagttgg
    37741 ctatcatgaa gcgttggcct cagcgttagc catcaaatct taccatgaaa tggagcaagg
    37801 tggtgttatt ggtatcatct tgaatctgac accaagctat ccaagagacg aatcaaaccc
    37861 tgaagatgtt aaagcagcgc aaatagcaga tgcgttcttc aatcgttcat tccttgatcc
    37921 agctgtaaaa ggcgagtttc caagtgaatt gattgagata gtaaaagaac ttgatctcat
    37981 gcctgtgatc catccagagg atttgacggt catcaaagaa aatacagtag atcttttagg
    38041 tatcaattat taccagccac gccgggtaaa agcaaaagaa acaccaatcg atactaaaaa
    38101 cggtccaatg cctgaggatt atttcgacaa ttatgaaatg cctggtcgta aaatgaatcc
    38161 ttatcgcgga tgggagatct atgaacaagg aatctatgat attttgatca atgtacgaga
    38221 tcattatgga aatatcgact gctttatttc tgaaaacggt atgggtgtag aaaacgaagc
    38281 acgatttatc aaagaagatg gcatgatcca cgatgattat cggatcgaat ttgtccaatc
    38341 tcatctgaaa tatgtccatc aagcaattca agaaggtgcc aattgtttag gctatcatat
    38401 gtggacatgt atggataact ggtcatggac caatgcatat aagaatcgat atggctttat
    38461 ttcagtagac ttagcacaag atggaaaaag aacaatcaaa aaatcgggtc actggttcaa
    38521 agacgtatca gataaaaacg gatttgaggc ttaagaaaga atagttgaga gcaaatgcgt
    38581 gaaattctcc attcttacca ttttgtgaaa aagaagttca attatttacg atctttttca
    38641 acttttttgt ctttctattt cattttttgt atataataga atagtcaaaa ttaaatagga
    38701 gtggaggaaa gcatgaggaa aaaaagaccg tttcttctct tttggttatt cggttttttc
    38761 ttactgctta ttttatccgc agcattttca ggaaaaatga cagcagatct tgctcagaaa
    38821 gaaacagaaa aaatcgtagc aagcaaagaa tcagtagata atttcaaaaa agaattctca
    38881 acttattggt gggcggagta taaaataact gccaattacg aaaaaggcga attggacatc
    38941 tatttgccaa aaggaaaaga agaaaatcaa tttattaata tcgatgacgt acttagtgcg
    39001 atttctctca gacaatatca atttaaagac aagcatttga ccgttttgct tttgactcat
    39061 gatggcgaca aagtagcccg tttgaaaaat ggaaaagtga tcaccgctta ttctgaaaaa
    39121 gtagacaaaa aagtggtcgc cgatcgttta gcggaatgga aaaagacaaa tgaacaagtc
    39181 aagaaaacaa ctgtctcttc tcaaaaaaac gagtcgacag gaacaagtcc ttcagattct
    39241 ggcagtggac aaacgataac gggaagtgaa gaaaatcaag cctcaattat tacgaatgag
    39301 tcatcacaag gacaatatca atcctatgga caatgaaaaa cagaggttgt gaaatcagcg
    39361 ttttgacctg agtattagag
//
LOCUS       DACGLH010000236        20862 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.044, whole genome shotgun sequence.
ACCESSION   DACGLH010000236 DACGLH010000000
VERSION     DACGLH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.044"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            267..1304
                     /locus_tag="IXT21_003200"
     CDS             267..1304
                     /locus_tag="IXT21_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4870517.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IXT21_003201"
     CDS             complement(1393..1716)
                     /locus_tag="IXT21_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ4870518.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IXT21_003202"
     CDS             complement(1703..2410)
                     /locus_tag="IXT21_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ4870519.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IXT21_003203"
     CDS             2496..2837
                     /locus_tag="IXT21_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ4870520.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IXT21_003204"
     CDS             complement(3008..3856)
                     /locus_tag="IXT21_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870521.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IXT21_003205"
     CDS             4454..5317
                     /locus_tag="IXT21_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4870522.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IXT21_003206"
     CDS             5417..5899
                     /locus_tag="IXT21_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4870523.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IXT21_003207"
     CDS             complement(6162..6629)
                     /locus_tag="IXT21_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ4870524.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IXT21_003208"
     CDS             6752..7603
                     /locus_tag="IXT21_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4870525.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IXT21_003209"
     CDS             complement(7854..9812)
                     /locus_tag="IXT21_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4870526.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IXT21_003210"
     CDS             complement(9809..10681)
                     /locus_tag="IXT21_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4870527.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IXT21_003211"
     CDS             complement(11023..11895)
                     /locus_tag="IXT21_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4870528.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IXT21_003212"
     CDS             12023..12592
                     /locus_tag="IXT21_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ4870529.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IXT21_003213"
     CDS             12659..13396
                     /locus_tag="IXT21_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4870530.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IXT21_003214"
     CDS             13627..14895
                     /locus_tag="IXT21_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ4870531.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IXT21_003215"
     CDS             15382..15846
                     /locus_tag="IXT21_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4870532.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IXT21_003216"
     CDS             15830..16459
                     /locus_tag="IXT21_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ4870533.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IXT21_003217"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IXT21_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4870534.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IXT21_003218"
     CDS             18338..18784
                     /locus_tag="IXT21_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ4870535.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IXT21_003219"
     CDS             complement(18983..19498)
                     /locus_tag="IXT21_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4870536.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="IXT21_003220"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="IXT21_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6630 a         3703 c         4316 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DACGLH010000237        20164 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.050, whole genome shotgun sequence.
ACCESSION   DACGLH010000237 DACGLH010000000
VERSION     DACGLH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.050"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            9..1049
                     /locus_tag="IXT21_003221"
     CDS             9..1049
                     /locus_tag="IXT21_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4870537.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1147..1335
                     /locus_tag="IXT21_003222"
     CDS             1147..1335
                     /locus_tag="IXT21_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4870538.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1393..1746)
                     /locus_tag="IXT21_003223"
     CDS             complement(1393..1746)
                     /locus_tag="IXT21_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4870539.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2277..2699
                     /locus_tag="IXT21_003224"
     CDS             2277..2699
                     /locus_tag="IXT21_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4870540.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2696..2926
                     /locus_tag="IXT21_003225"
     CDS             2696..2926
                     /locus_tag="IXT21_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4870541.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3424..3624
                     /locus_tag="IXT21_003226"
     CDS             3424..3624
                     /locus_tag="IXT21_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ4870542.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3651..4844
                     /locus_tag="IXT21_003227"
     CDS             3651..4844
                     /locus_tag="IXT21_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4870543.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4912..6477)
                     /gene="guaA"
                     /locus_tag="IXT21_003228"
     CDS             complement(4912..6477)
                     /gene="guaA"
                     /locus_tag="IXT21_003228"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ4870544.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6536..7459)
                     /locus_tag="IXT21_003229"
     CDS             complement(6536..7459)
                     /locus_tag="IXT21_003229"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ4870545.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7655..8260
                     /locus_tag="IXT21_003230"
     CDS             7655..8260
                     /locus_tag="IXT21_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4870546.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8568..9584
                     /locus_tag="IXT21_003231"
     CDS             8568..9584
                     /locus_tag="IXT21_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4870547.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9725..11026
                     /locus_tag="IXT21_003232"
     CDS             9725..11026
                     /locus_tag="IXT21_003232"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ4870548.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11072..11734
                     /gene="deoC"
                     /locus_tag="IXT21_003233"
     CDS             11072..11734
                     /gene="deoC"
                     /locus_tag="IXT21_003233"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ4870549.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11755..12153
                     /locus_tag="IXT21_003234"
     CDS             11755..12153
                     /locus_tag="IXT21_003234"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ4870550.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12219..13301
                     /locus_tag="IXT21_003235"
     CDS             12219..13301
                     /locus_tag="IXT21_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4870551.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13513..15075
                     /locus_tag="IXT21_003236"
     CDS             13513..15075
                     /locus_tag="IXT21_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4870552.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15068..16201
                     /locus_tag="IXT21_003237"
     CDS             15068..16201
                     /locus_tag="IXT21_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4870553.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16201..17157
                     /locus_tag="IXT21_003238"
     CDS             16201..17157
                     /locus_tag="IXT21_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4870554.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17256..18422
                     /gene="deoB"
                     /locus_tag="IXT21_003239"
     CDS             17256..18422
                     /gene="deoB"
                     /locus_tag="IXT21_003239"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ4870555.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18446..19264
                     /locus_tag="IXT21_003240"
     CDS             18446..19264
                     /locus_tag="IXT21_003240"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4870556.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19281..19988
                     /gene="deoD"
                     /locus_tag="IXT21_003241"
     CDS             19281..19988
                     /gene="deoD"
                     /locus_tag="IXT21_003241"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4870557.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6453 a         3622 c         4333 g         5756 t
ORIGIN      
        1 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
       61 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
      121 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
      181 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
      241 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
      301 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
      361 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
      421 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
      481 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
      541 aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg
      601 gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta
      661 gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag
      721 ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc
      781 tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga
      841 aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca
      901 atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc
      961 aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag
     1021 taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata aatatggttt
     1081 cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg aaaggaggag
     1141 attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa aacaagacta
     1201 cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa atgtaagaac
     1261 gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc agacgccaag
     1321 acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt ttgtttatct
     1381 gaattttact gactagcctt caatatttct tgcttcattg ataccttttg ctagtgcttc
     1441 cattaaggat agttctttgt ctgtaaagct atccatgtat ttttctacct gtaaccttcg
     1501 agtgctttta atcaaattat ctgtaggtaa gaaaaattca tcaatagata catggagtaa
     1561 tgatacaaga tcatacagca cttgtgtact tgggtgttgt cctttgtttt ctatatttgt
     1621 taaataacgt gggtcaattt caatcattgt tcccacttgt tcacgagtga gaccccgttt
     1681 cattcgagcc tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat cttctttttt
     1741 acgcatacta aatcacctct atacatttta ctgttcctat ataaccataa acaggtatag
     1801 aaaaacatgt tatatagttt atgagttcct atagaacact gtggaattta ttaatgcgat
     1861 gaatccaatt tcaaatagaa aggtcgctga ttttatttac ctatacaact ttataaaatt
     1921 tagtcatact cctagaaagt agataaaatt ggcgacttaa tggagggaaa agtcgccatg
     1981 agaaagataa tctgtcatct agttctctta attaagttct tatcaaaaaa acttatccac
     2041 ctgtactgga tagatatgac catcaatgtg gttttgcaga ttacatagat agaaaaatca
     2101 aagcatatga acagagaaaa caatctgttt atgtgctttt ttggtcactt aataaatatc
     2161 ttttaggatt tctcttcatt tatgcaacaa atcccccgtt cgtgtcgggg tttagtatga
     2221 agggagataa agaagcacat gccactccct ttcacgacag aaagggggtg aaaagcatga
     2281 aaccatcttc ttttcaagaa accatagaaa atcagtttga ctatatctgc aaacaagtta
     2341 ttgaagatga acgaaaagat tacttcaaac atctagacag actatcagca aaagaagtct
     2401 cgttttctga tatagataac taccttgtca actctttttc tactgttgat acgtacgtca
     2461 ctgatttcca actatttaaa ttatttggat taagaatcaa tgttgaaagt gatttattaa
     2521 gtgaagccct gcgtcatctt tcagagaaaa aacggaacat tattttactt cattacttta
     2581 tggatatgag tgatgtggag attgcggagt tactaaattt gaatcgttcg actgtttatc
     2641 gtcatcgaat aagtggacta gccatgatta aagaattcat gaaggagtgt aaagaatgaa
     2701 aacagcttat ccaagggttc ccttccccct cattgtaaag gcgacagatg gcgacgtgga
     2761 agcaattaat cagattgtta aacattatcg agggtatacc tcaaaacgtt cattacgtcg
     2821 catgacagac gaatatggaa attctcatat ggttattgat gaaaccctac gtggcagaat
     2881 ggaaacgaaa ctcatctcta agattttatc gttcgaaatt aaatagtccc attttttcgt
     2941 ggaagcgtgt cgaatcgtat ccacgcttcc cgatttggga ggtttgttat tccatcaaag
     3001 catattgtgt gttcagtagg ttttgatagg ctaacaagcc aaacgttctt tgacaaccaa
     3061 ataaaggcaa tcgaatacgt ttcggtaaga aaagagctga tgaactaaca cgccatgacc
     3121 tacaaaatta gtgagcgatt catgttagta attcgaaagt aatctttagc aggattgctg
     3181 ccatgacttt cttatcgtga taatgatact cccgtatagc catagtccga gcgttagaag
     3241 cgtcacaggc aatgggtacg gctacatgag aaccatgcag aagtgaaatt cctgtgagct
     3301 ttgctagagc tgttcgattg ctaagatatt cttctcttgt cagtgtatta ttataaaact
     3361 atttgatata attataggtg aatattggag ttttcctagc ctataattgg gaaaggagta
     3421 ataatgataa cggtcacaaa aaaggattta gttgagctag gatatggaac ttctttttca
     3481 gcagacatca taaaaaaagc aaaagaattg atgattacaa aagggcatat ctactatcaa
     3541 tctcgaaagc tagatagagt ccctcgtgaa gctgttgaag aaattctagg aattcggttt
     3601 tcaaatgaaa acaggtctga gtagtttttg cacagtaagg agtaaaaact atggttaaag
     3661 acccaataaa aaaagcaaag aatggaacct attattttcg tgcaaatcta ggttatgatt
     3721 ctaaagggaa aaaaattcaa aaatatcgta gtggattcac tactaaaaaa gaagcaagag
     3781 aagaatattc gaaactgcta ttgatgaaac cagaagaatt aagtgagaat aaggataaaa
     3841 tgacgtttaa acattatatc tttgaaattt tccttccatg gtataagaca caggtaaagc
     3901 tcagaacata tgaaaatcgc ttaccaacta ttaagaagca tttctcttat tttgataaaa
     3961 tggctgtttc cgatattgag cctattgatg tgcaaaattg gcaactaaaa ttatcaaaga
     4021 agtgtaagtc ttcttatgta agagctgttc aaggactatt ttcagttgct atggatcgag
     4081 caattgtcct tgggataacc accacaaatc catctaagat tattggcaat gtgaaaaagc
     4141 aaaagtctaa aattgaattt tggacaaaag aagaatttga aaaagtaatc tctctaattt
     4201 acaaagaaga ttactatcaa cactttttat ttatctctct gtggttttta tttatgactg
     4261 gaatgcgaat tggtgaagct actgccattc aatgggaaga tattgatttt gactcaggag
     4321 tactaaccat caacaaaaca ctctattaca agaatcaatc gaattataga tttgttgaac
     4381 caaaaacaaa ggcgagcgtt cgtcatattg tattagataa atgcacatta acttatttat
     4441 ccgaatggaa ggaagtacaa caaaatctaa ttcagacaga ctttgtaatg agctataacg
     4501 gtattcctac ccaaaaacat acactatcat atgctattga acgctatgct aagctggctg
     4561 gtatccatcg aatcagaatc catgggttac ggcattctca tgcttccttg ttaatcagta
     4621 tgggagaaaa tccacttatc attaaagatc gtctaggaca cgaagatatt gagacgactt
     4681 taggaacgta tggacatctt tatcctaaca gtaatttcga ggtagctaac aaattaaatg
     4741 gagccatttc ctttaaagaa gcaaaacaaa attatgatac ttcaccaaag aatcaattta
     4801 ctgtagacta cttgcgaaac aaagaaatga aaaaagtgca ataaaagtgc agtaaaaaga
     4861 taagaagagc cagaaaccct tggtataaga ggatttctag ctctcctaca actattccca
     4921 ctccactgtt gctggtggtt tagaagtgat gtcatacacg atacggttga cgtgggctac
     4981 ttcgtttacg atacgtacag agattttttg caagacttcc catgggatac gggcaaagtc
     5041 agcggtcatt ccgtcgattg aagttacagc acggataccg actgtgtagt cgtaagtacg
     5101 tccgtcaccc ataacaccga cagagcggat ccctggaaga acagtgaaat attgccagat
     5161 atcacggtca agtccagcag ctgcgatttc ttcacgcaag atagcatcag attcgcgtac
     5221 gatttctagc ttgtcttcag tgatttcgcc aagaacacga ataccaagac caggaccagg
     5281 gaatggctga cgccaaacga ttgaatcagg catgcctaac tgagtcccta attcacggac
     5341 ttcgtctttg aataatgtgt ttaatggttc gatcaattca aattgcatat cttctggcag
     5401 tccacctaca ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag attcgataac
     5461 gtcagtatac aacgttcctt gtgctaggaa agagacgcct tcttcaccag caagttttgt
     5521 tgcttcgtca tcaaaaacat atacgaattc attcccaatg attttacgtt tttgttctgg
     5581 gtcagataca cctttcaact tgtctaagaa gcgatctctt gcatcgactt tgatgatgtt
     5641 caagccgaat tttccgccta gactttccat tacttgttcc gcttcgcctt tgcgtagcag
     5701 accgtggtca acaaagatag atgtcaattg gtcaccgatc gctttttgta aaagaacccc
     5761 aacgacacta gaatcaacgc cacctgaaag accaagcaag acttttttgt caccaacttg
     5821 ttcacggatt ttttctactt gcatatcgat aaagttatcc attgtccagt taccttcaca
     5881 gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg aatgacgtac
     5941 ttctgcatgg aattggatac cataaaaatt acgtgttgtg tcttggatag aagcaatcgg
     6001 acaatctttg cttgttccga ctgtttcaaa gccttcagga actttagtta ccaagtcacc
     6061 atggctcatc catactgttt gttgttttgg tgtttcttta aacaagacag cttgatcgct
     6121 tgttacttct aacaaggctt tcccatattc gcggttttta gcaggctcta cggaaccgcc
     6181 aagtttgtac gtgatcaatt gcataccata gcaaatccct aatacaggaa tacctaaatt
     6241 gaagatctca ggatcgatcc caaatgcatt ctcatcatat acactgtttg gtccgccaga
     6301 aagaatgatt ccctttggat tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa
     6361 taattcggaa aagactccaa gatcacgaat acgtcttgtg atcaattggt tatactgact
     6421 accgtagtct aatacgataa tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt
     6481 tattacccct atccctttaa attttatctc aaaatagtca aactatttta aaatgctaat
     6541 attttcgcat gaaaatcgaa tcgatgatat gattttcagt tttatggaga attatatcgg
     6601 ctcttcctcg tgtaggcaag atatattcct gtaaattttt caaattcaca tttttccata
     6661 catcttttgc cattttaaag gcttcttcac gatctccgat agcaaaacga taataataat
     6721 tctctggatt ttggaaagcc gtatccagta acgcaccaaa acgatccaaa taccattttt
     6781 cgatcagcac gggatcggca tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga
     6841 tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag atatcaggct
     6901 gctggatcaa ttcgtacttg tctggaatga tatcatacac atcatgtgag taaacaggta
     6961 cttttatctc ttcctgacca gattttacac ggttcaagaa atcgatcagc atctccatat
     7021 cataactttc aggaaatcct ttacggtcca tgattccacg ctcttttaat atacgatttg
     7081 gataaagaaa tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag gtacgagaca
     7141 aaatcgtctg cagcagtctg gctgttgtac tttttccaac ggctacactt ccggcgatcc
     7201 cgataataaa aggcggtact tctacgtatt tatgaagaaa taaaccttta ctcaagaaca
     7261 aagattcaaa ttctttcata taaagataaa tcagatgagc aagaggtaca tagatatctc
     7321 gaacatcctg catggaaatg cggtcattca aacttttgat attatctagt tcatcttgtg
     7381 tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc ttctcttgcc acacggtagt
     7441 agttcattgg atcattcata ttagcctcct gcgttctccc atcaatggaa ctattttaac
     7501 acaaaataaa cgcaaacccc accacttaag agagattgcc gatctttttg tataatcttt
     7561 ataatagaaa gaaacggagt cttattcttg acttgcgcac ggatacataa aaggatagac
     7621 tgttgtcatg aaagaaatga gaaggatgga agacatgaca aatcattact attctgaacg
     7681 cccagatacg gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca gaacctttca
     7741 atttgtaaca gattctggtg tattttcacg tgaaacagtg gattatggtt cacgtgtcct
     7801 gattgatgct ttcgattggg aagagttacc tgatgaagga aagatcttag atgtgggatg
     7861 cgggtatgga ccgattgggt tagctttggc atttgcgaca cagcggtttg ttgaaatggt
     7921 cgacattaat gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta accaaatcga
     7981 acatgttgat atccatcaat cgaatatcta tgaagcagtt catgaagaaa cctatgcagc
     8041 tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg
     8101 cgcatatcct cggttgaaga agggcggtac actgacaatt gtgatccaga agaaacaagg
     8161 agcgccgagt gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa
     8221 agataaagga tattatatca tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag
     8281 cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt atcagttctt tttttgttga
     8341 gaagttttta aaaacaaaga actatttttt cttcaaaaag aaatagtagg aacagagtcg
     8401 ttgtttttta cgataaaata aataagatac agaacttgta tagaaggctg ccaagtcggt
     8461 agataaagga ggtttttatc aaaaaacata ctttcctttt ttctctattc ttttcttggg
     8521 ggttttggta cactgatagg gaatttagat agaagggagc caataggatg aagctgacca
     8581 tcagagatat cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag attttgaata
     8641 acaaaggaag cagatttagt gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac
     8701 atcattataa gcccgattat ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg
     8761 gaatgatcgt ccctgatgtc actgatttct tcttctccaa actgatcgaa ggtgtagaga
     8821 gctatttgaa tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa
     8881 atgagatcaa gtatttacaa gagctttccc accgctcagt ggacggaatc ttgctggcca
     8941 caccaaatat tttacctgag aaatatgcat tagacagcgg tttttatcaa aaaatgccaa
     9001 tcattttaat cgatcgcgga ttgaatcgtc gggataacgg acggcttatc gtaaaggaat
     9061 atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa tggccatacg aagatcggca
     9121 tgctgaagga aaccactgga tactatcaat tagaagaacg tttcaacgga tatcgtcatg
     9181 cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt tgaacatgga gagttgactg
     9241 tccaaggtgg atatactgca tcaaaaaaat tactcaaaca taaagatatc acggctattt
     9301 tttgcgggaa tgatgccatg gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa
     9361 aaatcccaga agatatctca gtcataggat tcgatggatt aaagctgtca gaatacatga
     9421 ttccgcaatt gacgacagtg cagcagccaa gttttgatat tggattttat gctgcacgtt
     9481 tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa
     9541 aattaattat tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc
     9601 ttatgtaagc atttacaaag gctagttagt gcttttttta tttttagtaa accagtttac
     9661 taaagtggtt tacctttcct aaaaataatg tataataccg aatgaagaca aggaggatca
     9721 ggcaatgcga atggtggatc tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga
     9781 ggaaatcgag tacatcgtca cgaactatac gaatggaaag attccagatt atcaagtcag
     9841 tgcattattg atggcaattt tttatcaaga catgacaaat gaagaaatca cgaacttaac
     9901 attggcgatt gctaattctg gtgatgtcat cgatttgagt tctcttgaag gaataaaggt
     9961 agataagcat tcgacaggtg gcgttggtga cacgaccaca ttgattttag cccccctagt
    10021 agctagtgtc ggagtgacag tggctaaaat gtctggacgc ggtctaggtt acacaggtgg
    10081 tacactagac aagctagaag caattcccgg atttcagatc gagctttcgg atgaagaatt
    10141 tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa acttggcacc
    10201 ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg accgttgatt ctcttccgct
    10261 gattgccagt tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga
    10321 tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa
    10381 gaccatgacg agcattggga aactggctaa ccgagaaaca gtggcggtta tctcagatat
    10441 gtcagaacct cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac
    10501 attacaagga aatggtccag aagatctagt agaaatgtgc tacgccttag gaagccaaat
    10561 ggttgttctt gctggcaaag ctaagacaac cgatgaagca agaacgttgc ttcaagaagc
    10621 tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc aagggggaga
    10681 cccagcgatc gtggaacatc cagaacgaat tttgactgca cgctacacaa tggagcttcc
    10741 tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat
    10801 gatgctaggt gctgggcgta aaaccaaaga agaagatatt gatcacgctg ttggattgaa
    10861 actgcataaa aaaattggtg acactgtgac aaaaggtgag tcactattga cgatctatag
    10921 taacgacgaa gaaatcactt ctgtcattga attattatat aaaaatattg aaattggtga
    10981 atcggcaatg aagccaacgt tgattcatga catcatcaca gaataaagga aaaaagcgga
    11041 ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga tcgaccatac
    11101 tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa
    11161 ataccatttt tattctgtct gcatcaatcc agcatgggtt gcgctagcga aagaacaatt
    11221 acaaggtgag ccagtagccg tatgtacagt aatcgggttt cctctaggag caaacacacc
    11281 cgagacaaaa gcatttgaaa caacagatgc catcaataac ggtgcagatg aagtcgatat
    11341 ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga
    11401 agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt
    11461 attagataga gaagaaatca tccgagcttg cgaaatagca aaagctgcag gcgctgattt
    11521 tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag gtagaggatg tccgtttgat
    11581 gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga
    11641 agaagcaatg gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg caggagtagc
    11701 aatcgtatct ggttcaactg gtgaaggcta ttaatttttt caaaggagaa gctcatggca
    11761 gaactcaaac aagaatggat caacacagct attgaagcgt tagataaagc ctatgttcct
    11821 tattcccatt ttccggtagg cgcttgtctc gtaacggaat caggcaagat ttatcaaggc
    11881 atcaacattg agaatgcatc atttggatta acgaattgtg ccgagcgcac agcttttttc
    11941 aaagcagtat ctgaaggaga aaaaacattt acgcatctag tcgtagcggg gcacaccccc
    12001 gatcctatct ctccttgtgg tgcatgtcga caagtaatgg cagaattctg ttcccctgat
    12061 atgccggtta cgctagtagg agacaatggt gtgacgaagg caacgaccgt tcgtgcactg
    12121 ctgccttatg catttactga aaaagatcta taactcatgt tcattcagtc agctgactgt
    12181 tgaaaaataa aacaattatt ttaggaggct ttacagaaat gaaaaaagca aaattatttg
    12241 gtttaggtgc tgtcgcacta gcagcagggt tatttttagg tgcatgcggc aacaacggct
    12301 ctactgattc atccggtggc aaaagctcat cagacacaac aactgcagca ttgatcacag
    12361 acacaggtgg tgtagacgac cgttcattca accagtcagc ttgggaagga ctagaaaaat
    12421 gggggaaaga tcaaggactt tcacgaggaa atgatggctt ccaatatttc caatcttcaa
    12481 atgaatcaga ttacatccca aatattgacc aagcattgaa tgctggattc aaaacgattt
    12541 ttggtattgg ttataaacta aaaccagcga tccaagaaca agcaacaaac aatacaggta
    12601 cgaactttgt tattatcgat gatgtcatcg atggattaga taacgttgta tctgcaactt
    12661 tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc agcttatacg actgaaacaa
    12721 acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat
    12781 tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc
    12841 aatatgctgg tgacttctct gctccagata aaggacgttc aattgctcaa ggtatgtatg
    12901 cacaaaacgc agatatcatc ttccatgctt caggcggtac aggtaacggt gtattccaag
    12961 aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg
    13021 accaatcaga cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc acattgacat
    13081 ctacactgaa agcagttgga acagtagtag aagatttggc acaaaaatca gcggacggta
    13141 aattccctgg cggcgaacac actgtttatg gattgaaaga agacggtgtt ggtctaacag
    13201 aaggacaact ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt
    13261 caggtgacgt aaaagttcca gaaactcctg aagaaaacta aaaccatttc tatagccttg
    13321 ttagacacat gtttgacaag gctatggttt gattagaaca agaattgttc atttatccaa
    13381 gctgccttgt gacagtttgg ataagggaac aactccctga agtaatgttt gtttcaaaag
    13441 aagaacgctc actaagcgtg aatcttagtc aatcgttgcc ctagatagaa atcgttagga
    13501 tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa tgcagaatat taccaaagaa
    13561 tttggaacat tcaaggcaaa tgacaacatc aatttacaag taaaggcagg agaaattcat
    13621 gcgttgcttg gagaaaacgg tgctggcaaa tctacattga tgaacgtgct ttccggatta
    13681 ttagagccga catcagggaa aattttgatg cgtgggaaag aagtaaagat cacaagtccg
    13741 accaaagcca accaattagg gattgggatg gtccatcagc actttatgct tgttgatgcc
    13801 tttactgtaa cagaaaacat cgtgttggga agcgagccta gtcgcgcagg gatgcttgac
    13861 cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac agtatggatt gtcagtcaac
    13921 ccagatgctt acgttcgtga tatttcagtt ggaatggaac aacgggtaga aattttaaaa
    13981 acactttacc gaggagcaga tgtactgatt tttgatgagc cgacagctgt attgacccct
    14041 caagaaattg atgaattaat cgtgatcatg aaggaattag tcaaagaagg caagtcgatc
    14101 attttgatta cgcataagtt agatgaaatc aaagcagtag ctgaccgctg tacagttatc
    14161 cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg taacctcaca acaattagcc
    14221 gatatgatgg ttggaagaac ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa
    14281 gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg
    14341 aaaaacttga acttagaggt tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga
    14401 aacgggcagt cggagttgat ccaagctttg actggtttgc gaaaggcaga aagcggacat
    14461 atcaagctaa aaggggaaga catcactaat aaaaaacccc gaaaaatcac tgaacatggt
    14521 gtaggacacg tgccagaaga ccgtcataaa tacgggttag tcctagatat gacattatct
    14581 gaaaatatcg cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat
    14641 tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg
    14701 aatgaacttg ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc aattatcgct
    14761 cgtgaagtcg accgagatcc tgatctgttg attgttgcaa atccaactcg tggactggat
    14821 gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac aaagggacaa atacaaagca
    14881 gtgttactga tcagtttcga attagaagaa attttaaatg tttcggatcg tattgctgtt
    14941 atccatgaag gagaaattgt cggtatcgtt gatccgaaag aaacatctga aaatgaatta
    15001 ggtctattga tggcgggcta taccttagaa gaagcacgaa aagagttaga aaagggggct
    15061 ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt
    15121 tgttttagga ttgatcttag gcgcgatatt gatggctgct tttggttttc agcccatcca
    15181 aggttattct tctatgctag atgcagccct tggttcgcaa agagggatag gtgaaacgtt
    15241 aaggcaagca acgccgttga tttttacagc attaggtttt tctgtagcta actcagctgg
    15301 gtttttcaat attgggcttt ctggacaagc actttgcggg tggatcgtca gtgtctgggt
    15361 ggcacttgcg tttccagatc tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg
    15421 tgccttagct ggtgcgatgg cagcggctgt tccaggttta cttcgagcat tttttggaac
    15481 aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa gcacatttgc
    15541 tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg gactcttcca atatgatcag
    15601 tcaaaatgcc tcactgcggt taccatggtt aacacaagct tttggcggct ctcgcgtaaa
    15661 ctcaggatta tttttagcaa tcatcgcgct tattttcgtt tggctgctga tgaagaaaac
    15721 gactttagga tttgaaatcc gttctgttgg attgaatcct tttgcttctg agtatgcagg
    15781 aatgagtagt aaacgtacga tcgttttgtc gatggtcatt tctggcgctt tggcagggct
    15841 tggcggtgtg gttgaaggat taggaacgta tcaaaacttc tttgttcaaa cgacttcgct
    15901 atctattgga tttgacggaa tggctgtttc cttgcttggt tctggatcag caatcggtat
    15961 tcttttatca gcgatcttgt tcagtatttt gaagatcggt ggacttggca tgcagacagg
    16021 agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct ttgattatct tctttgtcgg
    16081 gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa aacaagaaga
    16141 gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac catgaagggg gcgaactata
    16201 atgtcgacta tcgaattaat tgccttgatc cttacttcta cactggttta ttcaacaccg
    16261 ctaattctaa cgtcactagg cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg
    16321 ctagagggaa tcatggtcat gggtgctttt agttccatcg ttttcaactt gacgatgagt
    16381 ccatcacttg gttctatgac accatggatc gctattttag tgggtggcgt cgtaggtatc
    16441 ctattttctc tgattcacgc agtagcaaca gtcagtttgc gtgctgacca tattatcagc
    16501 gggacagtaa ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa agcatggtat
    16561 ggtaaaggtc aaactgataa catcacgcag aatctggggt atttttcttt cccaggatta
    16621 gcagatattc cggtcattgg accaattttc tttaaaaata cattcttacc tgcttattgt
    16681 gctattttga ttgctgtcct agcttgggtg atcatcttca agacgaaatt cggcttgcgc
    16741 ctccgttcag ttggtgaaaa tcctcaagct gccgacacat taggtatcaa tgtatatgct
    16801 atgcgttatt caggcgtact gatttctggc ttgttaggcg gtatgggcgg tgctgttttt
    16861 gctcagtcga tttccggtaa cttttcagct ggtacgatcg ttggacaagg gttcatctcc
    16921 atggctgcta tgatctttgg taagtggaat cctttagggg caatgggtgc ctcattgttc
    16981 tttggttttg ctcaaagctt gagtatcatt ggcgcacaat taccagttat ttcttcgatt
    17041 ccgccagtat tgctgcaaat tgctccatat ttgctgacaa tcattgtgct ggttgttttc
    17101 ttagggaaag cttctggtcc aaaagcaaac gggaagaact atatcaaatc aaaataatct
    17161 tttagcgaat aaaaagggtg ggttacggga agtgtttttc gtctcgccct ttttgtttta
    17221 aaggcaataa aaataaaaag aaagaaggaa taatcatgtt taaacgcgta catctgatcg
    17281 tcatggattc agtaggaatt ggagaagcac cagatgccga taagttcggt gatgttggtt
    17341 ctgatacatt aggacacatt gcgaaagaag caggattgaa cgttccacat ttagagcagc
    17401 ttggtttagg aacgatccgt cctcttgaag gtgtggaatc agtgaaagat catcaaggct
    17461 atgcgacgaa attagaagaa gtttctgtag ggaaagatac gatgacaggc cactgggaaa
    17521 tcatgggatt gaatatcaaa actccatttc gtgtgtttcc taatggattc cctgatgatc
    17581 tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg
    17641 gaacagctgt tattgacgat tacggtgaac accaaatgaa aacaggcgat ttaattgtat
    17701 atacttctgc cgatcctgtc cttcagatcg cggcacatga agaaatcatt cctttagaag
    17761 aattatatcg gatttgccaa ttcgttcgtg atataacaaa agatgatccg tatatgattg
    17821 gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga cttcacacga acaagcaatc
    17881 gccatgatta tgcgttgaat ccattcggac acacagtcct tgattctttg aaagaagcag
    17941 gaaaggacgt gattgctgtt ggaaagatca acgatatttt caacggacaa ggaatcactg
    18001 aatctgtccg cacgaaaagc aatatggatg gcgttgacca gctgctgaaa gttatgaaaa
    18061 acgatttttc aggaatcagc tttaccaatt tagtagattt tgatgcatta tacggtcatc
    18121 gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt tgacttacga attccagaat
    18181 tgcttgaagc gatggaagaa gacgacttgc taatgattac agccgatcac ggaaacgatc
    18241 cgacgtttac tggtaccgat catacgcgag aatatgtacc gttattggtt tacagtaaaa
    18301 aaatgaaaga acaaggggca ttgcctcaag gatattattc agatatttca gcaacgattg
    18361 ctgagaactt tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat
    18421 aatcttttta aataggagaa agcaaatgac caagttaagc gagatgttac aagaaacgac
    18481 ccagtttatc cttgataaag gagtaaaaga aattgatttt ggtttgattc taggttcagg
    18541 attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg atttcttatg atgagatccc
    18601 gttctttcct gtttcgacag tagttgggca tgcaggaaag ctagtttatg ggtctctggc
    18661 aggcaagaaa gtgttagcca tgcaagggag attccactac tatgaaggtc attccatgca
    18721 aacagtgacc tatccagtac gtgtgatggc cggactaggg gcccattcag tcattgtgac
    18781 taatgcatgt ggtggtgtca acgaatcatt tgtaccagga gatttgatgc tgattaccga
    18841 tcatatcaat tttactggtg aaaatccttt gatcggtccg aatgaagatg caatgggccc
    18901 tagatttcca gatatgagtc aagcttatac gcctgactat caagcaacag caaaagcagc
    18961 agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat atgggctatt ctggaccaac
    19021 ttatgaaacg cctgcggaga tccgaatgtc tcggacaata ggagccgatg cagtagggat
    19081 gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc
    19141 ttgtattacc aacttagcag caggtatgca agcgaatctg agccatgaag aagttgtaga
    19201 aacaactcag agagtcaaac aatcttttaa agcattggtt aaagaaacat tgacattatt
    19261 gtaataggag gaacaataaa atgagtgtgc atattgaagc gaaagaaggc gagatcgccg
    19321 ataagatttt acttccggga gatcctttgc gagctaaata tattgcggag acttttttgg
    19381 aagatccagt ttgttacaat caggtacgcg gtatgttagg ctttaccggc aaatataaag
    19441 gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc atctatgccc
    19501 atgaattgat ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat
    19561 tgtcaaaaga cgtccatgtt cgtgatttag tactagcaca aggtgcagcg accagttctt
    19621 cgatgatcga aaaaaatttc caagcctttc attttcctcc aatcagtgat ttcaatctat
    19681 tactgaaagc atatgagata gctaaagaaa agggctatac ggttcatgta ggaaatgttc
    19741 tttcggaaga ttctttctac aaagatgatt tgacagagac gttccaatta gctgaacttg
    19801 gcgttttagg agtggaaatg gaagcagcgg ctctttatta cttaggcgcg aaataccatg
    19861 tgcagacatt aagtttgatg accgttagcg accatttgat caccggtgaa gaaacaacag
    19921 ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa acagcgatag
    19981 cagaataaga aggaaatgaa aaagctgccc tgcattaagt aataagaaca tccaagcaaa
    20041 aatagctcct catgtttttg acttggctgg acttattatt gcaaatgtaa gcttttgaac
    20101 accgtttacc aagaggttgt gaatctgccg tttaactctg agtcacaagg aacaatttca
    20161 aaaa
//
LOCUS       DACGLH010000238        15224 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.070, whole genome shotgun sequence.
ACCESSION   DACGLH010000238 DACGLH010000000
VERSION     DACGLH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.070"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            135..1637
                     /gene="lsa"
                     /locus_tag="IXT21_003242"
     CDS             135..1637
                     /gene="lsa"
                     /locus_tag="IXT21_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4870558.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1704..2231
                     /locus_tag="IXT21_003243"
     CDS             1704..2231
                     /locus_tag="IXT21_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4870559.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2442..3503
                     /locus_tag="IXT21_003244"
     CDS             2442..3503
                     /locus_tag="IXT21_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ4870560.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3506..4402
                     /gene="murQ"
                     /locus_tag="IXT21_003245"
     CDS             3506..4402
                     /gene="murQ"
                     /locus_tag="IXT21_003245"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ4870561.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4416..6398
                     /locus_tag="IXT21_003246"
     CDS             4416..6398
                     /locus_tag="IXT21_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4870562.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6463..6909)
                     /gene="nrdI"
                     /locus_tag="IXT21_003247"
     CDS             complement(6463..6909)
                     /gene="nrdI"
                     /locus_tag="IXT21_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ4870563.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7120..8544
                     /locus_tag="IXT21_003248"
     CDS             7120..8544
                     /locus_tag="IXT21_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4870564.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(8725..9498)
                     /locus_tag="IXT21_003249"
     CDS             complement(8725..9498)
                     /locus_tag="IXT21_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4870565.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(9495..10400)
                     /locus_tag="IXT21_003250"
     CDS             complement(9495..10400)
                     /locus_tag="IXT21_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4870566.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(10554..10874)
                     /locus_tag="IXT21_003251"
     CDS             complement(10554..10874)
                     /locus_tag="IXT21_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ4870567.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            11348..12034
                     /locus_tag="IXT21_003252"
     CDS             11348..12034
                     /locus_tag="IXT21_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ4870568.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12036..12980
                     /locus_tag="IXT21_003253"
     CDS             12036..12980
                     /locus_tag="IXT21_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ4870569.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            13179..13823
                     /gene="pcp"
                     /locus_tag="IXT21_003254"
     CDS             13179..13823
                     /gene="pcp"
                     /locus_tag="IXT21_003254"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ4870570.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            13902..15179
                     /locus_tag="IXT21_003255"
     CDS             13902..15179
                     /locus_tag="IXT21_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870571.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         4892 a         2687 c         3266 g         4379 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgtttttt cgatgtatgg gtctttttgt
       61 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
      121 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
      181 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
      241 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
      301 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
      361 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
      421 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
      481 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
      541 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
      601 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
      661 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
      721 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
      781 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
      841 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
      901 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
      961 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     1021 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     1081 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     1141 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     1201 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatcc cagccaaaac
     1261 atctgagcat tagttatgta cgccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     1321 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     1381 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     1441 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     1501 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     1561 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     1621 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     1681 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     1741 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     1801 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     1861 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     1921 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     1981 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     2041 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     2101 aagaaaatgt ctatctgtta gtttgtcata atgccttgat aagagtgatc aatgcttatt
     2161 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     2221 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     2281 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
     2341 aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct
     2401 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
     2461 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
     2521 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
     2581 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
     2641 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
     2701 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
     2761 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
     2821 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
     2881 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
     2941 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
     3001 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
     3061 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaatgttc
     3121 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
     3181 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
     3241 atgtcatacg aagcgcagat gcacgttttc gagagattcc gcctatccca gcaagaaata
     3301 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
     3361 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
     3421 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
     3481 ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga
     3541 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
     3601 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
     3661 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
     3721 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
     3781 tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc
     3841 tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt
     3901 gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg
     3961 tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca
     4021 agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt
     4081 gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc
     4141 tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc
     4201 aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga
     4261 ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt
     4321 catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg aagcaaacgg
     4381 atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg
     4441 aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg
     4501 tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg
     4561 aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca
     4621 tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat
     4681 taggagaaaa actttcttct gtaagtgcag ctacagcaaa ttcatcagca tctggaaaag
     4741 accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat
     4801 ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat
     4861 ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg
     4921 gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg
     4981 ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa
     5041 caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag
     5101 caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag
     5161 gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac
     5221 ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc ggtctgattg
     5281 aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca
     5341 actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc
     5401 cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg
     5461 aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg
     5521 cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag
     5581 gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac
     5641 ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg
     5701 gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat
     5761 tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg
     5821 gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag
     5881 cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg
     5941 aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa
     6001 gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg
     6061 gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg aaacacgcta
     6121 ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg
     6181 acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata
     6241 cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga
     6301 tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag
     6361 caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg
     6421 aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc
     6481 taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa
     6541 gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa
     6601 gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag
     6661 atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc
     6721 gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt
     6781 ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgcat gcattttttc
     6841 agcatattct gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg atatatataa
     6901 aatgttcatg gatcatcctc caattctttc cttttagtat agcatctttt ttaagttttt
     6961 tggtggtgta atttggaaag ggactaggcg ctttgcttgt ataatgagaa aaaagccggt
     7021 agaattatat ttctattaaa gtttttatta attaagtaaa aataaaaaag gtttgttata
     7081 ctttttgata aaagcggttt tattttggag gatgcttaca tggatacaga gcaacagaaa
     7141 aaaggaattg gtttaattcc acttgctgcc ttagtaattg gttcagcgat tggaggtggc
     7201 gtatttggta ttatgacgga catctccgga tcggctggcg gcccagctat tttcagttgg
     7261 gtactcgtag gaacagcaat gttgatgctc tccttgtcga tcaataatat caataaaaaa
     7321 cgtccagatt taaaaggcgg gatctttagt tacgcagaag aaggttttgg ccgtttcgct
     7381 ggttttatca gcggttgggg ttactggctc acatgctggt taggcaacgt agcttttgca
     7441 acattgttga tgagcgcatt aggatatttc ttcccagtat ttgaaggtgg gcaaaatctg
     7501 gcatcgatcg tgatgagtac agtcttttta tggggatatg catggctagt gaatcgggga
     7561 gtcgaaaatg ctagtttcgt caatgctatc gtcacattct gtaaattagt gccattattt
     7621 ttatttattg tagtagctat cagcacgttt tcgatccaac aattcattga taacttttct
     7681 agcgacatta tattgaatgc gaatggacag ccgatcccgt tttccagtca agtattgagt
     7741 tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag gagcctctgt cgtaggttca
     7801 cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc tagggatatt cggcttgatt
     7861 attatttacg tcttggtttc catgcttcca tatgggacaa tgacgatgaa tcagctgcaa
     7921 acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa tgatggttgg taaatgggga
     7981 gcagtcgtga tcaacggcgg tttgatcatt tctttagttg gtgtttggct ctcatggacg
     8041 atcttaccga ttgaaacaat gggaaacatg gctgaagacg gacttttgcc taaagcatgg
     8101 ggaaaactca acaaaaatgg ggcaccaact tatgctatcg tattgacgac gttatgtacg
     8161 aatgtctttt tactttcatt attattcact gatagtgctt ataactttgc ttacacactt
     8221 ggtacagcag ctattttctt cacgtggctt ttcttaggat tgtaccaaat gaaactttct
     8281 tatcagcgga aagaatgggt ccaattttta gtagggcttt tagcaagtgc ctttcaagta
     8341 tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg ttcttgttct tttcttgcca
     8401 ggcatttatt tttacctcaa agcacgtaag gagcaaaacg agcctgtaca acatatccac
     8461 ttagataaga ttttacttat aggtgtgacg atggtcggag tgattgcaat ggttttattt
     8521 gccactggga tgcttcacgt atagaagtat aaaagaagta taaagagagg ttgtgagcct
     8581 gtcgtttatt caaaaacata ctaggaaagg agctgtgaca caaatgttgt cacagctcct
     8641 ttttcagaat aaacggtgtt taagagacag ccaatcggca ataaggctga acgtctcttt
     8701 cacagcctct tttttgatga gtcatcatac attttttcgc ttaaatacga gatatcctaa
     8761 tgctaagaac aaaacgatgt aagctaaatt accgaatagc atttgattag tagaaagatg
     8821 agtcaattgc ttcatgatct cttcgtttcc aatttggttt tgcagattca acatactgat
     8881 cggattccat ttgatccact cccatttttg gatgagtgca ataaggatac cagatatgat
     8941 tgacgaagca aaataaaaga cgattcctgc tgagatggat gcaccggaac tattgataaa
     9001 gcaagcaatc atcaacacta aacttaaaat cagccataaa ccgatataac tacccaaagt
     9061 ataagtgaac aagctttgga tcagtgtttg accagtagaa accttctccg tgaaggaaat
     9121 cgaagggaaa agaatcagtt tcatcaacac agtgacaata atagctaaaa ggtataaata
     9181 aacagaataa ataactaatg tgatccattt gcttactaat atttctcctc gagaatactt
     9241 tcgataaagg agatttttca atgtaccata ttgtgcttcc atcgaaatga tcgtgcttgc
     9301 agctgcaatc atgataaaga caatccaaga tgtggcacta aaaagctgag cagtcatttc
     9361 aactggatca atgatcgtcg tgtatttttt ggctagaagt gctgttccta tgagcaaaac
     9421 aactaataga acagataaaa cagcagttga ctttttcttg atcaatttaa ataattcttg
     9481 ttttacaagc aatgtcattt ttcttcttcc ccctttactg aatccagaat ttccaataga
     9541 gaagattcca aatcattcgt cagatgctgg acgtctgtca catggatatt ttgtgcagac
     9601 aatagctgga tcacggttcc taataattct tcgttttctt ttaaaagctt gattttatca
     9661 ggatcttcca aagtatatcc agcttctgtc aagatcgttc gagcagctag atcatttgtt
     9721 gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt cagccattgt acattgtttg
     9781 atgatctttc cttgattgat caggatcagc tcatcgacca ttttttcaag ctcgctgagt
     9841 aagtgactgg agatcaagaa agaggtgccc tgagtagcca agtcggtaat gatttttcgc
     9901 acatctcttg ttgcttgtgg atccaaaccg ttcatcggct catctaaaat aacgatagac
     9961 ggatggttca gtaaggcaag agcaattccc aatttttgtt tcattcccaa cgagtaggat
    10021 ttcgcttttt tgtgaatgta cttttccatc ttgagctgat tcacgatagt gtcgatagca
    10081 gatacatcgt tggtttcaga gaataacttc agatgatcat agcctgttaa gaatggatag
    10141 atacccgggt attcgatcaa tgctcctact tgttccaacc ctttatgact agagggagaa
    10201 atctcatttt caccaataca gattgttcca tctgagtaat ggatcagtcc taaaatagct
    10261 ttcatgatcg ttgtttttcc agcgccatta ggacctacta atccaacgat tttgccaggg
    10321 gataagctaa aactgacatc gctgagggct tgtttgtgac caaagttctt ggataattct
    10381 tggaccgaaa gacttttcaa aaatattcac tccttttcta tagatagtac aagcatacac
    10441 ctgatgagtg gagaagtcgc taggtcattg gacttatttc ttattttttc ataaaaaaat
    10501 tgaatattaa ataaaaaggc ttattttttc tgatcagtac ttattccaag tgtctagtgg
    10561 ccgctagtaa tcttgatgcc aatgatactg atgagtagca tccccacaaa gaaccaagtg
    10621 gaaagattca gcttatcatg aaacaagacc acaccaatta tgaccgaacc aactgcgcca
    10681 atccctgtcc agataggata agagagactc atcggcaaac ttttgattgc ctttgacaaa
    10741 aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag aataattgat tttagaaaaa
    10801 ccatcactcc atttcatcat cgttgaccag aatacttcca aaattcctgc tatgattaat
    10861 tccatccatg ccatacttac attcctcctt aactataaaa aaatacacaa aaagaggaaa
    10921 atcatccctt aaaagaccta atggaatgat catcctcttt atcctacccg gtggcagatc
    10981 gtatatcttt ttctgtagta tagcacacct atccagatat cggcaatctc atacttttta
    11041 gtaaagtgta ccaagcactg acggatatat cgttagcgga aaagaagatg aatgctttgc
    11101 aatttcatag aaagaagttg ttatttatta atcatttaaa aaaaacagta actagacctt
    11161 atttctatat attatcgggc aataaattaa aaaaagcttt accttacaat aagtaagata
    11221 atttttgtga taaaaaaata acgaaaaata tttaaaaata gtgtctattt cgtgagaaaa
    11281 caactatatt ttttatgtgt tttgctttat aattaaaaaa atattgtcat tttatggagg
    11341 aaaatatatg gaaaattatc ttagcctttt aggcgtcttg attgttattc tcgggtttgc
    11401 atttaaatta gactcgatat tgatcgtaat ggttgcttta gttgttacag ctttgactgg
    11461 cggtcttggc attcaaggaa tgttagaact tttaggtaca agttttgtaa acaatcgcgg
    11521 gatggcgatt tttatcatca ttatgttggc aacaggaacg cttgaaagaa atggtctgaa
    11581 agaatcagcg gcttccttga tcaagcgatt caagaaagta tctgctggta tgattgtaga
    11641 tatttatggg atctttcgaa tgatctttgc ggcttttaac gtgagttttg gtggagttgc
    11701 tggatttgtt cgtccaatca ttttaccgat ggcattgggg accatcgaat caaaagggtt
    11761 agaagtccat cctaagcatg aagaagaact aaaaggtatg gcttcagcta tggaaaatgt
    11821 ctgctggttc tttggccaag tactattcat cggaggagca ggggcgttgc tagtccaatc
    11881 aacactgaaa gatctaggat atgacgtaac attgggacag cttgctttag tggaagttcc
    11941 agtggctatc acggctttag ttgtggcaag tgtctacttc tatcttaaag aaaagaaatt
    12001 agcgaagaaa tattatccag gaaaggggca ataagatgga cttttttaca aatcctgata
    12061 ttttattagg cgataaatta ctggagatcc tttatatcgt gatgggactc gtattgattt
    12121 acactggagt aagaaattta acagataaaa ccaatcctca tcgttatggg tcagcctttt
    12181 tctggacggt cttaggggta gttattgcag gaggaagatg gcttccggca atcgtgaacg
    12241 gtggcttgat ctttgcgatg accataccag caattgctaa gaaagtcagc aaaggagaaa
    12301 gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga caaactagga atgaaaattt
    12361 ttattccagc tttgagtatc ggtgtctttg cgatcttgtt tgctctattt accaatttag
    12421 gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat gctaatcttg atgttctttt
    12481 ctcgagataa tcgaccaagt acatttttag atgatgcagc agatatgctg ggcacagtag
    12541 ggccgttaag catgttgcca atgcttttag cttctctagg tgcggtattt acaagcgctg
    12601 gagttgggac agtgatttcc aatgcagtag gtacgatcgt tccagaagga aatgtgaaca
    12661 tcggcatcat catttacgct cttggcatgg tgatatttac ggtaatcatg gggaatgcat
    12721 ttgcggccat caccgtgatg acggttggga tcggtgcacc attcgtattt agtcatggag
    12781 cgaatccagc gttaatagga atggttgcat tgacttgcgg tttttgtggg accctgctga
    12841 caccgatggc tgctaacttc aatatcgtgc ctgtagcgat gctggagatg aaagacaaat
    12901 acggagtgat caaaaaccag ttgtttattg cgttatttat gctagttttc caaattgctt
    12961 atatgattct atttaaataa tagaagagag gggttgtgac agaataattg tcgcaacctc
    13021 ttctaacatt gtagtggaac actatttttt tataagaata tattaagttt gatctgttat
    13081 gttatttaaa ctattgtttc ctttatttac gctgttcaag gaaaatggta tgataaaaga
    13141 taaccatatg ttaaatcaat cggacggagg aacaacagat gaaagtacta gtaacaggtt
    13201 ttgatccatt tggtggggac aaagtgaatc cagcctatga agcagtaaaa aaaatgccag
    13261 atgaaatcag cggtgctgaa atcatcaaag tggaagtacc gaccgtattc gaaaaaagca
    13321 gtcaagtagt caaagaagcc atccaacagc accaaccaga tgtcgtgatc tgtgtcggac
    13381 aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc aatcaatcta gcagaagcaa
    13441 gaatccctga taatgaagga aatcagccat tcgatacggc tttagaagaa aacggtccag
    13501 ccgcttactt tacctcattg ccaatcaaag caatgacaaa aaatgtacaa gatcatggtc
    13561 taccagcgta catctcttat acagcaggaa catttgtctg caatgatatc atgtatcgtt
    13621 tgcttcattt gatcgaaaca caattcccgg caattcgagg tgggttcatc cacgtgccat
    13681 tctcacccga tcaagtgatc gatcgtccag tgggtacgcc ttctatgtct ttagaagaca
    13741 ttgcttcttc tttgacttat gcagttgaag cagcagtaga aaataaagaa gatatttccg
    13801 gaaatgccgg aacaactcac taatttagca agaaacactg gctggttgct aacgttatgt
    13861 tagtagccag ctttttgtaa ttagaaagga aagaaacagc cgtggaagaa tggcaatccg
    13921 tctttgaaga atggtttcca aaagaaatca gcaaaagcta tcccatcaaa atttctaaac
    13981 aatacacaag cagtcaacgt tgggagatct atgcaaaatt aacgaaaaaa cagcgggaac
    14041 tagtcgataa gcaccgccga tatctaatca gctctcgttt tatggaagaa cattatttag
    14101 ctgcaacaga ttgggtgttt tctgacttca agatcaatcc gttttttcga acaaaacgaa
    14161 gccagcaaaa attgtattgt gagtgtggta gagaattaaa agtgcaatac atcgttaaat
    14221 cgccaaaaac cggaaagatc ctaaaactag gcatcaacca ctttgccgat cacctgcatg
    14281 tctctccgac agtggcagct tcgatccatc agggaatgac aaaagttgat ctggccttag
    14341 acgaattatt atggcttaag cagaagaata tcgattttcc tgaaggacta tggcaaaaat
    14401 actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc atatttaccg gatataaaat
    14461 tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc gatttacata gcggattatc
    14521 aagcattgga aaatgaaatc aagaaaataa gtgaacacat caatgggcag tcaaaaaagc
    14581 gacagataaa aaaagaacta tttgacgact ttgcagaaga attagtaaag gatgtagaag
    14641 aattcttgat caactatcgc gcatttttac gtaaagattg gcaatccatc gtatatgaag
    14701 aagtgccagt acatcctaat gcttattttg aaacatttat ttccgtgttg agaaagacaa
    14761 aacgtcaacg aacaccagaa gtcactgctc agatggaata ttttgcgaaa aaccaacgat
    14821 tcatccaacc aaaaatctat ctatttattt ggaagcaata ttgtcgctat ggttttacag
    14881 aagggttctt cgacagcatt cctcggatag tcagaaatgg ttttttaaaa gtattgcgta
    14941 aagaacgaga agctatacaa tctgctgata agaaagaccg tacagtctca aaagaaaaat
    15001 ggcaactagt agtgaaagat atccaatctg gaaatgtgca agagacaatc gacaaatgga
    15061 aaggaaaaca ttatcgtttt actgaagctc agaaacaagc gttggaatat tatcagaagt
    15121 tagaagaatc actgcgtttc aatgatgagg caagaaagta tctgaaggaa ttactataaa
    15181 aaatagaggc tgggacagaa gcgtttaact ccaagaaata agaa
//
LOCUS       DACGLH010000239         8061 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.114, whole genome shotgun sequence.
ACCESSION   DACGLH010000239 DACGLH010000000
VERSION     DACGLH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.114"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            128..1564
                     /locus_tag="IXT21_003256"
     CDS             128..1564
                     /locus_tag="IXT21_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4870572.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IXT21_003257"
     CDS             1753..4200
                     /locus_tag="IXT21_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ4870573.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IXT21_003258"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IXT21_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ4870574.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IXT21_003259"
     CDS             complement(5690..6793)
                     /locus_tag="IXT21_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4870575.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IXT21_003260"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IXT21_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ4870576.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DACGLH010000240         5775 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.134, whole genome shotgun sequence.
ACCESSION   DACGLH010000240 DACGLH010000000
VERSION     DACGLH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.134"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(85..3009)
                     /locus_tag="IXT21_003261"
     CDS             complement(85..3009)
                     /locus_tag="IXT21_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ4870577.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3155..3730
                     /locus_tag="IXT21_003262"
     CDS             3155..3730
                     /locus_tag="IXT21_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4870578.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3944..4639
                     /gene="vanR"
                     /locus_tag="IXT21_003263"
     CDS             3944..4639
                     /gene="vanR"
                     /locus_tag="IXT21_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ4870579.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4617..5768
                     /locus_tag="IXT21_003264"
     CDS             4617..5768
                     /locus_tag="IXT21_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4870580.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPGFCCKVLNKE"
BASE COUNT         1782 a         1090 c         1089 g         1814 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgtc taaaatactt tgttttttta
       61 cctgtctact tgacaggggg cagttcactc tgagttaaaa tggtattctc ctagtaaatt
      121 aatatgttcc caacctaagg gcgacatatg gtgtaacaaa tcttcattaa agctacctgt
      181 ccgtttttta tattcaactg ctgttgttag gtggagagta ttccaaatac ttatagcatt
      241 gataattatg tttaaagcac tggctctttg caattgatgc tgtatggtgc gttctctaag
      301 ctcaccttgt tttccgaaga aaatagctct tgccaatcca ttcatggctt ctcctttatt
      361 caatcctctt tgtatttttc ttcttaatga ttcatccgat atataattca aaataaagat
      421 cgttttttct attcggccca tctcacgtaa ggctgtagct aagctgtttt gtcttgaata
      481 ggaacctagc ttccccataa taagggatgc tgaaactgtt ccctccctta tagaatgagc
      541 taatcgcaaa acatcctcat aattttcttt aatgaccttt gtatttattt gtccacgtaa
      601 aatggcttct agttttggat actcacttgc tttatctatc gtaaataatt ttgagtccga
      661 taaatccctt attcttgggg caaatttaaa tcctaataaa tgagtcagtc cgaatatttg
      721 gtcagtgtaa ccggcagtgt ctgtataatg ttcctctatg tttagatccg tctcatgatg
      781 taacaaacca tccaaaacat gaatcgcatc tcttgaatta gtatgaataa tctttgtgta
      841 gtaagaagag aattgatcac ttgtaaatcg gtagatggtg gctccttttc cagttccata
      901 atgtggattt gcatctgcat gtagtgatga aacacctagc tgcattctca taccatctga
      961 cgaagatgtt gtaccgtcgc cccaatagaa aggcaattgt aatttatgat gaaagtttac
     1021 taatatggct tgggctttat tcatggcatc ttcatacatg cgccattgag atacattggc
     1081 tagttgctta tatgtaagtc cgggtgtggc ttcggccatc ttgctcaagc caatattcat
     1141 tcccattcct aaaagggcag ccatgataat gattgtttct tccttatctg gttttcgatt
     1201 attggaagca tgagtgaatt gctcatgaaa tcctgttata tgggccacat ccatgagtaa
     1261 atcagttaat tttattcttg gtagcatctg ataaaggctt gcactaaatt tttttgcttc
     1321 ttctggaaca tctttttcta agcgtgcaag tgatagcttt cctttttcaa gagaaacccc
     1381 atctaactta ttggaattgg cagctaacca ctttaacctt tcattaaagc tgctggttct
     1441 ctccgttata taatcttcga atgataaact aactgataat ctcgtattcc ccttcgattg
     1501 attccatgta tcttccgaaa acaaatattc ctcaaaatcc ctatattgtc tgctgccaac
     1561 aatggaaaca tctcctgccc gaacatgctc ccgaagttct gttaaaacag ccatttcata
     1621 gtaatgacga ttaattgttg taccatcatc ctcgtataaa tgtcttttcc atcgttttga
     1681 aataaaatcc acaggtgagt catcaggcac ttttcgcttt ccagattcgt tcattcctcg
     1741 gataatctca acagcttgta aaagtggctc atttgccttt gtagaatgaa attccaatac
     1801 tcttaatagc gttggcgtat attttcttag tgaataaaac cgtttttgca gtaagtctaa
     1861 ataatcatag tcggcaggac gtgcaagttc ctgagcctct tctactgaag agacaaaggt
     1921 attccattca ataaccgatt ctaaaacctt aaaaacgtct aatttttcct ctcttgcttt
     1981 aattaatgct tgtccgatgt tcgtaaagtg tataactttc tcatttagct ttttaccgtt
     2041 ttgtttctgg atttcctctt gagccttacg accttttgat aacaaactaa gtatttgcct
     2101 atcatgaatt tcaaacgctt tatccgttag ctcctgagta agttgtaata aatagatggt
     2161 taatatcgaa taacgtttat tttcttgaaa gtcacggaat gcatacggct cgtatcttga
     2221 gcctaagcga gacagctgca acaggcggtt acggtgcaaa tgactaattt gcactgtttc
     2281 taaatccatt cctcgtatgt attcgagtcg ttctattatt tttagaaaag tttcgggtga
     2341 aggatgaccc ggtggctctt ttaaccaacc caatatcgtt ttattggatt cggatggatg
     2401 ctgcgaggta ataatccctt caagcttttc tttttgctca tttgttagag atttactaac
     2461 cgtattaaat agcttctttt cagccattgc ccttgcttcc cacaccattc tttcaagtgt
     2521 agtgatagca ggcagtataa ttttgttttt tcttagaaaa tctatgcatt catgcagtag
     2581 atgaatggca tcaccatttt ccaaagctaa ttgatgaagg tacttaaatg tcattcgata
     2641 ttcactcagg gtaaaagtta caaagtcgta ttcacttcga atttctttca aatgatccca
     2701 aagtgtattt tccctttgag gataatgatc aagcgaggat ggactaacac caatctgttt
     2761 cgatatatat tgtatgaccg aatctgggat gcttttgata tgagtgtatg gccaaccggg
     2821 ataccgaaga acagctaatt gaacagcaaa tcctaaacgg ttttcttccc tccttcgctt
     2881 attaactatt tctaaatccc gtttggaaaa agtgaagtag gtccccagta tccattcatc
     2941 ttcagggatt tgcataaaag cctgtctctg ttccggtgta agcaattctc tacctctcgc
     3001 aattttcatt cagtatcatt ccatttctgt attttcaatt tattagttca attatatatc
     3061 aatagagtgt actctattga tacaaatgta gtagactgat aaaatcatag ttaagagcgt
     3121 ctcataagac ttgtctcaaa aatgaggtga tattttgcgg aaaatcggtt atattcgtgt
     3181 cagttcgact aaccagaatc cttcaagaca atttcagcag ttgaacgaga tcggaatgga
     3241 tattatatat gaagagaaag tttcaggagc aacaaaggat cgcgagcaac ttcaaaaagt
     3301 gttagacgat ttacaggaag atgacatcat ttatgttaca gacttaactc gaatcactcg
     3361 tagtacacaa gatctatttg aattaatcga taacatacga gataaaaagg caagtttaaa
     3421 atcactaaaa gatacatggc ttgatttatc agaagataat ccatacagcc aattcttaat
     3481 tactgtaatg gctggtgtta accaattaga gcgagatctt attcggatga gacaacgtga
     3541 agggattgaa ttggctaaga aagaaggaaa gtttaaaggt cgattaaaga agtatcataa
     3601 aaatcacgca ggaatgaatt atgcggtaaa gctatataaa gaaggaaata tgactgtaaa
     3661 tcaaatttgt gaaattacta atgtatctag ggcttcatta tacaggaaat tatcagaagt
     3721 gaataattag ccattctgta ttccgctaat gggcaatatt tttaaagaag aaaaggaaac
     3781 tataaaatat taacagcctc ctagcgatgc cgaaaagccc tttgataaaa aaagaatcat
     3841 catcttaaga aattcttagt catttattat gtaaatgctt ataaattcgg ccctataatc
     3901 tgataaatta ttaagggcaa acttatgtga aagggtgata actatgagcg ataaaatact
     3961 tattgtggat gatgaacatg aaattgccga tttggttgaa ttatacttaa aaaacgagaa
     4021 ttatacggtt ttcaaatact ataccgccaa agaagcattg gaatgtatag acaagtctga
     4081 gattgacctt gccatattgg acatcatgct tcccggcaca agcggcctta ctatctgtca
     4141 aaaaataagg gacaagcaca cctatccgat tatcatgctg accgggaaag atacagaggt
     4201 agataaaatt acagggttaa caatcggcgc ggatgattat ataacgaagc cctttcgccc
     4261 actggagtta attgctcggg taaaggccca gttgcgccga tacaaaaaat tcagtggagt
     4321 aaaggagcag aacgaaaatg ttatcgtcca ctccggcctt gtcattaatg ttaacaccca
     4381 tgagtgttat ctgaacgaga agcagttatc ccttactccc accgagtttt caatactgcg
     4441 aatcctctgt gaaaacaagg ggaatgtggt tagctccgag ctgctatttc atgagatatg
     4501 gggcgacgaa tatttcagca agagcaacaa caccatcacc gtgcatatcc ggcatttgcg
     4561 cgaaaaaatg aacgacacca ttgataatcc gaaatatata aaaacggtat ggggggttgg
     4621 ttataaaatt gaaaaataaa aaaaacgact attccaaact agaacgaaaa ctttacatgt
     4681 atatcgttgc aattgttgtg gtagcaattg tattcgtgtt gtatattcgt tcaatgatcc
     4741 gagggaaact tggggattgg atcttaagta ttttggaaaa caaatatgac ttaaatcacc
     4801 tggacgcgat gaaattatat caatattcca tacggaacaa tatagatatc tttatttatg
     4861 tggcgattgt cattagtatt cttattctat gtcgcgtcat gctttcaaaa ttcgcaaaat
     4921 actttgacga gataaatacc ggcattgatg tacttattca gaacgaagat aaacaaattg
     4981 agctttctgc ggaaatggat gttatggaac aaaagctcaa cacattaaaa cggactctgg
     5041 aaaagcgaga gcaggatgca aagctggccg aacaaagaaa aaatgacgtt gttatgtact
     5101 tggcgcacga tattaaaacg ccccttacat ccattatcgg ttatttgagc ctgcttgacg
     5161 aggctccaga catgccggta gatcaaaagg caaagtatgt gcatatcacg ttggacaaag
     5221 cgtatcgact cgaacagcta atcgacgagt tttttgagat tacacggtat aacctacaaa
     5281 cgataacgct aacaaaaacg cacatagacc tatactatat gctggtgcag atgaccgatg
     5341 aattttatcc tcagctttcc gcacatggaa aacaggcggt tattcacgcc cccgaggatc
     5401 tgaccgtgtc cggcgaccct gataaactcg cgagagtctt taacaacatt ttgaaaaacg
     5461 ccgctgcata cagtgaggat aacagcatca ttgacattac cgcgggcctc tccggggatg
     5521 tggtgtcaat cgaattcaag aacactggaa gcatcccaaa agataagcta gctgccatat
     5581 ttgaaaagtt ctataggctg gacaatgctc gttcttccga tacgggtggc gcgggacttg
     5641 gattggcgat tgcaaaagaa attattgttc agcatggagg gcagatttac gcggaaagca
     5701 atgataacta tacgacgttt agggtagagc ttccagggtt ctgttgcaaa gttttaaata
     5761 aagaataaaa tccct
//
LOCUS       DACGLH010000241         3970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.152, whole genome shotgun sequence.
ACCESSION   DACGLH010000241 DACGLH010000000
VERSION     DACGLH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.152"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXT21_003265"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXT21_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ4870581.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IXT21_003266"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IXT21_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ4870582.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1254 a          613 c          818 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DACGLH010000242         3970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.153, whole genome shotgun sequence.
ACCESSION   DACGLH010000242 DACGLH010000000
VERSION     DACGLH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.153"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXT21_003267"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXT21_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ4870583.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IXT21_003268"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IXT21_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ4870584.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1253 a          613 c          819 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DACGLH010000243         3604 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.156, whole genome shotgun sequence.
ACCESSION   DACGLH010000243 DACGLH010000000
VERSION     DACGLH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.156"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            complement(<1..164)
                     /locus_tag="IXT21_003269"
     CDS             complement(<1..164)
                     /locus_tag="IXT21_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4870585.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IXT21_003270"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IXT21_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ4870586.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="IXT21_003271"
     CDS             complement(963..2915)
                     /locus_tag="IXT21_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ4870587.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3218)
                     /locus_tag="IXT21_003272"
     CDS             complement(2922..3218)
                     /locus_tag="IXT21_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4870588.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3393..>3604)
                     /locus_tag="IXT21_003273"
     CDS             complement(3393..>3604)
                     /locus_tag="IXT21_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4870589.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1115 a          622 c          583 g         1284 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tctttttttt
     3421 gtatattcaa ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa
     3481 taaaatttat tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt
     3541 ccatgataaa gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt
     3601 tcca
//
LOCUS       DACGLH010000244         2949 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.166, whole genome shotgun sequence.
ACCESSION   DACGLH010000244 DACGLH010000000
VERSION     DACGLH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.166"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            297..1265
                     /locus_tag="IXT21_003274"
     CDS             297..1265
                     /locus_tag="IXT21_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ4870590.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1258..2289
                     /gene="vanA"
                     /locus_tag="IXT21_003275"
     CDS             1258..2289
                     /gene="vanA"
                     /locus_tag="IXT21_003275"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ4870591.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2295..2903
                     /locus_tag="IXT21_003276"
     CDS             2295..2903
                     /locus_tag="IXT21_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ4870592.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          876 a          562 c          714 g          797 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg cttccagcga tgccagactt
       61 ggttgataaa aggaggtcct aagagatgta tataattttt taggaaaatc tcaaggttat
      121 ctttactttt tcttaggaaa ttaacaattt aatattaaga aacggctcgt tcttacacgg
      181 tagacttaat accgtaagaa cgagccgttt tcgttcttca gagaaagatt tgacaagatt
      241 accattggca tccccgtttt atttggtgcc tttcacagaa agggttggtc ttaattatga
      301 ataacatcgg cattactgtt tatggatgtg agcaggatga ggcagatgca ttccatgctc
      361 tttcgcctcg ctttggcgtt atggcaacga taattaacgc caacgtgtcg gaatccaacg
      421 ccaaatccgc gcctttcaat caatgtatca gtgtgggaca taaatcagag atttccgcct
      481 ctattcttct tgcgctgaag agagccggtg tgaaatatat ttctacccga agcatcggct
      541 gcaatcatat agatacaact gctgctaaga gaatgggcat cactgtcgac aatgtggcgt
      601 actcgccgga tagcgttgcc gattatacta tgatgctaat tcttatggca gtacgcaacg
      661 taaaatcgat tgtgcgctct gtggaaaaac atgatttcag gttggacagc gaccgtggca
      721 aggtactcag cgacatgaca gttggtgtgg tgggaacggg ccagataggc aaagcggtta
      781 ttgagcggct gcgaggattt ggatgtaaag tgttggctta tagtcgcagc cgaagtatag
      841 aggtaaacta tgtaccgttt gatgagttgc tgcaaaatag cgatatcgtt acgcttcatg
      901 tgccgctcaa tacggatacg cactatatta tcagccacga acaaatacag agaatgaagc
      961 aaggagcatt tcttatcaat actgggcgcg gtccacttgt agatacctat gagttggtta
     1021 aagcattaga aaacgggaaa ctgggcggtg ccgcattgga tgtattggaa ggagaggaag
     1081 agtttttcta ctctgattgc acccaaaaac caattgataa tcaattttta cttaaacttc
     1141 aaagaatgcc taacgtgata atcacaccgc atacggccta ttataccgag caagcgttgc
     1201 gtgataccgt tgaaaaaacc attaaaaact gtttggattt tgaaaggaga caggagcatg
     1261 aatagaataa aagttgcaat actgtttggg ggttgctcag aggagcatga cgtatcggta
     1321 aaatctgcaa tagagatagc cgctaacatt aataaagaaa aatacgagcc gttatacatt
     1381 ggaattacga aatctggtgt atggaaaatg tgcgaaaaac cttgcgcgga atgggaaaac
     1441 gacaattgct attcagctgt actctcgccg gataaaaaaa tgcacggatt acttgttaaa
     1501 aagaaccatg aatatgaaat caaccatgtt gatgtagcat tttcagcttt gcatggcaag
     1561 tcaggtgaag atggatccat acaaggtctg tttgaattgt ccggtatccc ttttgtaggc
     1621 tgcgatattc aaagctcagc aatttgtatg gacaaatcgt tgacatacat cgttgcgaaa
     1681 aatgctggga tagctactcc cgccttttgg gttattaata aagatgatag gccggtggca
     1741 gctacgttta cctatcctgt ttttgttaag ccggcgcgtt caggctcatc cttcggtgtg
     1801 aaaaaagtca atagcgcgga cgaattggac tacgcaattg aatcggcaag acaatatgac
     1861 agcaaaatct taattgagca ggctgtttcg ggctgtgagg tcggttgtgc ggtattggga
     1921 aacagtgccg cgttagttgt tggcgaggtg gaccaaatca ggctgcagta cggaatcttt
     1981 cgtattcatc aggaagtcga gccggaaaaa ggctctgaaa acgcagttat aaccgttccc
     2041 gcagaccttt cagcagagga gcgaggacgg atacaggaaa cggcaaaaaa aatatataaa
     2101 gcgctcggct gtagaggtct agcccgtgtg gatatgtttt tacaagataa cggccgcatt
     2161 gtactgaacg aagtcaatac tctgcccggt ttcacgtcat acagtcgtta tccccgtatg
     2221 atggccgctg caggtattgc acttcccgaa ctgattgacc gcttgatcgt attagcgtta
     2281 aaggggtgat aagcatggaa ataggattta cttttttaga tgaaatagta cacggtgttc
     2341 gttgggacgc taaatatgcc acttgggata atttcaccgg aaaaccggtt gacggttatg
     2401 aagtaaatcg cattgtaggg acatacgagt tggctgaatc gcttttgaag gcaaaagaac
     2461 tggctgctac ccaagggtac ggattgcttc tatgggacgg ttaccgtcct aagcgtgctg
     2521 taaactgttt tatgcaatgg gctgcacagc cggaaaataa cctgacaaag gaaagttatt
     2581 atcccaatat tgaccgaact gagatgattt caaaaggata cgtggcttca aaatcaagcc
     2641 atagccgcgg cagtgccatt gatcttacgc tttatcgatt agacacgggt gagcttgtac
     2701 caatggggag ccgatttgat tttatggatg aacgctctca tcatgcggca aatggaatat
     2761 catgcaatga agcgcaaaat cgcagacgtt tgcgctccat catggaaaac agtgggtttg
     2821 aagcatatag cctcgaatgg tggcactatg tattaagaga cgaaccatac cccaatagct
     2881 attttgattt ccccgttaaa taaactttta acggttctgt tgcaaagttt taaataaaga
     2941 ataaaatcc
//
LOCUS       DACGLH010000245         2014 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.182, whole genome shotgun sequence.
ACCESSION   DACGLH010000245 DACGLH010000000
VERSION     DACGLH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.182"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            216..1127
                     /locus_tag="IXT21_003277"
     CDS             216..1127
                     /locus_tag="IXT21_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ4870593.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1280..1765
                     /gene="vanZ"
                     /locus_tag="IXT21_003278"
     CDS             1280..1765
                     /gene="vanZ"
                     /locus_tag="IXT21_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ4870594.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          716 a          275 c          386 g          637 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgt atcacttatg aaaattcatc
       61 tacattggtg ataatagtaa atccagtagg gcgaaataat tgactgtaat ttacggggca
      121 aaacggcaca atctcaaacg agattgtgcc gtttaagggg aagattctag aaatatttca
      181 tacttccaac tatatagtta aggaggagac tgaaaatgaa gaagttgttt tttttattgt
      241 tattgttatt cttaatatac ttaggttatg actacgttaa tgaagcactg ttttctcagg
      301 aaaaagtcga atttcaaaat tatgatcaaa atcccaaaga acatttagaa aatagtggga
      361 cttctgaaaa tacccaagag aaaacaatta cagaagaaca ggtttatcaa ggaaatctgc
      421 tattaatcaa tagtaaatat cctgttcgcc aagaaagtgt gaagtcagat atcgtgaatt
      481 tatctaaaca tgacgaatta ataaatggat acgggttgct tgatagtaat atttatatgt
      541 caaaagaaat agcacaaaaa ttttcagaga tggtcaatga tgctgtaaag ggtggcgtta
      601 gtcattttat tattaatagt ggctatcgag actttgatga gcaaagtgtg ctttaccaag
      661 aaatgggggc tgagtatgcc ttaccagcag gttatagtga gcataattca ggtttatcac
      721 tagatgtagg atcaagcttg acgaaaatgg aacgagcccc tgaaggaaag tggatagaag
      781 aaaatgcttg gaaatacggg ttcattttac gttatccaga ggacaaaaca gagttaacag
      841 gaattcaata tgaaccatgg catattcgct atgttggttt accacatagt gcgattatga
      901 aagaaaagaa tttcgttctc gaggaatata tggattacct aaaagaagaa aaaaccattt
      961 ctgttagtgt aaatggggaa aaatatgaga tcttttatta tcctgttact aaaaatacca
     1021 ccattcatgt gccgactaat cttcgttatg agatatcagg aaacaatata gacggtgtaa
     1081 ttgtgacagt gtttcccgga tcaacacata ctaattcaag gaggtaagga tggcggaatg
     1141 aaaccaacga aattaatgaa cagcattatt gtactagcac ttttggggta acgttagctt
     1201 tttaatttaa aacccacgtt aactaggaca ttgctatact aatgatacaa cttaaacaaa
     1261 agaattagag gaaattatat tgggaaaaat attatctaga ggattgctag ctttatattt
     1321 agtgacacta atctggttag tgttattcaa attacaatac aatattttat cagtatttaa
     1381 ttatcatcaa agaagtctta acttgactcc atttactgct actgggaatt tcagagagat
     1441 gatagataat gttataatct ttattccatt tggcttgctt ttgaatgtca attttaaaga
     1501 aatcggattt ttacctaagt ttgcttttgt actggtttta agtcttactt ttgaaataat
     1561 tcaatttatc ttcgctattg gagcgacaga cataacagat gtaattacaa atactgttgg
     1621 aggctttctt ggactgaaat tatatggttt aagcaataag catatgaatc aaaaaaaatt
     1681 agacagagtt attatttttg taggtatact tttgctcgta ttattgctcg tttaccgtac
     1741 ccatttaaga ataaattacg tgtaagatgt ctaaatcaag caatctgatc tttcatacac
     1801 ataaagatat tgaatgaatt ggattagatg gaaaacggga tgtggggaaa ctcgcccgta
     1861 ggtgtgaagt gaggggaaaa ccggtgataa agtaaaaagc ttacctaaca ctatagtaac
     1921 aaagaaagcc caattatcaa ttttagtgct gaggaattgg tctctttaat aaatttggtt
     1981 ctgttgcaaa gttttaaatc tactatcaaa taag
//
LOCUS       DACGLH010000246          948 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.215, whole genome shotgun sequence.
ACCESSION   DACGLH010000246 DACGLH010000000
VERSION     DACGLH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.215"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            110..844
                     /locus_tag="IXT21_003279"
     CDS             110..844
                     /locus_tag="IXT21_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ4870595.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          384 a          125 c          132 g          307 t
ORIGIN      
        1 atgaggttga acttttacac aagattgtgg acttgactaa caaaaaataa gtggttataa
       61 tggattgtta atataaacat tcattataac ctcataggag tgggttataa tgaacaaaaa
      121 aaatataaaa gatagtcaaa actttattac ttcaaagcat catataaatg aaattttgag
      181 aaatgtacat ttaaatacaa atgataatat tattgagatt ggttcaggga aaggtcattt
      241 cacgtttgaa ttagctaaaa ggtgtaatta tgtaaccgcc attgaaatag atcctaaatt
      301 atgtaggata actaaaaaca aacttattga atatgagaac ttccaggtta tcaataaaga
      361 tattttacaa tttaagtttc ctaaaaataa gtcatataag atatatggaa atatacccta
      421 caatataagt acagatataa ttcgaaaaat tgtttttgaa agcacagcta cagaaagtta
      481 tttaatagtg gaatatggat ttgctaaaag gttgctaaat acaaatcgtt cactagcact
      541 atttttaatg acagaagttg atatatccat attaagtaaa atccctagag aatactttca
      601 tccaaaacct agagttaata gctcgttaat tgtattaaaa agacaccctt caaaaatatc
      661 actcaaagat agaaaacaat atgaaaattt tgttatgaaa tgggttaaca aagaatacat
      721 aaaactattt tccaaaaacc aattttatca agccttaaaa tatgcaagaa ttgacgattt
      781 aaacaatatt agctttgaac aattcttgtc tcttttcaat agctataaat tatttaatag
      841 ataagttaag gggtgcataa actgcatccc ttaacttatc ttattttgct taaacaatct
      901 tggcccgtaa actgcataac tcatatgtaa atcgctcctt tttaggtg
//
LOCUS       DACGLH010000247          894 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.217, whole genome shotgun sequence.
ACCESSION   DACGLH010000247 DACGLH010000000
VERSION     DACGLH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.217"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            <1..775
                     /gene="aph(2'')-I"
                     /locus_tag="IXT21_003280"
     CDS             <1..775
                     /gene="aph(2'')-I"
                     /locus_tag="IXT21_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAQ4870596.1"
                     /translation="DFFIYTKYFILSKGYAKEKAIYNFLNTNLETNVKIPNIEYSYIS
                     DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDN
                     KQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLL
                     DGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEY
                     QDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          385 a           67 c          142 g          300 t
ORIGIN      
        1 ggattttttc atttacacaa aatattttat actctcaaaa ggttatgcaa aagaaaaagc
       61 aatatataat tttttaaata caaatttaga aactaatgta aaaattccta atattgaata
      121 ttcgtatatt agtgatgaat tatctatact aggttataaa gaaattaaag gaactttttt
      181 aacaccagaa atttattcta ctatgtcaga agaagaacaa aatttgttaa aacgagatat
      241 tgccagtttt ttaagacaaa tgcacggttt agattataca gatattagtg aatgtactat
      301 tgataataaa caaaatgtat tagaagagta tatattgttg cgtgaaacta tttataatga
      361 tttaactgat atagaaaaag attatataga aagttttatg gaaagactaa atgcaacaac
      421 agtttttgag ggtaaaaagt gtttatgcca taatgatttt agttgtaatc atctattgtt
      481 agatggcaat aatagattaa ctggaataat tgattttgga gattctggaa ttatagatga
      541 atattgtgat tttatatact tacttgaaga tagtgaagaa gaaataggaa caaattttgg
      601 agaagatata ttaagaatgt atggaaatat agatattgag aaagcaaaag aatatcaaga
      661 tatagttgaa gaatattatc ctattgaaac tattgtttat ggaattaaaa atattaaaca
      721 ggaatttatc gaaaatggta gaaaagaaat ttataaaagg acttataaag attgattata
      781 taatatatga aaagctatta taaaagacat tagtattaaa tagtttaaaa aaatgaaaaa
      841 taataaagga agtgagtcaa gtccagactc ctgtgtaaaa tgctatacaa tgtt
//
LOCUS       DACGLH010000248          655 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0573
            SAMEA3304444-rid9553203.guided.232, whole genome shotgun sequence.
ACCESSION   DACGLH010000248 DACGLH010000000
VERSION     DACGLH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304444
            Sequence Read Archive: ERR985060
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702221.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702221.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 03:17:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,222
            Genes (coding)                    :: 3,147
            CDSs (with protein)               :: 3,147
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304444-rid9553203.guided.232"
                     /strain="VREN0573"
                     /db_xref="taxon:1352"
     gene            1..628
                     /locus_tag="IXT21_003281"
                     /pseudo
     CDS             1..628
                     /locus_tag="IXT21_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT          262 a           62 c          117 g          214 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaaggaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttggg agcgtcaata attttgtgta aataaattgt cctcc
//
